LOCUS       DAVKJD010000001        77134 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKJD010000001 DAVKJD010000000
VERSION     DAVKJD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 77134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.001"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            118..1599
                     /locus_tag="VW157_000001"
     CDS             118..1599
                     /locus_tag="VW157_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9551397.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1961..2956
                     /locus_tag="VW157_000002"
     CDS             1961..2956
                     /locus_tag="VW157_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9551398.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFWGDKTTKKI"
     gene            3084..4394
                     /locus_tag="VW157_000003"
     CDS             3084..4394
                     /locus_tag="VW157_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9551399.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            4490..5392
                     /locus_tag="VW157_000004"
     CDS             4490..5392
                     /locus_tag="VW157_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9551400.1"
                     /translation="MSKETVVDTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVYYIYQQGFTDRLVGYSSS
                     IALVFTTLIGMISLVQRRVLKEDD"
     gene            5394..6239
                     /locus_tag="VW157_000005"
     CDS             5394..6239
                     /locus_tag="VW157_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9551401.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            6249..7568
                     /locus_tag="VW157_000006"
     CDS             6249..7568
                     /locus_tag="VW157_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9551402.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKIEQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVATKHKDNVGCIVLLGSDNLVEWQFESIFLKGREHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(7776..7848)
                     /locus_tag="VW157_000007"
     tRNA            complement(7776..7848)
                     /locus_tag="VW157_000007"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7813..7815),aa:Lys,seq:ctt)
     gene            complement(8112..9383)
                     /locus_tag="VW157_000008"
     CDS             complement(8112..9383)
                     /locus_tag="VW157_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9551403.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            9653..9892
                     /locus_tag="VW157_000009"
     CDS             9653..9892
                     /locus_tag="VW157_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551404.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVYSNNGLKKYEK"
     gene            10057..10236
                     /locus_tag="VW157_000010"
     CDS             10057..10236
                     /locus_tag="VW157_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551405.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10379..10552
                     /locus_tag="VW157_000011"
     CDS             10379..10552
                     /locus_tag="VW157_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9551406.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            10589..11041
                     /locus_tag="VW157_000012"
     CDS             10589..11041
                     /locus_tag="VW157_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9551407.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11332..11802
                     /locus_tag="VW157_000013"
     CDS             11332..11802
                     /locus_tag="VW157_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9551408.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            11821..12909
                     /locus_tag="VW157_000014"
     CDS             11821..12909
                     /locus_tag="VW157_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU9551409.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            12890..13318
                     /locus_tag="VW157_000015"
     CDS             12890..13318
                     /locus_tag="VW157_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9551410.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            13456..14406
                     /locus_tag="VW157_000016"
     CDS             13456..14406
                     /locus_tag="VW157_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9551411.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTALA
                     EEDWADNRKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            14408..15151
                     /locus_tag="VW157_000017"
     CDS             14408..15151
                     /locus_tag="VW157_000017"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9551412.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDFIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            15162..16958
                     /gene="pepF"
                     /locus_tag="VW157_000018"
     CDS             15162..16958
                     /gene="pepF"
                     /locus_tag="VW157_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9551413.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            17205..17759
                     /locus_tag="VW157_000019"
     CDS             17205..17759
                     /locus_tag="VW157_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU9551414.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLSNQGL
                     HYLSKEKDTTDFSSQYGWVHAFAHGADLLTEVVCHPDFPINRIHEVFDILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDDLKEAIQSFQY"
     gene            17870..18538
                     /locus_tag="VW157_000020"
     CDS             17870..18538
                     /locus_tag="VW157_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9551415.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(18659..18736)
                     /locus_tag="VW157_000021"
                     /pseudo
     CDS             complement(18659..18736)
                     /locus_tag="VW157_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(18748..19281)
                     /gene="queF"
                     /locus_tag="VW157_000022"
     CDS             complement(18748..19281)
                     /gene="queF"
                     /locus_tag="VW157_000022"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9551416.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(19586..19900)
                     /gene="trxA"
                     /locus_tag="VW157_000023"
     CDS             complement(19586..19900)
                     /gene="trxA"
                     /locus_tag="VW157_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9551417.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            complement(19917..20138)
                     /locus_tag="VW157_000024"
     CDS             complement(19917..20138)
                     /locus_tag="VW157_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9551418.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            complement(20238..20618)
                     /locus_tag="VW157_000025"
     CDS             complement(20238..20618)
                     /locus_tag="VW157_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9551419.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            complement(20762..21715)
                     /locus_tag="VW157_000026"
     CDS             complement(20762..21715)
                     /locus_tag="VW157_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9551420.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAISVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIESYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYGQ"
     gene            complement(21783..22992)
                     /gene="sstT"
                     /locus_tag="VW157_000027"
                     /pseudo
     CDS             complement(21783..22992)
                     /gene="sstT"
                     /locus_tag="VW157_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            23131..23691
                     /locus_tag="VW157_000028"
     CDS             23131..23691
                     /locus_tag="VW157_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9551421.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            23748..24656
                     /locus_tag="VW157_000029"
     CDS             23748..24656
                     /locus_tag="VW157_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9551422.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            24608..25198
                     /locus_tag="VW157_000030"
     CDS             24608..25198
                     /locus_tag="VW157_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9551423.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            25201..26307
                     /gene="yqeH"
                     /locus_tag="VW157_000031"
     CDS             25201..26307
                     /gene="yqeH"
                     /locus_tag="VW157_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9551424.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            26521..26832
                     /gene="yhbY"
                     /locus_tag="VW157_000032"
     CDS             26521..26832
                     /gene="yhbY"
                     /locus_tag="VW157_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9551425.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            26884..27513
                     /locus_tag="VW157_000033"
     CDS             26884..27513
                     /locus_tag="VW157_000033"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9551426.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            27513..28106
                     /gene="yqeK"
                     /locus_tag="VW157_000034"
     CDS             27513..28106
                     /gene="yqeK"
                     /locus_tag="VW157_000034"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9551427.1"
                     /translation="MAYQDYINCSREVLLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            28107..28607
                     /locus_tag="VW157_000035"
     CDS             28107..28607
                     /locus_tag="VW157_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9551428.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            28620..28973
                     /gene="rsfS"
                     /locus_tag="VW157_000036"
     CDS             28620..28973
                     /gene="rsfS"
                     /locus_tag="VW157_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9551429.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            29076..29822
                     /locus_tag="VW157_000037"
     CDS             29076..29822
                     /locus_tag="VW157_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9551430.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKEKKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            29832..30929
                     /locus_tag="VW157_000038"
     CDS             29832..30929
                     /locus_tag="VW157_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9551431.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQSMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVEAVATKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETS"
     gene            31167..31421
                     /locus_tag="VW157_000039"
     CDS             31167..31421
                     /locus_tag="VW157_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU9551432.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSIQVHVIEE"
     gene            31425..31679
                     /locus_tag="VW157_000040"
     CDS             31425..31679
                     /locus_tag="VW157_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU9551433.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            31870..33483
                     /locus_tag="VW157_000041"
     CDS             31870..33483
                     /locus_tag="VW157_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9551434.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            33613..34239
                     /gene="gmk"
                     /locus_tag="VW157_000042"
     CDS             33613..34239
                     /gene="gmk"
                     /locus_tag="VW157_000042"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9551435.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            34264..34578
                     /locus_tag="VW157_000043"
     CDS             34264..34578
                     /locus_tag="VW157_000043"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9551436.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            34644..37040
                     /locus_tag="VW157_000044"
     CDS             34644..37040
                     /locus_tag="VW157_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9551437.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHTQLEGVEISTRAKKKLELRDYLLSHPESSSLAN
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQAHDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            37053..37988
                     /locus_tag="VW157_000045"
     CDS             37053..37988
                     /locus_tag="VW157_000045"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9551438.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPELQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            37981..39294
                     /gene="rsmB"
                     /locus_tag="VW157_000046"
     CDS             37981..39294
                     /gene="rsmB"
                     /locus_tag="VW157_000046"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9551439.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDNWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFTNGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            39309..40049
                     /locus_tag="VW157_000047"
     CDS             39309..40049
                     /locus_tag="VW157_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9551440.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            40046..42025
                     /gene="pknB"
                     /locus_tag="VW157_000048"
     CDS             40046..42025
                     /gene="pknB"
                     /locus_tag="VW157_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9551441.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            42140..42898
                     /locus_tag="VW157_000049"
     CDS             42140..42898
                     /locus_tag="VW157_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9551442.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            42900..43349
                     /locus_tag="VW157_000050"
     CDS             42900..43349
                     /locus_tag="VW157_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9551443.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            43411..44037
                     /locus_tag="VW157_000051"
                     /pseudo
     CDS             43411..44037
                     /locus_tag="VW157_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            44105..44353
                     /locus_tag="VW157_000052"
     CDS             44105..44353
                     /locus_tag="VW157_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551444.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            44484..45656
                     /locus_tag="VW157_000053"
     CDS             44484..45656
                     /locus_tag="VW157_000053"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9551445.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIVLYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            45656..46930
                     /locus_tag="VW157_000054"
     CDS             45656..46930
                     /locus_tag="VW157_000054"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9551446.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(46965..47930)
                     /locus_tag="VW157_000055"
     CDS             complement(46965..47930)
                     /locus_tag="VW157_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9551447.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(47911..49365)
                     /locus_tag="VW157_000056"
     CDS             complement(47911..49365)
                     /locus_tag="VW157_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9551448.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            49742..51625
                     /locus_tag="VW157_000057"
     CDS             49742..51625
                     /locus_tag="VW157_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9551449.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            51774..52661
                     /locus_tag="VW157_000058"
     CDS             51774..52661
                     /locus_tag="VW157_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9551450.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGENIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(52981..53598)
                     /locus_tag="VW157_000059"
     CDS             complement(52981..53598)
                     /locus_tag="VW157_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9551451.1"
                     /translation="MSQDKQMKAASPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(54013..54201)
                     /locus_tag="VW157_000060"
                     /pseudo
     CDS             complement(54013..54201)
                     /locus_tag="VW157_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(54711..54791)
                     /locus_tag="VW157_000061"
                     /pseudo
     CDS             complement(54711..54791)
                     /locus_tag="VW157_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            54908..55801
                     /locus_tag="VW157_000062"
     CDS             54908..55801
                     /locus_tag="VW157_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9551452.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            55798..56997
                     /locus_tag="VW157_000063"
     CDS             55798..56997
                     /locus_tag="VW157_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9551453.1"
                     /translation="MRNMWVVIKETYLRHVKSWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGEISLENGIKFEVTGSLNELQNQLNRSTASLSQEQEKRLAQTVQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(57350..58117)
                     /locus_tag="VW157_000064"
     CDS             complement(57350..58117)
                     /locus_tag="VW157_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551454.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(58134..58829)
                     /locus_tag="VW157_000065"
     CDS             complement(58134..58829)
                     /locus_tag="VW157_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9551455.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(58834..59199)
                     /locus_tag="VW157_000066"
     CDS             complement(58834..59199)
                     /locus_tag="VW157_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9551456.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            59339..59812
                     /gene="nrdR"
                     /locus_tag="VW157_000067"
     CDS             59339..59812
                     /gene="nrdR"
                     /locus_tag="VW157_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9551457.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            59813..60982
                     /locus_tag="VW157_000068"
     CDS             59813..60982
                     /locus_tag="VW157_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9551458.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRDR
                     GQKAKTQKQNQTAPEKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            60983..61879
                     /gene="dnaI"
                     /locus_tag="VW157_000069"
     CDS             60983..61879
                     /gene="dnaI"
                     /locus_tag="VW157_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9551459.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPKEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            61876..62589
                     /locus_tag="VW157_000070"
     CDS             61876..62589
                     /locus_tag="VW157_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9551460.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            62603..63913
                     /gene="der"
                     /locus_tag="VW157_000071"
     CDS             62603..63913
                     /gene="der"
                     /locus_tag="VW157_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9551461.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            64004..64339
                     /locus_tag="VW157_000072"
     CDS             64004..64339
                     /locus_tag="VW157_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551462.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            64480..64815
                     /locus_tag="VW157_000073"
     CDS             64480..64815
                     /locus_tag="VW157_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551463.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMLDLRRE
                     LGKSKGGRH"
     gene            65000..65791
                     /locus_tag="VW157_000074"
     CDS             65000..65791
                     /locus_tag="VW157_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551464.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            65784..66425
                     /locus_tag="VW157_000075"
     CDS             65784..66425
                     /locus_tag="VW157_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551465.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            66429..67061
                     /locus_tag="VW157_000076"
                     /pseudo
     CDS             66429..67061
                     /locus_tag="VW157_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            67089..67376
                     /locus_tag="VW157_000077"
     CDS             67089..67376
                     /locus_tag="VW157_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551466.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWIAFFFLPWGSPILGGKIDLFS
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFILFAILPILGN"
     gene            67522..70035
                     /gene="secA"
                     /locus_tag="VW157_000078"
     CDS             67522..70035
                     /gene="secA"
                     /locus_tag="VW157_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9551467.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            70116..71147
                     /locus_tag="VW157_000079"
     CDS             70116..71147
                     /locus_tag="VW157_000079"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9551468.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            71149..72180
                     /locus_tag="VW157_000080"
     CDS             71149..72180
                     /locus_tag="VW157_000080"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9551469.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            72219..72587
                     /locus_tag="VW157_000081"
     CDS             72219..72587
                     /locus_tag="VW157_000081"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9551470.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            72577..73680
                     /locus_tag="VW157_000082"
     CDS             72577..73680
                     /locus_tag="VW157_000082"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9551471.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHDVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            73699..75714
                     /gene="recG"
                     /locus_tag="VW157_000083"
     CDS             73699..75714
                     /gene="recG"
                     /locus_tag="VW157_000083"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9551472.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAANFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLNWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQYGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            76031..77011
                     /locus_tag="VW157_000084"
     CDS             76031..77011
                     /locus_tag="VW157_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9551473.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     KDFHIPQVKYYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAVYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT        23039 a        14409 c        17381 g        22305 t
ORIGIN      
        1 tgttattata cgaaataaaa gattttagga aaagtcgtta tatcatgcta tacctattct
       61 ttgtggtata attgcaagag gtttaatccg ataatttata aagagagaaa gatattcatg
      121 agagatttat tatctaaaaa aagtcatagg caattagaat tattagaatt attatttgaa
      181 cataaacgtt ggtttcatcg ttctgaacta gcagagttat taaattgtac agaacgtgca
      241 gtcaaagatg atctatccca tgttaaatct gcttttcctg acttgatttt tcattcttct
      301 actaatggta tacgcattat taataccgat gatagtgata ttgaaatggt ttaccatcat
      361 ttctttaaac attcaactca tttttcgatt ttagaattca tcttctttaa tgaaggttgt
      421 caagctgaga gtatttgtaa agaattttat atcagttcat cttcgctcta tcgtattatt
      481 agccaaatca ataaagtgat taaaaggcaa tttcaatttg aagtcagtct gacccctgtt
      541 caaatcattg gaaatgagag agacattcgt tacttttttg cacaatattt ttcagaaaaa
      601 tattatttcc tagaatggcc atttgaaaat ttttcatcag agccactatc tcaattgtta
      661 gaattggttt ataaggaaac aagctttcca atgaatttgt caacgcatag aatgctaaag
      721 ttgctcctag ttacgaacct atatagaata aagtttggtc attttatgga agtagataaa
      781 gattctttta acgaccaaag tttggatttt ttaatgcagg cagaaggaat agaaggtgtt
      841 gctcagagtt ttgaatcaga atacaatatc tctttagatg aagaagttgt ttgccaatta
      901 tttgtgtctt actttcaaaa aatgtttttc atagatgaaa gtctctttat gaaatgcgta
      961 aaaaaggata gttatgttga aaaatcttac catctattga gtgattttat tgatcagatt
     1021 tcagtcaagt atcagattga aatggagaat aaggataatc tgatttggca tctgcataat
     1081 accgcacatc tgtatcgtca agagttgttt actgagttta ttttatttga tcaaaaaggg
     1141 aatacaatca ggaactttca aaatattttt ccaaaatttg tttcagatat aaaaaaagag
     1201 ctttctcact atttagagac tcttgaggtt tgttctagtt caatgatggt aaatcatcta
     1261 tcttatacct tcatcactca taccaaacat ttggtgatta atttgctaca aaatcagcct
     1321 aaactaaagg ttttggttat gagtaatttt gatcagtatc atgcaaaatt cgttgcagag
     1381 acactttctt attactgtag caataatttt gaactggagg tttggaccga attagaatta
     1441 tcaaaggaat ctttagaaga ttcaccttat gatattatta tttccaattt tattattcct
     1501 cctattgaaa ataagagact catctattca aataatataa acacggtctc actcatatac
     1561 ttgttaaatg ccatgatgtt tattcgatta gatgagtaac caaaaataac aaaaaaacgc
     1621 tatttcgcgt ttgaactgta cctcaaaagt tagacagaaa aagtctaatt tttagggtgt
     1681 ttttgttaag aaaagacctt caatcattta agaaaaatgt acatctagat ttatagccct
     1741 ctaaaactct tgcttttttt gtagaagata cgaaaaaagc catgaaacca aggcttttcc
     1801 tgttttatgt agatgtttac acatgaaaat tgaagtattt tcaaaagcta attggagtga
     1861 ttttattcat gatgggataa atgatctggc caaaaatgat tgagaaatag ttgcttaaaa
     1921 agcatttgca tgatactata tattgaaagg aggtgataat atggtagaac aaagaaaatc
     1981 aattaccatg aaagatgttg ctttagaagc aggagttagt gttggaactg tttcacgtgt
     2041 aattaataaa gaaaaaggca ttaaagaagt aactttaagg aaagttcaaa gggctataaa
     2101 aacattggac tatatccctg ataattatgc tcgaggtatg aaaaagaata gaacagaaac
     2161 tatcgctttg attattccga gcatatggca tccatttttt tcggagtttg cctatcatgt
     2221 tgagaaagag ttattaaaat ataattataa attatttatt tgtaacgctg atagcgattc
     2281 acaaaatgaa atcgaatata tcgaaatgtt gaaacaaaat aaagtggatg gaattattgg
     2341 tattacatat tctgatattg acaaatatat tttatctgat ttaccgtttg taagtataga
     2401 tagacatttt tcggaaaatg tatgctatgt tacttcagca aattacaaag gaggacaaac
     2461 tgcagcagag gaactggtta gaagggataa taagcacctt gcttttattg gttcaattaa
     2521 tatgtatgag aatgagacta tactcagaag taaaggattc agagaaaaag ctaaggaatt
     2581 aggagtagat gtctataatt ttgaaatgaa agaaccttta ataaatcctg aagatcagat
     2641 aagaattttt ttgcaatctc ataaagaaat tgatggtata tttacaataa atgatatgat
     2701 ggctctgaga gttattagtg ttatgaaaga actggggaaa atagcacctg atgattacca
     2761 gataattggg tttgatggtt taaaaacttc tgtcgatcaa tcttatctag tgtctacaat
     2821 agttcaagat attggtatga tggcaaaaga atctgttgca atgatactga aaatgattaa
     2881 gcgagaagag attctagaaa aacgaatcgt aattcccaca tatttttggg gagataaaac
     2941 aacaaaaaaa atttaaaaag cgcttgacaa ttaatgaaag cgctgttata atataatcaa
     3001 caaatgaaac gtttcaaaaa ataggctaga agcttgttta aaaaataaat aaaaaatgaa
     3061 acgtttcaaa aaggaggaaa atgatgcgta aaggtacaat gttatctgtt gttgcggggc
     3121 tatcgttagc tatacttgca ggttgtagtg gaggaactaa ctcaaaacaa gcgagttctt
     3181 caaatgatat taaagtctgg gtacaattct ctgacgaaac aaatgaagga aaagcttggc
     3241 aaaaagttgt tgataacttt aatcaatcag gaaaaagtaa gtacaaggtt gtaactgagt
     3301 atattcctcg tagtggtagt ggtggaggat atgaagacaa ggttaatgct gctattacga
     3361 ctaatagtct tccagatgtt attacccttg atggaccaaa tacggcagct tatgcaaaat
     3421 ctaaagtaat tactccgcta gatgattatc taaaagataa taatatggat gatgttttgg
     3481 acagtattaa acaacaggga acttataatg ggaaattcta tgcatttgga ttttctgaat
     3541 cgaatgttgg tgtttattac aataaaaaaa tgttcaaaga ggccggaatt gcggaatcag
     3601 agcttccaac gctagaaaag ccatggacat gggacgaatt taatacgatt gctaaaaaat
     3661 tgaaagatca ttataacaaa ccagcaattg attttcgtat taattcaaac gatgaaatgt
     3721 tgccatatgc ttatatgccg ttgatttggt caaataatgg ttctgtcgta aacgaagatg
     3781 gaacaaaggc agaaggatac ttcaactcca aagagagtgt agaagcagtt caattcattc
     3841 aaaatcttgt aaaagaaggc tatacaactg ttagtccagt cgaaaaaggt tttgaaacag
     3901 gagagtatcc aatgcttttg agtggttctt ggacgattgc tgacatgaat acaaactaca
     3961 aagatattga ttttggtatc cttccttacc cagtatctag taagacgaaa aaattagtat
     4021 ccccttcagg tagttggcaa ttagctgtga cgacaaaatc tgataaaaaa gaggccgcat
     4081 ctgaatttgt taaatttgct acaaatacag aatcaagtga aattattagc ttggggaatt
     4141 cggtccttcc aattcgtaaa tctacaattg aaaatatcaa agataaagta tccgaaccaa
     4201 tgcgtttctt gatggaacaa aattcaaaaa cagctcatgc tcgcccagtt gttgtagcat
     4261 atcctcaggt ttctcgagct tttcaacaag caatgcaaga tattagctat tatgaagaaa
     4321 atccaaatgt gcaaaaagtc ttggatactc gtacaaaaga aatgcaaact gctattgatc
     4381 agtcactcaa ataatctgta aaaaagaaag tggaaggaaa atgtttggaa ctaccttccc
     4441 ctttcttgtt aatagcctag tttgatatat atgattaaag gagatggata tgagtaaaga
     4501 aactgttgtg gatacaaaaa gacgtgaaaa aataaaggat aacatattag gttatagttt
     4561 tttagctcca gctttaattt tattaggtat ctttttagta attccagttg gaatggtcat
     4621 ttattacgct tttacagatt attatttgtt gacaccagat gaaagaaaat ttgttggact
     4681 tgaaaatttt attcgattaa caaaagatcc gattttctta aagagcttct tgaatacttt
     4741 gaaatttgtt gtttggatta ttccagttca attgggagcg gccttaggaa tggctttgat
     4801 tgtcaataaa aagcgaaaag gaaatatgtt ttttaaagtg gctttttttg ctccagttgt
     4861 catgtccttg gtagttattt ctattctttg gctttatctt ttaaatccaa atagtggctt
     4921 attaaatgct attttaaata aggtaggtat tgcttctcaa cctttcttga ctagtcctaa
     4981 acaagctatg tatgcaattg tttttgtttc tgcttggcag ggtgcaggtt atcagatgct
     5041 tttattttta ggaggaatgc agaatattcc acaagatgtc tatgaagctg ctgaattaga
     5101 tggattctca aaatgggctc agtttcgtta tatcacgatg cccctactaa aaccaacagc
     5161 cctgtttgta ttgttaacaa ctttaatttc tgcttttaag ctgattgtac aaccaatggt
     5221 tatgacgcaa ggtgggccat taaactcaac aattactatg gtgtactata tttatcaaca
     5281 aggttttact gatcgtcttg taggatattc tagctcaatc gctttagtat ttacaacgtt
     5341 aattggtatg attagcttag tacaacgtcg cgttctgaag gaggatgact aagatgaaac
     5401 gatttaaacc aacaacaatc ttagaatata ttctgttaac tttattggca ggactcttcc
     5461 tttttcctat ggtttggatg gtagtttcag ctatgaagcc agaagcggat gtttataaaa
     5521 atttgactag ttggcaagcc ttcttaccaa gtttaaatcc cgctgattgg tttaaacctt
     5581 atcaggaagt atttgagcgc tttagtattt ttacatatct agggaacagc attttttatg
     5641 ctggaacgtt tgctattggt tctattattg tcaatgcact ggccggtttc gcttttgcaa
     5701 aagtcaattt ttcaggtaag aaaattttat ttggtttctt acttgcttta cttattattc
     5761 cagtggaaac tgtgttaatt ccacaattta ccattatcaa ttccttaggt ttggtcaata
     5821 atcgtcttgc agttattatt ccaggattgg cgagtgtatt taatatttac ttatttagga
     5881 atttctttat tgcaattcca gaagagattc ttgagtctgc aaagatggac ggagcaagta
     5941 tcgttagaat tttctttaga attatgcttc caatgtctaa acctgcagtt gctacagttg
     6001 gtgttttatc atttatttca agctggaatg attatatctg gccattgatg gttttgacag
     6061 attcttctaa attttcaatg caggttgcaa ttacaactat taatacaact caaccagttt
     6121 atatcaatca ggtaatggct gtattaacta tttcaacgat tccgttgatt ttggtctatg
     6181 tagtcgctca gaaatatatt gttcaaggtc tgggtggttc cggaactgga ataaaatagg
     6241 atatacttat gataaataaa acatttacag tagaaaaagc caatcgtttt atagcagaaa
     6301 ataaacatct cgttaatact caatataagc ctgaagaaca tttttcagct gagattggtt
     6361 ggatcaatga tccaaatgga tttgtttatt ttcgtggaga ataccatctc ttttatcaat
     6421 tctatccata tgatagtgtt tggggaccta tgcactgggg acatgctaaa agtaaggact
     6481 tggtgacttg ggagcacttg ccagtggcac ttgctcctga tcaagattat gaccgaaatg
     6541 gttgtttctc aggctctgcc attgtcaagg atgatcgcct ctggctcatg tacactgggc
     6601 atatcgaaga aaaaaccggt gtccgccaag tgcaaaatat ggcattttca gacgacggga
     6661 ttcactttga aaagattgaa caaaatccag ttgcaacagg agcagacttg ccagatgagt
     6721 tgattgctgc tgatttccgt gatccaaaac tctttgaaaa agatggacgc tattactccg
     6781 tagtagctac caaacacaag gataatgtgg gctgtatcgt tctactaggg tccgataacc
     6841 tagtagaatg gcagttcgaa tccatctttt taaaagggag agaacaccaa ggctttatgt
     6901 gggaatgccc agattacttc gagttagatg ggaaagattg ccttattatg tcacccatgc
     6961 gttatcagcg tgagggagac tcatatcata acatcaactc atcgcttttg ttcacgggta
     7021 aggtagattg gggagaaaaa cgttttatcc cagaatcagt tcaagaaatc gatcatggcc
     7081 aagacttcta tgcgcctcaa acattgttgg acgatcaaaa tcgtcgtatc ctgattgctt
     7141 ggatgcagac atgggggcgt acccttccaa cccatgacca agaacacaaa tgggcatgtg
     7201 ccatgacttt acctagaatt ctaagattgg aagatggcaa actaagacaa ttccctgtta
     7261 aaaaaggcca atatcaaatc caaatagata aagattgtca ttaccactta ggaaatgata
     7321 cagattatct tgaatttggt tatgacagta atgcgcagca agtttacatt gatcgtagcc
     7381 atcttattca aaaaattcta ggtgaagaag aacaggacac tagtcgacgg tatgtagata
     7441 ttgaagctaa agaattggaa gttgttctag ataaaaattc catcgagatt tttgtcaatc
     7501 aaggtgaagc aagcttgact gcaacttatt acttaacggt gccagctgag ctatcacgaa
     7561 ttgattaaaa attaagttat ttctcctaaa gaaaaagttc tctttctaaa atagtggaaa
     7621 gaggactttt tgtgttttgg gtatataagc ttagtttatg gtatttgtaa aattggtgtt
     7681 ggattatgat ttaagctagt tttctaaaga atttgaaaaa aattttattt aagcaaaaaa
     7741 accttggttc caaggctttt cctgttgtat ttagatgccc cctacaggga tcgaacctgt
     7801 gacccacgga ttaagagtcc gctgctctgc cagctgagct aaggaggcaa agaaaaagct
     7861 gtattggtgc cgaaacttca cggtttgtat tgaacccgcg caattaagca ggtgggcaac
     7921 tcgctctaac tgaagctgtt tccgtgtgag acggcctaca tgctgttaga agacttttgt
     7981 ttccctaata atacaaaaaa tagtcggtca acacttaagt gtgaagtcgt acaccacagc
     8041 gtttctatac ttatatgata ccactttttc aaaaaaaatc aagaggaaag tgcaattttt
     8101 tgaaaaggat ttcagatttt tcgcaaacga tcgatagctt cctttctttg agccaaagcc
     8161 cgttcatatt taccagtatc ttctgcttgg aaatagtgat gattacgaat tttttctggc
     8221 agatagtctt gcttgaccca atttccagga tagttgtgtg gatagagata gtcttgggca
     8281 ttccccagtt ccttgcttcc actgtagtgc ccatcacgca ggtgtcgcgg aataggcaag
     8341 tgccctgatg ttttgaggtc agcaagtgcc ttatccatag ctacataggc tgagttggat
     8401 tttggagaaa gggccaaatc aatcacgaca ttggcaatga gaatgcgggc ttcggggaaa
     8461 ccaatccttt gtgcagcatc cagagcagtc acggtatgaa tctgggcctc aggattggct
     8521 aagccgatat cttcataggc gataacagtc aagcgacgag cgagactagg cagatcccca
     8581 gcctcaatca agcgggcagt atagtggaga ctggcatcca catctgagcc acgaatagac
     8641 ttttgcaggg cagatagaac atcatagtga ccgtctccat ccttgtccat agtgatgtag
     8701 cttctctgaa gactattttc catgatgtct aaggtgatgt ggcgaatgcc ctcgtcattc
     8761 tcaggggtag atagaacagc caagtccagt gagttaaagg cagagcgaag gtctccgttt
     8821 gtagaggttg cgatgaaatc cagcgcatcc tcatctagtt ctattggaaa atcaaaacca
     8881 cgttcagggt tacttagagc tatctgcagg gcctctttga cgtcttggtt agacagaggt
     8941 tccaactcga aaatttgaac tcggctacga atggcaggag tgacagagaa gaaaggattt
     9001 tcagtcgttg ctccaatcat gatgaccagt ccactttcca agagaggcaa gaggaagtct
     9061 tgcttggtct tatctagtcg atgaatttcg tctagcaata ggacgagacc accagaaaat
     9121 ttagcttctt ccgagatttc ttgcagtcgc tttttactat caactgtcgc attgaaagtt
     9181 cgaaaggcat acttggtcgt tccagcgatg gcagaggcaa tactggtttt gccgattcca
     9241 ggagggccat atagaatcat ggaggacagg cggttggctt ccaccatgcg gcggatgatt
     9301 tttccaggtc cgaccagatg ctcctgaccg atgacctggt cgatggtttt agggcgcatg
     9361 cgaagcgcga gattgtctgg catagcagtc ctttctaacg tggatttgag tagaatgact
     9421 catctactat atcttactat gtccattcta gcctattagt tgtgatattt caatggtttt
     9481 gtaataatca gggagtgaga tagaatcttt tttactattt aatttaatga ggagaggtat
     9541 cagcatgaga gatttgattt tccttaaagc tcaaaatagg atagttgagc ctaaagaagt
     9601 ggaagtgctg acttgagaaa taatattgct gaatgtcatt gtatgaacta taatgaaaag
     9661 agaggtgata agcaatggaa atgatggacc aagtcaggaa attttgattt ttacaaaaca
     9721 aattcgtcat tggattctta gtgatcaagt tatttcaggg aaaagaaaac ttttcttccg
     9781 ggaagatact ccaaaagaga ttttagattt gtacgaaaac attaaatcta aacttgattt
     9841 tgcttaccaa gaagtttatt ctaataatgg gttgaaaaaa tatgaaaaat agaataattg
     9901 atgtttttga agtagtaaat cgcatccttg taataactgt tgaaaatcct aatttcgaag
     9961 acttgagagt taatcatact caatgaaaat caaagagcaa actaggaaac tagccgcagg
    10021 ctgtacttga gtacggtaag gcgacgttga cgtggtttga atttgatttt cgaagagtat
    10081 cagtttgtaa aaattggaga taaaaaatat agagtacgca gtggtcctat gattcattca
    10141 actccgcctc aatctgtctt agatcgagat acttttacaa ttgactatac agatgatgaa
    10201 ttgctcgata aagaagcagt ttttactact cattagaaaa ataggtctga ataaattggt
    10261 ttcccttaga accacatcta ggggctttta tgtataatag aataataagt atcactcctt
    10321 tacaatacgt gtaacacgtg ttataatggt gttgtcagga ggtaaaagcg ctatacctat
    10381 gacacaaaaa gagatggtta aactccttac ggcccatggt tggataaaga ctagaggcgg
    10441 taaaggctcc catattaaaa tggagaagca aggggaaaga cctatcacaa tccttcatgg
    10501 tgagctgaat aagtacactg aaagagggat aggaaagcaa gctgggttgt aaaattcagt
    10561 ccctgctttt tgatatggag gtagtgaaat gttagttacg tatccagctt tattttatta
    10621 tgacgacaca gacggaacag aagcgactta ttttgtccat ttcccagatt ttgaatactc
    10681 agctacacaa ggagagggga tttctgaggc tttggctatg gggtcggagt ggctagggat
    10741 aactgttgca gatttgatag agagtgatgg agagttacct cagccatcag atatcaatag
    10801 cttgtcttta attgataatg atccttttaa agatgatgaa gatttcgtgt caacctatga
    10861 ccttgataaa tcttttattt ctatggtatc tgttgatgta tcagaatacc taggaagtca
    10921 ggaacccatt aaaaagactt tgaccatacc aaaatgggca gataagttgg gacgagaaat
    10981 gggacttaac ttttctcaga ctttgacaga cgctattgca gataagaaag ttcaagccta
    11041 agattaagcg tggtatcgtg gtaatagtca ataaaaaagc acgtccactt gtgctagtta
    11101 ctcgcctatt gattttatag atttattagc cctttcgcgg gctttttata ttgattttta
    11161 tgagaaatga agaaatcaga cttttaagaa aatcactgat accaagggtt taaatgaggt
    11221 aatatggtat aattaggaca taaaataatt ttgtggtaag atggtagtat ctattttagc
    11281 atatttccga gcaatcgggg cgattaaaga gtcgcataga aagaggacaa aatggcaaca
    11341 tacggatttt tagatgtttt agaggaagag ttggacaaga actttccctt tgactttgag
    11401 attagctggg acaaacgcaa ccatgcggta gaagtgagtt ttctattgga agcacaaaat
    11461 gctgcaggtg tggagatggt ggatgaagac ggagaggttt catcagatga cattcttttt
    11521 gaggaagcgg tccttttcta caatcctgct aagtcaaccg tcaatgcaga agactattta
    11581 acggttatcc cttatctacc taaaaaagga ttttctcgcg agtttttagc ttattttgcc
    11641 cttttcctca aagatactgc ggaagttggg ctagataccc tcatggactt tttggaagat
    11701 ccagaagcag aggaatttgt tatggaatgg aatcaagaag tctttgaaga aggaaaagtt
    11761 ggcttggaag agggagaatt ttatccttat ccaagatact aggagttggt tggaggtgtt
    11821 atgaagaaaa ttgggactta tttggtttat gtgctagctg ttgtctttat tatgctagct
    11881 tttgcttgtg gaacaatcgc atttgcagag ttggggtatt ccgcagtttt agcctttact
    11941 tttggttatg ccttcgctct tctaagcatg tatttaatct ttattcttca tgagctgggt
    12001 catgcttttt gtggctactt gacaggctat cggctggtgg cttttggatt aggacatttt
    12061 attttgacca aaaagtcagg caggtttcat cttagtagaa cagccattct gaaaaatgtt
    12121 ggtgctcaat acattggttt aaaagaggat gaaagcgatc aaagaatcat cctgatgctt
    12181 tcaggaggct tgatggttca tctcggcttg atattattgg cgatagtgtt tggatttttg
    12241 acaagaagct ggtattttgc agggacttgg atttttctta atttatcttt cttcctaaat
    12301 aacattttgc cagtcgacat caccgatgga gcaaaaattt gggaattact acaacaccct
    12361 gaaaatacca aatacgccta cctggtattg agacattctg ctcaaacttt gctggctcct
    12421 caagaatatg atttgaaaga ctttgttcaa gcagttcctg aggatgcacg agggagtttt
    12481 gcggatggtg tattaggtat gcaaggtgaa gtttccattt tggaagggaa ggaagaagta
    12541 gctaaacagc agtttcaagc attacttgag aggacaacta ctccgatgat gcagacagtt
    12601 gcccaactat ccctgcttca tatcgccttg ttggaaggag attttgagca agcggagcaa
    12661 tatgctagta ttcgacaggt caaatcgttt ctgtctctca aactgtccaa tcttcaagtg
    12721 atacaagcct ggtatcaatt aaaggtgaag aaagatgtag atcgaactcg taaggctctc
    12781 aagattgcta gacagaaaat ggatagtagt cgaatgttga gggacgagaa acgctattat
    12841 gaaaacttgt tagcagagct ggaaaaggaa atgttagaag gaactgaaga tggaactaga
    12901 aatttctgat ttccaaggtt gcaagattgc cctcttttgt ggggataagc tactgacgat
    12961 tttgcgtgat gacaaggacg atatcccttg tcccaacatg tgggagttgc caggtggtgg
    13021 ccgtgaaggg gacgaaagcc cttttgaatg cgctcggcgt gaagtttatg aggaactggg
    13081 aattcattta gatgaagact gtctgctttg gagtaaggtt tacccaagta tgctttttgc
    13141 agacaaacag tccgtttttc tagttggtca gttgacacag aaccagtttg acagtatcgt
    13201 atttggagat gaaggacagg gctatcagct gatgaatgtt gaggaatttc ttagttccag
    13261 tcaagttgtc cctcagttgc aagagagatt aaaagattat ttaaaagtaa gtgattagaa
    13321 aggatggttc agttaaattt ctaaactgaa cccgccctaa acactgtgcc aaaaagataa
    13381 acttctctta gacacaagcg tcttcagaga atttcctatt ttggttttgt gttttacggg
    13441 cttggtatct taataatgga aacatggcaa gagttaaaag ttacagtgaa gcgtgaggga
    13501 gaggagttgg tttccaatct cttgattgag ctgggagcgc aaggagtcgc aatcgaagac
    13561 agtatggact atgtgggaaa tgttgaccgc tttggcgaga ttttcccaga ggtggagcag
    13621 caagaagaaa tcgttgtgac agcctactac cctgacacgg tggatgtgac agtggttgag
    13681 gcggacttgc aggctcgcat atcagaattg acggatttta tggatttggg agagttaaaa
    13741 ataggtacga ctgccttggc tgaggaagac tgggcagaca acaggaagaa atactatgag
    13801 ccagctcgta tcactcatga tttgaccatc gttccctctt ggacagacta tgaggcgact
    13861 gcgggagaaa aaattatcaa gctggatcct ggcatggctt ttggtactgg aacccaccca
    13921 actaccaaga tgagcctttt tgccttggaa caggttcttc gtggtggcga aacggtgcta
    13981 gatgtgggga ctggttcagg ggttctctct attgccagct cgcttctggg ggctaaagaa
    14041 attttcgcct atgacctgga tgatgtggcg gttcgtgtgg ctcaggaaaa tattgagctc
    14101 aaccctggca tggaaaatat ccatgtagcg gctggagatt tgcttaaggg tgtggaaatt
    14161 gaggcagatg tgattgtggc taatatcttg gcggatatcc tcattcatct aatagacgat
    14221 gcctatcgtt tggttaagga cgaaggctac ctcatcatga gtggcattat caaggacaag
    14281 tgggacatgg tgcgccagtc ggctgagtca gctggatttt tcctcgaaac tcacatggtt
    14341 caaggggaat ggaatacctg tgtctttaag aaaaccaagg atatctctgg tgtgattgga
    14401 ggctagcatg cagcagtatt ttgtaaaagg cagtgctatc tctcctgtca ctatcgagga
    14461 caaggaaacc agtaagcata tgtttcaggt tatgcgcttg aaagaagagg atgaggttac
    14521 cttagtcttt gatgatggca tcaagcgctt ggcgcgcgtg ctggatatgg aaaatcgtca
    14581 gtttgagttg gtccaagaat taaatgacaa tgtggaacta ccagtccaag tgaccatcgc
    14641 atctggattt cccaagggag acaagctgga gttcatcgct caaaaagtaa ccgaactggg
    14701 tgctagccaa atctgggcct ttcctgcaga ctggtcagtt gccaagtggg atggcaagaa
    14761 attgggtaaa aaagttgaaa aactagaaaa aattgccctt ggagcagccg agcaaagcaa
    14821 gcgtaatatt gtaccaagta ttaaactttt cgagaaaaaa gcagattttc tagctcagct
    14881 ggaccagttt gactttatca tagtagccta tgaagaatca gctaaagaag gagaagccgc
    14941 tgcgcttctg caagcagtca ctggtcttga aacaggagcc aaactgctct ttatctttgg
    15001 tccagaaggc ggtctctcac ctgcagaaat cgaaagtttt gaagctaagg gagccgtttt
    15061 ggtaggccta ggtcctcgta ttttgcgagc agaaacagca ccgctttacg ccttatcagc
    15121 ccttagtgtt ttagtagaat tagagaaata agaggaagaa aatggaacaa aaacaccgtt
    15181 cagaatttcc agagaaggaa ctctgggact taacagccct ataccaagac cgtgaggatt
    15241 tcttgcgtgc aatcgagaaa gctcgtgaag acatcaacca gtttagccgt gattacaagg
    15301 gcaatcttca cacttttgag gatttcgaga aggcctttgc ggaattagaa cagatctaca
    15361 ttcagatgag ccatattggc aactatgctt ttatgcctca gacgacggac tatagcaatg
    15421 acgaatttgc caatattgcc caagctggga tggaatttga aacagatgcc agcgtagcct
    15481 tgaccttctt tgacgatgcc ttggtggtag cagacgagga agtcttggac cgtttgggtg
    15541 aattgcctca cttgacggca gctattcgtc aggccaaaat caaaaaagcc cactatctag
    15601 gggctgatgt ggagaaggcc ttgaccaatc tcggtgaagt tttctatagt ccgcaggaca
    15661 tttatactaa gatgcgagct ggggattttg aaatggctga ctttgaagcc catggcaaga
    15721 cctataaaaa cagctttgtc acctatgaga atttctacca aaatcacgag gatgctgagg
    15781 ttcgtgagaa atccttccgt tccttctcag agggacttcg taagcaccaa aatacggctg
    15841 cagcagccta tctggctcag gtcaagtctg aaaaactctt ggctgatatg aagggatacg
    15901 actctgtatt tgattatctt ctagctgaac aagaagtgga ccgtgtcatg tttgaccgcc
    15961 agattgacct catcatgaag gactttgcac cagtcgctca gagatacctc aagcatgttg
    16021 ccaaggtaaa tggtcttgaa aagatgacct ttgcagactg gaaattggac ttggacagcg
    16081 ccctgaatcc tgaagtgact attgacgatg cctatgattt ggtcatgaag tcggtagaac
    16141 ctttggggca agaatattgt caggaagttg ctcgttacca agaagagcgc tgggtggact
    16201 ttgctgctaa cagtggcaag gattccggtg gttatgcggc ggacccatat cgcgtacacc
    16261 cttatgtact aatgagctgg acaggccgtt tgagcgatgt ctataccttg attcatgaaa
    16321 tcgggcattc tggtcaattc atcttttcag acaatcacca aagctatttc aacgcccaca
    16381 tgtcgaccta ttatgttgaa gcaccgtcaa ccttcaatga attgctactc agtgattact
    16441 tagagaacca gtctaatgac ccacgtcaaa aacgcttcgc tctggctcat cgcttgacag
    16501 acacctactt ccataatttc atcacccacc tcttggaagc cgccttccag cgtaaggtgt
    16561 atacattgat tgaagaaggg gagacctttg gagcaagcaa gctcaacagc attatgaagg
    16621 aagttttgac agatttttgg ggcgatgcca ttgaaatcga cgacgatgca gctttgactt
    16681 ggatgcgcca agctcactac tatatgggct tgtatagtta cacttactct gctggtcttg
    16741 tcatctcgac agctggttac cttcatctta aaaactcaga aactggagct gaagactggc
    16801 tcaatctcct caaatcaggt ggtagcaaga caccacttga gtcagccatg attatcggtg
    16861 ctgatatttc aacagacaaa ccactccgtg ataccatcca attcttgtct gacacagttg
    16921 accagattat cgcctacagt gctgagttgg gggagtaagg ggaatagaaa aattttctag
    16981 aatgatgttt atgggcttgt taagcgtcat tctatttgtc ttagttgtag ctattttcaa
    17041 aaatgtcgtt gaaggtcgtg atgttaaatt tgaacttact gtagaactgg tattgatagt
    17101 tatttacgtc attttatatc agattgtatt taattgagaa ttcaatattg agtcatttaa
    17161 gactgccttt ttgaacagtt gctagaaatg tagaggtatt gaccatgtat caagatttac
    17221 taagaaaaat agcagaagaa aaaccaaatt ataaccagga agaaatccag tggttgtttg
    17281 atcatttggg aaatccttct ccagaaattc gtgatgacct ttcaaatcaa ggcttgcact
    17341 atctttcaaa agaaaaggat acaacagatt tctcaagtca gtatggttgg gttcatgctt
    17401 ttgcacatgg agccgattta ctgacagagg tggtttgtca tccagacttt cctataaaca
    17461 gaattcatga agtatttgac atacttggcc aactatttaa aagaatgtcg attcgcttta
    17521 cagatgatga ggattggcgt ttagcaagag tgatctatga acctatttta caagggaagt
    17581 tggagcaaga acaagtagct tcttggataa aaactgttga ttttccgata gaagaaaggg
    17641 aggattttta caaattttcc aactttagat cctgtctggt ggaagtctat gtccaacttg
    17701 accagagaaa tagtttacaa gatgacctga aagaagctat ccagtctttt caatactaga
    17761 gattagctaa tagttctatg attttcaaaa aatttccaat caattttctt gaaacccttt
    17821 cctacttttg atagactagt atctagtttt tgaaaaaagg agaaagaaaa tgaaaaaatt
    17881 tgttgctgag ttaatcggta cgttcatgct tgtgttcgtt gggacaggag ctgttgtttt
    17941 tggaaatggt cttgatggcc ttggtcacct tggaatcgcc tttgcctttg gtttggcaat
    18001 cgtggtggca gcctactcaa tcggaactgt ttcaggtgct cacttgaacc cggctgtttc
    18061 gattgctatg tttgtaaaca aacgtttgtc atcttcagaa cttgtaaact acatccttgg
    18121 tcaggttgtt ggagctttca tcgcttctgg cgcagtcttc ttcctcttgg gtaattcagg
    18181 tatgtcaact gctagtcttg gtgaaaatgc cttggcaaac ggtgtcactg tctttggtgg
    18241 tttcttgttt gaagtcatcg caactttctt gtttgtcttg gttatcatga ctgtgacttc
    18301 agaaagcaag ggcaatggcg cgattgctgg tttggtaatc ggtttgtcat tgatggcgat
    18361 gattcttgtc ggattgaaga ttactggact ttcagtaaac ccagctcgta gcttggcacc
    18421 agctgtcttg gtaggcggcg cagcccttca acaagtttgg attttcatcc ttgcaccaat
    18481 cgctggtgga gttcttgcag cccttgttgc aaaaaatttc cttggaacag aagaataatt
    18541 gaaactcaaa aagccttgct cctcatcttg aggaacaggg ctttttcgta tgatactctt
    18601 cgaaaatctc ttcaaaccac gtcagcttca ccttgccgta ggtatggtta ctgacttcgt
    18661 cagttctatc cacaacctca aaacagtgtt ttgagcaacc tgcggctaac ttcctagttt
    18721 gctctttgat tttcattgag tatgacttta gcggttgtca attttctctg gataaaggtc
    18781 gtgttggaag aggcgttgtt ctgccaagcc ctcatactta gttccttgct taccgtagtt
    18841 gtagtagggg tcgattgaaa tgccaccgcg cggagtgaat tttccccaga cttctaaata
    18901 gcgagggtct agcaagttga ccaagtcttt cccgatggtg ttgatacagt tttcgtggaa
    18961 atctccgtgg tttcggtagc taaatagata tagtttgagg gattttgact cgacacagag
    19021 cttgtcagga atgtaggaaa tatgaatcgt cgcaaagtct ggctgagcag tgatttgtcc
    19081 cagcagagac atatcgagga tatggtgacg aatgccctgt tccttagcga tttctctagt
    19141 aatttgaatt tcgaggtgat gacgttggcc gtaggcaaag gtgacagctt cgactgtttc
    19201 atagtgttgc atggcccaga aaaggcaggt tgttgaatct tgaccaccac taaagacgac
    19261 caaggctgat tgatgtttca tagtactcct tccaaaatgg gaaatgttca gagcacgcaa
    19321 aaagctccca ttagggagct aaaaaatacc aaatcgagtt tttttagcga tggcatatcc
    19381 caaacatcgt aatattctac ttatatagta aaatgaaata agaacaggac aaatcgatca
    19441 ggacagtcaa atcgatttct aacaatgttt tagaagtaga ggtgtactat tctagtttca
    19501 atctactata gtctagcata ttttttgaaa aatggcaaag gacaagaaaa aagagaccaa
    19561 agaaagtact tggtctctcg tttgattagc tcaattcagc aatgatggcc ttgatttgtt
    19621 ctgctgtgtg aacacctgca acttgtttga caacttggcc gtcttttttg aagagaagag
    19681 ttggaataga catgattcca aaagcacgag ctgtgtttgg attttcatca acgtccattt
    19741 taacgatttt caagacatct tctgaaagtt cttcagacaa tttgtccaag attggacctt
    19801 gcatacgaca tggaccacac caagttgccc agaagtctac taagaccaaa ccgtcttttg
    19861 tttcttgttc gaatgttgca tctgtaattg cttttgccat tgtatttctc ctttttttag
    19921 ttatattggc ttaaatcttg tttcatgaga tagaagaaga tatctccata agtcccatgg
    19981 tagtccaaat tatgaccctt gtaagttaat ttttggacag ggtagtagtc tgcgacgccg
    20041 ataaggcaag cttgttgcga acgttcaaag tcttcataag actcgaaagt cacagttctt
    20101 tcgttcttgc tggcatctat ataggtaatt tcaatcatat ttaaaactcc tttgtttaat
    20161 gctaacttta ttttactcct tataaaagag aatgtcaaga aaaatgattg cgcacgcaac
    20221 tttttttaaa aatcatctta aatcaagaaa tccaaacctg cttccaagct ttcttcgaca
    20281 gttttttgta gcgaggccag tgtcttttgc ccatcatttg tcaggcagat aaaactagag
    20341 cgtctatctt gatggcaaca catgcgactg agtagaccgc aatttttagc ttccaagcga
    20401 gccaccatcc tagaaactgc gctcgggctc agatgaagct tatctggcag gtcaatctgg
    20461 cgtagagatt tttcttcagc caagtccaga tagtagagca ggtagaactc tttcaaggtc
    20521 agactttgct cgctctgttg ggcaatggtc tcttccaaga gactttcaat ttctttctga
    20581 cgccgattga agtcaaacca tttttccaaa taggtcatag tgtctccttt ctttttagag
    20641 tcataatata gaagaaagtc cattaacggg cagtctctgc gtcacaagat gattgcgcat
    20701 gcaataatta tactactttt caagaatgct ggcaagctct gttttttagt ggttttattt
    20761 tttactgtcc atatttttgg taaaaatcaa cttttacttg gatgaaggtt ttggcttcac
    20821 gtaggagttg aagaagggtg gcgcgggttt caaactcttc tcttgtcttg ggcagactgc
    20881 ggttccggaa gacttccaga taactttcaa tttcatctag caaatcagaa gcaggattgg
    20941 tctggctcag ttgaccagca atttttgaaa agagctgcgc taaaatcagg ctttcactgg
    21001 cggcaaggtg acaagtgtta atctgttggg ccatgtttct caggatacga ctttgtcgtt
    21061 gtctcatctc aaagtagtgg atatggtagt cggtctggtg aaagaggtgg tcagagtgat
    21121 ccaaatagac cagtctgagg gcttctttca aaagcgtgtc taattctgct accagctgtg
    21181 ctcggttgcg tccgtctcct ctggataaat agtatttgaa gcgctggagg atatctttta
    21241 acttttcttc caccagcgtg tggtagtgct ggatttcctc ttctcgtgaa ggcatataga
    21301 gattaacaag caaggcaaat cctgtaccaa tagcaaagag aaggaattca ttgactagaa
    21361 ggtctggaga ggttgactct tgaaccaaga gatgactaac caaaaccgtg cttggtgtga
    21421 tgccaatttc ccagcccatc ttgtaggcta aaggaacgta gaaggccaga tagaggccga
    21481 gactccagat atgaaatccg ctcaagtgaa aagctagaac actgatagcc agagctagaa
    21541 gcatagaaaa aagacgattg cgagccagtt ttaaagtact tctacgcgta tcagataggc
    21601 tcaagagagc gataattcca gctgaaactg ctgacgaaag attgagaaaa taagcaagca
    21661 ggcaggcaag acaggtagct aagatgagct tggtcgtacg ttggctaata gacataagaa
    21721 tttcctaata agttagaata aaagcgtaaa agacaagaca tgagcaggct tgccttgatg
    21781 agttattttt tacgggttgc tgcgtattcg gcaacggcgg taaagaggac atctgtagaa
    21841 gagttaaggg atgtttcaca tgagtcttgg atgacaccaa tcacaaaacc aaccccaaca
    21901 acttgtatgg caatatcgtt agaaataccg aaaaggctac aagcaactgg gataagaagg
    21961 agggaacctc cggcaatacc tgaagcacca caggctgaga tagctgctac cacactgagg
    22021 acaaaggctg tggcaaagtc aacaggaatt ccaagagtgt taactgtaac aagggtcaaa
    22081 aggttaatgg taatcgctac tccagccata ttgatagtag aaccgagtgg gatagaaaca
    22141 gaataggtat ctgggttgag tccaaggtca tggcagagtt tcatgttgac aggaatgtta
    22201 gtcgcagaac tacgagtgaa aaaggctgtc acaccgctga cacggaggca gttccaaact
    22261 agagagtaag gattgcgtct cataaagaag aaggcaatca aagggttgac cacaggggca
    22321 acaaaaagca tagtcgttac taatagaacc aataaaatac cgtagttggc aaggcttccg
    22381 actcccttgt cagaaatggt tttaaaaaca agaccaagga ttccaaatgg agtcagattg
    22441 atgatccatt cgacaatttt agaagtcacg tcagcgatag tttttagcaa ttctttacta
    22501 tttttactgg cttctctcat agcgattcca aaaatgactg cccaagataa gattccaata
    22561 tagttagcag taagcagggc gttgactggg ctgtcaacca gtttgagcaa gaggttgctg
    22621 aggacctgcc caatcccatc tggtggtgca atttcagtat tggcactatt tagggtaatt
    22681 tcaataggga cgatgaaact tgctagtaca gctacaagag cagcggcgaa agtccctatc
    22741 ataggatata caagaaaaca acagttttca tattgctatc ttgtcccttt tgatgttggg
    22801 aaagggcatt ggcaacgagg gcaaagacta ggataggagc aacagctttt agaactccaa
    22861 cgaataaatc cccgagtagc ccaatccctg agagattagg aagggtcagt cctaggattc
    22921 cccataaagc ataccaatca agatacgctt gacaaggctt gccttattcc aagcatgaat
    22981 gattcttttc ataataatct cctttttgtg tagtgattat gattatagta taaatgatag
    23041 acaaaatcaa gaattttctg tctatttttt gaatatttat ggagaatgag actgatgaaa
    23101 atatggtata atgaaataaa ggagttttat atgcaaaaat ttattcaggc ttatattgaa
    23161 aagctagatg tgacaaccat tatcgagaat attctaacca aggtcatttc tcttttactg
    23221 cttttgattg tattttatat tgctaaaaaa atgcttcata ccatggtgca gagaattgtc
    23281 aaaccttctc taaaaatgtc tcgtcatgat gtcggacgcc aaaaaaccat ctcacgttta
    23341 ctagaaaatg tgtttaatta tacgctatat ttctttttac tctactgcat tttgtcgatt
    23401 ttaggtttgc cagtttctag tttgctggct ggagctggta ttgctggggt agcgattggt
    23461 atgggagccc aaggctttct atctgatgtc atcaatggct ttttcatcct ctttgaacgt
    23521 caactggatg tgggagatga ggtcgttctg acaaatggac cgattactgt atcgggtaag
    23581 gttgtcagtg tgggaattcg tacgacacag cttcgtagcg aggagcaagc ccttcacttt
    23641 gtccctaacc gaaatatcac agttgttagc aatttctcac gcacagacta gacctgttat
    23701 tttaagtaat ttgtggtaca atagagggag tttaataagg agaaaagatg gttttagaaa
    23761 agcagttggg caatggttgt acctggatag acctagacct aggaaagttg aataaactag
    23821 aagacctttc tgaaatttac ggtttggaca aggaaaccat tgaatacgca ctggatagaa
    23881 acgagcgcgc ccacatggac taccaccgtg aaagtgggac ggttaccttt atctataatg
    23941 tcctagacgt aaaaaaggac aaggcctact atgagacttt tcccatgacc tttatcgtcg
    24001 agcatcgtcg cctgattacc attagtaata ccaagaacgc ctatgtcatt gaacagatga
    24061 ctcgttatct ggagaaccat gacacgcttt cgatttataa gtttctcttt gccagtctgg
    24121 aaatcatcag caatgcctac tatcctgtca ttgagcagat ggacaagagt agggatgagg
    24181 tcaatgacct cttgcgccag cgaactacca agaaaaacct ctttgccctg tctgatttgg
    24241 agactggtat ggtttatctg acggcagctg ccaaacaaaa tcggattttg ttagagcata
    24301 ttcaaggtca tgccttgtat cgtagttttg atgagattga gagagaacag tttgatgatg
    24361 ccatgattga ggctcatcag ctggtatcca tgacagacct aatctctcag attttacagc
    24421 agctttcagc ctcttacaac aatattctaa acaataatct gaatgacaat ttgacaacct
    24481 tgactattat ttcagtcttg ctagctgttt tggcagtcgt gacaggcttt ttcggaatga
    24541 atgttccctt acctttaaca gatgagcccc atgcttggct ctatattagt ttggctagtg
    24601 caggtttgtg gattgtttta tccttgttac taaggaaaat tgcgaaaaaa agttaagaaa
    24661 aggagccaga atggcgattg aaaattatat accagatttt gctgtggaag cagtctatga
    24721 tctgacagtc ccaagcctgc aggcgcaggg aatcaaggct gttttggtcg atttggataa
    24781 taccctcatt gcttggaaca accctgatgg aacgccagag atgaagcaat ggctacatga
    24841 ccttcgggac gcgggtattg gcattatcgt agtgtcaaat aacaccaaaa aacgcgttca
    24901 acgagcagtt gagaaatttg ggattgatta cgtttactgg gccttgaagc ccttcacatt
    24961 tggtattgac cgtgctatga aggaattcca ctatgacaaa aaggaagtgg tcatggttgg
    25021 cgaccagctc atgacagata tacgagcagc ccaccgtgca gggattcggt caattttagt
    25081 caaacccttg gtccaacatg actcaatcaa aacgcagatt aaccgaactc gtgagcgtcg
    25141 tgttatgaga aaaatcactg aaaagtacgg accgattaca tataaaaaag gaatttagct
    25201 atggaagaaa ttctctgtat tggttgtgga gcaaccattc agacgacaga taaagctggt
    25261 cttggtttta ccccccagtc ggcacttgaa aaaggtttgg agactggcga agtctattgc
    25321 caacgctgtt tccgtctccg ccactacaat gaaatcacag atgtccagtt gacgaacgat
    25381 gatttcctca agctcttgca cgaggtggga gacagtgatg ccttagtggt caatgttatt
    25441 gatatctttg attttaatgg atctgtcatc ccaggtttac cacgtttcgt ctcgggcaat
    25501 gatgtcctct tggtaggaaa taaaaaagat atccttccta agtcagttaa gtctggtaag
    25561 attagccagt ggctcatgaa acgtgcccat gaagaaggtc ttcgtccagt cgatgtggtc
    25621 ctaacttcag cacaaaataa acatgccatt aaggaagtca ttgacaagat tgaacactac
    25681 cgtaagggcc gcgatgtcta tgtggtcggt gtgaccaacg ttggaaaatc aactctaatc
    25741 aatgctatta tccaagaaat cacgggtgat cagaatgtca tcactacttc acgcttccca
    25801 gggacaacct tggacaaaat agagattccg cttgacgacg gatcttatat ttacgatacg
    25861 ccgggaatta tccaccgcca ccagatggct cactacttga cggccaaaaa cctcaagtat
    25921 gtcagtccta aaaaggaaat caagcctaag acctatcagc ttaatcctga gcaaacccta
    25981 tttttaggtg gtttgggacg ctttgacttt atagcaggag aaaagcaagg atttactgct
    26041 ttctttgata atgaactcaa actccatcgt agcaagcttg aaggagctag tgctttctac
    26101 gataagcacc tgggaactct tctgacacca ccaaatagca aggaaaaaga agatttccca
    26161 aggctagtcc agcatgtctt taccattaaa gataagacag acctagtcat ctcaggccta
    26221 ggctggattc gtgtaacagg catagcaaaa gtcgccgtct gggcaccaga aggcgtcgcc
    26281 gtcgtcacac gaaaagcaat tatttaaaac aaaatcgctg agcgaacaaa gtgagctcat
    26341 atagcgatag ttattgccgt ggtgtgattg ccaatctgta gattggcaag gggcacaatt
    26401 gagaccaagg ctcaattgtg aggtcaggaa atccattttt caaagatgaa atctttggaa
    26461 aattagttcc gaccgtccct caccacctaa aataactatc caaaaacaag gaagaaaatt
    26521 atgtcattaa catcaaaaca acgtgccttc ctcaacagcc aggcacacac cctcaaacct
    26581 atcatccaaa tcgggaaaaa tggactcaac gaccaaatca aaaccagcgt ccgtcaagct
    26641 cttgatgcgc gtgaattaat caaggttact ctcttacaaa acacagatga aaacatccac
    26701 gaagtagctg aaattttgga agaagaaatc ggtgtggata cagtccaaaa aataggacgc
    26761 atcttgattt tgtttaaaca atctagcaag aaagaaaatc gcaagatttc taagaaagtc
    26821 aaagaaatct aagaccgaaa ctccaaataa ctgtttttat agagaaataa aggggactag
    26881 cctatgacaa tcgaactatt gactcccttt accaaggtag agttggagcc agaaatcaag
    26941 gagaaaaaac gcaaacaagt tgggatttta ggggggaatt ttaaccctgt tcacaatgcc
    27001 catctcattg ttgcggatca agtacggcaa cagttgggac tggatcaagt tcttctcatg
    27061 cctgaatacc aacctcctca cgttgataaa aaggaaacca tccctgaaca ccatcgtctc
    27121 aagatgcttg agttggcaat tgaggggatt gacggcctag tcattgaaac cattgagttg
    27181 gagcgcaagg gtatttccta cacctacgat accatgaaga ttttgacaga gaagaatcca
    27241 gatacggatt attactttat catcggtgcc gacatggttg actatctgcc taagtggtac
    27301 cgaattgatg aactggttga catggttcag tttgtggggg ttcagcgtcc acgctacaag
    27361 gcagggactt cctatccagt tatctgggtg gacgtaccgc tcatggatat ctcgtccagc
    27421 atggtgcggg acttcattgc ccaaggtcgg aaacccaact ttctcctacc tcagcctgtg
    27481 ctagactata tcgagaagga agggctctac tgatggccta tcaagactat atcaactgct
    27541 ctcgtgaggt tttgttggaa aaaatggcag agcttctacc tgagaaacgt ctaacccatt
    27601 gtctaggtgt ggagcgtgcc gctatggaat tagctcagcg ctttggtgta gatgttgaga
    27661 aagcaagtct agcaggtctt cttcatgact atgctaaaaa gttgtcagat caggaatttc
    27721 tagacttgat tgaccgttac cagctagacc ctgacctcaa aaactggggt aataatgtct
    27781 ggcatggtat ggtcggaatt tacaaaattc aggaagattt ggatttgcat gattcagaaa
    27841 tcctgcgagc cattgaagtc catacagtcg gtgctggtca aatgacagac ctagataagg
    27901 tcatctacgt cgcagactat atcgagcaca atcgagcctt tcctggagta gatgtggcgc
    27961 gtgagattgc aagtctatcg ctcaataagg cggtggccta cgaaacagct cgtaccgtgg
    28021 agtatctagc tcatcaggga ttccccatct atccccaaac ccttgaaacc tataacgcct
    28081 ttgtgcacta tttgaaagag aactaaatga agacggcttt tataatcatt gatgttcaga
    28141 atgttttagt agaaactggg ttccagacaa aaagtctatt gggaaaaatt tcttatttac
    28201 aaaaccaggc tagaagcaag aatattgaaa ttatctatgt tcaacatatt gagaactctg
    28261 aagctcaaac atcagaagat tggcagttat ctgcactttt aaatcgaaaa cctgctgaaa
    28321 aggtctttca gaagaagtat aacagtattt tcaaagaaac tggcttaaaa gaatacttgg
    28381 ataagcaggg gattgaaaaa ttagttttat gtggtatgca gacagaatat tgtgtggata
    28441 cctctgtcaa ggttgccttt gaatatggct atcagcttat tgttccagaa ggtgctgtca
    28501 caacctttga tggggatgac attccagcag aaacgattaa tgaattttat gaggacattt
    28561 gggaggagcg ctttgcagat gtcctagatt acaaacatat tttctaaaaa gaggattaaa
    28621 tgaacgaaaa agaattacta gaactagtcg tgaaagcggc tgatgagaaa cgtgcggagg
    28681 atatcctcgc acttgatgta caagatttga ctagtgtgac ggactacttt gtcatcacta
    28741 gctcaatgaa tagccgtcag ttggacgcta tcgcagctaa tatccgtgaa aaagtagctc
    28801 aagcgggctt taaaggtagc catgtcgaag gcgatgcagc tggaggctgg gtcttactgg
    28861 acctcggtgc tgttgtcgtg catatctttt cagaagaaat gcgtgcccat tataatctag
    28921 agaagctatg gcatgaggcg aattcagtag atatttcaga agctcttgct tagaagatga
    28981 actcagttgt agtcaactgg gtttctttgc ttattttaga aaaattgata gaaaggtaaa
    29041 gggctagtgg tgtttaccat ggagctctta gtatcatgat tatggcgact tatgaaacct
    29101 ttgcggcggt ctatgatgcg gtcatggacg atagtttata cgacaaatgg acaaattttt
    29161 ctctgcgtca tttgcctaag accaaggaga aaaagaaact cttggaactg gcttgtggga
    29221 caggaattca atcagtgcgc ttctctcagg ctggttttga tgtgactgga cttgacttga
    29281 gtgcggatat gttgaagatt gcggagaaga gagcaacttc agccaagcaa aagattgctt
    29341 ttatagaagg taatatgctg aatttgtcca aggcagggaa atacgatttt gtcacgtgtt
    29401 attcggactc tatctgctat atgcaggatg aggtggaagt aggggacgtc tttaaggacg
    29461 tgtacaatgc gctgaatgaa gagggagttt tcatctttga cgtgcactcg acctaccaga
    29521 cagatgaagt attccctggc tattcctacc atgaaaatgc ggaagatttt gccatgcttt
    29581 gggatacata tgaggacgaa gctcctcact ccatcgtgca tgagctgact ttctttatca
    29641 aggaggctga cggttccttt agtcgccacg atgaagtaca tgaggagagg acctatgaga
    29701 tcttgaccta tgatatctta ctggaacagg ctggattcaa gtccttcaaa ctctatgcag
    29761 actttgagga taaggaacca acagaaacta gcacccgttg gttttttgtg gcgcagaagt
    29821 aggagattag catgaccatc acaggtatta tcgcggagtt caatcctttt cataatggcc
    29881 ataaatacct gctggatcag gcagagggac tgaaaattgt tgccatgtca gggaatttca
    29941 tgcagcgtgg agagcctgct attgtggata agtggacaag ggcccagatg gcgctggaaa
    30001 atggcgcaga cttggtagtg gaattgccct ttttagtcag tgttcaggca gctgatttct
    30061 ttggtcaagg agctgtggat atcttggatc gcttgggcat tgatagtcta gtttttggga
    30121 cagaggaagt tcgagattat cagaaaatcg ctgacttata cacagagaaa ggtgctgaga
    30181 tggagaaatt tgtggaaaat ctgcctgatt ccctttccta tccccagaaa acccaatcta
    30241 tgtggaagga atttgctggt cttgattttt caggtaatac ccccaatcat gttcttgctc
    30301 tggcctatgc aaaggcggtt gcaggacgaa atatcaagct gcatccgatt cagcgtcagg
    30361 gggcaggtta ccattctgtg aataaagatg tggactttgc ctcggcgaca gccctccgtc
    30421 aacatcagaa ggaccaagat ttcttagaac gctttatgcc ttctgttgcc ctatttgagc
    30481 aggccagtaa ggtgatctgg gaagactatt ttcccttgct acgctatcaa atcttgtcaa
    30541 atcccgacct aaccaccatc tatcaggtca atcaagaaat ggctgtgcgc attaaagaag
    30601 ctattaaaac agcccagtct gtagaagagt tggtcgaggc ggttgcgacc aaacgttaca
    30661 ccaaggcgcg tgtcagacgc ctattgacct atattttggt gcaggctaga gaaaatgtct
    30721 tgccagaagc cattcatgtc cttggattta ctgaaaaagg caggcaacat ctcaagtctc
    30781 tgaaaggaca ggtcaatcta gtcagccgaa ttggcaaaga accttgggat gctatgaccc
    30841 aaaaagcaga ccagatttac caactaggaa agccaagtat agcagagcaa aattttggaa
    30901 gagtgccgat tagaatagaa acaagctaag tctaccgaaa ggtaggcttt ttgagatttt
    30961 ttacgaataa tactcttcga aaatcaaatt caaatcgcgt caacgtcgcc ttgccgtact
    31021 caagtacagc ctgcggctag tttcctagtt tgctctttga ttttcattga gtataaatag
    31081 atagaaaaga aaatcttgta caagttcctg acaatttctt tcttttcgtg tataatagtg
    31141 gaaaagagct aaaacgaggt atggttatgg aagcagtcct ttactcaaca ttccgaaatc
    31201 atttaaagga ctacatgaag aaggtaaatg atgaatttga gcctttgacg gtggtcaata
    31261 aaaatccaga tgaggacatt gtagttcttt caaagagcga atgggacagt attcaggaaa
    31321 ccctgagaat cgctcaaaat aaggagttat cagacaaggt tttgcgagga atggctcaag
    31381 tccgtgctgg aagcattcag gtccatgtga ttgaggagtg atgaatgcta ctcaagttta
    31441 cagaagatgc gtgggcagat tattgctact ggcaaaacca agataaaaaa acactaaaaa
    31501 gaatcaataa attaatcaag gatattcaac gcgatccatt tacaggaata ggaaaaccag
    31561 agccactcaa atatgattac caaggagcct ggtcacggcg tattgatgca gaaaatcgct
    31621 tgatttatat gatggatgga gatagcgtgg ctttcttgtc ctttaaagat cattactaag
    31681 tctattgaaa ggtaggcttt ttctcaagtc tctactaaac tactatttct aaaaaacaag
    31741 tttattttgg caaatgtgtt ccaaattccc caaaacatat tctattttag gtttgtgaaa
    31801 atcacttttt tatggtagaa tgtaaaagaa tgtatgtcat tcggaataac aataaaatga
    31861 ggtaaaaaca tggagatcat gtcgcttgcg attgctgttt ttgccgtcat cattggttta
    31921 gtcattggat atgtcagcat ctcagctaag atgaaatcat ctcaggaagc tgcagagttg
    31981 atgcttttaa atgctgaaca agaagcaact aatttacgtg gacaagctga gcgtgaagcg
    32041 gatttacttg ttaatgaagc caaacgtgaa agcaagtctc ttaaaaaaga agcactattg
    32101 gaggccaaag aagaagccag aaaataccgt gaagaagtgg acgctgaatt caaatcagaa
    32161 cgtcaagaac tcaaacaaat cgaaagtcgt ttgacagaga gagctactag ccttgaccgt
    32221 aaggacgaca atttgacgag taaagaacaa acacttgaac aaaaagaaca aagtatttct
    32281 gatagagcga aaaaccttga tgcgcgtgaa gagcaattag aggaagtcga aagacaaaaa
    32341 gaagcagaac tagagcgtat tggtgctctg tctcaggcag aagcacgaga tattatcttg
    32401 gctcagacag aggaaaactt gaccagggaa attgccagtc gcattcgcga agctgagcaa
    32461 gaggtcaagg aacgttctga caaaatggcc aaggacatct tggttcaagc tatgcaacgt
    32521 atcgctggtg aatatgtagc ggagtcaaca aactcaacag ttcatctgcc agacgatact
    32581 atgaagggac gcattattgg tcgtgaaggt cgtaacattc gtacctttga aagtttgaca
    32641 ggggtcgatg tgattatcga cgatacacca gaagtggtga ccttgtcagg atttgatccg
    32701 attcgtcgtg agattgcccg tatgactatg gaaatgttgc tcaaagatgg tcgtatacat
    32761 ccagctcgta tcgaagagtt ggttgagaaa aaccgtcaag agattgacaa taagattcgt
    32821 gaatacggtg aggctgctgc ctatgaaatt ggtgcgccaa accttcatcc agacttgatg
    32881 aagattatgg gacgtttgca gttccgtact tcatatggac aaaatgtttt gcgccattcg
    32941 attgaggttg ctaagttggc tggtatcatg gcgagcgaac ttggtgaaaa tgcggctctt
    33001 gcccgtcgtg ctggattcct tcacgatatc gggaaagcca ttgaccatga ggttgaaggt
    33061 agccacgttg aaatcggtat ggaattggcc cgtaagtaca aggaaccccc agttgtggtg
    33121 aatacgattg ctagtcacca cggagatgtt gaagctgaga gcgtgatagc agttatcgtc
    33181 gctgcagcag atgccttgag cgcagcccgt ccaggtgctc gtagtgagtc tcttgaaagc
    33241 tacatcaagc gtctccatga tttggaagaa attgctaacg gctttgaagg agtgcaaact
    33301 agctttgccc ttcaagcagg acgtgaaatt cgtatcatgg tcaatccagg aaaaatcaag
    33361 gacgacaaag tcacaatctt ggctcacaaa gttcgtaaga aaattgaaaa caatctcgat
    33421 tatccaggaa atatcaaggt aaccgtgatt cgcgagcttc gtgcagtaga ttatgctaaa
    33481 taaataagaa agagcagttg aaaaattact gcttttttgt tacactagat agaaagactg
    33541 taaaggataa tctggctttt tgccattatt ctagagaaat gttatttcac agattgacta
    33601 aaaggagaca caatggcaga ccgaggctta ctaatcgttt tttctggtcc ttcaggggtt
    33661 ggaaaaggaa cggttagaag agagattttt gagagttctg aaaaccaatt tcaatattct
    33721 gtatcgatga cgacacgcgc acaacgtcct ggagaagtgg acggtgttga ctatttcttc
    33781 cgtactcgtg aagaatttga agagctgatt cgtcaaggac agatgttgga atacgcagaa
    33841 tatgtcggca actactatgg aactcctctg acctatgtca atgaaacctt ggacaaggga
    33901 atcgatgttt tccttgaaat tgaagttcag ggtgctcttc aggtcaagaa aaaggttcca
    33961 gatgctgtct ttatcttcct gacaccacca gatttggatg aattgcaaga tcgcttggta
    34021 ggtcgtggaa cagatagtgc agaagtgatt gcccaacgaa tcgaaaaggc caaggaagaa
    34081 attgccctca tgcgtgagta tgattatgcg attgtcaacg atcaggtgcc cctagctgct
    34141 gaacgtgtca aatgtgtgat tgaagcagaa cacttctgtg tggatcgtgt cattggtcac
    34201 tatcaggaga tgttaccaaa atctccaact acccgataaa atttagaaaa taggtacaag
    34261 caaatgatgt taaaaccctc tattgatacc ttgctcgaca aggttccttc aaaatattca
    34321 ctcgtaatct tggaagcaaa acgtgcccac gaattggaag caggtgcccc agcaactcaa
    34381 ggtttcaagt ctgaaaaatc aactcttcgc gctttagaag aaatcgaatc aggaaacgtt
    34441 acaattcacc cagatccaga aggaaaacgt gaagcagtgc gtcgccgtat cgaagaagag
    34501 aaacgccgca aagaagaaga agaaaagaaa atcaaagagc aaattgctaa agaaaaagaa
    34561 gatggtgaaa aaatttaagg ttggggggac tcaatcttat tttttctatt gcaagaatgt
    34621 actgacaagg aggaggtgag aagatggccc tagctaagat tatcgtagat gtgcccttga
    34681 tgcagacgga ccagccctat agttacagga ttccggagga atttgaggga atgctggaag
    34741 ttgggatgcg ggttcatgta ccttttggta agggtaatcg cctgattcaa gggattgttc
    34801 ttggtttgga gtcccaatca gatggagaag agatggagca agatttaaaa gatattgcag
    34861 aggtgctgga tttttctcct gttctcacgc cagaacaact ctggctggct gaggagttac
    34921 gcaaatctgt cttctcctac aaaatctcta tcctcaaggc catgcttcca ggatttctga
    34981 attctagcta tgacaagatt ctctatcctc tggaaggctt gagtcaggaa gaacgagtac
    35041 gcctgtttgg ttcagaagat tcgctagcct tttcttcact agaccttgcc aagcaagctg
    35101 aaatgatgcg tttgactaga aaaggcctgc ttggtctgga atatcaggca gtcgatcaaa
    35161 agaaggtcaa gacccagtct tggtatgagg ttgaccatac tcaattagaa ggtgttgaga
    35221 tttctacacg tgccaagaaa aagttggaac tgagagatta tctgctgtct catccagaga
    35281 gctcttcctt ggctaacttg ttagagtcct actcgcgaga gcaagtcaac ttctttgtgg
    35341 atcaaggtgc tgttaccata gtccaaaagg aagttcaacg ctcggctgct tattttgaag
    35401 gaattgaagc aagtagacct ttggagttaa atccagaaca aagacaggcg catgatgcgg
    35461 ttgtgagttc tattggcagt tctcaacctc cctttctact tcaagggatt acaggaagtg
    35521 ggaagaccga ggtttacttg cagattatcc aaggtgccct ggataagggc aagacagcta
    35581 ttttgctggt acctgagatt tccttgactc ctcaaatgac cgagcgtttt atagcacgct
    35641 ttggggacaa ggtagccatt cttcactcag gattatccaa tggtgaaaag tatgatgaat
    35701 ggcgcaaggt ggagcgtggc gatgcccaag ttgttgttgg tgccagatct gctatctttg
    35761 ctcctctgaa aaatctgggt gtcatgatta ttgatgaaga gcatgaagcg gcttataagc
    35821 aggacagcaa tccccgttac catgccagag aggtagctat tttacgggct cagtataatc
    35881 aagcaactct ggtacttgga tctgcaacgc cgagcttaga gagccgggcg cgtgctggca
    35941 aaggcgtcta tcaacactta cgtctaaccc aacgtgccaa tcctttggct acaatccctg
    36001 aggttcaagt gattgacttt cgagactata tcggacaaaa tgagacgtca aactttacgc
    36061 ctcctttgct agaggctatc caagaccgtc tggttaaaaa agagcaggtg gttctcatgc
    36121 tcaatcgccg tggttattct agctttgtca tgtgtcggga gtgtgggacg gtggatactt
    36181 gtcccaactg cgatatttct ctgaccttgc atatggatac caagactatg aactgccatt
    36241 attgtggttt ctcgaaggat attcctcagg tctgtcctaa ctgtaagagc cacagtattc
    36301 gttactatgg gacgggaact cagaaggctt atgatgagct agcagaactc tttccccaag
    36361 ctcgcattct gaggatggat gtggatacga ctcgaaagaa aggcagtcac caagccttgc
    36421 ttgaccagtt tgggcgaggg gaagcggata ttttacttgg tactcagatg attgcaaagg
    36481 gtttggattt tcccaatgtt accctagtcg gagttctaaa tgcggatacg gccttgaatc
    36541 tgcctgattt ccgttcttct gagagaacct tccagctctt gactcaggtg gcaggtcgag
    36601 caggtcgggc tgaaaaggca ggtcaagtct tgatacagtc ctacaatccg cagcactatg
    36661 ctattcgatt tgccaaggat caagattacg aaggctttta tgcctatgaa atgggaatca
    36721 gacgacaact cggctatcca ccttactatt tcacgattgg tattaccctt tctcacaaga
    36781 aagaagaaga ggttttcaaa cgtgcctatg aagtcatgaa cattttgcgg tcaggcttgt
    36841 cagagaccag tcccattctg gggccaacgc caaaaccgat tgcccgtacc cacaacctct
    36901 atcattacca gattttaatt aaataccgtt tagaagatga gctggggccg accctcaacc
    36961 aggtcctggc cttgactcaa gaacgggaaa atagtgagct ccgtctcagc attgaccatg
    37021 agccgcagca atttttataa gaaggagaag atatgacaaa actaatcttt atggggaccc
    37081 ccgacttttc agcaacagtc ttaaaaggac ttttgacaga tgaccgttac gaaattctag
    37141 ccgttgtgac ccagccagac cgtgctgttg gtcgtaaaaa agttatccaa gaaaccccag
    37201 tcaagcaggc tgccaaggaa gcaggattat ctatctacca acctgaaaaa ttatctggaa
    37261 gtccagagat ggaagatctt atgaagctag gagcagatgg aattgtgact gctgcttttg
    37321 ggcagtttct cccaagcaaa ctccttgata gcatggactt tgctgtcaac gttcatgcct
    37381 ccctccttcc tagacaccgt ggtggtgcgc ctatccatta tgccttgatt caaggggatg
    37441 aggaagctgg tgtgaccatc atggaaatgg ttaaggaaat ggatgcagga gatatgattt
    37501 ctcgtcgcag cattccgatc acagatgagg acaatgttgg caccttgttt gaaaaattgg
    37561 cgctagttgg tcgtgatttg cttttggaca ctctgcctgc ctatattgct ggtgatatca
    37621 aacctgaact gcaggatacg agtcaggtta ccttctctcc aaatataaag ccagaggaag
    37681 aaaaactgga ctggaacaaa accaatcgtc aactctttaa ccaaattcgt ggaatgaacc
    37741 cctggcctgt tgcccatact ttccttaagg gcgaccgctt taagatttat gaagccctac
    37801 cagtagaagg tcagggaaat ccaggtgaga ttctctctat cggcaagaaa gaattgattg
    37861 tcgcaacggc tgaaggggct ctatccctca aacaagtgca gccagctggt aagcctaaga
    37921 tggacattgc ttccttcctc aacggagttg gacgtacatt gactgtagga gaacgatttg
    37981 gtgactaaag tagaaacggc tagaagttta gctctagcag tgctagagga tgtttttgtg
    38041 aatcaagcat attcaaatat tgccttaaat aaacacctca aggggagtca gctctctgca
    38101 gcagacaagg gcttagtgac cgagctggtc tatggaacgg tagcccgtaa actgactctg
    38161 gaatggtacc tatcccactt tatcgaagac agagaccagt tagacaactg gctctatgtc
    38221 cttcttctca tgagtgccta ccaactccgc tatttggaca agattccaga tcatgctgtg
    38281 gtcaatgaag cagtggaatt ggccaaactc cgtaaaaaag gcagtgaaaa attggtcaac
    38341 gctgtccttc gccgtatctt gcgtgaaggc tggccagata ttgctagcat caagcgaaaa
    38401 aacaagcgtg actccattgc ctattctctc ccagtttggc tagttgccaa actcaaggaa
    38461 gaatacggag aggagagagc aaaagccatc tttgaaagcc tgttagtgcg aaacaaagcc
    38521 agtattcgtg taacagacct aagtcgaaaa gaggaaatcc aagccttgtt ggaggcgaat
    38581 aattcccttt tagcaaccac tggtctggtt aaggagcaag ggcattttgc agggcataat
    38641 ttgtttacga atggagccat taccatccaa gacgagtcca gtcagctggt tgctccgacg
    38701 cttgatttac aaggtgatga gcgagtgctt gatgcctgtg cggctccagg tgggaaaaca
    38761 gcccatatag cctcttatct tacgacaggt caggttactg ctctggactt gtacgaccac
    38821 aagttggatt taatccaaga aaatgcccaa cgtctgggag ttgcagatcg ggttcaaact
    38881 caaaaattag atgccagaaa ggtgcatgag ttttttgacc aggattcctt tgataagatt
    38941 ttggtggatg ctccttgttc aggaatcggt cttttgcgcc gaaaaccaga tatcaaatac
    39001 aataaagaaa cggcagattt cgcgtccttg caggaaattc agttagaaat attaggtagt
    39061 gtttgtcaaa tactaggcaa aggtggtata ataacttata gcacctgcac tatcgtctca
    39121 gaggagaatt ttcaagtcgt taaggccttt ttagagagtc atcccgagtt cgagcaggta
    39181 aaactagaac atgaatgtaa ggatatcatg aaagacggct gtatcctcat tacacctgaa
    39241 ttgtatggaa gtgatggatt cttcatcagt caatttcgca agatatcgga ttaggaagga
    39301 actgacacat ggaaatttca ttattaacag atgttggtca gaaacgaaca aataaccaag
    39361 actatgtcaa ccactatgtc aatagagctg gacgtaccat gattatttta gctgatggga
    39421 tgggaggtca tcgcgcaggg aatatcgcta gtgaaatggc ggtgacagac ctgggtgtag
    39481 cttgggttga tacccagatc gatacagtca atgaagtgcg tgaatggttc gcccattacc
    39541 tagaaattga aaatcaaaag attcaccagc ttggtcagga tgaagcttac agaggcatgg
    39601 gaactacttt ggaagtcctt gctattattg ataatcaggc tatctatgct catattggtg
    39661 attcgcgtat cggcttgatt cgtggagaag aataccatca gttgacgagc gatcattctt
    39721 tggttaatga attgctcaag gctggtcaat tgacaccaga agaggcagaa gctcatccac
    39781 aaaaaaatat tatcacccag tctattgggc aaaaagatga aattcagcct gattttggga
    39841 cagttatcct tgagtcaggt gactatctct tgctcaatag tgacggcttg accaacatga
    39901 tttcaggcag tgagattcgt gatattgtaa ccagtgatat tcctttagca gataaaacgg
    39961 agacacttgt tcgttttgct aacaatgcag gaggtttaga caacattacg gttgcccttg
    40021 tttctatgaa cgaggaggat gcagaatgat ccaaatcggc aagatttttg ccggacgcta
    40081 tcggattgtc aaacagattg gtcgaggagg tatggcggat gtctacctag ccaaagactt
    40141 aatcttagat ggggaagaag tggcagtgaa ggttctgagg accaactacc agacggaccc
    40201 gatagctgta gctcgttttc agcgtgaagc gagagctatg gcagatctag accatcctca
    40261 tatcgttcgg ataacagata ttggtgagga agacggtcaa cagtatcttg caatggagta
    40321 tgttgctgga ctagacctca aacgctatat caaggaacat tatcctcttt ctaatgaaga
    40381 agcagtccgt atcatgggac aaatcctcct agccatgcgt ttggcccata ccagaggaat
    40441 tgttcacagg gacttgaaac ctcaaaatat ccttttgaca ccagatggga ctgccaaggt
    40501 cacagacttt gggattgctg tagcctttgc agagacaagt ctgacccaga ctaactcgat
    40561 gttgggctca gttcattact tgtccccaga gcaggcgcgt ggttcgaaag cgactgtgca
    40621 gagtgatatc tatgctatgg ggattatttt ctatgagatg ttgacaggcc atatccctta
    40681 tgacggggat agcgcggtga ccattgccct ccagcatttc cagaaacccc tgccgtccgt
    40741 tattgcagaa aatccatctg tacctcaggc tttagaaaat gttattatca aggcaactgc
    40801 taaaaagttg accaatcgct accgctcggt ttcagagatg tatgtggact tgtctagtag
    40861 cttgtcctac aatcgtagaa atgaaagtaa gttaatcttt gatgaaacga gcaaggcaga
    40921 taccaagacc ttgccgaagg tttctcagag taccttgaca tctattccta aggttcaagc
    40981 gcagacagaa cacaaatcaa tcaaaaaccc aagccaggct gtgacagagg aaacttacca
    41041 accacaagca ccgaaaaaac atagatttaa gatgcgttac ctgattttgt tggccagcct
    41101 tgtattggtg gcagcttctc ttatttggat actatccaga actcctgcaa ccattgccat
    41161 tccagatgtg gcaggtcaga cagttgcaga ggccaaggca acgctcaaaa aagccaattt
    41221 tgagattggt gaggagaaga cagaggctag tgaaaaggtg gaagaagggc ggattatccg
    41281 tacagatcct ggcgctggaa ctggtcgaaa agaaggaacg aaaatcaatt tggttgtctc
    41341 atcaggcaaa caatccttcc aaattagtaa ttatgtcggc cggaaatcct ctgatgtcat
    41401 tgcggaatta aaagagaaaa aagttccaga taatttgatt aaaattgagg aagaagagtc
    41461 caatgagagt gaggctggaa cggtcctgaa gcaaagtcta ccagaaggta cgacctatga
    41521 cttgagtaag gcaactcaaa ttgttttgac agtagctaaa aaagctacga cgattcaatt
    41581 agggaactat attggacgga actctacaga agtaatctca gaactcaagc agaagaaggt
    41641 tcctgagaat ttgattaaga tagaggaaga agagtccagc gaaagcgaac caggaacgat
    41701 tatgaaacaa agtccaggtg ccggaacgac ttatgatgtg agtaaaccta ctcaaattgt
    41761 cttgacagta gctaaaaaag ttacaagtgt tgccatgccg agttacattg gttccagctt
    41821 ggagtttact aagaacaatt tgattcaaat tgttgggatt aaggaagcta atatagaagt
    41881 tgtagaagtg acgacagcgc ctgcaggtag tgcagaaggc atggttgttg aacaaagtcc
    41941 tagagcaggt gaaaaggtag acctcaataa gactagagtc aagatttcaa tctacaaacc
    42001 taaaacaact tcagctactc cttaaaagca gatggataat caaaatgagt aatcaatctt
    42061 tgtcatgatt tcatggcaaa gatttttttt gagtccggat ttgtgataga atagagggag
    42121 ttgataaaag gaggaaagca tggaagaatc aaaagaatta aatgccgtca ttgatgtgat
    42181 tatgctagcg gggactattc tccttaaaag tggctcagaa atccatcgtg tagaagatac
    42241 catgattcga atcgcgcatt cgcaggggat tgtggattgc aatgtccttg ccatgcctgc
    42301 cgctatcttt ttctctattg aaaataccaa tatttcgcgc atgaagcgcg tgacctcctc
    42361 ttcttataac atcgaaaaag tctgcgatgt gaaccagatt tctcgtcagc tggttggggg
    42421 gcagattgat ttagaaacag cctttaagca attgacggcc ttgcaagctc aaccccttcc
    42481 ctatactaag ttgcaggtaa ctctggctgc gacctttagc gctcctttct tttcagttat
    42541 gtttagcgga aatatctacg acgcacttgg ggcaggagtg gcgaccttat ttggttttgc
    42601 cttttccctc tatgtggaaa aatttatccg aattcccttt gtgacagcct ttgctggagc
    42661 ctttgtcttt gggataattg cccagttttg ggctcgctac acaggttttc cttcaacggc
    42721 agatttgatt atagctggtg cggtcatgcc gtttgtacca ggtattgcct tgaccaatgc
    42781 ggtccgtgat attatgacca accacataaa ctctggtatg agtaagatgt ttgaatccct
    42841 gctcattacc ctcgctttag gggcaggaac ttctgtcgcc ttggtattga tgaactaata
    42901 tgacactaac aaccttttta ttacaagcag tagcaagttt tcttgccatt atcacttttt
    42961 taatcgtact caatgtgcaa cggtctatgc tcttacctgg agggattttg ggcatgactg
    43021 tctggctaat ctatctcttg ctcaaggaac cgaccaatgt cattgtagct accttcattg
    43081 cagccattat tggttcttgt gtcagccaga ttttaagtat tctttataag acacctgctg
    43141 tggtctttat cttggccatt ttggcaccgc tggttccagg ttatctctcc taccgaacaa
    43201 ctgccttttt tgtgacaggg gactataata aagcactggc aagtgcgacc ttggttgtca
    43261 tgttggcttt ggtaatctct attggaatgg ctagcggaac agtgattctc agactgtatc
    43321 attatataaa aacacatcga gtatcgtaga ctttacagaa ataaaagaat tttctgaaaa
    43381 atgagataaa taaattaaca acgctttcta tatgtgcgag aataccgcac ttatgaacaa
    43441 attgcggctg attttggtat ccacgaaagc aacttactcc gtcggagcca atgggttgaa
    43501 gtaactcttg ttcaaaggtg gtgttgcgat ttcaagaact cctctcagtt ctgaagacac
    43561 ggtaatgatt gatgcgacgg aagtaaaaat caatcgccct aaaaaaaaca attagcgaat
    43621 gattctggta aaaagaaatt tcacgctatg aaggctcaag cgattgtcac aagtcaagga
    43681 agaattattt ctttggatat cactgtgaac tattgtcatg atatgaagtt gttcaaaatg
    43741 agtcgcagaa atatcggaca agctggtaaa atcttggctg acagtggtta tcaagggctc
    43801 atgaagatat atcctcaagc acaaactcca cgtaaatcca gcaaactcaa gccgctaaca
    43861 gttgaagata aagcctgtaa ccatgcgcta tctaaggaga gaagcaaggt tgaaaacatc
    43921 tttgccaaag taaaaacgtt taaaatgttt tcaacaacct atcgaaatca tcgtaaacgc
    43981 ttcggattac gaatgaattt gattgctggt attatcaatt atgaactagg attctagttt
    44041 tgcaggaagt ctactacttt ccttattgtc tgtaaatcta ctgaccttgt tgtttatccc
    44101 agtcatggtt tctagttcgg gctcagagtt tcaaagtgga tggcaagagc atcaattgat
    44161 tgctgagaag gttagtaaaa cacttgacaa gacatttgat aaggatgtca gagaaattcc
    44221 gaccagtcag ttttatcaaa aatttgtaga tgagatggga aggatttact caggaaattt
    44281 gatcctccag gagctgataa ctgtgaatgg agcttataaa gctacttata tcggtgaact
    44341 ctctagcaac taatcattct ttaaaatgat gaccaataag aggcttgatt tggtgaatca
    44401 agtctttttt ctctctttta ctgccatttg tgataaaata gtatagaacg attttttaca
    44461 tgaatgataa aacagaggta aatatgacaa tcggtattga taagattggt tttgcgacca
    44521 gtcaatatgt cttgaaatta caagacttag cagaagcgag gggaattgac cctgaaaaat
    44581 taagtaaagg actcttactc aaggaattga gtattgcgcc cctaactgag gacatcgtga
    44641 ccttggcggc cagtgctagt gactctattt taactgagca agaaagacaa gaagttgaca
    44701 tggtcattgt ggctaccgag tcaggaattg accagagtaa ggctgcggcc gtctttgtgc
    44761 atggcttgct gggcatccag ccctttgctc gtagtttcga gattaaagaa gcctgctatg
    44821 gggcgacagc tgccctccat tatgccaaat tgcatgtgga aaattctccg gagtccaagg
    44881 tcttggtcat tgccagtgat attgccaaat atggtattga aactccggga gaaccaactc
    44941 aaggtgctgg aagtgtagct atgttgatta cacaaaatcc acgcatgatg gcctttaata
    45001 atgacaatgt agctcagacc cgtgacatca tggatttctg gcgaccaaat tactcgacaa
    45061 ctccttatgt aaatggtgtc tattctaccc aacaatactt ggatagtttg aaaacgactt
    45121 ggcttgaata tcaaaaacgc taccagctta ctttggatga ttttgcggct gtttgtttcc
    45181 acttgcctta tcctaaatta gcgctaaaag gcttgaaaaa aatcatggat aagagcctgc
    45241 ctcaagagaa aaaagacctc ttacaaaagc attttgacca gtctattctc tacagtcaaa
    45301 aggtggggaa tatctacaca ggttcacttt tccttggact tttgtctctc ttggaaaata
    45361 cagatagctt gaaagctggg gataaaatcg tcctttatag ttacggaagt ggagctgtgg
    45421 ctgagttctt cagtggtgaa ttggttgaag gatatgaagc ttatttggat aaagaccgct
    45481 tgaacaagct caaccaacga actgccctat ccgttgcaga ctatgaaaag gtcttttttg
    45541 aggaagtaaa cttggatgaa acaaactctg cccagtttgc tggctatgaa aatcaagatt
    45601 ttgccttggt tgaaattctt gaccaccaac gccgttatag caaggttgaa aaataatgaa
    45661 gataagttgg aatggatttt ctaaaaaatc ataccaagag cgcctcgagc tgctaaaagc
    45721 tcaggcgctc cttagtcctg agagacaagc tagtctggag aaggatgaac agatgagtgt
    45781 gactgtggca gaccagctga gtgagaatgt ggtgggaact ttttctctgc cttattcgct
    45841 ggttccggag gtacttgtca acggtcagga atacaccgtt ccctatgtga cagaagaacc
    45901 ctctgtggtt gcggcggcca gctatgccag caaaatcatc aagcgtgcag gtggttttac
    45961 tgcacaagtc catcagcgcc agatgattgg gcaggtagcc ctttatcaag ttgctaatcc
    46021 taaactagcg caagagaaga ttgccagcaa gaaagcggag ctcttggagc ttgccaatca
    46081 agcctatcct tctatcgtta aacgtggggg tggggcgcgt gatctgcatg tcgagcagat
    46141 aaaaggcgaa ccagactttc tcgttgttta tattcatgtc gatacccagg aagccatggg
    46201 tgccaatatg ctcaacacca tgctggaagc cttgaaacca gtcttagaag aactcagtca
    46261 gggacagagt ctcatgggaa tcctgtccaa ctacgcgact gattctctgg tgactgcaag
    46321 ctgtcgcatc gcctttcgct acttgagccg ccaaaaggat caaggacgag agattgcgga
    46381 gaaaattgcg ttggctagtc agtttgcgca ggctgatcct taccgagctg ctactcataa
    46441 taaaggaatt tttaatggta ttgatgcgat tttgattgcc actggtaatg actggcgtgc
    46501 catcgaagct ggggcccatg cctttgccag tcgagatgga cgctatcaag gtcttagctg
    46561 ctggacgctg gaccttgaaa gagaagaatt ggtcggtgag atgaccctgc ccatgcctgt
    46621 agcgactaag ggtggctcta tcggcctcaa cccacgtgta gctctcagtc atgatctact
    46681 aggaaatcct tctgccagag aattagccca gattatcgtg tccatcggtc ttgctcaaaa
    46741 ttttgcagcc ctcaaagcct tggtaagtac gggcatccag caaggccaca tgaaactaca
    46801 ggccaaatcc ctagctctcc tagctggggc tagtgaatct gaagttgctc ccctagtaga
    46861 gcgcctcatc tcagataaaa cctttaacct agagacagcc cagcgctatc tcgaaaattt
    46921 aagatcataa aaactcagac gaatcggtct gagttttctt gtgtttaaat actttttcct
    46981 ggtaatagcg taactggtaa gaagtaacca gttgtggcga cttccttgcc ttcaatcttt
    47041 tgcaagagaa gatcaatagt gagacaagca atctcttcca aaggttgctt gatagtagcc
    47101 aattgagggt agtaattttc gataaagtag gtcccatcat agccgatgac cttgagctct
    47161 tttgggacag aaatgcccaa ttcttgagcg attttaatga ccagaatagc tgtcaaatca
    47221 tccgaagcaa aaatggcatc tggtttttcc cgggtcaaga tattcttgat ttccatttct
    47281 tttctgacgg gagaaaagtc actggaaaca ttgataatag gagcttttgg gagtacggat
    47341 gcaaaaccag cgtggcgcag tccggttggc gaattggaat tgtcattccc tgtaatcatg
    47401 atgatagact gggcacctgt cttgaccaag gtttgggcag caagaacccc accagcatag
    47461 ttgtcagagg agacaacagg gatgtctggc gataggtttc ggtcaaagga aataatcggc
    47521 gctgtcacac gattgtagtc ttcgattcct aggttgtgac taccagaaat gatgccgtct
    47581 acctgattgg cttccaacat ttcgatgtat tcgcgttcct tctcagaatc atgttcactg
    47641 ttgcagatga tggtcttgta accatttttg aagagttggt gttccaattt atcaatcaat
    47701 tctgcataga aaacattgga aatattgggg aaaatcaagc cgattaactt agctgatttt
    47761 ccttgcagac tacgagccag gttgttgggt ttatagccta attctctcat ggcttcattg
    47821 actttttgga tggttttctc agatagatat ccttttttat tgataacccg agaaacggta
    47881 gtaggactga cgcctgcaag tttggcgaca tcagttagtt ttgcgaccat aatctaattc
    47941 atagtaagtt ccagttgggt ttccagattt gatcaggata ccattttggt ccgcatgtgg
    48001 gaagacacga ccagaaaata ctttttctcc tttattgatg aaaatttcaa agacagagtt
    48061 atcgatgaag attgtagcag tagtagcctg attctcgata gggcaagagc gagttgtccc
    48121 aaattcttgg gcatactgtt ctccagcctg gctacgatcc actgtcactt gaccgtttac
    48181 aaggtcaaag ttgattgaaa gtcccttacc ttctttatca gcaagtaaga caatctcgct
    48241 ctggctatta gcttccaagt tgagttcaag ttcgtaagtg ttcttggttt gggaacggtt
    48301 tgagaaggct tcttcagaag cacgaaggtc cttaatagca gcgactggat actggtagag
    48361 cttgtcgtct ttgatagtga gttccttgac caaagagaag gttccttggt ggtcaaaacg
    48421 gtcagatggg taagaaacat ctggcaaacc aagccagcta actgctagag cacgcccatc
    48481 aggagcgttg aaggcttgag ttgcataggc ttcgaaaccg taatccatgt tttgaagttg
    48541 agacacatct accattttgg catttttagg gtcaaaggaa gccccaatct tatacatatt
    48601 tggaaagata ttatcgtagt ctagaacttt cttatccaat ccttgtggac agtagagaag
    48661 gacaggctgt tcctctacaa agaccagatt aggacattcc atcatgtagg cagtacggtc
    48721 gttagcaaag tcaaggtcgc caactgcttg ccagtttgtg tagtcgttat tgacagcctt
    48781 gtagagacgg acgaagcctt ttttctccaa gtcctgtcca ccgacgacag catagtattg
    48841 acctttaaag ttaaaaattt gtggatcgcg gaagtggtca gtagagtctg ctggctggtc
    48901 aatcaagatc ttgtcaatct ttgtaatctt accctccttg tccatcaaag caccgatttg
    48961 gtatgggtga cggatccagt tttcatcgcg aacatttcct gtataaaata ggaataagtt
    49021 atcgccaaat tgcatggcag aaccagagta ggcaccgtgg ctatctaatg gagtatctgg
    49081 taaaactttg attccagttt ctttaaagtg aaccaaatca tcactttcta gctgtgccca
    49141 agatttcaaa ccgtgggctg caccaaaagg aaaattctgg taaaagagga tccacttgcc
    49201 atcaaagtaa gaaaagccat ttgggtcgtt gagaagtcct gtttttggct caacatggta
    49261 atgagtatgc catggagatt gtgccatatt ttcctttata tgttgaattt cttcttgcgt
    49321 ccaatcttga taaagtctgt aacgacgctc agttgtccat tccattcatt cctccaaaaa
    49381 tcttatcact tttaatagta actgttttcg ataaaaaatg caagcctttt atgttaaaaa
    49441 agaggaaaaa ctgtcaaacg attatcataa aataaataca ggaaatcaat caaattttta
    49501 cgaaaatgtg aaagataatg aaataaaaga ctttttaacc aaggtttcaa acttattttt
    49561 caaggaattc ctgatgaaac agtcaaaaac aatcaatcta aaacttgccc actaagtgga
    49621 tagaatattt tttctgcaaa acgcttgaca tatttttaaa acatgataaa ataatagtcg
    49681 taagggcgaa taaaatcgct ttcaaacaag aacaaaatgt tatataagga gatttttgca
    49741 aatgaacaat caggaaattg caaaaaaagt catcgatgcc ttgggcggac gtgaaaatgt
    49801 caatagtgtt gcccactgtg cgactcgtct acgtgtcatg gtcaaagatg aagagaaaat
    49861 caataaagaa gtgattgaga acttggaaaa agttcaaggt gctttcttta actcagggca
    49921 ataccaaatt atctttggta caggtacagt taacaaaatg tacgatgaag ttgttgtact
    49981 tggcttacca acatcatcta aggatgacat gaaagcagaa gttgctaaac aagggaactg
    50041 gttccaacgt gctatccgta ctttcggtga tgttttcgtt ccaatcatcc cagttatcgt
    50101 agcgacaggt ctcttcatgg gtgtgcgtgg tcttttcaac gctcttgaaa tgccacttcc
    50161 aggtgacttt gcaacttaca cacaaatctt gacagataca gccttcatca tcttgccagg
    50221 tttggttgtg tggtcaacct tccgtgtatt tggtggaaat cctgccgttg gtatcgttct
    50281 tggtatgatg cttgtctctg gctcacttcc aaacgcttgg gcagttgctc aaggtggtga
    50341 agtaacagcg atgaacttct ttggtttcat ccctgttgtt ggtttgcaag gttccgttct
    50401 tccagccttc atcatcgggg ttgtcggagc taaatttgaa aaagctgtcc gcaaggttgt
    50461 tccagatgtc attgacctct tggtaacacc attcgtgaca cttttggtca tgtctatcct
    50521 tggactcttt gtcattggac cagttttcca cgttgttgaa aactacatcc ttattgctac
    50581 aaaagcgatt cttagcatgc catttggtct tggtggtttc ttgattggtg gggttcacca
    50641 attgatcgtc gtgtcaggtg tgcaccacat cttcaacttg cttgaagtgc aattacttgc
    50701 tgctgaccat gctaacccat tcaacgctat catcacagct gctatgacag ctcaaggtgc
    50761 tgctactgtt gcggttggtg ttaaaacaaa aaatccaaaa ctgaaaacac ttgctttccc
    50821 ggctgctctt tctgccttcc taggtattac agagcctgct atcttcgggg tgaacttgcg
    50881 cttccgtaaa ccattcttcc tttcattgat tgctggtgca atcggtggtg gattggcttc
    50941 tatccttgga cttgctggta ctggtaatgg tatcaccatc atccctggta caatgcttta
    51001 tgttggtaac ggacaacttc cacaatacct tcttatggta gctgtatcat ttgcccttgg
    51061 ttttgctctt acttacatgt ttggttacga agatgaagta gacgcaactg cagctgcaaa
    51121 acaagctgaa gtggctgaag aaaaagaaga agttgcgcca gcagctcttc aaaatgaaac
    51181 acttgtaact cctatcgtcg gtgatgttgt cgctcttgct gatgtcaatg acccagtctt
    51241 ctcaagtgga gctatgggac aaggtatcgc tgtgaaacca agtcaaggcg tggtctatgc
    51301 actagctgat gctgaagttt caattgcctt tccaacaggg cacgcttttg gtttgaaaac
    51361 aagaaatggt gctgaagttt tgattcacgt tggtattgac actgtatcta tgaacggtga
    51421 cggttttgaa gcaaaagttg ctcaaggtaa taaggtgaaa gctggcgatg ttcttggaac
    51481 atttgactca aacaaaatcg ctgcagctgg acttgatgat acaacaatgg ttatcgttac
    51541 aaatacagct gactacgctt cagtagctcc agtcgcaaca ggttcagttt cgaaggggga
    51601 tgctgtgatc gaagtgaaaa tctaatcaat cctctctaat gtgaaaacga acaaatgaca
    51661 tgtttgttcg tttttattag atagtaagat ttacagagga aaatctaaaa aatagagaca
    51721 tttagacttt cgaagtatgc tataataaag aaaataaaaa caagaggttt atcatgacaa
    51781 aattatatgg aagcttggaa gcgggcggta caaagtttgt ctgtgctgtc ggtgatgaaa
    51841 actttaacgt tgtagaaaaa acacaatttc caacaacaac tccaatcgaa acaatcgata
    51901 aaaccattga gttcttctca aaattcgata accttgctgg tcttgcagtt ggttcatttg
    51961 ggccgattga tattgacaaa aactcaaaaa cttatggctt tatcacgacg actccaaaac
    52021 caaactgggc aaatgtagac ttgcttggtg cccttcgtcg cgccctaaac gtgccaatgt
    52081 acttcacaac agacgtaaac agctctgctt acggtgaaat ggttgcccgt aacaatgctg
    52141 gtggtcgtat cgaaaacttg gtttactaca caatcggtac aggtatcggt gcaggcgtca
    52201 tccaacgtgg tgagtttatc ggtggtgtgg gtcaccctga aatgggtcat tattatgttg
    52261 ctagacaccc aatggatatt gaaaaagagt ttaagggtgt ttgtcctttc cataagggat
    52321 gtctagaagg ttatgcagct ggtccaagtt tggaagctcg tacaggtgta cgtggggaaa
    52381 atattgaact caacaaccct gtttgggatg ttcaagccta ctatatcgct caagctgcgg
    52441 ttaatgcgac agtgactttc cgcccagacg tgattgtctt tggtggaggg gtcatggctc
    52501 aacaacatat gctggaccgt gtccgtgaga aatttacatc tcttcttaat ggttacctac
    52561 cagtaccaga tgtgcgtgac tatatcgtga cgccagcagt cgcaggaaat ggttctgcca
    52621 cacttgggaa ctttgttctt gcaaaagaag tttcaaaata aagcacaaaa tccgcttggg
    52681 aaaccaaacg gattttgtgg ttgaaaacaa tatttttgag cctttcctta agtgattacg
    52741 gacggtagcg actttcttcg aaattccata cctaaacttt gagcctagtg tctcaaagtt
    52801 tccgaatacc tgaaaccatt agatttcagg tgtttctatc acggcggaaa ggctaggtaa
    52861 gtcctcgcct cactatcgaa aaatatgcat aatttccata ccaaagtata tttagtatca
    52921 agctatagtt aatctacgct ttaaaaatcc acttgggaaa ccaaacggat tttttacgtg
    52981 tcaaagcatt tgccagaaaa agtcaataat ataggtcaga ccgtaggtat aaaccacgag
    53041 ggtaaagggt ttggtcagaa tgatgatagt catgtagcgc ttgaaactca tcttggtcag
    53101 ggcggccagc atacagagaa agtcagctgg gctaatgggc caaatcatca taaaaataaa
    53161 gaagcggtca aaacgattgc ccttatctag ccagccgatg tacttgtcgt aggtgcgctt
    53221 gctgacgaca gactggacaa aggcagctcc gtataggcgc accagataaa agataatggc
    53281 acagccaatc acgatgccga tatagttgta gatagtcccg atgatgtgcc cgtagataaa
    53341 gaccccagcc accgaggtca aggcccctgg aatgataggg acgacagtct gtaaaatctg
    53401 taaaaagata aagagaggtg gaccccagat gcctgcctgc tggataaagg cagagagggt
    53461 ttccttggat tgtaaaatcc cagcctgata agcccaaata cagaaaatca aactcccaac
    53521 cccaccgaca atcgatgaga tattgataac tcgctgcaga aggggagaag cagctttcat
    53581 ttgtttatcc tgtgacatgt aaactcctca tagatagtat atttctacta taccatattt
    53641 tagaggagaa cgggggaatg agtctttttt gataaggaat agaaagtaaa tagcccttcg
    53701 agacttagaa taggtctgtc aactaaaggt tatatgcatt ctaggtttcg aataatcgca
    53761 attccataaa tttaaaagca ttctaagttt cgaatagctt atctgagata attttgaggc
    53821 attcgcgctt tcgaatagtc attttcccta aagtttagat gtattctgaa gctagaaaga
    53881 ctaaaatata aaagtagtaa gagtctctct ctgatggacg taatttatgg ctagtatgaa
    53941 tcctttgaat tgttagttaa ttttaatatt aatcagttct gaaataatta ctaaatatag
    54001 ttagatggtg aattaattat tgctaaattg caagttcaag ttagtcatcc agaagtcttc
    54061 tctgggggac ttgtagttca agcatttttt aggatagttg ttaatccact tttcgataaa
    54121 tgcgacttcc tttgtagtca ttttcttagt tccctgaggt aaccatctat gaatgagtct
    54181 gttgtgattt tcattagatc ctatagtgat gtgcataata gatatagcag attgaaacta
    54241 gaatagtata catctacttc taaaacattg ttagaaatca atttgactgt cctgatcaat
    54301 ttgtcctgtt tttatttcat tttactatat aatccttaga aaacatatcg gacaagcggt
    54361 tgaattccgt tccgtccatt agtagataaa ggattactcc ttcttgaaat gacagatgaa
    54421 cttgtttctt ataaaaatgt tgtctgaaac tattcgcttt tttccagaag ttttagagga
    54481 agaaaacttt atgaggaaaa aggagttact caaataacaa ttcaggatta aaaatagaca
    54541 gttgaggagc ggaaggtata aattaagttt gctactgtat aatggattta tcactaatac
    54601 tcttcgaaaa tctcttcaaa ccacgtcagc ttcgccttgc cgtggatata gttactgact
    54661 tcgtcagttc tatccacaac ctcaaaacag tgttttgagc tgacttcgtc agttttatct
    54721 gcaacctcaa agctgtactt tgagcagcct acggctagct tcctagtttg ctctttgctt
    54781 ttcattgagt ataaaagtgc tccaggccat attttacaca gcaatagtct tctaaaaatg
    54841 gacacaaaac gttgtaaagg ctatcaaaaa ggagtataat gagtgcaaga aatttcggag
    54901 gtgaaagatg ctagaagtaa gaagtctaga gaaaagtttt ggctccaagc aagttttgtt
    54961 tggtattgac tttcaagcgc gaccaggtcg tattttggga ctagtcggaa aaaatggtgc
    55021 tggaaaaaca acgattttcc atagtatttt gaaattccta gaatatcagg gagaaatcgg
    55081 tctggatggt caggatattc gtcaggagac ctatgctcgg attggttatc tgcctgaaga
    55141 acgcagtctc atgcctaaat tgacagttct tgaacaagtt cgctacttgg cgactctaaa
    55201 aggcatggat gccaaagaag tcaaagaaaa actccctcaa tggatgaaga ggttggaagt
    55261 gaaaggaaag ctgacagata aaatcaagag tctgtcaaaa ggaaatcagc agaagattca
    55321 gctcattatt actctgattc atgaaccaga cctgattatc ttggatgagc cttttagtgg
    55381 attggaccca gttaatacag aattgctcaa acaagtcatt tttcaggaaa aagagcgcgg
    55441 agcaaccatt atcttttctg accatgtcat gaccaatgtt gaggaacttt gtgacgatat
    55501 tctgatgatc cgagatggcc gtgtggtctt gcacggacca gttcaggatg tccgcaatca
    55561 atacgggaaa acgcgtctct ttgtttcaag tgaacgaagc aaggaagaat tggaaaatct
    55621 tcctcatgtc aaacaggtga gcttgaccaa acaaggcagt tggaaattga ttttggagga
    55681 tgagagcgct ggaagggaac tcttcccaat cttgactcaa ggtcaatata tcgcaacatt
    55741 tgaccaacaa gcgccaacta tcgatgaaat ctttaaacta gaatcaggag tggaagtatg
    55801 agaaatatgt gggttgtaat caaggaaacc tatcttcgac atgtcaagtc atggagtttc
    55861 ttctttatgg tgatttcgcc gttcctcttt ttaggaatct ctgtaggaat tgggcatatc
    55921 caaggttctt ctatggctaa aaataataaa gtggcagtag tgacaacagt gccatctgta
    55981 gcagaaggac tgaagaatgt aaatggtgtt aacttcgact ataaagacga agcaagtgcc
    56041 aaagaagcaa ttaaagaaga aaaattaaaa ggttatttga ccattgatca agaagatagt
    56101 gttctaaagg cagtttatca tggcgaaata tcgcttgaaa atggaataaa atttgaggtt
    56161 acaggttcac tcaatgaact gcaaaatcag cttaatcgtt caactgcttc cttgtctcaa
    56221 gagcaggaaa aacgcttagc gcagacagtt caattcacag aaaagattga tgaagccaag
    56281 gaaaataaaa agtttattca aacaattgca gcaggtgcct taggattctt tctttatatg
    56341 attctgatta cctatgcggg tgtaacagct caggaagttg ccagtgaaaa aggcaccaaa
    56401 attatggaag tcgttttttc tagcataagg gcaagtcact atttctatgc gcggatgatg
    56461 gctctgtttc tagtgatttt aacgcatatt gggatctatg ttgtaggtgg tctggctgcc
    56521 gttttgctct ttaaagattt gccattcttg gctcagtctg gtattttgga tcacttggga
    56581 gatgctatct cactgaatac cttgctcttt attttgatca gtcttttcat gtacgtagtc
    56641 ttggcagcct tcctaggatc tatggtttct cgtcctgagg actcagggaa agccttgtcg
    56701 cctttgatga ttttgattat gggtggtttt tttggagtga cagctctagg tgcagctggt
    56761 gacaatctcc tcttgaagat tggttcttat attcccttta tttcgacctt ctttatgccg
    56821 tttcgaacga ttaatggcta tgcgggggga gcagaagcat ggatttcact tgctattaca
    56881 gtgatttttg cggtggtagc aacaggattt atcggacgca tgtatgctag tctcgttctt
    56941 caaacggatg atttagggat ttggaaaacc tttaaacgtg ccttatctta taaatagaag
    57001 agccttgcga aagcgaggtt tttaaaggtg aaaagggtgg aatcacgaac ctaattatgt
    57061 acacattatg taaacaggtt gcttgaaaac tcaattgtat agtgaactga atctggaata
    57121 ggataatatt acttctaaaa cattcttata aattgatttg aattccaaaa tcccatcgtt
    57181 ccgttcttat ttcattttgc gatatagtga aaggaaataa gtcagctcac aaaaaacgca
    57241 taatatcaag gatttttaac acttgatact atgcgttttc taattttaaa aactttttgc
    57301 ctggtctctt ttgttctatg agtatgttac atatctaggt aaaaattatt taaagcaaat
    57361 taaccttatt tctcaagatg tagtaggttc cgagataaaa gatagctatg aatatgagtt
    57421 ctttaacaat ggctatacct gctcccatat agaaatggtc attgagtcct accagtgaat
    57481 tgacatagaa attagaacta agattgaaga aaagttcaat aaatccaatg acgatttgga
    57541 taccaatgta ggctgcaaca gcgagtgctg tacggtattc attgaaaagc tgtccaatgg
    57601 aaatagccag gtagatgcag aggattcctg aaatggtatt tagtaggaag gatatacctg
    57661 taagaaagat ctgagggaga tgtgtttcta caaatgtaat cacataagaa agaggaatcc
    57721 attctggagc tgttaaagcc agaataataa cagcacttag agccaataca gcggtgctaa
    57781 tcaatgacca gataaaggca ccgattagtt tggctgtgat gatatggtgt tcagaaactg
    57841 gcaaggtcaa agtcagatag ccttgtcggt cgtagacact acctttgaag cgtttaataa
    57901 tcaagaaaat ggttgaaatc gcaagtgtaa gcatcaagcc accaaagact gtagctagaa
    57961 aaagtagcat agtagcctga ctttcttggt aaggtagatt tttaaagcct tgtgtctgta
    58021 ttccgatgag ggcagaaagg actagcacgg ctgcgtagag ggctaaatac cacttgttaa
    58081 catttttaaa ttcgtagtga actaaattcc aaaacataaa taatctcctt tgcttaggcc
    58141 ttaaattcct gacggaagag ttggtcaatg gattcacctg actcgtagcg aatatcatct
    58201 acatttcctt gacggacgac ttttccgtct tttaggaaga caatttcatc caagattggc
    58261 tcgatatcag aaatcaagtg ggtagaaatc aaaacggtag aagttggtga gtagttgttg
    58321 ataatggtat tgaggatata agcacgggct gctggatcca ccccaccaat gggttcatcc
    58381 aaaacataga gacgagcatc acggctcata accaaaatca gttgaacctt ttctttgttt
    58441 ccttttgata gtttcttgag acgactattt tcatcaatgc ccaggtctgc aagtagatga
    58501 tgggcgcgtt caagattgaa atctttatag aaggtcttga agtaggttag ggcttctttg
    58561 accttcattt gctcattgag ataggtcgta tcaggcaaat aagctacaac ggccttggtt
    58621 gctgggcttg ggtccatgtc gttgatgagg acacgtcctt gatctggttg taagaggcca
    58681 ttaattagtt taatcagggt tgtttttcct gagccgtttg gcccaagaag gccgacaatt
    58741 tttccagctg gaatgtcaag agaaacattt tcaagggctg gtgttgctcc atatgatttg
    58801 gatacatttt caaatgctag taatgacata ggcttaaact cctttaatat aatcactgac
    58861 tacgcctggt agttcttctt tttcatagcc aaaatgggtc atggaggaaa cgaagtgttc
    58921 caattcttct tctgataatt gtttgcgtga ctgggcgatt agctccttat ccttagtcac
    58981 aaatcgtcca gttgttcgct tgctgtagac aaatccttct cgttcaaggt ctgataaggc
    59041 tctttgaatg gtattgggat tgacaccagc ctcgctagct agctccctca cggttggaag
    59101 ttgttgattg ggttccagtg tatgggaaac aatctgaagc ttgattttct ccataatctg
    59161 taaatagatg ggttttttgt tgtcaaatgt ccaggacatc ttagtctcct ttcttttacc
    59221 ttgttgtctt aattaaataa tacaacaaaa caaaaaactt gtcaagcaaa aagaagaatt
    59281 gttttgggaa tcgcttaaaa aatggtataa tgaaaagaaa acgataagga gggaaaggat
    59341 gcgttgtcca aaatgtgggg ctaccaagtc aagtgttatc gatagtcgcc aagcagaaga
    59401 agggaacacc attcgtagaa gacgtgagtg cgacgaatgc caacaccgtt ttacaaccta
    59461 cgaacgagta gaagaaagaa ccttagtggt tgttaaaaaa gatggcacac gggaacaatt
    59521 ctccagagat aaaatcttta atgggattat ccgctcagcc cagaaacgtc ctgtgtcaag
    59581 tgatgaaatc aacatggtag tcaatcgtat cgaacagaaa ctccgtggtc gaaatgaaaa
    59641 tgaaattcaa agtgaggaca ttggttcact cgtcatggag gagttggctg aattggacga
    59701 gattacctat gtacgttttg ctagtgtcta tcgtagtttt aaggatgtca gtgagttaga
    59761 gagcttgctc caacaaatca cccagtcctc taaaaagaaa aaggaaagat aaatgaagcc
    59821 aattgaccgt ttttcttatc taaagaataa tcgggtgtcg caagatacct catctctggt
    59881 acagtgctac ctcccgatta tcggtcagga ggcactgagc ctttatcttt atacaatcag
    59941 tttttgggat aatggtagaa aggaatatct tttttcaagc attctcaacc atctcaactt
    60001 tgggatggat agactgataa aatcattgaa aatcttatct gcttttaatc tcttgaccct
    60061 ctatcaaaag ggtgatgttt atcagctagc cctccatgct cctctatcta gtcaagactt
    60121 cttggggcat cctgtttatc gcagactctt agagaaaaag attggcgatg tagctgtgga
    60181 ggatttgaaa gttgaaagtg ctgatggtga agaaatacct gtctcactca atcaagtctt
    60241 tccagaattg gcagaactag gcagtcaaga agaccttggt ctcaagaaga aagtggccaa
    60301 tgattttgac ttggaacatt ttcgccagct gatggctcga gatggacttc gttttgcgga
    60361 tgagcagtcc gatgtcttaa acctctttgc tattgccgag gagaagaaat ggacttggtt
    60421 tgaaacctat caattggcca agtcaacagc tgtttcccag gttatttcaa ccaaacgcat
    60481 gcgtgaaaaa attgctcaaa aaccagtttc ctctgacttt agtcttaagg aagcgaccat
    60541 tatcaaagaa gctaaaagta aaactgccct gcagttctta gcagaaatca agcaaacacg
    60601 caagggaacc attacccaaa cagaaagaga actcttgcaa cagatggctg gcttgggctt
    60661 actggacgaa gtcatcaata ttattctctt attgaccttt aataaggtgg attcggcaaa
    60721 tatcaatgag aaatatgcca tgaaggtagc caatgactat gcctatcaaa agattcattc
    60781 ggcagaagag gccgttttac gaattcgtga tcgagggcag aaagcaaaaa cacaaaaaca
    60841 gaatcagact gccccagaaa aaaccaatgt tcctaaatgg agtaatcctg aatataagaa
    60901 tgaaaccagc gaggaaactc gtctggaact agaacgtaag aagcaagaac tattagctcg
    60961 attagaaaaa ggaggagatt agatggaaag tgtcggagac gtactcaaac gtcaacccag
    61021 ccgtttttat tatcaagatt tggtccagaa aatcatgaag gaccctgatg ttgcggcctt
    61081 tatccagcaa gaatccttaa ctccaaagga attaaatcgc agtatctcca agtttaatca
    61141 gtacatcacc gagcgtgaca agtttctccg tggggatacg gattatattg ccaaaggcta
    61201 caagccgatt ttggttaaga atcatggtta tgcagatgtt tcatatgaag aaactcctga
    61261 gctaatcgcg gctgaaaaag aagcggctat taagaaccgt ctcaagttaa tcaatctacc
    61321 agccagtctc aagaaagcta gtttggctca agttgacttg gatgatttgg ggcgcttacc
    61381 agtttttgaa aagctactag ccttcgtgga gcaatatcca gctattcgaa aaggtcttta
    61441 cttatatgga gactttggtg tgggtaaaag tttcatggtg gcggccttag ctcatgattt
    61501 atcagaaaaa cgtggtgttt catcaactct cctccactat cctagctttg tcattgatgt
    61561 caaaaatgct atcagtgatg gcaatgttaa gaccttggtg gatgagatta agctgtctga
    61621 ggtcctgatt ttagatgata ttggcgccga gcaatcaacc gtttgggtgc gtgacgaaat
    61681 cctgcaggtc attctccaat atcggatgca ggaaaattta ccgacctttt tcacatccaa
    61741 cttcaacttt gaagatttgg agaagcattt tgcgaaagtg aaacatggaa atgacgaaac
    61801 ctgggaagcc agacgggtca tggaacgcat ccgttatttg gctgaggaga ctcgtttaga
    61861 aggagtaaac cgtcgatgac agaaaccatt aaattgatga aggctcatac ttcagtgcgc
    61921 aggtttaaag agcaagaaat tccccaagta gacttaaatg agattttgac agcagcccag
    61981 atggcatcat cttggaagaa tttccaatcc tactctgtga ttgtggtacg aagtcaagag
    62041 aagaaagatg ccttgtatga attggtacct caagaagcca ttcgccagtc tgctgttttc
    62101 cttctctttg tcggagattt gaaccgagca gaaaagggag cccgacttca taccgacacc
    62161 ttccaacccc aaggtgtgga aggtctcttg attagttcgg tcgatgcagc tcttgctgga
    62221 caaaacgcct tgttggcagc tgaaagcttg ggctatggtg gtgtgattat cggtttggtt
    62281 cgatacaagt ctgaagaagt ggcagagctc tttaacctac ctgactacac ctattctgtc
    62341 tttgggatgg cactgggtgt gccaaatcaa catcatgata tgaaaccgag gctgccacta
    62401 gagaatgttg tctttgagga agaataccaa gaacagtcaa ctgaggcaat ccaagcttat
    62461 gaccgtgttc aggctgacta tgctggggcg cgtgcgacca caagctggag tcagcgccta
    62521 gcagaacagt ttggtcaagc tgaaccaagc tcaactagaa aaaatcttga acagaagaaa
    62581 ttattgtaga aagtgagaaa ttatggccct accaactatt gccattgtag gacgtcccaa
    62641 tgttgggaaa tcaaccctat ttaatcggat cgctggtgag cgaatctcca ttgtagaaga
    62701 tgtcgaagga gtgacacgtg accgtattta tgcaacgggt gagtggctca atcgttcttt
    62761 tagcatgatt gatacaggag gaattgatga tgtcgatgct cctttcatgg aacaaatcaa
    62821 gcaccaggca gaaattgcca tggaagaagc agatgttatc gtttttgtcg tgtctggtaa
    62881 ggaaggaatt actgatgcag acgaatacgt agctcgtaag ctttataaga cccacaaacc
    62941 agttatcctc gcagtcaaca aggtggacaa ccctgagatg agaaatgata tatatgattt
    63001 ctatgctctc ggtttgggtg aaccattgcc tatctcatct gtccatggaa tcggtacagg
    63061 ggatgtgcta gatgcgatcg tagaaaatct tccaaatgaa tatgaggaag aaaatccaga
    63121 tgtcattaag tttagcttga ttggtcgtcc taacgttgga aaatcaagct tgatcaatgc
    63181 tatcttggga gaagaccgtg ttattgctag tcctgttgct ggaacaactc gtgatgccat
    63241 tgatacccac tttacagata cagatggtca agagtttacc atgattgata cggctggtat
    63301 gcgtaagtct ggtaaggttt atgaaaatac tgagaaatac tctgttatgc gtgccatgcg
    63361 tgctattgac cgttcagatg tggtcttgat ggtcatcaat gcggaagaag gcattcgtga
    63421 gtacgacaag cgtatcgcag gatttgccca tgaagctggt aaagggatga ttatcgtggt
    63481 caacaagtgg gatacgcttg aaaaagataa ccacactatg aaaaactggg aagaagatat
    63541 ccgtgagcag ttccaatacc tgccttacgc accgattatc tttgtatcag ctttaaccaa
    63601 gcaacgtctc cacaaacttc ctgagatgat taagcaaatc agcgaaagtc aaaatacacg
    63661 tattccatca gctgtcttga acgatgtcat catggatgcc attgccatca acccaacacc
    63721 gacagacaaa ggaaaacgtc tcaagatttt ctatgcgacc caagtggcaa ccaaaccacc
    63781 aacctttgtc atctttgtca atgaagaaga actcatgcac ttttcttacc tgcgtttctt
    63841 ggaaaatcaa atccgcaagg cctttgtttt tgagggaaca ccgattcatc tcatcgcaag
    63901 aaaacgcaaa taaaaaagta gaatctggaa tgacagttcc ggattttttt gatataataa
    63961 aataatagaa aacgctatca aaagaaagag ggtgatgtcg gtgatggaac atttgtttaa
    64021 attcttactt ttagcaccgt acttttattt tgataactgg attgagaagg ccaacagaaa
    64081 tagtaagttt tttccaattt tttattattt ttactggttt tacatcccct tctattctct
    64141 ttttagcctt gcttggacag ttgtttcagt tctgtttttc aataccgtct tgagaaatgt
    64201 gacagatatc aagttatggg gcatttggtt tctttttatt ctgctagcta ttggtatgaa
    64261 ttggttaact tattcctgtt tcaaagaaat gtttcgcttg agacaggaac tagggaagtc
    64321 taagggtgga aggcattgat ttatatcggt ttctatttgt aggaggtggc cttatggtac
    64381 ctctaaaatc atttattaca cgctattcca aggtttatat tggtaataac atttaataga
    64441 acgaaactaa agaaaacaat ttaaaagagg aggtttgtga tggaacactt gtttaaaatg
    64501 ataatcttat taccctgttt ttactttttc agctggattg acaaggataa tagagaaagt
    64561 aaatttttcc caatttttta ctatttttac tggatttaca tcaccctcta tgctcttttt
    64621 agccttgctt ggacagtttt ttcagttctg tttttcaata tcgtcttgag aaatttgaca
    64681 gatatcaagt tatggggtat ttggctatta ttactactta tcgcttttgc tagtgattgg
    64741 ttagcctatg tctttttcaa aaaaatgctt gacttgagac gggaactagg gaagtctaaa
    64801 ggcggaaggc attgatttat actcttcgaa aatctcttca aaccacgtca acgtcgcctt
    64861 ggattatata tgtaactgac ttcgtcagtc ttatctacaa cctcaaagca gtgctttgag
    64921 caacctgcgg ctagtttcct agtttgctct ttgattttca ttgagtatta tattactttc
    64981 tatttgtagg aggtggctta tgaagattcc tctcttaact ttggcaaggc ataaatttgt
    65041 ttatgtcttg cttactttgc tttttcttgc tttggtttat cgtgacgttt tgatgactta
    65101 tttctttttt gatattcatg cgcccgatct agctaaattc gatggacaag caattaaaaa
    65161 tgacttatta aaatcagcat tagattttcg tattctccag ttcaatctag gtttttatca
    65221 atcatttatt attccaatca tcattgtttt gctaggtttt caatatattg agctgaaaaa
    65281 taaagtttta cgattgagta ttggaagaga agtgagttat caagggttaa aaagaaagtt
    65341 gactttgcaa gttgcaagta tcccttgttt gatatattta gtgactgtgc tgacaattgc
    65401 aattataacc tatttccttg ggactttttc tcctcttgga tggaattctc tattttctga
    65461 tggaagtggt ttacaaagac tcctagatgg agagataaaa agctatttgt tctttacttg
    65521 tgtcctacta atcggtattt tcatcaatgc aatctatttt ttacaaatag ttgattatgt
    65581 ggggaatgtg actcgttcgg caatcaccta tttgatgttt ctttggcttg gttctatgct
    65641 gctctatagt gccttgcctt actatatggt tcctatgacg agtttgatgc aagctagcta
    65701 tggggatgta agtttgatga aactctttac tccttatatc ctttatattg tcccttatat
    65761 ggtgcttgaa aaatatgaag ataatgttta agaattttaa caatatttgg ctaaagagaa
    65821 agatcgtttt actacttcgt atagttctga tgatgatttt gataaactat ctattgtcaa
    65881 cagcggttca aaagcaggat gctgttatct ttttcaagag agaattgatt tcaatctttt
    65941 cctataatga ctattctgaa gcgaatttag aaatccccaa actcttgtta aacctttcgc
    66001 ttttcatggt aggatggctc tctgtcattt tacttgaaag tgatttggca gaccattacc
    66061 atcacttgat tcgctatcaa tcaagctcct ttttcgatta tacaaggaaa cgattggttg
    66121 tcatttctaa attttttact caagatttgt ttgtctggtt tcttggttta cttccgctag
    66181 gaattcattt caaaacagtc gcacttttct ttttacttgc tcagttaatg atgttgtact
    66241 tactactgtc ttatctgata gcactgatta gtgcgggcgc tggtttttct ttttttctct
    66301 attttttggc atttgtggga caagaatgga tgatggatca tattgtaaca gtgtatttac
    66361 tactcttaag tttattagtt atgttgattg ttagtcgctt ggaagagaaa tttaagaaag
    66421 gataaacgat gagacttgaa attataaatg gacagaaaat ttatgggaaa agacctattt
    66481 taaatcagtt gaatttggtg tttcaatcag gaaaaattta tggacttaaa ggtgataatg
    66541 gatctggcaa gacggttctt ttaaagatac ttgctggtta tattaagctt gacaaaggaa
    66601 aagttcttca agatggtaaa gtttacgggg taaaaaatca ttatattcag gatgcaggaa
    66661 ttttaattga aaaagtcgag tttttatctc atttatccct gagagaaaat ttggaactgt
    66721 taaggtattt ttcatctaaa gttacggaaa aaagaattgc ctattggatt caatactatg
    66781 atttacagga atttgaagac attgaatacc gtcatttatc cttaggaaca aagcaaaaaa
    66841 tggccttgat tcaagccttt atttcctctc cttctatact ctttctcgat gaacctatga
    66901 atgctttgga tgagaagagt gtgaggttaa ccaaacaggt cattttatct tacctgaaaa
    66961 aagaaaatgg tctggttatc ctgacgtcgc acatatagga agatatttca gacctttgta
    67021 cagatgtatt agttgtcgaa aatggacata tacaaatgta aaggatatac aatcctagga
    67081 gatggcttat ggcacatcta aaatcattta ttacacgcta ttctaaggtc tatattggtt
    67141 tagttctgtt gatctggatt gctttcttct ttcttccttg gggcagtccg attctggggg
    67201 ggaagattga cctcttcagc atacagaaag tcttgctagt ttttggcatt ctgtccattt
    67261 tgatggcctt gctgtccaag aaagtcagtc tctttgtttt tggattgatc tgctgtcttt
    67321 ctctttggat taacttattt atcctatttg ctattttgcc gattcttggc aattaaagta
    67381 tcataaaagt cagagaggtt agcttggaaa ctaacctctt tttccttttc aaaatgggga
    67441 ttcttccttg aaaataatca gtaattgtgc taaaattaaa ggaacattct aaaatattcg
    67501 gaatttaaag taaggaaaaa catggctaat attttaaaaa caattatcga aaatgataaa
    67561 ggagaaatcc gtcgtctgga aaagatggct gacaaggttt tcaaatacga agaccaaatg
    67621 gctgctttga ctgacgacca actaaaagca aaaacagttg aatttaagga acgttatcaa
    67681 aatggagaat cactggattc attgctttac gaagcatttg cggttgtccg tgaaggtgcc
    67741 aaacgtgtcc taggtctctt cccttataag gttcaggtca tgggggggat tgttcttcac
    67801 catggtgacg tgccagagat gcgtacaggg gaagggaaaa ccttgactgc gaccatgccg
    67861 gtatacctca atgccctttc aggtaaaggg gttcacgtag ttacggttaa tgaatacctg
    67921 tcagaacgtg acgcgactga gatgggtgaa ttgtactctt ggcttggttt gtcagtaggg
    67981 attaacttgg ctaccaaatc tccaatggag aaaaaagaag cctatgagtg tgatattact
    68041 tactcaacta actcagaaat cggatttgac taccttcgtg acaacatggt cgttcgcgct
    68101 gaaaacatgg tacaacgtcc gcttaactat gccttggtcg atgaggttga ctctatcttg
    68161 attgacgagg ctcgtacacc tttgattgta tcaggtgcca atgcggttga aaccagtcag
    68221 ttgtatcaca tggcagacca ctatgtaaaa tctttgaaca aagatgacta catcatcgat
    68281 gtgcagtcta agactattgg tttgtctgat tcagggattg acagggctga aagctacttc
    68341 aaacttgaaa acctctatga catcgaaaac gtggctttga cccactttat cgataacgcc
    68401 cttcgtgcca actacatcat gcttctcgat attgactatg tggtgagcga agagcaagaa
    68461 atcttgattg tcgaccaatt tacaggtcgt accatggaag gtcgtcgtta ttctgatgga
    68521 ttgcaccaag ctattgaagc caaagaaggt gtgccaatcc aggatgaaac caagacatct
    68581 gcctcaatca cgtaccaaaa ccttttccgt atgtacaaga aattgtctgg tatgacgggt
    68641 acaggtaaga ctgaggaaga agaattccgt gaaatctaca acattcgtgt tattccaatc
    68701 ccaacaaacc gtcctgttca acgtattgac cactcagacc ttctttatgc aagtatagaa
    68761 tctaagttta aagcggttgt cgaagacgtt aaggctcgtt accaaaaggg tcaacctgtc
    68821 ttggttggta cagtagcggt tgaaactagt gactacattt ctaagaaatt ggttgcagct
    68881 ggtgttcctc acgaagtctt gaatgccaaa aaccactata gagaagccca aatcatcatg
    68941 aatgctggtc aacgtggtgc cgttaccatc gcaaccaaca tggcgggtcg tggtaccgac
    69001 atcaagcttg gtgaaggtgt tcgtgaactt ggaggacttt gtgttattgg tacagaacgt
    69061 catgaaagtc gtcgtatcga taaccagctt cgtggacgtt caggtcgtca aggagatcca
    69121 ggtgagtcac aattctacct atctcttgaa gatgatttga tgaaacgttt tggttctgaa
    69181 cgcttgaagg gaatctttga acgcttgaac atgtctgaag aggcgattga gtctcgcatg
    69241 ttgacgcgtc aggttgaagc agctcagaaa cgtgtcgaag gaaataacta cgatacccgt
    69301 aaacaagtcc ttcaatacga cgatgtcatg cgtgaacaac gtgagattat ctatactcaa
    69361 cgttatgatg tcattactgc agaccgtgac ttggcacctg aaattcaggc aatgatcaaa
    69421 cgcacgattg gtcgtgtcgt tgacggtcat gcgcgtgcta aacaagatga gaaactagag
    69481 gcaattttga acttcgctaa gtataacttg cttcctgaag attctattac gatggaagac
    69541 ttgtcaggct tgtctgataa ggccatcaag gaagagcttt tccaacgtgc cttgaaggtt
    69601 tacgatagtc aggtttcaaa actacgcgat gaagaagcag ttaaagaatt ccaaaaagtt
    69661 ttgattctac gagtggtgga taacaagtgg acagatcata tcgatgccct tgatcaattg
    69721 cgtaacgcgg ttggacttcg tggctatgct cagaacaacc ctgttgttga gtatcaggca
    69781 gaaggtttcc gtatgtttaa tgatatgatt ggttcgattg agtttgatgt gacacgcttg
    69841 atgatgaaag cacaaattca tgaacaagaa agaccacagg cagaacgtca tatcagtaca
    69901 acagcgactc gcaatatcgc tgctcaccaa gcaagtatgc cagaagattt ggatttgagc
    69961 cagattggac gcaatgaact ttgcccatgt ggttctggta agaaatttaa aaactgtcac
    70021 ggtaaaagac aataaaatga gatagtttag aggcggatat cttgtgaaaa gtaaattttt
    70081 actgggtatc cgtttgcttt ataaggagat gagttatggt atttacagca aaaagctcta
    70141 aaataaatat agaagaagtt cgtgccttgt caaaattaga aggtcaggct ttggagagga
    70201 aatcacagcg agatcaagag ctagaagcca ttatacgtgg agaagaccag cgaattctct
    70261 tggtaatcgg gccatgctca tctgacaacg aagaagctgt ccttgaatac gctaagcgtt
    70321 tggcagtcct acaagaagaa gtggcagatc gtatctttat ggttatgcgt gtttatactg
    70381 ccaaaccccg taccaacgga gatggctata agggcttgat tcaccagcct aacgcgacag
    70441 aagcgcctag tcttatcaat ggaatcaaag ccgttcgcca tcttcactat cgtgtcatca
    70501 cagaaacagg gatgacaact gctgatgaaa tgctttatcc tgaaaacctt ccgcttgtag
    70561 atgatttgat ttcttacatg gcagttggtg cccgttcagt tgaagaccag caacaccgct
    70621 ttgtggcaag tggggcagga ttttctactg gttttaaaaa tccaacctct ggaaatctca
    70681 atgtcatgtt taatgggatt tatgctgctc aaaacaaaca aagtttcctt ttcttaggaa
    70741 aagaagtaga aacaactggg aacccgcttt cacacgctat tcttcgtggt gctcttaatg
    70801 agtatggaaa aaatattccc aactactatt atgacaattt aattgatacc attgcccagt
    70861 atgagaaaat gggcttggaa aatcctttta tcatcattga taccaatcat gacaattctg
    70921 gtaagcagta tattgaacag atccgaattg tccgccagac cttgattaac cgtgcttgga
    70981 atgaaaaaat taagcagttc gttcgtggtt ttatgattga gtcttatctg gaagatggtc
    71041 gacaaaatga gccagaagta tttggtaagt ctatcacaga cccttgcctg ggttgggata
    71101 acacagaagc tcttgtcaga gaaatttaca aaacgttagg agaataagat ggcatttatt
    71161 gaaaaaggtc aagaaatcga tatggaagtc atcaaggctg aaacccaatt gtctgcggaa
    71221 gccttgagac tcaaggaaag ccgtgacagg gaattggcag atattatttc aggggaagat
    71281 gaccgtattc tcttggtgat tggtccttgc tcttctgata atgaagaggc ggtcttggaa
    71341 tatgctcgcc gtttatctgc cttgcaaaag aaggtagcgg ataagatttt catggtcatg
    71401 cgcgtgtata ctgctaagcc tcgtaccaat ggagacggct ataaaggatt agttcaccag
    71461 ccagatactt ctaaggctcc aagcctgatt aatggcttgc aggctgtgcg ccagttgcac
    71521 taccgcgtga ttacagagac tggtttgaca acggcagatg agatgcttta tccgtcaaat
    71581 ctgatcttgg tggatgactt ggtcagctac catgccgttg gagctcgttc tgtggaagac
    71641 caagagcacc gctttgtggc ttctgggatt gatgcaccag tagggatgaa aaatccaacc
    71701 tcaggaaatt tgggtgttat gtttaacgcc atctatgctg ctcaaaacaa gcaaaccttc
    71761 ctttatcatg ggcaggaagt tgagacatca ggtaatcctt tggcccatgt tatcctccgt
    71821 ggagcagtca acgagtatgg caattatatg ccgaattact actatgaaaa tctactccaa
    71881 gccattgaac gctatgaaac catgggactt gaaaatcctt ttatcctcat tgacaccaac
    71941 catgataact caggcaagca atatatggag cagattcgaa ttgttcgcca gaccttgcag
    72001 aatcgtgatt ggaatgagaa aattaaaaag acggttcgag gatttatgat tgaatcttac
    72061 ctagcagatg gtcgtcaaaa ccaaccagag atctttggtt gctctattac tgacccttgc
    72121 ctaggttggg aaaatacaga ggccttggta gaagagattt atgttacctt gacaaaataa
    72181 gtgaaaagga tggagttggg gaatctcaac tccttttgat gagaatgata gttggacacg
    72241 gaattgacat cgaagaattg gcttcgatag aaagcgcagt tacacgacat gaaggatttg
    72301 ctaagcgtgt actgaccgct caggaaatgg agcgcttcac cagtctcaaa ggacgcaggc
    72361 aaatagaata tttagctggt cgctggtcgg ctaaggaggc cttttccaag gctatgggaa
    72421 cgggcattag caagctcggt tttcaggatt tggaagtctt gaacaatgaa cgtggggcgc
    72481 cttattttag tcaggcacca ttttcaggaa agatttggct gtctatcagc cacaccgatc
    72541 agtttgtgac agccagtgtc attttggagg aaaatcatga aagctagtcc acatagacca
    72601 accaaggctc tgattcatct gggagctatt cgacaaaata ttcagcaaat gggggctcat
    72661 atccctcaag gaacgctcaa gttggctgtg gttaaggcca atgcttatgg tcatggagct
    72721 gttgccgttg ccaaggcaat tcaagatgat gttgatggct tttgcgtttc caatatcgat
    72781 gaagccattg aactcagaca agctggactc agcaagccaa tcctcatttt aggagtttct
    72841 gaaatcgaag ctgttgctct agctaaagaa tatgacttca ccttgacagt ggctggactg
    72901 gagtggattc aagcactctt agataaggaa gtggacctaa ctggattgac agtccacctc
    72961 aagattgatt cagggatggg acggattggt tttagagagg ccagtgaggt tgagcaggct
    73021 caagatttgc tccaacaaca cgatgttcgt gttgaaggaa tctttaccca ctttgctact
    73081 gctgatgagg aatcagatga ctattttaat gcccagttag aacggtttaa aactatttta
    73141 gctagtatga aggaagttcc agagctggtt catgctagca attctgcaac gactctttgg
    73201 catgtagaga ctattttcaa tgcggttcgt atgggagatg ccatgtatgg cctcaatcca
    73261 agtggagcgg tcttggattt gccttatgat ttgataccgg ccttgacctt ggagtctgct
    73321 ctggttcatg tcaagacagt tccagctgga gcttgcatgg gctatggagc aacttatcaa
    73381 gcggatagcg agcaagtcat cgcgaccgtg ccaatcgggt atgcagatgg atggacaaga
    73441 gacatgcaaa atttctctgt cttggtagat ggtcaagctt gcccaattgt cggcagggtt
    73501 tcgatggacc aaatcactat tcgattgcct aagccttacc cactcggaac caaggtaacc
    73561 ttgattggct ccaatgggga taaggaaatc actgcaactc aggtagcgac ctaccgcgta
    73621 accattaact atgaggtggt ttgcctcctc agcgaccgta ttccgagaga atattattag
    73681 aaaagaaagg agtggagcat gaatctacat caacccttgc atgtcttgcc tggtgtggga
    73741 ccaaagtcag cagaaaaata cgccaaacta ggaattgaaa acttgcaaga tctcttgctc
    73801 tactttcctt tccgttatga agacttcaaa accaagcagg tgctggagct ggaagacggt
    73861 gagaaggcag ttctttctgg tcaggtagtg actcctgcta gtgtccagta ttatggtttc
    73921 aagcgcaatc gcctgcgttt tagtctcaag cagggagagg tcgtttttgc ggcgaatttc
    73981 tttaaccagc cctatctggc tgataaaata gagttgggag caacccttgc tgtctttgga
    74041 aaatgggacc gcgctaaggc tagtctgact gggatgaagg ttctggctca ggtagaagat
    74101 gacctccagc ctgtctatcg tctggctcag ggaatcagtc aggccagtct ggttaaggtc
    74161 atcaagacgg cttttgatca gggactggac ctcttgatag aagaaaatct gccccagtct
    74221 ttactagaca aatacaaact catgtcccgt tgtcaggcag tccgagctat gcattttcca
    74281 aaggatttgg cagaatacaa gcaggctctt cgccgtatca agtttgagga actcttttat
    74341 ttccaaatgc agttgcagac gctcaagtct gaaaatagag ttcagggaag tggtctggtt
    74401 ctgaattggt ctcaggaaaa agtgacagca gttaaagcaa gtcttccttt tgccctgacc
    74461 caagctcagg aaaagagttt gcaggaaatt ttaactgata tgaagtccga ccaccacatg
    74521 aatcgtctcc tacaagggga tgtggggagt ggaaaaacgg tagtcgctgg cttggccatg
    74581 tttgcggcag tgacagcagg ttatcaggct gccctaatgg taccaacaga aatcctcgca
    74641 gagcaacact ttgagagttt acagaacctt tttcccaatt tgaaactggc tctcttgaca
    74701 ggttccttga aagctgcaga aaagagagaa gtcttggaga ccattgccaa gggtgaggct
    74761 gatttgatta taggaactca cgctctgata caatatgggg tggagtatgc tcgtcttggt
    74821 ttgattatta tcgatgagca gcaccgtttt ggtgtagggc aaaggcgtat tttacgggaa
    74881 aaaggtgaca atccagatgt cctcatgatg acggcgactc ccattccacg gacgcttgcc
    74941 atcacagcct ttggagatat ggatgtttcc attatcgacc agatgccagc aggtcggaag
    75001 cctattgtga cgcgctggat caaacatgag caactacctc aggtcttgac ttggttagag
    75061 ggggaaattc aaaaaggttc ccaagcctat gtcatctctc ctttgattga agaatcagaa
    75121 gctctagatt tgaaaaatgc cattgcctta tcagaggagt tgacgactca ttttgcaggc
    75181 aaggcagagg tggctcttct acatggtagg atgaagagtg acgaaaaaga ccagatcatg
    75241 caggatttca aggagagaaa gacggatatt ctggtttcga cgacggttat tgaggttggg
    75301 gtcaacgttc ccaatgcgac tgtcatgatt atcatggatg ccgatcgctt cggtctcagt
    75361 caacttcacc agcttagagg tcgtgtcggt cggggggaca agcagtccta cgctgttctc
    75421 gttgccaatc ccaagacgga ttctgggaaa gaccgcatgc gcatcatgac agaaacgacc
    75481 aatggatttg tccttgcgga ggaagatttg aaaatgcgtg gttctggtga gatttttgga
    75541 accagacagt caggacttcc agagttccaa gtggctgata ttatcgaaga ttttccgatt
    75601 ttagaagaag caagaaaggt tgctagctac attagttcta tagaagcttg gcaagaagat
    75661 ccagagtggc gcatgattgc ccttcatctg gaaaagaaag aacatctgga ttaagctttc
    75721 tctaaggaaa acttaagcta gtttttagat ttggctctta tactaaagtc atcaaaaaga
    75781 aacgaggact ctcatatgac agtaacgatt aaagtaaatt accaaaccac tttccaaaag
    75841 aaggaagcaa aaaactagta taaacagaag agagagcgaa atgctctttt ttcgtttcta
    75901 aaactacttt cagcccatca tcctaaaagt aaagaatcta aattcacttt ctatttatcc
    75961 ttctttcttg cattgattac atagatatgc tacagttgtg gtaacgatta caaaataaaa
    76021 ggagcatgct atgaaaaatc cagctttgct agaagaaatt aagacctata gaggaaggga
    76081 tgaggttccg gaagactttg atgctttctg ggatggggaa gtgaaaaatg tttccacgct
    76141 tccatcctac cacttggagg aaaaagattt ccacattcct caagtcaagt actatgagtt
    76201 aacatttgaa ggaagcaagg aaggaaaggt ctatgcacgc attgttcttc caaagagtga
    76261 ggagaaggtt ccactaatct tccattttca tggttatatg ggacgtggct gggactgggc
    76321 cgacatgctg ggcttcaccg tagctggtta cggtgttgtt tccatggatg tgcggggcca
    76381 gtcaggttac tcacaagacg gcttgcgttc tcctttagga aataccgtga aggggcatat
    76441 tatccgtggt gctgtggaag gtcgggacca cctcttttat aaggatgttt atctggatat
    76501 ttaccagttg gtcgaaattg ttgctagtct gtctcaggtt gatgagaagc gtctttctag
    76561 ctatggtgcc tcacaaggag gggctctagc cctagttgca gcagcgctca atcctcgaat
    76621 tcagaaaaca gttgctgttt atcccttctt gtcagacttc agacgggtga ttgagattgg
    76681 taatactagc gaggcttacg acgaactttt ccgttatttc aagtttcacg accccttcca
    76741 tgaaacagag gaggaaatca tggcgaccct tgcctatatc gatgtcaaaa atcttgccca
    76801 tcgtatccaa ggtgaggtta agatgattac gggcttggac gacgatgttt gctatcccat
    76861 tacccagttt gcgatttata atcgtctgac ctgcgataaa acctatcgta tcatgcctga
    76921 gtatgctcac gaagccatga atgtatttgt caatgaccaa gtctacaact ggctctgtgg
    76981 aagtgagatt ccttttaaat atttaaaata aggagtcgac tctaagcaca aaatcttaaa
    77041 aattacaaac acgcatagta tcaggggctt aagaaaactt gatactatgc gttttatcat
    77101 ggaaatatag taaaatgaaa caagaacagg acaa
//
LOCUS       DAVKJD010000002        75536 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKJD010000002 DAVKJD010000000
VERSION     DAVKJD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 75536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.002"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            119..2011
                     /gene="pepO"
                     /locus_tag="VW157_000085"
     CDS             119..2011
                     /gene="pepO"
                     /locus_tag="VW157_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9551474.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(2134..2763)
                     /locus_tag="VW157_000086"
     CDS             complement(2134..2763)
                     /locus_tag="VW157_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9551475.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(3103..3345)
                     /locus_tag="VW157_000087"
     CDS             complement(3103..3345)
                     /locus_tag="VW157_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551476.1"
                     /translation="MMRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            3756..5978
                     /locus_tag="VW157_000088"
     CDS             3756..5978
                     /locus_tag="VW157_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9551477.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            6008..6451
                     /locus_tag="VW157_000089"
     CDS             6008..6451
                     /locus_tag="VW157_000089"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9551478.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(6490..6904)
                     /locus_tag="VW157_000090"
                     /pseudo
     CDS             complement(6490..6904)
                     /locus_tag="VW157_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6923..7336)
                     /locus_tag="VW157_000091"
     CDS             complement(6923..7336)
                     /locus_tag="VW157_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551479.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMS
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            complement(7438..8115)
                     /locus_tag="VW157_000092"
     CDS             complement(7438..8115)
                     /locus_tag="VW157_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU9551480.1"
                     /translation="MVVGIAIASGYLNSRLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTLLFGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYEGKMDLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVRYIE
                     GNSNKEY"
     gene            complement(8314..8964)
                     /locus_tag="VW157_000093"
     CDS             complement(8314..8964)
                     /locus_tag="VW157_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9551481.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     NFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            9076..9918
                     /locus_tag="VW157_000094"
     CDS             9076..9918
                     /locus_tag="VW157_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9551482.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            9994..10431
                     /locus_tag="VW157_000095"
     CDS             9994..10431
                     /locus_tag="VW157_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9551483.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            10499..10801
                     /locus_tag="VW157_000096"
     CDS             10499..10801
                     /locus_tag="VW157_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551484.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(10892..11965)
                     /locus_tag="VW157_000097"
     CDS             complement(10892..11965)
                     /locus_tag="VW157_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9551485.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            12121..12798
                     /locus_tag="VW157_000098"
     CDS             12121..12798
                     /locus_tag="VW157_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9551486.1"
                     /translation="MHKILLVEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            12791..13765
                     /locus_tag="VW157_000099"
     CDS             12791..13765
                     /locus_tag="VW157_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9551487.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(13814..15757)
                     /gene="thrS"
                     /locus_tag="VW157_000100"
     CDS             complement(13814..15757)
                     /gene="thrS"
                     /locus_tag="VW157_000100"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9551488.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(15815..16138)
                     /locus_tag="VW157_000101"
     CDS             complement(15815..16138)
                     /locus_tag="VW157_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEU9551489.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            complement(16569..16769)
                     /locus_tag="VW157_000102"
     CDS             complement(16569..16769)
                     /locus_tag="VW157_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551490.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIISLLYSYRFRFSL"
     gene            17008..17637
                     /locus_tag="VW157_000103"
     CDS             17008..17637
                     /locus_tag="VW157_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9551491.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKDQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            17778..18047
                     /gene="rpsO"
                     /locus_tag="VW157_000104"
     CDS             17778..18047
                     /gene="rpsO"
                     /locus_tag="VW157_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9551492.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            18780..19394
                     /locus_tag="VW157_000105"
     CDS             18780..19394
                     /locus_tag="VW157_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9551493.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(19687..20436)
                     /locus_tag="VW157_000106"
     CDS             complement(19687..20436)
                     /locus_tag="VW157_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9551494.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            20569..22905
                     /locus_tag="VW157_000107"
     CDS             20569..22905
                     /locus_tag="VW157_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU9551495.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNLAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEIATLTGQTLPVYGVMMLVFTVIFILTSRYQEKK"
     gene            23007..23684
                     /locus_tag="VW157_000108"
     CDS             23007..23684
                     /locus_tag="VW157_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9551496.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEEIDQVSVITIAGMGGCLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQNHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVPHVSK"
     gene            23671..24468
                     /locus_tag="VW157_000109"
     CDS             23671..24468
                     /locus_tag="VW157_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9551497.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSCGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELGILPSQASTNPFRHI"
     gene            24485..25588
                     /locus_tag="VW157_000110"
     CDS             24485..25588
                     /locus_tag="VW157_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9551498.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            25595..25786
                     /locus_tag="VW157_000111"
     CDS             25595..25786
                     /locus_tag="VW157_000111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551499.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVYRTDEVSSKHCFEVVYRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            25889..26908
                     /gene="galE"
                     /locus_tag="VW157_000112"
     CDS             25889..26908
                     /gene="galE"
                     /locus_tag="VW157_000112"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9551500.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            26910..27875
                     /locus_tag="VW157_000113"
     CDS             26910..27875
                     /locus_tag="VW157_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9551501.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFSAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(27926..28132)
                     /locus_tag="VW157_000114"
     CDS             complement(27926..28132)
                     /locus_tag="VW157_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9551502.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            28184..28660
                     /locus_tag="VW157_000115"
     CDS             28184..28660
                     /locus_tag="VW157_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9551503.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            28669..29340
                     /locus_tag="VW157_000116"
     CDS             28669..29340
                     /locus_tag="VW157_000116"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9551504.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            29470..29808
                     /locus_tag="VW157_000117"
     CDS             29470..29808
                     /locus_tag="VW157_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9551505.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            29811..30440
                     /locus_tag="VW157_000118"
     CDS             29811..30440
                     /locus_tag="VW157_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9551506.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            30680..31831
                     /locus_tag="VW157_000119"
     CDS             30680..31831
                     /locus_tag="VW157_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9551507.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            32144..32893
                     /gene="truA"
                     /locus_tag="VW157_000120"
     CDS             32144..32893
                     /gene="truA"
                     /locus_tag="VW157_000120"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9551508.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVAADFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            32883..33656
                     /locus_tag="VW157_000121"
     CDS             32883..33656
                     /locus_tag="VW157_000121"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9551509.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSDLCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LRDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            33634..34095
                     /locus_tag="VW157_000122"
     CDS             33634..34095
                     /locus_tag="VW157_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9551510.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            complement(34354..34557)
                     /locus_tag="VW157_000123"
     CDS             complement(34354..34557)
                     /locus_tag="VW157_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9551511.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(34550..35632)
                     /locus_tag="VW157_000124"
     CDS             complement(34550..35632)
                     /locus_tag="VW157_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9551512.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(35758..36540)
                     /locus_tag="VW157_000125"
     CDS             complement(35758..36540)
                     /locus_tag="VW157_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9551513.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(36540..37883)
                     /locus_tag="VW157_000126"
     CDS             complement(36540..37883)
                     /locus_tag="VW157_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9551514.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(37982..39298)
                     /locus_tag="VW157_000127"
     CDS             complement(37982..39298)
                     /locus_tag="VW157_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9551515.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            39491..40717
                     /locus_tag="VW157_000128"
     CDS             39491..40717
                     /locus_tag="VW157_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9551516.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            40916..42490
                     /locus_tag="VW157_000129"
     CDS             40916..42490
                     /locus_tag="VW157_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9551517.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVVLKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            42755..43543
                     /gene="codY"
                     /locus_tag="VW157_000130"
     CDS             42755..43543
                     /gene="codY"
                     /locus_tag="VW157_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9551518.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            43543..44118
                     /locus_tag="VW157_000131"
     CDS             43543..44118
                     /locus_tag="VW157_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9551519.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            44200..44285
                     /locus_tag="VW157_000132"
     tRNA            44200..44285
                     /locus_tag="VW157_000132"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44234..44236,aa:Leu,seq:aag)
     gene            44568..45698
                     /gene="ugpC"
                     /locus_tag="VW157_000133"
     CDS             44568..45698
                     /gene="ugpC"
                     /locus_tag="VW157_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9551520.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            46209..46967
                     /locus_tag="VW157_000134"
     CDS             46209..46967
                     /locus_tag="VW157_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9551521.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            47054..47566
                     /locus_tag="VW157_000135"
     CDS             47054..47566
                     /locus_tag="VW157_000135"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9551522.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            47748..48692
                     /locus_tag="VW157_000136"
     CDS             47748..48692
                     /locus_tag="VW157_000136"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9551523.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYRYL"
     gene            48701..49378
                     /locus_tag="VW157_000137"
     CDS             48701..49378
                     /locus_tag="VW157_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9551524.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEISPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            49476..50234
                     /locus_tag="VW157_000138"
     CDS             49476..50234
                     /locus_tag="VW157_000138"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9551525.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            50272..51777
                     /gene="lytC"
                     /locus_tag="VW157_000139"
     CDS             50272..51777
                     /gene="lytC"
                     /locus_tag="VW157_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU9551526.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWYGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            52273..52791
                     /locus_tag="VW157_000140"
     CDS             52273..52791
                     /locus_tag="VW157_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9551527.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            52921..53427
                     /locus_tag="VW157_000141"
     CDS             52921..53427
                     /locus_tag="VW157_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9551528.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            53427..53597
                     /locus_tag="VW157_000142"
     CDS             53427..53597
                     /locus_tag="VW157_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551529.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            53629..54861
                     /gene="clpX"
                     /locus_tag="VW157_000143"
     CDS             53629..54861
                     /gene="clpX"
                     /locus_tag="VW157_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9551530.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            54870..55457
                     /locus_tag="VW157_000144"
     CDS             54870..55457
                     /locus_tag="VW157_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9551531.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            55468..55848
                     /locus_tag="VW157_000145"
     CDS             55468..55848
                     /locus_tag="VW157_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9551532.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            55900..56790
                     /gene="rapZ"
                     /locus_tag="VW157_000146"
     CDS             55900..56790
                     /gene="rapZ"
                     /locus_tag="VW157_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9551533.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            56787..57764
                     /locus_tag="VW157_000147"
     CDS             56787..57764
                     /locus_tag="VW157_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9551534.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            57761..58672
                     /gene="whiA"
                     /locus_tag="VW157_000148"
     CDS             57761..58672
                     /gene="whiA"
                     /locus_tag="VW157_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9551535.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(58706..59674)
                     /locus_tag="VW157_000149"
     CDS             complement(58706..59674)
                     /locus_tag="VW157_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9551536.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            59810..60667
                     /locus_tag="VW157_000150"
     CDS             59810..60667
                     /locus_tag="VW157_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9551537.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            60654..61433
                     /locus_tag="VW157_000151"
     CDS             60654..61433
                     /locus_tag="VW157_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU9551538.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            61457..62809
                     /locus_tag="VW157_000152"
     CDS             61457..62809
                     /locus_tag="VW157_000152"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9551539.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKKTGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            62883..63257
                     /locus_tag="VW157_000153"
     CDS             62883..63257
                     /locus_tag="VW157_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9551540.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            63259..64107
                     /locus_tag="VW157_000154"
     CDS             63259..64107
                     /locus_tag="VW157_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9551541.1"
                     /translation="MKLAVFTDSSAYLSAETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            64384..65151
                     /locus_tag="VW157_000155"
     CDS             64384..65151
                     /locus_tag="VW157_000155"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9551542.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            65163..66347
                     /locus_tag="VW157_000156"
     CDS             65163..66347
                     /locus_tag="VW157_000156"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9551543.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLENISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQNKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQARGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            66344..68215
                     /locus_tag="VW157_000157"
     CDS             66344..68215
                     /locus_tag="VW157_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9551544.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDATLLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLEPIAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(68358..69542)
                     /gene="mntE"
                     /locus_tag="VW157_000158"
     CDS             complement(68358..69542)
                     /gene="mntE"
                     /locus_tag="VW157_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9551545.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            69846..72542
                     /locus_tag="VW157_000159"
     CDS             69846..72542
                     /locus_tag="VW157_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9551546.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALITFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            72555..73346
                     /gene="yghU"
                     /locus_tag="VW157_000160"
     CDS             72555..73346
                     /gene="yghU"
                     /locus_tag="VW157_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU9551547.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            73462..73872
                     /locus_tag="VW157_000161"
     CDS             73462..73872
                     /locus_tag="VW157_000161"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9551548.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            73884..75479
                     /locus_tag="VW157_000162"
     CDS             73884..75479
                     /locus_tag="VW157_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551549.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRVYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYVLVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT        21924 a        14218 c        17325 g        22069 t
ORIGIN      
        1 ttttatcatg gaaaggtttt ttataatagc taaaaaataa tagttaaatg gttattaatt
       61 gcattcccta gtgatttttg ttaagataaa tgcaaataca aatgaaagcg agaacaagat
      121 gacacgttat caagatgatt tttatgatgc tatcaatgga gaatggcaac agacagctga
      181 aatcccagca gataagtctc aaacaggagg ttttgttgat ttagaccagg aaattgaaga
      241 cctgatgctg gcgacaacag acaagtggtt agcaggtgaa gaagtgcctg aggatgctat
      301 cttggaaaac tttgtcaaat accaccgcct agttcgtgat tttgacaaga gagaagctga
      361 cggtatcaca cctgtcttac cactccttaa agaattccaa gaattggaga cttttgcgga
      421 ttttacagct aaactagcag agtttgagct tgcaggaaaa ccaaacttcc ttccttttgg
      481 tgtatcgcca gactttatgg atgctagaat caatgttcta tgggctagcg ctccaagcac
      541 aatcttgcca gatacgacct actatgcaga agaacatcct cagcgcgaag aactcttgac
      601 tctttggaaa gaaagcagcg caaatctcct caaggcttat gatttctctg atgaagaaat
      661 tgaagacttg ctagaaaaaa gacttgaatt ggaccgccga gttgcggcag tggtgctctc
      721 taatgaagaa agttcagaat atgctaaact ctatcatcca tattcttacg aagatttcaa
      781 gaaattcgcg cctgccctac ctttggatga cttcttcaaa gcagttattg ggcaattacc
      841 agacaaggtt attgtagacg aggaacgttt ctggcaagca gcagagcaat tctacagtga
      901 ggaagcctgg tctctcctta aagcaacctt gattttgagt gttgtcaatc tttcaaccag
      961 ctatttaaca gaggatatcc gtgttttgtc tggtgcctac agccgtgccc tttctggagt
     1021 tccagaggca aaagataagg tcaaagcagc ttatcatcta gcacaggaac ctttcaagca
     1081 agccctgggg ctttggtacg cccgtgagaa gttctctcca gaagccaagg cggatgtgga
     1141 gaaaaaagtg gcaaccatga ttgatgtcta taaggagcgt ctgcttaaga atgactggct
     1201 cactccagaa acctgtaaac aggctatcgt gaagctcaat gtgatcaaac cttatattgg
     1261 ctatccagaa gaattgcctg cacgttacaa ggataaggta gtgaatgaaa ctgccagtct
     1321 ttttgagaat gctctagcct ttgcgcgtgt ggaaatcaag cacagttgga gtaagtggaa
     1381 ccagcctgta gactataagg aatggggcat gcctgctcat atggtcaatg cctactacaa
     1441 tcctcagaag aacctgattg tctttccagc ggccatttta caggcgcctt tctatgactt
     1501 gcatcagtca tcttctgcta actacggtgg tattggggca gtgattgccc atgaaatttc
     1561 ccacgccttt gatactaacg gggcttcctt tgacgaaaat ggtagcctca aggattggtg
     1621 gacagagagc gactatgctg ccttcaagga gaaaacacaa aaagtcattg accaatttga
     1681 tggacaggat tcttatggag caaccattaa cggtaaattg actgtatcag aaaacgtggc
     1741 tgacttggga ggaatcgcag cagcgcttga agcagctaag agagaagcag acttctcagc
     1801 agaagagttc ttctacaact tcggtcgcat ctggcgcatg aaaggtcgac cagaatttat
     1861 gaaacttttg gctagcgtcg atgtgcacgc accagccaaa ctccgtgtca atgtgcaagt
     1921 accaaacttc gacgatttct ttacaaccta tgatgtcaaa gaaggagacg gaatgtggcg
     1981 ttcaccagag gagcgcgtga ttatttggta atacaaaaaa tctagtcatc agaaaaaagg
     2041 catccaatca cgtgtgaaaa ccttgatagg atgccttttg taatagaaag atttgctgga
     2101 tagtttactt aaatttcgat tgtcatcatc ttgctagaaa aagggattga aggccttttc
     2161 gtgagcgata gtagtagctg gaccatgtcc tggatagaca tcgtagtttg gtagggtgaa
     2221 gagttgggtc tggatactat gaaggagttg ctccatgcta ccagtcggaa ggtcggtccg
     2281 tccgatagtt tcgcggaata gagcatctcc cgtcaagact aggtgagcat caggaaagac
     2341 tagggaaaca ccaccgatag agtgccctgg ggtcggtaga accttaaaac gaaattcctc
     2401 tagttggtat tcttcgtgaa agacaaaggt gtgttctgca ggttttgtga ccacatctgc
     2461 catatcatcg tggcgaggga gaccggagag attatcgaca ggagtgtaga gccagctggc
     2521 ttcgctctct gcgatataga caggaggatt gccaaacgtc tcgcgaacca agtccagact
     2581 catgatatgg tcataatggg cgtgggtcaa gagaatagca cagatcggtt tgttgatctt
     2641 ctcgattgtc tgacgaatgg cttcccaatg actaccagga tcgacgacga tgaggtgctt
     2701 ttcgccttct agataatagg tattttcata ggcaacagga ttcacggttt tatggatttt
     2761 catactagct ccaatctcaa agaatgtact aatactcttc gaaaatctct tcaaaccgcg
     2821 tcaactttat ctgcaacctc aaagcagtgc tttgaacagc ctgcggctag ctttctagtt
     2881 tgctctttga ttttcattga gtataaatta agtatagcac agttagggag aataggtaag
     2941 gatttaaaat gaacttaaaa accagcatga ctagatgaag acaggctggt tatcaattta
     3001 tcaatattgg aaaggtgtca attgcccttc ataggttgca taaactccgt cattagcttg
     3061 tttgatagac gtgatgttta gagtgctacc gtagttggct tctcatttgt cgctatattc
     3121 actataagtt atcctactgg gcaggttctg gtcgttagca tagtattgaa cgagtttttt
     3181 atagctttgc tggctgaata ggaacaggca gcaaactcct aacattagga gagcaaagat
     3241 agaaatagca gttttttcat aggtattctc cttataaact aaaggataga agaaagtatc
     3301 ttgaggattt ttatactctt caaaaatctc ttcaaaccgc atcaacgtcg ccttgccgta
     3361 tatatgtgac tgactttgtc agtcttatct acaacctcaa agcagtgctt tgaacagcct
     3421 gcggctagtt tcctagtttg ctcttggatt ttcattgagt attaattcca aaatccagtc
     3481 aaggtaccag tgtatgtgat acgagttcca ccgtttatct ttcgtatctt caatggaatt
     3541 ggggaagtca gaaagtcttt gggttacaat atggtttccg ttcttctatc tctccttcta
     3601 tcacctaagt ttaaaatcta acttcgtagt atattatgga ccaataattc agaattatca
     3661 agcaattttg aattattttt ttcttattct gttccaatct agttttcata ttcttaaaaa
     3721 agtgataaaa tggtaggaag aattggagag tagagatgcc gaaagaagtg aatttaacag
     3781 gcgaagaagt tgtcgcttta accaaagaat atttaacgga agaggatgtt cattttgtcc
     3841 ataaggcctt ggtctatgct gttgaatgcc acagtggtca atatcgcaaa tcaggcgagc
     3901 cttatatcat tcaccctatc caagtggcag gtattttagc taagctaaag ctggatgctg
     3961 taacagtagc ttgtggattc ttgcatgatg tggtggaaga tacagatgcg accttggacg
     4021 atttggaaag agagtttggt cctgatgtgc gggtgattgt tgacggagtt accaagcttg
     4081 gcaaggtcga gtacaaatca atcgaggagc aattagcgga aaatcatcgc aagatgctca
     4141 tggccatgtc tgaggacatc cgcgttattt tggtcaaact gtctgaccgc ttgcacaata
     4201 tgcggaccct gaaacatctt cgaaaagaca agcaggagcg tatttccaaa gaaaccatgg
     4261 aaatctatgc cccacttgcc catcgtttgg ggatttccag tgtcaaatgg gaattagaag
     4321 acttgtcttt ccgttatctc aatccaacgg agttttacaa gattacccat atgatgaagg
     4381 aaaagcgcag ggagcgtgag gccttggtgg atgaggtagt cacaaaatta gaggagtata
     4441 cgacagaacg tcacttgaaa gggaagattt atggtcgtcc caagcatatt tactcaattt
     4501 tccgcaaaat gcaggacaag agaaaacggt ttgaggaaat ctatgatctg attgctattc
     4561 gttgtatttt agatacccaa agtgatgttt atgccatgct tggttacgtg catgaatttt
     4621 ggaaaccgat gccaggtcgc ttcaaagact atatcgccaa ccgcaaggcc aatggttatc
     4681 agtctatcca tacgactgtt tatggaccaa aagggccgat tgaattccag attcgaacca
     4741 aggaaatgca cgaggtggct gagtacgggg ttgcggctca ctgggcttat aagaaaggta
     4801 taaaggggca agttaacagc aaggaatcag ctattggaat gaactggatc aaggagatga
     4861 tggagctcca agaccaggct gatgatgcta aggaatttgt ggactctgtt aaggaaaact
     4921 atttggctga ggagatttac gtttttaccc cagatggagc tgtccgttcc cttcccaaag
     4981 attcaggacc gattgatttt gcctacgaaa tccataccaa ggtcggtgaa aaagcaactg
     5041 gtgccaaggt caatggccgc atggttccac tgacaaccaa gttaaagaca ggggatcagg
     5101 ttgaaattat cgccaacccg aactcctttg gacctagccg tgactggctc aatatggtca
     5161 agactagcaa ggcgcgcaat aagattcgcc agttctttaa aaaccaagat aaggaattgt
     5221 ctgtcaacaa gggtcgtgag atgctgatgg ctcagttcca agaaaatggc tatgtggcaa
     5281 ataaatttat ggacaagcgc cacatggatc aagttctgca aaagaccagt tacaagacag
     5341 aagactccct ctttgcggcc attggttttg gggaaatcgg tgcgattacc gtctttaacc
     5401 gtctgactga aaaggaacgc cgtgaggaag agcgtgccaa ggccaaggct gaggcagagg
     5461 agcttgtcaa aggtggcgag gtcaaggttg aaaataaaga aactctcaag gtcaagcatg
     5521 aggggggagt ggttattgaa ggtgcttctg gtctcctagt gcggattgct aagtgttgta
     5581 accccgtgcc tggtgacgat attgttggct acattaccaa gggtcgtggt gtggctattc
     5641 accgtgtgga ctgtatgaac ctgcgtgccc aagaaaacta cgagcaacgt ctccttgatg
     5701 tggaatggga agaccagtac tctagctcaa ataaggagta tatggcccat atcgatatct
     5761 acggtctcaa ccgtacagga ctgttgaacg atgtactgca agttctttca aatacaacca
     5821 agaatatttc aacggtcaat gcccaaccaa ccaaggatat gaagtttgct aatatccatg
     5881 tgtccttcgg tattgccaac ctctctacac tgactacggt tgtcgataaa attaagagtg
     5941 tgccagaagt ttactctgtc aaacggacca acggctagtt gtcctagctc ttactagaaa
     6001 ggctattatg aaaatcatta tccaacgggt taaaaaagcc caagtgagta tagaaggcca
     6061 gattcaggga aaaatcaatc agggactttt attgctagtt ggtgttggac cagaggacca
     6121 agaggaagat ttggactatg ctgtgagaaa actggtcaat atgcggattt tttcagacgc
     6181 agaaggcaag atgaacctgt ctgtcaaaga tattgaagga gaaatcctct ctatttctca
     6241 gtttaccctc tttgcggata ctaagaaagg caatcgtcca gcctttacag gggcagctaa
     6301 acctgatatg gcatcagact tctatgatgc tttcaatcaa aaattagcgc aagaagtgcc
     6361 cgttcagaca ggtatctttg gagcagatat gcaggttgag ctggttaata acggacctgt
     6421 taccattatc ctagatacta aaaagagata agaaagacca agcccagtcg gcttggtctt
     6481 tctcatcgat cataaaatac tccaaaaaga aatcggttct tgatatgctt gggggaagtc
     6541 tcttttcagg ctttggcaga tgcgatagga agggatgaga tgtcctagga tgaggagagt
     6601 tccctgaagg aaaagtggaa tatcacttaa agacaccaag gagagaatca tcaggctatc
     6661 ccataggaga atttgtaaag caaaacgcca gaatctcggc ctaatctggg aatagagttg
     6721 actaaagtgg tcacaaagtt ggtttgaaag ataggtcaat agcacaggat gaaagaaaat
     6781 gagccaacca ctataaatca aaatccagtc ccaagtaagc ccccctttta aatgtcaaaa
     6841 atgccagcat gattccatca atgacaagga gttgaatggt tttgatgaag atgatttttt
     6901 tcatgctaaa acctcctttt ccttagagac actcccataa agagatgtgt ttgtcgtaaa
     6961 agtctggata gttttctctt aggtagtcag ctgtcatata ataaaaagta gaatggcaag
     7021 cagtgataat tgacataaga gatgaaaaac gctgagaact aatgaaggct aagagaacaa
     7081 aaaatagaaa atcaatggtg agaaccccta aatagtagaa gcgaatcttt ttattgattt
     7141 gaggataaat ctcagcgaat tgatgtttga gtcggacgat caagagaaat agcaggagtc
     7201 cttgagcaag gaagaaaatt aaaccagaga gcaagagcca ctcaaaagat gtctcgagtc
     7261 tagtgataaa aaatgcaaat agtagcaaat caataactgc aattgctata aaatggattc
     7321 tatagagttt tttcatgaca agaccttcct cttttatcta acttcattct actccaaaag
     7381 aatgggagtt acaactaaaa tgataaaaat agcagaaggg agattctctt aagttggcta
     7441 gtattcttta tttgagtttc cttctatgta tctaacttct tcatcattcc agacaaataa
     7501 agctccgatt gcattgagga tataaaagat gtatttaccg atattggcga agtttccttg
     7561 aataccagct tttgtcagct gaacgaaatt gtaaatcaac caaaagcccc actgagttgt
     7621 tagtttcaat gcattcaaag cattggcaat gagggacagt gcaaaggcaa tagttgttac
     7681 gtaggcaagg agatccatct tgccctcata tccgatatag ttggtcacaa aggcaaagag
     7741 gaaggcgatg atggaaatga tgatggccgc caattttacc tgtttttggc tcatttggtt
     7801 gggtctgcct tcttgcgaag cttcccactt ctttatagca aaggtataaa tgaggaaggt
     7861 gacgggatag gtaatgatgg ccgccttatt tccaaggata taatcaatag caccgaacaa
     7921 aagggtatta acaataccaa agtaatttcc ccatttgctt aatttccccg tgaaacgagt
     7981 ggacaacatg gaaatcccaa cgttggttac ggaaatcaat ccaaagggta caagagctgt
     8041 ccatgatccc cagtctacaa atttatcgag gcgtgagttg agataaccag atgcaatcgc
     8101 aatcccaacg accaaagcaa ccccgaagag gtcaaatcat ttagatgtag caaaaatttt
     8161 tagtgatttt ttcataggtt aaactacctt tctttttttc aaatattctc ccattttttt
     8221 tcaaatattc taccaccaaa tgaaagtaaa ataaaatgat agaaataaaa ccctgaaaat
     8281 aaaactttca gggttggtag gggacttgag aaattagttg attttctcga catagagttg
     8341 ttttgcaatg tccatattca cttgtaaatc ctcgtcgtta ctgaggatag tgaaggtatt
     8401 gctgaagcca tcaaactgct tgacttggag ctggtcaccg atgtgaagtg agtgcttgtc
     8461 cagataatgg agaatgtcaa aattatcgtg cacccgagtc aggcggtagg cgccagcttc
     8521 cttgatatca gctagtggga ggttattgat ttcaacgagg agttctccct tggcaggaat
     8581 agttcctccg tgggggcagg ttttagggaa acctagcagt ttatctagtc tttccacgaa
     8641 caggtcagag acagtgtgtt ccaagacctc agcttcctcg tgaatctggt cacttgtata
     8701 gtctaaatga tgaactagaa aaacttcaat caagcggtgc ttacgataga gctcagagac
     8761 tagtttgaga ccgaggtcag tcagtagata gccacattcc ttgtccttta ggatgaggtt
     8821 ttcacttttc attcgtttga tcatttcagt tacggcaggg ggagagactt gcatgcgagc
     8881 cgcaatttcc ttgttggtaa tcttatgcag gtctatgcca atttcataaa tacattttag
     8941 atagtcttct ttgtttgggg tcattcgttt cctcttgtct aatatcttta tctagtatat
     9001 caaaaaaagc tagatttctc tagctgtgac tttttatgtt atacttattg caagagaaaa
     9061 aacgaggaga tggatatgac gaaaatagct cttctttcag atattcatgg aaataccacc
     9121 gccttggagg ctgttttggc agatgctcgg cagataggag tggatgaata ctggcttttg
     9181 ggagatattc tcatgccagg gacaggacgt agaaggattt tggacttgtt ggatcaacta
     9241 ccgattacgg ctagagtttt gggaaactgg gaagacagtc tttggcatgg tgtccgtaag
     9301 gaattggaca gtactcgccc cagtcaacgc tatctcttgc gccagtgcca gtatgtttta
     9361 gaggaaattt ccctagaaga aattgaagtg ctccacaatc aacctcttca aattcatcgt
     9421 cagtttgggg atttgacggt gggaattagc catcatctgc ctgataagaa ctgggggcga
     9481 gagttgattc atactggcaa acaagaggag tttgaccgct tggtgactca tcctccctgt
     9541 gatattgctg tttatggaca tattcaccag cagttgcttc gttacgggac tggtgggcaa
     9601 ttgattgtca atccgggttc gattggccaa cctttctttt tggacgctca gctacggaag
     9661 gacttgcggg cccagtatat gattttggag tttgatgaca aaggcctggt agatatggac
     9721 ttccgacggg tagactacga tgtggcagcg gaattgcagc tggctaaaga cctgagactt
     9781 ccctattttg aggtttacta tgaaagtctg gtcaatggga tccaccatac tcatcatcag
     9841 gaatttttga gagaattggc tcagaaagag ggctgcgatc gggagttaga cgactggttg
     9901 aaaagtggta acgattgaca ttacaactaa aaagggtata ataatagaaa aagaagagta
     9961 gaggcagggt agcctcgtaa aaaggagaag aggatgcaaa ttccaagtag atttaccatt
    10021 gcgactcata tgctgataat cattgccctc gaggggaagg aaagcaaggt gaccagtgat
    10081 tttctggctg ctagtgtcgg ggtcaatcct gtcattatca gaaagatctt gtcccagttg
    10141 aagaaggcag agctgatttc agtagcgcgt ggaacgggcg gaacagagat tgtcaaggac
    10201 cttaaggata ttagtctttt agatgtttat caggcggtcg aatgtcttgg taagacaggt
    10261 caactcttca gttttcatga caatccgaat ccaaattgcc ctgtaggagc tcatattcat
    10321 gatgttttgg atcaaaaatt ggagagaatt cagttgacta tggaggcaga acttggtcaa
    10381 accagtctag aaaaagtcgt ggccgatgca gagagtcaga tgaaggatta agagggataa
    10441 atgccctctt ttttctttgg ataattatga taaaatgtag ctattaaagg agtaagaaat
    10501 gtatctcgtt ataggaattg tattagcctt tatagtgtca ttttggaagg ataatagaag
    10561 tttgtggaat ccagtattat ttttattatc cctcatttca agttattttt atctatccta
    10621 ccttttttat aaaaatggat acgagaatgt tcagttagct ttttatgtat ttgcctttgt
    10681 cttacttcct tttttgcttt ttctaagtgg catcttttta atctataatg gggtcatttt
    10741 gttgaagcga gagggacgtt ccaagcccca ttatctctca atgctctttg atttttattg
    10801 actattagat tctatttaaa aagatttaga atgcacatga aagtatgtat tctaggtctt
    10861 ttgttttgta tacatttaat tttcaagagt attatctatt tttccatgcc tggtaacgag
    10921 tatcaatcaa taactgggta aggcgaccca aggtttctct ttcttctgga gtgagggatt
    10981 gagcttggac aaagagatga tgaatgtatt caagcgcctt taaggaagcc aagtcattga
    11041 tagagctaat accagcctca agaatagtgc caaagaagag gtcgaagtag agctgaagtt
    11101 cttcgtcagg gactgtggcc acccattctt taaaggttgt gtctacttgc tggctatcac
    11161 tgttggtctt atccagttgg acgaagtgct tgtcctcaat ctgccaacta aaggtatcgt
    11221 gctgggcgat gccacccagg gcagtactct gaacgatgat ttggtgagca ggaatttcca
    11281 gcatcatacc gataatggaa ccttgtggaa tgaatatctt gcttctatcc attatccttt
    11341 gataacccgc agtctgtgtc aattcttgat ggagaccagg tgcatcaaat gtataaactg
    11401 ctgtgatctg attttgcaaa ctttgctcaa tttggctagc agcatagata gcgagatttc
    11461 ctcccttgga atgcccagcc agaataactt tttgcttagg atgatgggca aaaaagttct
    11521 ttaaatagcg aagggcgtgc ttttgagcag gaatttcctt catataggtc aggtggaaat
    11581 cttccttcca gccaatgata ctgtcatctg tcccacgaaa gacaatcaga taggtatcga
    11641 ggctgacacg ataagtcata gccgcaaatt gcttttgcag ttcagggtcg atgtcgttga
    11701 taaaatggga gagtttgcaa tttttgaagc gcttgtgttg agccaattta tctaataatt
    11761 gaaggcgatt tttgctagta agcatggtgg gctctcttgg aacctgaggt gctagatcta
    11821 aaagacgttg aggaagtgtg gagaccagat tatcaaagga gaggtaggtg atttctatta
    11881 aggttagaat gtctaactca ttcaagggaa ggtcgtaata agaatcatat gcgacatctt
    11941 tcagatagtc aaaaatattg gccataagag ctcctttctt tttatttatc ttagcaaatt
    12001 tccctcaaat tagctagtag cattgcctgt ttgtactggc taaaaacagg ctatttcaaa
    12061 ttcagtttca gaccatctgg catggaaaaa tctgttataa taatggaaaa ggagaagcgc
    12121 atgcacaaga ttttattagt ggaagatgat caggtcattc gtcaacaggt tgggaaaatg
    12181 ctctctgaat ggggatttga agtggtcctg gtagaagact ttatggaagt tttgagtcta
    12241 tttgttcagt cggaacctca tctggtcctc atggatattg gtttgccctt gtttaatggt
    12301 tatcactggt gtcaggaaat ccgcaagatt tccaaggtac ctatcatgtt tctttcttcg
    12361 agagaccagg ctatggatat tgtcatggca atcaatatgg gggcggatga ctttgtgacc
    12421 aagccttttg accagcaggt tcttttagct aaggttcagg gcttgttgcg tcgttcctat
    12481 gagtttgggc gtgatgagag tttgctggaa tatgctggtg ttatcctcaa taccaaatcc
    12541 atggatttac attatcaagg gcaagtcttg aatttgacca agaacgaatt ccagatttta
    12601 cgcgtgttat ttgagcatgc aggcaacatc gtagcacgtg acgacctgat gcgggaactt
    12661 tggaacagtg actttttcat tgatgataat accctctctg tcaatgtggc tcgtttgcgt
    12721 aaaaagttgg aggagcaggg attggtagga tttatcgaga ccaagaaagg aatagggtac
    12781 ggattgaagc atgcttgatt ggaaacaatt ttttctagcc tatctacgct cccgtagtcg
    12841 tctttttatc tatctgcttt ctttggcatt tcttgtctta ctctttcagt ttttatttgc
    12901 cagtctagga atttacttcc tctacttttt cttcttgtgt tgctttgtaa ccatcttatt
    12961 tttcacttgg gacatattgg tggaaacgca ggtctatcgc caggaacttc tctatggaga
    13021 gagggaagcc aagtctcctt tggaaatagc tttagcagaa aaattagaag cgcgtgagat
    13081 ggaactctat cagcagaggt caaaagcaga aagaaaactg acggatttgc tggattacta
    13141 taccttgtgg gtccatcaga taaagacccc cattgcagcc agtcaactct tagttgcaga
    13201 agtggtcgac cgccaactga agcagcagct agaacaggaa attttcaaaa tcgactccta
    13261 taccaaccta gttttacagt acctgcgttt agaaagtttc catgatgatt tgctcttaaa
    13321 gcaggttcaa attgaggact tggtcaagga aataattcgt aaatatgctc ttttctttat
    13381 tcaaaaaggc ttaaatgtca atctacatga ccttgataaa gaaatcgtga cggataaaaa
    13441 gtggctgcta gtggttattg agcaaatcat ctcaaacagt ctcaagtaca ccaaggaagg
    13501 tggtctggag atttatatgg atgaccaaga gctttgtatc aaagatacgg gaatcgggat
    13561 aaaaaacagt gatgtcctcc gagtatttga acgtggcttt tcaggataca atggccgttt
    13621 gacccagcag tcctctggac ttggccttta tctatctaag aaaatttctg aagaactggg
    13681 gcaccagatt cgtatcgagt ctgaggtcgg aaaaggaacg acagtgcgga ttcagtttgc
    13741 tcaagtgaac ttagtccttg agtaaaaata gatgagaaaa agcctccaga ttggaggctt
    13801 ttatgctaga ctcttatttc tcaacgcgtg atttgttggc gatatcagct aggatagctt
    13861 gaacaaaatt atcaactgag acagtttgtg tttctttttg gccgtagcga cgaacgttga
    13921 ctgtttcgtc ttccatttct ttgtctccaa caattaattg gtaaggaatc ttgctggttt
    13981 gtgaagcacg gatcttgaac tgcatttttt cattgcgctc atctacgtct gcacggacac
    14041 cgcggtcacg gagtttcttg gccacttccc aagcgtagtc cacgtgtttt tcgttagata
    14101 ctgggatgag ggttacttgg tgtggtgcca gccatgttgg gaaggccccc ttgtagttct
    14161 caatcaagat agctgtgaag cgttccatag ttgagataac cccacggtgg atcatgactg
    14221 gacggtgatc ttcgccatca gctccgatgt atttgaggtc gaagcgttct ggcaacaaga
    14281 aatcaagttg gatagtagaa agggtttctt cttttccaag ggcagtttta atctggatat
    14341 ccaattttgg tccgtagaag gctgcttcac cttcggcttc aaagtagtcc acgcccattt
    14401 catcaagagc tgcacgaagc atggtttggg cattttccca catctcatcg ttatcaaagt
    14461 acttatgagt atcttgaggg tcacgaagag agaggcggaa gcggtagtca gtcaagttga
    14521 agtcttcata aacatcgata atcaactgaa gggcacgttg gaattcttct tggatttgtt
    14581 ctggagtaac gaataggtga ccgtcgttga gtgacatttc acgtacacgt tgaaggccag
    14641 tgagggcacc agatttttcg taacggtgca tcataccgat ttcagcgata cggattggca
    14701 attcacggta agagtgaaca tggtgtttga aaacttggat gtggtgcgga cagttcattg
    14761 gacgaaggac aaattcttcc ccgtcaccca tgtccatggt tgggaacatg tcttcttggt
    14821 aatgatccca gtgaccagaa gtcttgtaaa gctcaacaga agcaagtggt ggagtgtaga
    14881 cgtgttggta gccagaagcc aactctttgt ttacgatgta gcgttccaat tcacgacgga
    14941 tagtcgcacc atttggcaac cagaatggca aaccttgtcc cacttcttgt gaaatcataa
    15001 agaggtcaag ctctttacca agtttacggt ggtcacgttc cttagcttct tcacgcattt
    15061 gaaggtagtt tttcaagtct ttcttgtcaa accaagctgt accgtagata cgttgcatca
    15121 tagcattgtc gctgtttcca cgccagtacg caccagctac atggagaagg tggaagattt
    15181 ggatacgacc tgttgatgga acgtgaggtc cacggcagag gtctacatat tcaccctgac
    15241 gatagatagt caaaccgcct tcgtcttctg agtgttcttc aatcaattcc aacttgtaag
    15301 ggtcattttt gaagatttca cgtgcctcgt ctttagtcac ttcttcacga atagatggga
    15361 agttttcttt gacgattttt tgcatttctt cttcgatacg aggaaggtct tcgttagaga
    15421 tttgaccagc tgtgttgtca gtatcgtagt agaaaccatc ttcgatggct ggaccaactc
    15481 ccaagtgaat gtctgggaaa agacgacgag ctgcttgggc gaacaagtga gctgctgagt
    15541 gacgcaagat tggaagggca tcttcgtgat caggtgtcac aatttcgatg cttccatctt
    15601 cagtgatagc gcgagtagtg tcgatgagtt tgccgttgaa tttaccagcc aaggcttttt
    15661 tagctaggga attgctgata gattgggcaa tttcaaaagt tgttacgcca gattcgaatt
    15721 cacgaacagc gccatctggg aaagtaatgt taatcatgat gttctccttt tttattattt
    15781 catagtttct aagcattgat tttttatcag cagactactt ccaagtccta acgttcaatt
    15841 gcttttcttt atcagagagg aagttttcca tttccgtcag aatgtctata tccaaacgtt
    15901 gagaaagttc taatagccac cagatatttt ctgaaagttt ttgttccagt gtgtagggtg
    15961 tttcatcata gtagcgtcct tgctttgtca tcaccagtcg ttggaaattt ccaatatcat
    16021 tagataaagc caagaggtct tcttctaccg tccacttgaa atcatgatgc ttaacttcca
    16081 gttcgtgata agcttgtcgg attgcccaac tgcgctccac taattcttgc aactccataa
    16141 ttagtatcct aataaaaaaa tgcgacatcc tcgaaaggac gtcgcaacgt ggttccacct
    16201 tcatttatgt acctcaaaag agagggacac ctctgatcgg ctctaacgtg gccaccgttt
    16261 tatgttttca taaaaaactc aagagtagta tcagtttaga cttcctatgc gtttccagca
    16321 accacgcact ctctagtaga aagggactaa gtgacttgtc tctatggatt attatagcat
    16381 gattgtgaga tttttcaagg attttgtgaa agttttttgt ttttcatttt ttgtagaata
    16441 tgcttgatgc taataccaga atccatgccg accagagcaa ctgtttcata gattagcaag
    16501 taaattagta taaactcacc aaaggttatg atagtaaagt aaaagagcag tccgttacca
    16561 actaaccatc aaagtgagaa acggaaacgg tagctataga gtaacgatat aatcaagatt
    16621 accagaggtg taaagaagag aagattgttt atatagagtt ccttgagtgt aagggggtca
    16681 gaaacaacta gtaagaacaa ctcataaagt ggccaataaa agaataatat gaccagatag
    16741 taggggagat gggaatagaa tctacgcatg ataatcacct cgttttaaaa aaacagacaa
    16801 cttagatctc ttgctatgat tattatagca tgtttaaaac aagaaagtaa atttgttgac
    16861 aaagaagatt tttcttttca ctagaatcat agttagtttt cttggtagaa atattttgat
    16921 aataattaag caagactatt gacattgatt attaagagtg tcacaattat tgtaactaaa
    16981 ataattacaa caagagaggt ataaaaaatg aaactagcag ttattgcagc aaatggacaa
    17041 gcggggaaag caattgttga agaagcagtc aaacgaggac atgaagtaac ggctattgtt
    17101 cgttctgaaa acaaaagtca agctgagtct attattaaaa aagatttatt tgagttgaca
    17161 aaagatgatc ttactggatt tgatgctgtt atttcagcgt ttggtgctta tacaccagat
    17221 acgctaccgc tccacagcaa atcaatcgaa ctatttaatc agcttcttgc tggaacacag
    17281 acacgttttc tagttgtagg gggagcagga agtctttata tagatgaaac aaaaacgaca
    17341 cgacttttag atacgtctga ttttcctgaa gaatttaagt cactagcaaa agatcaagcg
    17401 gatgaattgg atcttcttcg tacaaaaaat aatctcaact ggacttttgt cagtcctgct
    17461 gtagatttta ttcccgatgg tgaaaaaaca ggaaactaca ttctagcagg agaaattttt
    17521 actactaatg aaaaaggaat aagccagatt agttatgcag attatgctat tggattggta
    17581 gatgaacttg aaaaaggtca tcatataaaa gaacgtatct ctttacttga aaaataataa
    17641 ctatgagagt attaatgttt gacagaaaat ttaaatcttg gtaaaatagg gattgtcgta
    17701 aagacaaata acttcttctt ggttacaggc atgccaacct gtcactcgga tgaagccaaa
    17761 taaaaaggag aaacatcatg gcaatctcaa aagagaaaaa aaatgaaatc attgcacaat
    17821 atgcacgtca cgaaggtgat acaggttcag tagaggttca agttgctgtc cttacttggg
    17881 aaatcaacca ccttaacgaa cacatcaaac aacacaaaaa agaccacgct acttaccgtg
    17941 gattgatgaa aaaaatcggt cgccgtcgta acttgcttgc atacttgcgt aaaaacgacg
    18001 ttaaccgtta ccgtgagttg atcaactctc taggacttcg tcgctaattc aagatacaag
    18061 tgcttaggcc gtgttcaatt gagcatatct tgataatgaa gctactctaa attgggtagc
    18121 ttttttgcgt tgtcacctta cctcgatata ctcaagtata atctttggtt acggttccta
    18181 gcactgtaag gtaaaataaa ccagcttttc tcccttcggg gagatttttt tgtttcactg
    18241 aaagtttcgt acgatcttcg cctcgtcgcc tagtctataa acgatttatc tagcaagaat
    18301 catgatgcta agggcgtcaa aaatccgtat gaaaataggg aaatgacaca gtgttcgatg
    18361 agcacaagga gtttcatctt tttcactagg attttagccc gggctcaaat cagctctctg
    18421 acttcagagg gcttttttat tgaggtttta tcggtaacaa ttttggagac tacaaaaacc
    18481 tcaaacggta tcagctaatt acaccataaa ggtgcgcagt tactcggctt ttcaagccga
    18541 gtagctgtat gcaagccccc tagaagagcc cactctttac aaagtaaagt atagtatgtt
    18601 atactttaca tggaagtagt cactgaattc cagttagaaa ttactttgta actacgtttt
    18661 gaggaggagt aaaatgcttt cctacgttcg acattaccca ctagcgatag ctaaattaat
    18721 gtgtctgtgc tctcctaaaa tctgctgatt tattactgac taatacagga gtttttttta
    18781 tgggacagac aatcatatct gctattggtg tttatatttc caccagtatc gattatttaa
    18841 ttattttaat tattttattt gcacagctat cacagaataa acagaaatgg catatttatg
    18901 cggggcaata tctaggcaca ggcttacttg taggggcgag tttagttgct gcttatgtcg
    18961 ttaatttcgt gcctgaagaa tggatggttg gattgcttgg tttaatccct atctatttag
    19021 ggattcgctt tgcaattgtt ggagaagatg cggaagaaga agaggaagaa attattgaaa
    19081 gattagaaca aagcaaggca aatcaactgt tttggacagt tacattgctg acaattgcgt
    19141 ctggcggaga taatttaggt atctatatac cttattttgc ttcgttagat tggtcacaga
    19201 ccctcgtggc cttgcttgtg tttgtaatcg gcataattat cttttgcgag attagtcggg
    19261 tgttatcctc tattccgtta atattcgaga caattgaaaa atacgagcga atcattgtgc
    19321 ccttagtatt cattctactt ggactataca tcatgtatga aaatggtacg atagagactt
    19381 ttctgatcgt gtagattttt ttgtttcact aggattttag cccgagctca aatcagctct
    19441 ctgattttca gagggctttt tcattgagta taaatggaca atcttaaagt tactctattg
    19501 tagagtggct tttgatttga ttaggagatt cttataaata tagtaaaatg aaacaaaaac
    19561 aggacaaatc gatcaggaca gtcaaatcga tttctaaaaa tgttttaaga agtagaggtg
    19621 tactattcta gtttcaattt gatatataaa tgaaaacctt agctgtttgg ccaaggttca
    19681 tttcttctat gcaagttctt ctcttttctt atctgggttc cagatgaagc ttgcgataaa
    19741 gctaaagatg atggttagga tagcgaggat aatagcaagg atgagggcag ggatgcggat
    19801 aaaccaccag agtggatttg gttttttatc attgtgccat tgtttcgttt cttcgtccag
    19861 acgttggaat tctgtttgaa tacgattggc gactgtttca atgccttcat cattcatttt
    19921 cttgatatct gagatatcga ttggatttcc aaagttcata tcgacacgtt cacggctaat
    19981 caagcccttc aaagtcatgg gaccggtgta ggtaaccggc atgatacgga ccttggccat
    20041 tttggcaatc agtgctgcgc cccccttgac atcgtttgag tggcggctac cacttggaaa
    20101 catgatgaga gagcggtcac tttttttgag aacgttgata ggatatttga tggctgaggc
    20161 gctgggattt tcacggtcga tgggaaaggc gccacacata cgaatccacc aaccaaagat
    20221 acggttggta aagagttctt tttttgccat aaagatgaac tgttttggct tggtcgcaaa
    20281 ggccatataa acaggatccc accaggtacg gtgaggcgca actaaaatat aattttcatc
    20341 ttgattagga attttatcag tattatgata gtgagcattg ccattgatgg accataagag
    20401 caatacaact aatccacgca aataagtata aaacatgcga tctccttcga ttgttttctt
    20461 gttattatta taccttatca aaggagggct ggcaaacttt tcccttgact agatgcatat
    20521 ttgggatgaa attagaattc tgttaaaaaa aatgatataa tagaatttat ggataaaaat
    20581 aagattatgg gattaaccca aagagaagtc aaggaaagac aggctgaggg tttggtcaat
    20641 gactttaccg catcagccag taccagcact tggcaaatcg ttaaacgaaa tgtctttacc
    20701 ctttttaacg ctttgaactt agccattgct ttggctcttg cctttgtgca ggcttggagc
    20761 aatctggtct tctttgctgt tatctgcttt aacgcttttt ctgggattgt gaccgagcta
    20821 cgagccaaac acatggtgga caagctcaat ctcatgacca aggaaaaggt caaaaccatc
    20881 cgtgatggtc aggaagttgc tcttaatcct gaagaattag tgctaggaga tgtcattcgt
    20941 ttgtctgcag gagagcagat tcctagtgat gccttggttt tggaaggctt tgcggaagtc
    21001 aatgaagcca tgttaacggg agaaagtgat ttggtgcaaa aggaagttga cggcttactt
    21061 ttgtcaggaa gtttcctagc cagtgggtca gttttatctc aagttcacca tgtcggtgca
    21121 gacaactatg ctgccaaact catgcttgag gctaagaccg ttaaacccat caactcccgt
    21181 atcatgaaat cgctggacaa gttggctggt tttactggga agattatcat tccctttggt
    21241 ctggctctct tgctggaagc cttgctttta aaaggcctgc ctctcaagtc atctgttgta
    21301 aactcgtcga cagctctttt gggaatgttg cctaagggaa ttgccctttt gaccattact
    21361 tcgctcttga ctgcagttat caagctaggt ttgaaaaagg tcttggtgca ggagatgtac
    21421 tctgttgaga ccttggcgcg cgtggatatg ctctgtctgg acaagacggg taccatcacc
    21481 caaggaaaga tgcaggtgga ggctgttctt ccgttgacgg aaacgtatgg tgaagaggct
    21541 attgccagca tcttgactag ctacatggcc catagtgagg ataagaatcc aactgcccaa
    21601 gccattcgcc agcgttttgt gggagatgtt gcttatccta tgatttccaa tcttcccttc
    21661 tccagcgacc gcaagtgggg ggctatggag ttagaaggct tggggacagt tttcttaggg
    21721 gcacctgaga tgttgctgga ttctgaagtc ccagaagcca gggaggcctt ggagagagga
    21781 tcacgtgtct tggtcttagc tctcagtcag gagaaattag accatcacaa accacagaaa
    21841 ccatctgata ttcaggctct agccttgctg gaaatcttgg accccattcg agagggagca
    21901 gcagagacgc tggactatct ccgttctcag gaggtaggac tcaagattat ctctggtgac
    21961 aatccagtta cggtgtctag cattgcccag aaggctggtt ttgcggacta tcacagctat
    22021 gtagattgct caaaaatcac cgatgaggaa ttgatggcca tggcggagga gacagctatt
    22081 ttcggacgtg tttcccctca tcaaaagaaa ctcatcatcc aaacgttgaa aaaagcgggt
    22141 catacaacgg ctatgacagg ggacggggtt aatgatatct tggcccttcg tgaggcggat
    22201 tgttctatcg tgatggcgga gggggatcca gcaacccgtc agattgccaa tctggttctc
    22261 ttgaactcag actttaatga tgttcctgag attctcttcg agggtcgtcg cgtggtcaat
    22321 aacattgccc acatcgcccc gattttcttg ataaagacca tctattcctt cctgttagca
    22381 gtcatctgta ttgccagtgc tttactaggt cggtcagagt ggattttgat tttccccttc
    22441 attccgatcc agattaccat gattgaccag tttgtggaag gtttcccacc attcgttctg
    22501 acttttgagc gaaatatcaa acctgttgag ccaaatttcc tcagaaaatc catgcttcgt
    22561 gccctaccaa gcgctctcat ggtcgtgttt agcgtcctgt ttgtgaaaat atttgggagt
    22621 agccaaggtt ggtctgagtt agaaatctca actctcctct attatctctt ggggtcaatt
    22681 ggtttcttat ccgtatttag agcctgcatg ccattcactc tatggcgagt cctattgatt
    22741 gtctggtcag taggaggctt cctagccaca gctctcttcc caagaattca aaaactgctt
    22801 gaaattgcaa ccttaacagg acaaacatta cctgtttatg gtgtcatgat gttggtcttt
    22861 accgtgattt tcatcctgac cagtcgttac caagagaaaa aataaagaaa gactgcaatc
    22921 tgtggattgc agttttttta ggtacaagat tgccagccga aatgtggtat aataagaggt
    22981 aatagagttt tggaaagtga gagaagatga tttcaaagag attagaattg gtagcttcct
    23041 ttgtgtcaca gggggctatt ttactagatg tgggaagtga ccatgcttat ctgcctatcg
    23101 agttggttga gagaggccaa atcaaaagcg ctattgcagg tgaggtggtg gaaggtccct
    23161 atcagtctgc ggttaaaaat gttgaggctc acggcctaaa ggagaaaatc caagtccgtt
    23221 tagccaatgg cttggcagct tttgaagaga ttgaccaagt gtctgtcatt accattgctg
    23281 gcatgggtgg ttgtttgatt gctaggattt tagaagaagg tttggggaag ttagctaatg
    23341 tagagcgttt gatcctccag cccaataatc gtgaagacga cttgcgtatc tggctacaga
    23401 atcatggatt ccagattgta gcagaaagca tcttagaaga agctggcaag ttttacgaga
    23461 ttttggtggt ggaagcagga caaatgaagc tatcagccag tgatgttcgc tttggtccct
    23521 tcttgtccaa agaagtcagt ccagtatttg tccaaaaatg gcaaaaagaa gctgagaagc
    23581 tagagttcgc cctcggacaa atcccagaaa aaaatctgga agaacgtcaa gttctagtag
    23641 ataagattca agctatcaag gaggtgcccc atgttagcaa gtgaagtgat ccaagcttat
    23701 gaagcctttt gccctcagga attttctatg gagggagaca gctgtggtct gcaaattggc
    23761 actttagaca agggtatcca aagggtcatg gttgccctcg atattcgtga agagacggtg
    23821 gctgaagcca ttgaaaaggg tgtagacttg attatcgtca agcacgcgcc tatctttcgt
    23881 cctatcaagg acttgcttgc cagccgtccg caaaatcaga tttacatcga cctgattaag
    23941 catgacatcg cagtttatgt cagccatacc aatattgata tcgttgaaaa tgggctcaat
    24001 gactggtttt gtcagatgct aggaatcgag gagacgactt atctgcagga aacaggtcca
    24061 gaacgtggaa ttggacgtat tgggaatatt cagcctcaga cattttggga attggcccaa
    24121 caggtcaagc aagtctttga cctagatagc cttcgaatgg tgcattatca agagaatgat
    24181 ttgcagaagc ctatttcaag agtagcaatt tgtggtggaa gcgggcagtc tttctataag
    24241 gatgctttgg caaagggggc agatgtctat atcactggtg atatctacta ccatactgct
    24301 caggatatgc tgtctgatgg cttgttagca ttggatccag gtcactatat cgaagtgctt
    24361 tttgtggaaa aaatcgcagc actccttact caatggaaag agaaaaaagg ctgggaacta
    24421 gggattttac ctagtcaagc atcgaccaat cctttccgtc atatctagtt agaaagtgaa
    24481 gacaatgaaa aaagttgcca ttattggagc aggaattgtg ggagcaacag ctgcctacta
    24541 cctctcgaga gaaagtgacc tagaggtgac cgtttttgac catgagcaag gtcaagccac
    24601 caaggccgca gcaggaatta tcagtccttg gttttccaaa cgccgtaata aagcctggta
    24661 caagatggcg cgcttggggg ctgattttta tgtggattta ttagctgatt tagagaaatc
    24721 aggacaaaaa atcgactttt accagcgttc gggagtcttt ctcttgaaaa aggatgaatc
    24781 caagttggaa gaactttatc aactggccct ccagcgcaga gaagaatctc ccttgatagg
    24841 gcaattagct attctggacc aagcctcagc taatgaatta ttccctggtt tgcagggatt
    24901 tgaccgcctg ctctatgctt ctggtggagc gagagtagat ggccacctct tagtgactcg
    24961 tttgctggaa gccagtcagg tcaagctggt caaagaaaaa gtgactctga caccgttagc
    25021 atcaggctac cagattggtg aagaggagtt tgagcaggtt attttggcga cgggagcttg
    25081 gttgggggac atgttagaac ccttgggtta tgaagtagat gttcgtcctc aaaaaggaca
    25141 actacgagat tatcagctgg tccaagacat ggaagcttac cctgttgtca tgccagaagg
    25201 ggagtgggat ttgattcctt ttgcaggtgg gaaattgtcc ttaggcgcta cccacgaaaa
    25261 tgacatggga tttgatttga cggtagatga aaccttgctc caacaaatgg aggaggccac
    25321 cttgactcac tatctgattt tggctgaagc tacttcaaaa tctgagcgtg tgggaatccg
    25381 tgcctatacc agtgatttct ctcccttctt tgggcaggtg ccagaattgg caggtgtcta
    25441 tgcagccagt ggactaggtt catcaggcct cacaactggt cctatcattg gttaccatct
    25501 agcccaactg atccaagaca aggagttgac cttggaccct ctaaattacc caattgaaaa
    25561 ctatgtcaaa cgagtaaaaa gcgaataata ctcaatgaaa atcaaagaac aaactaggaa
    25621 actagccgcg ggttactcaa agcactgctt tgaggttgtg tatagaactg acgaagttag
    25681 ctcaaaacac tgttttgagg ttgtgtatag aactgacgaa gtcagtaacc atacccatgg
    25741 caaggcgacg ttgacgtggt ttgaattgga ttttcgaaga gtataagcat tttactgaaa
    25801 ttttatcagg tagtttagga tggcaaatga cattccctgt caaaaatggt aaaataagaa
    25861 aaaataatct gagaatcgag gaaaaaagat gcaagaaaag attttggtaa ctggtggtgc
    25921 cggttttatt ggaacccaca ctgttattga attgatccaa gcaggccatc aagttgttgt
    25981 ggtggataat cttgtcaata gcaatcgtaa gagtttagaa gttgttgaaa gaatcacagg
    26041 agttgagatt cctttctatg aggcagatat tcgtgacaca gataccctca gagatatttt
    26101 caagcaggaa gaaccgactg gtgtcattca ctttgctggt ttgaaggctg ttggcgaatc
    26161 aacacgtatc cctcttgcct actatgacaa caatatcgct ggaactgtca gccttttgaa
    26221 agccatggaa gaaaacaact gtaaaaacat catcttcagt tcttctgcga cagtttacgg
    26281 ggatccgcac acagtgccca tcttggaaga tttcccactt tcagtgacca acccatacgg
    26341 tcgtaccaag ctcatgctag aggaaatttt gaccgatatt tacaaagcag actcagaatg
    26401 gaatgttgtc ttgcttcgtt actttaaccc aatcggagcc catgagagtg gtgatttggg
    26461 agaaaatcca aacggtattc caaacaatct cttgccatat gtgactcaag tagccgttgg
    26521 aaaattagag caagtgcaag tgtttggaga cgattacgat acggaagatg gaacaggtgt
    26581 tcgtgactat atccacgttg tcgatttggc taagggtcac gttgcagctt tgaaaaaaat
    26641 ccaaaaaggt tcaggactaa acgtttataa ccttggaact ggtaaaggtt actcagttct
    26701 tgaaattatc caaaacatgg aaaaagcggt gggacgtcct attccttacc gcatcgtaga
    26761 acgtcgccca ggtgatatcg ctgcctgcta ctcagaccca gcaaaagcta aagcagaact
    26821 cggttgggaa gcagaactcg acatcaccca aatgtgtgaa gacgcatggc gttggcaaag
    26881 caagcatcca aatggatttg aagactaaga tgatgatttc aatcatcgtc ccttgtttaa
    26941 acgaagagga agtacttcct cttttttatc aggctctgga agctttactt ccagatttgg
    27001 aaacagaaat cgagtatgtc tttgtcgatg atggatcaag tgatgggacc ttggaactct
    27061 taaaggccta tcgggagcaa aatccggcag tccattatat ttctttctct cgaaattttg
    27121 gcaaagaagc agccctttat gcaggcttgc aatatgcgac aggagatttg gtggtggtga
    27181 tggatgcaga cctccaagat cctcctagta tgttgtttga gatgaaaaat gtactagaca
    27241 aaaatgtaga cttggactgc gttgggacac ggagaactag tcgggaggga gaacccttct
    27301 ttcgcagttt ctctgctgtt ctcttttatc gcctcatgca aaaaatcagc ccagtagctc
    27361 tgccgtcggg tgtccgtgat tttcgtatga tgagaaggtc tgtagtcgat gccattttaa
    27421 gcttgaccga gtccaatcgt ttttcaaagg ggctcttcgc ctgggtgggt ttcaaaacct
    27481 actatttgga ctatccaaat gtcgaaaggc aggctggcaa gacaagttgg agttttaggc
    27541 aactcttttt ctactccatt gaagggatta ttaatttttc agattttccc ttgattatag
    27601 cctttgtagc tggtctccta tcttgttttc tttctctgct gatgaccttt tttgttgtgg
    27661 ttcggaccct cattttgggc aatccgacat ctggttggac ctctctgatg gctgttattc
    27721 tctttcttgg aggcattcaa ctcttgacca ttgggattct cggcaagtat atcagtaaga
    27781 tttatttaga gactaaaaaa agaccacttt atcttatcaa agaaaaaagt gaccttcctg
    27841 attttacaga aaaaaataaa gtgaaaagac tataatttta catggaaatg tgctaaacta
    27901 gagggagtgg attatcgcca ttgatttagg cttcctggtt tccaatcagg gcgcgagttg
    27961 ggcaattttt aacagcctct aagacatcct gactaggaga aatttctttt tccagttggt
    28021 cagggtcatc gtaaaaacgc acgattccat tatcgtggta atcaaataaa tcagaataag
    28081 tttggcaaag cccacaggcg atacatcgtt caggtataag tgtgattttc atgttcatat
    28141 ttatattgta ataagaaaag taaaaaaaaa caaggagtaa ggtatggcaa aagaaccgtg
    28201 gcaagaagat atctatgatc aagaagaatc aagagcagag cgtcggcatc gaaaccacgg
    28261 aggggcggat aggatggcta atcgtatttt gacgatccta gctagtattt tctttgtaat
    28321 tgtggtggtg atggtcatcg ttctcatcta tctatcatcg ggggggagta atcgcacagc
    28381 agccttaaaa gactttcatg attctgatgc aagtgtagta caaatctcat cttcaagcag
    28441 ttctcagcct gagcagagtt cagagccaga atctacttct agtagttcag aagaagctgc
    28501 taatcctgaa ggaacgatta aagttctcgc aggagaaggg gaagcagcta ttgccgctcg
    28561 tgcaggaatc tccattgctc agttagaggc cttgaatcct gggcacatgg ctacaggatc
    28621 ttggtttgct aatccaggtg atgttataaa aataaaatag gagccgatat gaaaacaatt
    28681 caaattgcta ttgatggtcc tgcttccagc ggtaagagta cggtcgcaaa gattattgct
    28741 aaggattttg gattcaccta ccttgataca ggagctatgt atcgtgcagc gacctatatg
    28801 gctcttaaga accaattagg agttgaagaa gtcgaagccc ttctagcctt gttggaccag
    28861 catccaatca gctttggacg ttcagaaact ggagaccagc ttgtttttgt aggagatgtg
    28921 gatattaccc atcctatccg tgaaaatgaa gtgaccaatc atgtttctgc tattgcagca
    28981 attcctgaag tgcgtgagaa actggtttct ctccaacaag aaattgccca gcaaggcggg
    29041 attgttatgg atggtcgcga tattggaact gttgtattgc cgcaagcaga attgaaaatt
    29101 ttcctagtag cttctgttga tgagagagca gagcgtcgtt acaaggaaaa tattgccaag
    29161 ggaattgaaa cagaccttga aaccctaaaa aaggaaattg ctgcgcgtga ctacaaggat
    29221 agtcatcgtg agacttctcc tctcaaacaa gcagaggatg ctgtctacct tgatacaact
    29281 ggtttgaaca ttcaagaagt agttgaaaaa atcaaagcag aagctgaaaa aagaatgtaa
    29341 atgtagaaaa gccgatagat tgatatcggt tttttctaaa tttgtcaaaa tgctgatttt
    29401 agggtataat agttgctgtt cgagaaattt tgattttcaa agaatagtga atgatgataa
    29461 ggagaaatca tgaacaattt accaaattgt ccaaaatgta actcagagta tgtctacgaa
    29521 gacggtgccc tactggtttg cccagagtgt gctcatgagt ggaatcctgc tgaagttgca
    29581 gaagtagaag aaggtcttgt cgctatcgat gccaacggaa ataaattggc tgatggtgat
    29641 acagtaactc tcatcaagga cttgaaagta aaaggtgcgc caaaagattt gaaacaaggg
    29701 acgcgcgtga aaaatatccg catcgtagaa ggcgaccaca atatcgactg taagattgat
    29761 ggttttggtg ccatgaaact taaatcagag tttgtgagga agatttaagc atgtcaaaac
    29821 actataaact tgtattttat agccgtatct tcttgtttct agcggctttt acgggagttt
    29881 atcttgaaat tactaggcat ggtggttttg ggatgcttct ctattacacg gttctgtcca
    29941 acctcttggt aactattttt accctttatc ttctaaaggt tatgagccgt gtaggtgaaa
    30001 attggcaaag gccaagtctc ttgcgcttaa aaggtggggt caccatgagt atcatgatta
    30061 cctgtgtgat ttaccatttc ctcttggcgc ccattgcgac taatttctat accctagaaa
    30121 atttcctttg ccactatatc gttcccatct ggtttttagc ggataccctc ttttttgaca
    30181 aacagggtca atacaagatt tgggatccag cagtgtggac cattttaccc tttctgtata
    30241 tgatgtttgc tctttttaat ggcttggttc taaaactcaa tattccaaat gccaaggata
    30301 atcctttccc ttacttcttt ttgaatgtga acaagggttg gaatgttgtg tttaagtggt
    30361 gtctgattat ctttgttgcc tatatggtag caggatttat tttctacttt atcaagcaaa
    30421 tcaagagaaa gtcatcctaa tactcttcga aaatctcttc aaaccacgtc agtttccatc
    30481 tgcaacctca aaacactgtt ttgaacaacc tgcggctagc ttcctagttt gctctttgat
    30541 tttcattgag tataagatac aagggattca ttttcgaatt taagaaggag tctacaaacc
    30601 aatgaggctt cttcttttct tgcttatttg ataactatca aaaaagtaga agattaagag
    30661 aaatatagaa agagatttca tgaaacaatt tttagaacgg gccagcattt tggctctctc
    30721 cctcgttttg attacctcct tttccatttc gagtgcccta ccagccatgt ttgactatta
    30781 tcagggttat tctaaggaac aaattgagct cttggtgagc ttgccttcct ttggaatcat
    30841 gatgatgtta ctgctaaatg gtttcttaga aaaaatattt cctgagcgct tacagattag
    30901 tttgggcttg ctgattttat cattgagcgg tacagctccc ttctggtacc aagcctatcc
    30961 ctttgtcttt ggaacacggc ttctctttgg tttgggtctt gggatgatca atgccaaggc
    31021 catttctatt atcagtgaac gctaccaagg aaaaaggcga attcagatgt tagggctacg
    31081 cgcttctgca gaggtcgttg gagcttctct cattaccttg gccgtcggtc agttgttggc
    31141 ctttggttgg acagctatct ttctagccta tagtgctgga tttttggtgc tgccccttta
    31201 tctgctcttt gtcccttatg gaaaatcaaa gaaagaagtc aagaaaagag cgaaggaagc
    31261 aagtcgttta actcgagaaa tgaaaggttt gatttttacc ttagctatcg aagcggcagt
    31321 tgtagtttgt accaatacag ctattaccat ccgtattcca agtttgatgg tggaaagagg
    31381 attgggggat gcccagttat ctagttttgt tcttagtatc atgcagttga tcgggattgt
    31441 ggctggggtg agtttttctt tcttgatttc tatctttaaa gagaaactgc tcctctggtc
    31501 tggtattacc tttggcttgg ggcaaatcgt gattgccttg tcttcatcct tgtgggtggt
    31561 agtagcagga agtgttctgg ctggatttgc ctatagtgta gtcttgacga cggtctttca
    31621 acttgtctct gaacgaattc cagctaaact cctcaatcaa gcaacttcat ttgctgtatt
    31681 aggctgtagt ttcggagcct ttacgacccc attcgttcta ggtgcaattg gcttactaac
    31741 tcacaatggg atgttggtct ttagtatctt aggaggttgg ttgattgtaa tctctatctt
    31801 tgtcatgtac ctacttcaga agagagctta ggattgattc ctaagttttt cttttgatac
    31861 tcaatgaaaa tcaaagagca tagttgattg agtttggaat agtacgctgt agctgctaaa
    31921 atatttctag aaattaattt gactttccta atagagttgt tcatatctta tttcaatcta
    31981 ctatagaaag ctagccgcag gttgctcaaa atagtgtttt gaggttgcag atggaaactg
    32041 acgtggtttg aagagatttt cgaagagtat gagattttta tacccataca atatttgttt
    32101 taggtccttg tttacagact aatttcttga taaaatagag gttatgacga gatataaagc
    32161 aactatttcc tatgatggtt atgcctttgc tggctttcag cgccagcctc atgcgcgtag
    32221 cgttcaggaa gaaattgaaa aaaccttgac cagattaaat aaagggcaaa ccattactgt
    32281 tcacggtgct ggtaggacag atagtggggt tcatgccctg ggacaggtca ttcattttga
    32341 cctgccttat cagatggatg aggagaaact ccgttttgcc ttggataccc agtctcctga
    32401 agatattgat gtgatttcga ttgagcttgt ggcagctgat tttcattgcc gttatgccaa
    32461 gcatagcaag acctatgagt ttattgtgga tagaggacgt cccaaaaatc cgatgcgccg
    32521 tcactatgcc acccactttc cctacccact cgatgtggaa cgaatgcaga ttgcaatcaa
    32581 aaagctagag ggaacccatg attttaccgg ttttacagcc tctgggacta gtgtagagga
    32641 taaggttcgc accatcacag aagctagttt aatagtcgat gagacaggac aatttttgac
    32701 ctttaccttt tcaggaaatg gtttcttgta taaacagatt cgcaatatgg tggggactct
    32761 tctcaaaatc ggtaacaacc gcatgccagt agagcagatt gatctcatct tggagaagaa
    32821 ggacaggcaa cttgcaggtc ccactgcagc accaaatggt ttgtatttaa aggagattcg
    32881 ttatgaagaa taatcgtatt ttagcacttt ctggaaatga tatttttagt ggtggtggac
    32941 tgtcagctga tttggctacc tataccttga acggcttgca tgggtttgta gcagtgactt
    33001 gtttgacagc cttgacagaa aaaggatttg aagtctttcc aactgatgat accatttttc
    33061 aacatgaatt agatagcttg cgtgatgtgg aatttggggg aattaagatt ggtcttctcc
    33121 ctactgtcag tgtggctgag aaggccttgg actttatcaa acaacgccca ggagtacctg
    33181 tggtgttgga tcctgtcttg gtctgcaagg aaacgcatga tgtagctgtc agtgacctct
    33241 gccaagagtt gattcgcttt ttcccttatg tcagtgtgat tacgcctaat ctcccagaag
    33301 cagaattatt atccggtcag gaaattaaaa ccttggaaga catgaaaact gcagcgcaga
    33361 aattgcgtga tttaggagcg ccagcagtca ttatcaaggg aggcaatcgt cttagtcagg
    33421 acaaggctgt ggatgtcttt tatgatggac agacctttac tatcctagaa aatccagtta
    33481 tccaaggcca aaatgctggt gcaggttgta cctttgcctc tagcattgcc agtcacttga
    33541 ttaaaggtga taaacttttg ccagcagtag aaagctctaa ggctttcgtt tatcgtgcta
    33601 ttgcacaagc agatcagtat ggagtaagac aatatgaagc aaacaaaaac aactaaaatc
    33661 gcccttgtat ccctattaac cgccctttct gtggttctag gttatttctt aaaaatccca
    33721 acacctacag gaattctaac tcttttagat gctggtgtct tctttgcggc cttttacttt
    33781 ggtagtcgtg aaggagcggt agtcggagga ctagcaagtt tcttgattga cctcttatca
    33841 ggctaccctc agtggatgtt ctttagcttg gtcaaccatg gcttgcaggg atttttcgca
    33901 ggatttaaag gaaaaagtca gtggttaggc cttattttag caactattgc catggtagga
    33961 ggctacgcct tgggttcgac tttgatgaat ggctgggcag cagccctccc agaaattcta
    34021 ccaaatttca tgcaaaatat ggtagggatg attgtaggat ttattcttag tcaaagtatc
    34081 aagaagatta agtaatactc ttcgaaaatc tcttcaaacc acgtcagttt ccatctgcaa
    34141 cctcaaaaca ctgttttgag cagcctgcgg ctagctttct agtctgattt tcattgagca
    34201 taaagaggtt gagtaaaaag tctttcaaat aagaaaaacg catagtatca ggtgttgaat
    34261 gaacacctga tactatgcgt tttattgtgg gaagattttc ctcattttct agtgaaattg
    34321 aactttttct aacatctttt gatagatgac ttcttaatca attatcattt ccattgttgt
    34381 ttcttggggt ttcatccctt gtctttgata aaagcggaga gcaccctcgt tatcattcca
    34441 gacacttagg gttagattat tgcagccaat ttctttagca taatagcaga aaaatgacaa
    34501 taaaatagcc cctagatatt ttgaaaggaa agcatagggg ctatatagtt taatcaaaat
    34561 aaagcaaatc tttgctggtg cttgtaaaga ggtcgaagcc atccttggta acaacaccgc
    34621 agtcttcaat acgaacaccg actttaccag ggatatagat acctggttca acagagaagc
    34681 acatgccttc ttcgatgacc atatcgtttc cttccatgat agatgggaat tcatggacat
    34741 ccataccgat accatgcccg agacggtggt tgaagtactc accataacca gctttttcga
    34801 tgacctcacg ggcagcgcgg tccacttcat gagcagtcac acctggcttg ataaagtcaa
    34861 gagcagcttg ttgggcttca agagtcaagt tgtaaatatc tttcttgaat tggtctggtt
    34921 tgccgacagc gactgtacga gtcatatctg acgcatagcc attgaccaga acacccaggt
    34981 caaagaggag aagagcatca ttttcaacct tattagctgc tggaatgccg tgtggattcg
    35041 cagcattatc accagtcaag accatggtat caaagctcat ttcataacct tcacgtttca
    35101 tggcaaagtc gatttgtgcg atgatatctg tctcagtctt atcaagagaa atattgtcaa
    35161 aaccaacatg aacagccttg tcagcataaa gacctgcaac catcattttt tgcacttcat
    35221 cagctgattt gatgaggcgc atgcgttgga tacgaggagt gaggttgtca aactcagcag
    35281 tctcaaaaac tgttttcaaa ccatggtatt tggtcaagat gagattgtca aactcaacag
    35341 cgacacgttt gaagtcaagt tgtggaagag catgtttgat tttttgccat ggattttcag
    35401 aatcgacata gcccactact gggaaggaaa cggtgctact tgcacgttct acttcaagag
    35461 ctgggacaaa gaggagaggt tcctgatccg ctaggacaaa gaggaacatt tggcgttcat
    35521 ggggatcact gtaaaaacca gtgaggtaat tgattgtgac ggggtcagat acgacagcga
    35581 cgtctagttt ttctgattca agatatgtta ggatttgttg taatttagac atatgctacc
    35641 ttctttctac ccctctattt tggcaaaaaa tgagagaaaa tgcaagggag aagggtaaaa
    35701 gaaaagacaa aaaactccga caagataacg gagttctttg atatcatttt cctttgttta
    35761 agaaaagtca gccttgcttt tgacgtcact gtactcttca gcgatttctt ggctgagaat
    35821 gtcctcatag gcagggagtt ggatgtcctg accatatttc ttcttgaggg cagtagtaac
    35881 taggcgataa gccagattgg cattacgaga gaggataggc ccgtggaagt aggaaccaaa
    35941 gacattctta taatgaaccc cttcaccgac cttttcttcg ttgtttccat ttccatagac
    36001 aacctgtccc agcggttttt ggtcatcaga gaggaaggta cgaccttggt gattttcaaa
    36061 tccatagtag gtttcatcga aatcttcatt gtgaatcttg atgtcaccga taaaacggtt
    36121 attggtctgg ttgagcgtgt agtgtcccat gacccctagc ccttcgatac gttttcctga
    36181 agcttcaaca taatattgac ccaatagttg gaaaccaccg cagatagcca gaactacacc
    36241 gtcgttttgg atgtagttgt caatgctctc ttttttagca ggtaggtcgt ctgcaatgat
    36301 actttgttca aagtcttgac caccaccgaa aaaggcgatg tcgtagtgat tttcatcaaa
    36361 gtcatcatgg agagaaacga tgtcaacggt cacatgggct cccagttttt cagccacata
    36421 cttgagcatg aggatgtttc cattgtcccc gtaggtattc atgagatttc cgtagaggtg
    36481 ggcaatgttg agctgatagg ggtaattgct atcttttgag gaaagtgaag tataaaccat
    36541 tagttcatct cctttctaac aatctgacga ctagccagca gttcacgaaa ttccagcatg
    36601 gcagtataag ttgccagaat ataggcatgc ttgcagtctt gattctcaat ggtcttgaga
    36661 acttgctcca gattactcgt ttcagtgatt ttctcagctg gatagccagt cactcggagg
    36721 cgacgagcga tttcagaatg acgaacaccg ccagcgttga tttcaggaat gtccatgtca
    36781 gtgatttgct caaagtctgc atcccagatc cagctagtgt caattccatc tgcatagttg
    36841 gcattaagga ggacagatag gctaaatgga taaggtgcta gtttgatcat ttcgatagct
    36901 tgggttgcac cgactggatt tttaatcaag acaagggtac attccttgtc accgatatga
    36961 aaggtttctt ggcgtccaaa gacagcacgg ctcttgtcaa atccctgttt gatgagttgg
    37021 gaatctgcgc cgaggaaacg ggcgatggcc acagcagcta gggcgttata gatattatag
    37081 agcccgccga tttggatacc gtattcttgg ccgtctatga caaagcgaga gcgattgttg
    37141 gtcaactcaa ccagttttgt caaacgatag tcgagatcag gacgtttaca tccacaacct
    37201 tcacagatat aggcacccaa gtttgcatag gtattatgct catatttgag gatgccttgg
    37261 cagtcaggac agagaatccc ttcggtattg tagtgagcca gttgggctgg tcctttttcc
    37321 aagtcaaaac caaaatactc tatagggttt ggaatagttg gcttgtagaa aagtggactg
    37381 tctccgttaa ggagaacagt agcagttgga acttttcgaa tggcatccaa tatcatgtta
    37441 taggtagtat agatttcacc gaaacggtcc atctggtcac ggaagatatt agtaatgaca
    37501 aaaagactag gctggatata gtcacagata cgagatagac tggcttcgtc aatttcgagg
    37561 acggcaatat ttttcccagt ttttgaagat ttggctgtta ggaaggttgt tgcaatccct
    37621 gtaatcatgt tggcaccgct tgggttggtt agaacttgac cataaacctc ttttaaaatg
    37681 ccgacagtga gggcagttgt cagggttttt ccatttgttc cagtgacaac gacaatctcg
    37741 tagttcttag ctaggctttg taaaatatct ttatcaaatt gaagggcgac tttccctggg
    37801 agcgtacttc cacgtccaag acggcttaaa acgaagtggg aagaacgccc agcaagaagg
    37861 cccaaagtag tttttaagtt catgtttcta ttatatcaca aaagagggaa aagacagatt
    37921 tggtgtttcg tagaagtaaa aacagcccaa agagggctgt cagttaagat gagatttcaa
    37981 cttaaatcgc aaacaagtca ttcaagttct cagccaaggt tgggtgagtg aagatttgtt
    38041 ttgtgaagta agtgtaagga atcttgttgt ccatagcaac agtaatgatg ttgatgattt
    38101 cttgagaacc ttctgagaag atgcttgcac caagaatttc ttttgtttca gtattaacaa
    38161 cagctttgaa agctccgcga aggtctccat ttacgtgacc acgaggcatg gctgcaacag
    38221 ggatttcttt cactgcgtat ggaagtttca aatcagctgc ttggctttca gtcaaaccaa
    38281 cttgtgaaag tgcaggtgtg atgaacatag tatttggcac attgagacgg tcctcaagtg
    38341 tgtagctgcc atctccagca aggtagctgt aaacaacacg gaagtcatca agtgaaatgt
    38401 aagtaaattg aaggccaccg ttgacatctc caactgcaaa gacaccagga acgtttgttt
    38461 gacagtgttt gtctacttta atagcaccac gttcagttag ttcaatatct gtattttcaa
    38521 gttgaagtgg ttctacattt ggtttgcgtc cagttgcgta gagaagggcg tcgaaacggt
    38581 aagtttcgtc ttcagttacg acaagcactt ggtcaccatc gtttttgatt tcagtagtat
    38641 ggatattttg aagcaattca atgccatctt cttccatgta ttgtttagca agagctgcga
    38701 tggaaggttc tgcacgaggt aggaatgtat ccaaggcatc taggactgtg accttgcttc
    38761 caagtttgtt gtaaaggccg gcaaattcaa gaccgatatt tccgccacca aggattccaa
    38821 gtttttcagg taatttgtcc aagctttgga tacctgttga gtcaaagatg tttttgcttg
    38881 tagcaagtcc aggaattggc aagacgtttg aaacagcacc agtgttgatg acgattgttt
    38941 cagcagtcag ttctttcttt tcatcaccag cttggatttc gatgacttta tttgaaagga
    39001 agtgagcttc cgcatcaaag atatctacgc ctgtaccagc aacagtcgca tagtttttac
    39061 cgttgaggcg accagtgatc gtgtttttag tagcaatcac ttcttcaaaa gacaagtcct
    39121 tttcagcagc aactagcaag gttttagttg ggatacaacc aatgttgata caagttccac
    39181 cgtacatagc cttgctgcgt tcaacgaggg caactttttt gccagctgaa gccaatttac
    39241 ctgctagtgt tttaccagct ttaccaaatc caataacaat taaatcgtat gttaacattt
    39301 atcttcctcc tattcgaatt tcattctagc acaagaaaaa aacttttgca caattctgct
    39361 acggaaaaga cagaaaaaga taaaaacgct ttccagacaa aaaactagta attttttgac
    39421 aagcttgata aactaaaatc ctatctattt ttatataaga aagaataaat gttcgaaaat
    39481 aggaggtttt atgaaatcga atcgttatat tattgccttt gctggggtca ttttacactt
    39541 aatgctgggt tcgacttatg cttggagtgt ttatcgtaac cctattattg aaaaaacggg
    39601 atgggatcag gcttctgttg ccttcgcctt tagtctagca atcttttgtt tgggcttatc
    39661 ggctgcattt atggggcgtt tggtagaaaa atttggtccg aaagtcatgg ggagtctatc
    39721 tgcttttcta tacgcaggtg gaaatatctt aacaggattt gcaatagacc gtcaggaact
    39781 gtggttgttg tatctagctt atggcatttt aggtgggctt ggtttgggag caggctatat
    39841 tacccctgtg tcaacgatta taaaatggtt tcctgataaa cgtggtctcg caacaggttt
    39901 agcgattatg gggtttggtt ttgcttcttt attgactagt cccatagcgc aacacctcat
    39961 cgcaggggta gggcttgtag aaacttttta tattttagga gcaagttact ttattatcat
    40021 gctcctagct tcacaattca ttaagcgtcc aaatgagcaa gagcttgcaa ttttatcttc
    40081 ttcagggaaa gaaaaaacag cctctttgac gcaaggaatg gctgcaaatc aggctctaaa
    40141 aagcaatcgg ttttatatgc tttggattat tttctttatc aacatagctt gtggtttagg
    40201 cttaatttca gcggcatcgc caatggcaca ggagatggct ggcttgtcta caagtcatgc
    40261 agcagtaatg gtgggtgttt tggggatttt caatggattt ggtcgcttgc tctgggcgag
    40321 tttgtctgac tatatcggtc gccctctaac ctttagtata ttactgcttg tcaatctttt
    40381 cttttctctc tcactttggc tctttacaga ttccgtttta tttgtagttg ctatgtctat
    40441 tttgatgact tgctatggag ctggtttttc tttgattcca gcttatctca gtgatatttt
    40501 tggaaccaag gaattggccg ctctgcatgg ttatatttta acagcttggg caatggctgg
    40561 tttagcggga cctattttat tagcagagac ttataaaatg gctcattcgt acacacaaac
    40621 cttgttcgtt tttctcattt tatacagtat cgccttggct ttgtcttatt atctaggtcg
    40681 ttcaatcaaa aaagaaagtc aaaaaccgct tacatgattt tctgtagaaa tggggctatc
    40741 ttttgctctg gtcttgtgtt atactagata ggttgcaaag aaaacagtac ttttcttttg
    40801 tggaaaagaa gcaacacgat tttatatcta gttatatgaa aatacgtcat aaaaagaaaa
    40861 gtataaacta agccctatag ggtggcttcg caccaccttt agaaagaaga ataacgtgaa
    40921 atttaatgaa ttaaacttgt ctgctgattt gctagcagaa attgaaaaag ctggttttgt
    40981 agaagctagt cctatccaag aacaaactat tcctttggcc cttgaaggca aggatgttat
    41041 cggtcaagct cagactggta caggaaaaac tgcagccttt ggcttgccta cccttgaaaa
    41101 aatccgtaca gaagaagcga ctattcaagc cttggtcatc gctccaactc gtgaactagc
    41161 tgtccaaagt caagaagaac tcttccgctt tggtcgtagt aagggagtca aagtccgttc
    41221 agtatatggc ggatcaagca ttgaaaaaca aattaaggct cttaaatctg gtgcccatat
    41281 cgtggtggga actccaggtc gcctcttgga cttgattaaa cgcaaggcct tgaaattaca
    41341 agacattgaa acccttatcc ttgacgaagc ggatgaaatg cttaacatgg gcttccttga
    41401 agacatcgaa gccattattt cccgtgtacc tgagaaccgt caaactttgc ttttctcagc
    41461 aactatgcca gatgccatca aacgtatcgg tgttcagttt atgaaagccc ctgaacatgt
    41521 caagattgcg gctaaggaat tgacaacaga attggttgac cagtactata tccgtgttaa
    41581 ggaacaagaa aaatttgaca ccatgactcg tctcatggat gtggcacaac cagaactcgc
    41641 tattgtattt ggtcgtacca aacgccgtgt ggatgaattg actcgtggtt tgaaaattcg
    41701 tggcttccgt gcagaaggaa ttcatggcga cctagaccaa aacaaacgtc ttcgtgtcct
    41761 tcgtgacttt aaaaatggca atcttgatgt tttggttgcg acagacgttg cagcgcgtgg
    41821 tttggatatt tcaggtgtga cccatgtcta caactacgat attccacaag atcctgagag
    41881 ttatgttcac cgtatcggtc gtacaggtcg tgctggtaag tcaggtcaat ctattacttt
    41941 tgttgctcca aacgaaatgg gttaccttca aatcattgaa aacttgacta agaaacgcat
    42001 gaaaggtctc aaacctgcaa gtgtagaaga atccttccaa tcaaaaaaac aggtagttct
    42061 caagaaaatc gaacgtgatt ttgcagatga aaccattcgt gccaactttg agaaatttgg
    42121 taaggatgct cgcaaattgg ctgctgagtt tactccagaa gaattggcaa tgtatatctt
    42181 gagtctgaca gtccaagacc cagatagcct tccagaagtg gagattgcac gtgaaaaacc
    42241 actaccgttt aaaccatcag gtaatggttt cggtggtaaa gctaagggag gtcgtggagg
    42301 ccgtcgtggg gacgaccgtc gagagcgtga tcgtcgtggc aatggtcgcc gtgatgagtt
    42361 caaaaaagga agtcgtggca acgatcgttt tgataaggaa aaacgttacc gtaaggataa
    42421 taaaaaacca cgcaatactt taagcgaaaa gcaaacaggc tttgttattc gtaacaaagg
    42481 tgataaataa gaaaaagcgg ttcaaaatga atcgcttttt tatataagga gaccgaatgt
    42541 gaaaaagaca aataaaatta ttatattatc ttttataata ttatgataca caaaaatagg
    42601 cgacttgtca atagaaaagg aacaatttaa ttaaaaatat tctggttttt aattaaattg
    42661 taagtcaatg caagtcaata aggaattttc agataattat tgaaaaagag acgtttttat
    42721 gttataatga tagcgattaa attcgaggtg aaaaatggca catttattag aaaaaactag
    42781 aaaaattaca tcaattttga aacgctcaga ggagcagttg caggatgagc tcccttacaa
    42841 cgctattacg cgtcagttag cggatattat tcattgcaat gcctgcatta tcaatagtaa
    42901 gggacgtctg cttggctatt ttatgcgtta taaaacaaat acagatcgcg tagagcaatt
    42961 cttccaaact aagattttcc cagatgacta cgttcaaggg gctaatatga tttacgaaac
    43021 agaagcaaac ttgcctgttg agcatgatat gagtattttc cctgttgaga gtagagatga
    43081 ttttccagat ggcttgacga ctattgcacc gattcatgta tcggggattc gccttggttc
    43141 tttgattatt tggcgtaatg ataaaaaatt cgaagatgag gacttggttc ttgttgagat
    43201 tgccagtacc gttgttggga ttcagcttct taacttccaa cgtgaagaag atgagaaaaa
    43261 tattcgtcgt cgtactgctg tcaccatggc ggtcaatacc ctttcttact ccgaactccg
    43321 tgctgtttca gcaattttag gggaattaaa tggaaatgaa gggaagttga ctgcgtcagt
    43381 gattgcagat cgtatcggaa tcactcgctc tgtgattgtc aatgctcttc gtaaacttga
    43441 gtctgcgggg attattgaaa gtcgctcact tggaatgaag ggaacctatc ttaaggtctt
    43501 gatttcagat atttttgaag aagtgaagaa aagagattac taatgacaaa ggctttaatt
    43561 tcgattgatt atacagaaga ttttgttgct gatagtggga agctgacagc aggtgctcca
    43621 gctcaggcga tttcggatgc catcagcaag gtaactcgat tagcttttga acgaggagat
    43681 tatatcttct ttactattga tgctcatgaa gaaaacgatt gtttccatcc ggaaagtaag
    43741 ttatttcctc ctcataatct gattgggacg agtggacgga atttatatgg agatttgggg
    43801 atcttttatc aagagcatgg ttcagacagt cgtgtctttt ggatggataa acgccattac
    43861 tcagcttttt cagggactga cctagatatt cgtttgagag agcgtagagt gtctaccgtt
    43921 atcttaacag gtgtcttgac ggatatctgt gtcctacata cagctataga tgcctataat
    43981 ctaggatatg acatcgagat tgttaaacca gctgttgctt ccatctggcc tgaaaatcat
    44041 caatttgccc taggtcattt caaaaataca ctcggagcta agttagtaga tgaaaatcta
    44101 aatgaacttt ctgagtaacc tggttggtga aaatgaaaaa atttgaaaaa aagtgttgac
    44161 aagcatccca aaagttgata tactagtaaa gtaatcgatg cggggatggc ggaattggca
    44221 gacgcgcagg actaaggatc ctgtgaccgc tttaggtcgt gagggttcaa gtccctctct
    44281 ccgcatagga aaagagttag ctaaagctag ctcttttgta tttctctaaa aaattagaat
    44341 ccagtcaaat gtagtggaga atcagcgaaa atatgggaaa actgatcctt gtttctgctt
    44401 ttgtcataga agaataataa agattatctt cttcaaattc tccgatattc tctaaagttt
    44461 tgtgcaagtt gcacagaact tttttatttt tttggtcatc ttgccataga aatataaagc
    44521 gttttcatct ataatataat tatcaaaaga caaaaggagt tcacctcatg gtagaattga
    44581 atcttaaaaa tatttacaaa aaatatccaa acagcgaaca ctattcagtt gaagatttca
    44641 acttgaacat caaagataaa gaatttatcg ttttcgtagg accttcagga tgtggtaaat
    44701 caactacact ccgtatgatt gctggtcttg aagacatcac agaaggtact gcatctatcg
    44761 atggcgtggt tgtcaacgac gtagctccaa aagaccgtga tatcgccatg gtattccaaa
    44821 actacgctct ttacccacac atgactgttt atgacaacat ggctttcggt ttgaaattgc
    44881 gtaaatacag caaagaagac attaacaaac gtgttcaaga agcagctgaa atacttggat
    44941 tgaaagaatt cttggaacgt aaaccggctg acctttcagg tggtcaacgt caacgtgttg
    45001 ccatggggcg tgcgattgtc cgtgatgcga aagtattctt gatggacgaa cctttgtcaa
    45061 acttggatgc caaacttcgt gtatcaatgc gtgctgaaat cgctaaaatt caccgtcgta
    45121 tcggagctac aactatctat gtaactcacg accaaacaga agcgatgaca cttgcagacc
    45181 gtatcgttat tatgtcagct actaagaacc ctgctggtac aggtactatc ggacgtgtag
    45241 aacaaatcgg tactcctcaa gaagtttaca aaaatccagt taacaaattc gttgcaggat
    45301 tcatcggaag cccagctatg aacttcatca acgtgaaatt ggttggtagc gaaattgttt
    45361 ctgacggttt ccgtttgaaa gtgccagaag gagcattgaa agttcttcgt gaaaaaggct
    45421 acgaaggaaa agaattgatc tttggtatcc gtccagaaga cgtgaatgca gaacctgctt
    45481 tccttgaaac attcccagac tgtgttgtaa aagcgactat ctctgtatca gaactgcttg
    45541 gttcagaatc tcacctttac tgccaagttg gtaaagacga gtttgttgca aaagttgatg
    45601 ctcgtgacta cttgcaaaca ggtgcaacag ttgagcttgg atttgacttg aacaaagcac
    45661 acttcttcga tgtagaaact gaaaaaacaa tctactaaga ataaataaaa ttcaaagcac
    45721 tacaagaaaa gatatctctt tatcaattgt agtggagaga tatcagttaa tctagggaga
    45781 gaaacaaaat gcttctctcc tttttgctag ggaagtcata ttatacatct atattgtgat
    45841 gctctttata ctcttcgaaa atctcttcaa accacgtcaa cgtcgccttg ccgtacgtat
    45901 gattactgac ttcgtcagtt ttatctgcaa cctcaaagct gtactttgag tagcttacgg
    45961 ctagtttcct agtttgctct ttgatttcca ttgagtatta tttgtgggta ccatctacaa
    46021 gtgaagctat atgcgtaaac tacgtgagca attgaattcg aactagagag gtaataataa
    46081 atttatgcta tagttatggt gacttgtatg cttttgattc tagtttatca aataatagat
    46141 tagaattgtc agataatatc attttgtgtc attttgtgtt ataatgaaga aaaaacagag
    46201 gtgttcaaat gtcagaagca ggtcataagt ttttagcaaa attggggaaa aaacgcttac
    46261 gtccaggtgg aaagcgtgcc acagattggt taattgcaga aggaggattt tcaaaagaaa
    46321 agagaatact agaggttgcg tgtaataggg gaactacagc aattgagttg gcacagcgtt
    46381 ttggttgcaa gataactgct gttgatatgg atgctcaagc tttagaagtg gctaaaaaat
    46441 ctgctggaac ggcaggtgtt gctcatttaa tcagttttga aagagcaaat gcaatgaaac
    46501 ttccttatca agatgctagt tttgatattg ttataaatga agctatgctg actatgcaag
    46561 ccgatcaagc taagaaaaaa tgtgtaatgg aatatctaag ggtattaaaa cctggaggtc
    46621 ttctcttgac acatgatgtg cttcttaagg aagctaaaga gtctatcaga caggaattat
    46681 cacaagcaat tcatgtaaat gtaggtcctt taactcaaga tggttgggaa caggtgatga
    46741 tagaatcagg ttattgtgat gtgaaagtat tgactggtga aatgacatta atgaaattat
    46801 cgggtatgat ttatgacgaa ggttggctag gaactttgaa aatttgtgta aatgcttgta
    46861 aaaaggagaa tagaaagcag tttttaacta tgtataaaat gtttgctaag aataaacaga
    46921 aattgggctt tattgcgatg gctagttata aatcgtcaaa acgttagata attattgaag
    46981 ttaacttttc cttttttctt tcttaaaaaa tatgctataa tagagagtaa aaaactttga
    47041 aagaaagaaa aagatgaatt taaaagatta cattgcaaca attgaaaatt atccaaagga
    47101 aggcattacc ttccgtgata ttagtccttt gatggctgat ggaaatgctt atagctacgc
    47161 tgttcgtgaa atcgttcagt atgctactga caagaaagtc gacatgatcg tgggacctga
    47221 agctcgtgga tttatcgtgg gttgtccagt tgcctttgag ttgggaattg gttttgcgcc
    47281 tgttcgtaag ccaggtaaat tgccacgcga agttatttct gctgactatg aaaaagagta
    47341 cggtgtcgat accttgacta tgcacgcgga tgccattaag ccaggtcaac gtgttcttat
    47401 tgtagatgac cttttggcga caggtggaac tgttaaggca actatcgaga tgattgaaaa
    47461 acttggtggt gttatggcag gttgtgcctt ccttgttgaa ttggatgaat tgaacggccg
    47521 tgaaaaaatt ggtgactacg attacaaagt tcttatgcat tattaatgaa aacagtccct
    47581 agggctgttt tctctacact aggatataaa aatagactat aactagttag agaaaaacta
    47641 taattgaaaa ctatatcttc ttacagtata ataaaaggac taagtgtttg agatttgtct
    47701 tcaaacatat gcaattattc ctgaaagagt acagttagga gagggttatg ccgattcgaa
    47761 ttgataaaaa attgccagct gttgagattt tacggacaga gaatatcttt gtcatggatg
    47821 atcaacgtgc tgcccaccaa gatatccgtc ctttgaagat tttaatttta aatctcatgc
    47881 cacagaaaat ggtcacagag acccagttgt tgcgccactt ggctaataca cccctacaac
    47941 tggatattga ttttctctat atggagagcc accgttctaa aacaactcgt tcagagcaca
    48001 tggagacctt ctataaaact tttcctgaag tcaaggatga gtattttgat gggatgatca
    48061 tcacgggtgc tccagttgag catttaccat ttgaggaagt ggactattgg gaggaattta
    48121 gacagatgct tgagtggtct aagactcatg tctattcgac ccttcatatc tgttgggggg
    48181 ctcaggctgg gctttatctg cgctatggtg tagaaaaata ccagatggac agtaagctat
    48241 caggtattta tcctcaggac accctaaaag agggtcacct tctatttaga ggctttgatg
    48301 atagctatgt atcccctcat tcacggcaca cggagatttc taaggaagag gtcttaaaca
    48361 agaccaatct cgagatttta tcagaaggac ctcaggttgg ggtttctatt ttggccagtc
    48421 gtgatttacg agaaatttat agttttggtc atttggagta tgaccgtgat accttggcaa
    48481 aagagtattt tcgagatcgt gatgcaggtt tcgacccaca tattccagaa aattacttta
    48541 aagatgatga tgttaatcag gtaccttgtc tttgttggtc ttcatctgca gccctctttt
    48601 tcagtaattg ggtaaaccat gcggtctatc aggagacgcc ttttgactgg agaaagatag
    48661 aagatgatgc atctgcatat aggtatttat aagaggaatt atgacatatt tagacgcttt
    48721 taaatcaggt accttggttt taccgagtgc cctgctcttg cattttaagg aactctttcc
    48781 ttctagcgac gattttctgg tttggcaatt tttctatttg caaaatacga caggcttaga
    48841 agaaatatcg ccaagccaga ttgctgaaag gattggcaag gaaatttcgg atgtcaacca
    48901 gtccatttct aatctgacgg aaaggggact gctccagtat cgtactatcg aattaaatgg
    48961 cgaaattgaa ttgctctttg atgctagttt ggccttggaa cgtttggatg acctgtttgg
    49021 agcagttcat tcaagttcag accagctaac accacaaaac cagctcaagg atttggtgga
    49081 aaccttccag caggagttgg gacgattgtt gacgcctttt gagattgagg atttgaccaa
    49141 gacactaaag gaagatggaa ccagtgctga cttgattaag gaggctcttc gtgaagctgt
    49201 tttgaacgga aaaccaaact ggaagtacat tcaggcgatt ttgagaaact ggcgccatga
    49261 aggaatcaag agtgtggctc aaattgaggc caagagagca gaaagagaag caagcaatcc
    49321 tcagttgaca caggtatctg cagatttcat aaatgccatg gatctctgga aggattaatc
    49381 catgcaagta ggcttgaaat ccgagtaaga tttgcaagct gtgtataatt gtgatagaat
    49441 aaatagaaaa taaattgaaa aaagaggtat gtgaaatgtc acgtaaacca tttatcgctg
    49501 gtaactggaa aatgaacaaa aatccagaag aagctaaagc attcgttgaa gcagttgcat
    49561 caaaacttcc ttcatcagat cttgttgaag caggtatcgc tgctccagct cttgatttga
    49621 caactgttct tgctgttgca aaaggctcaa accttaaagt tgctgctcaa aactgctact
    49681 ttgaaaatgc aggtgctttc actggtgaaa ctagcccaca agttttgaaa gaaatcggta
    49741 ctgactacgt tgttatcggt cactcagaac gccgtgacta cttccatgaa actgatgaag
    49801 atatcaacaa aaaagcaaaa gcaatctttg cgaacggtat gcttccaatc atctgttgtg
    49861 gcgaatcact tgaaacttac gaagctggta aagctgctga attcgtaggt gctcaagtat
    49921 ctgctgcatt ggctggattg actgctgaac aagttgctgc ctcagttatc gcttatgagc
    49981 caatctgggc tatcggtact ggtaaatcag cttcacaaga tgatgcacaa aaaatgtgta
    50041 aagttgttcg tgacgttgta gctgctgact ttggtcaaga agtcgcagac aaagttcgtg
    50101 ttcaatacgg tggttctgtt aaacctgaaa atgttgcttc atacatggct tgcccagacg
    50161 ttgacggtgc ccttgtaggt ggtgcgtcac ttgaagctga aagcttcttg gctttgcttg
    50221 actttgtaaa ataatcagta agtagcaaaa gctaggtgga acagcattca gatgtctgtt
    50281 acatttttta taggagagga aagattgaaa acaaaaattg gattagcaag tatctgttta
    50341 ctaggcttgg caactagtca tgtcgctgca aatgaaactg aagtagcaaa aacttcgcag
    50401 gatacaacga cagcttcaag tagttcagag caaaatcagt cttctaataa aacgcaaacg
    50461 agcacagaag tacagactaa tgctgctgcc cactggtatg gggattatta tgtaaaggat
    50521 gatggctcta aagctcaaag tgaatggatt tttgacaact actataaggc ctggttttat
    50581 attaattcag atggtcgcta ctcgcagaat gaatggcatg gaaattacta cctgaaatca
    50641 ggtggatata tggcccaaaa cgagtggatc tatgacagta attacaagag ttggttttat
    50701 ctcaagtcag atggggctta tgctcatcaa gaatggcaat tgattggaaa taagtggtac
    50761 tacttcaaga agtggggtta catggctaaa agccaatggc aaggaagtta tttcttgaat
    50821 ggtcaaggag ctatgatgca aaatgaatgg ctctatgatc cagcctattc tgcttatttt
    50881 tatctaaaat ccgatggaac ttatgctaac caagagtggc aaaaagtggg cggcaaatgg
    50941 tactatttca agaagtgggg ctatatggct cggaatgagt ggcaaggcaa ctactatttg
    51001 actggaagtg gtgctatggc gactgacgaa gtgattatgg atggtgctcg ctatatcttt
    51061 gcggcctctg gtgagctcaa agaaaagaaa gatttgaatg tcggctgggt tcacagagat
    51121 ggtaaacgct atttctttaa taatagagaa gaacaagtgg gaaccgaaca tgctaagaaa
    51181 atcattgata ttagtgagca caatggtcgt atcaatgatt ggaaaaaggt tattgatgag
    51241 aacgaggtgg atggtgtcat tgttcgtcta ggttatagcg gtaaagaaga caaggaattg
    51301 gcgcataaca ttaaggagtt aaaccgtctg ggaattcctt atggtgtcta tctctatacc
    51361 tatgctgaaa atgagaccga tgctgagaat gacgctaaac agaccattga acttataaag
    51421 aaatacaata tgaacctgtc ttaccctatc tattatgatg ttgagaactg ggaatatgta
    51481 aataagagca agagagctcc aagtgataca gacacttggg ttaaaatcat caacaagtac
    51541 atggacacga tgaagcaggc gggttatcaa aatgtgtatg tctatagcta tcgtagttta
    51601 ttacagacgc gtttaaaaca cccagatatt ttaaaacatg taaactgggt agcggcctat
    51661 acgaatgctt tagaatggga aaacccttat tattcaggag aaaaaggttg gcaatatacc
    51721 tcttctgaat acatgaaagg aatccaaggg cgcgtagatg tcagcgtttg gtattaagcg
    51781 atgatttgaa agagggatgt gatagtagca ccctcttttt ctttgtttta tgatagttca
    51841 tcctcgagta aattcaagat cttgctcgga aatgaagctt atatagtaga ttgaatatag
    51901 acaaatacct tgtgattggt aaaacatttt agaaattcat ttacctttcc taatcgactt
    51961 ggtttcatct tatttcaatc tattatagta ttggggaatt tcttcaaacc acatcagctt
    52021 ggtcagttct acctgcgacc tcaaaacttg tgctttggtc aagctgggtt tagtttccta
    52081 gtttgctgat ggatttccat tgactataag catccaaccc tctttttgtc ttctaaagaa
    52141 ttcttaaatt atcagtctat tgcaactttt ctcatataag ttctttgtct tgctattggt
    52201 tttccttagt agtatactaa ggtagtaatc attaagaagt ggttacaaaa aataatgaat
    52261 gaggtaaaga aaatggtaga attgaaaaaa gaagcagtaa aagacgtaac atcattgaca
    52321 aaagcagcgc cagtagcatt ggcaaaaaca aaggaagtct tgaaccaagc tgttgctgat
    52381 ttgtatgtag ctcacgttgc tttgcaccaa gtgcactggt atatgcatgg tcgtggtttc
    52441 cttgtatggc atccaaaaat ggatgagtac atggaagctc ttgacggtca attggatgaa
    52501 atcagcgaac gcttgattac actcggtgga agcccattct ctacattgac agagttcctt
    52561 caaaatagtg aaatcgaaga agaagctggt gaataccgta atgttgaaga aagcttggaa
    52621 cgtgttcttg ttatctaccg ttacttgtca gaacttttcc aaaaaggttt ggatgtcact
    52681 gatgaagaag gtgacgatgt aacaaacggt atctttgcag gcgctaaaac tgaaacagat
    52741 aaaacaattt ggatgcttgc agccgaactt ggacaagcac ctggtttgta agaaataagg
    52801 aaacatagat aaatctgtag gaaatttctt acagattttt tctattttgt aagctattcc
    52861 aaaccagtct ttttttgagt tttttgataa aatagtacta tcagtgaaaa ggatggaagc
    52921 atgactaaga aaatcgtagc tatttgggcc caggatgaag agggtgtgat tggtaaggaa
    52981 aatcgtctgc cttggcattt gccagcagaa ttgcagcact ttaaagaaac aactctgaat
    53041 catgctatct tgatggggcg tgtgaccttt gatgggatgg ggcgtcgctt gcttccaaaa
    53101 cgggaaaccc tgattttgac gcgtaatccg gaagaaaaga tagatggggt tgctactttt
    53161 caggacgtcc agtctgttct tgactggtat caggctcaag aaaagaatct ctacattatc
    53221 ggtgggaagc aaatttttca ggcttttgaa ccttaccttg atgaagtgat tgtcactcac
    53281 attcatgctc gggtggaagg agatacctat ttccctgaag aacttgactt gtctcttttt
    53341 gagacagttt caagcaaatt ttacgccaaa gatgagaaga atccttatga ttttaccatc
    53401 caataccgca agagaaagga agtctaatgg aacggagttt atttggtcta ttcacggctt
    53461 ttctgtgttt catctgcttt ctggcaggag cacaggcttt tcgtaaaaag cgttacgggc
    53521 tttctatcct cctttggtta aatgccttta ccaatctggt caatagtatt catgcttttt
    53581 acatgacctt attttagata gaatgacagt atagaatgga aggaaatcat gtctacaaat
    53641 agaaaaaatg atatgatggt ttattgctca ttttgtggca aaaaccaaga agaagtacaa
    53701 aaaataattg ctggcaacaa tgcttttatt tgtaatgaat gcgtggagtt agctcaggaa
    53761 atcattcgag aagaattggt tgaggaagtc ttggcagact tgtctgaggt gccaaaacca
    53821 attgaactcc tccatatctt gaaccactat gtaattggtc aagatcgtgc caagcgtgcc
    53881 ttggcagtgg cggtttataa ccactacaaa cgcatcaatt tccacgatac acgcgaagag
    53941 tcagaagatg tagatttgca gaagtcaaac attttgatga ttggcccaac tggttcaggg
    54001 aaaactttcc ttgcccagac cttggctaag agcttgaatg tgccttttgc tattgcggat
    54061 gcgacagctc tgacggaggc tggttatgtg ggtgaggatg tggaaaatat cctcctcaaa
    54121 ctcttgcagg ttgctgactt taacatcgaa cgtgcagagc gtggcattat ctatgtggat
    54181 gaaattgaca agattgccaa gaagagtgag aatgtgtcta tcacacgtga tgtttctggt
    54241 gaaggggtgc aacaagccct tctcaagatt atcgagggaa ctgttgctag cgtgccgcct
    54301 caaggtggac gcaaacatcc acaacaagag atgattcaag tggatacaaa aaatatcctc
    54361 ttcatcgtgg gtggtgcttt tgatggtatt gaagaaattg tcaaacaacg tctgggtgaa
    54421 aaagtcatcg gatttggtca aaacaataag gcgattgatg aaaacagctc atatatgcaa
    54481 gaaatcatcg ctgaagacat tcaaaaattt ggtattatcc ctgagttgat tggacgcttg
    54541 cctgtttttg cggctcttga gcaattgacc gttgatgact tggttcgcat cttgaaagag
    54601 ccaagaaatg ccttggtgaa acaataccaa accttgcttt cttatgatga tgttgagttg
    54661 gaatttgacg acgaagccct tcaagagatt gctaataaag caatcgaacg gaagacaggg
    54721 gcgcgtggac ttcgctccat catcgaagaa accatgctag atgttatgtt tgaggtgccg
    54781 agtcaggaaa atgtgaaatt ggttcgcatc actaaagaaa ctgtcgatgg aacggataaa
    54841 ccgatcctag aaacagccta gaggtgacta tggaacttaa tacacacaat gctgaaatct
    54901 tgctcagtgc agctaataag tcccactatc cgcaggatga actgccagag attgccctag
    54961 cagggcgttc aaatgttggt aaatccagct ttatcaacac tatgttgaac cgtaagaatc
    55021 tcgctcgtac atcaggaaaa cctggtaaaa cccagctcct gaactttttt aacattgatg
    55081 acaagatgcg ctttgtggat gtgcctggtt atggctatgc tcgtgtttct aaaaaggaac
    55141 gtgaaaagtg ggggtgcatg attgaggagt acttaacgac tcgggaaaat ctccgtgcgg
    55201 ttgtcagtct agttgacctt cgtcatgacc cgtcagcaga tgatgtgcag atgtacgaat
    55261 ttctcaagta ttatgagatt ccagtcatca ttgtggcgac caaggcggac aagattcctc
    55321 gtggtaaatg gaacaagcat gaatcagcaa tcaaaaagaa attaaacttt gacccaagtg
    55381 acgatttcat cctcttttca tctgtcagca aggcagggat ggatgaggct tgggatgcaa
    55441 tcttagaaaa attgtgagga aaagaaaatg gcaaaaacaa ttcatacaga taaggcccca
    55501 aaggctatcg gaccctatgt tcaagggaaa atcgttggca accttttgtt tgctagcggt
    55561 caagttcccc tatcccctga aactggggaa attgtaggag agaatatcca agaacagaca
    55621 gagcaagtct tgaaaaacat cggtgctatt ttggcagaag caggaacaga ctttgaccat
    55681 gttgtcaaaa caacttgctt cttgagcgat atgaacgact ttgttccttt taatgaggtt
    55741 taccaaacgg ccttcaaaga ggaattccca gctcgttcag ctgtggaggt agctcgtctt
    55801 cctcgtgatg taaaagtcga aattgaagtc atcgcagaga ttggataagc tagttgaagt
    55861 ttggtgttgc caaacttctt ttgatataag gagaaaaaga tgacaaagaa acaacttcac
    55921 ttggtgattg tgacagggat gagtggcgca gggaaaactg tagccattca gtccttcgag
    55981 gatctaggtt atttcaccat tgataatatg ccgccagctc tcttgcctaa gtttttgcag
    56041 ctggttgaaa ttaaggaaga caatcctaag ttggccttgg tagtggatat gcgtagccgt
    56101 tctttctttt cagagattca agctgttttg gatgagttgg aaaatcaaga tggtttggat
    56161 ttcaaaatcc tctttttgga tgcggctgat aaggaattgg tcgctcgtta caaggaaacc
    56221 agacggagtc acccactagc agcagacggt cgtattttag atggaatcaa gttggaacgt
    56281 gaactcttgg cacctttgaa aaatatgagc caaaatgtgg tggatacgac tgaactcact
    56341 ccacgtgagc tgcgcaaaac ccttgcagag cagttttcag accaagaaca agcccagtct
    56401 ttccgtatcg aagtcatgtc tttcggattt aagtatggaa tcccgattga tgcggacttg
    56461 gtctttgatg tccgtttctt gccaaatccc tattatctac cagaactgag aaaccaaacg
    56521 ggtgtggatg aacctgttta tgattatgtc atgaaccatc ctgagtcaga agacttttat
    56581 caacatttat tggccttgat tgagccgatt ctgccaagtt accaaaagga aggtaagtcc
    56641 gttttgacca ttgccatggg atgtacgggt ggacaacacc gtagtgtggc atttgctaaa
    56701 cgcttggcgc aggacttatc caagaattgg tctgttaatg aagggcatcg cgacaaagac
    56761 cgcagaaagg aaacggtaaa ccgttcatga gaaaaccaaa gataacggtg attggtggag
    56821 ggactggaat tcccgtcatt ctaaaaagtc tgcgggaaaa agatgtggaa atcgcagcta
    56881 tcgtgacggt ggcagatgat ggtggttctt caggtgaact ccgaaaaaat atgcaacagt
    56941 tgacaccgcc aggtgatctt cgtaatgtcc ttgtggccat gtcggatatg cctaagtttt
    57001 atgagaaggt ctttcagtat cggttctctg aggatgccgg agcctttgct ggccatccat
    57061 tgggaaatct catcattgct ggcttgtcag aaatgcaggg ttcaacctat aatgccatgc
    57121 agttattgag caaatttttc catacaacag ggaaaattta tccttccagt gaccatcctt
    57181 tgacccttca tgcagtcttt caggatggga cagaagtggc tggagagagt catattgtag
    57241 accatcgagg cataattgac aatgtctatg tgaccaatgc cctaaacgat gatacgcctc
    57301 tggccagccg tcgagtagtg cagaccatcc ttgaaagtga catgattgtc ctagggccag
    57361 gttccctctt tacctctatt ttgcccaata tcgtgattaa ggaaattggg cgggctcttt
    57421 tggaaaccaa ggcagaaatt gcctatgtct gcaatatcat gacccaacgt ggggagacgg
    57481 aacactttac agatagcgac cacgtggaag tcttgcatcg tcaccttggt cgccctttta
    57541 tcgacactgt cttggtgaat attgaaaaag tgcctcagga atacatgaat tccaaccgtt
    57601 ttgatgaata cttggtgcaa gtggaacacg attttgtagg tctttgtaag caagtttcgc
    57661 gcgtgatttc atctaacttc cttcgtctgg aaaatggcgg tgccttccac gatggagatt
    57721 tgattgtgga cgagttgatg cgcattatac aggtgaaaaa atgagtttca cagtagcagt
    57781 aaaagaagaa atcctgggcc aacaccatct gagccggcat gaattatctg ccattatcaa
    57841 aatgtctggt agcatcggtc tctcgacttc gggcttgact ttgtcagttg tgacagaaaa
    57901 tgccaaactg gcccgtcacc tctatgagtc ctttctccat ttctatgaaa tcaaatctga
    57961 aattcgtcac caccaacgga gcaatcttcg caagaatcgc gtctataccg tttttacaga
    58021 tgaaaaggtg caggatttgt taagtgattt gcacttggca gactctttct ttggcctgga
    58081 aacaggtatt gatgaggcga ttttatcgga tgaggaagca ggtcgtgcct atctctgtgg
    58141 cgctttcttg gcaaatggga gcattcgtga ccctgagtca ggcaagtacc agttggaaat
    58201 cagttctgtt tatctggacc acgcgcaagg aattgcctcc cttctccaac agtttttact
    58261 ggatgccaag gtgcttgagc gcaagaaggg ggctgtgacc tatctccagc gagcagaaga
    58321 cattatggac ttcttgatag taatcggagc tatgcaggca cgtgatgatt ttgagcgggt
    58381 taagattttg cgagaaaccc gtaacgacct caatcgggct aataatgccg agacagctaa
    58441 tatcgctcgg acagtttctg ccagcatgaa gaccattaac aatatcagta aaataaaaga
    58501 tatcatgggc ttagaaaatt tgccagtaga tttgcaggaa gtggcgcaac tgaggattca
    58561 gcacccagac tactctatcc aacagttggc agatagcctc agcacccctc tgaccaaaag
    58621 tggtgtcaat cacagactca gaaaaatcaa taagatagcg gatgaattat aaaaccacga
    58681 attctatgtg actcgtggtt cttttttata aactagtaga gtgttttggt tgtacttttt
    58741 gttcagggtc aatgtagttg atagcgttgt tgacagcagt tggagcttct ccgaggcctg
    58801 tcgcaatcag atcaattttt ccgtcatagt agcagcagtc accgatagca tagatacctg
    58861 cttggctgga ttcctgtttg ctgttgacga taatcttgtg acggttgagg tcgagccccc
    58921 agtttttaag gttaccgaca gaagatttga aaccatagtt gacaaagagg tggtctaggt
    58981 caatggtttc agtttcatca gatttgactt ttgtgatttc aagtttatca agtgtttttc
    59041 catttccaag gagttggcta ggggcgaatg gtgtcttgat ggttacagat gattcttgca
    59101 aggcttgaac actgtgttcc aaggcacgga aattatctct gcggtgaaca agggtagttg
    59161 gtgcgatttt ttcaaaagcc aaagcccaat ccacagccga gtctccccca ccaagaatcg
    59221 tcactttctt accagcgtat tgctgaatgt tagaaacgtg gtagtggata ttttcatagc
    59281 cctcaacccc ttcaagttcc agcggacgtg gtttgaaggc accgccaccc atagcgatga
    59341 taactgtttt agtcaggtga cttcctttag aagttgtgat ggcaaattct tcttcttgtt
    59401 tgtcaatctc aagaaccgtt tcattgagat gaataggggt atcaaatcca tttagctgtt
    59461 caatcaagcg gttagtcaac tcttctccag tcaggtttgg gaagcctggt acgtctagga
    59521 tttccttttc agggtagaga atagcaggtt gtccacctag ctggggaaga gagtcgatga
    59581 tttgaacctt ggcttggcgt aggtgggcat aaaaggctgc aaaaagcccg acaggaccac
    59641 cacccacaat ggtaatatca tagagttgag acatagtttc tcctttgttt tttctagtca
    59701 gtttatttta tcatattttt gttcaatgta aaatggacta ggatagggaa aaaaatgcca
    59761 gaaaatggta taataggacg aaagtgtttt gatagggagg atatcaggga tgaattttca
    59821 acaattatcc aatctgcaat attggaccag tttgtttgca agtccatgga cgatagctat
    59881 caatctgatt gatattttga ttgttgctta tattttatac cattttacaa aagctattgc
    59941 aggaaccaag attatgattt tggtacgtgg agttttggtg tttattttag ctcaaatcct
    60001 tgcaaatatg attggtttga ctacgatttc ttggttaatc aatcaaatta ttacttatgg
    60061 ggttattgcg gcggttgtta tcttctctcc agagattcgg actggtttgg aacgtttggg
    60121 aagagcgaca gatttttttt ccaatgcccc tattagtgct gaggaacaga tgattcgtgc
    60181 ctttgttaag tctgttgaat acatgagtcc tcgtaaaatc ggggccttgg ttgctattca
    60241 gcgtgtacgt accttgcagg agtatatttc gacaggaatt cccttggatg ctaagatttc
    60301 tgcagaactt ctcattaaca tttttattcc caacactccc ctacatgatg gtgcggtgat
    60361 tatcaaagaa gaacgtatcg ctgtgacgtc tgcctatctg cccttgacaa aaaacacagg
    60421 gatttccaag gaatttggga ccagacaccg ggcggctatc ggtttatcag aagtctcaga
    60481 tgccttgact tttgtcgtat cagaggaaac gggaggaatt tcgataacct ataatggaag
    60541 gtttaagcac aacctaacac ttgatgaatt tgaaacagaa ttacgtgaaa tcttacttcc
    60601 aaaagaggaa gtgggtctta gttttaaaga acgattgcta ggaggatgga aacatgaaaa
    60661 aaaatagttt atatatcata tcctcactct tttttgcttg tgtcttattt gtctatgcta
    60721 cggcgacgaa ttttcaaaac agtaccagtg ctaggcaggt aaaaacggaa acctatacta
    60781 atacagtaac aaatgtccct attgacatac gctataatag tgataagtat tttattagcg
    60841 gttttgcttc agaagtatca gtggtcttga ctggggcaaa tcgcctatcg ctagctagtg
    60901 aaatgcaaga aagtacacgt aaattcaagg ttactgctga cctaacagat gccggtgttg
    60961 gaacgattga agttcctttg agcattgaag atttacccaa tgggctgacc gctgtggcga
    61021 ctccgcaaaa aattacagtc aagattggta agaaggctca gaaggataag gtaaagattg
    61081 taccagagat tgaccctagt caaattgata gtcgggtaca aattgaaaat gtcatggtgt
    61141 cagataaaga agtgtctatt acgagtgacc aagagacatt ggatagaatt gataagatta
    61201 tcgctgtctt gccaactagc gaacgcataa caggtaatta cagtggttca gtacctttgc
    61261 aggcaatcga ccgcaatggt gttgtcttac cggcagttat cactccgttt gatacaataa
    61321 tgaaggtgac tacaaaacca gtagcaccaa gttcaagcac atcaaattca agtacaagca
    61381 gttcatcgga gacatcttcg tcaacgaaag caactagttc aaaaacgaat taaaaataga
    61441 aaaggatttt ataacaatgg gtaaatattt tgggactgat ggagtccgtg gagaagctaa
    61501 cctagaacta acaccagaat tagcctttaa actaggacgt tttggaggct atgttcttag
    61561 tcaacatgaa acggaagcgc cgaaagtctt tgtaggacgt gacacacgta tttcagggga
    61621 aatgctggaa tcggccttgg tggcaggtct cctttcagta gggattcacg tatacaaact
    61681 tggtgtcctt gcaacaccag cagtagctta cttggttgaa actgaaggag caagtgccgg
    61741 tgtcatgatt tctgctagcc acaacccagc ccttgataac ggaatcaagt tctttggcgg
    61801 tgatggcttc aaactagatg atgaaaaaga agcagaaatt gaagccttgc tagatgctga
    61861 ggaagacact cttcctcgtc caagtgcaga aggcttagga attttggtag attatccaga
    61921 aggcttgcgt aagtatgaag gataccttgt ttcaactgga actcctcttg atggaatgaa
    61981 ggttgccttg gatacagcta atggagcagc ttctaccagt gcccgtcaaa tctttgcaga
    62041 ccttggtgcc caattgacgg ttatcgggga aacaccagac ggtcttaaca tcaaccttaa
    62101 tgttggttca acacatccag aagcccttca agaagtggtc aaagaaagtg ggtcagctat
    62161 tggtttggcc tttgatggag acagtgaccg cttgattgct gttgatgaga atggtgacat
    62221 cgtcgatggt gacaagatta tgtacatcat cggaaaatac ctttctgaaa aaggacaatt
    62281 ggctcaaaat acaattgtga caactgttat gtctaacctt ggtttccaca aggccttgaa
    62341 tcgcgaaggt attaacaagg cagttactgc agttggtgac cgctacgttg ttgaagaaat
    62401 gagaaaatca ggctacaacc ttggtggtga acagtctggt cacgttatct tgatggatta
    62461 caataccaca ggtgatggtc aattatcagc agttcaattg actaaaatta tgaagaaaac
    62521 tggtaaaagc ttatcagagt tggcggcaga agtaacgatt tatccacaaa aattagttaa
    62581 tatccgagtg gaaaacgtca tgaaggaaaa ggccatggaa gtgccagcta tcaaggccat
    62641 catcgagaag atggaagaag aaatggcggg gaacggccgt atccttgttc gtccaagtgg
    62701 aacagagccc ctcttgcgtg ttatggcaga agcgcctaca acagaagaag tggactacta
    62761 tgttgatact atcacagatg tagttcgtgc tgaaattggg attgactaaa tcctagaaaa
    62821 ctagatgaaa ataaaaaatt atggtacaat agcttataat attgtagccg atggagaaag
    62881 acatgaatct taaacgtgaa caagaatttg ttagtcagta tcattttgat gcgcgtaatt
    62941 ttgaatggga aaatgaaaat ggagctcctg aaaccaaggt ggatgtgaat ttccaattgc
    63001 tccaacatga ccaagaaaat caagtgactt ccttgattgt catcttgagc tttatgattg
    63061 tatttgataa gtttgtcatt agtggaacaa tctcacaagt aaaccatatc gatggccgta
    63121 ttgtcaatga accaagtgaa ttgaaccaag aagaggtgga aactttggct cgtccatgtt
    63181 tgaacatgct caatcgtttg acttacgaag tgactgaaat tgcattagac ttaccaggaa
    63241 ttaatttgga gttctaatat gaaattagct gttttcacag attcctctgc ttatctcagt
    63301 gcagagacct tgcaaagaga agacttgttt gtcttggata ttcctgttaa tattgatggt
    63361 gaggaatatg tcgaaggcat caatctgtct gctgaggaat tttaccaaaa aatggctcag
    63421 gcttctgaat tgcctaagac cagtcaacca agtattgcca agttagatga aatcttaact
    63481 tcgctcaaag aacaaggcta tacacatgcc ttggggcttt tcctatcttc tggaatttca
    63541 ggtttttacc aaaatatcca gtatatggtc gatgactatg agggcttaac cattgctttc
    63601 ccagacactt tgattacaag tgctccccta ggcatcatgg ttgaaagcgt ctttaattgg
    63661 cgtgatcagg gcgatgattt tgccagcatt caggataagc tagccattca aatcagccgt
    63721 acgtcagcct ttatcatggt agatgacttg gatcatttgg taaaaggtgg acgcctttca
    63781 aatggggctg ccattttggg caatttgctt agcattaagc caatccttta ttttaacgat
    63841 caaggtgtga ttgaagttta cgaaaaagtt cgtactgaaa agaaggccac caagcgctta
    63901 attgaaatta tcaaggaaac aacggcttca ggtcaatacc gggtcattgt cattcatggg
    63961 aatgctcctg aaaaggctga agaattgcgt cagcacttgc ttgattttgg cttgggttcg
    64021 gatgtttcac ttgctacatt tggtagtgtc attggaacgc acctaggagc aggaagtatt
    64081 gctctgggtt atattccagt gatttagttg gcgcttctag gctagttaag atgccttgta
    64141 tcttagaaat tgaacacgga ctacctgcct cttgaaaaaa gatgcctgtc ttgcctttcg
    64201 tggaaagtca gtgccattcc ctatttttca gaggcagcta aggtcctttg tatcttgata
    64261 attgaacacg cctggaaccc tgtgtgaaaa agatagttct tccaagaagt agacacttct
    64321 tgattggaat tcctattttc actttgtgtt cttacggact ttgtatctta gacaggagta
    64381 gagatgagta ttcgagtaat tattgccggt tttaagggaa agatgggtca agctgcttgc
    64441 cagatggtct tgactgattc agatttggac ttggtagcag ttttggatcc ttttgagtct
    64501 gagtcagaat ggcagggtat tcctgttttc aaggataagg ctgatttagc tggttttgaa
    64561 gcggatgtct gggtagattt tactactcca gctgttgcct acgaaaatac acgttttgct
    64621 cttgaaaatg gctttgctcc agtagttgga acgactggtt tcacgagtga agaaattgca
    64681 gagttaaaag aattttctcg tgcccaagac ttgggtggcc tgattgcccc taactttgcc
    64741 ttgggtgctg tcttactcat gcaatttgcg acgcaggctg ccaaatattt cccaaatgtg
    64801 gagattattg agctccatca tgacaagaaa aaggatgctc cgagtggaac agccattaaa
    64861 acagctgagt tgatggcaga ggttcgagag tccattcagc aaggcgcagc agatgaggaa
    64921 gagctgattg ctggtgctcg tggtgctgac tttgatggta tgcgcatcca ctcagttcgt
    64981 ttgccaggtt tggtagccca tcaggaagtc atctttggca atcaaggaga aggattgacc
    65041 ctccgtcatg actcctatga tcgcatctcc ttcatgacag gagtcaattt gggaattaaa
    65101 gaagttgtca agcgtcatga gcttgtctat ggattagaac acttattatg agattaacgc
    65161 aaatgccttc tgaatttcag aaggctttac cagtattaga aaaaattaaa gaagcaggct
    65221 ttgaggctta ttttgttggg ggctctgttc gagatgccct tctccatagc cctatccacg
    65281 atgtggatat tgcgacgtct tcttatccag aagagaccaa gcagattttt ccgcgaacag
    65341 ccgatatcgg aatcgagcat ggaaccgtct tggtcttaga tggggatgag gagtatgagg
    65401 taacaacctt tcggacagag gatgtctatg tggactatcg cagacccagt gcggtttcct
    65461 ttgtgcgctc gctagaagaa gacctcaaac gccgtgattt cacagtcaac gcctttgcct
    65521 tggatgagac aggagaaatc gttgacttgt tccatggttt agaagatttg gaaaagcaag
    65581 tcttgcgagc agttggagtg gctagtgagc gtttcaacga agatgcttta cggattatgc
    65641 gtggtttccg ttttcaggct agtcttggtt ttgcacttga gccagaaaca tttaaagcta
    65701 tgaagacctt gacgccgctt ttggagaaca tttctgtaga gcgtaccttc gtcgagtttg
    65761 ataaactctt gctggctcca ttttggagaa ggggcttggc ttccatgatt gagagtcaag
    65821 cttatgacta tctccctgat atggcatcta gccagaacaa gctcaacaga ctgtttgatt
    65881 tggagactga ttttactttt gaatcctctg aacaagcctg ggcggcttta ctatgggctt
    65941 tggagattga aaatgcgcag tcatttttga aatcttggaa gacctcacgc cagtttgcca
    66001 agcaagttca ggatttgctg attattttgg ctctgcgtga aaatggagaa ttgagcaagc
    66061 gagattgtta tcgctttgac atagatttgc ttttacaggc tgaaaatctt cgtcaagctc
    66121 gaggaaaaga agtcaaccca caagccatca cagaaaaata ccaaagcttg actatccatg
    66181 acaagaaaga gattcagatt aatggcgata ttttgatcaa ggaatatggc tatcagccag
    66241 gcccagactt gggagagatt ttaacagaga ttgagtttgc cattgtcgat ggagaattgg
    66301 agaataatcg tgaagccatc catgcttacc tgagggagaa aaaatgagtg attttatcgt
    66361 tgaaaaacta agtaaatccg ttggtgacaa gaccgttttt agggatattt cctttattat
    66421 ccatgactta gacagaattg gtttaatcgg tgtcaatggg actggcaaga ccaccctttt
    66481 ggacgtcctt tctggtgttt ctggctttga tggggatgtc agtccttttt cagctaaaaa
    66541 tgattaccag attggttact tgactcagga tcctgatttt gatgatagaa agacagtttt
    66601 ggatacggtt ctatctagtg aactcaagga aatccagctc attcgtgagt atgaattgat
    66661 tatgctcgac tatagtgagg acaagcaggc gcgtttggaa cgtgtcatgg cagagatgga
    66721 ctctctccaa gcttgggaaa tcgagagtca ggtcaagacc gttcttagca aattgggcat
    66781 tcaagactta tctactcctg ttggggaatt gtcaggtggt ctgagaagac gggtacagtt
    66841 ggcacaagtc ttacttggca accacgacct cttgcttttg gatgagccga ccaaccatct
    66901 ggatattgcg attattgagt ggctgaccct ctttttgaaa aattctaaga agaccgtcct
    66961 ttttatcact cacgatcgtt atttcttaga cgctttgtca acacggattt tcgagttgga
    67021 gcgagcaggc ttgacagaat atcagggcaa ttaccaggac tatgttcgcc ttaaggcaga
    67081 acaggacgag cgcgatgcaa ctctacttca taaaaaagaa caactctaca aacaagaact
    67141 ggcctggatg cgcagacaac cgcaggcacg tgcgaccaag caacaagctc gtatcaatcg
    67201 tttccatgat ctgaaaaagg aagtttcagg cagtagtgct gagacagact tgactatgaa
    67261 ctttgaaacc agtcggattg ggaagaaagt catcgagttt caggatgttt cctttgccta
    67321 tgaaaataag cccattttgc aagattttaa tctcttggta caggtcaaag atcgtatcgg
    67381 aatcgttggg gacaatggtg ttggaaaatc aaccctactt aacctgattg caggaagtct
    67441 tgagccgata gcaggacaag ttgtgattgg ggaaactgtt cgcatcgcct atttctctca
    67501 acaaattgag ggtttggatg aaagcaagcg tgtgatcaat tacctgcagg aagtggcaga
    67561 ggaggtcaag accagtggtg gttctacgac ttccatcgct gagttgctgg agcaattcct
    67621 cttcccacgt tcgacgcatg ggaccttgat tgagaaatta tctggtggag agaaaaaacg
    67681 tctttatctc ctcaaactgc tcttggagaa accaaatgtt cttcttttag acgagccaac
    67741 caatgacttg gatattgcaa ctttgacagt cttagagaat ttcttgcaag gttttgcagg
    67801 tcccgtttta acagtcagtc acgaccgcta tttcttggat aaggtagcga ccaagattct
    67861 cgcttttgag gatggcaaga ttcgtccttt ctttggtcat tacaccgact atcttgatga
    67921 aaaagctttt gaaacagata tggccaatca agtgcaaaag gccgaaaagg aaaaagtggt
    67981 caaggttcga gaagacaaga aacgcatgac ctaccaagaa aagcaggagt gggcaagtat
    68041 tgaaggtgat attgaaacct tggaaaaacg tatcgctgct attgaagagg aaatgcaggc
    68101 taacggctct gactttggta agctggctac tctccaaaaa gaattggatg agaaaaatga
    68161 agcactcctt gaaaaatacg aacgctatga gtatctcagt gaatttgata gttaagacta
    68221 ttcgaaaatc aaagtgcaaa ctatgtcggc ttcacattgc cgactcaagt acagcctgtg
    68281 accggcttcc taatttgcac tttgattttt attgagtata aaagaataga aaaatcccgt
    68341 ctcatgaaag gatttctcta ttcttttttg gtttcttgat gaaagatatt ttgccaggtt
    68401 tcgtggcgac gccagatact ggtatggatg ataccatcta actcatagca gatgagtttg
    68461 gttttttgac tgatggaagt aatttgaatg tccttgatag cagaatttaa ctcttttttg
    68521 gtcttataag cctctttatc catctgctct ccatcttggc gaatatagac aaaatcatca
    68581 gtcaagagtt cttctagttg gtttccttgg tcaatcaatt gttcacgcat aagcaatttt
    68641 ttatagacat tgtctaaaat ttcatcctca gggataggtg ctggttcgat atggacatcg
    68701 gtatcaaaga cgccaaaacg ctcctccagc atagactcga cctgatccgc gatttcatgg
    68761 ctttcaaaaa cagacaagtc aggattcatc tctagtgtaa tatccaggta gatgttgcta
    68821 ccgtaggtgc gacctctttg cgatttgacc ttgctgattt tgggaatttc catgatagcc
    68881 ttttggtagt cctcgagcag gcggtcgtca aagccatctg aaagactaaa ggaagactcg
    68941 atgaagatat cataggcagt cttcaagata aagaaagtga tgatgatagc aaccagttta
    69001 tccacaatcg gataattgaa actactagct aggatggcaa tggcggttcc aagtgaggta
    69061 acagcgtcag aaagattgtc cttagcagct gccttcagcg ccttggagtt ggatttctta
    69121 ctgaggcgag tattgtagag atagaccaca aacataatcg ctgcagaaat gattcctaga
    69181 gttgcaccaa gaggatcaat gaccgtttct tcccgactga gaatcttttg aatggtatct
    69241 cttagaacat cgaaaccgac atagaacatg atgatagaag tgatcaagct tgccaaatct
    69301 tcaatcttcc aatgaccaaa acggtggtca cggtctgcag gctggcgcgc catccgaatc
    69361 ccgattaaga gggccacatt tccaatgatg tccgatacgt tattaaaacc atcggccacc
    69421 aaactggatg aatgaaggag atgaccagct gctaatttag ctgcagacaa gatcaaatag
    69481 gtcgaaatac tgataatggc tccacgctca gctaacttga gatttgagat agattgcttc
    69541 attcaacttc ctttctagtc acatagcatt ctaccattat actggttttc aaggaaaaat
    69601 tcaaataagg cagtttttac tctttgaaaa ctccccaaaa tttggtataa tggtactact
    69661 caagggagta gctggcagaa acctgtgata gtgtcgtcat tccgaatttt atgctgaaaa
    69721 gtatgctttc cggccctatc ttaaacagcg agacttgtta tgattaacag gtctcttttt
    69781 gtttgtcgtg aaaagatatg acagaaagag agtctgtttt gcacacaatg tcaaggagga
    69841 gacacatgtc aaaagaacaa aaacgccaag cgttttatac tcagagccct gaagaggtct
    69901 tgcaggctgt ggatgcgacc gagcaaggtt tgtcatcaag tgaggcggaa aagcgccttg
    69961 ccgaatttgg gcacaatgaa ctcgaagaag gcgagaaacg atcaatcttg gtcaaattca
    70021 tcgagcaatt taaggatttg atgattatca tcctagttgc ggcagcaatc ttgtcagtcg
    70081 tgacttctgg tggggaagat atcgcagatg ccattatcat cctagctgtg gttatcatca
    70141 acgctgcctt tggtgtttac caagaaggaa aagctgaaga agctatcgaa gccctcaaat
    70201 ccatgtctag tccagttgcc cgcgttcttc gtgatggaca catggcagag attgactcta
    70261 aagaattggt accaggcgat atcgttgccc ttgaagcagg tgacgtggta ccagcggacc
    70321 tacgtttgct agaagccaac tctcttaaaa ttgaagaagc agccttgacg ggtgaatctg
    70381 taccagtcga aaaagacttg tcagtcgagc ttgcgacaga tgctggtatt ggtgaccgtg
    70441 tcaacatggc cttccaaaac tcaaacgtaa cctatggtcg tgggatgggt gttgttgtca
    70501 atacaggtat gtacactgaa gttggtcata ttgctggtat gcttcaagat gcggatgaga
    70561 ctgatacacc actcaaacaa aacttgaaca acctttctaa ggtcttgacc tatgctatct
    70621 tggtcattgc ccttattact tttgtagtgg gtgtcttcat tcaagggaaa aatccacttg
    70681 gtgagttgtt gacttctgtt gcccttgccg ttgcagccat tccagaagga cttcctgcta
    70741 tcgttaccat cgttctttct cttggtactc aagttttggc caaacgacat tccatcgttc
    70801 gtaagttgcc agcagttgaa acacttggtt caactgaaat catcgcttct gataagactg
    70861 gtacgctgac tatgaacaag atgacagtcg aaaaagtctt ctacgatgcg gttctacatg
    70921 actcagctga tgatattgaa ctaggtcttg aaatgccact acttcgttca gttgtcttgg
    70981 ccaatgatac gaaaatcgat gtggaaggta acttgattgg tgacccaacc gaaacagcct
    71041 ttatccaata tgccttggac aagggctatg atgtcaaagg tttcttagag aaatatcctc
    71101 gtgtagctga attgccattt gactctgacc gtaagctcat gtcaacagtt cacccattgc
    71161 cagatggtcg tttccttgta gcagtcaagg gtgcgccaga ccaactctta aaacgttgtc
    71221 ttcttcgtga taaggctggg gatattgctc cgattgatga gaaggttaca aatctcattc
    71281 atacaaacaa ttctgaaatg gctcatcaag ccttgcgtgt ccttgcaggt gcttataaga
    71341 ttatcgatag tattccagaa aatctcactt ctgaagagct tgaaaatgat ttaattttta
    71401 ctggtttgat tgggatgatt gaccctgaac gtcctgaagc cgctgaggct gttcgtgtgg
    71461 ctaaggaagc gggaatccgt ccgattatga ttacaggtga ccaccaagac acagcggaag
    71521 ccattgccaa acgtttggga atcattgacg caaacgatac agaaggtcac gttttaactg
    71581 gtgctgaact caatgaactg tcagatgaag aatttgaaaa agtcgttggt caatactctg
    71641 tttatgcccg tgtgtctcca gaacacaagg ttcgtatcgt caaggcttgg caaaaacaag
    71701 gtaaagtcgt tgccatgaca ggtgacggtg tcaatgacgc gccagctctg aaaacagccg
    71761 atatcggtat cggtatggga atcactggta cagaggtttc taagggggct tctgatatga
    71821 ttcttgcaga tgataacttt gcgactatta tcgtcgcagt ggaagaagga cgtaaggtct
    71881 tctcaaacat tcaaaagact attcagtacc tactttctgc taatactgct gaagtattaa
    71941 ccatcttcct atcaaccttg tttggttggg atgtcttaca gccggttcat cttttgtgga
    72001 tcaacttggt aacggatacc ttcccagcta tcgctcttgg tgttgaacct gcggaacctg
    72061 gtgtcatgaa tcataaacca cgtggacgca aggcaagctt cttctcaggt ggtgttttga
    72121 gttctatcat ttatcaaggt gtactccaag cagctcttgt tatgagtgtt tatggccttg
    72181 cgattgctta cccagttcat gtgggtgaca atcatgctat tcatgcagat gccctaacga
    72241 tggcctttgc aacccttggt ttgattcagc tcttccatgc ctacaatgtc aagtctgtct
    72301 accaatccat cttgacagtt ggcccattca agtctaagac ctttaactgg tccatcttgg
    72361 tatcctttat ccttctcatg gcaacaatcg ttgtagaacc gcttgaagga atcttccacg
    72421 taaccaaact agacttgtca caatggggaa ttgttatggc tggaagcttc tcaatgatta
    72481 tcatcgtcga aatcgttaag tttattcaac gcaaactcgg ttttgacaag aatgcgattt
    72541 aagggaggtt tcggatgtca gcatatcaat taccgaccgt atggcaggat gaagctagta
    72601 atcaaggagc ttttacgggg ctaaacagac caacagcagg tgcccgtttc gaacaaaact
    72661 tgccaaaagg agaacaagct tttcagcttt attcactggg aacaccaaat ggtgtgaagg
    72721 ttactatctt attggaagaa ttactagaag ctggttttaa ggaagcggct tacgacttgt
    72781 ataagattgc tatcatggat ggggatcaat tcggctcaga ctttgtgaag ctcaatccaa
    72841 attccaagat tccagcctta ttggaccagt caggtactga aaacgtaaga gtctttgagt
    72901 ctgctcatat tcttctttac cttgctgaga aatttggagc ctttttacca agtaatcctg
    72961 tggaaaaggt agaagttttg aattggctat tctggcaagc aggtgcagca ccttttctag
    73021 gtgggggatt tggacatttc ttcaattatg ctcctgaaaa attggaatat cctattaacc
    73081 gttttacgat ggaagtgaaa cgccagttgg atttattgga taaggaattg gctcagaaac
    73141 cttatattgc aggcaatgac tatacgattg cagatattgc tatctggtct tggtatggac
    73201 agttagttca aggaaatctt taccaaggtt ctgcaaaatt tttggatgcc tcaagttatc
    73261 aaaatctagt aaaatgggca gaaaaaattg ccaatcgtcc agctgttaag cgtggattgg
    73321 aagtaactta tacagaaatt aaatagcaaa aaagtcatct tcggatggct ttttgctaca
    73381 gttgagggaa aggttttgca gttatcggct tttgtgctat aattgctata atatagtaaa
    73441 aagcaagagg agtgtgagga agtggaaaag aaaattgtga aggatatctt atttttatct
    73501 caagtgtctc agccggcaag tcaggaggac ctttatcttg ccagagattt gcaggataca
    73561 ctcttagcaa atcgtgatac ctgtgttggt ctagctgcca atatgattgg ggtgcagaag
    73621 cgcgtgatta tctttaatct tggcttggtt cccgtggtca tgtttaaccc agtgcttctg
    73681 tcctttgaag gatcttatga ggcagaagaa ggctgtttgt ccttggtagg tgtgagatca
    73741 actaagcgtt atgaaaccat aaggcttgcc tatcgtgaca gcaagtggca ggaacagacc
    73801 attaccttga caggcttccc agctcagatt tgccagcatg agctggatca cttggaagga
    73861 cgaatcattt aggaggaaag caaatgaaac gaatagtctt tgaacttatt tttatcgcaa
    73921 cgacctggta tatcttttta ccgcccctta acctgaccag ctgggaattt ctcttcttcc
    73981 tctgtgggca tttgttagtt gtggcaatct tatttggctt tggcaagggg ataaaccttg
    74041 tcaaaacggt tcatgtgcgc cacggtaagg cggaagctgc cttaaatctt gagggtttca
    74101 aaatcaatcg gttagggaaa attctgttag cttcgattgg aggaattctt ctcttggcag
    74161 ctttggtttc cttggtaact tccagcatgt ttcaggctaa aaattatgcc aatgtagtca
    74221 cggttacgga aaaagacttt actgaatttc ctaggagtga caccagtaag gttcctatcc
    74281 tagatagaag tactgctgaa aaaattggag accgctactt gggttcccta accgataagg
    74341 tgtcgcaata cgtagcggca gatacctata cccaattgac aattgatggg aaaccttatc
    74401 gggtcacacc actagaatat gcagacccta tcaaatggtt taacaatcaa gccaagggaa
    74461 tcggtgagta tatcaaggtg gacatggtaa ctggaaatgc ggatttggtg gacttgaaga
    74521 caccaatcaa gtattcagac tcggagtatt ttaaccgtga tgtcaaacgt cacctgcgct
    74581 tgaagtaccc gactaaaatc tttaaaactc catcttttga ggtggacgat gagggcaatc
    74641 ctttctatgt agcaacggtt taccaaaagc aatttggact tgcggttcct cgtcctgctt
    74701 cagtcattat cttggatgct acaaatggag aaaccaagga atacagctta tcagatgttc
    74761 cagaatgggt ggacagggtc tatccagcag aggaaaccat tgagcaaatc aactacaacg
    74821 gcaagtacaa ggacggtttc ttgaatgcca tgatttccaa gaaaaatgtg acccagacta
    74881 ccaagggcta taattacttg tctatcggta atgacatcta tctctacaca ggtgtgacgt
    74941 cggctaatgc ggatgagagt aatcttggtt tcatccttga aaatatgcga acaggagaaa
    75001 tcactaagta tagcttggct tctgcgacag aagaatcagc ccgtgaatca gcagaaggtg
    75061 ctgttcagga gaaatcctac aaagcaacct tcccaatcct catcaacctc aatgacaagc
    75121 ctctctacat catgggctta aaggacaatg ctggcttggt caaagagtac gtcctggtag
    75181 acgcagtcga gtaccaaaat gttatcgttg ctactacagt ggaagagatg ctcagcaagt
    75241 atgccaataa aaacgacctt gaaattgaca atgcaacgac agaaagcatc aagggagtag
    75301 tagcagacct caaatcagct gttatcaagg gagacactgt ctacttcttt aaagttgatg
    75361 gcaagatata caaggtcaag gcttcagtat ccgatgacct tccttacctt gaaaatggta
    75421 aaaccttcga aggtcaagta ggaaaagaca attatctcaa gacctttaaa gtacaataat
    75481 actcttcgaa aatctctagc tctatctgca acctcaaaac agtgttttga gcagcc
//
LOCUS       DAVKJD010000003        70118 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKJD010000003 DAVKJD010000000
VERSION     DAVKJD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 70118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70118
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.003"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            2..74
                     /locus_tag="VW157_000163"
     tRNA            2..74
                     /locus_tag="VW157_000163"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            99..171
                     /locus_tag="VW157_000164"
     tRNA            99..171
                     /locus_tag="VW157_000164"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Lys,seq:ttt)
     gene            177..258
                     /locus_tag="VW157_000165"
     tRNA            177..258
                     /locus_tag="VW157_000165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Leu,seq:tag)
     gene            277..349
                     /locus_tag="VW157_000166"
     tRNA            277..349
                     /locus_tag="VW157_000166"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Thr,seq:tgt)
     gene            372..443
                     /locus_tag="VW157_000167"
     tRNA            372..443
                     /locus_tag="VW157_000167"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Gly,seq:gcc)
     gene            451..536
                     /locus_tag="VW157_000168"
     tRNA            451..536
                     /locus_tag="VW157_000168"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:taa)
     gene            546..619
                     /locus_tag="VW157_000169"
     tRNA            546..619
                     /locus_tag="VW157_000169"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:580..582,aa:Arg,seq:acg)
     gene            635..708
                     /locus_tag="VW157_000170"
     tRNA            635..708
                     /locus_tag="VW157_000170"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:669..671,aa:Pro,seq:tgg)
     gene            714..787
                     /locus_tag="VW157_000171"
     tRNA            714..787
                     /locus_tag="VW157_000171"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Met,seq:cat)
     gene            801..874
                     /locus_tag="VW157_000172"
     tRNA            801..874
                     /locus_tag="VW157_000172"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Ile,seq:cat)
     gene            885..974
                     /locus_tag="VW157_000173"
     tRNA            885..974
                     /locus_tag="VW157_000173"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Ser,seq:tga)
     gene            988..1061
                     /locus_tag="VW157_000174"
     tRNA            988..1061
                     /locus_tag="VW157_000174"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1022..1024,aa:Met,seq:cat)
     gene            1065..1137
                     /locus_tag="VW157_000175"
     tRNA            1065..1137
                     /locus_tag="VW157_000175"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1098..1100,aa:Phe,seq:gaa)
     gene            1157..1227
                     /locus_tag="VW157_000176"
     tRNA            1157..1227
                     /locus_tag="VW157_000176"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1189..1191,aa:Gly,seq:tcc)
     gene            1263..1336
                     /locus_tag="VW157_000177"
     tRNA            1263..1336
                     /locus_tag="VW157_000177"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Ile,seq:gat)
     gene            1343..1430
                     /locus_tag="VW157_000178"
     tRNA            1343..1430
                     /locus_tag="VW157_000178"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1377..1379,aa:Ser,seq:gct)
     gene            complement(1556..2491)
                     /locus_tag="VW157_000179"
     CDS             complement(1556..2491)
                     /locus_tag="VW157_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9551550.1"
                     /translation="MKSYQAVYQILAKETDYISGEKIAEELSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(2488..3348)
                     /locus_tag="VW157_000180"
     CDS             complement(2488..3348)
                     /locus_tag="VW157_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9551551.1"
                     /translation="MLVFSEYQTGTIDLSLSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGTFHYKGKIVDLKEGDFFLLKPEELTFYQADSEEPWSYYWLGITGVKAPDYFALS
                     QIFDQAYLIQSETCHTQSTAKLISDIVRFAQITKSNELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTYQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRAKQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEHS
                     KYQLVRKETL"
     gene            3456..5618
                     /locus_tag="VW157_000181"
     CDS             3456..5618
                     /locus_tag="VW157_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9551552.1"
                     /translation="MGVRIENNLFYVESKNLSLIVENRDGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTSFDNDSTIASYSKLE
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSIGSNRGAS
                     GHAQTPALLLCDQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRLAMGINPENFSW
                     KLDPEENFETPVALVTFSDKGLTGVSHESQNFVLKHILPSKFSTKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWSVNEKKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRKHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRTTPLETRGHVAMMGNLGYELDLTSLSDEEKAEIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYKNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     ELQENGEVYSGAELMYAGLTVILSQGDFLSKQYIFRRL"
     gene            5653..6912
                     /locus_tag="VW157_000182"
     CDS             5653..6912
                     /locus_tag="VW157_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9551553.1"
                     /translation="MKWYKKIGLLATTGLALVGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            6926..7792
                     /locus_tag="VW157_000183"
     CDS             6926..7792
                     /locus_tag="VW157_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9551554.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            7806..8639
                     /locus_tag="VW157_000184"
     CDS             7806..8639
                     /locus_tag="VW157_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9551555.1"
                     /translation="MKQDERKALIGKYILLILGSVLLLVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            8950..10392
                     /gene="gtfA"
                     /locus_tag="VW157_000185"
     CDS             8950..10392
                     /gene="gtfA"
                     /locus_tag="VW157_000185"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9551556.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFRDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINCHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            10530..11723
                     /locus_tag="VW157_000186"
     CDS             10530..11723
                     /locus_tag="VW157_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9551557.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLGYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            12410..14389
                     /locus_tag="VW157_000187"
     CDS             12410..14389
                     /locus_tag="VW157_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9551558.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            14456..15952
                     /locus_tag="VW157_000188"
     CDS             14456..15952
                     /locus_tag="VW157_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9551559.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            15952..16878
                     /locus_tag="VW157_000189"
     CDS             15952..16878
                     /locus_tag="VW157_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9551560.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            16887..17954
                     /locus_tag="VW157_000190"
     CDS             16887..17954
                     /locus_tag="VW157_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9551561.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            17965..18891
                     /locus_tag="VW157_000191"
     CDS             17965..18891
                     /locus_tag="VW157_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9551562.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAPELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            19340..20317
                     /locus_tag="VW157_000192"
                     /pseudo
     CDS             19340..20317
                     /locus_tag="VW157_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260583.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            20329..20769
                     /locus_tag="VW157_000193"
     CDS             20329..20769
                     /locus_tag="VW157_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9551563.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGVYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLAILLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVLNLSLGDSATVFKQLASLGLTNEGAVFSLSLVCLLLVCS"
     gene            20778..21206
                     /locus_tag="VW157_000194"
     CDS             20778..21206
                     /locus_tag="VW157_000194"
                     /inference="COORDINATES: protein motif:HMM:NF014684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551564.1"
                     /translation="MKTEIGLVLRSTGDNIPISEANGVNVDTMKIVGYMISNGLIALC
                     GSLFAQNDGFSDVTSGTGTIVVGLSSEVLIHDLIIGGRLLSIGIGAIVYRLIILNIYE
                     IPNLDQNLVRLFNAILLALVLFAPELQKRLKIRGLKLRNE"
     gene            21219..22022
                     /locus_tag="VW157_000195"
     CDS             21219..22022
                     /locus_tag="VW157_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9551565.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFIYVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQYPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     DNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(22054..22764)
                     /gene="treR"
                     /locus_tag="VW157_000196"
     CDS             complement(22054..22764)
                     /gene="treR"
                     /locus_tag="VW157_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9551566.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            22949..24916
                     /gene="treP"
                     /locus_tag="VW157_000197"
     CDS             22949..24916
                     /gene="treP"
                     /locus_tag="VW157_000197"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9551567.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVIAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            25094..26719
                     /gene="treC"
                     /locus_tag="VW157_000198"
     CDS             25094..26719
                     /gene="treC"
                     /locus_tag="VW157_000198"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9551568.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWTPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVGIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            26975..27223
                     /locus_tag="VW157_000199"
     CDS             26975..27223
                     /locus_tag="VW157_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9551569.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            27430..28224
                     /gene="racE"
                     /locus_tag="VW157_000200"
     CDS             27430..28224
                     /gene="racE"
                     /locus_tag="VW157_000200"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9551570.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            28221..29192
                     /locus_tag="VW157_000201"
     CDS             28221..29192
                     /locus_tag="VW157_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9551571.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            29168..29689
                     /locus_tag="VW157_000202"
     CDS             29168..29689
                     /locus_tag="VW157_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9551572.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            29686..30147
                     /locus_tag="VW157_000203"
     CDS             29686..30147
                     /locus_tag="VW157_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9551573.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYDIRCQ"
     gene            30138..30878
                     /gene="xerD"
                     /locus_tag="VW157_000204"
     CDS             30138..30878
                     /gene="xerD"
                     /locus_tag="VW157_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9551574.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            30878..31606
                     /locus_tag="VW157_000205"
     CDS             30878..31606
                     /locus_tag="VW157_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9551575.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            31625..32194
                     /locus_tag="VW157_000206"
     CDS             31625..32194
                     /locus_tag="VW157_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9551576.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            32181..32903
                     /locus_tag="VW157_000207"
     CDS             32181..32903
                     /locus_tag="VW157_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9551577.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            32903..33145
                     /gene="yidD"
                     /locus_tag="VW157_000208"
     CDS             32903..33145
                     /gene="yidD"
                     /locus_tag="VW157_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9551578.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVSDHFSLKRNQEGE"
     gene            complement(33370..34335)
                     /locus_tag="VW157_000209"
     CDS             complement(33370..34335)
                     /locus_tag="VW157_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9551579.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEITI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(34431..35183)
                     /locus_tag="VW157_000210"
     CDS             complement(34431..35183)
                     /locus_tag="VW157_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9551580.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(35180..36136)
                     /locus_tag="VW157_000211"
     CDS             complement(35180..36136)
                     /locus_tag="VW157_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9551581.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSFFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQVLIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            complement(36126..37085)
                     /locus_tag="VW157_000212"
     CDS             complement(36126..37085)
                     /locus_tag="VW157_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9551582.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLTLFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRSYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(37508..38209)
                     /gene="galE"
                     /locus_tag="VW157_000213"
     CDS             complement(37508..38209)
                     /gene="galE"
                     /locus_tag="VW157_000213"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9551583.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDTSNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            38610..39674
                     /gene="pepA"
                     /locus_tag="VW157_000214"
     CDS             38610..39674
                     /gene="pepA"
                     /locus_tag="VW157_000214"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9551584.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            39696..39992
                     /locus_tag="VW157_000215"
     CDS             39696..39992
                     /locus_tag="VW157_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9551585.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            40678..41121
                     /locus_tag="VW157_000216"
     CDS             40678..41121
                     /locus_tag="VW157_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9551586.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            41125..41889
                     /locus_tag="VW157_000217"
     CDS             41125..41889
                     /locus_tag="VW157_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9551587.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTETFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVIPKVEDLTNRGKF"
     gene            41904..42632
                     /locus_tag="VW157_000218"
     CDS             41904..42632
                     /locus_tag="VW157_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9551588.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            42625..44145
                     /locus_tag="VW157_000219"
     CDS             42625..44145
                     /locus_tag="VW157_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9551589.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATIKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            44490..45296
                     /locus_tag="VW157_000220"
     CDS             44490..45296
                     /locus_tag="VW157_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9551590.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDCIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(45463..45999)
                     /locus_tag="VW157_000221"
     CDS             complement(45463..45999)
                     /locus_tag="VW157_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9551591.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHFVVTNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            46132..47022
                     /locus_tag="VW157_000222"
     CDS             46132..47022
                     /locus_tag="VW157_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9551592.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     VSLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            47266..47619
                     /locus_tag="VW157_000223"
     CDS             47266..47619
                     /locus_tag="VW157_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9551593.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            47620..48657
                     /locus_tag="VW157_000224"
     CDS             47620..48657
                     /locus_tag="VW157_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9551594.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(48703..49710)
                     /gene="galR"
                     /locus_tag="VW157_000225"
     CDS             complement(48703..49710)
                     /gene="galR"
                     /locus_tag="VW157_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9551595.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRENTLNQ
                     E"
     gene            49874..51052
                     /locus_tag="VW157_000226"
     CDS             49874..51052
                     /locus_tag="VW157_000226"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9551596.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYKVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            51071..52552
                     /locus_tag="VW157_000227"
     CDS             51071..52552
                     /locus_tag="VW157_000227"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9551597.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEETVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDETPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            52622..53026
                     /locus_tag="VW157_000228"
     CDS             52622..53026
                     /locus_tag="VW157_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9551598.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEDRQVRI"
     gene            53183..54037
                     /locus_tag="VW157_000229"
     CDS             53183..54037
                     /locus_tag="VW157_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEU9551599.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELIPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYINNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            54063..54833
                     /locus_tag="VW157_000230"
     CDS             54063..54833
                     /locus_tag="VW157_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9551600.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            54820..55686
                     /locus_tag="VW157_000231"
     CDS             54820..55686
                     /locus_tag="VW157_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnII"
                     /protein_id="HEU9551601.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEETKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(55773..57035)
                     /locus_tag="VW157_000232"
     CDS             complement(55773..57035)
                     /locus_tag="VW157_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9551602.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPIP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(57035..57616)
                     /locus_tag="VW157_000233"
     CDS             complement(57035..57616)
                     /locus_tag="VW157_000233"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9551603.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     regulatory      complement(58016..58111)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(58494..58733)
                     /locus_tag="VW157_000234"
     CDS             complement(58494..58733)
                     /locus_tag="VW157_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9551604.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     gene            complement(58778..58876)
                     /locus_tag="VW157_000235"
     CDS             complement(58778..58876)
                     /locus_tag="VW157_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9551605.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEY"
     gene            complement(58888..59715)
                     /gene="xth"
                     /locus_tag="VW157_000236"
     CDS             complement(58888..59715)
                     /gene="xth"
                     /locus_tag="VW157_000236"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9551606.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            59849..60305
                     /locus_tag="VW157_000237"
                     /pseudo
     CDS             59849..60305
                     /locus_tag="VW157_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            60302..60439
                     /locus_tag="VW157_000238"
     CDS             60302..60439
                     /locus_tag="VW157_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551607.1"
                     /translation="MTLNYIEILIKLVLTLKWLNNNVHFVKRLIDGKPTLLIKNGNID
                     P"
     gene            60458..60754
                     /locus_tag="VW157_000239"
     CDS             60458..60754
                     /locus_tag="VW157_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9551608.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGTVTVVTYE"
     gene            60960..62705
                     /locus_tag="VW157_000240"
     CDS             60960..62705
                     /locus_tag="VW157_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9551609.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYTTSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILTPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            62695..64446
                     /locus_tag="VW157_000241"
     CDS             62695..64446
                     /locus_tag="VW157_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9551610.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRNIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMADTL"
     gene            64619..65311
                     /locus_tag="VW157_000242"
     CDS             64619..65311
                     /locus_tag="VW157_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9551611.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            65354..66580
                     /locus_tag="VW157_000243"
     CDS             65354..66580
                     /locus_tag="VW157_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9551612.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEMGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            67710..69869
                     /gene="pfbA"
                     /locus_tag="VW157_000244"
     CDS             67710..69869
                     /gene="pfbA"
                     /locus_tag="VW157_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9551613.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            69853..>70118
                     /locus_tag="VW157_000245"
     CDS             69853..>70118
                     /locus_tag="VW157_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551614.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNS"
BASE COUNT        20549 a        12854 c        15713 g        21002 t
ORIGIN      
        1 aggtcccgta gtgtagcggt tatcacgtcg ccctgtcacg gcgaagatcg cgggttcgat
       61 tcccgtcggg accgtttaag gtaacgcaag ttattttaga ctcgttagct cagttggtag
      121 agcaattgac ttttaatcaa tgggtcactg gttcgagccc agtacgggtc atatatgcgg
      181 gtttggcgga attggcagac gcaccagatt taggatctgg cgcttaacgg cgtgggggtt
      241 caagtccctt aacccgcatt aagatataac aaatgagccg gcttagctca gttggtagag
      301 catctgattt gtaatcagag ggtcgcgtgt tcaagtcatg tagccggcat ttttagatag
      361 aagaaaacag tgcgaacgta gttcagtggt agaacaccac cttgccaagg tgggggtcgc
      421 gggttcgaat cccgtcgttc gcttagagag gccggggtgg cggaactggc agacgcacag
      481 gacttaaaat cctgcgattg gtaacgatcg taccggttcg attccggtcc tcggcataat
      541 atggagcacc cttagctcaa ctggatagag tacctgacta cgaatcaggc ggttagaggt
      601 tcgactcctc tagggtgcat ttttctattt aactcgggaa gtagctcagc ttggtagagt
      661 acttggtttg ggaccaaggt gtcgcaggtt cgaatcctgt cttcccgatt catggcggtg
      721 tagctcagct ggctagagcg tccggttcat acccgggagg tcgggggttc gatccccttc
      781 gccgctataa tgatcttgtc ggacctttag ctcagctggt tagagctctc ggctcataac
      841 cgagtggtcg taggttcaag tcctacaagg tccatttgaa tattggagga ttacccaagt
      901 ccggctgaag ggaacggtct tgaaaaccgt caggcgtgta aaagcgtgcg tgggttcgaa
      961 tcccacatcc tccttttata ttattaacgc gggatggagc agctcggtag ctcgtcgggc
     1021 tcataacccg aaggtcgtag gttcaaatcc tgctcccgca ataaggctcg gtagctcagt
     1081 tggtagagca atggattgaa gctccatgtg tcggcggttc gattccgtct cgcgccattt
     1141 tattgatagt atttatgcgg gtgtagttta gtggtaaaac tacagccttc caagctgttg
     1201 tcgcgagttc gattctcgtc acccgctttg aactttgttc aaattaccaa gttttttaac
     1261 ttgggcgcgt agctcaggtg gttagagcgc acgcctgata agcgtgaggt cggtggttcg
     1321 agtccactcg tgcccattaa ttggagaatt actcaagagg ctgaagagga cggtttgcta
     1381 aatcgttagg tcgggtaact ggcgcaaggg ttcgaatccc ttattctccg ttttaatgag
     1441 agtttgtaac tctctttttt gtttttagag ccaataaaac ttcagatccg taaaacttaa
     1501 ttgaattact aatctgaagt tatttttata tcgtttatat tataattgtg ttattttact
     1561 tccaactatt gagagaaatt tcgccactat ttagccagat ttcttttccg ttatcacatt
     1621 gaactaaaag ttttccattt tctgagatgt ctttagcaag tcccttgtag tctttttgct
     1681 ctagtgtgaa agtgacttct tttcctagaa tgaatgactg ttttttgtat aggtataata
     1741 gctcttctgc tggtgtttcg aagaaagcac gccagatttc tatgatcaat tcattccttg
     1801 ttataggagc tgtagcttta aataagctgg cagctttttc ttttaattcc tgagggaagt
     1861 ctttaatagt gaaattgata cctactccaa taatgatatc tgtgactaag ccagtttcca
     1921 cagaggtcat tgcttcagta aggattcctc caattttatg attgtttaga tagatatcat
     1981 tgacccattt tatgtcgaca tctattaaag ttaggttctt aatagctttg tagacagctc
     2041 cagctacaag tagtgtgtag gatggtaatt tgtcataggg gagatttggt ttaagatgga
     2101 gtgtcatata aataccacct tgtggtgagt agaaggaacg ttgaaaacgg cctcggcctg
     2161 ctgtttgata ggaagctaga tagagggtat ttgcttcatg gcctaaatca attgcttctt
     2221 ttgcatctag ttgtgttgat tttgtttcgg gtttaaagct gactttaatt ggaagatttt
     2281 cttctagaat ctctggaaga ataaggtcac cattcatcaa tttatatcct ctatttttga
     2341 tactatcaat ttcaatgcct tcttgttcta gtcgcttgat ggctttccaa attgatgttc
     2401 ggctgaggga tagttcttct gcgatttttt ctccgctgat ataatcggtt tcttttgcta
     2461 gaatttggta gacggcttgg taggatttca tagtgtttcc tttctcacta gttggtattt
     2521 cgaatgttct tttcttgtat gacttggggt ctgattaaag tattgtttat aagcttttga
     2581 aaaatggagt ggatctgaaa aacctaccga gtatgcaatt accttgatgg actcttgggt
     2641 attttcgaga agttgtttag ctcggtgcat tcgaacgtat agtaagtatt cttttgggga
     2701 taaggtgtta aattctttga atacacttga taagtagctt ctgtgaacgg atagttcttt
     2761 tgctaaatct tgaattgtaa gtgattgagg atagtggcta tcaattaatc gtttgcattc
     2821 aagatagagt tggtaggttg atgaaatatt cttttttttc tgattgggag caatagttcc
     2881 cagatgaaac atcagttcat ggagttgtcc catgatatgg agttgagcta attcatttga
     2941 ttttgtaatc tgagcgaagc ggacaatgtc tgagattagt tttgcagtag actgggtatg
     3001 acaagtttca gattggatga gataggcttg gtcaaaaatt tgagaaagag caaaatagtc
     3061 aggagctttc actccagtga ttcctaacca gtagtaggac caaggttctt cactatctgc
     3121 ttgataaaag gttagttcct ctggttttaa tagaaagaaa tctccttctt ttaaatcaac
     3181 aattttaccc ttgtaatgaa atgttccttg tcctttggta atgtaatgta ggacgtatgt
     3241 atctcgaatt gctgggccaa aagagtaatt aggtgtgcat tcctcatatc cataaaagct
     3301 tagggaaagg tcgattgttc cagtctggta ttctgaaaaa actagcatgt gctacctcct
     3361 acctaacatt ttaccatatt cttgagacat ttttctattt tagaaagcga tttcagatga
     3421 taaaatagaa ccaagaaatc gaagaggtga agtaaatggg agttcgaata gagaataatc
     3481 tattttatgt tgagagtaaa aatctaagtt tgattgttga aaatcgtgat ggctacttac
     3541 tattgaaaca tttagggaag actattaaga actataaagg ttccaatagt gtttatgaaa
     3601 gagaccatgc cttttcagga aatccaacgg ctactaatcg aacctttagt ttagatactc
     3661 agcgacagat ttttggacaa catggtttag gagattttag gaaaccaact atacaggttc
     3721 agcatagtgt aactgaagta acagattttc gatttgtaga agcaaagatt ttaaaaggtc
     3781 agaatggtcc acagggctta ccttctccac atagcatgga cgatacagag actcttgtct
     3841 taatgttaga agattctaag gctcaactta gtttgacttt gtattatact tcttttgata
     3901 atgattcgac tattgctagc tatagtaaat tagagaataa tagtaatcag gaagttgtca
     3961 tccataaaga tttttctttt atggctgatt ttccagctgc agattacgaa atcgtaactc
     4021 tgcagggtgc ttatgctcgt gaaaagactg ttagacgtca acaggtagaa caaggaatct
     4081 tttcgattgg ttcgaaccgt ggggcttctg gtcatgctca aacacctgct cttctactat
     4141 gtgatcaagg agtcacagag gatgctggga atgtgtttgc tattcaacta atgtatagtg
     4201 gcaactttga agcctttgtt caaaagaatc aattgaatga agttcggctg gctatgggca
     4261 taaatccaga aaacttttct tggaagttag atcctgagga aaactttgaa acaccggtag
     4321 ctttagtgac cttttcagat aagggattaa ctggtgttag tcatgaaagt cagaattttg
     4381 tactgaagca cattctgcca agtaaatttt ctacaaaaga acgcccaatt ctaattaata
     4441 actgggaagc tacttacttt gactttcaga gagaaaaact gttagagtta gctgatgaag
     4501 ctaagaaagt tggaattgaa ctttttgtat tagatgatgg ttggtttggc aatcgttttg
     4561 atgataatcg tgctttaggt gactggagtg ttaatgagaa aaagctgggt ggaagcctag
     4621 aaagtctgat ttcagctatc catgaaagag gtttgcagtt tgggctttgg ttagaacctg
     4681 aaatgatttc tgtagatagc gatttgtatc gtaaacatcc tgactgggct attcaggttc
     4741 ctggctatga gcatacttat tctcggaatc aattagtact taatcttgcc aatcctcagg
     4801 tagtagaata cttgaaaagt gtcttagatc aactcctatc ttatcatgat attgattaca
     4861 ttaaatggga tatgaaccgc aatatcacta agctagggaa tggattaact tatctagaga
     4921 cacagatgca atctcatcag tacatgctgg ggctttacga actcgtttct tatctgacag
     4981 agaagcacag ccatattctc tttgagtcct gctctggtgg tggtggacga aatgatcttg
     5041 gtatgatgcg ctatttccca caagtctggg ctagtgataa tacagatgct attgcacgtt
     5101 taccaattca atacggttca tcctatctct atccaaccat ttctatgggg gctcatgtgt
     5161 cagcagtacc gaatcatcag atggggcgaa cgacaccatt agaaacacgt ggacatgtag
     5221 caatgatggg aaatttgggc tatgagcttg atttgacaag tttatcagat gaagagaaag
     5281 ctgagattgc taatcaggtg aacttgtata aagaattacg accagtagtt cagttaggac
     5341 aacagtatag actaattaat cctgatactg catccaatga agctgctgta caatttaatt
     5401 acaaaaatca aacgattgta acctacgttc gcgttttgtc agttgtagag accatggaaa
     5461 caactttaaa gttaaaagat ttggatgaag agggactata tgaattacag gaaaatggcg
     5521 aagtttactc aggtgcagaa ctcatgtatg cgggtttaac tgttatttta tcccaaggag
     5581 attttttgag taaacagtat atttttagaa gactataatt aaggtgtata aatttataaa
     5641 ggaggctttc caatgaaatg gtataaaaaa atcggacttc ttgcaactac aggtttagct
     5701 ttggttgggc tcggcgcttg ctccaactat ggtaaatctg cggatggcac agtgaccatc
     5761 gagtatttca accagaaaaa agaaatgacc aaaaccttgg aagaaatcac tcgtgatttt
     5821 gagaaggaaa accctaagat caaggtcaaa gtcgtcaatg taccaaatgc tggtgaagta
     5881 ttgaagacac gcgttctcgc aggagatgtg cctgatgtgg tcaatattta cccacagtcc
     5941 atcgaactgc aagaatgggc aaaagcaggt gtctttgaag atttgagcaa caaagactac
     6001 ctgaaacgcg tgaaaaatgg ctacgctgaa aaatatgctg taaacgaaaa agtttacaac
     6061 gttcctttta cagctaatgc ttatggaatt tactacaaca aagataaatt cgaagaactg
     6121 ggcttgaagg ttcctgaaac ctgggatgaa tttgaacagt tagtcaaaga tatcgttgct
     6181 aaaggacaaa caccatttgg aattgcaggt gcagatgctt ggacactcaa tggttacaat
     6241 caattagcct ttgcgacagc aacaggtgga ggaaaagaag caaatcaata ccttcgttat
     6301 tctcaaccaa atgccattaa attgtcggat ccgattatga aagatgatat caaggtcatg
     6361 gacatccttc gcatcaatgg ctctaagcaa aagaactggg aaggtgctgg ctataccgat
     6421 gttatcggag ccttcgcacg tggggatgtc ctcatgacac caaatgggtc ttgggcgatc
     6481 acagcgatta atgaacaaaa accgaacttt aagattggga ccttcatgat tccaggaaaa
     6541 gaaaaaggac aaagcttaac cgttggtgcg ggagacttgg catggtctat ctcagccacc
     6601 accaaacatc caaaagaagc caatgccttt gtggaatata tgacccgtcc agaagtcatg
     6661 caaaaatact acgatgtgga cggatctcca acagcgatcg aaggggtcaa acaagcagga
     6721 gaagattcac cgcttgctgg tatgaccgaa tatgccttta cggatcgtca cttggtctgg
     6781 ttgcaacaat actggaccag tgaagcagac ttccatacct tgaccatgaa ctatgtcttg
     6841 accggtgata aacaaggcat ggtcaatgat ttgaatgcct tctttaaccc gatgaaagcg
     6901 gatgtggatt aggagtagag agactatgaa aaaagtatta caaaaatatt gggcatgggc
     6961 ttttgtggtc atccccctct tgttacaagc aattttcttc tatgtgccga tgtttcaagg
     7021 agccttttac agttttacca actggacagg attgacttac aactacaaat ttgtcggctt
     7081 aaacaacttt aagctcctct tcatggatcc aaaattcatg aatgcgattg gctttaccgc
     7141 aatcattacg attgccatgg tggttggtga gattgcactc gggatcttca ttgcgcgtgt
     7201 cttgaactct aaaatcaaag gccaaacctt cttccgtgct tggttcttct tcccagctgt
     7261 tttatctggt ttgacagtgg ctttgatctt caagcaagtc ttcaactacg gtcttccagc
     7321 gattgggaat gcccttcata ttgaatttct ccaaaccagt cttttaggga ctaagtgggg
     7381 agcaatcttt gcggctgtct ttgtccttct ttggcaaggg gtggctatgc ccatcatcat
     7441 cttcctagct ggtttgcaat ctattccaac tgagattaca gaggcagcaa ggattgatgg
     7501 tgcgactagc aagcaagttt tctggaacat tgaattgcct tacttgctac caagtgtctc
     7561 tatagtcttt atcctagccc taaaaggtgg gctgactgcc tttgaccaag tctttgccat
     7621 gaccggtggt ggtccaaaca atgccacaac ctcacttggg ctcttggttt ataactatgc
     7681 ctttaaaaac aaccaattcg gttatgccaa tgccattgcc gtaatcttgt tcttcttaat
     7741 tgtagtgatt tcgatcatcc aattgagagt atctaagaaa tttgaaattt aagaggagaa
     7801 gcatgatgaa acaagatgaa agaaaagccc tgattggcaa atacattcta ttgattctag
     7861 gatcggttct gcttttagtg ccgctccttg ctaccctctt tagttccttc aaacccacta
     7921 aggatattgt agataatttc tttggctttc caaccaactt cacatgggac aactttagcc
     7981 gtctcttagc tgatgggatt ggaggctatt attggaactc tgtcgtcatc actgtcttgt
     8041 ctttacttgc agtaatgatc tttatcccta tggcagccta ctccatcgct cgcaatatga
     8101 gtaaaagaaa agcctttacc atcatgtata ccctcttaat cctcggaatc ttcgtacctt
     8161 tccaagtcat catgattccg attacggtta tgatgagtaa actcggtttg gctaatacct
     8221 ttggtttgat cttgctctac ttgacctatg cgattccaca gaccctcttt ctctatgttg
     8281 gctatatcaa aatctcgatt ccagaaagtc tggatgaagc agcagagatc gatggggcta
     8341 atcaatttac aacctatttc cgcatcatct tcccaatgat gaaaccgatg catgcgacaa
     8401 ccatgatcat caatgccctt tggttctgga atgacttcat gttgccactc cttgtcttga
     8461 accgggattc caaaatgtgg actctgcctt tgttccaata caactacgca ggccaatatt
     8521 tcaacgacta cggaccaagc tttgcctctt acgtggtcgg cattatcagt atcaccattg
     8581 tctatctctt cttccaacgc catatcattt caggaatgag caacggggca gtgaagtaat
     8641 actcaatgaa aatcaaagag caaactagga agctagccgc aggctgtact tgagtacggt
     8701 aaggtgaagc tgacgtggtt tgaagagatt ttcgaatagt ataagaagag ctcatggaat
     8761 ctatagtctt tgacacagag gaagagttca gcttgttgat aattctatgt agaaaacaag
     8821 aaagtagtac tttgtttatc acagtctata ttagtacgag tttgatgata aatagagatt
     8881 taaaatgaat tttatttatg aagctagatc atatgagact tggcttcaat tagaaaaagg
     8941 agagtaatga tgccaattca aaataaaacc atgttgatta cctattctga tagccttgga
     9001 aataatctta aagacttata tgataatttg gaagagcatt ttcgagatgc tattggagga
     9061 gttcaccttt taccattttt cccatcaaca ggtgatcgtg gatttgcgcc agttgactac
     9121 gacgaagtgg actcagcttt tggtgattgg gaggatgtta agcgtttagg tgagagatat
     9181 tatcttatgt ttgattttat gattaatcat atttctcgtc aatccaagta ttataaggac
     9241 tatcaagaaa aacatgaagc cagtgaattt aaagctctct ttttaaactg ggataagttt
     9301 tggccagaaa accgtccgac acagtctgat gtagatttaa tttacaagcg taaggatcgt
     9361 gcaccaaagc aagagattgt gtttgaagat ggttcagtgg aacatttgtg gaataccttt
     9421 ggtgaggagc agattgatct tgatgtgacc aaagaagtaa ctatggaatt tatccgtaag
     9481 accattcagc acttggcaag taatgggtgt gatttgattc gtctagatgc ctttgcttat
     9541 gcagtgaaga aattggatac taatgatttc tttgtggaac cagatatttg ggatttattg
     9601 gacaaagttc gagatatcgc tgctgagtat gggacagagc ttttacctga gattcatgaa
     9661 cactattcga ttcagtttaa aatagcagac catgattact atgtttatga ttttgctctt
     9721 ccaatggtga cactttatac tctttacagt tccagaacag agcgtttggc taagtggtta
     9781 aagatgagcc cgatgaagca atttacgacg ctagataccc atgatgggat tggagtagta
     9841 gatgtcaagg atatcctgac cgatgaggag attgactatg cttcaaatga actctataag
     9901 gttggagcca atgtcaaacg taagtactct agtgccgagt ataacaactt agatatctac
     9961 caaataaatt caacctacta ttcagcgctt ggagatgatg atgtcaagta ttttctcgct
    10021 cgtctaattc aagcttttgc cccaggtatt cctcaggttt actatgtggg tctattagca
    10081 ggcaagaatg acttgaaatt attagaagaa actaaagaag gtcgaaatat taattgtcat
    10141 tactatagca acgaggaaat agcaaaagaa gtgcaacgac ctgttgtgaa ggcccttctc
    10201 aatctatttt ctttccgtaa ccgttcagaa gcctttgatc tagaagggac tactgagata
    10261 gagacaccaa cagctcacag cattgtaatc aaacgtcaaa ataaagataa gtccgtaaca
    10321 gcagtagtag aaattgattt gcaaaatcag acttatcggg taattgagaa tggagttgaa
    10381 gtatcatttt gaagccttga tatggtggat aaaatagggt ttttattttg gaaaacgttt
    10441 cctttgtttt caaattgcta aaaaagtggt acaatatagg atagcttact attatctgaa
    10501 tcagctgatt tggagagaaa ggattcattt tgaaatcaat aggctttatt gaaaagctga
    10561 aggggttgtc tagtaaagag ctgattttat tgggaattat cctaagtatc tttttaccct
    10621 tttatctttt tgtagttgta ctctgtttat atattatcag tttgattttt acaggagaca
    10681 tgaaaagtat tcttcagaaa atgggggagc atccgatgct gcttcttttt cttggctata
    10741 gtactgttat atccattctt gcacaaaatt ggatgggtct tgtggcttca gtaggaatgt
    10801 ttctatttac tattttcttt ttgcactatc agtcgatttt atcccataaa ttctttcgat
    10861 tgattttgca gttcgtcttg tttggtagtg tcttgtcagc tgcttttgcc agtttagaac
    10921 atttccaaat tgtgaagaaa tttaactatg cttttctttc acccaatatg caggtgtggc
    10981 atcagaaccg ggcagaagtg accttcttta atcctaatta ttatggaatt atttgttgtt
    11041 tctgtattat gattgctttc tatctgttta caacgaccaa gttgaattgg ttgaaagtat
    11101 tctgtgtgat tgcaggcttt gttaatctct ttggtttgaa ctttactcaa aatcgaactg
    11161 cctttcctgc tattatcgct ggagcaatta tctatctctt tacgacgatt aaaaactgga
    11221 aggccttttg gcttagtatt ggagtcttcg cgattggttt gagtttcctc ttttctagtg
    11281 atttgggagt tcgaatgggt actttagact cttctatgga agaacgcatt tctatctggg
    11341 atgctgggat ggccttgttt aagcaaaatc ctttttgggg tgaagggcca ttgacctata
    11401 tgcactctta tcctcggata catgctcctt atcatgaaca tgcccacagt ctttatattg
    11461 atacgattct gagttacgga attgtgggta ccattttatt agttttgtct tctgttgctc
    11521 ctgttcgctt gatgatggat atgagtcagg agtcggggaa acgtccgatt atcggccttt
    11581 atctatcttt ccttacagtg gttgctgtgc acggaatttt tgacttggct ctcttctgga
    11641 ttcagtcagg ctttattttc ttgctagtta tgtgcagtat tccattggag catcgaatgt
    11701 tggtatcgga catgacggat taagttttat aagtttgaaa tcttctacct tgggtaggag
    11761 atttttttat acgatgggca tgtcgtgcat ctgaggtgta agtcctctgt agggatccac
    11821 taccggtgaa tacaatagcg ctccccaagc ctgactatcg tgaggtagcg gattaaaatg
    11881 gtctggggat agatcgtttt aagtctgacg caggaaataa gaattgtcag agaaagcaat
    11941 agtgaaatcg taggctatga atagaaacgt gatagcgagg agcatatagt ggataaggta
    12001 gcgagaaatt ttaaagtata aaagatagtc ttaatctata tatgtaaatc acaaaagtaa
    12061 ttgattcgga ataaggttgg tgtgaaaaga catagtaaac tgaatcttga atactagaat
    12121 gatacttcta caatattgtt ataatttaat ttgaattcca aaatcacatt gttccgtccg
    12181 tatttcattt tactgtatgt cttcctaagc tccaaaattt ctgtgtcaaa tttcctattt
    12241 tcactgtagt ctttgacact tcgaacgaca atttgaatca tatattcaaa aaaaaaatga
    12301 aaaatttgtg ccaacaagcc taaatattct gaaaatttgt ttgattttta atggataatg
    12361 tgatataatg gttgtcaatc tataattcac ataaacagag gagtagaaaa tgaaaaaaaa
    12421 tcgtgtattt gctacagcag gtcttgtttt attagcagca ggtgtacttg cagcatgcag
    12481 ttcttcaaaa tcatctgatt catcagcccc taaagcttat ggctatgttt atacagcaga
    12541 cccagaaacc ttggactacc tgatttcaag taaaaatagt acaacagtag tgacttcaaa
    12601 tgggattgat ggtttattca ctaacgataa ttacggtaat cttgctcctg cagttgcaga
    12661 ggattgggaa gtctctaagg atggtttgac ctacacttat aagattcgta aaggggttaa
    12721 atggtttacc tctgatggag aagaatatgc agaggtgacg gctaaagatt tcgtgaacgg
    12781 tttaaaacac gcagcagata aaaaatcaga agctatgtat ttagctgaaa attcggttaa
    12841 aggcttggca gattatctat caggaacttc aacagatttt tcaacagttg gtgtcaaggc
    12901 ggttgatgat tatacgttac aatacacttt gaaccagcct gaaccgttct ggaactctaa
    12961 gttgacctat tctattttct ggcctctgaa tgaagaattc gaaacatcaa aaggaagcga
    13021 ttttgctaaa ccaacagatc cgacatcctt gctttataat ggtccattct tgttgaaagg
    13081 gttgactgca aaatcttctg tagagtttgt aaaaaatgag caatattggg ataaagaaaa
    13141 tgtccaccta gatactatca atctagctta ctatgatgga tcagatcagg agtcgctaga
    13201 gcgtaacttc actagtggag cttatagtta tgcccgtctt taccctacca gctccaacta
    13261 ttctaaggtt gcagaagaat acaaggacaa tatctattac acacaatcag gctctgggat
    13321 tgctggtctg ggtgtgaata ttgatcgcca aagttacaac tatacttcta aaactacaga
    13381 ttcagagaaa gtagctacta agaaggcatt gcttaacaaa gatttccgtc aagccttgaa
    13441 ttttgctctt gatcgctcag cttactcagc tcaaatcaat ggtaaagatg gagcagcttt
    13501 agcagttcgt aatttatttg taaaaccaga ctttgtttca gctggtgaga agacctttgg
    13561 tgatttagtc gctgctcaac ttcctgctta tggtgatgag tggaaaggtg tgaatttagc
    13621 tgatgggcag gatggtttat tcaatgctga caaggccaag gcagagtttg cgaaagctaa
    13681 gaaagcttta gaagcagacg gcgttcagtt tcctattcat ctggacgttc cagtagacca
    13741 agcatcaaaa aactacatat ctcgtattca gtcctttaaa caatctgtag aaacagttct
    13801 tggtgttgaa aatgtcgttg ttgatattca acaaatgaca agtgatgaat tccttaatat
    13861 tacttactat gctgccaatg cttcatctga ggattgggat gtatcgggag gagtttcatg
    13921 ggggccagac tatcaagacc catctactta cctggatatt ttaaaaacaa ctagcagtga
    13981 aactacaaaa acatatttag gatttgataa tccaaatagc ccttcagtag ttcaagttgg
    14041 tttgaaagaa tacgataaat tagttgatga agctgccaaa gagacaagcg acttgaatgt
    14101 ccgttatgaa aaatatgcag cggctcaagc atggttgaca gatagttcac tctttattcc
    14161 tgctatggct tcttctggtg cagcaccagt gctttcacga attgttccat ttactggagc
    14221 ttctgcgcaa acaggctcta aggggtcaga tgtttacttc aaatatttga aattacaaga
    14281 taaagtggtg actaaggaag agtatgaaaa agctcgtgaa aaatggttga aagaaaaagc
    14341 tgaatcaaat gagaaagctc aaaaagaatt ggcaagtcat gtgaagtaaa taatttctaa
    14401 tagaactttc tctccatgag gagaaggttc ttttgggatt tttaaaggaa ataatatgaa
    14461 aaaatatatt tttatgcgtg ttttgcggtc attggtttcg attttcttag taacgacttt
    14521 gacctacacg attatctata ccttggttcc tcgaaaattg attttcaagc aggatcctaa
    14581 ctataataaa attgcgacaa cggctgataa acgtgataac tatgaaaata ctgtgtttga
    14641 gcgtatgggc tacattgagt attacgatac taaagagttg caagaaaagg caagtagcat
    14701 ggattcttct gtaacagtag aagcaaatgc gaccaataaa gctatttatg aaaagtacat
    14761 caatcaatta ggtcatggtt ggactttggg agaatttact gaaagtggtc aattctatgc
    14821 tactcgtgaa attccaattt ttgaacgtgt ttttcacttc tatgctaact tgattgacat
    14881 tgaccataca aataaaatcc aagaccctga aaatccagac ttgaaacgct accttcgttt
    14941 tgaaaatgat ccagctatcg gatggtcatt ggtcggttca ggaactaaac ataaatatct
    15001 cttgtacttt aacagtcagt tcccatttgt tcatcaaaac tttgtgaact tgaatttagg
    15061 tgactcttac ccaacctatg ctaatacacc agttcttcag gttattactc aaggtcaagg
    15121 acaaaccaaa actgcccaag ttcagttccc aacaggtaag aaaacgtctt ctgtaaatat
    15181 ttactcaaga acctacaagt cacctagtca ggctgactct cgtgaagtag ctagctatgg
    15241 gaaagatgat ccttatacag cgactgaaag taattaccaa tatccatcta tgattgtcag
    15301 ctctgctatt actggtttga ttggtttggt tcttgcctat gctcttgccg tgccacttgg
    15361 ttcagccatg gctcgtttca agaacacttg gattgatagc ctctcaacag gggctttgac
    15421 cttcttgctt gctcttccaa cgattgcctt ggtttacatc gttcgattga ttggatcatc
    15481 tattgccctt ccagattcat tccctatctt gggagctgga gattggcgtt cttacgtttt
    15541 accagcagtc atccttggtt tgttgggtgc tcctggtaca gccatttgga ttcgtcgtta
    15601 catgattgac ttgcaatctc aagactttgt tcgtttcgct cgtgcaaaag gtttgtctga
    15661 aaaagaaatt tcaaacaaac acatctttaa aaatgccatg gttccgctgg tttcaggaat
    15721 tcctggtgcc atcattgggg ttatcggtgg cgcaaccctt actgaaacag tcttcgcctt
    15781 cccaggtatg ggtaaaatgt tgattgactc tgtaaaagca tctaataact ctatggtcgt
    15841 tggtcttgtc ttcatcttta catgtatttc tatcttctca cgtcttttgg gagatatttg
    15901 gatgactatt attgacccac gtattaaatt gactgagaaa ggaggcaaat aatgtctaca
    15961 atcgataaag aaaaatttca gtttgtaaaa cgtgacgatt ttgcctctga aactattgat
    16021 gcgccagcat attcttactg gaaatcagtg tttaaacaat ttatgaagaa aaaatcaact
    16081 gtagtcatgt tgggaatctt ggtagccatc attttgataa gtttcatcta cccaatgttt
    16141 tctaagtttg atttcaatga tgtcagcaag gtaaacgact ttagtattcg ttatatcaag
    16201 ccaaatgcgg agcattggtt cggtactgac agtaacggta aatcgctctt tgacggtgtc
    16261 tggttcggag ctcgtaactc catcctcatt tctgtgattg cgacagtgat taacttggtt
    16321 atcggtgttt ttgtcggtgg tatttggggt atttcaaaat cagttgaccg tgtcatgatg
    16381 gaagtttaca acgtcatctc aaacatccca cctcttttga ttgttattgt cttgacttac
    16441 tcaatcggag ctggattctg gaatctgatt tttgccatga gcgtaacaac atggattggt
    16501 atcgccttca tgatccgtgt gcaaatcttg cgctatcgtg acttggaata caacttggcg
    16561 tcacgtactt tgggaacacc aaccttgaag attgttgcca aaaatatcat gcctcaattg
    16621 gtatctgtta ttgtgacaac catgactcaa atgcttccaa gctttatctc atacgaagcc
    16681 ttcttgtctt tcttcggtct tggattaccg attacagtgc caagtttggg tcgtttgatt
    16741 tcggattatt cacaaaacgt aacaaccaat gcttacttgt tctggattcc attgacaacc
    16801 cttgtcttgg tatccttgtc ccttttcgta gttggtcaaa acttagcgga tgctagtgat
    16861 ccacgtacac atagatagga gtagaaatga caaaagaaaa aaatgtaatt ttgactgctc
    16921 gcgatattgt cgtggaattt gacgttcgtg acaaagtatt gacagccatt cgcggcgttt
    16981 cccttgaact agttgaagga gaagtattag ccttggtagg tgagtcagga tcaggtaaat
    17041 ctgttttgac aaagaccttc acaggtatgc tcgaagaaaa tggtcgcatt gcccaaggta
    17101 gtattgacta ccgtggtcag gatttgacag ctttatcttc tcacaaggat tgggaacaaa
    17161 ttcgtggtgc taagattgcg actatcttcc aggacccaat gactagtttg gaccccatta
    17221 aaacaattgg tagtcagatt acagaagtta ttgtaaaaca ccaaggaaaa acagctaaag
    17281 aagcgaaaga attggccatt gactacatga ataaggttgg cattccagac gcagatagac
    17341 gttttaatga atacccattc caatattctg gaggaatgcg tcaacgtatc gttattgcga
    17401 ttgcccttgc ctgccgacct gatgtcttga tctgtgatga gccaacaact gccttggatg
    17461 taactattca agctcagatt attgatttgc taaaatcttt acaaaacgag tatcatttca
    17521 caacaatctt tattacccac gaccttggtg tggtggcaag tattgcggat aaggtagcgg
    17581 ttatgtatgc aggagaaatc gttgagtatg gaacggttga ggaagtcttc tatgaccctc
    17641 gccatccata tacatggagt ctcttgtcta gcttgcctca gcttgctgat gataaagggg
    17701 atctttactc aatcccagga acacctccgt cactttatac tgacctgaaa ggggatgctt
    17761 ttgccttgcg ttctgactac gcaatgcaga ttgacttcga acaaaaagct cctcaattct
    17821 cagtatcaga gacacattgg gctaaaactt ggcttcttca tgaggatgct ccgaaagtag
    17881 aaaaaccagc tgtgattgca aatctccatg ataagatccg tgaaaaaatg ggatttgccc
    17941 atctggctga ctaggaggaa ggaaatgtct gaaaaattag tagaaatcaa agatttagaa
    18001 atttccttcg gtgaaggaag taagaagttt gtcgcggtta aaaatgctaa cttctttatc
    18061 aacaagggag aaactttctc gcttgtaggt gagtccggta gtgggaaaac aactattggt
    18121 cgtgctatca tcggtctaaa tgatacaagt aatggagata tcatttttga tggtcaaaag
    18181 attaatggta agaaatcgcg tgaacaagct ccggaattga ttcgtcgaat ccagatgatt
    18241 ttccaagacc ctgccgcaag tttgaatgaa cgtgcgactg ttgattatat tatttctgaa
    18301 ggtctttaca atcaccgttt atttaaggat gaagaagaac gtaaagagaa agttcaaaat
    18361 attatccgtg aagtaggtct tcttgctgag cacttgactc gttaccctca tgaattctca
    18421 ggcggtcaac gtcaacgtat cggtattgcc cgtgccttgg tcatgcaacc agactttgtt
    18481 attgcagatg agccaatttc agccttggac gtttctgtac gtgcccaagt cttgaacttg
    18541 ctcaaaaaat tccaaaaaga gctcggcctg acctatctct tcatcgccca tgacttgtcg
    18601 gttgttcgct ttatttcaga tcgtatcgca gttatttaca agggtgttat tgtagaggtt
    18661 gcagaaacag aagaattgtt taacaatcca attcacccat atactcaagc cttgctttca
    18721 gcggtaccaa tcccagatcc aatcttggaa cgtaagaagg tcttgaaggt ttacgatcca
    18781 agtcaacacg actatgagac tgataagcca tctatggtag aaatccgtcc aggtcactat
    18841 gtttgggcga accaagccga attggcacgt tatcaaaaag gactaaacta gtaatggttt
    18901 tataatttcc atgtcaattt ttatggaaat tataaaactt tttttgttgg actgatttga
    18961 aaaactttga aagcttcctg aaagaatttt tgaaaaattt taaaaattat gaaatattat
    19021 tgacaggtga cgaaaaaggt ggtaggatag gaagcatcaa aaaagaaagc agaaaaagaa
    19081 atgaataaga gacaagccgt aactatgaat caaaactttt actttagcta ctttagatag
    19141 tgtttgtaga catgtcacca gggatgcaaa tagttcaagc aatttgtttg tatctatgtg
    19201 gttaggattt ttgattgtgg tccatataga tgaatatcta aatggactag ctaagttgta
    19261 acttgttagg ttatttcaag catccgttta gatagtatct aggcggtttt ttttatcttt
    19321 tctgagaagg agtttcatta tgaaagttgt tcgaaaatta ctagcccccc tcttggtagt
    19381 ggggatcctc ttgacttctc tgatcagttt gcatcagttg aaggcagata agaaaaaaga
    19441 tgtctttcgt atcggtattt cgcagtttat tacccaccag tccttagacg ctactagaga
    19501 aggatttgtg gatgagctga ccaaacaagg ctatgttgag ggggaaaata ttgagattga
    19561 tttgcaaaat gcacagggag aacaaagaaa tctaaaaacg atttcccagc aactagcaga
    19621 atccagcgat gtcgttctag ccatcgcaag gccttctgct cagagcttgg ccaatacgac
    19681 gcaaacgaca ccagttatct tttcagctgt aacagaccca gtcagtgcca agttggttga
    19741 gtcaagagaa catcctgggg gcaatgtaac tgggacaagc gatcagtcat cagatgccat
    19801 ttcaacccaa atcaatttga taaagaaagt gttgctaaag gctaaaacga ttggaatcct
    19861 ctatactcag agcgagccca attcggttgt ttaaaaggat gaagctaagc gacttctgaa
    19921 agaaaaaggc tttaccgttg ttgaaaaaac aatcttggac agtaataaca tcaaggcggc
    19981 agcagagagt ttgatggcag aggtggatat ggtttttgtc ccaactgata atatcatttc
    20041 gtcaaccatg gaaacagtta agcaggtttc tattaaacac aaggttccag tattcggtgg
    20101 ttcaacagaa atgattgctg ttggtgactt gtataactac ggaaccaatt atgaagaatt
    20161 gggaagacag acagctcgca tgctgattcg tgttttaaaa ggtgagaaac cagaaaatat
    20221 agcagttgag ttgcctgaaa aactggaatt gcatactaat caagaaatgg cagatgcatt
    20281 gggaattgat attagtaagt tagaaggcaa ggaataagga ggattaagat gaattttgtg
    20341 ttatctagtt tatcagaagg tttgttgtgg tcgattgtgg cgattggggt ctacttgact
    20401 ttccgtattt tggatattgc ggatatgact gctgagggag cctttcctct gggggcagca
    20461 gtcgttgtat cacagataca ggcaggggca aatccttggc ttgcgatttt acttgctttg
    20521 ctggcaggta tggtagcagg tcttgtatca ggaatgctcc acaccaagat gaaaatccca
    20581 gctctcttga cagggattgt gaccttgaca gggctctatt caatcaatat taaaatcatg
    20641 ggcagtgtac tcaatctttc cttgggagat tctgcaaccg tctttaaaca gttggcgagc
    20701 ttgggtctga caaatgaagg agctgttttc tcacttagtt tagtctgtct cttacttgtt
    20761 tgttcttgac ccttttgatg aaaacagaga ttggtttggt cttgcgttcg actggggata
    20821 atatcccgat aagtgaggcc aatggagtca atgtagacac catgaagatt gttggttaca
    20881 tgatttcaaa cggtttgatt gccctatgtg gttccttgtt tgcccaaaat gatggatttt
    20941 cagatgtgac ttctgggaca ggaaccattg ttgttggttt gagctcggaa gtcttgatac
    21001 acgacttgat cattggaggc cgcttattat ccatcggaat cggtgctatt gtttaccgtt
    21061 tgattatttt aaatatctat gaaattccaa atctagatca aaacttggta cgtctcttta
    21121 atgcaatctt gctcgctttg gttttatttg caccagaatt gcaaaagaga ttaaagattc
    21181 gtggtctgaa attgagaaat gaataggagg agaaagttat ggcaagtttg ttaacacttg
    21241 aaaatattca caagacattt gaagcaggga cggtcaatga gaaccatgtc ctcaaaggat
    21301 tagatttaga ggttgaagag ggagatttta tctatgtgat tggtggaaat ggagcaggaa
    21361 aatccacttt gatgaatatc ttggctggca atctatcggt ggacgaaggg gaccttctac
    21421 tggcagggaa atccattaaa aatctgagtg tcagaaagag agccaaggat attgcccgtg
    21481 tttttcaata tcctaagatg ggaacagctt ctcgtttgac tattgaggag aatatggcta
    21541 ttgccttgag acgtgggcaa aagagagggc ttggttgggg tgtgaaggag aaggatagaa
    21601 tccagttcca agaggccttg aaggagttga atattggtct tgaaaaccgc ttaaaagtag
    21661 atactcaata tctctcagga ggacaaagac aggctttgac cctagtcatg gcagctctgg
    21721 tgaaacccaa acttttactt ttggatgaac atactgcggc acttgacccg aaaaccagtc
    21781 aaatggtgat ggacttgacg caaaagattg tggagcacca tcagtggacg actttgatga
    21841 ttacccatga tatgaatcat gcgattgagt acgacaatcg tctcattatg ctctatcaag
    21901 gcaagatagt ggtggatgtc aagggagaag agaaaaagca tctgacagtt gaagacctta
    21961 tgcatctctt ccagaaaaat agtggccaaa gtctggtcag tgatgaatta gttttgggat
    22021 aaagaaaaag gctgagattg gtgtctcagt cttttatttc ttgcgttttg caaaatctac
    22081 aaatctaaat ttttctaact tatggcgact ttcggtaaac tgaaattggc gtccgtcttg
    22141 gagatagact tttgatttaa tcgaaacgac ataagggtct ttgccaatgt ccatgagaat
    22201 cttgtctcgg tcgcttgagt ggtcaatggt gatttccttc tgagcataat caataaggag
    22261 tttgaggcca ttttctatat aagaatagat agactgctca gcaatttggc gagtgagatt
    22321 tgggattagt tccatatcca gatagtccgt atccagaacg gataccagat catccaccac
    22381 acgctggcgg accaccttcc aaaccatccg aaactctggg aaaccggtta tcagtgagga
    22441 ttttttatca ataataatct tgtccagact gaccacgttg gttttagagc gcagtccaag
    22501 ttctttaact agttcttggt agctggttag gttggataca gggaaattga cggtttcttc
    22561 tttgacgact tgagaacctt gccctcttat ctttttgatc aatccttcct cttggaggag
    22621 agacagggcc tttcggacgg tatcacgact cacttgatat tgctccataa ggtcgtgctc
    22681 gctaggaagg aaatctccga cagcgtaagt ctcgttttga atggtttctt ggatttgctt
    22741 aaataattgt tggtatttct tcattttgcc tgcttaaata ggtttttagt ctagtttaat
    22801 gaacacctgt atctatttta ccatacaagt gctagaaaat ttacaaaaaa acaccctttt
    22861 tacaacaaac aagttgcaga caagtttggt atcgtttaca atatacttat caaatcaaac
    22921 ttgctttgac aagtataagg agaatcaaat gggaaaattt gaacaagaag ccaaagatct
    22981 gcttcaggca attggaggca aagaaaatgt gattgccgta actcactgtg cgacacggat
    23041 gcgctttgtt ttaggagatg ataagaaggc taatgttaaa gctatcgagt caattccagc
    23101 tgttaaagga acctttacaa atgcaggtca atttcaggta atcattggaa atgacgtgcc
    23161 catcttttat aatgatttta cagccgtttc aggtattgag ggtgtttcca aagaagcagc
    23221 caagtctgca gctaagagta atcaaaacgt ggtccaaggt gttatgacca ctctggcgga
    23281 gatttttact ccgattattc cagccttgat agtcggagga ttgatcctcg gtttccgtaa
    23341 tgtcttggaa ggtgtccatt ggtcgatgtt ggatggcaag accatcacag aatcctctca
    23401 gttttgggca ggtgttaatc atttcctctg gttgcctgga gaagctatct tccagttctt
    23461 accagtaggg attacttggt ctgtttctcg taagatggga accagccaaa ttttgggaat
    23521 tgttctcgga atctgtttgg tatcgcctca gttgctcaat gcctatgcgg ttgcttcaac
    23581 gccagcagct gatatcgcgg caaactgggt ttggaatttt ggctatttta ctgttaatcg
    23641 tatcggttac caagcccaag ttatcccagc cttgcttgca ggtttgagtc tgtcttatct
    23701 tgaaatcttc tggcgcaagc atatcccaga agtcatttct atgatttttg tacctttctt
    23761 gtcattgatt ccagccttga ttttggctca tactgttttg ggaccaatcg gttggacaat
    23821 tggacaagga ctttcatcag ttgtcttggc aggtttaact ggtccagtta aatggctctt
    23881 cggtgcaatt tttggtgccc tctacgctcc atttgtcatc acaggtctgc accatatgac
    23941 caatgccatt gatacacaat tgattgcgga tgctggtggc actgccctat ggccaatgat
    24001 tgctctttct aatattgctc aaggctcagc cgtgtttgcc tattatttca tgcatcgcca
    24061 tgatgagcgt gaggctcagg tttcacttcc tgcaaccatt tcagcctatc tcggtgttac
    24121 agaaccagct ctttttgggg ttaacgtaaa atatatttat ccatttgttg ctgggatgac
    24181 tggttcagcc cttgcaggca tgttatccgt tacttttaat gtaactgcgg cttctattgg
    24241 tatcggtggt ttgccaggta ttctctctat tcaacctcaa tacatgctgc catttgcagg
    24301 aactatgcta gttgcgattg ttgttccaat gctcttgact ttcttcttcc gcaaggctgg
    24361 tctctttaca aaaacagagg gcgatacgaa cttgcaggca gaattcgttg ctcaagaaga
    24421 agcagaattt gtgaaccatg aaccagtaga acttacttcg gtagaaatta tcagcccact
    24481 aactggccaa gtgaaagaat tgagtcaagc gacggatcct atttttgcat caggtgtcat
    24541 ggggcaaggt ctagtcattg aaccaagcca aggtgagttg acctctccag ttaatgggac
    24601 agtgacggtt cttttcccta ccaagcatgc catcggcatt gtctctgacg agggagttga
    24661 attgctcatc cacatcggta tggatacagt aggtcttgat ggcaaaggtt ttgaaagtct
    24721 tgtagtccaa ggagatcacg ttacagttgg tcagcaactg attcgttttg atatggatgt
    24781 cattaaggct gcaggtctgg tgacagaaac tcctgttatc atcaccaacc aagatgctta
    24841 tacagcgact attcccggaa cttatccgac aacgatccaa gctggagcat ctctcatggt
    24901 cgctacacga atctaatact cttcgaaaat ctcttcaaac cacgtcagct ctatctgcaa
    24961 cctcaaaata gtgttttgag caacctgtgg ctagcttcct agtttgttct ttgattttca
    25021 ttgagtataa ttgaaatagc acaaataggg acggaagact gcttcctccc ttttatcaga
    25081 aaaggagaaa cagatgacac ttgataaagg aaaagtagtc tatcaaatct atccaaaatc
    25141 ttacaaagac accactgaaa acggtattgg ggatttccgt gggattatcg aaaaaattcc
    25201 ctatttagcc aaactaggtg tggatatggt ctggctcaat ccattctatc caagccctca
    25261 acgggataat ggttacgata tttcagatta tatggcagtg gatcctcttt ttggtgatat
    25321 ggctgatttt gaggaaatgg tgtgtgtcgg taaagagcac aagattgact ttatgctgga
    25381 catggtactt aatcattgtt cgacagagca tgaatggttt cagaaagccc tagctggtga
    25441 taagtattat caagactttt tcttcatcca agaccaacca acagattggc agtctaagtt
    25501 tggtggttct gcatggacgc ctttcgggga tactgggaaa tactatcttc acctatttga
    25561 tgagacccag gctgacctta actggcgcaa ttccaatgtc cgtaaggagc ttttcaaggt
    25621 tgttaatttc tggcgcgaca agggtgtcaa aggtttccga tttgatgtga tcaatttgat
    25681 cggtaaggac gaggtttcag tggactgtcc tgaaaatgaa gggaagccag cttatacaga
    25741 caaacccatc gttcataact atttgcgtat gatgaaccaa gccacttttg gatccgacga
    25801 tagctttatg acagttgggg aaatgtcttc taccaccatg gaaaactgtg tcctctattc
    25861 gtcgcctgat cgtcaggaat tatccatgac ctttaatttt catcacctca aggtggacta
    25921 taaggatgga caaaagtgga ccttggctcc ctttgatttt gaagagttga agagtcttta
    25981 ccatagttgg ggtaaggaaa tgagtgataa agacggttgg agtgccctct tttggaacaa
    26041 tcacgatcaa ccacgtgcct tgaaccgttt tgtcgatatt caaaacttcc gcaaggaagg
    26101 agccaccatg ctagcagcca gcattcacct gtcacgtggg acaccttata tctacatggg
    26161 tgaggaaatc gggatgattg acccagacta tgattctatg gctgattatg tggatgtcga
    26221 atcgcttaat gcctatcaga tgctcttaga agaaggaaag agtcagcaag aagccttcca
    26281 gattattcaa gctaagtcgc gtgataattc acgcattccc atgcagtggg atgcttcgga
    26341 aaatgcagga ttttcaacag gcactccttg gttgaaagct ggtaaatcct ataaatacat
    26401 caatgtagaa aatgaaatcc agggtccgat ttttaccttc tatcaagact tgattcgact
    26461 gcgtaaggaa atgccaatca tttcagaagg aagttacaaa ccagcctttg aagatagcaa
    26521 acaagtctac gcttttgaac gccagtttga ggatcaaaag ttactagtgc tcaataactt
    26581 ttatgctaaa gaagtgggaa tcgacttgcc agcagtctac caaaatggac agattttgat
    26641 atcaaactat gaagatgcag aagtgtctga gaaaattcta ctcaaaccct atcaaacact
    26701 ggctatttat gtaaattaat actcttcgaa aatctcttca aaccgtgtca gttttatctg
    26761 caacctcaaa gcggtgcttt gagcaacctg tggctagctt cctagtttgc tctttgattt
    26821 tcattgagta taaacttagc aaggagacct ctttttcaga aaagcagggt tctttttgct
    26881 gtctattcca tccctgaaag tgtagaacaa actggtcagg gtggctagtt tgtggtataa
    26941 tgaaagggac tcaaaaagaa acaggagaaa aatgatggat ttacttttag caattgtatt
    27001 gattgtgcta gcttttctag gaggagctct tggaggaatg tacttggttc gtaagcaaat
    27061 cgaaaaagaa tttgctgaca acccacgttt gaatgctgaa gcagttcgta ctcttttgag
    27121 tgcaaatggt caaaaaccaa gcgaagctaa ggtacaacaa gtttaccacc aaatcatccg
    27181 ccaacaaaag gcagcccttg ctaacaataa aaagaaaaaa taaataagga aaagtctggg
    27241 atgaaagttc cagacttctc actatgttgg ttatggttta gggatgagac tttttccttg
    27301 cattctattg atatttgatt atgattaaga gagaggtagt tgacattcta tcaagctgtc
    27361 ggtcagttcg ttcagtacaa ggaatcataa tgaacgatca atcagaaaaa agactagaaa
    27421 gaagactgta tggataatcg accaattggt tttttggatt cgggtgtcgg gggcttgacc
    27481 gttgtgcgcg agctcatgcg ccagcttccc catgaagaaa tcgtctatat tggagattcg
    27541 gcgcgggcgc cctatggccc ccgtcctgct gagcaaattc gtgaatatac ttggcagctg
    27601 gtcaactttc tcttgactaa ggatgtcaaa atgattgtca ttgcttgtaa cactgcgact
    27661 gcggtcgtct gggaagaaat caaggctcaa ctagatattc ctgtcttggg tgtaattttg
    27721 ccaggagctt cggcagccat caagtccagt caaggtggga aaatcggagt gattggaacg
    27781 cccatgacgg tacaatcaga catataccgt cagaaaatcc atgatctgga tcccgactta
    27841 caggtggaga gcttggcctg tcccaagttt gctcccttgg ttgagtcagg tgccctgtca
    27901 accagtgtta ccaagaaggt ggtctatgaa accctgcgtc ccttggttgg aaaggtggat
    27961 agcctgattt tgggctgtac tcattatcca ctccttcgcc ctattatcca aaatgtgatg
    28021 gggccaaagg ttcagctcat cgatagtggg gcagagtgcg tacgggatat ctcagtctta
    28081 ctcaattatt ttgaaatcaa tcgtggtcgc gatgctggac cactccatca ccgtttttac
    28141 acaacagcca gtagccaaag ttttgcacaa attggtgaag aatggctgga aaaagagatt
    28201 catgtggagc atgtagaatt atgacaaata aaatttatga atataaggat gaccaggact
    28261 ggtatgttgg gtcttatagt atttttggtg gcgttaacag tttgagcgac tataagacag
    28321 attttcctct gtttgaattc tccaaaatat ttggagatga agagtatggt ttcccgcttt
    28381 cagttactgt tttacgctat ggttctacct accgtttgtt ctcctttgtg gtagacatgc
    28441 ttaatcaaga aatgggacga aacttggaag ttattcaacg tcatggggcc ctgctcttgg
    28501 ttgaaaatgg gcaactcttg tatgtagaat tgcctaaaga aggggtcaat gttcatgatt
    28561 tctttgagac aagcaaggtc agagaaacct tgttgattgc gactcgtaac gaaggcaaaa
    28621 ccaaggaatt ccgagctatc tttgataagt taggctacga tgtggaaaat cttaatgact
    28681 accctgacct gcctgaagta gcagaaacag gtatgacctt tgaagaaaat gcccgcctta
    28741 aggcggaaac catttctcaa ttaacgggca agatggtttt ggcagatgat tctggtctca
    28801 aagtcgatgt ccttggtggc ttaccaggcg tctggtcagc tcgtttcgca ggtgtgggag
    28861 caactgaccg tgaaaataat gccaaactct tgcacgaatt ggccatggtc tttgaactca
    28921 aggaccgctc ggctcagttc cacacaaccc tagtcgtagc cagcccaaat aaggaaagtt
    28981 tagttgttga agcagactgg tcaggttata ttaactttga acctaagggt gaaaatggct
    29041 ttggctatga tcccctcttc cttgtaggag agacaggtga gtcatcagct gaattaaccc
    29101 tggaagaaaa aaatagtcaa tctcaccgtg ccttagccgt taagaaactt ttggaggtat
    29161 ttccatcatg gcaaagcaaa ccatcattgt aatgagcgat tcccatggcg atagcttgat
    29221 tgtggaagaa gtccgtgatc gctatgtggg caaagtcgat gccgtttttc ataacggcga
    29281 ttctgaacta cgtccggatt ctccactttg ggagggcatc cgcgttgtta aagggaacat
    29341 ggacttctac gccggctacc cagaacgtct ggtgactgag cttggttcga ccaagattat
    29401 ccaaactcat ggtcacttgt ttgacatcaa tttcaacttt caaaagttgg actactgggc
    29461 tcaggaggaa gaggccgcta tctgcctcta tggtcacttg catgtgccaa gtgcttggat
    29521 ggaaggcaag atcctctttc taaatccagg ttctatcagt caaccacgag gtaccatcag
    29581 agaatgtctc tatgctcgtg tggagattga tgatagttac ttcaaagtgg actttttgac
    29641 acgagatcac gaggtgtatc caggtttgtc caaggagttt agccgatgat tgccaaggag
    29701 tttgagactt tcttgttggg gcaggaggaa acttttttga cccctgctaa aaatctagct
    29761 gtgttgattg atacccacaa tgcggatcat gcgaccctct tgctcagtca gatgacctat
    29821 acccgtgttc ccgttgtgac agatgaaaaa cagtttgttg ggacgattgg actcagagat
    29881 attatggctt atcagatgga gcatgacttg agccaagaaa tcatggcgga tacggatatc
    29941 gttcatatga caaaaacgga cgtagcggtt gtttcgcctg atttcaccat tacggaggtc
    30001 ttgcacaagc tagtagatga gtccttctta ccggttgtgg atgcagaggg tattttccaa
    30061 gggattatta cgcgcaagtc catcctcaag gccgttaatg ccctcttgca tgactttagt
    30121 aaggaatatg atattcgatg ccaatgagag acaggatttc agccttttta gaggaaaagc
    30181 agggcttgtc tgtcaattcc aagcagtcct ataagtatga tttggagcaa tttttagaca
    30241 tggtaggtga gcggatttct gagaccagtc tcaagattta ccaagcccag ctagccaatc
    30301 taaaaatcag cgcccagaag cgaaagattt cggcctgtaa ccaatttcta tactttctct
    30361 atcaaaaagg agaggtggac agcttttacc gcttggaatt agccaaacaa gctgaaaaga
    30421 agacggaaaa gccagagatt ctatacctag actctttttg gcaggaaagc gaccatccag
    30481 agggccgctt gctagcgctc ttaatcctag aaatggggct cttgcccagt gagattttag
    30541 ccatcaaggt tgcggacatc aatctggatt ttcaggtgtt gcgaatcagc aaggcttccc
    30601 aacagaggat tgtcaccatt cccacggcct tgctttcaga attggaaccc ttgatggggc
    30661 agacctatct ttttgaaaga ggagagaaac cctattctcg tcagtgggcc tttcgtcagt
    30721 tagaatcttt tgtcaaggag aaaggttttc catccttatc agctcaagtc ttacgtgaac
    30781 agtttattct aagacaaata gaaaacaagg tcgatttgta cgaaattgca aaaaaattag
    30841 gattaaaaac agtcctgacc ttagaaaaat atagataatg gatattaaat taaaagattt
    30901 tgaaggaccc ctggacttgc tcttgcatct ggtttctaag taccagatgg atatctacga
    30961 tgtgcccatt acggaagtca tcgaacagta tctagcctat gtctcaaccc tgcaggccat
    31021 gcgtctggaa gtgacgggtg agtacatggt catggctagt cagctcatgc tgattaagag
    31081 tcgtaaactc cttccgaagg tagcagaagt gacagacttg ggggatgacc tggagcagga
    31141 cctcctctct caaatcgaag aatatcgcaa gttcaagctc ttgggtgagc acttggaagc
    31201 caagcaccaa gaacgggccc agtattattc caaagcgccg acagagttga tttacgaaga
    31261 tgcggagctt gtgcatgaca agacgaccat tgacctcttt ttggcttttt caaatatcct
    31321 agccaagaaa aaagaggagt ttgcacaaaa tcacacgact atcttgcggg atgagtataa
    31381 gattgaggac atgatgatta tcgtgaaaga gtccttgatt ggacgagatc aattgcgctt
    31441 gcaggatttg ttcaaggaag cccagaatgt ccaagaggtc atcaccctct ttttggcaac
    31501 cctagagtta atcaaaaccc aggagctgat cctcgtgcaa gaggagagtt tcggagatat
    31561 ctatctcatg gaaaagaagg aagaaagtca agtgcctcaa agctagactt gatagagagg
    31621 aaagatgagt actttagcaa aaatagaagc gctcttgttt gtagcgggtg aagatgggat
    31681 tcgggtccgc cagttagctg aactcctctc tctgccaccg acaggcatcc agcaaagttt
    31741 aggaaaatta gcccagaagt atgaaaagga cccagattcc agtttggctt tgattgagac
    31801 aagtggtgct tatagattgg tgaccaagcc tcaatttgca gagattttga aggaatactc
    31861 taaggcgcct atcaaccaga gcttgtctcg ggctgccctt gagaccttgt ccattattgc
    31921 ctacaaacag ccgattacgc ggatagaaat tgatgccatc cgtggagtta actcgagtgg
    31981 agccttggca aagttgcagg cttttgacct gataaaggaa gacgggaaaa aggaagtatt
    32041 ggggcgcccc aacctctatg tgactacgga ttatttccta gattacatgg ggataaacca
    32101 tttagaagaa ttaccagtga ttgatgagct tgagattcaa gcccaagaaa gccaattatt
    32161 tggtgaaagg atagaagaag atgagaatca ataagtatat tgcccacgca ggtgtggcca
    32221 gtaggagaaa agcagaagag ctgatcaagc aaggcttggt gacggttaac ggccaagtgg
    32281 tgcgtgaact agcaaccact atcaagtcag gcgacaaggt cgaagttgaa ggtcaaccta
    32341 tctacaacga agaaaaggtc tactatctgc ttaacaaacc acgcggtgtc atttccagtg
    32401 tgacagatga taagggtcgc aagacggttg tcgacctctt gcccaatgtc aaagagcgta
    32461 tttaccctgt gggtcgtttg gactgggata catcaggtgt cttgattttg accaatgatg
    32521 gtgactttac agacgagatg attcaccctc gtaatgagat tgacaaggtt tatgtcgcgc
    32581 gtgttaaagg tgtggccaat aaggacaatc tccgcccctt gacccgtggt cttgagattg
    32641 atggtaagaa aaccaagcca gctgtttatg aaattctcaa agtggaccca gtcaaaaatc
    32701 gctctgtggt gcagttgacc atccatgaag ggcgtaacca tcaggttaaa aagatgtttg
    32761 aagctgttgg tctccaagta gataagttgt ctcggactcg tttcggacac ctagacttga
    32821 caggactccg tccaggagaa tcccgtcgtc ttaataaaaa agaaatcagc caactacaca
    32881 ccatggctgt aactaagaaa taatgaaacg aattttaata gcgcctgtgc gcttttacca
    32941 acgttttatc tcaccagtct ttccaccctc ttgtcgcttt gagctgactt gttccaacta
    33001 catgattcag gctattgaaa aacatggttt taagggggta ttgatgggct tggctcggat
    33061 tttacgttgt catccctggt cgaaaacagg taaggacccc gtttcagacc acttttccct
    33121 taaacgaaat caagaagggg aatgaggtgg ggtaaataga tttcaaaatg ataaaaacgc
    33181 atcctatcag gtttgagtaa acttgatagg atgcgtttta gaatgtcaaa attttatact
    33241 cttcgaaaat ctcttcaaac cgcgtcagct ttcatctgca acctcaaaac agtgttttga
    33301 gcaacctgcg gctagtttcc tagtttgctc tttgattttc attgagtatt aaattgagtt
    33361 tgaagtggct tatttcaaag ctttttgtat gtcttcaatc atgagttttg ttgattcaag
    33421 tccgcctccg cttagatacc agaggtctgg tgttagttgg ataatcttac catttttagc
    33481 agcaggtgtt tcagcgataa gggcattttc taggacaccg tcgttgctag agttgtcccc
    33541 accgatggca agggtacggt tgatgacaaa gaggatgtca gggttgattt ctttgacact
    33601 ttcaaagctg acttcttgtc cgtggcgtga gtcttcaaat tttgtatcag ttggtttgaa
    33661 tttcaaggtt tggtacaaga aagagaaacg agatttggca ccaaaggctg ccatttttcc
    33721 ttcattaagg aggatcgcaa gggctttttt gtcagagctt tcatttttag tagcgacttc
    33781 ttggatgctc ttgtctagct tggccaattc ttccttggct ttttgtgtac cagtttcgcc
    33841 gaaggcactt gctaaggatt cgatattagc cttggtagaa gtccagtagt cgtccttgct
    33901 tgcttggaag agaacggttg gggcgatttc tttgaatttg tctacgaatt tttgtgtacg
    33961 tggtgaagcg ataatcaaat caggctcaag ggcggcgata gcttctaaat caggttcttt
    34021 catagaacca acatttttga cagttcccac taggtctttt agataagtcg gaacagtttt
    34081 tgtaggcatt ccgacgatat ttttttcaaa tcctaaagcg cgaatagtat ccgcagcgcc
    34141 gaggtcaaag gtcacaatct tttcaggaac tttggaaagt ttgacctcgt ccagtgaact
    34201 tttaatggtt atctctgttg gagcagagct actggtctct gtctgactag tgcttgagtt
    34261 tgtactacat gcaccaagta ggagcaagaa gctggccact agggcagtga aataaagttt
    34321 aagggatgtt ttcataattt ctccttttta aaatgtgata acgatttagg gagtctctta
    34381 atcttattga ctaagagact gaaggttctc taacttgagc ttttatgtta ctagctatag
    34441 atacagatct ttttgtcatt gatatcagct agcgtgatgg gaatctcata aagttgactg
    34501 agcaggtcag cctgcatgat ttgatcggtt cttcccttgc taaagacctg gccgtccttg
    34561 aaggcgacaa tttcatctgc atactgactg gccatgttga tatcgtggag gacgatgata
    34621 atggtcttgc cgagttcctc caccagtcgt cgaagaatct gcatcatgct gacgctttgc
    34681 ttgatatcga gattgttgag tggttcgtcc agcaagataa agtccgtatc ctgggccagt
    34741 accatagcga taaagacgcg ctggagttgc ccccctgaca ggctattgat gtagcggtct
    34801 tttaagttgg tcagttccaa atagttcaga gtttctcgga ttttttccca gtcttctgat
    34861 ctaagtcgac ctcggctgta gggaaaacgt ccaaaactga ccagttcttc aacagtcaat
    34921 ttggcttggt aattgatttt ctgttttagg atggttagtt cttgggccag ttcttgcgaa
    34981 ttccagctct cgatttcacg tcctttgata ctgagaactc cctgatcttt cttggttagt
    35041 ctgctcatga tggagaggag agtcgatttt ccagcaccat ttggaccaat aaaggctgtc
    35101 agtttttgag gactgacttc aagcgaaatg ccttgcaaaa tatcctgttt ttgaatggat
    35161 ttgtcaatgt tttccagttt cactgacgag acctcctata tagtaagata aagaataaga
    35221 agccacccac actctcaatg atcatactga tacgaatttc cagtgcaaag actcgttcaa
    35281 tcaagacttg ccccaaggtt aagctaataa atccaaccag aatggccact ataaagagta
    35341 acttgtgctg atagtctttg acaatcaggt aggtgaggtt ggccagcata aagccgaaga
    35401 aggccatagg tcctaccaag gcagtggccg ttgaggtcaa aagcacgatt ccccagagga
    35461 gctctttctg ttctttttca acatcgagtc ccaatatctg agccgtttct ctttgcaggt
    35521 gcaagacatc tagaacgact gcttttcgaa agaaaaagat tgtcaaagcg aggatgatca
    35581 gagaaccgat ggctaggatg gaagtgttga gatgttgaaa ggaggcaaaa agactatttt
    35641 gcagtttatc gtattcgttt ggatccatta ggacttgaag gaaggtgcta atatttcgaa
    35701 agagacttct gagcgctaga cagatcagca ggatgaagac caggtcttgc ttcatcagtg
    35761 tcttcaagta accttgtaag gcgagaaaga agaaggactg gacaagaagt aagactagga
    35821 attctaagat aggggatttg ccaagttgaa gaaacttgct ttcaaaaacc agtagtaggg
    35881 tttgtagtag gacgtagaag gattcaattc ccaaaatact aggcgtcagg aagcgatttt
    35941 ccgtcagggt ttgaaaacta atggtcgaaa tcccagtcgc gatggctacc aagagataaa
    36001 cgatgatctt ttgggaacgc aacttccaag caaaggctga caagtgagtg atgggccaaa
    36061 agtagagaag acaagctccg atggcaagaa taatgagaat ccagaagagc ttggtatgtt
    36121 tgcttttagt ctgcatcttt tcgtccccct ctccagagaa gtaggataaa gacgagacta
    36181 ccgatgattc ctagcaagag actgacagac aactcatagg acctaatcag aactcgggat
    36241 aggatatcgc aagccagaac tagattagca ccaaccagtg cgaccatgag tttggtttga
    36301 cttagattat ctccatagcg cttgcgaaca agattgggaa cgataactcc gagaaatggt
    36361 aggccaccca cggtaatcat ggtgacgctt gtcgttagcg ccaccagaaa gagggtcagt
    36421 ttttcaagta gggagtagga aatccccaaa ctctcgctgg tttctttccc tagattcatg
    36481 atggtgaagg tttgggataa tttccaaacg gttatcagga tgatgaggcc taagaagagc
    36541 cactcatact gatgggtctg aatcatggag aaggagccct gggtccaggc agtcatactc
    36601 tgaaccagat tgaaacgata ggcgataact tctgtgactg agccgataat cccgctatag
    36661 atgatcccaa tcagaggcaa catccacctt tcctttacag taaaaatggt cataaaggct
    36721 agaaagaaga gggtgaatac gatggatgaa acaaaagcga agagcatctt ttgggtcaga
    36781 ctagccgatg gaaagacaaa aaggctcagc accattccca gtttggcggc ttcagtcgtt
    36841 ccaactgtac tcggtgcagc aaactgattt tgggtaatag tctgcatgag aaggcctgcc
    36901 atactcatac tagaggcagt caggagaata ctgatagttc ttgggagacg ggactcttga
    36961 aagaggagcc aggtctgctg gtcgaaatca aatagctttc cccatgaaaa atcactggtc
    37021 ccaatgctaa tagagagaaa gactaggagt agaagtaagc caattaaata atgagaaagt
    37081 ttcatacccc gtcccttcat gtagatttgg tatcgaaaga tacctgcgga tataaatgta
    37141 acattatttt tctaatctgt caataaaatt tctgacaatt taataaatac aacaaggaga
    37201 gagcaacaaa actttctcct ttgttatcct attctaaaat gtttttacct taatctgata
    37261 aaataatatc ttcgagggag tagctagccg tccaatcaag atattgttta gcttttgaag
    37321 catctgctag gacactggct gggtcactag cacgtcgagc aacaatctcg tgtgggattt
    37381 tttaatttag taattcttca gcagttttaa agatttcttt gatagtatag ccttttttag
    37441 ttcctaagtt aaagatttga gaagaactgt cttcttaaaa taggtagttc attcctttaa
    37501 catgagccta tgcaaggtcc aagacataaa tgtaatctcg aatacatgaa ccgtcacgtg
    37561 tatcgtagtc atctccaaat atttttaagc tatcattttg tcccaatgcg gtcttgttga
    37621 tatttggaat gatgtgagtt ggatttttca cacgcagacc gtttgaagta tccatttcag
    37681 ctccagcaac attaaagtaa cggaaaataa catatttcca gtcgtagcga ttggccatcc
    37741 agtaaatcat tcgttcgccc atcagttttg tctctgcata agggttgaca gggtcgagca
    37801 gggtatcttc agtcaccggc ttgtcaatac agttatttcc atagagagaa gcagtcgaag
    37861 agaacatgat tttttgaatg ccaacttcag ataagacttt gagaacttgg ttcataccag
    37921 caacgttggc agtgaagtat ttacttggat tttcaatact ttcgctcaca acaatttcac
    37981 ctgcacaatg gaggacagca tcaatctgat tttcttctaa gtaagccttt aaggcgctag
    38041 catcataaac atccagttct ttaaagctag cacgactatc cacagccgct cgatttcctg
    38101 tggagagatt atcgaggaca tgcaccttat agccagcatt taaaagagct ttaacggtat
    38161 gggagccaat gtagctagtc ccacctgtaa caagaattgt tttggtcatg attttttcct
    38221 tttctagttt tgtactatag taaattgaaa taagatatga acaactctat taggaaagtc
    38281 aaattaattt ctagaaatgt ttcagcagct acagcgtact attccaaact caaccaacta
    38341 tatatttaaa gaaagaagaa agggaagtca actattttct gtaaatttca tgaaaaatcc
    38401 aaacaacttt gatggtgtat tgtttttttg tttggaaata aatattcgag ttaattttgg
    38461 aatgtttact agattttgca ctacatgcaa gagaatacta tccttttcta agatagagca
    38521 ggactaagat actaacttag aaatccacct tcccactatc cttctcctat aaaaagtggt
    38581 aaaatggatg aaagaaagaa ggaactgaaa tgacaacatt attttcaaaa attaaagaag
    38641 taacagaact tgctgcagtc tcaggtcatg aagcgcctgt ccgtgcttat cttcgtgaaa
    38701 agttgacacc gcatgtggat gaagtggtga cagatggctt gggtggtatt tttggtatca
    38761 aacattcaga agctgtggat gcaccgcgcg tcttggtcgc ttctcatatg gacgaagttg
    38821 gttttatggt cagcgaaatc aagccagatg gtaccttccg tgtcgtagaa atcggtggct
    38881 ggaaccccat ggtggttagc agccaacgtt tcaaactctt gactcgtgat ggtcatgaaa
    38941 ttcctgtgat ttcaggttct gttcctccgc atttgactcg tggaaagggg ggaccaacca
    39001 tgccagccat tgccgatatc gtttttgatg gtggttttgc ggacaaggct gaggcagaaa
    39061 gttttggcat ccgtcctggt gataccatcg taccagatag ttctgcaatt ttgacagcca
    39121 atgaaaaaaa tatcatctca aaagcttggg ataaccgcta cggtgtcctc atggtaagcg
    39181 agctagctga agctttatca ggtcaaaaac tcggcaatga actctatctg ggttctaacg
    39241 tccaagaaga agttggtttg cgtggcgctc atacctctac aaccaagttt gacccagaag
    39301 tcttcctcgc agttgattgc tcaccagcag gtgatgtcta cggtggtcaa ggcaagattg
    39361 gagatggaac cttgattcgt ttctatgatc caggtcactt gcttctccca gggatgaagg
    39421 atttcctttt gacaacggct gaagaagctg gtatcaagta ccaatactac tgtggtaaag
    39481 gcggaacaga tgcaggtgca gctcatctga aaaatggtgg tgtcccatca acaactatcg
    39541 gtgtctgcgc tcgttatatc cattctcacc aaaccctcta tgcaatggat gacttcctag
    39601 aagcgcaagc tttcttacaa gccttggtga agaaattgga tcgttcaacg gttgatttga
    39661 ttaaacatta ttaaaccata agggaggtgg tagggatggt agaaccaaac ctagaaagcc
    39721 ttataaaaga tctttacaat catgctcgac atgatttgag tgaagattta gttgctgctc
    39781 tcctagagac tactaaaaaa ctgcctacta caaatgagca attgcaggca gttcgtctct
    39841 caagcctggt caatcgtgaa ttgctcctaa atcccaaaca tccagcacct gagttgctca
    39901 acttggctcg ctttgtcaaa agagaagaag ccaagtacag aggaactgcg acttctgcgc
    39961 ttatgtatga ggaacttttt aaaatgcttt gattccattg tggatcaaag cattttttat
    40021 aagacctatc tcgtgagcgt tcaaatagta gtaacaagga atcatgagaa gtcagccgag
    40081 accatagtag tgaagaaact tctgtaatgg aagtggagtg aagtgaggaa tcgtcaaacg
    40141 gtaggggata ggctgtttga agggtgctac gaaaaagata gggtaaggtg aaattatagc
    40201 ggaatgaaat aagatatgaa caaattgatt agggaaatca aatgaattta tagaaatctt
    40261 ttagcagtca tggcgtccta ttctagtttc aaaatgctat aaatacgaac aaattgattg
    40321 ggaaattcaa atcgctttct atcaatgttt tagaagatac agtgccctag tccaacttca
    40381 atatactata aaagaacaga tggaagcaat tatgatttac ttctatcttt aaaaaaaata
    40441 aatgatctga taggaaaaat gtgtcccctc ataaaaaata ggatagtacg gatggaaggc
    40501 ttttgtagac tggaggacga aggaatttag aaactctcgt taaaagttag ttgagccgga
    40561 gtatggtggt gtgaataatt atcaaaaatt atttccaaat cgaaatagtt gacagttaga
    40621 acgatgagtg ttacaattat tttattcatt tcgatatcga aataaattgg aggtgttatg
    40681 aaagatagtc atttgctagc ccatcatatt cgtttgttga atgggcggat ttttcaaaag
    40741 ttactgagtc aagatcctga ggctctttat aggggtgaac agggcaagat tttagcggtt
    40801 ttatggaata gtgaaactgg ctgcgcaact gcgacagata tcgcgcttgc gactgggctt
    40861 gcgaataata cgctgacgac tatgataaaa aagctagagg aacaaaagct tgtaattgtt
    40921 agtccgtgtg gaaaagacaa gcgtaagaag tatttagttt taacggagtt aggcaagttc
    40981 cagaaagaag tggggcatcg tgtcagtcag aaattggata ctatctttta caaaggattt
    41041 tcagaggaag aaattcacca atttgaaggt tttcaagaaa gaattttggc aaatctgaaa
    41101 gagaaggaaa atgaggttta gaatatgtca caacaagtga aaaatgctca caacctatac
    41161 atccatgcca ttcaagacgg acgagttgct gaggctcaag cccagtctgt aggggatacc
    41221 tatatccaac attcgacagg tgttcctgac gggaaagaag gatttgcagc cttctttgca
    41281 gatttctttg agcgtcatcc agagcgtcag attaagattg tccgcaccat tgaggatggc
    41341 aatctggtct ttgttcatgt tcatcaatat ctgaatggtg gagaagctca atgggtgacg
    41401 acggatactt tccgtgcgga tgagaatggt cgtattgttg agcattggga tgtcattgac
    41461 tactatcgaa cacctgaaaa tgaccaacta gatcaaattt ttggagattt tgaaatcaag
    41521 gatttggaca agaaagcaga aaataaaaag ctggttcgcc gtttcttgac agaaactttc
    41581 caaaatgggg agctagagca gtggagtgat tatgtggcag acgatttgat tcagcataat
    41641 catgagattg gacaaggaag tgctgcttat aaaaactatg tggctgaata tagtgtcact
    41701 tttgactttg ttttccaact cttaggacaa ggaaactatg tggttagcta tggtcagact
    41761 cagattgatg gcgttgctta tgcccagtac gatatcttcc gtttagagaa cgggaaaatt
    41821 gtggagcatt gggataataa ggaagtcata cctaaggtag aagacttgac taatcgaggg
    41881 aagttttaaa ttgaggacaa agaatgattg aatacaaaaa tgtagcactg cgctacacag
    41941 aaaaggatgt cttgagagat gtcaacttac agattgagga tggggaattt atggttttag
    42001 tagggccttc tgggtcaggt aagacgacca tgctcaagat gattaaccgt cttttggaac
    42061 caactgatgg aaatatttat atggatggga agcgcatcaa agactatgat gagcgtgaac
    42121 ttcgtctttc tactggttat gttttacagg ctattgctct ttttccaaat ctaacagttg
    42181 cggaaaatat tgctctcatt cctgaaatga aggggtggag caaggaagaa attacgaaga
    42241 aaacagaaga gcttttggct aaggttggtt taccagtagc tgagtatggg catcgcttac
    42301 ctagtgaatt atctggtgga gaacagcaac gggtcggtat tgtccgagct atgattggtc
    42361 agcccaagat tttcctcatg gatgaaccct tttcggcctt ggatgctatt tcgagaaaac
    42421 agttgcaggt tctgacaaaa gaattgcata aagagtttgg gatgacaacg atttttgtaa
    42481 cccatgatac ggatgaagcc ttgaagttgg cggaccgtat tgctgtcttg caggatggag
    42541 aaattcgcca ggtagcgaat cccgagacaa ttttaaaagc gcctgcaaca gactttgtag
    42601 cagacttgtt tggaggtagt gttcatgact aatttaattg caacttttca ggatcgtttt
    42661 agtgattggt tgacagctct atctcaacat ttgcagttgt cacttttgac cttgttacta
    42721 gctattttgc ttgcgattcc cttggctgtt tttcttcgct atcatgagaa gctggccgac
    42781 tgggtcttgc agattgcagg tattttccag accatcccgt ctctggcctt gttgggactc
    42841 tttattccct tgatgggaat tgggacttta ccggcattga cagctctagt gatttatgcg
    42901 atttttccga ttttacaaaa tactattact gggctgaagg gaattgatcc gagtcttcaa
    42961 gaggctggga ttgcctttgg gatgaccaga tgggagcgtc tcaagaagtt tgaaattcca
    43021 cttgccatgc ctgttatcat gtctggaatt cggacggcag cggtcttgat tatcggtacg
    43081 gctaccttgg cgaccttgat tggggcaggg ggactgggtt cctttatcct gttgggaatt
    43141 gaccgtaata atgccagttt gattttgatt ggggcccttt cttctgcagt gctagctatt
    43201 gcctttaact tcctactaaa agtaatggaa aaagcgaaat tgcggacgat tttctctggt
    43261 tttgccttgg tggctttatt actgggtctg tcttatagtc cagccctttt ggctcaaaaa
    43321 gagaaggaaa acttggttat tgctgggaaa ataggcccag aaccagaaat tttggctaat
    43381 atgtataaat tgctgattga agaaaatacc agcatgactg cgactattaa accgaatttt
    43441 gggacgacaa gtttccttta tgaagctctg aaaaaaggtg atattgacat ttatcctgaa
    43501 tttaccggta cggtgactga aagtttgctt caaccatcac ccaaggtgag tcatgagcca
    43561 gagcaggttt atcaagtggc gcgtgatgga attgccaagc aggatcatct agcctatctc
    43621 aaacctatgt cttatcaaaa tacctatgct gtagctgttc cgaaaaagat tgctcaagaa
    43681 tatggcttga agaccatttc ggatttgaaa aaagtggaag ggcagctgaa ggcaggcttt
    43741 acgcttgagt ttaacgaccg tgaagatgga aataagggct tgcaatcaat gtatggtctc
    43801 aatctcaatg tggcgactat gcagccagct cttcgttatc aggcaattca gtcaggggat
    43861 attcaaatca cggatgccta ttcgactgat gcggaattgg agcgttatga tttacaggtc
    43921 ttggaagatg acaagcaact cttcccacct tatcaagggg ctccactcat gaaagaagct
    43981 cttctcaaga aacacccaga gttggaaaga gttcttaata cattggctgg taagattaca
    44041 gaaagccaga tgagccagct caactaccaa gtcggtgttg aaggcaagtc agcaaagcaa
    44101 gtagccaagg agtttctcca agaacaaggt ttgttgaaga aatgatggga cagagacttt
    44161 aaacatactg aactcaactt ccttaaacga ctagataaaa gaatgctcag acaatttcgt
    44221 ctgggctttt ttgatgtgag aatgactgaa tgtcacttaa aatggaaaga aagagaaaat
    44281 tttcatgatt ttctaaaatt ttactgtaaa gacagttgac tattcataaa ataggtgtat
    44341 aatattttgt gaactactta acaaataaga ggtttcagct catgtcgact aaggttagaa
    44401 atcttgtgta taggcagttc attatctaat agagcgttgc gtccgaaagt ctatccagac
    44461 acggctcttt aaaaacaaaa ggagaaatga tgcatactta tttgcaaaag aaaattgaaa
    44521 atatcaaaac aaccctaggt gaaatgtcag gtggttaccg tcgtatggtt gcggctatgg
    44581 ctgatttagg attttcagga actatgaagg ctatctggga tgacctcttt gcccatcgta
    44641 gttttgccca gtggatttat ttgctggttt taggaagttt tcctctctgg ctggagttgg
    44701 tttacgaaca tcgtattgtt gactgtattg ggatgatttg tagcttgaca gggattatct
    44761 gtgtaatctt tgtatcggaa ggtcgagcaa gtaattatct ttttggcttg attaactctg
    44821 ttatttacct tattttggcc ctacagaaag gcttttatgg tgaggtgctg acgacacttt
    44881 acttcacagt catgcagcca attggacttc tagtttggat ttatcaggca cagtttaaga
    44941 aggaaaagca ggagtttgtc gcacgtaaac tggacggcaa gggctggaca aagtatcttt
    45001 ccattagtgt gctttggtgg ttggcctttg gcttcattta tcagtctatt ggtgccaatc
    45061 gtccctatcg tgattcaatc acagatgcaa ccaatggggt agggcaaatc ctcatgacag
    45121 ctgtttaccg tgaacagtgg atattctggg cggctaccaa tgtcttttca atctatctct
    45181 ggtggggaga aagcctgcaa attcaaggga aatatctaat ttatctcatt aacagtctag
    45241 ttggttggta tcaatggagc aaggcagcta agcagaatac tgatttactt aactaggaaa
    45301 agatgtttga aagtgctgtt ttgagatttc gattaaaaca gatatagttg ataatcaagg
    45361 atttatagta tgaaaaagag gatcggcggg tcctcttttg ttgttgaaaa gataaaaaac
    45421 tcagtaacct agaaataaga caactgaagc tttactctat attcaatttt taggaatgag
    45481 aaggtctaga taaaattgga caacttcctg gtctgtgaaa tcttgacctt ttttgagcca
    45541 ccaggtcaat gtttcgataa agttagttac gacaaaatgt tggaggtaag aagtaggcag
    45601 attagggtgg gcttctttta aattatcagc tagcacggaa tagacatggt gttctagctc
    45661 tttatggagt tgacggagga agtagtcatt tttggaaaat agcagactgg tgatatggtc
    45721 ttggttttta tgaaaatgca gaaagaggtg ggcgaggtag tcctcggttg aaatggcttg
    45781 ctctctttca aaaagatgat ggaagaggta gcggcagagc tggtccagaa gaagctcctt
    45841 actctcatag tgacagtaaa aggtggatcg tcccacatct gcgagatcaa tgatatcctg
    45901 aacagtagtg gcctcgtagc ccttagcatt caaaagttgt ataaaagctt gatagatggc
    45961 ttttttggtt ttgctgatac ggcggtcaat gttagtcata tggacacttg aggcaaactg
    46021 ttcagaactg aataaagctg acgttttgct tctatccttt ctttgagttt tagtggataa
    46081 tgataatgaa caagatgttc ataaatctat tataacaaag gaatgagaaa tatgaaggca
    46141 aaatatgctg tttgggtggc ttttttctta aatttgactt atgccattgt tgagtttatt
    46201 gcaggtggag tatttggttc tagcgctgtt cttgctgact ctgtgcatga cttgggagat
    46261 gcgattgcaa ttggaatatc agcttttcta gaaacaatct ccaatcgtga agaagacaat
    46321 cagtacacct tgggctataa gcggtttagc ctgctaggag ccttggtaac agctgtgatt
    46381 ctcgtaacgg gctctgttct agtcattttg gaaaatgtca cgaagatttt gcatccgcaa
    46441 ccagtcaatg atgaggggat tctctggtta ggaattattg cgattactat caatctgtta
    46501 gcgagtctgg tggttggtaa gggaaagaca aagaatgagt ctattctgag tctgcatttt
    46561 ctggaagata cgctagggtg ggtagctgtt atcctgatgg cgattgttct tcgatttacg
    46621 gactggtata tcctagatcc tcttttgtcc cttgtcattt ctttctttat tctttcaaaa
    46681 gcccttccac gtttttggtc tacactcaag attttcttgg atgctgtgcc agaaggtctt
    46741 gatatcaagc aagtaaagag tggcctggag cgattggaca atgtggtcag ccttaatcag
    46801 cttaatctct ggactatgga tgctttggaa aaaaatgcca ttgtccatgt ttgtctaaaa
    46861 gaaatggaac atatggaaac ttgtaaagag tctattcgaa ttttcctaaa agattgtggt
    46921 tttcaaaata ttaccattga aattgatgct gacctagaaa ctcaccaaac ccataagcga
    46981 aaggtgtgtg acttggaacg gagttatgag catcaacatt agaaaaaagt gaaaaatact
    47041 tgggtactat cttatttgga atagagtaat ttctttatta tttaaatatt tcaaaaattg
    47101 gtaagagaag agcattgtat aaactccaga tatatgattg ttaatgataa aaatttttcg
    47161 attagataca aaatgcttaa cttggagtca actcaaagtt atataataag ataagtgagt
    47221 tagaatagcg tgaattcagt gaatgaaatg agaggaggtt agcgtgtgaa tattaaatct
    47281 gccagtgatt tgttgggaat ttcagcggat acgattcggt attatgaacg ggttggtctt
    47341 gtgccaccga ttactcgtac tgctactggg attcgtgatt ttcaagatca ggatatcgaa
    47401 gcgctggaat ttattaagtg ttttcgttcg gcgggtgtct ctgtagatag tttagttgac
    47461 tatatgtcgc tctaccaaaa gggagatgaa acgagagagg agaggcttgg tattttagaa
    47521 gaggaaaagc aaaaattaga ggagcgcttg tctcagctac agacagcttt aaatcgttta
    47581 aatctcaaaa ttaaacttta taaggaagga aaattttaaa tgaaatcagc agtatataca
    47641 aaggcaggtc aggttggact tgctagcatt gaacgtccgc aaataataga agcggatgat
    47701 gtgattattc gtgtggttcg tgcgtgcgtt tgtggttcag atttatggag gtaccgtaat
    47761 ccagaaacga aagctggaca taaaaatagt ggacacgaag cgattgggat tgttgaagaa
    47821 gctggggaag ccattacgac ggtgaaacca ggtgattttg tgattgtccc ttttacacat
    47881 ggatgtggtg agtgtgatgc ctgtcttgct ggatttgacg gttcttgcga caatcatatt
    47941 ggcaataatt tggggggtga ttttcaggca gaatatattc gcttccacta tgcaaactgg
    48001 gcgctggtta aaatccctgg tcaaccttct gactatacag aagggatgct caagtccctt
    48061 ttgactcttg cagatgtcat gccgacaggc tatcatgcag cgcgtgttgc aaatgttcaa
    48121 aaaggggaca aggttgttgt tatcggtgat ggggctgttg gtcaatgtgc tgtcatcgcg
    48181 gctaagatgc gtggagcatc acaaattatc cttatgagcc gtcatgaaga ccgtcaaaag
    48241 atggctatgg agtcaggtgc gacagctgtt gttgcagaac gtggtcaaga aggaattacc
    48301 aaggtgcgtg aaatcctcgg tggaggagca gatgcagcac ttgaatgtgt tggtacggag
    48361 gctgctatag aacaggcgct aggtgttctt cataatggag ggcgtatggg ctttgtagga
    48421 gtcccacact ataataatcg tgctcttggt tcgacattta tgcaaaatat ctctgtagca
    48481 ggtggggcag cttctgctac aacatacgat aagcaatttt tactaaaagc cgtccttgat
    48541 ggtgatatca atccaggtcg cgtctttact tcaagttata aactggaaga tatcgaccaa
    48601 gcctataaag atatggatga acgtaagaca attaagtcta tgattgtaat cgaataaaaa
    48661 acgaatagga gttttagaac tctattcgtt ttttatgtta tcctattctt gatttagggt
    48721 attttctctt aatgtcagtc tggttcccag catggtcagg ctaggaattt tgcgaccgtg
    48781 gaggacttcc ttgttaagaa tatccatacc tgctcgcccc atttcttcag tataaactgt
    48841 gatgctggag aggggaggat aaacctgctt ggtcaggctg gtgtcgttaa aggaaatgag
    48901 gctgacacgg tctggtaggc tgattccagc ttcttggagc gcacgaaggg caccgatggc
    48961 taaactatcg ctggctgcga aaaatgctgg tgggagttgg tctcccaagc tctgaatggc
    49021 ttccttcatc aagtcatagc cagactgggc agtaaagttt ccttgaaaga ccagttcatc
    49081 atgatagatt ccttttgctt gactgtagtt tctgaaattt tccagccgct tatcctgaat
    49141 gatttcttct tggtcagtcg tttcttcaag gcctgttaga atcccgatac ggtccattcc
    49201 ttggctgagg aaataatcga caacctgttt catagcagtg taaaaatccg tgataataca
    49261 ggtatgtccc agggaaagtg tatcactgtc tagaaagacc aggggttttt ggtattcttc
    49321 aaaggcagaa atctgagctc ggctaaattt tccaatgcag agaatcccaa tcacttcctc
    49381 gcttaaggta aaaggatggt cattaaaata gcgcaagata tcatagtcca actcttgggc
    49441 tcttttttct attcctaggc gaatctggta gtagtagagg tcgtccagct ccccttgttc
    49501 gctgacccat tggataatgg caatcttttg cttgggttta tgggactcgc ctgtcttgag
    49561 gtgcttggtg tagcccagct cttcagcaac ggttaaaata cggtgtctgg tttcttctgt
    49621 aacagatagg ctctggtcgc ggttgaggac acgggatacg gtcgcgatag agacagaggc
    49681 tagctgtgca atgtctttta aggtagccat aaatcctcct tgattaggtt agtatatcat
    49741 atttttctgc tttttactga tattttagta aaattttagt aaaaaggatt gaccttggga
    49801 aatcccttgg atacaataga agaaaacgat tacacgttaa ggtggcttaa cggacagtca
    49861 aaggagaaat catatgacac aacatcttac tgctgaaact cttcgcaaag actttcttgc
    49921 tgtttttggt caagaagcag accaaacttt cttttcacca ggtcgtatca atttgattgg
    49981 tgaacacaca gactacaacg gtgggcacgt ttttcctgct gctatttcct tgggaactta
    50041 cggtgcagct cgtaagcgtg acgaccaagt tttgcgtttc tactcagcta actttgagga
    50101 caagggcatt atcgaagtgc ctctcgctga cctcaagttt gaaaaagagc acaactggac
    50161 caattatcca aaaggtgtcc ttcatttctt gcaagaagct gggcacgtga ttgacaaagg
    50221 ttttgatttt tatgtttatg ggaatatacc aaatggtgct ggtttgtctt cttcagcatc
    50281 cttggagctc ttgacaggag tcgtggcaga gcatctcttt gatttaaaat tagagcgtct
    50341 cgatttggtt aaaatcggaa aacaaacaga aaacaacttt atcggagtca actctggtat
    50401 catggaccag tttgctattg gtatgggggc tgatcaacgt gctatttacc ttgataccaa
    50461 caccttagag tatgatttgg tgccacttga tttgaaggac aatgtcgttg ttatcatgaa
    50521 caccaacaaa cgccgtgaat tggcggactc taaatacaat gaacgtcgtg ctgagtgtga
    50581 aaaagcagtg gaagaattgc aagttgcctt ggatattcag accctgggtg aattggacga
    50641 gtgggccgtt gaccaatata gctatttgat taaagatgaa aatcgtttga aacgtgctcg
    50701 ccatgctgtg cttgaaaacc aacgtaccct taaagctcaa gcagcccttc aagcaggtga
    50761 tttggaaaca tttggtcgtt tgatgaatgc gtcacacgtt tctctggagc atgattataa
    50821 agtaactggt ttggaattag atacccttgt tcacacagct tgggcacaag aaggagttct
    50881 cggtgctcgt atgacagggg ctggttttgg tggctgtgcc attgccttgg ttcaaaaaga
    50941 tactgttgag gcctttaagg aagctgtagg caaacactac gaggaagtag ttggatacgc
    51001 tccaagcttc tatatcgctg aagttgcagg tggcactcgc gttcttgact agtcaaaagg
    51061 aggctctata gtgaccttag tagataaatt tgtaacacat gtcatttctg aaagttcatt
    51121 tgaggaaatg gatcgaatct acctgaccaa tcgtgttttg gcacgagtgg gagaaggtgt
    51181 tttggaagtt gagaccaatc tggataaatt gattgacctc aaggaccagc tagttgagga
    51241 aaccgttcga ttagagacga ttgaggatag tcagactgcg cgtgaaatcc ttggtactga
    51301 actgatggac ttggtgactc cttgtccgag tcaggtcaat cgtgacttct gggaagccta
    51361 tgcctactct cctgagcaag cgatagagga tttttatcaa ctcagtcaga aaaatgacta
    51421 catcaaactc aaggccattg ctaaaaatat cgcttatcgt gttccatctg attatggaga
    51481 acttgaaatt accatcaatc tctctaagcc tgaaaaggat ccgaaagaga ttgcggtagc
    51541 caagttggtg caagctagta attatcctca gtgtcaactt tgtttagaga atgagggtta
    51601 ccatggtcgg gtcaaccacc cagctcgcag caaccaccgg attatccgtt ttgaaatggt
    51661 tggtcaggaa tggggtttcc agtattcgcc ctatgcttac tttaatgagc attgtatctt
    51721 tttagatggc cagcatcgtc ccatggccat tagtcgtcag agttttgaac gtctgttggc
    51781 tatcgtagag cagtttccag gatattttgc tggatctaat gccgacctgc cgattgtggg
    51841 gggctctatt ctaactcatg atcattatca gggaggccgt cacgtatttc ctatggaatt
    51901 ggctcccttg caaaagacct tccgatttgc tggttttgag caggtcaagg ctggaattat
    51961 caagtggcca atgtctgtcc tacgcttgac ttcggattcc aaagaggatt tgattaactt
    52021 agctgataag attttccagg aatggcgtca gtattcagat tctagtgtgc agattttagc
    52081 agagacagac gagacaccgc atcacaccat cacaccaata gcgcgtaagc gtgatggaca
    52141 gtttgagttg gacttggtct tgcgagacaa tcagacttca gcagagcatc ctgatggcat
    52201 ctatcatccc cacaaggatg tccaacatat caagaaggaa aatatcggct tgattgaggt
    52261 catgggcttg gcaatcttgc caccacgtct gaaagaagaa gtggagcaag tcgctagcta
    52321 tcttgtagga gaagctgtta cagttgccga ttatcatcag gagtgggcag accaactcaa
    52381 atcccaacat ccagacctaa cggataaaga aaaagccctt gcaatcgtca aggactctgt
    52441 gggtgctatc tttgtgcgtg tacttgagga tgcaggagtc tacaagcaga cagaacaagg
    52501 tcagacagcc tttatgcgct ttgtggaaca ggtcggaatt ttactagact aggagctttc
    52561 tccttgcaca gtcctataaa aagtagtatc atagtgtcgt ttgaaatatc aggaggaaac
    52621 gatgaaagac tttcattttg acgctatatc tgcctttgaa aattacgaaa ttgaaaaaat
    52681 gagagatggt cacgttgtgg tgacgaccaa agtagtgaac tcgtcgctca actactatgg
    52741 caatgcccat ggtggctatc tattcacgct atgtgaccaa atcagtggtt tggtggttat
    52801 ctcgctggga cttgatgggg tgacactcca atcctctatc aactacctta aggcaggaaa
    52861 actcgacgat gtattgacca ttaaaggaga atgtgtccat caaggtcgta caacctgtgt
    52921 aatggatgtg gatatcacca atcaagaagg cagaaatgtc tgcaaagcaa cctttaccat
    52981 gtttgtcaca ggccagcggt cagaagacag acaggtaagg atataaagag taatttaata
    53041 gagtgaggtg aattttggaa tttataagat ttgccttact cattttttga tattgatatg
    53101 atttaattct aaaatagaaa atttagtaat gtggtagaaa atacaagagt tgtgttttaa
    53161 tttctatggt ataattaaaa gcatgaagat aaaagaaata aagaaagtta ctttacaacc
    53221 gttcacgaaa tggacaggtg gtaaaagaca attattgcct gttattagag aattaatacc
    53281 taaaacctat aacaggtatt tcgaaccttt tgttggaggt ggagctttat tttttgattt
    53341 ggctcctaaa gatgcagtta ttaatgattt taacgctgaa ctaataaatt gctatcaaca
    53401 aattaaggac aatcctcaag aattgattga aattttgaaa gttcatcagg aatataattc
    53461 aaaagaatat tatttagatt tacgttctgc agatcgtgat gaaagaatag atatgatgtc
    53521 cgaagtacaa agagctgcac gtattctata tatgttgaga gtgaacttta atggtctata
    53581 tcgtgtgaat tctaagaatc aatttaatgt tccatatgga cgttataaga atcctaaaat
    53641 tgttgatgag gaattgatat ctgctatttc agtttatata aataacaatc aactagaaat
    53701 taaagtggga gattttgaaa aggcaattgt agatgttcga acaggagatt ttgtgtattt
    53761 tgaccctcca tatattccat tgtctgagac gagtgcattt acgtcttata ctcatgaggg
    53821 attctctttt gcagatcaag taagattaag agatgccttt aagagattga gtgatacagg
    53881 agcttatgtt atgttatcaa attcttctag tgctttagta gaggagttgt ataaggattt
    53941 taatatacat tatgttgaag ctacccgaac taatggagca aaatcttcaa gtcgaggaaa
    54001 aatttctgaa attatagtca caaattatga aaaataacga atataagtat ggaggtgttc
    54061 ttatgacaaa accatactac aataaaaata agatgattct tgttcattca gatacgttca
    54121 agttcttatc aaaaatgaaa ccagaaagta tggatatgat ttttgctgat ccaccttatt
    54181 ttttaagtaa tggtggaata tctaattctg ggggacaagt agtttctgtt gataaaggag
    54241 attgggataa aatttcttca ttcgaagaaa aacatgagtt taatcgtaaa tggattcgcc
    54301 tagcaaaaga agttctgaag cctaatggga cggtatggat ttcaggtagt ttgcacaaca
    54361 tatactcagt tggaatggca ttagaacaag aaggttttaa aattctgaat aatattactt
    54421 ggcagaaaac aaaccctgcc cccaatttat cttgtcgtta ttttacccat tctactgaaa
    54481 ccattttatg ggccagaaaa aatgataaaa aagctcgtca ttactacaat tatgatttaa
    54541 tgaaagaatt gaatgatgga aaacaaatga aagatgtctg gaccggttct ttaacaaaga
    54601 aagttgaaaa atgggctggg aaacatccaa ctcaaaaacc agagtatttg ttagaacgta
    54661 ttattttagc ctctactaaa gagggtgact atattctaga cccatttgtt ggtagtggca
    54721 ctacgggtgt tgttgcgaag cggttaggta gaagatttat aggtatcgat gctgaaaaag
    54781 aatatttaaa aattgcaagg aagaggttgg aggcagaaaa tgaaacaaac tagaaatttt
    54841 gatgagtggt tgtcaacgat gaccgacaca gtagctgact ggacttatta tacagatttt
    54901 cctaaggttt ataaaaatgt atctagtata aaagttgctc taaatattat gaatagtctt
    54961 attggtagta aaaatataca agaggatttt ttagacttat atcaaaatta tccagaaatt
    55021 ttgaaagttg tgcctctact tattgctaaa cgtttgagag atacgattat tgttaaggat
    55081 ccgataaaag atttctattt tgattttagt aaacgaaact atagtattga agaatatact
    55141 atgtttttgg aaaaatcagg tatatttgat ttattgcaaa atcatttagt atcaaattta
    55201 gttgactacg ttacaggtgt tgaggttggt atggatacta atgggcgcaa aaatcggact
    55261 ggagatgcga tggaaaatat tgttcaaagc tatcttgaag ctgaagggta tattttagga
    55321 gagaatttat ttaaagagat tgaacaaaat gaaatagagg aaattttttc tgtcgattta
    55381 tctgctatta cgaatgatgg gaatactgtg aaacgttttg attttgtcat aaaaaatgaa
    55441 caagtgcttt atctgattga ggttaatttt tattcaggaa gtggttcaaa attaaatgag
    55501 acggctagat cttacaaaat gattgctgaa gaaactaaag ctattcctaa tgttgagttt
    55561 atgtggatta ctgatggaca aggttggtat aaggctaaga ataatttacg agaaacattt
    55621 gatattttac cattcttata taatattaac gatttggaac ataatatttt aaagaatttg
    55681 aaataggtcc aattcaatta gatgggaatt gggattgatt gtatttataa tcaatttgtg
    55741 ataaacaaaa aagaggctcg cacctctttt tcttatttct ttttatgatt taatacggca
    55801 ttgaggacaa tagcgagtag gctggctacg acgattccgt ttgagaagaa catttggaag
    55861 gctgtcggca tgctgacaaa gagattactg ttgttgagac cgacacctgc agcgattgaa
    55921 acagctgcga taaggaagtt gtgttcattg ttagcaaagt caactcgggc gaggatttgc
    55981 atcccttgaa tagatacaaa accaaacatc accagcatgg caccgccgag gacggagctt
    56041 ggaatgattt gggcaagggc accaaactta ggaagcagtc caaggagaac caggaaacca
    56101 gctgcgtagt agattggcag gcgttttttg atgcctgaca atttaaccaa accaacgttt
    56161 tgtgaaaatc cggtgtaagg gaaggtgtta aagattcctc cgagaagtac ggccaaacct
    56221 tctgcgcggt aaccgttgcg aaggcgcgtg ctgtcgattg gatcttttgt gatatcagac
    56281 aaggctagat aaacaccagt tgactcaacc atagacaccg ttgcgatgat acacatcatg
    56341 acaatagatg agatttcaaa ggttggcatc ccaaagtaga gtggaattgg gacatggaca
    56401 agtggagcta ccgcaacagg agagaagtcc accaagccca tagtagcagc aatggcagtt
    56461 ccaacaacca gaccaatcaa aatagagata gacttgataa atcctttggt aaagatgttg
    56521 atcaagagga taatcagaac agtaatagct gcaagcaaga gactttgacc agttggctct
    56581 ggaacgttat ttcccatatt tccaatagcg acagggatca aggttaaacc aatcgtggta
    56641 ataacagatc ctgttacgat agatgggaag agattggcta cttttgagaa aatgcctgaa
    56701 acaagaacca cgtaaatccc agatgcgata agggcaccaa acatagcgcc actaccatgg
    56761 ctttgcccaa tcataatcaa gggagcgacc gactggaatg caactccaag aacgactggg
    56821 agtccaatcc caaagtattt gttgagttgg agttggagga aggttgccac cccacacatg
    56881 aagatatctg tagaaatcag gtaggtcaac tgctcagctg aatagccaag ggctgtcgca
    56941 atcatgatgg gaaccaggat agatcctgag tacatggcta gtaagtgctg caagcccaga
    57001 acggctgctt gcgagtgttt ttcttgagtt tgcattagag atctgcctcc ttaaatacga
    57061 cctgaccatt ttcaaaacga tccaagcgag caagtgatag gacagggtag cctgcttttt
    57121 caagcaaatc acgaccatct tggaaggatt tctcaatcac gataccgata gcttggactg
    57181 tggcaccggc ctgttcgatg atttgaatca agcctttagc agcttggcca ttagcaagga
    57241 aatcgtcgat aatcaaaacc ttgtcctctg gtgagaggaa ttttccagcg atagaaacag
    57301 tgctggtcac ctgcttggta aaggagtaga cttgagcagt taagatgcct tcgttcatgg
    57361 tgatgttctt agcttttttg gcgaaaatca tgggaacgtt taaggcttca gctgtaaaaa
    57421 cggctggggc aatacccgac gcttcaatgg ttacgacctt ggtaatgcca gtagcagcaa
    57481 atttttccgc aaaaacctta ccaatctctc gcatcaagct aaagtcaact tggtgggtta
    57541 aaaaggaatc taccttgagg atgttatcac ccaagatatg cccatccttg aggatgcgct
    57601 cttctaataa tttcataaga cctcctaaag tctaaaagtt aatttacttg ttgtttaaat
    57661 atttctatag tgatcccttt tgctaatact atatatttga taaaactatt acgagcgaag
    57721 cgagtcttat caaatatttc ccgttgtagt ggtatcatag acaataatct tgttattgtc
    57781 tatgacggga tttttgagag taaaatagtt cggggaacta ttttagccta agcctagaaa
    57841 tgaaagagct aggggctcaa aaattaggga tgaaattccc tggattcctg aaattattca
    57901 caggataatt tcacctcccg tccgcactaa ttaagggaaa tattaaaaat attcaaaaaa
    57961 gacctactta atctctaagt aagtccccta aatagacatg gcaaaaacgg ccatatctca
    58021 ctgctgactt acttattgtt aggtgttccg gcaccttgta gaaacgtcgt gccaattcac
    58081 gacataaaca agtaaaacga tattcaattt taaataggct tgagccaatg tttttatttt
    58141 acactaaata actttagaaa tcaactattt tgttagtgtt ttggtttaaa aagcgaacaa
    58201 aaataagaga gggtgaacaa aaactccatt gtaagctaac atttatacta aatgaaaatc
    58261 aaagagcaaa ctaggaagct atccacaacc tcaaaacact gttttgaggt tgtggataga
    58321 attgacagag tcagtatcat atacctacgg taaggcgacg ttgacgtggc ttgaagagat
    58381 tttcgaagag tattagaaga tttttccatc ataaaaggca tactatcaag tttttagaca
    58441 cctgacaata tgcctttttc taactttaaa gacttttccc aatttttatt attctactcg
    58501 ctaaatctta aaaaatagcc atctggatcc aaaactgcaa atttatgagg atagatatag
    58561 ggatcactga cacgaaactt tcttttggtc aagagacgat aaataggata gtttgcctcc
    58621 atcacttttt gatagagttt tggaacatcc tttatgccaa aggagagatt gactccacga
    58681 ccaaaggggt aggtcagttc agctagttga tcctttgttc cctcctctaa cattagttga
    58741 cactcttcaa gagaaagaga aagttttctt ctggacgtta gtattcaatc ctaaaaccca
    58801 gtaaaccaca gtagaaggac cgggactgtt cgatattcga tacaagaaac tcgggaatga
    58861 ccgcattgta gtccatatag aaaatcctta caagtcaatt tccaagacaa tcggtgtatg
    58921 gtcttggcga gcacctgagt caatcatatc agatttagtg accttgtcag cgatacggtt
    58981 acttgtgagc cagtagtcga ttctccagcc tgtattgttg attttagaag ttttgctgcg
    59041 ttgtgcccac caagtgtagc gttcaggaac atcgccatga acatggcgga aggtgtctgt
    59101 aaatccagtt gccaaaaggt tggtaaatcc agcacgttcc tcgtcagtaa atccaggtga
    59161 acggcggttg ctagcaggat ttgcaaggtc gatttcattg tgggctacgt tgtagtcacc
    59221 ggtcgcaagg actggttttt ctttgtctag ttcagccaaa tactcagcat atttaacatc
    59281 ccagacttgg cgttcttcca agcgtttgag accgtcacca gcgtttggag tgtaaacttg
    59341 ggttacgaaa aatgcatcaa attctagagt gatgatacgg ccttccaagt ccatggtaga
    59401 aggggcaccg atttctggaa aactgatcgt aggcgtaagt tctttcttat aaaggaacat
    59461 ggttccagca tagcctttac gggcaggctc ttgggaagaa cgccacgtgt tttcgtagcc
    59521 tgggaagagt tcttctaaaa tttccacgtg tttctttgta ggtcctttgg cagaaagctt
    59581 ggtttcttgg atagcaatga tatcagcatt ttcagcgacc aaggtttgta ggacttcttg
    59641 ggacaatttg gcacgagctg agtcactagt tagggcagcg tttagggaat caatattcca
    59701 tgagataagt ttcataaagt tacctttttc attcagatta tagattttat tataccaaaa
    59761 aaagatctat ttccccaacg tatagtttga aaaattactc tctttcgttt ataattaaga
    59821 atgattttat gaaagggagt gaaaatacat gaaattctac tcttatgact atgtactcag
    59881 ccaaatcggt cagcaaaatg gtatcatggt tggctttggg attgttctat tagctgtgac
    59941 agtttttttt gcttttaagg cataccatga taaaaaggga accgaatttc gtgagttggt
    60001 catgatttca gctctggcct tatttagctc tgcttttggt cagcatcacg acttatcaaa
    60061 acaatcaagt ttctaacaat aaatttcaaa cttcacttca tttcatcgag gttgtttcca
    60121 aagatttggg agtagacaag tcagaagtct atgttaatac ttccacaaac acagatggcg
    60181 cacttatcaa ggtgggagat cgctattatc gtgccctaaa cggaagtgag ccagacaagt
    60241 acctgttaga gaaagtcgaa ttgtataaga cagacgcaat tgaactggtg gatgtgaaca
    60301 aatgacactt aattatatcg aaattttaat caaactggtc ttgactctca aatggctcaa
    60361 caacaatgtt cactttgtga aacgtttgat tgatggtaag ccaactctcc ttatcaaaaa
    60421 tgggaatatt gacccataaa cctgtcgttc agttggtttg tctgcatcgg atgtagccct
    60481 caaacttcgt agccaaggga ttttccagat gaagcaagtc aaacgagctg tgcaagagca
    60541 aaatgggcaa ctcatcgttg tgcaaatggg agatgaaaat cctaagtatc cagttgtgac
    60601 tgacggtgtg attcaagtag atgtcttgga atcgattggt cgtagcgaag agtggttgct
    60661 tgataacctc agtaaacaag ggcatgacaa tgtagccaat atctttattg ctgaatatga
    60721 caagggtact gttacagtcg taacttatga ataagaaaaa cctggggtct tgtactcttc
    60781 gaaaatctct tcaaaccgcg tcaacgtcgc cttgccgtat gtaggttact gacttcgtca
    60841 gttctatcta caacctcaaa gcagtgcttt gagcagcctg cggctagttt cctagtttgc
    60901 tctttgattt tcattgagta ttggcctcag gtttccattt gcaatcagaa agggatttta
    60961 tgtccattat tcaaaaactc tggtggtttt tcaagttaga aaaacgccgt tatctagtcg
    61021 gaattgtggc cctggtcttg gtttccgtcc tcaatctcat tcctcctatg gttatggggc
    61081 gggtcattga tgccatcaca tcggggcaat taacccagca ggacctcctt cttagcctat
    61141 tttacttgct acttgcagcc tttggtatgt actatttgcg ctatgtgtgg cgtatgtata
    61201 tccttgggac ctcttattgc ttgggacaga tcatgcggtc tcgcttgttt aagcatttca
    61261 caaaaatgtc gtcagccttt tatcaaacct atcggacggg tgatctgatg gcacacgcaa
    61321 ccaatgatat caatgccttg actcgtttag caggtggcgg tgtcatgtct gcggtggatg
    61381 cctctatcac ggctctggtg actttgttga ccatgctctt tagcatctca tggcagatga
    61441 ctcttgttgc cattctcccc ctacctttca tggcctatac gactagtcgc ctagggagaa
    61501 agactcataa ggcctttggc gaatcccaag ctgctttttc tgaactcaat aacaaggtac
    61561 aggagtccgt atcaggtatc aaagtgacca agtctttcgg ttatcaggca gacgagttga
    61621 agtcttttca ggcagtcaat gaattaacct tccaaaagaa cctgcaaacc atgaaatatg
    61681 atagtctctt tgaccctatg gttctcttgt ttgttggttc gtcctatgtt ttaacgcttt
    61741 tggttggctc cttgatggtt caggaagggc agattacagt tgggaatcta gtcaccttta
    61801 tcagctattt ggatatgctg gtctggcctc ttatggccat cggcttcctc tttaatacta
    61861 ctcagcgagg gaaggtttct taccagcgga ttgaaaatct tttgtctcag gaatctcctg
    61921 tacaagaccc tgagtttcct ctggatggta ttgaaaatgg gcgtttggag tatgccattg
    61981 acagctttgc ttttgaaaat gaggaaacac tgacggatat tcactttagt ttggcaaaag
    62041 ggcaaaccct gggcttggtt gggcagacag gctctgggaa aacgtcctta atcaagctcc
    62101 tcttgcgtga atacgatgtg gataagggtg ccatttatct aaacggtcac gatattcggg
    62161 actatcgtct gacagacctt cgcagtctca tgggctatgt tcctcaggac cagtttctct
    62221 ttgcaacttc aatcctagac aatatccgct ttggcaatcc taacttgccc ctttcagcgg
    62281 tcgaggaagc tactaagcta gcccgggttt accaagatat tgtagacatg cctcaaggat
    62341 ttgatacgct gattggtgaa aaaggagtca gtctttctgg tggtcaaaag caacggttgg
    62401 ctatgagtcg ggctatgatt ttaacccctg atatcttgat tttggatgat tccttatccg
    62461 ccgtagatgc caagacagag tatgcgatta tcgacaacct caaggagacg cgaaaggaca
    62521 agacaaccat tatcactgcc catcgcctca gtgctgttgt ccatgcagat tttattttag
    62581 ttctacaaaa tggtcaaatt atcgaacgag gcaggcacga agacttgcta gcgatggatg
    62641 gctggtatgc ccaaacctac cagtctcagc agttagaaat gaaaggagaa gaagatgcag
    62701 aataaacaag aacaatgggc tgtattgaag cgcttgatgt cttatctcaa gccttataga
    62761 ctcctgacct ttttggcact cagttttctc ctagcgacga cggtcattaa aagtgtcata
    62821 cccctcgtgg cttcccactt tatcgaccag tatctcagca atcttaacca actagccgtt
    62881 accgttttgc tggtctacta tggtctctac atcctacaaa ctgtagttca gtatgtcggc
    62941 aatcttctct ttgcgcgcgt gtcttacagt attgttagga atattcgtcg ggatgccttt
    63001 gccaatatgg aaaagttggg catgtcttat tttgacaaga cgccagcagg ctctatcgtc
    63061 tctcgtttga caaatgacac cgagactatc agtgatatgt tttcggggat tttatctagc
    63121 tttatctcag cagttttcat ctttctgaca accctttata ccatgttggt gctggatttt
    63181 cgtttgacgg ctttagtctt gctctttctt cctttgattt tccttttggt taatctctat
    63241 cgaaaaaagt cagtgaaaat catcgagaaa accagaagtc tcttgtcaga tatcaatagt
    63301 aagctggcag agaatatcga gggaatcagg attattcagg cttttaatca agagaagcgc
    63361 ctgcaggcag aatttgatga aatcaaccaa gagcacttgg cctatgccaa ccgttctgta
    63421 gccttggatg ccctcttttt gagacctgcc atgagtttgt tgaaacttct aggttatgct
    63481 gtcttgatga cctactttgg ctatcgtggt ctttctatcg ggataacggc cgggaccatg
    63541 tatgctttta tccagtacat caaccgcctc tttgacccct tgattgaggt gacgcaaaac
    63601 ttttcaacac tgcaaacgtc tatggtttct gcaggccgtg tctttgccct gatagatgag
    63661 aggacctatg agccccttca agaaaatggg caggctaaag tcaaagaagg caatatccgt
    63721 tttgaacatg tgtgtttctc gtatgacggt aaacatccga ttctggatga catttctttc
    63781 tctgttaata agggtgaaac cattgccttt gtaggtcata caggttcagg gaaatcgtct
    63841 attatcaatg tcctcatgcg cttttatgaa ttccagtcag ggagagttct cttggatgat
    63901 gtggatatca gggatttcag tcaagaagag ctgagaaaaa acatcggttt ggtcttacag
    63961 gaacccttcc tctatcatgg aactattaag tccaatatcg ccatgtacca agaaatcagt
    64021 gatgagcagg ttcaggctgc ggcagccttt gtggatgcag attcctttat tcaagaactt
    64081 cctcaggggt acgactcccc tgtttccgag cgtggttcga gcttctctac tgggcagcgc
    64141 cagcttcttg cctttgctag aacagtcgcc agccagccta aaatcctgat tttggatgaa
    64201 gcgacagcca atattgactc tgaaacagaa agcttggttc aagcttctct ggcgaagatg
    64261 agacagggcc gaacaactat tgctatcgct caccgccttt ctactattca agacgccaac
    64321 tgcatctatg tcttggataa gggacgcatt atcgagagtg gaacccatga ggaactcttg
    64381 gctctgggag gaacctatca caagatgtat agtttgcagg caggggccat ggccgatact
    64441 ctttgaaaat ctctttaaac catgtcagct ttatctgcaa tctcaaagct gtactttgat
    64501 tttcattgag tactagaagg aaatccttca aattacagat ttctttcacc gccttttcca
    64561 ttttgtggta taatgaaaaa tgttgacaaa tagtataata aaaacaaagg agaacagcat
    64621 gctgaaatgg gaagacttgc ctgtggaaat gaaatcaagc gaggttgagt cttactacca
    64681 gcttgtctct aaaaggaagg gttcgctgat tttcaagcgt tgcttggact gggttttggc
    64741 cttggtctta ctggttctaa cttctcccat ctttctcatc ttgagcattt ggatcaagtt
    64801 ggatagcaag gggccagtga tttacaagca agagcgtgtg acccagtaca accgtcggtt
    64861 caagatttgg aagttccgta ccatggtgac ggatgcggat aaaaaaggaa gtctggtgac
    64921 ttctgctaac gatagccgta ttaccaaggt tggaaatttc atccgacgtg tccgtttgga
    64981 cgaactgcct cagttggtca atgtccttaa aggtgagatg tcctttgtcg gtacacgacc
    65041 tgaagtgcca cgttatacag agcagtatag ccctgaaatg atggcaacct tgctcttgca
    65101 agcaggaatt acctctccag ccagcatcaa ctacaaggat gaggacacca tcatcagtca
    65161 aatgacggag aaaggtctgt cagttgatca ggcctatgtg gagcatgttc ttcctgaaaa
    65221 gatgcgctat aacctcgcct atctccgaga gtttagtttc tttggggaca tcaaaatcat
    65281 gtttcaaacc gtgtttgagg tactaaaata aagtagtcat aagaaaatga gtacagataa
    65341 aaggagcaaa tcaatgccaa attacaatat tccattttca ccgcctgata tcacagaagc
    65401 agaaattgct gaagtagcgg ataccctgcg ttctggttgg atcacaacag gtcctaaaac
    65461 aaaagaactg gagcgccgct tgtctcttta cacacagaca cctaagactg tttgtctcaa
    65521 ctctgcgaca gccgctctgg agttgatttt acgcgttttg gaaatgggac ctggtgatga
    65581 agtcatcgtt ccagccatga cctatacggc ttcatgtagt gtcattacgc acgtgggagc
    65641 aacccctgtc atggtggata tccaagcaga tacgtttgag atggactatg acctgcttga
    65701 gcaagctatc actgagaaaa ctaaggtgat tatcccagta gagctcgcag ggattgtttg
    65761 cgattatgac cgtttgttcc aagtcgtgga gaaaaaacgt gacttcttta ccgcttcaag
    65821 caagtggcaa aaggccttta accgtattgt cattgtctct gatagtgccc acgctttggg
    65881 atctacttat aaaggacaac cttctggttc tatcgctgac tttacttcct tctcattcca
    65941 tgccgttaag aactttacaa cggcagaagg tggaagtgcg acttggaaag ccaatccagt
    66001 gattgatgac gaagagatgt acaaggaatt ccaaatcctt tcccttcacg ggcaaactaa
    66061 ggatgctctt gccaagatgc aactggggtc atgggaatac gatatcgtta caccagccta
    66121 taagtgcaac atgaccgata tcatggcttc acttggtttg gtacaattgg accgctatcc
    66181 aagtttgttg caacgccgta aggacattgt ggaccgctat gatagtggtt ttgcaggttc
    66241 tcgcatccat cctttggcac acaagactga aactgtcgaa tcttcacgcc acctctacat
    66301 cacccgtgta gaaggagcaa gcctagaaga acgcaacctc atcatccaag aattggctaa
    66361 agcaggaatt gcaagtaatg ttcactacaa accgcttcct ctcttgacag cctataagaa
    66421 tcttggattt gatatgacga actatcctaa ggcctatgcc ttctttgaga atgaaattac
    66481 cctccctctt catactaaat taagcgatga agaagtagac tatatcattg agactttcaa
    66541 aacagtttct gaaaaagtgc taactttatc aaaaaaatga caaactacag tcaagcgaaa
    66601 gtgatcctgt ccctaaaaag tctaattgag tgtaaaaact gttgttttca attgataata
    66661 gtttacacct gtagttgagg cctctttctc ctcagagaga gaatttttat aggattttcc
    66721 tttcttgtgg gagtcccgtg gtttgaaata agatgtgagc aatttggtgt agcatttaga
    66781 atccttacta gacatcattt agaaaatcta gtgtcttgtt ctagttttca attcacccta
    66841 ttttttgaaa gacgtgagtt tccatgagtg agattgtgga aactcgcgtc tttttttgtt
    66901 ttcagaatat tgttcaaagt tttgtgcctg tctttcatgt tctagtcatt cttttgcatg
    66961 atagaattta tagcatgttg atattataat aatacgaata tcccatatgt ttagtgatgc
    67021 ttgctataca tcattagatc tcctgcgaga caatctataa aacacttgtc tacgattacc
    67081 tatatgccct attccagtat tttagaagca ctgcatctat ttttatcgag gttaaatcta
    67141 gcttttatag aaggtctatt taagaaatat attgtagtgt tttagtttca atccgccata
    67201 tgagcgatat ttaggtaaat atccctggcg aatgcttgta tgacaaggta tttgttcttt
    67261 catttataat ttacaacata tcaataaatt taaatatagt aaatgggata ttttatattc
    67321 aagctaagaa agatagcatc acttttgaat ggaaggctaa agagcaaact aggaagttgg
    67381 ccatagatag ctcaaaaccc tgctttgagg ttgtagatat agtaaaatga aataagaaaa
    67441 ggacaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaag tagaggtgta
    67501 ctattatagt ttcagtctac tatagaactg accaagtcag taacctagac ttagggcaag
    67561 gcggcactga cctagtttga agagatttcc gaagagtata aattttaata ttttcttgtg
    67621 ttattccttg acaattcaat ttggaaaata tatgataaag ataatgacag cggtgtcatt
    67681 ctatctattt taagaaaagt aataatcaat tgttaaaaat agtaaaaaaa ttggaggttc
    67741 tgatgaaata ttttgttcct aatgaggtat tcagtattcg taaattaaag gtggggactt
    67801 gctcggtact attggcaatt tcaattttgg gaagccaagg tattttatcg gatgaagttg
    67861 ttactagttc ttcaccgatg gctacaaaag agtcttctaa tgcaattact aatgatttag
    67921 ataattcacc aactgttaat cagaatcgtt ctgctgaaat gattgcctct aattcaacca
    67981 ctaatggttt agataattcg ttaagtgtta atagtatcag ctctaatggt actattcgtt
    68041 ccaattcaca attagacaac agaacagttg aatctacagt aacatctact aatgaaaata
    68101 agagttataa ggaagatgtt ataagtgaca gaattatcaa aaaagaattt gaagatactg
    68161 ctttaagtgt aaaagattat ggtgcagtag gtgatgggat tcatgatgat cgacaagcaa
    68221 ttcaagatgc aatagatgct gcagctcaag ggctaggtgg aggaaatgta tattttcctg
    68281 aaggaactta tttagtaaaa gaaattgttt ttttaaaaag tcatacacac ttagaattga
    68341 atgagaaagc tacaattcta aatggtataa atattaagaa tcacccttcc attgttttta
    68401 tgacaggttt atttacggat gatggtgcgc aagtagaatg gggcccaaca gaagatatta
    68461 gttattctgg tggtacgatt gatatgaacg gtgctttgaa tgaagaagga actaaagcaa
    68521 aaaatctacc acttataaat tcttcaggtg catttgctat tgggaattca aataacgtaa
    68581 ctataaaaaa tgtaacattc aaggatagtt atcaagggca tgctattcaa attgcaggtt
    68641 cgaaaaatgt attagttgat aattctcgtt ttcttgggca agccttaccc aaaacgatga
    68701 aggatgggca aatcataagt aaggagagca ttcagattga accattaact agaaaaggtt
    68761 ttccttatgc cttgaatgat gatgggaaaa aatctgaaaa tgtgactatt caaaattcct
    68821 attttggcaa aagtgataaa tctggggaat tagtaacagc aattggcaca cactatcaaa
    68881 cattgtcgac acagaacccc tctaatatta aaattttaaa taatcatttt gataacatga
    68941 tgtatgcagg tgtacgtttt acaggattca ctgatgtatt aatcaaagga aatcgctttg
    69001 ataagaaagt taaaggagag agtgtacatt atcgagaaag cggagcagct ttagtaaatg
    69061 cttatagcta taaaaacact aaagacctat tagatttaaa taaacaggtg gttatcgccg
    69121 aaaatatatt taatattgcc gatcctaaaa caaaagcgat acgagttgca aaagatagtg
    69181 cagaatattt aggaaaagta tcagatatta ctgtaacaaa aaatgtaatt aataataatt
    69241 ctaaggaaac agaacaacca aatattgaat tattacgagt tagtgataat ttagtagtgt
    69301 cagagaatag tatattcggt ggtaaagaag gaattgttat tgaggattca aagggtaaaa
    69361 taaccgtttt aaataaccaa ttttataatt tatccggtaa gtatatatca ttcatcaaat
    69421 ctaatgcaaa tgggaaagaa cctgttatac gtgatagcga tggtaatttc aatattgtaa
    69481 cggagaatgg gctttacaaa attgtaacaa ataatttaag tgataaaaac gaaaaagaaa
    69541 aaaacaaaga ggaaaaacaa tctaattcaa ataatgtaat tgatagtaac cagaagaacg
    69601 gagagtttaa ctcaagtaaa gataatagac aaatgaatga caagatcgac aataaacaag
    69661 ataataagac agaagaagta aactataaaa tagttggaga tggcagagaa actgaaaatc
    69721 atattaataa atctaaagaa atagtagatg taaaacaaaa attaccaaag actggttcga
    69781 acaagattat ggaactattc ttaacagtga caggaattgg tttacttttg acactaaaag
    69841 ggttgaagta ttatggtaaa gataaataaa atttgttcga tacaaggaag ttccgtagaa
    69901 aatgaggata ttgtaggttc acaaaatcag tatttttgga ttattggtgg agctacagat
    69961 ttatataatt ctaaagagga gataggttat tcagtctcag aagttgtaca tattttatca
    70021 gaaagtttgt cggttaattg taaagaatca aaaactctta aacaaatatt tgaaactgct
    70081 ttactcgagg ttaaagatga aatcggttta aactcata
//
LOCUS       DAVKJD010000004        61546 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKJD010000004 DAVKJD010000000
VERSION     DAVKJD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 61546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61546
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.004"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..532
                     /locus_tag="VW157_000246"
     CDS             <1..532
                     /locus_tag="VW157_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9551615.1"
                     /translation="SIIFEATDDIQFREILNQTLDLKGLKYIRTIRKAPVPVYQGGED
                     FSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSVGVIDLFRIKPIPEQIKTM
                     LSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQERFGQVGKMDYLLNEYGL
                     SESNIKEKVLEIYNAR"
     gene            765..2468
                     /gene="ilvD"
                     /locus_tag="VW157_000247"
     CDS             765..2468
                     /gene="ilvD"
                     /locus_tag="VW157_000247"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9551616.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(2629..2709)
                     /locus_tag="VW157_000248"
                     /pseudo
     CDS             complement(2629..2709)
                     /locus_tag="VW157_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(2767..3108)
                     /locus_tag="VW157_000249"
     CDS             complement(2767..3108)
                     /locus_tag="VW157_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9551617.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            3232..4083
                     /gene="rgg"
                     /locus_tag="VW157_000250"
     CDS             3232..4083
                     /gene="rgg"
                     /locus_tag="VW157_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9551618.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEELLFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            4274..5521
                     /locus_tag="VW157_000251"
     CDS             4274..5521
                     /locus_tag="VW157_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU9551619.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASILSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSAFILFLGKSKLSLSISSADGEKLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQVCVLLAIIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            5599..6888
                     /locus_tag="VW157_000252"
     CDS             5599..6888
                     /locus_tag="VW157_000252"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9551620.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLIFEKQGVALPIENALDVYIA
                     VLGDGANAKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTIKNNQTREEVQVSLETISKNFSEIFEKLGFYTQ"
     gene            6885..6962
                     /locus_tag="VW157_000253"
                     /pseudo
     CDS             6885..6962
                     /locus_tag="VW157_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7229..7543
                     /locus_tag="VW157_000254"
     CDS             7229..7543
                     /locus_tag="VW157_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551621.1"
                     /translation="MWVLVFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVNVDEEWVKLRSVDKKGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            7553..7759
                     /locus_tag="VW157_000255"
     CDS             7553..7759
                     /locus_tag="VW157_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9551622.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            7759..7956
                     /locus_tag="VW157_000256"
     CDS             7759..7956
                     /locus_tag="VW157_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551623.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            7959..8693
                     /locus_tag="VW157_000257"
     CDS             7959..8693
                     /locus_tag="VW157_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9551624.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            8705..9346
                     /locus_tag="VW157_000258"
     CDS             8705..9346
                     /locus_tag="VW157_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9551625.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLVLGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            9364..9594
                     /locus_tag="VW157_000259"
     CDS             9364..9594
                     /locus_tag="VW157_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551626.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            9630..9710
                     /locus_tag="VW157_000260"
                     /pseudo
     CDS             9630..9710
                     /locus_tag="VW157_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            9945..11708
                     /gene="aspS"
                     /locus_tag="VW157_000261"
     CDS             9945..11708
                     /gene="aspS"
                     /locus_tag="VW157_000261"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9551627.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            11686..12627
                     /locus_tag="VW157_000262"
     CDS             11686..12627
                     /locus_tag="VW157_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9551628.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(12631..12714)
                     /locus_tag="VW157_000263"
                     /pseudo
     CDS             complement(12631..12714)
                     /locus_tag="VW157_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
     gene            complement(12748..12828)
                     /locus_tag="VW157_000264"
                     /pseudo
     CDS             complement(12748..12828)
                     /locus_tag="VW157_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(12964..13950)
                     /locus_tag="VW157_000265"
     CDS             complement(12964..13950)
                     /locus_tag="VW157_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9551629.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(13960..14760)
                     /locus_tag="VW157_000266"
     CDS             complement(13960..14760)
                     /locus_tag="VW157_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9551630.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(15013..15855)
                     /locus_tag="VW157_000267"
     CDS             complement(15013..15855)
                     /locus_tag="VW157_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9551631.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(15857..17149)
                     /locus_tag="VW157_000268"
     CDS             complement(15857..17149)
                     /locus_tag="VW157_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9551632.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(17260..18531)
                     /locus_tag="VW157_000269"
     CDS             complement(17260..18531)
                     /locus_tag="VW157_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9551633.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            19081..20598
                     /gene="malQ"
                     /locus_tag="VW157_000270"
     CDS             19081..20598
                     /gene="malQ"
                     /locus_tag="VW157_000270"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9551634.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            20624..22882
                     /gene="glgP"
                     /locus_tag="VW157_000271"
     CDS             20624..22882
                     /gene="glgP"
                     /locus_tag="VW157_000271"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU9551635.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(23927..24454)
                     /locus_tag="VW157_000272"
     CDS             complement(23927..24454)
                     /locus_tag="VW157_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551636.1"
                     /translation="MLIVSAIPSVSAVYASEQVSQIETNMELQPVTSLTEEQINTLAN
                     EIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGITVNQMGALLDTAIALA
                     LGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALEFAMNLLSPGTYLAQL
                     WDKNDAIPNNGRINF"
     gene            complement(25120..25968)
                     /locus_tag="VW157_000273"
     CDS             complement(25120..25968)
                     /locus_tag="VW157_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9551637.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            26120..26398
                     /locus_tag="VW157_000274"
     CDS             26120..26398
                     /locus_tag="VW157_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551638.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            26400..28463
                     /locus_tag="VW157_000275"
     CDS             26400..28463
                     /locus_tag="VW157_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9551639.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(28541..29797)
                     /locus_tag="VW157_000276"
     CDS             complement(28541..29797)
                     /locus_tag="VW157_000276"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9551640.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            29939..32404
                     /locus_tag="VW157_000277"
     CDS             29939..32404
                     /locus_tag="VW157_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9551641.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNTTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSIGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            32469..33380
                     /locus_tag="VW157_000278"
     CDS             32469..33380
                     /locus_tag="VW157_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9551642.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            33610..34308
                     /gene="dapD"
                     /locus_tag="VW157_000279"
     CDS             33610..34308
                     /gene="dapD"
                     /locus_tag="VW157_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9551643.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            34376..35506
                     /locus_tag="VW157_000280"
     CDS             34376..35506
                     /locus_tag="VW157_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9551644.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            35518..36057
                     /locus_tag="VW157_000281"
     CDS             35518..36057
                     /locus_tag="VW157_000281"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9551645.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            36041..36718
                     /locus_tag="VW157_000282"
     CDS             36041..36718
                     /locus_tag="VW157_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9551646.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(36785..37684)
                     /gene="galU"
                     /locus_tag="VW157_000283"
     CDS             complement(36785..37684)
                     /gene="galU"
                     /locus_tag="VW157_000283"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9551647.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLIDDYKRTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(37706..38722)
                     /locus_tag="VW157_000284"
     CDS             complement(37706..38722)
                     /locus_tag="VW157_000284"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9551648.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            38944..39347
                     /locus_tag="VW157_000285"
                     /pseudo
     CDS             38944..39347
                     /locus_tag="VW157_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(39462..39887)
                     /locus_tag="VW157_000286"
     CDS             complement(39462..39887)
                     /locus_tag="VW157_000286"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9551649.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGETCLTE"
     gene            complement(39955..40605)
                     /gene="phoU"
                     /locus_tag="VW157_000287"
     CDS             complement(39955..40605)
                     /gene="phoU"
                     /locus_tag="VW157_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9551650.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(40620..41372)
                     /gene="pstB"
                     /locus_tag="VW157_000288"
     CDS             complement(40620..41372)
                     /gene="pstB"
                     /locus_tag="VW157_000288"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9551651.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(41374..42189)
                     /gene="pstA"
                     /locus_tag="VW157_000289"
     CDS             complement(41374..42189)
                     /gene="pstA"
                     /locus_tag="VW157_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9551652.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(42182..43045)
                     /gene="pstC"
                     /locus_tag="VW157_000290"
     CDS             complement(42182..43045)
                     /gene="pstC"
                     /locus_tag="VW157_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9551653.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(43163..44038)
                     /locus_tag="VW157_000291"
     CDS             complement(43163..44038)
                     /locus_tag="VW157_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU9551654.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(44205..45536)
                     /locus_tag="VW157_000292"
     CDS             complement(44205..45536)
                     /locus_tag="VW157_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9551655.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(45537..46244)
                     /locus_tag="VW157_000293"
     CDS             complement(45537..46244)
                     /locus_tag="VW157_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9551656.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(46328..46630)
                     /locus_tag="VW157_000294"
     CDS             complement(46328..46630)
                     /locus_tag="VW157_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9551657.1"
                     /translation="MTADEIWYFHAGSPLTVHMITADGHYEVVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            complement(46617..46838)
                     /locus_tag="VW157_000295"
     CDS             complement(46617..46838)
                     /locus_tag="VW157_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9551658.1"
                     /translation="MNKSYFNPLAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINL
                     AGKGRALYTSIYFLLRRNESFTFSPIDSR"
     gene            46800..47358
                     /locus_tag="VW157_000296"
                     /pseudo
     CDS             46800..47358
                     /locus_tag="VW157_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(47673..49364)
                     /locus_tag="VW157_000297"
     CDS             complement(47673..49364)
                     /locus_tag="VW157_000297"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9551659.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            49500..49946
                     /gene="argR"
                     /locus_tag="VW157_000298"
     CDS             49500..49946
                     /gene="argR"
                     /locus_tag="VW157_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9551660.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            49997..52531
                     /gene="mutS"
                     /locus_tag="VW157_000299"
     CDS             49997..52531
                     /gene="mutS"
                     /locus_tag="VW157_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9551661.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQTLAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            52678..54372
                     /gene="patA"
                     /locus_tag="VW157_000300"
     CDS             52678..54372
                     /gene="patA"
                     /locus_tag="VW157_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9551662.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTDV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPYEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(54702..54971)
                     /locus_tag="VW157_000301"
                     /pseudo
     CDS             complement(54702..54971)
                     /locus_tag="VW157_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            55163..56929
                     /gene="patB"
                     /locus_tag="VW157_000302"
     CDS             55163..56929
                     /gene="patB"
                     /locus_tag="VW157_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9551663.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMGLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            57068..57757
                     /locus_tag="VW157_000303"
     CDS             57068..57757
                     /locus_tag="VW157_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9551664.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            57761..57940
                     /locus_tag="VW157_000304"
     CDS             57761..57940
                     /locus_tag="VW157_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551665.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            57927..59276
                     /locus_tag="VW157_000305"
     CDS             57927..59276
                     /locus_tag="VW157_000305"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9551666.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWETFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            59400..60860
                     /locus_tag="VW157_000306"
     CDS             59400..60860
                     /locus_tag="VW157_000306"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9551667.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTDETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            60874..61308
                     /locus_tag="VW157_000307"
     CDS             60874..61308
                     /locus_tag="VW157_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9551668.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
     gene            61430..61501
                     /locus_tag="VW157_000308"
     tRNA            61430..61501
                     /locus_tag="VW157_000308"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:61463..61465,aa:Glu,seq:ttc)
BASE COUNT        18001 a        11675 c        13387 g        18483 t
ORIGIN      
        1 atcgatcatt tttgaagcaa ctgatgatat ccaatttcgt gaaatattga accagacatt
       61 agacttaaaa ggactaaaat atattcgaac aattagaaaa gctccagttc ctgtgtatca
      121 aggtggagaa gatttttcta aaggctacat tgagttaagg catggtgaag atcttgtaat
      181 cgttgcttct ggtataatgg ttgctccaag tattcgagtt gcagatgaac tgtctaaatt
      241 aggttattca gtaggtgtga tagatttatt tagaatcaaa ccgataccag aacagataaa
      301 aacaatgtta agtggaaaaa ctatatttac tgtagaaaac cacaatcaga taggtggaat
      361 tggcagtgct ttatgtgaat tattctctga tgatggaata acaaaaattc atcggatggg
      421 tgttcaagaa agatttggtc aagtagggaa aatggattat cttcttaatg agtatggttt
      481 gagtgaatcg aatataaaag agaaggttct agaaatttat aatgcaagat agatgttaaa
      541 tacacttatc tgaaagaatt tctgtaaaaa ttacattacg ttatttaaag aaagcggatt
      601 aaaaagcata ttctggtcat cagaaagtgc actaatccgc tttgttgttt ttaattttaa
      661 atatttcaca tgcgtataga attttctgaa aattcaagaa ttttattctg ttcattgcct
      721 ttaaattgtg ctataatagt aaaaactgaa atgggaggga taagatgact gaattagata
      781 aacgtcaccg cagtagcatc tatgacagca tggttaaatc acctaaccgt gctatgcttc
      841 gtgcgactgg tatgacagat aaggactttg aaacatcgat tgtgggagtg atttcgactt
      901 gggcggaaaa tacaccatgt aacattcact tgcatgattt cgggaaactg gctaaagaag
      961 gtgtcaaatc tgcaggcgct tggcctgtac agtttggaac cattaccgta gcggacggga
     1021 tcgctatggg aacgcctggt atgcgtttct ctctaacatc tcgtgacatc atcgcggact
     1081 ccatcgaggc ggctatgagt ggtcacaacg tggatgcctt cgtcgctatc ggtggctgtg
     1141 acaagaacat gcctggatct atgattgcta ttgctaatat ggatatccca gctattttcg
     1201 cctatggtgg aactattgca ccgggaaatc ttgatggtaa agatatcgac ttggtttctg
     1261 tctttgaagg tatcggaaaa tggaaccacg gtgacatgac agctgaggac gtgaaacgtc
     1321 ttgaatgtaa tgcctgccct ggccctggtg gttgtggtgg tatgtatact gctaatacca
     1381 tggcaactgc tatcgaagtt ctagggatga gtttgccagg gtcatcctct cacccagctg
     1441 aatcagctga taagaaagaa gatatcgaag cagcaggacg tgctgttgtt aagatgttgg
     1501 aacttggtct caaaccatca gatatcttga ctcgtgaagc ctttgaagat gctattactg
     1561 taacgatggc tctcggtggt tctacaaacg ccactcttca cttgctcgcc attgcccatg
     1621 ccgcaaatgt tgacttgtca cttgaggact tcaatacgat tcaagaacgt gtgcctcact
     1681 tggccgactt gaaaccatct ggtcagtatg tcttccaaga cctctacgaa gtcggtggtg
     1741 tccctgcggt tatgaagtac ttgttggcaa atggtttcct tcacggagat cgcatcacat
     1801 gtactggtaa gactgtagct gaaaacttgg ctgactttgc agacttgact ccaggccaaa
     1861 aagttatcat gccacttgaa aatccaaaac gtgcggatgg tccgcttatc atcttgaacg
     1921 ggaaccttgc tcctgacggt gcagttgcca aggtatcagg tgttaaagtg cgtcgtcacg
     1981 ttgggccagc taaggtcttt gactcagaag aagatgcgat tcaggccgtt ctgacagatg
     2041 aaatcgttga tggcgatgta gtcgttgttc gttttgttgg acctaaaggt ggtcctggta
     2101 tgcctgagat gctgtcactt tcttcaatga ttgttggtaa aggtcaggga gataaggtgg
     2161 ccctcttgac ggacggacgt ttctctggtg gtacttatgg tctggttgtt ggacatatcg
     2221 ctcctgaagc tcaggatggt ggaccaattg cctatctccg taccggcgat atcgttacgg
     2281 ttgatcaaga taccaaagaa atttccatgg ccgtatccga agaagaactt gaaaaacgca
     2341 aggcagaaac aaccttgcca ccactttaca gccgtggtgt cctcggtaaa tatgcccaca
     2401 tcgtatcatc tgcttcacgc ggagccgtga cagacttctg gaatatggac aagtcaggta
     2461 aaaaataaac tcatactcga aaatctcttc aaatcacgtc aacgtcgcct tgccgtaggt
     2521 atggttactg acttcgtcag ttctatccac aacctcaaaa cagtattttg agctgacttc
     2581 gtcagttcta tccataactt caaaacagta ttttgagctg acttcgtcag ttctatccat
     2641 aacttcaaaa cagtgttttg agcaacctgc ggctagtttt ctagtttgcc ctttgatttt
     2701 cattgagtat caaaaagcaa gccatttttg gcttgctttt ttcatcttta aaagtgattg
     2761 gcctgcttaa cgaggtggta gtccaagggc aatacggcca tagcgactga ttcgtgtgat
     2821 tttccaagca ggcgaccagg taacttcaac cttgatatct ttgataccct cgatttgttt
     2881 cagacctgcc acgatttcaa taggcaggct ttcggcgcaa tcacaggcag tatcggtgaa
     2941 ggtcatgaca atcttgcaga gccccgtttc atccagattg atttcataaa tcagacctag
     3001 attgtaaaca tccaattcca catctgtatc aaaaactttc tctagttttt cgataatttg
     3061 gtcttgcaag gccaaagcac ggtcattgat tttgatatcg tctctcatag cagtccctcc
     3121 acgtgataaa tatcctctat tttctcataa ttctgacaat ttggcaagtt tttgatgaat
     3181 tttctgaaaa atatgataaa atgaagaaaa tacggaatca aggggaagtc tatggagcag
     3241 attggaaaag tctttagaca attacgagag tcaagaaata tctcactgag acaagcaacc
     3301 ggaggacaat tttcgccgtc tatgttgtcc cgttttgaaa caggtcagag tgagctttcg
     3361 gtggaaaagt ttctatttgc tctggaaaat atatctgcca gtgtggagga gctcctcttt
     3421 ctggcgagag gtttccagta tgatacagat tctgagttga gaaaagaaat tacagatgtc
     3481 ttggaaccaa agaatatagc acctcttgag gacttatatc gcagggagta tcaaaagcat
     3541 gcccattctc acaacaaaca gaaacatatt ctaaatgcca ttattatcaa gtcttatatg
     3601 aagagcatag atgaaagggt agacttaaca gcagaggaag gaaaagtcct tcatgattat
     3661 ttgttttcta ccgagatctg gggaatctat gaactcaatt tattttcagt cagttctcca
     3721 tttttatctg tttctctttt tactagatat gtacgagaaa tggtacgtaa atctgatttt
     3781 ctaatggaaa tgtctggcaa tcgaaacctt tttcatacta tactattgaa tggcttttta
     3841 gctagtattg agtgtgaaga atttaccaat gcctattatt ttaaacgtgt tatcgaagag
     3901 catttctaca aggaaaatga gacctatttc cgaattgttt atctgtgggc agaaggtctc
     3961 cttgatagca agcaaggtag agtcaaggaa ggtcagaaaa agatggagga tgctgtccgt
     4021 atttttgaga tgcttggctg taataaatct gccgaatact atagaaatac gaccgaatgt
     4081 tgagtatctg caaaccaaga aaaaatttga agcgatagaa ctgttgccct ctctcaggtc
     4141 attacaaaag ttctatcgtt tttttgctca ttttgtttgt tgcatatatt caaaagaatc
     4201 gtttcgcaaa ttcataagtg ctatactaaa gctattcttg atgtagaact tagaacaagg
     4261 agggagatta tctatgaaac tattgtttag aaatccagct tatagaattt tgactttatc
     4321 acgcttcttc aatgcttttg gtgcttcgat tttcaacctg gtctttatcg tctatgcatc
     4381 gatcttgtca caagcatctt ttgctgttgc tatggcgaat attgtcatga ttcttccgac
     4441 cttctttaca gtttttgcag ggattcgggc agattacacg agggacaagg tcaaatggat
     4501 ggtctatagc ggtttgtttc aggcggttct attttttcta gcagccctag ttgttcagca
     4561 agctagtctc tttgcctttt ctagcctgtg tttgatcaat gtcattagtg atatcatcag
     4621 tgattttgca ggtggcctgc gtatgcctct tattaaggaa aaagtagctg aagatgatct
     4681 gatggaggct tattcttttt cacagttcat cacctatatt tcagctattg gtggtcaagc
     4741 tttcggagtc tggctcttag ctctatcggt caacaatttt tccctcgttg cgggaattaa
     4801 tgcttgcttt ttcctcgtat cagcctttat tcttttttta ggaaaaagca aattgagcct
     4861 gtcaatttca tctgctgatg gtgaaaaact aaaaaatgag aagctttcta tcaaagacca
     4921 gttcctaaca atttaccgaa atttacgtct cgtttttctt aaaagtggac agaaaaactt
     4981 tggttttatg ctctttgctg tcttgcttat taattccttg ggtggcgctt tgggtggaat
     5041 ttataatatc ttctttttga gccattctct cttgaatttt tcttatacag aggcactatt
     5101 tatcaatcaa gtctgtgttt tgttagcaat catcattagt agccttacgg gcaatgatta
     5161 ttttgggaag cagtccttgc ctagattgat gatgtgggag accgtaggac ttagtctagt
     5221 tggtttggct aatttattca atcaagtcgt gcttggtttg ctatttctct tctttactct
     5281 gtatgtgtct ggtaaagttc aaccaaagat tagtgccatg ctcatgaaaa atctagctcc
     5341 agaggttcta gctcgtacta gtaatttttt aggtctattg tttaccttat ccatacctgt
     5401 gggaacagct tgtttttcac tagtagccgt atggaacatg cagttgactt ggatgctatt
     5461 tgttggtctt tccttgctag ctattttttt aacaattctt aatctcaaaa atgatatcta
     5521 aatcctaatt tttttctcac tgttttaatc cctctattta tggtaaaata agactattaa
     5581 gtttaaagag gattccctat gaaattacaa aaaccaaaag gaacgcagga tattttacct
     5641 gctgaatctg ccaagtggca gtacgttgag ggctttgccc gtgaaatttt caaacgctac
     5701 aactacgcag aagtgcgtac gcctattttc gagcattacg aggttatcag ccgctctgtc
     5761 ggagatacaa cagatattgt aaccaaggaa atgtacgatt tctatgacaa gggtgaccgc
     5821 catattaccc tccgtccaga aggaactgcg cccgttgtcc gttcctatgt ggaaaataaa
     5881 ctcttcgccc cagaagtgca aaagccaagc aagttctact acatgggacc tatgttccgt
     5941 tatgagcgtc cacaggcagg gcgcttgcgc caattccacc agattggtgt tgagtgtttt
     6001 ggctctagca atccagctac cgatgtggaa acaatcgcta tggcagccca ttttttgaag
     6061 gaaatcggta ttcaaggtgt caaattgcac ctcaacactc ttggaaatcc tgagagccgt
     6121 gcagcctacc gccaagcctt gattgactat ttgacaccgc tcaaggagac cttgtctaag
     6181 gatagccaac gtcgcttgga ggaaaatcct cttcgtgtct tggactctaa ggaaaaagaa
     6241 gacaaggtgg cagtagagaa tgcgccgtct atcttggact ttcttgatga agaaagccaa
     6301 gctcattttg atgctgtacg tcagatgttg gaaaatcttg gagtagatta catcatcgat
     6361 accaatatgg tgcgtggtct ggactactac aaccacacca ttttcgagtt tatcacagag
     6421 attgagggca atgacttgac agtctgtgcg ggtggtcgct acgatggttt ggttgcttac
     6481 tttggaggcc ctgaaactgc tggatttggt tttgggcttg gtgtagagcg cctgcttctc
     6541 atctttgaaa aacaaggcgt ggccctccct atcgaaaatg ccctagatgt ctatatcgca
     6601 gtcttgggtg atggagcaaa tgccaaagcc ctagaactag tccaagctct tcgccaacaa
     6661 ggtttcaaag cagagcgtga ttacctcaac cgtaaactca aagctcaatt caagtcagcc
     6721 gatgtctttg cggctaagac cctcatcact ctaggagaga gtgaagtcga aagcggacaa
     6781 gtgacgatca agaacaatca aactcgagaa gaagtgcaag tgtcacttga gacaatcagc
     6841 aaaaatttct cagaaatctt tgaaaaacta ggattttata ctcaatgaaa atcaaagagc
     6901 aaactaggaa gctagccgca ggttgctcaa aacagtgttt tgaagttatg gatagaactg
     6961 atgaagtcag ctcaaaacac tgttttgagg ttgtggatag aactgacgaa gtcagtaacc
     7021 atacctacgg aaaggtgaag ctgacgtggt ttgaagagat tttagaagag tattaatgat
     7081 agataaagtg gtcaggaact tactcctgac ctttttcttt tgcaaaatga caaaaatcat
     7141 aaactttttg ataactatac ttgtcaaaaa gaaaaagata tgttacaata aattcaagtt
     7201 aagaaatagg aagcagaaag gagttataat gtgggtacta gtatttatac tatttatgat
     7261 tttcttttat tctaataatt ctaaaaaaat caagaaacta gagaataaaa tcaaaaaact
     7321 tgagcgaaaa gagaaaggaa acgcagaaat gtcgagatta ttacaagaaa tgattggaaa
     7381 ggaaccaatt ataacgggag tgtatattgg gccagataac tgggaagttg tgaatgttga
     7441 cgaggaatgg gtcaaactac gtagtgttga taaaaaggga aaagaaaaat tcaagttgca
     7501 acgtattgaa gatatccaaa ccgttgaatt tgacggagag taggtgtgta acgtgcaact
     7561 aaaaaatcgt ctaaaagagc ttcgagctcg cgatggtctc aatcaaaccg acctagccaa
     7621 actggcaggg gtttccagac agaccattag cctactagaa cgggatgagt acaccccatc
     7681 cattatcatt gccctgaaaa tatctcaaat tttcaacgaa acagtcgaat cggtatttcg
     7741 tttggaggag gacgagtgat gaacaagtat aaagtgattt attatgtagt ggtcatagct
     7801 ttattagtca gtgtatttct actgattggg atggacctaa gctggtttaa tccctatcaa
     7861 agcgaccaat ttgtttgggt ctactttgct ctcatcccag taattgaatg gattgaaaag
     7921 aaatccaaaa atctagcaag tgaaaaagga gaatgaatat gaaaaagaaa cgtagattgt
     7981 tatttttgat gtctattgtc ttgggggggt tcttgggcat gtttgtaggg atgtttaagg
     8041 cacgtgtcga atcccacgaa attattttag atgtaaaagc cttgatgcca tggatatcag
     8101 ctatttgttt actgataggt ttcattagta tgtttttgac tttcaatttc ttaaagaaaa
     8161 gtagaaaatt tcattccttg tatcaagagg aaatggatga cgatctgaat gaaacctatt
     8221 atgtgcaaat gtatcggaat cttgagtttg gaaccattgc ttttaatatt acaggtgtag
     8281 cgattccatt ggctattttt atttcattaa gtgaggtgat tatattgcat acaaaccctc
     8341 aaacattttt cctttctttc ttactctttg tggtattctt agtcgctcaa aaatctcttt
     8401 ttaaaaccat tgcgattgtt cgtcagtttg atttggaatt tttcgctaca ccaaaggatg
     8461 tcttgaacta tataaattct tatgatgaag gggagcgtca ggctaatttg gaacagagtt
     8521 ttcgaatttt attccaatta caccaatatg tcttaccagc cttatatatt tttcttatta
     8581 tcatttcttt cttgacagga gagattcagt tactagcttt cttgcttgta ggagccatcc
     8641 atgtttatat caatgtgatg cagttaccta tggtaaaacg ttatttcaaa taaaggagtt
     8701 atctatgaaa ctacttaaaa accttggctg gtttcttcta gcccttctat cctttttctt
     8761 tatctatggc tttattcagg ggctcgcgac tgcgtcgctt gttttaggcg cttcacccta
     8821 tgctgttact ctactctatg tggtcttggc tggagtatat gtgtacggca tttacaaatg
     8881 gtatcagaaa gctcctgttt atattgagaa gagcggcttt aaccgattta tttggcttcc
     8941 tgccttggtt tggttcttat ctctggtcgt tcaattcttc ttgccagatg atccttcagt
     9001 aaatcagcaa atagcgacag acttgacctt gtctcaacca cttttctcat tctttgctgt
     9061 ggttattttt gctcctttga cggaagagat tgtttttaga gggatgttag cacgctatct
     9121 ctttcctaag caggacaata gtaaacgaac cctgattttt cttctggtat ctagtcttct
     9181 atttgccttg attcattttc caggtgatgt gcaacaattt tttgtctatt ttagccttgg
     9241 ttttagtttg ggtttggctt acattagcag aaaaggtctg gtctacagta tttctcttca
     9301 cgctttgaat aatttagtcg gctttttgat gattctcatg ctataataga gtcaggaggt
     9361 cacatgaaac gagtaatttt attggcagtg attcaggcag tcgttctatt tttcatcatt
     9421 ggagcgctag cttatgcctt caaaggcgat ttcttctata actatctagc agttgtcttt
     9481 gctcctattg caggtgtact gcgttttggg acggcttaca taacggaaat tgtcttgcct
     9541 cgaaaggcag ccgaaatcgc tgaaaagcgt aaagcaggca aaaattcaaa ataaaagagt
     9601 ccggtgaagt tcatcggatt tttgtattat actcaatgaa aatcaaagag caaactagga
     9661 agctaaccgc aggttgctca aaacactgtt ttgaggttgt agatgaaact gacgaagtca
     9721 gctcaaaaca tggttttgag gttgtagatg aaactgacga agtcagtaac cgtactacgg
     9781 taaggcgacg ctgacgtggt ttgaagagat tttcgaagag cattagcgct aatttttagg
     9841 gctttcactt gacttttaaa gactggcaaa cttttctgat gattttcatg aagagcctgc
     9901 gcttttatgg taaaatagta acagaataaa agaggagaga aataatgaaa cgtagtatgt
     9961 atgctggtcg tgttcgtgag gaacacatcg gacaagaaat aaccttgaaa ggatgggttg
    10021 gccgtcgtcg tgaccttggt ggtttgatct ttatcgatct tcgtgaccgt gaaggaatca
    10081 tgcagttggt tatcaaccct gaaaaagtat ctgcagaggt tatggcaaca gctgaaagcc
    10141 ttcgtagcga atttgttatt gaggtgactg gtcaggtcgc tgcgcgtgag caagccaatg
    10201 ataagttgcc aactggtgca gttgagttaa acgtgacagc tcttattgtg cttaacacag
    10261 ctaagacaac accatttgag attaaggatg gcattgaggc aaatgacgat acacgtttgc
    10321 gttaccgtta ccttgacctt cgtcgtccag aaatgttgga aaatcttaaa cttcgtgcca
    10381 aggtgacaca ctctatccgc aactacttgg atgagttgga gtttatcgac gtggagacac
    10441 cattcctttc taagtcaacg cctgaaggcg cgcgtgatta tttagtgccg tctcgtgtta
    10501 ataaggggca tttttacgct cttcctcaaa gtccacaaat cacgaaacag ctcttgatga
    10561 atgctggttt tgaccgttac taccaaatcg ttaaatgttt ccgtgacgaa gacttgcgtg
    10621 gagaccgcca gcctgaattt actcaggtcg acttggaaac gtccttcctt actgagcaag
    10681 aaatccaaga tattacagaa ggcttgatcg cgcgcgtgat gaaggaaaca aaaggcatcg
    10741 aagtaacgct accattccct cgtatgaaat acgatgatgc tatggctctt tacggttctg
    10801 acaagccaga tactcgtttt gacatgttgc ttcaggactt gacagaagtg gtcaaaggtg
    10861 tagactttaa agtcttttca gaagcacctg ctgtaaaagc gattgtggtc aaaggagctg
    10921 cggacaacta ttcacgtaaa gacatcgaca agatgacgga agtagccaaa cagtatggtg
    10981 ccaaaggtct tgcttgggtc aaggtggttg atggagaatt aaacggacca gttgccaagt
    11041 tcttgactgg tatccaagaa gaattgacaa cagcgcttgc tcttgaagat aaggacttgg
    11101 ttctctttgt ggcggatacg cttgaagtgg ctaatgcaac actgggtgcc cttcgtggac
    11161 gtattgctaa agagcttggc ttgattgata atgataagtt caacttcctt tgggtggttg
    11221 actggccgat gtttgaatgg tctgaagaag aaggccgcta catgagcgcc caccatcctt
    11281 tcacccttcc acaggaagag actgctcacg aattagaagg tgatttggct aaggttcgtg
    11341 ccattgctta cgatatcgtc ttgaacggtt atgagcttgg tggtggtagc cttcgtatca
    11401 accaaaaaga ccttcaagaa cgcatgttca aggctcttgg tttctcaact gaagaagcca
    11461 atgaccagtt tggtttcctt cttgaagcca tggactatgg tttcccacca cacggtggtt
    11521 tggctatcgg gcttgaccgt tttgtcatgt tgcttgctgg agaagaaaat atccgtgaag
    11581 tcattgcctt tcctaagaac aacaaggcaa ctgacccaat gacacaagct ccatcaacag
    11641 tcgctctcaa acaactagag gaactcagct tacaagtaga agaagatgaa acaaacaaaa
    11701 cgaattaagc ggtggcgcta ttatctgcgc cgctttgctt atcagataaa aattttacgt
    11761 gtcttacaaa gtatctctcg agaaaagtat gatgagaaga tttcggcctc tctggtctat
    11821 ggttttttat cagcagtagc agttaatttc tttttccaac cagggcatgt gtattcgagt
    11881 ggtgcaacag gtctggcaca gattatctct gccttgagta atcactggtt tggttttcat
    11941 attccgattt cgctaagctt ctacgccatt aacttccctt tgatggtctt agcttggtat
    12001 cagattggcc ataagttcac cgtctttacc tttatcacgg tatctatgag ttccttcttt
    12061 atccagtttg tccctgtggc aaccttgacg gaggatccca ttatcaattc cctttttggg
    12121 ggtgttgtta tgggtttggg gattggtttt gctcttcgaa acaatatctc cagtggtggg
    12181 acggatatcg tcagcctgac tattcgcaag aaaacgggta agaatgtcgg tagtatttct
    12241 ttcttggtaa atggaactat catgctgata gcaggtttga cctttggttg gaaatacgct
    12301 ctttattcta tgattaccat ctttgtctct agccgtgtga cagacgcagt ctttactaag
    12361 caaaagcgta tgcaggccat gattgtgaca aatcatccag agaaggtaat tgaaaaaatc
    12421 cataaaaaat tgcaccgcgg agcaaccatg atccacgatg cagaaggaac ctataatcac
    12481 gagagaaagg cagttttaat cactgtcatt acacgtgcag agtttaatga atttaaacag
    12541 attatgacac aagtggatcc aagctccttt gtctctgtct cggaaaatgt tcatattcta
    12601 ggaagatttg ttgagataga taattagtga ttaatactct tcgaaaatct cttcaaacca
    12661 cgtcagcttc accttgccgt aggtatggtt actgacttcg tcagtttcat ctacaacctc
    12721 aaaaccatgt tttgagctga cttcgtcagt ttcatctaca acctcaaaac agtgttttga
    12781 gcaacctgcg gctagcttcc tagtttgcac tttgattttc attgagtata aaaagatctt
    12841 gggaaaagac tcaatttcag tagaaaagga agtaaatctt cccacaataa aacgcatagt
    12901 atcaagtttt ttcaatacct gatagtatgc gtttttgctt ttaacgactt tttgcccagt
    12961 ctcttatttt tcgataattt tgtgggcgat caattgacgg taacaaattt gtttattgtt
    13021 tttattatca ttaataatct ggagaatagt ttcaagggaa acacgaccaa gctctaaact
    13081 attgatatcg acataggctg ccaagttgag cttgggatta accgagtcaa agctgagaac
    13141 agggacatcc agctggtgtt tggcaatata gttacaaaca ccttcagcta ggaggctatc
    13201 ggttgtgatg atagcatcaa tttgtggatc gtgcttgaat aatcgcttgc taaatttata
    13261 gcccttttct tccagaaact cgtcggcaaa gtagatgcga ttgttgtcag tggtaagttt
    13321 gtaatgttta agcgcctgtt catagcctgt taaacggtct ttggtcacga agagcttttt
    13381 acttcctccg ataaaggcaa tgcgtttgca gccttttttg atgaaatatt cagtcgcatc
    13441 aaaaccagct tgaacattgt cgttgtcgac aagtgggatg aaaggagata gagatttacc
    13501 taagataagg aaggggaact gttcttctgc gacgagtttt acgagagggt cttcttcttg
    13561 ggcatagaga aagattagcc catctacacg cttgccgtag accatttgtg aaatagcgtt
    13621 gagacgctcc ttctcatctt tccctgttgc tatctgaatg gcatagtggt tttcagatgc
    13681 gacttgagag atgccacgta gaaccgatgg aaagaaagga ttctggtaga aggcgtctga
    13741 gtcatcagga agaactaatc cgataacctg agtatagctg cttaccaagc tacgagcgtt
    13801 gaggtttggg tggtagttga gttccttcat agctttgcga acgcgttttt ttgtttcgtc
    13861 gctaatggtt gatttatttt gaataacacg ggttacggtt gaaggcgaaa caccagcagc
    13921 cttggccacg tctttaatcg taacgggcat aaaaatctcc tatttatggt aatttggatc
    13981 acggaaatgt gttttataaa agatagtgac cagatacaga acaaaaagaa tattttgtac
    14041 agtaatcagg gttgtcatat tttggccaaa taatcccaaa ataagtgtaa tcagagtcgg
    14101 caatcctaaa cagttcaaga taaagtgata gcactcttta aaggttctaa atgaaaagag
    14161 gcgtgatttc ttagtgatat aaaggagaag actagctcct agagagacga taaagaaatt
    14221 caaaccaaag aggaagctcg caccgagaac taggaagagg ctgatataga cacgattttg
    14281 ttggtaccag tctttagaaa ttgcttgggt caagctgtct ttgcttttga aactctcagt
    14341 ctgaatggct cggtaagaga tgcgggtcag ttccttgctt tccttgctga tgactagctc
    14401 atttgtatcg aaatgcagtt gcaagtcctt aggtaattcc ttgatttgac ttggaccaat
    14461 cacaacagaa ggggcttgac tagctgttcc agtataagtt aatgtgccat cgacaattgt
    14521 agcatgttca gagagatcct ggacaacctc atttgtcaga ggttcataga cattatcgat
    14581 aaaagtttct agcggatagg tctcctggga gctgttttgg atagcaatgg gtaccataga
    14641 caagctgata aggaagatac tggtaaagag aagttgaaac cagttgagct cgaaacgttt
    14701 ggacaggggc ttacgaaacc cccaaatact tgaaaaatag gaaaatggat atggaagcat
    14761 aggtatcttt ctaaaaggtg ttttctatat gagtattatt atatagagaa atgcgcttta
    14821 tttcaagtct aggagaaaaa ttgcttgcta caaggatatt atttacagtg acaattgcta
    14881 ataatcaatg aaaatcaaag tgcaaactag ccacaggttg ctcaaagcac aactttgagg
    14941 ttggagataa agctgacgtg gtttgaagag attttcgaag agtataaaat gaaaaagggt
    15001 ggcggggata aattatccct tgtcgccacc acttgtaagt cctgaaacaa agttcttttg
    15061 taggaagaag aagagaatac agattggaag ggcgatgagg atagcacctg ctgagaagta
    15121 ggcaatcttc aagtttttcg cattgttaac gaaggtttgg agacctacgg caacagtaaa
    15181 gtattctttc tcacgaagca agaaactaga gaggatgtag tccccgaaag gtcccatgaa
    15241 ggcccagaga gcttgtacgg caaccattgg gcgaacaagt ggtagaacaa tttgccagaa
    15301 gcggcggaag tgtcctgcac cgtctagttt tgcagattcg tctaaagaca ttggcactgt
    15361 atcgaagtag cctttcatga gccaagcatt catcgggata ccaccaccaa cgtagaggaa
    15421 gatgaggaac cagttgtggt taagggcgtt caacataagc gccataacga agaaggctgt
    15481 caaagcggcc atagttggca ccatttggat gatcaagaag aagaccaaac tttgtttacg
    15541 agccaagaag ttgtaacggc tgtaagcata accagcaagt acgatgatac ttgtttgaac
    15601 agccatggta attaaggcga taatcaaagt gttgaggtac caagtaccgt acaaggtttc
    15661 agtgaagagg cctttaaagt tatcaaaatt gaggtcgata ttagtatcta gtttaaaggc
    15721 tgagacgtta cctgctttaa aggctgacat aatggtaatc aacagtggat agataattac
    15781 aattgataga ccaatcaggt aaaggtaagt aaggctttga gtcagtctac gtttgagttt
    15841 aattgagtta ttcatcttag acgtcctcca tatcaaatgc gtgtagtttc ttgaatgcga
    15901 tcatagagat tgagatgaca atgatagaga taatcaaggt aacagctgcc gccattgagt
    15961 attgaggaga tgtacctgtt gtcaaacggt agatccatga gatcaagata tcggttgaac
    16021 cagctccacc tccgacacta ccaggtcctc caccattgaa gaggtacatg atagagaagt
    16081 tgttaaagtt gaaggtgtat tggctaatca aagtaggtgc cgcaacagcc aaaatcattg
    16141 ggaaagtgat gttgcggaat ttttgccaag cgttggcacc gtcaatataa gctgcttcgt
    16201 aaaggtcgtt aggaatagat tgcaagatac ccaaagtcaa aacgtagatg tatgggaatc
    16261 cgagccaacc ttgcatcata atcaaggcaa tcttagtcca agttgggtct gttttccaag
    16321 gaataagagc tccatcaagg aaaggaagga atttagccaa gattggcaat acttgagtgt
    16381 tgatagcacc gacactatcg ttaaacatgt ttgagaatgt caagatagtg atgaaggctg
    16441 ggacagccca aggaagaagg aaaataacac caaagatacg ttttcctttg ataaatggtt
    16501 ggttggcaat gatagctgtg aagataccaa ttacgatttg taaagtagaa gctgccaaag
    16561 cccaaatgat agtccaagaa agaacagaac caaaggcaga acggaaggta ctcaagctcc
    16621 aaatgtttgt aaagttggtc aaaccaaccc agtccaacaa cttgtttggt ggcaagtgtt
    16681 ggaagtcgta gttggtaaag gcgatcatca aggttacgat aactgggaag ataatcgcga
    16741 aggtcatggc aacataagat ggaatgatca agaggtaagg gaagccattt tcataaatcc
    16801 ctttgatcat gtctttgagt gtgcgtggaa ctggaattcc attgttaatg cgttttgcaa
    16861 tcgtatgtgc atctttgata tttgagaaat aaaagagtac ataaacgatt acaaagatta
    16921 gatggaaggc accacgaatc agcataaaga gggaattatc acgacctggt ttgtcaccaa
    16981 gagtgatgag gttgctcaat tcaggggttg caagtgctag gaagtaaagg acaaatacga
    17041 tggttacacc aaggaagata aaacctttgg ctttttgttt attgtaaatc tgtcctaacc
    17101 caggaatgat agacagcagg gctgctttac taggttgttg cttttccata ttaactcctt
    17161 tcataggata ctactttttt ataagaaagc taaattattt gtgtctctat tgataaattc
    17221 aaagggggat ttgatttcca ccccccttga acaaattttt tattcaccaa atttttgttt
    17281 gattgtttct ttgatcaatg ttacagcatc gttagcagct gttttagcat ctttttgacc
    17341 acttacagca tcaaagagca tatttttcgc tggatcccaa actgcagaca tttgagagat
    17401 gtttggcagt ggttgagtgt tcttgaactg tttgataaca gctgttgtca actcatcgtt
    17461 tttaccttca gcgtatgaac gagcctcagt attagctggg atttcgttag tcttatcata
    17521 taatactttt tgttgttcag ttgcaacaag gaagtctaca aatttttgag aagcttcaag
    17581 gttcttaacg gcttgaggaa tgacccaagc tttaccacca ccgaatgcag catattcttt
    17641 tccatttgga agagttggga tagttgcaac tccgtagttt actttagcat ctttaaaggc
    17701 ttgagctttc caaggtccgt cgatgatagc agctgtttta ccttcttgga attgagtttg
    17761 gattaagttt ccagcacctt ctgtatcttg cataccttta ggccattttt cgtaccaaga
    17821 tttagcgtag ttgatacctg cgatagaacc gtcgtttgca agaccgatgt ctttagcgtc
    17881 tttaccgttt tggccaaaga cgtaagcacc gttaccggca agaagtccat atgtatagta
    17941 gaagtttgtc cagtcagcta ggaaggcagt agttttacca tcttcaccag cgaatgcgta
    18001 tttgctatct ttagcaaggt tttccaagtc agcaaatgtt tttggagcat ctttcaccaa
    18061 gtctttgttg tagtacataa caagtgactc gataacggca ggagcaccgt aaactttacc
    18121 attagcagct gttacaagag atttagttgt gtcgtctgtt ttagcaccat cgctcaattt
    18181 cacttctgaa agttgtccgt cagaaccaag gctacctaca cggtcgtatg gagccatcat
    18241 aacatcaggg acattaccag attggttgtc aagagaaagt ttatcaagac ctcctagagc
    18301 atcaccagtt ttaagagtga cttttactcc agcttctttt tcataagctt tagcaacctc
    18361 ttcaatatag ctcttatatc cctcgtctac atatacagtg agttctttga cttcagatga
    18421 accagaatca gcaggcttat cagcagtttt gcttccgcaa gctaccaaaa gcaagctagc
    18481 aagtgtaaca gttccaagca ccgcagtgct cttcataaat ttagatgaca tagtgtattc
    18541 ctcccaaaga atagcaagtt ttattgataa ggaaacgcaa acgttttcct ttatgagctt
    18601 agtatagcac aaatagaaaa cggttgcaag tattttttgt aaaaatttta aaaaattttt
    18661 aagcttgatt tgatagcata attttacatt ttaatagaag aaaagtatga aatagataag
    18721 aatacagtat ttttaataaa aatatatgga atctgtctaa agagggaaat caggaggggc
    18781 tacctccctg tttctaatcc ctatctattt tctatagacg tttgtgcttt gttgcatata
    18841 aattcaagct ccctaaggag gttttgtact ttgtagactg tctcgtgaga gtcaaacttt
    18901 aggattagat agttttaagt tatctttgct aaaatgagtt agtctcctta aaaataatca
    18961 aaaaagtttt ttaaaaacgc ttgcaattat gcttgaaaag gagtatactt ataagtaacg
    19021 caaacgtttg cgtctgtaaa atacgcaacg ttccattatt ttaacacacg aggtgctatt
    19081 atgaaaaaac gtcaaagtgg tgtgttgatg cacatctctt ctcttccagg agcttacgga
    19141 atcggatcat ttggtcaaag tgcttacgac ttcgttgatt tcttggtccg tacaaaacaa
    19201 cgttactggc aaatccttcc attaggagca actagttacg gggattctcc ttaccaatct
    19261 ttctcagcct tcgcaggaaa cactcatttt atcgatttag atatcttggt ggagcaaggt
    19321 ttgttggaag caagtgacct tgaaggagtt gactttggta gcgatgcgtc tgaagttgac
    19381 tatgctaaaa tctactatgc acgtcgtcct cttttagaaa aagcggtgaa gcgtttcttt
    19441 gaagtcggag atgttaaaga ttttgagaaa tttgctcaag acaaccaatc atggcttgag
    19501 ctctttgctg agtatatggc tatcaaagag cattttgaca atcttgcttg gactgaatgg
    19561 ccagatgcag atgctcgtgc tcgtaaagct tcagcacttg aaagctatcg tgagcaattg
    19621 gcagacaagt tggtttacca ccgtgtgact caatacttct tcttccaaca atggttgaaa
    19681 ttgaaagctt acgctaacga caaccacatc gaaatcgttg gggacatgcc aatctacgta
    19741 gcggaagatt caagtgatat gtgggcaaat ccacatctct tcaaaacaga tgtcaatggt
    19801 aaggctactt gtatcgcagg atgtccacca gatgagtttt ctgtaactgg tcagctttgg
    19861 ggtaacccaa tctatgactg ggaagcaatg gacaaagacg gctacaaatg gtggattgaa
    19921 cgcttgcgtg aaagcttcaa aatctacgat atcgttcgta tcgaccactt ccgtggcttc
    19981 gaatcttatt gggaaatccc tgctggttcc gatacagcag cacctggtga gtgggtgaaa
    20041 ggtccaggct acaagctttt tgcagccgtt aaggaagaac ttggtgagct aaacatcatc
    20101 gcagaagacc ttggcttcat gacagatgaa gtgatcgaat tgcgtgaacg tactggcttc
    20161 ccaggaatga aaattcttca atttgccttc aacccagaag acgaaagcat tgatagccca
    20221 cacttggcac ctgctaactc agttatgtac acaggaacac acgataacaa tacggttctt
    20281 ggttggtacc gtaacgagat tgatgatgcg actcgtgagt acatggctcg ttacacgaac
    20341 cgtaaagaat acgaaacagt ggtacacgct atgcttcgta cagtattttc atcagttagc
    20401 tttatggcaa ttgcaactat gcaagattta ctagaattgg atgaggcagc tcgtatgaac
    20461 ttcccatcta cccttggtgg aaactggtct tggcgtatga ctgaagatca attgacacca
    20521 gctgtcgagg aaggtttgct tgacttgaca acaatttatc gccgaattaa tgaaaatttg
    20581 gtagatttaa agaaataaga caataatcag gagacaacta aacatgttat cactacaaga
    20641 atttgtacaa aatcgttaca ataaaaccat tgcagaatgt agcaatgaag agctttacct
    20701 tgctcttctt aactacagca agcttgcaag cagccaaaaa ccagtcaaca ctggtaagaa
    20761 aaaagtttac tacatctcag ctgagttctt gattggtaaa ctcttgtcaa acaacttgat
    20821 taaccttggt ctttacgacg atgttaaaaa agaacttgca gctgcaggta aagacttgat
    20881 cgaagttgaa gaagttgaat tggaaccatc tcttggtaat ggtggtttgg gacgtttggc
    20941 tgcctgcttt atcgactcaa ttgctactct tggtttgaat ggtgacggtg ttggtcttaa
    21001 ctaccacttt ggtcttttcc aacaagttct taaaaacaac caacaagaaa caattccaaa
    21061 tgcatggttg acagagcaaa actggttggt tcgctcaagc cgtagctacc aagtaccatt
    21121 tgcagacttt actttgacat caactcttta cgatattgat gttactggtt atgaaacagc
    21181 gactaaaaac cgcttgcgtt tgtttgactt ggattcagtt gattcttcta ttattaaaga
    21241 tggtatcaac tttgacaaga cagatatcgc tcgcaacttg actctcttcc tttacccaga
    21301 tgatagtgac cgtcaaggtg aattgctccg tatcttccaa caatacttca tggtttcaaa
    21361 cggtgcgcaa ttgatcatcg acgaagcaat cgaaaaagga agcaacttgc atgaccttgc
    21421 tgactacgca gttgtccaaa tcaacgatac tcacccatca atggtgattc ctgaattgat
    21481 tcgtcttttg actgcacgtg gtatcgagct tgacgaagca atctcaattg ttcgtagcat
    21541 gactgcctac actaaccaca caatccttgc tgaggcgctt gaaaaatggc ctcttgaatt
    21601 cttgcaagaa gtggttcctc acttggtacc aatcatcgaa gaattggacc gtcgtgtgaa
    21661 ggcagagtac aaagatccag ctgttcaaat catcgatgag agcggacgtg ttcacatggc
    21721 tcacatggat atccactacg gatacagtgt taacggggtt gcagcacttc atactgaaat
    21781 cttgaaaaat tctgagttga aagccttcta cgacctttac ccagaaaagt tcaacaacaa
    21841 aacaaacggt atcactttcc gtcgttggct tatgcatgct aacccaagat tgtctcacta
    21901 cttggatgag attcttggag atggttggca ccatgaagca gatgagcttg aaaaactttt
    21961 gtcttatgaa gacaaagcag ctgtcaaaga aaaattggaa agcatcaagg ctcacaacaa
    22021 acgtaaattg gctcgtcact tgaaagaaca ccaaggtgtg gaaatcaatc caaattctat
    22081 ctttgatatc caaatcaaac gtcttcacga gtacaaacgc caacaaatga acgctttgta
    22141 cgtgatccac aaataccttg acatcaaagc tggtaacatc cctgctcgtc caatcacaat
    22201 cttctttggt ggtaaagcag ctccagccta cacaatcgct caagacatta tccatttaat
    22261 cctttgcatg tcagaagtta ttgctaacga tccagcagta gctccacact tgcaagtagt
    22321 tatggttgaa aactacaacg ttactgcagc aagtttcctt atcccagcat gtgatatctc
    22381 agaacaaatc tcacttgctt ctaaagaagc ttcaggtact ggtaacatga aattcatgtt
    22441 gaacggagct ttgacacttg gtactatgga cggtgctaac gtggaaatcg ctgagttggt
    22501 tggagaagaa aacatctaca tcttcggtga agattcagaa actgttatcg acctttacgc
    22561 aaaagcagct tacaaatcaa gcgaattcta cgctcgtgaa gctatcaaac cattggttga
    22621 cttcatcgtt agtgatgcag ttcttgcagc tggaaacaaa gagcgcttgg aacgtcttta
    22681 caatgaattg atcaacaaag actggttcat gactcttctt gatttggaag actacatcaa
    22741 agtcaaagag caaatgcttg ctgactacga agaccgtgac gcatggttgg ataaagtcat
    22801 cgttaacatt tctaaagcag gattcttctc atctgaccgt acaatcgctc agtataatga
    22861 agacatctgg cacttgaact aatactcttc gaaaatctct tcaaaccacg tcagctttat
    22921 ctgcaacctc aaagcggtgc tttgagcaac ctgcggctag cttcctagtt tgctctttgg
    22981 ttttcattga gtataagata caaatttata ctaatacatc ttgtaaaaaa gcgagtttcg
    23041 attgaaattc gcttttttaa atgctgtgga tttgggtcaa tcttgtctaa aaatagggaa
    23101 atcctagata cagtgaaggc tttaaatgct ggtttttact gtcttcagcc ttatattttt
    23161 tcgtagttgg ttacctcata tctcttgtat tcgcttacat aaagtattat aatataattg
    23221 taggaaagaa ggtgttttta tgatatacac acttaaattg gtgttgttta ttacctttct
    23281 tgtaataagc ttgttacctg ataagatttt tggaaaaaat aaaaaaattt ggaaaatagt
    23341 ttttgcaata ttgacggcag tggcagcatt gtcatttatg tactaagtta ttttaagaat
    23401 gtagggaaat aaaccctaca ttctttttag ttttttctgt tttctaaatt ctatttatcc
    23461 aagcgcttca acatttcttg cttcttcgct tcaagttctg cacgcttttc ttcgatttcg
    23521 gcatgttttt tctcgagttc agaacaactt gcatcattgc taaattcttt tcgccatcag
    23581 gagatagggt gagtcgacat gtctattact cacccaaagc agtcctacaa agcaggaatt
    23641 ttctgttact tttttggaaa tagtaacgtt tatacagctt tgacacttcg tatcaaagcg
    23701 ccaaacacac tccgagggat ttacagaaag cagaaaagga atgatctggt ataagatcat
    23761 tccttttctc tctttttctt taagtaatta tatacaatgt acgacgaagt cgtcattgca
    23821 atgctgatcc accacctaaa gggaacttta aacaacattg ataagataaa gaatataaac
    23881 aacgaaaata cgttataccc aattaatttt attgtatatc tcatgattaa aagttaatcc
    23941 ttccgttgtt aggaatggca tcatttttat cccataattg tgctaaataa gtccccggtg
    24001 ataataaatt catagcgaat tctaaagcaa catcatttac aaaccaacta cctagatatc
    24061 tagaaattgc tgaacgaata gcactttttg ctgcatgttt tccttttact ttaattagat
    24121 ttgcaaggcc tgcagtagtt cctcctaatg ctaaagctat tgcagtatct aatagagcac
    24181 ccatttgatt aactgtaata ccttgccaag ctgctctaaa tggagagtat gtaggtggga
    24241 ttgtataatc gccttgtaat tgtcggttaa ttacttcttt gatccattgt tgtgagacgt
    24301 ctggatgaaa agattggatt tcgtttgcaa gtgtattgat ttgttcttct gttagagaag
    24361 tgacaggttg aagttccata tttgtttcaa tttgtgatac ttgttcagaa gcgtatacag
    24421 ctgaaacact tggaatcgct gatacaatta acacaattga cgtcaaaaaa ccgaaataaa
    24481 tttcattaat ttgttcatga gcttttctcc tttttatttg tatctgctta cattttatca
    24541 tatactgtta ttatagtcaa aaaaatatgc tattatgtta aaaaaatatt tttcaaaata
    24601 taaatggacg gatttatttt ggattttatt tgttattttg acctgcctct atattggtaa
    24661 ccatgatttg tttactctca atcatcaaga attctctttt cgtggtagcg tttggggtct
    24721 ggtactggcc ttatatcact tactattcat tgataagttt gttatatcga atcgaaaata
    24781 aagattagag ctatgcttga ctgtgtactt ttaggattga ttttggagga agattttgtc
    24841 tctattattt attattttaa atttatttat tttgtataag atctattctt tgaggcggga
    24901 aaaatcgaaa tacttgattt atacggccta tatcatattt ggggtaaatg tagtatatgg
    24961 tattcaatgg ttattaaaag aactgatttc aactatttcc ccttaatgtg tatggaaact
    25021 ataacaaaac gcataagatt aaggtttgtg tacctgttct tatacgtttt tataatattg
    25081 aagattttcg gtaagtttta tacctttggg aagtctagtt tagaatgctt tcccaaccaa
    25141 aatctctgct tcgatggtaa tctctgtcag ttggctccag tcaatcttgc tctggtcaac
    25201 gtgaaagagg agaggagtca tactgagtag ggcttggagt tgttctgctg tgatagtctt
    25261 agtcagagag gcagtttgac tagataggat ggtaaagtgt tcctggaaat gctccttgat
    25321 atcttggtta gaatactcct tgtttgtcag ctggtcctgt actctttgac ggatttcttt
    25381 gaggtgattt tcagttggga taacctttat caagataccg tctttggata aaacgcgacg
    25441 aaattctcca tagttggcag gtgagaagat atcaagcagg atatccatgt tagcgtcttt
    25501 tataggaagt cgtgccaagt cgccaacgaa ccaattgact gcccagttgg gttcactttt
    25561 agcagcgatt tggactgaat ctttggagat gtcaaaggca tagaaagttt tttcagagtg
    25621 actttcttgt agtttgcgag aatagaatcc ttcaccacaa ccgatatcca aaattgttgt
    25681 ggtagttttt gagcttgcaa gcaagtcaga tacagcatct aagatagctt ggtaaaagcc
    25741 ggcttctagg atttgttgac ggttttgaaa attttccttg tcatagttag cagattgctt
    25801 gatttgaggt gctagattga catagccaaa tttcgccaag tcaaaagaat gacggttgca
    25861 gcacttgaaa ttagtctcta acagagtcag attttcttga cagataggac aggcaaaggc
    25921 agtcgcagaa gcaaaacgct gaagtttggg cttgagattt gtattcatag tttaagtgta
    25981 tcaaaagagg caaatgaaag caactttagg aataagtaga tgggagattc agtagaaata
    26041 aaactaattc tccaatttat agaatgaaat tgcttttata tctaaattgc tatataataa
    26101 tgataaggag gaaataagta tgttaaaaga agtattaacc gttgcaaaag ttgcgaaaaa
    26161 atcttcatta tttttgggtg gtgtcgcatt tggtaccctt ggtttgaaaa tcttagcaag
    26221 taaggaagct aaaaaaggtt attctaaagc tttggctaag gcttacaagt tgaaagacga
    26281 gctagatgca tctgtttctg ttgtgaagca acatggagac gatgtcttgc aagatgccaa
    26341 atatttgtac gagcaagaga aaaaagaaga gcaattagat agccttatag gtgaataata
    26401 tgtcttttaa agtgctacat agaggatacc aacatatccg actatcatct tctttttcac
    26461 tcaccttgga tattcaagac tatcttcgtt ccttggcgag agatgaaaag gggattgagt
    26521 ctatccagtt ttacatggat caacagcact ttactctacg cataaaagaa ggcttttctg
    26581 tattagataa tgcagaagcc tttttaaaaa gaattgataa agggaaagtt tctgagttga
    26641 tgactcttcc cattcgtaga gaagagagtg cttattctat tgtttcaggt gcagcggtta
    26701 agcgtgtact ttttcgtagt tttgtgccgt atcctattcg ctatatatgg acttgttatc
    26761 aggctttggg ttatattaga gaagcctatc aaacactagc gcgtaaggaa ctaacgatgg
    26821 aagtcttgga ctgttcggct attttattgt ctttgtttat gaaccaatcc aagacagcta
    26881 gcaatatcat gtttatgctt gatttgggga atcatttaga tcagtggtcc ttgaaaaaaa
    26941 ctgcaacaga tttagaacag agtcttcttg caaaagagag cgatgtattc ctagtacagg
    27001 gcgatacggt tgttagtatc aagagttccg atgttcaaat aggagatgtc ttgatcttat
    27061 ctcaaggaaa tgaaattctg tttgatggac aagtagtttc aggtttaggt atggtcaacg
    27121 aaagttcctt gacaggagag agttttccag ttgaaaaaag agagtctgat ttggtttgtg
    27181 caaatacagt attagaaact ggagagttac gcattcgtgt aacagataat cagatgaaca
    27241 gccgtatttt acagctgatt gagttgatga agaaatctga agaaaacaag aaaacgaaac
    27301 aacgctattt catcaagatg gcggataaag tcgtcaaata taatttcttg ggggctgggc
    27361 tgacttacct attgacaggt tctttttcta aggctatttc tttcctattg gtcgatttct
    27421 cctgcgcttt gaaaatctct actcctgtag cttatttgac agttatcaag gaagggttga
    27481 accgtgaaat ggtgattaag gatggagatg ttctggagaa atatctggaa gttgatactt
    27541 tcttgtttga taagacagga acaatcacaa ctagttatcc tatagttgaa aaggtgttac
    27601 cttttggaga ctatagtgag gaagatattc tcagaatcag tgcctgtctt gaggaacaca
    27661 tttatcatcc tattgctaat gccatcgtca agcaagctga gatagaggga attgaacatg
    27721 aggaaatgca tgggaaactc caatatatcg caagcaaggg gatcaaatct catatagatg
    27781 gccaaccagt tcttattggg aattatgtct tgatgcagga tgagcagatt catatcagtt
    27841 cagaacaaaa tgctttaatt gaagagtaca agagtcacta caatctctta ttcttggctt
    27901 atcaaaatga attgattgga atgttctgca ttcatactcc tttgagaaaa gaagcaaaaa
    27961 cagccttgga taaacttaag gcacagggga aaaaattgat tctggcaaca ggagacacct
    28021 tgattaggac agaggaatta gtcaaagatt tgccctttga tcaggtctat acagacttga
    28081 aacctgatgg gaaatttgag ttagtagaga aactgcagaa agcaggtcac actattttga
    28141 tggttggaga tggattgaat gactcagcgg ctctaactct atcagatatc ggtgtggtga
    28201 tgaatgagag tgcagatatt tctaagcaga tgagtgatat cttattgtta gataatcgtt
    28261 tggatttctt ccaagagttg gattcgttat catcatcttt gcaaacactc atcaagaaaa
    28321 atattcaaga taccgttgtc gtaaatagta gtttgattgg ctttggctta tttaattggc
    28381 tcagtccttc aaatctctct atcttacata atctaacaac cttgcgcatt gtcctgcgta
    28441 gcctgtctat taaaaataga tagatgagag ttattaacag caaaaaggag ttacctttct
    28501 cgaggttaac tcctttgttt atagataaat gttgaatagt ttagtaagtc aatacaaagt
    28561 atttcttctt cccacgacgg ataacagtca gttcattctc taacttatca gcgtcactca
    28621 agacatagtc aaggtcttgg atgcggtcgc cgtttacgta gatggctccg ttttggacgt
    28681 cttcacgggc ttggcgtttt gagttaacta taccagatga gacgagcagt tccacgatat
    28741 tgttgtgttc gtctgcctgt acttggtagt ttggcacacc acgaagtcct tgtttgagct
    28801 ctttgacaga aaggtttttg atgtttcctg caaagagttg ctcagtgatg ttaagtgctt
    28861 ctttgtaggc ttcttctccg tgaacaagtg taacaacttc acgagccaag actttttgag
    28921 ccaagcgttc gtgtggcgct gcttcaaatt gtttacgaat atcttcaatc tcatcaagtg
    28981 acaagaaagt aaagattttc aagaagcgaa cagcgtcagc gtccatcacg ttcatccaga
    29041 attggtacat ttcgtatgga gaagtctttt cgggattgag ccagacggca tttccttctg
    29101 atttaccaaa ttttttacca gttgcatctg tgattagtgg aacagtgata acgtgaccag
    29161 tcttgtccgc cttacgacga agcaattcgg taccagctgt catatttccc cactggtcag
    29221 aaccaccgat ttgaagagtg acattatggt cttggttaag gacgaagaag tcgtaccctt
    29281 gcatgatttg gtaagcgaac tcagtgtaag aaattcctgt ttcgatccgt tttttaacag
    29341 attccttact catcatgtag ttgaccgtga agtattttcc aatgtcacgg aggaagtcaa
    29401 tgaagctgat gctgccaaac cagtcgtagt tgttgaccat gacagccttg ttttcgccat
    29461 tttcaaagtc aagaaaacga gaaagttgtc cttggataga cttgacccag ccatctactg
    29521 tgtcttttgt ttggagacta cgttcagcat ctttgaagga cggatctccg atgagacctg
    29581 tagcaccgcc aacgagcgca taaggtttgt gacctgctag ttgcaagcga cgacttgtca
    29641 agattgcgac aaggtggcct aggtgaaggc tgtcagcagt tggatcgtag ccagtataat
    29701 aagaaacttg accttcttct agggctttac gcaaagcttc ttcatcagtc gtttgaaata
    29761 tcaaaccacg ctcttttagc tcatcaaaaa tgtgcatgtg tcttttctcc tttttaaaat
    29821 tattgtttct acctattgta tcacaaactt gggcaatagc ctagcagaat agggaagaaa
    29881 gtggctattt tattgaaaat ttcccttttg tggtataata gatagagtga ggaaatccat
    29941 gcaaaatcaa ttaaatgaat taaaacgaaa aatgctggaa tttttccagc aaaaacaaaa
    30001 aaataaaaaa tcagctagac ctggcaagaa aggttcaagt accaaaaaat ctaaaacctt
    30061 agataagtca gccattttcc cagctatttt actgagtata aaagccttat ttaacttact
    30121 ctttgtactc ggttttctag gaggaatgtt gggagttggg attgctttgg ggtacggagt
    30181 ggccttattt gacaaggttc gggtgcctca gacagaagaa ttggtgaatc aggtcaagga
    30241 catctcttct atttcagaga ttacctattc ggacgggacg gtgattgctt ccatagagag
    30301 tgatttgttg cgcacttcta tctcatctga gcaaatttcg gaaaatctga agaaggctat
    30361 cattgcgaca gaagatgaac actttaaaga acataagggt gtagtaccca aggcggtgat
    30421 tcgtgcgacc ttggggaaat ttgtaggttt gggttcctct agtgggggtt caaccttgac
    30481 ccagcaacta attaaacagc aggtggttgg ggatgcgccg accttggctc gtaaggcggc
    30541 agagattgtg gatgctcttg ccttggaacg cgccatgaat aaagatgaga ttttaacgac
    30601 ctatctcaat gtggctccct ttggccgaaa taataaggga cagaatattg caggggctcg
    30661 gcaagcagct gagggaattt tcggtgtaga tgccagtcag ttgactgttc ctcaagcagc
    30721 atttttagca ggacttccac agagtcccat tacttactct ccttatgaaa atactgggga
    30781 gttgaagagt gatgaagacc tagaaattgg cttaagacgg gctaaggcag ttctttacag
    30841 tatgtatcgt acaggtgcat taagcaaaga cgagtattct cagtacaagg attatgacct
    30901 taaacaggac tttttaccat cgggcacggt tacaggaatt tcacgagact atttatactt
    30961 tacaactttg gcagaagctc aagaacgtat gtatgattat ctagctcaga gagacaatgt
    31021 ctccgctaag gagttgaaaa atgaggcaac tcagaagttt tatcgagatt tggcagccaa
    31081 ggaaattgaa aatggtggtt ataagattac tactaccata gatcagaaaa ttcattctgc
    31141 catgcaaagt gcggttgctg attatggcta tcttttagac gatggaacag gtcgtgtaga
    31201 agtagggaat gtcttgatgg acaaccaaac aggtgctatt ctaggctttg taggtggtcg
    31261 taattatcaa gaaaatcaaa ataatcatgc ctttgatacc aaacgttcgc cagcttctac
    31321 taccaagccc ttgctggcct acggtattgc tattgaccag ggcttgatgg gaagtgaaac
    31381 gattctatct aactatccaa caaactttgc taatggcaat ccgattatgt atgctaatag
    31441 caagggaaca gggatgatga ccttgggaga agctctgaac tattcatgga atatccctgc
    31501 ttactggacc tatcgtatgc tccgtgaaaa gggtgttgat gtcaagggtt atatggaaaa
    31561 gatgggttac gagattcctg agtacggtat tgagagcttg ccaatgggtg gtggtattga
    31621 agtcacagtt gcccagcata ccaatggcta tcagacctta gctaataatg gagtttatca
    31681 tcagaagcat gtgatttcaa agattgaagc agcagatggt agagtggtgt atgagtatca
    31741 ggataaaccg gttcaagtct attcaaaagc tactgcgacg attatgcagg gattgctacg
    31801 agaagttcta tcctctcgtg tgacaacaac cttcaagtct aacctgactt ctttaaatac
    31861 tactctggct aatgcagatt ggattgggaa gactggtaca accaaccaag acgaaaatat
    31921 gtggctcatg ctttcgaccc ctagattaac cctaggtggc tggattgggc atgatgataa
    31981 tcattcattg tcacgtagag caggttattc taataactct aattacatgg ctcatctggt
    32041 aaatgcgatt cagcaagctt ccccaagcat ttgggggaac gagcgctttg ctttagatcc
    32101 tagtgtagtg aaatcggaag tcttgaaatc aataggtcaa aaaccaggga aggtttctgt
    32161 tgaaggaaaa gaagtagagg tcacaggttc gactgttacc agctattggg ctaataagtc
    32221 aggagcgcca gcgacaagtt atcgctttgc tattggcgga agtgatgcgg attatcagaa
    32281 tgcttggtct agtattgtgg ggagtctacc aactccatcc agctccagca gttcaagtag
    32341 tagttctagc gatagcagta actcaagtac tacacgacct tcttcttcaa gggcgagacg
    32401 ataattctct aaatgaagtg gccaatcaat ggattgccac ttttttcctt tcgtgttaca
    32461 ataagggtat gaatcagtat cagaaaaaga ttgttaacgg aaaaatttat tcgctcctat
    32521 ccggcttaat atggggaatc tgtggaattt taggagagta cttctttact cattatcagg
    32581 tgtcttcggg ctggattacc tctatgcgtt tgacactggc agggagtctt gtactcattt
    32641 ggtctgcaat acaattaaaa tcgcaagtgc tagatatttg gcgagacaag aaaaattacc
    32701 tgcccttttt agcctatgct attttgggga ttttttcagt tcagtatttt ttctatctct
    32761 gtgtagaata ctcaaatgct acgacagcaa ctattttaca gtttattagc cctgtcttta
    32821 tcctctttta caatcgcttg gtttatcaaa aacgagcgtc aaaaagcgct gttttctatg
    32881 ttttggttgc catgctgggt gtttgcttga tggcgacaaa gggagatctc tctcagttat
    32941 ccatgacgcc gctagctctt ataacaggtt tgctgagtgc catgggtgtt atgtttaatg
    33001 ttatcttgcc ccaacctttt gctaagcgtt atggttttgt tcctacggtt gggtggggga
    33061 tgattttggc aggtttgttt agcaatgtcc tctcgccggt ttatcagctt tcctttactc
    33121 ttgatatttg gagtatcttg atttgcctca ttatcgcttt ctttggaacg gcttttgctt
    33181 ttttcatttc catgaaggct gtgtccttgg tttctccttt ggtggttgcc gttatcagtg
    33241 ccagtgaacc tctctcttct gctctcttga gtgttttgtt cttaggatta gtagtggatt
    33301 ggtccctcct tctagctata gccttgatta ttttacccat gatatttttg tctatagaag
    33361 aagcgaaaga aagtagataa aaagtctttt cttaattcta aaagtgtgct atactagaat
    33421 agtcaataaa acacggggag atagttgtga agagtgtttt taggttgtat cggtaggggc
    33481 ttgcttttac cgacagcacg ttttatctca catctctaaa aaatcatacc taaaaacaga
    33541 caaaaaggtt tcaaatttga ggcctttttt ggtagaatag gtatcattat aacgaaccag
    33601 gaggcaccta tgactgctac aaaaatgaac gctcaagaaa ttatccaatt tatcgccaat
    33661 gctgaaaaga aaaccagtgt caaagtaacc tttgaggggc aactcgcaac tgctgtgcct
    33721 agctctgttg tcaaactagg aaatgtccta ttcggagact ggaaggacgt ggctccgctt
    33781 cttgaaggtt tggtagaaaa tcaagattat gttgtcgagc aagatgctcg taattctgca
    33841 gttcctttgc tagataagcg tgctatcaac gctcgtatcg agccaggtgc tattatccgt
    33901 gaccaggtgg aaattggtga caatgctgtt atcatgatgg gagctgttat caatatcggt
    33961 gctgaaatcg gtgctggaac catgattgac atgggtgcca tccttggtgg ccgtgccatc
    34021 gttggaaaaa atagccacgt tggtgcaggt gcagttttgg caggtgtgat tgagccagct
    34081 agtgctgaac cagtccgtgt cggagacaat gttcttatcg gtgctaatgc agtggttatc
    34141 gaaggagtcc aaatcggtag tggttcagtt gtcgcagcag gagctattgt tacccaagat
    34201 gtcccagaaa acgtggtagt agcaggtgtt ccagctcgta ttatcaaaga aattgatgcc
    34261 caaactcaac aaaaaacagc gctagaggat gcgcttcgta ccttgtaatt gtaaaagtaa
    34321 aaaagaggcg gaaccctttt tccagcctct ttctgctata tcggaggaca gatagatgtt
    34381 agatttgatt cagactagac gagctttaca ccagatacca gagattggct tggaggagtt
    34441 caagactcag gcttatttgc tggatgtgat tgagaaattg actacgggca aggattttgt
    34501 tcaaattcgt acttggcgga cagggatttt ggtctacctg cagggaagtc agccggagcg
    34561 aaccattggt tggcggacag atattgatgg cctgcctatc gtcgaacaaa caggtctacc
    34621 ttttgcttct caacaccaag gtcgcatgca tgcctgtggt catgattttc atatgaccat
    34681 tgccttaggc tgtcttgagc gcgcccttga ggagcaaccc aagaataatc tgctctttct
    34741 atttcagcct gctgaagaaa atgaagctgg tggtatgctc atgtatgagg acggtgcttt
    34801 tggagattgg ttgccagatc aattctatgg tctccatgtt cgtccagatt tgaaagtcgg
    34861 ccagattgcg actaatactc atacactctt tgcaggaact tgcgaggtga agatccgttt
    34921 caaaggcaaa ggtggccacg cagcttttcc tcatgaagcc aatgacgcct tggtggcggc
    34981 tagttacttt gtgacccaag tgcagtcagt tgttagccgc aatgtcaacc caatcgaggg
    35041 agcagtggtg accttcggcg tttttcaagc tggaaccacc aacaatgtca tcacagacac
    35101 agctttttta catggaacta ttcgtgcctt gacacaggac atgagtcttt tggtgcaaaa
    35161 gagggtaaaa acagttgcag aaggggttgc agctgccttt gatatggaag tcgaagtgga
    35221 actcaaacaa ggtggatacc tacctgttga gaacaatcca gccttggcgc gtgaactgat
    35281 ggacttcttt gacgaaaaag acggaatcga gttgattgat atcgaacctg ctatgacagg
    35341 cgaggacttt ggttatctcc tttcaaaggt tgatggcgtg atgttttggc taggtatcga
    35401 tagtccctac gctcttcatc accctcaaat gagtcctaag gaagaagtct tagctattgg
    35461 ggtggctgcg gtgtctagtt tcctgaaaaa gaaggcagca gaatagaggg cttgtctatg
    35521 aaatcggaat tacgcaagca agtcttgcat gaaatgaagg ctttatctca agaacaaaaa
    35581 caggctatag accaagcttt aaccgagcga attttacaac acccctttta tcaagaagcc
    35641 aaggtcatcg caacctacct ctctttttct catgagtttc aaacgcggga actgattgag
    35701 caggcgctga aggacggcaa gaaggtttta atacccaaaa cttatcccaa ggggcgcatg
    35761 gactttgtag tctatgatcc gcagcagttg gtaaaaactg cctttgcctt actggagcca
    35821 cagggagatt tggaagtggt ggatgtatct cagattgatt tgattcatgt tcctggcctg
    35881 gcttttacga cggatggata tcggattgga tatggtggag gttattatga ccgctatctg
    35941 gaacattttt ctggtcatac tttgagtacg gttcatcctt gtcaaattca ggactttata
    36001 cctgaaaacc atgatattcc tgttcaggag gtattaattg atgaaggaaa tctttgatag
    36061 acgttaccct gtgacgagtt tcttcctctt agtgacggcc ttggtatttt tactaatgtt
    36121 ggtcactgca ggcggaaact ttgacagggc agatacatta tttcgatttg gagccatgta
    36181 tgggccagct attcgcctct ttcccgagca ggtttggcgt ctcttgtctg ccatttttgt
    36241 tcatattggg tgggaacatt tcattgttaa tatgctttca ctttattatc ttggaaggca
    36301 ggtagaggag attttcggct ctaagcagtt cttttttctc tatctcttat caggaatgat
    36361 gggcaatctc tttgtttttg tatttagtcc taaatcctta gcagcaggcg cctctacctc
    36421 tctttatggg ctatttgccg cgattattgt tcttcgctat gcaactcgca atccttatat
    36481 ccaacagcta gggcaatcct atctgacact ttttgtggtt aacattattg gaagtgttct
    36541 gattccagga atcagcctag caggccatat cggtggtgca gttggtggcg catttctagc
    36601 agttatcttt ccagttagag gagaaaaacg gatgtataat accagccaga gattaggagc
    36661 ggtagtcttg ttcgtaggac tcgccatttt gcttttctac aagggaatgg gaatgtgacg
    36721 aagttgaaag aaaatttgtg taatgaaaaa agataaggca ttttttgaac cttatctttt
    36781 tattttattc cttctcagtc aattcttttc caagttggat gaggtaattc ttcaaatcat
    36841 ctttgacttg tgggtgtttg agggcgtagt caatagatgt tttcataaag ccaaacttat
    36901 ccccaacgtc gtaacgagct cctgtaaatt cacgggcaaa aacacgctgt gttttattaa
    36961 gagtatcgat tgcatcagta agttggattt catttccagc accaggagct tgcttttcga
    37021 gaatctcaaa aatttcaggc gtgaggaggt agcgtccgat aatagcaagg tcgctaggag
    37081 cgtcctctgg agctggtttt tcaacaaagg tttcaacact gtaaagacca tcttttcctt
    37141 cgccttgcgg agcaataacc ccgtaagcag atacttcgtc atgagggact ggcatgacag
    37201 cgatagtaga cgcgtgggta cgcttgtagt catctatgag ttgtttggta agtggaacag
    37261 ccttttcgtc tgtgatatcc atcaagtcat caccaagcat aacgacaaaa ggttcatttc
    37321 cgacgaaagc cttggcttgc aaaacagcat ctccgagacc gcgtggatga gtttggcgga
    37381 taaaatgcag acgcatgcca gttgtttcat caactagctt caaaagatct gttttccctt
    37441 tttctttgag gttatattcc aattcgaagt ttgaatcgaa gtggtcctca atagaacgtt
    37501 ttgatttacc agtgacaact agaatatctt caatacctga tttgagagcc tcttccacga
    37561 taaactggat agttggtttg tctacgattg gcaacatttc tttggcaagg gccttggttg
    37621 ctggtaaaaa tcgagttcct agtccagcag cagggataac tgcctttcta acttttgatg
    37681 tcataaaaat cctttctata gagggttaag accactcatt ttctgcttta aattcattgt
    37741 tcatgatgtc ataaatggca tctttgatat tggttccgtg ataaataact tggtaaatag
    37801 cctgtgtaat gggcatatag actccaagtt cttgggctag ttcataggct gctcgagtcg
    37861 ttgaaattcc ttcgattacc atgcccatat tagcttctat atcagctagg gattctcctc
    37921 gtccgagagc atctccagct ctccagttac gagagtggat ggaagttccc gttacgatca
    37981 aatctcccac accagataag ccgctatagg tcaatggact ggccccgagt gctaccccta
    38041 ggcgggtgat ttctgctaaa cctcgagcga tgatggctgc cttagcatta tcaccaaatc
    38101 caagaccatg taaagctcca gcaccgacag caataatatt tttaagagca ccagcagttt
    38161 caaccccgat aacatccgta ttggtataaa gtcggaagta gtgattacta aatagctcct
    38221 gaacgtattg agctgtttgt aaatctttag aagcagcagt tattaaagtt aggtcacgca
    38281 caatggtctc ttctgcatga ctaggccctg aaacaacgac gatatcacta cggagatgtt
    38341 caggaatttc ttcttcaaga atggttgata atcgtttatg gctatcaggt tctaatccct
    38401 ttgatgcgtg catgatgata accttatggt ccaaggtttg tgcaacttgc tgggcaacaa
    38461 gtcgtgtcac ttttgttggg acaacaaaca aaatcgcatc cacatttttc aatgtttctg
    38521 ctaagtcggt gtaggcaatg atattttcgt ctagaacgac atctttaaag tagtgcttat
    38581 tagtatggtg tgtattaatt tcattgattt gctcgggaag atttccccaa atacgtacct
    38641 cgtgtccatt gtcatttaag acttgtgaaa gggcggttcc ccaagaacca ggccccaaga
    38701 cggcgacggt ttgtttttcc atgatttcct ccttgataga tttctttctc ttattctatc
    38761 atattctagg ggattttgca cttgcattac tggggtatgg tggaaaaata cacaaaaacc
    38821 gagacgaagg gataaactct tagtctttag ttaaagtgag aagaaatctg atagatagtt
    38881 tgattcagta tatgcttata atagaggtaa acaactcagg aagttcttgt aggttgcgag
    38941 agtttggcta aaaaaatcag tcgttcagaa ttttgtggtg atgagtctga attaagtatc
    39001 cgtcaattaa ttagaattga aaatggagaa tccagaccaa cactaacaaa gttaaaatat
    39061 atcgctgaac gtttgggggt tgaagattac aagttgatgc caagttatat agagttggat
    39121 aaggaatacc tagaattgaa gtatttcttg atgaggactc ctacatacga agatgaaact
    39181 atcgcccaaa agaaagagag tgttttgata agatttttga agagtattat gataggctac
    39241 ctgaggaaga aagatttatc atcccaaatt attcatatct ggcactaacg aactacacag
    39301 ttcaaaaatt accagaaaag ctagttgaaa tactgtcctt ctggtgattt tgcttaattt
    39361 ctgatgttcg agacatgatt gaggagattt gtcttgattt tttcctaaaa aagggcagac
    39421 tcctcccttg gtttgatgct cgatttttaa aataaggtga cttattctgt taggcatgtt
    39481 tccccctgcc ctctagctct atatggaaca gggagtatca gatttatctg gcagattgta
    39541 tcaaataagg cttgaaactg ttttgaatag gcgtatagac ttgagaattt gagaatatgt
    39601 cgtctagcag tagagacata ttttccggca atatgaagga agagacgtcg aaaacgcttg
    39661 atagtcaagg cctttacttc atcaccagct agctgcttta aaaagaggta gagattataa
    39721 gccagacagc ccatcatcat acgtacttca ttcttaatca tggtcgaact atctgtctta
    39781 tccccaaaga atcctgcttt cctttctttg ataaagtttt cagcattccc tcgttcacga
    39841 tagaattgaa acagctcctc actatctaca ctgcttaaat tgttcaaatg aactttttta
    39901 caaccataaa aaagagcact taatcgtact cttctctttt aaggattagt tgaattaatt
    39961 caaatccact agttctcctg tttctaggta gactaggcgt tcacaaatgt tagcaatgta
    40021 atcagcgaag cgttccagat gccctatgat ataaaggtat tgagttccat tgggaattga
    40081 ggtttcttgg tctttcataa gtccaatgat ttcctttgat aaggcataat aatattggtc
    40141 aatctgttca tctttttgag caatactaat agcttttgag gcttggtgca aaggaaaggc
    40201 aaccaataaa tcggctagca tgctgaggga taatttaccc atttggtgta actgttcttc
    40261 gtcaggggct agttgatttt cttttagttg caaaacagct ttggcaatgc ctgccatatg
    40321 gtctcccata cgttcaaggt ctgaacaaga agacatgatg ctaatcacaa atcgaaggtc
    40381 agacacttgt ggctgctgca aggccaacaa acgggcacag gtcaattcga tagcgctttg
    40441 accttggttg atagcatgat ccttattgat aattagctct gccatctcct tgtctttgga
    40501 ggctaaggcc agtaaggctt ttgaagctgt ttcaaggaca agttgcccta gtcctaaaaa
    40561 ggattgttct aattcatgca attctaagtc aaattgattt ctcataggtt tttccttcct
    40621 tatccgaacc gtcctgaaat atagtcttct gtgcgctgat cttttggatt ggtaaacacg
    40681 tcaacggtat ctccaaattc gcaaatttct cctgttaaga aaaaagcagt tttatctgaa
    40741 atacgtgaag cttgttgcat gttatgggta acaatgataa tcgtataatc cttttttagt
    40801 tgctgaatga ggtcttcaat ttttaaagtg gagatagggt ctaaggctga agtcggctca
    40861 tccattaaca gaatatcagg ttctactgct aaagctcgcg caatgcaaag gcgttgctgc
    40921 tgaccgccag ataaggacat ggcactcttt ttaagatcat ctttgacttc ttcccaaatg
    40981 gctgcccctt ttaaagattt ctccactaag gcatctaatt gttttttgtc tcgaatacca
    41041 tgtgtccttg gaccataagc cacgttatca tagatagaca tggcaaaggg attaggctgt
    41101 tgaaaaacca tccctacacg cttgcgtagc tgattaaggt tgaatttgct actataaata
    41161 tcttgctcat ctaagaggac ttggccttca atatggcaag aaggaaccaa atcgttcatc
    41221 cgattaaggg tttttagaaa agttgatttg ccacaaccag atgggcctat caaggcagta
    41281 atctgtcttt ctggtaattg aatcgaaata ttttttaagg cttgaaaatc cccgtaaaat
    41341 aagtctaggt gtctaactga aaatgttccc atactaggaa gctcctttca caagtttccg
    41401 agataataag cttgatagag tatttatcat taaaacagta ataatcaaaa tcacgccggt
    41461 agcataggct tcattgacat gtagcccctc acttgacagc atatacatat gtagggctag
    41521 agaacggcct gaagacatga gactacttgg cgtattggta gaggtaccta atgtatacat
    41581 gagggcagct gtttcaccaa cgatacggcc aatagctagt atcactccag ctaaaatacc
    41641 tggcatggca actggtagaa caattctaaa aacagtccgt aacttacctg ccccaagtcc
    41701 ataacttgct tgacgcatgc tatcactaac agataaaagg gcttcttctg ttgagcgaat
    41761 aatgactggc aacaccatga taactgaggt taagattcct gataacagag agtattgaaa
    41821 acctaagaag actacaaaga agagcatgcc aaacagacca aaaacaatgg aaggaatccc
    41881 agacaaggta tctgaggcca atcgcatgat tttaacacaa agggaatctt tttttgtata
    41941 ttccacaaga taaaaaccag caaaaatccc tatgggcaag gctaaaagaa gagcaccaaa
    42001 gaccagaata acggtggaaa taatcgctgg cataagggaa atgttctcag aagtataagt
    42061 ccaagaaaag agggatagac ttagatgagg taagcctttg atgaggataa aaccaatgat
    42121 taaaaagaga gagccaaagg ttaaagctga aaaacaataa acgagaagtt ttagcaggta
    42181 tttactcata agatgatttt cctttcaagt aggcaaagta ggcattaatc aagagaataa
    42241 ggaaaaagag aactgctgag gttgcaataa gggcttccct atgctgacct gatgcgtaag
    42301 ccatttccag aacaatattg gttgttaagg ttctggttcc tgaaaagagt ccacttggaa
    42361 taatcggctg gttgcctgcc accaaaataa ctgccatggt ttcacctact gcgcgaccga
    42421 ttcctaaaat aactgctgat aaaataccag atctcgcagc tggcaagatg acactaaaaa
    42481 tactccgttc atgactagct cctagagcca agctaccaga ataatacgtt ttgggaactg
    42541 ttcggatagc agattctgac aaactgataa tggttggcaa aatcattatt cctaatagta
    42601 acgaagcggt taggacactc atgccatttc ctaaaaagct tctaatccaa ggcaccaata
    42661 attgtaggcc gaaaaaacca taaacaatag atggaatggc tgccatcaag ttgatagctg
    42721 attttaagaa gccatagacg ggctttggac aataataaac cataaacacc gatgtcaaga
    42781 tgcctgttgg caccccaatc acaatcgctc ctaaggtaat taataaggaa ccaacgatca
    42841 ttggtaaaat accatagctt gccggaatgt tcgttggcga ccaatcactg cctaataaaa
    42901 aacgggcaaa gccgtagtta gctatgaaag gtaagccatt actaaaaata aagaaacaga
    42961 ttagcaaaat agctacaaca gctactgttg cactcatgaa aaaaattgcc ctaaaaactg
    43021 cttctttgaa ggcttgtttt gtcacatctt gtcctttcta gtgaagaaag taagggagat
    43081 acgacacctc cctacttgcc ttctttatct tattgtacga tgaaacgtct gcatctcttt
    43141 agagatttat ggagcaaaca ttttatttaa tcttgtccca ggtggttaat ttgccactaa
    43201 aaacgtctgc aagttcagcc atactgactt ggcttgcctt attgtcatta ttgaccacaa
    43261 cagcaatacc gtctaaagca atagcatcat gggtgagact cttaccttct tcaggagtta
    43321 attccctaga aaccatacca atatcagcgg ttttctcctt aacagcggta atacctgctg
    43381 aagacccatt agaggtaata tcaatcgtaa cttctggatt ttctttttta taagcttctg
    43441 ctaatttttc cattaaagaa gatactgaag tggaacctac aacagacaac ttgcctgata
    43501 agtgttggct tgtatattct gtggtttcgg ttttagcttc aataaattta ttatctgtga
    43561 ccacttgttg accttgtttg gagtggataa agctgataaa atcttgacct agcttggaaa
    43621 gattagaaga ccaaacaatg ttgaagggac gttgaagagg gtattcacca tctaaaactg
    43681 tgtctcgact agccttgaca ccatcaatct ctaaagcctt gacagatttc gttaaagatc
    43741 ccaaggagat gtagccgata gcattagcat tcccttgaac tgctgagaga acaccttctg
    43801 tactattttg aatcacagct gttttggcag tgttgtcaat ttttttatca ccgtcttttt
    43861 tgagaatacc tgtgatttct gtgaaggcac cccgtgttcc agagccattt tctcgtgaaa
    43921 tcacctcaat cgttcctgaa gctgactgtt tggaagcagc tgactgattg ccacaggcaa
    43981 caagcccaaa tcctgataag ccaatggctg caagagtaag catttttttg aatttcataa
    44041 taatcacctt tatctctatg tatttttctt gtgtaggctt actacattta tagtctaaca
    44101 agtctttgta aaggtttatc cctgattcat gtaaagattg tgtaaagaat caaaaaaagc
    44161 cacttttgaa aaatggctgc tcctaaaaat agctttaaaa attattagtc ctgtgcgaaa
    44221 gattggttag gaagaaaaat cgtgaagcaa ctgcctctgc caagctgact cgtcaccgtg
    44281 acttggccac ctaataattg actgagttct ttgacaatgg caaggccaag accagtgcca
    44341 ccagtttgtc tgcttcgacc tttattaact cggtaaaaac gttcaaaaat acgatcctgc
    44401 tctaattgac taataccaat ccctgtatct gatacagaaa tcttaatgcc ttcgttcacc
    44461 ttttgggtct tgacctcaat tttcccccct tgttcagtgt aacggatggc attggataaa
    44521 agattgagta agatttggga aagtaattga ctatctgata cgagggtgac atcatctggc
    44581 acctgcacct ttagctgtaa atccttcttc ttgagctgag gttgcaagct ttgagtcaaa
    44641 tcctgtacaa attctgccaa agaaagggtc gtccattgta taggcatttg ttgagcctta
    44701 gataaggtaa gaagatgctc aacaatatgc tcaagacgca aactttcttt gtaaataatg
    44761 tctagaaagt catccttgag cgcttcttct tcagctgaca tccccttaat ggtttcagca
    44821 aagcccttaa tcgaagtaac tggtgtcctc aattcatggg aggcatttga gacaaaggct
    44881 aaatttaact tttcataagt tctaatcgtt gttaaatcat atagcaagac gagcacagct
    44941 tccacagatt gggtggggct aaaaacggga actgctgtca cttctaaaat caagtcaccc
    45001 tcatgaaacc cacttacttc ttgttttaac cttgtttttt gatcaaaggc ttggtgaact
    45061 aaattccgaa tatccatccg tttgaggtca tcaagtgaac ttatgtcgcc gtccacatcg
    45121 ggaaaataat gaggcagaga gcgactggat aataacatct gaccttgagc ggaaactaaa
    45181 aacgtcccca tggttaggtg cgacagaaga acctccattg tttcggctag atccttgtat
    45241 tgctgatcct gttgggagac tttggttttt aggccagaca catactgagc caaagacttt
    45301 aagtcttctt gccctttttc taaaaagtat tcactactgg tcaagagagg ttggtgcaag
    45361 gtctcaaaag caacttccca tttccaaagg caaaagagcc agtagccacc tagtcccaaa
    45421 gaaagggcta gaagaaagag accgatgcct ttactgatcc aagttaatgc catccctgca
    45481 atcagaatga ggctaacact tagattgact agccaaaatt gaaggtagcg tttcatctat
    45541 aactccttga acttataacc ataaccccga atggttcgaa taaattgagg ggctttagga
    45601 ttgtcttcaa ttttttccct caacttacca atatgaacgt ccaccaaacg tgtttcctgc
    45661 ccaaagtcat acccccagat acgttccaaa agacgctctc tagtcagtgt catgttggga
    45721 tgtttcataa gatagagcaa gagttcaaat tcttttgggg tcaaactcag taacttattc
    45781 gccttgtaga cttcatgacg ctcagggtat actttcaagg tcccaaatag ccaagaatcg
    45841 tcagcgatat tatctgaatc atctccttct tgttctcctt tagttcgcct gaggacagcc
    45901 ttgacacgcg ccagcaattc tctagggcta aaaggcttgg tcaggtagtc atcagcccct
    45961 aattccaagg ccaaaacctt atcaaattca tcacttttcg cagaaaccat cataattgga
    46021 gttttgacgc ctttggctct cagccgctta caaacttcca tgccatctaa ttgtggtaac
    46081 atgatatcaa gcaagataaa atcaaagggt tctgtttctg ccaaagctaa ggccttccgt
    46141 ccatttgtta ccaattgagt agaaaagcct tccttactta aatggtagtc aagcaatttc
    46201 agaatgtgtt cttcatcatc cactaataag acttgttttg tcatctatta tctcctattg
    46261 gtaacattat aacacaatta tcagaaatcc taacattgct aaatcagatt aaatttgctt
    46321 atcaagacta gtatctggtc aaacgctcaa tcatctcctt gtgctctgga taggtcgcca
    46381 gtagatctac cctttcaaat aattcaaaat cctcaaattc aaaaccagga gcaacaagac
    46441 aagaaaccag agcataatcc ttatcaactg ttgatcccca aatagtgccc ttaggaacac
    46501 agtagtgaag ttgttgccct ttggatatgt ccaggcctaa agtgactact tcgtagtgac
    46561 catctgctgt aatcatgtga acagtaagtg gggatcctgc atgaaaatac cagatttcat
    46621 ctgctgtcaa tcggtgaaaa tgtgaaggat tcgtttcttc ttaataagaa ataaatactg
    46681 gtataaagcg cccttccctt accagcaagg tttatagtgt ctgaagcttt ttttgtttgt
    46741 ctaaaatagc caccttcaac atggggagct aactctagag ttcttatcaa gtcttcttta
    46801 tccgtcggag ccaatgggtt gaagtaactc ttgttcaaag tggtgttacg atttcaagaa
    46861 ctcctctcag ctctgaggac acggtaatga ttgatgcgac ggaagtacaa atcaatcgcc
    46921 ctaaaaaaag aattagcgaa tcattctggt aaaaagaaat ttcacgctat gaaggctcaa
    46981 gcgattgtca caagtcaagg gagaattgtt tctttggata tcgctgtgaa ctattgtcat
    47041 gatataaagt tgttcaaaat gagtcgcaga aatatcggac aagctggtaa aatcttggct
    47101 gacagtggtt atcaagggct catgaagata tatcctcgag cacaaactcc acgtaaatcc
    47161 agcaaactca agccgctaac agctgaagat aaagcctgta accatgcgct atctaaggag
    47221 agaagcaagg ttgagaacat ctttgccaaa gtaaaaacgt ttaaaatgtt ttcaacaacc
    47281 tatcgaaatc atcgtaaacg cttcggatta cgaatgaatt tgattgctgg tattatcaat
    47341 catgaactag gattctagtt ttgcaggaag tctattattt ggttaggtga attagtgaag
    47401 tgtttaggca agtgtctctg gttacgacgt catggcctct aaatcgatta tatttagggg
    47461 tcatgactag tgaagcagtt agctagttcg catataagcg gctagcgtct aacaattagg
    47521 aactttagtt ccaataactt taagattacg acgttttagg acataaatcg atcatattta
    47581 tgtcctaaaa ctagtgaagc gcctagccaa agtccgaata ggatttggcg ttagttactt
    47641 agattgcttt gcaatcaagt aactttggcg atttacatct tctctggcgc ttctactcca
    47701 agcaagcgaa gggcttcttt gagaacgact gcggttgcgt agctgagggc tagacggctg
    47761 tcgcgttctg ggctttcatc caagatacgt gtatgtgcat agtatttgtt aaaggattga
    47821 gccaggctaa ttgcaaattt agcaatgata gaaggttcaa agttatctgc cgcacggttg
    47881 ataatacgtg ggaagtcttg aatgagttta atgatttccc agctttcagt atcattcaag
    47941 ctatagttgc cagctgtttc tggtttgaaa tcggctttgc gtaagataga ttggatacga
    48001 gcgtaggcat attgaacgta aggtccagtt tcaccctcga aggataccat agcttctagg
    48061 tcgaagtcgt atccatttgt acggtcggtt ttgaggtcat agaatttaat ggctccaacc
    48121 ccaacagcat gtgctacttg gtctttgttt tctagttcag gatttttagc ctcgatttgg
    48181 accttggcac ggctaacagc ctctgcaaca gtaggctcta gcaagatgac attcccttta
    48241 cgagtagaga gcttcttccc ttcttttgta accagaccaa aaggaacgtg ggtaatgtcg
    48301 tcactccaat cgtagcccat ctcttgcaag acagctttga gctgtttaaa gtgggcagat
    48361 tgttcttgac caacgacata gatagattta gcaaattggt attcgttttt acggtagagg
    48421 gctgcagcca agtcacgtgt gatatagaga gttgcaccat cagacttctt gatgagggct
    48481 ggatgttcaa ttccgtattt ctcaagattg acaacttggg caccttctga ttcaagaagt
    48541 agtccttttt cagaaagaat gtctacaact gcatccatct tatcattgta gaaggcttct
    48601 ccgttatagc tgtcaaattc aaccttcaat tcattgtaaa ggcggttaaa ttccactaaa
    48661 ctttcatcgc ggaaccattg ccaaagagcg agagcttcct catctccatt ttcaagttta
    48721 cggaaccatt cgcgcgcttc ttcatccaag ctagggtcat tttcagcttc agcgttgatg
    48781 cggacataga gtttaaggag ttcatcgatt ggatgagctt ttacagcttc ttcgtcgccc
    48841 cattttttgt aggcaacaat caacatccca aattgtttac cccagtctcc caaatggttg
    48901 accttgaccg tttgataacc gattttttgg aaaatatgtg acaagctatc tccgataaca
    48961 gttgaacgca ggtggccaat agaaaatggt ttagcgatat tcggactaga catgtcgata
    49021 acaacatttt cttgtttgcc aatattttgg tcagcatagt gttctttttc agtggtaaca
    49081 gcttgcaata cttgagcaga aatggcagat ttatcaagga aaaagttaac gtaaggtcct
    49141 gttgcgacaa ctttttcaaa ggcttggctg ttcatttttt cagccagttc agccgcaatc
    49201 atttgtggtg ctttacgttc gacttttgca agagaaaaag cagggaaagc aatgtctccc
    49261 atttctgagt ttttaggggt ttccagtaac tttaaaatag cctcttggtc caggctatca
    49321 atgatgctag ataattcgct agcaatcaat tcttttgtat tcattaagag ctcctttttg
    49381 gacttttcta ctattttatc acaattttaa agaaagaaga aaaaattttt gaaatctcct
    49441 gtttttttgg tataatatgg ttataaatat agttataaat atgcacgcaa gaggatttta
    49501 tgagaaaaag agatcgtcat cagttaataa aaaaaatgat tactgaggag aaattaagta
    49561 cacaaaaaga aattcaagat cggttggagg cgcacaatgt ttgtgtgacg cagacaacct
    49621 tgtctcgtga tttgcgcgaa atcggcttga ccaaggtcaa gaaaaatgat atggtgtatt
    49681 atgtactagt aaatgagaca gaaaagattg atttggtgga atttttgtct catcatttag
    49741 aaggtgttgc aagagcagag tttaccttgg tgcttcatac caaattggga gaagcctctg
    49801 ttttggcaaa tattgtagat gtaaataagg atgaatggat tttaggaaca gttgctggtg
    49861 ccaatacctt attggttatt tgtcgagatc agcacgttgc caaactcatg gaagatcgtt
    49921 tgctagattt gatgaaagat aagtaatgtc ttgggagttg ctctcaagac ttatttttga
    49981 aaaggagaga cagaaaatgg cgatagaaaa gctatcaccc ggcatgcaac agtatgtgga
    50041 tattaaaaag caatatccag atgctttttt actctttcgg atgggtgatt tttatgaatt
    50101 attttatgag gatgcggtca atgctgcgca gattctggaa atttccttaa cgagtcgcaa
    50161 caagaatgcc gacaatccga tccctatggc gggtgttccc tatcattctg cccaacagta
    50221 tatcgatgtc ttgattgagc agggttataa ggtggctatc gcagagcaga tggaagatcc
    50281 taaacaagca gttggggttg ttaaacgaga ggttgttcag gtcattacgc cagggacagt
    50341 ggtcgatagc agtaagccgg acagtcagaa taattttttg gtttccatag accgcgaagg
    50401 caatcaattt ggcctagctt atatggattt ggtgacgggt gacttttatg tgacaggtct
    50461 tttggatttc acgctggttt gtggggaaat ccgtaacctc aaggctcgag aagtggtgtt
    50521 gggttatgac ttgtctgagg aagaagaaca aatcctcagc cgccagatga atctggtact
    50581 ctcttatgaa aaagaaagct ttgaagacct tcatttattg gatttgcgat tggcaacggt
    50641 ggagcaaacg gcatctagta agctgctcca gtatgttcat cggactcaga tgagggaatt
    50701 gaaccacctc aaacctgtta tccgctacga aattaaggat ttcttgcaga tggattatgc
    50761 gaccaaggct agtctggatt tggttgagaa tgctcgctca ggtaagaaac aaggcagtct
    50821 tttctggctt ttggatgaaa ccaaaacggc tatggggatg cgtctcttgc gttcttggat
    50881 tcatcgcccc ttgattgata aggaacgaat cgtccaacgt caagaagtag tgcaggtctt
    50941 tctcgaccat ttctttgagc gtagtgactt gacagacagt ctcaagggtg tttatgacat
    51001 tgagcgcttg gctagtcgtg tttcttttgg caaaaccaat ccaaaggatc tcttgcagtt
    51061 ggcgactacc ttgtctagtg tgccacggat tcgtgcgatt ttagaaggga tggagcaacc
    51121 tactctagcc tatctcatcg cacaactgga tgcaatccct gagttggaga gtttgattag
    51181 cgcagcgatt gctcctgaag ctcctcatgt gattacagat gggggaatta tccggactgg
    51241 atttgatgag actttagaca agtatcgttg cgttctcaga gaagggacta gctggattgc
    51301 tgagattgag gctaaggagc gagaaaactc tggtatcagc acgctcaaga ttgactacaa
    51361 taaaaaggat ggctactatt ttcatgtgac caattcgcaa ctaggaaatg tgccagccca
    51421 cttttttcgc aaggcgacgc tgaaaaactc agaacgcttt ggaaccgaag aattagcccg
    51481 tatcgaggga gatatgcttg aggcgcgtga gaagtcagcc aacctcgaat acgaaatatt
    51541 tatgcgcatt cgtgaagagg tcggcaagta catccagcgt ttacaaactc tagcccaagg
    51601 aattgcgacg gttgatgtct tacagagtct ggcggttgtg gctgaaaccc agcatttgat
    51661 tcgacctgag tttggtgacg attcacaaat tgatatccgg aaagggcgcc atgctgtcgt
    51721 tgaaaaggtt atgggggctc agacctatat tccaaatacg attcagatgg cagaagatac
    51781 cagtattcaa ctggttacag ggccaaacat gagtgggaag tctacctata tgcgtcagtt
    51841 agccatgacg gcggttatgg cccagctggg ttcctatgtt cctgctgaaa gcgcccattt
    51901 accgattttt gatgcgattt ttacccgtat cggagcagca gatgacttgg tttcgggtca
    51961 gtcaaccttt atggtggaga tgatggaggc caataatgcc atttcgcatg cgaccaagaa
    52021 ctctctcatt ctctttgatg aattgggacg tggaactgca acttatgacg ggatggctct
    52081 tgctcagtcc atcatcgaat atatccatga gcacatcgga gctaagaccc tctttgcgac
    52141 ccactaccat gagttgacta gtctggagtc tagtttacaa cacttggtca atgtccacgt
    52201 ggcaactttg gagcaggatg ggcaggtcac cttccttcac aagattgaac cgggaccagc
    52261 tgataaatcc tacggtatcc atgttgccaa gattgctggc ttgccagcag accttttagc
    52321 aagggcggat aagattttga ctcagctaga gaatcaagga acagagagtc ctcctcccat
    52381 gagacaaact agtgctgtca ctgaacagat ttcactcttt gatagggcag aagagcatcc
    52441 tatcctagca gaattagcta aactggatgt gtataatatg acacctatgc aggttatgaa
    52501 tgtcttagta gagttaaaac agaaactata aaaccaagac tcactagtta atctagctgt
    52561 atcaaggaga cttctttgac aattctccac ttttttgcta gaataacatc acacaaacag
    52621 aatgaaaagg agctgacgca ttgtcgctcc cttttgtcta ttttttaagg agaaagtatg
    52681 ctgattcaga aaataaaaac ctacaagtgg caggccctgg cttcgctcct gatgacaggc
    52741 ttgatggttg ctagttcact tctgcaaccg cgttatctgc aggaagtctt aggcgccctc
    52801 cttactggga aatatgaagc tatttatagt atcggggctt ggttgattgg tgtggccgta
    52861 gtcggtctag ttgctggtgg actcaatgtt gtcctcgcag cctatattgc ccaaggagtt
    52921 tcatccgacc ttcgggagga tgccttccgt aaaattcaaa ccttttctta tgctgatatt
    52981 gaacaattta atgcgggaaa tctagtcgtt cgaatgacaa atgatatcaa ccagattcag
    53041 aacgttgtca tgatgacctt ccaaattctt ttcagacttc ccctcttgtt catcggttcg
    53101 tttatcctag cggttcaaac cttaccttct ctgtggtggg tgattgttct catggtagtc
    53161 ttgatttttg gtttgactgc tgtcatgatg ggaatgatgg ggcctcgttt tgctaaattt
    53221 caaacccttc ttgagcgcat caatgccatt gccaaggaaa atttacgtgg cgttcgtgtg
    53281 gtcaagtcct ttgtccaaga aaaagagcaa tttgctaagt ttacagatgt ctcagacgag
    53341 cttcttggtc aaaaccttta cattggttat gccttttcag tagtggaacc ctttatgatg
    53401 ttggttggtt acggggcggt cttcctctct atttggctgg tcgcgggaat ggttcagtcg
    53461 gatccgtctg ttgttggttc catcgcttct tttgttaatt acctaagcca gattatcttt
    53521 accattgtta tggttggatt tttgggaaat tctgtcagcc gtgccatgat ttccatgcgt
    53581 cgtattcgag aaattcttga cgcagagcca gctatgacct tcaaggatat cccatatgaa
    53641 gagttggttg gaagccttag ctttgaaaat gtgaccttta cctatccaat ggacaaggaa
    53701 ccgatgctga aagatgtgag ctttactatt gaacctggtc aaatggttgg tgtagttgga
    53761 gcgactggtg caggaaagtc aaccttggct caattgattc cacgtctctt tgatccacag
    53821 gaaggggcta tcaaaatcgg aggtaaggat attcgagaag tgagtgaagg aaccctgcgt
    53881 aaaacagttt ccatcgttct ccaacgtgcc attcttttta gtggaacgat tgcagataac
    53941 ttgagacagg ggaagggaaa tgctactcta tttgaaatgg agcgcgcagc caatattgcc
    54001 caggctagtg aattcattca tcgtatggag aaaacctttg aaagtccagt tgaagaacgg
    54061 ggaaccaatt tctctggtgg acaaaaacaa aggatgtcga ttgcgcgtgg gattgtcagc
    54121 aatccacgta ttctgatttt tgatgattcg acctcagcct tggatgccaa atcagagcgc
    54181 ttggtgcaag aagctttgaa taaggacttg aaggggacga caaccattat tattgctcaa
    54241 aaaattagct cggttgtcca tgcagacaag atcttggttc taaatcaagg acgattgatt
    54301 ggtcaaggca cgcatgcaga cttggttgcc aacaatgccg tttaccgtga aatctatgaa
    54361 acacagaaat gaaagacaaa ctataagaaa agtcaatagt tttatctaaa ctatttctta
    54421 tttcaatttg atgatttggc gatgatttta gagcacggca aaaagctatt gaaaaagtcc
    54481 attttttcaa aggtaatcct gtgttaattt cagaaattac atcacttttt gttcgtcaaa
    54541 tggcagctct tttttaggat ataaaacagg gttcggataa gtttttttgc aaggtggatg
    54601 atggctacat tgtaatgttt tccttattct aacttagtct taagataggc cttaaaagca
    54661 ggtgaaaagc gagggcatgc tttggcagct tgtatgagta cctaccgcag atgaggggaa
    54721 ccccgtttga ccattcttcc tgctaaatca atctgacctg actgataaat agaagaatcc
    54781 agtccagcga aagcttgtaa ttgagcagga ttatcaaagg catgaatatt tcgaatctcg
    54841 gctaaaatga ccgcccctaa acgattctca atcccagtaa ccgtcgtgat gaccgagttg
    54901 aactcagcca tcaagtcatt gacacatttt ttcgccttgt caatgagcct cttgtaatgt
    54961 ttgatgattt cattacacga gataaaacgt ctatgcgtta tcaaactcat taccaattaa
    55021 aacaaaaagc tgtggttaga tcctttcgga aatcgtcaag cgattggagg aaatgaacta
    55081 atccacagcg gcttattcca agtataccac ttgggctttg gcagtagcta actgcgctaa
    55141 atataatata aggaggagta aaatgaagac agttcaattt ttttggcatt attttaaggt
    55201 ctacaagttc tcatttgtag ttgtcatcct gatgattgtt ctggcgactt ttgcccaagc
    55261 cctctttcca gtcttttctg gacaagcggt gacgcagcta gccaatttag ttcaagctta
    55321 tcaaaatggc aatccagaac ttgtatggca aagcctatca ggaatcatgg tcaatcttgg
    55381 cctgctggtt ttggttctat ttatctctag tgtaatatac atgtgtctca tgacgcacgt
    55441 gattgcagaa tcgaccaacg agatgcgcaa aggcctcttt ggtaagcttg ctcagttgac
    55501 ggtttctttc tttgaccgtc gacaagatgg cgatatcctg tctcatttta ccagtgattt
    55561 ggataatatc ctccaagcct ttaacgaaag cttgattcag gtcatgagca atattgtttt
    55621 atacattggt ctgattcttg tcatgttttc gagaaatgtg acgctggctc tcatcaccat
    55681 tgccagcacc ccattggctt tccttatgct gattttcatc gtgaaaatgg cacgcaaata
    55741 caccaacctc cagcagaaag aggtagggaa gctcaacgcc tatatggatg agagcatctc
    55801 aggccaaaaa gccgtgattg tgcaaggaat tcaagaggat atgatggcag gatttcttga
    55861 acaaaatgag cgcgtgcgca aggcaacctt taaaggaaga atgttctcag gaattctttt
    55921 ccctgtcatg aatgggatgg gcctgattaa tacagccatc gtcatctttg ctggttcggc
    55981 tgtacttttg aatgataagt ctattgaaac aagtacagcc ctaggtttga ttgttatgtt
    56041 tgcacaattt tcacagcagt actaccagcc tattatccaa gttgcagcga gttggggaag
    56101 ccttcagttg gcctttactg gagctgaacg aattcaggaa atgtttgatg cagaggagga
    56161 aatccgacct gaaaaggctc caaccttcac taagttgcaa gaaagtgttg aaatcagtca
    56221 tatcgatttt tcatacttgc ctgataaacc tattttgaaa gatgtcagca tttctgcccc
    56281 taaaggccag atgacagcag ttgttgggcc gacaggttca ggaaaaacga ctattatgaa
    56341 cctcatcaat cgcttttatg atgttgatgc tggtggtatt tattttgatg gtaaagacat
    56401 tcgtggctat gacttagata gtcttagaag caaggtggga attgtattgc aagattcggt
    56461 cttgtttagc ggaacgattc gagacaatat ccgatttggt gtgccagatg ctagtcagga
    56521 aatggttgag gtagcagcaa aagcaaccca cattcacgac tatatcgaaa gtttgcctga
    56581 taagtacgat actcttattg atgatgacca gagcatcttt tcaacagggc agaagcaatt
    56641 gatttcaatc gctcgaaccc tgatgacaga tccagaagtt ctcattctcg atgaagcaac
    56701 ttcaaacgta gatacggtga cagaaagcaa gattcagcat gccatggagg tggttgtagc
    56761 aggtagaact agtttcgtca ttgcccaccg cttgaaaacc attctcaatg cagatcagat
    56821 tattgtcctt aaagatggag aagtcattga acgtggtaac caccatgaac ttttgaagct
    56881 aggtggcttt tattcagaac tctatcacaa tcaatttgtt ttcgaataag aaagaagttg
    56941 tcctatgtgg gcagcttttt cttgtccata aaaaatgttt atcacagcct taaaaaaaac
    57001 atattagacg aaagtcattt tgagtgatat gataggacta tcgttagcat tcgaaaggag
    57061 aggcatcatg gctagaacgg ttgtaggagt tgctgcaaat ctatgtcccg tagacgcaga
    57121 aggcaaaatc attcattcat ctgtatcttg tagattcgca gagatcattc gtcaagtcgg
    57181 tggtctccct ttagtcattc ctgttggtga tgagtcagtt gtacgtgatt atgtggaaat
    57241 gattgacaaa ctcattttga caggaggcca aaatgttcat cctcagtttt atggagagaa
    57301 aaagaccgtc gaaagcgatg attacaatct ggtccgtgac gaatttgaat tggcactctt
    57361 gaaggaagcg cttcgtcaga ataaaccaat tatggcaatc tgtcgcggtg tccaacttgt
    57421 caatgttgcc tttggtggaa ccctcaatca agaaatcgaa ggtcactggc aaggactacc
    57481 tttcgggaca tctcactcta ttgagacagt agaaggaagc gtggtggcta agctatttgg
    57541 gaaagaaagt caggtcaact cagtccatcg tcaaagcatt aaagatttgg cacctaattt
    57601 ccgtgtaact gctattgatt caagagacca aactattgaa gcgattgagt ctatcgacga
    57661 gcaccgcatt atcggtttac agtggcatcc agagtttctg gttaatgaag aagatggcaa
    57721 tttagaatta tttgagtatt tattgaatga actgtaacga ttggaacatc tggtcgttct
    57781 ttcttttatt ttttcaaaat ttttagaatg tggtatttta cgcaaacgtt tgaattctga
    57841 taaaaattgg agaaattcga caaaaaaact tgaaaaaaac gaaggtaagc gttatgatag
    57901 aaaagaagaa atattggagg aagaacatgt cacatattaa atttgattat tcaaaagttt
    57961 tagacaaatt tgttgctcca catgaagtgg aatacatgca atcacaagta acagcagcag
    58021 atgaattgat ccgtaaagga actggtgctg gtagcgactt cttgggatgg ttggaccttc
    58081 ctgaaaaata cgaccgcgaa gaatttgacc gcatcttgaa agctgctgag caaatcaagt
    58141 cagacagcga tgttttggtt gtgatcggta ttggtggatc ttaccttggt gcaaaagcag
    58201 caatcgactt cttgaatcac cattttgcta acttgcaaac aaaagaagaa cgcaaagctc
    58261 cacaaatcct ttacgcagga aactcaatct catctactta ccttgctgac ttggtagagt
    58321 atgttgcaga caaagacttc tcagtaaacg taatttctaa atcaggtaca acaactgaac
    58381 cagcgattgc tttccgtgtc tttaaagaac tcttggttaa gaaatacggt caagaagaag
    58441 ctaacaaacg tatctatgca acaactgacc gccaaaaggg tgctgttaag gttgaagcag
    58501 acgctaacgg ttgggaaaca tttgttgttc cagatgatat cggtggacgc ttctcagtat
    58561 tgacagccgt tggtttgctt ccaatcgcag catcaggagc tgacatcaaa gctcttatgg
    58621 aaggtgcgaa tgcagctcgc aaagactaca cttcagacaa aatctctgaa aacgaagctt
    58681 accaatacgc agccgttcgt aacatccttt atcgtaaagg ctatgcaact gagatcttgg
    58741 taaactatga gccatcactt caatacttct cagaatggtg gaaacaattg gctggtgaat
    58801 cagaaggaaa agaccaaaaa ggtatctacc caacttcagc caacttctca actgacttgc
    58861 actcacttgg tcaatttatc caagaaggaa ctcgtatcat gtttgaaaca gttgtccgtg
    58921 ttgacaaacc tcgtaaaaac gtgcttattc ctactttgga agaagacctt gacggacttg
    58981 gttaccttca aggaaaagac gttgactttg taaacaaaaa agcaactgac ggtgttcttc
    59041 ttgcccacac agatggtgat gtaccaaaca tgtatgtgac tcttccagag caagacgctt
    59101 tcactcttgg ttacactatc tacttcttcg aattggcaat tgccctttca ggttacttga
    59161 atgctatcaa cccatttgac caaccaggtg ttgaagctta taaacgtaac atgtttgccc
    59221 ttcttggaaa accaggattt gaagaattga gcaaagaact taacgcacgt ctataataga
    59281 agaaaagagt ggtttgccca ctctttttac tctctttatc catagaaatt ggactcagcc
    59341 aagacttgtg atataatata gaaagcaaaa aggcagacgc ctagataata ggagaaacta
    59401 tgtcaaaaga tatccgcgta cgttacgcac caagtccaac aggactacta cacatcggaa
    59461 atgctcgtac agcattgttt aattacttgt atgcgcgcca tcatggtgga acatttctca
    59521 tccgtatcga agatactgac cgtaaacgcc atgtcgagga tggtgaacgt tcacaacttg
    59581 aaaaccttcg ctggttaggc atggattggg atgaaagtcc agaatcacat gagaattatc
    59641 gccagtctga gcgtttggac ttgtatcaaa aatacattga ccaactatta gctgaaggaa
    59701 aagcctataa atcttacgtt acagaagaag agttggcagc tgaacgcgaa cgccaagaag
    59761 tagctggcga aacaccacgc tacatcaatg aataccttgg tatgagtgaa gaagaaaaag
    59821 cagcttacat cgcagaacgt gaagcagcag ggatcatccc aactgttcgt ttggctgtca
    59881 atgagtcagg tatctacaag tggcatgata tggtcaaagg cgatatcgaa tttgaaggtg
    59941 gcaatatcgg tggtgactgg attatccaaa agaaagacgg ttacccaact tacaactttg
    60001 ccgttgccat cgatgaccat gatatgcaaa tctctcatgt tatccgtgga gacgaccata
    60061 ttgctaacac accaaaacag ctcatggttt atgaagcact tggttgggaa gctccagagt
    60121 ttggtcacat gaccttgatt atcaactctg agactggtaa gaagttatct aaacgtgata
    60181 ctaatacact tcaatttatc gaagattacc gaaagaaagg ttaccttcca gaagcagtct
    60241 ttaactttat tgctcttctt ggttggaacc caggtggtga agatgagatt ttctctcgtg
    60301 aagaactcat taaacttttt gatgaaaacc gcctcagcaa gtcaccagca gcctttgatc
    60361 agaaaaaact agactggatg agcaatgatt atatcaagaa tgcagacctt gaaaccatct
    60421 ttgaaatggc aaaaccattc ttagaggaag caggccgttt gactgacaag gctgaaaaat
    60481 tagttgagct ctataaacca caaatgaaat cagtagatga gattatccca ttgacagatc
    60541 ttttcttctc agatttccca gaattgacag aagcagagcg cgaagtcatg acggatgaaa
    60601 cagttccaac agttcttgaa gcattcaaag caaaacttga agcgatgaca gatgataaat
    60661 ttgtgacaga aaatatcttc ccacaaatta aagcagttca aaaagaaaca ggtattaaag
    60721 ggaaaaatct tttcatgcct attcgtatcg cagtttcagg cgaaatgcat gggccagaat
    60781 taccagatac aattttcttg cttggacgtg aaaaatcaat tcagcatatc gaaaacatgc
    60841 taaaagaaat ctctaaataa agaggataca ataatggaca tctgggaaaa gatgtacgaa
    60901 gaagcacaga aactatacaa tccacatgaa gtatcagact ttgtttatgc caatcatgtt
    60961 gtagctgcag tagaagcaga agatggacaa atatttacag gattctgtat ggagggaacc
    61021 tgtggtgttt tccatctctg cgcagaacgg gcggccctct tcaatatgta ccaattttca
    61081 ggacaaacta aggttaagaa agtattagcc tttcgagaca aaccacctta tggtggaagt
    61141 tcagccatgc cttgcggtgc ttgtagagaa ttccttttag agcttaatgc tgaaaataaa
    61201 gatgcagaat tcatgatgga ctataatata agaaaaacag ttaaagtcgc agaactaatc
    61261 ccttattggt ggggagaaga acgtgcttct aagtttaatg agcgatagaa gagtcagtat
    61321 aattctggct ttttttacta taagttgaaa aaataaaaaa aaattagaaa aagtagttga
    61381 caaagtttga aaaggctgta taatagtaag agttgaaaat aacaactcag gtccgttggt
    61441 caaggggtta agacaccgcc ttttcacggc ggtaacacgg gttcgaatcc cgtacggact
    61501 atggtatgtt gcggttggaa cacttgatga aaaaaagttt aaaaaa
//
LOCUS       DAVKJD010000005        54306 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKJD010000005 DAVKJD010000000
VERSION     DAVKJD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54306
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.005"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(120..1316)
                     /gene="tuf"
                     /locus_tag="VW157_000309"
     CDS             complement(120..1316)
                     /gene="tuf"
                     /locus_tag="VW157_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9551669.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2237..3106)
                     /locus_tag="VW157_000310"
     CDS             complement(2237..3106)
                     /locus_tag="VW157_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9551670.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3330..4007)
                     /locus_tag="VW157_000311"
     CDS             complement(3330..4007)
                     /locus_tag="VW157_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9551671.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKI
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPIPEP
                     TPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGFF
                     KKKEDEK"
     gene            complement(3965..5071)
                     /locus_tag="VW157_000312"
     CDS             complement(3965..5071)
                     /locus_tag="VW157_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9551672.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5182..5436)
                     /locus_tag="VW157_000313"
     CDS             complement(5182..5436)
                     /locus_tag="VW157_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551673.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(5776..6222)
                     /locus_tag="VW157_000314"
     CDS             complement(5776..6222)
                     /locus_tag="VW157_000314"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9551674.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDLLREVPTIFECLECFFKTR"
     gene            complement(6295..6620)
                     /locus_tag="VW157_000315"
                     /pseudo
     CDS             complement(6295..6620)
                     /locus_tag="VW157_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284991.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6854..8572
                     /locus_tag="VW157_000316"
     CDS             6854..8572
                     /locus_tag="VW157_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9551675.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8683..9030
                     /locus_tag="VW157_000317"
     CDS             8683..9030
                     /locus_tag="VW157_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9551676.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9309..10145)
                     /locus_tag="VW157_000318"
     CDS             complement(9309..10145)
                     /locus_tag="VW157_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9551677.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10158..10787)
                     /locus_tag="VW157_000319"
     CDS             complement(10158..10787)
                     /locus_tag="VW157_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9551678.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLDNLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(10797..11438)
                     /locus_tag="VW157_000320"
     CDS             complement(10797..11438)
                     /locus_tag="VW157_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9551679.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11584..12813)
                     /locus_tag="VW157_000321"
     CDS             complement(11584..12813)
                     /locus_tag="VW157_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9551680.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEYYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(12803..13969)
                     /locus_tag="VW157_000322"
     CDS             complement(12803..13969)
                     /locus_tag="VW157_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9551681.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVAISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14113..15126
                     /locus_tag="VW157_000323"
     CDS             14113..15126
                     /locus_tag="VW157_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9551682.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15171..15590)
                     /locus_tag="VW157_000324"
     CDS             complement(15171..15590)
                     /locus_tag="VW157_000324"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9551683.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15601..17007)
                     /gene="atpD"
                     /locus_tag="VW157_000325"
     CDS             complement(15601..17007)
                     /gene="atpD"
                     /locus_tag="VW157_000325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9551684.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17093..17971)
                     /locus_tag="VW157_000326"
     CDS             complement(17093..17971)
                     /locus_tag="VW157_000326"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9551685.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(17987..19492)
                     /locus_tag="VW157_000327"
     CDS             complement(17987..19492)
                     /locus_tag="VW157_000327"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9551686.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19507..20043)
                     /locus_tag="VW157_000328"
     CDS             complement(19507..20043)
                     /locus_tag="VW157_000328"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9551687.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(20043..20537)
                     /gene="atpF"
                     /locus_tag="VW157_000329"
     CDS             complement(20043..20537)
                     /gene="atpF"
                     /locus_tag="VW157_000329"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9551688.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20551..21267)
                     /gene="atpB"
                     /locus_tag="VW157_000330"
     CDS             complement(20551..21267)
                     /gene="atpB"
                     /locus_tag="VW157_000330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9551689.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21302..21502)
                     /locus_tag="VW157_000331"
     CDS             complement(21302..21502)
                     /locus_tag="VW157_000331"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9551690.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(21577..22584)
                     /locus_tag="VW157_000332"
                     /pseudo
     CDS             complement(21577..22584)
                     /locus_tag="VW157_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(23029..23427)
                     /locus_tag="VW157_000333"
     CDS             complement(23029..23427)
                     /locus_tag="VW157_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9551691.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(23755..24237)
                     /gene="greA"
                     /locus_tag="VW157_000334"
     CDS             complement(23755..24237)
                     /gene="greA"
                     /locus_tag="VW157_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9551692.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(24308..25963)
                     /gene="mltG"
                     /locus_tag="VW157_000335"
     CDS             complement(24308..25963)
                     /gene="mltG"
                     /locus_tag="VW157_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9551693.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(26044..26538)
                     /locus_tag="VW157_000336"
     CDS             complement(26044..26538)
                     /locus_tag="VW157_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9551694.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(26535..26999)
                     /locus_tag="VW157_000337"
     CDS             complement(26535..26999)
                     /locus_tag="VW157_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9551695.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDYQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(27009..28343)
                     /locus_tag="VW157_000338"
     CDS             complement(27009..28343)
                     /locus_tag="VW157_000338"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9551696.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(28354..28971)
                     /locus_tag="VW157_000339"
     CDS             complement(28354..28971)
                     /locus_tag="VW157_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9551697.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(29019..32117)
                     /locus_tag="VW157_000340"
     CDS             complement(29019..32117)
                     /locus_tag="VW157_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9551698.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYIIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFAVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTEAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(32210..33376)
                     /locus_tag="VW157_000341"
     CDS             complement(32210..33376)
                     /locus_tag="VW157_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9551699.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IHQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNVAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            complement(33384..34478)
                     /locus_tag="VW157_000342"
     CDS             complement(33384..34478)
                     /locus_tag="VW157_000342"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9551700.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGSKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            complement(34636..36078)
                     /locus_tag="VW157_000343"
     CDS             complement(34636..36078)
                     /locus_tag="VW157_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9551701.1"
                     /translation="MRKKVIEGLIYAEKSFFDKSQSGELTSAIVNDTSVIREFLITTF
                     PNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMMFIILPLSNISEKYSRRLQEEIG
                     FLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRITSFETPFALLFI
                     FATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVYSRSKGSSVA
                     LENLLAVPKEKFEGGKSVSGRGLNFNHVYFGYDENRPVLKDIICSIFKGQKIAFVGPS
                     GSGKSTIVRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDN
                     LCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGQSQRLQIARA
                     YLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLE
                     EGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            complement(36084..36317)
                     /locus_tag="VW157_000344"
     CDS             complement(36084..36317)
                     /locus_tag="VW157_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEU9551702.1"
                     /translation="MKLKLLRVDTKVIMGSFFLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKR"
     gene            complement(36489..38447)
                     /locus_tag="VW157_000345"
     CDS             complement(36489..38447)
                     /locus_tag="VW157_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9551703.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(38650..40272)
                     /locus_tag="VW157_000346"
     CDS             complement(38650..40272)
                     /locus_tag="VW157_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9551704.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            40378..41823
                     /locus_tag="VW157_000347"
     CDS             40378..41823
                     /locus_tag="VW157_000347"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9551705.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGVDCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(41869..42051)
                     /locus_tag="VW157_000348"
     CDS             complement(41869..42051)
                     /locus_tag="VW157_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9551706.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGLKNVFTK"
     gene            complement(42152..42760)
                     /locus_tag="VW157_000349"
     CDS             complement(42152..42760)
                     /locus_tag="VW157_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU9551707.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKASLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            complement(42785..43297)
                     /locus_tag="VW157_000350"
     CDS             complement(42785..43297)
                     /locus_tag="VW157_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9551708.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDEEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(43384..44319)
                     /locus_tag="VW157_000351"
     CDS             complement(43384..44319)
                     /locus_tag="VW157_000351"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9551709.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(44390..44689)
                     /locus_tag="VW157_000352"
     CDS             complement(44390..44689)
                     /locus_tag="VW157_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9551710.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(44679..45428)
                     /locus_tag="VW157_000353"
     CDS             complement(44679..45428)
                     /locus_tag="VW157_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9551711.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(45482..45841)
                     /locus_tag="VW157_000354"
     CDS             complement(45482..45841)
                     /locus_tag="VW157_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9551712.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(45843..47243)
                     /locus_tag="VW157_000355"
     CDS             complement(45843..47243)
                     /locus_tag="VW157_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9551713.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(47538..47777)
                     /locus_tag="VW157_000356"
     CDS             complement(47538..47777)
                     /locus_tag="VW157_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9551714.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(47809..48279)
                     /gene="ssbA"
                     /locus_tag="VW157_000357"
     CDS             complement(47809..48279)
                     /gene="ssbA"
                     /locus_tag="VW157_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9551715.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVLDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(48291..48581)
                     /locus_tag="VW157_000358"
     CDS             complement(48291..48581)
                     /locus_tag="VW157_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9551716.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(48734..50080)
                     /gene="asnS"
                     /locus_tag="VW157_000359"
     CDS             complement(48734..50080)
                     /gene="asnS"
                     /locus_tag="VW157_000359"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9551717.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPVAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(50237..50461)
                     /locus_tag="VW157_000360"
     CDS             complement(50237..50461)
                     /locus_tag="VW157_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9551718.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEV"
     gene            complement(50454..51641)
                     /locus_tag="VW157_000361"
     CDS             complement(50454..51641)
                     /locus_tag="VW157_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9551719.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(51638..52126)
                     /locus_tag="VW157_000362"
     CDS             complement(51638..52126)
                     /locus_tag="VW157_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9551720.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            52447..52995
                     /locus_tag="VW157_000363"
     CDS             52447..52995
                     /locus_tag="VW157_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9551721.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            complement(53273..54001)
                     /gene="yaaA"
                     /locus_tag="VW157_000364"
     CDS             complement(53273..54001)
                     /gene="yaaA"
                     /locus_tag="VW157_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9551722.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(54080..54244)
                     /locus_tag="VW157_000365"
     CDS             complement(54080..54244)
                     /locus_tag="VW157_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9551723.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
BASE COUNT        15866 a        11967 c        10002 g        16471 t
ORIGIN      
        1 tatagtttgc tctttgattt tcattgagta ttagttcaat atctaaaaaa agaaccttgc
       61 caagcaagat tcttttagtg tctgacttaa ataattgttc ttctgggaac taaatcgaat
      121 taagcttcga tttctgtaac catacctgaa ccaacagtac gtccaccctc acggatagag
      181 aatgtagtac cttgttctac ggcgattggg tgaatcaact caacatcgat tgtcacgtta
      241 tcaccaggca ttaccatttc agtacctgct ggaagttcga ttgaacctgt aacgtcagta
      301 gtacggaagt agaattgtgg acggtagttg ttgaagaatg gagtgtgacg tccaccttct
      361 tctttagtaa ggatgtagac ttcacctttg aatttagtgt gtgggttgat tgaacctggt
      421 ttagcgataa cttgtccacg ttcgatttca tcacgttgaa caccacgaag aaggacacct
      481 acgttatctc cagcaagacc ttcgtcaagt tgtttacgga acatttcaac accagtaaca
      541 actgcttttt gagtttcttc tttgatacca acgatttcga tttcgtcgtt gactttaacg
      601 ataccacggt cgatacgtcc tgaagcaact gtaccacgtc cagtgattga gaatacgtcc
      661 tcgactggaa gaagcaatgg tttgtcagtg tcacgttctg gttctgggat atactcatca
      721 actgtgttca tcaattccat aacgatgtct tcgtatttag agtcaccttc aagagcttta
      781 agtgctgaac cttggataac tggaagatcg tcacctggga agtcgtattc tgacaatagg
      841 tcacggattt ccatttcaac caattcaagc aattcttcgt cgtcaaccaa gtcaactttg
      901 ttcatgaaga cgataaggtg tttaacacca acctgacgtg aaagaaggat gtgctcacga
      961 gtttgtggca ttggtccgtc agttgaagct actacaagga tagctccgtc catttgagca
     1021 gcaccagtga tcatgttttt aacgtagtcc gcgtgtcctg gagcgtcgat gtgagcgtag
     1081 tgacgttttt cagtttcgta ctcaacgtgc gcagtgttga tagtgatacc gcgttcgcgt
     1141 tcttctggag cagcatcgat agacgcatag tctttaggtt ggttaactga tgaaggcaag
     1201 cgacgtgcca aaacagttgt gatagctgca gttagggtag ttttaccgtg gtcaacgtgt
     1261 ccgatagtac caatgttaac gtgtggttta ctacgatcgt atttttcttt tgccatttga
     1321 gtaaaagcct ccaataaaat atattttata gatagacagt aggcaataca gtctaacttt
     1381 ccttactatt ttatcaaatt tcagctaaat tgcaagtgtt ttacaacgtt tttgtgaatt
     1441 attcttggta aaaatttaac taattaatag aaagtactaa atagacttat ccttcaagga
     1501 gttttcagca ttatccatcc actcacttgc tgaaaatgta aacagtctct ggtttatata
     1561 gtaaaatgaa acaagaatag ggcaaatcgt tcaggacagt caaatcgatt tctaacaatg
     1621 ttttagaagt agatgtgtac tattctagtt tcaatctact atacaacctc aaaactgtgt
     1681 tttgaacaac ctgcggctag cttcttagtt tgccctttga ttttcattga gtataaaatt
     1741 gcagataaaa cgataacaat cataggactc agcagagtca atgtaggtcg aagaatagcc
     1801 ccctaatcgc ttattttata gcaaaatgaa atagaaactg aacattttgt tgaggaaggt
     1861 aaatcaattc ctcacaatgt ttgagaaaga agggaggact attccaactt caacccacta
     1921 taaagcagac ttacaagata gggaacccat ctccgataat attccgataa tatatagtag
     1981 aatgaaaact gcacaatttg attggagaat tcaaaccaat ttctcacaat gttttagaaa
     2041 caagggtcta ttatttcaac tgcaacctac tatagcccat tgaagctgga atagcccact
     2101 gagacttcta aaccactgct ggagaggagt ttgtttagag tttctttcat ttccctaaaa
     2161 aatcaaaaag agagaaccag tctgatcctc tcttacttat agatggtgct cactcaaatg
     2221 ttaaaggagc tatttcttag agataaagga atttgaatac agctactgcc gcaattgctg
     2281 ctgcaatagg tgctactact ggtacccaag aataccacca ttttgaatcg cctttatgct
     2341 caccaagaac tgattttgga aggaaagcat gaaggagacg tggtcccaag tcacgggctg
     2401 ggttcaaggc aggtcctgta ggtcctccaa gtgatgttac caaagccata acgaggaatc
     2461 caagtgccaa gtgagccaca gaaagtcctg aagcagtgtg tggagccacc tgtgctttaa
     2521 tagccaagtc agaaaaatca actgtttgtc ctgcttctat tgccttttgt ttcatgaatt
     2581 gaagcacttc agcaccaaag aagtttttag tcaaaccaag agctgcaaag aacaaaacaa
     2641 atgaaccaac aaactcatta atcaaaccat tgacagttgc tgcatagcga ctttcttttg
     2701 taccatggtc aatacttgaa atagttgaga aagttcccaa gatgttattt gggttttcag
     2761 ttttcaagta gaatggacgg tatgttgcca caactaaggc ttggccaaag atagccccca
     2821 agacttgcgc gataatataa ggtaccactt gtgcccaagg gaaaagaccg ctaactgcaa
     2881 gccctagagt gaaagcaggg ttgatgtgat tcccagatac gttaccaaac atcaaggctg
     2941 ggatcataac ccccatacca taaccaacag cgatgacgat ccagccactt tggtgacctt
     3001 tcgtaccttt aagttcaacg ttggcaactg caccattccc aagaatgatc aaaatggcag
     3061 ttcccaaaaa ttcagtggca tacttcagtg cccatgtgaa atccatttgc tagattctcc
     3121 ttaaaatttt ttagatattc cttattttat caatttttac cctatttagc aactgatttc
     3181 tggaaatatt tttaaaaaaa taggattcat agtttcccag ttcaacggac ttgtaagcgt
     3241 ttttattctt cacgaaagac ctcgttctca aaaccaggaa aaaagcccac atctgtgatc
     3301 ttttccttgt tcctagattc taaaatctat tatttttcat cttctttttt cttgaagaat
     3361 cctaatccta gaccagctaa caaagtcatg ataccagcac tagccaaggt agcattagct
     3421 tctgtacctg tgtttggcaa ttcctctctc ttacctgtct cataagtcgg aacttctggg
     3481 tctggattca ctggagtttc agtttttgga gtacctggtt ctggagttgg ctctggtatt
     3541 ggagtctctt gatttggtag ttctgggttt ggtgtcggaa cacttggatg ctctggattt
     3601 ggttgtggga ttggtttttc tggagtcggt tcacctggag tacctggttc ctgaggactt
     3661 ggaatttctg gtttttctgg agttggttta tctggtgttg ataaacggtc ataccttacc
     3721 gttatttctt tatcactaga gtctgacgta acttcttgtg attcaactgt tggaatatct
     3781 ggatctttgt acttgtcaat cttaccagat acaacctcgt cccagtttcc tgttgtccat
     3841 tcaccgtagg ttacaactcc cgtgaccttg ttctcaattt ttgtacggct taaggttaca
     3901 ggttgaacaa catcttcttt tacattttgg ttcgtaactt tatcaacgta atgaatgata
     3961 cgcgttatag tcttcgtctc agtagaggtt gctgttttgg gaaccactgt ttcctcaaca
     4021 ttctcacggt agtaatagtc aactgttgca ccgtcttctg gtacgcattt gcaggagttg
     4081 ctaccaaggt gtatgttgtt ttccttgtga taactcggtc ttctttgtcc tcagttgttg
     4141 ttttcccttc aatagttttt gattctgtgg tatactcaga acctatcgct aaatcagctt
     4201 ttataacaga ctctgccaac ttctcttggc taccttcttt atagtaattg gctgttactg
     4261 tagcagtggt tggcgcttcg ctttctctat aaactaaggt cactgttcta ccttcgctta
     4321 caatattccc agttaaactt gcagaatttg tatctgcttc tttaaaagta taatattttc
     4381 cgtcagtagt agtcatgcta ctgagttttt tatctgtgac attgtagctg gtaccaatca
     4441 gttgtttttt attggtaatg taggttccgt cactttcttt ttctccaatt ccagtatcat
     4501 ttttcaatga tagcaaacgc ccttgttcat caacatagcg aactttcaca ttttctgaga
     4561 ttagttctgc caattctgag gtttttttct ttttcttgat ttcttcggtt attttccctt
     4621 tctcttcttc gggaatattt agttttggaa tgattttttc aacaacggtt cgtgatggtt
     4681 ccacagtatc ttggatgact gaaaagtcag ctagaattgg gagattatac tgaacacggt
     4741 gactttgagt gtttactcct actctttcat tattctctga aaatacttgt acggtataag
     4801 aaacaacatc ttttcctaat agaacatccc cagtagagaa atagccgcct tttcctagtt
     4861 tgctatctcc agagcccact tctttcctaa tcttatcaga tagtttttta ccagtcagta
     4921 cattcgttcg cacaatccct ttgtctaccc ctacaaagtg ggagaacttt ttgaactctt
     4981 cagaaccaga tctagcccaa ccattattaa gggcatttgc ttttgtattt gtattctctc
     5041 tcaaaagttt ggcgattaga attatattca tcggcactta gagttgctgc tatatctgac
     5101 tcttgaatac caacttcctt actaccattt ctagcggcag tatatgtgta tttatctgtt
     5161 tctacttcta gctaattatc cttaacatga taggtaattt tctcctggta taagagtctt
     5221 cagtagttta tatactctgt catcatcttt agctttagaa gcagctgagt ttagccaagt
     5281 tggggtatta ggagtatctt tatcagcctg tttcaagaca ttattcaagt catttgctag
     5341 cctattggtt atcctcactg tgaaagtttt cttccagtct gaaccacttt tattagactt
     5401 agatatgctc atgaaatcct ggtcaacatt caacacaata tcgccttgtt ttttagcatc
     5461 ttccgttgct tctgcttcaa attgatcatg gagtttttgt tgctctggtg tatagttttt
     5521 ttatttacac gatttaatgt actatttttt attttagtat taagaactcc attaatacga
     5581 tttttaaact ctgcttcaaa aaatccctta atttgttggt gcgcaagttc aacattcaaa
     5641 cctttcttat acaagtcttc attctgaacg atttcggcta cacggtgaat ggtttgattg
     5701 tataccgtct tctcttaacc agcagtatcc ttagttttaa tgactactcg ataggttcct
     5761 ttagaaccgt tatagtcatc tagttttaaa aaagcattcc aaacattcaa aaatagttgg
     5821 aacttccctt aataaatccg tcactttctt tttcaagata gcccaagctt gctcaatagg
     5881 attcaaatcc ggtgaataag gtggtagagg taagaaaaag tgtttatctt ggatgcagag
     5941 ttcatccaaa atgttcttgg gatgaaaacc agcattgccc atgacaataa catgaggtgt
     6001 cttcaaagca ggtaggagtt gcgttttgaa ccactccaca aagaaatcgc ttgtcatgct
     6061 tttcttgtaa atcatgggag ctataaactc tccgtctact tgtcctgcaa caattgaagt
     6121 tcgctcaaaa cgacgtccgc taatcttttc atagactttc tcccctctag gagcccctgc
     6181 ataaggacga tagagatagc ggtcgattcc tgtttcatca atataaacag gtgctaggtg
     6241 ctttaaacta ttaaaattct taagaaataa ggctactttt ctgggtcttg ttccttaaag
     6301 ctcctcatct ttttttaaag tgaccttaat ctgctttaaa gctgcccata ctgaaggaac
     6361 agcacaatca aaatgtgccg caatttcccg taaaaaagca tctggatgag cctctacaaa
     6421 ggctttcaat tcctctaaag gaatctttcg gtttttgacg actcgctttt tcctctctat
     6481 gtgttcttgt tcacgtcttt cttttcccac gtgaagagag ttctgacgcc aacaccaaaa
     6541 aacttggctg cctcgacatg gctgtgcccc tctttgatgt aatctaatgc tcgctgttta
     6601 aaatcagtac tatatgccat agctttatta taatatgctt gtttaaaact aagtaactat
     6661 atcaatgttt tgttatgaga agacttactt tttttactga aattgaattt ttcctagcct
     6721 caacatcata taaccccaaa attcaatctg acctggtttg aagagatttt cgaagcagat
     6781 aaggttttac taaaactaaa aaaaatgata taattaaact gattaaatgt tttaaaaata
     6841 aaggagaatt tgaatgtctt accaagaaaa ttaccagaaa tgggttgatt ttgtggagct
     6901 tcctgactac cttcgtcaag atttggaaaa tatggacgaa aaaactaagg aagatgcctt
     6961 ctatacaaat cttgaatttg gtactgcagg tatgcgtggc ttggttggtg ctggtacaaa
     7021 ccgtatcaac atctacgttg ttcgccaagc tactgaagga ttggctcgtt tgattgagtc
     7081 aaaaggtgga aacgagaaag aacgcggtgt agcaattgcc tacgatagcc gtcacttctc
     7141 acctgagttt gcctttgaat ctgcggcagt tcttgctaaa cacggcatca aatcttacgt
     7201 atttgaaagc cttcgtccaa ctccagaact atcatttgca gttcgtcatc tcaactgttt
     7261 cgcaggtatc atggtcacag ccagccacaa ccctgctcca tttaacggtt acaaggttta
     7321 cggtgaagac ggtggacaaa tgcctccaca cgatgcggac gctttgacta cttatatccg
     7381 tgcaatcgaa aacccatttg cagttgaagt tgctgatgtg gaaactgaaa aagcttctgg
     7441 cttgattgaa gttatcggcg aagctgttga catagaatac cttaaagagg ttaaggacat
     7501 aaacatcaac ccagccttga ttgaagaatt tggtaaagac atgaagattg tctacacacc
     7561 acttcatggt actggtgaaa tgttggctcg tcgtgctctt gcccaagcag gatttgactc
     7621 tgttcaagtt gttgaagcgc aagcaactgc tgacccagac ttttcaactg taacatctcc
     7681 aaacccagaa agccaagcag cctttgccct tgctgaagaa cttggtcgtc aagttggtgc
     7741 agatgttctt gtcgcaactg acccagacgc tgaccgtgtt ggtgttgaag ttcttcaaaa
     7801 agatggtagc tacctcaacc tttcaggtaa ccaaatcggt gctatcatgg ctaaatacat
     7861 cttggaagct cacaaaaacg ctggaactct tcctgaaaat gccgctctct gcaaatctat
     7921 cgtttcaact gacttggtaa cgaagattgc tgaaagctac ggcgcaacta tgttcaacgt
     7981 cttgacaggt ttcaaattta tcgctgaaaa aatccaagaa ttcgaagaaa aacacaacca
     8041 cacttacatg atgggatttg aagaaagctt cggttacttg attaaaccat ttgtacgtga
     8101 taaagatgcc atccaagccg ttcttgtcgt tgctgaactt gctgcctact accgttctcg
     8161 tggtttgaca cttgctgacg gtatcgaaga aatctataaa gaatatggct actacgcaga
     8221 aaaaacaatc tctgttactc tttcaggtgt cgatggtgct gaacaaatca aagcgattat
     8281 ggctaaattc cgcaacaatg ctccaacaga atggaacgca acagctatca ctgtcgtaga
     8341 agacttcaag gcacaaactg ctactgttgc tgacggtact gttacaaact tgacaactcc
     8401 tccaagtgat gtgttgaaat acacacttgc tgacggttca tggattgccg ttcgcccttc
     8461 aggtacagaa ccaaaaatca agttctacat tgcagttgta ggggaaacca acgaagaatc
     8521 acaagctaag attgctaaca tcgaagcaga aatcaatgca tttgtaaaat aaagacctat
     8581 aaaagatgag accaaccaac ctcatctttt ttcgtatcaa atctaagcaa ttttagaaac
     8641 tttatggcat actagactta tcaatgaaag cgaggtaatc tcatggaaca atttttagat
     8701 aacatcaaag accttgaagt cactacagtt gtgcgtgcgc aagaagctct tgataaaaaa
     8761 gaaactgcaa ccttctttat cggtcgcaaa acttgccctt actgccgtaa atttgcaggt
     8821 acattgtcag gtgtcgtagc tgaaaccaaa gctcacattt acttcatcaa tagtgaagaa
     8881 ccaagccaac tcaatgattt gcaagcattc cgctcacgct atggaatccc aactgtacca
     8941 ggttttgttc atattacaga tggacaaatc aacgtccgtt gcgactcttc aatgtcagca
     9001 caagaaatca aagatttcgc aggattgtaa aaaatcagct aacaagtctt aaaatcacaa
     9061 aaacgcataa tgtaaggtgc aaaaaccttg atattatgcg ttttattgtt cctaaaacag
     9121 ttacatcagt atttcattac agaacatttt aattttggaa gtcaaatcaa tttttaacaa
     9181 tgttttagaa atcacagtgg gctattccat attcaattat ccatattcaa tcgctattat
     9241 cgttttccta gattacaaaa aaaactacgt tttgctaatc aaaacgcagt ttcagccact
     9301 attttatctt actgtccttc ttttacttct ttggttgcta catcttctcc aaaccatttt
     9361 tggctgattt cttggaactt gccgtccttg taaagactag aaaaagcttc atttatcttc
     9421 ttaaccaagt ttgtatcttc cttacgggct ccaaccgcaa aagcttctgt ttctagtcca
     9481 actgtaaaga cattataatc gtttaaaaca ccttctgctt ctaaataata gtttgcatag
     9541 acacggtcaa tcaatagacc atcaattcga tcgtttttca aatcaatcaa ggcttcatta
     9601 aaggtttggt attgattcgc ttccttatta gcgacaatat tcttcaaaat ttctggattt
     9661 gcttcaaagt ccgcataacc agatgaacca gcttgagctc ctaatgtctt tccagtcata
     9721 tcctttgcag tcgtgatacc agatgatttc ttcgtaacca atacctgctc attcttcata
     9781 tatgagttac tgaaagccac cttttcacgg cgttcgtctg tagcggaata gccattccaa
     9841 atcagatcaa tcgttccttt tgtcaattca gcttctttca aatcccaatc aatcggttgc
     9901 caatttaccg tgattccgta tttttcaaaa acagctgtag ctaaatcaat atcaaatcct
     9961 gcataagaac catctttctg agcaaatccc attggaacaa aagtactatc aaatccaata
    10021 gtaatagact tgttagactg gtactttgac caattatctc cactagtttc gctagaattt
    10081 ttcccacaag ctactaagaa caaagcagtc atcagactga ctaatacaag catccatttt
    10141 ttcatccatt tttcctccta tttaggttct actttcaata atacatctgc gatattttca
    10201 gcaaactgca aatcatgggt aaccacaatc tgggtcatcc caagttccct attttgcaag
    10261 attagcttct ccacttccaa acgtaattct ggatccaggg cagaagttgg ttcatcgtag
    10321 ccaatgattt ctgggtcaat catcatagca cgcgccaaag ccacccgctg cttttgccca
    10381 ccagatagtg agaaaggata ggcctctgcg tgtcctccta gtcctaactg ttccaagagt
    10441 ccactcgcct tcttctcagc ctcttcctgc ttcattccca tggtcttcac aggcgataag
    10501 gtcaaattgt ccagaactga taaatgaggg aacagttgaa aatcttggaa gacaaatccc
    10561 agtagattgc gcttctgcaa ttcatccagc tctaaaggtt gtccattata aaagatttgc
    10621 cctgaatcaa tggtttcaag acctgcaagc atacgtaaaa gagttgtctt acctccacca
    10681 gaaggtccaa cgatagccag gatttgcttt tcaggaatac ttagactgaa attagacagg
    10741 atttgtttgt ctccaaagac tttattgata tttcgtaatt ctaacatggc agcctcctat
    10801 ctataataac tatacttctt ctcaactttt ttggaaataa ttgtcacaat cccaatcaaa
    10861 atcaaataaa tggctcctgc caagaacata ggaactagac tagcatcgcg gttagcagct
    10921 gttcgactag ccaagataag gtctgaaatt ccgagagcat agaccaaaga agtatccttg
    10981 accaaactca taacttcatt aaagacacta ggaagaacga tcttggtcac ttggggcaag
    11041 ataatatagc gcactctgtc aaaagggcta aacttcaaga ccttggcggc ctcatactgt
    11101 cctcttggaa tagtgtcaat tcccccacgg aaaatttctg caaagtaagc tgcatagttg
    11161 agaacaaagg caataatagc tgcaggaagg cggtctaaac gaatcccaat acttgggagc
    11221 acataataga taaaaatcag ttgcaagagt aagggggttc ctcgcataac ccagatgtaa
    11281 atgttaatca gataatggag gggcttccaa tggacttgca aggcaaaggc aatcaaaacg
    11341 cccaacggaa tcgaaaatag caagaccagt gcaaagacct gtacagtcgt gctcgcacca
    11401 ttcagtaaac tcggtaatat ctcaaataaa taagacatac tgcacctcct aaaagtaatt
    11461 gttcctatta taacatgaat aaacaagatt accaagtatt tttgtaaaaa gattctacca
    11521 tatacaaata aaaaccgtag ctatcaaaag agataaacta cagttctata tgtttaaaca
    11581 ttcttacaat ctctcaaaca gttcttgcat ttgcacatca tctggaacca gttttaagta
    11641 agtgtgagca tggacttttg cttcttcaaa atgtcccaat tcacgcaaga gatagatata
    11701 gtattccaga aattctggat tgtccttcaa atctcctgtc aactcttgat aatgctcata
    11761 agcagtatcc aaatcgtcca tttcttgata agaacgagca atcatccact tggtcaaaag
    11821 attttctggc tcctcactct gcaagtctag aatatcctca taacgctcct gctccagata
    11881 aatagtggct aaacgaagca agatttcttc tgtatcctca gcgtcttctt ttgcagtaag
    11941 gagataattt tctgcaccac tagcatcatg caattcataa gaaaattgtg aagcagctag
    12001 caagaggcga gtttcaaagg gatttttctc taatccttgc ttagcgatac gcagggcttc
    12061 ttgaacttga tgttccttat gtaaagcctg actgtaccca tactcatagc cttcaaagtc
    12121 aggagaaatg gtatcaagct gcttaaagta gagggtggct ttttgatatt cttcttgatc
    12181 aaaataaaga ctggccaact caaaagctgt taagtcatcg tattctaact ccagggcttt
    12241 ttctaaaaac tcagtagccg tttcaaattt ccctaactga gcataggcaa agccaattcg
    12301 ttgataggtg gaaatgcccg tttgctcata aatcgagcga ttatctaact gggcataggc
    12361 ttgaatagcc gcttggtaat tttccaactc actatccaac tctgccaaac ccaatatcaa
    12421 gagagaatcc tctgagtagg tcaaggcctc caataatttc tcacgtgcca catctgtcaa
    12481 accttccagc tggtaaaggt ctgccttcag agccaaagac gagacatacc agtcactgtc
    12541 agcttggatt tcctcaagat aggtaaaggc ttcttctatt tgaccatcct cgctagcaat
    12601 tgcagctaga ttaagatgaa cctctggaaa atcctctaca attttcaggt aaatttcctt
    12661 ggcctgagga tagaaaccaa tcccttcaag ataagttgcc aactcataca gaagatcact
    12721 tgaatcattt tctaaagctt tggcgaaata atgctcagcc ttagttaaat cttgctcctc
    12781 caaagcctgt aacatctgtt gactattgtt cactcttgac ttcctcaccc tctaattcat
    12841 atagaccact gactacctta taccattcga aaatggctga aatggcaacc ttagcagagg
    12901 cataaaccgg aataccaagt aaaactcccc agataccaaa catagatcct gaagttaaca
    12961 aaacaaagag aacattaata ggatggatgt ttaattgact tcccaaaatc aatggagaga
    13021 caaaacggcc ttcaatagtt tgttctacga taaagacaat cactactttc aaaagcatga
    13081 ctggaccagc aatcaaaccc aatactagag caggaagcat ggctagaaag ctaccaagat
    13141 aagggaccag atttaaaata ccagcagtaa cccccagcgt aaccgcatag cgtagaccaa
    13201 taatcttgaa gaagatgata aacattactg ctacaataat agccactgtc acttgccctc
    13261 gaacatagtt ggacaactgt tgattcacat ctgataaaac ttgtccaaca ggttccttca
    13321 attttcttgg aatgaattgg gtcaaatagt tacgcaagcc tttcccatca cgcaagagat
    13381 aaaagagcat gaaaggaacg ataatcaagg caacaatcac ttgagaagcc ccgctaataa
    13441 aggcactcac ccagttgact gcctgagatg aaaccttact tgccaaaact gtagcctggc
    13501 tagaaaaatt ggtcaaaact tgctctaatt gaggtctgaa atcatctggc aggtgctgag
    13561 ctaccaatcc attaacaatc ctatctatat cttctaagta aacaggaacg tttcttgcaa
    13621 aggtcaaaac ctgacgttgc agatttggaa tggcgactgc caagccccaa atgataaaga
    13681 gagcgatgat aacaaagaca atagtgatag ctataacacg attaacctta tgcttctcca
    13741 tccaatcaac aataggattc aacaaataat ataacaaacc agacaaaatg actggcaaca
    13801 tcacaactgc taaaaagtct aaaacaggtg aaaatagaaa actaatctta cttaaaataa
    13861 aaagattcag tcccaataat aaggttacta aaaataccgt aattgccttg ttatctaaaa
    13921 accacttgaa aaaccaagat aggctaaaat gtttctcttt ttgctccata aatacttcct
    13981 ttctattgtt ctctcattat accatttttg aaacaaaata actctttttt aaagtgctta
    14041 catctaattg gatgtcttta atttgcttat ttttgtgata taatagtctt atctttacac
    14101 agaggtatag taatgaaaga aactgtttat tttggaactt atacacgtcg tacttctcaa
    14161 gggatttaca aggcagactt tgatacagaa actggtcagc tttcaaatct agaacttttt
    14221 gcagctgagc caagtccaac ctaccttgcc tttgaccagc accaacattt atacactgtt
    14281 ggtagccaag acgataaggg gggaattgca gcctatcaaa ctgacgggac tgtgttaaat
    14341 catgttgttg aagaaggagc tccccactgt tatgttgctg tcgatgaaaa gcgtgatttg
    14401 gtttacgcag ccaactatca caagggacaa gtccttgttt ataaacgcca ggaagatggt
    14461 agtcttctac ttagtgatat ggatcaacac agtggccaag gtccacatga aaatcaagct
    14521 tccccccatg ttcactatac agatttaaca cctgaccact atctagtgac ctgcgacttg
    14581 ggaactgacc aagtcatcac ctatgacctc gatcaagaag gaaaattatc taagctctat
    14641 acctatcaca gcaagccagg agcaggctca cgccatatca ttttccataa ccactataaa
    14701 atcgcttatc tcatttgtga actcaatagt actatcgaag ttttaatcta cgatggcgtt
    14761 ggcgaatttg tacgtatgca ggttatttca actttaccag aagcttacga aggctttaat
    14821 ggaaccgctg ctattcatct ctctaaagac ggtaaatacc tctacgcttc taaccgtggc
    14881 catgattcta tcgcagtata taccatcctt gcggacggta gcttagagtt attagaaatc
    14941 gttccaacgc atggtcagac tccacgtgat tttgatttga cccccgacca aaaatttctc
    15001 attgttgtcc atcaagactc tgacaatgca actgtcttta aacgtaattg tgacaatggt
    15061 cgtctagcag aactctccaa cgacttccat gttcccgaag cagtctgcat ccgttttgct
    15121 ccttaaaaca ccataaaaaa tgaacttggt attttcaagt tcattttttc ttatagtcta
    15181 tttccgacat taatacggtt aatagcacgt tgcaaagcaa tcttagcacg acgttcttgg
    15241 tcaatcaaat gtttgtcttg tgcttcttca attgcacgtt ctgcacgaag tttggcacgt
    15301 tctgcacgac tgatatcgat atcacgagca cgttctgcag agtcagcgac gattgtgatc
    15361 atatcattgg caatttcaat aacgccaccg tttactgcaa tccagttcac gtgatcttta
    15421 tcatcgatac gttttacctt tacttcatca actgctaaaa ccgcaatcat attttcatgt
    15481 cgtggcaaga tccccatctc accatccaga gttcgaaccg atacatagct ggcatggtga
    15541 tcatagacga gaccatctgg tgtcacgatc tggacagtta actgagccat agatcacctc
    15601 ttaaaatccc attttttcag cttttgcaat cacatcttcg atagaaccta caccacggaa
    15661 ggcatcttct ggcaagtggt cgtatttacc atcaaggatt tccttaaagc cacgtacagt
    15721 ttcagcaact ggaacataag aacctggctg accagtaaat tgttccgcaa cgttgaagtt
    15781 ttgtgacaag aagaactgga tacgacgggc gcgagcaacc aaggtctttt cttcatcaga
    15841 aagctcatcc ataccaagga tagcaatgat atcttgcaat tcatggtaac gttgaaggac
    15901 acgttttact tcagcagcaa ctgcatagtg ctcttctcca acgatttcag gtgccaaggc
    15961 acgtgagctt gaagcaagtg ggtcaacggc tgggtagata cccaattgta ccaacttacg
    16021 ttccaagttt gttgttgaat ccaagtgagc gaaggctgtt gctggcgctg ggtcagtata
    16081 gtcatccgct ggcacataga tagcctggat agaggttaca gaacccttct tggttgatgt
    16141 gatacgttct tgcaattgac ccatttccgt agcaagtgtt ggttggtaac caacggctga
    16201 tggcatacga cccaaaaggg cagatacttc tgaaccagcc tgagtgaaac ggaagatatt
    16261 atcgataaag agaagcacgt cttggccttc cacatcacgg aagtattcag cgattgtcaa
    16321 accagtaagg gcaacacgca tacgtgctcc tggtggctca ttcatctgac caaagaccat
    16381 ggctgttttc tcgataacgc ctgattcttt catttcccag taaaggtcat tcccctcacg
    16441 agtacgttcc ccaacaccag taaatactga aataccaccg tgctcttggg caatgttgtg
    16501 aatcaattct tggattaaga cggttttacc aactccggca ccaccgaaaa gtccaacttt
    16561 accaccttta aggtaagggg caagaaggtc aataaccttg atccctgttt caaggatttc
    16621 agaagaggta gacaactcat caaaagttgg agctttttta tgaattggct gacgctctgc
    16681 gtcttctgta aaaggagctt ccaaatcaat ggtatctccc aaaacgttga agacacgtcc
    16741 caaagtttct ttacctactg gtacagagat tggacgacct gtgtccaata cttccattcc
    16801 acgagtcaac ccatctgttg attccatggc gatagtacga accataccat ctcctaactc
    16861 caaggctact tcaaggacga tttttgtttt tctttcgtca tttttgtaga cgacaagtgc
    16921 attgttaatc tcaggaagtt tttcccctgc tgcaaacaaa acgtctacaa cgggaccgat
    16981 aacctgagca attttacctg aactcatctc cttctcctat tctatataag atactgtcgg
    17041 ttcctagctc aactaggtcc taagttcatt ttcatacgag ctggactaga gcctattcta
    17101 aggcactagc acctgctacg atttctgtaa tttcttgtgt aatcgccgcc tgtctggcac
    17161 ggttatactg aattgtcaaa tcattgatga ctttcttagc attatctgtc gctgtttgca
    17221 tggctgtcat acccgcagca ttctcagctg tcttggcatc gataatggca ccgtaaatca
    17281 tactttctgc aaactgaggc aacaactgct ccagaatttc ttctcggctg gtttccaatt
    17341 caaaagtcaa gctgtactct tcatccgctt catttggatc caagtcaaca atcggaagca
    17401 tttgttccac acgcatttga ctggttagcg tattgacatg gtggttgtag caaacataaa
    17461 gctcatcaaa gagttcattt tggtacattt caacagtttt tgaaataatc ttacgaactt
    17521 gatcaaagct aggttggtct gccaagccac gtaattcata aagtggttga ataccgcgag
    17581 ccttaaagaa atcagctccc atcccaccga tacagatcat ttcaaaacct ttaccgtctg
    17641 ggtggtattc ttctttcaac tccataacag ctttcaaaat agaggaatta taacctccaa
    17701 ccaaaccgcg gtctgaagtg ataacgatat agcctgtctt cttcacagaa cggctaatca
    17761 acatcggatt agttgaagca ccagctccat taccatgaag gatatctgtc aaaagtttac
    17821 gcactttctg agcgtaaact tggaagttgc gagcagcttc ttcagaacga cctagcttag
    17881 cagccgatac catttgcatg gcattagtga tttgactcgt attttttgtt gaggcgattt
    17941 ttgttttaat atcatttaga gatactgcca tctgacacct ctattcttat tggaagctag
    18001 attgattgag aaactctgta atcgcagcat ccaagactgc ttcttctggc aagtcttttg
    18061 tatcacgaat ggtttccaaa atctctggat gttgagcatc aaagaaggta tggaactctt
    18121 cctcgaaacg aacaatatca tctactggaa cagtatccaa gaaaccatgt gtcaaagcat
    18181 aaagaatggt tacttgtttc tcaacaggta atggtttgtg aacaggttgt ttcaagacct
    18241 caacggtacg acgtccacgg ttcaacttag cctgtgttgc tgcgtccaag tcagaaccaa
    18301 acttagtaaa ggcttccaac tcacggtatg aagcaaggtc gatacgaagt gtaccagcaa
    18361 ccttcttcat ggctttgatt tgtgcagaac cacctacacg agatacagat gaacccgcat
    18421 cgatggctgg acgaatacct gcattgaaga ggccatcgcc aaggaagatt tgtccatcag
    18481 tgatagaaat cacgttggtt gcgatatagg ctgagatatc tcctgcttgt gtctcgataa
    18541 atggtagggc tgtaattgat ccaccaccaa gttcatcaga aactttagct gagcgctcaa
    18601 gcaaacggct gtggagatag aaaacatccc ctgggaaggc ttcacgacct ggaggacgac
    18661 gaagcaagag cgatagttca cgataagcta ccgcttgttt tgatagatca tcgtatacaa
    18721 tcaaaacatg cttaccttga tacataaatt cttccgccat agcaacccca gtataaggag
    18781 ctaggaagag caatggagat ggttgtgaag cagaggctgt cacaacgatt gtgtagtcca
    18841 aggcaccgta ctgacgaagt gtttctactt gcgtacgaac tgttgattct ttttgtccaa
    18901 tcgcgacgta gatacagatc atatcttgat ctttttggtt caagattgta tcaatcgcaa
    18961 tggttgtttt ccctgtctga cggtcaccga taatcaactc acgttgacca cgaccaatcg
    19021 gtacaagggc gtcaatagct ttcaaaccag tttgcaatgg ttctgaaaca gacttacgtt
    19081 gcataacacc aggagctggt gcttctactg gacgagtttt atcagtgtgg atttctccaa
    19141 gaccgtcaac tggacgacca agcggatcca caacacgacc aatcagactt tcacctacag
    19201 ggacttccat gattttccct gtacggcgga ttgtatcgcc ttcacggata tctgtaaagt
    19261 cacctaggat gataatacca acgtctgttg actccaagtt ttgagccata ccataagagc
    19321 cgttttcaaa attcaacaac tctccactca tgacattttc aaggccatga gcacgcgcga
    19381 taccgtcccc gatataggtt acaacacctg tttcagtcac atcaaaattg ggtttgaaat
    19441 tttcaatttg ttgcttaatt aaagcgctga tttcttgtgc gttaattgcc aaaagaacac
    19501 cactttctat ttcaaatttt ctttaacaac tttaagttgt tgtttaatac tcacatcaat
    19561 tgtcttgtga ttggcaaaaa tgacaaaacc accaatgaga ctttcatcga tttgttcttt
    19621 tacactcctt actttcagag acattttttt ctcaatcaaa gggagcaaac gagtcttctg
    19681 ttcatcagtt agaggatgag cagacgtaat cgtcacttca aatcgatttg tttctttttc
    19741 aagtcggttc aagcaatcta caagcacatc ataaaaaaga tttgctctgt gattgtaggc
    19801 cagaacctgg ataaagtttt gtaataaagg cgacacagaa tcttggaaaa aagcaattgt
    19861 tttttcctta tccgactcgt ctactgccac ttgttttaaa aaagaaggca gacctgtttt
    19921 ttcaacaact tgcttgattt gagtcaagtc tgaaaagata cggtcttctt ctcctttttc
    19981 aagtaccaat tggacaaaag gcatgctgta tttttcaatt acctttactg ttttcttgtc
    20041 cattaagctt ctcctagctg atcgatatac tgatcaatga gtgctttatg ggcatgactg
    20101 tcaaggtttt gtgagatgat tttaccagct aagctgatgg tcaaatctgc gacctcaccc
    20161 ttaacactct gtaaagcttc tactttattt tgagcaattt cttgattggc tttttctttt
    20221 aagtgtcctg cttctagttt agcatctgct aagatattag ccttactttg ctcagctgtt
    20281 tcctttgcat tttcaatgat tgtcttagct tctttacggc taccagccaa ttcatcttcg
    20341 cgtttttgag ccaatacttc tgctttttga cgggcttctt cagctctgtc aatatctgaa
    20401 gcaatttttt cagctctttc ttcgaaaatg cctgtaatat tagaccatgc aaatttttta
    20461 atcaagacta gcaaaagaat aaaagagcca gtgattaaaa taaaattacc aattaattca
    20521 cctactgtta cgtgcatcag ttactccttt ctattcctct tcaatattaa tcttattccc
    20581 taaatacaca gatgtcaaaa gagtaaaaac ataagcttgg atgcaggaaa taaagacaga
    20641 aaatgcagtc caagccaaat tagctccaaa ggctactgga taccaataaa tagcttggtg
    20701 ggaaagaaga agtaacaaac tcgtcatgac ctctcctgca aagatattcc caaaaatccg
    20761 caaagccaaa gataagaagt ttgtaaattc ttccaagata ttcatcggta tgacaaaaac
    20821 aggactcata aaagatttta tacttttttt aaatccacga cgacgaacgc tttctatatg
    20881 tgtcaacaaa atgacaagaa aagataaggt taagtcatac tgtaaattag cggttggcga
    20941 actccaccag ttagtcccat cgatcgtttg aagctttgtc attaagccaa ggttattggc
    21001 aatcaccatg aaaaggaata aacaaaggaa aaagagtgag taatctttca tgtagcgcga
    21061 accaatgtta ggttctgtaa atccaataac aaagtcatag acatactcaa gtacattttg
    21121 ctttcctttg ggtttcaagg tcatattgcg gcttgcccaa taaataaaga caaaaataac
    21181 tcccacaatc aacaaagtca tggctaacat agtcagattg aagataacag gaccaataga
    21241 gatgattgga ttaatacttt cttccatcta aaatcctccc ttttcttctc gttccatgta
    21301 tttatttgat aataaatgag aagacaagag ttacaaagaa agttccttca ataaaggcaa
    21361 cacctaaaaa catcaaacta cgaaactcag aaagcatatc tggttggcgt gctactgatt
    21421 taaacagtcc attcatcaat aaaccttcac cgacagatac gcccatacag gcaatacata
    21481 agcctaaaaa tgttaaattc ataacaaatt ctcctttaaa ataaaattta cttacttagt
    21541 gtagtcctaa aatttaaatt tgtcaacttt taaccgctat tggaagcgtt taataaaaat
    21601 tatttccatt ttgctaaatt tcaagttcaa gttagccatc cagaagtctt ctctgggtga
    21661 cttgtagtcc aagcattttt taggatagtt gttaatccac ttttcgatga atgcgacttc
    21721 tttgggagtc attttcttgg ttcccttagg taaccatcta cgaatgaacc tgttgtgatt
    21781 ctcattagtt cccctttccc aagaggcata ggggtgcgca taataaatgt gctcctcaga
    21841 aaatacatca gacaagcgat tgaattccgt tccattatct gccgtgatgg aaagaatctt
    21901 gtgttgtttt aagatgagtt ttagagcctg attgaccacc tcagcacttt tatttggaat
    21961 caatcggatg atctgacatc tactctttcg atccgtcaag acaagcaagc agtagttttt
    22021 cgctctcgta agtagaaccg tatcaatctc ataatgccca ttctccaagc gaaaattgat
    22081 agcttcaggc cgctgttcga tggattgacc agcaggttta aagttggtgc tggcctgttt
    22141 cttaagcgct tttccttttc tagggtaaag cagatcctgc ttgctttacc ccaattttcc
    22201 acgatgaatc caatagtaaa tggttgaaat tcccacgtta acccctttag ccatcaccat
    22261 catttcaggc aaaaattttt ggttatgata gtggagaatc ttttccttta gttccttggt
    22321 cacgcttgat ctcttgaccg agtgcttgcg attattttca taagactgtt gagcatagtc
    22381 ggcagaataa acctctttga agcgcccttt tccaagacat tttcggactg tcctacgctt
    22441 gattttagtg tggatagttt gaggagcttt tccaagtaga gaggcaattt ctctatttga
    22501 ttttccttct tttttccatc tttcgattaa gcgacggcta tcgattgtca aatgtttacc
    22561 ttttgtagta taattgtctt gcatttctgt gcctttcttg tgtttgtggt tgaacaacaa
    22621 gtataacaca gaggtgtttt cttatgccta caagagcttt cattatttcc attttctttt
    22681 ggatttcact ctattctgaa aaacttgtgt atatttactg aagctagcaa gtcttacctg
    22741 taaatttaat gaaagcaaca caaaatccga gaggggaatc tcggattaat agatagagag
    22801 tttttagttt aaataaattg tttaaaatat caacaacatc acttcttttc ttaacctgat
    22861 aagtcttgat tcctaatttt ggggctacga ttatattgtc ctcaatatcg tctagaaaga
    22921 cacaatttct aggttataac tggtatttat caatagttac tcatatacat cagtccacct
    22981 ccatacttat gtgcgagcct ctctttgtat tatacctcca tactcacctt acagattctt
    23041 ttggtaataa tatctttgcc taatgtagag atagtcttgc aaagaaaaaa cttccttgta
    23101 gccatgtttc tgataaaagt ccggtgcctg gaactggtaa gtattgacaa aggcaaaaca
    23161 acaatttcga ttcttagctt cactttctgc ctgttgcaat agttttgaac cgattccttg
    23221 ccctcgcagt tcctctttta caaacaaata ctcgatttct agccaatttc caaaagtctc
    23281 tgctatcaaa cctgccagga gattgccctt ttcatcttcg acataaagat taagtggctc
    23341 actttcagcc tcttctcttt ttgaacggtt ataaacacga atcagattcc ctatttcttg
    23401 cgatttatgt gattccttat tttccaatct aaagtatagt gaaatgaaat aaaacatgcg
    23461 caaatcgatt aaggaattta atctaatttc taacaatgtc ttagaaatca aagtgtacta
    23521 ttttaacttc aatgcactat acatctaata ctcaataaaa atcaaagagc aaactaggaa
    23581 actagccgca ggttgctcaa aacactgttt tgaggttgta gatgaaactg acgaagtcag
    23641 taaccataca tacggcaagg cgacgctgac gtggtttgaa gagattttcg aagagtagca
    23701 aaatggaaaa aggagtgagt gaagcacatc gcctccccac tcctttttct gtttttaggc
    23761 tgttttttca accttcaaga tttttacatc atagctacca acaggcgttt caatggttgc
    23821 tgtatcacct gttttcttgc caatcaaggc ctgcccaatt gggctttcat ttgaaacctt
    23881 acctgcaaag gcatccgcac cagctgaacc tacgataata taaacttctt cttcgtcctc
    23941 accaatttct tggatggtga ctgttttacc aatcgctact tcgtcctggg caactgcgtc
    24001 gctattgacg atttcagcat agcggatttt tgtttctaag ctagagattt gtccttcgac
    24061 aaaggcttgt tcatccttag ctgcttcgta ctcactgttt tctgaaaggt caccgtatga
    24121 acgggcaatc ttaatgcgtt ctaccacttc tggtcgacga accaatttca attcttctaa
    24181 ttctttttca agtttttcct tttcctcaag ggtcatagga tatgtttttt ctgccatttt
    24241 tctcaacttt cttctgataa tattttctaa agaaaattat gtgaagtatc acataatttt
    24301 agtttgttta gtttaatttg ctgttgacat gttcagcgac attgcggtcg tggtcttctt
    24361 gattgttagc atagtagacc ttgccttctg tgacatctgc tacaaagtag aggttatcgc
    24421 tcttagtttg attgatgctt gactcaatcg catccagact tggactatcg actggaccag
    24481 gcatgagacc tacattttta taaacattat aaggtgaatc aatgttggta tcaatcgcaa
    24541 catcctcagc tagactgata ttttgcccca gttttccttg ggcatacaag attgcaatat
    24601 tactttgaag tggcatatca cgattcaaac gattgtagaa tacacctgca atgagcttac
    24661 gatcttctgt cttggcacct tctttttcga ccaaggaagc aatggtcaac aactcattga
    24721 cagtcaagtt tttagatttg atagtactat agtaaggaga taggttctta tccatagcag
    24781 ctaacatctc atcaatcaag ctctcaatag ttgtgctttc cttgatagag tatgtagctg
    24841 ggaaaaggta tccttccaaa cgataacgcg caccgctgtc ttttacaggc aaactttcca
    24901 gtaaagtagg atatttcgct actgcttgac tgataaacgt ctcatcttga actttagcca
    24961 agaaagcctc cgctgtcaaa gactctttga agtcaccttg caattggccc acagtttgag
    25021 caatctgatc caaggtataa ccttctggaa ttgtcaaagt cgcaagtaca ggttcttgcg
    25081 gttcatctgt tccacctttt tgcaactctt tgagtaagtc ttctgtactc atactctttt
    25141 gcaaattgta gtaacctgct ttcaagtcgg tataattttt atacttggca taaaaactaa
    25201 aaatcagacc atgctttacc aaaccagctt tttcaagcgt cgtaccgatt tcttgaacgt
    25261 ttgaaccttc tggaattcca accgtcacat atttcttaga attagcatcg ataggtaaca
    25321 aagaatccag cacgtactgg taaccaaagt aaccacctgc tgagagcaag gcaaggaata
    25381 caaggaatga aatcacaaaa gctttcacat gtgacttctt ctctttctta ggcttaacgg
    25441 gttttgctcc ttctctacgg ctacgacgtg atgtatcgct gatgatatcc actgtttctg
    25501 tagcaggcgt aggtgtttct ggacctgctt gttctgcttt tttctctgcc gtcgtatagg
    25561 aaacagctac ccttgttggg gtttcattgt attctctttc aagtttctta ggtctaacag
    25621 gacctggacc tggtcttgat ccactttctt ccgctggaga agaaggtaca tcttgacttg
    25681 gatgacttgg aacaccagga gtttctcttt gaatctcatc tgctggagaa gctggtacac
    25741 cttgacttgg gtgagtaggc acggtaggag cttttctcat aatctcctct accgttgaca
    25801 aggaatcagc catgagttct tcagctgaag gttcatttgc aggagtgcga actactgcct
    25861 catcttcttt cagaacttca tcatagcctt ttactttttc taaatctctc agaatctgct
    25921 ctttaaagct taatttctct tcttctcttg acttttcact caaaagtttt tcctccttgt
    25981 tgataatcca taatattata tcttaaaagt ccaaaaaaag caatctatga tacttttcct
    26041 aacttatttt ttcatttccc agaccatatc ataccatgtt tcccctgcaa aggttgactg
    26101 ggactggcct tcgttgacaa atccatgttt ttcataatag gcgataagat agtcatgaca
    26161 ggttagatta atgccatctc tctcatcttc aagagcaact tctttcaagg ctgtcagcaa
    26221 tttctgaccc agtccgagtc cctgtgcctc cttggcaata gacagacagg tcacagagat
    26281 ataaccacca ggctcatgac tatagtcttt tatttcttct gtaaaagact ggtcttgcag
    26341 atggcgatgc aggccaactg gtccttcgat ataacccatg attcttcctt ctttttcagc
    26401 aaccagaaaa gaggtctgaa tttctctcaa atgtgcttca aagacagaag gaggaatggc
    26461 ttcttcgacc gaaaaatttt caaattcaag ttcaacaatc cgatccaaat cttctaatct
    26521 tgcttgtctg attttcattg ttcctccaga taaaagggat taaaccaaat catactatag
    26581 ccctggctag ttgcatagag caaagtttct tcttcatcaa caaaaccgtt catttcaaaa
    26641 taggaaagca gctcatcagg actctccaaa cgaatccctt tgtaatccag ctcaactgcc
    26701 acctctttca aggctgcaag aagaagtgtt cccaggccct gtctctgata gtcagactcg
    26761 atgactaaag aatgtacttt tagacattgc ggattgtctg actggggact tgataaaata
    26821 tagcctaaaa gttgattttc atccctagct agaagaaagg tatccgcaca cttacggata
    26881 ctttcttcta aaatatggga aagttgctgc ttttcagctg gaaaagacga ggtctgaagt
    26941 gcccctatct caggcaaatc agacttgctt gcctgaatga tcttaattgg aatttccatg
    27001 ggaacatcct attgaacatt gcttgtcaag ttagacaaga gacgctcaaa tgagtattca
    27061 taggtttgga tgtctcctgc tcccataaag acgtaaacag cattgtcatg gtctaggagc
    27121 ggagaaacat tttcaacagt aatcacttgg tgttttttgt tgattttgtt ggctaggtct
    27181 tctaccttaa cgtcaccatg atctacttca cgagccgagc cataaatttg cgctagataa
    27241 acagcatctg cttggtttaa agcatgggca aagtcgtcca acaaggcaat ggttcttgta
    27301 aaggtatgcg gttgaaagac tgctacaatt tccttgcttg ggtatttctg acgagccgca
    27361 tccaaggtcg caataatttc tgttggatga tgggcaaagt catcaataat cactgtatca
    27421 ttgacaattt tctcagtgaa acgacgttta acaccggcaa atgttttcaa gtgctcacgc
    27481 accaagttca aatcaaatcc tgctgtgtaa agaagaccaa taacggctgt cgcattcatg
    27541 atattgtgac gaccaaaggt tggaatgtgg aattgcccca agttttgtcc acggaaatga
    27601 acggtgaagg ttgaaccagt tgttgaacga agaagatcac tagctacaaa gtcattgcct
    27661 tcagcttcaa aaccataata ataaattggt gcatcagacg taatcttacg caattcagca
    27721 tcttcaccat agacaaaaag acccttggta atttgtttgg catagtcgtt aaaggcattg
    27781 aaaacatcct cgagacttgt gaaataatct ggatggtcaa agtcaatgtt ggtgataata
    27841 gagtattctg ggtggtaagg catgaagtga cgctcatatt cgtcagattc aaagacaaaa
    27901 tatttggcat tagccgaacc acgacctgtc ccatctccaa tcaagaagct ggtatctgta
    27961 atgtgagaca agacatgaga caacatacct gtcgttgaag tttttccatg tgctcctgct
    28021 actcccatgc taacaaagtc acgcataaag ctacctagaa actcatggta acgtttgtag
    28081 ctgataccat tttggtccgc ataggcaatt tcgacgttgt tatctggacg aaaggcattt
    28141 ccagcgataa tttccatatc accgtctaga ttcttttcat caaaaggaag aatggtaatt
    28201 cctgcctgct caagaccgcg ttgggtaaag tagtactttt caacatctga tccctgaacc
    28261 ttgtgcccca tctggtgcaa catcaaggcc aaggcactca tccctgatcc cttaattccg
    28321 ataaaatgat atgtctttga catgttttct cccctattct gtcattctgg tcagattcaa
    28381 ctcttgggca acccgacgtt cttgttctgt ttgtttactt tttttattgt agatttggct
    28441 cttctttaga aaatcataat tgtttttctt tggagcaggt gctgacactt cttcattctt
    28501 ggtagggata gaatgaactt cttccgccaa gatataatga gactgggtca atttttggct
    28561 atatttgaca aattcaccag gattttcctt ttggaaagga gttgtcggtt gattgccctg
    28621 tctaactaga ctgggctgag aatgacgtct cgcaaggctg aaatcctgag ttaggtagtt
    28681 agcagagcgt ttctttttca agtccgcacg cgcttcttca cgcgccacct ccgcatagct
    28741 ctttccttct tttttaaccc ctaaaggagc ctttttaggt ttttcgactt gcttttcaat
    28801 cggttttact ggtttttctt cagtaatagg agcccattct aaataatttt tatctcgata
    28861 ctcacccttg atattactga tcagatcaga ctcatcatag agattcatga ctggcatttc
    28921 agtcaacatg acctcgtcat ctgacaccaa tggaaatcgt tcttgtttca ttttctattt
    28981 cctttcaaca cttcattata gcgtattgtc ttgatttttc aagtgctggc ttcagaaatt
    29041 cccaaaattt ctctaatttc tgctagggtc agactaccac gtgactctgt gccatccaat
    29101 acttgtgaca ccagatgttt cttttgttct tggagttcct gaattttttc ttcaatggtt
    29161 cccttggtca ccaagcgata gacctcaacc gtttcttcct gacccatccg atgggcacgg
    29221 ccaatggctt gcgcttccac cgcaggattc caccaaaggt caaccaagat cactgtatct
    29281 gcacctgtca ggttcagacc gaccccacca gccttgaggg aaatcagaaa ggcatctctt
    29341 tctccttggt taaaggcctc ggtcatgtct tgtctttcct tggctggggt tgaacccgta
    29401 attttaaagg atgtcaggcc caagtctggc agttcttgtt caattttttc caacattccc
    29461 ttgaactgag agaaaatcaa gacacggtgt ccgccgtctg ccacctgtac cagtaggtct
    29521 cggagactat ctagtttgcc gctggctccc tgataatctt ccataaacag ggcaggagtg
    29581 tcacatattt gacgcaagcg catcaaacca gataaaattt ccacacgact tcgctgaaat
    29641 tcctgttctg acacttgagc cagatggtct cgcatctgtt gtaactgggc aaggtaaata
    29701 gccttttgct ggtcttccag ttcattttta taaaccacct caatcaagtc tggcaattca
    29761 gtcagaactt cttctttctt gcgtcgcatc acgaaaggct tgataaactg agccactcgc
    29821 tctgctggca atttcataaa ttctttcttg cttggcaaaa gtccgggcat gacgatttga
    29881 aaaatagacc acaactcacc cagatggttt tcaatcggag ttcctgacaa ggcaaagacc
    29941 gacggcacca caaattgtct caaggtctgg gcaatcttgg tctgggcatt tttcatgacc
    30001 tgagcttcat ctaagaaaag gaagtcaaag gccatcccct gataaagctc actgtcctga
    30061 cggaaggtgg catagctagt cacatagatt tgatggctct cggcaagaat ctcctcacga
    30121 cttgctttca aaccatgaac aacagccaca tccaactgtg gagcaaattt ctgaaactca
    30181 tctgcccagt tgtaaatcaa acccgacgga gcgagaatca aaacccgact ttcttttgtc
    30241 acttgactag tcaaaaaagc aatggtctga agggttttcc caagtcccat atcatcagcc
    30301 aaaatcccac caaaaccata atgatggagc atctgcaacc agccaattcc cttttcctga
    30361 taatctcgca agtcagcctt gacctgagtt gcttgcaaag gaaagtcctc tggatgcgtc
    30421 aaatcctggg ccagattctg gaattcttgt gaaaaagaaa cacggtctcg cccttcaaag
    30481 agatgagcta aactgtaggc caaggatttc cgagcctgca aggtcccatc ttttaattca
    30541 aattgcccca gttcctgtag attttggcga attttcttgg tttcttcatc gaaaaagtaa
    30601 acttgattag acgaatcaat ataaaaatcc tgattggcaa ccaaggcctg catggcttgg
    30661 tcgatttcct cctgggcaat attttgaaaa tcaaactgga tttccaagag acctcccttg
    30721 gaggcaatct gcacctgagg actcgctagg ctataaagct cttctagttt atctgatagg
    30781 tcaacatgcc cgagtttttc aaagactgga atgatatcat gaaaaaaatg atagacagac
    30841 tccgctttta aggcctgacg ccaagattga aaatcggctt caaagcccgc agccaaacag
    30901 acttggaaaa ttctttcttc taagtctgcg tcacttgaaa agggtaattc ttctagctct
    30961 tgtcggctag atacctgtct atttccataa tcaaactgaa tttctaaacg aatccgatta
    31021 tcttcttccc tgtcaaagta aaaagagggc gcaaaagttt tgatttgtag acgttctgga
    31081 gctgaaacgg tgcccatctg gataaaaaga gtcagacagg aggccaattt gtctcgatca
    31141 ctgctatcaa attgcaggta tttctttcct tgttgaccca caggtaacgc tttaatttcc
    31201 ttgagaagac gcatctgctg gtctgttaaa aaataaacct gacctttatg gaaaagtact
    31261 gctccctgat aaaagacatt gaccctagga ctctcactga tttccatttc aaaataatcc
    31321 gagtattctg ttactgcaaa ggcaaataga ttggcatcag catgcatatc ctgaaaaagc
    31381 agggtttggt agctatccac ttgatggtca aattgaaaat ggggcaaggc catcagtaaa
    31441 ttcacaccct gctcaaaaaa ggtcagaggg aaaaagaggt gccgaccttg gttttggaaa
    31501 aagaggtctg gaaccagccc ttcctccgtt agtccgtgca agaaagtcaa aagttcttgg
    31561 ctggcatcat caaaggcttc ccaagaaaga gactcctcat aaatcttgcc aatcatatac
    31621 gactttctct gctcgacaat ccttaaaaaa agtggaatat cccgaatgat atagtatttt
    31681 tggctattga tttggccgat tctcagagtc cacaagatat gattggttcc tgcttccacc
    31741 tgacccacag ctgataactc ataggcgcat tctgattttg gagataaaat tcgatccaaa
    31801 aacttgccac ccaaggtcac cttggtttca acggcctctt tttcttcatg accttcttcc
    31861 agactccaca agatttcctg accacgctca tcatttttca gaaaatgctc tagcgctgcc
    31921 aaatgcacac agtagcccct cttttgaaaa aaatcacagg cacaaaaaac caaatcatcc
    31981 tctaaactat agcgcagttc ttcttctgca acgcgagcgt agagccgatt gttcttttcc
    32041 ttgatgatat caaccttacc agtttcataa agggcaacac cttcgatacg aattttcccc
    32101 ggaatcaatt tagccatatt ttcaccttta ccttatcttt ttattatacc atattttcgc
    32161 ctatgaaaat agccttctag gaagactttt ctcctagaag gctggatttt taacatttgg
    32221 caaaagtagc cacaatccgc tgacagactt cttgcaacag agatttgggc atagctatat
    32281 tgatgcgggc atggagactt ccttcctctc caaaatccaa accacggttg aggataacct
    32341 tggcttcatt tctcaacaac tcttgcaatg tttcatcagt caggtcatag gctgaaaagt
    32401 caagccaaat caagtaggta ccttgcggtt tcatgacctt gattttagtc tcttttccaa
    32461 atagatccac cacataattg atgtggtctt caaagacttg cttgagttcc tctagccaat
    32521 ctttaccgta tcgataggca gcttctgtcg ccaaataacc caagcctgaa atttcatgct
    32581 gattattggc caacaggcgt ttctggaaag ccagtctcaa cttaggattt tcaatgactg
    32641 cataggaatt ttttgttcca gcaacattaa atgttttagt ggcactgctc aagacgatag
    32701 caaaattttt gaaggcagga ttgatggtat tgaaagactg gtgtttgtga ccaaagaggg
    32761 tcaaatcttg gtgaatctca tccgaaacta acaaaacacc gtgtttttgg cagagttggc
    32821 caatcttctc caacacttct ttttcccaaa cacgtccacc aggattgtga gggttgcaaa
    32881 gaacatagag tttaacctcc tcttccacca aatccttttc aagttggtca aagtcaatct
    32941 caaacagact atccttttcc actaaggaat tagtaatcaa tctacgatta ttcaacttga
    33001 cactgcgagc aaagggtggg tagacaggcg tgttaattaa aaccgcctcg ccttcttttg
    33061 taaaggtttg aatagctgtt gagatggctg gtaccacacc ctcgataaag acaagagcct
    33121 ctttgtcaaa gttgtaaccg tattgtgtag cttcccactt ttgaacttcc ttaattaagt
    33181 cttcactggc ataggtataa ccataaacca gttggtctgc gtaagtttgc acggcttggt
    33241 ggatttcagg caagactaca aagtccatat ccgctatcca agctggtaga acttcactat
    33301 ccgtttctgt ttctttccat ttataggtat ggtgccctaa acggttgggc aggcttgtaa
    33361 aatcatattt tcccatcttt gtcttatcct tctaaggctt ggcgcaaatc tgcaatcaaa
    33421 tctctagcat cctcaatccc aatagacaaa cgcaagaggt catctgtcaa accataagaa
    33481 tggcgtactt ctgctggaat atcagcatga gtttgagtcg ttggataagt aataagactt
    33541 tccactccgc ccaaactttc cgcaaaagag aagaccttga gactgttcaa aatatgagga
    33601 atgcgtgttt catcggctac tttaaaggaa atcatgcctc cacgaccagt gtagagaact
    33661 tccttaactg ctggagaatc cttcaaaaag gcaaccactt cttgggcgtt agctgttgag
    33721 cgctccatac gaagagacaa ggtcttgaga ccacgaagca actggtagct gtcaaatgga
    33781 gacaagactg cccctgttgt attaagattg taaaaaagct tctcgtatag ttctaaacta
    33841 ttggtcacaa ccactccagc caagacatca ttgtggcctg ctagatactt ggttgctgaa
    33901 tggagaacga tatctgctcc atcttcaatc ggacgttggt aaatagggct atagaaggta
    33961 ttgtccacca ccactttgga acccttagca tgagccaatt ttgctagttt ttcgatatca
    34021 aattccaaca tcaagggatt ggttggggtt tcgatataga gaacatccac atccttttct
    34081 aactcggcaa tcaactcttc ttctgtattg gcataggtaa aatggaaatg accttcctgc
    34141 tccacttggt taaaccagcg aaaagaacca ccgtaaagat cacgcactgc caagacctta
    34201 cttcctactg gaaagacgct aaaggccagt acaatagctg acatccctga gctagtcgct
    34261 agggcatagt ctgctgactc aatagccgcc aagacttcct cagccttact acgagttgga
    34321 tttttagtgc gcgtatagtc aaacccagta gatcgaccaa actctggatg ctgataggtc
    34381 gttgaaaaat gaagtggtgt caccaaagca cctgttgcct catcagactt gatccctgct
    34441 tgtgctaaaa ttgtgttaat gtgtaattcc ttgctcatac aattcctcca aatctatagt
    34501 aactattgta ccacttattt tgtatccttc gttttcttgt tttcaagagc tagttatagt
    34561 ttcaaactat ataaaaaggg agtttttcct gctcccttta atagactata aaatggtgaa
    34621 tctcaaaaga caccttcact ctatcatttg ctcctgcaca aaacgagcat aacgctcatg
    34681 attttccagt agttccttat gagttcctga gccagtgatt ttcccctcct ctaagaagaa
    34741 aatacaatcc acatctttta ccgttgacaa acgatgcgct ataatcacaa ccgtcttctc
    34801 ctttaataca gaatagaggc tactgataat cgcatactca gaatccgcat caagattagc
    34861 agtggcttca tcaaatataa gaatttcagc atcttttaag taggctctag ctatttgaag
    34921 tctttggctt tgcccccctg acaagagtcg tccgcgttca ccaacttcag tatctagtcc
    34981 ctctttcatg gagcgaatct catcacctag tgatactaag tctagcactt tcatcaattc
    35041 atcatcagtt actaagcgat tcaaaccgag acaaagattg tcacgaatac tgccagataa
    35101 gactgcatta ttttgtgaaa cccaagcgat tttacttctc cattctttta agttaaaatc
    35161 atatatactt gattgctcca ttagaatatc tcctgaaagc ggtttataaa accgctctaa
    35221 caaacgcaca atcgttgatt ttcctgatcc agatggtcca acaaaagcaa ttttttgccc
    35281 cttgaaaatt gaacaaataa tatcctttaa gacaggtcga ttttcatcat aaccaaaata
    35341 gacatggtta aaattcaacc ctcgtcctga taccgatttt cctccctcaa atttttcttt
    35401 aggaactgca agcaagttct ccagtgcaac tgaagatccc ttgctcctag aataaacagt
    35461 tacaaaatta gctatattac taataggatt aagtaattga aagaggtaaa tcaaaaacga
    35521 aaccaaggtt cccacagata tatatcctgc gctgacccga taacccccat aggttagcat
    35581 cacagctata gtcgcaaaga taaataagag agcaaacggg gtctcaaaag aagtaatcct
    35641 atctgatttc agtgaattgt tttgtaccct ttcaatacaa ttatccaaaa catcctgtac
    35701 acttttctct gcttggttag tcttaattaa ttcatgttct tgaatctttt cagtcaattg
    35761 ccctgttaaa aatccgattt cctcctgtaa acgacgacta tacttttcac tgatattgga
    35821 aaggggcaag ataataaaca tcatacaagg aagagtgatg aataaaagta gagaaagatt
    35881 ccaatcaaga ctaaataaga ctacaatgga accaagtacc ataactaaac tcagaataat
    35941 atttgggaaa gtcgtaatta aaaactcacg aatgacactc gtgtcattga caatggcaga
    36001 agtcaactcc ccactttggc tcttatcaaa gaaggatttc tctgcataaa tcaacccctc
    36061 tatcactttt ttcctgattt ttgctatctt ttttcacccg attgactaaa cagatagtaa
    36121 ccaatagaag aaaacaaggc ttgaccaata aaaatcaaaa acgattgaaa tactttggaa
    36181 cctatatttt caatagaact cccatctatt aaacctttta agataagggg aagcaacaaa
    36241 gcaagtagac tagacagaac aaggaagaaa ctccccataa tcaccttagt atctactctt
    36301 aataatttta atttcataaa tactccttat aatatttcaa cggataaagt cgggaataac
    36361 tcaatttgag gataaaatct aataaatctt cctataacaa aacgcataac atctaggatt
    36421 ttatatacct gatattatgc gtttttaagc acaaagactt cttacacaaa cttatctaca
    36481 attagatttt atttgacatg ttttgccaat tcttcttggg cttttttatt ggattcttct
    36541 ttttctttca accatttttc tctggctttt gcatattcgt ctgttgtgac aatcttatct
    36601 tgtactttga ggtatttata tgattcaacc ccttttgtac cggttaaacc ataggcagca
    36661 gcaaatggta cggttcttct caatgatggt gttcccccac gcgaaacact tggaagaact
    36721 aaagaactat caatcaacca agcttgaata tcagcatatt tctcataacg tttggctgga
    36781 tcttgctctt tattagcttc ttccaacatt tgagtataga catccagccc aacttcctta
    36841 gccttgtcat tggcttctcc aggctctagt ccaagatttt gtagaactcc tccactatta
    36901 gtattaaaaa tatcgagata ggttgacggg tcttgataat caggtcccca accgccatga
    36961 tataaatcat agtctttctg agcagctgtt tgagcaaagt agcctgaact gtcaaactca
    37021 tctgatgtta attgctgaat gtcaatcact acattatcag aacctaaaac agattcaatt
    37081 gattgtttga tagaactaac tccttgtatg cctactttat ctgttacttc cacagtctta
    37141 tccaagtgga ttgggaattg aacacccttt gcttcgagtt ctttcttagc ttccgcaaac
    37201 ttagccttgg ctttctcagg attgtagtaa gggtcttgac catccgcaaa gttgatacct
    37261 tgccattcct taccatagtt gaccatctta gaggctacaa cttcaccaaa gtcttttccc
    37321 ttgatactga caaagtttgg aggaaccact aggttacgca aaatctttgt tgcaccttct
    37381 ttcccttcag actgagcccc ataagatgtt ctgtcaaaag caaaattgat agcctgacgg
    37441 aagtttttat tgagaactgc ttcctgagtc gatttctttt caatgtcact tgttttagaa
    37501 gtataattgt aagacttcct atctaggtta aaattaaaga aatatgaagt tgaattttgc
    37561 atactataga tgatattgtt tttgtatttt tctttaatcc cttcatagct ggagctgtta
    37621 ggaaaaagac gagccgtagt ataagcacca gctgtaaaat tacgttccag tgattcttga
    37681 tcgctaccat catagtaggt caatttcaca tcgtctacaa agacattctt agcatcccag
    37741 taattagggt ttttcttata ttcaatagca gattttgaga caagtgcttt catcaagaaa
    37801 ggtccattgt acaaaatact agatggatcc gccttcccaa aatcatcccc ttttgatttc
    37861 aggaaatctg cattaacagg aaaaagtatc gttgcaagtg tttttgaatt ccagtaaagt
    37921 tctggtttaa ccaaagtata ttgaaccgtt tggtcatcaa gtgccttgac accgacagtt
    37981 gaaaagtcgc ttgttttacc agtgatatag tcatccaaac cagcaacaga gtcctgcact
    38041 agatacaagg cttctgattt tttatcagct gcatattgca aacctgtcac aaaatcctgg
    38101 gcagttacag gcgcatattc ttctccctca gaagtaaacc acttggcatc cttacgaagt
    38161 ttgtaggtat aggtcaaacc gtcctgagaa acagtccaat cctctgctaa tgatggaata
    38221 atattcccat attggtcatt ttctaataac ccgtctacca aatttgcaac aatatcggat
    38281 gttgctgcgc ggttttctgc tagatagttc aagctagatg gatcacttga ataaacatag
    38341 ttgtaggttt ttgacgccgt gctagaattt ccacacgcgc tcaataaaac tcctgtaccc
    38401 aggacaagac ctgccaaggt tagatatttg ctcttagact ttttcatttc cagaacctcc
    38461 tgctttaaat atttgtttta ttatacaacg tttggtttgt ttagtcaatt atttttccaa
    38521 aaaatcgaaa aaaacctcat tttctatttt tgaaagataa ttttagctat ttttacgcta
    38581 aaaaaggaat aagtttcaag ttctcctaaa agattgttca acttattcct tttatttata
    38641 aatgctacct tacgaaagct taaattttgc tcgcaggcga tctgcttggg cttttcctat
    38701 taccttatct aataaatagg tacgcagact cataaccata taaagtccac cccccatggc
    38761 accgacaaga gctacataaa agaagctcca caaacgtcca cttggttgga agaaaaatcc
    38821 taacagccac tggatggttc ctattaacag aaacatgact agggtcagca aactgattaa
    38881 aatggttcgc ttcaaaatca ccttgcgctt gacaccagtt actttacaaa tatcccgata
    38941 catcaagacg ttaggaatga tgagagcaat ggttgttgaa atcaaaggac cataactgtg
    39001 gaagagggcg atggtaggta gttgcaagac tagcttggca atagaaccat agataaaata
    39061 gagaacggcc ttgcggttgc ggaacatggc ctgaagcatt ggagacaaga ccatgtacaa
    39121 gcctaaaata atagactgca aaactgcaaa gacaaataag cccagagcca aactatctgg
    39181 cttaccatag aagaccgtat aaagaggttc tcctaccata accactccaa ccgttgctgg
    39241 tagcaagaac ataaagagta gggtgagact gtcctgaacc agacgagaag ctgctttcaa
    39301 gtcccccttg acatagtttt ccgtcaaaag tggcaaacca acactcccaa tcgaaacccc
    39361 tacagaaatc aaaatcatcg tgattttatt aggattggct gagaaataag aaaacatgac
    39421 aaccaagtcc tcattgctgt agttggtaaa ccagctcata ctattgataa aggtcagctg
    39481 atccaaaatc tggaagagct ggatggcaga ccctgtcagg ataaaaggaa tggcttcctt
    39541 aatggtatca accaagagac gcttactgtt aatcttatct cctgtttcaa agactctttt
    39601 gagtgaacct tcttgggcaa ggaaataaat caagactgca aaactggcta ccataccgac
    39661 aaaggcagca aaggttgatt gggtaacggc tgctagataa tctcctgaac cgagcttcat
    39721 aatgataaag gttgctagga gcatccagat aacacgaatg acctgctcag caatttggct
    39781 catggcatag ggtttgaggt tattcatccc ttggaaaaat cctcggataa cactcataga
    39841 cgggaaaatc aagactcccc aagccaagct ttgcatgatt gggatcaagt ctttgcccac
    39901 gccagacaag tctgctagcc aaggagcaaa gacatacaag actaaagcaa aaaccaggcc
    39961 tagtcctgtc ataaagccta agaagctccg aatcagggca aagctatgct cttcttctcg
    40021 catggtatta tacttggcaa cttgcttggc caccgcaact ggaatccccg ctgttgaaac
    40081 caacaagaac caagcataga tattgtaacc catggtaaag agaccatttg ccttagctgc
    40141 ataagccccc atccagatgt accaagggat aatgtaaaca gccccgagta ggcgactgat
    40201 aaagttacta gccgttagcc aagcagtccc ccgtaacatc tgggcctgct ggtgattgtt
    40261 ttcgtgcgac atagattcct cattcaattt tgataactag gaaaaactcc ttatcttcca
    40321 ttataaactt ttccttgtta cttgtaaatt tgcgactttc ggtttacaat agaaagtatg
    40381 attaagattg aaaccgtatt agatatttta aagaaagatg gcctttttcg cgaaattatt
    40441 gaccaaggtc attaccacta caactacagc aaagttgttt ttgatagcat cagctacgac
    40501 agccgaaaag taacagaaga cactcttttt tttgcaaaag gcgctgcctt taaaaaagaa
    40561 taccttcttt ctgctataac acaaggttta gcttggtatg tagctgaaaa ggactacgaa
    40621 gtcgatatcc ctgtcatcat tgtgaacgat ataaagaaag ccatgagttt gattgccatg
    40681 gagttctatg gtaatccaca agagaaactc aaactccttg cctttactgg tactaagggt
    40741 aagacaacag caacctattt cgcctataac atcttatctc aagggcatag acctgctatg
    40801 ttgtcgacca tgaacacaac tcttgatggc gagactttct ttaagtcagc gttgacaacc
    40861 cctgagagta ttgacctctt tgacatgatg aatcaggctg tgcaaaatga ccgtacccac
    40921 ctcatcatgg aagtctccag tcaatcctat ctggtcaaac gtgtctatgg tctaaccttt
    40981 gatgtgggag ttttcctcaa tatcagccca gaccatatcg gcccgattga acaccctagc
    41041 tttgaagact atttctacca caagcgtctc ttgatggaaa atagccgagc agtcatcatt
    41101 aacagtgaca tggaccactt ctcagtcttg aaagaacagg ttgaagatca agaccatgat
    41161 ttctatggta gccaatttga taaccaaatc gagaattcca aagcctttag cttttcagct
    41221 acgggtaaac tcgctggaga ttatgatatc caactcattg gcaacttcaa ccaagaaaat
    41281 gcagttgctg ctggacttgc ttgtctccgt ctcggagcaa gtcttgagga catcaaaaaa
    41341 ggcatcgctg caacccgcgt tcctggtcgt atggaagtcc tcactcagaa aaatggagcc
    41401 aaggtcttca tcgactatgc ccacaatggg gatagtctga aaaaactcat caatgtggtg
    41461 gaaactcatc aaaccggaaa gattgctctg gttctgggat caacaggaaa caagggagaa
    41521 agtcgtcgta aggactttgg cctcctcctc aatcaacacc ctgagattca agtctttctg
    41581 actgctgatg accctaacta tgaagaccca atggccattg cagatgaaat tagtagctac
    41641 atcaatcatc ctgttgaaaa gattgcggat cgccaagaag ccatcaaggc ggcaatggct
    41701 atcacaaatc acgaattaga tgcagttatt attgcgggta agggagtcga ttgttaccaa
    41761 atcatccagg gcaagaaaga atcctaccca ggagatacag ccgtcgcaga aaattattta
    41821 taagaataga aaaaatcaag ggaaaatgaa acccttgatt ttttcctatt atttagtaaa
    41881 aacgtttttc aaaccttcta cagcaccttt tgcgtcttct acaacgtctt ttgccttagc
    41941 aactgttttt tcaacagctc cttcaagttc tgtcttgctg tctccagtaa ctttaccaaa
    42001 accttctttg acagcgcctg ttgcttgttc caatttgttt tcaagtgaca tatgattgtc
    42061 tcctttagtt tattccgata tgtgttaccg gttacatata gtttataccg aatactaagg
    42121 aaagtcaaac aatgtactca gaaacaagaa atcacgctaa gtagaaagtt aatctctcct
    42181 tatcaaaatc gatagccaac tcatactttc catcttcatt ttgcacaata tagcctaaga
    42241 caaccaaact gtccacaaag atatcacggc atttctgcat aagctggtct tttttgagaa
    42301 atttcaacaa aaaagccgtc atatacttga gggcatactc aggattaaca tctcctaaaa
    42361 tgtcatagag ctcctgctgt ttttctgtca aaggatactg ctgtttgacc ttgtaaaaat
    42421 aattggacag ggtcatttta atgcgcgcaa agtccgtctt ttcaactaaa atagctgcat
    42481 tgatttgatt gcgcaattcc gtctcaaaac tctgctccaa caaggcttga tagaccggac
    42541 tagcttctct gacaaaaatc tcttgatcca gttcaagact atcaagtgat tcaagcgtag
    42601 gaaaattaag gtaatagcgc ttattttcac gtagaatcaa gcttgccttt atatactctt
    42661 ccatgagttt atcaactgca acatctggaa attgagcctt aatttcccgt agaatcacat
    42721 cctcatactg gtccagatag cggatcaatt ctccaaaaat ggctgtctcg tcaaacgaga
    42781 tggattaaaa atctgaatca tgaagattcc tttctttaca ttgtaacaga ggcgatgctg
    42841 ccaactgact aggattggtc ccagcttggt tcagaggaac aggcagacct agttctatgt
    42901 aatactccct ccaaaaatca ctaatctcct gttcctcatc tccaaatttc ataggaatag
    42961 tttcaaaaat aggtaggatt tctgctatat actgggagca gtagaaacca gctgcatctg
    43021 gatagaaaga agcattgtag ggtgctccaa gatgtctgca agctctttcc ttcaccgact
    43081 ggatatccat ttctgggtaa acatagaggt cgtacaaatg attggactca aagaagtctg
    43141 ccggttcttg acagacaaca ccagcctgtc cactagcatg ataaatcatc ccatccaaat
    43201 aaatggcaac atggctatag ttacctgtgg aagtctggat ggcctgtccc atgtctgacc
    43261 catctctcac aaaaatcaaa tcgccatttt ctaacatact tcactcctag aaaaaaagga
    43321 gccgaaactc ctttcgatat aaggcaaact agactttccg attttgagct gacacccaaa
    43381 atcttacgca ttaaagcttt cagttaattg aggtaccact tgtttcttac gtgaaacggc
    43441 accagcaagg aaggcatgat tgttttcaag tttgaaattg aaagccgctt cgaccttgtc
    43501 catattggca ccaagagcca atatttctga gtttgagttg acgatatctg taatcatcaa
    43561 gacaaagtca gagtagccgt ttgattcgtt ggcagcttgc attgcagctt caatttctgc
    43621 ttggcgttcc aaaacttcag cgatgtcaac tgtgttcact tgggcaacac ggacattatt
    43681 tccgttgagt tcaaaagtct tagcatcgat gtcaatcaat tcttcagcag atttgctagc
    43741 caagttggta ccagctttca acattgccaa accatattct tccaagttca caccagccaa
    43801 ttcagccaat tcaggagcaa tgattttatc tgttgggtgt gttgttggtg atttcaaaag
    43861 aagggtatct gaaatcaaac ctgaaagcat caaacctgca atctctttag gcacagctac
    43921 accatgttct ttgaacatac ggtaaacgat tgaagacgct gatccaactg gctccaaacg
    43981 catgtaaagt gggcttgcag tttcaaagtt agccacacgg tggtggtcta caacaccgta
    44041 aacttctact tcagcgatat ctgatacaga ttgttggaat tcattgtggt cagtcaagat
    44101 aacttgctct gccccctctg ctttggcaga agtgataaca cgtggtgctt ccacaccaaa
    44161 atagttcaag acaaaggctg tttcttcatt tggagttcca agggcaacag cttccgtatc
    44221 caaaccgtaa gcttcttttg caaggtaggc aaaagctaca gatgatccga tggcatctga
    44281 gtctggattt tggtgaccaa atactagaat cttggacatg ataatacctc attttgttta
    44341 ttttctttat tgtagcattt ttttcgcttt ttgacaaagg taagaggagt caaaggactc
    44401 cccacgattc ttcaaaataa ctgagtaaat ttctatcttg attttcagat aaaaattctt
    44461 ccttctgtgg cctcttctta cgcttgagaa gagcttcagc cgacatggct tcttccttac
    44521 tggcaaaacc ttgagcatag ataagtttga ctggcaagcg tgctcgtgta tatttggctc
    44581 ccttcccact attgtggata gcgaggcgtc ttctcatatc agtcgtatag cctatatagt
    44641 aggatccatc acgacactcc agaacgtaca tataagcctt atgatccata ataaatctct
    44701 tcgatttcgg gcgtataaga gccatcatca ttgtggacaa tcaaaggagg taagacctta
    44761 aagccacttg ttgagccatc cttgatcgcc tcaatcaaaa gcatattggc ttccttttct
    44821 ctttttggat aaacaaactg caggcgctta ggggctagat tatgtcgttt taacgtatcc
    44881 aaaatatcca gaagtcgatc aggacgatga accatggcca aacgcccatt agacttgaga
    44941 atactctggg cactatgaca gatttcttcc aaattagtcg tgatttcgtg tcgagccaag
    45001 agataatgtt cactctcgtt cagattagaa taaggatcca ccttgaaata gggtggatta
    45061 cacaaaatca tatccacctt actcccctga atgtgagcag gcatattttt caaatcatcg
    45121 cagatgacct gcatttgctc ctctaatcca ttcaaacgga cagagcgttc agccatatcc
    45181 gccaaacgct cctgaatctc aacagacaat atctgtgctt gagtacgagt gctagcaaaa
    45241 agccccactg ctccattccc agcacagaaa tccacaatca accccttctt aggaaaacgt
    45301 ggaaatcgtg ataagagaac actatccacc gaatagctaa aaacctctct attttgaatg
    45361 attttgatat ctgtcgaaaa gagctggtta atgcgctctc ctgattttaa taattgttct
    45421 tcttccatgg tcctattata gcaaatttat attaacatta caaaaaatat aaaactctaa
    45481 actacttctt cttttttaaa tggtgcaggg cttctccagt ccagattggt agcattcgtc
    45541 gaaagggagc aaaaccgtag ttaaagcggt cgcttgaaaa gcgtctccgt ctaggaaact
    45601 ggtacttttc ttcctccaaa gtgcggatag aaagactggc tttccccgta aattcatcta
    45661 aatccactac ctgaacttga acctcttcat cgactttcaa ggtttcatga atattttcaa
    45721 taaatcctgt ccgaatttct gaaatgtgaa tcagccccgt atcacccgtc tctaactcaa
    45781 caaaggcacc gtagggctga atccctgtaa tacgcccctt tagcttatca ccgattttca
    45841 tcttagtcct cgatttcaat agtttcaatt acaacatctt caactggctt gtccatagct
    45901 cctgtctcaa cagcagcaat ggcatccaag acagcgtaag atgcttcatc agctaactga
    45961 ccaaaaaccg tgtgacgacg gtctaggtga ggtgtcccac cttgattggc atagatttct
    46021 gcaatcggtt ctggccaacc accacgagta atttctttct tagaataagg taggtgttgg
    46081 ttttgcacga taaagaactg gctgccgttg gtatttggac cagcatttgc catggaaaga
    46141 gcaccacgga tattgtaaag ctcttctgag aattcatcct caaaagattc gccgtagatt
    46201 gactcgccac ccataccagt tccagttggg tctccacctt ggatcataaa gtccttgata
    46261 atacggtgga aaatgacacc atcatagtag ccatcttttg aaagagatac aaagttagcc
    46321 actgttttag gagcatgttc agggaaaagc ttgatacgta agtctccgtg attggtctta
    46381 atagtcgcaa gaggaccttc tactgtttca atgtctactt gtggaaaatg caattctttt
    46441 tctaccatac caaatacttc taaggcatca aaaatgccat cttcttctaa tgtttttgta
    46501 atataatctg ctttttcttt gattttatca tgagaaattc ccatggcaac gctgattcca
    46561 gcataatcaa agagttccaa gtcgttgaga ccatctccaa aaaccatgac cttctctggt
    46621 ttcaagccaa ggtgttccac aactttttcc acccccgtcg ctttggagcc tgaaatcggc
    46681 acaatatcag acgaatgttg atgccaacga accatgcgaa gtttgtctga gagactgtca
    46741 ggcaagtgca agtcatctcc cttatcttca aaagtccaca tctgatagat atcttctttt
    46801 tcatggaaat cgggatctac atctaagtcg ggataaattg gattgatagc ttcactcatc
    46861 atatcggtgc gagtcgacaa cttggcatca tgactcccaa ccaagccata ctcaattcct
    46921 tcttgcttag cccaagagat atactcctca acatctgact tttcaatctg atgctgataa
    46981 atgacctgac cttttttatc ttcgatataa gccccattca aagttacaaa aaagtcaggc
    47041 ttgagatcac gaatctctgg aacaacacca aaaatgccac gtccagaggc gattcctgtt
    47101 aaaattcctt tttcacgcaa ctgtttaaaa acagtgggaa ttgtagttgg aataaaccct
    47161 gtctttgaat tccgcaatgt atcatcaata tcaaaaaaga caatcttgat cttctttgcc
    47221 ttgtatctta atttcgcgtc catctcacta cctctttcaa tctaactctt tccattatat
    47281 cataaagtag gcaaatcccc tattttcaaa aagtttatca tttttatttt aatttcttgg
    47341 atgagaaaaa agacatattt atgaaaaagc tccatcgtgc ttttaatgtg ttctcttgtt
    47401 ttcaaactcg taaaaaggga gccactgagc ctaactcgct ctctcatttc aaagtttgtg
    47461 aaaaaagacc cattggggtc ttaattcgct ttcttgtttt caagctcatg aaaaagagac
    47521 ccaaccgggt cttttcttta atcttcgttt acgaaaggca tcaaagccat tacgcgagcg
    47581 cgtttgatag ctgttgttac tttacgttgg tttttagctg aagttcctgt tacacgacga
    47641 ggaaggattt tcccacgttc tgaaacgaaa cggctaagaa gctcagtatc tttgtaatca
    47701 acatattcaa ttttgtttgc tgcgatgtaa tcaacttttt tacggcgttt gaatccgcca
    47761 cgacgttgtt gagccatgtt ttttctcctt tataagttta gttgtccatt agaatggtaa
    47821 atcatcatct gaaatatcca atgggtttgt tgctccaaat ggattttcat tacgtgaaaa
    47881 gtctagtact gaatttgtag gtgctgaata gtttgcagtt ggtgcagagt aagctccacc
    47941 tgtgtgaccc tcacgcacac tacggctttc caacatttgg aaattctcag ccacgacctc
    48001 tgtcacgtag acacgttgtc cttgctggtt atcgtaacta cgagtctgga tacgacctgt
    48061 caccccgata agtgagcctt ttttagccca gttagcaaga ttttcagcct gttggcgcca
    48121 cataacgaca ttgataaaat cagcctcacg ttcaccattt tgactcttaa atgtacggtt
    48181 tactgcaaga gtaaaagtcg caactgctac atttgatggg gtataacgca actcagcgtc
    48241 acgtgtcata cgccctacaa gtacaacatt gttaatcata gtttaccttc ttacgcgtca
    48301 attttgacga tcatgtgacg aagaatgtca gcgttgattt ttgaaagacg gtcaaactct
    48361 ttaagagctg catcgtcatt tgcttcaacg ttaacgatgt ggtaaagtcc ttcacggaaa
    48421 tcttggattt cgtatgcaag acgacgtttt tcccaagttt ttgattcaac aacagttgca
    48481 ccgttgtcag tcaaaataga gtcaaaacgt gctaccaaag cgtttttagc ttcttcttca
    48541 atgtttggac gaatgatata aagaatttcg tatttagcca ttgatatgtt cctccttttg
    48601 gtctaatgac cccaagactt tgcaaggggt aagtgaggtt cgctcacaat aaactattat
    48661 actagaaaaa atttttttac gcaagtaaaa acactagaat tcgaaaaaac gccacatggg
    48721 cgttttcctg ttcttatggt ttgatacggt gcaacatacg tgggaatgga atagcttcac
    48781 ggatatgttt tgttcctgct gcaaaggtta ccatacgttc gataccgata ccaaatcctc
    48841 cgtgtggaac tgtaccgtat ttacgaaggt caaggtagaa ttcatattct gtacgatcca
    48901 tgccaagttc atccatctta gcgacaaggg catcgtaatc ttcctcacgc atagacccac
    48961 cgataatttc tccatagcct tctggagcaa gcaagtctgc acaaagcacg cgctctggat
    49021 ttccaggaac tggtttcatg tagaaggcct tgatggctac tggatagttc atgacaaatg
    49081 ttggcacacc aaagtggttt gaaatccaag tttcgtgtgg tgacccaaag tcatcaccat
    49141 gctcaagatg ctcgtagtca gcatcttcat cattttcatg ctcttgcaag agatcaatgg
    49201 cttgatcgta agtgatacgt ttgaatggct ctgcaatgta gcgtttcaag agttctgtat
    49261 cacgttccaa ggtttccaag gcttgaggcg cgcggtcaag aacaccttgt agaagagctt
    49321 tcacataagc ttcttgcaag tcaagcgact catcatgtgt caagtatgag tactcagcat
    49381 ccatcatcca gaactcagtc aagtgacggc gtgtttttga tttttcagca cggaaaactg
    49441 gaccaaagtc aaagacacga ccaagagcca tagcccctgc ttctaggtaa agctgacctg
    49501 attggctcaa gtaggctggc gttccgaagt agtcagtttc aaagagttct gtagaatctt
    49561 ctgccgcatt tcctgaaaga attgggctgt caaacttcat aaaaccgttc ttgtcaaaga
    49621 actcataagt tgcatagata atagcgttac ggatttgcaa cacagctact tgcttacgag
    49681 agcgtagcca caagtgacgg ttatccatca aaaagtctgt tccgtgttct tttggtgtga
    49741 ttgggtagtc ttgagattca ccgatcactt cgatgtctgt gatgtccaac tcatagccaa
    49801 atttagaacg ttcgtcctct ttgacaatac ctgtcacata aacagacgtt tcttggctca
    49861 agcgtttgat aacatcaaac ttctcaagtc ccacttcttc accaaatttt tcgacaaagt
    49921 ttggtttaaa agccacacct tgaaagaagg ctgttccatc acgcaattgt aagaaagcga
    49981 ttttcccttt tcctgattta ttggcaaccc aagcgccaat cgtcacttcc tgaccaacat
    50041 agtcttttac gtcaataatc gttacacgtt ttgtcattat ttttcctttt cttttttatt
    50101 ctttatggca aaccacctct atattgttcc catccaggtc aatcataaaa gcagcatagt
    50161 aaatcggacg ctcacttcga taaccaggag ccccattatc tcgcccacct gcctctaagc
    50221 cagcctcata acaagcctaa acttcttcct tattttctgc taaaaaagca aaatgaacag
    50281 gatcttgtgt tccctgagtc agccaaaaat caccaccagg atgagggctg ttcggggata
    50341 gaaaactaat tagagaacta gtcttaaaag ccaatttata gtccaaagga gcgagaaaac
    50401 tcctataaaa tccttctgaa atttgtaaat cctttacctt aatctcaaaa tgatcaatca
    50461 ttctcactac ccataaatgc tttcaagcgt tcgactgctt ctttaagcgt gtctaggtct
    50521 gtcgcatagc tgaggcggac attttctggt gctccaaatc cagctcctgt taccaaggcc
    50581 acttcggctt cttctaagat agcagttgta aagtctgtca catccgtgta gcctttcatc
    50641 tccatggcct ttttgacatt tgggaagaga tagaaggccc cttgcggttt gaccacttca
    50701 aatcctggca cctctgcaag gaggggatag atggtattaa gacgttcctc aaaggcctga
    50761 cgcatgcttt ctacagtatc ttgctcacct gatagagcct caactgctgc atattgggct
    50821 actgctgacg gattcgaagt tgtttgacct gcaatcttgg acatggcagc gataatgtct
    50881 gcttctccaa cggcataacc aatccgccaa ccagtcatgg cataggtttt agacacacca
    50941 ttgatgacca ctgtttgctt gcgaatcgct tccgataggc tagaaatcgg tgtgaactca
    51001 tgaccattat aaaccaagcg gccatagata tcgtctgcta ggatgagaat atcattttct
    51061 acagcccagt ttccaattgc caagagttcc tcacgggtgt aaatcatacc tgtgggatta
    51121 gatggcgaat tcagcaccaa aaccttggtc ttgtcagtgc gagctgcttc taactgctct
    51181 acggtcacct taaagtgatt gtcttcctta gcagaaacaa agacgggaac gccttctgcc
    51241 atcttgacct gatctccata gctaacccag tatggggttg ggatgatgac ttcatcacct
    51301 ggattgacca cagccataaa gaaggtatag agagaatatt tggctcccgc agcgactgtc
    51361 acttgatttg acgctacaga atagccgtaa aagcgctcaa agtagctatt gaccaccgcc
    51421 ttaagctctg gcagacctga ggttactgta taaaaagaag cacgcccatc tcgaatcgat
    51481 gcaatggcgg catcttggat atttttggga gtagtgaaat ctggctcacc caaggttaga
    51541 gacaaaatat ctctgccctc agccttcagt gctttggcac gggctccagc agccaaagtc
    51601 acactttctt ccatttctaa aacacggttg gataatttca taggccctcc ttgttgacca
    51661 atgctcctgt ttcaaaatct actagataaa aatcagatcc tgacttgact tcccagattg
    51721 gcttatcttg ataacgacca aagataatct tgtcaatttc gccagctccc ttttccttag
    51781 aaaccgtttc tgctttttct tgtgaaacac cctgatttag ctgataaacg taaatcttat
    51841 gatcatcttt tccaatcaga acagcaagtg cttcttgctg tttattacga ccaagaacgc
    51901 tgtaataaga ttccaagcca ttgtataaat caacctgatc ggcctgctct aatcctgcat
    51961 actgctgagc taatttttct ccttcacttt tagctgtttg atagggtttc atgctaagag
    52021 aaaccatata cagaaaggaa gcactgataa ccacaaacaa aatcgtcatc cctagaccat
    52081 actgccacag tagattattt tttgctttgt tttgtctttt tttcactcgt ctattttacc
    52141 atctattaag ctttattaca agtgaatata agaatactct tcgaaaatct cttcaaacca
    52201 cgtcagcttt atctacaacc tcaaagctgt gctttgagca accaattcta tttctccctt
    52261 caaacaaaac cgattttgaa agtgaaacag ttcttacttt ttcagtcaca aatgactaga
    52321 gtttccgaaa ttttacaatt ttcaaataaa ctctgagcaa atttcttgca agttcttttg
    52381 ttttgttgta atatatttta taacaacgag agagttctcg aaattttaag aaaaaggaga
    52441 cacatcatgt ctaaaaaagt attatttatc gtcggatcac tacgtcaagg ttctttcaac
    52501 caccaaatgg cgctcgaagc tgagaaagca cttgctggta aagcggaagt tagctacctt
    52561 gattattcag cccttcctct cttcagccaa gatttggaag ttccaacaca tccagctgta
    52621 gctgctgctc gtgaagcagt tctcgttgcg gatgctatct ggattttctc tccagtctac
    52681 aacttctcta tccctggtac agtgaaaaac ttgcttgact ggctatctcg tgcccttgac
    52741 ttgtctgata cacgtggcgt ttctgccctt caagacaagt ttgtcacagt atcatctgta
    52801 gccaatgcag ggcacgatca acttttcgct atctacaaag acctcttgcc atttatccgt
    52861 acacaaggcg ttggtgattt cactgctgca cgtgttaatg actctgcctg ggcagacgga
    52921 aaattggttc ttgaagaaac agtcctaaac tcacttgaaa aacaagctca agacttggtc
    52981 gaagctatca agtaactaac actcaataaa aatcaaaaag caaactagga agctagccgc
    53041 aagctgctca aagcactgct ttgaggttgt agatagaact gacgaagtca gtgacatata
    53101 tacggtaagg cgacgctgac gtggcttgaa gagattttcg aagagtataa cataataaaa
    53161 gcgaacaaga ctacttttct gacactcaga agactagctt gttcgctttt tttctcattt
    53221 ataaactaaa acagctgatg atacatattg ccaaatacaa aatatctgat ttctatactt
    53281 cctttacaaa aaccaatccc tgtggttgtg acaaatcttc tcggtaaaca aatccagcaa
    53341 agttcaagcg acgtgcttcc cccacagttt gtacttgatt ttctattaaa gctgttagaa
    53401 aggccccgcg cgctttcttg gagatagttg agtgaatctt cagctgaccg cctctatcct
    53461 ccatgaattt gaaggtcacc atctttgctc tgatttcctt agaaaataca gtctcaaact
    53521 ctgatgacaa gagagagaaa atcacttctt ccttcttcag agcttcatca tagactgcct
    53581 tccaatggct cttcaaagtc ttaccagcga cttttaattt catcaaaaaa tccaaacggt
    53641 gaggagccat gggtgacaag gctggaacaa caccgtacaa agccgaggta atgaagacat
    53701 gattttcaag ataatcttgt tccgcctcgg tcagcttatc tctcttaatg ttgcggtaca
    53761 taagcccatc aaaaagtttc aaggctggat agtgttgagc agtttgcctt ttcaaagctt
    53821 ggatattttg aaattcttcc gccgctttct cagccgatac cttgtagaaa ctctccaatt
    53881 gactggcaga atagagagcc aaggcatcaa gcacggtctg actttctggt tttaaaggaa
    53941 ttgcctcgat acttggcaag tctgtgttca tttcttttgc tgttgggatt aaaattttca
    54001 tattgatagt gtagcatatt ttttagccac aaccaagaaa ttcatttgaa aaacctcggt
    54061 ttgaccgagg tttttctatt tatttgggcc atcctaacca gacagccacg agaacaaggg
    54121 ctgtgccagc taaacttgtt aagatagata agaatttatc ttttttctcc ttgaactgag
    54181 aaaaacctat cttcaaagcg agcagtccga gtagcattga agcaaccatc aaataaaaac
    54241 ccatttcttt gtcctccatt aggcttaatt taccttatta taacatatat ttattaaata
    54301 aacctt
//
LOCUS       DAVKJD010000006        49945 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKJD010000006 DAVKJD010000000
VERSION     DAVKJD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49945
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.006"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(29..1048)
                     /gene="adhP"
                     /locus_tag="VW157_000366"
     CDS             complement(29..1048)
                     /gene="adhP"
                     /locus_tag="VW157_000366"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9551724.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(1387..2199)
                     /locus_tag="VW157_000367"
     CDS             complement(1387..2199)
                     /locus_tag="VW157_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9551725.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            2389..3807
                     /locus_tag="VW157_000368"
     CDS             2389..3807
                     /locus_tag="VW157_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9551726.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLVITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGATLGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            3860..4567
                     /locus_tag="VW157_000369"
     CDS             3860..4567
                     /locus_tag="VW157_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9551727.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            4662..5636
                     /gene="folP"
                     /locus_tag="VW157_000370"
     CDS             4662..5636
                     /gene="folP"
                     /locus_tag="VW157_000370"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9551728.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            5638..6960
                     /locus_tag="VW157_000371"
     CDS             5638..6960
                     /locus_tag="VW157_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9551729.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLFDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNVTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            6941..7495
                     /gene="folE"
                     /locus_tag="VW157_000372"
     CDS             6941..7495
                     /gene="folE"
                     /locus_tag="VW157_000372"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9551730.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            7538..8350
                     /gene="folK"
                     /locus_tag="VW157_000373"
     CDS             7538..8350
                     /gene="folK"
                     /locus_tag="VW157_000373"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU9551731.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(8395..8712)
                     /locus_tag="VW157_000374"
     CDS             complement(8395..8712)
                     /locus_tag="VW157_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9551732.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            9069..9515
                     /gene="rplM"
                     /locus_tag="VW157_000375"
     CDS             9069..9515
                     /gene="rplM"
                     /locus_tag="VW157_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9551733.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            9535..9927
                     /gene="rpsI"
                     /locus_tag="VW157_000376"
     CDS             9535..9927
                     /gene="rpsI"
                     /locus_tag="VW157_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9551734.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(10189..10614)
                     /locus_tag="VW157_000377"
     CDS             complement(10189..10614)
                     /locus_tag="VW157_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9551735.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPTMAVGEFALQSLKQ"
     gene            10761..10937
                     /locus_tag="VW157_000378"
     CDS             10761..10937
                     /locus_tag="VW157_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551736.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            11338..13551
                     /locus_tag="VW157_000379"
     CDS             11338..13551
                     /locus_tag="VW157_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9551737.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFATEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(13773..14249)
                     /locus_tag="VW157_000380"
     CDS             complement(13773..14249)
                     /locus_tag="VW157_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9551738.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            14472..17675
                     /locus_tag="VW157_000381"
     CDS             14472..17675
                     /locus_tag="VW157_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU9551739.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVSIEAKKKYKLRFKIKTDHKVGIAKVRIIEESDKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDTYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISVSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVGRATFNQMIKELESSLIDNNETLQSVYDAKQGVWGIVKYDDSVSAISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            18055..18440
                     /gene="tnpA"
                     /locus_tag="VW157_000382"
                     /pseudo
     CDS             18055..18440
                     /gene="tnpA"
                     /locus_tag="VW157_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(18819..19448)
                     /locus_tag="VW157_000383"
     CDS             complement(18819..19448)
                     /locus_tag="VW157_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9551740.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDALTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(19458..20459)
                     /locus_tag="VW157_000384"
     CDS             complement(19458..20459)
                     /locus_tag="VW157_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9551741.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(20490..21131)
                     /locus_tag="VW157_000385"
     CDS             complement(20490..21131)
                     /locus_tag="VW157_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9551742.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(21150..21965)
                     /locus_tag="VW157_000386"
     CDS             complement(21150..21965)
                     /locus_tag="VW157_000386"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9551743.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            22237..22671
                     /locus_tag="VW157_000387"
     CDS             22237..22671
                     /locus_tag="VW157_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9551744.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            22683..23873
                     /locus_tag="VW157_000388"
     CDS             22683..23873
                     /locus_tag="VW157_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU9551745.1"
                     /translation="MIKKVTIEEIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELAHHDLGFLYTPSCMAEYKINGDGEAREAALKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            23884..24375
                     /locus_tag="VW157_000389"
     CDS             23884..24375
                     /locus_tag="VW157_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9551746.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            24390..25169
                     /locus_tag="VW157_000390"
     CDS             24390..25169
                     /locus_tag="VW157_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9551747.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            25156..25974
                     /locus_tag="VW157_000391"
     CDS             25156..25974
                     /locus_tag="VW157_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9551748.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            25974..26267
                     /locus_tag="VW157_000392"
     CDS             25974..26267
                     /locus_tag="VW157_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9551749.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            26289..28190
                     /locus_tag="VW157_000393"
     CDS             26289..28190
                     /locus_tag="VW157_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9551750.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHVASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPRSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            28250..29251
                     /locus_tag="VW157_000394"
     CDS             28250..29251
                     /locus_tag="VW157_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9551751.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(29361..29696)
                     /locus_tag="VW157_000395"
     CDS             complement(29361..29696)
                     /locus_tag="VW157_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551752.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(29707..30243)
                     /locus_tag="VW157_000396"
     CDS             complement(29707..30243)
                     /locus_tag="VW157_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU9551753.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALITAGYVTYQMKKTGITAIDPDMLSEKE
                     SKQLHYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSLLNTKHIFKTILGAFFFGV
                     MIHIVDSLRIARAKKDQD"
     gene            complement(30259..30453)
                     /locus_tag="VW157_000397"
     CDS             complement(30259..30453)
                     /locus_tag="VW157_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9551754.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            30619..31569
                     /gene="rsmH"
                     /locus_tag="VW157_000398"
     CDS             30619..31569
                     /gene="rsmH"
                     /locus_tag="VW157_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9551755.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVVRKIHK"
     gene            31581..31898
                     /gene="ftsL"
                     /locus_tag="VW157_000399"
     CDS             31581..31898
                     /gene="ftsL"
                     /locus_tag="VW157_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9551756.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            31902..34154
                     /gene="pbpX"
                     /locus_tag="VW157_000400"
     CDS             31902..34154
                     /gene="pbpX"
                     /locus_tag="VW157_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEU9551757.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            34156..35136
                     /locus_tag="VW157_000401"
     CDS             34156..35136
                     /locus_tag="VW157_000401"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9551758.1"
                     /translation="MFISLSAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            35745..37850
                     /locus_tag="VW157_000402"
     CDS             35745..37850
                     /locus_tag="VW157_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9551759.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEVEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(38147..38629)
                     /locus_tag="VW157_000403"
     CDS             complement(38147..38629)
                     /locus_tag="VW157_000403"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9551760.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(38724..40208)
                     /locus_tag="VW157_000404"
     CDS             complement(38724..40208)
                     /locus_tag="VW157_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9551761.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKSETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIKLLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            40361..41968
                     /locus_tag="VW157_000405"
     CDS             40361..41968
                     /locus_tag="VW157_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9551762.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLVVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            42126..42299
                     /locus_tag="VW157_000406"
     CDS             42126..42299
                     /locus_tag="VW157_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU9551763.1"
                     /translation="MNIKKRVLSAGLTFASALLLATCGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADNIVN"
     gene            complement(42292..42813)
                     /locus_tag="VW157_000407"
                     /pseudo
     CDS             complement(42292..42813)
                     /locus_tag="VW157_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            43036..44481
                     /gene="cps4A"
                     /locus_tag="VW157_000408"
     CDS             43036..44481
                     /gene="cps4A"
                     /locus_tag="VW157_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEU9551764.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLICFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYESKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIINSLHDSIQTNVPLETMINLVNAQLESGGNYKVNSQDLKGTGRTDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            44483..45214
                     /gene="cps4B"
                     /locus_tag="VW157_000409"
     CDS             44483..45214
                     /gene="cps4B"
                     /locus_tag="VW157_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEU9551765.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLVEAYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLEKKLIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QVNSSHVLKSKLFGERYKFMKKRAQYFLEQDLVHVIASDMHNLDGRPPHMAEAYDLVT
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            45223..45915
                     /locus_tag="VW157_000410"
     CDS             45223..45915
                     /locus_tag="VW157_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEU9551766.1"
                     /translation="MKEQNTIEIDVFQLFKTLWKRKLMILLVALVTGAGAFAYSAFIV
                     KPEYTSTTRIYVVNRDQGDKSGLTNQDLQAGSYLVKDYREIILSQNVLEKVATNLKLD
                     IPAKTLARKVQVTVPVDTRIVSISVKDKQPEEASRIANSLREVAAEKIIAVTRVSDVT
                     TLEEARPATTPSSPNVGRNSLFGFFGGAVVTVIAVLLIELFDIRVKRPEDVEDVLQIP
                     LLGVVPDLDKMK"
     gene            45925..46599
                     /locus_tag="VW157_000411"
     CDS             45925..46599
                     /locus_tag="VW157_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEU9551767.1"
                     /translation="MPTLEISQAKLDFVKKAEENYNALCTNLQLSGDDLKVFSITSVK
                     QGEGKSTTSTNIAWAFARAGYKTLLIDGDIRNSVMLGVFKARDKITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFSTMLETLRKYFDYIIVDTAPVGVVI
                     DAAIITQKCDASILVTKAGEINRRDIQKAKEQLEHTGKPFLGVVLNKFDTSVDKYGSY
                     GNYGKK"
     gene            46946..47545
                     /locus_tag="VW157_000412"
     CDS             46946..47545
                     /locus_tag="VW157_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9551768.1"
                     /translation="MYRVTKRLGDILLSLIGIIVLFPVFIVIAIAINTDSEGPIIFKQ
                     KRFGIYKEPFYILKFRSMKIGAPKNVAPRNLDNPEQWITKVGAFLRKTSLDELPQLWN
                     ILIGNMSIVGPRPAGINELDLLTEREKFGANDVLPGLTGWAQINGRDTLSIEMKAEAD
                     GYYVKHMSLSMDLRCIIRTIPYVLKRKGIVEGSGKKELD"
     gene            47550..48776
                     /locus_tag="VW157_000413"
     CDS             47550..48776
                     /locus_tag="VW157_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9551769.1"
                     /translation="MKILFVCQHYKPEPFRLSDICEDLVQRGHEVAVLTGIPNYPEGE
                     IYADYRKRKKRRETINGVAIFRSYTIARRQNTLYRILNYFSFALSSTIGVIFGRYKAK
                     DGSDFDCVFVNQLSPVMMAWAGIAYKNKYNKPMFLYCMDVWPDSLIVGGVKENGLIYK
                     IFEFVSKKVYQASDYIFVTSLSFKDYFVKKFNIPLHKITYLPQYAEDLFVPNELKTNK
                     NTIDLTFAGNIGKAQNLETILKAASAIEQIPDLAKRVHFHFVGDGTELLNMQKLACEL
                     ELENTSFYGRRPLEEMPDFYTKSDAMLVSLIGDSIISRTLPGKVQSYMAAGKPIIGAI
                     SGDTQRVVKEAKCGFISPEGNVDQLVRNIRKFCLLSVEEREKLGRQARCYYEEQFSKE
                     WFMTYLENHLKEGFLS"
     gene            48773..49942
                     /locus_tag="VW157_000414"
     CDS             48773..49942
                     /locus_tag="VW157_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9551770.1"
                     /translation="MKVLMINSVCGIRSTGRICADLATALTEEGYEVKIAYGREYVPK
                     QFQQYAVKIGTKSDIRFHAIRSRLFDESGFGSRQATVNFIKWMKEFDPDIIHLHNLHG
                     YYINVELLFDYLRICGKKIIWTLHDCWAFTGHSAYFDCLECESKGVCDHPSQKNDYPK
                     SFVNFSKRNYQRKKKCFTHIPNLTIVTPSQWLANLVRQSFLKEYPVKVIHNGIDTTVF
                     QPRLIAAQKLRKRHHLEGKQVLLGVAAIWDNRKGFSDMLALANDLTDNQRIVLIGLKD
                     RQLKELPKGIIGLTETNSPEELVAWYTLADVFLNPTYQDNYPTTNLEAISCGTPVISY
                     PTGGSVESAKLYGLVCQDRNVASILSSLEKVSQLSKTEKLDFSIASFIKQMKYLY"
BASE COUNT        15185 a         8883 c        11082 g        14795 t
ORIGIN      
        1 ccaactttta aaataggttt ctattagatt agtgggtgaa gtcgagtacc atacgtcctt
       61 ggatttggcc tttttccatt tcgtcgaaaa tggcaacagc atcttctact ggacgttttt
      121 gaacaactgg gactaccaga ccttctgcac caaattggaa ggcttcttct aagtctttac
      181 gagttccgac aagagaaccg atgacttgga ttccatcgag gactgtttta acgatgctga
      241 gttccatcat ttcagaagga agaccaacag cgacgacgcg accaccagca cgaatggagt
      301 caacagcctg gttgaaggca actttagaca cagcagttac gacagctgaa tgagctcctc
      361 catcagtttt ttctttaatg agtccagcta catcttcaac ttcgaggccg ttaatcacaa
      421 tgtcagcgcc tacttctttt gcaagggtaa gtttgtcatt gttgatatcg actgcgataa
      481 catgagcatt gaatactttt ttagcgtatt gaacagcgag gttaccaagt ccaccagcac
      541 cgtaaagaac aacccattgg cctggttcaa cttttgcttc tttgatagct ttataggttg
      601 ttactccagc acatgtgata gaagaagctt gggctggatc aagtccgtca ggaactttga
      661 cagcatagtc agcagttacg atacattgtt cagccatacc accgtctact gagtagccag
      721 catttttcac tgtacggcaa agggtttcgc gaccagttgt acagtattcg caagtgccac
      781 atccttcaaa gaaccaagca acgctgacgc ggtcaccgac tttaaggctt ttcacatctg
      841 gagcaatctc tttaacgata ccgatacctt cgtgcccaag aacacgtcct gggacttgac
      901 caaagtcacc atgagcaacg tggaggtcgg tgtggcaaac accacagtat tcaacttcta
      961 caagtgcttc cccagtttca agtggacgga gtactttttc ttcaatagca acaccagtgc
     1021 tttctggatt tacaacaaca gctttcatag ctatcctcct tgatattgac cgagaacagg
     1081 gacaacggga ctggattgat tttttgtcaa cagagtcgag tgtgacgagc tctcttgtat
     1141 ttatatgtga agtatatcac aaaagaaagc cttttccaag gttttggaga caaaaagtag
     1201 aacaatcgat taactggttg atgagactgt gaaaagagca caaagaccag cttgcaagct
     1261 ggtcatactc ttcgaaaatc tcttcaaacc acgtcagctt catctacaac ctcaaagcag
     1321 tgctttgagc aacctacggc tagcttccta gtttgctctt tgattctcat tgagtatcag
     1381 tataggttat aagaataagt cttgtagggt tttggcaacc ccatcttggt cgttggtaag
     1441 ggaaatttgc tcatctgcat aagggagtag ctctggattg gcatttttca tggcataacc
     1501 cttcccagca aaagcgagca tttcggtatc attgtgttca tctccaaagg caatcaaatc
     1561 ttttttgtca cgattcatta tcttgagcaa gtagtccaaa gcaaaggcct tgttgacacc
     1621 ttttggggta cattcaagga tattgagcgg acctccccag gtattgatag aaagttgatg
     1681 ctggtagaag gcgttcattt cttttgccaa ggcatatttg tcactggctc tggtctgcaa
     1741 gaggatacag ttagggtcct tggtcaccaa ttcaggctgg aattgatctt caggctggaa
     1801 agcttctaca ccaaatagtt tgggattggc aatttcttca ttgggatttg taatgtagaa
     1861 ttttttacga tattctccag cgataaaatc ggcttgaatg tcctctgaac gttgaaccat
     1921 atctagcaga tattttttgt ctacagtcaa acacttttca aaatcccaaa cttggtctgg
     1981 taaatgagta agggatccgt tgaagttaat cataggagtg tctaagccca gttcacggta
     2041 aaaatctttt gacatacggt aagggcgacc tgtcgtaata ataacctgat ggcctttttc
     2101 agcaactttt ttgatggttt ttttggtaaa gtcagaaatt tgactgtctg agttgagcag
     2161 ggttccatct aggtcaactg caataatttt tttcgtcata tggacctttc tagtgtgttt
     2221 tcagataaga tgtcttctat tataacagaa agcaggcaga aaagcagatt ccaaacaaaa
     2281 aatgaaattg gattaatttc ttaaacaaat caaacaaaga cattgaaaaa gtgaatgata
     2341 aatgatataa ttctattatt gttcgtaaaa attaaaagga gattgatgat ggacaaatta
     2401 tttaaactaa aagagaacgg tacagacgtt cgtacagagg ttctcgctgg tttaacaact
     2461 ttctttgcaa tgagctatat tctctttgta aacccacaaa ttctttcgca aacaggaatg
     2521 cctgctcagg gtgtgtttct tgcaacgatt atcggtgcag ttgctggtac cttgatgatg
     2581 gccttctatg ctaacttgcc atacgctcaa gcaccaggta tgggactcaa tgccttcttt
     2641 acctttacag tcgtatttgg acttggttat tcatggcaag aagccctagc tatggtcttc
     2701 atctgtggga ttatttcatt ggttattacc ttgacaaatg ttcgtaaaat gatcattgaa
     2761 tcgattccaa atgcccttcg ctcggctatt tcagctggta tcggtgtctt ccttgcctat
     2821 gtagggatta agaatgctgg acttttgaaa tttacgattg atccaggcaa ctatactgtt
     2881 gtaggagaag gggctgacaa agctcaagca acgattgcag caaactcttc agcagttcca
     2941 ggcttggtca gctttaataa tccagctgtt ttagtggctc ttgcaggact tgccattact
     3001 atcttctttg tcatcaaagg gattaaaggg ggaattattc tctctatctt gacaacaact
     3061 gttcttgcta ttgcagttgg tttggttgat ttgtctagta tcgattttgc taataaccat
     3121 gttggtgcag cttttgaaga tttgaagaca atctttggtg caactcttgg ttcagaaggt
     3181 ttgggagctt tggtttcaga tacagctcgc ttgcctgaaa ctctgatggc cattcttgcc
     3241 ttctcattga cagatatttt tgacacaatt ggtaccttga tcggtacagg tgaaaaagtt
     3301 ggtattgtag cgacaaatgg tgaaaatcac caatcagcca aattggataa ggctctttac
     3361 tctgatttga ttggaacgac agtcggtgcc attgcaggta cttcaaacgt aacgacttat
     3421 gttgagtctg ctgctggtat cggtgcaggt ggacgtactg gtttgacagc cttggttgta
     3481 gctatctgtt ttgcgatttc aagcttcttt agcccacttc tagcgatcgt accaacagcg
     3541 gctacagctc caatcttgat tatcgttggg attatgatgc ttggtagctt gaaaaatatc
     3601 cattgggatg atatgtctga agcagttcct gccttcttca catctatctt tatgggattc
     3661 agctactcta tcactcaagg gattgcagtt ggtttcttga cttacacttt gactaagctt
     3721 gtcaaaggtc aagttaaaga tgttcatgcc atgatttgga ttttggatgc cttgtttatc
     3781 cttaactaca tcagcatggc cttataatag aatgacccag ggggatttcc cccctttttt
     3841 aatacaaagg agataggtga tgaaagagaa aaatatgtgg aaagaattgt tgaatcgtgc
     3901 aggctggatt ttggtctttt tacttgccgt ccttttatat caggttcccc tagtggttac
     3961 ctctattttg actttaaaag aagtagccct gctacagtca gggctgatag ttactggcct
     4021 ttcaattgtg gttctggctc tatttattat gggagctcgt aaaaccaagt tagctagttt
     4081 taatttttct ttttttagag ctaaagattt ggcacgtttg ggcttgagtt atttagttat
     4141 tgtcgggtca aatatacttg gttctatctt gttgcaactg tcaaatgaga cgacaacagc
     4201 taaccagtct cagattaacg atatggttca gaatagttct ctgatttcta gcttcttctt
     4261 gctagccttg cttgctccaa tttgtgagga aatcttgtgc cgtgggattg ttcctaaaaa
     4321 gattttccga ggcaaggaga acttgggatt tgtagtcggt acgattgtgt ttgctttatt
     4381 gcatcaacca agtaatttac cttctttatt gatttatgga ggtatgtcga cagttctatc
     4441 ttggacggcc tacaagaccc aacgtttgga aatgtcgatc ttgcttcaca tgattgttaa
     4501 tgggattgct ttctgtttgt tggctcttgt ggtgattatg agtcggacat taggaatttc
     4561 tgtttaaaag tttttatgta ggaaccgacc tctttctact agggaaagat gaatgcaatc
     4621 gtgtccatct ttttcttttt atggtaaaat agaaaaataa tatgatgaaa atccttgagg
     4681 gagtgaccga tatgtcaagt aaagccaatc atgcaaagac agttatttgc ggaattatca
     4741 atgtaacccc agactccttt tcggacggtg gtcaattttt tgctcttgag caggcactcc
     4801 agcaggctcg taaattgata gcagaaggag ccagtatgct cgatatcggc ggagaatcga
     4861 ctcggccggg aagtagctat gttgagatag aagaggaaat ccagcgtgtt gttccagtga
     4921 tcaaagcgat tcgcaaggaa agtgatgtcc tcatctctat tgatacttgg aagagtcaag
     4981 tagcagaggc tgctttggct gctggtgccg atctagtcaa tgatatcact ggtctcatgg
     5041 gtgatgaaaa aatggcccat gtggttgcta aagctggagc gaaagtggtc atcatgttta
     5101 acccagttat ggctcgacct cagcatccta gttcgcttat cttccctcat tttggctttg
     5161 gtcaaacctt tacagaaaaa gagttagctg actttgaaac attgccaatc gaagacttga
     5221 tggtggcttt ctttgaacga gcactagcga gagcggcaga agctggtatt gcaccagaaa
     5281 atatcctgtt ggatccagga attggctttg gtctgaccaa gaaagaaaat ctgcttcttt
     5341 tacgggacct ggataaacta catcagaagg gctatccaat ctttctcgga gtgtcgcgca
     5401 agcgatttgt catcaatatc ctagaggaga atggttttga agtcaatcct gagacagagc
     5461 ttggtttccg caatcgggac acggcttcgg ctcatgtaac cagtatcgct gcaagacagg
     5521 gtgtagaagt ggtgcgcgtg catgacgtag ctagtcacag gatggcagtt gaaattgcct
     5581 ctgccattcg tctggctgat gaagcggaaa atttagattt aaaacaatat aaataagatg
     5641 aaagaaattg aaaacaatca gtggattgct aactaccgga cggatcaacc gcattttggc
     5701 ttggaacgaa tggtggaact gttagctttg cgtggcaatc cccatctcaa actcaaggtc
     5761 ctccatatcg gaggaactaa tggcaagggc tcgactattg cttttttgaa aaagatgcta
     5821 gaaaagctag ggctgagagt tggcgtgttt agctcgccct atctcattca ttacacagac
     5881 cagattagca tcaatgggga atcgatctca gaagcgaggc tagaagctct catggcagac
     5941 tatcagtctt tgctggaggg agaagcggtc gccaatttac agggcacaac cgagtttgag
     6001 attatcacag ccctggccta tgactacttt gcctcagagc aagtagatgt ggccatcatg
     6061 gaagttggca tgggtggact ttttgatagt accaatgtct gtcagcccat tttgacagga
     6121 attacaacta ttggcttgga tcatgtggcc ttacttggtg acaccttgga ggccatagca
     6181 gagcagaagg caggtattat caaacaaggg atgcccttgg taacagggcg tattgctcca
     6241 gaagccttgg ctgtgattga ccgcattgcg gaagggaaag atgcgccgag acttgcctac
     6301 gggacagatt atcaggttcg tcatcaagaa agtgtggtga caggggaagt ctttgactat
     6361 acaagtgctg tcagacaagg tcgcttccag actagcctgc ttggtttgta ccaaatagag
     6421 aatgctggga tggccatagc tttacttgat actttttgtc aagaagatgg tcgagagcta
     6481 gcaagcaatg actttcttgg tcaagccttg gaagaaacaa gttggccagg gcgtttggaa
     6541 atcgtgtcaa gagatccctt gatgattttg gatggagccc ataatcccca tgctatcaag
     6601 gccttgttgg taaccttgca agaacgtttt gcggattatc ataaggaaat cctcttcact
     6661 tgtatcaaaa ccaaagcctt ggaggatatg ttggacttgc tgggagccat gccagatacc
     6721 gagcttactc taacacattt tgcggatagt cgggcgacgg atgaaagcgt gctgaaagag
     6781 gcagctaagt ctagaaatct cagctaccaa gattggcatg attttctaga gcagaatgtg
     6841 acagataaaa aagaagagaa acaaacagtt aggattgtca caggttcctt gtatttcttg
     6901 agccaagtga gggcctatct gatggagagg aagaacgaga atggatacac aaaagattga
     6961 agcagctgta aaaatgatta tcgaggctgt aggagagaac gctaatcgcg agggcttgca
     7021 ggaaacacct gctcgtgtag cccgtatgta tcaagagatt ttttcaggtc ttggtcaaac
     7081 agcagaggaa catttgtcaa aatcctttga aattattgac gataatatgg tggtagaaaa
     7141 ggatatcttt ttccatacca tgtgtgaaca ccacttcttg ccattttatg gtagagcgca
     7201 cattgcctac attccagatg gtcgtgtggc aggcttgtct aagctagccc gtacggttga
     7261 agtttattcg aaaaaaccac aaattcaaga acgtttgaat atcgaagtgg ccgatgcctt
     7321 gatggactat ctaggtgcta aaggagcctt tgttgtcatt gaggcggaac atatgtgtat
     7381 gagtatgcgt ggtgttagaa aaccaggcac tgcaaccttg acgacagtag ctcgtggtct
     7441 atttgaaaca gataaggacc tccgcgacca agcttatcgt ttaatggggc tataaaaaga
     7501 atccgtttca agcggatttt tctagaaagg aatcattatg gatcaactgc agattaagga
     7561 tttggaaatg tttgcctatc atggtctttt tcctagtgag aaagaattgg ggcagaaatt
     7621 tgtcgtttca gccatcctat cctatgatat gaccaaggca gctacagact tggatttaac
     7681 agcctctgtc cattacggag aattgtgtca gcagtggacg acttggtttc aggaaacgag
     7741 tgaagatttg attgaaacgg tagcctataa actggtggaa cgtacctttg agtcttatcc
     7801 tcttgtccaa gaaatgaagt tggaactgaa aaaaccttgg gcaccggtgc atttgtcact
     7861 agatacttgc tcggtaacca ttcatcgccg caagcaacga gcctttatcg ccctaggaag
     7921 caatatggga gataaacaag caaacttgaa gcaagccatt gacaaactgc gagctcgtgg
     7981 catctatatt ctcaaagagt ccagtgtctt agcgacggag ccttggggtg gagtggagca
     8041 ggatagcttt gccaatcaag tggttgaggt ggaaacctgg ctaccagcac aagacttgtt
     8101 agaaaccttg ttagccattg agtcagagct gggacgggtg agagaagtgc attggggacc
     8161 tcgtttgatt gatttggact tgctctttgt ggaggaccag atcctttata cagacgacct
     8221 catattgcct catccttaca tagcggaacg cctttttgtc cttgagtcct tacaggaaat
     8281 tgcgcctcat tttatccatc cgatattaaa acaaccgatc cgtaacttgt atgatgcttt
     8341 gaaaaaatag aaaaactcta gttttcagtt acttgcaact gaaggctaga gtttttaaaa
     8401 taggtcattt tcttctggga ggaggatagt ttctctaccg tccatgtcta aaaccagtac
     8461 tcttggggga taacgagggt cgaaaggatg gttaaagtca aaatcaatgg ctgtagggag
     8521 gtgttgactt gaaaagtgga aggtaatctt tccttggtta ttaagcaatt gaaactcgag
     8581 ttcttcttcc aattcaaaga cattttttaa gaaatggtcg ataatatacc aaaaagagtc
     8641 aatgatgtca tcaggcaagc tggtaacaat gccaaaacta gcagaccgca tgtgggtatt
     8701 ggtaaaagcc atatctctgt ctcctttctt ttcccttatc atacagcaaa taggattaaa
     8761 aatcaagaaa aggtgatttt ttgaaaagga ttttaactac agggaaaaag aaggaaaaaa
     8821 attcccaaaa atctaccgaa gttaataggt aaattcccaa attaacttga ttatataact
     8881 ttcagttact ttgagaagtt accgaaaaat atttttcata tctattgact tttaggggtg
     8941 aaatttggta tgatagtagg cggtattgtt taccccattt gaaaggcccc ggaaccttcc
     9001 aaatactttt cgatgggaag gaacacccat caccgtaaac aaaaatcgaa ctatatatag
     9061 gagaaatcat gaacaaaaca acatttatgg ctaaaccagg ccaagttgaa cgtaaatggt
     9121 acgtagttga cgcaactgat gtaccacttg gacgtctttc tgcagtagtt gctagcgtac
     9181 ttcgcggaaa aaacaaacca acatttacac cacacactga tacaggtgac ttcgtgattg
     9241 ttatcaatgc tgaaaaagtt aaattgactg gtaaaaaagc aactgataaa atctactaca
     9301 ctcactcaaa ccacccaggt ggattgaaac aaatctctgc aggtgaactt cgttctaaaa
     9361 atgcagtacg tttgattgag aaatcagtta aaggtatgct tccacacaat acacttggac
     9421 gcgctcaagg tatgaagttg aaagtatttg ttggagctga gcacactcat gctgcacaac
     9481 aaccagaagt tcttgacatt tcaggactta tctaaggaaa ggaacaaata aagtatgtca
     9541 caagcacaat atgcaggtac tggacgtcgt aaaaacgctg ttgcacgcgt tcgccttgtt
     9601 ccaggaactg gtaaaatcac tgttaacaaa aaagatgttg aagagtacat cccacacgct
     9661 gaccttcgtc ttgtcatcaa ccaaccattc gcagttactt caactgtagg ttcatacgac
     9721 gttttcgtta acgttgtagg tggtggatac gctggtcaat caggagctat ccgtcacggt
     9781 atcgctcgtg cccttcttca agtagaccca gacttccgcg attcattgaa acgcgcagga
     9841 cttcttacac gtgactcacg taaagttgaa cgtaagaaac caggtcttaa gaaagctcgt
     9901 aaagcatcac aatttagtaa acgttaattc gaaagaatta ctatacttat acagagcacc
     9961 tttcggggtg ttcttttttt atactttctt actaaattgg tgcaattgac acagttgttg
    10021 cgactttagt cgcttacaaa tgtggctgca acctgacaag gtcagttgcc tcaaaacgtt
    10081 aatcaataca attatatcaa cgtttcaaag cactcaagag tttacctcat aggtgctttt
    10141 tttatgtttt ttgaaaaagt tcacctcaaa gtttacctta ttttcacctt attgttttag
    10201 agactgtaag gcaaattcac cgacggccat agtagggact acattagaag tattgacata
    10261 aatctgtgtt gtacctgtgt cgctatgtat tagagcttca gaaatggctt ctaagctcat
    10321 tcctgcttgt ttagcgagcg ttgctccggt atgacgtagt ttgtgtggcg tagcgtgtgt
    10381 aagctctggg tgtcgttttc tgatagtttt catcttatta ttgagatagt cagaatgtaa
    10441 aggtttattg atattgcctt tggtatctat gtaagtaaag atgaattgtt ctggattact
    10501 gatgatacca aactttgcca gttcatattt ttgttgtttt ttccatgaag tcagaagttg
    10561 aagtaagtca ttagaaatag agaaaatggg aaatgtttcc acaataaaac gcatattatc
    10621 agatggttaa tgcgttttat atttgggttt gaggcttata aaataggata agcgtactga
    10681 aaacttagat atgacaaaca aaataaaatt tttaaaagct acagagaaac atggtataat
    10741 gtatctaaag gaggtagcat atgtccttag atatagataa agaaaagatg acaatcatgg
    10801 gaatagcttt tgaaaaccga tccgttttta agagtgtttg gtatgcttta agtacaaata
    10861 tgattgaagg atggcgacct acggttagtg atgttgaaaa attacgagat gaagctcttg
    10921 ctctggggat gacttaatga agcgcaactg ttatgagtct tatgactatg ttgatccgga
    10981 taatttatat accgatccgg gttcttcagt tctaagaaat aagcaggaag gaaaaaagca
    11041 cgcgagcttg aatatcgtat gattgctagt aagagtttga aattatttat caatcctatc
    11101 ttagttaata gtttaaaagt agtttgtttc acaactgcat aagcttagaa aagttgaaaa
    11161 gtgataactc tttaataaac tcttggtttg ttaaaagata ttttgtccac tacatttttt
    11221 caattgacta ttatttgtca tagatataac agatagaaca ttgcattcag aataatgaaa
    11281 aacgcatcaa aaatggtgcg ttctgttttt atgctaccat aaaaggagga aaacaaattg
    11341 aaaattttta agggagagtt ttatcgaatc tctgtattaa cagacaagct agtaaggtta
    11401 gaatactctc aaactggaag ttttgaggat agaacgacac aacttatcta taatagagat
    11461 tttggccaag tttcgttaga ttatatcgag acatcaaacg tactagatat tatgacggac
    11521 tattttcatc tgcactttaa taaaggagaa tttaacgccg aaaatttatt tatagaatta
    11581 aaaggaaatt ttgccgtata tggtagtcgc tggtattttg gtgaatctat tgaaacgtta
    11641 aaaggaacag ctcggactct ggataaggca gatggagcaa tctcgttaga agatggaatt
    11701 attagccgaa atggtatagc cttattggat gattctcaag gatttatttg ggatgaacaa
    11761 tctggttata ttgagagaga aaatcaaatt gacctgtatt tctttgccta tgggcatgat
    11821 tatagaggag caatcagaga cttttaccat ttgactggtt caacaccctt gttgccaaga
    11881 tatgctttag gcaattggtg gagtagatat tggccttata cgtcggatga atacttggat
    11941 ttaatagaca gatttgcaac agagaaaatt ccattatcta tcggtgtgtt agatatggat
    12001 tggcatataa ctgacattcc agctcgtttt ggaagtggct ggacaggata tagttggaat
    12061 agaaacttaa taccaaatcc agaacagtta ttgcaacaac ttcatgatag aaagttaaaa
    12121 ctctccttaa atgtccatcc tgctgatggg atacgggctt atgaagaagc ttatcctcaa
    12181 atcgcaaaac ggttggggtt aaatgtagaa ctagaagaac ctgctatttt tgattttttt
    12241 aatccctctt ttagggaagc ctactttaaa gatgttcatt atgagctaga aaagcaggga
    12301 gtagattttt ggtggattga ctggcaacag gggacacaag gcatgctaga tccactttgg
    12361 cttttaaacc attatcacta tcaggatagt tgtaaaaatg cagaaggtgg tttgatttta
    12421 tcaagatatg caggtcctgg tagtcaccgc taccctgttg gtttttcagg ggatactatt
    12481 atcagttgga attccttaag atttcaaccc tattttacag cgacagcatc taatatcggt
    12541 tatagttggt ggagtcatga tatcggtgga catatgttgg gggattatga cgaagagcta
    12601 caaactagat ggctacagtt tggtgttttt agtccgatta ctcgattaca tagttctaga
    12661 agtcctttta atagtaaaga accttggttt ttttcagaaa caacatctaa gattatgaag
    12721 aaataccttc gtttgagaca tcagatgatt ccttatctat ataccatgaa tgtaaagaca
    12781 catgaggaag gtgccccatt aatcagtcca atctattatt tctatccaga gaatgatgag
    12841 agctataatg ttccaaacca atactttttt ggaacagaac tgatggtggc tcccattgta
    12901 gaaaagatgg atttgacatt ccaatctgca aaagtagatg tatggttccc tgaaggtgaa
    12961 tggtatgact tcttttcaga gaaaaaatac acaggtggtg tgaagttaag tgtttatagg
    13021 gacatctcga ctacgcctgt gtttgcaaaa agtggtgcaa tcattccctt ggttggttct
    13081 gagataggta tgggtgttga tttacctgag gttgtagatt ggtatgtatt cccagggaaa
    13141 caacattctt ttgaaatgct tgaagatcaa aatggtcaaa gatataaaac aagattatca
    13201 atcgattggg aaatgggaat ggtagagtta gcattacaag gagattctag tatcgttcca
    13261 agcaatagaa aacatagaat tcattttaaa ggaacgaatg tgtctataat tgaattgcca
    13321 aataagaatg atacagctaa atttgaatgg aaagataata aaaggacatc tttaaatgac
    13381 gaagttttta gactactaaa gacagcttct cttccatatg aattaaaaga tagattgtta
    13441 aatcaattca tcaatgcgaa aaactctcat gacttaatga atatcttgca tcatcaggat
    13501 aaggaattga gagggcgttt gttggaaatg atatttacta gccaaaacta attgaaataa
    13561 ttagaaagtc atttgtttag tgtatatgaa gattctgtat ggtattcgtt atcactggtc
    13621 acattgaaaa tgtgaaataa gcacctacaa catagacttt acaacacttc atgtttcata
    13681 tcatggggtg tttttctatt taagtcaaag cccatcaaaa aatctcacta ttcatcaggt
    13741 taaaggaaac ctaatcatag tgagattgtg aattatagta gagtttgtat cgcctcttca
    13801 atttgttttg ggtctgtttt tgaagaaaag cgttcaaaga cttgcccatc tcgaccgatg
    13861 agaaacttag cgaaattcca ttcgattcgt tttcctagtg ggccggattt ctggtctttt
    13921 aaccagacat agagagggtc tgcttcctta ccgttgacct taatcttggc aaaacgtggg
    13981 aaggtggttt gaaaatgtag gctacagaag gcgttgattt cctctgcgct gccgggtgct
    14041 tgtcccataa actgattgca agggaaatcc aatatttcga agccctgatc ttgatagcgt
    14101 tcatagagtt cttgaagtcc ctggtactgg ggcgttaaac cacatccagt agcagtgttg
    14161 acaatcaaga gaactttgcc acgatagcta tccaagggag ttgcttggtt gttttggttc
    14221 aaaacggaaa aatcataaag tgaagtcatg agggcctctt ttctttttct acattgtaac
    14281 agtttttact tttttcgtcg agtcggatgg aagatattgt tgaagaataa gccttcaata
    14341 tgatagaaac cgtttacaaa taactttaaa aatggtatga tttaattaaa ccggtttact
    14401 tagttgctat aaatctctaa tttgagattt agttcctatt gattttacaa tatgtttatt
    14461 ggagtgtata catgcaaaca aaaacaaaga agctcattgt gagtttgtct tcacttgttt
    14521 tatcaggatt tttattaaac cattatatga cagttggagc ggaagaaacg actacgaata
    14581 ccattcagca aagccagaag gaagttcagt atcagcaaag ggatacaaaa aatttagttg
    14641 aaaatggtga ttttggtcag acggaggacg gaagcagtcc gtggacagga agcaaagctc
    14701 aggggtggtc agcttgggta gaccagaaga atagttctgc agatgcctca actcgagtca
    14761 ttgaggctaa ggatggggct atcactatct caagccctga gaaattaagg gcagcggttc
    14821 accgtatggt ttctattgaa gctaagaaaa agtataaact gcgtttcaag attaaaacag
    14881 atcataaagt cgggattgcc aaagttcgta tcattgagga aagtgataag gacaagcgat
    14941 tgtggaattc tgcaacgacg tcaggaacaa aggactggca gaccattgaa gcagactata
    15001 gcccgacttt agatgttgat aaaatcaagc tggagttatt ctatgaaaca ggaactggga
    15061 ctgtttcctt taaggatatt gagctggtag aggtagcaga ccagccttct gaggattctc
    15121 aaacagataa acagcttgag gaaaagattg atttaccaat tggaaaaaaa catgtttttt
    15181 ctcttgcgga ctatacttat aaggtagaaa atcctgacgt tgcttcagtc aaaaatggaa
    15241 ttttagaacc tcttaaggaa gggacaacca atgtcattgt cagtaaagat ggcaaggaag
    15301 tgaaaaagat tcctttgaag attctagcct ctgttaagga tacatacaca gaccgtttgg
    15361 atgactggaa tggcatcatc gctggaaatc aatactatga ttctaaaaat gaacagatgg
    15421 ccaaattaaa ccaggaattg gaaggaaagg tagctgatag cctatccagt atttcaagtc
    15481 aggcggaccg cacctatttg tgggaaaaat tttcaaatta taaaacgtct gcaaatctga
    15541 ctgccactta tcggaaattg gaggagatgg ccaagcaagt gaccaatcct tcttctcgtt
    15601 attatcaaga tgaaactgtc gttcgaacag tcagggattc catggaatgg atgcataaac
    15661 atgtctacaa tagtgaaaag agcattgttg ggaactggtg ggattatgaa atcggtacac
    15721 ctcgtgccat caacaatacc ttgtctctga tgaaagaata cttctctgat gaggaaatta
    15781 aaaaatatac agatgtgatt gaaaaatttg taccagatcc cgaacatttc cgaaagacga
    15841 ctgataaccc attcaaggct ctaggtggaa acttagttga tatgggaagg gtaaaagtaa
    15901 tagctggttt actgcgtaag gatgatcaag aaatttcttc taccattcgc tcgattgagc
    15961 aagtgttcaa gttggtagac caaggtgaag gtttttatca agatggatcc tatatcgacc
    16021 acaccaatgt tgcctatacg ggtgcttatg ggaatgtttt gattgatggc ctgtctcaac
    16081 tgttgccagt cattcaaaag accaagaatc caatcgataa agataaaatg caaaccatgt
    16141 accactggat tgataaatcg tttgctcctt tgctggtgaa tggagagctg atggatatga
    16201 gtcgtggacg ctcgatcagt cgtgcaaata gcgaggggca cgtggccgca gtagaagtac
    16261 taagagggat tcaccgaata gcggatatgt ctgaaggaga aaccaaacaa cgtttgcaga
    16321 gtcttgtgaa gaccattgtt caatcggata gttattatga tgtctttaag aatttgaaga
    16381 cttataagga tatcagtttg atgcaatcct tgttaagtga tgcaggagtc gcaagtgttc
    16441 caagaacaag ttacctatct gcctttaaca agatggataa aacagccatg tacaatgcag
    16501 agaaagggtt tggatttggc ttgtcactct tttccagtcg taccttgaat tacgaacaca
    16561 tgaacaagga aaataaacgt ggttggtata cgagtgatgg gatgttctat ctttacaatg
    16621 gcgatttgag tcactatagc gatggctact ggccaacagt taatccatat aagatgcctg
    16681 gtacaacaga gacggatgct aagagagcgg atagcgatac aggtaaagtt ctaccgtctg
    16741 ctttcgttgg aacgagcaaa ctagatgatg ccaatgcgac agcaaccatg gatttcacca
    16801 actggaatca aacattgact gctcataaga gctggtttat gctgaaggat aagatcgcct
    16861 ttttaggaag caatatccaa aacacttcaa cagatactgc tgcaactaca atcgaccaga
    16921 gaaaactgga atcaagtaat ccatataaag tctatgtcaa tgataaagaa gcctccctta
    16981 cagaacaaga aaaggattat cctgaaaccc aaagtgtctt tttagaatcg tccgattcga
    17041 aaaagaatat tggttacttt ttctttaaga agagttcaat cagtgtgagt aaggctttgc
    17101 aaaagggagc ctggaaggat atcaatgaag gacagtcaga caaggaagtt gaaaatgaat
    17161 ttcttacgat tagtcaggct cataagcaaa atggagattc ttatggctat atgctcatcc
    17221 ctaacgtggg tcgtgccacc ttcaatcaaa tgataaaaga gttagaaagt agcctcatcg
    17281 acaataacga aacccttcag tctgtttatg atgccaaaca aggagtttgg ggcattgtga
    17341 aatatgatga ttctgtctct gctatttcca accaattcca agttttgaaa cgtggagtct
    17401 ataccattcg aaaagaaggg gatgaatata agattgccta ctataatcct gaaacccagg
    17461 aatcagctcc agatcaggaa gtctttaaaa agctagagca agcagctcag ccacaagtac
    17521 agaattcaaa agaaaaggaa aaatctgaag aggaaaagaa ccattcggat caaaagaatc
    17581 tccctcagac aggagaaggt cagtcaatct tggcaagtct agggttcttg ctacttgggg
    17641 cgttttatct attccgtaga ggaaagaaca actaatttgt tcatcatcta gatgaattac
    17701 atgaaactgt ggaaaataga aaaaatccga agaaggccaa atatcttcgg atttttcttt
    17761 taaaaaagat aagttgatac ttatcctttt ttacttcatt atgtgactat agcgttttga
    17821 aactagaata ggacgttata actactaaaa gatttctata aattcatttg attttcctaa
    17881 tcaatttgtt cgtatcctat ttcactccac tatattagtc cgtctcgctc cgtgaggtgc
    17941 gagacaaaac aaccacccgc tatgcgggtg cgcgtcgaag gttataccaa aaaaactcca
    18001 aacgcgatac aataaaggtg ttcactccac ttgtaaagcg aaaggagaaa aaatatggca
    18061 caaaaggctc atagtttatc tcacacaaag tggaggtgtt ctatcacatt gtgttcaccc
    18121 ctaagtatag acgaaaagtc atctataatc aatatagaag tagtttaggc gaaatatttc
    18181 atcgcttgtg tagttataaa ggtgttgaaa tgatcgaggg tcacttaatg ccagaccatg
    18241 tacacatgtt agtcagtatt ccgtcgaggc taagtgtttc aagttttatg gggtatttaa
    18301 aaggcaaaag tgcactcatg atgtttgaca aacacgccaa tctcaagtac aagtttggga
    18361 atcggaattt ctgggcggaa ggttattatg taagtccagt agggcttaat gaagccacaa
    18421 ttaagaaata tagtcaagat taaagaaatt atcgagtgga tgatttcttc acgagtatga
    18481 aaatttgaga actagtaaag tatgatatat agtaaaatga aataagaaca ggacaaaatc
    18541 gatcaggaca gtcaaatcga tttttaacaa tgttttagaa gtagaggtat actattccag
    18601 tttcaatcta ctatagcact agataaattg agtgtaaaag aatatgagga tccctttagg
    18661 gatagtggta agtaatacca aagtctctta aagaggcaag tgacgagtca agagcaataa
    18721 ggcttgaaca acgtgaaagc cagcgtcttt aggcgctggc tgatgatttg ggcttatagc
    18781 tctgagataa accacccgtt agacgggtag ttatgatttt atctgagtgt aatatactgt
    18841 tgggcaatct cgctgatgcg gtcaaagttg ccttgggaag cgagtttatt gagttcgcca
    18901 ccaattccaa cggcatctgc accagcagcg aaccattgag ggatgttgtt taggccgact
    18961 cctccggtta ccattacgga aacttgtggg atcggtgcct tgactgcaga gatatatgct
    19021 ggactgagag tactacctgg gaagagtttg atgatttcac taccggcttc aagtgcagtc
    19081 gtgatctctg tgagggtaat acagcctgga atgtacggtg tgctgtagag attgcacatt
    19141 ttcgcagttt cagcatggaa agatggagaa acaacgtaat ttgctccagc tagaatggca
    19201 tctctagcag ttaaggcatc aagcacagta cctgcaccga tacaaacact ctgatcgtcc
    19261 tgatacaagt ctacaagttc cttgatgatt tgtcctgcat actgattggt ataggcgatt
    19321 tcaatagctt tgataccgcc cttgatacaa gcaatcgagg cttgtagtcc ttcttccttt
    19381 gtatttcctc gaatgacagc gacaattttc tgttttttta gttcaataat cgtatctgat
    19441 ttggtcatgt aattctccta acgaatgata tcttgtgcat ttgccagtaa attttcaata
    19501 ctagttgcgg aagtggagag atggtctcct ggaagagtgc atttgagagt tgcgctcgca
    19561 actgcaaaat caatggtagt ttttagggag gaatgatgga gtagttggta aagggcacca
    19621 gatataaagg catccccgct accaattcgt tgatagactg cagtttttag ttggacagac
    19681 tcttcaaatc gttcttccag agcataggct tgatagacat ttttgtcttg ctcatcacta
    19741 gatcggaggg tatggaaaat ggccttgaaa ccataggctt cttttaaaag tcgcatgcga
    19801 ttttccacct cttctaggct agcactgtct cttggaaaca tctctagatt ttggtcatca
    19861 atcatgagag gatcaatacc gaagcaatag tcagtaaaac gtgcaaactt agaaaattca
    19921 tacttggctt ctaggactga aatcatcttt gttctcagat tgagatccat tgaaacgaca
    19981 attcctcggc gcttggcttc ttccaagagt aggagaagga tcgcacggac ctcttgaccg
    20041 atagctatgg tgattccact aaaatgaaaa tggctaatcc cctgaaagag agaatccata
    20101 tctagcatgt ttggccgaat ctggctgata ctcgtatgct tacgatcgta gaaaacttca
    20161 ctttgacgac aaccaaagcc gttctccaaa tagtagaggc cgattcgatc gccaagccga
    20221 cagattgaac tggtatcgat ttggtgctgt ttcaagaatg tgagaaaacg atctccaatc
    20281 tcgttggcag ggagtgcggt aaagactttc gttgagatac ccagggcttg caaattacaa
    20341 gcgatgttaa tttctgatcc gccaaaataa gtcgaactgg caacatgatc gccgatactg
    20401 gtggcatcta atggtgagat tcgaattaat ggttcgccaa acagtaatat tttcgtcatc
    20461 cattagttcc tttctggttt agaatagctt tacttggcta atacttcttt caaataagca
    20521 gcgacttcat catcttggca gttttcaaag aagtattctt ggaagtattt gccagagatg
    20581 gtgtctttga ggaagtcttg gtcgattgtt ttaaggatgg tcatcaaatc attgtgggtg
    20641 atttgtttca cctcgtttaa gatacgagcg ttacgttgtt cagggattac acgttctctt
    20701 gggtagccac cgcccatttc ttcagcaaat aagcgttcaa acatcaattt gagggtcagt
    20761 tctgcccccc agccaaatcc tttggcataa gggatagaca aggcgttacc accattgatt
    20821 tgagaataaa ggtaagcgtc agttgggtcc actgctagac cacagacaac accagggaag
    20881 ctgtttaaag caagcatagc ccctacaccc gtaccacagc cggtaacaac aaagtcaact
    20941 gcctttgtat ttaaaaggat ggcagccatt agtccgttct gcacataagt taattgactt
    21001 tctccttctt ctccacgcat accatagtta aataattggt agtctttttt atctgtcgct
    21061 tcttttagac tatcgtaaat aatgtgattc ttgctagctt gactattttc attgattaat
    21121 gcgattttca taagatcttc tttctatttt tactcaggtt gttttccgat gtaggctaag
    21181 ataccgccat ctacatatag gatgtggcca ttgacaaaat tgctggcatc actagcgaga
    21241 aagacagcag ggcccatcaa atcttcagga tttccccaac gtgcagcagg tgtttttgca
    21301 atgatgaact ggtcaaatgg gtggcgagaa ccatcttctt gcaactcacg aagaggtgct
    21361 gtttgaggag tggcaatata acccggtcca attccgttac attggatatt ggctccaccg
    21421 tattcagacg caatgttgcg ggtcaacatt ttcaagcccc ctttagcagc agcataagcg
    21481 ctaactgttt cacgtcccag ttcgctcatc atcgaacaaa tattgataat ctttccatgc
    21541 cctttcttta tcatagaagg aataactgcc tttgaaacga taaatggtgc gtttaagtca
    21601 atatcgatga ccttacggaa atcagcggcg ctcatttcgc acattggaac tcggcggata
    21661 ataccagcgt tattaacgag gatgtcaatg acaccaacct cttgttcgat ttgcttgacc
    21721 atggcttgga taccgtcctc gtctgtcacg tcacagacat atccatgggc ttcgatgcca
    21781 acttcacgat aagcttcaat ccctttattg accagatctt gattgatatc gttaaagaca
    21841 atagtagcgc cggcttcagc gtaggatttg gcaatagcaa atccaattcc ataagaagcg
    21901 ccggtgatga gagcaatttt tccttttaaa gaaaactgct caattgagaa tgatgtattt
    21961 gtcatttgat acttcctttc aaatttgtta agacttctct ataaccaaat tatattctag
    22021 taaaccggtt ttgtcaataa ataaatattt ttatttataa aaaggtttgt tttcctaaaa
    22081 aaacggaaga ttttgaaatt tctgcctatt aaaatggtta aaaagataat gaaagcgcta
    22141 atattttttt aaaaaaacta ttgacaagat aaaaataaag gattacaatg gggaatataa
    22201 agtaaaccgg taaacctaaa caagaaagga gaaaagatga aaattgtact tgtagggcat
    22261 ggacattttg ctacagggat ttatagttct ttacaattga ttgcaggcaa tcaagaaaat
    22321 gtggaggcga ttgactttgt ggaaggaatg tcagcagatg aactcaagca aaaaatctta
    22381 cttgcaattt caaatgaaga agaagtttta atcctaagtg atctcttggg aggatcgcca
    22441 ttcaaggttt cttctaccat aatgggagaa aatccagcca aaacaatgaa tgttctctcg
    22501 ggtttgaact tagccatgtt aatggaagca gtctttgcta gaatggctca tagctttgat
    22561 gaggttgtta ataaatcagt agtggcggcc cagggcggag tcgtaaatgg taaagaattg
    22621 ttttcaacgg atgcagagga agaggaagaa gatttcgaat cgggtattta aagggtaaaa
    22681 gaatgataaa aaaggttacg attgaagaaa taaaatcgcc tgagcgcttc ttagaagtac
    22741 cacttctgac gaaagaagaa gtcggccagg caatcgataa ggttattcgg cagttagaac
    22801 tcaaccttga ctatttcaag gaagatttcc cgacgccagc tacctttgat aatgtctatc
    22861 caatcatgga taacacggaa tggaccaatg gtttctggac aggagaactg tggttggctt
    22921 atgaatacag tcaacaggat gcatttaaaa acatcgctca taaaaatgtt ctttctttcc
    22981 tggatcgtgt caataagaga gtagaattgg ctcaccatga tctcggcttc ttgtacacac
    23041 cgtcttgtat ggctgaatat aagataaatg gagatggaga ggctagagaa gcagccttga
    23101 aagctgcaga taagttgatt gaacgctatc aagaaaaagg tggttttatt caagcttggg
    23161 gagacttagg caagaaagag cattaccgtt tgattatcga ctgcttgctc aatatccaac
    23221 tcttattctt tgcttatcaa gaaacaggcg atcaaaaata ctacgatatt gcagaaagcc
    23281 atttctatgc ttcagctaat aatgtaatcc gtgatgacgc ttcgtccttc cacaccttct
    23341 attttgatcc tgagacaggt caacccttta aaggtgtaac gagacaaggg tatagtgatg
    23401 attcatgctg ggcacgtggt cagtcatggg gagtttatgg tattcctttg acttatcgtc
    23461 acttaaaaga cgagtcctgc tttgacttgt ttaagggtgt gaccaattat ttcttgaatc
    23521 gtctgccaaa agatcatgtg tcctattggg atttgatttt taatgatggt agtgatcaat
    23581 cacgagattc ttcagcaaca gctatcgccg tctgtgggat tcatgaaatg ctaaaacatc
    23641 tcccagaggt ggatgctgac aaagatattt ataaacatgc tatgcatgcc atgcttcgtt
    23701 ccttgatcga acattatgca aatgatcaat ttacccctgg tgggaccagt ctcctccacg
    23761 gtgtgtactc atggcattca ggtaaaggag tggatgaagg caatatctgg ggtgactact
    23821 attacctaga agcccttatc cgtttctaca aagactggaa cctatattgg taggaggaga
    23881 aatatgacaa tgccaaatat tattatgacc cgtatcgatg aacggttgat tcatggacaa
    23941 ggacaacttt gggtaaaata cctaggttgt aatacggtca ttgttgccaa tgatgaagta
    24001 agcacggaca agatgcaaca aactctgatg aaaacagttg tgccagactc agttgccatg
    24061 cgtttcttcc ctttgcaaaa ggtgattgat atcattcaca aggctaatcc tgctcaaacg
    24121 atctttatcg ttgtaaagga tgtgaaggac gctttaacct tggtggaagg tggtgtccct
    24181 atcaaagaaa tcaatattgg gaacattcac aatgctactg gtaaagagca agtgacacgc
    24241 tccatctttc tgggtgaaga ggacaaggcg gccctcaagg aattgagcca aactcatcaa
    24301 gtaacattta atacgaaaac aactccaaca ggaaatgatg gagctgttca agtcaacatt
    24361 atggactata tttaaaagag gagatcgtta tgtcgattaa tgtatttcaa gcgattttaa
    24421 ttggattatg gacagctttc tgttttagtg gaatgctgtt aggaatttac accaatagat
    24481 gcattgttct gtcatttggt gtcggaatta ttctaggtga tctgcctact gctcttgcaa
    24541 tgggagctat tggtgaattg gcttatatgg gattcggtgt tggtgctgga ggtactgttc
    24601 caccaaaccc aatcggacct ggtatctttg gtaccttgat ggctatcact agtgctggta
    24661 aagtcagtcc agaagcggct cttgccctct ctactccgat tgctgtggcg attcaattct
    24721 tacaaacttt cgcctacact gtacgtgctg gtgcgcctga aacagcgatg aagcacttga
    24781 aaaaccataa tttgaagaaa ttcaagttca ctctaaatgc aacaatttgg ttgtttgcct
    24841 ttattggatt taccttgggt tgcttgggtg ccctttcaat ggataccttg ttgaaactcg
    24901 tagactacat tccaccggta ttgcttacag gtttgacagt tgctggtaaa atgctcccag
    24961 ctatcggttt tgcgatgatc ttgtcagtga tggctaagaa agagttgatt ccctttgtct
    25021 tgttgggata tgtttgtgca gcttatctaa acatcccaac aattggtatt gcaattgtag
    25081 gtactatctt tgctttgatt gaattttata acaagccaaa aacagcggat catgtggtag
    25141 aggaggaagc acacgatgac tggatctaac aaattaacaa aacgtgatta tcttaaaacg
    25201 tctttgcggg cattcttttg tcaaaatgga tttaactata gtaactatca agggttggga
    25261 tatgccaatg tgatgtatcc tgctttgaaa aaacactatg gagaggatca ggaaggtttc
    25321 taccaagcct tggaagaaaa ctgtgaattc tataatacca acccacactt cctgcctttt
    25381 attaccagct tgcatcttgt aatgttggaa aatggccgtc cggcaaaaga aacacgtagc
    25441 atcaagatgg ccttgatggg accattggca ggtattgggg attctctttc tcaattctgt
    25501 ttagctccat tgttctcaac catcgcagct tcgtttgctc aagaaggctt ggttgtcggt
    25561 ccaatcttgt tcttccttgc gatgaatacg attttaacag cgattaaatt gtcaactggt
    25621 ctgtatggat acaaactagg aacaactgtg attgataaac taagcgaaca gatggcaacg
    25681 atttctcgta ttgccaatat tatcggtgta accgtaattg ctggtttggc agcgacatct
    25741 gttaagatta tggtgccgat tacctttgct gcaggggaag ttaaagcaga cgctaaacaa
    25801 agtatcgtaa gtattcaggg aatgcttgat aaggttgctc cagctcttct accagcccta
    25861 tttacacttt tagtttacta cttgatcaaa gaaaagaaat ggacaacata taaactcgtt
    25921 attttaacag ttatcatcgg aattatcgga agctggctta agattatagc ttaatggata
    25981 caacattgtt ttatggaata gtgattgtct tggcagtgag tcccctttta ctgtcaagct
    26041 ttcattctat tcgtcagcaa aagttgcttc gcaaacagat ggagcaacga caagagtatt
    26101 tagcttcttt aacatctggt gatgaagtgt tgttattgtc tggaattcat ggaaaaatca
    26161 tttctatcaa agatgacttg gtctccttgc agattgcaaa aggtgtggtc atctatgtag
    26221 aaaaggaaag tgtaatggga aagacaaaag aactgctttt taagtagttc tttttctggt
    26281 aatagataat gaaagaagaa agaagacaat tttttgaaag agtcgatgga aaccaatgtc
    26341 gtgattatat cttgtcgcac tgttcaaaag actatgagaa agtcaagagt tcccttgaac
    26401 gcttgatgga caatcgtttt atgtttgata gtccttggga tatggagcct tgttcaaaaa
    26461 tccatcaaat ccagccgatg gtatgggatc aagtatttga agatgatcca gaatgggctt
    26521 atatgctcaa tcgacaagaa tatctcttgc agtttatgat agggtatgtg gtagaaggag
    26581 ataaggacta tattcaaaag tgcaagttct ttctatttga ttggattgag caggtgagag
    26641 aattttctcc tcaatccttg atgactagaa ccttggatac gggtattcgt tcctttactt
    26701 ggttgaaact actcttgctc ctcttgaaat ttgacttgct agaggagaaa gaactagaga
    26761 aaattttggt cagtctagaa aagcagattg actttatgaa aagccactat cgcgccaagt
    26821 acacccttag taactggggg attttacaaa caattccgat gcttgctatc tatcatttct
    26881 tttcagataa gatggaccta gaagaagctt accatgttgc ttcagaggag ttgaaacagc
    26941 aaattgagac acagatttta ggagatggaa gccagtttga acagtcgatt ctctatcatg
    27001 tagaggttta taaagccttg ctggatttgt gtctcttgct tccagacttg caagatagtt
    27061 accaagagtt gctggaaaag atggcgacct atattcaaat gatgacaggc ttagatggac
    27121 ggactttggc ttttggtgat agcgattcta cagaaacgac agaaatgttg agcctgtctg
    27181 ctgtggtttt gaacaaggaa gaccttctta acggtctgga tgttaaagtc gatttgctta
    27241 gcctcttgtt cctggggcga gaaaaggtca agcgactgca ggaatttgaa aagagagctt
    27301 ggcagcctag gtccatgatc tttgaagact ctggacatgt ctgcattaag gatgaacatc
    27361 gttatctatt tttcaaaaat ggtccgctag gaagtgccca tagccatagt gacgagaata
    27421 gtttttgctt acagtatcaa ggccaaccta ttttcataga tgctgggcgt tattcttatc
    27481 gggagatata tgaacgttat ctcttaaaga gtgcttggag tcattcgacc tgcattgtag
    27541 atgggaaagc tccggaaaga atcacgggat cctgggaata tgaatactat cctcactccc
    27601 tgttttgtca ccataaagaa agggagggag tgcattatat tgaaggggct tattggtcag
    27661 cagaacctga tttgccttat cttcacaaga gaaaaatcct catgttggta gaggatgtct
    27721 ggctcttggt agatgacatc aggtgtcaag gtcagcatga ggcgttgact cagtttatcc
    27781 ttgacaagga tgtgacctat caagatggga aaatcaatca gttgagacta tggagtgaag
    27841 ttgattttga tttggaagat accatcattt ctcctaaata caatgagctt gaaagaagta
    27901 gcaaactcac caagcgccaa ttctttgaga atcagatgct ggattatacc atcattgcgc
    27961 atgagagttt tgaaatcatc cgtcattctg tctaccagac agatgatcgt gaagtggaaa
    28021 atgctctggc ttttgaagta aaaaatgacg aaacagacaa gctgattctg ttattaagcg
    28081 aggatatttg tgtaggtgaa aaattgtgcc tcgttgacgg aacaaaaatg cgtggaaaat
    28141 gtctagtata tgataaaata aatgagagaa tgattcgctt gcagtgctag aaataggcat
    28201 tttgaatagt gaatatgtta taataagtat tagtaggagg tgttttagat tggagaagaa
    28261 actgaccata aaagacattg cggaaatggc tcagacctcg aaaacaaccg tgtcatttta
    28321 cctaaatggg aaatatgaaa aaatgtccca agagacacgt gaaaagattg aaaaagttat
    28381 tcatgaaaca aattacaaac cgagcattgt tgcgcgtagc ttaaactcca aacgaacaaa
    28441 attaatcggt gttttgatcg gtgatattac caacagtttc tcaaaccaaa ttgttaaggg
    28501 aattgaggat atcgccagcc agaatggcta ccaggtaatg ataggaaata gtaattacag
    28561 ccaagagagt gaggaccggt atattgaaag catgcttctc ttgggagtag acggctttat
    28621 tattcagccg acctctaatt tccgaaaata ttctcgtatc atcgatgaga aaaagaagaa
    28681 aatggtcttt tttgatagtc agctctatga acaccggact agctgggtta aaaccaataa
    28741 ctatgatgcc gtttatgaca tgacccagtc ctgtatcgaa aaaggttatg agcattttct
    28801 cttgattaca gcggatacga gtcgtttgag tactcggatt gagcgggcaa gtggttttgt
    28861 ggatgcttta acagatgcta atatgcgtca cgccagtcta accattgaag ataagcatac
    28921 gaatttggaa caaattaaag aatttttaca aaaagaaatc gatcccgatg aaaaaactct
    28981 ggtatttatc cctaactgtt gggccctacc tctagtcttt accgttatca aagagttgaa
    29041 ttataacttg ccgcaagttg ggttgattgg ttttgacaat acggagtgga cttgcttttc
    29101 ttctccaagt gtttcgacgc tggttcagcc ctcctttgag gaaggacaac aggctacaaa
    29161 gattttgatt gaccagattg aaggccgcaa tcaagaagaa aggcaacaag tcttggattg
    29221 tagtgtgaat tggaaagagt cgactttcta aaatgaagga aaatgacttg caatctctgt
    29281 taagaaataa aataatccca cctagaacaa gctaggtggg attatttgcc tatgaaatga
    29341 gaaattatgg gagcaagctc ctaaatcaac tgtttttgat ctacttcttt aactacttga
    29401 taaaagttat agaagtaggc caaacttgaa atgatggtta cgactaggaa tattgaaaat
    29461 ttccattgga cagggttggt taaaagttgt ggaaaggata tgaggagaaa gaagagggct
    29521 gcgttgagga caggtatccg ttttgattgt attttctcaa gtcctttatt gagcgcagga
    29581 agaaagagga gtaggagtag taaaactgta tgagaaacag ctcctgaaat aagggcaaag
    29641 aaaaggaaaa tactgataaa aacatgaatg atcagtagtc tagctagtga tttcataagg
    29701 tacctcctaa tcctggtctt ttttagctct tgcaatacga agtgagtcga caatatgtat
    29761 catcactccg aaaaagaaag ctcccagtat agttttaaaa atatgttttg tatttagaag
    29821 agaactgata aaatttggat tttcacttgt tagggtatca atgagtggaa ttataaaaaa
    29881 tatcactgtt ccataaatcg aacctgcttt cagaccaggg taatgtagtt gcttactttc
    29941 tttctcgctc agcatgtctg gatcaatagc tgtaatccct gtttttttca tttggtaggt
    30001 gacatagcca gcagtgatga gggcaatcac taaaatcaga ggaggataga ttagagccac
    30061 ttcttgaggg tatttatagg ccagaaggag tggaataaga tttccaaaaa tcatcagata
    30121 aaagaggatg ataaagactt ggttcccaat acgatcggcc tcacgccgtt tgtattcgtc
    30181 aaggggacca gaaataccgt atgtgcgttt gatcagtttt tcagtgaagg tttctttttt
    30241 catgagtttg ctcctttttt aaaaatcttc ctcccaaaag agactgttga ggtcagtttg
    30301 gaggctgcgg gcgagattga gacagagttc caaggttgga ttgtacttgt cgttttcaat
    30361 catattgata gtctgtctcg agacaccgat atccttggcg agttcgagct gggaaatacc
    30421 caattccttg cgaaattctt tcacacgatt catctgttct cctttctgat ttgtgtcgta
    30481 tatatttgac tttattatag tcttttaaac ataaagtgtc aagtattttt gacatatttt
    30541 ttgaagaaat agtagtctcc ttgtcctatt tgtctgacaa gtgcaagctg gtcggatttg
    30601 tggtaaaata gataaaatat gacaaaagaa tttcatcatg taacggtctt actccacgaa
    30661 acgattgata tgcttgacgt aaagcctgac ggtatctacg ttgatgcgac tttgggcgga
    30721 gcaggacata gcgagtattt attaagtaaa ttaagtgaaa aaggccatct ctatgccttt
    30781 gaccaggatc agaatgccat tgacaatgcg caaaaacgct tggcacctta cattgagaag
    30841 ggaatggtga cctttatcaa ggataacttc cgtcatttac aggcacgttt gcgcgaagct
    30901 ggtgttcagg aaattgatgg aatttgttat gacttgggag tgtctagtcc tcaattggac
    30961 cagcgtgagc gtggtttttc ttataaaaag gatgcgccac tggacatgcg gatgaatcag
    31021 gatgctagtc tgacagccta tgaagtggtt aatcattatg actatcatga tttggttcgt
    31081 attttcttca aatacggtga ggataaattc tctaaacaga ttgcgcgtaa gattgagcaa
    31141 gcgcgtgaag tgaagccgat tgagacaacg actgagttag cagagattat caagttggtc
    31201 aaacctgcca aggaactcaa gaagaagggt catcctgcta agcagatttt ccaggctatt
    31261 cgaattgaag tcaatgatga actgggagcg gcagatgagt ccatccagca ggctatggat
    31321 atgttggctc tggatggtag aatttcagtg attacctttc attccttaga agaccgcttg
    31381 accaagcaat tgttcaagga agcttcaaca gttgaagttc caaaaggctt gcctttcatc
    31441 ccagatgatc tcaagcccaa gatggaattg gtgtcccgta agccaatctt gccaagtgcg
    31501 gaagagttag aagccaataa ccgctcgcac tcagccaagt tgcgcgtggt cagaaaaatt
    31561 cacaagtaag agggaaaaag atggcagaaa aaatggaaaa aacaggtcaa atactacaga
    31621 tgcaacttaa acggttttcg cgtgtggaaa aagcttttta cttttccatt gctgtaacca
    31681 ctcttattgt agccattagt attattttta tgcagaccaa gctcttgcaa gtgcagaatg
    31741 atttgacaaa aatcaatgcg cagatagagg aaaagaaaac cgaattggac gatgccaagc
    31801 aagaggtcaa tgaactatta cgtgcagaac gtttgaaaga aattgccaat tcacacgatt
    31861 tgcaattaaa caatgaaaat attagaatag cggagtaaga tatgaagtgg acaaaaagag
    31921 taatccgtta tgcgaccaaa aatcggaaat cgccggctga aaacagacgc agagttggaa
    31981 aaagtctgag tttattatct gtctttgttt ttgccatttt tttagtcaat tttgcggtca
    32041 ttattgggac aggcactcgc tttggaacag atttagcgaa ggaagctaag aaggttcatc
    32101 aaaccacccg tacagttcct gccaaacgtg ggactattta tgaccgaaat ggagtcccga
    32161 ttgctgagga tgcaacctcc tataatgtct atgcggtcat tgatgagaac tataagtcag
    32221 caacgggtaa gattctttac gtagaaaaaa cacaatttaa caaggttgca gaggtctttc
    32281 ataagtatct ggacatggaa gaatcctatg taagagagca actctcgcaa cctaatctca
    32341 agcaagtttc ctttggagca aagggaaatg ggattaccta tgccaatatg atgtctatca
    32401 aaaaagaatt ggaagctgca gaggtcaagg ggattgattt tacaaccagt cccaatcgta
    32461 gttacccaaa cggacaattt gcttctagtt ttatcggcct agctcagctc catgaaaatg
    32521 aagatggaag caagagcttg ctgggaacct ctggaatgga gagttccttg aacagtattc
    32581 ttgcagggac agacggcatt attacctatg aaaaggatcg tctgggtaat attgtacccg
    32641 gaacagaaca agtttcccaa cgaacgatgg acggtaagga tgtttataca accatttcca
    32701 gccctctcca gtcctttatg gaaacccaga tggatgcttt tcaagagaag gtaaaaggaa
    32761 agtacatgac agcgactttg gtcagtgcta aaacagggga aattctggca acaacgcaac
    32821 gaccgacctt tgatgcagat acaaaagaag gcattacaga ggactttgtt tggcgtgata
    32881 tcctttacca aagtaactat gagccaggtt ccactatgaa agtgatgatg ttggctgctg
    32941 ctattgataa taataccttt ccaggaggag aagtctttaa tagtagtgag ttaaaaattg
    33001 cagatgccac gattcgagat tgggacgtta atgaaggatt gactggtggc agaatgatga
    33061 ctttttctca aggttttgca cactcaagta acgttgggat gaccctcctt gagcaaaaga
    33121 tgggagatgc tacctggctt gattatctta atcgttttaa atttggtgtt ccgacccgtt
    33181 tcggtttgac ggatgagtat gctggtcagc ttcctgcgga taatattgtc aacattgcgc
    33241 aaagctcatt tggacaaggg atttcagtga cccagacgca aatgattcgt gcctttacag
    33301 ctattgctaa tgacggtgtc atgctggagc ctaaatttat tagtgccatt tatgatccaa
    33361 atgatcaaac tgctcggaaa tctcaaaaag aaattgtggg aaatcctgtt tctaaagatg
    33421 cagctagtct aactcggact aacatggttt tggtagggac ggatccggtt tatggaacca
    33481 tgtataacca cagcacaggc aagccaactg taactgttcc tgggcaaaat gtagccctca
    33541 agtctggtac ggctcagatt gctgacgaga aaaatggtgg ttatctagtc ggggtaaccg
    33601 actatatttt ctcggctgta tcgatgagtc cggctgaaaa tcctgatttt atcttgtatg
    33661 tgacggtcca acaacctgaa cattattcag gtattcagtt gggagaattt gccaatccta
    33721 tcttggagcg ggcttcagct atgaaagact ctctcaatct tcaaacaaca gctaaagctt
    33781 tggagcaagt aagtcaacaa agtccttatc ctatgcctag tgtcaaggat atttcacctg
    33841 gtgatttagc agaagaattg cgtcgcaatc ttgtacaacc catcgttgtg ggaacaggaa
    33901 cgaagattaa aaacagttct gctgaagaag ggaagaatct tgccccgaac cagcaagtcc
    33961 ttatcttatc tgataaagta gaggaagttc cagatatgta tggttggaca aaggagactg
    34021 ctgagaccct tgctaagtgg ctcaatatag aacttgaatt tcaaggttcg ggctctactg
    34081 tgcagaagca agatgttcgt gctaacacag ctatcaagga cattaaaaaa attacattaa
    34141 ctttaggaga ctaatatgtt tatttccctc agtgctggaa ttgtgacatt tttactaact
    34201 ttagtaggaa ttccggcctt tatccaattt tatagaaagg cgcaaattac aggacagcag
    34261 atgcatgaag atgtcaaaca gcatcaggca aaagctggga ctcctacaat gggaggtttg
    34321 gttttcttga ttacttctgt tttggttgct ttctttttcg ccctatttag tagccaattc
    34381 agcaataatg tgggaatgat tttgttcatc ttggtcttgt atggcttggt cggattttta
    34441 gatgactttc tcaaggtctt tcgtaaaatc aatgaggggc ttaatcctaa gcaaaaatta
    34501 gctcttcagc ttctaggtgg agttatcttc tatcttttct atgagcgcgg tggcgatatg
    34561 ctttctgtct ttggttatca agtgcatcta gggattttct atattgtctt cgctcttttc
    34621 tggctagtcg gtttttcaaa cgcagtaaac ttgacagacg gtgttgacgg tttagctagt
    34681 atttccgttg tgattagttt gtctgcctat ggagttattg cctatgtgca aggtcagatg
    34741 gatattcttc tagtgattct tgccatgatt ggtggtttgc tcggtttctt catctttaac
    34801 cataagcctg ccaaggtctt tatgggtgat gtgggaagtt tggccctagg tgggatgctg
    34861 gcagctatct ctatggctct ccaccaagaa tggactctct tgattatcgg aattgtatat
    34921 gtttttgaaa caacttctgt tatgatgcaa gtcagttatt tcaaactgac aggtggtaaa
    34981 cgtattttcc gtatgacgcc tgtacatcac cattttgagc ttgggggatt gtctggtaaa
    35041 ggaaatcctt ggagcgagtg gaaggttgac ttcttctttt ggggagtggg acttctagca
    35101 agtctcctga ccctagcaat tttgtattta atgtaagaat ggcaccctga tgtttcaggg
    35161 tgtttttgta tttaaataca caatgaaaat caaagaacaa actagaaagc taactttagg
    35221 ctgctcaaaa tatagtagat tgaaactaga atagtacaca tctgcttcta aaatattgtt
    35281 agaaatcgat ttgactgtcc tgatcgattt gtcctgttct tatttcattt taatatacag
    35341 ttttgagatg gttgatagaa ctgacgaagt cagctcaaaa cacggttttg aggttgtaga
    35401 taaggagaag ctgatgtggt ttgaagaggt tttcgaagag tataaaaaaa tattggtcag
    35461 ttaaagtcaa aactcttgac cttttctgac caatgtagta tattatgaac gtaaggtaaa
    35521 tgaaagcctt actagaacac ttatttaaag taaaatagaa agaggtgggt ctatgtttca
    35581 tctagaaatt ttcagaagta aagatggtct actcctgctt gaaaaagaaa aaccagaaat
    35641 agtacgtagg gtagcggttt agctagtcta aattttttaa aacaaaggtc aaagatagtc
    35701 aatatcagta atcataacta agtaaacaaa aagaggtaaa gaatatgaat aacaacttta
    35761 ataattttaa taacatggat gatttattta atcaattgat gggtggtatg cgaggataca
    35821 gttctgaaaa tcgccgttac ttaattaatg gacgcgaagt cacacctgag gaatttgctc
    35881 actatcgtgc gactggtcaa ttaccaggaa atgcagaaac tgatgtgcaa atgccacaac
    35941 aggcatcagg tatgaaacaa gacggtgtcc ttgcaaaact aggtcgaaac ttaacagcag
    36001 aagcgcgtga gggcaagttg gatcctgtta tcggacgaaa caaggaaatt caagaaacat
    36061 ctgaaatcct ctcacgccgc accaagaaca atcctgtttt ggtcggagat gcaggtgttg
    36121 gtaagacagc agttgtcgaa ggtctagcgc aagccattgt gaacggagat gttcctgctg
    36181 ctatcaagaa caaggaaatt atttctattg atatctcagg tcttgaggct ggtactcaat
    36241 accgtggtag ctttgaagaa aatgtacaaa acttagtcaa tgaagtgaaa gaagcaggga
    36301 atattatcct cttctttgat gaaattcacc aaattcttgg tgctggtagc actggtggag
    36361 acagtggttc taaagggctt gcggatattc tcaagccagc tctctctcgt ggagaattga
    36421 cagtgattgg ggcaacaact caagacgaat accgtaacac catcttgaag aatgctgctc
    36481 ttgctcgtcg tttcaacgaa gtgaaggtca atgctccttc agcagagaat acttttaaaa
    36541 ttcttcaagg cattcgtgat ctctatcaac aacaccacaa tgtcatcttg ccagacgaag
    36601 tcttgaaagc agcggtggat tattctgttc aatacattcc tcaacgtagc ttgccagata
    36661 aggctattga ccttgtcgat gtaacggctg ctcacttggc ggctcaacat ccagtaacag
    36721 atgtgcatgc tgttgaacga gaaatcgaaa cggaaaaaga caagcaagaa aaagcagttg
    36781 aagtagaaga ttttgaagca gctctaaact ataaaacacg cattgcagaa ttggaaaaga
    36841 aaatcgaaaa ccacacagaa gatatgaaag tgactgcaag tgtcaacgat gtggctgaat
    36901 ctgtggaacg aatgacaggt atcccagtat cgcaaatggg agcttcagat atcgaacgtt
    36961 tgaaagatat ggctcatcgc ttgcaagaca aggtgattgg tcaagataag gccgtagaag
    37021 ctgtagctcg tgctatccgt cgtaaccgtg ctggttttga tgaaggaaat cgcccaatcg
    37081 gcagcttcct ctttgtaggg tctactgggg ttggtaagac ggagcttgct aagcaattgg
    37141 cactcgatat gtttggaacc caggatgcga ttatccgttt ggatatgtct gaatacagtg
    37201 accgcacagc tgtttctaag ctaattggta caacagcagg ctatgtgggt tatgatgaca
    37261 atagcaatac cttaacagaa cgtgttcgtc gcaatccata ctctatcatt ctcttggatg
    37321 aaattgaaaa ggctgaccct caagttatta cccttctcct ccaagttcta gatgatggtc
    37381 gtttgacaga tggtcaagga aatacagtaa acttcaagaa cactgtcatt attgcgacct
    37441 caaatgctgg atttggctat gaagccaact tgacagaaga tgcggataaa ccagaattga
    37501 tggaccgttt gaaacccttc ttccgtccag aattcctcaa ccgctttaat gcagtcatcg
    37561 aattctcaca cttgactaag gaagaccttt ctaagattgt agatttgatg ttggctgaag
    37621 ttaaccaaac cttggctaag aaagacattg acttggtagt cagtcaagcg gctaaagatt
    37681 atatcacaga agaaggttac gacgaagtca tgggagttcg tcctctccgt cgagtggttg
    37741 aacaagaaat tcgtgataag gtgacagact tccacttgga tcatttagat gctaaacatc
    37801 tggaagcaga tatggaagat ggcgttttgg ttattcgtga gaaagcctaa gatagaattt
    37861 tgaggataaa aaagaaggag ccagctgaaa aaaaactggt tccttttgag gtacgacggg
    37921 catgtcgtat agtagaagtg tattattcta gtttcaattt actatagtag ctcagaagtc
    37981 ggtacttaaa cgtgctatat caaaaccagt ccttgaaaaa cgtggactgg tttcgtgttt
    38041 ggattattac cttgaacggc atgcgttaaa agttagttga accgccgtat gccgaacggc
    38101 acgtgcggtg gtgtgagagg ggctagagat tatcccctac tcgattttaa atcacatgac
    38161 gttcaaaggc atcatctgaa atcccttgtt ccaagatgag ttttgcccat tctttagcag
    38221 agaagaggct gtggtccttg tagtttccgc aagattcgat ggttgtccct gggacatctt
    38281 cccaagtagt agtttcagcg atttccttga gcgaatcctt gataacagct gcgattttag
    38341 cactggtgtg acgtccccac ataatcatgt ggaagcctgt gcggcaacca aatggcgaac
    38401 agtcaatcat gccgtcaatg cgggtacgga tgagtttggc taagaggtgc tcgatagtgt
    38461 gaaggccggc agtagggata gagtcttcgt ttggttgcac caagcgaata tcataattgg
    38521 agatgatgtc tccttttggt cctgtttctt ccccaatcaa gcgaacatag ggtgctttga
    38581 caatggtgtg gtcaagttca aaactttcga caataacttc ttttgacatg gtaaatcctt
    38641 tcagttttct tctctcatta tatcataaag gttgctcctg agacagagag aaaacctctc
    38701 cgaggctgga gaggttgaaa tctttactta cgatataagc ggtcgtattg gtagtatggg
    38761 tcaaaggtta cgttgatacc cagtttacga aggacattct tgtcttcatc agtcaagatg
    38821 atggttgagt gggcttcgct tcctttgagg ttgccgagtt cttccatagc gcgggcagca
    38881 tcaggatttt ctgtagctgt gatagcaagt gcaatcagaa tttcatttga atgaaggcgt
    38941 ggattgcggc taccgagatg atcgatttta agaccttgga ttggcttaac aacttcaggc
    39001 tcgattagtt ttacttcttt agcgatgtca gctgattttt tgatggcgtt gatcaaggca
    39061 gcggctgtag gcccaaagag ttctgagttc ttaccagtga cgatttcccc atttggcaat
    39121 tcaaaggcta gggctggtcc accagtttct tctgcttttt ggcgcgcaac gacagcaacc
    39181 ttacggtctg caggtgtgat accgaggtcg ttcatgagca acttaatttt cttgacggca
    39241 gcttcgccaa ctttttcagc tttgaagtca agaactgttt gatagtaacg gcggatgatt
    39301 tcttgtttag aagcttcgac agcagcctcg tcatctgtaa tagcgaaacc aaccatgttg
    39361 acacccatat ctgtcggtga agcgtatggt gattttccga gaatacgttc caacatgcgc
    39421 ttgagcactg ggaagatttc gatatcacgg ttgtagttga cagtggtttc tccataggtt
    39481 tgaagatgga aggggtcaat catgttgaca tcatcaaggt cagctgtggc agcttcataa
    39541 gccaagttaa ctggatgatg aaggggaaga ttccaaacag ggaaggtttc agatttagcg
    39601 tagccagact tgatgccatt gatttggtcg tggtacatat tggacataca cgttgccaat
    39661 tttccagaac cgggtccagg agcggttacg acaatcaagt tgcgactggt tttgatgtag
    39721 tcgtttttgc ccatgccttc tggggaaatg atgtgatcca tatccgtcgg atatcctttg
    39781 attggataat gaagataaga atcaattccg tttttctcaa gttgattgcg gaaggcatct
    39841 gcagcgggtt ggccagcgta ttgtgtaatg acaacggaac caacaaaaat ccctaattca
    39901 ttgaatttgt caatcaaacg aagaacttct tggtcataag aaatgcctaa gtcgccacgt
    39961 gctttggaat gttcaatgtt gctagcatta atggcaatca caacctcaac ctgctctttc
    40021 aattcttgca agagcttgat tttgttgtca ggttcataac caggaaggac acgagcagcg
    40081 tggaaatctt ctaacatttt accgccaaac tctaagtaga gcttgccgtc aaattggtta
    40141 atgcgctcta agatgtggtc gcgttgtaga tttaaatatt gttcagaact aaaagcttgt
    40201 tttttcattt ttttaccttt ggactctatt ataataaaaa attggaagtt aggaaactac
    40261 ggagctaaaa aagaaattaa aaagattaag caaacgcttg cacaaaattt taaaaagtgc
    40321 tatcatatac tatagattat taaaataatg aggtaaacag atgcaagaaa aatggtggca
    40381 taatgccgta gtctatcaag tctatccaaa gagttttatg gatagtaatg gagatggagt
    40441 tggtgatttg ccaggaatta ccagtaagtt ggactatcta gccaagctag gaatcacagc
    40501 aatttggctt tctcccgttt atgacagccc tatggatgat aatggctatg atattgctga
    40561 ttatcaagcg attgcggcta tttttggaac catggaggac atggatcagc tgattgcaga
    40621 agctaagaag cgtgatatcc gtatcatcat ggacttggtg gtcaatcata cctcggatga
    40681 gcatgcctgg tttgtagagg cctgtgaaaa tcctaatagc cctgagcgag actactatat
    40741 ctggcgggat gagcctaacg atttagattc tatctttagt gggtctgctt gggaatacga
    40801 tgaaaagtca ggtcaatact atctccactt tttcagcaag aaacagccgg atctcaactg
    40861 ggaaaatgaa aaacttcgcc agaaaattta tgagatgatg aacttctgga ttgataaagg
    40921 tattggtggt ttccgtatgg atgttattga catgattggc aaaattcctg acgagaaggt
    40981 agtcaataat ggtcctatgc tccatcccta tctcaaggaa atgaatcagg cgacctttgg
    41041 agataaggat ctcttgacag taggggagac ttggggagca acgccagaga ttgccaagct
    41101 ctactctgat ccaaaggggc aagaattgtc tatggtcttc cagtttgaac atatcggtct
    41161 tcagtatcag gaaggtcagc ctaaatggca ctatcaaaaa gagctgaata tcgctaagtt
    41221 aaaagaaatc ttcaacaaat ggcagacaga gttaggagtt gaggacggtt ggaattccct
    41281 cttctggaac aatcatgacc tccctcgtat cgtctcaatc tggggaaatg accaagaata
    41341 ccgcgaaaaa tctgccaaag cctttgcaat cttgcttcat cttatgagag gaactcctta
    41401 tatctaccaa ggtgaggaga ttgggatgac caactatccg tttgaaacac tggatcaagt
    41461 agaagatatt gaatctctca actatgcgcg tgaggctctt gaaaaaggtg ttccgattga
    41521 agaaatcatg gacagtatcc gtgttattgg acgtgacaat gcccgtaccc ctatgcaatg
    41581 ggacgagagc aaaaacgctg gtttctcaac aggtcaacct tggttggtgg ttaatccaaa
    41641 ttacgagatg atcaatgtcc aagaagcgct ggcaaatcca gattctattt tctataccta
    41701 tcagaaactg gtccaaattc gcaaggagaa tagctggcta attcgagctg actttgaatt
    41761 gcttgatacg gctgataagg tctttgctta tatacgtaag gatggcgacc gtcgcttcct
    41821 agttgtggct aacttgtcca atgaagagca agacttgaca gtagaaggaa aagtcaaatc
    41881 tgtcttgatt gaaaacaccc tagctcaaga agtctttgaa aaacaaatct tagttccatg
    41941 ggatgctttc tgtgtggaat tactataaat attttttgca gaaaaattta aaattgaaat
    42001 catataaaaa caaggaggac tgtataaaaa gcagaaatcc tttgtttttt ataaccaagg
    42061 tttataaact ttcattctcg aaattcaatt aactttacaa attcccacta ttaaggagaa
    42121 agaagatgaa cataaagaag cgtgtcctta gtgcaggcct gacttttgca tctgctttgc
    42181 ttttagctac ttgcggccaa tcaggttcag atacaaaaac ttactcatca acctttagtg
    42241 gaaatccaac tacatttaac tatctattag actattacgc tgataatata gtcaattgaa
    42301 acaagaacaa gacaaaagag cctcgtaaaa ggtattgcaa cttggtaata cctttttgag
    42361 gtgctttttg atatgagccc atgttttctc aataggattg tactcaggtg agtagggagg
    42421 aagaggtaaa agtttatgcc caaactcttc gcataaaagt tctagcttcc ccattctatg
    42481 gaatcttgta ttatccataa taataaccga tggtgtggtt aatgttggta agagaaactt
    42541 ctgaaaccaa gcttcaaaaa agtcgctcgt catcgtctct tcgtaagtca ttggagtgat
    42601 taactcccca tttgttagac ctgcaaccaa agaaatcctc tgatatcttc ttccagatac
    42661 tttgcctctt cttaactgac cttttaaaga gcgaccatat tctcgataaa aataaatatc
    42721 gaatcctgtt tcgtcaatct aaacaggtgc taggtgcttt aaactattaa aattcttaag
    42781 aaataaggct actttttctg ggtcttgttc ataattagat tgaaactaga gtagtacacc
    42841 tttgcttcta aaacattgtt agaaatcgat ttaactgtcc tgattgattt atcctattct
    42901 tgtttcaatc tactatattt ttgtttcgcg ggaagtctac ttaagatact taaagatgca
    42961 gatagtaaaa aagatgtaga cattaccgta aaaaagtgat ataatcgtat gatgttcaag
    43021 gtataggtgt taatcatgag tagacgtttt aaaaaatcac gttcacagaa agtgaagcga
    43081 agtgttaata tcgttttgct gactatttat ttattgttaa tttgtttttt attgttctta
    43141 atctttaagt acaatatcct tgcttttaga tatcttaatc tagtggtaac tgcgttagtc
    43201 ctactagttg ccttggtagg gctactcttg attatctata aaaaagctga aaagtttact
    43261 atttttctgt tgctgttctc tatccttgtc agctctgtgt cgctctttgc agtacagcag
    43321 tttgttggac tgaccaatcg tttaaatgcg acttctaatt actcagaata ttcaatcagt
    43381 gtcgctgttt tagcagatag tgagatcgaa aatgttacgc aattgacgag tgtgacagca
    43441 ccgactggga ctgataatga aaatattcaa aaactactag ctgatatcaa gtcaagtcag
    43501 aataccgatt tgacggtcaa ccagagttcg tcttacttgg cagcttacaa gagtttgatt
    43561 gcaggggaga ctaaggccat tgtcctaaat agtgtctttg aaaatatcat cgagtcagag
    43621 tatccagact acgaatcgaa gataaagaag atttatacca agggattcac taaaaaagta
    43681 gaagctccta agacgtctaa gaatcagtct ttcaatatct atgttagtgg aattgacacc
    43741 tatggtccta ttagttcggt gtcgcgatca gatgtcaata tcctgatgac tgtcaatcga
    43801 gataccaaga aaatcctctt gaccacaacg ccgcgtgatg cctatgtacc aatcgcagat
    43861 ggtggaaata atcaaaagga taaattgact catgctggta tatatggggt tgattcatcc
    43921 attcataccc tagagaacct ttatggtgta gatattaatt attatgttcg tttgaatttt
    43981 acctcatttt tgaaattgat tgacttattg ggaggggtag atgttcataa tgatcaagag
    44041 ttttcagctc tacatgggaa gttccatttc ccagtaggga atgtccatct agactctgag
    44101 caggctctag gttttgtacg tgaacgctac tcactagccg atggagaccg tgaccgtggt
    44161 cgcaaccaac aaaaggtgat tgtggctatc cttcaaaaat taacgtcaac cgaagcactg
    44221 aaaaattata gtacgatcat taatagcttg cacgattcca tccaaacaaa tgtgccactt
    44281 gagactatga taaatttggt caatgctcag ttagaaagtg gagggaatta taaagtaaat
    44341 tctcaagatt taaaaggtac aggtcggacg gatcttcctt cttatgcaat gccagacagt
    44401 aacctctatg tgatggaaat agatgatagt agtttagctg tagttaaagc agctatacag
    44461 gatgtgatgg agggtagatg agatgataga catccattcg catatcgttt ttgatgtaga
    44521 tgacggtccc aagtcaagag aggaaagcaa ggctcttttg gtagaagcct acagacaggg
    44581 ggtgcgaacc attgtctcta cctctcaccg tcgcaagggt atgtttgaaa ctccggaaga
    44641 gaagatagca gaaaactttc ttcaggttcg ggaaatagct aaggaagtgg cgagtgattt
    44701 agtcatcgct tacggggctg aaatttacta cacaccagat gttctggata agctagaaaa
    44761 aaagctgatt ccgaccctca atgatagtcg ttatgccttg atagagttta gtatgaacac
    44821 tccttatcgc gatattcata gcgccttgag caagatcttg atgttgggaa ttactccagt
    44881 cattgcccac attgagcgct atgatgctct tgaaaataat gaaaaacgcg ttcgagaatt
    44941 gatcgatatg ggctgttaca cgcaagtaaa tagttcacat gtcctcaaat ccaaactttt
    45001 tggcgaacgt tataaattca tgaaaaaaag agctcagtat tttttagagc aggatttggt
    45061 ccatgtcatt gcaagtgata tgcacaatct agacggcaga cctcctcata tggcagaagc
    45121 atatgacctt gttacccaaa aatacggaga agcgaaggct caggaacttt ttatagacaa
    45181 tcctcgaaaa attgtaatgg atcaactaat ttaggagaaa tgatgaaaga acaaaacacg
    45241 atagaaatcg atgtatttca attatttaaa accttgtgga aacgcaagct aatgatttta
    45301 ttagtggcac ttgtgacagg tgcgggggct tttgcatata gcgcttttat tgttaagcca
    45361 gaatatacga gtaccacgcg aatttacgta gtgaatcgcg atcaaggaga caagtcgggg
    45421 ctgacaaatc aggatttgca ggcaggatct tatctggtaa aagactaccg tgagattatc
    45481 ctttcgcaga atgtattgga aaaagtagcg acaaatttga agttggatat accagccaaa
    45541 acgttagcta gaaaagttca agtgactgta ccagttgaca ctcgtatcgt ctcaatctct
    45601 gtcaaggata aacagccaga ggaagccagt cgtatcgcta attctctacg agaagttgct
    45661 gcagaaaaga tcatcgctgt aacgcgagta tctgatgtga cgacacttga agaagcgcga
    45721 ccagctacga ctccctcttc tccaaatgtt ggacgcaatt ccttgtttgg tttttttgga
    45781 ggagcagtcg taacagtaat tgctgttctt ttgattgagt tgttcgacat ccgtgtgaaa
    45841 cgtcctgaag atgtcgaaga tgtactgcaa attccacttt taggggtcgt tccagatttg
    45901 gacaaaatga aataggagga agttatgcca acattagaaa tctcacaggc aaaattggat
    45961 tttgtaaaaa aggcagagga aaattataac gctttgtgca cgaacctaca gttaagtgga
    46021 gatgatttga aagtattttc tatcacttct gtgaaacaag gagaaggaaa atcaacgact
    46081 tccaccaata tcgcttgggc ttttgcgcgt gcaggttaca aaacgctgct gattgatgga
    46141 gatattcgca attctgttat gttaggtgtc tttaaagcaa gagataagat tacaggcctg
    46201 acagaatttt tatcaggaac tacagaccta tcacaagggc tttgtgatac caatatcgaa
    46261 aatctctttg taattcaggc tggctctgtg tcaccgaatc cgacagctct tcttcaaagt
    46321 aagaatttca gtacaatgct tgaaaccttg cgtaaatatt ttgactacat cattgtagat
    46381 actgctcctg tcggtgtcgt gattgatgcg gctattatta cgcaaaaatg cgatgcttct
    46441 attttagtga cgaaggcagg tgaaataaat cgacgggata ttcaaaaagc aaaagaacag
    46501 ttggaacaca cagggaagcc gtttttggga gttgtgttga ataaattcga tacttcagta
    46561 gacaaatacg gttcttatgg aaattacgga aaaaaataag tcgttaagta tttgtgtaag
    46621 gacattttta gaactacaat caaaatcttt ggaattgctg gcttcaaatc atattgattt
    46681 gaaatgaaac ttgcaaaaga gtttttaagg tgagtttctt aatttgtgac tgaaatcaat
    46741 cttctacttc cataccttgg aaaaacaggg taaacgagag atgttttttc cataataaag
    46801 ttttgatatg gctagtagag tagtaatata atgaattgta atcattagtg ttcataccaa
    46861 gaaatatcaa gtatctattt ttgaaaatca taaatatgag atagttttta aatctctaag
    46921 gagagctagt tttgagaggt aatttatgta tagagtaaca aaacgattag gggatatttt
    46981 actttcttta ataggaataa tagtactgtt tccagtgttt attgtaattg caattgcgat
    47041 aaacactgat tcggagggtc cgattatatt taaacaaaaa cgatttggga tatacaagga
    47101 acctttttat atcctgaaat ttaggtccat gaaaatagga gcaccgaaaa atgtagctcc
    47161 acggaacttg gataatccag aacagtggat taccaaagtg ggagctttct taagaaaaac
    47221 atctttggac gaactgccac aattatggaa tattcttatt ggtaatatga gcattgtagg
    47281 acctaggcca gcaggtataa atgagcttga tttgctcacc gaaagagaaa agtttggtgc
    47341 gaatgatgtc ttacctggat taactggatg ggcacaaatt aatgggcgag atactttgtc
    47401 aatagagatg aaggcagaag cagatggtta ttatgtgaaa catatgtcgt taagcatgga
    47461 tcttagatgt atcattagga caatccccta cgtattaaaa cgtaagggaa ttgtggaggg
    47521 cagtggtaag aaagagttgg attgatgcaa tgaaaattct atttgtttgt caacattata
    47581 agccagaacc ttttaggttg tcagatattt gtgaggatct agttcaaaga gggcatgagg
    47641 tggctgtttt gacagggatt cctaactatc ctgaggggga aatatatgct gattatcgta
    47701 agagaaaaaa aagaagagag actataaatg gtgttgctat ttttcgttca tacaccattg
    47761 ctaggaggca aaatacttta taccgtatat tgaattattt tagttttgct ctttcttcta
    47821 cgataggtgt tatatttggg cgttataaag cgaaagatgg ctcagatttt gattgtgtct
    47881 ttgtcaatca actatctcca gttatgatgg cttgggctgg tatagcttat aagaacaagt
    47941 acaataaacc tatgtttttg tattgcatgg atgtttggcc ggatagttta attgtaggtg
    48001 gggtgaagga aaatggattg atttacaaga tatttgaatt tgtttcaaaa aaagtatatc
    48061 aagccagcga ttatatattt gttactagtc tatcttttaa agattatttt gtaaaaaaat
    48121 ttaatattcc tctccacaaa attacttact taccacaata tgcagaggat ttatttgtac
    48181 caaatgaatt aaaaacgaac aaaaatacta ttgacttaac ttttgctgga aatattggaa
    48241 aagctcagaa tttagagact attttgaaag cagccagtgc aatagaacag attcccgatt
    48301 tagcaaagag agttcatttt cattttgttg gagatggtac ggaactatta aatatgcaaa
    48361 aattagcatg tgaactagaa ttagagaaca cttcatttta tggtagacgt ccattagaag
    48421 agatgccgga tttttataca aaatcagatg cgatgctagt ttctttaata ggcgactcta
    48481 taatttctcg caccttacca ggcaaggtac aatcttatat ggcggcagga aaaccaatta
    48541 taggagcgat ttcaggagat actcagagag ttgtaaaaga agcaaaatgt ggtttcataa
    48601 gtccggaagg gaatgttgat cagttagtga gaaatattcg taagttctgt ttgctttctg
    48661 tagaggaaag agagaaacta ggaaggcaag ctcgttgtta ttatgaagaa cagttttcga
    48721 aagagtggtt tatgacatac ttagaaaatc atttaaaaga ggggtttcta tcatgaaggt
    48781 tttaatgatt aattccgttt gtggtattag gagcactggg agaatttgtg cagatctcgc
    48841 aactgctttg acagaagaag gatatgaggt taaaatcgcc tatggtcgtg agtatgttcc
    48901 gaaacaattt caacagtatg cagtgaaaat tggtacaaag tctgatattc gttttcatgc
    48961 gattcgttct cgattatttg acgaatctgg ctttggaagt agacaggcaa ctgttaattt
    49021 tattaaatgg atgaaagagt ttgatcctga tattattcat ctccataacc ttcatgggta
    49081 ttatatcaat gttgaactgt tatttgatta tctccgaata tgcggtaaaa agattatatg
    49141 gactttgcat gattgttggg catttacagg acactcggct tattttgatt gtttggaatg
    49201 tgagtcaaag ggagtttgtg accatccatc tcagaaaaat gattatccca agtcgttcgt
    49261 gaatttttca aagcgaaatt atcaaagaaa gaaaaaatgt tttactcata ttcctaatct
    49321 cactattgtt actccgtctc aatggctagc taatttagta agacagtcat ttttaaaaga
    49381 atatccagtg aaagtaattc ataatggtat tgatactact gtctttcaac cgaggctaat
    49441 agccgctcaa aaattgagaa aaagacacca tcttgaagga aagcaggtac tgcttggtgt
    49501 ggctgctatc tgggataata gaaagggatt ttctgatatg ttagcgctcg cgaatgatct
    49561 aactgataat cagagaatag ttttgatagg gttaaaagat aggcaactca aagaactacc
    49621 caaagggatc attgggttaa cagaaactaa tagcccagaa gagttggtag catggtatac
    49681 actagcggat gtatttctaa atcctactta tcaagataat tacccgacaa ctaatttaga
    49741 agctatttct tgtgggacac cagttatcag ctatccgaca ggtggtagtg tagaaagtgc
    49801 taaactttat ggtctcgttt gtcaggatag aaacgtagct tctattttat cttcgttaga
    49861 aaaagtctct cagctatcta aaacagaaaa attggatttt tcaattgcaa gttttattaa
    49921 acaaatgaaa tatttatatt agaac
//
LOCUS       DAVKJD010000007        46734 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKJD010000007 DAVKJD010000000
VERSION     DAVKJD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46734
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.007"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..375
                     /locus_tag="VW157_000415"
     CDS             <1..375
                     /locus_tag="VW157_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9551771.1"
                     /translation="NGSMATGWLQYNGSWYYLNANGSMATGWLQNNGSWYYLNSNGAM
                     VTGWLQYNGSWYYLNANGSMATDWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGS
                     GALAVNTTVDSYRVNANGEWVN"
     gene            complement(605..1585)
                     /gene="dusB"
                     /locus_tag="VW157_000416"
     CDS             complement(605..1585)
                     /gene="dusB"
                     /locus_tag="VW157_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9551772.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1572..2444)
                     /gene="hslO"
                     /locus_tag="VW157_000417"
     CDS             complement(1572..2444)
                     /gene="hslO"
                     /locus_tag="VW157_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9551773.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2609..4036
                     /locus_tag="VW157_000418"
     CDS             2609..4036
                     /locus_tag="VW157_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9551774.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLTQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            4195..5703
                     /gene="glpK"
                     /locus_tag="VW157_000419"
     CDS             4195..5703
                     /gene="glpK"
                     /locus_tag="VW157_000419"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9551775.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGSAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5745..7571
                     /gene="glpO"
                     /locus_tag="VW157_000420"
     CDS             5745..7571
                     /gene="glpO"
                     /locus_tag="VW157_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9551776.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKIYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDNKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7641..8345
                     /locus_tag="VW157_000421"
     CDS             7641..8345
                     /locus_tag="VW157_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9551777.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAYLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVFVFSLF"
     gene            8642..8845
                     /locus_tag="VW157_000422"
     CDS             8642..8845
                     /locus_tag="VW157_000422"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551778.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8932..9540)
                     /locus_tag="VW157_000423"
     CDS             complement(8932..9540)
                     /locus_tag="VW157_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551779.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            10632..11909
                     /gene="ltrA"
                     /locus_tag="VW157_000424"
     CDS             10632..11909
                     /gene="ltrA"
                     /locus_tag="VW157_000424"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEU9551780.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAASKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKFKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSLGNMK
                     SIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGIPKWITDKVSGWGDHYQLVAQK
                     SVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            12364..13542
                     /locus_tag="VW157_000425"
     CDS             12364..13542
                     /locus_tag="VW157_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9551781.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            13856..13987
                     /locus_tag="VW157_000426"
     CDS             13856..13987
                     /locus_tag="VW157_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9551782.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            14008..15558
                     /gene="dltA"
                     /locus_tag="VW157_000427"
     CDS             14008..15558
                     /gene="dltA"
                     /locus_tag="VW157_000427"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9551783.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMKNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            15555..16799
                     /gene="dltB"
                     /locus_tag="VW157_000428"
     CDS             15555..16799
                     /gene="dltB"
                     /locus_tag="VW157_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9551784.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELIDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHVLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            16813..17052
                     /gene="dltC"
                     /locus_tag="VW157_000429"
     CDS             16813..17052
                     /gene="dltC"
                     /locus_tag="VW157_000429"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9551785.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            17045..18313
                     /gene="dltD"
                     /locus_tag="VW157_000430"
     CDS             17045..18313
                     /gene="dltD"
                     /locus_tag="VW157_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9551786.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFIPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            18468..18908
                     /gene="adcR"
                     /locus_tag="VW157_000431"
     CDS             18468..18908
                     /gene="adcR"
                     /locus_tag="VW157_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9551787.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            18908..19612
                     /locus_tag="VW157_000432"
     CDS             18908..19612
                     /locus_tag="VW157_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9551788.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            19605..20411
                     /locus_tag="VW157_000433"
     CDS             19605..20411
                     /locus_tag="VW157_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9551789.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            20421..21926
                     /gene="adcA"
                     /locus_tag="VW157_000434"
     CDS             20421..21926
                     /gene="adcA"
                     /locus_tag="VW157_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9551790.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGKEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVPNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(23086..23859)
                     /locus_tag="VW157_000435"
     CDS             complement(23086..23859)
                     /locus_tag="VW157_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9551791.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            24035..25438
                     /locus_tag="VW157_000436"
     CDS             24035..25438
                     /locus_tag="VW157_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9551792.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKEE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            25553..26191
                     /locus_tag="VW157_000437"
     CDS             25553..26191
                     /locus_tag="VW157_000437"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEU9551793.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            26203..26643
                     /locus_tag="VW157_000438"
     CDS             26203..26643
                     /locus_tag="VW157_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEU9551794.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSVSCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            26636..27067
                     /locus_tag="VW157_000439"
     CDS             26636..27067
                     /locus_tag="VW157_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9551795.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            27058..27528
                     /locus_tag="VW157_000440"
     CDS             27058..27528
                     /locus_tag="VW157_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9551796.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKIRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            27553..28326
                     /locus_tag="VW157_000441"
     CDS             27553..28326
                     /locus_tag="VW157_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9551797.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            28323..29123
                     /locus_tag="VW157_000442"
     CDS             28323..29123
                     /locus_tag="VW157_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9551798.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            29182..31476
                     /locus_tag="VW157_000443"
     CDS             29182..31476
                     /locus_tag="VW157_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9551799.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKGCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFINRVKELKKKLPRTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQDREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            31486..34602
                     /locus_tag="VW157_000444"
     CDS             31486..34602
                     /locus_tag="VW157_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9551800.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVINRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKLTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSDFDVILYDNNKNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYSHHSVTHTKEDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNDYEVFTQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            34685..36451
                     /locus_tag="VW157_000445"
     CDS             34685..36451
                     /locus_tag="VW157_000445"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9551801.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISLSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGYTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            36597..37748
                     /locus_tag="VW157_000446"
     CDS             36597..37748
                     /locus_tag="VW157_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9551802.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKFIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMAINGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAITEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            37892..38776
                     /locus_tag="VW157_000447"
     CDS             37892..38776
                     /locus_tag="VW157_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9551803.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(38894..38971)
                     /locus_tag="VW157_000448"
                     /pseudo
     CDS             complement(38894..38971)
                     /locus_tag="VW157_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            39186..39437
                     /locus_tag="VW157_000449"
     CDS             39186..39437
                     /locus_tag="VW157_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9551804.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFDGMGDQPVQDTE
                     TSSALILSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            39425..39715
                     /locus_tag="VW157_000450"
     CDS             39425..39715
                     /locus_tag="VW157_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551805.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(39880..40007)
                     /locus_tag="VW157_000451"
                     /pseudo
     CDS             complement(39880..40007)
                     /locus_tag="VW157_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(40102..41433)
                     /locus_tag="VW157_000452"
     CDS             complement(40102..41433)
                     /locus_tag="VW157_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9551806.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEENLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     LLYEQVCNGLKEAGYDYQTTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(41455..42966)
                     /locus_tag="VW157_000453"
     CDS             complement(41455..42966)
                     /locus_tag="VW157_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9551807.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            complement(43177..44124)
                     /gene="arcC"
                     /locus_tag="VW157_000454"
     CDS             complement(43177..44124)
                     /gene="arcC"
                     /locus_tag="VW157_000454"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9551808.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVEADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            complement(44289..45305)
                     /gene="argF"
                     /locus_tag="VW157_000455"
     CDS             complement(44289..45305)
                     /gene="argF"
                     /locus_tag="VW157_000455"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9551809.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDAHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            complement(45363..46592)
                     /gene="arcA"
                     /locus_tag="VW157_000456"
     CDS             complement(45363..46592)
                     /gene="arcA"
                     /locus_tag="VW157_000456"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9551810.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDESKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
BASE COUNT        14336 a         8341 c        10010 g        14047 t
ORIGIN      
        1 aatggttcaa tggcgacagg atggctccaa tacaatggtt catggtacta cctcaacgct
       61 aatggttcaa tggcgacagg atggctccaa aacaatggtt catggtacta cctaaacagc
      121 aatggtgcta tggtaacagg atggctccaa tacaatggtt catggtacta cctaaacgct
      181 aacggttcaa tggcaacaga ttgggtgaaa gatggagata cctggtacta tcttgaagca
      241 tcaggtgcta tgaaagcaag ccaatggttc aaagtatcag ataaatggta ctatgtcaat
      301 ggctcaggtg cccttgcagt caacacaact gtagatagct atagagtcaa tgccaatggt
      361 gaatgggtaa actaaaccta atataactag ttaatactga cttcctgtaa gaactcttta
      421 aagtattccc tacaaatacc atatcctttc agtagataat atacccttgt aggaagttta
      481 gattaaaaaa taactctgta atctctagcc ggatttatag cgctagagac tacggagttt
      541 ttttgatgag gaaagaatgg cggcattcaa gaggctcttt aagagagtta cgggttttaa
      601 actattaagc cttctccaat tgcaagaggg cttcaatctc tgccagggtg ctagcttgcg
      661 aaatggctcc acggagtttg gcagcgccag atgttccacg gaggtagtga ggagcgaggc
      721 cgcggaattc acgaactgcg acgttttctc ctttgaggtt aatcaatcgt ttcaagtgtt
      781 cgtaggcgat cttcatcttg tcttcaaagg tcaaatcagg taggatttct cctgtttcaa
      841 agtaatggtt gatttggttg aagaggtaag gatttcccat ggcagctcgg ccaatcatga
      901 ctgcgtcagc accaacttct tcgatgcgtt gcttggcttc ttggacagta cggatatcac
      961 cgttggcgat gaatggaatc ttggttagag cttgggcaac cttgtaaagg gtctcaaggt
     1021 ctgcgtggcc agtatacatt tgttcacggg tacggccatg catggcgagg gcagaaacac
     1081 ctgcagcctc agcagcgagg gcattttcta ctgccagaga tgggtccgcc cagccggtac
     1141 gcattttgac agtaagtggg atatcaagga cagactggac cttgttgatg atagagtaga
     1201 tcttgtcagg atccttgagc cacatagctc cagcttcgtt cttcacgatt ttgttgacag
     1261 ggcagcccat gttgatatcg acgatatcgg tcttggtgtt ttcttggatg aattctgctg
     1321 cgcgtgctag gctgtcttca tcgctaccaa aaagttggat agagacaggg ttttcgccct
     1381 catcgatatg aagcatgtgc agggtttttt cgttgttgta ttggattccc ttgtcagaga
     1441 ccatttccat tacaacgagt ccagctccga gctcttttgc gatggtacga aaggctgagt
     1501 tggtcacgcc agccataggc gctaaaacgg tacgattggg aatctcaaca ttgccaatca
     1561 taaaaggtgt attaagattt gtcacgaatg agttcctcca ggtccttttc atcaaagttg
     1621 taagtagttt ggcagaattg acaagtgatt tctaccccgt ggtcttcctc tttcatttcc
     1681 tgtaagtctg agcttggaag gctggcaaga gcgttcataa agcgttcatg gctacagtca
     1741 cattggaaac ggatttcttc ttcagaaaga cgcttgtagg cttcgtcccc atagatagcc
     1801 ttgaggaggg cttcgatatg gtcgtcgctt tcgagaagag tagagatagc tggcatttct
     1861 tggatgcgtt tttcaaagcg agcaatctct tctttcttgg ctcctggcaa gacttgaact
     1921 aggaaaccac ctgcaacctt gaccttgtct tcctcgtcca aaaggacatt gaggccgacc
     1981 gctgaaggcg tttgttggct ttcagtaagg taaaaggcaa ggtcttcacc gatttctcca
     2041 gagatgaggg gagttataga gttgtaagga tttccagtac cgtagtctgt gataacgagg
     2101 aattgaccat ttccaacaaa aggtccgact aggacttcac cagtcgcagt ctttttgatg
     2161 tcaacaccag gattttgaac atagcctttg acgttcccct tggtatcagc gacggtgata
     2221 atagcaccta gagagctaga gcccaacacc ttaactgtaa gtttggtatt tcctttttca
     2281 ttggctgcga gaatctggct ggcgataaga gttcgaccaa gcgctacagt tgagctagct
     2341 tgggtttgat gtttttcttg agcagtgcgg acggtttccg tgctatcaag gacaaaagca
     2401 cgaaaggctc cgctttctga tatagtttta ataattttat ccatagctac tattttagca
     2461 taaaaatgcc caaaggggga gccgtgtgtt tactgatttt caggataatg gaccaggaaa
     2521 tcagcatgaa aataaaaaga gaaacagatt attttagcat ttgtaagatt tatgctatgc
     2581 ttaaggtaga aaatgaaagg ggtaacaaat gtatttagga gatttgatgg agaaagccga
     2641 gtgtggtcaa ttttcaatcc tttcctttct attacaagag tctcagacga ccgtcaaggc
     2701 tgtaatggaa gaaacaggat tttcaaaagc aaccctaacc aaatatgtca ccctgctcaa
     2761 tgacaaggct ttggatagtg gcttagagct ggctattcac tcagaagatg aaaatctgcg
     2821 tctgtctatc ggtgcagcta ccaaggggag agatattcgg agcttgtttt tggagagtgc
     2881 tgttaaatac cagattttgg tttatcttct ctaccaccaa cagtttttag cccatcagct
     2941 gactcaagaa ttggtgatta gcgaggctac gcttggtcgt cacttggctg gtttaaatca
     3001 gattttgtca gaatttgatt tatccatcca aaatggacgt tggcgaggtc cagagcatca
     3061 gattcgctat ttctatttct gtcttttccg aaaggtctgg tcgagtcagg aatgggaagg
     3121 tcacatgcag aaaccagaga gaaaacagga gattgccaat ttagaggaaa tctgcggtgc
     3181 aagtttgtct gtggggcaga aattggactt ggttctctgg gctcacatca gtcaacaacg
     3241 tcttcgggtc aatgcttgtc agtttcaagt catagaagag aaaatgcgag ggtattttga
     3301 caatattttc taccttcgtt tgctgagaaa ggttccgtcc ttttttgctg ggcaacatat
     3361 tccactagga gttgaggatg gtgagatgat gatattcttc tcttttctcc tatctcatcg
     3421 cattcttcct cttcatacta tggagtatat tcttggtttt ggagggcagt tggcagattt
     3481 actgacgcaa ttgattcaag aaatgaagaa ggaggaacta ttgggggatt atacagagga
     3541 ccatgtcacc tatgaactca gtcagctttg tgctcaagtc tatctctata agggctatat
     3601 tttacaggat cgctacaagt accagttaga gaatcgtcat ccatatttac tgatggaaca
     3661 tgattttaaa gagacagcag aggagatttt tcatgctcta cctgcttttc aacaggggac
     3721 agatttagat aagaagattc tctgggaatg gctccagtta atcgaatata tggctgaaaa
     3781 cggtggccag catatgcgga ttggtctgga tttgacatct ggttttcttg tcttttcaag
     3841 gatggcagcc attttgaaac ggtatttgga atacaatcgt tttattacca ttgaagctta
     3901 tgaccctagt cggcattatg atttgctggt taccaataac ccgattcata agaaggaaca
     3961 gacaccagtc tattatttaa aaaatgactt ggatatggag gatttggcag ggattcgtca
     4021 gttattattc acttaaaagg cttggttaat ccaggtcttt tttgtgaaat tcacacaatc
     4081 tcctcacatt tttttaaaaa ttaaaaaaag ttgataaaca agaaagcgct ttattttgta
     4141 tactagtaag tgtaaagagg aaacacctca agatctttat caggaggaca gtatatgtca
     4201 caagaaaaat acatcatggc cattgaccag ggaactacaa gttctcgtgc catcattttc
     4261 aacaaaaaag gggaaaaggt tagctcgagt caaaaagagt ttacccagat tttccctcag
     4321 gcaggttggg ttgagcacaa tgccaatgaa atttggaact ctgttcagtc agttattgcg
     4381 ggtgctttca tcgaaagtgg tgtcaagcca aatcaaatcg aggcaatcgg gattaccaac
     4441 caacgtgaaa caacggttgt ctgggataag aaaacaggac ttcctatcta caatgctatc
     4501 gtttggcagt cacgccagac agcacctttg gctgagcaac taaaaagcca aggttatgtg
     4561 gaaaaattcc atgaaaagac tggtttgatt attgatgctt acttctctgc taccaaggtt
     4621 cgttggattt tggatcatgt agaaggcgct caagagcgag cagaaaaagg ggaattgctc
     4681 tttggtacta tcgatacttg gttggtttgg aaattgactg acggtgcggc tcacgtgact
     4741 gactactcaa atgcagctcg taccatgctt tataacatta aagaactcaa atgggatgat
     4801 gaaattttgg aaatccttaa cattccgaag gctatacttc cagaagttcg ttctaactcc
     4861 gaaatctacg gcaagacagc tccattccat ttctacggtg gagaggtgcc aatctcaggt
     4921 atggctgggg accaacaagc agccctcttt ggacagttgg cttttgagcc aggtatggtt
     4981 aagaatactt atggaacagg ctctttcatc atcatgaata ctggggaaga gatgcagttg
     5041 tctgaaaaca acctcttgac aaccattggt tacggaatca acggtaaggt ttattatgcc
     5101 ttggaaggtt ctatcttcat cgcaggaagt gctattcagt ggcttcgtga cggtcttcgc
     5161 atggttgaaa attcaccaga atctgaaaaa tacgctcgtg attctcacaa caacgatgaa
     5221 gtttatgtcg ttccagcctt tacaggtcta ggcgctccat actggaacca aaatgctcgt
     5281 ggttccgtct ttggtttgac tcgtggaaca agcaaagaag actttatcaa ggcgactttg
     5341 caatctattg cttatcaagt gcgtgacatc atcgacacca tgcaagtgga tactcagacc
     5401 gccattcaag tactgaaggt ggatggtagt gcagccatga acaacttcct catgcaattc
     5461 caggcggata ttttaggcat tgacattgca cgtgctaaaa acttggaaac aacagctcta
     5521 ggagcggcct tcctagcagg tttgtcagta gggtactgga aagacttgga cgagttgaaa
     5581 ctcttgaacg agacaggaga actctttgag ccatctatga atgaatctcg caaggaacaa
     5641 ctctacaagg gctggaagaa ggctgtgaaa gcaactcaag tctttgcgga agtagacgac
     5701 taatactggc agaataaagc gatttattta gaaagtgtgt aaatatggaa ttttcaaaga
     5761 aaacacgtga attgtcaatt aaaaaaatgc aggaacgcac tctggacctc ttgattatcg
     5821 gtggaggaat cacaggagct ggtgtagcct tgcaggcggc agctagcggt cttgagactg
     5881 gtttgattga aatgcaagac tttgcagaag gaacatctag tcgttcaaca aaattggttc
     5941 acggaggact tcgttacctc aaacaattcg acgtcgaagt ggtctcagat acggtttctg
     6001 aacgtgcagt ggttcaacaa atcgctccac acattccaaa accagaccca atgctcttac
     6061 cagtttacga tgaagatgga gcaaccttta gcctcttccg tcttaaagta gccatggact
     6121 tgtacgacct cttggcaggt gttagcaaca caccaactgc gaacaaggtt ttgagcaagg
     6181 atcaagtctt ggaacgccag ccaaacttga agaaggaagg cttggtagga ggtggagtgt
     6241 atcttgactt ccgtaacaac gatgcgcgtc tcgtgattga aaacatcaaa cgtgccaacc
     6301 aagacggtgc cctcattgcc aaccacgtga aggcagaagg cttcctcttt gacgaaagtg
     6361 gcaagattac aggtgttgta gctcgtgatc tcttgacaga ccaagtattt gaaatcaagg
     6421 cccgtctggt tattaataca acaggtcctt ggagtgataa agtacgtaat ttgtctaata
     6481 agggaacgca attctcacaa atgcgcccaa ctaagggagt tcacttggta gtggattcaa
     6541 gcaaaatcaa ggtttcacag ccagtttact ttgacacagg tttgggtgac ggtcgtatgg
     6601 tctttgttct cccacgtgaa aacaagattt actttggtac aactgataca gactacacag
     6661 gtgatttgga gcatccaaaa gtaactcaag aagatgtaga ttatctactt ggcattgtca
     6721 ataaccgctt tccagaatcc aacatcacca ttgatgatat cgaaagcagc tgggcaggtc
     6781 ttcgtccatt gattgcaggg aacagtgcct ctgactataa tggtggaaat aacggtacca
     6841 tcagtgatga aagctttgac aacttgattg cgactgttga atcttatctc tccaaagaaa
     6901 aaacacgtga agatgttgag tctgctgtca gcaagcttga aagtagcaca tctgagaaat
     6961 atttggatcc atctgcagtt tctcgtgggt ctagcttgga ccgtgatgac aatggtcttt
     7021 tgactcttgc tggcggtaaa atcacagact accgtaagat ggctgaagga gctatggagc
     7081 gcgtggttga catcctcaaa gcagaatttg accgtagctt taaattgatc aattctaaaa
     7141 cttaccctgt ttcaggtgga gaattgaacc cagcaaatgt ggattcagaa atcgaagcct
     7201 ttgcgcaact tggagtatca cgtggtttgg ataacaagga agctcactat ctggcaaatc
     7261 tttacggttc aaatgcaccg aaagtctttg cacttgctca cagcttggaa caagcgccag
     7321 gtcttagctt ggcagacacc ttgtcccttc actatgcaat gcgcaatgag ttgactctta
     7381 gcccagttga cttccttctt cgtcgtacca atcacatgct ctttatgcgt gatagcttgg
     7441 atagtatcgt tgagccaatt ttggatgaaa tgggacgatt ctatgactgg acagaagaag
     7501 aaaaagcaac ttaccgtgct gatgtcgaag cagctctcgc taacaacgat ttagcagaat
     7561 taaaaaatta agaaaaaata aaagaggtgg agggcagcat tccttgtcgc ccgtcccttc
     7621 tttttaatgg agacagaaag atgatgaatg aattatttgg agaatttcta gggactttaa
     7681 tcctgattct tctaggaaat ggtgttgttg caggtgtggt tcttcctaaa accaagagca
     7741 atagctcagg ttggattgtg attactatgg gttgggggat tgcagttgcg gttgcagtct
     7801 ttgtatctgg caagctcagt ccagcttatt taaacccagc tgtgaccatc ggtgtggcct
     7861 taaaaggtgg tttgccttgg gcttccgttt tgccttatat cttagcccag ttcgcagggg
     7921 ccatgctggg tcagattttg gtttggttgc aattcaaacc tcactatgag gcagaagaaa
     7981 atgcaggcaa tatcctggca accttcagta ctggaccagc catcaaggat actgtatcaa
     8041 acttgattag cgaaatcctt ggaacctttg ttttggtgtt gacaatcttt gctttgggtc
     8101 tttacgattt tcaggcaggt atcggaacct ttgcagtggg aactttgatt gtcggtatag
     8161 gtctatcact aggtgggaca acaggttatg ccttgaaccc agctcgtgac cttggacctc
     8221 gtatcatgca cagcatcttg ccaattccaa acaagggaga cggagactgg tcttacgctt
     8281 ggattcctgt tgtaggccct gttatcggag cagccttggc agtgtttgta ttctcacttt
     8341 tctagtttat actcttcgaa aatcaaattc aaaccacgtc agcgtcgcct taccgtactc
     8401 aagtacagct tgcggctagc ttcctagttt gctctttgat tttcattgag tattagaaaa
     8461 caattatgtt gatagagctt gggcaagagc ccaatttcag caaaaaatga agtaaatctt
     8521 ctcataataa aacgcatcat atcaagcacg aaaattccac gaggtcaact acagtcagaa
     8581 agctgaacaa caagccaaaa cgcccaaaaa aggcggcaaa aagcaagcac ctgcaagcaa
     8641 cgtgccgaaa tggtcaaatc ctgattatgt caacgaatta gacccaaaaa tcgttgatat
     8701 gctagtagaa tttcacaagt cacaaggcac tttggaaact cccgaggcgc aagcagaaat
     8761 cgcccaaaaa cgtgaagaaa tcgagcaaag gagagctgag cttgaggata aaaaacaaga
     8821 gcttttgaac cgcttgaaca aatagagttt cgcaagtatt atgcttacaa attacttgag
     8881 caattaacta aaatataaac cctgccttta tatctaggca gggtttatat tttagaaatt
     8941 cacgtaggtt gttacggttt ttacataccc agtatagttt gagtttctat agtattcagt
     9001 gataaacttc cattttcttt gagcaacatg gatataagta cttgttatgt agtatggata
     9061 tgggctttgt gaatccaagt aagactgata agcttgtata ccaaaatatg ctccaccaat
     9121 tattgcaccc catggacccc ccaataaagc acctatccta ccaatcatat aactgattcc
     9181 agcaccagtc atgaagttag cgaatgtgtt agcttgttta ttcccatgta ttgtgttgac
     9241 gtaattccaa acattaggat cgtatgatct aaaagatata tttaggtcga tttcattctt
     9301 ttgataagcc atataaaatg ccccattgat atagacgccg tcagcacgtc gttcaatagt
     9361 gtctacactt ccatctggat tgacaacctc aagaacttca tcgcttaaaa tatttacttg
     9421 cgtatctccg aaccgcactg atgagccatt ctcaaactga gcctcaccag atacaacttt
     9481 agagtttgcc gataagctat catcagcaaa aacaacaagc gacggggaaa tgctagacat
     9541 acagaaaaca gacataacta gcaaacacat gcatttaaac atcttagaca taacggaaac
     9601 tcctttgtat ttttgatttt ttttaacttt tattatacaa taaaaccaaa taaaaagaaa
     9661 gcggtaacaa tatgcttaat gcgaaaattt tttatatatt tttatgtttg atcgttatcg
     9721 aaactacagg cttgttgttg ttgaaaagag gtctcgaaat gggttattta gacacagaag
     9781 ctattatcct cgcagttttt tcatttgctt tttacaacct atgttcattc gcttgggtct
     9841 gctctacaat aaaaaacaat aaaaaataaa tagacgtatt ttcaaaaaaa aaaaaaaaac
     9901 gcatatttat cttagcaaaa cgacgattta aatcgtcgtt tttttgtagt acgacgggca
     9961 tgtcgtatat ctgaggtgta agtcctcggt gggcacctgc taccggtgaa cccaatagcg
    10021 attcccaagc ctgactatcg tgaggtagca gggagaggaa gggatagcga aatcgtggct
    10081 ctacgaacag gaacgtgata gtaaggcgta tatagcggat aaggaggctt caaactctaa
    10141 agtccaaaaa ggtagtcgta acctatatgt gtaaatcacg agagtaattg aattcggact
    10201 aaggtttgtg tgaaaaagat aaatctttct agagtctaaa gactctgcgt cagatttcct
    10261 attttcactg taacctttta acgtcctcat atcttgtata aacgaggaaa gatgtacgac
    10321 ttatcccgtg aggtttcatg agcgctgaaa gcgtagtaac aacgaatcat gagaagtaag
    10381 ccgagcccat agtagtgagg aaacttccgt aatggaagtg gagcgaaggg gtgaatactc
    10441 aaacagtctg gggagagact gtttgaggtc tgtcgctaga aagagaaaac gacagatcga
    10501 agtaatccta cttcacttgt gtctgtaaaa tgagtggtct gatagaactg gactttgaca
    10561 cgtaatgact agatgaaggt tcaccaatat aagatgtccc tcaggcacca aaacaagaaa
    10621 ggaatacgca catgtcaaaa ctgctagata agatattatc acgcgaaaat atgctggaag
    10681 cctacaatca agtaaaatcc aataaaggct cagctgggat tgatggaatg actatcgaag
    10741 agatggataa ttatctcaga caaaactggc gcttgactaa ggaactgata aaacagagaa
    10801 aatataagcc tcaaccagtt cttagagttg agatacctaa accagacgga ggcatccgtc
    10861 aactaggaat tccaacagtt atggatagaa tgattcaaca ggccattgtc caagtcatga
    10921 gccccatttg tgaaccccat ttctcagata cgagttatgg tttcagacca aataggtcat
    10981 gtgaaaaagc catcatgaag ctcttagaat acttaaatga cggctatgag tggatagtgg
    11041 atatagacct agagaaattt ttcgatacag ttcctcaaga tagattgatg tccttagtac
    11101 ataacattat cgaagacgga gatacggaat ccttgattcg taagtatctt cattcaggtg
    11161 ttatcattaa tggtcaacgt tataaaacac tagttggtac accacaggga ggaaatttat
    11221 ctcctctctt atccaatatc atgcttaatg aattggacaa ggaattagaa aagaggggac
    11281 ttcgatttgt gcgctacgca gatgattgtg tgattacggt cggaagcgag gcagcctcta
    11341 agcgtgtgat gtattcagtc agtcgtttta ttgagaaacg gctaggtttg aaagtaaaca
    11401 tgaccaagac taagattacc agaccaagag agttgaaata tctaggtttt gggttctgga
    11461 aatcatcaga tggttggaaa agccgtccac atcaagatag tgttcggaga tttaagttta
    11521 aattgaagaa actaacacag aggaaatgga gtatagacct aacaagacgt attgagcaac
    11581 tgaatttgtc tattcgagga tggataaact atttctcatt gggaaatatg aaaagtatag
    11641 tcgccagcat agatgagcgc ttgcgtactc gcctacgagt gattatctgg aagcaatgga
    11701 agaagaaatc gagacgatta tggggattgc ttaagttagg aattcctaaa tggataacag
    11761 ataaggtatc tggctggggc gaccattatc aattagtagc tcagaagtcg gtacttaaac
    11821 gtgctatatc aaaaccagtc ctggaaaaac gtggactggt ttcgtgtttg gattattacc
    11881 ttgaacgaca tgcgttaaaa gttagttgaa ccgccgtatg ccgaacggca cgtacggtgg
    11941 tgtgagaggg gctagagatt atcccctact cgattaactc ccctgaaatt tattttaatt
    12001 atgcaaattt cacgtatttt tgatgctgag acgacgatcc tgggaacttt tcagatattt
    12061 tttgactatc taaatctatc attagaaaag cttagagcgc caaaggattt gagcgttttt
    12121 ctgattttta agactttttc cagtctcttt ttcgattgaa gatgtaatta ttctactaac
    12181 taactaactt cttagtacta gccaacaacg ataatcataa ttcctcctaa aattaggaat
    12241 aataaaggca atagtttttg ttttttcatg taaaaaacct cacttttgtt ttctgctatt
    12301 ttatgctaaa atattaaaaa tcaaatttaa ttccaaagtt tgtaactaaa gggggagcgc
    12361 tacatgtcta attcatttgt caagttgtta gtctctcaat tatttgcaaa tttagcagat
    12421 attttcttta gagtaacaat cattgctaac atatacatta tttcaaaatc agtaattgcc
    12481 acatcactag ttcctatctt aataggaata tcctcttttg ttgcgagtct tttagttccg
    12541 ttggttacta aaaggttagc gctaaatagg gttttatctt tatctcaatt tggaaagact
    12601 atattattgg cgatactggt aggaatgttt accgtaatgc aatctgtagc gcctttggtg
    12661 acctatctat ttgttgttgc aatttccata ctagatggtt ttgcagcacc cgtttcctat
    12721 gctattgtgc cacgctatgc gaccgatttg ggtaaggcta attcagcctt atcaatgact
    12781 ggtgaagctg ttcaattgat aggttgggga ttaggtggac tcttgtttgc aacaattggt
    12841 ctgttaccta ccacgtttat cattttagtc ttgtatatca tttctagctt tctgatgtta
    12901 tttcttccta acgctgaagt ggaggtgtta gagtcagaaa ctaatcttga aattttgctc
    12961 aaaggttgga agttagttgc tagaaatcct agattaagac tttttgtatc agcaaattta
    13021 ttggaaattt tttcaaatac gatttgggtt tcttccatta tacttgtttt tgtaacggag
    13081 ttattaaata aaacggaaag ttactgggga tattctaata cagcatactc tattggtatt
    13141 ataattagtg gcttaattgc ttttaggcta tctgaaaagt tccttgctgc taaatgggaa
    13201 agtattcttt ttcctcttgt agctatggca atagtgacac tgactattct atattttcca
    13261 aacgcacaga tgtttttatt attttcagct ttagttggta tgttatcgca actaaaagaa
    13321 gttcctgaaa gtgtatttct tcaggaaaca gtagaggaaa atcatttagt taatgtctat
    13381 tccgttcttg aagtgatttc tacattagca ttttcagtat ttgttttgct aatgagctat
    13441 attactgaga gttttggtat tagcatcagt ttttggctat cagccatttg tctgatgata
    13501 gaagctattt taatttatat cagacgagat tattttaaat gagagagtgg ttaaaaagtc
    13561 tttaaaaatc agaaaagcgc atagtatcag gtgttgaaaa aacttgatat tgtgtgtttt
    13621 attatgggaa gatttacttc attgtctcct gaaatggagt tttgaagcag gatcactcta
    13681 tttgaaaata tacatagaaa aaccgcataa tccttaaaat aagctgaaat ctattggaaa
    13741 catacggtaa acaattgaaa agatactata taaagtggta caatggtaga aaaataagct
    13801 agtaagaatc actcttattt tagcgaaaaa ttacagaaat gaatggtttt tcttgatgaa
    13861 acagagaaaa gaattgtacc tctttcttgg tcggacagcc ttgtattttc ttatctttct
    13921 agggctgctt tacttcttta gctatcttgg tcagggtcaa ggaagcttta tctataatga
    13981 attttaactt gaaggagaat ccctattgtg tcaaataaac caatagcaga tatgattgaa
    14041 accattgagc attttgctca gacacagcct agctatcctg tctataatgt tttggggcag
    14101 gaacacactt atggcgattt aaaggctgat tcggatagtt tggctgcagt cattgaccaa
    14161 ctaggcttgc ctgagaagtc tcctgtggtt gtttttggtg gccaagaata tgaaatgttg
    14221 gcaacctttg tagcgctgac taagtcaggt catgcctaca ttccaattga tagccattcg
    14281 gccttggagc gagtttcagc tattttagaa gtagcagagc caagcttgat tattgccatt
    14341 tcagcctttc ccttggagca ggtttctaca ccaatgataa atctagctca ggttcaagaa
    14401 gcctttgccc aagggaataa ctatgaaatc acgcatccag tcaagggaga tgataattac
    14461 tacattatct ttacttctgg tacgactggt aagcctaagg gagtgcagat ttcacatgat
    14521 aatctcctca gctttacaaa ctggatgatt acggataagg aatttgcgac accgagtcgt
    14581 ccgcaaatgc tggcacagcc accttattct tttgacttgt ctgtcatgta ttgggcaccg
    14641 accttggcac ttggtggtac gcttttcact cttccttcag tcatcactca ggactttaag
    14701 caactctttg cggctatctt ttcattgcca atcgctatct ggacatcaac accatccttt
    14761 gcagatatgg ccatgttgtc tgaatacttc aacagtgaga aaatgcctgg aatcacgcat
    14821 ttctactttg atggtgaaga attgacggtc aaaacagctc aaaaactgcg cgagcgtttc
    14881 ccaaatgccc gtatcatcaa tgcttacggc ccaacagaag cgacagtagc tctgtcagca
    14941 gttgccgtga cagacgagat gttagcgact ctcaaacgcc taccaatcgg ctataccaag
    15001 gctgattctc caacctttat cattgacgag gaaggaaata aactgccaaa tggtgaacag
    15061 ggagaaatca ttgtttctgg gccagctgtt tcaaaaggtt atatgaaaaa tcctgaaaaa
    15121 acagcagaag ccttctttga gtttgaaggt ctgccagcct atcacacagg cgatgtggga
    15181 accatgacag atgagggctt gcttctctac ggcggacgca tggacttcca gattaagttt
    15241 aacggttacc gcattgagtt agaagatgtc tctcaaaacc tcaacaagtc tcgctttatc
    15301 gaatctgctg tcgcagtacc gcgctataac aaggaccaca aggtacaaaa tctattggct
    15361 tatgtcatct taaaagacgg tgttcgtgag cagtttgagc gagatatcga tattaccaag
    15421 gccatcaagg aagacctgac agacatcatg atgtcctata tgatgccatc taaattcctt
    15481 taccgagaca gtttgccact aactccaaat ggaaagattg acatcaaagg attgattaac
    15541 gaggtgaata agagatgatg gagttttttc aacagcttcc tcatttagag ccatatggca
    15601 atcctcagta ttttgtttat gtgattgctg caaccttgcc catctttata ggcctctttt
    15661 tcaagaaacg ctttgcctgg tatgaagtgt tggtaagtct cttctttatt gtcaccatgt
    15721 tggtgggtgg aaagaccaat caactagctg ccttgggtat ttacctttgc tgggaaatat
    15781 tgctcctgct tttctacaag cattatcgaa aaagcaagga tggcaagtgg gtcttctact
    15841 tagttagttt tctgtcccta cttccgatta tctttgtcaa ggtgcaacca gctatcaatg
    15901 gaacgcagtc tttgcttggg ttcttgggaa tttcttacct gacctttcgt tcggttggga
    15961 ttatcatcga gctgagagat ggagtgatta aggattttac cctctgggaa ttcctccgtt
    16021 tccttctctt catgccaact ttctcgagtg gtccaatcga tcgctttaag cgatttaatg
    16081 aaaattatca ggctattcct gagcgagatg agttgataga tatgctggat gaatctgtcc
    16141 gctatatcat gtggggcttt ttgtataagt ttatcctagc tcatgtttta ggagagacct
    16201 tactacctcc tctgaagaat ttagccttgc agtcaggtgg cttctttaat ctctatgcct
    16261 tggcagttat gtatactttt ggtctggagc tcttctttga ctttgcaggt tattctatgt
    16321 ttgctttggc catctcaaac ttgatgggaa tccgtagccc tatcaacttt aacaagccct
    16381 ttttatcaag ggatttaaag gagttttgga atcgctggca tatgagtctg tccttctggt
    16441 tccgtgactt tgtctttatg cgaatggtga tggtgttaac cagaaagaaa gtctttaaaa
    16501 atcgtaatgt aacctcaagc atggcctaca ttgtaaatat gctgattatg ggattttggc
    16561 atggtgtgac ctggtactat atcgcctatg gactctttca tgtactaggc ttggtcatca
    16621 atgatgcctg ggttcgcaag aaaaaaacgc tcaataagga acggaaaaaa gcagggaagg
    16681 ctgccctacc tgagaatcgc tggattcagt tgcttggcat ggttgtcact ttccatgttg
    16741 tcatgttgtc attcttaatc ttttctggat tcttgaataa tctatggttt aaaaaataaa
    16801 aggaaataaa atatggatat caaatcagaa gttattgaaa ttattgatga gttgtttatg
    16861 gaagatgttt ctgacatgat ggatgaagat ctttttgatg caggtgtctt ggatagtatg
    16921 ggaacggttg agttgattgt ggagattgag aaccgttttg acattcgtgt ccctgtaaca
    16981 gagtttggtc gtgacgactg gaatacagct aataaaatca tagctggtat tgtggagcta
    17041 caaaatgctt aaacgcttat ggatgatctt cggaccggtc ttgatcgctg gtttgttggt
    17101 ttttctgctc attttctttt atcctactga gatgcatcat aatctaggag ctgaaaagcg
    17161 ttcagcagtg gctactacta tcgatagttt taaggagcga agtcaaaaag tcagagcact
    17221 atctgatcca aatgtgcgtt ttattccctt ctttggctct agtgaatggc ttcgttttga
    17281 cggtgctcat cctgcggtat tagctgagaa atacaatcgt tcctaccgtc cttatctttt
    17341 aggacagggg ggagctgcat cgcttaacca atattttgga atgcaacaga tgttaccaca
    17401 gctggagaat aaacaagttg tgtatgttat ctcacctcag tggttcagta aaaatggcta
    17461 tgatccagca gccttccagc agtattttaa tggagaccag ttgactagtt ttctgaaaca
    17521 tcaatctggg gatcaggcta gtcaatatgc agcgactcgc ttactgcaac agttcccaaa
    17581 cgtagctatg aaggacctgg ttcagaagtt ggcaagtaaa gaagaattgt cgacagcaga
    17641 caatgaaatg attgaattat tggctcgttt taatgaacgc caagcctcct tttttggtca
    17701 gttttcggtt agaggctatg ttaactacga taagcatgta gctaagtatt taaaaatctt
    17761 gccagaccag ttttcttatc aagcaataga agatgttgtc aaagcagatg ctgaaaaaaa
    17821 tacttccaat aatgagatgg gaatggaaaa ttatttctat aatgagcaga tcaagaagga
    17881 tttgaagaaa ttaaaggatt ctcagaaaag ctttacctat ctcaagtcgc cagagtataa
    17941 tgacttgcag ttggttttaa cacagttttc taaatctaag gtaaacccga tttttatcat
    18001 tccacctgtt aataaaaaat ggatggacta tgctggtcta cgagaggata tgtaccaaca
    18061 aacggtgcag aagattcgct accagttaga aagtcaaggg tttaccaata tagcagattt
    18121 ttctaaggac ggcggggagc ctttctttat gaaggacacc attcaccttg gttggttggg
    18181 ttggttggct tttgacaagg cagttgatcc tttcctatcc aatcccacac cagctccgac
    18241 ttaccatctg aatgagcgct ttttcagcaa agattgggcg acttatgatg gagatgtcaa
    18301 agaatttcaa tagataatat aatatagaag agttcaagaa tgaaactagt tttcacgttt
    18361 tttcttgact ctttttttgc ttgtgatata attaactggt aaacaaaatg tcaaagaata
    18421 gtatttttct cttaaaaaag agaagtctaa aaggaaagga gtcagatatg agacagctag
    18481 caaaggatat caatgctttt ttgaatgagg tgattttgca ggcggaaaat cagcatgaaa
    18541 tcctaatagg tcattgcact agcgaggtgg ccctgaccaa tactcaggag catatcctta
    18601 tgctcttgtc agaggaatct ttaacaaatt cagaattggc ccgtcgtctc aatgtcagtc
    18661 aggcggcagt taccaaggcc attaagtctt tggtcaagga agggatgttg gaaacatcta
    18721 aagattctaa agatgcgcgt gtgatttttt atcagttgac tgacttggct cgtccaatcg
    18781 ctgaggagca ccaccatcac catgagcata cacttttaac ctatgaacaa gtggcgactc
    18841 agtttactcc aaatgaacaa aaagtgattc agcggttttt gactgcttta gtaggagaaa
    18901 tcaaataatg agatatatta cggtagagga tttgtccttc tattatgata aggagcctgt
    18961 tcttgaacat atcaattata gtgttgatag tggggaattt gttaccttga ctggggaaaa
    19021 tggagcggct aagacgacgc tcatcaaggc cagtcttgga attctgcaac cacgcattgg
    19081 aaaggtggct atttcaaaga caaatacgca aggtaagaaa ttgagaatag cctatcttcc
    19141 tcaacaaatt gccagtttta atgctggttt tccaagtacg gtctatgaat ttgtcaagtc
    19201 gggtcgctat ccgagaaaag gctggttccg tcgtttgaat gctcatgatg aggagcatat
    19261 caaggctagt ctggactcag ttggcatgtg ggaacatcga gacaaacgct tggggtctct
    19321 atctggggga caaaagcagc gagcggtaat tgcgcgtatg tttgcttctg accctgatgt
    19381 gtttatccta gacgagccga caacggggat ggatgcagga agtaaaaatg aattttacga
    19441 actcatgcac cacagcgccc atcatcatgg caaggctgtt ttgatgatta cccatgaccc
    19501 tgaagaagtt aaggattatg cggatcgcaa tattcatcta gtccgtaacc aagactcgcc
    19561 atggcgttgt ttcaacgttc atgagaatgg ccaggaggtg ggccatgctt agtttattat
    19621 cttacgactt tatacaacgc gcctttttgg cggttattgc tatgagtctt ttctcaccgg
    19681 tattgggaac cttcctcatc ttgcgtcgtc agagtttgat gagtgatacc cttagccacg
    19741 tctcactttc aggtgtagcc tttggtctgg ttttggggat ttctccaact gtttctacta
    19801 ttgccattgt cttgattgcg gcggtctttc tggagtatct ccgtacagtt tacaagagct
    19861 ttatggaaat cgggacagct atcctcatgt caacaggtct ggctgtttct ctgattgtca
    19921 tgagtaaggg taaaagctcg agttcaatga gtttggacca atatctcttt ggttcgatcg
    19981 tgactatcag tgaagaacag gtcatttccc tctttgtcat tgcggcggtt gttttgattt
    20041 tgacctttct ctttcttcgt cctatgtata tcttaacttt tgacgaagat acggcctttg
    20101 tggatggctt gccagttcgt accatgtcca ttctttttaa catggtgaca ggggtggcta
    20161 ttgcccttat gattcctgca gcaggagctc ttctggtatc gaccattatg gtcttgccag
    20221 ctagtattgc cctgcgtctg gggaaaaact ttaaatcggt tatgctgctt gccagtgcga
    20281 ttggcttttt gggaatggta gcaggacttt atatttccta ctatgcagaa acacctgcaa
    20341 gtgcaagtat taccattatt tttgtaactg tctttatact aatcagttta gtaagacgtt
    20401 ttatcaaata ggagacgaat gtgaaaaaaa ttagcttatt actagccagt ctatgtgcct
    20461 tgtttttagt ggcttgttcc aatcaaaaac aggcagatgg taaactcaat atcgtgacaa
    20521 ccttttaccc tgtctatgaa tttaccaagc aagtcgcagg agatacggct aatgtagaac
    20581 tcctaatcgg tgctgggaca gaacctcatg aatacgaacc atctgccaag gcagttgcca
    20641 aaatccaaga tgcagatacc ttcgtttatg aaaatgaaaa catggaaaca tgggtaccta
    20701 aattgctaga taccttggat aagaaaaaag tgaaaaccat caaggcgaca ggcgatatgt
    20761 tgctcttgcc aggtggcaag gaagaagagg gagaccatga ccatggagaa gaaggtcatc
    20821 accatgagtt tgacccccat gtttggttat caccagttcg tgccattaaa ctagtagagc
    20881 acatccgcga cagcttgtca gcagattatc ctgataaaaa agagaccttt gagaagaatg
    20941 cagctgccta tatcgaaaaa ttgcaagcct tggataaggc ttacgcagaa ggcttgtctc
    21001 aagcaaaaca aaagagcttt gtgactcaac acgcagcctt taactatctt gccttggact
    21061 atggactcaa acaagtcgca atctcaggac tttctccaga tgcagagcca tcagctgctc
    21121 gcttggcaga attgacagag tatgtcaaga aaaataaaat cgcctatatc tattttgaag
    21181 aaaatgcctc acaagccctt gctaacacac tttcaaaaga agcaggtgtc aaaactgatg
    21241 tcctcaatcc tttagaaagt ctgacagaag aggacaccaa ggctggagaa aactacattt
    21301 ccgtgatgga gaaaaacctc aaggctttga aacaaacaac agaccaagaa ggcccagcaa
    21361 tcgaacctga aaaggcagag gataccaaga cagtcccaaa tggttacttc gaggatgcag
    21421 ctgtcaagga ccgcaccttg agtgactatg caggtaactg gcaatcagtt tatcctttcc
    21481 ttgaagacgg cacgtttgac caagtctttg actacaaggc taagttgact ggtaagatga
    21541 cccaggctga gtacaaggct tactatacaa aaggctatca gacagatgtg actaagatta
    21601 acattactga taatactatg gaatttgttc aaggtggaca aagcaagaaa tacacttaca
    21661 agtatgtcgg taagaaaatt ttgacttaca agaaaggcaa tcgtggcgtg cgtttcctct
    21721 ttgaagccac agatgctgac gctggacaat tcaagtatgt tcagtttagt gaccacaatg
    21781 ttgccccagt taaggcagaa catttccata tcttctttgg aggcacaagc caagaagccc
    21841 tctttgaaga aatggacaac tggccaacct actacccaga taacctatct ggccaagaaa
    21901 tcgcccaaga aatgttggcg cattgatgag aaatcgacta gttcataaga gctagtcggt
    21961 tttttgatac tagatggcta acttcattat agacctttca gaactttaaa caataaataa
    22021 aactcttgaa atatctgggc ttatatgcta gaatgtagtt agtgtataag gttcattaga
    22081 acaccaaagc ccctaactag tgccatagtt aagggctttt ttgacgtatc ttagcgattt
    22141 aacgggtgtt tataggctag tcattcggtc tacttcttac catctagcca tttgcagacg
    22201 aaatacaaga taattccagc tataacatct gctataatag taagagcaag ctctggtata
    22261 aggctcatta gtttcacctc ctctcacgaa ccgtaaggaa cgtaatcggt aaccgatgac
    22321 aaaaatagta tatcacacta cattcaggtc atcaacctta cttaatgctt gaactattgt
    22381 agattatatg ctagaatgta gttaattgat aaggttcatt agaacaccaa agcccctaac
    22441 tatggccgta gttaagggct tttttgacgt atcttagcga tttaacgggt gtttataggc
    22501 tagtcagtcg gtctactcct tactatctag ccatctgcag acgaaataca agataattcc
    22561 agctataaca tccgctataa tagtaagagc gagctctatg ataaggctca ttagtttcac
    22621 ctcctctcac gaacccatag gaacgtaatc ggtaaccgat gacaaaaata gtataccaca
    22681 atacatttag atcatcaagg tcacttaatt cttgaaatat cagatctaag agaaaaatct
    22741 ttaaaatcag aaaaacgcat aatatcaggt gtgcaaaaac ttgatactat gcgttttatt
    22801 gtgggaaggt ttactccatt ttctcctgaa attgagtttt tgtccagcct ctgtttttag
    22861 ggttgctaag aaaataatgt catgtggtga atatttgtaa atcagtcagc agacagaacg
    22921 atactcttcg aaaatctctt cacatcatgt cagcttcgtc tttccgtata tatgtgactg
    22981 acttcatcag ttctatctac aacctcaaaa cagtgtttcg agctgacttg atcaattttc
    23041 aaatctgtac tttgagcaag ctgagactag cttcctattt gattttcatt gaatatcaga
    23101 aacccattct ccatcaaata attcgactgc gtctaataat ttttgatctg gcacggtgtc
    23161 tgaaataaag gttgtgtatt tggagagggg attaatttta aaaaatccag tcttgtaaaa
    23221 tttagaacta tcaatcagta agatggtttc atgggctttg tcaataatat tcttttttga
    23281 aatagcttgg ctgagagaag cttcataaac atattggtca tcaatacctc ttgctgaaca
    23341 aaatgctaaa tcgatattaa aatgatctaa taaagaattt tccttatcat agttgaccac
    23401 ggaacaggat tgatgtttga cctcgccaga tgtgataaag attttggagc tatctttaac
    23461 agtttcagat agggtttgtg cagtatgtaa accatttgta aaaataatca aattatcaag
    23521 ttcagaaaga tagggacaga gttcgtagac agtagtacta gaatctagat agatacacat
    23581 accagaccga ataaagtctt tagcgagact agcgattagt cttttttgcc tagtactttc
    23641 tccttcacgt atttgatgag aaagttcaat tgtgttcata gaggacaggg tcacgtatcc
    23701 gtgctttctt ttgataagac cttgattttc taagaaaatt aaatcacgac gtaaggtact
    23761 tgtgctggag aaagtgattt ctgccagctc ttttacggca attctttttt tctttttgat
    23821 aatttcaatc aattcaagta cacgttcatc ttttatcata agctcctcct aatttatcat
    23881 ttcaactata ttatagcaca aattggagga atttgaatta tttttatgaa tattgggtta
    23941 acatttgaac attattcaag taagcgttca catattgaaa aaataaaacg tggggattat
    24001 aataaagtta atcaaggacg aagagagaag aaaaatggaa gcggttttag caatagattt
    24061 aggtgcgact tctggaagag caatcgttgg ttacctttct gaaaataaac tagtaatgga
    24121 agaaataaat cgcttttcta atctacctat tagagtaaaa gggcatttat cttgggatat
    24181 tgactttcta ctagctaaaa ttcttgaaag tatccgcttg gctaatacta gttacaagat
    24241 tttatctatc ggtattgaca catggggagt tgattttgga ctgattgata atgaaggtaa
    24301 gctgttatta caacctgttc attatcgtga tgaaagaaca aagggagtgt taaaggaaat
    24361 atctgaaatg actgaattag aaaaactgta ttcagagaca ggaaatcaga ttatggagat
    24421 aaataccttg tttcaactct ttaaggcacg tcaagaatct cctgactctt tctataagac
    24481 caataagatt cttttaatgc cagatttgtt taattatctc ttgacaggta agtttgctac
    24541 agaaaaaagc attgcttcaa caactcaatt atttgatcct aggagtcaaa attggaatca
    24601 gaatatctta aaactatttg aattggattc atctttactt cctgaaattg tttcagaggg
    24661 aaatgttctt ggaaggataa aagaggagta tggtttaggc gatattcctg ttgtgaatgt
    24721 ttgtagtcat gatacagcaa gcgcgattgt ctcagtacct aagacagaag gtagtttatt
    24781 tatttcatca ggtacttggt ctttggttgg agtggaactt acttcaccga ttcttactac
    24841 cgaatccttc agttatggat ttacaaatga agtcggtaaa gatggagtga ttacatttct
    24901 gaagaattgt acagggttgt ggatcataga ggaactaaga cgttcatttg aacgaagagg
    24961 gaaagcctat tcttttgatg atattaggac aatggtggag aaagaaaaag aaaatcttcc
    25021 tctgattgat actgaatcaa ctgaatttgc aacagaatct gatatgcaca agactttgac
    25081 agaatatcta gcttatcatc atgaaactag agagtggaca gatggacaac tatttaagat
    25141 tgtttatgaa agcctagctg aaacgtatag gaaagcgata gagttactag aagaactaac
    25201 tcataaggtt tataagagga tatatgtgat tggaggaggt gctagagcca gttactttaa
    25261 ccaaatgatt gctgatagaa ctggtaaaga ggttcttaca ggtttgactg aggctacagc
    25321 tgtggggaat attgttgtgc agctcatagc tatgggacaa ttaaaaggga tggaagaggc
    25381 tcaccatgtt attgaggagt ttctacaatt agagagttat tactcccaaa agaattaaaa
    25441 agattgagag tttgtaaatt tgcctccctc ccccttctta gcttttgtgc aggaaggggg
    25501 gataattggt gaattgaaaa atatttagtg ttttgatatg aggaggacaa ggatgtcaga
    25561 tgtaaaacaa gaattaatta aatatggtaa gaagctagta gaaacagatt tgacgaaagg
    25621 aacaggtggg aatctcagcg ttttcgatcg tgaaaaacaa ttgatggcaa ttaccccgtc
    25681 gggtattgat ttctttgaaa tcaaagaatc cgatattgta gtgatggata ttaatggaaa
    25741 tgttgtagag ggagaacgct tgccatctag cgaatggtat atgcatttga ttcaatatca
    25801 aactcgtgat gatatcgatg caattatcca tgctcataca acttatgcaa cagtattagc
    25861 ttgtctcaga gaaccacttc cagcgagtca ttatatgatt gcagtggcag ggaaagatgt
    25921 tcgggtagct gagtatgcaa catatggcac gaaagaattg gctgtgaatg cagctaaagc
    25981 aatggaaggt cgtagagcag ttttactagc gaatcatgga attttagcag gtgcacaaaa
    26041 tttattgaat gcatttaata ttgttgaaga agttgaatat tgtgcaaaaa tttattgttt
    26101 agctaagaat tttggagagc cagtagttct tcctgatgag gagatggaat tgatggcaga
    26161 aaaatttaaa acatacggtc agagaaaata gggaggatat taatgttaaa acatataccg
    26221 aaaaatattt ctccagattt attgaagact ttaatggaaa tgggacatgg agatgaaata
    26281 gtattagctg acgcgaatta cccttccgtc tcatgtgcga ataaattaat tcgttgtgat
    26341 ggtgtaaata ttccagaatt attagattcc attctgtatt taatgccatt agatagttac
    26401 gtcgatagtt caattcagtt tatgaacgtt gtttcgggtg atgatattcc taagatatgg
    26461 ggtacctata gacagatgat tgaaggtcat ggtacagatc ttaaaacgat tacttatctt
    26521 agaagagaag acttttatga acgtagtaag aaagcttatg ctattgttgc tacaggagaa
    26581 acttcacttt atgctaatat tatccttaag aaaggagtag ttgttgaaag agaaaatgtt
    26641 taatagagga attttagttg ccagtcatgg taattttgct agcggagctc tcatgaccgc
    26701 agaaatgttt gttggtgaga caacaaatga tagagttagg acattaggtt tgatgcctgg
    26761 agagaatatt gtagagtttg agcattattt taaaaatcaa gtggatgaac tgttagactc
    26821 aaatcaagag gttatcgttt tgactgactt gattggagga agtcctaata atgttgcttt
    26881 gtcacggttt ttaaatttgg attcagttga tattgtaaca gggtttaata tccctctcct
    26941 agtggaatta atatcaaatt atgattcaaa aatcaattta gaagaaattg ttcacaatgc
    27001 tcaaaatagt ttgtttaatg ttaaacaaca acttaacgta gaggaggaag aagatttatg
    27061 tctatagagt ttgttcgtat tgatgaccgt ctggtacatg gtcaagttgt cactacgtgg
    27121 ctaaaaaagt atgatattga gcaagttatc attgttaatg atcgcatctc agaagataaa
    27181 atacgacaat ctattttaaa gatttctgca ccggtaggtt taaaaattgt tttctttagt
    27241 gtaaaacggt ttgtggaagt tttaaactct gtgccaataa aaaagagaac aatgctgata
    27301 tatacaaatc caaaagatgt gtatgattct attgaaggaa atttaaaatt ggagtacctc
    27361 aatgtaggac agatgagtaa aacggaggaa aatgaaaagg taacgggagg tgtagctcta
    27421 ggtgaagaag acaaatatta ttttaagaaa atagttgata agggaacgag agttgaaatt
    27481 caaatggttc ctaatgataa agttacaatg ttggaaaaat ttttataaaa ataatttaag
    27541 gaggtacagt atatgctatt cacacaagca ttactggtga cattagttgg gattattgcc
    27601 actattgact ataatggacc gttatttatg attcaccgtc cgttagttac aagtgcaatg
    27661 gttggcttag tcttaggaga tttcacccaa ggtgttctta ttggttcagc tcttgaatta
    27721 acttggctcg gtgtaacagg tattggaggt tacactccac cagatactat ttcaggtgcg
    27781 attattggta ctgcatttgg tattttgtct ggtcaaggag aaactgctgg tatcgctata
    27841 gcagttccaa ttgcagttgc tacccaacag ttggatgttc ttgcaaaaac tttagatgtt
    27901 tattttgtga aaaaagctga taatgatgct aaaaacggag attattcaaa gatcggtttt
    27961 tatcattatt caagtttggt tttaatcacg ttatttaaaa ttgtaccaat tttcctagct
    28021 attatgcttg gaggggaata tgtggcagac ttgtttgcta aggttccacc aatcgttatg
    28081 cagggactta actctgcagg tgctttacta ccttcaattg gttttggtat gcttttaaat
    28141 atgatgctca agaaaaatat gtgggtattc ttgttgattg gattcatttg ttctgtgtat
    28201 ggaggaatgt caaccattgg gatctcacta gttggtattg cggtagcata cttctacgat
    28261 atgattggaa gtaaaccaca agaaacaact tcaagtagtg atgttgagga ggatcttgat
    28321 ctatgatgaa taataaagta actaaagttg aacttaaaaa agttttcaaa cgaagtttta
    28381 tgtatggttc ttcatggaac tatgagagaa tgcagaacct aggttttcta tatacaattc
    28441 ttccagtatt gaaaaaacta tacccagaca aagattcagc ttctcctgca atgaaacgtc
    28501 accttgagtt tttcaatact catcaaacag cggcaccatt tattcttgga gttacttccg
    28561 ctatggaaga acaagaagga aatgaaggtg cagcttcaat tactggtatt aaagttggct
    28621 tgatggggcc actggctggt ctaggagata gtttgttctg gctgacacta gttcctatct
    28681 gttttagtat tggtgcgtct tattctaaag acggcggtgc tttaggtatc tttatcgcct
    28741 taatattgtt taatattatt aatattcctg ttaaatattt cggtttgaaa tatgggtata
    28801 ctaagggttc tagtcttatc caagaaaata atacaaaagg aacattgaat cgcgttacga
    28861 gtatggcgac agcattaggg ctagtactag tgggtggttt gattccatca atggttggta
    28921 ttaattttgg attagaattt aagcaggggg aacttgttat ttctgttcaa gaaatgatta
    28981 caaaattaat tccaggattt atccctatgg ctttgacttt attaatgtgt aaattaatta
    29041 gaaaaggaaa gaatccggtt gtactaatct ttagtgttat ggctattgga gttatcctag
    29101 ttgttttagg aattttgaag tagtagaaag tgtggaggtg gtatttggga tatcacctcc
    29161 attttggaag agaggtaaag agtgaaatta tggtataaga aagctgccgc aaattggaat
    29221 gaagccttgc cgattgggaa cggtcattta ggtggtatga tttatggttc agctacaaaa
    29281 gaatgtattc aactaaacga tgagactatt tggtatagag gaaagtcaga tagaaataat
    29341 ccagactcac tattgcatct taaaaaaatt cgggaatatc ttttagatgg agaaattcag
    29401 aaagccgaag aattgataaa gttaacaatg tttgctaccc caagagatca aagccactat
    29461 gaattacttg gggaacttta cattgagcat atagatattc agtcttgtgc tctttcattg
    29521 tatgaaagag agctagattt agatacagct atttctaatg ttgtgtttga gcctaatagt
    29581 tgtaatttac aaataaaaag agaatatttt acgagtttta ataagaatat tttatgttgc
    29641 cgtatagtgt catcagttca aaacacatta aatttaaaca ttaatttggg tagaaataaa
    29701 cggtttaatg acgaagtatc taaactggat tcaagtacaa ttttaatgtc ggcctctgct
    29761 ggaggtagaa aaggtgttca gtttaaagta gtatgtcatt ctaaggttac ggatggtgaa
    29821 gtaagtgtat tgggagagac aatagttatt cggaatgcta cagaggtatt tctttatctc
    29881 aaatcaatga cggattattg gggaaatata gatatttctt ctcttcaggg agaatttagt
    29941 agtattgatt actttacaga aaaagatgaa catgtaaaaa aatatcagga gcaatttaat
    30001 agagttgatt ttaaactaga ctatagtaaa ggttgtctta gcattccaac gaatctactt
    30061 cttgaaaaca ctaaaaagta tagtaactac ttgactaact tgttatttca ttatggaaga
    30121 tatctgttaa tatcgtctag tcaaccgaat ggtttacctg ccaatcttca aggaatatgg
    30181 tgtgatgaat taaatccaat ttggggttct aaatatacga ttaatattaa tactcaaatg
    30241 aattattgga tggtaggtcc atgtgattta ccagaagtag aatatccatt atttgatatg
    30301 ctcgaaagaa tgagagaacc gggaagacta accgctaaga aaatgtatgg agctagaggt
    30361 tttacagcac atcataatac ggatggtttt ggcgatacgg ctccccaatc tcatgccatg
    30421 ggggctgcaa tttgggtatt aactattcca tggttatgta ctcatatttg ggaacactat
    30481 ttatatttcc aagatgagcg tattcttacg gaacattttg aaatgataaa agaagcattt
    30541 cttttctttg aagattattt atttgaggtg gatggctact tgatgacagg tccaagtgtc
    30601 tcaccggaaa ataaatatcg cttaaaaaat ggtattgaag gaaatgcttg tctatcatct
    30661 acaattgata atcaaatttt aagatatttt tgtgattcat gcattggaat tgcaaaacaa
    30721 ttaggagaca attcggattt tattaatcgt gtgaaggagt taaaaaagaa attacctaga
    30781 acaaaaatag gtagtaatgg gcaaatacaa gaatggctag aggattatga agaagtagag
    30841 cctggtcata gacacatttc acctctattt gggctttatc cttataatga gattgatatt
    30901 cataaaactc cggaattagc agaagcagct aaaatcacta tcaatagaag attatcaaac
    30961 gctaactttt tatcttcaca ggatagggag caagcgatta ataattggtt agtaagtggt
    31021 ttgcatgcta gtacacaaac aggttggagt gctgcatggc tgattcattt ttttgcgaga
    31081 ctatatcaag gtgaacctgc ttataaccag attaatggtt tgttaaataa tgcgactctt
    31141 ggcaatttat ttcttgacca tccaccattt caaattgatg gtaatttagg tttggtgagt
    31201 ggaatttgtg aattattagt acagagccat cataattggt tatcactaat tccagcttta
    31261 ccttctgctt ggtcagaagg agaagtgaaa ggtttcagag taagaggagg atataaggta
    31321 tcgtttgctt ggaaaaatgg ggatataaca ttcctaaaat tggaaggagg aaacaaagat
    31381 caaaaagtaa gagtaagaat atatggcaaa aatactgatg tacaaaatat tgaattggta
    31441 tttaattcag aaaaaattat tgagttaaat ttttaggtat aagtcatgaa taaagaaaaa
    31501 ataaaaagaa aattaatcac aatactgttt gtatgtattg ggatgttatg ttttggattg
    31561 ttagcaggag ttaaggctga taatcgtgtt caaatgagaa cgacgattaa taatgaatcg
    31621 ccattgttgc tttctccgtt gtatggcaat gataatggta acggattatg gtgggggaac
    31681 acattgaagg gagcatggga agctattcct gaagatgtaa agccatatgc agcgattgaa
    31741 cttcatcctg caaaagtctg taaaccaaca agttgtattc cacgagatac gaaagaattg
    31801 agagaatggt atgtcaagat gttggaggaa gctcaaagtc taaacattcc agttttcttg
    31861 gttattatgt cggctggaga gcgtaataca gttcctccag agtggttaga tgaacaattc
    31921 caaaagtata gtgtgttaaa aggtgtttta aatattgaga attattggat ttacaataac
    31981 cagttagctc cgcatagtgc taaatatttg gaagtttgtg ccaaatatgg agcgcatttt
    32041 atctggcatg atcatgaaaa atggttctgg gaaactatta tgaatgatcc gacattcttt
    32101 gaagcgagtc aaaaatatca taaaaatttg gtgttggcaa ctaaaaatac gccaataaga
    32161 gatgatgcgg gtacagattc tatcgttagt ggattttggt tgagtggctt atgtgataac
    32221 tggggctcat caacagatac atggaaatgg tgggaaaaac attatacaaa cacatttgaa
    32281 actggaagag ctagggatat gagatcctat gcatcggaac cagaatcaat gattgctatg
    32341 gaaatgatga atgtatatac tgggggaggc acagtttata attttgaatg tgccgcgtat
    32401 acatttatga caaatgatgt accaactcca gcatttacta aaggtattat tcctttcttt
    32461 agacatgcta tacaaaatcc agctccaagt aaggaagaag ttataaatag aacaaaagct
    32521 gtattttgga atggagaagg taggattagt tcattaaacg gattttatca aggactttat
    32581 tcgaatgatg aaacaatgcc tttatataat aatgggagat atcatattct tcctgtaata
    32641 catgagaaaa ttgataagga aaagatttca tctatattcc ctaatgcaaa aattttgact
    32701 aaaaatagtg aggaattgtc tagtaaagtc aactatttaa actcgcttta tccaaaactt
    32761 tatgaaggag atgggtatgc tcagcgtgta ggtaattcct ggtatattta taatagtaat
    32821 gctaatatca ataaaaatca gcaagtaatg ttgcctatgt atactaataa tacaaagtcg
    32881 ttatcgttag atttgacgcc acatacttac gctgttgtta aagaaaatcc aaataattta
    32941 catattttat tgaataatta caggacagat aagacagcta tgtgggcatt atcaggaaat
    33001 tttgatgcat caaaaagttg gaagaaagaa gaattagagt tagcgaactg gataagcaaa
    33061 aattattcca tcaatcctgt agataatgac tttaggacaa caacacttac attaaaaggg
    33121 catactggtc ataaacctca gataaatata agtggcgata aaaatcatta tacttataca
    33181 gaaaattggg atgagaatac ccatgtttat accattacgg ttaatcataa tggaatggta
    33241 gagatgtcta taaatactga ggggacaggt ccagtctctt tcccaacacc agataaattt
    33301 aatgatggta atttgaatat agcatatgca aaactaacaa cacaaagttc tgtagattac
    33361 aatggagacc ctaatagagc tgtggatggt aacagaaatg gtaattttaa ctctggttcg
    33421 gtaacacaca ctagggcaga taatccctct tggtgggaag tcgatttgaa aaaaatggat
    33481 aaagttgggc ttgttaaaat ttataatcgc acagatgctg agactcaacg tctatctgat
    33541 tttgatgtga ttctatatga caataataaa aacgaagttg ctaagaaaca tgttaataat
    33601 ttgtcgggtg aatctgttag tctagatttc aaagaaaaag gagcaaggta tattaaagtt
    33661 aaattactaa cgagtggagt gcctttgagt ttagcagaag tagaggtttt tagagaatca
    33721 gatggtaagc aatctgaaga ggatatagat aaaataacag aagataaagt agtctctaca
    33781 aataaggtag ctactcaaag ttcaaccaat tatgagggtg tagctgcttt agcagttgat
    33841 ggtaataaag atggagatta cggacatcat tcggtgactc atactaaggc agattctaac
    33901 gcttggtggc aggtcgatct gggagaagag tttacggttt ctaaagttga tatttataat
    33961 agaacagatg ccgaacctca gcgtttatct aattttgatg ttatttttct atcttcatca
    34021 ggagaagaag tttttagaag acattttgat aaagtagttg atggtttgtt atctttaaaa
    34081 gtaccttctg taggggctaa gctagtcaaa atagaattaa aatcagcagc tattccgtta
    34141 agtttagcgg aagttgaagt ctatggttca aagagaactc cgaagaaact ttctaatatt
    34201 gcattaacca aagaaactcg acagagttca acggattaca atggtttttc tcgtctagca
    34261 gttgatggaa ataaaaacgg agattatagt catcattcag tgactcatac caaagaagat
    34321 tctccttcat ggtgggagat agatttagca caaaccgaag aattagaaaa gttaattatt
    34381 tataatagaa cagatgctga aattcagaga ttatcaaatt ttgatattat tatatatgat
    34441 tcaaatgatt atgaagtttt tacacaacat attgacagtt tagaaagcaa taatctatcc
    34501 atagacttaa aaggactgaa gggaaaaaag gttagaattt ctttgagaaa cgcaggaatt
    34561 cctttaagtt tagcagaggt agaggtttat acttataagt aattttaaaa attatcaccc
    34621 aggctaccgt aaatataatg gcgatggtag tatgaaagaa acagaaaaat aagaggaaaa
    34681 tagtatgatt caacatccac gtattgggat tcgtccgact attgatggtc gtcgtcaagg
    34741 tgtacgcgaa tcacttgaag tacaaacaat gaacatggct aaaagtgtgg cagatttgat
    34801 ttcaagcaca ttgaaatatc cagatgggga acctgtggaa tgtgtgattt ctctatctac
    34861 cattggtcgt gttccagagg ctgcagcttc ccatgagttg ttcaaaaaat caaatgtttg
    34921 cgcaacaatt acagttacac catgctggtg ttatggtagt gaaactatgg atatgtctcc
    34981 agatattcct catgctattt ggggatttaa tgggacagaa cgcccaggag ctgtctatct
    35041 tgcagctgta ctagcttcac atgctcaaaa agggattcca gcctttggga tttatggtag
    35101 agacgtccag gaagctagtg atacagctat tccagaagat gtcaaagaaa aacttttacg
    35161 ttatgcgcgg gcagctcttg caactggctt gatgagagac actgcttacc tatcaatggg
    35221 tagtgtttcg atggggattg gtggttctat tgtaaatccg gatttcttcc aagaatactt
    35281 aggaatgcga aatgaatcgg tagatatgac ggagttcaca cgccgtatgg accgtggtat
    35341 ttacgaccct gaagagttcg aacgtgcgct caaatgggtg aaagaaaacg taaaagaagg
    35401 attcgaccat aaccgtgaag accttgtttt aagccgtgaa gaaaaagata gacaatggga
    35461 atttgttatt aagatgttca tgattggacg tgacttaatg gttggtaacc caagacttgc
    35521 tgaacttggt tttgaggaag aagcagttgg tcaccatgct ttagtagctg gtttccaagg
    35581 tcaacgtcag tggacagacc attttccaaa tggagatttt atggaaactt tcctcaatac
    35641 tcagtttgac tggaatggta ttcgaaaacc atttgtattt gcgacagaga atgattcact
    35701 aaatggtgtg tctatgctct ttaattatct attaacaaat actccacaaa tctttgctga
    35761 tgtgcgtact tattggagtc cagaggctgt tgaacgtgta acaggatata ctttagaggg
    35821 tcgtgctgca gctggcttct tacatctaat caactctggt tcttgtacat tagatggtac
    35881 aggtcaagct actcgagatg gcaaacctgt tatgaaacca ttctgggagt tggaagaaag
    35941 tgaagtgcag gctatgcttg aaaatacaga cttcccacca gcaaaccgcg aatacttccg
    36001 tggaggagga ttctcaactc gtttcttgac gaagggggat atgccagtaa caatggtacg
    36061 tctcaatctt ctaaaagggg ttggtccagt gctacaaatt gcagaaggtt atacacttga
    36121 acttcctgaa gatgttcacc atactttaga taatcgtaca gatccaggat ggccaactac
    36181 ttggtttgct ccacgtttga caggaaaagg tgctttcaag tctgtctatg acgtcatgaa
    36241 taattgggga gctaatcacg gagccataac atatggacac attggagcag acttgattac
    36301 cttggcttct atgttgagaa ttcctgtcaa tatgcataat gtacccgaag aagatatctt
    36361 tagacctaaa aattggtcct tatttggaac agaagatcta gaatcagcag actatcgtgc
    36421 atgtcagttg ttgggaccac tacataaata aaacttgttt atataggagg taaacttacg
    36481 tccctcctat ccttttaaaa atatttgtta aaaaattcac aaataattga aaacgaatac
    36541 aaaaagtaat ataatgatgt taaatagata gcgcaaaggc gcaggaggaa aattatatgg
    36601 ctacatttta tgttccggca gtcaacctta ttggtaaagg tgttgtaaat gaagtgggtc
    36661 cttatatcaa ggaacttggc tataaaaagg cacttttggt gacagataag tttatcgaag
    36721 gaagtgacat tttgcctaag gttttaaaac cgctagatac agaaggaatc gaatatgtca
    36781 tctttagcga tgtagagcca aaccctactt gtaagaatgt cacagatggg gtagctgctt
    36841 tgcaagaaca tggctgtgac tttatcatca gtcttggtgg gggttctcca caggatgcag
    36901 ctagttgtat ttctatcatg gctataaatg gtggaaaacc acaggattat gaagggcttc
    36961 ataagtctgc taaaaaaggc ttgccagttg tggcgatcaa tacaacggca ggaacttctg
    37021 cagaaatcac tattaactat gtgattactg atgaagaacg caaggttaag atggtaatgg
    37081 ttgacaagaa tagccttgct cttatctctg ttaatgaccc ggaactcatg ctttccaaac
    37141 ctaaaggcct gactgctgct actggtatgg atgctctgac tcacgctgtt gaagctttgg
    37201 taacacctgg tgcttatgat gtaaccaaga aactgtctat tggtgctatt gagcttatca
    37261 aggaatatct tcctcgtgct gtagaaaatg gacatgatat tgaagcgcgt gaaggtatgg
    37321 tcaatgccat cttccttggt ggtatgagct ttaataatgc tggtcttggt tatgttcact
    37381 caatggctca ccaactcggt gcagtatata atttgccaca tggtgtttgc tgtgccatgt
    37441 tgctaccagt tatagaacgt gaaaatgcta aacgtgtacc agaagctttc cgcaatgttg
    37501 ccaaagcctt gggacttcat gtagaaggta aatcagatca agaatgtgcc gattatgcga
    37561 ttactgagat tgagaagctt tctgagacag taggtattcc taagaaactg actgaacttg
    37621 gtattgaaga aaaagatttc gactttgaat acctttctaa gaatgccttg attgatgcct
    37681 gcgcaccagg aaatccattt atgccaacct tagaagaaac gattgccttt tataaagagt
    37741 tattttagca aggattagac gacaatagga caattcacct ctaattattg acatttcagg
    37801 aaaaatcgca gtaaaaacgg cacagccttc tttaaatgtg ctataatact agaaaaatac
    37861 ttgtggaggt tccattatgg caatattttt catgattttt ctgattgttt gtgtgctcct
    37921 attggtgata gtcacactga gtacagttta tgtggttcgt cagcagtcgg tggcgattat
    37981 tgaacgcttt gggaaatacc aaaaggttgc taatagcggt attcatattc gcttgccttt
    38041 tgggattgac tcgattgcag cacggattca gttgcgcttg ttgcaaagtg atattgtggt
    38101 tgagactaag accaaggaca atgtgttcgt tatgatgaat gtagcgactc agtaccgtgt
    38161 caacgagcag agcgtgacag atgcttacta taaactcata cgtccagaat ctcagattaa
    38221 atcttatatc gaagatgctc ttcgctcttc tgttccaaaa ttaaccttgg atgaattgtt
    38281 tgagaaaaaa gatgagattg cccttgaagt tcaacaccaa gtagcagaag aaatgaccac
    38341 ttacggctac attatcgtga aaaccttgat taccaaagtc gaaccggatg cagaagttaa
    38401 gcaatccatg aatgaaatca atgcggcgca acgtaagcgg gtcgcagcac aagaattggc
    38461 ggaagctgac aagattaaaa ttgtcactgc agctgaagcc gaagcagaaa aagaccgcct
    38521 tcatggtgtg gggattgccc aacaacgtaa ggcgattgtg gatggattgg cagagtctat
    38581 caccgaactc aaggaagcca atgttggcat gacagaagaa caaatcatgt ctatcctctt
    38641 gaccaaccag tatttggata ccttgaatac ctttgcctct aaaggaaatc aaaccatctt
    38701 tttaccaaat actccaaatg gtgtggatga tatccgaaca caaatcttgt cagcccttcg
    38761 cgctgagaag aaataataga ctaatactct tcgaaaatct cttcaaacca cgtcagcgtc
    38821 gccttgccgt atagatgtta ctgacttcgt cagttctatc tgcaacctca aaacagtgtt
    38881 ttgagctgac ttcgtcagtc ttatctacta cctcaaaacg gtgttttgag caacctgtgg
    38941 ctagtttcct agtttgctct ttgattttca ttgagtacaa agagcttggc aacaggctct
    39001 ttttgtattg cgtttattaa gaaaaacaaa aaacatcgat atacatttaa catacattta
    39061 actaaaaatt attgtatgtt atctccctta attaggaatg ataagggaat aactagaaag
    39121 atttgtgaat acaaactatt tctgatatac aaaatataca gtaataatga atgatgggag
    39181 atgggatgaa agaatttcaa tttgagagaa agcagcgttt ttctttgagg ccatatgcaa
    39241 taggagcttg ttcggtcttg ctaggaacga gtttattttt tgatggtatg ggtgatcagc
    39301 ctgtacagga tacagaaacg agttcagcac taattttaag tcattatttg gatgagcagg
    39361 atttatctga aaagctgaaa tctgagttgc aatggtttga attagaaaac aagcttttga
    39421 acttatggga gcattaggtt actatgaagg atttgttcct tatgtttcaa atcaatacaa
    39481 aaaccaagct gaagaagaag gcaaaccgct atctgataaa tatattttcg aaaaaatctt
    39541 aggaaaaaca tatgcagcct tcaaaaaaga tcaaattaat gagcgtgtcg agaaattagg
    39601 taagttaaaa cctattacaa taaattacaa cggaaaatca gaagtaattg atagtaaaga
    39661 aaaattacaa gagcttatga ataaagccgt taaagacgaa gtggctcaaa tatagtaaaa
    39721 tgaaataaga acaggacaaa tcgatttcta aaaatgtttt agaagtagag gtgtactatt
    39781 ctagtttcaa tatactataa aatcaggcaa taccacggct aaaaagtttg agtttatcga
    39841 aacaccagtg caaacactga aaaaagcgat ttataaagct catctcaaag attcagatga
    39901 ctttaaacca gaaacatcca cataaatctt tttgaaagct gtttgaagct ttgtccttgc
    39961 tttcttagtt catagatctg aactttatct tcataattca atttcataat aaaacacccc
    40021 aaaagttaga tttattctgt ctaacttttg gggtgcagtt cattaatcgg tcgatttttt
    40081 tttgtagaaa cagttgcctg attaagttgc aagtcgatag acggcttcgg cataaatatc
    40141 catagcacgg tacaaatctt ctagaacggc acattcattt gcctgatgtt ctgtctgctt
    40201 cgctcctggg aataaggcgc cgaaggctac acaatttggc atggtgcgag caaaagtggc
    40261 accaccggat gaaatagcag gactgttatc gccagtcttt tcttggtaga tttgcatcag
    40321 tgtgctaacg agtttactgt cttttgcgac gtatagaggc gctagatagt caaactcttc
    40381 gtagcggagt tggtagtttt gtgcacatct tgtaagcaac tctactagtt cttccttgtc
    40441 agctaagaca ggagtccgaa tgtcaatacg gatttcagaa cgttcatgat tgatcatgag
    40501 acctgcgaca ttaaaggata ggtgaccaga aggttcatct gctatatcac caaagatttg
    40561 tcttcctgtg ccgtcttgac ctgcttgtgt tgcaagaaaa ctgagagcag ggtgttcttg
    40621 gagaggagca agaatggtag ctagtcggat gacagcattg ataccttgac tagcatcctt
    40681 agcatgcttt ggcactccga gaaccgttac ggtttgttca gtggtttggt aatcataacc
    40741 agcttctttg agaccgttac aaacctgttc atagaggaga ccttggtagt tggccttgtc
    40801 tggtacaacg ttaaaggcgc ctcctacttc aagctctagt tgatccgatc ccgggccatg
    40861 aagtttgacc tgtagaagcc ctttttcagc ataggtcaga ggaaaagatg agtcaggtgc
    40921 aaagcccata ctggcctgtt cttcgatggt attgtagcgt gccatgcagc gccagaggtt
    40981 ttcctcatcg gtaccaaaga taaagcgtac gcgctttttg aactgaatac cttggtccag
    41041 caagcttttt actgcataga gagctgcgag cgaagggcct ttatcatctt ggacaccacg
    41101 tccgaatacc cagccgtctt tgatagttgc ttcaaatggc ggtgtctgcc aatctgcttc
    41161 atcacctgat ggaacaacat ccaaatgaca gagaatggcc agaagctctg ctccctgacc
    41221 gatttctgca tatccgtaat aacctttagg gtcaagatag gtagtgaaac ctatgtctcg
    41281 acaaatctct aaagtttttt ctaggacatc ttggattgct tgtccaaaag gtgttccatt
    41341 ttctccttca ttgagtactg aaggatagga aatcaaggtt tttaatgata taagaaattc
    41401 atctttaact tgatttgtta tatctatttt catggaatca cctcactcac tattttatag
    41461 gaatggaagg aaggttccaa ggagaagaag ggcgatggtc actacaataa tagcgactac
    41521 gagtttgccc atgaatttcc accaagtacc aatgttgata cgtccaagtg caagagctcc
    41581 catcacaata ccagatgttg gtgcaatcaa gttcaagaca cctgaagcag attgataagc
    41641 agtgataatc aagctaggac ggacatttac aaattctcca agtggagcca tgatacccat
    41701 agttgcgctg gcaagaccag atgaagatgg gatcaagaat gacataggta gatagaagat
    41761 ataagttaca acgataaaga cttgtgaaga tagaccgctc aagccttctt taccccagtt
    41821 gaggattgta tcggtaatca taccgtcgtt catgataact tgaataccac gagcaatcgc
    41881 tacgatcaag gcaacactga gcaagtcagc agcaccattc atgaaggaag agataatctt
    41941 atcttcttta agaccataaa taacaccaat caggataccc ataaaggcaa agagcattgc
    42001 gccttctggg aagtaccaag tacctagtgc agaagtagat gaaccgacaa tattaccaat
    42061 aactggaaga ccagtcaacc aagcattaaa gtcatcaaaa atggtaacgc caaggtctgt
    42121 ccatggaatg aagctcaata ccatcaagat gaatgtcaac acaaataaaa agagaactga
    42181 tttttgtttg ctgctaagtg tagattctac agatgaagat tcttctacgt taaagtgttt
    42241 caaatcttct ttgcgagtac tataaaccag tgacttagtc ggatcttttt gaatcttatc
    42301 cgcataacgg taaacaaacc aagtactaag agcagtcaag gtaacccaga aaatcagacg
    42361 aagtacgata ccgtcccctg taccaactcc cgcagtcgct gaagcaatac ctgtcgcaaa
    42421 tggattcaga gtagatgcca aacagccgat ttgagaaccg agcaaaataa ttgcaacacc
    42481 agtcaggcta tcaaaaccaa cggccatcat aactggcaca aggagtggat agaaggccat
    42541 tgtttcttcc cccataccat aagttgtacc accgagggca aacaaaggca tcagtaccaa
    42601 aattaacatt ttttcgcggc ccttatactt cttcacgata gaggcaatcc ctacgtcaag
    42661 agcaccagtt ttgttgacaa tgccaaggaa accaccaacc ataaggatga agaaggctac
    42721 atcaatcgct gcgctcgttt ctttaatgag cgaaccttcc tctggatgag tacctagcat
    42781 agcccgaatc ggtgccatga ggacatccca aatcccttgt ggattttgag gctgagcctc
    42841 gtaaatacct tctataaagg cccccgcagg gataaaccaa gttagcactg ccataatagc
    42901 aatgattatc aataatacgg tgtaagatga aggcatctta aacccttttt tagctttttc
    42961 actcatttgt ttttcctcct gatgtctaac aaagtgtaaa aaaccaacta ctttcttaaa
    43021 taaacattca agaaaacgct ttctatttat atttgtttta ccatattaaa gtctaaaaaa
    43081 ctaatatctt ttgaaatatt gtacatgttt tcaagaataa tttctaattt gatataaaga
    43141 tactagaaat agaatacatc ttattagtaa aacaacttat cctttttcaa taattgttcc
    43201 gctttcagat tcaatcaagg cgcctagatt ttcaagggaa gtaataactg cttttccttc
    43261 tggacgacca ttgacaaaag cgatagctgc ttctactttt ggaagcatgc tacctggtgc
    43321 aaactgatct tgtttgatat attcttccag ctgggcaaca ttcacatgtt ccaatttttc
    43381 ctggtttggc ttgttgtagt taacaaatac ataatctaca cctgtcaaaa cgatgaagag
    43441 gtctgcctca accaattctg ccaaacgttg ggaagcgaag tctttatcaa taaccgcttc
    43501 gacaccagtc aaatgtccat tgttttcttt gacgacggga ataccgccac cacctgcagc
    43561 tacgacgact tgaccattat ttaaaagagt acggatggtt tcaatttctt tgatgtcaac
    43621 aggttttggt gaggcaacga ccttacgcca gccacggcca gcatcttcct tgaaagtcgc
    43681 tccgcttttt tcggcttctg cttttgcttc ttcttctgaa tagaaaggac cgattggttt
    43741 actcaagtta acaaaagctg gatcattttt atctacgaca acttgcgtta caacagaggc
    43801 aacatttttt tcgatgcctt catccaagag agcattttgc aaagcatttt tcaaccagaa
    43861 accgatgcta ccttctgtca tagcgacaag tgagtcgagt gggaaggcag ggttcttttc
    43921 agagtctgat gccaaatgtt ggagcaagag attcccaact tgaggtccat taccgtgagt
    43981 gataatcaga tcatctccat ttttaatcaa ttttacaaga tgcttagctg tttcaactaa
    44041 agcttcttgt tgagcctttg ctgatgggtc agaagaaaga atcgcatttc ctcccaaagc
    44101 tactacaatt ttacgatttg ccataaattc tcctttatca cactcaatag aatgcgttta
    44161 gatttcaatt taatgatttt tcacatattt tataagaaat aatagattac cattacataa
    44221 aagaggaccg gactaaagct attagtcgca gccctcatag ctgttggtag acggtttatt
    44281 atctaaaatt atactttagg aatataaagg ttaccaagtg tagcagccat aacagctttg
    44341 atagtgtgca tacggttttc tgcttgatcg aagtggcgag cgtacttgct gcggaagact
    44401 tcgtctgtta cttccatttc ttctacacca aatttttcag caacgtcttt accataaaca
    44461 gtgtgagcat cgtggaatgc tggcaagcag tgtaggaaga tcaagttttc attgcctgct
    44521 tttttaacta agtccatatt gacttggtaa ggtttaagaa gagctacacg ttctgcgaat
    44581 ttgtcttctt cacccattga tacccaaacg tctgtgtaaa gaacgtctgc atctttaact
    44641 gcttcatcag catcttcagt gatgagaaca tgtgcgccac tttctttagc aaatccttct
    44701 gccaattcaa cgatttcttt ttctgggaag agttcttttg gtgagaagat gtgaacattg
    44761 acaccaagga tagcacctgt tacgagcaag ctgttggcaa cgttgttacg tccatcacca
    44821 cagtatacca atgtcaagcc ttccaagcga ccgaagtttt cttgaacagt caagtagtca
    44881 gcgagcattt gagttgggtg ccattcgtca gttagaccgt tccatactgg aacgcctgag
    44941 aattctgcca attcttcaac catacgttgg ctgaatccgc ggaattcaat cccgtcaaac
    45001 atacgtccca atactttagc agtatcttca gtagattctt ttttacccaa ctgaatatca
    45061 tttgctccga ggtattctgg gtgagcacca aggtcgatag ccgcagttgt aaaggctgca
    45121 cgagtacgag tagatgtttt ttcaaatagg agagcgatat tcttgccagc aaggtagtgg
    45181 tgttgaatat tgcgtttttt caaatctttc aagtgagctg aaagaccaat aaggtattct
    45241 aactctgcac gggtaaagtc tttttctgct aagaagctgc gtccttggaa tactgaattt
    45301 gtcattttat tatttcctct ttctattttt tacattttct attgacgaat accgaacagc
    45361 gattacactt cttcacgttc aaatggcata gacatacaac gaggtccacc acggccccga
    45421 accaattcac ttccgcgaat cttaatcaag cgaagcccgt attcttccaa aatcttattg
    45481 gtcacggtat tgcggtcata aacaactacc acaccaggtg cgatggtcaa agtgttagaa
    45541 ccgtcgttcc attgttcacg cgcagctgct acgatattgc caccaccgca acgaatcaaa
    45601 tgaacttttt ctacaccaag gttttgagca agaagttcag ctaagtcacc tttctcttca
    45661 acgattttaa gtttttcgtt ttcgtaagta actgagtaaa cgtgaaggtc gccttcgatt
    45721 tctgggtgaa tagtgaactt gtcatagtct accatagtga agacagtatc caagtgcatg
    45781 aatttacggt tgttagcaaa ttcaaaggcc aaaactttct tgaagccaac atttttcttg
    45841 aagatgttga ccaaaagttt ttcgatagaa gctgcgtctg tacgttgaga gatacctact
    45901 gcaaggacgt ctttagaaag aactaactcg tctccacctt cgatacgcgt atcttcttca
    45961 cggttgtaga ccaaatccac ttttccgcca tagattgggt ggtatttgaa gatatactta
    46021 ccgtagagtg tttcacggtt acgagtgtct gcaaacatgt ggttaagcga tacggcgttt
    46081 ccaattgttg caaatgggtc gcgagtgaaa tagaggtttg gcatcgggtc aattgcaaat
    46141 ggataatctg attcaactaa gtcagttaga tctttagatt cgtcaggaat ttctggcaat
    46201 tcaacttttt gaatcccagc cattgttttt tcaaccaatt cttggttgtc cttgatgccg
    46261 tgaagcaatt cacgaatagc aaccttggtt tgacgatcac ggatgttggc ttcgtctaag
    46321 tattcctcga taaattgatc gcggatttct ggagaggtca atgattcagc agcgagttgt
    46381 tctaggtaga gaacctcaat tccttcatcg cgaagagctt gggcaaatgc atcatgttct
    46441 ttttgagcat cttccaagaa aggaatatca tcaaaaagaa gcctttcaag atagtccggc
    46501 aacaagtttt ctaactcctt gcctggacgg tgtaacataa cttttttcag tttcccaatt
    46561 tctgagaaga cctgaattgg atgtgaagac atgtattttc ctccttaaaa attttgactt
    46621 ggcaccattt tgggtaccgc ttacatgtat gttataacgc aattcctagg taatgtcaat
    46681 ggctataatt tctcggaaaa taatataaat tataatattt agcacaattt tatg
//
LOCUS       DAVKJD010000008        44717 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKJD010000008 DAVKJD010000000
VERSION     DAVKJD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.008"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(722..1972)
                     /gene="ilvA"
                     /locus_tag="VW157_000457"
     CDS             complement(722..1972)
                     /gene="ilvA"
                     /locus_tag="VW157_000457"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9551811.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(2022..2162)
                     /locus_tag="VW157_000458"
                     /pseudo
     CDS             complement(2022..2162)
                     /locus_tag="VW157_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
     gene            complement(2347..2583)
                     /locus_tag="VW157_000459"
     CDS             complement(2347..2583)
                     /locus_tag="VW157_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551812.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     SREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(2690..3712)
                     /gene="ilvC"
                     /locus_tag="VW157_000460"
     CDS             complement(2690..3712)
                     /gene="ilvC"
                     /locus_tag="VW157_000460"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9551813.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYIVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(3778..4254)
                     /gene="ilvN"
                     /locus_tag="VW157_000461"
     CDS             complement(3778..4254)
                     /gene="ilvN"
                     /locus_tag="VW157_000461"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9551814.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGVTEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEERAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(4247..5947)
                     /locus_tag="VW157_000462"
     CDS             complement(4247..5947)
                     /locus_tag="VW157_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9551815.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVKFHA
                     "
     gene            complement(6148..7815)
                     /locus_tag="VW157_000463"
     CDS             complement(6148..7815)
                     /locus_tag="VW157_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9551816.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(7818..8183)
                     /locus_tag="VW157_000464"
     CDS             complement(7818..8183)
                     /locus_tag="VW157_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9551817.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(8340..8528)
                     /locus_tag="VW157_000465"
     CDS             complement(8340..8528)
                     /locus_tag="VW157_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9551818.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(8644..8820)
                     /locus_tag="VW157_000466"
     CDS             complement(8644..8820)
                     /locus_tag="VW157_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9551819.1"
                     /translation="MDLDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLSIN
                     NGMWRAENVTQDLI"
     gene            complement(8931..9129)
                     /locus_tag="VW157_000467"
                     /pseudo
     CDS             complement(8931..9129)
                     /locus_tag="VW157_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
     gene            complement(9398..10942)
                     /locus_tag="VW157_000468"
     CDS             complement(9398..10942)
                     /locus_tag="VW157_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9551820.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            11155..11457
                     /gene="gatC"
                     /locus_tag="VW157_000469"
     CDS             11155..11457
                     /gene="gatC"
                     /locus_tag="VW157_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9551821.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            11457..12923
                     /gene="gatA"
                     /locus_tag="VW157_000470"
     CDS             11457..12923
                     /gene="gatA"
                     /locus_tag="VW157_000470"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9551822.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            12923..14365
                     /gene="gatB"
                     /locus_tag="VW157_000471"
     CDS             12923..14365
                     /gene="gatB"
                     /locus_tag="VW157_000471"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9551823.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNIHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            14489..15532
                     /locus_tag="VW157_000472"
     CDS             14489..15532
                     /locus_tag="VW157_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEU9551824.1"
                     /translation="MATMKAARWHAAKDVRIQEIEVPEVLPHQVKVAVKFTGICGTDL
                     HEFLDGPIFIPTDEHVYSGQKAPVTLGHEFSGEIIEVGSDVTRVKVGDRVAVEPILAK
                     NNLVGDYNLDPNLNFVGLAADGGFAKYCVLDGDLVHVIPDSLSYEQAALTEPAAVAVY
                     AVRQSSLKAGDTAVVFGLGPIGLLIVEALRAAGASKIYAVELSPERQAKAEELGAIVV
                     RPEEGETAVEAIHRLTNGGVDVSYEVTGVPVVLGQALAAVHKAGECMVVSIWEREANI
                     NPNEFAIQEKTLKGIIAYRHIFPKVLELMEQGYFSAEKLVTKKIKLENIVEEGFIELT
                     QDKSQIKILVEPE"
     gene            complement(15665..15742)
                     /locus_tag="VW157_000473"
                     /pseudo
     CDS             complement(15665..15742)
                     /locus_tag="VW157_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            15910..16470
                     /gene="efp"
                     /locus_tag="VW157_000474"
     CDS             15910..16470
                     /gene="efp"
                     /locus_tag="VW157_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9551825.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            16492..16881
                     /locus_tag="VW157_000475"
     CDS             16492..16881
                     /locus_tag="VW157_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9551826.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            16874..17296
                     /gene="nusB"
                     /locus_tag="VW157_000476"
     CDS             16874..17296
                     /gene="nusB"
                     /locus_tag="VW157_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9551827.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(17346..17582)
                     /locus_tag="VW157_000477"
     CDS             complement(17346..17582)
                     /locus_tag="VW157_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9551828.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(17595..17984)
                     /locus_tag="VW157_000478"
     CDS             complement(17595..17984)
                     /locus_tag="VW157_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551829.1"
                     /translation="MYVTIILGLGADVYSLILTMGLVGSFLLLIWRLKKKKMLFIFEK
                     KSWNWSFVFYLFATYVVYQILGNFWARYAHLINHRNIHDEYFTVLFSNGQPTFLSTIL
                     SFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(18078..18263)
                     /gene="briC"
                     /locus_tag="VW157_000479"
     CDS             complement(18078..18263)
                     /gene="briC"
                     /locus_tag="VW157_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU9551830.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(18422..19189)
                     /locus_tag="VW157_000480"
     CDS             complement(18422..19189)
                     /locus_tag="VW157_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9551831.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(19186..20052)
                     /locus_tag="VW157_000481"
     CDS             complement(19186..20052)
                     /locus_tag="VW157_000481"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9551832.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNVGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(20089..21456)
                     /gene="accC"
                     /locus_tag="VW157_000482"
     CDS             complement(20089..21456)
                     /gene="accC"
                     /locus_tag="VW157_000482"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9551833.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(21468..21890)
                     /gene="fabZ"
                     /locus_tag="VW157_000483"
     CDS             complement(21468..21890)
                     /gene="fabZ"
                     /locus_tag="VW157_000483"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9551834.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(21887..22372)
                     /locus_tag="VW157_000484"
     CDS             complement(21887..22372)
                     /locus_tag="VW157_000484"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9551835.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(22375..23610)
                     /gene="fabF"
                     /locus_tag="VW157_000485"
     CDS             complement(22375..23610)
                     /gene="fabF"
                     /locus_tag="VW157_000485"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9551836.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(23632..24363)
                     /gene="fabG"
                     /locus_tag="VW157_000486"
     CDS             complement(23632..24363)
                     /gene="fabG"
                     /locus_tag="VW157_000486"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9551837.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(24397..25317)
                     /gene="fabD"
                     /locus_tag="VW157_000487"
     CDS             complement(24397..25317)
                     /gene="fabD"
                     /locus_tag="VW157_000487"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9551838.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(25310..26284)
                     /gene="fabK"
                     /locus_tag="VW157_000488"
     CDS             complement(25310..26284)
                     /gene="fabK"
                     /locus_tag="VW157_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9551839.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(26403..26627)
                     /locus_tag="VW157_000489"
     CDS             complement(26403..26627)
                     /locus_tag="VW157_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9551840.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(26687..27661)
                     /locus_tag="VW157_000490"
     CDS             complement(26687..27661)
                     /locus_tag="VW157_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9551841.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(27661..28095)
                     /locus_tag="VW157_000491"
     CDS             complement(27661..28095)
                     /locus_tag="VW157_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9551842.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(28189..28974)
                     /locus_tag="VW157_000492"
     CDS             complement(28189..28974)
                     /locus_tag="VW157_000492"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9551843.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            29299..30663
                     /locus_tag="VW157_000493"
     CDS             29299..30663
                     /locus_tag="VW157_000493"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9551844.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRELTPIKEEEILRQLVQKTKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGK
                     SKED"
     gene            30666..31037
                     /locus_tag="VW157_000494"
     CDS             30666..31037
                     /locus_tag="VW157_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9551845.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            31250..32524
                     /gene="serS"
                     /locus_tag="VW157_000495"
     CDS             31250..32524
                     /gene="serS"
                     /locus_tag="VW157_000495"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9551846.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(32548..33447)
                     /locus_tag="VW157_000496"
     CDS             complement(32548..33447)
                     /locus_tag="VW157_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9551847.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLTE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(33557..34105)
                     /locus_tag="VW157_000497"
     CDS             complement(33557..34105)
                     /locus_tag="VW157_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9551848.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(34323..35645)
                     /locus_tag="VW157_000498"
     CDS             complement(34323..35645)
                     /locus_tag="VW157_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9551849.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFTALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSQVVIAETKKYFDK"
     regulatory      complement(35736..35824)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(35988..38324)
                     /locus_tag="VW157_000499"
     CDS             complement(35988..38324)
                     /locus_tag="VW157_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9551850.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQEGQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     SDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRVLKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKRV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(38428..38976)
                     /locus_tag="VW157_000500"
     CDS             complement(38428..38976)
                     /locus_tag="VW157_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9551851.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(38973..39275)
                     /gene="zapA"
                     /locus_tag="VW157_000501"
     CDS             complement(38973..39275)
                     /gene="zapA"
                     /locus_tag="VW157_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9551852.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            39362..40243
                     /locus_tag="VW157_000502"
     CDS             39362..40243
                     /locus_tag="VW157_000502"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9551853.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            40247..40861
                     /gene="lepB"
                     /locus_tag="VW157_000503"
     CDS             40247..40861
                     /gene="lepB"
                     /locus_tag="VW157_000503"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9551854.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            40989..43355
                     /locus_tag="VW157_000504"
     CDS             40989..43355
                     /locus_tag="VW157_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9551855.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSNGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMILAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGKQKSQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(43403..44686)
                     /locus_tag="VW157_000505"
     CDS             complement(43403..44686)
                     /locus_tag="VW157_000505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9551856.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVITAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYSKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        13024 a        10232 c         8506 g        12955 t
ORIGIN      
        1 ctctacacca taaatatttg ctaacaaaaa tactgtatta ttaccctctt aaggtgcgaa
       61 attattcaag ttcttaaaaa atgcttctct atttccgtaa gatagcttat ttcttcgatt
      121 taagaactga agatgatagt tctaaccttt agaaatccaa gccttcacta gctcaaaaac
      181 tcctatcttt tttcctcaat aaatcatctg agagataggt attccttata tccttcaaat
      241 tcatcactcc tttggatatt gaggtgctag cctagaagca gacacccgat tatctaaacc
      301 atccttaata gagatgacaa gacagataag ctcagttaat tacttatata tctttttaat
      361 atggtatatt tgtcaacaaa aagaaatcaa atcatacctt atactcaatg aaaatcaaag
      421 agcaaactag gaagctagcc gcaagttgct caaaacactg ttttgaggtt gcagatagag
      481 ctaacgtggt ttgaagagat tttcgaagag tattaaagac agtgtgacta tgcttgtcaa
      541 tagataggaa atcaaaatga aggatggaaa tctgagccaa acatcataag tgctataatg
      601 aacagaagta agaattgcac gataagtttt ctaaaaccat aggaatcgtc ccctttcttc
      661 atcttcatta tagcatatat acatcagttg tacaaataat atgatatttt tttattagtc
      721 ctcagacaag catattataa agcgtttcat taccatttaa gttaatataa gctggatcaa
      781 aaccttccat tctacgaatc aaacctgcat aatcatgctt atctgctaaa gcgatcccaa
      841 ttaatactgg gcctgttccc ttgctagctc gtttgatata ctcaaaacgt gtgatatcat
      901 catttggccc caggatatca tttacaaact cacgcaaagc tcctggacgt tgtgggaaat
      961 tgaccacaaa ataatgtttg ataccatcat aaatcaaggc acgctcttcc atttctggca
     1021 tacggttgat atcattattt cctccagaaa tgatacaaca aatggttttc cccttaatat
     1081 attcagctaa aacctctaaa gaggcgatac tagctgctcc agcaggttct gcgactatcc
     1141 cttgcttaga gtaaaggtca atcaaggttt cagaaatcaa tccctcatcg acacctacca
     1201 aagtttgaat atgttgacga gttgcttcat aggtcaactg acctaccttt tgcacagcaa
     1261 tcccatccgc aaatttatca atttccttga gtttgactgg acctccagcc tcaaaggcag
     1321 ctttcatgga acgcgctcca ttcgcctcta ctccgataac ctcaatctct ggacttgttt
     1381 ccttgatata ggtagaaacc ccggcaatga gaccgccacc accaacagga accaagacag
     1441 catcaaaatc aatcgattct tttcgagctt cttctaaaat ctcataagca actgttcctt
     1501 gacctgcttg aacatgagca tcatcaaaag gatcaataaa ggtacgattt tcagagactg
     1561 taaattcttg agctgctttg gctgaggcat caaaggtatc tccaactagt ttaatagtta
     1621 caaaatcccc accaaaaaag cgaacctgac caatcttttg ttgtggcgta gtaatgggca
     1681 taaagatagt agcaggaatt ttcatttcat tacaagtata ggctactccc tgcgcatgat
     1741 ttcccgcaga agcgcagact accccacgtt cacgttcttc cttgctgagc tgggaaatgg
     1801 cataataggc accacgaatt ttaaaggagc gaacacgctg ggcattttct tttttcaaat
     1861 aaatcttagc accatacttc tccgataaat aatgatcgta atccagtgga gtattcacaa
     1921 ccacaccgtt caagaccttg tgagccttga ttatatcttt tgaacttaac atataaaacc
     1981 tctttttcta taaaaaacag tcactaatga ctgtttttta gttattcatc tccgaaaact
     2041 tcagtaacac tttcatggta tttatgagcc atacgaatcg aaaactcttt aggaagttca
     2101 taacgatttg tggaaatcca atcatataac cattctacca aactttcttc actatcatta
     2161 cgaaagtttt cgcagtccag tcatttaaac ccgattcttt caatccagat gcaataattt
     2221 gctctatttg atataaaccg ccatctatcg tctcatttat tctgcgaaga ggaccttcaa
     2281 tataggataa agcttctccg aatctaacag ctgatttagg tcctaatgat ttaccaacta
     2341 tatttgttaa tgttttttat ttctcaaaat ccatttcgct atataacgag cagatttagt
     2401 cgcaccagct ctctcaaacg gagaatgaat ttcaagacct tcttctctag atatttgtgc
     2461 caatttgaag aattgttcaa agttcatatg caacctacca tcttcaaaat tagcatcatc
     2521 ataaatgtta attacagcat tttcatcata cgaccaatct gctttatttt ggtctgctag
     2581 cactattacc gacggtgtaa ttacattata caactagaac caataataac gatgcaattt
     2641 ttctcataat ctttttcata aaatcatctc cagcgctata tatttctaat tagttataga
     2701 ttttgaatgc atcatcgtcg tttttaccaa cgaatggcat tgctttacgc aattctgcac
     2761 caactttttc aatttcaagg ttagctgctt gttcacggta agcagtcaat tttggacgtc
     2821 cagctttata gtcatttaca aagtcatttg caaatttacc attttggatg tctgccaaga
     2881 cagccttcat attttcttta acttgttcag tgattacacg tggacctgat acatagtcac
     2941 cgtattcagc agtgtttgaa atagattgac gcattttctt gaatccacct tcgtagatca
     3001 agtcaacgat caatttcatt tcgtgaagaa cttcaaagta agccaattct ggagcgtaac
     3061 ctgcttctgt caagacttcg aaacctgctt cgataagggc agtcaaacca ccacaaagta
     3121 cagcttgttc accaaacaaa tcttcttcag tttcttcttt gtaagttgtt tcaagaagac
     3181 ctacacgagc cgctccaaca cctttacacc agtccatagc aatgttttta gcatttcctg
     3241 ttgcatcttg gtatactgca taaagagctg gaacaccaaa tccttcttcg taagtacgac
     3301 gtaccaagtg tcctggtcct ttaggagcac acatgaagac atctacatcc gcaggaactt
     3361 tgataaattc aaagtggatg ttgaaaccat gggcaaatcc aactgcgttt ccagcttcca
     3421 agtttggagc gatttctgct tcgtacaatt cttgttgaat ttcgtctggc gccaagatca
     3481 tgataacatc agccaactta gtagcttctg ctactatgta agtatcaaat ccatcttctt
     3541 ttgctttatc aaaagattta cctggacgta caccgataat aacgtcacga cctgaatcac
     3601 gcaagttttg agcatgcgca tgcccttgtg aaccataacc gataacggcg atttttttac
     3661 cgtcaagtgc tgctacttta acatcttttt catattccat ttgaactgtc atagcttttc
     3721 tctcttttct attatttatt gccttttaag ctggtttaac aaatttaagt tggattttta
     3781 atcgcgggta aatccagttg cacccgttcg agcaatattg cgaataccgt atgggcgaat
     3841 gactcgcaat agggcttcgc tcttttctgc atttcccgtc atctgaatgg taatcgagct
     3901 tggcgctacg tctaccactg ttgcacggaa aggttgaata atcgctaaaa tctcagctct
     3961 ctcctcagct ggcgctgaca tcttaaccaa aatcacctcg cgctccaaat gaggcttgtc
     4021 tgtaatatct cgaatgcgaa tcacatcaat ctgacgattg agctgtttga tgatttgctc
     4081 cacttcatca tgagaagcaa catcaataat aatagtgata cgcgatacat tcggatcttc
     4141 tgttactcca acagagatgc tttcaatatt aacctgacga cgagatagga cacctgtaaa
     4201 gcgattgagg actcctgaac gattttgtag ttttgctgtt aacattctac gcatggaact
     4261 tcacccccaa catctcatga ttactcttac cagccggtac cattggtaac acctgttcct
     4321 tacgagaaat atctacctca attagcatag gaacatcctc agtgatgact tcaaggtctt
     4381 gagccaaggt ctcaggatta tcaaacttat agtttttaat accataagcc tgcgccatca
     4441 attggaaatc aggaagggta tcaaagaccg actctgatgt tctgccttca tagaaggatt
     4501 cctgccactg gcgaaccatt ccaagtgaat gattgttcag cataaccacc ttgattggca
     4561 ccttgtaaat attcaaaata gccaactcct ggttggtcat ttggaaacca ccatccccaa
     4621 caaacaagac tacttcctta tctgggttag caattttagc accgattgct gctggaattc
     4681 caaagcccat tgttcccaaa ccacctgaag tcactaactg acgctcattt tggtagggat
     4741 aatactgagc tgtccacatt tggtgttgac caacgtctgt taccacaatg gcatctccat
     4801 tcgtcaattc accaattcgt tcaataactg cttgcggttg aaccacacgc tctttcttgt
     4861 cataagaacg aacacgattc ttgtctttag tgactttctc aatccacttt tcagtattgt
     4921 tgtgaactgt tggttctgct agcaacattt gcaaggcctt cttagcatct ccaactacag
     4981 gaatgtctgc actgataatc ttgccaatct cagctgggtc aatatcaatg tgggcaacct
     5041 tagcattctt agcgaaagtc ttaggattcc ccgtcaaacg gtcatcgaaa cgagaaccaa
     5101 tactaatcat aaagtccgct tccgtcatgg caatatttgc tgcgaatgac ccgtgcatgc
     5161 ctcccattcc aagaaagagt gggtgactcg ttgcaatcgt tccttgtccc aaaagactgg
     5221 ttaccactgg aatttgatag cgttctgcaa attcatttag ttccgtagca gcctcagcat
     5281 aactaattcc accaccagct aacaagactg gctttttagc cttggacaat tgcttcaaga
     5341 ttttcttgat ttgcatatca ttcggctcaa gagtcggctg ataacttggt aaattcactt
     5401 ctggtgaata aatgaagtct gtttctaaag cagatatgtc ttttggtagg tcaattacaa
     5461 ctggccctgg acggcctgta gttgcgatat ggacagcttc cgtaatgata cgcggaatat
     5521 cagctgtctc acgaacttgg taattgtact tagtgattgg catggtaatt cctacgatgt
     5581 ctgcctcctg aaaggcatcc ttcccaatcc ctgctcgcgc cacctgacct gtaaagacca
     5641 aaaggggaac gctatcgctc atagcatccg caatccctgt aatggcattt gttgctcctg
     5701 gtccactagt gacgacggca acacccaact ttccagttga tttggcataa ccttcagctt
     5761 catgcaaaca accttgctca tggcgcccta gaatgtggcg aatgccttta aaattatata
     5821 tcgcatcata aaaaggcaaa accgcaccac caggataacc aaagatggta tcaactccta
     5881 aatcacgaag tgtttccaaa actaggtccg accccgtctt aggagattct aaactgattt
     5941 tctccattgt tcccctttct ttcctcttaa aaataacttg ttactatcat accatttttt
     6001 caaaattttt caagataaaa gaagaaattt tctgaatttt ctattttaac gtttatttat
     6061 gaatgttatt tttgttttta ttaaaaagat accgatttca tttactaaaa aagaaaaaag
     6121 aactgatttc tcagtccttc attaatctta ttccacacta aatagatatg ggtaaacagg
     6181 ttgttgacct tggtgaatct cgacttcaac gtcttcgaat tcttctacga tttcttgagc
     6241 gatttcattg gcaagttctt cgcttccgtc ttcaccgaca tagaaggtta cgatttcact
     6301 atcttcatcc aacatatgtt tcaaggtttc agtcaatgtt tggtgcatat cagggtttga
     6361 cacaagaatt tttccatcca ccatacctag attatcgttt tcatggattt ctaagccatc
     6421 gatcgttgta tcacgcacgg ctgttgtgac gcttccgcta acgacatcgc taagagcagc
     6481 tgtcatacgc tcttggtttt cttcaatgga cttgcttgga tcaaaggcaa gaagacttgt
     6541 cataccttga ggaagagtgc gagcctctac cactactgct ggttgctcca aaacttctgc
     6601 cgcagattga gctgccatga agatgttctt gttgtttggc aagaagatga tgttacgggc
     6661 attgacctgt tcaacagcct tgataaagtc ttctgttgaa gggttcatgg tttgaccgcc
     6721 ttcgataaca taatccacgc cttgagaaca gaagatatct gctagacctt taccagccac
     6781 cacagcaatc aaagcatact ctttttcttc agccgacttg ataacttgag tagcttcttt
     6841 ctcaacctgt gcttcgtgtt ggttacgcat attgtcaact tttaccttga ccaagctacc
     6901 atatttgaga ccttcttgca taacaagtcc tggatcttct gtatgaacat ggactttgac
     6961 aatttcatca tcgttaacaa cgaggagaga atccccaagc tcatccaagt agttacggaa
     7021 ttcatcgtag tcaaaatctt tagcataggt tggaccttgc ttaagagcta ccatgatttc
     7081 agtacagtaa ccaaacgtga tgtcctcagt tgctacgtga ccagctacag acttatgatg
     7141 ctctacattg atcatctcac tcatgttggc aggagtcgct acaaagtcct cagatgcaat
     7201 atattcgcca gtaagggctg aaaggaaacc ttcgtagatg aagaccaatc cttgaccacc
     7261 tgagtccaca acgccaactt ctttcaatac tggaagcatg tctggtgttt tagctagagc
     7321 tgttttagca ccttccaagg ctgcgcgcat gacttcaaca gtgtcatctg tttgctcagc
     7381 ttttttctta gcaccgatag cagctccacg agaaactgtc aaaatcgttc cttcaacagg
     7441 tttcatcact gccttatagg caacttccac acctgattgg aaggccagag ccaagtcttg
     7501 acctgttaac tcgtctttat ccttgatagc ttgggaaaat ccacggaaaa gctgagacgt
     7561 aatcactcct gagttcccac gcgcacccat caaaagccct ttggcaagaa tgctcgctac
     7621 ttctccaact gtagaagctg gcttgtctgc aacttcttta gcaccatttt caatggtcat
     7681 tcccatattt gtcccagtat ctccatctgg aactggaaag acgtttaatg aattgacata
     7741 ttcagcttgc ttattcaagc gagttgatgc agcctgcacc atttcttgaa ataagctagt
     7801 agtaattttt gacacggtta ttctcctaca actttgatat tttgaatgta gacatttaca
     7861 gtctgagcag taattccaag ctggttttcc aagctaaagg caacacgctc ttgaatgttt
     7921 tttgacactt cactaatctt tgttccgtag cttaacacgg tatatacatc aactgcaata
     7981 ctgccatctt cggctgcctt tacgacgaca cctttagaat aattttcctt acctagcagg
     8041 gcttggaaat tatctttgag ggcattttta ctagccatac cgaccacacc aaaaatctca
     8101 gttgctgctc cacctacgac ggttgcaatc acttcatctg ttagttcgat ttgaccatct
     8161 tttgtattaa tttttacagt catccttttt acctcaacta gttgatactc tattttatca
     8221 tatttcagcc caagtgtaaa agcagaatga tgtatcagcg gatatttact ctatttttca
     8281 aacgatgtta tacccacaat aaaagaaaaa agaccctaag gtctcctttg cttttattat
     8341 taaacgcgtt caactttacc tgatttcaaa gcacgagctg aagcccaaac ttttttaggt
     8401 ttaccatcga taagaacagt aactttttga aggtttggtt ttacggcacg ttttgtttgg
     8461 ttcatcgcgt gtgaacggtt gtttcctgat acagtcttac gacctgtaaa gtaacatact
     8521 ttagccattg tgttttcctc ctattagatc taatatagcg gatgtgctag caccacatac
     8581 cgtactatgt tatcacactt tcttgttttt tgcaagggaa ttggaagatt ttttatttgt
     8641 gtcttaaatc aggtcttgcg tgacattttc tgctctccac atgccattgt tgattgacag
     8701 aacaccagaa ttaaaattat gtgtataaaa atcatctcta actgcagcta agggtatagc
     8761 cgtcaagtcc aaatcccaca gctcatctat cgattttctt acaacaatat ctaaatccaa
     8821 atacagtaca cgagactcgc ttacatactt tggaataaaa tatctaaaaa agccgcatat
     8881 gaaagtccct caaaggggag acgataacct ttcagaatac tactgtcaat ctaaacattc
     8941 acaatctcac tattcaaagt ctctagtctt ttttccatca attggaacca ttctcgcgga
     9001 aggtcatcat taaaaacata aaacttaaga ttataataga tgaacacaaa gagattttat
     9061 tgttgtttca actttatcca tataagcatt atctgcacct aagacaatcg cttttttctc
     9121 ttctttcact ttttatctca tttcttttta ttcccatcat attattccca tcatatgttt
     9181 ctacgtaacc attattttcg cctattcgtt cgtaaaacca taccagtgga gattttagat
     9241 gaagtcccat tacggtttac aatttttaca ttacgacacg gagttttaca aatcgatttc
     9301 atttgccaaa cgtagttagt aaggcagtta gctagttcgc caaatagcga ctagcgtcca
     9361 acaatttgga actttagttc caattgttgg tactgagtca catcttctcc tctaactcta
     9421 cgtctggata cttgtccgca aaccagcgga gggcaaagtc attttcaaag agaaagactg
     9481 gttggtcaaa acggtctttg gctaagatat tgcgacttga cgacatccgt tcatccaagt
     9541 cctcaggctt gatccaacga acggtctttt tacccattgg gctcatgacc acttccgcat
     9601 tgtattcgcc ttccatacgg tgtttaaaga cttcaaactg gagttgacca acagctccca
     9661 gcatgtactc acctgtttgg taattcttat aaagctgaac ggctccttct tgcaccaatt
     9721 gctcaatccc cttgtggaag gatttttgct tcataacatt cttagcagaa actttcatga
     9781 aaatttcagg agtaaaggtt ggcagtggtt caaattcaaa cttgtttttt ccaaccgtca
     9841 aggtatcccc aacctgataa gtaccggtat cgtaaacccc gataatatca cctgccacgg
     9901 cattgatcac attctcacga ctttccgcca taaactgggt aacattagat agtttagccc
     9961 ccttaccagt acgagggaga ttgacactca tgccgcgctc aaattcgcct gatacgatac
    10021 ggacaaaggc aatacggtca cggtgacgag gatccatgtt ggcttggatt ttaaagacaa
    10081 agcctgagaa atccttgtca taaggatcca caatttcacc gtctgttttc ttgtgaccat
    10141 gtggttctgg agcaaacttg aggaagattt caaggaaggt ctgcacacca aagtttgtca
    10201 gggctgaacc gaaaaagaca ggcgtcaatt ctccagccag aatagcttcc tctgaaaact
    10261 cattcccagc ttcatttaaa agctcaatgt catccttgac ttgctcgtag aaaggattgc
    10321 taccaaaaag tttgtctcca tcttctaggc tagcaaaacg ctcatcccct ttgtaaagct
    10381 ctaaacgttg gttatagagg tcatacaagc cctcaaaggc tttccccatc ccgataggcc
    10441 agttcatagg gtagctagca atgcccaaga tttcttccaa ttcttgcaag agatccaaag
    10501 gctcacgacc gtcacgatcc aacttgttca taaaggtaaa gactggaatg ccacgatgtt
    10561 tcacaacctc aaacaatttc tttgtttgag cctcgatccc cttggcagag tccacgacca
    10621 tgaccgcagc atccaccgcc atcaaggtac gataggtatc ttctgagaag tcctcgtgcc
    10681 ctggcgtgtc taagatattc acgcgcttgc cgtcgtagtc aaattgcata acagatgaag
    10741 taacagaaat cccacgttgc ttctcgatat ccatccagtc agatttagca aaagtccctg
    10801 ttttcttccc ttttaccgta ccagcctcac gaatctcacc cccaaagtag agtaactgct
    10861 cagtgatggt tgttttcccc gcgtccgggt gggagataat ggcaaaggta cgacgtttct
    10921 taatttcttc ttgaatattc ataagttctc tttctttgat tctctatttt tcttgtttca
    10981 atagctgaga atgattttta cattggattt taccattcct ttcgacactc cattatatcg
    11041 gattttagca tttttttcaa tttctatttc ttttcacttc cccctccctt atttatagga
    11101 aaatatggta aaatagaaca gactaaaaat catcatttca cgaaaggatg caagatgaaa
    11161 attacgcaag aagaggtaac acacgttgcc aatctttcaa aattaagatt ctctgaaaaa
    11221 gaaactgctg cctttgcgac caccttgtct aagattgttg acatggttga attgctgggc
    11281 gaagttgaca caactggtgt cgcacctact acgactatgg ctgaccgcaa gactgtactc
    11341 cgccctgatg tggccgaaga aggaacagac cgtgatcgct tgtttaaaaa cgtacctgaa
    11401 caagacaact actatatcaa ggtgccagct atcctagacg atggaggaga tgcctaatga
    11461 cttttaacaa taaaactatt gaagagttgc acaatctcct tgtctctaag gaaatttctg
    11521 caacagaatt gacccaagca acacttgaaa atatcaagtc tcgtgaggaa gccctcaatt
    11581 catttgtcac catcgctgag gagcaagctc ttgttcaagc taaagccatt gatgaagctg
    11641 gaattgatgc tgacaatgtc ctttcaggaa ttccacttgc tgttaaggat aacatctcta
    11701 cagacggtat tctcacaact gctgcctcaa aaatgctcta caactatgag ccaatctttg
    11761 atgcgacagc tgttgccaat gcaaaaacca aaggcatgat tgtcgttgga aagaccaaca
    11821 tggacgaatt tgctatgggt ggttcaggtg aaacttcaca ctacggagca actaaaaacg
    11881 cttgggacca cagcaaggtt cctggtgggt catcaagtgg ttctgccgca gctgtagcct
    11941 caggacaagt tcgcttgtca cttggttctg atactggtgg ttccatccgc caacctgctg
    12001 ccttcaacgg aatcgttggt ctcaaaccaa cctacggaac agtttcacgt ttcggtctca
    12061 ttgcctttgg tagctcatta gaccagattg gaccttttgc tcctactgtt aaggaaaatg
    12121 ccctcttgct caacgctatt gccagcgaag atgctaaaga ctctacttct gctcctgtcc
    12181 gcatcgccga ctttacttca aaaatcggcc aagacatcaa gggtatgaaa atcgctttgc
    12241 ctaaggaata cctcggtgaa ggaattaacc cagaggttaa ggaaaccatt ctaaatgccg
    12301 ctaaacactt tgaaaaattg ggtgctattg tcgaagaagt cagccttcct cactctaaat
    12361 acggagttgc cgtatactac atcatcgctt catcagaagc ttcatcaaac ttgcaacgct
    12421 tcgacggtat ccgttacggc tatcgcgcag aagatgcaac caaccttgat gaaatctatg
    12481 taaacagccg aagccaaggt tttggtgaag aagtgaagcg ccgtatcatg ctgggtactt
    12541 tcagtctttc atcaggttac tacgatgcct actataagaa ggctggacag gtccgtacac
    12601 ttatcattca agatttcgaa aaagtcttcg cggattacga tttgattttg ggtccaactg
    12661 ctccaagtgt tgcctatgac ttggattctc tcaaccatga cccagttgcc atgtacttag
    12721 ccgacctatt gaccatacct gtaaacttgg caggactgcc tggaatttcg attcctgctg
    12781 gattctctca aggtctacct gtcggactcc aattgattgg tcccaagtac tctgaggaaa
    12841 ccatttacca agctgctgct gcttttgaag caacaacaga ctaccacaaa caacaacccg
    12901 tgatttttgg aggtgacaac taatgaactt tgaaacagtc atcggacttg aagtccacgt
    12961 agagctcaac accaattcaa aaatcttctc acctacttct gcccactttg gaaatgacca
    13021 aaatgccaac actaacgtga ttgactggtc tttcccagga gttctaccag ttctcaataa
    13081 aggggttgtt gatgccggta tcaaggctgc tcttgccctc aacatggaca tccacaaaaa
    13141 gatgcacttt gaccgcaaga actacttcta tcctgataac cccaaagcct accaaatttc
    13201 tcaatttgat gaaccaatcg gatataatgg ctggattgaa gtcaaactag aagacggtac
    13261 gaccaagaaa atcggtatcg aacgtgccca cctagaggaa gacgctggta aaaacatcca
    13321 tggtacagat ggctactctt atgttgacct caaccgccaa ggggttccct tgattgagat
    13381 tgtatctgag gcagatatgc gttcgccaga ggaggcctat gcttatctga cagccctcaa
    13441 ggaagttatc cagtacgctg gcatttctga cgttaagatg gaagaaggtt ctatgcgtgt
    13501 ggatgccaac atctcccttc gcccttatgg tcaagaaaaa ttcggtacca agactgaatt
    13561 gaagaacctc aactccttct caaacgttcg taaaggtctt gaatacgaag tccaacgcca
    13621 ggctgaaatt cttcgctcag gtggtcaaat ccgccaagaa acacgccgtt acgatgaagc
    13681 taacaaaaca accatcctca tgcgtgtcaa ggaaggggct gctgactacc gctacttccc
    13741 agaaccagac ctacccctct ttgaaatttc tgacgagtgg attgaggaaa tgcggactga
    13801 gttgccagag tttccaaaag aacgtcgtgc gcgttatgta tctgaccttg gtttatcaga
    13861 ctacgatgct agtcagttga ctgctaataa agtcacttct gacttctttg aaaaagctgt
    13921 tgccctaggt ggtgatgcca aacaagtctc taactggctc caaggggaag tcgctcagtt
    13981 cttgaatgct gaaggtaaaa cactggaaca aatcgaattg acaccagaaa acttggttga
    14041 aatgattgcc atcatcgaag acggtactat ttcatctaag attgccaaga aagtctttgt
    14101 ccatctagct aaaaatggcg gtggcgcgcg tgaatacgtg gaaaaagcag gtatggttca
    14161 aatttcagat ccagctatct tgatcccaat catccaccaa gtctttgccg ataacgaagc
    14221 tgctgttgcc gacttcaagt caggcaaacg taacgccgac aaggccttta caggattcct
    14281 tatgaaggca accaaaggcc aagccaaccc acaagttgcc cttaaactac ttgcacagga
    14341 attggcgaag ttgaaagaaa actagacaga acaaaaccag ccctaaggtt ggttttttct
    14401 tgacaaacag aaagaaaacg tttacaatat tacgtgtaaa gtttccaaac aagaaaacta
    14461 aaaatcgcaa caataaggag ttatctatat ggctacaatg aaggcagctc gctggcatgc
    14521 agcgaaagat gttcgtattc aagaaataga agttcctgaa gtacttccgc accaagtaaa
    14581 agttgcagtt aagttcacag gaatttgtgg tactgacctc catgaatttt tagatggacc
    14641 tatctttatc ccaacagatg agcacgtcta ttctggtcaa aaagcaccgg taacactggg
    14701 acatgaattt tctggtgaaa tcatagaagt cggaagcgat gttacccgtg ttaaagttgg
    14761 tgatcgtgtt gctgtggaac caattctagc taaaaataac ttagttggtg attataattt
    14821 ggatccaaac cttaattttg tcggtttggc tgcagacggt ggatttgcca aatattgtgt
    14881 tttagacggt gacttagtac atgtcattcc agatagcttg agctatgagc aagctgctct
    14941 tactgaaccc gctgctgttg cagtctatgc tgttcgtcaa tcttctttga aggctggcga
    15001 tacagctgtt gtctttggtt taggcccgat tggtctatta attgtagaag ctcttcgtgc
    15061 agctggtgct tctaagattt atgctgttga actatcacct gaacgtcaag caaaagctga
    15121 agagttagga gctatcgttg tccgtccgga agaaggtgaa acagctgttg aagctattca
    15181 tcgtttaaca aacggtggtg tagatgtttc ttatgaagta acgggtgtgc cagtagtttt
    15241 aggacaagcc ttagctgccg tacataaagc aggtgagtgc atggtggtct ctatctggga
    15301 acgagaagca aatattaatc cgaatgaatt tgcaatccaa gaaaagacac taaaaggaat
    15361 cattgcttat cgtcatatct tccctaaagt attagaattg atggaacaag gctacttctc
    15421 tgctgaaaaa ttagttacta agaaaattaa attggaaaat atcgtcgaag aaggctttat
    15481 cgaattaact caagataagt cacaaattaa aattttggta gaaccagaat aaaaaatcaa
    15541 tctttttaag acaatactaa caggtcggag taaacttact tcggcctttt gaaatatagt
    15601 actcttcgaa aatctcttca aaccacgtca gcgtcacatt accgtatata tggttactga
    15661 cttcgtcagt tctatccaca acctcaaaac agtgttttga gcagcttgcg gctagcttcc
    15721 tagtttgctc tttgatttcc attgagtatg agaagaaata tagtggatac tctttaacat
    15781 taaccactcc acctgactag ccaatattct ttttcgctcc taccaactcc caataactat
    15841 tttggcttta tttccagagt attttatggt aaaatgaaga gtaataatat ttgttaaaga
    15901 ggtaaaaaca tgattgaagc aagtaaatta aaagctggta tgacctttga aacagctgac
    15961 ggcaaattga ttcgcgtttt ggaagctagt caccacaaac caggtaaagg aaacacgatc
    16021 atgcgtatga aattgcgtga tgtccgtact ggttctacat ttgacacaag ctaccgtcca
    16081 gaggaaaaat ttgaacaagc tattatcgag actgtcccag ctcaatactt gtacaaaatg
    16141 gatgacacag catacttcat gaatacagaa acttacgacc agtacgaaat ccctgtagtc
    16201 aatgttgaaa acgaattgct ttacatcctt gaaaactctg atgtgaaaat ccaattctac
    16261 ggaactgaag tgatcggtgt caccgttcct actactgttg agttgacagt tgctgaaact
    16321 caaccatcta tcaaaggtgc tactgttaca ggttctggta aaccagcaac gatggaaact
    16381 ggacttgtcg taaacgttcc agacttcatc gaagcaggac aaaaactcgt tatcaacact
    16441 gcagaaggaa cttacgtttc tcgtgcctaa tctctagaaa gaggtcattc tatgggaatt
    16501 gaagaacaac ttggcgaaat cgttatcgcc ccacgtgtac ttgaaaaaat cattgctatc
    16561 gctactgcaa aggtagaggg tgttcactct ttttcaaaca gatcagtgtc tgataccctt
    16621 tcaaaacttt cactcggccg tggcatttat cttaaaaacg tggacgaaga actcacagca
    16681 gatatctatc tctaccttga gtacggagta aaagttccta aggtagcggt tgctatccag
    16741 aaagctgtca aagatgccgt ccgtaatatg gctgatgtag aactcgctgc tatcaatatt
    16801 cacgttgcag gtatcgtccc agataaaaca ccaaaaccag aattgaaaga tctatttgac
    16861 gaggacttcc tcaatgacta gtccactatt agaatctaga cgccaactcc gtaaatgcgc
    16921 ttttcaagct ctcatgagcc ttgagttcgg tacggatgtc gaaactgctt gtcgtttcgc
    16981 ctatactcat gatcgtgaag atacggatgt acaacttcca gcctttttga tagacctcgt
    17041 ttctggtgtt caagctaaaa aggaagaact agataagcaa atcactcagc atttaaaagc
    17101 aggttggacc attgaacgct taacgctcgt ggagagaaac ctccttcgct tgggagtctt
    17161 tgaaatcact tcatttgaca ctcctcagct ggttgctgtt aatgaagcta tcgagcttgc
    17221 aaaggacttc tccgatcaaa aatctgcccg ttttatcaat ggactgctca gccagtttgt
    17281 aacagaagaa caataaaaag aaaaagtgtt tgatagatat caaacacttt tttctttgcc
    17341 tcccactatc taaaaaaatg ataatagata taattgtaaa caaaaatcca gataggtttt
    17401 gcatgattga gaaagttaaa aaaactatgg cagagaatcg ttaatctcag attgtcggta
    17461 gaacgataaa caagggcaaa aaagaaacca atcagactat aatataataa actaattgga
    17521 tctctgtgag atagtatcaa atggctaatc ccaaagatga tagcagatag gataacatcc
    17581 aaatagtact tggactaggg aaagaaggta ttcataaaat accctctatc aagagtctcc
    17641 tcaaaaacag gaccgatgat tacaggcagg acaaaagata agatagtcga taaaaaggtt
    17701 ggttgtccat ttgaaaaaag cacggtaaaa tactcatcat gaatattcct atgattaatc
    17761 aaatgagcat agcgtgccca aaaattaccg agaatctgat aaaccacata agttgcaaat
    17821 aagtagaaga caaatgacca gttccagctc tttttctcaa agataaagag catctttttc
    17881 ttttttaacc tccaaattaa tagaaggaaa cttcccacta atcccattgt taaaataaga
    17941 gaatagacat cagctcctaa ccctaaaatg atcgtcacat acaatccaat tgtttgtggt
    18001 agatagtaaa ataataagca aaaatattcc aaattgtctt agtttttttg tgtttctcat
    18061 cgtacttttt tgaaagatta ccctgctcgg aagccgtact tccaagcatc tatataagaa
    18121 ttaagtgccc cttgcctcat atagggagca aattctctat aatataacca tctactatat
    18181 ccatcttccc aaacagcaag accacctgaa gtttgctcca agtcctcagt tgaaagaact
    18241 gtaaatgtat ttgtacctgt cattgcaagt accttcttaa aatagattgt tgtaggctca
    18301 catttatagt atatttcttt ttttgtctat tttatagccc atctcctcaa ctggcaattt
    18361 ttcgacctga attacatttt tccataaaaa atgagacctt tctagtctca tttagtcatt
    18421 cttagtattt tctaaatcgt tgatagcgtt cttccagcaa ctcttctagc ggtttttgtg
    18481 aaagtctagc cagctctgtt tggagttctt ttttgacact cttaatcagt tctttactag
    18541 aaagtcctat ttcagaaatc accttatcta ccacgtccat ttctaacagt tcatgcgaag
    18601 tgattttcat cagttctgct gcttccatag cgcgagtacc gtccttccat aaaatggaag
    18661 caaagccttc tggactgaga atggcataga tagaattttc cagcatccag acacggtccg
    18721 cgacagctag agccagagcc ccgcctgaac caccttcacc gataataatg gcgataatag
    18781 gaactttcag gtcactcatt tccatgagat tgcgagcgat agcttcccct tgaccacgtt
    18841 cttccgctcc gacaccagga taagcacctg ctgtattgat aaaggtcaca actggacggc
    18901 caaatttctc agcctgtttc atcaaccgca gtgcctttcg gtagccttct ggatgtggtt
    18961 ggccaaaatt ccgtttgagg ttgtcttgca aactcttgcc tttttggata ccaaccactg
    19021 ttacagcttg atctccaagc caaccaatac caccaacaac tgcaccatca tcacgaaaag
    19081 aacggtcacc atgtaattgg ataaattcat caaaaatgcc tgtcgcaaag tccaaggttg
    19141 tcaagcgact ctgctcacgc gcttctctga ctatttttgc aatattcatc taggactccc
    19201 tccatgcaat ctgactaggc tagcaatcgt atctggtaag tctcttcttt tgacaatagc
    19261 atccacaaag ccatgttcta ataggaattc tgccttttgg aaatcctcag gcaagctttc
    19321 acgaaccgta ttttcaatca cacgacgccc agcaaaacca accaagctct gtggttcagc
    19381 cagaatgata tcgccttcca tagcgaaaga agctgtcaca ccaccagtcg ttggatctgt
    19441 caaaatggtc aggtaaaaga gaccaacatt tgaatggcgt ttaaccgccg cagagatctt
    19501 agccatctgc atgagactca tgattccttc ctgcatacgg gctccaccag aggctgtgaa
    19561 taggacaact ggcaattttt cgacagtcgc atactcaaac aaacgagtga ttttttcacc
    19621 tacaaccgta cccatagaag ccatgataaa gttagaatcc ataatcccaa gagccacagt
    19681 ctgaccttta ataagagcag ttcctgtcac aacggcttca tgcagacctg ttttttcacg
    19741 catagatgcc agtttctttt ggtaaccagg gaaatgcaag ggatccttgc tttcaatccc
    19801 tgtaaacaat tctttgaagg ttcccatatc aatcgtcaaa gccaagcgtt cttgggcaga
    19861 aatacgaaag gtatagctac agtgcggaca gatacgttca cttcccagat ccttctgata
    19921 gatggtatgc ttacagcctg gacactggga aaataattca tctggaacct ctggcttagc
    19981 ttgaggtttt tccctaaccg aacgattggg attgattcga atatacttat cttttttact
    20041 aaatagagcc attgattccc cttttcggtt taaactctta aagtcatttt attctttttc
    20101 ttgatattta ggtaagaagg tttccatcaa gaaggaagta tcataatccc cagcaatgac
    20161 attgcgatct gaaatgaggt caagctggaa atctgcattg gtctgcactc cttcaatctc
    20221 taattcatag agggcacgtt gcattttcat caaggcgtca aaacgatttt cgccgtgtac
    20281 tatgattttg gcaatcatac tatcataata aggcggaatg gtataacctg gataaactgc
    20341 tgaatccacg cgcaagccaa ctccaccact tggcagatag agattagtaa tcttacctgg
    20401 acttggagca aagttaaagg ctgggttttc tgcattgata cgacactcga tggcatgacc
    20461 gcgtaggaca atatcttctt gcttaacaga caaaggctga cctgccgcaa tgcaaatctg
    20521 ttccttaacg atatcaacac ctgaaacaaa ctctgttact ggatgttcta cctgaacacg
    20581 agtattcatc tccatgaaat agaaattgct acttgcttca tcaagaagaa attcaatggt
    20641 tcctgcattc tcatagccaa caaactctgc cgctcgaaca gcagcagcac ctatttcatg
    20701 acgcagcgtt tttccgattg caatcgaggg actttcttcc aaaacctttt ggttattcct
    20761 ttgaagagaa caatcccgtt cacccaagtg aatcacatgt ccatgctcat cacctaggat
    20821 ttgaacctca atgtgccgag ctggatagat aacccgttct atgtacatgg caccattgcc
    20881 ataattggcc ttggcctcac tagaggcagt ttcaaaggca gaaacgaggt catctggttt
    20941 ttcaacctta cgaatccctt taccacctcc acctgctgaa gccttgagca taacaggata
    21001 gccaattttt tcagcaacaa tcaaagcttc ttcagagtta tgcacttctc catctgaacc
    21061 tggtataaca ggcacacctg ctttaatcat ctgagcacgc gcattgatct tatcccccat
    21121 catatccata acatgaccag atggaccgat aaacttgata cctacttctt cacacatggt
    21181 cgcaaatttg gaattttcac tgagaaatcc aaaaccaggg tgaatagctt ctgcctcagt
    21241 caagactgca gctgatagaa ctgcattaat attgagataa gactctgttg ccttgccagg
    21301 accaatacaa actgcttcat ctgccaaaag cgtatgaaga gcttccttat cagcagttga
    21361 ataaaccgct accgtcgcaa tccccaattc acgtgccgca cggataatac gaaccgcaat
    21421 ttcaccacga ttggcaatta aaatttttcg aaacatggag aacctcctta gttcccaatt
    21481 gcaaaagtaa ggataccact ggctgcaagc ttgccatcca cttcagcctt tgcttcaacc
    21541 acagctatgg tgccacgacg ttttacaaaa gtcgctgtca taaccaattg gtcgcctggt
    21601 acaacttgct tcttgaactt aaccttgtcc ataccagcgt aaaagaccag ttttccttta
    21661 ttttcaggtt ttgataactc caacacacca gcagtttgcg ccaaggcttc cataatcaga
    21721 acacctggca taactgggta ttgaggaaag tggccgttaa agaaaggttc gttaatggtc
    21781 acatttttga tagcaacaat ggtatcctcg ctcacttcca agacacggtc cactagaagc
    21841 ataggataac ggtggggaag agcttctttg attccttgaa tatcgatcat ttgatacgta
    21901 ccaatccttt accaaactca accatttctt cgttagagac gagaatttcc gttaccacac
    21961 catccttagg agctgggatt tcattcatga ctttcatggc ttcgataatt accaatgttt
    22021 gacctttttt gacactatca ccaactgtaa cgaaggcagg tttatctggt ccagcagcca
    22081 agtaaaccac tccaacaagt ggactctcta caagatttcc ctcactagcc acacttgctt
    22141 cagctggagc tggaacttct tctgctacag tctctgctgg agcagatgta ggagctactg
    22201 gactcggtgt tgctagaacg ggtgctggag cgacttgagt tgcaacttca ggcacaggtc
    22261 tcgcttcatt cttgctaaac tgcaactcat ccgtcccatt tttataagaa aattctctca
    22321 aacttgactg gtcaaattga gtcatcaagt ctttaatatc gtttaaattc atacttatct
    22381 attctcccaa cgtttgaaag caagaactgc attgtggcct ccaaaaccaa aagtatttga
    22441 aatagcgtat ggaatttctt tctccaagcc ttgtccataa acgacattag cttcgatata
    22501 atctgatact tcacttgtcc cagctgtcat tggtacaaag ttatgacgca tagcttcgat
    22561 ggtgacgata gcttctactg cacccgcagc ccccagcaaa tgtcctgtaa aagacttggt
    22621 tgatgataca ggtacttcct taccaagaac agctacgata gcaccacttt ctcctttttc
    22681 attggcagga gttgacgttc cgtgagcatt gacataggct acttgctctg gagaaatctc
    22741 agcttcttcc aaggctagtt tgatggcctt gatagctccc tgaccttctg gatgtggaga
    22801 agtcatgtgg taggcatcac aagtatttcc gtaaccaacc acttcagcca ggatagtagc
    22861 tccacgtttt tcagcgtgtt caagactttc tagaaccaac atccctgaac cttcacccat
    22921 aacaaaccca ttgcgatcct tatcaaatgg gatcgaagca cgagttggat cctctgtagt
    22981 agagagagct gttaaggctt ggaaaccagc gatggcaaaa ggtgtgatag aagcttctgt
    23041 tcctcccacc aacatcacat cttggaaacc aaacttaatg gagcggaagg catccccaat
    23101 cgcatcattt gatgaagagc aggcagtatt gatagattta caaacaccgt ttgcaccaaa
    23161 acgcatggct acattcccag aagccatatt tggtaaagct tttggaagag tcattggttt
    23221 gacacgtttg ggtccttttt catgaaggcg aagtacctga tcttcaattt ccttgattcc
    23281 accaatacca gatgcaacga taacaccaaa acgatcccta ttaagagcct ctacatcaag
    23341 attggcatga tttacagcct cttgggctgc atacaaggca tataaagaat agttatcaaa
    23401 acggttggta tcttttttta caaagtattt atcgaacgga aaatcttgga tttctgccgc
    23461 attatgcaca tcaaagtcac tatgatcaaa ttttgtaatg ccaccaatgc cgattttccc
    23521 agttgctaaa ctattccaaa attcttctgg tgtatttccg attggagatg ttactccata
    23581 acctgttacc actactcgat ttagtttcat tcttttcacc tctagctttc gctacatact
    23641 taagccacca tcaatggcaa ccacttgacc agttagataa tcttggcctg ctaaaaatac
    23701 tgtcaaatct gcaacctgct ctgcctgccc aaattctttc atcggaatct gagctagtgt
    23761 agcttcctta atcttatctg ataagatagc tgtcatatca gactcaatca ttcctggagc
    23821 aatcacattg actcgtatat tccgactagc gacctcgcgt gccacagact tggtaaagcc
    23881 aatcaagcca gccttagaag cagcatagtt agcttgacca atattcccca tcaaaccaac
    23941 aacactagac atattaatga tagcaccttc tctggctttc atcatcggtt tcaagactga
    24001 ttgtgtcata ttaaaggcac cagtcagatt gaccttgagc actttttcaa aatctgcttc
    24061 tgtcatcttg agcataagag tatcttgggt aatccctgca ttgttgacca aaacatctac
    24121 tgaacccagt tctgcaatag cttgatcaat catacgctta gcgtctgcaa aatctgatac
    24181 atctcctgaa atgggaacca ccttgatacc atagtttgaa aactcagcga gcaattcttc
    24241 tgagattgcc ccacgactgt ttaagacaat gttggctcct gcttgagcaa acttgtgggc
    24301 gatggcaaga ccaattccac gactcgaacc tgtaataaag atatttttat gttctagttt
    24361 catttttttc ctttcaaaac ttctacttat tttagtctat ttttctaaaa gtgctactaa
    24421 actcgcttga tcttccacat gagctaagtg agcagtttga tcaatttttt taacaaaacc
    24481 tgacaagact ttccccggtc caatctcgat aaagttgctt atgcctgctt cttgcatgac
    24541 cccaatactt tcatagaaac gaacgggttc cttgacctga cgcgtcaaga gctgagcaat
    24601 gtcctctttt tgcatcacag cagcttctgt attgccgact aggggacaag taaaatctga
    24661 aaaacttacc tgagctagag tttcagctag tttctggcta gcaggctcaa ggagagcggt
    24721 gtgaaaggga cctgacacct taagaggaat caagcgtttg gcacctgctt cttgcaaaag
    24781 ttcaaccgct cgatcaactg caaccacttc tccagcaatg acgatttgtg caggtgtgtt
    24841 atagttggct ggagtaacca ctccaagttc agaagctttt tgacaggctt cttcaatgac
    24901 ctctactggc gtattgagaa ctgctaccat cttgccagag tcagcaggag ccgcttcttc
    24961 catataggct ccacgcttag ctaccaaggc aaccgcatct tcaaaatcca aggcgccgct
    25021 tgccaccaag gcagagtatt ctccaagaga caaaccagca accatatcag gctgatagcc
    25081 cttttcttgc aataaacggt agatagcaac cgaagtcgct agaatggctg gttgcgtata
    25141 gcgggtctga ttgagtttgt cttcttccgt atcgatgaga taacgtaaat cataaccgag
    25201 cacctgactc gctcgatcaa tcgtttcttt gacaatcgga tactgatcat agaaatcccg
    25261 tcccatccct agatactggg caccttgacc agcaaataaa aaggctgttt tagtcatttc
    25321 ttacaactcc tgcccagcga gaggcttctt cttgaatttt cttagcggct ccgtaataca
    25381 aatcttttag gatttcttca gctgtttctt ctttagaaac aagccctgcg atttgacctg
    25441 ccatgacaga gccaccatcc acatcaccgt gaacaactgc tttggctagg gcacctgctc
    25501 ccatttgttc aaagatttct aaatcaggat cttcctgctt aaaggcatct ttttcagcca
    25561 gttcaaaatc tctagtcaac tgatttttaa tagcacgaac agcatgacca aagtgctgag
    25621 ctgaaatcgt agtatcaata tcccttgctt ttaaaatttt ctccttgtag tttggatggg
    25681 cattcgactc ttttgcaact acaaaccgtg tccccacctg tacagcctct gcacctagca
    25741 taaagccagc cgcagcacct tcaccatccg caattcctcc tgcagcaata acaggaatag
    25801 atatagctgt ggctacctgt cgcaccaagg tcatggttgt taatttaccg atatgccccc
    25861 cagcttccat tccttctgca ataacagcgt ctgcaccgat tttttccatg cgtttagcta
    25921 aagcgacact aggaacaaca ggaataacga ttatcccagc ttcatggaaa cgttccatat
    25981 acttgcttgg atttcctgct cctgttgtga caactttaac accttcttca ataacgagat
    26041 ccacgatatc ttccacaaag ggagataaaa gcatgatgtt gaccccaaag ggtttatcag
    26101 tcaatgattt gattttatca atattggcct tgacaacttc tttcggggca tttcccccac
    26161 cgataattcc taatcctcca gccttggaaa cagcccctgc caaatcacca tcagcaaccc
    26221 aggccatccc tccttggaaa ataggatagt caatcttcaa taattctgta atacgcgttt
    26281 tcatagtgcc tccaaccttc cttgcttacg taatagttcg atttcaccat aatttgacag
    26341 tcaaactatt acctaaacaa gagggagtgg gtttctccct actccttcta ctaatattct
    26401 gcttattttg cttgctcttc aacgtaagca accaagtcac caactgtttt caagtcattt
    26461 tctgcttcga tttggatatc aaaagcatct tcgatttctg agattacttg gaacaagtcc
    26521 aatgaatctg cgtccaaatc atcaaaagtt gattcaagtg ttacttctga tgcgtctttt
    26581 ccaagttctt caacgataat ttcttgtact ttttcaaata ctgccatgat aggactcctt
    26641 taaaataaat agttttttta taacaatgtg ttcaccacat gattacctaa attgtaagaa
    26701 tgagcgtgcc ccaggtcaag cctccaccga agcctgatag aagaacagtc tggctaccat
    26761 ctaaagggat gagaccttgt tctacacact ctgaaagtaa aatcgggata ctggctgcac
    26821 tggtattgcc atattccatc atattggctg gaagtttggc tcggtcaaca ccaatttttc
    26881 tagccatctt atccaaaata cggtcattgg cttgatgaag tagcagataa tccaagtctg
    26941 tcacctctat aggagattca tcaatagtct gcttgataga cttggctaca tctcgaatgg
    27001 caaaatcaaa gactgtgcgt ccatccatct tcaaaaacga atctgcactt tcttgatctg
    27061 aaaatggaga atgcaaacct gaatgcccat aagttaaaca ctcgctgcga cttccatcgc
    27121 tattgagact ctcagctaag aaatgctctt gctcgctagc ttctaacaag acaccaccag
    27181 caccatctcc aaacaacaca gctgttgatc gatccgacca atcgactgcc ttagagaggg
    27241 tttcactacc aatcaccaag cctttttgaa agcgaccaga agcgataaac ttttcagcag
    27301 ttgaaagagc aaatacaaat ccactgcaag ccgcggttaa gtcaaaagca aaggctttat
    27361 tagcgccaat attagcttga acacgagcag ctgtagaggg catcatcgaa tctggagtaa
    27421 tggtagctag gatgataaaa tccagttctt ttcctgttat tccagctttt gccatcagtt
    27481 tcttagcaac ctctgtagcc aaatcactgg tagattctgt tcttgaaata tgcctttgtc
    27541 gtattcccgt tcgacttgaa atccactcat cattggtatc cataatctga gccaagtcgt
    27601 gatttgtaac cacttgctct ggcacataat gagcaacctg acttattttt gcaaaagcca
    27661 ttatttcaaa tcctccaaaa attggtaaag attagtcaag cctttaccca tgacagcaat
    27721 ttcttcctcg ctcatgccat caataatttt ttctaccatg gccttgtgga agcgtttatg
    27781 cagtctatga atcaagcgac ccttctttgt caaatgcaga tgcaccacac gacgatcctg
    27841 ttctgaccga actcgctcaa tgtagccctt acgctctaaa ttgttcaaac ttgtcgtaac
    27901 agttccaaga gttaccatca actcttttga cacttgactt ggagtcacat ctggagcctt
    27961 accgatgaca tcaatcgtat gcatttcttt gatggagata tccttaaaac gactacctct
    28021 caagttcact tcctcaatta caaggacatt gttaaatata gatgttaaat attcattgat
    28081 tcgttggtag tccatttttc atatccctcc ttcttcaaaa aacttttaca atcaaaacat
    28141 ttgatagaaa gaattatatc aaaattcaaa gaatgatgca agtatttttt attttcctgt
    28201 aaatttaggt cttcttctct ccgaatgagc tcgcactcct tctttgaaat cctctgtttg
    28261 agctaaggat ttctgtaggt tcagttctaa agtagcgtaa ccctgccagt ctttaaactg
    28321 gctctcccaa accaacttct taatggcagc ataggaatta cttgaagcac gtcttaattt
    28381 tttaagaagc tgttctctcg tcttttcaag tttttcagct tcagaaacgc ggtaaaccaa
    28441 cccccattct agggcttttt ctgccgttaa agcctctcct gtcatagcta attgagcagc
    28501 acgcgtcaca ccgatactgc ggctcaagag atgaattccc cctgcatctg gagccaaacc
    28561 aacaccaaca aaggcttgga taaacttagc cttatccgtc gccaaacaga aatctgccgc
    28621 aacagccata ttcgctgcgg cacctgcaac agcaccgtca acttccatta aaacaggttt
    28681 ggctatttgc ttgattttat aagaaatcgt attgaccaac tctgcgattt ttgtcaatga
    28741 tggaatatca tcctcatcca ctgcccgctt catctctacc aaatctcccc caactgagaa
    28801 gacctttcca ttggcattga ttaagataaa atgcacagct ggattttctt ctgccaaagt
    28861 cagagcttct aaaatctcct cacacatggg aatatgaaaa ccatttgcga cctcaggacg
    28921 gttcaaggta aggattgcca agtcctcttc aagctgataa ataatgtgtt ccatcagggc
    28981 tcctttttat tttataagca gtttatactc ttcgaaaatc tcttcaaacc gcgtcagctt
    29041 ccatctgtaa cctcaaaaca gtattttgag cagtctgcgg ctagcttcct agtttgctct
    29101 ttgattttca ttgagtgtta aattatttca ctgtcaaagt atatcttttt taggagcaga
    29161 aagcaagaaa aaatcgactg aaagtgtctc agccgataag aaaggctcgc tttaggctag
    29221 aaagcatctt attttcttca ttgaaaaaag cctttctttc tgctataatc gtataaaata
    29281 cttactttag gagttcttat gaaagttgtt aaatttggag gtagttctct tgcctctgct
    29341 agtcaattag aaaaagtttt aaacatcgtc aaaagcgatt cagagcgtcg ttttgtagtc
    29401 gtttctgcgc ctggtaaacg caatgctgaa gatactaagg ttacggatgc cctgattaaa
    29461 tactaccgcg actatgttgc gggtaacgat attagcaaga gccaaagctg gattatcgac
    29521 cgctatgctg ctatggttag tgaattggga ctaaaaccag ctgtgctaga aaaaatttct
    29581 aaaagcattc acgccttggc cactcttcct attgaagaaa atgaatttct ctacgatact
    29641 ttcctagcag ccggtgaaaa taacaatgcc aaattgattg ctgcctactt taaccaaaat
    29701 ggtatcgatg cacgctatat gcaccctaga gaagctggga ttgtggtcac aagtgaacct
    29761 ggtcacgctc gcatcattcc atcaagttat gacaagattg aagaattgac aaacaccaat
    29821 gaagtccttg tcattcctgg tttctttggt gtcactaagg aaaatcaaat ctgtactttc
    29881 tcacgtggag gttctgatat tacaggttct atcattgctg ctggtgtcaa agctgacctc
    29941 tatgaaaact ttacggacgt tgatggtatc tttgcagccc accctggtat tatccaccaa
    30001 ccacactcga ttcctgagtt gacctaccgt gaaatgcgcg agttggccta tgcaggcttc
    30061 tcagtccttc atgacgaggc tcttcttcct gcctaccgtg gaaaaattcc tctggttatc
    30121 aagaatacca acaaccctga ccatccaggt actcgtatcg ttctaaaaca cagtaatgat
    30181 gaatttccag ttgtgggaat tgctggtgac tcaggctttg tcagcattaa catgtcgaaa
    30241 tacctcatga accgtgaggt tggatttggc cgcaaggttc tgcaaatcct ggaagaactt
    30301 aacatcggtt gggaacatat gccaacaggt atcgacgatc tttctatcat tctccgttct
    30361 cgcgaactaa ctcccatcaa ggaagaagaa atcctgcgtc agttggttca aaagactaaa
    30421 gtagatcatg cagaaatcga acacgacctt tctatcatta tgattgttgg tgaaaaaatg
    30481 aagagccaca tcggagtgac tgctactgcg acacgcgctc tatctgaaaa caaaatcaac
    30541 atccagatga tgtctcaagg ttctagtgaa gtttctatta tgtttgttgt caataaagac
    30601 caagagaaag cagcaattaa agccctctac aatgcctttt ttggaaaaag taaggaagac
    30661 taagcatggc ccaatcactt aacaaaacag tactcctcag tacgacaggc acttcctacc
    30721 tctctatcgc tgggaaagtt gggaaattcc ttgtcggaga tcaggctttg gaattttacc
    30781 cagatgtcaa tgtcgaacaa tttatccaga ttccttggag ccatatcaat cagattggag
    30841 ccaatgtcac tggacgcaaa atcagccgcc acttcgaagt ctttacagac aaaggaaaat
    30901 tcctctttgc ttcaaaagac tcaggtgcca ttctcaaaat tgcacgcgaa aaactaggca
    30961 atgacaaggt cgtcaaactt ccgaccctga ttcagacaat tagccaaaaa tttaaaaatc
    31021 tatttgcaaa aaagtagaaa ctttagtata atcatagcaa cggataagta ggttatcttc
    31081 ttctttcaga aagtctgcgg gtactgcgag cagataggag ggataggtga aattctaccg
    31141 ttagtgacaa ttacatacac aatcaagtgc acacctgtga agttggatgg aaccgtggcc
    31201 ctgccactcc aacgttttgt cagggtgctt ttttcataaa ggagttctta tgttagatat
    31261 caaacgtatt cgtacagatt ttgaagctgt cgcagaaaaa ttagctacac gtggtgtaga
    31321 tgctgctgtc ttgaatgaaa tgaaagaaat cgatgctaaa cgtcgtaaca tcttggtcaa
    31381 ggttgaaact ctcaaagcag aacgtaacac agtttctgct gagattgccc aagctaagcg
    31441 caacaaggaa aatacagatg acaagattgc tgccatgcaa aatctatctg ctgaggttaa
    31501 agccttggat gctgaattgg cagaaatcga tgctaaattg acagaattta caacgactct
    31561 tccaaatatc ccagctgaca gcgttcctgt tggggctgac gaagacgaca atgtggaagt
    31621 tcgccgttgg ggtactccac gcgagtttga cttcgaacct aaagctcact gggatctcgg
    31681 tgaagacctt ggtatccttg actgggaacg cggtggtaag gtaacaggcg ctcgcttcct
    31741 cttctataaa ggcctcggtg ctcgtttgga acgtgctatc tacaacttta tgttggatga
    31801 acatggaaaa gaaggctata ctgaagtcat cacaccttac atagtcaacc atgattctat
    31861 gtttggtact ggtcagtatc caaaatttaa ggaagatact tttgaactca gcgataccaa
    31921 ctttgtcttg attccaactg ctgaagttcc tctgacaaac tactaccgtg atgaaatctt
    31981 agacggcaaa gatcttccaa tctacttcac tgctatgagt ccgtcattcc gttctgaggc
    32041 tggttctgcc ggtcgtgata cgcgtggctt gatccgtttg caccaattcc acaaggttga
    32101 aatggtcaaa tttgccaaac ctgaagaatc ttacgaagaa ttggaaaaaa tgacagccaa
    32161 cgctgaaaac attcttcaaa aactcaacct tccataccgt gtcgttgctc tctctactgg
    32221 agatatgggc ttctcagcta cgaagactta cgacttggaa gtgtggattc cagcacaaaa
    32281 caattaccgt gaaatctcaa gctgttcaaa cacagaagat ttccaagccc gtcgtgccca
    32341 aatccgttac cgtgatgaag cagatggcaa ggtgaaactc cttcatacct tgaacggttc
    32401 tggacttgca gttggacgta cagtggctgc aattcttgaa aattaccaaa atgaagatgg
    32461 ttctgtgacc atcccagaag cacttcgtcc atacatgggt ggagctgaag tcatcaaacc
    32521 ataaaaaaaa ggtttagcta tttctagcta gacctttttt cgtaaccaaa tcagataagc
    32581 acctagtaca aagaataaaa tagttaggca tataatggtt tcagtcaata ccaggtaatc
    32641 cagaaatgga agtttcaaaa ttccctgagc catcttgagc gaggtcgctg tgataatggt
    32701 tgggaaggtg agggctgaga aggctggttg aaaaccttgt tttaaaatgt tgggcagacg
    32761 agttaaaaca aagaaaaaga aggattgaga agccaaaatc atgacaatca agacccaagt
    32821 cggcaggctg gttcctccta ctcgaactag agaagccaag agtagagaga aaggagcaca
    32881 gtagattcct tcttgtccaa gcaaggctag tgggagtgga tgtttcttta aatcgctata
    32941 aataagggga tagagataga aggtcaagag aaaaccaaaa ctcaaggtcg cataggcaat
    33001 ttcgataata cctaccagag gataggtcaa ggcagccact gctatcccca catagagaac
    33061 cgtccaactt ggagtggcat gaaccctccg ccctggacaa gcaaacttga tggtaaaacc
    33121 agcaatcaag gtcaaatcca agagaaatga aaaccaccaa atcccttgtg ctaccaaagg
    33181 aagataagag aatacgcgaa agacataggt cgataaaatc atcccagcca taggaaaggt
    33241 tgccattcct gacaaaagag ggggcttggt caattcttgc ttggtttctt tccaattaaa
    33301 gagatgcaga attagaaagt aaatccataa aaccaaacca gtcagactaa aaagatggga
    33361 tagaaccggc aacgtatcta aaataagatt tccagctcct gccaaaccta gcaaacaacc
    33421 tgaaaatact aaggggagtt ttttcatcct aacctccaat aatcatgtta gtttcagtat
    33481 aacataaaag cgcttaaatg aggatttaaa aaaacgaagt ccgcttattt cagacttcat
    33541 tttactcaga tatgaattag gcataaggtt gcaattctgg attaattggt gtattagcta
    33601 agttgttggc atagttacag aggattgcta ggctgacacc aaaaaccaca tccaaggcat
    33661 tttgttgagt atagccagct tctaaaaact cagacaaggc ttcatctcct acacgaccct
    33721 tggtattgat aactgccaag gtaaacttag ctagggtatc caatttagga tctgtttcaa
    33781 ttggagtacg attgcgaaga gcttgaatca agtcatcatt catctggatt tgtttgatgg
    33841 aaaaggctgt gtgacctgcg acacagaagg cacaaccatt ggtcacggct gccgtgattt
    33901 gcaccacttc acgctcaacg ggtgtcaggc tgttgcgacg gtggatagat gagacaattt
    33961 ggtaggcttc taaaacagtc ggggcattgg ccaagagacc gattaggttg ggaatatagc
    34021 cattgttgtc tttttctact gtttcaagaa tttctttcac ttctgctggt gctgactcta
    34081 ctgtatggat agtaaatgtt gtcataagat acctcttttc ttattattga cactaatatt
    34141 attggaaaat cttataaaat cctgattcct aagtttatct aagataaagc tttattctct
    34201 cataagattt tcgttgttat attagtttat cacacttcca atcacttgca taatatatat
    34261 tatatatcag gctgataaaa attatttata ggcaaaaaaa tcacacgagc tgtgtgattc
    34321 cattatttgt caaaatactt tttagtttca gcaataacga cttgcgacaa gaccaagagg
    34381 gcaatcaagt ttggcagagc catcaaggcg ttaacgatat ctgcgataat ccagaccata
    34441 tccaactcga taaatcctcc taacaagacc atgagcacaa aaaccacacg gtagagccag
    34501 ataaagcgaa ccccaaagag gaactcaaaa cagcgttctc cgtaatagtt ccaacctaga
    34561 atcgttgtaa aggcaaaaag tacaaggaag atggtcaaga gggcaggccc aaagtgtgaa
    34621 aagactgttg agaaagctga ctgagtcaag gcaaccccat tcaagtcacc actccaaact
    34681 ccagttacca agatggtcaa accagttaga gtacaaatga tgagggtatc aataaaggtt
    34741 cctgtcatgg aaatcaaacc ttgctctact ggttcatttg tcttggcagc tgcagctgca
    34801 ataggagcag aacccagacc agattcgttt gaaaacacac cacgcgccac accattttga
    34861 atagccatcc gaacgctagc accagcaaat ccacctaccg cagcaagggg actaaaagct
    34921 gaggtaaaga ctaaagcgat tgtgccaggg atttttccga tattaaagaa aataactgta
    34981 agagttccta agatataaat gatggccata aaaggaacaa cagtagttga aaccttagaa
    35041 atagacttga gcccaccaaa gactgcaatc gctacaaaga cagacaagac gagagctgtg
    35101 atggctggcg aaatcgtcgt tgtattttgg atagattctg taatcgagtt gacttgggtg
    35161 aaggttccga ttcccaagag agcaaccaat actcctgcta ctgcaaacaa aacagcaagt
    35221 ggtcgccact tttctcccat ccctagaagg atataatgca tgggacctcc cgctactgca
    35281 ccatggtcgt ccttggtgcg gtatttgatg gccaagagtc cttccgcata cttggtagcc
    35341 attccaaaga aagccgccat ccacatccaa aatagagctc ctggtccacc aaccttgata
    35401 gccgtcgcaa ctcctatgat atttcctgtt ccaacagttg atgccaaggc tgtacacaga
    35461 gctgtaaaac tggatacatc accatgtccc ttatcctgga taaaaataag ctgaaaggcc
    35521 ttgggcagac gcaaaacctg caagagtcct agccgcatgg ttaggtaaat ccctgttccg
    35581 accaataaaa tcaagagggg cggtccccaa gcaaaagcat cgattgattt aagcaattct
    35641 aacatttcct tctcctatcg tttcaacccc aaaagaaaga gcacatgcaa gatacatgta
    35701 ctctggaatg cttagataaa tgctaaaaag cggtctatcc tagctctgtc cttttacctg
    35761 agagtttgag cagttgcctg ccttgcccct tcggtgcctt tatggtctct ccagagttcc
    35821 gtccatttac agtcatggaa aatcaaacga ttccccactt ctattaaact tcattcggtg
    35881 ttggtattta attgattcta attttacaaa aaatgttggc ttttgtcaat gtgtttatta
    35941 gtaaaaatta gttcaacagt ttttacttta taaagtccag aatactgcta tcctttaaaa
    36001 gtgacaatag tcgcaccact gcctccagca ttttgtgggg catagccgaa actcttgaca
    36061 tgtttgtttc tttgcaagta tttggtaact ccttcacgga tgactcctgt tccgatacca
    36121 tggatgatat caacttgagc catattgtta agcaaggctt ggtcgatgaa ggtatctagc
    36181 tcattcatgg cttcttcata gcgcttgcct cgaagatcca gtctagcttg aggtcctcgc
    36241 ccagaagttc gtttcacaac attgacctgt ttcttcttga ctcgtttttc ttgctgggct
    36301 tgaacaagat caaactcttt ctcttccaag gtcatcttaa tcaagccaac ttgggcttcc
    36361 cagcggccgt ccttgagttg actggtcaag gtaccacgct gaccataact gagaaccacg
    36421 atatcatctc ccacctttgg agctcgtttt ttcttggcct tttgaaggac cttgttttta
    36481 gacaagtcca ctttttcagg agccaatttt ttcaacttgg ctttggcttc aatgatttcg
    36541 tggggcttga gttgggattt actgtggaga tttttgagaa tctggtcact ttcacttagg
    36601 gccatatcca caatctcagc agcctgttca cgcgccttgt taagctcggt ttccttttca
    36661 cgattaagct cgttgtagag tttttttagc acacggttca tcttgagatt ttcttgctcc
    36721 acctcacgga tattgtccaa gcgtttgcgg ctttccagcg tctgctcttc taattgctca
    36781 atgatacgat tgacgtcact gtcctgatcg atctgctgac tggcatctcc tacgataact
    36841 tcagataggc ctagacgttt ggcaatttca aaggcattac ttcggccagg aacaccctgc
    36901 ataaagcgat aggtcgggcg aagagttgca gtatcaaact ccatactggc attttgcaca
    36961 aaggctgtct caataccgta ggccttgagt tctggatagt gggtcgtcgc catggtcttg
    37021 atttgacgca ggcgaaggtc ctccagaata gccatggcaa gggctgctcc ctcttgggga
    37081 tcagtaccag cccccaactc atccaaaagt aagagtgaat gttggttgac cttgccaaga
    37141 atatccacga tattggtcat atggctagag aaggtagaca agctctgctc aatagactgc
    37201 tcatctccaa tatcagcaaa gatttcttca aaaataccaa cacgacttcc cttgtctgct
    37261 aaaatcggca atcctgactg ggccatgacc tgtgtcaagc ccagagtttt gagcatgatg
    37321 gtcttcccac ctgtattggg acctgtaatg acaatagctg ttaaatcttg accaaaatag
    37381 acatcatttg cgacggcatt tttgaccaaa ggatggcaga catggagcag ttgaatctct
    37441 tgattttctg acagctgagg cacgactgct tgtccttctt ggataaatcg aaccttggca
    37501 cgaatcaagt ccagatgacc gataatccaa gcgtcattag caatctcagc cgcatgaggg
    37561 cggacacgct cagaaatttc ttggagaatg cgaagcattt catagcgctc atctgctcgc
    37621 agactagcaa tttcttcgct cagtttgact acctcacgtg gttcgatata gacggtgttt
    37681 ccactagcag aaatatcatg aacgacacct gcaatcttat tgcggtaggt gtttttgact
    37741 ggtaaaacct gacggccatt tctgctagca acaattcctt ccgtcaacat ctgcgctttt
    37801 tgcttgagca agtcttgtaa aacatcgcgt acctgactct cgctatcatg tatttttcga
    37861 cggattcgcg ccaattcttc actggcaaaa ttttcaatga aacccgcatc attaaaggcc
    37921 tgaagatttc cttgtaattg cggaaaatca tgtaatttct caaaccaaag ggctaattct
    37981 tccaagctga cattttccag attggcgtaa aaactttgaa gttctcggct ggcaagaagc
    38041 acgcgtttca agagtaggaa ctcctcgata ttgagatccg ctcccatctc caacctcttg
    38101 cagactcctg caatttcctt agttgagaga atagtaaaat gcggttgctc gacgaaaaga
    38161 gcctgcattt ctttcatctc agcaaaagcc tgtttgattt tatctgcttt ggcagtcgga
    38221 gccagctgtc tcaattgctc caagccctgc tcggtcaaca aatgaggctc aaacaaggcc
    38281 ttgaccttat cgaactctaa tgtttctaat attttcttat tcatctttat ttccttgtct
    38341 ttgaatgtct aggtgtggta gctttttaca ttctgataga ttcgagtcaa atctgtaaac
    38401 aaagggctag gaaaactcct gcccttttta tccgattaaa tttgtcaccc agatttgttt
    38461 gagccaactg gttgttatcg gtatgctctg gatgatgtgt tttgcgacga tactcttttc
    38521 aagaggattt tgtataactg ccatggggat ggtcgccaag attgtcaagg ccatttgcaa
    38581 gacaaataag gtcaccaaca tagacaagat acctgctgaa acttggaaca acttaccacc
    38641 cagttttttg ctaggaatca agtgtaagag aagaccaagc aaacgaccaa tgctatagac
    38701 aatcccaaat acaagcaagt agccgatacc tgcataaaag accttatcca gctgaaagag
    38761 ttgatccgat gggaaaaaga aagtcccctg accttcctgc gaatttgcat aagggagcaa
    38821 taaatggaat tgctctccaa gccccttata aaactggcca gccataaaag ccgatgccat
    38881 ggctgaaatc aggtaataaa cctgtaagag caggcctctc cgatagccga tataaaatcc
    38941 ccaaaccaag accaatagaa gaaggaatga aatcataagg aatcctcaat cttgctctgt
    39001 tcttgcttac aagtcacaag cttgtgacgg agttcttcta gctcttgctc cttatcgtca
    39061 aattcaatct cacggctgag ctgagttgat aaacagttga ctgccaacaa aagagcgatt
    39121 gtttcatcat ctgcgctagg catttgttct ttaattgctt ggtatttttc tgtcgcaacc
    39181 ttagcgattt cctccataaa aaggttatca tgttcgcttg tcaaggttaa cgattttttc
    39241 ccgaatgtaa atttgaatcg atttagattt gccataaaat tcacctcacg atattatacc
    39301 aaaattcgct aattttgtca gtttttacaa attttactgc ttttgtggta caatagaaac
    39361 tatggcaagt atcacactca caccaagcga aaaggatatt caggcttttc ttgaacacta
    39421 tcaaaccagt ctggctccta gcaagaatcc ctatatccgc tactttttga aactacctca
    39481 agcaacggtt tctatctata cttctggaaa aatcttgctt cagggtgaag gggctgaaaa
    39541 atacgccagt ttctttggct atcaagctgt agagcaaacc agcggacaaa atcttccttt
    39601 aattgggaca gatgaggtgg gaaatggttc ctactttggt gggcttgcag ttgtggctgc
    39661 ctttgtcaca cctgaccagc acgacttttt acgaaaactc ggtgtggggg attctaagac
    39721 tctgaccgac caaaagatcc gtcagattgc tcctattctc aaggaaaaaa tccagcacca
    39781 ggcactcctt ctctcaccca gcaagtacaa cgaggtcatc ggagaccgct acaatgctgt
    39841 ttcggttaag gttgccctcc ataatcaggc tatctatctc ctccttcaaa aaggtgttca
    39901 gcctgagaaa attgtgattg atgcctttac cagtgctaaa aattatgaca agtacttggc
    39961 acaagaggcc aatcgtttca gtaatcctat cagcttagaa gaaaaggctg agggcaaata
    40021 cttgtctgtc gcagtttctt ctgtcattgc gcgtgatctc tttctggaaa atcttgaaaa
    40081 tctgggacga gaactgggtt atcagcttcc aagtggagct ggaacggctt ctgacaaggt
    40141 ggctagccag attttgcaag cctatggtat gcagggactc aacttctgcg ccaaattgca
    40201 ctttaaaaac actgaaaaag cgaaaaaacg cttagaaagg taagttatga attcatttaa
    40261 aaatttctta aaagagtggg gactgttcct cctaattctg tcattactag ctttaagtcg
    40321 tatctttttt tggagcaatg ttcgcgtaga aggacattcc atggatccga ccctagcgga
    40381 tggcgaaatt ctcttcgttg taaaacacct tcctattgac cgttttgata tcgtggtggc
    40441 ccatgaggaa gatggcaata aggacatcgt caagcgcgtg attggaatgc ctggcgacac
    40501 cattcgttac gaaaatgata aactctacat caatgacaaa gaaacggacg agccttatct
    40561 agcagactat atcaaacgct tcaaggatga caaactccaa agcacttact caggcaaggg
    40621 ctttgaagga aataaaggaa ctttctttag aagtatcgct caaaaagccc aagccttcac
    40681 agttgatgtc aactacaaca ccaactttag ctttactgtt ccagaaggag aataccttct
    40741 cctcggagat gaccgcttgg tttcgagcga cagccgccac gtaggtacct tcaaagcaaa
    40801 agatatcaca ggggaagcta aattccgctt ctggccaatc acccgtatcg gaacatttta
    40861 agaaacctaa gaggccgaga atcaccaatc tcagcctctt cttctatcgt gagaaaatga
    40921 ttggtaacta tctaaactta ccagaacaga aacacctcaa ctctcaccta ttcatgcaaa
    40981 ggaattttat ggaagtttat ttttcaggaa ctattgaacg gattattttt gaaaatccca
    41041 gcaattttta tcgcatcctc ctcctagaaa tcgacgatac ggacgcagag gattttgatg
    41101 attttgaaat cattgtcaca ggaaccatgg ctgatgtaat tgagggcgaa gactatactt
    41161 tttgggggca aattgtccag cactccaagt atggagaaca actgcaaatc agtcgttatg
    41221 atcgcgcaaa accaactagt aatggcttgg tcaagtactt ttcaagtagc catttcaagg
    41281 ggattggtct caagacagct cagaaaatcg tggataccta tggcgaaaat accattgacg
    41341 aaattttgca acacccagaa aagttagaag gcatcgcagg actctctgcc aaaaatcgcg
    41401 aggctttcgt ctccactctc cgtctcaact acggaacgga gatgattttg gccaaactag
    41461 ccaactacgg cattcccaac aaactagcct ttcagattca agacttttac aaggaagaaa
    41521 cccttgatgt ggttgaaaat tatccctacc agctggttga ggatatcaag ggtttgggct
    41581 ttaccattgc tgaccaacta gctgaggaac taggcatcga aagtcaggct cctgaacgct
    41641 tccgcgccgg tctagttcac agtctttttc aggcctgtat ggaaacaggg gacacctatg
    41701 ttgaagcacg ggatttgctg gaacaaaccc ttactctcct tgagtcttcc cgtcccgtgg
    41761 aactagaccc cagccaagtt gcccaagagc tctcctacct gatcgaagaa gacaaggttc
    41821 agcagattga taccaagatc tttgacaaca gcctcttttt cgctgaggaa ggcatccgca
    41881 gtcacttgat tcgtatcctt gaaaaaggaa aacagaagag ccaagattta gaaactattc
    41941 aaaagcatat cactactgtc gagcaagaac tggggattga gtatgataac attcaaaaac
    42001 aagctatttg tgacgctatc cagaacaagg tctttatcct gacaggtggg cctggtactg
    42061 gtaagacaac tgttatcaat ggaatcattg ctgtttatgc ccttttagaa ggacttgact
    42121 tcaggaagaa aagcaatctg cccattcttc ttgctgctcc aactggtcga gctgctcgtc
    42181 gcatgaatga attgacaggt ttgcctagcg cgaccataca tcgccatttg ggaatgacag
    42241 gtgacgatga taccagtcat ctggaagatt acctagatgc tgactttatc atcgtggatg
    42301 aattctctat ggtagatact tggctggcca atcaactctt ctccaatatc tcttctaaca
    42361 gtaagatcct catcgtaggt gacagtgatc agctaccttc tgtcagtcct ggacaggttc
    42421 tagcggatct acttcatatt cctttgattc ctcagactcg cttggaaaaa atttatcgac
    42481 aaagcaaaga atcaaccatc gtcaccctag ctagtcagat tcgacagggc atcttgccag
    42541 ctgatttcac ccaaaaaaaa gctgaccgtt cctactttga aattgctagt ggccatattc
    42601 ctgccaccat tgaaaaaatc ttaggtgccg ccctcagaaa tggtattccc gcccgtgata
    42661 tccaagttct ggctcctatg taccgaggga cggcagggat tgatgctatc aatcagctca
    42721 tgcaagacct gctcaatccc ccacaaaaag atcaactcag ttttgaagct cctcagtgtc
    42781 actatcgtaa gagagacaag gtcattcatt tggttaacga tgctgaaatc aatgtcttta
    42841 atggggattt aggagctatc acagacctga ttcctggtaa atacaccgaa tcgaaacaag
    42901 acgagattgt cattgatttt gatggtaatg aagtctctta cccccgtaac gaatggtaca
    42961 agattcgctt ggcctatgcc atgagtattc ataagtctca gggaagtgag ttcccagttg
    43021 tcatcctacc gattactagt gctagccgac gtatgctgga gcgaaatctc atctacacag
    43081 ccattacacg tgccaaaagc aaactcatct tactaggcga attacaagcc ttcgactatg
    43141 ctacccaaca catcggaact gcccgaaaaa cctatctgat tgaacgcttc agtgatctac
    43201 tggagaatgt tgaagaaaag caacaagctg tctctgaaac agtcacatca agtgcctctg
    43261 aacaatccta catcctaacc gaagaaaact gggaccgcat cccagccatg attgggatta
    43321 cagacacaga cctcaaagag atttttggaa aatagtgcat cagaaaaccc accaattcag
    43381 gtgggttttc tgtttattaa gattatttta ctgttgctgt gcttgtgatc aattcaacag
    43441 cttttttgat agtgatatca tgtttcaaca tgtcagctga aagcaagttt tgaacttgtg
    43501 caacttccat gttgtagtct gctgccaatt gctcaacttc tttttggatt tcttcttctg
    43561 aagcatcaaa tccttcagct ttggcaactg cttcgataac aaggttagtc ttagtacgtg
    43621 actcagcttc tgcttggtat tggttgtgaa ggtcttcttg agtagttcca gtgatttgga
    43681 agtacatgtc agggttgatc ccttgacgtt gcaaattccc aaggaattca tttactgaac
    43741 ggtgaacttc ttcatggatc atttcttctg gaagttctac gatttcagca ttttctacag
    43801 ctgtatcaat tgctgcacct tcaactgcat ctttgtaagc ttcttcttta gcagcagcca
    43861 attctttgct gtatttttct ttcaagtcag caagtgtttc aacttcttca tcaatgtctt
    43921 ttgcaagttc atcgtcaaga gccggaactt ctttagcttt tacttcgtgg atagttgtca
    43981 cgaatttagc ttctttacct gcaaggtctt ctgcttggta gtcttctggg aatgttacga
    44041 taacatcaac ggtttcgcca gctgagtgac ctaccaattg gtcttcgaaa ccagggatga
    44101 attgacctga accaagtcca agtgagaagt tttcaccttt tccaccgtca aattcaacac
    44161 cgtcgataga accaacgaag tcgatcacaa cagtgtcgcc gttttcagca gcagcttcct
    44221 tgataaccaa ttcagccagg ttgttgcgtt cgcgttcgat acgctcttcg acatcagcgt
    44281 cagttacttc tttttctaca tcaactgata cttcaaggtt tttgtagtca cccaatttta
    44341 cttcaggttt tgtaacgact tcagcagtga taacccagtc ttgacctttt tccattgaag
    44401 ttacgtcaat ttttggttgg gcaaccactt caagaccagc ttcttttaca gctgcttcat
    44461 aagcgtttgg caaaagagcg ttcataacgt cttggtaaag tgattcttca ccaaattttt
    44521 tatcgaagat aggacgtgga aggtgacctt tacggaaacc tggaacatta agagatttct
    44581 tcactgactt gaagacacgg tccaattctg gtttgatttg gtcttgagag atagtgaaag
    44641 tcaagacacc acggtttgtt tctttgtttt caaatgatac agacattctg tcatttctcc
    44701 ttaaaatttt ttaaata
//
LOCUS       DAVKJD010000009        44247 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKJD010000009 DAVKJD010000000
VERSION     DAVKJD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44247
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.009"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            145..2094
                     /gene="mutL"
                     /locus_tag="VW157_000506"
     CDS             145..2094
                     /gene="mutL"
                     /locus_tag="VW157_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9551857.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(2419..2886)
                     /locus_tag="VW157_000507"
     CDS             complement(2419..2886)
                     /locus_tag="VW157_000507"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9551858.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEISLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(2887..4092)
                     /locus_tag="VW157_000508"
     CDS             complement(2887..4092)
                     /locus_tag="VW157_000508"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9551859.1"
                     /translation="MEYRKIQETLEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRIHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(4112..4747)
                     /gene="ribE"
                     /locus_tag="VW157_000509"
     CDS             complement(4112..4747)
                     /gene="ribE"
                     /locus_tag="VW157_000509"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9551860.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSINGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLKQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(4732..5832)
                     /gene="ribD"
                     /locus_tag="VW157_000510"
     CDS             complement(4732..5832)
                     /gene="ribD"
                     /locus_tag="VW157_000510"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9551861.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(5967..6081)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6238..6831
                     /gene="ruvA"
                     /locus_tag="VW157_000511"
     CDS             6238..6831
                     /gene="ruvA"
                     /locus_tag="VW157_000511"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9551862.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLIYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKIAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            6841..7404
                     /locus_tag="VW157_000512"
     CDS             6841..7404
                     /locus_tag="VW157_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9551863.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            7401..8078
                     /locus_tag="VW157_000513"
     CDS             7401..8078
                     /locus_tag="VW157_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9551864.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(8215..9246)
                     /locus_tag="VW157_000514"
     CDS             complement(8215..9246)
                     /locus_tag="VW157_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9551865.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEVLLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(9501..10184)
                     /locus_tag="VW157_000515"
     CDS             complement(9501..10184)
                     /locus_tag="VW157_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9551866.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(10196..11140)
                     /locus_tag="VW157_000516"
     CDS             complement(10196..11140)
                     /locus_tag="VW157_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9551867.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            11272..14103
                     /gene="uvrA"
                     /locus_tag="VW157_000517"
     CDS             11272..14103
                     /gene="uvrA"
                     /locus_tag="VW157_000517"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9551868.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            14096..15157
                     /locus_tag="VW157_000518"
     CDS             14096..15157
                     /locus_tag="VW157_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9551869.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            15289..15687
                     /gene="spx"
                     /locus_tag="VW157_000519"
     CDS             15289..15687
                     /gene="spx"
                     /locus_tag="VW157_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9551870.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            15753..16322
                     /locus_tag="VW157_000520"
     CDS             15753..16322
                     /locus_tag="VW157_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9551871.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPESDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            16409..16675
                     /locus_tag="VW157_000521"
     CDS             16409..16675
                     /locus_tag="VW157_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9551872.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            16679..17098
                     /gene="ruvX"
                     /locus_tag="VW157_000522"
     CDS             16679..17098
                     /gene="ruvX"
                     /locus_tag="VW157_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9551873.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            17114..17419
                     /locus_tag="VW157_000523"
     CDS             17114..17419
                     /locus_tag="VW157_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9551874.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            17775..19025
                     /locus_tag="VW157_000524"
     CDS             17775..19025
                     /locus_tag="VW157_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9551875.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            19022..19564
                     /locus_tag="VW157_000525"
     CDS             19022..19564
                     /locus_tag="VW157_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9551876.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            19710..21242
                     /gene="cls"
                     /locus_tag="VW157_000526"
     CDS             19710..21242
                     /gene="cls"
                     /locus_tag="VW157_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9551877.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            21316..21459
                     /locus_tag="VW157_000527"
     CDS             21316..21459
                     /locus_tag="VW157_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551878.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            21496..23034
                     /locus_tag="VW157_000528"
     CDS             21496..23034
                     /locus_tag="VW157_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9551879.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            23154..25361
                     /gene="nrdD"
                     /locus_tag="VW157_000529"
     CDS             23154..25361
                     /gene="nrdD"
                     /locus_tag="VW157_000529"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9551880.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKGENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            25373..25513
                     /locus_tag="VW157_000530"
     CDS             25373..25513
                     /locus_tag="VW157_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551881.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            25557..26063
                     /locus_tag="VW157_000531"
     CDS             25557..26063
                     /locus_tag="VW157_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9551882.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLGLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            26056..26646
                     /gene="nrdG"
                     /locus_tag="VW157_000532"
     CDS             26056..26646
                     /gene="nrdG"
                     /locus_tag="VW157_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9551883.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            26643..27260
                     /locus_tag="VW157_000533"
     CDS             26643..27260
                     /locus_tag="VW157_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9551884.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            27526..27834
                     /gene="rpsJ"
                     /locus_tag="VW157_000534"
     CDS             27526..27834
                     /gene="rpsJ"
                     /locus_tag="VW157_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9551885.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            28050..28676
                     /gene="rplC"
                     /locus_tag="VW157_000535"
     CDS             28050..28676
                     /gene="rplC"
                     /locus_tag="VW157_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9551886.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            28701..29324
                     /gene="rplD"
                     /locus_tag="VW157_000536"
     CDS             28701..29324
                     /gene="rplD"
                     /locus_tag="VW157_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9551887.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            29324..29620
                     /locus_tag="VW157_000537"
     CDS             29324..29620
                     /locus_tag="VW157_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9551888.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            29638..30471
                     /gene="rplB"
                     /locus_tag="VW157_000538"
     CDS             29638..30471
                     /gene="rplB"
                     /locus_tag="VW157_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9551889.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            30575..30856
                     /gene="rpsS"
                     /locus_tag="VW157_000539"
     CDS             30575..30856
                     /gene="rpsS"
                     /locus_tag="VW157_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9551890.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            30868..31212
                     /gene="rplV"
                     /locus_tag="VW157_000540"
     CDS             30868..31212
                     /gene="rplV"
                     /locus_tag="VW157_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9551891.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            31225..31878
                     /gene="rpsC"
                     /locus_tag="VW157_000541"
     CDS             31225..31878
                     /gene="rpsC"
                     /locus_tag="VW157_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9551892.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            31882..32295
                     /gene="rplP"
                     /locus_tag="VW157_000542"
     CDS             31882..32295
                     /gene="rplP"
                     /locus_tag="VW157_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9551893.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            32305..32511
                     /locus_tag="VW157_000543"
     CDS             32305..32511
                     /locus_tag="VW157_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9551894.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            32536..32796
                     /gene="rpsQ"
                     /locus_tag="VW157_000544"
     CDS             32536..32796
                     /gene="rpsQ"
                     /locus_tag="VW157_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9551895.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            32822..33190
                     /gene="rplN"
                     /locus_tag="VW157_000545"
     CDS             32822..33190
                     /gene="rplN"
                     /locus_tag="VW157_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9551896.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            33268..33573
                     /gene="rplX"
                     /locus_tag="VW157_000546"
     CDS             33268..33573
                     /gene="rplX"
                     /locus_tag="VW157_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9551897.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            33597..34139
                     /gene="rplE"
                     /locus_tag="VW157_000547"
     CDS             33597..34139
                     /gene="rplE"
                     /locus_tag="VW157_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9551898.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            34157..34426
                     /gene="rpsN"
                     /locus_tag="VW157_000548"
     CDS             34157..34426
                     /gene="rpsN"
                     /locus_tag="VW157_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9551899.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            34711..35109
                     /gene="rpsH"
                     /locus_tag="VW157_000549"
     CDS             34711..35109
                     /gene="rpsH"
                     /locus_tag="VW157_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9551900.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            35301..35837
                     /gene="rplF"
                     /locus_tag="VW157_000550"
     CDS             35301..35837
                     /gene="rplF"
                     /locus_tag="VW157_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9551901.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            35921..36277
                     /gene="rplR"
                     /locus_tag="VW157_000551"
     CDS             35921..36277
                     /gene="rplR"
                     /locus_tag="VW157_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9551902.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            36295..36789
                     /gene="rpsE"
                     /locus_tag="VW157_000552"
     CDS             36295..36789
                     /gene="rpsE"
                     /locus_tag="VW157_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9551903.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            36761..36985
                     /gene="rpmD"
                     /locus_tag="VW157_000553"
     CDS             36761..36985
                     /gene="rpmD"
                     /locus_tag="VW157_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9551904.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            37130..37570
                     /gene="rplO"
                     /locus_tag="VW157_000554"
     CDS             37130..37570
                     /gene="rplO"
                     /locus_tag="VW157_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9551905.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            37583..38893
                     /gene="secY"
                     /locus_tag="VW157_000555"
     CDS             37583..38893
                     /gene="secY"
                     /locus_tag="VW157_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9551906.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            39044..39682
                     /locus_tag="VW157_000556"
     CDS             39044..39682
                     /locus_tag="VW157_000556"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9551907.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNSDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            39799..40017
                     /gene="infA"
                     /locus_tag="VW157_000557"
     CDS             39799..40017
                     /gene="infA"
                     /locus_tag="VW157_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9551908.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            40042..40158
                     /gene="rpmJ"
                     /locus_tag="VW157_000558"
     CDS             40042..40158
                     /gene="rpmJ"
                     /locus_tag="VW157_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9551909.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            40176..40541
                     /gene="rpsM"
                     /locus_tag="VW157_000559"
     CDS             40176..40541
                     /gene="rpsM"
                     /locus_tag="VW157_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9551910.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            40559..40942
                     /gene="rpsK"
                     /locus_tag="VW157_000560"
     CDS             40559..40942
                     /gene="rpsK"
                     /locus_tag="VW157_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9551911.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            40985..41920
                     /locus_tag="VW157_000561"
     CDS             40985..41920
                     /locus_tag="VW157_000561"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9551912.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            41932..42318
                     /gene="rplQ"
                     /locus_tag="VW157_000562"
     CDS             41932..42318
                     /gene="rplQ"
                     /locus_tag="VW157_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9551913.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            42583..42849
                     /locus_tag="VW157_000563"
     CDS             42583..42849
                     /locus_tag="VW157_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9551914.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            42859..44196
                     /locus_tag="VW157_000564"
     CDS             42859..44196
                     /locus_tag="VW157_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9551915.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
BASE COUNT        13343 a         8430 c         9733 g        12741 t
ORIGIN      
        1 actattttat agcccatggc aaggaacctt gtctatgtgg agggattggg ctcctatgtg
       61 gtggagcttt tctgttcttt ctgaaatatg gtataatagc actaatcaat ttctaggaaa
      121 atagatacag aaaggggctg aaagatgtct catattattg aattgccaga gatgctggca
      181 aaccaaatcg cggctggaga ggtcattgaa cgtcctgcca gtgtggtcaa agagttggta
      241 gaaaatgcca ttgacgcggg ctctagtcag attatcattg agattgagga agctggtctc
      301 aagaaggttc aaatcacgga taacggtcat ggaattgccc acgatgaggt ggagttggcc
      361 ctgcgtcgcc atgcgaccag taagataaaa aatcaagcag atctctttcg gattcggacg
      421 cttggttttc gtggtgaagc cttgccttct attgcgtctg ttagtgtctt gactctgtta
      481 acggcggtgg atggtgctag tcatggaacc aagttagtcg cgcgtggggg tgaagttgag
      541 gaagtcatcc cagcgactag tcctgtggga accaaggttt gtgtggagga tctctttttc
      601 aacacgcctg cccgtctcaa gtatatgaag agccagcaag cggagttgtc tcatatcatt
      661 gatattgtca accgtctggg cttggcccat cctgagattt cttttagctt gattagtgat
      721 ggcaaggaaa tgacgcggac agcagggact ggtcaattgc gccaagcaat cgcagggatt
      781 tacggtttgg tcagtgccaa gaagatgatt gaaattgaga actctgacct agatttcgaa
      841 atttcaggtt ttgtgtcctt gcctgagttg actcgggcta accgcaatta tatcagcctc
      901 ttcatcaatg gccgttatat taagaacttc ctgctcaatc gtgctatttt ggatggtttt
      961 ggaagcaagc ttatggttgg acgttttcca ctggctgtca ttcacatcca tatcgaccct
     1021 tatctagcgg atgtcaatgt gcatccaact aagcaagagg tgcggatttc caaggaaaaa
     1081 gaactgatga ctctggtttc agaagctatt gcaaatagtc tcaaggaaca aaccttgatt
     1141 ccagatgcct tggaaaatct tgccaaatcg accgtgcgca atcgtgagaa ggtggagcaa
     1201 actattctcc cactcaaaga aaatacgctc tactatgaga aaactgagcc gtcaagacct
     1261 agtcaaactg aagtagctga ttatcaggta gaattgactg atgaagggca ggatttgacc
     1321 ctgtttgcca aggaaacctt ggaccgattg accaagccag caaaactgca ttttgcagag
     1381 agaaagcctg ctaactacga ccagctagac catccagagt tagatcttgc tagcatcgat
     1441 aaggcttatg acaaactgga gcgagaagaa gcatccagct tcccagagtt ggagtttttc
     1501 ggacaaatgc acgggactta tctctttgcc caagggcgag atggacttta catcatagat
     1561 cagcacgctg ctcaggaacg ggtcaagtac gaggagtacc gtgaaagcat tggcaatgtt
     1621 gaccagagcc agcagcaact cctagtgccc tatatctttg aatttcctgc ggatgatgcc
     1681 ctgcgtctca aggaaagaat gcctctctta gaggaagtgg gcgtctttct agcagagtac
     1741 ggagaaaatc aatttattct acgtgaacat cctatttgga tggcagaaga agagattgaa
     1801 tcaggcatct atgagatgtg cgacatgctc cttttgacca aggaagtttc tatcaagaaa
     1861 taccgagcag agctggctat catgatgtct tgcaagcgat ctatcaaggc caatcatcgt
     1921 attgatgatc attcagctag acaactcctc tatcagcttt ctcaatgtga caatccctat
     1981 aactgtcctc acggacgtcc tgttttggtg cattttacca agtcggatat ggaaaagatg
     2041 ttccgacgta ttcaggaaaa tcacaccagt ctccgtgagt tggggaaata ttaaaagtat
     2101 aaaaaagtct gggaaaaatt ttcaaaatca gaaaaacgca taaaatcagg tgttcaaaaa
     2161 ccttgatttt atgcgtttta tcatagaaat atttacttca ttttttccta attcttttcg
     2221 aaactctttt taaacgacgt cagttttatc agtaatctca aaacagtgtt ttgagctaat
     2281 tttgccagtt ttgtctgtaa catcgaagtt gtgtttgacc actctgcgac tggtttccta
     2341 gtttgctcta tgattttcac agagtattaa attgcgattt tgccaagttt ctttattcgt
     2401 ctaaaagtag agtctgttct atgcgtctaa tgtacgaatt aggttgacca tttcaatagc
     2461 tccttgtgca cactcagaac ccttatttcc tgctttagta ccagctcgtt ctatggcttg
     2521 ttcaattgta tctgtcgtta gcacaccaaa cataacagga atttcgctat ttaaactgat
     2581 ttgggcgatt cccttagata cctcgctaca tacataatca taatgacttg tattccctct
     2641 aatgacagct cccaagcaga taattgcatc atatttttta ctttttgcca tttttgatgc
     2701 aatcagtgat atttcaaaag ctcctggaac ccaggctacc tctatatctt tctcgtttac
     2761 attctctctt ttgagattat ctagtgctcc agataataat tttgaagtta taaattcatt
     2821 aaatctcgct acaacaatac ctattttaat attgtttgct actaaattac cttcataagt
     2881 gttcatttat ttttcctcca tatttaaaat gtgacccatt cgatttttct ttgtttctaa
     2941 ataaaaacta tcgtaaggat tggcttctat ttcgattgat attctactgg aaatagtaat
     3001 tccatatttt tctaactgtt caaccttgtc aggattattt gtcagtaaat gaagtgactg
     3061 aagtcccaga tctttaagca tttgtgctcc aatatgatat tctcttaaat caccttcaaa
     3121 gcctaatgca agattggcat caagcgtatc catgccttga tcttgtaaat gataggcttt
     3181 taatttattg ataagtccaa ttcctcgtcc ctcctgtcgc aagtaaagta agacacccga
     3241 accattctca acaatcattt tcatagcttt atcgaattgc tgtccacaat cgcaacgtaa
     3301 agagcctaaa acatcccctg ttaaacattc ggagtggatc cgacataata cattggcttc
     3361 atcctctatg tttcccataa taagagcaag atgatgttcc ccatttagtt tatctatata
     3421 gctaattgct ttgaaattac cgtatctagt aggcatattg acagttgaaa ctcgttctac
     3481 cagctgatca tatacttttc tatattcttg taattctttg atggtaatta gtggaatgtt
     3541 gtgtttcttc gagaactgaa ttaaatcatc tgttctcatc attttgccat catgattcat
     3601 tatttcacaa cataggccac actcttttag tccagctaat tttaataaat caacagttgc
     3661 ttctgtgtgt ccatttcttt ctagaacacc accttttttt gcaattaaag gaaacatgtg
     3721 tcctggcctg cgaaaatcag agggtgttat atcttcagct acacacatac gtgcggtcag
     3781 tcctctttcc tcggcagaaa tacctgtggt cgtttcttta taatcaattg aaactgtaaa
     3841 agcagtctta tgattatctg tattgttttc aaccataggt gaaagcatta attgattagc
     3901 taaactttcg ctcataggca tacaaattaa tcctttggca taagtagcca taaaattaac
     3961 attttctgtt gtagctgctt gtgcagaaca aattaagtct ccttcatttt ctctatcctt
     4021 gtcgtctata acaagaacaa gtcgtccctt ctgcaatgct tctaatgttt cttgtatttt
     4081 tcgatattcc attgactgat tatcctttct gctaaaatcc attttgatat aatagttcct
     4141 tagatatttc tgattttgga gagttatcca tcagtttttg cacatattta cctaagatat
     4201 cattttcaag atttactgta ctcccgactt gtttactttt aagaatggtt tgtttcaagg
     4261 tatgagggat aacagatact gaaaagttta ctttggagac tttagcgaca gtcagactaa
     4321 tgccgtcaat tgtaatagat cctttttcaa ctattaaatc taaaatttct ttttgtgtgt
     4381 tgatttgata ccatacagca ttatcatctt tttttattga cgagattttt cctgtaccat
     4441 caatgtgtcc tgtaacgacg tgacccccaa gtcgaccgtt gatagataag gctctttcta
     4501 gattcacctc acttccatgt tttaatagag taagagctgt tcgactccat gtttcattca
     4561 ttacatcaac tgtaaaggat tgatgattga aatgagtaac tgtaagacag ataccattta
     4621 ctgctatact atcgcctaaa tggatatccg ttaatatttt tgaggcttta attgatagtt
     4681 tacaattacg agagtctttc tgtattcttt caacttttcc gatttcttca attattcctg
     4741 tgaacatgga taaatcactt cactttctat gagatagtca tttcctattt gagaaaaagc
     4801 ataaggtttc aatctaatag cgtcatttgg caaagaaatg ccttcacctc cgacaggaaa
     4861 cttggcactg cctccaaaaa tttttggtgc aatatatatt ttcagctcat caacaatttg
     4921 ttgttccaaa gcactccaat tcattagact gcccccttct agaactaggc tatcaatctg
     4981 catgtttcct agatgttgca ttaaactcga taagtctata tgattgcctt ttttctttat
     5041 ggaaagtatt tcacagccat gattttgata tagcttcatt ttatttttgt cttcagagga
     5101 agtggcaatg taagttttaa tatcatttgc tgtttttacg attttagagg taagaggagt
     5161 tcgtaaatgt gtatcgcata tgatacggat aggatttttc ccttcctcca atctacatgt
     5221 cagcaaagga tcgtcttgaa taacagtatt gactcccacc ataattgcac taacatggtg
     5281 tcgtaactga tgcacatgct ttcttgcttc ttcttcagta atccatttgg attgatttgt
     5341 tttagtggct atttttccat ccattgacat tgcatatttc ataaaaacat agggtacatg
     5401 ctgggtaata tactttctaa aactttttat taagttaaga cactcatttt ctaaaattcc
     5461 aacagtaact tgaagattat tttcctcaag tatctttact ccttttccag atacaatagg
     5521 attacagtct aggcttccaa tgactactct tgtaatacca ctatcgatta tagcatctat
     5581 acagggaggt gttttcccga agtgacaaca gggttcaagt gttacataaa gcgtcgctcc
     5641 gacaggggat tctctacagt ttttaagagc atttctctca gcatgtgggc caccaaaaaa
     5701 ctcatgataa ccttgtccga tgatgtgatt atcttttaca ataactgcgc cgaccatagg
     5761 attgggattg acgtaaccag cccctttttg tgccagtttt attgctaatt tcatatattt
     5821 tgaatcgctc atctcactac ctccaaaaaa aatatacctt gaatagggga ctactcaagg
     5881 catacaaaag aaaacttatg cgattaacaa aaatactctg aaatgacaag taatcatttc
     5941 agagcacgca aaaagcacaa atatactttt atcttctttc atccagacta tactgtcggc
     6001 tttggaattt caccaaatca tgcctttcgg ctcgtgggct ataccaccgg tagggaatta
     6061 caccctgccc tgaagatagt tattcaatta cagatgatta tagtacttaa ttttgaatat
     6121 gtcaacagat aaataccgat tgtttttgat atactgtatt tgtgataatc gattctcgct
     6181 cctcggataa agaaaatatg atatactaga taaacgaaat aagagagaag gaacactatg
     6241 tacgcatatt taaaaggaat cattaccaaa attactgcca aatacattgt tcttgaaacc
     6301 aatggtattg gttatatcct gcatgtggcc aatccttatg cctattcagg tcaggttaat
     6361 caggaggctc agatttatgt gcatcaggtt gtgcgtgagg acgcccattt gatttatgga
     6421 tttcgctcag aggatgagaa aaagctcttt cttagtctga tttcggtctc tgggattggt
     6481 cctgtatcag ctcttgctat tatcgctgct gatgacaatg ctggcttggt tcaagccatt
     6541 gaaaccaaga acatcaccta cttgaccaag ttccctaaaa ttggcaagaa aacagcccag
     6601 cagatggtgc tggacttgga aggcaaggta gtagttgcag gagatgacct tcctgccaag
     6661 atcgcagtgc aagcaagtgc tgaaaaccaa gaattggaag aagctatgga agccatgttg
     6721 gctctgggct acaaggcaac agagctcaag aaaatcaaga aattctttga aggaacgaca
     6781 gatacagctg agaactatat caagtcggcc cttaaaatgt tggtcaaata ggagcagaga
     6841 atgacaaaac gttgttcgtg ggtcaagatg accaacccac tctacatcgc ctatcatgat
     6901 gaggagtggg gccagcccct ccatgatgac caagcgttgt ttgagttgtt gtgtatggaa
     6961 acctatcagg caggcctgtc ttgggaaacg gtactcaaca aacgccaagc tttccgagaa
     7021 gcctttcata gctatcaaat tcactcagtc gcagagatga ctgacactga attggaagcc
     7081 atgctggaga atccagctat cattcgaaat agagccaaga tttttgctac acgcgctaac
     7141 gcccaagcct ttctacagtt acaggcagag tacggctctt ttgatgccta tctttggtct
     7201 tttgttgagg ggaaaactgt cgttaacgat gttcctgatt atcgccaagc cccagctaaa
     7261 acacccttat ctgagaaatt agccaaagat ctcaaaaaac gagacttcaa gttcacaggc
     7321 ccagtcgccg tattgtcttt tctacaggct gcagggctag ttgatgacca cgagaatgat
     7381 tgtgagtgga aaggtcttaa atgatgtcta acaaaaataa gggaattctg atttttgcga
     7441 ttctctatac agtcctcttt gtgtttgatg gcgttaaatt gctggcttct ttaatgccat
     7501 ctgccattgc aaattatctt gtttatgtag ttttagcttt atatggctcc ttcttgttca
     7561 aggatagatt gatccaacaa tggaaggaga ttagaaagac taaaagaaaa ttcttctttg
     7621 gagtcttaac aggatggctc tttctcattc tgatgactgt tgtctttgaa tttgtatcag
     7681 agatgttgaa gcagtttgtg ggactagatg gacaaggtct aaatcagtct aatattcaaa
     7741 gtacctttca agaacaacca ctactgatag ctgtttttgc ttgtgtcatt ggacctctgg
     7801 tagaagaatt atttttccgt caggtcttat tgcattactt gcaggaacgg ttgtcaggtt
     7861 tactaagcat tattctggta ggacttgttt ttgctctgac tcatatgcac agtttggctc
     7921 tatcagagtg gattggtgca gttggttact taggtggagg ccttgccttt tctattattt
     7981 atgtgaaaga aaaagagaat atctactatc ccctacttgt tcacatgtta agcaacagcc
     8041 tctccttaat cattttagct atcagtatag taaaatgaaa tgagaacagg acaaatcgat
     8101 ttctaacaat gttttagaag tagaggtgta ctattctagt ttcaatatac tgtaatatgt
     8161 gatgaaaatg ccagtaatga taccgagaaa aaagctgaga aacttttccc agctttattt
     8221 gttatagtca aagagaatga cttgttcctg tgcatctaca tgagcatgga ccccaaaggg
     8281 tacaattgct cttggagttg cgtggccgac attcagatta tagacaatcg ggatattgct
     8341 gtcaatgata tccaatagta cctctttata gtcgtcatgg aaagtttcat ccataggttt
     8401 tccgaccaag agtccattga tgaccgcgaa tatgccagtg tcctttaaag ttagcaacat
     8461 ctttttgaag tcttctggct taggcttttc ttcgcttgtt tcgagcaaga ggattttccc
     8521 ttcccagtct gacaagtcag ggaaaagttt gtatttttgg cagagttccg tgctatctgc
     8581 gtatcgagag ttgtcaaaga tatcgtagag ggattcgagg caaccaccga ggattttccc
     8641 ctcgaactga gcacttcctt gcaacaagtc aaaacctgta tttgtatgac tgacacgagg
     8701 tgttcccagg gccgtgggac taaaatcagt tcgttcctca taccaaacgt cactagggcg
     8761 gatttctgaa attcttcccg tctcaatcaa ttctttaaag tagtgaaggc tataggctag
     8821 catttctttg tctaattcac aaatgtctgc taaaaaggat tgaccataaa aagtcttgat
     8881 tcctaattta tgcaacatga ggtggttcat ggttgtatcc gagaagccaa gaaaaatttt
     8941 ttgcttgata accttttgga gttggtcatt ttcaaaaaga taaggtagca agcgataggt
     9001 atcgtctcca ccgatggcac ataggatcat gtcgatgcta tcatcagaaa aggcatgaat
     9061 caaatcctct gcacgagctt caggatggtc cttgataaag tctaatcctt ttaacgaatg
     9121 gggcaaaaag atgggattga gtcccagatc cttgagacgt tggataccca agtccacttc
     9181 gtgtttgaca aaatcctctc cgataatgcc actagataaa ctaacaatac caatagtaga
     9241 aaccatatat catcctccta gaaatagatt gagcctattt tatcacaaaa tataagagaa
     9301 agctatgtgg gatttcagaa gaatactttt aatactcttc gaaaatctct tcaaaccacg
     9361 tcagttttat ctgcaacctc aaagctgtgc tttgagcaac ctgcagctag cttcctagtt
     9421 tgctctttga ttttcattga atataactca aaaaagtaag tgaaaaagca accgcacctg
     9481 cagttgcttt tatcgttttc ttattcttga tagcgtgttg gtcctgcact tgcactacgg
     9541 atattcaagc gtttcttgag atagaagcga agggctagga gggctgctcc aataatagct
     9601 agtggcaatg gatccagtac tgggttaagg ctagctggta ggaagcttgt tgcaaagaag
     9661 acaagcaacc aaaggaacat agaagctagg ataactagta cagatttcca gaaaggtgga
     9721 cgttgactgc gatccatatc tggtccatag tattggtaaa caaagtagta catcaagtag
     9781 aaggcaaatc caccaaccag tccaactaat agaagagtaa tcaatccata gccgaaagct
     9841 tggtctgccg caaagaaggt tgtgagggcg ctgacaaggg caaagaggct agtgatgaaa
     9901 agagctgagt ccataatcat cagttttggg tcatcatttt cttttggatg ctctttttcg
     9961 tactgctctt tgacagtgaa gctatgagcc caatgagttg gtgcgccgta tagggaacgg
    10021 gcagttgtac ctttagattg ctcctcaagg atttggggaa tgacttcctc aaaaatagtc
    10081 tggatttcag cgtctgtttt cccatctttg atgaattgtt gggtagcaat gtggacaaac
    10141 tcttggtttt tcttagttaa tttttgtaga tcaatttgag acataggaac tcctcttaga
    10201 accatttttt atggatgaga tagagagtga gcgagacact catagcaaag gcgataaaga
    10261 cgattaacca gaaggcattt ggctctccgt ttagggggat ttcattatcc ttaaagttca
    10321 tcccgtaggc agaaaagacc atggttggga tggacatgac gatggtcaca agggccaagg
    10381 ttttcatgat gttgttctgg ttgttagaaa tgatagaggc aaaggtctct gtcatagaat
    10441 gcaagacgtt tccataaata tctgccatct cgatggcctg ttgggtttca atcagggtgt
    10501 cttcaagcag gtcttcgtcc tcaaggtatt tcttgatatt gctggttgaa ctggtcaatt
    10561 tcttaatcac gcgctcattt gttttgaggg aggccttgaa atagacgata gttttttcca
    10621 attccatgag ctcaatcaat tcttcattac gagttgattg atgcagttga ctttcgattt
    10681 gttcactctt gcggtcgatt gaacgaaggg ctgttaggta aagctctgca ttgcgataaa
    10741 gaatttgaaa gataaaacgt gaacgcatga aggtatagaa attacgcaat cgacggttga
    10801 taaagacatc aaggacaggt agtggttcca aacacgtagt gataatggtt tcctcagtga
    10861 tgataatacc aagcgggatg gttacgtagt aggtgcggtt atttctttcc tccgtgaccg
    10921 gcacgtctac gataatcagg gtatactcgt cttcaatggt aatacgagac atttcttccg
    10981 catcgagcgg tgctcgaagg tcggcaatat caatatcgaa ggtgttggcg atttcgagtg
    11041 attcattttg agtcggattg acgagattga tccaagtacc cggttcaagc gtatcgatct
    11101 ctttaaattc agttgttgta gagagaaaaa cttgtttcat atcccttatc ctttctcatt
    11161 tttcagattt tttcacactg tactattata ctacaaaatc ggccttttgg gtaatagaat
    11221 ctcacttttt ttggtataat gttaagcaat aatggactag aaagaacaaa gatgcaagat
    11281 aaaattgtca ttcatggggc gcgtgcccat aatttaaaaa atattgatgt ggagattccg
    11341 cgagacaagt tggttgtcgt gactggcttg tcaggttcag ggaagtccag tctggctttt
    11401 gataccctct atgcggaggg acaacgtcgc tatgtagaga gtttgtccgc ctacgctcgt
    11461 cagttcttgg gaaatatgga aaaaccagat gtagatgcca ttgatggtct cagcccagct
    11521 atttccatcg accagaaaac gactagtaaa aatcctcgct cgacggtggg aactacgact
    11581 gaaatcaatg actatctgcg tctcctttac gcacgtgtgg ggacgcctta ctgtatcaac
    11641 ggacatggag ctatcaatgc ttcttcggtg gagcaaatcg ttgataaggt tttggagtta
    11701 cctgaacgcc agcgcttgca gatcttggct cctgtcatcc gcaagaaaaa aggccaacat
    11761 aagagtgtta tcgagaaggt tcagaaagac gggtatgttc gtgttcgtgt ggatggggaa
    11821 gtctatgatg tgaccgaagt gccagagttg tctaagagca agcaacacaa tatcgatgtc
    11881 gtggttgacc gtattgttat caaggagggc attcgtagtc gtctctttga ttccattgag
    11941 gctgcccttc gtattgcaga aggttatgtc attatcgaca ccatggacga ttcggagttg
    12001 ctcttctctg agcattatgc ctgtccagtt tgtggtttta ctgtcccaga gttagagcct
    12061 cgtctcttct ctttcaatgc tccttttggc tcttgtagtg agtgtgacgg cttgggcatc
    12121 aagctggagg tggatactga tttggtagtg ccagatgcca gcaaaacctt acgtgaggga
    12181 gcgctagcac cttggaatcc tatctcatcc aactactatc caaacatgtt agagcaggcc
    12241 atgaaagtct ttggagtggc tatggataag ccttttgagg acctgtcaga agaagataag
    12301 aacttgattc tctatgggtc agatggtaag gaattccatt tccactatga gaatgaattt
    12361 ggtggcgtgc gcgatatcga cattccgttt gagggagtta tcaataatat caaacgtcgt
    12421 taccatgaaa ccaatagcga ttacactcgc actcagatgc gtctctacat gaatgagctg
    12481 acctgcggaa cctgtcaagg ctatcgtctc aatgaccagg ccttgtctgt ccgtgtgggc
    12541 ggccagcaag ggccacatat tggagaaatc tcagacctgt ctatcgctga ccacttggac
    12601 ttggtgagcc agttaacttt gtctgaaaat gaagccatca ttgctcgtcc cattctcaag
    12661 gaaatcaagg atcgtttgac cttccttaat aatgtgggtc ttaactatct aaccctgtca
    12721 cgttcagcag gaaccctttc aggtggggaa agtcagcgca ttcgtttggc aacccagatt
    12781 ggttccaacc tatcaggcgt cctctatatc ctagacgagc cgtcaatcgg tcttcaccag
    12841 agggacaatg accgcctgat tgccagtctg aaaaagatgc gtgacttggg caatactctt
    12901 atcgtggtgg aacacgacga agataccatg cgtgaagctg attatctgat tgacgttggt
    12961 cctggtgccg gtgtttttgg tggtgagatt gttgcagcag gtacacctaa gcaagtggct
    13021 cgcaacagca agtctatcac aggtcagtac ttgtcaggca aacgtgtcat cccagtacca
    13081 gaagagcgcc gtgtcggaaa tggtcgtttt attgaagtaa taggagcgcg tgagaacaac
    13141 ttgcaaaatg tcacggctcg ctttccacta ggaaaattta tcgcagtgac aggtgtgtcg
    13201 ggttcaggga aatcgactct aatcaacagc attctcaaaa aagccattgc ccagaagctc
    13261 aaccgtaatt cagacaaacc tggtaagttt aagactatta cagggattga gcatgtcgac
    13321 cgtttgattg atattgacca gagccctatc ggacggacgc cgaggtctaa cccagctacc
    13381 tatacaggag tttttgacga tatacgtgac ctttttgctc agacaaatga agccaagatt
    13441 cgaggctaca agaaggggcg cttcagtttc aacgtcaagg gaggtcgctg tgaagcttgc
    13501 tcaggtgacg ggattatcaa gattgagatg cacttcttgc cagatgttta tgtggcttgt
    13561 gaagtttgcc acggaacccg ctataacagt gaaaccctag aagttcacta caaggaaaag
    13621 aatatttcgc aggtcttgga tatgacggtc aacgatgcgg tagaattttt ccaacacatt
    13681 ccgaaaattc aacgcaaact ccagaccatc aaggatgtag gtctaggcta tgtgaccttg
    13741 ggacagccag ctaccaccct ttctggggga gaagcccaac gtatgaagtt agctagcgaa
    13801 ctccacaaac gctcgacagg aaaatctttc tacattctgg atgagccgac gacagggctt
    13861 catacagagg acattgctcg cctgctcaag gttttagctc gctttgtaga cgatggcaat
    13921 acagtcctcg tcatcgagca caatctggat gttatcaaga cggcagacca tatcattgac
    13981 ttgggacctg agggcggtgt cggtggtgga accatcatcg taacaggaac tccagaagaa
    14041 gtagctgcca acgaagccag ctatacagga cactatttga aaggaaagtt acatcatgaa
    14101 taaacgcgta caagcatttc tagctaaaat gcaagaaaaa gaactagatg gtatcatcat
    14161 caacaatctt aaaaacgtct attatttgac tggtttttgg ggctcaaacg gaacagtctt
    14221 tatcagccgt gaccgtcagg tcttagtgac agactctcgc tatatcatcg cagctaagca
    14281 agaaaccagt ggttttgaga ttgtggctga tcgtgatgaa ttggctgtca ttgcaggaat
    14341 tgttaaggac atgggcttga ctcgtatcgg ttttgaagat gagatttcag tgtcttatta
    14401 ccaccgtatg caggcagctt ttgcaggttt ggacttgctt ccacaaactc agtttgtgga
    14461 aggtcttcgt atgattaagg atgaggcaga gattgcagcg attcgcaagg cttgttctat
    14521 ctcagaccaa gctttccgcg atgcgcttga ctttatcaaa ccaggaaaaa ctgaaattga
    14581 gattgccaac ttccttgatt tccgcatgcg tgagttggga gcatctggct tatcttttga
    14641 tacgatccta gctagcggta tcaattcttc taaaccccat gcccatccaa tgcacaaacc
    14701 agtggagttg ggagaagcca ttaccatgga cttcggctgt ctctatgacc actatgtcag
    14761 tgatatgacc cggactatct atctagggca tgttagcgat gagcaggcag agatttacaa
    14821 tacggttcta aaagctaacc aagccttgat tgaccaagct aaggcaggct taggtttccg
    14881 tgactttgac aaaatccctc gtgatattat cattgaggca ggttatggtg actactttac
    14941 tcacggcatt ggccacggta ttggtctgga tatccatgag gaaccctact ttagccagac
    15001 ttctacagaa actattaaga caggtatggc cttgaccgat gaaccaggta tctatatcga
    15061 aggcaaatat ggcgttcgta tcgaggatga tatcctgatt acagagacag gttgtgaatt
    15121 attgacccta gctccaaaag agttgatagt catttagttg tttgtcaaga aaaaagtacg
    15181 aaaatgacga aaaaagtcaa aaaaaattaa aaataggtcg caagtcggat gttttttatg
    15241 gtataataga ctaaactgat agtaacatgt agcgaaaggg gtaggtacat gattaaaatt
    15301 tatacagtct caagttgtac tagctgtaaa aaagcaaaaa cctggctcaa tgcccaccag
    15361 ttaagttata aagaacaaaa ccttggtaaa gaaggaatta cgagagaaga attactggat
    15421 attctaacca aaacagataa cggaatagcc agcatcgttt cgtctaaaaa tcgctatgcc
    15481 aaagcccttg gagtggatat tgaagatttg agtgtcaatg aagttctcaa tctgattatg
    15541 gaaacaccga gaattttaaa gagcccaatc cttgtagatg aaaaacgcct gcaagttggc
    15601 tacaaggaag acgatattcg tgccttccta ccacgctctg tccgtaatgt agaaaatgca
    15661 gaagcacgtt tgcgtgcagc tctataaaca tcaaggctgg gagcacttcc cagtcttatt
    15721 ctattttaat ttcaaaaaga aagaagaaag aaatgaaaaa aatagttctt gttagtctag
    15781 ctttcctttt tgtcctggtt ggttgcggac agaaaaaaga aactggacca gctacaaaaa
    15841 cagaaaaaga tacgcttcag tcggcattgc cagttattga aaatgctgag aagaatacag
    15901 ttgtaactaa gactttggtc ttgcccgagt cagatgatgg tagccagcag acacaaacaa
    15961 ttacttacaa agacaagact tttttgagtc taactatcca acaaaaacgt ccagtctctg
    16021 atgagttgaa gacttatatt gaccaacatg gagtggagga aactcaaaaa gctcttcttg
    16081 aagcggagga gaaggataag tctatcattg aagctcgtaa attggcaggt ttcaaacttg
    16141 aaacaaaact attgagcgca acggaacttc aaacaacgac tagttttgat tttcaagttc
    16201 tggatgtcaa gaaggcttcc cagttggaac atctgaagaa tattggtttg gaaaatcttt
    16261 tgaaaaatga accaagcaaa tatatttcag atagattggc aaatggcgcg acagaacaat
    16321 agaaaatcca aataaataga ctgcctgaat tgtagaaggt aaggaattat gctataatgg
    16381 tactattcta aggaaagagg gtgttagaat gggatttact gaagaaacag tacgttttaa
    16441 attggacgat tccaataaaa aagaaattag cgaaactttg acagatgttt atgcttcgtt
    16501 gaacgataag ggttacaacc caattaacca aatcgtaggt tacgtattga gtggagaccc
    16561 tgcctacgtt cctcgttata ataatgcacg aaatcaaatc cgtaagtatg agcgtgatga
    16621 aatcgttgag gaattggttc gctactatct caaaggacaa ggagtcgatc tataacctat
    16681 gagaattatg ggattggacg tcggttcaaa aacggtaggg gtggcgatta gcgatccgct
    16741 tggttttaca gctcaagggc ttgaaatcat ccagataaat gaagaacaag gccaatttgg
    16801 ttttgaccgc gttaaggaat tggttgatac ttacaaggtg gaacgatttg tagtgggctt
    16861 gcctaaaaac atgaacaata caagtggacc gcgcgtagaa gctagtcaag cctacggagc
    16921 aaagctagaa gagttttttg gtttaccagt agactatcag gatgaacgct tgacaacagt
    16981 ggctgctgag cgcatgttga ttgaacaagc agatatcagt cgcaataagc gcaagaaagt
    17041 cattgataag ttagcagctc agctgatttt acaaaattat ttagatagaa aattttaata
    17101 taaaggagag gctatgtcac acgatcataa ccacgaccac gaagaacgtg aactaatcac
    17161 actagtagat gagcaaggaa atgaaacctt gtttgaaatc cttttgacaa ttgatggaaa
    17221 agaagaattt ggtaaaaact atgttcttct agtaccagtt aacgcagaag aagacgaaga
    17281 cggacaagtt gaaatccaag cttactcatt catcgaaaac gaagatggaa cagaaggcga
    17341 attgcaacca atcccagaag actcagaaga cgaatggaac atgattgaag aagtcttcaa
    17401 cagctttatg gaggagtaaa aacgtccggg ggacgttttt agccctgcgc tagaaattag
    17461 aaacgcaggt agtctgggag actgtatcag cccaactccc agaaatgggg aagagttgga
    17521 acatccagtg gatgttttta gcccagttcc tttaaataag agagaactgg taggttcggg
    17581 ggacgttttt agcctgacgc taaaaataaa aaccgtcagt aagtccgggg ggcgttttta
    17641 gcccacgttc acattcatag tagctagtta ttagctaacc aggtaagagg tcgggacgaa
    17701 aatcctggcc ttgtttttat tagtaaccat actttaatgc gataattgat tggacttttg
    17761 tcaaggagat gtgtatgttt gaagtagaag aatggctcca tagtcggatt ggtttaaatt
    17821 ttcgatcagg tttgggccga atgcagcaag cggtggattt gttaggaaat cctgagcagt
    17881 cttaccctat tatccacgta acagggacta atgggaaagg atctaccatt gcttttatga
    17941 gggaattatt tatggggcat ggcaaaaaag ttgcgacctt tacctcccct catatcgtct
    18001 ctatcaatga ccgaatctgc attaatgggc aacctatagc agatgcagac tttatccgtt
    18061 tggctgatca ggtaaaggag atggagaaaa cgcttctgca aactcctgac cagttgtcct
    18121 tttttgaatt gctgaccttg gttgcttttc tttattttag ggagcaggag gtggatttgg
    18181 ttttattaga agtgggaatt ggtggcttac ttgacacgac caatgtggta actggagagc
    18241 ttgctgtcat cacctccatt gggcttgacc atcaagaaac cttgggtgat agtctagaag
    18301 caattgcaga gcagaaagct ggtattttca aggctggtaa aaaggcagtg attgcgaaat
    18361 tgcctccaga agctagactt gtctgtcaga aaaaagccga atctttagct gttgaccttt
    18421 atcaggcagg tcaagatttt tcagtgctga atggtgattt ttcaagctct ttactaaatc
    18481 tttcacagct gaacataggc ttagaaggag cctatcagca ggagaatgcg gccttggcgt
    18541 tgcaaacttt tcttcttttt atgggagaaa gaaaggaagc tattgatgaa caggctgtaa
    18601 gaaaggcctt ggaacagacc cattgggctg gtcgcttgga gcgtattcgc ccacagattt
    18661 acttggatgg tgctcataac ctccctgcct tgactcgctt ggttgagttt atcaaagaaa
    18721 aagagcagga aggctatcga cctcaaatcc tctttggatc cttgaaacgt aaggattatc
    18781 aagggatgtt gggttatctg actgaaaatt tacctcaagt ggaactcaaa atgaccggct
    18841 ttgactatca agggtctttg gacgagacag atgtaacagg ttaccatgta attccctctt
    18901 accgagaatt tatcagcagt ttcgaagaaa gggcagacgc tcaagacttg ctgttcgtta
    18961 cagggtctct ctattttatc tcagaagtac ggggctacct gctggaccgt gagcagataa
    19021 attgaccccc ttttttgaac ttgttatact aggggaattg ctggttagag aaaatttctc
    19081 taaattggta gcagaaagga aattcatcat gaaattaaaa agattcacac tttctcttgc
    19141 ttctctagca agttttagtc tcttagtagc ttgttcacaa agagctcaac aggttcaaca
    19201 gcctgttgct cagcaggtcc aacagcctgc tcaacagaat accaatactg caaatgcagg
    19261 aggtaaccaa aatcaagcgg ctccagtaca aaaccaacct gttgctcaac cgaccgatat
    19321 tgatgggact tatactggtc aggatgacgg agaccgtatc actttagtgg taactggaac
    19381 gactggtaca tggactgagc tcgaatctga cggggatcag aaagtcaaac aggttacatt
    19441 tgattcagca aatcaacgca tgattattgg cgatgatgtc aaaatttaca ctgtaaacgg
    19501 taatcaaatc gtcgtagatg atatggatag agacccatcg gaccaaatcg ttttaactaa
    19561 ataagactat gcaagtagaa agggctggat ttttcagcct ttttactttt acctagaaaa
    19621 taatcataaa tacttgcctt ctaccgaata cctgcgttat actagtatca attacttgtt
    19681 tttagcattc aaactatcaa ggaggggata tgaaatatag gaaatttcaa ttattgatgt
    19741 ccaagtatgg ctttagtctt tcgattatgc tacttgaact ttgtcttgtt tttggtctct
    19801 ttctttattt aggacgcatg gctcccattt tatggattac tgtcctcatt ctactgagta
    19861 tcatcacaat catttcgata gtcaaccgta atacgactcc tgagaataag gtaacctggt
    19921 tgttagtagc ctttgtgcca gtatttggtc ccttgctcta tctgatgttt ggtgaaaggc
    19981 gattgtccaa aaaagaaatc aaacaactga agaagctagg ctctatgcat ttccaagaag
    20041 caaatagcca gctactaaaa gagaaattaa aagaaagtga caaggcagct tatggagtca
    20101 tcaagtcctt attgagtatg gataccaatg ctgacatcta tgatcaaact gcctctacat
    20161 tttttcctaa cggagaagct atgtggaaaa agatggtaga agatcttaaa aaggctgaga
    20221 aatttatttt cttggaatat tacattatag aagaaggttt gatgtggaat cgcatactag
    20281 atatactaga gcaaaaggta gctcagggtg tagaggttaa gatgctctat gatgatatcg
    20341 gctgtatggc tactttaaca ggagattatg cacatcgact tcgtcagctg ggcatcgagg
    20401 cccataaatt caataaagtt attcctcgtt tgacagtggc ttataataac agagatcata
    20461 gaaaaatatt gattgttgat ggtcagatag cctatactgg tggggtcaat ctggcagatg
    20521 agtacattaa ccacgtcgag agatttggtt attggaagga tagtggaatt cgcttagacg
    20581 gactagcagt aaaagctctg acacgcttat ttttgaccac ttggtacatt aatcgaggag
    20641 aaattagtga ttttgatcaa tatcatttag aaaatcattc tatcccgagt gacggtttaa
    20701 ccattccata cggaagtgga cccaagccaa tttttcgagc gcaggtaggg aaaaaagttt
    20761 atcagagttt aatcaatcaa gcaacagaat cggtctatat tacgacacct tatttgatta
    20821 tagattatga tttaacagag acaatcaaaa atgcagctat gagaggggtc gatgttcgaa
    20881 ttatcacccc ttacatacca gataagaagt tcattcagtt agtcacgaga ggagcttatc
    20941 ccgaccttct ttctgctggt gttcggattt atgagtatag tccaggtttt attcatagta
    21001 agcagatgtt ggtagacgaa gattttgcgg tggtggggac aatcaatctc gactaccgga
    21061 gcttggtaca ccattatgaa aatgcagtct tactctataa aactccttct ataagggaaa
    21121 tcgcccgaga ttttcgaaat atatttgcag attctcagga agtctatcct cattctatca
    21181 aaacgagctg gtatcaaaag cttgtaaaag aaatcgccca gctattcgcc cctatcttat
    21241 aagaaatcta gaacttagga tacaaaccag acctattttt cttgtctttt acgaatagaa
    21301 agaggagggg aattcatgct aactaaggaa gaagtgaatg atatgattga gttcaaacag
    21361 acacatttac accgctttcg ggaaattgaa acctttgtga aactatgcaa aaaatcactc
    21421 aaacagccat cgcaagctga caatcctagg actttttgat atactaagac agataaattg
    21481 aacaggagaa acgatatgag tgtgtatggt agagtagaag aagttcataa ggagaatcgt
    21541 gaacctctag aataccaaat tgaacaagaa tcgcatcatc gtgaatcaag tcgtcttcct
    21601 ttggtgaaaa ttttactatg gagtacgctt gtaacgggga taactctagg agtaccgcta
    21661 ttactcgact taatgagtgc acaagaagtg caggattttt atgcaggttg ggcgcttcat
    21721 cagacaggga agatttacag cgactattat ggaagtcaag gtttgcttta ttatttgctg
    21781 acttacgtga gtcagggcgg atttttcttt gccatctttg agtggttagc cttggtagca
    21841 ggaggatttt tcctttttag atcagcggac accttgacag agcaaggaga ccaagctgga
    21901 catctggtga ctatttttta catgctagtt acaggtcttg cttttggtgg aggctatgcg
    21961 actcttttag cgcttccttt cttattcgca gcctttagtt tagttgcggc ttacctaagc
    22021 aatccaagcc atgataaggg atttgtacgg attgggctag ctttggcagg cggatttttc
    22081 tttgctccct tatcatcgct cctgtttatt gctgtagtga gtttaggctt gttggtcttt
    22141 aaccttgggc atagacgctt tgcgcatggg ttttatcagt ttcttgcagt ggctttaggt
    22201 ttttcacttg tcttttatcc aactgcctac tatagtgctg caacaggaag ttttggggat
    22261 gcgattagtg gtattcgtta tcctattgac agtattcgct ttgattttac ttctaaaatt
    22321 ttagagaata tgttttttta tggcttgttg tcccttggtt tgggatttgt ggtttgtatc
    22381 tttttaggtc tctttcaatc caaacccttt aaactatatg tcatttcggt tcctgcaagt
    22441 cttgtggtaa ttttaggttt gattttactt ttcttttcac aagagcctct gcacgcttct
    22501 tatttgatgg tcgtcttccc tgttttccta cttttattgg taaccaatat taagagtcaa
    22561 cagagggggc gtagtgctag aagaagccga agagaaacgc cagttagcct atggagtcgt
    22621 ttcttcaaag gaaatctata tctgctagtt tttgggtttg tctatctttt gtctgttcct
    22681 tttttgatga agtttgtcct ttatccagta ccttatcaag aacgtaatcg tcttgctgat
    22741 ttggtaaaag aggagacaaa tacggaagat gctatctatg catgggatga tactgcgact
    22801 ctttatcgta agagtgagcg cttgtcgcca tcggcgattt tgtccccgtt gcactataca
    22861 gcaactgagg aaaatcgtaa taagttactt aatgacttga aagaaaaaca acctaaggtg
    22921 attgtggtaa atgacaaggt ggtagtctgg tctgaagtgg aaacactctt aaaagaaaat
    22981 taccaacaag taaagactga ttactcagag tttaaagtct ataaaattaa ataaccaaat
    23041 caatatcttg tgtattttta aaaattttag gatttttaac acaagatatt gatttttctt
    23101 tttagagtgg tataatactt tttagaaaga acattttaga aaagagcatg cgtatgattg
    23161 cactagaaga aaaaattaca attttgccaa ctctcttcgt cgagaaacga gatgggagac
    23221 gtgttgtatt tgatgtggac aagattgaca aggctctcca caaggcggct gacaaggtta
    23281 tggatgtgac acccctggtt gaaaaacgcc tcaatgatct gactgagcga attattacag
    23341 aaattcatag tcgctttcca cagggaatta agatttacga aattcaaaat atcgtagaac
    23401 atgaactcct tgaagccaaa gaatatgcgc tggctgagga gtatattact tatcggacac
    23461 agagggattt tgagcgctca aaagcgacgg atatcaactt tagtattcat aaacttctca
    23521 acaaagacca gacagttgtc aatgaaaatg ctaataaaga cagtgatgtc tttaacactc
    23581 agcgtgattt gacagcaggg attgttggga aatcaatcgg actgcaaatg cttcctaagc
    23641 acgtagccaa tgcccaccaa aagggggata tccactatca cgatttggac tacagtccct
    23701 atacccctat gaccaactgc tgtttgattg attttaaggg tatgttggaa aatggtttta
    23761 agattggaaa tgcagaggta gagagtccca agtctatcca gactgcgaca gcacagattt
    23821 ctcaaatcat tgccaacgtt gcttctagcc agtacggtgg ctgttcagct gaccgtatcg
    23881 atgaaatttt ggcgccttat gcagagaaga attatcaaaa acatctcaaa gatgcagaag
    23941 agtgggtatt gcctgaaaaa cgggaagatt acgcttggaa gaaaacgcaa aaggacatct
    24001 acgatgccat gcaatctctt gagtatgaaa tcaatactct cttcacttca aatggacaaa
    24061 caccttttac ttcgttaggt tttggtctgg gaaccagtcg ttttgaacga gaaattcaaa
    24121 aagctatttt aaacattcgc atcaagggtc ttggttcaga acaccgtacg gctatctttc
    24181 ctaaacttat tttcactctg aaaagaggcc ttaatttaga ggaaggaact cccaactacg
    24241 acatcaagca gttggctctt gagtgtgcaa ccaaacggat gtatccagat gttttatctt
    24301 acgataagat tgttgacttg acaggatcct tcaaggtgcc tatgggttgc cgttctttcc
    24361 tccaaggatg gaagggtgaa aatggtgtag aagtcaattc aggtcgcatg aatctgggtg
    24421 ttgtgacggt taatctgcct cgtattgctc ttgagtctga aggtgatatg aataagttct
    24481 gggaaatctt caacgagcga atgaatatcg cagaagatgc tcttgtttac cgtgtcgaac
    24541 gcactaaaga ggcgacacca gcaaatgctc ctattcttta tcagtacggt gcttttggcc
    24601 atcgtctagg taaagaagaa agtgttgacc agctctttaa gaatcgtcgt gctaccgttt
    24661 cgctgggcta tatcggcttg tatgaagtag cgacagtttt ctttggtaac agctgggaaa
    24721 gtaatccaga tgctaaggaa ttcacgctag acatcattca cgatatgaaa cgccgtgtag
    24781 aagagtggtc agaccaatat ggctaccatt tctctatcta ctcaacacca tccgaaagtc
    24841 tgacagaccg tttctgccga ctagatatag acaagtttgg ctctattcct gatatcacag
    24901 acaaggaata ctacaccaac tctttccact acgatgttcg taaaaatcca acaccgtttg
    24961 aaaaattgga ctttgagaaa gtctatccag aagcaggtgc gtcaggtggt ttcatccatt
    25021 attgtgagta tccagtcctt cagcaaaatc caaaggcctt ggaagctgtc tgggattatg
    25081 cttatgaccg tgtaggctat ctaggcacca atactccgat tgaccgttgc tacaagtgtg
    25141 actttgaagg ggattttgaa ccaactgaga gagggtttgc ttgtccaaac tgtggcaata
    25201 gcgaccctaa aacagtagat gtggtgaagc gaacttgtgg ctacctaggt aatcctcaag
    25261 caagaccgat ggtcaacggg cgtcacaagg aaatcgctgc gcgtgtcaaa catatgaatg
    25321 gttcaacgat taaaatagct gggcatcaag taacaaatta gaaagaaatg aaatgggaaa
    25381 atatcaacta gacgataagg ggcgcgcaca agtgacccgt tatcacgaga aacactctaa
    25441 aggtggagct ggtaagaaag aacgcttgct tagcttcaga gaacaatttt taaacaagaa
    25501 caagaaaaaa taaaagtgag agccagctct cgcttttctc atagtgggag gtaaggatgg
    25561 aattacgcag accaagatta gcggataaga aagctgtttt agatatgatg acagagtttg
    25621 aaaaatttca gtcgcctcac gacggcggtt tctgggatac agagaacttt gtgtatgaag
    25681 actggttaga aagcaatcag gaacaggaaa tggggattaa tctgcctgaa ggatgggttc
    25741 ctgcaattca gttagtggct ttttctgaga aaggtcaagc agttggattt cttaatctcc
    25801 ggttgggcct cagtaacttt ttactagaag aaggtgggca cattggctac tccatccgtc
    25861 catctgaaag aggcaagggt tatgcaaaag agactctccg tcagggcttg caagttgcta
    25921 aggaaaagaa catcaagaaa gctctggtga cctgtagcgt gaataatcct gctagcagag
    25981 cagtcattct agcaaatggt ggaatatttg aggatgctcg caatggagtc gagcgttatt
    26041 ggatagaggt agcgaatgaa taatccaaaa ccacaagaat ggaaaagcga ggaacttagt
    26101 caaggtcgta tcattgacta caaggccttt aactttgttg atggcgaagg cgtgcgtaac
    26161 tctctctatg tagcaggctg tatgtttcac tgcgaggggt gttataatgt agcgacctgg
    26221 tccttcaatg caggcattcc ctatacagct gagttggaag aacagattat ggcagatctt
    26281 gcccagcctt atgttcaggg gttaacctta ctaggagggg agccttttct taatactggg
    26341 attctcttgc cacttgttaa gcggattcgg aaggaattgc cagacaagga catctggtcc
    26401 tggacgggct acacttggga agaaatgatg ttggaaactc cagataaact ggaactcttg
    26461 tcactgattg acattcttgt cgatggaaga tatgatcgaa ctaagagaaa tcttatgctc
    26521 cagtttcgag gttcgtctaa ccaacgaatt atcgatgtgc aaaaatcgct caaaagtggg
    26581 caagtagtga tttgggacaa gctcaatgac ggaaaagaaa gctatgaaca ggtgaagaga
    26641 gaatgaagaa aaaagactta gtagaccaac tagtctcaga gatcgagacg gggaaagtca
    26701 ggacactggg aatatacggt catggagctt caggtaaatc aacctttgca caggaattgt
    26761 accaagcttt agattctact acagtaaatt tgctagaaac agatccctat atcacctccg
    26821 gacgctattt ggtagtaccc aaggacgcgc cgaatcaaaa ggtgacagcc agtctgccag
    26881 tggcgcatga actggagagt ttgcagagag atatccttgc cttgcaggcg ggtatggatg
    26941 tcttgacaat tgaagaacct tggaaggcta gtgaggtctt gtctggagcc aaaccaattt
    27001 tgattgtcga agggatgtct gttggctttc tacccaagga actctttgaa aaaaccatct
    27061 gtttctacac ggatgaggag accgaattaa agcgacgcct tgctagagat acgactgtga
    27121 gaaatcgcga tgcatccttt atattggcta gccatcagat gagacgggag cagtatctgc
    27181 gatactataa agaaactgag tctaaggcgg atatcttagt ggaccaatca gaagataaat
    27241 ttgatgtcaa gaggacttaa cttatgtata gaagaaatat agaagaaaat ccctaatttt
    27301 agaaagaaaa agtaggaaaa cagtttcatc cccaaaaaac gaaaaaacct aacaaatccc
    27361 ttgcaatcgc aggggatttg tgttattcta ttatggtgct gtaaattaca gccttagttt
    27421 tgatgcaaga ggttgcgaca cgctcggttg cattgccacg caacaccgcg tcggttttct
    27481 tgtggagcta gcctattatc ttaaatagac gaaaaggaga aaaagatggc aaacaaaaaa
    27541 atccgtatcc gtttgaaagc ttacgaacac cgtacgcttg acacagcggc tgcaaaaatc
    27601 gtagaatcag ctactcgtac aggtgcacaa gttgcgggtc caatcccact tccaactgaa
    27661 cgtagcctct acacaatcat tcgtgcgact cacaaataca aagactctcg cgaacaattt
    27721 gaaatgcgta cacacaaacg tttgatcgat atcgttaacc caactcaaaa aacagttgat
    27781 gccttgatga aattggatct tccaagtggt gtaaacgtag aaatcaaact ttaatctaaa
    27841 atataaaaga gcagaggctg gtgtttcaat ctaattgaac acgggctaaa ctcggtgtga
    27901 aaaagataaa cttcctcgcg tctgctagac actgtgtcag tttcctattt tcacgttgag
    27961 tttgacgccc tttgtatctt agacttgagc ataaaaaacg ctcgttaaaa actttttgaa
    28021 taaaaatata gaaaaggaac tattttctca tgacaaaagg aatcttaggg aaaaaagtgg
    28081 gaatgactca aatcttcact gaagctggcg aattgatccc tgtaacagtt attgaagcaa
    28141 ctccaaacgt tgttcttcaa gttaaaactg ttgaaacaga cggatacaac gctatccaag
    28201 ttggtttcga tgacaaacgc gaagtattga gcaacaaacc tgctaaagga catgtagcga
    28261 aagctaacac ggctcctaag cgcttcattc gtgaattcaa aaacgttgaa ggcttggaag
    28321 ttggtgctga aattacagtt gaaacattcg cagctggaga cgttgttgac gtaactggta
    28381 cttctaaagg taaaggtttc caaggtgtta tcaaacgcca cggacaatca cgtggaccaa
    28441 tggctcacgg ttctcgttac caccgtcgtc caggttctat gggacctgtt gcacctaacc
    28501 gcgtattcaa aggtaaaaac cttgcagggc gtatgggtgg cgaccgcgta acaattcaaa
    28561 accttgaagt tgtacaagtt gttccagaaa agaacgttat ccttatcaaa ggtaacgtac
    28621 caggtgctaa gaaatctctt atcactatca aatcagcagt taaagctggt aaataataaa
    28681 gaaaggggaa atcagtcaca atggcaaacg taacattatt tgaccaaact ggtaaagaag
    28741 ctggccaagt tgttcttagc gatgcagtat ttggtatcga accaaatgaa tcagttgtgt
    28801 ttgatgtaat catcagccaa cgcgcaagcc ttcgtcaagg cacacacgct gttaaaaacc
    28861 gctctgcagt atcaggtggt ggacgcaaac catggcgtca aaaaggaact ggacgtgctc
    28921 gtcaaggttc tatccgctca ccacaatggc gtggtggtgg tgttgtcttc ggaccaactc
    28981 cacgttcata cggctacaaa cttccacaaa aagttcgtcg cctagctctt aaatcagttt
    29041 actctgaaaa agttgctgaa aacaaattcg tagctgtaga cgctctttca tttacagctc
    29101 caaaaactgc tgaatttgca aaagttcttg cagcattgag catcgattct aaagttcttg
    29161 ttatccttga agaaggaaat gaattcgcag ctctttcagc tcgtaacctt ccaaacgtga
    29221 aagttgcaac tgctacaact gcaagtgttc ttgacatcgc aaatagcgac aaacttcttg
    29281 tcacacaagc agctatctct aaaatcgagg aggttcttgc ataatgaatt tgtatgatgt
    29341 tatcaaaaaa cctgtcatca ctgaaagctc aatggctcaa cttgaagcag gaaaatatgt
    29401 atttgaagtt gacactcgtg cacacaaact tttgatcaag caagctgttg aagctgcttt
    29461 cgaaggtgtt aaagttgcca atgttaacac aatcaacgta aaaccaaaag ctaaacgtgt
    29521 tggacgttac actggtttta ctaacaaaac taaaaaagct atcatcacac ttacagctga
    29581 ttctaaagca atcgagttgt ttgctgctga agctgaataa tctaaggagg aaatatcgtg
    29641 ggaattcgtg tttataaacc aacaacaaac ggtcgccgta atatgacttc tttggatttc
    29701 gctgaaatca caacaagcac tcctgaaaaa tcattgcttg ttgcattgaa gagcaaggct
    29761 ggtcgtaaca acaacggtcg tatcacagtt cgtcaccaag gtggtggaca caaacgtttt
    29821 taccgtttgg ttgacttcaa acgtaataaa gacaacgttg aagcagttgt taaaacaatc
    29881 gagtacgatc caaaccgttc tgcaaacatc gctcttgtac actacactga cggtgtgaaa
    29941 gcatacatca tcgctccaaa aggtcttgaa gtaggtcaac gtatcgtttc aggtccagaa
    30001 gcagatatca aagtcggaaa cgctcttcca cttgctaaca tcccagttgg tactttgatt
    30061 cacaacatcg agttgaaacc aggtcgtggt ggtgaattgg tacgtgctgc tggtgcatct
    30121 gctcaagtat tgggttctga aggtaaatat gttcttgttc gtcttcaatc aggtgaagtt
    30181 cgtatgattc ttggaacttg ccgtgctaca gttggtgttg tcggaaacga acaacatgga
    30241 cttgtaaacc ttggtaaagc aggacgtagc cgttggaaag gtatccgccc aacagttcgt
    30301 ggttctgtaa tgaaccctaa cgatcaccca cacggtggtg gtgaaggtaa agcaccagtt
    30361 ggtcgtaaag caccatctac tccatggggc aaacctgctc ttggtcttaa aactcgtaac
    30421 aagaaagcga aatctgacaa acttatcgtt cgtcgtcgca acgagaaata atattaaact
    30481 agtcgcttaa gcaactagta aatccgccag ctcggtagcg ctccatagga gtgcaagccg
    30541 ctgtggtaca acatttaaag gagaaaatat aaaaatggga cgcagtctta aaaaaggacc
    30601 tttcgtcgat gagcatttga tgaaaaaagt tgaagctcaa gctaacgacg aaaagaaaaa
    30661 agttattaaa acttggtcac gtcgttcaac gatcttccca agtttcattg gttacactat
    30721 tgcagtttat gacggacgta aacacgtacc tgtttacatc caagaagaca tggtaggcca
    30781 caaacttggt gaatttgcac caactcgtac ttacaaaggt cacgctgcag acgacaagaa
    30841 aacacgtaga aaataaggag aacataaatg gcagaaatta cttcagctaa agcaatggct
    30901 cgtacagtac gtgtttcacc tcgtaaatca cgtcttgttc ttgataacat ccgtggtaaa
    30961 agcgtagccg atgcaatcgc aatcttgaca ttcactccaa acaaagctgc tgaaatcatc
    31021 ttgaaagttt tgaactcagc tgtagctaac gctgaaaaca actttggttt ggataaagct
    31081 aacttggtag tatctgaagc attcgcaaac gaaggaccaa ctatgaaacg tttccgtcca
    31141 cgtgcgaaag gttcagcttc accaatcaac aaacgtacag ctcacatcac tgtagctgtt
    31201 gcagaaaaat aaggaggtaa aatcgtgggt caaaaagtac atccaattgg tatgcgtgtc
    31261 ggcatcatcc gtgattggga tgccaaatgg tatgctgaaa aagaatacgc ggattacctt
    31321 catgaagatc ttgcaatccg taaattcgtt caaaaagaac ttgctgacgc agcagtttca
    31381 actattgaaa tcgaacgcgc agtaaacaaa gttaacgttt cacttcacac tgctaaacca
    31441 ggtatggtta tcggtaaagg tggtgctaac gttgatgcac tccgtgcaaa acttaacaaa
    31501 ttgactggaa aacaagtaca catcaacatc atcgaaatca aacaacctga tttggatgct
    31561 caccttgtag gtgaaggaat tgctcgtcaa ttggagcaac gtgttgcttt ccgtcgtgca
    31621 caaaaacaag caatccaacg tgcaatgcgt gctggagcta aaggaatcaa aactcaagta
    31681 tcaggtcgtt tgaacggtgc agatatcgcc cgtgctgaag gatactctga aggaactgtt
    31741 ccgcttcaca cacttcgtgc agatatcgat tacgcttggg aagaagcaga tactacatac
    31801 ggtaaacttg gtgttaaagt atggatctac cgtggtgaag ttcttccagc tcgtaaaaac
    31861 actaaaggag gtaaataacc aatgttagta cctaaacgtg ttaaacaccg tcgtgagttc
    31921 cgtggaaaaa tgcgcggtga agcaaaaggt ggaaaagaag tagcattcgg tgaatacggt
    31981 cttcaagcta caactagcca ctggatcact aaccgccaaa tcgaagctgc tcgtatcgcc
    32041 atgactcgtt acatgaaacg tggtggtaaa gtttggatta aaatcttccc acacaaatca
    32101 tacactgcta aagctatcgg tgtgcgtatg ggatctggta aaggggcacc tgaaggttgg
    32161 gtagcaccag ttaaacgtgg taaagtgatg ttcgaaatcg ctggtgtatc tgaagagatt
    32221 gcacgtgaag cgcttcgact tgctagccac aaattgccag ttaaatgtaa attcgtaaaa
    32281 cgtgaagcag aataaggaga aggcatgaaa cttaatgaag taaaagaatt tgttaaagaa
    32341 cttcgtggtc tttctcaaga agaactcgcg aagcgcgaaa acgaattgaa aaaagaattg
    32401 tttgaacttc gtttccaagc tgctactggt caattggaac aaacagctcg cttgaaagaa
    32461 gttaaaaaac aaatcgctcg tatcaaaaca gttcaatctg aagcgaaata atagactagg
    32521 gaaggagaaa tttcaatgga acgcaataat cgtaaagttc ttgttggacg tgttgtatct
    32581 gacaaaatgg acaagacaat cacagttgta gttgaaacaa aacgtaacca cccagtctat
    32641 ggtaaacgta ttaactactc taaaaaatac aaagctcatg atgaaaacaa tgttgccaaa
    32701 gaaggcgata tcgtacgtat catggaaact cgcccgcttt cagctacaaa acgtttccgt
    32761 cttgtagaag ttgttgaaga agcggtcatc atctaatcaa acctgaaagg agaaaactga
    32821 aatgattcaa acagaaactc gtttgaaagt cgcagacaac agcggtgctc gcgaaatctt
    32881 gactatcaaa gttcttggtg gttcaggacg taaatttgca aacatcggtg atgttatcgt
    32941 ggcatctgta aaacaagcta ctcctggtgg tgcggttaaa aaaggtgacg ttgttaaagc
    33001 agttatcgtt cgtactaaat caggtgctcg tcgtgctgat ggttcataca tcaaatttga
    33061 cgaaaacgca gcagttatca tccgtgaaga caaaactcct cgcggaacac gtatctttgg
    33121 cccagttgca cgtgaattgc gtgaaggtgg cttcatgaag atcgtgtcac ttgctccaga
    33181 agtactttaa tttttaggaa caaactagtc ccctagcttc aagctagggt gcccttatgg
    33241 gcgtaagaaa aatcaaggag aaacctaatg tttgtaaaaa aaggcgacaa agttcgcgta
    33301 atcgctggta aagataaggg aacagaagct gttgtcctta ctgcccttcc aaaagtaaac
    33361 aaagttatcg ttgaaggtgt taacattgtt aagaaacacc aacgtccaac taacgagctt
    33421 cctcaaggtg gtatcatcga gaaagaagca gctatccacg tatcaaacgt tcaagttttg
    33481 gacaaaaatg gtgtagctgg tcgtgttggt tacaaatttg tagacggtaa aaaagttcgc
    33541 tacaacaaaa aatcaggcga agtgcttgat taatcacgaa ggaaaggaga agtataatgg
    33601 caaatcgttt aaaagaaaaa tatcttaatg aagtagttcc tgctttgaca gaacaattca
    33661 actactcatc agtgatggct gtgcctaaag tagataagat tgttttgaac atgggtgttg
    33721 gtgaagctgt atcaaacgct aaaagccttg aaaaagctgc tgaagaattg gcacttatct
    33781 caggtcaaaa accacttatc actaaagcta aaaaatcaat cgccggcttc cgtcttcgtg
    33841 aaggtgttgc gatcggtgca aaagttaccc ttcgtggtga acgtatgtac gaattcttgg
    33901 ataaattggt atcagtttca cttccacgtg tacgtgactt ccacggtgtt ccaacaaaat
    33961 catttgatgg acgcgggaac tacacacttg gtgtgaaaga acaattaatc ttcccagaaa
    34021 tcaacttcga tgacgttgac aaaactcgtg gtcttgacat cgttatcgta acaactgcta
    34081 acactgacga agagtcacgt gcattgctta caggccttgg aatgcctttt gcaaaataat
    34141 ataggaggta aatctaatgg ctaaaaaatc aatggtagct agagaggcta aacgccaaaa
    34201 aattgttgac cgttatgctg aaaaacgtgc tgcattaaag gcggcagggg actacgaagg
    34261 tttatctaaa ttacctcgca acgcctcacc gactcgttta cataatcgtt gtagagttac
    34321 ggggcgccca cattcagttt accgcaaatt tggtctgagt cgtatcgctt ttcgcgaact
    34381 tgcgcataaa ggtcaaattc ctggtgtaac aaaagcatct tggtaattta agatatcaag
    34441 agcgtcaaaa ctccaagtaa aaataggaaa cttgacgaag aaactaaagt ttctaggaaa
    34501 gtttatcttt ttcacacaga gtttagcccg tgttcaattg ggcttgccaa tttgaacacg
    34561 agctacagct ttggcaaaaa agaccaattt gctttggagc attgcttctg cattaaattg
    34621 tctatttttg ctcgtgctgt tacgctcttt gtatcatgta ttaactagca agtgcaactt
    34681 gcaaactact agtaagagga gaaaaacaaa atggttatga ctgacccaat cgcagacttc
    34741 ctaactcgta ttcgtaatgc taaccaagct aaacacgaag tacttgaagt acctgcatca
    34801 aacatcaaaa aagggattgc tgaaatcctt aaacgcgaag gttttgtaaa aaacgttgaa
    34861 atcatcgaag atgacaaaca aggcgtcatc cgtgtatttc ttaaatacgg accaaatggt
    34921 gaaaaagtta tcactaactt gaaacgtgtt tctaaaccag gacttcgtgt ctacaaaaaa
    34981 cgtgaagacc ttccaaaagt tcttaacgga cttggaattg ccatcctttc aacttctgaa
    35041 ggtttgctta ctgataaaga agcacgccaa aagaatgttg gtggtgaggt tatcgcttac
    35101 gtttggtaaa atcaagatac aaagctcgta aagaacaaag caaaattagg aagttggaga
    35161 agtttgttta caaacaagcc aacttatcta ttttgcacag ttcttagagc gtgttcagtt
    35221 cagctcttga actaaataag tatctgaacc ccgtgaaaac tggccgttct ggcctgacaa
    35281 tttaacagga gaaaataaac atgtcacgta ttggtaataa agttatcgtg ttgcctgctg
    35341 gtgttgaact cgctaacaat gacaacgttg taactgtaaa aggacctaaa ggagaactta
    35401 ctcgtgagtt ctcaaaagat attgaaatcc gtgtggaagg tactgaaata actcttcacc
    35461 gtccaaacga ttcaaaagaa atgaaaacta tccacggaac tactcgtgcc cttttgaaca
    35521 acatggttgt tggtgtatca gaaggattca agaaagaact tgaaatgcgt ggggttggtt
    35581 accgtgcaca gcttcaagga tctaaacttg ttttggctgt tggtaaatct catccagacg
    35641 aagttgaagc tccagaagga attacttttg aacttccaaa cccaacaaca atcgttgtta
    35701 gcggaatttc aaaagaagta gttggtcaaa cagctgctta cgtacgtagc cttcgttcac
    35761 cagaaccata taaaggtaaa ggtatccgtt acgttggtga attcgttcgc cgtaaagaag
    35821 gtaaaacagg taaataatgt tgagtggttg atcatcaacc accaacctat tttccaactt
    35881 tgtgcatagc acacgattta aaactaaaga ggtgaaaact gtgatttcaa aaccagataa
    35941 aaacaaactc cgccaaaaac gccaccgtcg cgttcgcgga aaactctctg gaactgctga
    36001 tcgcccacgt ttgaacgtat tccgttctaa tacaggcatc tacgctcaag tgattgatga
    36061 cgtagcgggt gtaacgctcg caagtgcttc aactcttgat aaagaagttt caaaaggaac
    36121 taaaactgaa caagccgttg ctgtcggtaa actcgttgca gaacgtgcaa acgctaaagg
    36181 tatttcagaa gtggtgttcg accgcggtgg atatctatat cacggacgtg tgaaagcttt
    36241 ggctgatgca gctcgtgaaa acggattgaa attctaatag gaggacacta gaaaatggca
    36301 tttaaagaca atgcagttga attagaagaa cgcgtagttg ctgtcaaccg tgttacaaaa
    36361 gttgttaaag gtggacgtcg tcttcgtttc gcagctcttg ttgttgttgg tgaccacaat
    36421 ggtcgcgtag gatttggtac tggtaaagct caagaagttc cagaagcaat ccgtaaagca
    36481 gtagatgatg ctaagaaaaa cttgatcgaa gttcctatgg ttggaacaac aatcccacac
    36541 gaagttcttt cagaattcgg tggagctaaa gtattgttga aacctgctgt agaaggttct
    36601 ggagttgccg ctggtggtgc agttcgtgcc gttgtggaat tggcaggtgt ggcagatatt
    36661 acatctaaat cacttggttc taacactcca atcaacattg ttcgtgcaac tgttgaaggt
    36721 ttgaaacaat tgaaacgcgc tgaagaaatt gctgcccttc gtggtatttc agtttctgat
    36781 ttggcataag aaaggggata aaatggctca aattaaaatt actttgacta agtctccaat
    36841 cggacgcatt ccatcacaac gtaaaactgt tgtagcactt ggacttggca aattgaacag
    36901 ctctgttatt aaagaagata acgctgctat ccgtggtatg attacagcag tatctcactt
    36961 agtaacagtt gaagaagtaa actaatgaag ttttagggga tgtgcactgt accatcccct
    37021 aaaactagat atagtcatct atgatgacat cgtataggcg agttgatggg ggagacaacc
    37081 ttttctccct tatcggcgct agcattttac aaaagaggag aaaataaaaa tgaaacttca
    37141 tgaattgaaa cctgcagaag gttctcgtaa agtacgtaac cgcgttggtc gtggtacttc
    37201 atcaggtaac ggtaaaacat ctggtcgtgg tcaaaaaggt caaaaagctc gtagcggtgg
    37261 cggagttcgc cttggttttg aaggtggaca aactccattg ttccgtcgtc ttccaaaacg
    37321 tggattcact aacatcaacg ctaaagaata cgcaattgtg aaccttgacc aattgaacgt
    37381 ctttgaagat ggtgctgaag taactccagt tgttcttatc gaagcaggaa ttgttaaagc
    37441 tgaaaagtca ggtattaaaa ttcttggtaa cggtgagttg actaagaaat tgactgtgaa
    37501 agcagctaaa ttctctaaat cagctgaaga agctatcact gctaaaggtg gttcagtaga
    37561 agtcatctaa gagaggtgac ctatgttttt taaattatta agagaagctc ttaaagtcaa
    37621 gcaggttcga tcaaaaattt tatttacaat ttttatcgtt ttggtctttc gtatcggaac
    37681 tagcattaca gttcctggtg tgaatgccaa tagcttgaat gctttaagtg gattatcctt
    37741 cttaaacatg ttgagcttgg tgtcggggaa tgccctaaaa aacttttcga tttttgccct
    37801 aggagttagt ccctatatca ccgcttctat tgttgtccaa ctcttgcaaa tggatatttt
    37861 acccaagttt gtagagtggg gtaaacaagg ggaagtaggt cgaagaaaat tgaatcaagc
    37921 tactcgttat attgctctag ttctcgcttt tgtgcaatct atcgggatta cagctggttt
    37981 taataccttg gctggagctc aattgattaa aactgcttta actccacaag tttttctgac
    38041 gattggtatc atcttaacag ctggtagtat gattgtcact tggttgggtg agcaaattac
    38101 agataaggga tacggaaacg gtgtttccat gattatcttt gccgggattg tttcctcaat
    38161 tccagagatg attcagggca tctatgtgga ctactttgtg aacgtcccaa gtagccgtat
    38221 cacttcatct atcattttcg taatcatttt gattattact gtattgttga ttatttactt
    38281 tacaacttat gttcaacaag cagaatacaa aattccaatc caatatacta aggttgcaca
    38341 aggtgctcca tctagctctt accttccgtt aaaggtaaac cctgctggag ttatccctgt
    38401 tatctttgcc agttcgatta ctgcagcgcc tgcggctatt cttcagtttt tgagtgccac
    38461 aggtcatgat tgggcttggg taagggtagc acaagagatg ttggcaacta cttctccaac
    38521 tggtattgcc atgtatgctt tgttgattat tctctttaca ttcttctata cgtttgtaca
    38581 gattaatcct gaaaaagcag cagagagcct acaaaagagt ggtgcctata tccatggagt
    38641 tcgtcctggt aaaggtacag aagaatatat gtctaaactt cttcgtcgtc ttgcaactgt
    38701 tggttccctc ttccttggtg tgatttccat tttaccgatt gcagctaaag atgtatttgg
    38761 tctttctgat gttgttgcct ttggtggaac aagtctcttg atcattatct ctacaggtat
    38821 cgaaggaatc aagcaattgg aaggttacct attgaaacgt aagtatgttg gtttcatgga
    38881 cagaacagaa taaaagtatt tactgaatca gtaaatgctg agggagtgga ggtttaaact
    38941 ctgacatttg taagagttgg atctcccctc ttctattttg tttttaaata ggggtgaaaa
    39001 gactttttgc ttctatttaa aaataaaata aggagatcaa atcatgaatc ttttgattat
    39061 gggcttacct ggtgcaggta agggaactca agcagcaaaa atcgtagaac aattccatgt
    39121 tgcacatatc tcaacaggtg atatgttccg cgctgcaatg gcaaatcaaa ctgaaatggg
    39181 tgttcttgct aagtcatata ttgacaaggg tgaattggtt cctgacgaag ttacaaatgg
    39241 aatcgtaaaa gaacgccttt cacaagatga tattaaagaa acaggattct tattggatgg
    39301 ttacccacgt acaattgaac aagctcatgc cttggacaaa acattggctg aacttggcat
    39361 tgaactagaa ggtgttatca atattgaagt gaactctgac agcctcttgg aacgtttgag
    39421 tggccgtatc atccaccgcg taactggaga aactttccac aaggtcttta acccaccagt
    39481 tgactataaa gaagaagatt actaccaacg tgaagatgat aagcctgaga cagtaaaacg
    39541 tcgtttggat gttaatattg ctcaaggaga accaatcatt gctcactacc gtgccaaagg
    39601 tttggttcat gacatcgaag gtaatcaaga tatcaatgat gtcttctcag atattgaaaa
    39661 agtattgaca aatttgaaat aaagcgtttt tcatacttgc aaaaattcgc tataaatgtt
    39721 atactgagat agtctgactt ataattgttg tctctgtgtc tagaggcatc gaatcgaaat
    39781 ttatggaggt gcttttgcgt ggcaaaagac gatgtgattg aagttgaagg caaagtagtt
    39841 gatacaatgc cgaatgcaat gtttacggtt gaacttgaaa atggacatca gattttagca
    39901 acagtttctg gtaaaattcg taaaaactat attcgtattt tagcgggaga tcgtgttact
    39961 gtcgaaatga gtccatatga cttgacacgt ggacgtatca cttaccgctt taaataatcg
    40021 aaaaacttgg agggataaga aatgaaagta agaccatcgg tcaaaccaat ttgcgaatac
    40081 tgtaaagtta ttcgtcgtaa tggtcgtgtt atggtaattt gcccagcaaa tccaaaacac
    40141 aaacaacgtc aaggataaga tagaaaggag aaaacatggc tcgtattgct ggagttgata
    40201 ttccaaatga caaacgcgta gtaatctcat tgacttatgt ttatggtatc ggacttgcaa
    40261 catctaagaa aattttggct gctgctggaa tctcagaaga tgttcgtgta cgtgatctta
    40321 catcagatca agaagatgct atccgtcgtg aagtggatgc aatcaaagtt gaaggtgacc
    40381 ttcgtcgtga agtaaacttg aacatcaaac gtttgatgga aatcggttca taccgtggta
    40441 tccgtcaccg tcgtggactt cctgtccgtg gacaaaatac taaaaacaac gctcgcactc
    40501 gtaaaggtaa agctgttgcg attgctggta agaaaaaata atataggagg taaaagtctt
    40561 ggctaaacca acacgtaaac gtcgtgtgaa aaagaatatc gaatctggta ttgctcatat
    40621 tcacgctaca tttaataaca ctattgttat gattactgat gtgcatggta atgcaattgc
    40681 ttggtcatca gctggtgctc ttggtttcaa aggttctcgt aaatctacac cattcgctgc
    40741 tcaaatggct tctgaagctg ctgctaaatc tgcacaagaa cacggtctta aatcagttga
    40801 agttactgta aaaggtccag gttctggtcg tgagtcagct attcgtgcgc ttgctgccgc
    40861 tggtcttgaa gtaacagcaa ttcgtgatgt gactccagtg ccacacaatg gtgctcgtcc
    40921 tccaaaacgt cgccgtgtat aatcatcgca ttacactgct tttcgtttaa gagggagtaa
    40981 ctaaatgatc gagtttgaaa aaccaaatat aacaaaaatt gatgaaaata aagattatgg
    41041 caagtttgta atcgaaccac ttgaacgtgg ctacggtaca actcttggta actctcttcg
    41101 tcgtgtactt ctagcttctc taccaggagc agctgtgaca tctatcaaca ttgatggtgt
    41161 gttacatgag tttgacacag ttccaggtgt tcgtgaagac gtgatgcaaa tcattctgaa
    41221 cattaaagga attgcagtga aatcgtacgt tgaagacgaa aaaatcatcg aactggatgt
    41281 tgaaggtcct gctgaagtaa cagctggtga cattttgaca gatagcgata ttgaaattgt
    41341 aaatccagat cattatctct ttacaatcgg tgaaggttct tctctaaaag cgactatgac
    41401 tgttaacagt ggtcgtggat atgtacctgc tgatgaaaat aaaaaggata atgcaccagt
    41461 tggaacactt gctgtagatt ctatttatac accagttaca aaagtcaact atcaagtgga
    41521 acctgctcgt gtaggtagca atgatggttt cgacaaatta acccttgaaa tcttgacaaa
    41581 tggaacaatt attccagaag atgctttagg gctttcagca cgtattttga cagaacatct
    41641 tgatttgttt acaaatctta ctgagattgc taagtcaact gaagtgatga aagaagctga
    41701 tactgaatct gacgaccgta ttttagatcg tacgattgag gaactggact tgtctgtgcg
    41761 ttcatacaac tgtttaaaac gtgccggtat caatactgtg catgatttga cagaaaaatc
    41821 tgaagcagag atgatgaaag tacgaaatct tggacgcaag agtttggaag aagtgaaact
    41881 caaactcatt gatttgggtc ttggattaaa agataaataa aggaggaata catggcttac
    41941 cgtaaactag gacgcactag ctcacaacgt aaagcaatgc ttcgcgattt gacaactgac
    42001 cttttgatca acgaatcaat cgtgacaact gaagctcgtg ctaaagaaat ccgtaaaact
    42061 gttgaaaaaa tgattactct aggtaaacgt ggtgatttgc atgcacgtcg tcaagcagct
    42121 gctttcgtac gtaatgaaat cgcatctgaa aactatgatg aagcaactga taagtacact
    42181 tctactacag cacttcaaaa attgttctca gaaatcgcac ctcgttatgc tgaacgtaac
    42241 ggtggataca ctcgtatcct taaaactgaa ccacgtcgtg gtgatgcagc gccaatggcg
    42301 atcatcgaat tagtataaaa tcatcaattt tgttgagtgt tatgatgatg gagtcttgtg
    42361 ctcttagtct agctctggtc taccgctagg atttcggtcc tagcgggaac actcatcata
    42421 agttgggata gtagacgctt gtttacgaaa ttgttttttt cttaagaaca acttcgtaag
    42481 caggcgtttt tgagtatttt cgttagaatt atgctatact atttgaaaag aatcctgttt
    42541 aatgttaagg tttcttattt taagaagaat tggagtttac ttatgaaagc cattataact
    42601 gttgttggta aagataaatc tggaattgtt gcaggtgttt ctggtaaaat tgcagaatta
    42661 ggattgaata ttgacgatat ctctcaaact gtcttggatg aatattttac gatgatggct
    42721 gttgtatcta gtgatgaaaa gcaagatttt acctatcttc gtaatgaatt tgaagctttt
    42781 gggcaaactt tgaatgtaaa aatcaatatt cagagtgcag cgattttcga agctatgtat
    42841 aatatctagg aggtcatcat ggatattaga caagttactg aaaccatcgc catgattgag
    42901 gagcaaaact tcgatattag aaccattacc atggggattt ctcttttgga ctgtatcgat
    42961 ccagatatca atcgtgctgc ggagaaaatc tatcaaaaaa ttacgacaaa ggcggctaat
    43021 ttagtagctg ttggtgatga aattgcggct gagttgggaa ttcctatcgt taataagcgt
    43081 gtatcggtga cacctatttc tctgattggg gcagcgacag atgcgacgga ctacgtggtt
    43141 ctggcaaaag cgcttgataa ggctgcgaaa gagattggtg tggactttat tggtggtttt
    43201 tctgccttag tacaaaaagg ttatcaaaag ggagatgaga ttctcatcaa ttccattcct
    43261 cgcgctttgg ctgagacgga taaggtctgc tcgtcagtca atatcggctc aaccaagtct
    43321 ggtattaata tgacggctgt ggcagatatg ggacgaatta tcaaggaaac agcaaatctt
    43381 tcagatatgg gagtggccaa gttggttgta ttcgctaatg ctgttgagga caatccattt
    43441 atggcgggtg cctttcatgg tgttggggaa gcagatgtta tcatcaatgt cggagtttct
    43501 ggtcctggtg tggtgaaacg tgctttggaa aaagttcgtg gacagagctt tgatgtagta
    43561 gccgaaacag ttaagaaaac tgcctttaaa atcactcgta tcggtcaatt ggttggtcaa
    43621 atggccagtg agagactggg tgtggagttt ggtattgtgg acttgagttt ggcaccaacc
    43681 cctgcggttg gagactctgt ggcacgtgtc cttgaggaaa tggggctaga aacagttggc
    43741 acgcatggaa cgacggctgc cttggccctc ttgaacgacc aagttaaaaa gggtggagtg
    43801 atggcctgca accaagtcgg tggtttatct ggcgccttta tccctgtttc tgaggatgaa
    43861 ggaatgattg ctgcagtgca aaatggctct cttaatttag aaaaactaga agctatgacg
    43921 gctatctgtt ctgttggatt ggatatgatt gccatcccag aagatacgcc tgctgaaacc
    43981 attgcggcta tgattgcgga tgaagcagca atcggtgtta tcaacatgaa aacaacagct
    44041 gttcgtatca ttcccaaagg aaaagaaggc gatatgattg agtttggtgg tctattagga
    44101 actgcacccg ttatgaaggt taatggggct tcgtctgtcg acttcatctc tcgcggtgga
    44161 caaatcccag caccaattca tagttttaaa aattaagaaa ataggagaaa ttttaagttc
    44221 tatttaagat tagacgtgta tactata
//
LOCUS       DAVKJD010000010        41882 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKJD010000010 DAVKJD010000000
VERSION     DAVKJD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41882
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.010"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..71
                     /locus_tag="VW157_000565"
     CDS             <1..71
                     /locus_tag="VW157_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEU9551916.1"
                     /translation="IPAMSAPVFDNIEAAIKEHGLS"
     gene            119..1594
                     /locus_tag="VW157_000566"
     CDS             119..1594
                     /locus_tag="VW157_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9551917.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLLIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            1620..1697
                     /locus_tag="VW157_000567"
                     /pseudo
     CDS             1620..1697
                     /locus_tag="VW157_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            1899..8600
                     /locus_tag="VW157_000568"
     CDS             1899..8600
                     /locus_tag="VW157_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU9551918.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAIRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTANVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLSVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIEAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(8848..9705)
                     /locus_tag="VW157_000569"
                     /pseudo
     CDS             complement(8848..9705)
                     /locus_tag="VW157_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9917..10276)
                     /locus_tag="VW157_000570"
     CDS             complement(9917..10276)
                     /locus_tag="VW157_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9551919.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLVMYIEMIVELFLMK"
     gene            10368..11324
                     /locus_tag="VW157_000571"
                     /pseudo
     CDS             10368..11324
                     /locus_tag="VW157_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11308..11865
                     /locus_tag="VW157_000572"
     CDS             11308..11865
                     /locus_tag="VW157_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9551920.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVSQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            11858..12112
                     /locus_tag="VW157_000573"
     CDS             11858..12112
                     /locus_tag="VW157_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551921.1"
                     /translation="MDKQYLREKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12124..14178
                     /locus_tag="VW157_000574"
     CDS             12124..14178
                     /locus_tag="VW157_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9551922.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFRESEEADITAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWLSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHSFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VNFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLGSLVALTFVLFAIRFVMIYGYY
                     AYRTRRLKKKLNKYMKDMLLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEQEESKDYLMHIAILNEVTLELEKELEGTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     ERGINRKFASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVITRIKPNNID
                     EMLRGYYLERKLIFEYEEKRLLTTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            14357..15217
                     /locus_tag="VW157_000575"
     CDS             14357..15217
                     /locus_tag="VW157_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9551923.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFVKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMVSLLVSFGLQLLFAFALFLSLFGQMIL
                     DLLAHYWTKDGIIYQALQGLAGPLTYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILVLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASVQ
                     SLKDPSFNKD"
     gene            15428..16138
                     /gene="ccdA2"
                     /locus_tag="VW157_000576"
     CDS             15428..16138
                     /gene="ccdA2"
                     /locus_tag="VW157_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9551924.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYAGVLLDDKDGAQAS
                     SGKFSISVASLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYVTGAVIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQHGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALASSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16159..16725
                     /locus_tag="VW157_000577"
     CDS             16159..16725
                     /locus_tag="VW157_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU9551925.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSMTSEHQMKDEMKMEQTASKI
                     SAAKGKEVADFELTGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKDAG
                     DDYVVLTVVSPGHKGEQSEEDFKNWYKGLDYKNFPVLVDPSGKLLETYGVRSYPTQAF
                     IDGEGKLVKTQPGFMEKDAILQTLKELA"
     gene            16736..17848
                     /gene="msrB"
                     /locus_tag="VW157_000578"
     CDS             16736..17848
                     /gene="msrB"
                     /locus_tag="VW157_000578"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9551926.1"
                     /translation="MNDKLKIILLIGVFFLAITGFYFLLMRNAGQTDASQIEKAAVSQ
                     GGKTVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDANQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKESLQNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQKNQTERAFSNRYWDKFESGIYVDVATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVATYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            17919..18656
                     /locus_tag="VW157_000579"
     CDS             17919..18656
                     /locus_tag="VW157_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9551927.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPQLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEERLQDLLTDKFEGNMAQKIQSHMADSQFSL
                     KALASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEGR"
     gene            18653..20344
                     /locus_tag="VW157_000580"
     CDS             18653..20344
                     /locus_tag="VW157_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9551928.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLAVVGTFYYQSSSSAIETSIEGN
                     SQTTISQTSHFIQSYIKKLETTSTSLTQQTDILAYAENPSQDKVKGIRDLFLTILKAD
                     QDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELLDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYGSSSEMEAMKPYIETGQGYTPSHKSYVSQEKIAGTDWTVLGVSSLEKLDKVRSQL
                     MWTLLGASVTSLLACLCLVWFSLKRWIAPLKDLRETMLEIASGGQNLRAKETGAYELR
                     EVTRQFNAMLDQVDQLMADVRRQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            20460..21023
                     /locus_tag="VW157_000581"
     CDS             20460..21023
                     /locus_tag="VW157_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9551929.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALTSRPKLIGGARAHYPQDAIRKF"
     gene            21045..26642
                     /locus_tag="VW157_000582"
     CDS             21045..26642
                     /locus_tag="VW157_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9551930.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEEASAKLGEVTVVEAENPQVTINQEQAKAENQAVETEEAPKTEESPKEEPKSEIK
                     PTDDTLPKVEEGKEASAEPATVEEVGGEVESKPEEKVAVKPESQPSDKPAEESKVEQA
                     GEPVAPREDEKAPVEPEKQPEAPEEEKAVEETPKQEESTPDTKAEETVEPKEETKTAK
                     GTQEEGKEGQAPVQEVNPEYKVTTGTVEKSTESELDFTIEVVPDDTKYVDEEVVERQG
                     SKGVQITKTTYETVEGVKTDKVLSTTTEVKTPAVPKVVKKGTKPVEGTTVETREEVIP
                     FETKEQEDDTLKRGTRQVVQKGVEGKKQITETYKTIRGEKTNEAPIVKETVIEQPQDE
                     IIKKGTKGLEKPTLEWVKTDKDVLKKSATASYTLNKPDGVEIKSIKVALKDNTGTVIK
                     EVTVEENNLNATLDNLKYYQGYTLSTTMVYNRGEGEETEMLEDKEVQLDLKKVEIKNI
                     KETSLMSVDDAGVETDKSLLTEKPTDVAPLYLRVTTHDNKTTRLAVSSVEEVVVDGKT
                     LYKVVAKAPDLVQRREDDTLSEEYVHYFEKQLPKVDNVYYSFNELITDMQKNPTGTFK
                     LGADLNAANTPTPSKSYVTGEFKGKLSSVDGQHYTIHNTARPLFNNIVGGTVKNINLN
                     NVNIDMPWADQIASVANVIKGGSLIENVKVKGNVLGRNWVSGFIDKIDSGGRLVNVAF
                     IGNVTSVGDGDKFLTGIVGENWKGYVEKAYVEANIKGKRAKAAGISYWSQNSGDNNAV
                     GRDGAIKKSVVKGSIDVEKPIEVGGAVGSLNYLGYIEDTVAMMKVKNGEIFYGSHDID
                     TDPYYTGERVNRNFIVDGVSEGKSSYTYSKQQKRIKSISQEEADKKIKELGITADKFT
                     IIDPIVNKLNNIKQKADTYKDTQDYKADRELAYRNIEKLQPFYNKEWIVDQGNKVPSN
                     SKLLTTEVLSVTGMKDGQFVTDLSEIDKIMIHYADGTKEEKSVSPKPTSNVEQVKEYG
                     ITDLGDVVYTPNMVVKDRAQLLSDVKAKLDTITLESPEVRAITGNVGALYLEESFAEV
                     KGNLDKLVKALVENEDHQLNSDEAAIKAFLKKVEDNKAKIMMALTYLNRYYGIEYGDL
                     NIKNIMMFKPDFYGKIPSVIDRLIRIGSQEKNLKGDRTQNAYREIIAGDTGKGDLRSF
                     LDYNMRLFTNDTDLNDWFIHSAKNVYVVEPETTNPDFKNKRHRVFDGLNNNMHARMIL
                     PLLNLKKAHIFMISTYNTMAYSSFEKYGKNTEAEREAFKERINYVAKAQQTYLDFWSR
                     LALPNVRDRLLKSQNMVPTPVWDNQAYAGIKDANRRGYGTDGKVATPIRELFGPTDRW
                     HQINWNMGAMAKVYGKPYEDEQVFFMVTNMLEDFGISAFTHETTHINDRMAYLGGHGH
                     RPGTDLEAYAQGMLQTPDKSTSNGEYGALGINMAYHRQNDGNQWYNPDPDKLQSREQI
                     DHYMKNYNDALMMLDHLEADAVLPQLNGNTSRWFKKMDRQYRSGNSGHQFDKIRELTE
                     DEKKVAINTIDDLITNNLITKHGAPGDGTYNPTDFRSAYVNMNMMTGIYGGNTSTGAP
                     GAASFKHNTFRMWGYFGYENGFVGYASSKYQNAANKENNRMLGDDFIIKKVSNGRFTN
                     LEDWKKEYYKEVKAKGEKGFTAIEIDGKQITNYAELKVLFDKAVEADLAGGGTAKTVE
                     LKSKVFKALLKNSDGFTGNLFKA"
     gene            26716..28437
                     /gene="pabB"
                     /locus_tag="VW157_000583"
     CDS             26716..28437
                     /gene="pabB"
                     /locus_tag="VW157_000583"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9551931.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIHGCNAVRGLY
                     ELSLEEN"
     gene            28438..29055
                     /locus_tag="VW157_000584"
     CDS             28438..29055
                     /locus_tag="VW157_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9551932.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGATLTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            29105..29854
                     /locus_tag="VW157_000585"
     CDS             29105..29854
                     /locus_tag="VW157_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9551933.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQSVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTVAQTNTSSATTSGWVEQDGA
                     WYYFDENGNPVKNAWQGNYYLKSDGKMAQGEWIYDSSYQAWYYLTSDGSYAYSTWQGN
                     YYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTASSSNDSNSEYSAALGKAKSYNS
                     LFHMSKKRMYRQLTSDFDKFSNDAAQYAIDH"
     gene            29990..30949
                     /locus_tag="VW157_000586"
     CDS             29990..30949
                     /locus_tag="VW157_000586"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9551934.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFELAKEEDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            31046..31885
                     /locus_tag="VW157_000587"
     CDS             31046..31885
                     /locus_tag="VW157_000587"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9551935.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(31933..32103)
                     /locus_tag="VW157_000588"
     CDS             complement(31933..32103)
                     /locus_tag="VW157_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9551936.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            32224..33108
                     /gene="miaA"
                     /locus_tag="VW157_000589"
     CDS             32224..33108
                     /gene="miaA"
                     /locus_tag="VW157_000589"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9551937.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEGIHNRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            33101..34339
                     /gene="hflX"
                     /locus_tag="VW157_000590"
     CDS             33101..34339
                     /gene="hflX"
                     /locus_tag="VW157_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9551938.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            34332..34955
                     /locus_tag="VW157_000591"
     CDS             34332..34955
                     /locus_tag="VW157_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9551939.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            34970..35899
                     /locus_tag="VW157_000592"
     CDS             34970..35899
                     /locus_tag="VW157_000592"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9551940.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            35920..36675
                     /locus_tag="VW157_000593"
     CDS             35920..36675
                     /locus_tag="VW157_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9551941.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(36729..37637)
                     /locus_tag="VW157_000594"
     CDS             complement(36729..37637)
                     /locus_tag="VW157_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9551942.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            37701..37970
                     /locus_tag="VW157_000595"
     CDS             37701..37970
                     /locus_tag="VW157_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9551943.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            37970..38350
                     /locus_tag="VW157_000596"
     CDS             37970..38350
                     /locus_tag="VW157_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9551944.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            38439..38666
                     /locus_tag="VW157_000597"
     CDS             38439..38666
                     /locus_tag="VW157_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551945.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(38706..39431)
                     /locus_tag="VW157_000598"
     CDS             complement(38706..39431)
                     /locus_tag="VW157_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU9551946.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGDILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQTFQKGIVFLDGTIC
                     KPARLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            39593..41434
                     /gene="typA"
                     /locus_tag="VW157_000599"
     CDS             39593..41434
                     /gene="typA"
                     /locus_tag="VW157_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9551947.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            41454..41708
                     /locus_tag="VW157_000600"
     CDS             41454..41708
                     /locus_tag="VW157_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9551948.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKGKRR"
BASE COUNT        12877 a         7747 c         9405 g        11853 t
ORIGIN      
        1 gtatcccagc aatgagcgct ccagtatttg acaatattga agcagctatt aaagaacacg
       61 gattaagcta attctaattt gttaacattc atataacatc aaaaagggag gaactgttat
      121 ggatgcaatc tttgacctaa tcggaaaggt tttcaatccc atcttagaaa tgggtggacc
      181 tgtcatcatg ttaatcattt tgacagtatt ggctttactt tttggagtga aattctccaa
      241 agcgcttgaa ggtggtatca aacttgccat cgctcttaca ggtatcggtg ctatcatcgg
      301 tatgctaaat ggtgctttct cagcatcact agcaaaattc gttgaaaaca ctggtatcca
      361 attgagtatt accgacgtcg gttgggcacc acttgctaca atcacttggg gttctgcttg
      421 gacactatac ttcttgctca tcatgttgat tgtcaacata gtgatgctag ctatgaagaa
      481 aacagataca cttgacgttg atatctttga tatctggcac ttgtctatca caggtctctt
      541 gattaaatgg tatgctgaca acaatggtgt gagtcaaggg gtttcactct tgattgctac
      601 agcagctatc gtccttgtcg gtgtgttgaa aattatcaac tctgacttga tgaaacctac
      661 atttgatgac cttcttaacg ccccaagttc atcaccaatg acatcaactc acatgaacta
      721 catgatgaac ccagttatca tggttttgga taagattttt gaaaaattct tcccaggcct
      781 tgataaatat gactttgatg ctgctaaatt gaacaagaaa atcggtttct ggggatctaa
      841 attcttcatc ggtttcatcc ttggcatcgt tatcggtatt atgggaactc cacatccaat
      901 tgcaggtgtt gcagatgcag ataaatggcg tcttgttatc aaaggatggt tgtctcttgg
      961 tttgactgcc ggtgtatctt tggaactctt ctcacttatc ggttcatggt tcatcgcagc
     1021 cgtagaacca ctatcacaag gtattacaaa cgttgctact aaacgtcttc aaggacgtaa
     1081 attcaatatc ggtcttgact ggccattcat cgctggtcgt gctgaaatct gggcttgtgc
     1141 caacgtactt gcaccaatca tgttgattga agcagtgctt ctttcaaaag ttggaaatgg
     1201 tatcttgcca cttgcaggta tcatcgctat gggtgttact ccagctctct tggttgtaac
     1261 tcgtggtaaa ttgctccgta tgattatctt cggaacactc ttgttgccac tcttccttct
     1321 ttcaggtaca cttattgcac catttgcaac agaacttgct aaaggtgtag gtgccttccc
     1381 agaaggtgtg agccaaactc aattgattac tcactctact cttgaaggac caatcgaaaa
     1441 acttcttggt tggacaattg gtaacactac aactggtgat atcaaagcaa tccttggtgc
     1501 agtagccttc cttgtattct atatcggtat ctttgcttgg tacagaaaac aaatgatcaa
     1561 acgtaacgaa gagtacgcag caaaagcaaa ataatgcgct cctataaaat ataaactcaa
     1621 tgaaaatcaa agagcaaact aggaagctag ccgcaggttg ctcaaaacac agttttgagg
     1681 ttgtagataa gactgacgaa gtcagctcaa aacatggttt tgaggttgta gatgaaactg
     1741 acgaagtcag taatcatatc tacggcaagg cgacactgac gaagtttgaa ttggattttc
     1801 gcagagtgta aattgttgaa actaggttgt cataccatgt ataccacttg ggctttggta
     1861 gtagctaact gcactaaata taatataagg agggttttat ggggaaaggc cattggaatc
     1921 ggaaaagagt ttatagcatt cgtaagtttg ctgtgggagc ttgctcagta atgattggga
     1981 cttgtgcagt tctattagga ggaaatatag ctggagaatc tgtagtttat gcggatgaaa
     2041 cacttattac tcatactgct gagaaaccta aagaggaaaa aatgatagta gaagaaaagg
     2101 ctgataaagc tttggaaact aaaaatgtag ttgaaaggac agaacaaagt gaacctagtt
     2161 caactgaggc tattgcatct gagaagaaag aagatgaagc cgtaactcca aaagaggaaa
     2221 aagtgtctgc taaaccggaa gaaaaagctc caaggataga atcacaagct tcaagtcaag
     2281 aaaaaccgct caaggaagat gctaaagctg taacaaatga agaagtgaat caaatgattg
     2341 aagacaggaa agtggatttt aatcaaaatt ggtactttaa actcaatgca aattctaagg
     2401 aagccattaa acctgatgca gacgtatcta cgtggaaaaa attagattta ccgtatgact
     2461 ggagtatctt taacgatttc gatcatgaat ctcctgcaca aaatgaaggt ggacagctca
     2521 acggtgggga agcttggtat cgcaagactt tcaaactaga tgaaaaagac ctcaagaaaa
     2581 atgttcgcct tacttttgat ggcgtctaca tggattctca agtttatgtc aatggtcagt
     2641 tagtggggca ttatccaaat ggttataacc agttctcata cgatatcacc aaataccttc
     2701 acaaagatgg tcgtgagaat gtgattgctg tccatgcagt caacaaacag ccaagtagcc
     2761 gttggtattc aggaagtggt atctatcgtg atgtgacttt acaagtgaca gataaggtgc
     2821 atgttgagaa aaatgggaca actattttaa caccaaaact tgaagaacaa caacatggca
     2881 aggttgaaac tcatgtgacc agcaaaatcg tcaatacgga cgacaaagac catgaacttg
     2941 tagccgaata tcaaatcgtt gaacgaggtg gtcatgctgt aacaggctta gttcgtacag
     3001 cgagtcgtac cttaaaagca catgaatcaa caagcctaga tgcgatttta gaagttgaaa
     3061 gaccaaaact ctggaccgtt ttaaatgaca aacctgcctt gtacgaattg attacgcgtg
     3121 tttaccgtga cggtcaattg gttgatgcta agaaggattt gtttggttac cgttactatc
     3181 actggactcc aaatgaaggt ttctctttga atggtgaacg tattaaattc catggagtat
     3241 ccttgcacca cgaccatggg gcgcttggag cagaagaaaa ctataaagca gaatatcgcc
     3301 gtctcaaaca aatgaaggag atgggagtta actccatccg tacaacccac aaccctgcta
     3361 gtgagcaaac cttgcaaatc gcagcagaac taggtttact cgttcaggaa gaggcttttg
     3421 atacttggta tggtggcaag aaaccttatg actatggacg tttttttgaa aaagatgcca
     3481 ctcacccaga agctcgaaaa ggtgaaaaat ggtctgattt tgacctacgt accatggtcg
     3541 aaagaggcaa aaacaaccct gctatcttca tgtggtcaat tggtaatgaa ataggtgaag
     3601 ctaatggtga tgcccactct ttagcaactg ttaaacgttt ggtcaaggtt atcaaggatg
     3661 ttgataagac tcgctatgtt accatgggag cagataaatt ccgtttcggt aatggtagcg
     3721 gagggcatga gaaaattgct gatgaactcg atgctgttgg atttaactat tctgaagata
     3781 attacaaagc ccttagagct aagcatccaa aatggttgat ttacggttca gaaacatcat
     3841 cagcaacccg tacacgagga agttactatc gccctgaacg tgaattgaaa catagcaatg
     3901 gacctgagcg taattatgaa cagtcagatt atggaaatga tcgtgtgggt tgggggaaaa
     3961 cagcaaccgc ttcatggact tttgaccgtg acaacgctgg ctatgctgga cagtttatct
     4021 ggacaggtac ggactatatt ggtgaaccta caccatggca caaccaaaat caaactcctg
     4081 ttaagagctc ttactttggt atcgtagata cagccggcat tccaaaacat gacttctatc
     4141 tctaccaaag ccaatgggtt tctgttaaga agaaaccgat ggtacacctt cttcctcact
     4201 ggaactggga aaacaaagaa ttagcatcca aagtagctga ctcagaaggt aagattccag
     4261 ttcgtgctta ttcgaatgct tctagtgtag aattattctt gaatggaaaa tctcttggtc
     4321 ttaagacttt caataaaaaa caaaccagcg atgggcggac ttaccaagaa ggtgcaaatg
     4381 ctaatgaact ttatcttgaa tggaaagttg cctatcaacc gggtaccttg gaagcaattg
     4441 ctcgtgatga atctggcaag gaaattgctc gagataagat tacgactgct ggtaagccag
     4501 cggcagttcg tcttattaag gaagaccatg cgattgcagc agatggaaaa gacttgactt
     4561 acatctacta tgaaattgtt gacagccagg ggaatgtggt tccaactgct aataatctgg
     4621 ttcgcttcca attgcatggc caaggtcaac tggtcggtgt agataacgga gaacaagcca
     4681 gccgtgaacg ctataaggcg caagcagatg gttcttggat tcgtaaagca tttaatggta
     4741 aaggtgttgc cattgtcaaa tcaactgaac aagcagggaa attcaccctg actgcccact
     4801 ctgatctctt gaaatcgaac caagtcactg tctttactgg taaaaaagaa ggacaagaga
     4861 agactgtttt ggggacagaa gtagcaagag ttcgtacatt aattggtaaa gaaccaaaaa
     4921 tgccgaaaac agtaggtttt gtatacagcg atggtagtcg tgaaaaactt ccagtaactt
     4981 ggtctcaagt cgatgttagt caagcaggag ttgtgactgt taaaggtaca gcaaatggac
     5041 gtgaggtaga ggctcatgtc gaggttctag caattgcgaa agaattacca acagttaaac
     5101 gtattgctcc aaatactgac ttgaattctg tagacaaatc tgtttcttat gttttgactg
     5161 atggaagtgt acaagagtac gaagtagata gctgggagat tacggaagta gataaagcca
     5221 aactttcagt agctggatca cgtattcaaa tgactggtca gttagctgga gaaactattc
     5281 atgcaaccct tgtggtagaa gaaggaaatg ctgcagcacc tgtagtgcca actgttactg
     5341 ttggaggtga agcagtaaca ggtcttacta gtcgacaacc aatgcaatat cgtactctat
     5401 cttatggtgc ccaattgcca gaagtcacag caagtgctga aaatgctgat gtgacagttc
     5461 ttcaagcaag cgcagcaaac ggcatgcgtg cgagcatctt tattcagcct aaagatggtg
     5521 gccctcttca aacctatgca attcaattcc ttgaagaagc gccaaaaatt gctcacttga
     5581 gcttgcaagt ggaaaaagct gacagtctca aagaagacca aactgtcaaa ttgtcggttc
     5641 gagctcacta tcaagatgga acgcaagctg tattaccagc tgataaagta accttctcta
     5701 caagtggtga aggggaagtc gcaattcgta aaggaatgct tgagttgcat aagccaggag
     5761 cagtcactct gaacgctgaa tatgagggag ctaaaggcca agttgaactc actatccaag
     5821 ccaatactga gaagaagatt gcgcaatcca tccgtcctgt aaatgtagtg acagatttgc
     5881 atcaggaacc aagtcttcca gcaacagtaa cagttgagta tgacaaaggt ttccctaaaa
     5941 ctcataaagt cacttggcaa gctattccga aagaaaaact agactcctat caaacatttg
     6001 aagtactagg taaagttgaa ggaattgacc ttgaagcgcg tgcaaaagtc tctgtagaag
     6061 gtatcgtttc agttgaagaa gtcagtgtga caactccaat cgcagaagca ccacaattac
     6121 cagaaagtgt tcggacatat gattcaaatg gtcacgtttc atcagctaag gttgcatggg
     6181 atgcgattcg tccagagcaa tacgctaagg aaggtgtctt tacagttaat ggtcgcttag
     6241 aaggtactca attaacaact aaacttcatg ttcgcgtatc tgctcaaact gagcaaggtg
     6301 caaacatttc tgaccaatgg accggttcag aattgccact tgcctttgct tcagattcaa
     6361 atccaagcga cccagtttca aatgttaatg acaagctcat ttcctacaat aaccaaccag
     6421 ccaatcgttg gacaaactgg aatcgtagta atccagaagc ttcagtcggt gttctgtttg
     6481 gagattcagg tatcttgagc aaacgctccg ttgataatct aagtgtcgga ttccacgaag
     6541 accatggagt tggtgcaccg aagtcttatg tgattgagta ttatgttggt aagactgtcc
     6601 caacagctcc taaaaaccct agttttgttg gtaatgagga ccatgtcttt aatgattctg
     6661 ccaactggaa accagttact aatctaaaag cccctgctca actcaaggct ggagaaatga
     6721 accactttag ctttgataaa gttgaaacct atgctattcg tattcgcatg gttaaagcag
     6781 ataacaagcg tggaacgtct atcacagagg tacaaatctt tgcgaaacaa gttgcggcag
     6841 ccaaacaagg acaaacaaga atccaagttg acggcaaaga cttagcaaac ttcaaccctg
     6901 atttgacaga ctactacctt gagtctgtag atggaaaagt tccggcagtc acagcaaatg
     6961 ttagcaacaa tggtctcgct accgtcgttc caagcgttcg tgaaggtgag ccagttcgtg
     7021 tcatcgcgaa agctgaaaat ggcgacatct taggagaata ccgtctgcac ttcactaagg
     7081 ataagaactt actttctcat aaaccagttg ctgcggttaa acaagctcgc ttgctacaag
     7141 taggtcaagc acttgaattg ccgactaagg ttccagttta cttcacaggt aaagacggct
     7201 acgaaacaaa agatttgtca gttgaatggg aagaagttcc agcagaaaat ctgacaaaag
     7261 caggtcaatt taccgttcga ggccatgtcc ttggtagtga tcttgttgct gaggtcactg
     7321 tacgagtgac agacaaactt ggtgaggctc tttcagataa ccctaactat gatgaaaaca
     7381 gtaaccaggc ctttgcttca gcaaccaatg atattgacaa aaactctcat gaccgcgttg
     7441 actatctcaa tgacggagat cattcagaaa atcgtcgttg gacaaactgg tctccaacac
     7501 catcttctaa tccagaagta tcagcgggtg tgatcttccg tgaaaatggt aagattgtag
     7561 aacggactgt tgctcaagcc aaacttcact tctttgcaga tagtggtacg gatgcaccaa
     7621 ctaaactcgt tttagaacgc tatgtcggtc cagagtttga agtgccaacc tactattcaa
     7681 actaccaagc ctacgacgca gaccatccat tcaacaatcc agaaaattgg gaagctgtgc
     7741 cttatcgtgc ggataaagac attgaagccg gtgatgaaat taacgttacc tttaaagctg
     7801 tcaaagccaa agccatgaga tggcgtatgg agcgtaaagc agataagagc ggtgttgcga
     7861 tgattgagat gaccttcctt gcaccgagtg aattgcctca agaaagtacg caatcgaaga
     7921 ttcttgtaga tggaaaagaa cttgctgatt tcgctgaaaa tcgtcaagac taccaaatca
     7981 cctataaagg tcaacggcca aaagtctcag ttgaagaaaa taatcaagta gcttcaactg
     8041 tggtagatag tggagaagat agccttccag tacttgttcg cctcgtttca gaaagtggaa
     8101 aacaagtcaa ggaataccgt atccagttga ctaaggaaaa accagtttct gagaagacag
     8161 ttgctgctgt acaagaagat cttccaaaac tcgaatttgt tgaaaaagat ttggcataca
     8221 agacagttga gaaaaaagat tcaacactgt atctaggtga aactcgtgta gaacaagaag
     8281 gaaaaactgg taaagaacgt atctttacag cgattaatcc tgatggaagt aaggaagaaa
     8341 aactccgtga agtggtagaa gctccgacag accgcatcgt cttggttgga accaaaccag
     8401 tagctcaaga agctaaaaaa ccacaagtgt cagaaaaagc agatacaaaa ccaattgatt
     8461 caagtgaagc tagtcaaact aataaagccc agttaccaaa tacaggtagt gcggcaagcc
     8521 aagcagcagt agcagcaggt ttagctcttc taggtttgag tgcaggatta gtagttacta
     8581 aaggtaaaaa agaagactag aaattttgat accttcttta tcattaaaga ccaaagaaaa
     8641 actcttgtcc taaatagaag ataaagagtc tgggacaaaa tagttctcaa ccacaaaaaa
     8701 gctagatatt tccaattgtg gaactctagc tttttaattt tgagtcgttc tcttattgta
     8761 tagtatattg aaactagaat agtacacttc tacttctaaa acattgttag aaatcgattt
     8821 gactgtcctt atcgatttgt cctgttctta tttcatttca ctatatttta ggaggttatt
     8881 ggccataagt accaatccca tgtcaattct cacttgacgc ttgcccctca gattacatct
     8941 cttgtaaccc aaagaagcct ttatctgccc aaagacaggt tccacatcaa tcttgcgttg
     9001 agcgaaaatc tgtctacctt ggggagataa aagcgcctga cattctttag ctttcaagtt
     9061 ttgatagcgt tcgttcatat atagtcccct tttgaggggc tgatttaggt tcgtcggcgt
     9121 agtaaatctt gatttcctgt tgaaagtctg tctgtgtttt ctgatgtttg gtatggtgaa
     9181 aacgataata ccagccatca ggatgtgtgt agctatcctc cttgtcatta tagtgccaat
     9241 tcgctaagtt tttagctgac tctttatacc ctctcttttg ttccttatca aacatggcat
     9301 atttaatcag gaggggagtg atttcttctt gaaaatagac ttgtatctgt tcttgaagtt
     9361 tggcggagaa tttctctgtc gttttcttcc acacaaaact atacttgttg gcattggctt
     9421 caatcttagt tccgtcaata aacaaacaat ctaaggtcac taactcttcc atttttcaat
     9481 ttttcgacca gagaaaatcc cttgtgaata ggcaaataga agagtagata caagcatttt
     9541 agggtgataa gacgggcgat caaaggcatg atagaaggcg tggaaatgac aatcctccaa
     9601 ggtattcacc actttttcaa tagtaaaaac gagatggtct tgtggcaaga aggaactgat
     9661 ttctagtggt aaagttgttt gatttgtgtt atagtgaata tgcatgggat agccttttta
     9721 aatggtttat tgggcaattc tattttacca agactatcgt aaccatgcaa aaaaagcaac
     9781 ggcaaattcg ttgctttttt tggagataag agactattgt cccagactct tttctttgta
     9841 taaaattgta tagtgttatt gtctatacta ttttgtagag aaagcaaaaa tccctagaaa
     9901 atgcttaggg attcgactat ttcattaaga acaattcaac aatcatctct atatacatga
     9961 caagggttaa taagaatcct gtacgccaga agaatttgat gaatttagga tagtaaaagc
    10021 tgcgtttttt caaaaggaag aaaaagacga gaataatggc taggatcgag agggctagcc
    10081 ccagtctagg gaggaaatta tgggtaaagg ttttagctgt aatcaggtaa tattcaaata
    10141 ccaagaccgg aaaagccaaa tccgcaaagt ttcgtcctag tttttttaat ctaaaaagtt
    10201 tggttaggat aatgcagacg actaaggtca gtatcaataa taaaatagat gctaatttca
    10261 ttaaaatcat acccatattg tatcataaaa aaaggctaat ggaaacgaga aacagaggaa
    10321 tttataggaa agtcaggctt ttgagattgt gggtgatttt tgttataatg aaagttataa
    10381 aatcttataa taccttgaat gattattatc gaaaactctt tggagaaaag acttttaaag
    10441 tccctattga tgcgggattt gactgtccca atcgtgatgg aactgtggct catggaggct
    10501 gtactttttg tacggtttca ggttctggag atactattgt agccccagat gcgcctatcc
    10561 gtgagcaatt ttataaggaa attggcttta tgcaccgtaa gtggccggat gttcagaagt
    10621 atctggttta ttttcaaaat tttaccaaca cccatgaaaa ggtggaagtt attcgagagc
    10681 gttatgaaca ggctatcaat gagccaggtg tggtaggaat caatattgga acgcgcccag
    10741 actgtttacc agacgaaacc atcgaatatt tggctgagtt atcggagtgc atgcatgtga
    10801 cggttgaatt gggcttgcaa actacttatg aagcaacctc tgacctgatt aaccgtgcgc
    10861 attcttatga attgtaggtg gaaacggtca agcgtttgag aaagtatcca aaaattgaga
    10921 ttgtttccca tttgatcaat ggtttgcctg gtgaaaccca tgagatgatg gttgaaaatg
    10981 tccgccgttg tgtcacggat aacgatattc aagggattaa actgcatttg ctccatctta
    11041 tgacaaatac tcgtatgcaa cgagattacc atgagggacg tttacaattg atgagtcagg
    11101 acgaatatgt cagggtcatc tgtgaccaac tggaaattat tcccaaacat atcgtcatcc
    11161 atagaattac aggagatgcg cctagggata tgctgcttgg gcctatgtgg aacctcaata
    11221 aatgggaagc tctcaacagc attgagatgg agatgcgacg tcgtggaagt gttcaaggat
    11281 gcaaggctgt aaaacaggag tttgaaaatg aaaagaccac ttgagatggc acatgatttt
    11341 ttggctgagg tcgtgacaaa agaggatgtc gtagtggatg cgactatggg aaatggtcat
    11401 gacacgcttt ttttagccaa gctagccaag caagtctatg cctttgatat tcagaagcaa
    11461 gccttggaaa agacccaaga gcgtttgcat caggctggct tgacaaatgc ccagttaatc
    11521 ttgcaaggcc atgagacact ggaccagttt gtgacaaaag ctaaggcagg gatttttaat
    11581 ttgggctatt tgccgtcagc tgataagtct gtcatcaccc gaccgcagac aacgattgag
    11641 gcattagaaa agctatgtgg cttacttgtc aaaggtggac gaattgctat tatgatttac
    11701 tatggtcatg aaggaggcga cctcgagaga gatgctgtct tggattttgt cagccagttg
    11761 aaccaacaag agtacacagc tgccatttac cgaactttaa accaagtaaa caacccacca
    11821 tttttagtaa tgattgaaaa attagaaaga tatagacatg gataaacaat acctacgtga
    11881 aaagctggat gccatgcgcc agaattttgt tgaatcaacc caccacgaac gagcggtggg
    11941 tgtgttagac caagcacata tgagcaaaaa aatgcttaaa atcaagaaaa aattagttgc
    12001 tcttgaaatg gaacggtgcc agagaaaaat tgagcacaaa gactgttcca agattgacca
    12061 aaaaatcaaa gagcagaagg agatttttga atcttgttgt aaaaaagatt aaggaggtcg
    12121 tgcgtggaat tactgattta ccttatccta tttttactgg tcttgattgt ctcgagtaca
    12181 accaataagc tcctgccttt tttgccactc cctttggtgc agattctttt gggaattgtg
    12241 attggtctat ttttacccaa taccgacttt cacctcaata cagagttgtt tttggcactg
    12301 gttattggac ccttgctttt tcgagagtcg gaagaagcgg atattacggc tattttaaaa
    12361 cactggcgaa tcattgttta tctcatattt ccagtgattt ttatctcgac cctgagtttg
    12421 ggaggtttgg cccatcttct ttggctcagc cttcccttgg cagcttgctt ggctgttggg
    12481 gcagctcttg ggcctacgga tttggtggcc tttgcctctc tttcagagcg gtttagcttt
    12541 cctaagcgcg tgtccaatat ccttaagggt gaaggacttt tgaatgatgc atctggtttg
    12601 gtggcttttc aggtagcttt gacagcttgg acaactggag ccttttccct tgggcaagcg
    12661 agtagttcgc tcatcttttc aatcctaggc ggttttttga ttggcttttt aacagccatg
    12721 accaatcgtt tcctccatag cttcttgcta agtgtgcgag caacggatat tgccagcgaa
    12781 cttttattag aattgagttt gcctctggtg accttcttcc tggcagaaga agtccatgtt
    12841 tcagggatta ttgccgttgt agttgctggg attttaaagg caagccgttt taagaaaatt
    12901 acgctcctcg aagcccaagt ggatacggtg accgagacgg tctggcacac agtcaacttt
    12961 atgctcaacg gttctgtctt tgtgatttta gggatggagc tggaaatgat agcagagcct
    13021 atcttaacca atccaatcta taatccctta ctgttattgg gatctcttgt tgcccttacc
    13081 tttgtcctct ttgccattcg ttttgtcatg atttatggct attatgccta taggacccga
    13141 cgtctcaaga aaaagctaaa taagtatatg aaggacatgc ttctcttgac cttctcaggt
    13201 gttaagggaa ccgtgtcgat tgctacaatt cttctgatac caagtaattt agagcaggaa
    13261 tatcctctct tacttttcct tgtcgcaggt gtgacgcttg taagcttttt aacaggtctc
    13321 ttggttctgc ctcatctttc tgatgaacag gaagaaagca aggactatct catgcatatt
    13381 gccattttga atgaagtaac gctagagttg gaaaaagagt tggaaggcac tagaaataaa
    13441 cttcctctct atgcggctat tgacaattac catggacgta ttgaaaatct cattttaagc
    13501 caagaaaacc aggatgatca agaagactgg gctgctttga aactcttgat tcttagtatt
    13561 gaaagtgatg gattggaaca ggcctatgaa gaggggaaca ttagcaatcg tgcttaccga
    13621 gtttaccaac gttatctgaa aaatatagaa cgaggcatca atcgtaagtt tgcgtcacga
    13681 ttgacctatt atttccttgt ttctttgcgg attttacgtt ttctccttca tgaagttttt
    13741 actctcggaa agaccttccg tagctggaag gacaaggagc aaagccgtct ccgtgctctt
    13801 gattatgacc aaattgcaga gctctatctt gccaatacag agatgattat tgaaagtttg
    13861 gaaaacctga agggagtcta tagacgctct ttgattagtt ttatgcagga gtctcgtctt
    13921 cgtgaaacag ctattatcag cagtggtgcc tttgtcgaac gggttattac acgtatcaaa
    13981 cctaacaata tcgatgaaat gctgaggggc tattatttgg agcgcaagtt gatttttgaa
    14041 tatgaagaga aacgattgct tacgaccaag tatgccaaga aattacgaca aaatgtcaat
    14101 aacttagaga actattctct gaaggaagct gccaataccc tgccttatga tatggtggaa
    14161 ttggtaagaa gaaattagtt aatactcttc gaaaatctct tcaaaccgcg tcagcgtcgc
    14221 cttggattat atatgtgact gacttcgtca gtttcatcta caacctcaaa gcagggcttt
    14281 gagcaacctg cggctagctt cctagtttgc tatttgattt tcgttgagta taagattata
    14341 agtgaaggag tgtgacatga aaaagtggtg gaaagagctg atagacaagc ctctattaaa
    14401 agcttttttg cattattatc aagcatcaga tagtgagttg accagtgtcg cagtagccta
    14461 ctattggttg atttcgattt ttcccctgct tttggtggtg gtcaatatac tcccctattt
    14521 tcagattcct gtgggagaat ttctgggctt tgtgaaagac gtcctgcccc caagcctcta
    14581 tgaaggtgtg gaaaaaatag ccagagaagt cttgacgcaa ccttcaacag gtttattgag
    14641 tttttctgtc ttgtcggcgc tatggagttt ttcaaaatct atgaatttct tgcaaaaagc
    14701 ttttaacaag gcttatggcg tcgaaaagag tcgtggactt atttcccatc agatggtgag
    14761 tctcctagtc agttttggct tgcagctcct ctttgcgttt gccttgtttt tgagcttatt
    14821 tgggcagatg attttggatt tacttgctca ttattggaca aaagatggca ttatctatca
    14881 ggctttacaa gggttggcag gtcctctgac ttacgccctg ctctttgcta tcttggtcat
    14941 gctttattat tttcttccca atctatctaa caggaaaatt agctataccc taccaggcag
    15001 tgcctttgtt ctcttagtga ttttagttct actaactctg ttctctagtt atctcaatta
    15061 ctatgtccat catttagtgg atgtccgaat tttaggttcc gttcttcttg ttgttatgat
    15121 gttttggttt atcttgattg ctaaaattgt tatcctaggc gcggtgatca atgccagtgt
    15181 gcagagtttg aaagatccga gctttaataa agattaacta tttatctatt acaaaagcgc
    15241 atagtataaa ggttctataa agccttgata ctatgcgctt ttgtgctttg acagtttttt
    15301 gtatagtttt tgtccaagat ttctaaatca tctgattaaa ttcctatttt tccctatcat
    15361 tgtccctgtt ttttcgaaaa tggaaggagt ataataaacc tatcaataaa atgataggag
    15421 gaactttatg ggtcatattt tcttttttct aagtgtcttt ttggcaggga ttctatcctt
    15481 cttttctcct tgtatcttac ccttgttacc agtctatgca ggggtcttgt tggatgataa
    15541 ggatggtgct caggcttcta gtggcaaatt ttccatctca gttgctagtt tattacgaac
    15601 tctggccttt atagcaggaa tttcctttat ctttattttg ttgggctatg gagctggttt
    15661 tttaggcgat ttgctttatg cttcttggtt ccaatatgtg acgggtgcgg ttatcattct
    15721 cttaggcttg caccagatgg agattctaca ctttaaggga ctttataagg aaaagaggtt
    15781 acaactgcaa ggacaggggc aacatggtaa gggctatagt caggcatttt tactgggttt
    15841 gacctttagt tttgcttgga cgccttgtgt ggggccggtt cttggctctg ttttagcctt
    15901 ggcggcttca ggtggctcag gtgcttggca gggagcgggt ctcatgttgg tgtacacgtt
    15961 gggcttggcg ctaccattct tgcttctagc tctagcctct agttatgttt tgaaacattt
    16021 ccgaaaactt catccctatc tcggaatcct caaaaaagtg ggtggttttc tcattattgt
    16081 gatgggactc ttggttctat ttggaaatgc ttcaatttta agtcaattat ttgaataaaa
    16141 atggaaagga atatcagtat gaaaaaatgg caaacatgtg ttcttggagc aggttcgctc
    16201 ctttgtttga cggcttgttc aggcaagtcc atgaccagtg aacaccaaat gaaagatgag
    16261 atgaagatgg agcagacagc tagtaaaata agcgcagcta aagggaaaga agttgcagat
    16321 tttgaactga cgggagtaga tggcaagacc taccgcttat ctgattacaa gggcaagaaa
    16381 gtctatctca aattctgggc ttcttggtgt tccatctgtc tggctagtct tccagacacg
    16441 gatgaaatcg ctaaagatgc tggtgacgat tatgtggtat taacagtagt gtcaccagga
    16501 cataagggag agcaatctga agaagacttt aagaactggt ataagggatt ggattataaa
    16561 aatttcccag tcctagttga cccatcaggt aaacttttgg aaacttatgg cgttcgctct
    16621 tacccaactc aagcctttat agatggtgaa ggaaaattgg tcaaaacgca accaggtttt
    16681 atggaaaaag atgcgatttt gcaaactttg aaggaattag cctaggaggt gtcctatgaa
    16741 tgataagcta aaaatcatct tgttgatagg agtatttttc ctagccataa ccggtttcta
    16801 ttttctattg atgcgaaatg cagggcagac agatgcctcg caaattgaga aagcggcagt
    16861 cagccaagga ggaaaaacag tgaaaaaaac agaaattagt aaagacgcag acttgcacga
    16921 aatttatcta gctggaggtt gtttctgggg agtggaggaa tacttctccc gcgttccagg
    16981 agtgacagat gccgtatcag gctatgcaaa cggtagaggg gaaacaacca agtacgaatt
    17041 gatcaaccaa acaggtcatg cggagactgt tcatgttacc tatgatgcta atcaaatttc
    17101 tctcaaggaa atcctgcttc actatttccg cattatcaat ccaaccagca aaaataaaca
    17161 aggaaatgat gtgggaaccc agtaccgtac cggtgtatat tacacagatg acaaggattt
    17221 ggaggtaatc aaccaggtct ttgatgaagt tgctaagaaa tacgatcaac ctctagcagt
    17281 tgaaaaggag agtctgcaga atttcgtagt ggcagaggat taccaccaag actatctcaa
    17341 gaaaaatcca aatggctact gccatatcaa tgtcaatcag gcagcctatc ccgtcattga
    17401 tgccagcaaa tatcccaaac caagtgatga agaattgaaa aagaccctgt cacctgagga
    17461 gtatgcagtc acccagaaaa atcaaacaga acgagctttt tcaaaccgct attgggataa
    17521 atttgaatct ggtatctatg tggatgtagc aactggcgaa cccctctttt catcaaagga
    17581 caagtttgag tctggttgtg gttggcctag ttttacgcaa cccatcagtc cagatgttgc
    17641 cacttataag gaagataagt cctacaatat gacgcgcatg gaagtgagaa gccgagttgg
    17701 agattctcac cttgggcatg tctttacgga cggtccacag gacaaaggtg gcttgcgcta
    17761 ctgtatcaat agtctttcta tccgctttat tcccaaagac caaatggaag aaaaaggcta
    17821 tgcttattta ctagattatg ttgattaaga agtctttcct aagcagttag aggagaattt
    17881 tgctatactg atactagtaa gtgacaaagg agagaagcat gacctataca atcttaatcg
    17941 tagaagatga atatctggtg agacaaggct tgaccaagct ggtcaatgta gcagcctacg
    18001 atatggaaat catcggtcag gctgaaaatg gaagacaggc ttgggaattg atacaaaagc
    18061 aggtgccaga cattatttta accgatatca acatgcctca gctgaatggc atccagttgg
    18121 ccagtctggt ccgagaaacc tatcctcagg tgcatctagt ttttttaaca ggctacgatg
    18181 attttgatta tgccttgtct gctgtcaaac taggtgtgga cgattacctg ctcaagccct
    18241 tttctcgtca ggatatcgag gaaatgctgg ggaaaatcaa gcaaaagttg gacaaggaag
    18301 agaaagaaga gcggttacaa gatttattga ccgataagtt tgaaggaaac atggcccaga
    18361 aaatccagtc ccatatggct gatagccagt ttagtttaaa ggctttggcc agtgacttgg
    18421 gctttagtcc gacttatctg agttctttga ttaagaaaga gctgggtctg ccttttcagg
    18481 attatctggt gagagaacgt gttaaacaag ccaaactctt gcttttaact acagatctga
    18541 agatttatga gattgcagaa aaggttggtt ttgaagatat gaactatttt acccaacgtt
    18601 ttaaacagat tgcgggtgtg acacctcgtc agtttaagaa gggagaaggc cgatgaagcg
    18661 ttcttctctc ctagtcagaa tggttatttc catctttctg gtctttctca ttctcctagc
    18721 tgttgttggg actttctact atcaatctag ttcctctgcc attgagacta gcattgaggg
    18781 caatagccag acgaccatca gccagactag ccactttatc cagtcttata tcaaaaaatt
    18841 agaaaccacc tcgaccagtt tgacccagca gacggatatt ctagcctatg ctgagaatcc
    18901 tagtcaagac aaggtcaagg gaattcgaga tctgtttttg accatcttaa aggcagacca
    18961 ggacttgaaa acggtagttc tggtgaccaa atctggtcag gtcatttcta cagatgacag
    19021 tgtgcagatg aaaacttcct ccgatatgat ggctgaggat tggtaccaaa aggccattca
    19081 tcagggagcc atgcctgttt tgactccagc tcgtaaatca gatagtcagt gggttatttc
    19141 tgtcactcaa gaacttcttg atgcaaaggg agccaatctg ggtgtgcttc gcttggatat
    19201 ttcttatgaa actctggaag cctatctcaa tcaacttcag ttggggcagc agggttttgc
    19261 ctttatcatc aatgaaaacc atgaatttgt ttaccatcct caacacacag tttatggttc
    19321 atctagcgaa atggaggcta tgaaacccta catcgagacg ggtcagggct atactcctag
    19381 ccacaaatcc tacgtcagtc aggaaaagat tgcaggaact gattggacgg tacttggcgt
    19441 gtcttcgttg gagaagttag acaaggttag gagtcaactc atgtggacct tgcttggggc
    19501 tagtgttaca tctcttcttg cctgtctctg cttggtgtgg tttagtctta aacgctggat
    19561 tgctcctttg aaggatttga gagaaaccat gctggaaatt gcttctggtg gtcagaatct
    19621 tcgtgccaag gaaacgggtg cttatgaact gagagaggtg actcgccaat ttaatgccat
    19681 gttggatcag gttgatcagc tgatggcaga tgttcgcagg caggaagaaa cgacccgtca
    19741 gtaccaactt caagccctat caagccagat taatccgcat ttcctctata acaccttgga
    19801 caccatcatc tggatggctg aatttcatga tagtcagcga gtggtgcagg tgaccaagtc
    19861 cttggcaacc tatttccgct tggcgctcaa tcaaggcaag gacttgattt gtctctctga
    19921 cgaaatcaat catgtccgtc agtatctctt tatccagaaa caacgctatg gagataagct
    19981 ggaatacgaa attaatgaaa atgttgcctt tgataattta gtcttaccca agctggtcct
    20041 acaacccctt gtagaaaatg ctctttacca tggcattaag gaaaaggaag gtcagggcca
    20101 tattaaactt tctgtccaga aacaggattc gggattggtc atccgtattg aggatgatgg
    20161 cgttggcttc caagatgctg gtgatagtag tcaaagtcaa ctcaaacgtg ggggagttgg
    20221 tcttcaaaat gtcgatcaac ggctcaaact tcattttgga gccaattacc agatgaagat
    20281 tgattctaga ccccaaaaag ggacgaaagt tgaaatatat ataaatagaa tagaaactag
    20341 ctaactccca gtcaattctg ggagttttat gcgtaattgg gcaaactttc taggttgtct
    20401 atcttgctaa aaacaagaaa aaacggtatg atagaagagt gacaacagag gtaacagaga
    20461 tgaacgagac acagattcaa agagaaacac gtcaggtagt ggaagatgtt ttagaaaaga
    20521 ccaatttgaa gcagggggcc ctttttgttt taggtctatc ttctagtgag gtgctaggtg
    20581 gtcagattgg gaaagaatcc agtcaagaga ttggtgagct gatagtggag actatcttgg
    20641 gtattctagg tagtagaggt atccatttag cagttcaagg ttgtgagcat gtcaatcggg
    20701 ctctcgttgt tgaacgtcag gtggcagagc agtttgatct ggaaattgtc agtgtccatc
    20761 ctactcttca tgcaggaggt tcggggcagt tggcagcctt taagtttatg caggatccag
    20821 ttgaggttga atttatcaag gctcatgctg gattggatat cggagacact gcaattggca
    20881 tgcatgtcaa gcatgttcag gttccgattc gccctatttt gagagagatt ggtcatgccc
    20941 atgtaacggc actgactagt cgtccaaaat taatcggagg tgcgcgtgcg cactatccgc
    21001 aagatgctat taggaagttt tgagaatgaa taaaggagat aaaagtgttt aaaaaagacc
    21061 gtttttcaat tcgtaagatt aagggagttg taggctctgt atttcttgga agccttttga
    21121 tggctccttc tgtagtggac gcagccacct atcactatgt aaataaagag attatttcac
    21181 aagaagctaa agatttaatt cagacaggaa agcctgacag gaatgaagtt gtatatggtt
    21241 tggtgtatca aaaagatcag ttgcctcaaa cagggacaga agcatctgtt ttgacagctt
    21301 ttggtttgct gactgttggg agcttgcttt taatctacaa gagaaagaaa attgctagcg
    21361 tctttctagt tggagctatg ggattggtag ttcttcctag tgcaggggct gtagacccag
    21421 ttgcgaccct agcgctggct agtcgagagg gtgttgttga aatggagggc tatcgctatg
    21481 ttggttatct atcaggtgac atcctcaaaa cgcttggctt ggacactgtt ttagaagaag
    21541 cctcagctaa acttggagag gtaactgtag tcgaagctga gaatccccaa gtaacaataa
    21601 atcaggagca agctaaggca gaaaaccaag cagtagagac agaggaagca cctaaaacag
    21661 aagaaagtcc aaaggaagaa ccaaaatcgg agataaaacc tactgacgac acccttccta
    21721 aagtagaaga gggaaaagaa gcttcagcag aaccagctac agttgaagaa gtaggtggag
    21781 aagttgagtc aaaaccagag gaaaaagtag cagttaagcc agaaagtcaa ccatcagaca
    21841 aaccagctga ggaatcaaaa gttgaacaag caggtgaacc agtcgcgcca agagaagacg
    21901 aaaaggcacc agtcgagcca gaaaagcaac cagaagctcc tgaagaagag aaggctgtag
    21961 aggaaacacc gaaacaagaa gagtcaactc cagataccaa ggctgaagaa actgtagaac
    22021 caaaagagga gactaaaaca gctaaaggta cgcaagaaga aggcaaagaa ggtcaagcac
    22081 cagtacaaga agtaaaccca gaatataaag taacaacagg tacagttgag aagtctaccg
    22141 aaagtgaatt agatttcaca atagaagtag ttccggatga cacaaaatat gtagatgaag
    22201 aagttgttga acgtcaaggg tctaaaggag ttcaaattac gaaaacaact tacgaaacag
    22261 tggaaggtgt gaaaacagac aaagtccttt ctacaactac agaagtaaaa actcctgctg
    22321 ttcctaaagt cgttaaaaaa ggaacaaaac cagttgaagg aacaacagtt gaaacaagag
    22381 aagaagtcat tccttttgaa acaaaagaac aagaagatga cactttaaaa cgcggaacaa
    22441 gacaagtagt tcaaaaaggt gtagaaggta agaaacaaat tactgaaact tataaaacaa
    22501 ttcgtggaga aaaaacaaat gaagctccaa tagttaagga aacagtaatt gaacaacctc
    22561 aagatgagat tatcaaaaaa ggcacaaaag gtttagaaaa accaacctta gaatgggtta
    22621 aaacagataa agatgtcttg aagaagagtg cgacagccag ttatacattg aataaaccag
    22681 atggggtaga aatcaaatcc atcaaagtag ctctaaaaga taatactgga acagttatta
    22741 aagaagtaac ggtagaagaa aataacttaa acgctacttt agacaacttg aaatattatc
    22801 aaggttatac actatccaca accatggtct ataatcgagg cgaaggcgaa gaaactgaaa
    22861 tgttagaaga taaagaagtt cagttagacc ttaaaaaagt tgaaatcaaa aacatcaaag
    22921 aaactagctt gatgagtgtg gatgatgctg gtgttgaaac agataagagc ttacttactg
    22981 aaaaaccgac agacgttgct ccactttacc tacgagtgac aactcatgat aacaaaacaa
    23041 caagactggc agtaagttca gttgaagaag ttgttgttga tggaaaaaca ttgtacaaag
    23101 tggtagctaa agctcctgat ttagttcaac gtagagaaga tgatacacta agcgaagagt
    23161 atgtacatta ctttgaaaaa caattaccaa aagtagataa tgtgtactat agctttaacg
    23221 agttaattac agatatgcaa aaaaatccta ctggtacatt caaactaggt gcagatctta
    23281 atgcagcgaa tactccaact ccaagtaaat catatgtaac aggtgaattt aaaggtaaat
    23341 tatcaagtgt tgatggtcaa cattatacga ttcacaatac tgcgcgccca ttatttaaca
    23401 atattgttgg tggtaccgtt aaaaatatta atcttaacaa tgtaaatatc gatatgcctt
    23461 gggctgatca aattgcatct gtagcaaatg tgatcaaagg tggtagcctt attgagaatg
    23521 ttaaagtcaa aggtaatgtg ctcggcagaa actgggtatc tggtttcatt gataaaattg
    23581 acagtggtgg tagattagtt aatgttgcat ttattggtaa tgtaacttct gttggagatg
    23641 gggataagtt cttaacaggt attgttggag aaaactggaa aggatatgtc gaaaaagcat
    23701 acgttgaagc taatattaaa ggtaagcgag ctaaagctgc tggtatatct tattggtcgc
    23761 aaaatagcgg agataataat gctgtcggta gagatggagc aattaagaaa tctgtcgtta
    23821 aaggttcaat agatgtagaa aaaccaattg aagttggtgg cgctgttgga agtttaaatt
    23881 atcttggtta tatagaagac actgttgcta tgatgaaagt taagaacggt gaaattttct
    23941 acggttcgca tgatatcgat accgatccat attacacagg agaacgagta aatagaaatt
    24001 tcattgttga cggtgtaagt gaaggtaaat cttcgtatac atactctaaa caacaaaagc
    24061 gtattaaatc aatatctcaa gaggaagcag ataagaaaat taaagaattg ggtatcacag
    24121 cagataaatt tacaattatc gatccaattg taaataaact taacaacatc aaacaaaaag
    24181 ctgatactta taaagatact caagattaca aagcagaccg tgaacttgct tatcgtaata
    24241 tcgaaaaact tcaaccattc tacaataaag agtggattgt agaccaaggt aataaagttc
    24301 catcaaattc taaattatta actacagaag tattatctgt tacaggtatg aaagatggtc
    24361 aatttgtgac tgacttatca gaaattgata aaattatgat tcactatgcg gatggtacaa
    24421 aagaagaaaa atctgtatca cctaagccta catcaaacgt agaacaagta aaagaatatg
    24481 gtattacaga tttaggagat gtcgtttaca caccaaatat ggtggttaaa gaccgtgctc
    24541 aattactaag tgatgttaaa gcgaaattag atacgattac tcttgaatct cctgaagttc
    24601 gtgcgataac aggtaatgtt ggagcattat acttagaaga aagttttgca gaagtaaaag
    24661 gaaatcttga taaattagta aaagctcttg ttgaaaatga agatcatcag ttaaatagtg
    24721 atgaggcagc catcaaagca tttcttaaga aagtagaaga taataaagct aaaatcatga
    24781 tggcacttac ttacctcaat cgttattatg gtattgaata tggtgactta aacattaaga
    24841 atataatgat gtttaaacca gatttctatg gcaagatacc aagtgttatt gatagattaa
    24901 ttcgaattgg ttcgcaagaa aagaacttga agggtgatag aacccaaaat gcatatcgtg
    24961 aaattattgc aggagatact ggtaaaggtg atttacgtag tttcttagat tataatatgc
    25021 gtctcttcac aaatgataca gatcttaatg actggtttat ccattcagcg aaaaatgttt
    25081 atgttgtaga accagaaaca acaaatccag atttcaaaaa taaacgtcat cgtgtttttg
    25141 atggtctgaa taataatatg catgcacgta tgattttacc attattgaac ttgaaaaaag
    25201 cacatatctt tatgatttca acctataata ctatggctta tagttcattt gaaaaatatg
    25261 gtaaaaatac ggaagctgaa cgtgaagcgt tcaaggaacg tatcaattat gtagctaaag
    25321 ctcaacaaac atacttagac ttctggtcac gtcttgcact tccaaatgtt cgtgatagac
    25381 ttcttaagag tcaaaatatg gttcctacac ctgtatggga taatcaagct tatgcgggta
    25441 ttaaggatgc taatcgtcgt ggatatggta cagatggtaa ggtagcgaca ccaattcgtg
    25501 agttatttgg tccaacagac agatggcatc aaattaattg gaatatggga gctatggcta
    25561 aagtttatgg aaaaccatat gaagatgaac aagttttctt catggtaaca aatatgttag
    25621 aggactttgg tatttcagcc tttacacatg aaacaactca cataaatgac cgtatggcat
    25681 atcttggtgg tcatggacat cgtccaggta cagaccttga agcatatgct caaggtatgt
    25741 tacaaacacc tgataaatca acatcaaatg gtgagtatgg agcattaggt attaatatgg
    25801 cttatcatcg tcaaaatgac ggaaatcagt ggtataaccc agatcctgat aaattacaaa
    25861 gtcgtgaaca aattgatcac tatatgaaga actacaatga tgctcttatg atgttagatc
    25921 atcttgaggc tgatgctgta ttaccgcagt taaatggaaa tacgagtcgc tggttcaaga
    25981 aaatggatag acaataccgt agtggaaatt caggtcatca gttcgataaa attcgtgagt
    26041 tgacagagga tgagaaaaaa gtagcaatca atacaattga tgatttaatt actaataacc
    26101 taataacgaa acatggtgct cctggtgatg gtacttataa tccaacggac tttaggtctg
    26161 cttatgttaa tatgaacatg atgactggta tttatggtgg aaatacaagt acaggtgcac
    26221 cgggtgcagc atcatttaaa cataatacat tccgtatgtg gggatacttt ggatatgaga
    26281 atggatttgt tggatatgct tctagcaaat atcaaaatgc agccaataaa gaaaataata
    26341 ggatgttagg tgatgacttc attattaaga aagtttcaaa tggaagattc actaaccttg
    26401 aagattggaa gaaagagtac tataaagaag tgaaggcaaa aggtgaaaaa ggatttactg
    26461 caattgagat tgatggtaaa caaattacca attatgctga actaaaagtc ttatttgata
    26521 aggcggttga ggctgatcta gcaggtggag gaacagctaa aactgtagaa cttaaatcga
    26581 aagtctttaa agcacttctt aagaattcag acggattcac aggaaatctt tttaaagcat
    26641 agattgtatt aaatagatga gtaaaaaggt agagcagctt tgtctgtatc tgccttttta
    26701 tgctatactt aaagtatgca tagaaaaaca gtgattgatt ttaggacttt gggggagaga
    26761 tataccttta cccagcctat taaagagttg aaaacgagaa atgtagaaga agtggcagat
    26821 ttgctggcac aagtggaaag ctaccaagag caaggttatt atgtggtggg gtatgtcagc
    26881 tacgaggctg cacctgcttt tgaggaaaaa ttagcagttc acaaggatcc cctactggga
    26941 gagtacctgc tttattttac tgttcatgat agcgtagaaa cttcccctat tcctctgact
    27001 tatgaggata ttgatttgcc ctcaaattgg caggaagtaa cgtctgcagc agactatgaa
    27061 aaggccattg cccaaattca ccatcattta cggcagggag atacctatca ggtcaactac
    27121 accgtccaac ttaagcaaaa gttaaatgcc aatccttttg ccatctacaa tcgtatggtg
    27181 gtagagcagg aggcgggcta caatgcctat gtggaacatg acgagatggc agtgatttcc
    27241 atgagcccag agctcttttt tgagcaaaat gatcgcgagt tgacaacacg accaatgaag
    27301 gggacgactc agcgtggggt aactgaccaa gaagatcttg aacaggccag ttggttggaa
    27361 caggatccca aaaatcgctc tgaaaatatg atgattgtgg acctcttgcg caatgatatg
    27421 aaccgtattt ctgaagttgg gagcgagcac gtggagcgtc tgtgtcaggt agagcagtat
    27481 tcaactgttt ggcagatgac ttcgaccatc aagagtcagt tgcgagagga tgtggacctt
    27541 gttgaaatct tccgctcact ctttccttgt ggttccataa cgggtgcacc gaaaattgcg
    27601 acaatggaga ttatcaagga cttggagcct caaccgcgtg gagtctactg tggaacgatt
    27661 ggtctcttgc ttccaaatgg acgacggatt tttaatgtgg ccattcgtac cattcaactt
    27721 cacaaaggtc aagccattta tggagttggc ggagggatta cttgggatag cacatgggaa
    27781 tctgaatacc gagaggttca tcaaaaggca gcagttcttt atcgtaaaca agctcgcttc
    27841 caactgatta cgacagggga aatcagccag aagaacctgc tgtttgaaga tcaacatctg
    27901 gaaagactga gaaaagccag tcgttatttt gcctttcctt ttgatgcaga agacttggga
    27961 cacaagattg aggaagagtg tcaggattgt gaagctaatc aagattaccg cttgcgaatc
    28021 agccttagca aatctggaga gatagaagtc aatcgtcaag tattaacccc tctcagtaca
    28081 agcttttgtc aggcccaagt ctgtcttcag gaagctgctt tgaatcaatc ctttacctac
    28141 tttaaaacca ctcaccgacc gcatttgagc ctaggagaac aagagaagat ttaccacaat
    28201 aagtcaggag aactgcttga aacctctata ggaaatttgg ttctgaaaat cgctggaaaa
    28261 ctctacacac cgcctatccg acttggaatc ttgccaggaa tttaccgtca gcatttgcta
    28321 gaaacaggac aggtagaaga gaaagtcttg accttggcag atttaaccca agcagaagct
    28381 attcacggct gtaatgcagt gagaggcttg tatgaactaa gccttgagga gaactagatg
    28441 aaattgatat ttttgcatgg gttagggcag tcagcagagt cttggaaaga agttcggaat
    28501 ctactgacag attatccttc tgaggctatc gagttatttc cttctggagt tagtaactat
    28561 caacaagcta aagagcgagt ttatcagcac ctagctcaag agacagaacc ttttgttttg
    28621 attggattgt ccttaggtgc taccctgaca ctagaacttt ccagttacga tttaccaaat
    28681 cttcgagctt tgattctgtc aggatgcccg cttaaattag caggaaatat cctattttat
    28741 cttcaattgc tgatttttaa actacttccc aaaagagtat ttgaaaaaca gggagcagac
    28801 aagactctta tggtcggagt ttctgaggaa ttgaagacac ttgatttaac ggatatagca
    28861 gggacttgct cctatccaac cttactaatt tgtggcagca aagataaacc gaatcttagt
    28921 tctatgaaag ctctccatag actgttaaca gattcgcagt ttcagatcat tcctgatggc
    28981 cctcatgtct tgaataaggc aaaaccaaag gagtttgtag aaaagaccag aagtttcctt
    29041 gaattgctga aataagtttg ataaaataat attgaaatcg atatcataat tatgggagaa
    29101 agaaatgaat aaacgtctat ttttaaaaat gagtctggtg acgttgccaa ttttagcctt
    29161 gttttcacaa tcagttttgg cggaagaaaa catccatttt tcgagctgta aggaagcttg
    29221 ggcaaatgga tactcggata ttcacgaggg agaaccggga tattctgcca agttagaccg
    29281 agaccatgat ggtgtagctt gcgaattgaa aaatgctcct aagggtgctt tcaaagcaaa
    29341 acaatcaacg gtggctcaaa ctaacacaag ttcagcaaca acaagtggtt gggttgagca
    29401 ggatggcgct tggtactatt ttgatgaaaa tggaaatcca gtgaaaaatg catggcaggg
    29461 aaactattat ttgaaatcag atggtaaaat ggcacagggc gaatggattt atgattcttc
    29521 ctatcaagct tggtattatt tgacatcaga tggttcttat gcttacagta catggcaagg
    29581 aaattactat ctaaaatcgg atggtaaaat ggctgtcaat gaatgggttg atggtggacg
    29641 ttattatgtt ggcgctgacg gagtttggaa ggaaggtcaa gcaagtacag cttcttctag
    29701 taatgatagc aatagtgaat attctgctgc tttaggaaag gcaaaaagtt ataattcgtt
    29761 attccacatg tcaaaaaaac gtatgtatag acaattaact tctgattttg ataaattttc
    29821 aaatgatgca gctcaatatg ccattgatca ttagatgatt aacagatact ggtaggcgaa
    29881 aaaaattcct tataattcac ttacgcaaac ctttgcataa gtgaatgaat tttgatatac
    29941 tagtactgta agcattttca attactacac aatgaaaaag gagaatatga tgagtcaaaa
    30001 gattattggg attgaccttg gtggaacttc tatcaaattt gcaatcttaa caacagcagg
    30061 agaaatccaa ggaaaatggt caatcaagac caacattttg gatgagggaa gtcatatcgt
    30121 tgatgatatg attgagtcta ttcaacatcg tttggacttg cttggattgg cagcagcgga
    30181 cttccaaggc attggaatgg gatcaccagg tgtggttgac cgtgaaaaag ggactgttat
    30241 cggtgcctac aacttgaact ggaaaaccct tcaaccaatc aaacaaaaga ttgaaaaagc
    30301 cttgggcatt ccatttttca tcgataatga tgccaacgta gcagctcttg gtgagcgctg
    30361 gatgggtgct ggagataacc aaccagacgt tgtctttatg acactcggta ctggtgttgg
    30421 tggcggtatc gtcgcagaag gcaaattgct tcacggtgtt gctggtgcag caggtgagtt
    30481 gggccacatc actgttgact ttgaccagcc aattgcatgt acttgcggta agaaaggctg
    30541 ccttgagaca gttgcttcag caacagggat tgtcaacttg actcgtcgct atgccgatga
    30601 atacgaaggc gatgcagcct tgaaacgctt gattgataac ggagaagaag taactgctaa
    30661 gactgtcttt gagctcgcaa aagaagaaga cgaccttgct ttgattgttt accgtaactt
    30721 ctcacgttac ttgggtattg catgtgctaa cattggttca atcctaaacc catcaacaat
    30781 cgtcatcggt ggtggtgtgt cagctgcggg agaattcctt ctacaaggtg ttcaaaaagt
    30841 ttacgatgaa aatagtttcc cacaagtacg cacatctact aaattagctc ttgcaactct
    30901 aggaaatgac gctggagtta tcggagcagc atcacttgta ttgcaataag ataatgagag
    30961 aggaaaatgc taacctagag ttagtaatgt tcctctttta ttttttatgc tatactagtt
    31021 aggacaatga atgaggtgag agtaaatgac aaaagcagat acgattttta aagagaatat
    31081 tgaacgcatt ctaaaggaag gtgttttctc agagcaggct cgtcctaagt acaaggatgg
    31141 gactgttgct aattccaagt atgtaactgg tgccttttcc gagtacgact tatctaaagg
    31201 agaatttcct atcacaacct tgcgtcccat tgcaatcaaa tccgccatca aagaagttct
    31261 ctggatttac caagaccagt caaatagctt agaagtgctt aatgacaagt acaatgttca
    31321 ctactggaat gactgggaag ttggagacac gggaaccatt ggtgagcgct atggtgccgt
    31381 tgttaagaaa cacgacatta tcaataagct tcttaaacag ttggaaacca atccttggaa
    31441 ccgccgcaat attatttcgc tctgggatta ccaagctttc gaagaaacag atgggctgct
    31501 cccgtgcgcc tttcagacca tgtttgatgt tcggcgtgtt gatggggaaa tctatctgga
    31561 tgcgaccttg acccagcgct ccaatgatat gctggtggcc caccacatca acgctatgca
    31621 gtatgtggct ttgcagatga tgattgccaa acattttggt tggaaggttg ggaagttctt
    31681 ctatttcatc aacaacctcc atatctatga taatcaattt gaacaagctc aggaattgct
    31741 ccgtcgggag ccgtcaaact gccaaccacg cttggtttta aatgttcctg atgggactaa
    31801 tttctttgat atcaaagcag aagattttga gttggtggat tatgaccctg ttaagccgca
    31861 gttgaagttt gacctagcta tttaaaagaa tagaaaaaag aagttgagaa taatcccaac
    31921 ttcttttgtt tcttaacgtg atacgcggcg acgagctgct tttttacggt tttcttcgat
    31981 gaaagctgct ttttgctctt ctggttcgat tactttcttt ttaaatgcgt atactgcacc
    32041 tgcaacggca gcgacagttc ctgcgacacc tgttacaaga cctttagcga atcctttagc
    32101 catgagtctt cctcctttat attctcaatc agccatcctc ctcaagaggt cacatttttc
    32161 tgactgacct ttttgtgtta taataatagt aacgaaaaaa tgggaatttt tcaaggaaaa
    32221 aagatgaaaa caaaaataat tgtgattgtt ggaccgactg ctgttggaaa gacggctcta
    32281 gctattgaag ttgcaaagcg ttttaatggc gaagtggtta gtggagatag ccagcaagtc
    32341 tatcgaggac ttgatattgg gacggccaag gctagtccag aagagcaggc agctgttcct
    32401 catcatttaa tcgatgttag agagataacc gagtcttact cggcttttga ttttgtttca
    32461 gaagctaaga tgaccattga gggtattcac aaccgtggca agctagccat tatcgccggt
    32521 gggactggac tttatatcca gagcttgcta gaaggttacc acctaggtgg ggagactcct
    32581 catgaggaga ttttagctta tcgagctagt ttggagccat attcagatga ggaattagcc
    32641 catttggtgg agcaagcagg ccttgagatt ccccagttta atcgtcgtcg tgctatgcgt
    32701 gccttagaaa ttgcccattt tggtcaggat ttggaaaatc aagagacctt gtatgaaccg
    32761 ctgattatct gcttggatga tgaacgtagt caactttatg agcgtatcaa ccaccgagtg
    32821 gatttgatgt ttgaggctgg gcttttggat gaggccaagt ggctgtttga ccattcccct
    32881 aatgtacagg ctgctaaagg aattggctat aaggaactct ttccttattt ccgtggagag
    32941 cagaccttgg aggaagctag tgagagtctc aaacaggcga cccgtcgttt tgccaagcgt
    33001 cagctgacct ggttccgtaa tcgcatgcag gtcacctttt atcagattgg agaatctggt
    33061 gtacaagacc gcattttaag ccagatagag gagtttttag atgattgaaa cggagaaaaa
    33121 agaggagcga gtcctgctga ttggtgtgga attgcagggt atggacagtt ttgacctctc
    33181 catggaagaa ttggctagtt tagcgaaaac ggcaggggca gtcgttgtag atagctacag
    33241 acaaaaacgt gaaaaatatg attccaagac cttcgtcggc tctggtaagt tggaagagat
    33301 tgcgcttatg gtggatgcag aagaaatcac tactgtcatc gtcaacaacc gtctgacccc
    33361 aaggcagaat gtcaatctag aggaagttct cggtgttaag gtcattgacc gtatgcagtt
    33421 gattttggat atctttgcca tgcgggctcg aagccatgaa gggaagctcc aagtccacct
    33481 agcccaactc aaatacctct tgcctcgctt ggttggtcag gggattatgc tcagccgtca
    33541 ggcaggggga attggttccc gtggtcctgg tgaaagccaa ctggagctga accgtcgtag
    33601 cgttcgcaat caaatcacgg atatcgagcg ccagctcaag gtggttgaga aaaatcgggc
    33661 gactgtcaga gaaaaacgtt tggagtctag cacctttaag attggtttga ttggttatac
    33721 caatgctggg aaatcaacta tcatgaacat cttgaccagt aagacccagt atgaagcaga
    33781 tgagctcttt gcgactctgg atgcgacaac caagagtatt catctgggag gcaatctcca
    33841 agtaactttg acagatactg ttggctttat ccaagatttg ccgacagagt tggtgtccag
    33901 tttcaagtca accttggaag aaagcaagca tgtggacctt ctggttcatg ttatcgatgc
    33961 tagcaatcct taccacgagg agcatgaaaa aacggttctc tccatcatga aagacctgga
    34021 catggaagat attcctcact tgacgcttta taataaagcg gatttggtgg aggatttcac
    34081 gcctacccaa acgccatata ccctcatttc tgccaagtct gaggacagtc gtgaaaactt
    34141 gcaagcatta ttgctagata agattaagga aatttttgaa gcatttaccc tgcgagtgcc
    34201 tttttcaaag tcctacaaga ttcatgattt agagagtgtt gcaattctgg aagaacgtga
    34261 ttatcaggaa gacggcgaag tgattacagg ctacatttcc gagaaaaata aatggaggtt
    34321 agaagaattt tatgactgat attaaaactt tggctctaaa gtatggaggt tatacaagtc
    34381 tggacaaggt ctatctggat cagcttctag ctggtaaaac agagcaggag cagttaacac
    34441 ttattacacc tccgccaagt gttgttaatg cctactttgc agaactctat cagaaaaaaa
    34501 gtcctcaggc tgcgacggat tattttgcag aactcagtca ggaactgaat ctttacaata
    34561 ctgagccaag tttcacccta gaaaataagc cttttattcg gcttaatctg tctggtaaat
    34621 cctttggttt ttgttatgag agtgacggct ttggtcggat tttctctgaa aatgaggaga
    34681 aaatctcgga tgacttgctc tttgaaattg cgcaaatttt ctcccatcaa ttagtctttg
    34741 aagagtctgg taagatttac atgaagcctg tcaaggacga ggaagttgtc agcctagaaa
    34801 gtctcacagc tttgactgat ttggaaagat tggctgatgg tcgaaaacgt ctcaaaggct
    34861 acagccaaga ggatttattg caagaagctg ctgctttttc tggcaaacgc tatttccgat
    34921 cggaaaaccg cacagccatg ttatatattg attaattaga aagtatcgaa tggatattca
    34981 atttttagga acgggggctg gtcagccctc taaagcccgc aacgtttcaa gtctcgccct
    35041 gaaacttttg gacgagatta acgaagtttg gctctttgac tgtggagaag gtactcaaaa
    35101 tcgcattctg gaaaccacaa ttcgaccacg taaggtcagc aaaatcttta ttactcatct
    35161 gcatggagac cacatttttg gtttgccagg tttcctttct agccgtgcct ttcaggccaa
    35221 tgaagagcag acagatttgg aaatctacgg acctcaagga atcaagtcat ttgtcttaac
    35281 cagccttcgt gtgtcaggtt ctcgtctgcc ctaccgcatt catttccatg agtttgacca
    35341 agattctctg ggtaaaattc ttgaaaccga taaattcact gtgtatgcag aggagctgga
    35401 ccacactatt ttctgtgttg gctatcgtgt catgcaaaag gatctagaag ggacgctgga
    35461 tgctgaaaaa ctcaaggctg ctggtgttcc gttcggcccg ctttttggta aaatcaaaaa
    35521 tggccaggat cttgttttgg aagacggaac tgaaatcaag gcagcagact atatctcagc
    35581 gccacgtcca ggtaagatta tcactatttt aggagacact cgaaaaacgg atgccagtgt
    35641 gcgtctggct gtcaatgcag atgtcctagt tcatgagtcc acttatggca agggtgatga
    35701 aaaaattgct cgtaaccatg gtcactcaac taatatgcaa gctgcacaag tagcggtaga
    35761 agcaggtgcc aaacgcctcc tactcaacca tatcagtgcc cgtttcctct caaaagatat
    35821 aagcaaactc aagaaagacg ctgccacaat ttttgaaaat gtccatgtgg tcaaagactt
    35881 ggaagaagtg gaaatctagc agtcacagaa aggataagta tgcctactat tctcattacg
    35941 ggagctagcg gtggtctagc tcaagaaatg gtcaaactcc tgcccaatga ccaactcatc
    36001 ttgcttggta gaaataagga aaaattagcc caactctacg gaaattattc ccatgcagaa
    36061 ttgattgaaa ttgatattac cgacgattca gccctagaag ctctggtaac tgatctttat
    36121 ctccgctatg gcaagattga tgtcttgatt aacaacgctg gttacgggat ttttgaggga
    36181 tttgaccaaa ttgctgataa agacattcac cagatgtttg aggtcaatac ctttgccctg
    36241 atgaatctgt ctcgtcacct tgcggctcgt atgaaggaaa gcagcaaagg gcatatcatc
    36301 aacatcgtca gcatggcagg tctaatagct actggcaagt ctagtcttta ctcagcgacc
    36361 aagtttgcgg ctattggttt ttcaaatgct ctgcgactcg aacttatgcc ctatggagtc
    36421 tatgtgacaa cagtcaatcc aggtccaatc cgaacaggat tttttgacca agctgaccca
    36481 gatggaactt atcttaaatc ggttgaccgc ttcctcttag aggcagatgc agtggctaaa
    36541 aagattgtca agattatagg caaaaataaa cgagaactca atctcccgat tttgttgaac
    36601 ctagcccata agttttatac tctctttccc aagctagctg ataagttggc aggggaaact
    36661 tttaattata agtaaaaaga accaatgtgc aggttgttgc tagcctacat attggttctt
    36721 atcatttctc aactatcaaa ctgaacttct tctagtagat agtcgataaa gcgttcgccc
    36781 atcttagata ggctggtttt ctcatgctgg atatagacca gctcaatcgg gtcatcaata
    36841 tccagtggga tagaaacaat attgtctccg tttaggttgc tgttcaaaat ccctgtcgca
    36901 atggtataac catccaaacc aatcaagaga ttaaagaggg tggcacggtc actgaccaca
    36961 atggattttt tgtggtgttc ttgagaaaga atctcttctg aaaagtagaa ggagttgtgc
    37021 gtcccttggt catagctgag gtaagggaaa ttctccaaat cagacagttt caccttgtct
    37081 ttctttgcca gagggttggt cttgctgaca aagatatgcg gttgcgctgt gaagagatgg
    37141 tgggctagca ggtgattgtc atccagcatc ttggttaaaa catcacggtt gtaactgttt
    37201 aagaagagga ccccgacctc actgcggaag ttcttgacgt cgtcgataat ctcccaagtc
    37261 cgagtttcac gaaggaagag ttcgtatttc tccatatcgc ttttcttgag caaagagaca
    37321 aaggcattga ccacaaaggc atagtgttga gacgaaacgc taaatagttc gcggtgggcg
    37381 acaggatttt tatagcgttc ctccagaagc tgggtctgct cgacaacctg acgggcataa
    37441 gagagaaact ccatcccatc acgggtcaag gtgattccct tgggattgcg gataaagatc
    37501 tcaatgccca tttcattttc caaatctcgc actgcattgg agagacttgg ctgagtgata
    37561 aagagttgct tggctgcctc attcatggag ccagtttcga cgattttgat aatataatgt
    37621 aattgttgaa ttctcatgtt tttattgtac catacttgcc ttagaatcgc taaagaagat
    37681 aggaaaataa ggagcattgt gtgagtctct tcttgaaaaa aacacaaaat tttgctagaa
    37741 tgagtcttat gaaaacattc tatgatgtgc agcaattcct caaacgattt ggtattattg
    37801 tttacatggg aaaacgctta tatgatattg aactgatgaa gttggaacta tctcggattt
    37861 acgatgcagg gttgatggat aaattagact atctagaggc agaagcagtt cttcgcagag
    37921 agcacaaggt agaattggat tatattgaga aaaatggaga aaagaactaa tggttacttg
    37981 gattttgtgg gcacttatac tagcaatgtt ggcgtggatg ggctttaact atcttcgtat
    38041 tcgccgtgcg gctaaaattg tggacaatga ggagtttgaa gccttgattc gtacgggtca
    38101 attgattgat ttgcgcgacc cagcagaatt ccacagaaaa catatccttg gtgcacgcaa
    38161 tattccttca agtcagttga aaactagtct tgcagccctt cgtaaagata aacctgtcct
    38221 tctctacgaa aaccaacgtg cgcaacgagt tacaaatgca gctctttact tgaaaaaaca
    38281 aggtttttct gagatttata tcctttctta tggcttggat tcttggaaag ggaaagtgaa
    38341 gactagctaa ccttatttcc aaattagtcg aatgatagga tgaagatatt gttttatagt
    38401 ataattaatt tgtataaaat ttaaagagtt cttagaacat gaaagaaaag aagaaaaatc
    38461 cactttttgt gggtatcttg tcgattattc ttggtttgtt atttccaatt gtaggcctga
    38521 ttttgggtat cataggattg gtcttggcta tttcatacca aaaagagtca caattagact
    38581 ataaaataga aaagattctt aatatcttag gaattgtgat ttctgtagtt aactggattg
    38641 tagcaatcgc cttaattttt cggtaaataa aagaaaaaag ctttagtttt aaagcttttt
    38701 ttgttttatc gactcatctc taaatagttt ttaatgattt gtaactcttt ttggttcaaa
    38761 gggcggtagt taccttctgc taaatctggg ttcaatgtga agtccccaaa ttggattctc
    38821 ttgaggctag tcaccttaac accaaccgag aggaacattt tcttgatttg atgaaatttt
    38881 ccttctgaaa tggtgataga ggcttgactg agggaaggac ttgcagatag aatctctagt
    38941 cttgcaggtt tacagatagt gccatctaaa aagacaattc ccttttgaaa ggtttggata
    39001 tggtcaggtg ttagaagtcc attaacctca acttgataag tcttatcgac atgatattgg
    39061 ggatggagga gttggaagcc caagggaccg ttatcggtca agagaaggag tcctgttgta
    39121 tctcggtcca gtcggccgac ggcatagagc ttgtcagact ggatgtcaga tggaagcaag
    39181 tccatgacgg tcggaagttc tttgtctttg ttggctgtaa cgacaccagc aggtttatga
    39241 agcataagat aggtgtgttc atagccttga atgattcgat cctgaaaaag gagttcttgt
    39301 agtcctgtat cgatattttg agctagggag cgggctgggc aaccatcgac tagaatgtcc
    39361 cctctgagga gtgcttgctt catggccttt cggctaactt tttcttgggc taataaatta
    39421 tctaaacgca tactgtgcct atcttatcat aagtcgcttg ctttgaaaag gcttgacgtg
    39481 aaaagaaaag cggagtgaaa acatttacac ttgcttgagt tatgttattt acttgaaatt
    39541 atggtataat cgttcagtta gaaaataaat tttgaatatt atagaggaaa tcatgacaaa
    39601 attaagagaa gatatccgta acattgcgat tatcgcccac gttgaccacg gtaaaacaac
    39661 cctggttgac gaattattga aacaatcaga aacgcttgat gcacgtactg aattggcaga
    39721 gcgtgctatg gactcaaacg atatcgaaaa agagcgtggg attaccatcc ttgctaaaaa
    39781 cactgccgtt gcttacaacg gaactcgtat caacattatg gacacaccag gacacgcgga
    39841 ctttggtgga gaagttgagc gtatcatgaa aatggttgac ggtgttgtct tggtcgtaga
    39901 tgcctatgaa ggaaccatgc cacaaactcg tttcgtattg aaaaaagcct tggaacaaga
    39961 ccttgtccca atcgtggttg ttaacaaaat cgataagcca tcagctcgtc cagcagaagt
    40021 agtggatgaa gtcttggaac ttttcatcga gcttggtgca gatgacgacc agcttgattt
    40081 cccagtggtt tatgcttcag cgatcaacgg aacttcttca ttgtcagatg atccagctga
    40141 ccaagaagcg actatggcac caatctttga cacgattatc gaccatatcc cagctccagt
    40201 agataactca gatgagcctt tgcagttcca agtgtcactt ttggactaca atgactttgt
    40261 tggacgtatc ggtatcggtc gtgtcttccg tggtacagtt aaggttgggg accaggttac
    40321 cctttctaaa cttgacggta caactaaaaa cttccgtgtt acaaaactct tcggtttctt
    40381 tggtttggaa cgtcgtgaaa tccaagaagc caaagcgggt gacttgattg ccgtttcagg
    40441 tatggaagac atctttgtcg gtgaaaccat cactccgaca gatgcagtag aagctcttcc
    40501 aatcctacac atcgatgagc caactcttca aatgactttc ttggtcaaca actcaccatt
    40561 tgctggtaaa gaaggtaaat gggtaacttc tcgtaaggtg gaagaacgct tgcaggcaga
    40621 attgcaaaca gacgtttccc ttcgtgttga cccaactgat tcaccagata aatggactgt
    40681 ttcaggacgt ggagaattgc acttgtcaat ccttatcgaa acaatgcgtc gtgagggcta
    40741 tgaacttcaa gtatctcgtc cagaagttat cgtaaaagaa atcgacggtg ttaaatgtga
    40801 accatttgaa cgtgtacaaa tcgacactcc agaagaatac caagggtctg ttatccaaag
    40861 cctttctgaa cgtaaaggtg aaatgttgga tatgatttca actggtaatg gtcaaactcg
    40921 tttggtcttc cttgttccag cgcgtggttt gattggatac tcaactgagt tcttgtcaat
    40981 gactcgtggt tacggtatca tgaaccatac cttcgaccag tacttgccat tgattccagg
    41041 ggaaattggt ggacgtcacc gtggtgccct tgtttctatc gatgctggta aggctacaac
    41101 ttactcaatc atgtctatcg aagaacgtgg tacgatcttt gtcaacccag gtactgaggt
    41161 ttatgaagga atgatcatcg gcgaaaactc tcgtgaaaat gacttgacag ttaacatcac
    41221 gaaggcaaaa caaatgacca acgttcgttc agctactaag gaccaaacag ctgttatcaa
    41281 gacgcctcgt atcttgacac ttgaagagtc tcttgagttc ttgaacgacg atgagtacat
    41341 ggaagtaacg cctgagtcta tccgtttgcg taaacaaatc cttaacaaag cagagcgtga
    41401 gaaagctaac aagaagaaaa aatcagctga ataagagcta gaaagagaga aagatggtct
    41461 atttagtcct aggaatttta ctgctcctac tctatgtatt tgcgacacca gaaagcatta
    41521 aagggacagt caatatcgtc gctatggtat gtattttagt ggcactcttg attttattgg
    41581 ttctatcttt tctgaaaatt tttcaattac caacagaaat attcctagca atagccatgt
    41641 tgatcctagc ttactttagt gtgagagaca tcacactcat gccagtcaaa aaaggtaaaa
    41701 gaagataaaa aagagagttt cggctctctt tttgctagga attttacaat agtttgaatt
    41761 gctttttagt taacaccatc tcttttgttt ggataatatg aatgggaaaa acttgaataa
    41821 aattctttaa tggaatctat tttatatttg gttagtactg gttgcatatt attttgaaat
    41881 aa
//
LOCUS       DAVKJD010000011        40356 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKJD010000011 DAVKJD010000000
VERSION     DAVKJD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40356
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.011"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            5..75
                     /locus_tag="VW157_000601"
     tRNA            5..75
                     /locus_tag="VW157_000601"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VW157_000602"
     tRNA            110..183
                     /locus_tag="VW157_000602"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="VW157_000603"
     tRNA            266..355
                     /locus_tag="VW157_000603"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            366..439
                     /locus_tag="VW157_000604"
     tRNA            366..439
                     /locus_tag="VW157_000604"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Met,seq:cat)
     gene            443..515
                     /locus_tag="VW157_000605"
     tRNA            443..515
                     /locus_tag="VW157_000605"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Phe,seq:gaa)
     gene            528..608
                     /locus_tag="VW157_000606"
     tRNA            528..608
                     /locus_tag="VW157_000606"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Tyr,seq:gta)
     gene            613..683
                     /locus_tag="VW157_000607"
     tRNA            613..683
                     /locus_tag="VW157_000607"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:645..647,aa:Trp,seq:cca)
     gene            698..770
                     /locus_tag="VW157_000608"
     tRNA            698..770
                     /locus_tag="VW157_000608"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:731..733,aa:His,seq:gtg)
     gene            781..852
                     /locus_tag="VW157_000609"
     tRNA            781..852
                     /locus_tag="VW157_000609"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:813..815,aa:Gln,seq:ttg)
     gene            862..945
                     /locus_tag="VW157_000610"
     tRNA            862..945
                     /locus_tag="VW157_000610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:896..898,aa:Leu,seq:caa)
     gene            1052..2329
                     /locus_tag="VW157_000611"
     CDS             1052..2329
                     /locus_tag="VW157_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU9551949.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2405..3889
                     /locus_tag="VW157_000612"
     CDS             2405..3889
                     /locus_tag="VW157_000612"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9551950.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALRERLAA
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQAVTDDATKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAADNMQAAVEAYLGL"
     gene            3950..5230
                     /locus_tag="VW157_000613"
     CDS             3950..5230
                     /locus_tag="VW157_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9551951.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLLGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5234..5854
                     /locus_tag="VW157_000614"
     CDS             5234..5854
                     /locus_tag="VW157_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9551952.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFNYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWVDESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6020..7162
                     /locus_tag="VW157_000615"
     CDS             6020..7162
                     /locus_tag="VW157_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9551953.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETTRPVEEATPQETTPAEKQETQASPQAASVVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVAERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7587..8012
                     /locus_tag="VW157_000616"
     CDS             7587..8012
                     /locus_tag="VW157_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9551954.1"
                     /translation="MIEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAVKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            8023..8391
                     /locus_tag="VW157_000617"
     CDS             8023..8391
                     /locus_tag="VW157_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU9551955.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            8498..9142
                     /gene="pcp"
                     /locus_tag="VW157_000618"
     CDS             8498..9142
                     /gene="pcp"
                     /locus_tag="VW157_000618"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9551956.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVAINQDDARTSDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFSYVK
                     AGFMHIPYMMEQVVNRPTTPAMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(9170..10027)
                     /locus_tag="VW157_000619"
     CDS             complement(9170..10027)
                     /locus_tag="VW157_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9551957.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLAGLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQRFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10157..11299
                     /gene="tgt"
                     /locus_tag="VW157_000620"
     CDS             10157..11299
                     /gene="tgt"
                     /locus_tag="VW157_000620"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9551958.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11397..13214
                     /locus_tag="VW157_000621"
     CDS             11397..13214
                     /locus_tag="VW157_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9551959.1"
                     /translation="MRIKWFSLIRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     ASVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13367..14518
                     /gene="nagA"
                     /locus_tag="VW157_000622"
     CDS             13367..14518
                     /gene="nagA"
                     /locus_tag="VW157_000622"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9551960.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14681..15739
                     /locus_tag="VW157_000623"
     CDS             14681..15739
                     /locus_tag="VW157_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9551961.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLDTAVSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15890..16255
                     /locus_tag="VW157_000624"
     CDS             15890..16255
                     /locus_tag="VW157_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9551962.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16331..17272
                     /locus_tag="VW157_000625"
     CDS             16331..17272
                     /locus_tag="VW157_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9551963.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17220..18236
                     /locus_tag="VW157_000626"
     CDS             17220..18236
                     /locus_tag="VW157_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9551964.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVIQMRAGLSQGKSFSEMMESLGFSSTIITQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18238..18564
                     /gene="comGC"
                     /locus_tag="VW157_000627"
     CDS             18238..18564
                     /gene="comGC"
                     /locus_tag="VW157_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEU9551965.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKDDGRITEEQAKAYKEYHDKNGEA
                     NRKVND"
     gene            18557..18961
                     /locus_tag="VW157_000628"
     CDS             18557..18961
                     /locus_tag="VW157_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9551966.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18966..19226
                     /locus_tag="VW157_000629"
     CDS             18966..19226
                     /locus_tag="VW157_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551967.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19189..19650
                     /locus_tag="VW157_000630"
     CDS             19189..19650
                     /locus_tag="VW157_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9551968.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS"
     gene            19628..20041
                     /locus_tag="VW157_000631"
     CDS             19628..20041
                     /locus_tag="VW157_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9551969.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQDFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEIATDSSEKVEKKKSEEKPEKKENS"
     gene            20178..20772
                     /locus_tag="VW157_000632"
                     /pseudo
     CDS             20178..20772
                     /locus_tag="VW157_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20833..21786
                     /locus_tag="VW157_000633"
     CDS             20833..21786
                     /locus_tag="VW157_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9551970.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21837..23027
                     /locus_tag="VW157_000634"
     CDS             21837..23027
                     /locus_tag="VW157_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9551971.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGCSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKYIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23176..23547
                     /gene="rnpA"
                     /locus_tag="VW157_000635"
     CDS             23176..23547
                     /gene="rnpA"
                     /locus_tag="VW157_000635"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9551972.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23522..24346
                     /locus_tag="VW157_000636"
     CDS             23522..24346
                     /locus_tag="VW157_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9551973.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24365..25351
                     /locus_tag="VW157_000637"
     CDS             24365..25351
                     /locus_tag="VW157_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9551974.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSRTFYVTINVNDYVEHRAEVLQTYAQKL
                     ATRVLEEGRNHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25402..26025
                     /locus_tag="VW157_000638"
     CDS             25402..26025
                     /locus_tag="VW157_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9551975.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMIALRLYGNPSDF
                     GISLEVSFIERKKDEQTLGKQAKVLEIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26156..27613
                     /locus_tag="VW157_000639"
     CDS             26156..27613
                     /locus_tag="VW157_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9551976.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27636..27917
                     /locus_tag="VW157_000640"
     CDS             27636..27917
                     /locus_tag="VW157_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9551977.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27994..28479
                     /locus_tag="VW157_000641"
     CDS             27994..28479
                     /locus_tag="VW157_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9551978.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELDDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28496..29161
                     /locus_tag="VW157_000642"
     CDS             28496..29161
                     /locus_tag="VW157_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9551979.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDIFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29165..30028
                     /locus_tag="VW157_000643"
     CDS             29165..30028
                     /locus_tag="VW157_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9551980.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFIEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDSVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHIKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            30030..30734
                     /locus_tag="VW157_000644"
     CDS             30030..30734
                     /locus_tag="VW157_000644"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9551981.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVTYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVEPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30913..32586
                     /locus_tag="VW157_000645"
     CDS             30913..32586
                     /locus_tag="VW157_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9551982.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLCIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32698..33789
                     /gene="ulaG"
                     /locus_tag="VW157_000646"
     CDS             32698..33789
                     /gene="ulaG"
                     /locus_tag="VW157_000646"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9551983.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33903..35879
                     /gene="tkt"
                     /locus_tag="VW157_000647"
     CDS             33903..35879
                     /gene="tkt"
                     /locus_tag="VW157_000647"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9551984.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            35996..36295
                     /gene="yajC"
                     /locus_tag="VW157_000648"
     CDS             35996..36295
                     /gene="yajC"
                     /locus_tag="VW157_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9551985.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36342..36770
                     /locus_tag="VW157_000649"
     CDS             36342..36770
                     /locus_tag="VW157_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9551986.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQQYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36772..37182
                     /locus_tag="VW157_000650"
     CDS             36772..37182
                     /locus_tag="VW157_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551987.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37482..40133
                     /gene="adhE"
                     /locus_tag="VW157_000651"
                     /gene_synonym="adhC"
     CDS             37482..40133
                     /gene="adhE"
                     /locus_tag="VW157_000651"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9551988.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRKLAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12258 a         7246 c         9088 g        11764 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attaacgcgg gatggagcag ctcggtagct cgtcgggctc ataacccgaa ggtcgtaggt
      421 tcaaatcctg ctcccgcaat aaggctcggt agctcagttg gtagagcaat ggattgaagc
      481 tccatgtgtc ggcggttcga ttccgtctcg cgccatttat atattttgga agggtagcga
      541 agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt cgaatccctc
      601 cccttccatt ttacgggcat agtttaaagg tagaactaag gtctccaaaa ccttcagtgt
      661 gggttcaatt cctactgccc gtgttaatag aattatggcg ggtgtggtga agtggttaac
      721 acaccagatt gtggctctgg catgcgtggg ttcgatcccc atcactcgcc tattttatat
      781 tggggtatag ccaagcggta aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc
      841 cagctacccc agttactatt tgccggcgtg gcggaattgg cagacgcgct ggactcaaaa
      901 tccagtgtcc gcaaggacgt gccggttcga ccccggccgc cggtatagta tagtgttagg
      961 aacgttgtta ttcttcgttc cttttttata ttatttttgg tataattata gttattcaaa
     1021 ttttatttag attaagaaag tgtaggggag tatgtcttgt tctatcgatt tattaaaaca
     1081 tcggtatttg aaaaatatta aagaaaatcc tgaattgttt gtcggaattg agttggagta
     1141 tcctgttgca agtttagaag gggatgctac agatgttgaa gttatgaagg atctatttca
     1201 ttatttagtt tctactttgg atctcaccgt agcaaaggta gatgattttg gcaatctgat
     1261 ccagttagta gatccgataa gtcaggatgc tattttattt gaagtttcct atacaacgat
     1321 tgagtttgca tttggtaagg ctgaaacgat tcaagaggtc gaaaatcgtt tcaataatta
     1381 tatgaatgta attcagagaa agttagctga atcaaatcat gctattgttg gctgtggtat
     1441 ccatcccaac tgggataaaa atgagaattg tccagtggct tatccacgct atcagatgtt
     1501 gatggattat ttgaatttga gtagaaatat tattaaatca gatttacatc atttccctga
     1561 atatggtact tttatctgtg ggagccaggt tcagctggat atttcaaaaa ccaactactt
     1621 acgggtgatt aatgctttta ctcaaattga agcggctaag gcttatttat ttgcaaactc
     1681 tgaattttcg ggtgcggatt gggatacgaa aatttcaagg gatattttct gggaagaatc
     1741 tatgcatggt atctatccag agaatgttgg ggtcaatgct agactcctta atgatgaagc
     1801 tgattttttt gactatctaa atcattctgc gatttttact gcggaacgtg atgggcagac
     1861 ctattatttt tatcctattc aggctgggga ctatttggct acgtccgaaa tccaagcatt
     1921 tgctctgaat ggggatgagg ttattattta cccccaagag aaggattttg aaactcatcg
     1981 tagttaccag taccaagatt taacgactcg aggaacagtt gagtttcgta gtgtgtgtac
     2041 acagccactt gataggactt ttgcttctgc agcttttcac ttgggattat tggttaattt
     2101 agacaagtta gaagcttact tagaaacagc acctttcttt aaagtatttg gttatgatta
     2161 caagtcttta aggagacaat tttctaagaa aaatcttaca gatgaggaag aaactacgat
     2221 tattgaattt tccaaagact tactcctact agctgaggag ggactagtgg tgagaaataa
     2281 ggaagaaatg acctatttac agcctttgag agaagaattg agcctataat ttctcttata
     2341 aagggagaat tttctgaaaa atcatgatat aatggacgag actatagata aaggatagag
     2401 agtaatgaca ttagtttatc aatcaacgcg tgatgccaac aatacagtaa ctgccagcca
     2461 agcaattttg caaggtttgg cgacggacgg cggtttgttt acaccggtta cttatccaaa
     2521 ggtagatttg gactttgaca aattgaaaga tgcttcttac caggaagttg ctaagctagt
     2581 tttgtcagca tttttagatg actttacagc tgaggagttg gactactgta tcaacaatgc
     2641 ctacgatagc aaatttgata ctccagctat tgcaccatta gtgaaattag atgggcaata
     2701 caatttggaa cttttccatg gttcaacgat tgcctttaag gatatggcct tgtctatttt
     2761 gccatacttt atgacgactg ctgctaagaa acatggtttg gagaacaaga ttgttatctt
     2821 gacagcgaca tctggtgaca cggggaaagc tgctatggcg gggtttgcgg atgtgcctgg
     2881 tactgagatt atcgtctttt atccaaaggg tggtgtcagc aaggtacaag agttgcaaat
     2941 gaccactcag aatggtgaca atactcatgt tatcgctata gatggtaact ttgacgatgc
     3001 gcaaaccaac gtgaagcaca tgtttaacga cgtggctctt cgtgaaagat tagcggccaa
     3061 caagttgcaa ttttcatctg ctaactctat gaatatcggt cgtttggtgc cacagattgt
     3121 ttattatgtt tatgcctatg ctcagttggt taagactggt gaaattgtag ctggtgaaaa
     3181 gattaacttc acagtaccaa caggaaactt tggaaatatc ttggctgcct tttatgccaa
     3241 acaaatcggc ctaccagttg gcaagctaat ctgtgcttca aatgacaaca atgttttgac
     3301 agacttcttt aaaacacgtg tctatgacaa aaaacgtgag tttaaggtaa caaccagtcc
     3361 atctatggat atcttggtat cttcaaactt ggagcgcttg attttccatc ttttgggaaa
     3421 taatgctgaa aagacagctg aacttatgaa tgccttgaac acgcaaggac aatataagtt
     3481 gacagacttt gatgcagaga ttttggacct ctttgcagct gaatatgcga ctgaggaaga
     3541 aacagcggca gaaatcaagc gtgtttatga gtcagattct tatattgagg atccacatac
     3601 ggcggttgcc tcagcagttt atagaaaata ccaagcggtt actgatgatg cgactaagac
     3661 agtgattgct tcaacagcta gtccatacaa gttcccagta gttgcagtag aagctgtaac
     3721 tggaaaagca ggtttaacag actttgaagc cttggctcaa ttacatgaaa tctcaggcgt
     3781 tgcagtgcca ccagcagttg atgggcttga aatagctcca attcgtcaca agacaacagt
     3841 ggcagctgat aacatgcaag cagcggttga ggcttattta ggactttaag acagagggag
     3901 caaactcggt tgggaaacca actgagtttc ttttcatcag gaggagagat tgtttaagaa
     3961 aaataaagac attcttaata ttgcattgcc agctatgggt gaaaactttt tgcagatgct
     4021 aatgggaatg gtggacagtt atttggttgc tcatttagga ttgatagcta tttcaggggt
     4081 ttcagtagct ggtaatatta tcaccattta tcaggcgatt ttcatcgctc tgggagctgc
     4141 tatttccagt gttatttcaa aaagcatagg gcagaaagac cagtcgaagt tggcctatca
     4201 tgtgactgag gcgttgaaga ttaccttact attaagtttc cttttaggat ttttgtccat
     4261 cttcgctggg aaagagatga taggactttt ggggacggag agggatgtag ctgagagtgg
     4321 tggactgtat ctatctttgg taggcggatc gattgttctc ttaggtttaa tgactagtct
     4381 gggagccttg attcgtgcaa cgcataatcc acgtctgcct ctctatgtta gttttttatc
     4441 caatgccttg aatattcttt tttcaagtct agctattttt gttctggata tggggatagc
     4501 tggtgttgct tgggggacaa ttctgtctcg tttggttggt cttgtgattt tgtggtcaca
     4561 attaaaactg ccttatggga agccaacttt tggtttagat aaggaactgt tgaccttggc
     4621 tttaccagca gctggagagc gacttatgat gagggctgga gatgtagtga tcattgcctt
     4681 ggtcgtttct tttgggacgg aggcagttgc tgggaatgca atcggagaag tcttgaccca
     4741 gtttaactat atgcctgcct ttggcgtcgc tacggcaacg gtcatgctgt tggcccgagc
     4801 agttggagag gatgattgga aaagagtcgc tagtttgagt aaacaaacct tttggctttc
     4861 tctgttcctc atgttgcccc tgtcctttag tatatatgtc ttgggtgtac cattaactca
     4921 tctctatacg actgattctc tagcggtgga ggctagtgtt ctagtgacac tgttttcact
     4981 acttggaacc cctatgacga caggaacagt catctatacg gcagtctggc agggattagg
     5041 aaatgcacgc ctcccttttt atgcgacaag tataggcatg tggtgtatcc gcattggaac
     5101 aggatatctg ctggggattg tgcttggttg gggcttgcct ggtatttggg cagggtctct
     5161 cttggataat ggttttcgct ggttatttct acgctatcgt taccagcgct atatgagctt
     5221 gaaaggatag gaaatgcaaa aaacagcttt tatttgggat ttagacggga ctttattgga
     5281 ctcttacgaa gcgattttat cagggattga ggagactttt gctcagtttt ctattcctta
     5341 tgataaggag aaggtgagag agtttatctt caattattcg gtgcaagatt tgcttgtgcg
     5401 ggtggcagaa gatagaaatc tggatgttga ggtgctaaat caggtgcgtg cccagagtct
     5461 ggctgagaag aatgctcagg tagttttgat gccaggtgcg cgtgaggtgc tagcttgggt
     5521 agacgaatca ggaattcagc agtttatata tactcataag gggaacaacg cttttaccat
     5581 tctcaaggac ttgggggtgg aatcctattt tacagagatt ttaaccagtc agagtggctt
     5641 tgtgcggaag ccaagtccag aagcggctac ctatctgcta gataagtatc agttgaattc
     5701 tgataatact tattatatag gggatcggac tctggatgtg gaatttgccc agaatagtgg
     5761 gattcaaagt atcaactttt tagagtctac ttatgaaggg aatcacagga ttcaagcgtt
     5821 agcagatatt tcccgtattt ttgagactaa gtgataaaaa gattgtgtca gttttgtgac
     5881 agagacctaa caaactattt caagtaacct agtttgttac aaggaataga cagttctgtt
     5941 aaataggccc gagagggctt tttttctaca ttttttgtgt tatgatagac aggtactcat
     6001 ttgaaaggaa tttgaaagaa tgaagaaaag aatgttatta gcgtcaacag tagccttgtc
     6061 atttgcccca gtattggcaa ctcaagcaga agaagttctt tggactgcac gtagtgttga
     6121 gcaaatccaa aacgatttga ctaaaacgga caacaaaaca agttataccg ttcagtatgg
     6181 tgataccttg agcaccattg cagaagcctt gggtgtagat gtcacagtgc ttgcgaatct
     6241 gaacaaaatc actaatatgg acttgatttt cccagaaact gttttgacaa cgactgtcaa
     6301 tgaagcagaa gaagtaacag aagttgaaat ccaaacacct caagcagact ctagtgaaga
     6361 agtgacaact gcgacagcag atttgaccac taatcaagtg accgttgatg atcaaactgt
     6421 tcaggttgca gacctttctc aaccgattgc agaagctcca aaagaagtag catcaagttc
     6481 agaagttaca aagacagtga ttgcttctga agaagtggca ccatctacgg gcacttctgt
     6541 cccagaggag caaacggccg aaacaactcg cccagttgaa gaagcaactc ctcaggaaac
     6601 gactccagct gagaagcagg aaacacaagc aagccctcaa gctgcatcag tagtggaagc
     6661 aactacaaca agttcagaag caaaagaagt agcatcatca aatggagcta cagcagcagt
     6721 ttctacttat caaccagaag aaacgaaagt aatttcaaca acttacgagg caccagctgc
     6781 gcccgattat gctggacttg cagtagcaaa atctgaaaat gcaggtcttc aaccacaaac
     6841 agctgccttt aaagaagaaa ttgctaactt gtttggcatt acatccttta gtggttatcg
     6901 tccaggagac agtggagatc acggaaaagg tttggctatc gactttatgg tagcagaacg
     6961 ttcagaatta ggggataaga ttgcggaata tgctattcaa aacatggcca gccgtggcat
     7021 tagttacatc atctggaaac aacgtttcta tgctccattc gatagcaaat atgggccagc
     7081 taacacttgg aacccaatgc cagaccgtgg tagtgtgaca gaaaatcact atgatcacgt
     7141 tcacgtttca atgaatggat aaaccagatt tgataatata atgtttacga atgagatcta
     7201 gcttacgtga tgggaagcga ttctcgttcg ttttttcttt gtcatactct tcgaaaatct
     7261 cttcaaacca cgtcagtttt atctgaaacc tcaaagctgt gctttgagca acctgcgact
     7321 agcttcctag tttgctcttt gattttcatt gagtatcaat ttgaatggaa aatgggaaga
     7381 taccatattg taataatcta agcaacattc ttgcaatcta ttttatagta gattgaaact
     7441 agaatagtac acaactgctt ctaaaacatt gttagaaatc gatttgactg tcctgatcga
     7501 tttgtcctgc tcttatttca tttcactata ctttatatta taattgactg gtcaactatt
     7561 gataagtcaa tggataggag gaagaaatga tagagattca agatttactg tatcaactcc
     7621 gcttgtctga gcaagcgagt acgcaattgt ttgaaaaaag gcttgggatt agtttgacac
     7681 ggtatcagat tttactgttt ttgctggagc attctccttg taaccaaatg gcggttcagg
     7741 agcgtttgaa aattgatcag gctgctttga cacggcattt caagattttg gaaacggaag
     7801 gtttggtgga gcgtcatcgt aatcctgaaa atcagcggga agtgttggta gaggctgtga
     7861 agtatgccaa ggagcagtta gtggtgaatc cccctctgca acatatcaag gttaaggaag
     7921 agatggaaag tatcttaaca gagtttgaga gaacagaact cagccgttta ttaaataaat
     7981 tggttttggg tattgaaaat atagaaattt aaggagaaat agatgtcaat tattacaatt
     8041 attttaacaa cgatcgttgc tttggagcat ttttacattt tttatttgga aagtattgcc
     8101 acgcaatcag atgcgactag tcgtgtattt aatatggaaa aggaagaatt ggctcatccg
     8161 tcagtaagtt cattgttcaa aaatcaaggg atttataagg ctctgctagg agtctttctc
     8221 tggtatggca tttatttctc acagaattta gaaattgtga ctatttttgt cttatttgtg
     8281 attggtgctg cgacttacgg ctctttaaca gcggataaaa aaattatttt gaaacaaggt
     8341 ggatcagcta ttttggcctt gattagtatt ttactcttta aatacacttg aaggtcgatt
     8401 ctaatctcgc taatcctttt taatccagaa taagggaaat atgttatact tgtttttaag
     8461 aaaaaagtct cattgaattg gttttgagga gttagaaatg aaagtattag tgacaggttt
     8521 tgagcccttt ggaggggaaa agggcaatcc agctttggag gccattaaag gtttaccagc
     8581 tgaaatccat ggtgctgagg tccgttggct agaggtgccg acagtttttc acaaatctgc
     8641 tcaagtattg gaagaagaga tgaatcgtta tcaacctgac tttgtccttt gtattgggca
     8701 agctggtgga agaactagtt tgacacctga acgagtggcc attaatcaag acgatgcacg
     8761 tacttctgat aacgaagata atcaaccgat tgaccgtccc attcgcccag atggtgcttc
     8821 ggcctacttt agtagtttgc cgattaaagc gatggttcaa gctataaaaa aagagggctt
     8881 accggcctct gtttccaata cggcagggac ttttgtctgc agccatttga tgtatcaggc
     8941 tctctatttg gtagaaaaga aattttcata tgttaaggca ggttttatgc atattcctta
     9001 tatgatggaa caggtggtga acagaccgac tactccagct atgagtttag tggatattcg
     9061 gcgagggata gaagcagcaa tcggcgctat gatagaacat ggagatcagg aactcaagtt
     9121 ggtaggcgga gaaactcatt gatagaaaaa agcttgaggg gaaaaacctt caagcttttg
     9181 gacgttttcg agccaatact gctcggtaaa acataatttt agtgcattgg atataaggta
     9241 ggagtgaaaa actagcaatg ccaaaggtaa tccaattgag gaagtaccaa ggaagaagct
     9301 gtaaatctag gacaaagcgc tggaacttgt agcccttcat aaaggaacgg ctagttttta
     9361 ggattcgtct tggtgggacc tgtcctaggt ctagactata acagagaaga aattccacct
     9421 gtgaataggc ataatactgt ggaatataga ggatatttcc tacaatgatc aagatgagac
     9481 ctgcaagaaa gtagagtcca aagaccatga ggaaacgctc ggtttcaact gatgagagat
     9541 ctagatttgg aaactcagga tgtagggtga cgaatttttt ggctaaaaag ctactataaa
     9601 agaggaggta aatcccaagt aaattaggga tactccataa aaagagatag aaacgtttga
     9661 gaagtagggt caaaaaggtt tgagaaaagc gctcctcatc aaagagagct aggctgtttt
     9721 ttacagatgg ctccgtttta gaatctttca tgagtgtcag tgttgcatag acggaactgg
     9781 tcaaaagaat agtcccaata aaggagacta gtagaggaaa gaggtaggct tgaagtattt
     9841 ggccaagtat gctgaaaaat ggctgttcta aaacagtccc gtggatccga gataagggat
     9901 taagaaaact agataagatg accagcatac tgggaaggat atagaggaga aagagacggg
     9961 gggtgtcagc ctgaaaatgt tttgactcct gacgaattgt ttttaaatca atttttggat
    10021 agttcattct cttattatac catagttctt atacatagtt cgtgacagtt cctacttttt
    10081 ttgataaaat catacagtgt gtccttgggc acactgtatg aactgggact gtctttccca
    10141 gcttcggagg taaaaaatgt cagattcacc aatcaaatat cgtttgatta agaaagaaaa
    10201 acacacagga gctcgtctag gagaaatcat cactccccac ggtacctttc cgacacctat
    10261 gtttatgcca gttgggacac aagccactgt caaaactcag tcacctgaag aattgaagga
    10321 gatgggttcg ggaattatcc tatcaaacac ctatcatctc tggcttcgcc ctggagatga
    10381 actcattgca cgcgctggtg gtctccacaa gttcatgaat tgggaccagc ctatcttgac
    10441 agatagtggt ggttttcagg tttattcttt agcagatagc cgtaatatca cagaagaagg
    10501 agtaaccttt aaaaatcatc taaatggttc taagatgttc ctatccccag aaaaagccat
    10561 ctctattcag aataatctgg gttcagacat catgatgtcc tttgatgaat gtcctcagtt
    10621 ttatcaacct tatgactacg ttaagaaatc gatcgagcgt accagccgtt gggctgagcg
    10681 tggtttgaag gctcaccgtc gtccacatga ccaaggtttg tttggaattg tgcaaggtgc
    10741 aggatttgaa gaccttcgcc gccaatcagc tcatgatctt gtcagcatgg atttctcagg
    10801 ctactctatc ggtggtttgg cagtgggaga aacccatgaa gagatgaatg cggtcttgga
    10861 ctttacaact caactgctac ctgaaaataa acctcgttat ctgatgggtg tgggagcgcc
    10921 agatagcttg atcgatgggg tcattcgtgg ggtggatatg tttgactgtg tcttaccgac
    10981 tcgaattgct cgtaacggga cttgtatgac cagtcaagga cgtttggttg tgaaaaatgc
    11041 ccagtttgct gaggacttta cgccactgga tcctgagtgt gattgctaca catgtaataa
    11101 ctatacacgc gcttaccttc gtcacctgct caaggctgat gaaacctttg gtatccgctt
    11161 gactagctac cacaatcttt acttcttgct taacctgatg aagcaagtgc gacaagccat
    11221 catggatgac aatctcttgg aattccgtga gtattttgtg gaaaaatatg gctataataa
    11281 gtcaggacgt aatttctaaa atggaattga tataaaaaaa tcctaagttt tctcttagga
    11341 tttttcttct ttttttgata gaataaagtg tacaatgaaa ggaagaataa actcgtatgc
    11401 gcattaaatg gttttccttg attaggatta caggtttact tttggtactc ttgtatcact
    11461 tctttcagac gatctttcct ggaggatttt tcggggtaga tgtctttttc acattttcag
    11521 gtttcctgat tacagctcta ctcattgaag aattttctaa aaaccatgag attgatttga
    11581 taggattttt tagaagacgc ttttatcgga ttgtgccacc tgtggttttg atggtcttgg
    11641 tgaccatgcc ttttactttc ttggttcgcc aagactatgt tgctggaatt ggtggccaga
    11701 ttgcgagcgt cttaggcttt atgaccaact tctatgaact tctaacaggt gggagttatg
    11761 aatctcagtt cattcctcat ttgtttgttc ataattggag tttggcagtt gaggttcact
    11821 actatattct ttggggattg gcagtttggt tcttatccaa acaagctaaa tcaaatggtc
    11881 agttgaaggg gatggtcttt ctcttatctg ctgttgcctt cttgatcagt ttcttctcca
    11941 tgtttattgg tagttttcta gtgacctctt attcctctgt ttatttctcc agtttaactc
    12001 atgtctatcc attctttttg ggaagtatgt tagcaactat tgtaggcgtt cgtcagacga
    12061 cttccctcgt caagcagttg gataaaatct gggatttacg aaagactttg gtagtttttg
    12121 gaggaggctt tggtttccta gttcttttga ctttctttgt caaattcact tatctttttg
    12181 cctatcttat cggcttcttg cttgccagtc ttgcagctct tgccatgatt ctggcggcgc
    12241 gtgtcttaca tgaaaagaca catcatatac aggagccgaa gattatcagc tttttagcgg
    12301 atactagcta tgcggtttat cttttccatt ggcctttcta tatcattttc tcacagttga
    12361 catcaaatct tcttgctgta ttactgactc tgatttgttc ttatggcttt gccagtctgt
    12421 cattttatgt attggaacct tggattgcag gcaagaacac acctattgtc caaacccttc
    12481 gtcccctgcc ttatattcac gcaattcttg cagcaggtac aggaatcttg accatcattg
    12541 tctgcacggt gaccttgttg gcaccacaag tgggagcgtt tgagacagac ttgactgtca
    12601 atggcttgaa gcaagctgca acaaatattg gccagaccaa ggtgatggca gaacgggcag
    12661 atgcaaacag tttgggaatt gctgatggca ctatgttaat tggtgactca gtggctttaa
    12721 gggcaaatac agcactacag acagctcttc ctggagcaca gattaacgcg caggtcagcg
    12781 taacaaccaa gaccgcaaat gaaatcatgc taaataatag ccagaataaa tttttaccta
    12841 agacggtggt cattgcgact ggggtaaata atcctgagaa ttacaaggat gactgggaca
    12901 gtatcgtgaa aaatcttcct aagggacacc atatgatttt ggtgactcct tatgagggag
    12961 ataagacaaa agagacctat gccatcgttg agaaggctgc tgcctatatg agagaattgg
    13021 cagagaagac accttacatt acgatagcag attggaatca agttgcgaaa gagcatccag
    13081 aaatttgggc aggaacagac caggttcatt tcgggagtga gagtagcact atcgaagcag
    13141 gagcaaaatt gtatgcagat acgattgcca cagctttgca gacagctcaa gacaagccgg
    13201 ttaaatcaaa ataacttgta ttaaaaaaga gaagagttgg agaaatccag ctcttttaaa
    13261 atatctccaa aaaataggtc tataccattt acaaataaaa aagaaaggtt tataatgtaa
    13321 ttgacataat aaattgtaga atcaatcttt taaggaggtt aacattatgc ctaactatat
    13381 taaagcggat cagtttttct acccacacgg agttcgtcga ggtggttact tggaacttgt
    13441 ggacggcaag tttgggaaac atgtagagca gattcctgaa ggggctgagg tgattgacta
    13501 tacaggttat agcattgccc caggtcttgt ggatactcat attcatggat atgcaggtgt
    13561 agatgtgatg gacaacaaca ttgaaggtac attgcatact atgagtgaag gacttcttag
    13621 taccggtgtt accagtttct tacccacaac tttaacagcc acttatgagc aattgcttgc
    13681 agtcactgaa aatcttggaa accattataa agaagcaaca ggtgctaaga ttcgtgggat
    13741 ttattatgaa ggtccatatt tcacagaaac ttttaagggg gcacaaaatc caacttatat
    13801 gagagacccg ggtgttgagg agtttcattc ttggcaaaaa gcggcaaatg gcttgcttaa
    13861 taaaattgcc cttgcaccag aacgtgatgg ggtggaagac tttgttcgta cagttacggg
    13921 cgaaggtgtg acggttgctc ttggacattc aaacgcgact tttgatgaag ccaaaaaagc
    13981 agtcgatgct ggagcgagtg tttgggtgca tgcctacaat ggaatgcgtg ggttgactca
    14041 ccgtgaattg ggtatggttg gagccatgta ccaattgcca catacctatg cagagttgat
    14101 ctgtgatggt caccacgtag atccaaaggc ttgcgaaatt cttatcaaac aaaaaggaac
    14161 agaaaacatc gctcttatca cagactgtat gacagctggg ggattggaag acggagatta
    14221 tatgttggga gaattcccag ttgtcgttgc aaatggaact gcacgcctca aatcgacagg
    14281 taacttggca ggttctatcc tcaaactcaa agatggtttg aagaatgtgg tcgaatgggg
    14341 aattgcgaat ccgcatgaag cagtcatgat ggccagcttc aacccagcta aatccgttca
    14401 catcgatgac gtctgtggcc aaatccgtga aggctacgac gctgacttca tcgtattaga
    14461 taaagatttg gaattggtag caacctacct agatggcgta aaacgttatc aagcataaga
    14521 gagaaagcag aagttagaga ctaacttctg cttttttatg cagggtactt tactggtaaa
    14581 taaaaagtta aaaaaatcac aaaaaaagct tgaagaaaca aagaaaaagg cttacaattg
    14641 aactataaat attacaaata aaaaaacgga ggagtgcttt atgaaagcct atacttatgt
    14701 taaaccagga cttgcttctt ttgttgatgt agacaaacca gttattcgta agccaacaga
    14761 cgctattgtg cgtattgtaa aaaccactat ttgtggaaca gacctccata ttatcaaagg
    14821 ggatgttcct acttgccaaa gtggtaccat tcttggccac gaaggaattg ggattgttga
    14881 agaagttggg gaaggagttt ccaacttcaa aaaaggtgac aaggtcttga tttcttgcgt
    14941 ctgtgcctgt ggtaaatgct actactgtaa aaaaggaatt tatgcccact gtgaagacga
    15001 agggggctgg attttcggtc acttgattga tggtatgcag gctgaatatc tacgtgtccc
    15061 tcatgcagat aatactcttt accatactcc agaagacttg tcagatgaag ctttggttat
    15121 gctgtcagac attctgccta ctggatatga aattggtgtc ttaaaaggga aagtagaacc
    15181 tggttgcagc gtagccatta ttggttcagg tccagttgga ttggctgctc ttttaacagc
    15241 ccaattctat tcaccagcta aattgattat ggtggacttg gatgataacc gtttggatac
    15301 tgcagtatcc tttggtgcta ctcataaggt aaactcttca gaccctgaaa aagcgataaa
    15361 agaaatttat gatttgacag atggtcgcgg tgtagatgtc gctattgaag ctgtagggat
    15421 tcctgaaaca tttgatttct gtcaaaagat tatcggtgta gacggaacgg ttgccaactg
    15481 tggtgtgcat ggtaaaccag ttgaattcga tttagataaa ctttggattc gcaacatcaa
    15541 tgtaacaact ggtttggtat ctacaaatac gactccacaa ttgttgaaag cacttgaaag
    15601 tcataagatt gaaccagaaa aattggtaac tcactatttc aaactcagtg aaattgaaaa
    15661 agcctatgaa gtcttcagta aggcagcaga ccaccatgcc attaaggtca ttatcgaaaa
    15721 cgatatctca gaagcctaag tagtaaaaat atttttgtac ataagtgaat agaaattcag
    15781 tcatccatca gatggctgga ttttttatca aaaaattaag aaatgagcat atttctttcc
    15841 ttgtctggcg gaattggtta taatatacgg tacaaaggaa tcaatgaata tgtatcgtgt
    15901 tatagaaatg tacggagatt ttgaaccgtg gtggttctta gaaggttggg aagaagatat
    15961 tgtagcaagt agaaaatttg accagtatta tgatgctctc aaatactaca aaacttgctg
    16021 gtttagattg gaacaagaat cccctcttta taaaagtaga agcgacttga tgaccatttt
    16081 ttgggacccg gaagaccaac gctggtgtga tgaatgtgat gagtatttac aacaatacca
    16141 ttctttggct cttttgcagg atgagcaggt tatcccagac gaaaaactac gctcaggcta
    16201 tgaaaaacaa accagtcagg aaaggaatcg ttcttgccgt atgaaattaa aatagagaaa
    16261 agtaactttt ttggagttgc tttttttatt tttctaacgc tttgcgaata gtataggtga
    16321 ggaggtaagt atggttcaag aaattgcaca agaaatcatt cgttcagctc ggaaaaaagg
    16381 gacgcaggat atttattttg tccctaagtt agacgcctat gagcttcata tgagggtagg
    16441 agacgagcgc tgtaaaattg gtagctatga ttttgaaaag tttgcagccg ttatcagtca
    16501 ctttaagttt gtggcgggta tgaatgtggg agaaaaaaga cgtagtcaac tgggttcctg
    16561 tgattatgcc tatgaccaga agatggcgtc tctacgttta tctactgtag gcgattatcg
    16621 gggacatgag agtttggtta tccgtttgtt gcacgatgag gagcaggact tgcatttttg
    16681 gtttcaggat attgaagaat taggcaagca gtacaggcaa cggggactct atctttttgc
    16741 tggcccggtt gggagtggta agacgacctt gatgcatgaa ttgtccaagt cactctttaa
    16801 aggacagcaa gttatgtcca tcgaagatcc tgtcgaaatc aagcaggacg acatgcttca
    16861 gttgcagttg aacgaagcaa tcggcctaac ctatgaaaat ctaatcaaac tttccttgcg
    16921 tcatcgacca gatctcttga ttatcggaga aattcgtgac agcgagacgg cgcgtgcagt
    16981 ggtcagagct agtttgacag gtgcgacagt cttttcaacc attcacgcca agagtatccg
    17041 aggtgtttat gagcgtctgc tggagttggg tgtgagtgaa gaagaattgg cagttgttct
    17101 gcaaggagtc tgctaccaga gattaatcgg gggaggagga atcgttgact ttgcaaacag
    17161 agattatcaa gaacaccaag cagccaagtg gaatgagcaa attgaccagc ttcttaaaga
    17221 tggacatatc acaagtcttc aggctgagac ggaaaaaatt agctacagct aagcaaaaaa
    17281 atatcatcac cctatttaac aatctctttt ctagcggttt tcatctggtg gagactatct
    17341 cctttttaga taggagtgct ttgttggaca agcagtgtgt gatccagatg cgtgcgggct
    17401 tgtctcaagg gaaatcattc tcagaaatga tggaaagttt gggattttca agcaccatta
    17461 tcactcagtt atccctagct gaagttcatg gaaatctcca cctgagtttg ggaaagatag
    17521 aagaatatct ggacaatctg gctaaggtca agaaaaaatt aattgaagta gcgacctatc
    17581 ctttgatttt gctgggtttt cttctcttaa ttatgctggg gctacggaat tacctgctcc
    17641 cacaactgga tagtagcaat attgccaccc aaattatcgg taatctgccc caaatttttc
    17701 taggcatggt agggcttgtt tccgtgcttg cccttttagc actaactttt tataaaagaa
    17761 gttctaagat gagtgtcttt tctatcttag cacgccttcc ctttattgga atctttgtgc
    17821 agacctactt gacagcctat tatgcacgtg aatgggggaa tatgatttca cagggaatgg
    17881 agctgacgca gatttttcaa atgatgcagg aacaaggttc ccagctcttt aaagaaatcg
    17941 gtcaagatct ggctcaaacc ctgaaaaatg gccgtgaatt ttctcagacg ataggaacct
    18001 atcctttctt taggaaggaa ttgagtctca tcatagagta tggggaagtt aagtccaagc
    18061 tgggtagtga gttggaaatc tatgctgaaa aaacttggga agcctttttt acccgagtca
    18121 accgcaccat gaatttggtg cagccactgg tttttatctt tgtggcactg attatcgttt
    18181 tactttatgc ggcaatgctc atgcccatgt atcaaaatat ggaggtaaat ttttaaaatg
    18241 aaaaaaatga tgacattctt gaaaaaagct aaggttaaag cttttacatt ggtggaaatg
    18301 ttggtggtct tgctgattat cagcgtgctt ttcttgctct ttgtacctaa tctgaccaag
    18361 caaaaagaag cagtcaatga caaaggaaaa gcagctgttg ttaaggtggt ggaaagccag
    18421 gcagaacttt atagcttgga aaagaatgaa gatgctagcc taagcaagtt aaaagacgat
    18481 gggcgaatca cggaagaaca ggctaaagct tataaagaat accatgataa aaatggagaa
    18541 gcaaatcgta aagtcaatga ttaaggcctt taccatgctg gaaagtctct tggttttggg
    18601 tcttgtgagt atccttgcct tgggcttgtc cggctctgtt cagtccactt ttgcggcggt
    18661 agaggaacag attttcttta tggagtttga agaactctat cgggaaaccc aaaaacgcag
    18721 tgtagccagt cagcaaaaga ctagtctgaa cttagatggg cagacgctta gcaatggcag
    18781 tcaaaagttg acagttccta aaggaattca ggcaccatca ggccaaagta ttacatttga
    18841 ccgagctggg ggcaattcgt ccctggctaa ggttgaattt cagaccagta aaggagcgat
    18901 tcgctatcaa ttatatctag gaaatggaaa aattaaacgc attaaggaaa caaaaaatta
    18961 gggcagtgat tttactggaa gcagtagtcg ctctagctat ctttgccagc attgcgaccc
    19021 tccttttggg acaaattcaa aaaaataggc aagaggaagc aaaaatcttg caaaaggaag
    19081 aagtcttgag ggtagctaag atggccctgc agacggggca aaatcaggta agcatcaacg
    19141 gagttgagat tcaggtattt tctagtgaaa aaggattgga ggtctaccat ggttcagaac
    19201 agttgttggc aatcaaagag ccataaggtc aaggctttta ccttgttaga atccctactt
    19261 gccctcattg tcatcagtgg gggattactc ctttttcaaa ctatgagtca gctcctcatt
    19321 tcagaagttc gctaccagca acaaagcgag caaaaggagt ggctcttgtt tgtggaccaa
    19381 cttgaggcag aattagaccg ttcgcagttc gaaaaagtag aaggcaatcg cctatacatg
    19441 aagcaagatg gcaaggacat cgccatcggt aagtcaaagt cagacgattt tcgtaaaacc
    19501 gatgctagtg gacgggggta tcagcctatg gtttatggcc tcaaatcagc tcagattaca
    19561 gaggacaatc aactggttca ttttcgtttc cagtttcaaa aaggcttaga aagggagttc
    19621 atctatcgtg tggaaaaaga aaaaagttaa ggcaggtgtt ctcctctacg cagtcaccat
    19681 agcagccatc tttagtcttt tgttgcaatt ttacttgaac cgacaagtcg cccactatca
    19741 agactatgct ttgaataaag aaaaattggt tgcttttgca atggctaaac gaaccaagga
    19801 taaagccgag caagaaagtg gggaacagga ctttaatcta ggtcaggtaa gctatcaaaa
    19861 caagaaaaca agcttggtga cgactgttcg tacgtctaag agccaatatg agtttctgtt
    19921 tccttcagtc aaaatcaaag aagagaaaag agataaaaag gaagagatag cgaccgattc
    19981 aagcgaaaaa gtggagaaga aaaaatcaga agagaagcct gaaaagaaag agaattccta
    20041 gtcaattcaa ctataatgcg ttgaatccag aatagtccac tatagtttct ataaaattgc
    20101 tggaaatgga tattaagctc caattcattt gtttatatct tatttcagtt tactatactt
    20161 tgtgctaaac taaagatatg aaacatgatt ttaaccacaa agcagaaact ttcgattccc
    20221 ctaaaaatat cttcctcgca aacttggtat gtcaagcagc cgagaaacag attgatcttc
    20281 tatcagacaa agaaatttta gatttcggtg gtggcacggg tctattagcc ttgcccctag
    20341 cccctagcca agcaggctaa gtcagtcact cttgtagaca tttctgagaa aatgttggag
    20401 caagctcgtt tgaaagtgga gcagcaagca atcaagaata tccagttttt ggagcaagat
    20461 ttaccgaaaa atcccttgga gaaagagttt gattgccttg ctgttagtcg ggttcttcat
    20521 catatgcctg atttggatgc ggctctctca ctgtttcatc aacatttgaa ggaagatggg
    20581 aaactcatca ttgctgattt taccaagaca gaagctaatc atcatggatt tgatttagct
    20641 gaactggaaa acaagctaat tgagcatggt ttttcatctg tgcatagtca gattctctat
    20701 agcgctgaag acctgtttca aggaaatcac tcagaattct ttttaatagt agcccaaaaa
    20761 tcactcgcct agtcagggag tgatttttct ataaggatgg aaaaaagaag ggaaatttga
    20821 taagatagga atatggattt tgaaaaaatt gaacaagctt atacctattt actagagaat
    20881 gtccaagtca tccaaagtga tttggcgacc aacttttatg acgccttggt ggagcaaaac
    20941 agcatctatc tggatggtga aactgagcta aaccaggtca aagacaacaa tcaggccctt
    21001 aagcgtttag cactacgcaa agaagaatgg ctcaagacct accagtttct cttgatgaag
    21061 gctgggcaaa cagaaccctt gcaggccaat caccagttta caccggatgc tattgctttg
    21121 cttttggtgt ttattgtgga agagttgttt aaagaggagg aaattactat cctcgaaatg
    21181 ggttctggga tgggaattct aggcgctact ttcttgacct cgcttgataa aaaggtggat
    21241 tacttgggaa tggaagtgga tgatttactg attgatctgg cagctagcat ggcagatgta
    21301 attggtttgc aggctggctt tgtccaagga gatgccgttc gtccacaaat gctcaaagaa
    21361 agcgatgtgg tcatcagtga cttgcctgtt ggctattatc ctgatgatgc cgttgcgtcg
    21421 cgccatcaag ttgcttctag ccaagaacat acttacgccc atcacttgct catggaacaa
    21481 gggcttaagt acctcaagtc agacggatac gctatttttc tagctccgag tgatttgttg
    21541 accagtcctc aaagtgattt gttaaaagaa tggctgaaag aagaggcgag tctggttgct
    21601 atgattagtc tgcctgaaaa cctctttgct aatgccaaac aatctaagac tatttttatc
    21661 ttacagaaga aaaatgaaat agcagtagag ccttttgttt atccacttgc tagcttgcaa
    21721 gatgcaagtg ttttaatgaa atttaaagaa aattttcaaa aatggactca aggtactgaa
    21781 atataaaata gattttgtta taatagttga aaacgcttaa aaaggggtat catgttatga
    21841 caaaaacaat tgcaatcaat gcaggaagtt caagtttgaa atggcaatta tacttaatgc
    21901 cagaagaaaa agtattggcg aaaggtttga ttgaacgtat cggtttgaaa gattcaattt
    21961 caactgtaaa atttgacggc tgttctgaac aacaaatttt ggatattgaa aatcatacac
    22021 aagccgttaa aattttattg gatgacttga ttcgtttcga tattatcaag gcttatgacg
    22081 agattacagg tgttggacat cgtgttgttg ctggtggaga atatttcaaa gaatcaacag
    22141 ttgttgaggg agatgtttta gaaaaagttg aagagttgag tttgttggct cctctacaca
    22201 acccggccaa tgcagcaggt gttcgtgcct tcaaggaatt gttgccagac attaccagtg
    22261 tagttgtttt tgatacttcc ttccacacaa gtatgccaga gaaagcttat cgctaccctc
    22321 taccaacaaa atattacaca gaaaacaagg ttcgtaaata cggtgctcat ggtacaagtc
    22381 accagtttgt agcaggagag gctgcaaaac tcttgggacg tccattagaa gacttgaagt
    22441 taattacctg tcatattggt aacggaggct caattacagc tgtgaaagcc ggcaaatctg
    22501 tagacacttc tatggggttc actcctcttg gtggtattat gatgggaacg cgtacagggg
    22561 atattgatcc agctatcatt ccttatttaa tgcaatatac agaggatttt aacacaccag
    22621 aagatatcag tcgtgttctt aaccgtgaat caggtctttt gggagtttct gctaattcta
    22681 gcgatatgcg cgatatagaa gcagctgtag cagaagggaa tcacgaggct agcttggctt
    22741 atgaaatgta tgttgaccgt atccaaaaat atatcggtca gtaccttgca gtgctaaatg
    22801 gagcagatgc cattgttttc acagcaggtg tcggtgaaaa tgcagagaat ttccgtcgtg
    22861 atgtaatctc agggatttcg tggtttggtt gtgatgttga tgatgaaaag aatgtctttg
    22921 gcgttacagg agacatctca acagaggcag ctaaaatccg tgtcttggtt attccaacag
    22981 atgaagaatt agtcattgcc cgtgacgttg aacgcttgaa aaaataagtg aaactaaaaa
    23041 aatattcaat acaaggagtt gggaaagtta tttttccagc ttctttttct gatgaaattg
    23101 tccaaaacct tgctatgatt ggcttttttg aaaaatatgg tataatagta gtaatttaat
    23161 agatggagtt gagttttgaa gaaaaacttt cgtgtaaaaa gagagaaaga ttttaaggcg
    23221 attttcaagg aggggacaag ttttgctaat cgcaaatttg tggtctacca attagaaaac
    23281 cagaaaaacc attttcgagt aggtctatca gttagcaaaa aactggggaa tgccgtcact
    23341 agaaatcaaa ttaagcgacg gattcggcat attatccaga atgcaaaagg gagtctggta
    23401 gaagatgtcg actttgttgt cattgctcga aaaggagtcg aaaccttggg atacgcagag
    23461 atggagaaaa atctactcca tgtattaaaa ttatcaaaga tttaccggga aggaaatggg
    23521 agtgaaaaag aaactaaagt tgactagttt gctaggactg tctctgttaa tcatgacagc
    23581 ctgtgcgact aatggggtaa ctagcgatat tacagccgaa tcggctgatt tttggagtaa
    23641 attggtttac ttctttgcgg aaatcattcg ctttttatcg tttgatatta gtatcggagt
    23701 ggggattatt ctctttacgg tcttgattcg tacagtcctc ttgccagtct ttcaggtgca
    23761 aatggtggct tctaggaaaa tgcaggaagc tcagccacgc attaaggcgc ttcgagaaca
    23821 atatccaggt cgagatatgg aaagcagaac caaactagag caggaaatgc gtaaagtatt
    23881 taaagaaatg ggtgtcagac agtcagactc tctttggccg attttgattc agatgccggt
    23941 tattttggcc ttgttccaag ccctatcaag agttgacttt ttaaagacag gtcatttctt
    24001 atggattaac cttggtagtg tggatacaac ccttgttctt ccgattttag cagcagtatt
    24061 caccttttta agtacttggt tgtccaacaa agctttgtct gagcgaaatg gcgctacgac
    24121 tgcgatgatg tatgggattc cagtcttgat ttttatcttt gcagtttatg cgccaggtgg
    24181 agtcgcccta tactggacag tgtctaatgc ttatcaagtc ttgcaaacct atttcttgaa
    24241 taatccattc aagattatcg cagagcgcga ggccgtagta caggcacaaa aagatttgga
    24301 aaatagaaaa agaaaagcca agaaaaaggc tcagaaaacg aaataaataa ggaggaatct
    24361 ggtaatggta gtatttacag gttcaactgt tgaagaagca atccagaaag gattgaaaga
    24421 attagatatt ccaagaatga aggctcatat caaagtcatt tctagggaga aaaaaggctt
    24481 tcttggtcta tttggtaaaa aaccagccca agtggatatt gaagcgatta gtgaaacgac
    24541 tgttgtcaaa gcaaatcaac aggtagtaaa aggcgttccg aaaaaaatca atgatttgaa
    24601 cgaacctgtg aagacggtta gtgaagaaac cgttgacctt ggtcatgtgg ttgatgctat
    24661 taaaaaaata gaggaagaag gtcaaggtat ttctgatgaa gtcaaggctg aaatcttaaa
    24721 acatgaaaga catgccagca ctatcttaga agaaactggt cacattgaga ttttaaatga
    24781 acttcaaatc gaggaagcga tgagggaaga agcaggcgct gatgaccttg aaactgagca
    24841 agaccaagct gaaagtcaag aactagaaga cttgggcttg aaagttgaaa cgaactttga
    24901 tattgaacaa gtagctacgg aagtaatggc ttatgttcaa acgattattg atgacatgga
    24961 tgttgaggct acactttcaa atgattataa ccgtcgtagc atcaatctac aaattgacac
    25021 caacgaacca ggtcgtatta tcggctacca tggtaaagtc ttgaaggcct tgcaactgtt
    25081 ggctcaaaat tatctttaca accgctattc cagaaccttc tacgttacaa tcaatgtcaa
    25141 tgattatgtc gaacaccgtg cagaagtctt gcagacctat gcgcaaaaat tggcgactcg
    25201 tgttttggaa gaagggcgca atcataaaac agatccaatg tcaaatagcg aacgcaagat
    25261 tatccatcgt attatttcac gtatggatgg cgtgactagt tactctgaag gtgatgagcc
    25321 aaatcgctat gttgttgtag atacagaata agtaaaatca ggtttatcct gattttttgc
    25381 tagttagagg aggttaaact gatgttgaat aagataagag actatttaga ctttgctggt
    25441 ttgcagtacc gtaatcctga taaagcggga gcagagcgag agaagatgct ggcattccgc
    25501 cacaaaggac aagaagctcg aaaggctttt acagaactgg ccaaagcctt tcaagcaagc
    25561 catccagaat ggcaactcca acagactagt cagtggatga atcaggccca gcgtttgaga
    25621 ccacattttt gggtttatct acagagagac ggacaagtga cagaacctat gatagccttg
    25681 cgtttgtatg gaaatccttc tgattttggt atttccttag aagtcagttt catcgaacgt
    25741 aagaaggatg agcaaacact gggcaagcag gccaaagttt tagaaattcc aaccgttaaa
    25801 gggatttatt atctaaccta ctctaatggt caaagtcaac ggtgggaggc gaatgaagaa
    25861 aagcgtcgta ctttacgcga gaaggtgaga agtcaagaag ttcgaaaagt tttagtgaag
    25921 gtagatgttc ctatgacaga aaattcgtct gaagaagaaa tcgtagaagg cttattgaag
    25981 tcttattcta aaattcttcc ctattatcta gctacgagaa aataagataa tttgtaaaac
    26041 atcataaatc atacagtcca agagtgaaca gtccgctgtg taattcttgg tctttttgtt
    26101 tgcgctttcg cattatataa taaacttaca aaaacaattc aaaaggagaa caattatgga
    26161 agtcgtttca agtgttctaa attggttttc tagcaatatt ttgcagaatc ccgcattttt
    26221 cgtaggttta ttggtgttga taggatatgc acttttgaaa aaacctgccc atgacgtttt
    26281 ttcagggttt gttaaagcaa cagtagggta tatgttgctt aacgtgggtg ctggtggttt
    26341 ggttacaacc tttcgtccaa tcttagcagc tcttaactac aaattccaaa ttggtgcagc
    26401 ggttatcgac ccttattttg gacttgctgc agcaaacaac aaaattgtag cagagtttcc
    26461 agattttgtt ggaactgcaa ctacagctct attgattggt tttggaataa atatcttgct
    26521 cgtagctctt cgaaagatta cgaaggtaag aaccctcttt attactggtc acatcatggt
    26581 acaacaagct gcaacagtat ctcttatggt tctattctta gtaccacaat tgcgcaatgc
    26641 ttacggtaca gcagcgattg gtatcatctg tggactttac tgggcagtta gttcaaatat
    26701 gactgttgag gcaactcaac gcttgactgg tggtggcgga tttgcgattg gtcaccaaca
    26761 gcaatttgca atctggtttg tagataaagt agcaggacgc tttggtaaga aagaagaaag
    26821 tttagacaat cttaaattac ctaagttcct ctcaatcttc cacgatacag ttgttgcatc
    26881 tgctaccttg atgctcgtat tcttcggagc cattctttta atcttgggtc cagacattat
    26941 gtctaataaa gaagtcatca cttcaggaac tctattcaat cctgctaaac aagatttctt
    27001 tatgtacatt atccaaacag cctttacctt ctcagtttac ttgttcgttt tgatgcaagg
    27061 tgtccgaatg ttcgtatctg agttgacaaa cgccttccaa ggtatttcaa acaaattgtt
    27121 gccaggttca ttcccagcgg ttgacgttgc agcttcttat ggatttggtt ctccaaatgc
    27181 tgtcttgtca ggatttacct ttggtttgat tggtcaattg attacaattg ttttgctcat
    27241 cgtctttaaa aatccgattc ttattattac aggatttgta ccagtgttct ttgacaatgc
    27301 agccattgcg gtctacgctg ataaacgcgg cggatggaaa gcggctgtta tcctttcctt
    27361 tatatcgggt gtccttcaag ttgctctagg agctctttgt gtggcccttc tcgatttggc
    27421 atcttatggt ggctaccatg gaaatatcga ctttgaattc ccatggcttg gatttggata
    27481 tatcttcaaa taccttggta ttgttggtta tgtacttgtg tgtctcttct tgcttgttat
    27541 tcctcaactt caatttgcca aagcaaaaga taaagagaaa tattacaacg gtgaagttca
    27601 agaagaagct tagtatctag aaaaggagaa ataaaatggt taaagtatta gcagcgtgcg
    27661 gaaatggaat gggttcatca atggttatca agatgaaggt tgaaaatgct ctccgtaagc
    27721 ttaatcaaac agattttaca gtcaattcat gcagtgtcgg tgaagctaaa ggtttagcag
    27781 taggatatga catcgtaatc gcttctcttc atttgattca agaattggaa gggcgaacta
    27841 atgggaagtt aattgggctt gataacttga tggatgataa agaaatcacc gaaaaactca
    27901 gtcaagcact acagtaaaag gttggagggg gctggacaga aactgagagt tatcgtttct
    27961 gtccttctcc ctctttaaat aaaggaggca gatatgaatt taaaacaagc tttaattgac
    28021 aatgactcga tccgactagg tttagaggct aacaattgga aagaagcagt caaggtagca
    28081 gtagatccct taattgaaag tggggcaatt ttgccagagt attacgatgc tatcattgaa
    28141 tcgactgaag agtatgggcc ttactatatc ttgatgccag gtatggctat gccccacgct
    28201 agacctgaag ctggtgtgca aagtgatgcc ttttcattga ttaccttaca aaatcctgtt
    28261 gtattttcag atgggaaaga ggtatctgtt ttgttggcac tagcagcaac aagctcaaaa
    28321 attcacacaa gtgtagccat tccacaaatt attgccctgt ttgaattaga cgattctatt
    28381 gcacgtttac aggcttgcca gactaaagaa gatgtcttgg ctatgattga agaatctaag
    28441 gatagccctt atctcgaagg attggacttg gaaagttaga aagaggaata aagaaatgac
    28501 aaaaagaata cctaatttac aagttgcatt agaccattca gacttgcaag gagcgattaa
    28561 agcagctgtt tctgttggtc aggaagtaga tattatcgaa gctggaactg tttgcttgct
    28621 tcaagttgga agtgaactgg ctgaagtctt gcgtagcctt ttcccagata agattattgt
    28681 ggcagacaca aaatgtgctg atgctggtgg aacagttgct aaaaataatg cggttcgtgg
    28741 agcagactgg atgacttgta tctgttgtgc aaccatccct actatggaag cagctctaaa
    28801 ggctatcaag actgaacgag gagaacgagg cgaaatccag atcgagcttt atggcgattg
    28861 gacttttgaa caagctcagc tttggctaga tgcaggtatc tcacaagcta tttatcacca
    28921 atctcgtgat gctcttcttg ctggtgaaac ttggggtgaa aaagacctta ataaggttaa
    28981 aaaactcatt gacatgggct tccgtgtatc tgtaacaggt ggtctagatg tagatactct
    29041 caaactcttt gaaggtgttg atatctttac ctttatcgca ggtcgtggaa ttacagaggc
    29101 tgcggatcca gcaggagcag cgcgtgcctt caaggatgaa atcaaacgaa tttgggggta
    29161 aatcatggta cgtccaattg gaatttatga aaaggcaacc ccaacacact ttacttggct
    29221 agaacgttta aattttgcca aggagttagg ctttgatttt atcgagatgt ctattgacga
    29281 acgtgacgag cgtttagcaa gacttgactg gagtaaggaa gaacgcttgg aagttgtcaa
    29341 agaaatctat gaaactggtg ttcgtattcc ttctatctgt ttttcaggcc atcgtcgcta
    29401 cccattggga tcaaaagatt cagttctaga ggaaaaatct ctagaactca tgaaaaaatg
    29461 tatcgaatta gctcaagact tgggagttcg tacgattcaa ttagctggtt acgatgttta
    29521 ctatgaggaa aagtcacccc agacacgcca acgttttatc aaaaatttga gaaaagcctg
    29581 tgactgggct gaagaagctc aggtggtact tgctattgaa attatggatg atcctttcat
    29641 cagtagcatc gaaaaatatt tggctataga aaaagagatt gactctccct tcctctttgt
    29701 atatccagat attggtaatg tgtctgcatg gcataatgat atctatagtg agttttatct
    29761 tggtcatcat gccatcgcag ctctccatat caaggatact tatgcagtga cagaaagttc
    29821 aaagggccag ttccgagatg tacctttcgg gcaaggttgt gtcaaatggg aagaagcttt
    29881 cgatatttta aaggaaacca attataatgg acctttccta atcgaaatgt ggtctgaaaa
    29941 ttgtgaaaca gtagaagaaa cacgcgcagc cattcaagag gcgcaagctt ttctctatcc
    30001 actcattaag aaagcaggtt tgatgtaaga tgaatcaagt aatcaatgct atgcgtaaac
    30061 gagtctgtga tgccaatcaa tcattgccaa aacatggact tgtcaaattt acctggggga
    30121 atgtatctga agtcaatcgc gaactcggtg tcattgttat caaaccatca ggcgtggatt
    30181 atgacgaatt gacacctgaa aacatggtag tgactgatct agatggtaag atcctagaag
    30241 gggatttaag accatcttcc gacctcccaa ctcatgtgca attatataag gcttggtcag
    30301 aaattggtag tgtggttcac acccattcga cagaagctgt tggttgggct caggcaggtc
    30361 gtgatattcc tttctacgga acaacccatg cagattattt ctacggttca atcccttgcg
    30421 cccgtagttt gaccaaggac gaagtagaag tgacctatga aaaagatact ggcctggtta
    30481 tcgtagaaga gtttgaacat cgcggactta acccggttga agtaccagga attgttgtac
    30541 gcaatcacgg tccattcacc tggggcaaaa atccagagaa tgctgtttat cactctgtcg
    30601 tactagagga agtatcaaag atgaatcgct ttacagaaca aatcaatcca agagttgaac
    30661 ctgctcccca gtacatacta gaaaaacact accaacgtaa acatggacca aatgcttatt
    30721 atggtcaaaa gtaagaacga tgaaggagga gaaaaagata aatttagctc ctctttttac
    30781 atttgatttt tattgagagt aaagttggag ttgaagtaat tttgaaagat tttttagaaa
    30841 tagcgcttga tatatatatg gtaaaataaa aagaattgct gtgatatcaa tagatttggg
    30901 ggatttttta atatggtact ggataaggca agttgtgatt tacttcaata tttgatggat
    30961 caagaaacgt ccaaaacgat tatggcgatt tcgaaagatt tgaaagagtc aagaaggaaa
    31021 atttattatc acattgacaa aatcaatgct gctctgggtg acgaggcgct tcacatcatt
    31081 agtattccac gaattggtat tcacttaacg gaagagcaga gagatgcttg ttgtaaacta
    31141 ttatcggaag tagattcgta cgattatatc atgagtgcgc atgaacgtat gatgataatg
    31201 ttactatgta taggtatttc taaagaacgt attacgattg aaaaattgat agagttaaca
    31261 gaggtatcta ggaatactgt tctcaatgat ttgaatagta ttcgttatca actaactttg
    31321 gaacaatatc aggtgacctt gcaagtgagc aagtcacagg gataccacct tcatgcccac
    31381 cctcttaata aaattcagta tcttcaatcg cttctatatc atatttttat ggaagaaaat
    31441 gccacttttg tatctatttt agaagataag atgaaagaga ggttagatga tgagtgtttg
    31501 ctttctgttg aaatgaacca attttttaag gaacaggttc ctttagttga acaagattta
    31561 gggaagaaaa taaaccatca tgaaataact tttatgttgc aggttctacc ttatttgctg
    31621 ttaagctgtc ataatgttga acagtatcaa gaaagacatc aggatataga gaaagagttt
    31681 tctttgataa gaaaaagaat agagtatcag gtgtctaaga aattaggaga acggttgttt
    31741 caaaagtttg aaatttcttt gtcaggactt gaagtttctc ttgtagctgt tctcctcctc
    31801 tcctatcgta aagatttgga tattcatgca gaaagtgatg attttcggca attaaaactt
    31861 gctttagaag aatttatctg gtattttgaa tcacaaatcc gaatggagat tgagaacaag
    31921 gatgatttgt tacgaaattt gatgatccac tgtaaagcct tgttatttag aaagacttac
    31981 ggtatttttt ctaaaaatcc tctaacaaaa caaattcgat ccaagtatgg agaattattt
    32041 ttagtcacta gaaaatctgc ggaaatttta gaaggagcat ggtttattcg gctaacagac
    32101 gatgatattg cctatttgac gattcatatt ggaggatttt taaaatatac accatcgtct
    32161 caaaaaaata tgaaaaaagt ttatctcgtt tgtgatgaag gtgttgcggt ttcgagactt
    32221 ttgctgaaac aatgcaaact ttattttcca aatgagcaaa ttgacactgt atttacaaca
    32281 gaacaattta agagtgtgga agatattgca caagttgatg tagtgattac tactaatgat
    32341 gatttggata gcagatttcc gattttaagg gttaatccta tccttgaagc agaagatatt
    32401 ttgaaaatgc tagactatct taaacacaat atatttcgta ataagagcaa aagtttcagt
    32461 gaaaatcttt ctagtcttat ttcgtcttat attgtagaca gcaagttggc tagtaagttc
    32521 caagaagagg ttcaaacact tataaatcaa gaaatagtag ttcaagcttt tttggaagat
    32581 atttgaagga cagtccaatg atgaacacaa acctgtgttt ttcttggtct tttttagtgt
    32641 tttgaagggt ggtatactaa tctcaaagat aacaattata tccaaaggag gcaacatatg
    32701 ccaaacgtca aagaaattac aagagagtca tggattttag ccactttccc agagtgggga
    32761 acatggttga acgaagaaat cgaagaagaa gtcgtacctg aaggcaactt tgccatgtgg
    32821 tggctaggca actgtggtac ttggattaag acaccagctg gtgctaacgt tgtcatggac
    32881 ctttggtcaa accgtggaaa atcaaccaaa aaagtgaaag atatggttcg tgggcaccaa
    32941 atggcaaata tggcaggtgt tcgtaagttg caaccaaact tgcgtgttca gccaatggtt
    33001 atcgatccat ttgctatcaa cgaactagac tattacttag tttcacactt ccacagtgat
    33061 catatcgacc catacacagc tgcagcaatt ctcaacaatc ctaagttaga gcatgttaag
    33121 tttatcggtc cttaccactg tggacgaatc tgggaaggat ggggtgttcc aaaagaacgt
    33181 atcatcgttg ttaaaccagg tgacactatc gaattaaaag atatgaagat tcatgcagta
    33241 gaatcatttg accgtacttg cttggtaact ctcccagtga acggtgctga tgagacaggc
    33301 ggtgaacttg ctggcttggc tgttacagat gaagaaatgg ctcaaaaggc tgttaactat
    33361 atctttgaaa caccaggtgg aaccatctat catggtgcag attctcactt ctcaaactat
    33421 tttgcaaaac atggtaaaga ctttaaaatt gatgttgctt tgaataacta tggtgaaaat
    33481 ccggtaggta tccaagacaa aatgacatct atcgaccttc ttcgtatggc agaaaatctg
    33541 cgtaccaaag tcattatccc agttcactat gatatctggt ctaacttcat ggcttctact
    33601 aatgagattc tagaactttg gaaaatgcga aaagatcgct tgcaatacga tttccatcca
    33661 tttatctggg aagttggcgg taagtacact tatcctcaag atcaacactt agtagaatac
    33721 catcatccac gtggttttga tgattgtttt gaacaagact ctaacattca atttaaagct
    33781 ttgctataat ataaaaatat tctttggagg ttatccgtat gttgcatgat acggataatt
    33841 ttcatataat agtaaaatag aatgtgtgat tcaataatca cctcaaatag aaaggaaatt
    33901 ctatgtcaaa tctatctgtt aatgcaattc gttttctagg tattgacgcc attaataaag
    33961 ccaactcagg tcatccaggt gtggttatgg gagcggctcc gatggcttac agcctcttta
    34021 caaaacaact tcatatcaat ccagctcaac caaactggat taaccgcgac cgctttattc
    34081 tttcagcagg tcatggttca atgctccttt atgctcttct tcacctttct ggttttgaag
    34141 atgtcagcat ggatgagatt aagagtttcc gtcaatgggg ttcaaaaaca ccaggtcacc
    34201 cagaatttgg tcatacggca gggattgatg ctacgacagg tcctctaggg caagggattt
    34261 caactgctac tggttttgcc caagcagaac gtttcttggc agccaaatat aaccgtgaag
    34321 gctacaatat ctttgaccac tatacttacg ttatctgtgg agacggagac ttgatggaag
    34381 gtgtctcaag cgaggcagct tcatacgcag gtttgcaaaa acttgataag ttggttgttc
    34441 tttatgattc aaatgatatc aacttggatg gtgagacaaa ggattccttt acagaaagtg
    34501 ttcgtgaccg ttacaatgcc tacggttggc atactgcctt ggttgaaaat ggaacagact
    34561 tggaagccat ccatgctgct atcgaaacag caaaagcttc aggcaagcca tctttaattg
    34621 aagtgaagac ggttattgga tatggttctc caaacaaaca aggaactaat gctgtacacg
    34681 gcgcccctct tggagcagat gaaactgcat caactcgcca agccctcggt tgggactacg
    34741 aaccatttga aattccagaa caagtatatg ctgatttcaa agaacatgtt gcagaccgtg
    34801 gcgcatcagc ttatcaagct tggactaaat tagttgcaga ttataaagaa gctcatccag
    34861 aactggctgc agaagtagaa gccatcatcg acggacgtga tccagtcgaa gtgactccag
    34921 cagacttccc agctttagaa aatggttttt ctcaagcaac tcgtaattca agtcaagatg
    34981 ccttgaatgt tgtggcagct aaattaccaa ccttcctagg tggatcagct gaccttgctc
    35041 actcaaacat gacttatatc aagacggatg gacttcaaga cgataccaat cgcttgaatc
    35101 gtaacattca gtttggtgtt cgtgaatttg caatgggaac aatcttgaac gggatggccc
    35161 ttcatggtgg acttcgtgta tacggtggaa ctttcttcgt cttctctgat tatgtgaagg
    35221 cagctgtccg cttgtcagcc ttacaaggac ttcctgtgac ttatgtcttt acccatgatt
    35281 caatcgcagt tggggaagat ggtccgactc acgaaccagt tgagcattta gcaggtcttc
    35341 gtgctatgcc aaatctaaat gttttccgtc cagcagatgc gcgtgaaacg caagcagctt
    35401 ggtaccttgc agtgacaagt gagaaaacac caactgccct tgtcttgaca cgtcaaaact
    35461 tgactgttga agatggaaca gacttcgaca aggttgctaa aggtgcttat gttgtatatg
    35521 aaaatgcagc cgactttgat accatcttga ttgcgacagg ttcagaggtt aatcttgctg
    35581 tctcagctgc taaagaattg gctagtcaag gcgaaaaaat ccgcgtagtc agcatgccat
    35641 ctacagatgt ctttgataaa caagatgcag cttacaagga agagattctt ccaaatgcag
    35701 tccgccgtcg tgttgcagtc gaaatgggtg caagtcaaaa ctggtacaaa tatgttggtc
    35761 tcgatggtgc cgttctaggt attgatacct tcggagcctc tgccccagca ccaaaagtat
    35821 tggcagaata tggctttact gtagaaaatc ttgtaaaaat tgttcgaaac ttgaaataat
    35881 cctaaaaatc agggcgtaag ctctggtttt tcttaccaga aaagtaaggt acaatcttgt
    35941 aaaagtagct gaaatttgat atagtagtcc tatgtaaaag acaaaggaga aaacaatgaa
    36001 tccaaatatt acttttttaa tcatgcttgt aggtatgatg gccttgatgt tctttatgca
    36061 acgttctcaa aagaaacaag ctcaaaagcg tatggaaagc ttaaataaac tacaaaaagg
    36121 ctatgaagtg attacaatcg gtggacttta tggaacagtc gatgaagtag atacggagaa
    36181 gggaacaata gttcttgacg tagatggagt ttacttgact tttgaactag ctgctatcaa
    36241 gacagtatta ccgctgaaag aaacagcttc actagaaggc gcaattgaaa aataagacgg
    36301 gatcacgaac tcccgttttt ctataaaaga aaggaaatgg gatgaaaaaa ttagtctttg
    36361 tctgtctggg aaatatttgc cgtagcccta tggccgagtt tgttatgaaa tcaatgacag
    36421 ataactacga aatccaaagt cgagcaactt cctcttggga acatggcaat ccgattcata
    36481 aggggactca gggaattttt caacagtatg agattcctta tgacaagaac aagacatcgc
    36541 ttcagattag taaggaagat tttgaagcct ttgattatat tatcggaatg gacgcttcaa
    36601 atatttctga cttacgtcag atgtgtccag tagactgtca agataagatt tactcatttt
    36661 catctgaaag tgttccagac ccttggtata caggagattt tgaagaaacc tatcgacgtg
    36721 ttcaagaggg ctgtcaagct tggttagaac gtttagaaaa ggagagttaa ggtggaaaat
    36781 cttacgaatt tttacgaaaa gtatcgtgtc tatctgactc gtccacgttt agagcttttg
    36841 gcagtagtta ccattgtttt ctgtgctgta ctcgtctttt ttctaaatat tccaggaaaa
    36901 ggtgtcttaa aactcgataa tggaacgatt gtttatgatg gcagtcttgt ccgtggtaaa
    36961 atgaatggtc aaggtaccat taccttccaa aatggagacc aatatacagg tggcttcaac
    37021 aatggagcct tcaacggaaa aggtaccttt caatctaaag aaggctggac ctacgaaggt
    37081 gattttgtaa atggtcaggc tgaaggaaaa gggaaactaa caacagaaca agaagtcgtt
    37141 tatgaaggaa cttttaaaca aggcgttttt caacaaaaat aaagcctcct tatcaaagga
    37201 ggtattatta gaactacaag gtaagcgctt acctgtaaat ccctttcttt ccaaatccct
    37261 cttccaagca agtttgtgaa ataaaaaaca tttgaaataa atttcacaaa cttcaaagat
    37321 aaaacctgat aagaaaagaa aatgagaaaa gtttcacaag agtttaaaaa ttctttgtga
    37381 aatgtttatg aaactgttta caaagttgaa ataatacgtt ataataaact tgtgaagaaa
    37441 ttaacaaagg atatcgatcc ttgaaagcta tggaggaaaa tatggctgat aaaaaaactg
    37501 tgacaccaga ggaaaagaaa ctcgttgctg aaaaacacgt agatgagttg gttcaaaaag
    37561 ctctagttgc ccttgaagaa atgcgtaaat tggatcaaga acaagttgac tacatcgttg
    37621 ccaaagcatc agtagcagct ttggatgccc acggagaatt ggctttacat gcctttgaag
    37681 aaacaggacg tggtgtattt gaagacaaag caactaagaa cttgtttgcc tgtgaacacg
    37741 tagtaaacaa catgcgccac actaagacag ttggcgttat cgaagaagac gatgtaacag
    37801 gattgactct tattgctgaa ccagttggtg ttgtttgtgg tattactcca acaacaaacc
    37861 caacatcaac agcaatcttc aaatcattga tttcattgaa gacacgtaac ccaatcgtct
    37921 ttgccttcca tccatcagca caagaatcat ctgctcatgc agctcgtatc gtccgcgatg
    37981 cagctatcgc agctggtgct cctgaaaact gtgtgcaatg gattactcaa ccatctatgg
    38041 aagcaacaag tgcccttatg aaccacgaag gtgttgcgac aatccttgca acaggtggta
    38101 atgccatggt taaggcggct tattcatgtg gtaaaccagc tcttggggta ggtgccggaa
    38161 acgttccagc ttatgttgaa aaatcagcaa acattcgtca agcagcacac gatatcgtca
    38221 tgtctaaatc atttgataac ggtatggtct gtgcatctga acaagcagtt atcattgata
    38281 aagaaattta cgatgaattt gtagcagagt tcaaatctta ccacacttac tttgtaaaca
    38341 aaaaagaaaa agctcttctt gaagagttct gcttcggcgt caaagcaaac agcaaaaact
    38401 gtgctggtgc aaaattgaac gctgacatcg ttggtaaacc agcaacttgg attgcagaac
    38461 aagcaggatt tacagttcca gaaggaacaa acattcttgc tgcagaatgt aaagaagttg
    38521 gcgaaaatga gccattgact cgtgaaaaat tgtcaccagt tattgccgtt ttgaaatctg
    38581 aaagccgtga agatggtatt accaaggctc gtcaaatggt tgaatttaac ggtcttggac
    38641 actcagcagc tatccacaca gctgacgaag aattgactaa agaatttggt aaagctgtta
    38701 aagctattcg tgttatctgt aactcacctt ctacttttgg tggtatcggg gacgtttaca
    38761 atgccttctt gccatcattg acacttggat gtggttctta cggacgcaac tcagttgggg
    38821 ataacgttag tgccattaac ctcttgaata tcaaaaaagt cggaagacgg agaaataaca
    38881 tgcaatggat gaaacttcct tcaaaaacat actttgaacg tgattcaatt caataccttc
    38941 aaaaatgtcg tgacgttgaa cgtgtcatga tcgttactga ccatgccatg gtagagcttg
    39001 gtttccttga tcgtatcatc gaacaactgg accttcgtcg caataaggtt gtttaccaaa
    39061 tctttgcgga tgtagaaccg gatccagata tcacaactgt aaaccgtggt actgagatta
    39121 tgcgtgcctt caaaccagat accatcatcg cactcggtgg tgggtctcca atggatgctg
    39181 ccaaagtaat gtggctcttc tacgagcaac cagaagtgga cttccgtgac cttgtccaaa
    39241 aattcatgga tatccgtaaa cgtgccttca agttcccatt gcttggtaag aagactaaat
    39301 tcatcgcgat tccaactaca tctggtacag gatctgaagt aacaccattt gccgttatct
    39361 ctgataaagc aaacaaccgt aaatacccaa tcgctgacta ctcattgaca ccaactgtgg
    39421 caatcgtaga tcctgctttg gtattgacag ttccaggatt tgttgctgct gatactggta
    39481 tggacgtatt gactcacgcg acagaagcat acgtatcaca aatggctagt gactacactg
    39541 atggtttagc acttcaagct attaaattgg tctttgaaaa tctcgaaagc tcagttaaga
    39601 atgcagactt ccactcacgt gagaaaatgc ataacgcttc aacaatcgct ggtatggcct
    39661 ttgccaatgc cttcctaggt atttctcact caatggccca taagattggt gcgcaattcc
    39721 acacagttca cggacgtaca aatgccatct tgcttccgta cgttatccgt tacaacggta
    39781 cacgtccagc taagacagca acatggccta agtacaacta ctaccgtgca gatgaaaaat
    39841 accaagatat cgcacgcatg cttggacttc cagcttctac tccagaagaa ggggttgaat
    39901 cttacgcaaa agctgtctac gaactcggtg aacgtattgg gatccaaatg aattttagag
    39961 accaaggaat tgacgaaaaa gaatggaaag aacattctcg taaattagcc ttcctggctt
    40021 atgaagacca atgttcacca gctaacccac gtcttccaat ggtagaccat atgcaagaaa
    40081 tcatcgaaga tgcatactat ggctacaaag aaagaccagg acgccgtaaa taattgttta
    40141 tcagtctaga agcaagacaa aaactcaatt tgagggaaag atccagtaat ttttctatga
    40201 taaaaggcat cctatcaagg tttttgaaca cctgatagga tgccttttta tgatattgag
    40261 gcctttttgc cctttttgaa aaactagaat agaaacaaaa tatataatag attgaaacta
    40321 gagtagtaca catctgcttc taaaacattg ttagaa
//
LOCUS       DAVKJD010000012        35058 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKJD010000012 DAVKJD010000000
VERSION     DAVKJD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35058
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.012"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            66..320
                     /locus_tag="VW157_000652"
     CDS             66..320
                     /locus_tag="VW157_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9551989.1"
                     /translation="MHRTTIPCFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            664..1998
                     /gene="trmFO"
                     /locus_tag="VW157_000653"
     CDS             664..1998
                     /gene="trmFO"
                     /locus_tag="VW157_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9551990.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2082..2819
                     /locus_tag="VW157_000654"
     CDS             2082..2819
                     /locus_tag="VW157_000654"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9551991.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2828..3385
                     /gene="frr"
                     /locus_tag="VW157_000655"
     CDS             2828..3385
                     /gene="frr"
                     /locus_tag="VW157_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9551992.1"
                     /translation="MANIIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3445..4299
                     /gene="cvfB"
                     /locus_tag="VW157_000656"
     CDS             3445..4299
                     /gene="cvfB"
                     /locus_tag="VW157_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9551993.1"
                     /translation="MNTNLASFIVGLNIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4308..4523
                     /locus_tag="VW157_000657"
     CDS             4308..4523
                     /locus_tag="VW157_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9551994.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4609..5577
                     /locus_tag="VW157_000658"
     CDS             4609..5577
                     /locus_tag="VW157_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9551995.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(5555..5734)
                     /locus_tag="VW157_000659"
     CDS             complement(5555..5734)
                     /locus_tag="VW157_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551996.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            5770..6270
                     /locus_tag="VW157_000660"
     CDS             5770..6270
                     /locus_tag="VW157_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9551997.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6273..6533
                     /locus_tag="VW157_000661"
                     /pseudo
     CDS             6273..6533
                     /locus_tag="VW157_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6900..7226)
                     /locus_tag="VW157_000662"
     CDS             complement(6900..7226)
                     /locus_tag="VW157_000662"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9551998.1"
                     /translation="MVDVAVDQGGLIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTNLPVAQGLNRDYT
                     DINDLV"
     gene            complement(7172..7585)
                     /locus_tag="VW157_000663"
     CDS             complement(7172..7585)
                     /locus_tag="VW157_000663"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU9551999.1"
                     /translation="MGAHFLTKQAGGSGVLLGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLMSNSFNIEASVRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(7586..8011)
                     /locus_tag="VW157_000664"
     CDS             complement(7586..8011)
                     /locus_tag="VW157_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU9552000.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            8188..8757
                     /locus_tag="VW157_000665"
     CDS             8188..8757
                     /locus_tag="VW157_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9552001.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRC
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8824..9474
                     /locus_tag="VW157_000666"
     CDS             8824..9474
                     /locus_tag="VW157_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEU9552002.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLIVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9458..11698
                     /locus_tag="VW157_000667"
     CDS             9458..11698
                     /locus_tag="VW157_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9552003.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSTSYLALLGFVFLLVCL
                     FFQFPWKSASKVLVICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFFTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFIFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNTWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKNSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPYQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11877..12065
                     /locus_tag="VW157_000668"
     CDS             11877..12065
                     /locus_tag="VW157_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552004.1"
                     /translation="MTATKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            12097..12684
                     /locus_tag="VW157_000669"
     CDS             12097..12684
                     /locus_tag="VW157_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552005.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12688..13869
                     /locus_tag="VW157_000670"
     CDS             12688..13869
                     /locus_tag="VW157_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552006.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFK
                     V"
     misc_feature    14029..14160
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14176..14706
                     /gene="infC"
                     /locus_tag="VW157_000671"
     CDS             14176..14706
                     /gene="infC"
                     /locus_tag="VW157_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9552007.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14739..14939
                     /gene="rpmI"
                     /locus_tag="VW157_000672"
     CDS             14739..14939
                     /gene="rpmI"
                     /locus_tag="VW157_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9552008.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            14991..15350
                     /gene="rplT"
                     /locus_tag="VW157_000673"
     CDS             14991..15350
                     /gene="rplT"
                     /locus_tag="VW157_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9552009.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15408..15788
                     /locus_tag="VW157_000674"
     CDS             15408..15788
                     /locus_tag="VW157_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9552010.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYKDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16038..16838
                     /locus_tag="VW157_000675"
     CDS             16038..16838
                     /locus_tag="VW157_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9552011.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            16801..17787
                     /locus_tag="VW157_000676"
     CDS             16801..17787
                     /locus_tag="VW157_000676"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9552012.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKEFLR"
     gene            17829..19862
                     /locus_tag="VW157_000677"
     CDS             17829..19862
                     /locus_tag="VW157_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU9552013.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGAIGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGVKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(19923..21578)
                     /gene="pavA"
                     /locus_tag="VW157_000678"
     CDS             complement(19923..21578)
                     /gene="pavA"
                     /locus_tag="VW157_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9552014.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21683..22180
                     /gene="ybeY"
                     /locus_tag="VW157_000679"
     CDS             21683..22180
                     /gene="ybeY"
                     /locus_tag="VW157_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9552015.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22161..22556
                     /locus_tag="VW157_000680"
     CDS             22161..22556
                     /locus_tag="VW157_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9552016.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22573..23472
                     /gene="era"
                     /locus_tag="VW157_000681"
     CDS             22573..23472
                     /gene="era"
                     /locus_tag="VW157_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9552017.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23521..24345
                     /gene="mutM"
                     /locus_tag="VW157_000682"
     CDS             23521..24345
                     /gene="mutM"
                     /locus_tag="VW157_000682"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9552018.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24345..24950
                     /locus_tag="VW157_000683"
     CDS             24345..24950
                     /locus_tag="VW157_000683"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9552019.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRKQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24937..26136
                     /locus_tag="VW157_000684"
     CDS             24937..26136
                     /locus_tag="VW157_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU9552020.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26136..26285
                     /gene="rpmG"
                     /locus_tag="VW157_000685"
     CDS             26136..26285
                     /gene="rpmG"
                     /locus_tag="VW157_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9552021.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26325..26558
                     /gene="secG"
                     /locus_tag="VW157_000686"
     CDS             26325..26558
                     /gene="secG"
                     /locus_tag="VW157_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9552022.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26660..29014
                     /gene="rnr"
                     /locus_tag="VW157_000687"
     CDS             26660..29014
                     /gene="rnr"
                     /locus_tag="VW157_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9552023.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28977..29444
                     /gene="smpB"
                     /locus_tag="VW157_000688"
     CDS             28977..29444
                     /gene="smpB"
                     /locus_tag="VW157_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9552024.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29460..30320
                     /gene="tehB"
                     /locus_tag="VW157_000689"
     CDS             29460..30320
                     /gene="tehB"
                     /locus_tag="VW157_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9552025.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDIPVDLYDINSASIGQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30393..31346
                     /gene="coiA"
                     /locus_tag="VW157_000690"
     CDS             30393..31346
                     /gene="coiA"
                     /locus_tag="VW157_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9552026.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31365..33167
                     /gene="pepF"
                     /locus_tag="VW157_000691"
     CDS             31365..33167
                     /gene="pepF"
                     /locus_tag="VW157_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9552027.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGVYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYNFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            33169..33882
                     /locus_tag="VW157_000692"
     CDS             33169..33882
                     /locus_tag="VW157_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9552028.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            33949..34890
                     /gene="prsA"
                     /locus_tag="VW157_000693"
     CDS             33949..34890
                     /gene="prsA"
                     /locus_tag="VW157_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9552029.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
BASE COUNT        10548 a         6527 c         7932 g        10051 t
ORIGIN      
        1 gtcgtgcgct gaacaccaac aagacgctta aatcgtgcat cagttagttg tttacttgct
       61 tcatcattca tagaactact ataccatgtt ttgtttcgca ggaagtctat tggaaagtaa
      121 gaaatattga agctgaggcg attagaagaa attgtgagcg tggtgctatt ttttcaggta
      181 aaataaaaca tcacgaagat tctcagttta aaggagacca ctatgttgaa tgttatgctg
      241 ttttagataa tacggttata gcaagagata gaataacagt ccctatcgat ccgttatgtg
      301 gaaaagattt tatagagtag catataattg attcttaact ggaatactct ctatctcttt
      361 acatcaagaa aatgactaaa cagggaagtt tgccttcttc cctttttttg ttatactagt
      421 agaagaaaaa aatagaaaga tttgtgggag tgaaaaagtc cgggggactt tttcagcttg
      481 agccaagaaa ttcgagagcg aggaaagtcc gggggacttt ttcagcctga gccaagaaat
      541 tcgagagcga agtaagtcca atggactttt tcagcctggg ccaagtaact cgaaagctcg
      601 taagtccgat tggctttttc aatgtgaatc ttaacttcat actcccaaag aggtattagt
      661 gtcgtgtctc aatcttatat caatgttatc ggtgctggtt tggcaggttc tgaagcagcc
      721 taccaaatcg cagagcgtgg tattccagtt aaactttatg aaatgcgtgg tgtcaagtca
      781 acacctcagc acaaaacaga caactttgcc gagttggttt gttccaattc cttgcgtgga
      841 gatgctttga caaatgctgt tggcctcctt aaggaagaaa tgcgtcgctt gggttctgtg
      901 attttggaat ctgctgaggc tacacgtgtt cctgcaggtg gtgcccttgc agtggaccgt
      961 gatggtttct ctcaaatggt gaccgaaaaa gttgccaacc accccttgat tgaagtggtt
     1021 cgtgatgaaa ttacagaatt gccgacagat gttattacgg ttatcgctac tggtcctttg
     1081 acaagtgatg ccttggctga aaagattcat gcccttaatg acggtgatgg tttttatttc
     1141 tacgatgcgg cagcgcctat tatcgatgtc aacactatcg atatgagtaa ggtctatctc
     1201 aaatctcgtt atgataaggg agaagcggcc tacctcaatg cccctatgac caagcaagaa
     1261 tttatggatt tccatgaagc tttggtcaat gcagaagaag caccgcttaa ttcttttgaa
     1321 aaagaaaagt actttgaagg atgtatgcct atcgaagtca tggccaaacg tggcattaaa
     1381 accatgcttt atggtcctat gaaaccagtt ggtcttgagt atccggacga ctacacagga
     1441 cctcgcgatg gagaattcaa aaccccttat gcggttgtcc agctccgtca ggacaatgca
     1501 gccggtagcc tctacaatat tgttggtttc cagacccacc tcaaatgggg agaacaaaag
     1561 cgtgtcttcc aaatgattcc gggtcttgaa aatgcggagt ttgtccgtta tggtgtcatg
     1621 catcgcaatt cttacatgga ttcaccaaat cttcttgagc agacttaccg ttctaagaaa
     1681 caaccaaatc tcttctttgc tggtcagatg acgggggtgg aaggctatgt tgagtcggcg
     1741 gcttcgggct tagttgcggg aattaacgca gctcgtctct tcaaggaaga aagcgaggtt
     1801 attttccccg agacgacagc gattggaagc ttagctcatt acattaccca tgccgacagc
     1861 aaacatttcc aaccaatgaa tgtcaatttt gggatcatca aggagttgga aggcgagcgt
     1921 atccgtgata agaaggctcg ttatgaaaaa attgcagagc gtgcccttgc cgacttagag
     1981 gaatttttga ctgtctaatt tttttgaaag aattgctcat gatactataa aaatcttaga
     2041 aattgtgata aaataggtag gatgaaagaa ggagagtgaa aatggcgaat cccaagtata
     2101 aacgtatttt aatcaagtta tcaggtgaag cccttgccgg tgaacgtggc gtagggattg
     2161 atatccaaac agttcaaaca atcgcaaaag agattcaaga agttcatagc ttaggtatcg
     2221 aaattgccct tgttattggt ggaggaaatc tctggcgtgg agaacctgca gcagaagcag
     2281 gtatggaccg tgttcaggca gattacactg gaatgcttgg gactgttatg aatgctcttg
     2341 tgatggcaga ttcattgcaa caagttgggg ttgatacgcg tgtacaaaca gctattgcta
     2401 tgcaacaagt ggcagagcct tatgtccgtg gacgtgccct tcgtcacctt gaaaaaggcc
     2461 gtatcgttat ctttggtgct ggaattggtt caccatactt ctcgacagat acaacagcgg
     2521 cccttcgcgc agctgaaatc gaagcagatg ccatcctcat ggctaaaaat ggcgtcgatg
     2581 gtgtgtacaa tgccgatcct aagaaggaca agacagccgt taagtttgaa gaattgaccc
     2641 accgtgatgt tatcaacaaa ggtcttcgta tcatggactc aacagcctca accctctcaa
     2701 tggacaacga cattgacttg gttgtcttca acatgaacca atcaggcaac atcaaacgtg
     2761 tcgtatttgg tgaaaatatc ggaacaacag tttcaaataa tatcgaagaa aaggaataag
     2821 aaagaatatg gctaacataa ttattgaaaa agctaaagag agaatgaccc agtctcacca
     2881 atcacttgct cgtgaatttg gtggtatccg tgctggtcgt gccaatgcaa gcttgcttga
     2941 ccgtgtacat gtagaatact atggagtcga aactcctctt aaccaaatcg cttcaattac
     3001 gattccagaa gcgcgtgttt tgttggtaac accatttgac aagtcttcat tgaaagacat
     3061 cgaacgtgcc ttgaacgctt ctgatcttgg tatcacaccg gctaatgacg gttctgtgat
     3121 tcgcttggtt atcccagctc ttacagaaga aactcgtcgt gaccttgcta aagaagtgaa
     3181 gaaggtcggc gaaaatgcta aagtggctgt ccgcaatatc cgtcgcgatg ctatggacga
     3241 agctaagaaa caagaaaaag caaaagaaat cactgaagac gaattgaaga ctcttgaaaa
     3301 agatattcaa aaagtaacag acgatgctgt taaacacatc gacgacatga ctgctaacaa
     3361 agagaaagaa cttttggaag tctaaaaata aacagaaaaa ctcagttggc attgctggct
     3421 gagttttatt cgaaagaagg aaatatgaat acaaatcttg caagttttat cgttggactg
     3481 aacatcgatg aaaatgaccg tttttacttt gtgcaaaagg atggtcaaac ctatgctctt
     3541 gctaaggaag agggccaaca tacagtaggg gatacggtca aaggttttgc atacacggat
     3601 atgaagcaaa aactccgcct gacaacctta gaagtgactg ccactcagga ccaatttggt
     3661 tggggacgtg tcacagaggt tcgtaaggac ttgggtgtct ttgtggatac aggccttcct
     3721 gacaaggaaa tcgttgtgtc actcgatatt ctccctgagc tcaaggaact ctggcctaag
     3781 aagggcgacc aactctacat ccgtcttgaa gtggataaga aagaccgtat ctggggcctc
     3841 ttggcttatc aagaagactt ccaacgtctt gctcgtcctg cctacaacaa catgcagaac
     3901 caaaactggc cagccattgt ttaccgtctc aagctgtcag gaacttttgt ttacctacca
     3961 gaaaataata tgcttggttt tattcatcct agcgagcgtt acgcagagcc acgtttgggg
     4021 caagtattag atgcgcgcgt tattggtttc cgtgaagtgg accgcactct gaacctctcc
     4081 ctcaaaccac gctcctttga aatgttggaa aacgatgctc agatgatttt gacttatttg
     4141 gaaagtaatg gcggtttcat gaccttaaat gataagtcat ctccagacga catcaaggca
     4201 acctttggca tttctaaagg tcagttcaag aaggctttag gtggtcttat gaaggctggt
     4261 aaaatcaagc aggaccagtt tgggacagag ttgatttagg gaggcttatg agaaaatcat
     4321 tttacacttg gctcatgacc gagcgcaatc ctaaaagtaa cagtcccaaa gcaattttgg
     4381 cagacctcgc ttttgaagag gcagcttttc caaaacatac agatgatttt gatgaggtca
     4441 gtcgcttttt ggaggagcat gccagtttct cttttaacct aggagatttt gacagcattt
     4501 ggcaggaata tctagaacac tagcatttat tcattgggtt tgggctagta atttctccat
     4561 ccctctgcta taataaaaag aaataaaagg attagagagg ttctttattt gaaggaacat
     4621 tcaatagaca ttcaactgag tcatccagat gacctgtttc atctttttgg ttccaatgaa
     4681 cgccatcttc gtttgatgga agaagagctt gatgttgtga ttcatgctcg tacggagatt
     4741 gtccaggttt tgggagaaga gtctgcctgt gaggaagccc gtcaagttat tcaggctttg
     4801 atggtcttgg taaatcgtgg gatgaccgtt ggtacgccag atgtagtcac tgcgattagc
     4861 atggtcaaaa atgatgaaat tgacaagttt gtcgcccttt acgaagaaga aattatcaaa
     4921 gataatactg ggaaacctat ccgtgtcaaa accctagggc aaaagcttta tgtggacagt
     4981 gtcaaacagc atgatgtgac ctttggaatt gggccagcag gtacagggaa gaccttcctt
     5041 gcagtgacct tggcagtgac tgcccttaaa cgtgggcaag tcaagcgaat tatcctaact
     5101 cgtccagcgg tggaagcggg agagagtctt ggatttcttc cgggtgatct taaggagaag
     5161 gtggatcctt accttcgtcc tgtttacgat gccttgtatc aaattcttgg gaaagaccaa
     5221 acgactcgtc tcatggagcg tgaaattatc gaaattgcgc cccttgccta tatgcgtggc
     5281 cggaccttgg atgatgcctt tgtcattctc gatgaggcgc aaaacacgac catcatgcag
     5341 atgaagatgt tcttgacgcg tttaggcttt cattctaaga tgattgtcaa tggagatatc
     5401 agtcagattg acctgccacg taatgtcaag tccggtttga ttgatgctca agagaaactc
     5461 aagaacatcc atcagattga ctttgttcat ttttcagcca aggatgtggt tcgccatcca
     5521 gttgtcgctc agattatccg agcctatgaa tattctactg aagttgcaca cgactgattt
     5581 tgaggaagtt cgcctgcaaa agaatatact tttgagagag gaaaatccag ttgtataggc
     5641 taaaggtttt atccaaaggt ttgagacaac gattaggcac gatggaaaga acttttatgt
     5701 ggctgatgac gatcagtgca tcttcctgtg tcataatcac agggcacaag aaagtaggaa
     5761 tttgaaaaga tgattgacca actatctaag tattacagtt gtaggatact aactgaaaag
     5821 gatattccaa gtattttatc tttatatgaa agtaatcctc tgtattttca gcattgtcca
     5881 ccagagccaa attttgcaac tgtaaaagag gacatgcttt gtctacctga aggtaaagct
     5941 aaggctgata agttttttgt tggattttgg aatggatctg accttgtggc tgttatggat
     6001 tttgtctatg catatcctga tgaggagact gtttttattg gtttgtttat ggttgatcaa
     6061 gcctatcaga ggaaagggat tggtagtcat attgtgacag aagcactagc ttattttgct
     6121 aagaactttc gaaaggcacg tttggcttat gttaagggaa atccgcaatc tcagcatttt
     6181 tgggaaaagc agggctttaa atcaattgga tgcgaggtta agcaagaact ctatacggtt
     6241 gttatcgctg aacagagcct agaagattag aaatggcatc aagtaagaac tatttggaat
     6301 ttgttttgga acaattatca ggattagatg atgtgactta ccgttccatg atgggggagt
     6361 atattcttta cttccgcggc aagattattg gcggcattta tgacgatcgc tttttagtta
     6421 aacccgtgca agcagtctta gataagattg accaatcttc ttttgagttt ccatacaaag
     6481 gtgccaaaga aatgatttga gtggaagaac ttgataataa gatgtttcta taagacctga
     6541 ttttagctat gtataaccaa ctgccaacgc ccaaacctaa aaagaaaaag caagggtgaa
     6601 cgaagtaaaa aagaagtctg ctaaggccct gtctttgcac gggtaaaatt ttatatataa
     6661 aaagaagctg ggactaaaga gctcagcttc ctttggttta tataattgtc attacaagac
     6721 gaagtggttg ggcgaaactc tgttgacttt attcaattta gagtttctta tgcacaattg
     6781 agtctggaac gaaagtctcc agttgcaaag tatacagtac aataaaccaa cgatgtaata
     6841 gctgatgaca taaagcacag tgggtaggac ttgcgaagtc acccttttct tttcaaaatt
     6901 tatactaaat cattgatatc agtgtagtca cgattaagtc cttgagcaac tggtaggtta
     6961 gtcaagtaac cttgataagt agtcacacct tgacgcaagc cttcatcttc agagattgct
     7021 tgtgcgaatc ctttgccagc caaagcttcg atataaggaa gagtgacatt ggttagggcg
     7081 atggttgaag tgcgggcaac cgcaccaggg atattggcaa cggcatagtg gagaacaccg
     7141 tgtttttcat agacgggttc atcgtgcgtt gtcacacggt cagctgtttc gataaggcca
     7201 ccttggtcaa cagcaacgtc aacgatacag agcctggacg catttgtttg accatctcat
     7261 ctgtcaccaa ttccggtgct tttgcaccag ggatgagaat ggctccaatc accacatcag
     7321 catctctcac acttgcttca atgttgaatg aattagacat aagagtttga atttgacttc
     7381 caaagacttc ttctagaact gagagacgct tggcactaat atctaaaata gtcacttgag
     7441 caccaagacc aagggcgatg cgggcagcat gtgtaccgac gacaccatca ccgatgatag
     7501 ttacttttcc ttttggaaca cctggtacac caccaagtag aacaccagag ccaccagctt
     7561 gcttagtaag gaagtgagct ccgatttaaa cagccatacg acctgcaacc tcactcatag
     7621 gaacgaggag cggtagttgt ccttgattgt cacgaacagt ttcataggca attcctgttg
     7681 tttttgctgc taacatagca tctgctaatt ctggagcagc ggccatgtgc aagtaggtga
     7741 agagaagaag atcgtcgcgc aagtaaccgt attcagaact taaaggttct tttactttca
     7801 caaccaactc tgctgcccaa gcttcaccag cagtagcgac aatctcagct ccttgctttt
     7861 gatagtcagc atcagtaaag ccagaaccga gaccagcatt tgtttcgata aggacacgat
     7921 gaccacgact aactaagcta tgaacacctg caggtgtgag ggcgacacgg ttttcgttat
     7981 ttttaatttc ttttgggatt ccgattaaca ttgagataac ctacctttca attgacggtc
     8041 ttgttttggt tgtcacattc cagttcataa atcaaaaatg tgacggtttc attgtatatg
     8101 aaaccgcttc aaaaatcaag aaaaacttgt catccaaatt tttttatgct agactagtga
     8161 aaatcaagct ctaatggagg gaaaagtatg gaatcaatat ttgtgaaatt tgcccagtat
     8221 ccgtctatag aaacggagcg tttattgctt agacctgtaa ctttggatga tgcggaagca
     8281 atgtttgact atgcctcgga caagggtaat acacgttgca cttttccaac caatcaaagc
     8341 ttggaagaaa ccaagaataa cattgctcag ttctacttgg ctaatccctt gggacgttgg
     8401 gggattgaat taaaagataa cggcaagttt atcggaacta ttgacttaca caagattgat
     8461 cctgttctta agaaggcagc tattggctac attatcaata aaaagtattg gaatcaagga
     8521 ttaacgacag aagccaatcg tgctgtgatt gagctagctt ttgagaagat agggatgaat
     8581 aagttgactg cccttcacga taaggctaat cccgcgtcag gaaaggtcat ggagaaatca
     8641 ggcatgcgtt tttcccatgc agaaccatat gcttgtatgg accagcatga aaaaggccga
     8701 atcgtgacaa gagttcatta tgtcttgacc aaggaagact attttgcaaa taaataagca
     8761 gttgaaaaga aattttcgac tgttttttct tcctcttacg aataatttaa gagaggagaa
     8821 aatatggaag caattatcga gaaaatcaaa gagtataaaa tcatcgtcat ctgtactggt
     8881 ctgggcttga ttgtaggagg atttttcctg ctaaaaccag ctccacaaac acctgtcaaa
     8941 gagacgaatt tgcaggctga agtcgcagct gtttccaagg atttggtatc cgaaaaggaa
     9001 gtgaacaagg aagaaaagga agaacccctt gaacaagatc taatcacagt agatgtcaaa
     9061 ggtgctgtca aatcgccagg gatttatgac ttgcctgtag gtagtcgagt caatgatgct
     9121 gttcagaagg ctggtggctt gacagagcaa gcagacagca agtcgctcaa tctagctcag
     9181 aaagttagtg atgaggctct ggtttacgtt cctactaagg gagaagaagc agttagccaa
     9241 cagactggtt tggggacagc ttcttcaata agcaaggaaa agaaggtcaa tctcaacaag
     9301 gctagtctgg aagaactcaa gcaggtcaag ggactgggag gaaaacgagc tcaggacatt
     9361 atcgaccatc gtgaggcaaa tggcaagttc aaatcagtag acgagctcaa gaaggtctct
     9421 ggcattggtg ccaagactat agaaaagtta aaagactatg ttacagtgga ttaagaattt
     9481 ctctattccc ctaatttacc tgagttttct gttactttgg ctttactacg ccattttttc
     9541 aacatcctat cttgctttgt tgggatttgt ttttctgcta gtctgtctct ttttccaatt
     9601 tccttggaaa tctgctagca aagttctagt gatttgtgga atctttggat tttggtttgt
     9661 ttttcaaaat tggcaacaga gtcaagcgag tcaaaatctg gcggattctg ttgaaagggt
     9721 acggattttg cctgatacta ttaaggttaa tggtgatagt ctatcctttc gtggcaagtc
     9781 taacggtcgt gctttccaag tctattataa actccagtcc gaggaggaga aagaagcctt
     9841 tcaagcttta accgacctgc atgagatagg actagaaggg aagctttcgg agccagaagg
     9901 gcagagaaat tttggtggct ttaattacca agcctatctg aagactcagg gaatttacca
     9961 gactctcaat atcaaaacaa tccagtcact tcaaaagatt ggcagttggg atataggaga
    10021 aaacttgtcc agtttacgtc gaaaggctgt ggtttggatt aagacgcact ttccagaccc
    10081 tatgcgcaat tacatgacag gactcttgct gggacatctg gacaccgact ttgaggagat
    10141 gaatgagctt tattccagtc taggaattat ccacctcttt gccctatctg gcatgcaggt
    10201 aggttttttc atgaatggat ttaagaaact tctcttgcga ttgggcttga cccaagaaaa
    10261 gttgaaatgg ctgacttatc ccttttccct tatctatgcg ggactaactg gattttcagc
    10321 atcggttatt cgcagtctct tgcaaaagct actggctcaa catggggtta agggcttgga
    10381 taattttgcc ttgacggtgc ttgtcctctt tattgtcatg ccaaactttt tctttacagc
    10441 aggaggagtc ttgtcctgcg cttatgcttt tatcctgacc atgaccagca aagaagggga
    10501 ggggctcaag gctgttgcta gtgaaagtct agtcatctcc ttgggcatat tgcccattct
    10561 atccttctat tttgcggaat ttcaaccttg gtctatcctt ttgaccttta tcttttcctt
    10621 tctatttgac ttgaccttct taccgctctt gtctatttta tttgtccttt cctttctcta
    10681 tccagtcatt cagctgaact ttatctttga atggttagag ggcattattc gcttggtctc
    10741 gcaggtggca agtagaccgc ttgtctttgg acaacccaat acatggcttt taatcctatt
    10801 gttaatttct ttggctttgg tctatgattt gaggaaaaac attaaaaagc taacggtatt
    10861 gtgcttattg attacagggc tctttctcct gaccaagcat ccactggaaa atgaaatcac
    10921 catgctggat gtggggcaag gagaaagtat tttcctacgg gatgtaactg ggaaaaccat
    10981 tctcatagat gtaggtggta aggcagaatc ttataagaaa atcaaaaaat ggcaagaaaa
    11041 gatgacgacc agcaatgccc agcgaagctt gataccctat ctcaaaagtc gaggagtagc
    11101 taagattgac cagctaattt tgactaacac ggacaaggag catgttggag atttgtcaga
    11161 gatgaccaag gctttccatg taggggagat tctagtatca aaaaacagtc tgaaacagaa
    11221 ggaatttgtg gcagaactac aggcgactca aacaaaggtg cgtagtatga tagtagggga
    11281 gaacttgccc atttttggaa gtcagttaga agttctatct ccaaggaaaa tgggagatgg
    11341 aggacacgat gataccctag ttctgtatgg gaaattcttg gataagcaat ttctcttcac
    11401 gggaaatttg gaggagaaag gagagaagga cttgctgaag cactatccag acttgaaagt
    11461 aaatgttttg aaagctagcc aacatggcaa taaaaaatca tcaagtccag cctttctaga
    11521 aaaactcaaa ccagagctta ctcttatctc agttggaaag agcaatcgaa tgaaactccc
    11581 ctatcaggaa acattgacac gactggaagg tatcaatagc aaagtttatc gaactgacca
    11641 gcaaggagct atacgtttta aggggttgga tagttggaaa atcgaaagtg ttcgatagga
    11701 aggataaatg ttgtagatta gtgaaataaa ctaaaaattt gttgcataat aatgataaaa
    11761 atggtataat gaaaacgtat tcaatattga ggatataaaa tcattaaaaa tcagcaaaag
    11821 ttgttttatt agttagttta taatctattg gtcttcttca gtccagtgta tctgctgtga
    11881 cagccactaa aagttacaag tatgattgga atacggtttt ggaatatagt accaactatc
    11941 acgaccatca gtatgcttgg attccggaat ggtctcgtta ttacagctat tctgagtata
    12001 aagttggcgg aggctggaac tacgctcgtt atgaggtcat aaactactat actggaggtt
    12061 attaatcctt aaagagtgag aaaaggaggg ctggatatgt taaatcttac tcatgttacc
    12121 ttaaaaacgc gacaagtcat cttgcaagat gcggatttta cctttaaaaa gggtaggatt
    12181 tatggccttc ttgctatcaa tggctcggga aagacgacac tattccgagc tatgagcaag
    12241 ttgcttcccc ttagtagtgg acacatcgca gttcctcctt ctttgtttta ttatgagagc
    12301 gttgaatggc tggatggaaa cttaagtggg atggactacc ttcgtctcat aaaaaacatc
    12361 tggaagtcag acctaaactt gagagatgaa atcgcctact gggaaatggc tgactatatc
    12421 agtcttccca tccgcaagta ttccttaggg atgaagcaac gcttggtgat tgctatgtat
    12481 tttctcagtc aggccaaatg ctggctcatg gatgagatta caaatggctt agacgagtat
    12541 tatcgacaga agttttttga taggctagca caaatcgata gacaagaaca gctggttctt
    12601 ttaagttccc actataagga agagttggtt gatatctgcg atagagtagt aaccattcat
    12661 caggggcaga tagaagaggt ttagtttatg aaagatgtta gtctattttt attgaaaaaa
    12721 gttttcaaaa gtcgtttaaa ctggattatc ttacttttat ttgcatctgt actcggtgtt
    12781 accttttatt taaatagtca gactgcaaac tcacacagct tggagagcag gttggaaagt
    12841 cgcattgcag ccaacgagag ggctatcaat gaaaatgaag agaaactctc ccaaatgtct
    12901 gataccagct cggaggaata ccagtttgct aaaaataatt tagacgtgca aaaaaatctt
    12961 ttgacgcgaa agacagaaat tctaacttta ctaaaagaag ggcgctggaa agaagcctac
    13021 tatctgcagt ggcaagatga agagaagaat tatgaatttg tatcaaatga cccgactgct
    13081 agctctggct taaaaatggg ggttgaccgc gaacggaaga tttaccaagc cctgtatccc
    13141 ttgaacataa aagcacatac tttggagttt ccgacccacg ggattgatca gattgtctgg
    13201 attttagagg ttatcatccc aagcttgttt gtggttgcga ttatttttat gctaacacaa
    13261 ctatttgcag aaagatatca aaatcatctg gacacagctc acttatatcc tgtttcaaaa
    13321 gtgacatttg caatatcctc tcttggagtt ggagtgggat atgtaactgt gctgtttatc
    13381 ggaatctgtg gcttttcttt tctagtggga agtctgataa gtggttttgg acagttagat
    13441 tatccctacc caatttatag cttagtgaat caagaagtaa ctattgggaa aatacaagat
    13501 gtattatttc ctggcttgct cttagctttc ttagccttta tcgtcattgt ggaagttgtg
    13561 tacttgattg cttacttttt caagcaaaaa atgcctgtcc tctttctttc actcattggg
    13621 attgttggct tattgtttgg catccaaaca attcagcctc ttcaaaggat tgcacatctg
    13681 attcccttta cttacttgcg ttcagtggag attttatctg gaagattatc taagcagatt
    13741 gataatgtcg atctaaattg gagcatgggg atggtcttac ttccttgcct gattatcctt
    13801 ttgttagtgg ggattctatt tatcgaaagc tggggaagtt cacgtaaaaa ggaagttttt
    13861 aaagtttagt tttcctatag ggaagctaaa gaaaaactaa catagggaga gaatcaactt
    13921 gattctctct ttttgattcg aaaaccaaac caaaatacaa acacaaactt ttcaaaaaat
    13981 aactttttat cttgacaaga gctagaaaac ttggtatcat ttaaaagttg agaaaagcag
    14041 aagtgagagc ttctcgcctt gtgacattaa gttgcctggc cctacggatg aaaagtttcg
    14101 aagaaacgct atcataacgt gcgggcttgt atatttacga gtccgctatt gtttttctct
    14161 aataaaacaa aagaggtgaa aaccatagca aagcaagact tattcatcaa tgatgagatt
    14221 cgtgtacgtg aagttcgctt gattggtctt gagggagaac agctaggcat caagccactc
    14281 agtgaagcgc aagctttggc tgataacgct aatgttgacc tagtattgat tcaaccccaa
    14341 gccaaaccgc ctgttgcaaa aattatggac tacggtaagt tcaaatttga gtaccagaag
    14401 aagcaaaaag aacaacgtaa aaaacaaagc gttgttactg tgaaagaagt tcgtctaagt
    14461 ccaactattg acaagggtga ctttgataca aaacttcgta atgcacgtaa gttccttgaa
    14521 aaaggaaata aagttaaggt atctattcgc tttaagggtc gtatgattac ccataaagag
    14581 attggtgcaa aagttttagc cgagtttgct gaagcaactc aagatattgc catcatcgaa
    14641 caacgtgcta aaatggatgg acgtcaaatg ttcatgcaat tggcgccagc gactgacaaa
    14701 aaataattgt cagaaagtta aataaaggag aaaaaatcat gccaaaacaa aaaacacacc
    14761 gcgcatcagc taaacgtttc aaacgtacag gttctggtgg acttaaacgt ttccgtgctt
    14821 acacttctca ccgtttccac ggaaaaacta agaaacaacg tcgtcatctt cgtaaagcat
    14881 ctatggtgca ttcaggagat tacaaacgta tcaaagcaat gcttactcgc ttgaaataat
    14941 agcttgactg taagtaaaca attctaggaa atatttggag gaaataaaac atggcacgtg
    15001 ttaaaggtgg cgttgtatca cgcaaacgtc gtaaacgtat tcttaaatta gcaaaaggtt
    15061 actatggagc taaacacatc ttgttccgta ctgcaaaaga acaagtaatg aactcttact
    15121 actatgcata ccgtgaccgt cgtcagaaaa aacgtgactt ccgcaaattg tggattactc
    15181 gtatcaatgc ggcagctcgt atgaacggac tttcatactc acaattgatg catggtttga
    15241 aattggctga gatcgaagtt aaccgtaaaa tgcttgctga cttggctgtt aacgatgcgg
    15301 tagctttcac agctcttgca gatgcagcta aagcaaaact tggtaaataa caactgatga
    15361 gactggaaca ggattgtcca gtctttttaa aaataaagga gaaagatatg gcttcaaaaa
    15421 tgctacacac ttgcttacga gtagaaaatc ttgaaaaatc aatcgcattt tataaagatg
    15481 cttttggttt taaagaattg cgtcgcagag attttccaga ccatgccttc acgattgtct
    15541 atctaggtct tgagggtgat gattatgagt tggagttgac ttataactac gatcacggtc
    15601 cgtatgtggt tggtgatggg tttgcccata tcgccctcag tacacctgat cttgaggcac
    15661 ttcatcaaga acacagtgca aaaggctatg aagtgactga gccaaatggt ctaccaggaa
    15721 ctacaccaaa ctattacttt gtcaaagacc ctgatggata taaggtcgaa gtcattcgtg
    15781 aaaaataatt tcgtgcaagt taagtagaat attcgttttc tacttgactt tttttagctg
    15841 aaagagtata ctaataaaaa ttcaaccttt aaggggagtc cagagagact cacaaggtgt
    15901 cagataaaag aatagtgcaa ttttctagag gagacttttt gagtgtgctc tcttgtgttg
    15961 tacgatttta actgaggcct tgcactagca aggtcttttc tttatctggt ccccttaaaa
    16021 tttaaggagg aaaagttatg aatcccacat gtaagaagcg tttgggtgtc attcggttgg
    16081 aaaccatgaa ggtggttgca caagaggaaa tcgcgccagc aatctttgaa ttagtcctag
    16141 aaggagaaat ggttgaagcc atgcgagcag gccaatttct tcatctgcgt gtaccggacg
    16201 atgcccatct cttacgtcgt cctatttcaa tttcgtctat tgacaaggca aacaagcagt
    16261 gtcacctcat ttatcggatt gagggagctg ggactgcaat tttttcaacc ttaagtcagg
    16321 gagacactct tgatgtgatg gggcctcagg gaaatggttt tgacttgtct gaccttgatg
    16381 agcagaatca ggttctcctt gttggtggtg ggattggtgt tccacccttg cttgaggtgg
    16441 ccaaggaatt gcatgaacgt ggagtgaaag tagtgacagt cctcggtttt gctaataagg
    16501 atgctgttat tttgaaaacg gaattggctc agtatggtca ggtctttgta acgacagatg
    16561 atggttctta tggcatcaag ggaaatgttt ccgttgttat caatgattta gatagtcagt
    16621 ttgatgctgt ttactcgtgt ggggctccag gaatgatgaa gtatatcaat caaacctttg
    16681 atgatcaccc aagagcctat ttatctctgg aatctcgtat ggcttgtggg atgggagctt
    16741 gctatgcctg tgttctaaaa gtaccagaaa gcgagacggt cagccaacgc gtctgtgaag
    16801 atggtcctgt tttccgcaca ggaacagttg tattataagg agaaaattat gactacaaat
    16861 cgattacaag tgtctctacc tggtttggat ttgaaaaatc cgattattcc agcatcaggc
    16921 tgttttggct ttggacaaga gtatgccaag tactatgatt taaacctttt aggttctatt
    16981 atgatcaagg cgacaaccct tgaaccacgt tttgggaacc caactccaag agtagcagag
    17041 acgcctgctg gtatgctcaa tgcaattggc ttgcaaaatc ctggtttaga ggttgttttg
    17101 gctgaaaagc taccttggct ggaaagagaa tatccaaatc ttcctattat tgccaatgta
    17161 gctggttttt caaaacaaga gtatgcagct gtttctcatg ggatttccaa ggcaactaat
    17221 gtaaaagcta tcgagctcaa tatttcttgt cccaatgttg accactgtaa tcatggactt
    17281 ttgattggtc aagatccaga tttggcttat gatgtggtga aagcagctgt ggaagcctca
    17341 gaagtgccag tttatgtcaa attaaccccg agtgtgaccg atatcgttac tgtcgcaaaa
    17401 gctgcagaag atgcgggagc aagtggcttg accatgatca atactctggt tggaatgcgc
    17461 ttcgacctca aaaccagaaa accaatcttg gccaatggaa caggtggaat gtcaggtcca
    17521 gcagtctttc cagtagccct caaactcatc cgccaagtag cccaaacaac agacctgcct
    17581 atcattggaa tggggggagt ggattcggct gaagctgccc tagaaatgta tctggctggg
    17641 gcatctgcta tcggagttgg aacagctaac tttaccaatc cttatgcctg ccctgacatc
    17701 atcgaaaatt taccaaaagt catggataaa tacggtatta gcagtctgga agaactccgt
    17761 caggaagtaa aagagtttct gaggtaaact gcaatcaatc tgttcttgat tttttattag
    17821 tttgtaatat gaatttagga gaattttggt acaataaaat aaataagaac agaggaagaa
    17881 ggttaatgaa gaaagtaaga tttatttttt tagctctgct atttttctta gctagtccag
    17941 agggtgcaat ggctagtgat ggtacttggc aaggaaaaca gtatctgaaa gaagatggca
    18001 gtcaagcagc gaatgagtgg gtttttgata ctcattatca atcttggttc tatataaaag
    18061 cagatgctaa ctatgctgaa aatgaatggc taaagcaagg tgacgactat ttttacctca
    18121 aatctggtgg ctatatggcc aaatcagaat gggtagaaga caagggagcc ttttattatc
    18181 ttgaccaaga tggaaagatg aaaagaaatg cttgggtagg aacttcctat gttggtgcaa
    18241 taggtgccaa agtaatagaa gactgggtct atgattctca atacgatgct tggttttata
    18301 tcaaagcaga tggacagcac gcagagaaag aatggctcca aattaaaggg aaggactatt
    18361 atttcaaatc cggtggttat ctactgacaa gtcagtggat taatcaagct tatgtgaatg
    18421 ctagtggtgt caaagtacag caaggttggc tttttgacaa acaataccaa tcttggtttt
    18481 acatcaaaga aaatggaaac tatgctgata aagaatggat tttcgagaat ggtcactatt
    18541 attatctaaa atccggtggc tacatggcag ccaatgaatg gatttgggat aaggaatctt
    18601 ggttttacct caagtttgat ggcaaaatgg ctgaaaaaga atgggtctac gattctcata
    18661 gtcaagcctg gtactacttc aaatccggtg gttacatgac agccaatgaa tggatttggg
    18721 ataaggaatc ttggttttac ctcaaatctg atgggaaaat agctgaaaaa gaatgggtct
    18781 acgattctca tagtcaagct tggtactact tcaaatccgg tggttacatg acagccaatg
    18841 aatggatttg ggataaggaa tcttggtttt acctcaagtc tgatggcaaa atggctgaaa
    18901 aagaatgggt ctacgattct catagtcaag cctggtacta cttcaaatct ggtggctaca
    18961 tggcgaaaaa tgagacagta gatggttatc agcttggaag cgatggtaaa tggcttggag
    19021 gaaaagctac aaatgaaaat gctgcttact atcaagtagt gcctgttaca gccaatgttt
    19081 atgattcaga tggtgaaaag ctttcctata tatcgcaagg tagtgtcgta tggctagata
    19141 aggatagaaa aagtgatgac aagcgcttgg ctattactat ttctggtttg tcaggctata
    19201 tgaaaacaga agatttacaa gcgctagatg ctagtaagga ctttatccct tattatgaga
    19261 gtgatggcca ccgtttttat cactatgtgg ctcagaatgc tagtatccca gtagcttctc
    19321 atctttctga tatggaagta ggcaagaaat attattcggc agatggcctg cattttgatg
    19381 gttttaagct tgagaatccc ttccttttca aagatttaac agaggctaca aactacagtg
    19441 ctgaagaatt ggataaggta tttagtttgc taaacattaa caatagcctt ttggagaaca
    19501 agggcgctac ttttaaggaa gccgaagaac attaccatat caatgctctt tatctccttg
    19561 cccatagtgc cctagaaagt aactggggaa gaagtaaaat tgccaaagat aagaataatt
    19621 tctttggcat tacagcctat gatacgaccc cttacctttc tgctaagaca tttgatgatg
    19681 tggataaggg aattttaggt gcaaccaagt ggattaagga aaattatatc gataggggaa
    19741 gaactttcct tggaaacaag gcttctggta tgaatgtgga atatgcttca gacccttatt
    19801 ggggcgaaaa aattgctagt gtgatgatga aaatcaatga gaagctaggt ggcaaagatt
    19861 agtactataa gtgaatatga tttgagtgaa tagtaagtta aaaatcctga tttcaagtaa
    19921 aatcaggatt ttttcatgga tgcaattttt ttggagtctg gtgtgacgcg gagggtcttt
    19981 tgtcctgtgt aagtgacaaa gccgggtttt ccaccagttg gtttattgag ttttttgact
    20041 tcaatcatat ctacctgcac cagattcgac aggcgccctt gagagaagta ggcagctaac
    20101 tctgctgcgt ctgtcttgac tgcatcagat gggtcaagat ttcctgagat gacaacatgg
    20161 cttccaggaa tgtccttagc atggaaccaa agttcctcct tgcgggccat tttaaaggtc
    20221 aattcctcat tttgaagatt gtttcgtccg acatagatga tggttttgcc atcgcttgct
    20281 agatattgtt ctagtttttt gcgtttctgg attttctccc gttgtcttct gcggataaaa
    20341 cctgtttgaa tcaattcttc gcggatttca gcgatttctt ccagtccagc ttggttgagg
    20401 acggtttcta cactttccag atagagaata gtggctttgg tttcttcaat caaatcagtc
    20461 aagtatttga cagcttcttt gagtttctga taccgtttaa aatagcgttg ggcattctgg
    20521 ttgggagtca gagccttatc aagcgcaatc atgataggtt ggttggtata gtagttgtct
    20581 aggataacct ggtcttggtc gttaggcact tggtgtagga aggttgtcag caattctcct
    20641 ttttgacgaa attcttcagc gttgtctgtc gccagtaact ctttttcctg ttttttgagt
    20701 ttgtgtcggt ttttctgaag ttcattttca acacgacgaa tcagttcact ggcctgctgt
    20761 ttgacgcggt cgcgctcagc cttatcctta tagtaggtgt ccaacaaatc agaaagattt
    20821 gcaaaaggct ctcccacctg atttgcaaaa ggaactggac tgaaggaagt ctcagtcaag
    20881 catggcttgg tttcttgatt gaaaaaattt cggaaagcgg aaagtttttc actaaccagt
    20941 atcctttcca attcatttgc cgtatcgcgt cccagacctt gaaagaggct ttgaagattt
    21001 tttgctgtta gttcttgggt ttgcaggatt tcaaagagct tttcatcttt gatagtaaaa
    21061 ggattgagag attctgtact tggcggagcg atataggtcg atcctggaag taaggtgcgg
    21121 tagctatttt gtgaaaaacc gacgtgtttg ataacttcga ggattttatg actgctttta
    21181 tcgaccagta gaatattact gtgtttcccc ataatttcaa taatcaaggt agcctggata
    21241 tggtctccaa tctcgttttt attggaaact gtaatttcca caatacggtc attttccact
    21301 tgctcaatcg actcaatcag ggccccctgc aaatactttc tcaaaaccat gataaaggta
    21361 gaaggttgag ctggattttc aaaagtcgtt tgggtcagct gaatgcgtcc aaaaactgga
    21421 tgggcagaaa ggagcaggcg atggctttgg cgattgctgc ggatttgcaa gaccaactct
    21481 tgttcaaaag gctgattgat tttctggatg cgaccattca ctaattcgct tcgcaattcc
    21541 tcaactatgt ggtgtaaaaa aaatccgtca aatgacatcg ttctctcctt gtgattgtat
    21601 tccatagtat tatatcaaaa aggtagaata aaatcatgga aatgtggtat aataaagcca
    21661 agtaaagaga aacgagaagc acatgtatat tgaaatggta gatgaaactg gtcaagtttc
    21721 aaaagaaatg ttgcaacaaa cccaagaaat tttggaattt gcagcccaaa aattaggaaa
    21781 agaagacaag gagatggcag tcacttttgt gaccaatgag cgtagtcatg aacttaatct
    21841 ggagtaccgt gacaccgacc gtccgacaga tgtcatcagc cttgagtata aaccagaatt
    21901 ggaaattgcc tttgacgaag aggatttgct tgaaaatcca gaattggcag agatgatgtc
    21961 tgagtttgat gcctatattg gggaattgtt catctctatc gataaggctc atgagcaggc
    22021 cgaagaatat ggtcacagct ttgagcgtga gatgggcttc ttggcagtac acggcttttt
    22081 acatattaac ggctatgatc actatactcc ggaagaagaa gcggagatgt tcggtttaca
    22141 agaagaaatt ttgacagcct atggactcac aagacaataa acgaaaatgg aaaaatcgtg
    22201 acttgatatc cagtttagaa tttgccatca cagggatttt tactgctatc aaggaagaac
    22261 gaaatatgcg aaaacatgca gtgacggctc tagtggtcat ccttgcaggt tttgtttttc
    22321 aggtgtcacg aatcgaatgg ctctttcttc tattgagtat tttcttggta gtagcctttg
    22381 agattatcaa ctctgctatt gaaaatgtgg tggatttggc cagtcactat cacttttcca
    22441 tgctggctaa aaatgccaag gatatggcgg ccggcgcggt attagtggtt tctctttttg
    22501 cagccttaac aggcgcattg atttttctcc cacgaatctg ggatttatta ttttaaacag
    22561 taagaggaaa ttatgacttt taaatcaggc tttgtagcca ttttaggacg tcccaatgtt
    22621 gggaagtcaa cctttttaaa tcacgttatg gggcaaaaga ttgccatcat gagtgacaag
    22681 gcgcagacaa cgcgcaataa aatcatggga atttacacga ctgataagga gcaaattgtc
    22741 tttatcgaca caccagggat tcacaaacct aaaacagctc tcggagattt catggttgag
    22801 tctgcctaca gtacccttcg cgaagtggac accgttcttt tcatggtgcc tgctgatgaa
    22861 gcgcgtggta agggggacga tatgattatc gagcgtctca aggctgccaa ggttcctgtg
    22921 attttggtgg tgaataaaat cgataaggtc catccagacc agctcttgtc tcagattgat
    22981 gacttccgta atcaaatgga ctttaaggaa attgttccaa tctcagccct tcagggaaat
    23041 aacgtgtctc gtctagtgga tattttgagt gaaaatctgg atgaaggttt ccaatatttc
    23101 ccgtctgatc aaatcacaga tcatccagaa cgtttcttag tttcagaaat ggttcgcgag
    23161 aaagtcttgc acctaactcg tgaagagatt ccgcattctg tagcagtagt tgttgactct
    23221 atgaaacgag acgaagagac agacaaggtt cacatccgtg caaccatcat ggtcgagcgc
    23281 gatagccaaa aagggattat catcggtaaa ggtggcgcta tgcttaagaa aatcggtagt
    23341 atggcccgtc gtgatatcga actcatgcta ggagacaagg tcttcctaga aacctgggtc
    23401 aaggtcaaga aaaactggcg cgataaaaag ctagatttgg ctgactttgg ctataatgaa
    23461 agagaatact aagtagaggt aggctcatgc ctgcttcttg tttttacaga aggaggactt
    23521 atgcctgaat tacctgaggt tgaaaccgtt tgtcgtggct tagaaaaatt gattatagga
    23581 aagaagattt cgagtataga aattcgctac cccaagatga ttaagacgga tttggaagag
    23641 tttcaaaggg aattgcctag tcagattatc gagtcaatgg gacgtcgtgg aaaatatttg
    23701 cttttttatc tgacagacaa ggtcttgatt tcccatttgc ggatggaggg caagtatttt
    23761 tactatccag accaaggacc tgaacgcaag catgcccatg ttttctttca ttttgaagat
    23821 ggtggcacgc ttgtttatga ggatgttcgc aagtttggaa ccatggaact cttggtgcct
    23881 gaccttttag acgcctactt tatttctaaa aaattaggtc ctgaaccaag cgaacaagac
    23941 tttgatttac aggtctttca agctgccctt gccaagtcca aaaagcctat caaatcccat
    24001 ctcctagacc agaccttggt agctggactt ggcaatatct atgtggatga ggttctctgg
    24061 cgagctcagg ttcatccagc tagaccttcc cagactttga cagcagaaga agcgactgcc
    24121 attcatgacc agaccattgc tgttttgggc caggctgttg aaaaaggcgg ttccaccatt
    24181 cggacctata ccaatgcctt tggggaagat ggaagcatgc aggattttca tcaggtctat
    24241 gataagactg gtcaagaatg tgtacgctgt ggtaccatca ttgagaaaat tcaactaggc
    24301 ggacgaggca cccacttttg tccaaactgt caaaggaggg actgatgaga aaaatcatcg
    24361 gaatcactgg gggaattgcc tctggtaagt caactgtgac aaattttcta agaaagcaag
    24421 gctttcaagt agtggatgcc gacgcagtcg tccaccaact acagaaacct ggtggtcgtc
    24481 tgtttgaggc tctagtacag cactttgggc aagaaatcat tcttgaaaac ggagaactca
    24541 atcgccctct cctagctagt ctcatctttt caaatcctga agagcaaaaa tggtcgaagc
    24601 aaattcaagg ggagattatc cgtgaggaac tggctacttt gagagaacag ttggctcaga
    24661 cagaagagat tttcttcatg gatattcccc tactttttga gcaggactac agcgattggt
    24721 ttgctgagac ttggttggtc tatgtggacc gagatgccca agtagaacgc ttaatgaaaa
    24781 gggaccagtt gtccaaagat gaagctgagt ctcgtctggc agcccagtgg cctttagaaa
    24841 aaaagaaaga tttggccagc caggttcttg ataataatgg caatcagaac cagcttctta
    24901 atcaagtgca tatccttctt gagggaggta ggcaagatga cagagattaa ctggaaggat
    24961 aatctgcgca ttgcctggtt tggtaatttt ctgacaggag ccagtatttc tttggttgta
    25021 ccttttatgc ccatcttcgt ggaaaatcta ggtgtaggga gtgagcaagt cgctttttat
    25081 gcaggcttag caatttctgt ctctgctatt tccgcggcgc tcttttctcc tatttggggt
    25141 attcttgctg acaaatacgg ccgaaaaccc atgatgattc gggcaggtct tgctatgact
    25201 atcactatgg gaggcttggc ctttgtccca aatatctatt ggttaatctt tcttcgttta
    25261 ctaaacggtg tatttgcagg ttttgttcct aatgcaacgg cactgatagc cagtcaggtt
    25321 ccaaaggaga aatcaggctc tgccttaggt actttgtcta caggcgtagt tgcaggtact
    25381 ctaactggtc cctttattgg tggctttatc gcagaattat ttggcattcg tacagttttc
    25441 ttactggttg gtagttttct atttttagct gctattttga ctatttgctt tatcaaggaa
    25501 gattttcaac cagtagccaa ggaaaaggct attccaacaa aggaattatt tacctcggtt
    25561 aaatatccct atcttttgct caatctcttt ttaaccagtt ttgtcatcca attttcagct
    25621 caatcgatcg gccctatttt ggctctttat gtacgcgact tagggcagac agagaatctt
    25681 ctttttgtct ctggtttgat tgtgtccagt atgggctttt ccagtatgat gagtgcagga
    25741 gtcatgggca agctaggtga caaggtgggc aatcatcgtc tcttggttgt cgcccagttt
    25801 tattcagtca tcatctatct cctctgtgcc aatgcctcta gcccccttca actaggactc
    25861 tatcgtttcc tctttggctt gggaaccggt gccttgattc ccggggttaa tgccctactc
    25921 agcaaaatga ctcccaaagc cggcatttcg agggtctttg ccttcaatca ggtattcttt
    25981 tatctgggag gtgttgttgg tcccatggca ggttctgcag tagcaggtca atttggctac
    26041 catgctgtct tttatgcgac aagcctttgt gttgccttta gttgtctctt taacctgatt
    26101 caatttcgaa cattattaaa agtaaaggaa atctagtgcg agtaaaaatt aatctcaaat
    26161 gctcctcttg tgacagtatc aattacctaa ccagtaaaaa ttcaaaaacc catccagaca
    26221 agattgaggt tttaaagtat tgtcccaagg aaagaaaagt aaccctacat cttgaatcta
    26281 agtagatttt atggtaaaat aatagggatt ttaaggagtt tgatatgtat aacctattat
    26341 taaccatttt attagtatta tctgttgtga ttgtgattgc aattttcatg caaccaacca
    26401 aaaaccaatc cagcaatgta tttgatgcca gttcaggtga tttgtttgaa cgcagtaaag
    26461 ctcgcggttt tgaagctgta atgcagcgtt tgacagggat tttagtcttt ttctggctag
    26521 ccattgcctt agcattgacg gtattatcaa gtagataaga aaataatggg caggactagg
    26581 tctttgcctc tttttatttt taaaggatgt ttgagaaggt tttacagtaa aagaaaatta
    26641 aaaaatctag aaagaaaata tgaaagatag aataaaagaa tatttacaag acaagggaaa
    26701 ggtgactgtt aatgatttgg ctcaggcttt gggaaaagac agttccaagg attttcgtga
    26761 gttgattaaa accttgtcct taatggaaag aaagcaccaa attcgttttg aagaagatgg
    26821 tagtctgaca ttagaaatta agaaaaaaca tgagattacc ctcaagggga tttttcatgc
    26881 ccataaaaat ggttttggtt ttgtcagcct agaaggggaa gaggacgacc tttttgtagg
    26941 gaaaaatgat gtcaactatg ctattgatgg tgataccgtc gaggtagtga ttaagaaagt
    27001 cgctgaccgc aataagggaa cagcagcaga agccaaaatt attgatatcc tagaacacag
    27061 tttgacaaca gttgtcgggc aaatcgttct ggatcaggaa aagcctaagt atgctggcta
    27121 tattcgttca aaaaatcaga aaatcagtca accgatttat gttaagaaac cagccctaaa
    27181 gttagaagga acagaagttc tcaaggtctt tatcgataaa tacccaagca agaaacatga
    27241 tttctttgtt gcgagtgttc tcgatgtagt gggacactca acggatgtcg gaattgatgt
    27301 tcttgaggtc ttggaatcaa tggacattgt atccgagttt ccagaagctg ttgttaagga
    27361 agcagaaagt gtgcctgatg ctccgtctca aaaggatatg gaaggtcgtc tggatctaag
    27421 agatgaaatt acctttacca ttgacggtgc ggatgccaag gacttggacg atgcagtgca
    27481 tatcaaggct ctgaaaaatg gcaatctgga gcttggggtt cacatcgcag atgtttctta
    27541 ttatgtgacc gaggggtctg cccttgacaa ggaagccctt aaccgtgcga cttctgttta
    27601 cgtgacagac cgagtggtgc caatgcttcc agaacgacta tcaaatggca tctgctctct
    27661 caatccccaa gttgaccgcc tgacccagtc tgctattatg gagattgata aacatggtcg
    27721 tgtggtcaac tataccatta cacaaacagt tatcaagacc agttttcgta tgacctatag
    27781 cgatgtcaat gatatcctag ctggcgatga agaaaagaga aaagaatatc ataaaattgt
    27841 atcaagtatc gaactcatgg ccaagcttca tgaaacttta gaaaacatgc gtgtgaaacg
    27901 tggagctctc aattttgata ccaatgaagc gaagatttta gtggataaac aaggtaagcc
    27961 tgttgatatc gttcttcggc agcgtggtat tgccgagcgg atgattgagt cttttatgtt
    28021 gatggctaat gaaacagttg ccgaacattt cagcaagttg gatttgcctt ttatctatcg
    28081 aattcacgag gagcctaagg ctgaaaaggt tcagaagttt attgattatg cttcgagttt
    28141 tggcttgcgc atttatggaa ctgccagtga gattagtcag gaggcacttc aagacatcat
    28201 gcgtgctgtt gagggagaac cttatgcaga tgtattgtcc atgatgcttc ttcgctctat
    28261 gcagcaggct cgttattcgg agcacaatca cggccactat ggactagctg ctgactatta
    28321 tactcacttt accagtccaa ttcgtcgtta tccagacctt cttgttcacc gtatgattcg
    28381 ggattacggc cgttctaagg aaatagcaga gcattttgaa caagtgattc cagagattgc
    28441 gacccagtct tccaaccgtg aacgtcgtgc catagaagct gagcgtgaag tcgaagccat
    28501 gaaaaaggct gagtatatgg aagaatacgt gggtgaagag tatgatgcag ttgtatcaag
    28561 tattgtcaaa ttcggtctct ttgtcgaatt gccaaacaca gttgaaggct tgattcacat
    28621 cactaatcta cctgaatttt atcatttcaa tgagcgtgat ttgactcttc gtggagaaaa
    28681 atcaggtatc actttccgag tgggtcagca gatccgtatc cgtgttgaaa gagcggataa
    28741 aatgactgga gagattgatt tttcattcgt acctagtgag tttgatgtga ttgaaaaagg
    28801 cttgaaacag tctagtcgta gtggcagagg gcgtggttca aatcgtcgtt cggataagaa
    28861 ggaagacaag agaaaatcag gacgctcaaa tgataagcgt aagcattcac aaaaagacaa
    28921 gaagaaaaaa ggaaagaaac ctttttacaa ggaagtagct aagaaaggag ccaagcatgg
    28981 caaagggcga gggaaaggtc gtcgcacaaa ataaaaaggc acgccacgac tatacaatcg
    29041 tagatacgct agaggcaggg atggtcctga ctggaactga aatcaagagt gtacgagctg
    29101 ctcgaattaa tctcaaggat ggctttgctc aagtgaaaaa tggagaagtt tggctgagca
    29161 atgttcatat cgcgccttac gaagagggca atatctggaa ccaggaacca gaacgtcgtc
    29221 gtaaactcct gctccataaa aagcaaattc aaaaattgga acaagagacc aaagggacag
    29281 gaatgacctt agttcccctt aaggtctata taaaagatgg ctacgctaag cttcttttag
    29341 gacttgccaa agggaagcat gactatgaca aacgggagtc tatcaaacgt cgtgagcaaa
    29401 atcgagatat cgcgcgtgtg atgaaagctg ttaatcagcg ataaaaagag gaattgaaaa
    29461 tggaaaaatt agttgcctat aaacgcatgc ctttgtggaa taaacaaaca atgcctgaag
    29521 ctgttcagca aaagcacaat acaaaagttg ggacttgggg gaaaattact gtcttgaagg
    29581 gagctctcaa gtttattgaa ttgacagaag aaggggaagt tctagctgaa cacctctttg
    29641 aagcaggggc agacaatcca atggcccaac ctcaagcctg gcaccgagtg gaagctgcca
    29701 cagatgatgt ggaatggtac ttggaatttt attgtaagcc tgaggattat tttgctaaaa
    29761 aatacaatac caatcctgtt cattcagagg tcctagaggc catgcagaca gtgaaacaag
    29821 ggaaagcttt ggatttgggt tgtggtcagg ggcgtaattc tctttttcta gcccagcaag
    29881 attttgatgt gacggctgta gatcaaaatg gactggctct tgaaatcttg caaagcattg
    29941 tggagcagga agatttggac atacctgttg acctttacga tatcaattca gctagcattg
    30001 gacaagaata tgattttatc gtttcaacag ttgttctcat gtttctacaa gcggaccgca
    30061 ttccagctat tattcaaaat atgcaggaga aaaccagtgt tggtggttac aaccttatcg
    30121 tttgtgccat ggacacggag gattatcctt gctcggttaa tttcccattc acctttaaag
    30181 aaggagaact ggcagactat tacaaggatt gggaattggt taagtacaat gaaaatccag
    30241 gccatttgca tcgtcgcgat gagaatggca atcgtattca actacgcttt gcgaccttac
    30301 tagctaagaa aatcaagtaa acacacatga agattaggaa ttttcctgat cttttttctt
    30361 ttttacgaat gatatagaaa aggagggaat tcatgtttgt tgcgagagat gctaggggag
    30421 aattggtaaa tgtgttagag gataaacttg agaagcaagc atacacctgc ccagcttgtg
    30481 gaggccagct ccatttgcgt caaggaccaa gtgtacggac gcattttgcc cataaatcct
    30541 taaaagactg tgattttttc tttgaaaatg aaagtccaga acacctggcc aataaggaat
    30601 ccctctatca ctggttgaaa aaagagacaa aggttcaatt agagtacccg ctttcagaac
    30661 ttaaacagat tgcggatgta tttgtaaatg gcaatctagc tctagaagtt cagtgtagtc
    30721 ccttgcctca gaaagtcctt aaagagcgaa gtgagggata tcgtagtcag ggttaccaag
    30781 tactgtggtt gctgggtcaa aaactgtggc tcaaggagcg tttgactcgt ctacagcaag
    30841 gttttcttta tttcagtcaa aacatgggct tttatgtttg ggaattagac aaggaaaaac
    30901 aagttttaag actcaaatac ctgatttacc aggatctccg cggtaaactc cattatcaaa
    30961 tcaaggaatt ttcctatggt caaggtagtt tattggaaat attgcgtctt ccctataaga
    31021 gacaaaaaat atctcatttt acagtttctg aggacaagga catctgtcgc tatatccggc
    31081 aacaacttta ttatcaaaat ctcttttgga tgaaagaaca agcagaagcc tatcaaaagg
    31141 gagaaaatat cctgacttat ggactgaaag aatggtatcc acaaattcga ccaatagtgg
    31201 gcaaattttt ccagattgaa caagacttga ctagctatta tcagcacttt tatacctatt
    31261 accaaaaaaa tcctcaaaat gattggcaaa agctttatcc accagccttt tatcagcaat
    31321 atttcttgaa aaatatggta gaatagaaag gatggaggaa tctaatggta ttacaaagaa
    31381 atgaaataaa tgaaaaagat acatgggatc tatcaacgat ctacccaact gaccaggctt
    31441 gggaagaagc cttaaaagat ttaacagaac aattggagac agtagcccag tatgaaggcc
    31501 atctcttgga tagtgcggat aacctactag aaatcactga attttctctt gaaatggaac
    31561 gccagatgga gaagctttac gtttatgctc atatgaagaa tgaccaggat acacgtgaag
    31621 ctaagtatca agagtactat gccaaggcca tgacactcta cagccagtta gaccaagcct
    31681 tttcattcta tgatcctgaa tttatggaga ttagcgaaaa gcagtatgct gactttttag
    31741 aagctcaacc aaagctgcag gtttatcaac actattttga caagctcttg caaggcaagg
    31801 atcacgttct ttcacaacgt gaagaagaat tattggctgg agctggagaa atctttggtt
    31861 cagcaagtga aaccttcgct atcttggaca atgcggatat tgtgttccct tatgtcctag
    31921 acgatgatgg taaagaagtt cagctatctc atgggactta cacacgtttg atggagtcta
    31981 aaaaacgtga ggttcgccgt ggtgtctatc aagctcttta tgcgacttac gaacaattcc
    32041 aacacaccta tgccaaaacc ttgcaaacca atgttaaggt gcaaaattac cgtgctaaag
    32101 ttcgtaacta caagagtgct cgtcatgcag ccctcgcagc gaattttgtt ccagaaagtg
    32161 tttatgacaa tttggtagca gcagttcgca agcatttgcc actcttacat cgctatcttg
    32221 agcttcgttc aaaaatcttg gggatttcag atctcaagat gtacgatgtc tacacaccgc
    32281 tttcatctgt tgaatacaat tttacctacc aagaagcctt gaaaaaagca gaagatgctt
    32341 tggcagtctt gggtgaggat tacttgagcc gtgtcaaacg tgccttcagc gagcgttgga
    32401 ttgatgttta cgaaaatcaa ggcaagcgtt caggtgccta ctctggtggt tcttacgata
    32461 ccaatgcctt tatgcttctc aactggcagg acaatctgga caatctcttt actcttgttc
    32521 atgaaacagg tcacagtatg cattcaagct atactcgtga aactcagcct tatgtttacg
    32581 gagattactc tatctttttg gctgagattg cctcaactac caatgaaaat atcttgacgg
    32641 agaaattatt ggaagaagtg gaagacgacg caacacgctt tgctattctc aataacttcc
    32701 tagatggttt ccgtggaaca gttttccgcc aaactcaatt tgctgagttt gaacacgcca
    32761 ttcaccaagc agatcaaaat ggggaggtct tgacaagcga tttcctaaat aaactctacg
    32821 cagacttgaa ccaagagtat tatggtttga gtaaggaaga caatcctgaa atccaatacg
    32881 agtgggctcg cattccacac ttctactata actactatgt atatcaatat tcaactggct
    32941 ttgcggccgc ctcagccttg gctgaaaaaa ttgtccatgg tagtcaagaa gaccgtgacc
    33001 gctatatcga ctacctcaag gcaggtaagt cggactatcc acttaatgtc atgagaaaag
    33061 ctggtgttga tatggagaag gaagactacc tcaacgatgc ctttgcagtc tttgaacgcc
    33121 gtttaaatga gtttgaagcc cttgttgaaa aattaggatt ggcataaaat ggttgaatcg
    33181 tatagtaaga atgctaacca taacatgcgt cgtcctgtcg tcaaagaaga aattgtagac
    33241 ttgatgcgtc agcgtcaaaa gcaggtcaca ggtttcttga aagaattgga agactttgcc
    33301 cgcaaggaaa atattcctat tattccccat gaaacggttg cttatttccg ttttcttatg
    33361 gaaaccatgc agcctaaaaa tattctggaa attgggacgg ctatcggttt ttcagctctc
    33421 ttgatggctg aacatgcgcc aaatgctaag attacaacta ttgatcgtaa tccagaaatg
    33481 attggttttg ccaaggaaaa ttttgcccag tttgacagtc gcaagcaaat cactctccta
    33541 gagggagatg cggtggatgt cttatctaca ctgacagagt cttatgattt cgtctttatg
    33601 gattctgcca agtctaaata catcgtcttt ctgccagaaa tcctcaaaca tttggaagtt
    33661 ggtggtgtgg ttgtcttgga tgatattttt caaggtggtg atgttgccaa ggatattatg
    33721 gaagtccgtc gtggtcagcg aaccatttat cgaggccttc aaaaattatt tgatgcaacc
    33781 ttagacaatc cagaactcac cgcaacatta gtgcctttag gagatggtat tctcatgctt
    33841 cgtaaaaatg tagcagatgt tcaactgtct gaaagcgaat gattttcaga aaaatttaag
    33901 aaaaaatagt aaaatagata gagtaacact tatctcaaag gagtagacat gaagaaaaaa
    33961 ttattggcag gtgccatcac actattatca gtagcaactt tagcagcttg ttcgaaaggg
    34021 tcagaagggg cagaccttat cagcatgaaa ggggatgtca tcacagaaca tcaattttat
    34081 gagcaagtga aaaacaaccc ttcagcccaa caagtcttgt taaatatgac catccaaaaa
    34141 gtttttgaaa aacaatatgg ctcagagctt gatgataaag aggttgatga tactattgcc
    34201 gaagaaaaaa aacaatatgg cgaaaactac caacgtgtct tgtcacaagc aggtatgact
    34261 cttgaaacac gtaaagctca aattcgtaca agtaaattag ttgagttggc agttaagaag
    34321 gtagcagaag ctgaattgac agatgaagcc tataagaaag cctttgatga gtacactcca
    34381 gatgtaacgg ctcaaatcat ccgtcttaat aatgaagata aggccaaaga agttctcgaa
    34441 aaagccaagg cagaaggtgc tgattttgct caattagcca aagataattc aactgatgaa
    34501 aaaacaaaag aaaatggtgg agaaattacc tttgattctg cttcaacaga agtacctgag
    34561 caagtcaaaa aagccgcttt cgctttagat gtggatggtg tttctgatgt gattacagca
    34621 actggtacac aagcttacag tagccaatat tacattgtaa aactcactaa gaaaacagaa
    34681 aaatcatcta atattgatga ctacaaagaa aaattaaaaa ctgttatctt gactcaaaaa
    34741 caaaatgatt caacatttgt tcaaagcatt atcggaaaag aattgcaagc agccaatatc
    34801 aaggttaagg accaagcctt ccaaaatatc tttacccaat atatcggtgg tggagattca
    34861 agctcaagca gtagtacatc aaacgaatag tccaaatcaa tgagtcaggg aaaaaaactc
    34921 gacttcagga aaaaatgaag caaacattcc cacaataaaa cgcatagtac aaggtttgta
    34981 ctgcccccaa aagttagaca attaatttat ctgaaggatt tagttctgta ttgcacaggg
    35041 ctaagtcctt ttagtttt
//
LOCUS       DAVKJD010000013        33906 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKJD010000013 DAVKJD010000000
VERSION     DAVKJD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33906
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.013"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            640..1092
                     /locus_tag="VW157_000694"
     CDS             640..1092
                     /locus_tag="VW157_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9552030.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1111..2448
                     /locus_tag="VW157_000695"
     CDS             1111..2448
                     /locus_tag="VW157_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9552031.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQIMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2527..3411
                     /locus_tag="VW157_000696"
     CDS             2527..3411
                     /locus_tag="VW157_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9552032.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3411..4244
                     /locus_tag="VW157_000697"
     CDS             3411..4244
                     /locus_tag="VW157_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9552033.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4262..6355
                     /gene="nanB"
                     /locus_tag="VW157_000698"
     CDS             4262..6355
                     /gene="nanB"
                     /locus_tag="VW157_000698"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU9552034.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPALIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGDSQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6367..7470
                     /locus_tag="VW157_000699"
     CDS             6367..7470
                     /locus_tag="VW157_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9552035.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMQGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7643..8341
                     /locus_tag="VW157_000700"
     CDS             7643..8341
                     /locus_tag="VW157_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9552036.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8360..9877
                     /locus_tag="VW157_000701"
     CDS             8360..9877
                     /locus_tag="VW157_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9552037.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFLGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            10002..11330
                     /locus_tag="VW157_000702"
     CDS             10002..11330
                     /locus_tag="VW157_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9552038.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11582..12448
                     /locus_tag="VW157_000703"
     CDS             11582..12448
                     /locus_tag="VW157_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9552039.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12464..13303
                     /locus_tag="VW157_000704"
     CDS             12464..13303
                     /locus_tag="VW157_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9552040.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13576..14028
                     /locus_tag="VW157_000705"
     CDS             13576..14028
                     /locus_tag="VW157_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9552041.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYASMEEKVRKYLFKILID"
     gene            14169..14477
                     /locus_tag="VW157_000706"
     CDS             14169..14477
                     /locus_tag="VW157_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552042.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGTYAAKYGISMARSI
                     LNRAAATAAARVRLLTKIFGWILRIAVLAADVYGNFANNIAAAWDAHDKIPNNGRINF
                     "
     gene            14764..15408
                     /locus_tag="VW157_000707"
     CDS             14764..15408
                     /locus_tag="VW157_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9552043.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEIKKSRRVPVFKIYLRSFKQNLKLGLQLGLLELGIVSLSLLDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEVLQKGLILASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15577..16494
                     /locus_tag="VW157_000708"
     CDS             15577..16494
                     /locus_tag="VW157_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9552044.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16512..17396
                     /locus_tag="VW157_000709"
     CDS             16512..17396
                     /locus_tag="VW157_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9552045.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17412..18263)
                     /locus_tag="VW157_000710"
     CDS             complement(17412..18263)
                     /locus_tag="VW157_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9552046.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18463..20520
                     /gene="pbp2b"
                     /locus_tag="VW157_000711"
     CDS             18463..20520
                     /gene="pbp2b"
                     /locus_tag="VW157_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9552047.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20531..21127
                     /gene="recR"
                     /locus_tag="VW157_000712"
     CDS             20531..21127
                     /gene="recR"
                     /locus_tag="VW157_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9552048.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21303..22346
                     /locus_tag="VW157_000713"
     CDS             21303..22346
                     /locus_tag="VW157_000713"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9552049.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPVGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNVGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22430..23803
                     /locus_tag="VW157_000714"
     CDS             22430..23803
                     /locus_tag="VW157_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9552050.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTTYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLILAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23898..24602
                     /locus_tag="VW157_000715"
     CDS             23898..24602
                     /locus_tag="VW157_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9552051.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            24821..26194
                     /gene="ftsA"
                     /locus_tag="VW157_000716"
     CDS             24821..26194
                     /gene="ftsA"
                     /locus_tag="VW157_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9552052.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDTAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26211..27470
                     /gene="ftsZ"
                     /locus_tag="VW157_000717"
     CDS             26211..27470
                     /gene="ftsZ"
                     /locus_tag="VW157_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9552053.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKRTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27475..28146
                     /locus_tag="VW157_000718"
     CDS             27475..28146
                     /locus_tag="VW157_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9552054.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28156..28695
                     /locus_tag="VW157_000719"
     CDS             28156..28695
                     /locus_tag="VW157_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9552055.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            28695..28958
                     /locus_tag="VW157_000720"
     CDS             28695..28958
                     /locus_tag="VW157_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9552056.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            28955..29740
                     /locus_tag="VW157_000721"
     CDS             28955..29740
                     /locus_tag="VW157_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEU9552057.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELNLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLFQDKGQTKKEKKKITVQLLLSK"
     gene            29749..30537
                     /gene="divIVA"
                     /locus_tag="VW157_000722"
     CDS             29749..30537
                     /gene="divIVA"
                     /locus_tag="VW157_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9552058.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            30885..33677
                     /locus_tag="VW157_000723"
     CDS             30885..33677
                     /locus_tag="VW157_000723"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9552059.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLTQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTIERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10461 a         5989 c         7352 g        10104 t
ORIGIN      
        1 aaaagatttg aaactaaatt ccaaattaga aaaagacttg aaatactaaa aaaaaaaagt
       61 atactttaat tgaaaacggt aacaaaacta atttagagaa tgaaatatag agtatttctc
      121 tcttaaaagt ttttggtgaa acgagatgta gaaaggagat ttagccaaag agtctattag
      181 tgctagaata atagattaga attattttag aaaaacgaag tgagcagctt ataaattcaa
      241 gtccccaaat agattcatac tagtatcttt tgcaaaaaat aaagggcgac ttccttcatg
      301 aatatcaatt tcatttataa ggaaggtagc taattgaact aacttattta ttctgtttgt
      361 cgctagaaaa atcagacctc cttgtgaaga ttgaggagat actgaatgaa aatcaaagaa
      421 gaaactagca agctagtagc agattgccca aaacaccgct ttgaggttgt agataagact
      481 gacgtatata atccaaggtg aagcgactgt ggtttgaaga gattttcaaa gagtataggc
      541 tagagagtag tgtttttatg tccttctagt agaaaatgct agacagaaga atggggaact
      601 tggataggaa aaatagattg agaaaggagg ttagaagaga tgattattac aaaaattagc
      661 cgtttaggaa cttatgtggg agtcaatcca cattttgcaa cattaataga ttttctagaa
      721 aaaacaggac tagaaaattt aacagaaggt tcgattgcta tcgatggtaa tcgattgttt
      781 gggaattgct ttacttatct agcagatggt caagcagggg ctttctttga aacacaccaa
      841 aaatatttgg atattcattt agttttggaa aacgaagaag ccatggctgt tacatcgccg
      901 gaaaatgtaa gcgttaccca agaatatgat gaagagaaag atattgaatt atacacaggg
      961 aaagtggaac agttggttca tttgagagct ggtgaatgcc tcatcacttt tccagaagat
     1021 ttacatcaac ccaaggttcg tataaatgat gaacctgtga aaaaagttgt ctttaaagtt
     1081 gcgatttctt aatgtagaaa gagaagaacg atgaaaaaaa tgagaaagtt tttatgtcta
     1141 gctggaattg cgctagcggc tgttgccttg gtagcttgtt caggaaaaaa agaagctaca
     1201 actagtactg aaccaccaac agaattatct ggtgagatta caatgtggca ctcctttact
     1261 caaggacccc gtttagaaag tattcaaaaa tcagcagatg ctttcatgca aaagcatcca
     1321 aaaacgaaaa tcaagattga aacattttct tggaatgact tctatactaa atggactaca
     1381 ggtttagcaa atggaaatgt gccagatatc agtacagctc ttcctaacca agtaatggaa
     1441 atggtcaact cagatgcttt ggttccgcta aatgattcta tcaagcgtat tggacaagat
     1501 aaatttaacg aaactgcctt aaatgaagca aaaatcggag atgattacta ctctgttcct
     1561 ctttattcac atgcacaaat catgtgggtt agaacagatt tgttaaaaga acataatatt
     1621 gaggttccta aaacttggga tcaactctat gaagcttcta aaaaattgaa agaagctgga
     1681 gtttatggct tgtctgttcc gtttggaaca aatgacttaa tggcaacacg tttcttgaac
     1741 ttctacgtac gtagtggtgg aggaagcctc ttaacaaaag atcttaaagc agacttgaca
     1801 agccaacttg ctcaagatgg tattaaatac tgggttaaat tgtataaaga aatctcacct
     1861 caagattctt tgaattttaa tgtccttcaa caagctacct tgttctatca aggaaaaaca
     1921 gcatttgact ttaactctgg cttccatatc ggaggaatta atgccaacag tcctcaattg
     1981 attgattcga ttgatgctta tcctattcca aaaatcaaag agtctgataa agaccaagga
     2041 attgaaacct caaacattcc aatggttgtt tggaaaaatt caaaacatcc agaagttgct
     2101 aaagcattct tagaagcact ttataatgaa gaagactacg ttaaattcct tgattcaact
     2161 ccagtaggta tgttgccaac tattaagggg attagcgatt ctgcagccta taaagaaaat
     2221 gaaactcgta agaagtttaa acatgctgaa gaagtaatta ctgaagctgt taaaaaaggt
     2281 actgctattg gttatgaaaa tggaccaagt gtacaagctg gtatgttgac taaccaacac
     2341 attattgaac aaatgttcca agatatcatt acaaatggaa cagatcctat gaaagcagca
     2401 aaagaagcag aaaaacaatt aaacgattta tttgaggctg ttcagtagat gtaaaagact
     2461 agaaaatagg tgggctagtg atctgaaaag ctctagccca attttgtaaa agaagggaga
     2521 aggagaatgg ttaaagaacg taatttaact cgctggatat ttgttttgcc agctatgatt
     2581 atcgtaggat tactctttgt ttatccgttt ttctcgagta ttttttatag ctttaccaat
     2641 aagcatttga ttatgcctaa ttataaattt gttggtttgg ctaactataa agctgtgcta
     2701 tcagatccca acttctttaa tgcgttcttt aattcaatta agtggaccgt tttctcatta
     2761 gttggtcaag ttttagtagg gtttgtattg gctttagctc ttcacagagt acgccacttc
     2821 aagaaattat ataggacctt attgattgtt ccttgggcat ttcctaccat cgttattgcc
     2881 ttctcttggc agtggattct aaacggggtt tatggctact tacctaatct aatcgtaaaa
     2941 ttaggtttaa tggaacatac acctgcattt ttgacagata gtacatgggc attcctatgt
     3001 ttggtgttta tcaacatttg gtttggagca ccaatgatta tggttaatgt gctttcagct
     3061 ttgcaaacag taccagaaga acaatttgag gctgctaaga tagatggtgc ttcaagttgg
     3121 caggtgttca agtttatcgt ctttccacat attaaagtgg ttgtaggact tctagttgtt
     3181 ttgagaactg tatggatctt taataacttt gacattatct acctcattac tggtggtgga
     3241 ccagccaatg ctacaacgac gcttccaatt tttgcttaca acctgggctg gggaactaaa
     3301 ttgttgggtc gtgcttcagc agttacagta ctgctcttta tcttcttggt ggcgatttgc
     3361 tttatctact ttgctatcat cagtaagtgg gaaaaggagg gtagaaaata atgaagaaga
     3421 aatccagtat ttatttagat attctctcac atgtactttt agttggtgcg accatcgttg
     3481 cagttttccc attggtatgg attatcatat cttctgtcaa agggaaaggg gaattaactc
     3541 agtatccaac acgattttgg cctgaacagt ttacattaga ttatttcact catgttatca
     3601 acgatttgca cttcattgat aacattcgaa acagtttaat cattgccttg gctacaaccc
     3661 ttattgcgat tattatttct gctatggcag cctatggtat tgttcgattc tttcctaaat
     3721 tgggagcaat catgtcgaga ctactcgtca ttacctacat tttcccacca attttgttag
     3781 caattcccta ttcaattgcc attgctaaag ttgggttaac aaatagttta tttggcttga
     3841 tgatggttta tctatctttt agtgttccat atgcagtttg gctcttagtt ggatttttcc
     3901 aaacagttcc aattggaatt gaagaagcgg ctagaattga tggtgcaaat aaatttgtta
     3961 cgttttataa agttgtgcta ccgattgtag caccaggtat tgtagcaaca gctatttata
     4021 catttatcaa tgcttggaat gaattcctgt atgccttgat tttgattaac aatacaggaa
     4081 agatgacagt agcagtagcc cttcgttcac ttaatggttc agaaatacta gactggggag
     4141 atatgatggc agcgtctgtt attgtagttc ttccatcaat tattttcttc tctatcatcc
     4201 aaaataagat tgcaagtgga ttatcagaag gatctgtgaa gtagacgaaa gaaggaaaaa
     4261 aatgaataaa agaggtcttt attcaaaact aggaatttct gttgtaggca ttagtctttt
     4321 aatgggagtc cccgctttga ttcatgcgaa tgaattaaac tatggtcaac tgtccatatc
     4381 tcctattttt caaggaggtt catatcaact gaacaataag agtatagata tcagcccttt
     4441 gttattagat aaattgtctg gagatagtca gacagtagta atgaaattta aagcagataa
     4501 accaaactct cttcaagctt tgtttggcct atctaatagt aaagcaggct ttaaaaataa
     4561 ttacttttca attttcatga gagattctgg tgagataggt gtagaaataa gagacgccca
     4621 aaagggaata aattatttat tttctagacc agcttcatta tggggaaagc ataaaggaca
     4681 ggcagttgaa aatacactag tatttgtatc tgattctaaa gataaaacat acacaatgta
     4741 tgttaatgga atagaagtgt tctctgaaac agttgataca tttttgccaa tttcaaatat
     4801 aaatggtata gataaggcaa cactaggagc tgttaatcgt gaaggtaagg aacattacct
     4861 cgcaaaagga agtattgatg aaatcagtct atttaacaaa gcaattagtg atcaggaagt
     4921 ttcaaatatt cccttgtcaa atccatttca gttaattttc caatcaggag attctactca
     4981 agctaactat tttagaatac cgacactata tacattaagt agtggaagag ttctatcaag
     5041 tattgatgca cgttatggtg ggactcatga ttctaaaagt aagattaata ttgccacttc
     5101 ttatagtgat gataatggga aaacgtggag tgagccaatt tttgctatga agtttaatga
     5161 ctatgaggag cagttagttt actggccacg agataataaa ttaaagaata gtcaaattag
     5221 tggaagtgct tcattcatag attcatccat tgttgaagat aaaaaatctg ggaaaacgat
     5281 attactagct gatgttatgc ctgcgggtat tggaaataat aatgcaaata aagccgactc
     5341 aggttttaaa gaaataaatg gtcattatta tttaaaacta aagaagaatg gagataacga
     5401 tttccgttat acagttagag aaaatggtgt cgtttatgat gaaacaacta ataaacctac
     5461 aaattatact ataaatgata agtatgaagt tttggaggga ggaaagtctt taacagtcga
     5521 acaatattcg gttgattttg atagtggctc tttaagagaa aaacataatg gaaaacaggt
     5581 ccctatgaat gttttctaca aagattcgtt atttaaagtg actcctacta attatatagc
     5641 aatgacaact agtcagaata gaggagagag ttgggaacaa tttaagttgt tgcctccgtt
     5701 cttaggagaa aaacataatg gaacttactt atgtcccgga caaggtttag cattaaaatc
     5761 aagtaacaga ttgatttttg caacatatac tagtggagaa ctaacctatc tcatttctga
     5821 tgatagtggt caaacatgga agaaatcctc agcttcaatt ccgtttaaaa atgcaacagc
     5881 agaagcacaa atggttgaac tgagagatgg tgtgattaga acattcttta gaaccactac
     5941 aggtaagata gcttatatga ctagtagaga ttctggagaa acatggtcgg aagtttcgta
     6001 tattgatgga atccaacaaa cttcatatgg cacacaagta tctgcaatta aatactctca
     6061 attaattgat ggaaaagaag cagtcatttt gagtacacca aattctagaa gtggccgtaa
     6121 gggaggtcaa ttagttgtcg gtttagtcaa taaagaagat gatagtattg attggaaata
     6181 ccactatgat attgatttgc cttcgtatgg ttatgcctat tctgcgatta cagaattgcc
     6241 aaatcatcac ataggtgtac tgtttgaaaa atatgattcg tggtcgagaa atgaattgca
     6301 tttaagcaat gtagttcagt atatagattt ggaaattaat gatttaacaa aataaaggag
     6361 aaaaacatgg ttaaatacgg tgttgttgga gcagggtatt ttggagctga attggctcgc
     6421 tacatgcaaa aaaatgatgg agcagagatt actcttctct atgatccaga taatgcagag
     6481 gcgattgcag aagaattggg agcaaaagta gcaagttcct tagatgagtt ggtttctagc
     6541 gatgaagtag actgtgttat cgtcgcaact ccaaataatc ttcataagga accggttatt
     6601 aaggctgcac agcatggtaa aaatgttttc tgtgaaaaac caattgcgct ttcttatcaa
     6661 gattgtcgcg agatggtaga tgcgtgtaaa gaaaacaatg taacctttat ggcaggacat
     6721 attatgaatt tctttaatgg tgttcatcat gcaaaagaac tcattaatca aggagttatc
     6781 ggagacgttc tatattgtca tacagctcgt aatggttggg aagaacaaca accgtcagta
     6841 tcatggaaaa aaattcgtga aaaatcaggt ggtcacttgt atcaccacat ccatgaattg
     6901 gattgcgttc aattccttat ggggggcatg cctgaaactg taaccatgac aggtggaaat
     6961 gtggcccatg aaggtgaaca tttcggtgat gaagatgata tgatttttgt caatatggaa
     7021 ttttctaata agcgttttgc cttgttagaa tggggttcag cttatcgttg gggtgaacat
     7081 tatgtcttaa tccaaggaag caaaggtgcc atccgcttag acttattcaa ctgtaaagga
     7141 actcttaagc tagatgggca agaaagctat ttcttgattc acgaatcgca agaagaagat
     7201 gatgatcgga ctcgtatcta tcatagtaca gagatggatg gagcaattgc ttatggtaaa
     7261 ccaggtaaac gtactccatt atggctatca tctgtcattg ataaagaaat gcgctatctg
     7321 catgagatta tgcaaggagc tccagtatca gaagaatttg caaaactttt gacaggtgaa
     7381 gctgccctag aagcaattgc tactgcagat gcttgtaccc agtctatgtt tgaagatcgc
     7441 aaagtaaaat tgtcagaaat tgtaaaataa attttggtat tctcctatta taggtcgact
     7501 tgctcctctg aaagtacttt tagaggagct gtttgacttt gctagttttt gaaactgaaa
     7561 tctattatac tacaaactat tgaaagcgtt ttaattttaa ggtataataa tctcatagaa
     7621 ataaagaaaa ggaggaaaga ggatgccaca gattagcaaa gaagccttga ttgagcaaat
     7681 caaagatgga atcatcgttt cttgtcaggc tcttcctcat gaaccgcttt atacagaagc
     7741 gggaggggtg attcccttgc tggtcaaagc ggctgagcaa ggtggagcag tcggtatccg
     7801 agcaaacagt gttcgcgata tcaaggaaat taaggaagtc actaaacttc caatcattgg
     7861 gattatcaaa cgtgattatc cacctcagga acccttcatc acggctacta tgaaagaagt
     7921 tgatgaattg gcagaactgg acatcgaggt gattgctctg gattgtacca agcgtgaacg
     7981 ctacgatggt ttggaaattc aagagttcat tcgtcaggtt aaggagaaat atcctaatca
     8041 gcttttgatg gctgatacta gtatcttcga agaagggcta gcagctgtag aagcaggaat
     8101 tgactttgtc ggaacaacct tatcaggcta cacatcctac agtccaaaag tagacggtcc
     8161 agattttgaa ttgattaaga aactctgtga tgccggtgta gatgtcattg cagaaggaaa
     8221 aattcataca ccagaacaag ccaaacaaat ccttgaatat ggagtgcgag gcatcgttgt
     8281 tggtggcgcc attactagac caaaagagat tacagaacgc ttcgttgcta gtcttaaata
     8341 agatgtgagg gggagtttta tgtttaaagt tttacaaaaa gttggaaaag cttttatgtt
     8401 acctatagct atacttcctg cagcaggtct acttttgggg attggtggtg cactttcaaa
     8461 cccaaccacg atagcaactt atccaatact agacaatagt atttttcaat caatattcca
     8521 agtaatgagc tctgcaggag aggttgtatt cagtaatttg tcactacttc tctgtgtggg
     8581 attatgtatt ggcttagcga aacgagataa aggaaccgct gcgttagcag gagtaactgg
     8641 ttacttagtt atgactgcaa cgatcaaagc tttggtaaaa ctttttatgg cagaaggatc
     8701 tgcaattgat actggagtta ttggagcatt agttgtcgga atagttgccg tatatttgca
     8761 caaccgatat aacaatattc aattaccttc cgctttagga ttctttggag gttcacgctt
     8821 cgttcctatt gttacatcgt tctcttctat cttgattggc tttgtcttct ttgttatttg
     8881 gccacctttc caacaacttc ttgtttctac aggtggatat atttctcagg cgggtccaat
     8941 tggaactttt ctatatggat ttttaatgag actttctgga gcagtaggct tacatcatat
     9001 aatttaccct atgttttggt atactgaact tggtggtgtt gaaactgttg caggacaaac
     9061 agtggttgga gctcaaaaaa tattttttgc tcaattagcc gatttggccc attctggatt
     9121 atttacagaa ggaacaaggt tttttgcagg tcgtttctca acaatgatgt tcggtttacc
     9181 ggctgcctgt ttagcgatgt accatagtgt tcctaaaaat cgtcgtaaaa aatacgcggg
     9241 tttgtttttg ggagttgctt taacatcttt tattaccggt attacagaac caattgaatt
     9301 tatgtttcta ttcgtcagtc cggttctata tgttgttcac gcattccttg atggtgttag
     9361 cttctttatt gcagacgtct taaatatttc aataggaaac acattttcag gaggtgtaat
     9421 cgatttcaca ttatttggaa ttttgcaggg gaacgctaag acgaattggg ttcttcagat
     9481 tccatttgga cttatttgga gtgttttgta ttatattatt tttagatggt tcattactca
     9541 attcaacgtt ctaacgccag gacgaggaga agaagtagat tctaaagaaa tttctgaatc
     9601 cgcagattcg acttcaaata ctgcagatta tttaaaacag gatagcctac aaattatcag
     9661 agccttgggt ggatcaaata atatagaaga tgtagatgct tgtgtgacac gtttacgtgt
     9721 agctgtaaaa gaagttaatc aagttgataa agcactttta aaacaaattg gtgcagttga
     9781 tgtcttagaa gtgaagggtg gcattcaagc aatctatgga gcaaaagcaa tcttatataa
     9841 aaatagtatt aatgaaattt taggtgtaga tgattaagta cttactgact taataaaaaa
     9901 tagaggagag tgatggatga gtaggatgaa atgaaatcgc atacaagaaa taaagaactc
     9961 attatccaag ttggatacgc ttattacata ggagaataca aatgaaattt agaaaattag
    10021 cttgtacagt acttgcgggt gctgcggttc ttggtcttgc tgcttgtggc aattctggcg
    10081 gaagtaaaga tgctgccaaa tcaggtggtg acggtgccaa aacagaaatc acttggtggg
    10141 cattcccagt atttacccaa gaaaaaactg gtgacggtgt tggaacttat gaaaaatcaa
    10201 tcatcgaagc gtttgaaaaa gcaaacccag atataaaagt gaaattggaa accatcgact
    10261 tcaagtcagg tcctgaaaaa atcacaacag ccatcgaagc aggaacagct ccagacgtac
    10321 tctttgatgc accaggacgt atcatccaat acggtaaaaa cggtaaattg gctgagttga
    10381 atgacctctt cacagatgaa tttgttaaag atgtcaacaa tgaaaacatc gtacaagcaa
    10441 gtaaagctgg agacaaggct tatatgtatc cgattagttc tgccccattc tacatggcaa
    10501 tgaacaagaa aatgttagaa gatgctggag tagcaaacct tgtaaaagaa ggttggacaa
    10561 ctgatgattt tgaaaaagta ttgaaagcac ttaaagacaa gggttacaca ccaggttcat
    10621 tgttcagttc tggtcaaggg ggagaccaag gaacacgtgc ctttatctct aacctttata
    10681 gcggttctgt aacagatgaa aaagttagca aatatacaac tgatgatcct aaattcgtca
    10741 aaggtcttga aaaagcaact agctggatta aagacaattt gatcaataat ggttcacaat
    10801 ttgacggtgg ggcagatatc caaaactttg ccaacggtca aacatcttac acaatccttt
    10861 gggcaccagc tcaaaatggt atccaagcta aacttttaga agcaagtaag gtagaagtgg
    10921 tagaagtacc attcccatca gacgaaggta agccagctct tgagtacctt gtaaacgggt
    10981 ttgcagtatt caacaataaa gacgacaaga aagtcgctgc atctaagaaa ttcatccagt
    11041 ttatcgcaga tgacaaggag tggggaccta aagacgtagt tcgtacaggt gctttcccag
    11101 tccgtacttc atttggaaaa ctttatgaag acaaacgcat ggaaacaatc agcggctgga
    11161 ctcaatacta ctcaccatac tacaacacta ttgatggatt tgctgaaatg agaacacttt
    11221 ggttcccaat gttgcaatct gtatcaaatg gtgacgaaaa accagcagat gctttgaaag
    11281 ccttcactga aaaagcgaac gaaacaatca aaaaagctat gaaacaatag tccttagtta
    11341 ttctataaaa agtagttttt taaagaacct aagagtgtat accccctttt ccctctacac
    11401 agatagtgta agaaaagggg gcttttgttt aaaatgtaag aaactgtcac gaaattaaaa
    11461 tgaagttctt acataagcga atcataaaaa atttcatttt gattttaaaa cagttcaaga
    11521 aagtcaaaaa attattctat ttgaaagaga ggtgccgact gtgaaagtca ataaaatccg
    11581 tatgcgggaa acagtgattt cctacgcttt cctagcacca gtattattct tctttgtcat
    11641 ctttgtgttg gctccgatgg tgatgggctt cattacaagt ttctttaact actcaatgac
    11701 taaatttgag tttgtaggct tggataacta tatccgtatg tttaaagatc ctgtctttac
    11761 aaaatctctg attaacacag ttattttggt tattggatct gtaccagttg ttgttctatt
    11821 ctcactcttt gtagcatctc agacctatca tcaaaatgtc attgccagat ccttctaccg
    11881 tttcgtattc ttccttcctg ttgtaacggg tagtgttgcc gtgacagttg tttggaaatg
    11941 gatttatgac ccgctatcag ggattctaaa ctttgtcctt aagtcaagcc acatcatcag
    12001 ccaaaacatt tcttggttgg gagataaaaa ctgggcattg atggcgatta tgattattct
    12061 cttgaccact tcagttggtc agcccatcat cctttatatc gctgccatgg ggaatattga
    12121 caattcactg gttgaagcgg cgcgtgttga tggtgcaact gagtttcaag ttttttggaa
    12181 gattaaatgg ccaagccttc ttccaacaac tctttatatt gcaatcatca caacaattaa
    12241 ctcattccag tgtttcgcct tgattcagct tttgacatct ggtggtccaa actactcaac
    12301 aagtaccttg atgtactacc tttacgaaaa agccttccaa ttgacagaat acggctatgc
    12361 caacacaatt ggtgtcttct tggcagtcat gattgctatc gtaagctttg ttcaatttaa
    12421 agtacttgga aacgacgtag aatactaaag aaaggagaca gctatgcaat ctacagaaaa
    12481 aaaaccatta acagccttta ctgttatttc aacaatcatt ttgctcttgt tgactgtgct
    12541 gttcatcttt ccattctact ggattttgac aggggcattc aaatcacaac ctgatacaat
    12601 tgttattcct cctcagtggt tccctaaaat gccaaccatg gaaaacttcc aacaactcat
    12661 ggtgcagaac cctgccttgc aatggatgtg gaactcagta tttatctcat tggtaaccat
    12721 gttcttagtc tgtgcaacct catctctagc aggttatgta ttggctaaaa aacgtttcta
    12781 tggtcaacgc attctatttg ctatctttat cgctgctatg gcgcttccaa aacaagttgt
    12841 ccttgtacca ttggtacgta tcgtcaactt catgggaatc cacgatactc tctgggcagt
    12901 tatcttgcct ttgattggat ggccattcgg tgtcttcctc atgaaacagt tcagtgaaaa
    12961 tatccctaca gagttgcttg aatcagctaa aatcgacggt tgtggtgaga ttcgtacctt
    13021 ctggagtgta gccttcccga ttgtgaaacc agggtttgca gcccttgcaa tctttacctt
    13081 catcaatact tggaatgact acttcatgca gttggtaatg ttgacttcac gtaacaattt
    13141 gaccatctca cttggggttg cgaccatgca ggctgaaatg gcaaccaact atggtttgat
    13201 tatggcagga gctgcccttg ctgctgttcc aatcgtcaca gtcttcctag tcttccaaaa
    13261 atccttcaca cagggtatta ctatgggagc ggtcaaagga taatactctg cgaaaatcga
    13321 atgcaaacta cgtcagcttc accttgctat acttaagtat tgcctgcggt tagcttccta
    13381 gtttgttctt caattttcat tgagtatagg aaaatcaatc tatcaagata cagaagtata
    13441 ttttatagat ttagagaata tagaggttat aagtgtctac aaaatggagg gtatgcagtt
    13501 actttatgaa gttttgtcag acacttataa acttaagaat ggttttagtt aactatcaga
    13561 aacgaaggaa agagtatgat ttttgacgat ttgaaaaaca tcacctttta caaagggatt
    13621 catcccaatt tagacaaggc tatcgactac ctctaccaac atcgtaaaga ttcattcgaa
    13681 ttaggaaagt atgagattga tggagataaa gtctttctag ttgttcagga aaatgtcctc
    13741 aatcaagttg agaataatca atttgaacac cataagaact atgcagattt gcatttgctg
    13801 atagaagggc atgaatattc gagctacggt tcacgtatca aagacgaggc agtagcattc
    13861 gacgaagcga ctgacattgg ctttgttcat tgtcatgaac actacccact cttgttgggt
    13921 tatcacaatt ttgcgatttt cttcccaggt gagccacatc agccaaatgg ttatgcaagc
    13981 atggaagaaa aggttcggaa atatctcttt aaaattttga ttgattaaaa ataggatgaa
    14041 ttgttttttg taaagctttg acaatactat accatgaaat tgatctttgt gaggtgggga
    14101 aatgagaata aaatatttaa aaattggtat cttcttgtca gtctgtgtat gctacaagag
    14161 ctagtttttt ggatggacag ggaattacag ttgatgagat ggcttggata attaggggca
    14221 ttgtgaatgc attgattggt agatacataa aattaggtac ttatgcggct aagtatggta
    14281 ttagtatggc gcgttctatc ttaaataggg cagctgcaac tgcggcagca agagtaagat
    14341 tattgactaa gatttttgga tggattttac gaatagctgt gctcgcagct gatgtatatg
    14401 gtaattttgc caacaatatt gctgcagctt gggatgcaca tgataaaatc cctaacaatg
    14461 gtcgtataaa cttttaaaat gcgagaatga aagcactttg tattttttta ttgtatatgt
    14521 tagcttggac agtgcttgca atgataatcc gtggagggct agatggattt gatgaacaca
    14581 cttggcgtaa tattttagct agtgcgttgt cctcgctata catacattat atatccatag
    14641 atagaaaaag aaaaaagtcc aaaagaaaaa agaaaaaata gatttgttca tggtagggac
    14701 ttatgaaagc tttactaaca aaaaagaaaa cagtttacaa agaaaaatga tggaggagta
    14761 aacatggcac aaaaaggagt aagccttatc aaggcagcat ttgatacaga taactttctt
    14821 atgcgtttta gtgagaaagt cttggacatc gtgacagcca atcttctttt tgtcgtctct
    14881 tgtttgccca tcgtgacgat tggagtggct aaaatcagcc tctacgagac catgtttgag
    14941 attaagaaga gcagacgggt gcctgttttt aaaatctatc taagatcttt caagcaaaat
    15001 ctgaaactag gtcttcagtt aggtctgctt gagttgggca ttgtttcatt aagcctccta
    15061 gatctttatc tcttttgggg tcaaacagct ctgccctttc aattgctgaa agctatttgt
    15121 ctaggcattc tgattttcct tacgattgtg atgttggcta gctatccaat cgcggcacgt
    15181 tatgatttaa cttggaaaga agtgttacaa aaagggctta tcttggcaag ttttaacttt
    15241 ccttggttct tcctcatgtt agccattctt gtcctcattg tgatggttct ttatctgtcc
    15301 gccttcagtc tactcttagg tggctcagtc ttcctacttt ttgggtttgg actattggtc
    15361 tttatccaga ctggattgat ggagaaaatt ttcgcaaaat accaatagga gccttatttc
    15421 tgaaactact ttcaaaggct ccaaacgcta ttctataagc gagaaactaa aatctaagtg
    15481 tctataagat attgaaagcc ttttcggtaa agtgtatact aaagaagtaa agaaaaatct
    15541 gctttagcag aaaataaaaa taataggaga aaatctatgt cagatttgaa aaaatacgaa
    15601 ggtgtcattc cagccttcta cgcatgttat gatgatcaag gagaagtaag cccagaacgt
    15661 acgcgtgcct tggttcaata cttcattgat aaaggtgttc aaggtcttta tgtcaatggt
    15721 tcttctggtg aatgtatcta ccaaagcgtt gaagatcgca agttgatttt ggaagaagtc
    15781 atggcggtag ctaaaggtaa attgaccatt attgcccatg ttgcctgcaa caatactaaa
    15841 gacagtatgg aacttgctcg ccatgctgaa agcttgggag tagatgctat tgcaacgatt
    15901 ccaccaattt atttccgctt gccagaatac tcagttgcca aatactggaa cgatatcagt
    15961 tctgcagctc caaacacaga ctacgtgatt tacaacattc ctcaattggc aggggttgct
    16021 ttgactccaa gcctttacac agaaatgttg aaaaatcctc gtgttatcgg tgttaagaac
    16081 tcttctatgc cagttcaaga tatccaaacc tttgtcagcc ttggcgggga agaccatatt
    16141 gtctttaacg gtccagatga acagttccta ggtggccgcc tcatgggtgc taaggctggt
    16201 atcggtggta cttatggtgc tatgccagaa ctcttcttga aactcaatca gttgattgcg
    16261 gataaggacc tagaaacagc gcgtgaattg cagtatgcta tcaacgcaat cattggtaaa
    16321 ctcacttctg ctcatggaaa tatgtacggt gtcatcaaag aagtcttgaa aatcaatgaa
    16381 ggcttgaata ttggatctgt tcgttcacca ttgacaccag taactgaaga agatcgtcca
    16441 gttgtagaag cggctgcagc cttgattcgt gaaaccaagg agcgcttcct ctaatctaaa
    16501 aggaggtatt tatgacatac tacgttgcaa ttgatatcgg tggaaccaac atcaagtatg
    16561 gtttggttga tcaagagggg caacttcttg aatcgcatga aatgccaact gaggcgcata
    16621 agggtggacc tcatatctta caaaagacca aagatatcgt agctagttat ttagaaaaag
    16681 gcccagtagc aggtgttgcc atatcttctg ctgggatggt ggatccggat aagggtgaga
    16741 ttttctatgc tgggccgcaa atccctaact acgcaggcac ccagttcaaa aaggaaatcg
    16801 aagaaagctt tactattcct tgtgagattg aaaatgatgt caactgtgca ggtcttgctg
    16861 aggcagtatc tggttcaggc aagggagcaa gtgtgacact ttgcttgacc attggaacag
    16921 gtatcggtgg ttgcttgatt atggatagga aagtcttcca tggttttagc aattcagcct
    16981 gtgaagtcgg gtatatgcat atgcaggatg gagcttttca agacttggct tctacaacag
    17041 ctttagtgaa atatgtagct gaagcccatg gagaagatgt tgatcagtgg aatggccgta
    17101 gaattttcaa agaagccact gaaggaaaca aaatctgcat ggaaggtatt gaccgtatgg
    17161 ttgactatct aggaaaaggt ctggcaaata tttgctacgt tgccaatcca gaagtggtca
    17221 ttcttggtgg tggtatcatg gggcaagagg ctatcctcaa acctaagatc cgcacagcct
    17281 tgaaagaggc tttggtacca agtttagcag aaaaaacacg attagaattt gcccatcacc
    17341 aaaatacagc agggatgttg ggtgcatatt atcattttaa gacaaaacaa tcctagtttg
    17401 gctcagccaa actaggattt tcttacacgt ttttgtctac gatagccgtt gagtttttta
    17461 ttttcccagt agctattaaa gattttttcc ttgctttcgc gattgatttc caaaaagtag
    17521 gcataaatca aatcgataaa gaagagcata ggaagttgag cggatattcg ttggatatag
    17581 gagggttggc tgtgggtggc tacaagaaca gtctctgtat aggtctggct atttttattg
    17641 ggaacacttg taaagagtac agtctttgcc cccatctcct tggcatctaa tagactatct
    17701 aaaatagaag gagttgagcc tgaaagtgag aagcccagta ctagacaatt ttcatccatg
    17761 atgctggttg tccaggcaaa gccgtcttgg tctgtcaaag cttcgcagac cacacctaat
    17821 cgcataaaac gtaatttcat ttcacgggcg acgaggccag aactccctgt tccaaagaag
    17881 tagatacgct cagcatcttc gattagctgg gcaattcgtt ctagttggat ttcgtcaatc
    17941 aagtcttgtg tttgttccct catattgcta taacttctga ggactcgttt ggtcagtgga
    18001 ctgtgcttgg aaacttggtt ggcttgattt tctgcctgat gttggtattg gaaaataaat
    18061 tctcggtagc cagtaaagcc acacttttta gcaaagcggg tcaaagcagc ttgagaaata
    18121 tgtaattttt gggtgacttg ttgagaagat aaatcatctg taatcgtttc agcttgcaaa
    18181 aaatagcgag cgatttcttg ctctaggtct gtcatttctt caaaatgtga atcaatgata
    18241 gttgcgatat ctggtttgtc catagggaaa gctcctttac atgagtcata ctggaagact
    18301 agatcaggga atagtcacac ttcattataa cacataatat aaggatagat aaataaaaac
    18361 gcatctctgt tttaaaaacg aaaaaatcga aaaagcttct ctcttttcca taattttcta
    18421 ctcaaattgt ggtacaatta agagtaagat tttaagttag aaatgagact gatttgtatg
    18481 agaaaattta acagccattc gattccgatt cggcttaatt tattgttttc aatcgtcatt
    18541 ttactcttta tgaccattat tggtcgtttg ttgtatatgc aggttttgaa caaggatttt
    18601 tacgaaaaaa agctagcctc agctagtcag accaagatta caagcagttc agcccgtggg
    18661 gaaatttatg atgctagtgg aaaacctttg gtagaaaata cgttaaagca ggttgtttcc
    18721 tttacgcgta gcaataaaat gacggctaca gacttaaaag aaacagctaa aaagttactg
    18781 acttatgtga gcatcagttc tccaaatttg acagaacgcc agctggcgga ttactatttg
    18841 gctgatcctg aaatctataa aaaaatagtg gaagctctcc caagcgagaa acgcttggat
    18901 tcagatggca atcgtctatc cgaatcagaa ctgtataaca atgcggtcga tagtgtacaa
    18961 acgagtcaac taaactatac agaggatgaa aagaaagaaa tctatctttt tagtcagtta
    19021 aatgctgttg gaaactttgc gacaggaacc attgcgacag atcctctaaa tgattctcag
    19081 gtggctgtta ttgcctctat ttcaaaggag atgcctggca ttagtatttc tacttcttgg
    19141 gatagaaagg ttttggaaac ttccctttct tctatagttg ggagtgtatc cagtgaaaaa
    19201 gctggtctcc cagcggaaga agcagaagcc tatcttaaaa aaggctattc tctaaatgac
    19261 cgtgtaggaa cctcctattt ggaaaagcaa tatgaagaga ccttacaagg aaaacgctcg
    19321 gtaaaagaaa tccatctgga taaatatggc aatatggaaa gcgtggatac aattgaggaa
    19381 ggtagtaagg gaaacaatat caaactgacc attgatttgg ctttccaaga tagcgtggat
    19441 gctttactga aaagttattt caattctgag ctagaaaatg gtggagccaa gtattctgaa
    19501 ggtgtctatg cagtcgccct taacccaaaa acaggtgcgg ttttgtctat gtcagggatt
    19561 aaacatgact tgaaaacggg agagttgacg cctgattcct tgggaacggt aaccaatgtc
    19621 tttgttccag gttcggttgt caaggcggcg accatcagct caggttggga aaatggagtc
    19681 ttgtcaggaa accagacctt gacagaccag tccattgtct tccaaggttc agctcccatc
    19741 aattcttggt atactcaggc ttacggttca ttccctatca cagcggtcca agctctggag
    19801 tattcatcaa atacctatat ggtccaaaca gccttaggtc ttatggggca aacctatcaa
    19861 cccaatatgt ttgtcggcac cagcaatcta gagtctgcta tggagaaact gcgttcaacc
    19921 tttggcgaat atggcttggg tactgcgaca ggaattgacc taccagatga atctactgga
    19981 tttgttccca aagagtatag ctttgctaat tacattacta atgcctttgg gcagtttgat
    20041 aactatacgc cgatgcagtt ggctcagtat gtagcaacta ttgcaaataa tggtgttcgt
    20101 gtggctcctc gtattgttga aggcatttat ggtaataatg ataagggagg actgggtgac
    20161 ttgattcagc aactgcaacc gacagagatg aataaggtca atatatccga ctccgatatg
    20221 agcatcttgc accaaggttt ttatcaggtt gcccatggta ctagtggatt gacaactgga
    20281 cgtgcctttt caaatggtgc cttggtatcc attagcggaa aaacaggtac agccgaaagc
    20341 tatgtggcag atggtcagca agcaaccaat accaatgcgg tggcctatgc cccatctgat
    20401 aatccccaaa tcgctgtcgc agtggtcttt cctcataata ccaatctaac aaatggtgta
    20461 ggaccttcca ttgcgcgtga cattatcaat ctgtatcaaa aataccatcc aatgaactag
    20521 aaaggaaatt atgctttatc caacacctat tgccaagttg attgacagtt attctaagtt
    20581 accaggtatc gggattaaga cggctacgcg tctggccttt tatacgattg ggatgtctgc
    20641 tgatgatgtc aatgaatttg caaaaaatct cctttctgct aagagagaat tgacatattg
    20701 ttctatttgt ggacgtttga cagacgacga tccttgttct atctgtactg atccgactcg
    20761 tgaccagaca acaattttag ttcttgagga tagtagagat gtggcagcca tggaaaatat
    20821 ccaagaatac catggactct atcatgtcct tcatggcctc atttctccta tgaatggtat
    20881 cagtccggac gatatcaatc tcaagagtct tatgactcgt cttatggata gtgaggtttc
    20941 agaagtgatt gtggcgacta atgctacagc ggatggtgaa gcgacttcca tgtatctttc
    21001 acgtttgctc aagccggctg gtatcaaggt tacgcgtcta gcacgaggtc tcgctgtggg
    21061 agcggacatt gagtatgcgg acgaagtgac actcttacga gcgattgaaa atcggacaga
    21121 gttgtaagtg taggcaaatt tacgaactcc attcatttat aaaaaatcaa agaggctgaa
    21181 aatcgttcct atcggcctct ttttgtatag tgtgatgagt aggatcaggt tcaagtttta
    21241 aaaaaccaag caaatatgat atactaaaga gcgagtattc tagtaggatt aggacaaata
    21301 atatgaaaca aacgattatt cttttatatg gtggacggag tgcggaacgc gaagtctctg
    21361 tcctttcagc tgagagtgtc atgcgtgcgg tcgattacga ccgtttcaca gtcaagactt
    21421 tctttatcag tcagtcaggt gactttatca aaacacagga atttagtcat gctccggggc
    21481 aagaagaccg tctcatgacc aatgaaacca ttgattggga taagaaagtt gcaccaagtg
    21541 ctatctacga agaaggtgca gtggtctttc cagtccttca cgggccagtg ggagaagatg
    21601 gctctgttca aggattcttg gaagttttga aaatgcctta cgttggttgc aacattttgt
    21661 catcaagtct tgccatggat aaaatcacga ctaagcgtgt tctggaatct gctggtattg
    21721 cccaagttcc ttatgtggct atcgttgaag gcgatgatgt gactgctaaa atcgctgaag
    21781 tggaagaaaa attggcttat ccagtcttca ctaagccgtc aaacatgggg tctagtgtcg
    21841 gtatttctaa gtctgaaaac caagaagaac tacgtcaagc cttaaaactt gccttccgat
    21901 atgacagccg tgtcttggtt gagcaaggag tgaatgcccg tgaaattgag gttggcctct
    21961 tgggtaacta cgatgtcaag agcacgctgc caggagaagt tgtcaaggac gttgcctttt
    22021 atgactacga tgccaagtat attgataaca aggttactat ggatattcct gccaaaatca
    22081 gtgatgatgt ggtggctgtc atgcgtcaaa atgcagaaac agccttccgt gccattggtg
    22141 gccttggtct atctcgttgc gatttcttct atacagataa gggagagatt tttctcaacg
    22201 agctcaatac catgccaggt ttcacccagt ggtctatgta cccactactt tgggacaatg
    22261 tggggatcag ctacccagaa ctaatcgagc gtttggttga ccttgccaag gaaagttttg
    22321 acaagcgcga agcgcatttg atataaaatg aaagagaggg tagaagccag aaccatcact
    22381 gcaaggtgac tagagttctc ggacttcagc cctttttaaa ggagtagaaa tgaaattaac
    22441 aatccatgaa attgcccaag ttgttggagc caaaaatgat atcagtatct ttgaggacac
    22501 ccagttagaa aaagctgagt ttgatagtcg tttgattgga actggagatt tatttgtgcc
    22561 acttaaaggt gcgcgtgatg gccatgactt tattgaaaca gcctttgaaa atggtgcagc
    22621 agtaaccttg tctgagaaag aggtctcaaa tcatccttac attctagtag atgatgtttt
    22681 gacagccttt caatccctag catcctacta tcttgaaaaa acgactgttg atgtctttgc
    22741 tgttacaggt tcaaatggca agacaacgac taaggatatg ttggcgcatt tactgtcaac
    22801 gacctacaag acctacaaaa cacaaggcaa ctacaataac gagattggcc ttccctacac
    22861 agttctacac atgcctgaag gaacagaaaa gttggttttg gagatggggc aggaccactt
    22921 gggcgatatt catctcttgt ctgaattggc tcgtccaaaa acagccatcg tgaccttggt
    22981 tggagaagcc catttggcct ttttcaaaga ccgttcagag attgctaagg gaaaaatgca
    23041 aattgcagac ggaatggctt caggttcctt gattttagcg ccggctgacc ctatcgtaga
    23101 ggactatttg ccaactgata aaaaggtggt tcgttttggg caaggggcag agctggaaat
    23161 tactgacttg gttgagcgca aagatagtct gaccttcaag gccaatttct tagagcaagc
    23221 ccttgatttg ccagtaactg gcaagtacaa tgcgacaaat gctatgattg cgtcctatgt
    23281 tgccctacaa gaaggagttt cagaggagca aattcatcag gccttccaag gtcttgaatt
    23341 gacgcgtaat cgtactgagt ggaagaaagc agccaatgga gcagatatcc tgtcagacgt
    23401 atataatgcc aatccaactg ctatgaagct gattttagag actttctctg ccattccagc
    23461 caatgaaggt ggcaagaaaa ttgcagtgtt ggcggatatg aaggagcttg gtgaccagtc
    23521 tattcaactc cataaccaga tgattttgag cctttctcca gatgtgcttg ataccgttat
    23581 tttctatgga gaagacattg ctgaattagc ccaacttgct agtcaaatgt tcccaatcgg
    23641 acacgtttat tacttcaaga aaacagaaga ccaagaccaa tttgaagacc tagttaagca
    23701 ggtcaaagaa agccttgggg ctcatgacca aatcctgctc aaaggctcta actctatgaa
    23761 tctagccaag ttggtagaaa gtttagaaaa tgaagacaag tgattttgtc aaacatttac
    23821 gtatttctaa atccattttt gacaactagc atggtataat aatatgcagg aaaattttga
    23881 attatgagga agactagatg aatttatggg atattttctt tacgactcag gcaaccgagc
    23941 cgcccaaatt tgaccttttt tggtatgtta gcctatttac gctcttagcc ttaacctttt
    24001 atacagccca tcgctatcgt gaaaagaagg tttaccaacg atttttccaa atcttgcaga
    24061 ctgttcagtt aatccttctt tatggttggt actgggttaa tcatatgcca ctgtcagaaa
    24121 gcctaccctt ttaccattgc cgtatggcta tgtttgtggt actcttgctt cctggtcagt
    24181 ccaaatataa acaatacttt gcattattgg gaacatttgg gacattagca gcctttgttt
    24241 atccagtgcc agatgcttac ccttttccac atattaccat tttatccttt atctttggtc
    24301 atttagcact cttggggaac tctctagttt atctattgag acagtataat gcgcgattgc
    24361 tggatgtgaa gggaattttt ctcatgacct ttgccctaaa tgccttgatt tttgtggtca
    24421 atttggtgac aggtggcgat tacggatttt tgacaaaacc gccattggtt ggggatcacg
    24481 gtctagtagc taattattta cttgtttcaa ttgtgctggt agctactatc agtttgacta
    24541 agaaaatctt agaattcttt ttagctcaag aagcagaaaa aatgattgca aaggaagctt
    24601 aacacagagc tttctttttt gctcttagag agtttttaca agcagcttat aaaataagaa
    24661 tttctgaata gacaaactca aaaaatggct gggaaattta ggaaaaaagc aagcacgatt
    24721 aaattttttg tgttataata ttttgtgaat agctatgcct atgtttagct atggaataat
    24781 acgaagtgcg aaacttggaa gatagagagg aagcgatgta atggctagag aaggcttttt
    24841 tacaggtcta gatattggaa caagctctgt caaggtgctt gtggccgagc agagaaatgg
    24901 tgaattaaat gtaattggcg tgagtaatgc caaaagtaaa ggtgtaaagg atggaattat
    24961 tgttgatatt gatacagcag caactgctat caagtcagcc atttcccaag cggaagaaaa
    25021 ggcaggcatt tcgattaaat cagtgaatgt cggcttgcct ggtaatcttt tgcaggtaga
    25081 accaactcag gggatgattc cagtaacatc tgatactaag gaaattacgg atcaagatgt
    25141 tgaaaatgtt gtcaaatcag ctttgacaaa gagtatgaca cctgaccgtg aagtcattac
    25201 ctttattcct gaagaattta ttgtggatgg tttccaaggg attcgtgacc cacgtggcat
    25261 gatgggggtt cgccttgaaa tgcgtggttt gctttataca ggacctcgta ctatcttgca
    25321 caatttgcgt aagacggttg agcgtgcagg tgttcaggtt gaaaatgtta tcatttcacc
    25381 actagcaatg gttcagtctg ttttgaacga aggggaacgt gaatttggtg ctacagtgat
    25441 tgatatgggg gcaggtcaaa cgactgtcgc tacaatccgt aatcaagaac tccagttcac
    25501 acatattctc caagaaggtg gagattatgt aactaaagat atctccaagg ttttgaaaac
    25561 ctctcgcaaa ttagcggaag gcttgaaact gaattacggg gaagcctatc cgcctcttgc
    25621 aagcaaagaa accttccaag tagaggttat tggagaagta gaagcagtcg aagtgacgga
    25681 agcctacttg tcagaaatta tttctgcacg aatcaagcac atccttgaac aaatcaagca
    25741 agaattagat agaaggcgtc tattggacct ccctggtggt attgtcttaa tcggtgggaa
    25801 tgccatttta ccaggtatgg ttgagcttgc tcaggaagtc tttggcgtcc gtgtcaagct
    25861 ttatgttcca aatcaagttg gtatccgtaa tccagccttt gcgcatgtga ttagtttatc
    25921 agaatttgcg ggtcaattaa cagaagttaa tcttttggct cagggagcga taaaaggtga
    25981 gaatgactta agtcatcagc caattagttt tggtgggatg ctgcaaaaaa cagctcagtt
    26041 tgtacaatca acgcctgttc aaccagctcc tgctccagaa gtagagccgg tggcgcctac
    26101 agaaccaatg gcggatttcc aacaagcttc acaaaataaa ccgaaattag cagatcgttt
    26161 ccgtggcttg atcggaagca tgtttgacga ataaagagga aaaataaatt atgacatttt
    26221 catttgatac agctgctgct caaggggcag tgattaaagt aattggtgtc ggtggaggtg
    26281 gtggcaatgc catcaaccgt atggtcgacg aaggtgttac aggcgtagaa tttatcgcag
    26341 caaacacaga tgtacaagca ttgagtagta caaaagctga gactgttatt cagttgggac
    26401 ctaaattgac tcgtggtttg ggtgcaggag gtcaacctga ggttggtcgt aaagccgctg
    26461 aagaaagcga agaaacactg acggaagcta ttagtggtgc cgatatggtc ttcatcactg
    26521 ctggtatggg aggaggctct ggaactggag ctgctcctgt tattgctcgt atcgccaaag
    26581 atttaggtgc gcttacagtt ggtgttgtaa cacgtccctt tggttttgaa ggaagtaagc
    26641 gtggacaatt tgctgtagaa ggaatcaatc aacttcgtga gcatgtagac actctattga
    26701 ttatctcaaa caacaatttg cttgaaattg ttgataagag aacaccgctt ttggaggctc
    26761 ttagcgaagc ggataacgtt cttcgtcaag gtgttcaagg gattaccgat ttgattacca
    26821 atccaggatt gattaacctt gactttgccg atgtgaaaac ggtaatggca aacaaaggga
    26881 atgctcttat gggtattggt atcggtagtg gagaagaacg tgtggtagaa gcggcacgta
    26941 aggcaatcta ttcaccactt cttgaaacaa ctattgacgg tgctgaggat gttatcgtca
    27001 acgttactgg tggtcttgac ttaaccttga ttgaggcaga agaggcttca caaattgtga
    27061 accaggcagc aggtcaagga gtgaacatct ggctcggtac ttcaattgat gaaagtatgc
    27121 gtgatgaaat tcgtgtaaca gttgtcgcaa cgggtgttcg tcaagaccgc gtagaaaagg
    27181 ttgtggctcc acaagctaga tctgctacta actaccgtga gacagtgaaa ccagctcatt
    27241 cacatggctt tgatcgtcat tttgatatgg cagaaacagc tgaattgcca aaacaaaatc
    27301 cacgtcgttt ggaaccaact caggcatctg cttttggtga ttgggatctt cgccgtgaat
    27361 cgattgttcg tacaacagat tcagtcgttt ctccagtcga gcgctttgaa gccccaattt
    27421 cacaagatga agatgaattg gatacacctc catttttcaa aaatcgttaa gtaaatgaat
    27481 gtaaaagaaa atacagaact tgtttttcga gaagttgcag aggctagtct gagtgctcat
    27541 cgagagagtg gttcggtctc tgtcattgca gttaccaagt atgtagatgt accgacagcg
    27601 gaagccttgc ttccgctagg tgttcatcat atcggtgaaa atcgtgtaga taagtttctg
    27661 gaaaaatatg aagctttaaa agatcgagat gtgacttggc atttgattgg taccttgcaa
    27721 agacgtaagg tgaaagatgt cattcaatac gttgattatt tccatgcatt ggactcagta
    27781 aagctagcag gggaaattca aaaaagaagt gaccgagtca tcaagtgttt ccttcaagta
    27841 aatatttcta aagaagaaag caaacacggt ttttcgagag aggaactgct ggaaatcttg
    27901 ccagagttag ccagactaga taagattgaa tatgttggtt taatgacgat ggcacctttt
    27961 gaggctagca gtgagcagtt gaaagagatt ttcaaggcga cccaagattt acaaagagaa
    28021 attcaagaga aacaaattcc aaatatgcct atgaccgagt taagtatggg aatgagtcgt
    28081 gattataaag aagcgattca attcggttcc acttttgttc gtataggtac atcatttttt
    28141 aagtaggaga gaaccatgtc tttaaaagat agattcgata gatttataga ttattttacg
    28201 gaggatgagg attcaagtct cccttatgaa aaaagagatg agcctgtgtt tactccagta
    28261 aattcttcac aggaaccggc tctcccaatg aatcaacctt cacagtcggc tggtacaaaa
    28321 gagaacaata tcaccagact tcatgcaaga caacaggaat tggcaaatca gagtcagcgt
    28381 gcaacggata aggtcattat agatgttcgt tatcctagaa aatatgagga tgcaacagaa
    28441 attgttgatt tattggcagg aaacgaaagt atcttgattg attttcagta tatgacagag
    28501 gtgcaggctc gtcgttgttt ggactatttg gatggagctt gtcatgtttt agctggaaat
    28561 ttgaaaaagg tagcttctac catgtatttg ttgacaccag tgaatgttat tgtaaatgtt
    28621 gaagatatcc gtttaccaga tgaagatcaa cagggtgagt tcggttttga tatgaagcga
    28681 aatagagtac gataatgatt tttttaattc gtatgattta taatgcagtg gatatttact
    28741 ccctaatttt ggtagccttc gctgtcatgt cttggtttcc aggtgcctac gaatccagtt
    28801 taggtcgttg gattgtagcg ttggtaaaac cagtgcttgc tcccttgcaa cgcctgcctt
    28861 tacagatagc gggtcttgat ttatctgttt gggtcgcgat tgttttggtt cgatttttag
    28921 gagaaaacct agtgcgtttt ctggcgatga taggatgaat aaagggattt atcagcattt
    28981 ctccatagaa gatcgtccat ttcttgacaa gggaatggaa tggataaaga aggtagaaga
    29041 tagctatgct ccttttttaa ctccttttat caatcctcat caggagaagc tattaaagat
    29101 tttggccaaa acctatggtc ttgcttgtag cagtagtggg gaattcgtct cgagtgagta
    29161 tgttcgagtt ttattatacc cagattattt ccaaccagag ttttcagatt ttgaaatatc
    29221 tctccaagaa attgtgtatt ccaataaatt tgaacattta acgcatgcta agattttagg
    29281 gacagtcatc aatcaattag ggattgaacg gaaacttttt ggagatatcc tagtagatga
    29341 agaacgggcg cagattatga ttaatcagca gtttcttctt ctctttcaag atggactaaa
    29401 gaaaattggt cgtatacctg tttcgctgga ggaacgtcct ttcaccgaga aaatagataa
    29461 gctagaacag tatcgagaac tgaatttatc tgtgtctagt tttcgattag atgttctttt
    29521 atcaaatgtt ttgaaactat ctaggaatca agcaaaccag ttgattgaaa agaaacttgt
    29581 ccaagtaaat tatcatgtgg tagacaaatc agattacact gttcaagttg gagacttgat
    29641 tagtgtgaga aaatttggtc gcttgagatt atttcaagat aagggacaaa cgaaaaaaga
    29701 gaagaaaaaa ataaccgtcc agttattatt aagtaagtga ggaatagaat gccaattaca
    29761 tcattagaaa taaaggacaa gacctttgga actcgattca gaggttttga tccagaagaa
    29821 gtcgatgaat ttttagatat tgtggttcgt gattacgaag atcttgtgcg tgcgaatcat
    29881 gataaaaatt tgcgtattaa gagtttagaa gagcgtttgt cttactttga tgaaataaaa
    29941 gattcattga gccagtctgt attgattgcc caggatacag ctgagagagt gaaacaggcg
    30001 gcgcatgaac gttcaaacaa tatcattcat caagcagagc aagatgcgca acgcttgttg
    30061 gaagaagcta aatataaggc aaacgagatt cttcgtcaag caactgataa tgctaagaaa
    30121 gtcgctgttg aaacagaaga attgaagaac aagagccgtg tcttccacca acgtctcaaa
    30181 tctacaattg agagtcagtt ggctattgtt gaatcttcag attgggaaga tattctccgt
    30241 ccaacagcta cttatcttca aactagtgat gaagccttta aagaagtggt tagcgaagta
    30301 cttggagaac cgattccagc tccaattgaa gaagaaccaa ttgatatgac acgtcagttc
    30361 tctcaagcag aaatggaaga attacaagct cgtattgagg tagccgataa agaattgtct
    30421 gaatttgaag ctcagattaa acaggaagtg gaaactccaa ctcctgtagt gagtcctcaa
    30481 gttgaagaag agcctctgct catccagttg gcccaatgta tgaagaacca gaagtagctc
    30541 cagtgcatcc gacaggtcca acaccagcta cagaaactgt tgattcagca ccgggatttg
    30601 aagcaccgca agaatctgtt acaattttat aagaaatatt ctgagaacaa tatcttatcc
    30661 ttatattccc agcgagcagg agatggtgtg agtcctgcat tccctatcga taagattatc
    30721 ctctcaaact atcaagtctg aatctagtaa gatttgacgt tccccacgtt acgggataag
    30781 agggagaaag actaaaactt tttccgaata aaggtggtac cacgattttc gtcctttttg
    30841 gaagtcgtgg tttttaattt gttattattt ataaaggaga taccatgaaa ctcaaagaca
    30901 cccttaatct tgggaaaact gaattcccaa tgcgtgcagg ccttcctacc aaagagccag
    30961 tttggcaaaa ggaatgggaa gatgcaaaac tttatcaacg tcgtcaagaa ttgaaccaag
    31021 gaaaacctca tttcaccttg catgatggcc ctccatacgc taacggaaat atccacgttg
    31081 gacatgctat gaacaagatt tcaaaagata tcattgttcg ttctaagtct atgtcaggat
    31141 tttacgcacc atttattcct ggttgggata ctcatggtct gccaatcgag caagtattgt
    31201 caaaacaagg tgtcaaacgt aaagaaatgg acttggttga gtacttgaaa ctttgccgtg
    31261 agtacgctct ttctcaagta gataaacaac gtgaagattt taaacgtttg ggtgtttctg
    31321 gtgactggga aaatccatat gtgaccttga ctcctgacta tgaagcagct caaattcgtg
    31381 tatttggtga gatggctaat aagggttata tctaccgtgg tgccaagcca gtttactggt
    31441 catggtcatc tgagtcagcc cttgctgaag cagagattga ataccatgac ttggtttcaa
    31501 cttcccttta ctatgccaac aaggtaaaag atggcaaagg agttctagat acagatactt
    31561 atatcgttgt ctggacaacg actccattta ccatcacagc ttctcgtggt ttgacggttg
    31621 gtgcagatat tgattacgtt ttggttcaac ctgctggtga agctcgtaag tttgtcgttg
    31681 ctgctgaatt attgactagc ttgtctgaga aatttggctg ggctgatgtt caagttttgg
    31741 aaacttaccg tggccaagaa ctcaaccaca tcgtaacaga acacccatgg gatacagctg
    31801 tagaagagtt ggtaattctt ggtgaccacg ttacgactga ctctggtaca ggtattgtcc
    31861 atacagcccc tggttttggt gaggacgatt acaatgttgg tattgctaat aatcttgaag
    31921 tcgcagtgac tgttgatgaa cgtggtatca tgatgaagaa tgctggtcct gaatttgaag
    31981 gtcaattcta tgaaaaggta gttccaactg ttattgaaaa acttggtaac ctccttctta
    32041 cccaagaaga aatctctcac tcatatccat ttgactggcg tactaagaaa ccaatcatct
    32101 ggcgtgcagt tccacaatgg tttgcctcag tttctaaatt ccgtcaagaa atcttggacg
    32161 aaattgaaaa agtgaaattc cactcagaat ggggtaaagt ccgtctttac aatatgatcc
    32221 gtgaccgtgg tgactgggtt atctctcgtc aacgtgcttg gggtgttcca cttcctatct
    32281 tctacgctga agatggtaca gctatcatgg tagctgaaac tattgaacac gtagctcaac
    32341 tttttgaaga acatggttca agcatttggt gggaacgtga tgccaaagac ctcttgccag
    32401 aaggatttac tcatccaggt tcaccaaacg gcgagttcaa aaaagaaact gatatcatgg
    32461 acgtttggtt tgactcaggt tcatcatgga atggagtggt ggtaaaccgt cctgaattga
    32521 cttacccagc cgacctttac ctagaaggtt ctgaccaata tcgtggttgg tttaactcat
    32581 cacttatcac atctgttgcc aaccatggcg tagcacctta caaacaaatc ttgtcacaag
    32641 gttttgccct tgatggtaaa ggtgagaaga tgtctaaatc tcttggaaat actattgctc
    32701 caagcgatgt tgaaaaacaa ttcggtgctg aaatcttgcg tctctgggta acaagtgttg
    32761 actcaagcaa tgacgtgcgt atctctatgg atatcttgag ccaagtttct gaaacttacc
    32821 gtaagattcg taacactctt cgtttcttga ttgccaatac atctgacttt aacccagctc
    32881 aagatacagt cgcttacgat gagcttcgtt cagttgataa gtacatgacg attcgcttta
    32941 accagcttgt caagaccatt cgtgatgcct atgcagactt tgaattcttg acgatctaca
    33001 aggccttggt gaactttatc aacgttgact tgtcagcctt ctaccttgat tttgccaaag
    33061 atgttgttta cattgaaggt gccaaatcac tggaacgccg tcaaatgcag actgtcttct
    33121 atgacattct tgtcaaaatc accaaactct tgacaccaat ccttcctcac actgcggaag
    33181 aaatctggtc atatcttgag tttgaaacag aagacttcgt ccaattgtca gaattaccag
    33241 aagttcaaac ttttgctaac caagaagaaa tcttggatac atgggcagcc ttcatggact
    33301 ttcgtggaca agcacaaaaa gccttggaag aagctcgtaa tgcaaaagtt atcggtaaat
    33361 cacttgaagc acacttgaca gtttatccaa atgaagttgt gaaaactcta ctcgaagcag
    33421 taaacagcaa tgtagcacaa cttttgatcg tgtctgagtt gaccatcgca gaaggaccag
    33481 ctccagaagc tgcccttagc ttcgaagatg tagccttcac aattgaacgt gctgctggtg
    33541 aagtatgtga ccgttgccgt cgtatcgacc caacaacagc agaacgcagc taccaggcag
    33601 ttatctgtga ccactgtgca agcatcgtag aagaaaactt tgcggaagca gtcgcagaag
    33661 gatttgaaga gaaataagat tgaaaagtct aggcaaaatt caatttgaga agaaaagaca
    33721 actaatttta tagtctatta aacgcattgt atcacgtttt tgaatacctg atatgatgcg
    33781 ttttttattt attttaaaaa tttgcgaggt atgacttttt atactcaaca agaatcaaag
    33841 agaaacttag caatctaaca gtagtaagat aaaataggaa tttgatatta gggataagat
    33901 tggtaa
//
LOCUS       DAVKJD010000014        31234 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKJD010000014 DAVKJD010000000
VERSION     DAVKJD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31234
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.014"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(24..437)
                     /gene="ndk"
                     /locus_tag="VW157_000724"
     CDS             complement(24..437)
                     /gene="ndk"
                     /locus_tag="VW157_000724"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9552060.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(548..4225)
                     /gene="rpoC"
                     /locus_tag="VW157_000725"
     CDS             complement(548..4225)
                     /gene="rpoC"
                     /locus_tag="VW157_000725"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9552061.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMSYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(4258..7869)
                     /gene="rpoB"
                     /locus_tag="VW157_000726"
     CDS             complement(4258..7869)
                     /gene="rpoB"
                     /locus_tag="VW157_000726"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9552062.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            8285..8355
                     /locus_tag="VW157_000727"
     tRNA            8285..8355
                     /locus_tag="VW157_000727"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8317..8319,aa:Cys,seq:gca)
     gene            complement(8553..9884)
                     /locus_tag="VW157_000728"
     CDS             complement(8553..9884)
                     /locus_tag="VW157_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9552063.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(10175..10999)
                     /gene="endA"
                     /locus_tag="VW157_000729"
     CDS             complement(10175..10999)
                     /gene="endA"
                     /locus_tag="VW157_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9552064.1"
                     /translation="MNKKTRQTLIGLLVLLLLSAGSYYIKQNELGNQAAKTKMSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(11038..11226)
                     /locus_tag="VW157_000730"
     CDS             complement(11038..11226)
                     /locus_tag="VW157_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9552065.1"
                     /translation="MNKKSGYVIKRLLLVILVLILGLLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(11219..12502)
                     /gene="murA"
                     /locus_tag="VW157_000731"
     CDS             complement(11219..12502)
                     /gene="murA"
                     /locus_tag="VW157_000731"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9552066.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(12526..12756)
                     /locus_tag="VW157_000732"
     CDS             complement(12526..12756)
                     /locus_tag="VW157_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9552067.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALGGLL"
     gene            complement(12814..13851)
                     /locus_tag="VW157_000733"
     CDS             complement(12814..13851)
                     /locus_tag="VW157_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9552068.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(13835..14323)
                     /gene="coaD"
                     /locus_tag="VW157_000734"
     CDS             complement(13835..14323)
                     /gene="coaD"
                     /locus_tag="VW157_000734"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9552069.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(14313..14852)
                     /gene="rsmD"
                     /locus_tag="VW157_000735"
     CDS             complement(14313..14852)
                     /gene="rsmD"
                     /locus_tag="VW157_000735"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9552070.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSNAVLVERGRKAQTIVAENIQMTKEIGKFQLLKMDAER
                     ALERVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(14917..15909)
                     /locus_tag="VW157_000736"
     CDS             complement(14917..15909)
                     /locus_tag="VW157_000736"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9552071.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(16021..16704)
                     /locus_tag="VW157_000737"
     CDS             complement(16021..16704)
                     /locus_tag="VW157_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9552072.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(16760..17500)
                     /locus_tag="VW157_000738"
     CDS             complement(16760..17500)
                     /locus_tag="VW157_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9552073.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVATGILIFHLS"
     gene            17534..17812
                     /locus_tag="VW157_000739"
     CDS             17534..17812
                     /locus_tag="VW157_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9552074.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            17890..18816
                     /gene="yidC"
                     /locus_tag="VW157_000740"
     CDS             17890..18816
                     /gene="yidC"
                     /locus_tag="VW157_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9552075.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(18891..19685)
                     /gene="pflA"
                     /locus_tag="VW157_000741"
     CDS             complement(18891..19685)
                     /gene="pflA"
                     /locus_tag="VW157_000741"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9552076.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(19811..21061)
                     /locus_tag="VW157_000742"
     CDS             complement(19811..21061)
                     /locus_tag="VW157_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9552077.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(21128..21955)
                     /gene="purR"
                     /locus_tag="VW157_000743"
     CDS             complement(21128..21955)
                     /gene="purR"
                     /locus_tag="VW157_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9552078.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(22222..23163)
                     /locus_tag="VW157_000744"
     CDS             complement(22222..23163)
                     /locus_tag="VW157_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9552079.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(23153..24409)
                     /locus_tag="VW157_000745"
     CDS             complement(23153..24409)
                     /locus_tag="VW157_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9552080.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYEVGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(24411..25073)
                     /locus_tag="VW157_000746"
     CDS             complement(24411..25073)
                     /locus_tag="VW157_000746"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9552081.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLLLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEKNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(25036..25692)
                     /locus_tag="VW157_000747"
     CDS             complement(25036..25692)
                     /locus_tag="VW157_000747"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9552082.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(25703..26581)
                     /gene="rsgA"
                     /locus_tag="VW157_000748"
     CDS             complement(25703..26581)
                     /gene="rsgA"
                     /locus_tag="VW157_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9552083.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(26583..27455)
                     /gene="rsmA"
                     /locus_tag="VW157_000749"
     CDS             complement(26583..27455)
                     /gene="rsmA"
                     /locus_tag="VW157_000749"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9552084.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(27841..27999)
                     /locus_tag="VW157_000750"
     CDS             complement(27841..27999)
                     /locus_tag="VW157_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552085.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEG"
     gene            complement(27992..28633)
                     /locus_tag="VW157_000751"
     CDS             complement(27992..28633)
                     /locus_tag="VW157_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552086.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNHLVWNKADEIIDMRKLAHV"
     gene            complement(28635..30665)
                     /locus_tag="VW157_000752"
                     /pseudo
     CDS             complement(28635..30665)
                     /locus_tag="VW157_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(30742..31038)
                     /locus_tag="VW157_000753"
     CDS             complement(30742..31038)
                     /locus_tag="VW157_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9552087.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
BASE COUNT         8760 a         7351 c         6101 g         9022 t
ORIGIN      
        1 caattgattg agccaatcca ctcttaaaac caaagagcaa tttctcgctt agctgactct
       61 tctgaatctg aaccatgtac aacattttgg ataatctcat tttctccagc agcttttgca
      121 aaatcacctc gaatagtgcc tggtaaagct tcttctggac gagttgcacc catcatggtc
      181 cgccaagttt cgattacttt gggaccagaa atgacaccca caagaactgg acctgaagtc
      241 atgaattcac gaatcggtgg gtaaaaactc tgaccaacca agtcctgata gtgctggtca
      301 atcaactctt ctgaaacctg tgaacgaaac tccaattttt cgattgtaaa tccacgttgt
      361 tcgatgcgct ttaacacttc acccactagc cctcttttta caccatctgg tttgatgata
      421 aagaatgttt gttccatacc cgtctccttt gtcagcttct ttcttttatt ttaccacatt
      481 tcgtggaaaa atggagaaag ttttcagaag agagaatgag agaacccgag ggttctctca
      541 ttctctctta ttctactgtt tcttccacag tttcaacggc agtatccaca actacttctg
      601 ttgtttcttc atttccttct tcctctactg gaggattaag gtattcttct tcgttgacag
      661 catgtggttc aaggttacgg taacgggcca taccagtacc agctgggatg atcttaccga
      721 tgataacatt ttctttaagt ccaaggagat ggtctttctt accacggata gctgcgtcag
      781 taaggacacg agttgtttcc tggaaggaag ccgctgacaa gaaactgttt gtttcaagtg
      841 aggctttggt aattcccata aggactgggc gacctgtcgc tggaactcca cctgcgataa
      901 ggacatcttt gttggcatct gtaaagtcat tgatatccat gagggtaccc atgagaagat
      961 ctgtatcacc tggatccatg acacggactt tacggatcat ttgacgaacc attacctcga
     1021 tgtgtttgtc accgatttct accccttggc tacggtaaac tttttgtact tcaccgagaa
     1081 ggtacgtttc aactgacaag acatcacgaa ctgcaaggag acgttttggt tggatagaac
     1141 cttctgtcag agcagcacca cgcgctactt ggcccccaac ttcgacacgc atacgagctg
     1201 taaatggaac gacatattca ccttcgccag tttcaccctt aacaaagact ttcttggtac
     1261 gagttgatgc atcctcttcg atagcagtaa cttgtccttt aacctctgtg ataaccgctt
     1321 cccctttagg attgcgggct tcaaagattt cttggacacg aggaagaccc tgagtgatat
     1381 cggtatttga ggcaacccca cctgtgtgga aggtacgcat tgtaagctgt gtaccaggtt
     1441 ccccgataga ttgggcagcg attgtaccaa ctgcttcacc aacttcaacc gcatcaccag
     1501 tcgccaagtt gataccgtaa cagtgacggc agacaccgtg acgagtgtta catgtaaata
     1561 cagaacggat agtcacttct tccacaccag cattgacaat ttcacgcgcc ttgtcttctg
     1621 taatcaattc atttggacca ataatcactg caccagtttc tggatgttta acagttttct
     1681 tagtgtaacg accgttgaga cgctcttcga gagactcgat catctctttt ccttctgcga
     1741 tagaacggat caagagacca cggtcagttc cacagtcgtc ctcacggatg ataacgtctt
     1801 gggcaacgtc gaccaaacga cgagtcaagt aacctgagtc ggctgtctta agggccgtat
     1861 cggtcatacc tttacgagca ccgtgagttg agaagaacat ttccaatacc gacaaacctt
     1921 cgcggaagtt tgaaaggatt ggcaattcca tgatacgtcc attcggagca gccatcagac
     1981 cacgcatacc ggcaagctgt gagaagtttg agatgttacc acgggctcca gagtccatca
     2041 tcataacgat tgggttctta ggatcttggt tagcaatcaa gcgtttctca agtttttcac
     2101 gggcagcacg ccattcagct gtaacagcat tgtaacgctc gtcgtctgtg atcataccac
     2161 gacggaattg tttggtgatt tgttcgacac gtttgtgtga ttcttcaatg atttcagcct
     2221 tgtcatcaac gactgggatg tcggcaatac ccactgtcaa tcctgcaaga gttgagtggt
     2281 ggtaaccgag gttcttcatg cggtcaagta gggcagaagt ttctgtcgta cggaaacgtt
     2341 tgaagatttc agcgatgata tttccgaggt ttttcttctt gaatggaggg ttgagctcaa
     2401 gattgctgat agcttccttg atatctccac caagtggcaa gaagtattta gctggaacac
     2461 cttctgtcaa gttggcattg tttggttctt gcaagtatgg tagcccctct ggcatgatat
     2521 cgttgaagag aattttacca actgttgtaa gcaagacctt atgtctttgc tcttctgtcc
     2581 aaggcttgtt gaggctgtct gttgcgatac caacacgtga gtggaggtga acataaccat
     2641 tgcggtaaga cataaccgct tcgtcacggt ctttgaagac cattccttca ccttcgcgac
     2701 cagcttcttc catggtcaag tagtagttac ccaaaaccat gtcctgagat ggagtaacta
     2761 ccggtttccc atctttcggg ttcaagatgt gctcagcagc tagcatgagg atacgagctt
     2821 ctgcttgtgc ttcttctgaa agtggtacgt ggatggccat ttggtccccg tcaaagtcag
     2881 cattgtaggc ttcacagaca agtgggtgca agcgaagagc cttaccatca attaagactg
     2941 gctcgaaggc ttggataccc aaacggtgaa gggtcggtgc gcggttcaaa agcactgggt
     3001 gttctttaat cacttcttca aggatatccc agatacgctc atctccgcgt tccaccaagc
     3061 gtttagctgc tttgacgttt tgcacgatat cacgggcaac gatttcacgc atgacaaatg
     3121 gtttaaagag ttcaatcgcc atttcacgtg gcacaccaca ttggtacatc ttaagagttg
     3181 gaccaacggc gataactgaa cgtcctgaga agtcaacacg tttaccgagc aagttttgac
     3241 ggaagcgtcc ttgtttacct ttaagcatgt ggctcaatga tttcaatgga cggctacctg
     3301 gtcctgtgat tggacgacca cgacgaccat tgtcaatcaa agcgtcaact gcttcttgaa
     3361 gcatacgctt ctcattttga acgatgatac ctggtgcatt taactcaagc aaacgagcca
     3421 aacggttgtt acggttgata acacggcggt aaaggtcatt caagtcagat gaggcaaaac
     3481 ggccaccatc caactgcaac attggacgaa gatctggtgg gataaccgga aggatgttaa
     3541 gaatcatcca ttcaggtttg tttccagact tgtaaaaggc atccaaaaca tccaaacgac
     3601 ggatggcttt gacacgtttt tgtccagtag ctgttttcaa ttcttctttg agttcagcaa
     3661 tttctttttc aagatctact tgcttcaaaa ggtcttggat ggcttccgca cccatcttgg
     3721 caacaaatga accataacca tattcacgca agcgctctcg gtattcgcgc tctgtcatga
     3781 tagacttgtg ctcaagtggt gtatccttag gatcaatcac cacataagcc gcaaagtaga
     3841 taacttcctc gagggcacga gggctcatat caagggtcaa gcccatacgg cttggaatcc
     3901 ccttgaagta ccagatgtga gatacaggag ctttcaattc gatatgtccc atacgctcac
     3961 gacgaacttt cgtacgcgtt acttcaaccc cacagcggtc acaaacaatt cctctgtaac
     4021 gaatgcgttt gtacttacca caagcacatt cccagtcttt tgtaggacca aagatcactt
     4081 catcaaagag tccttcacgt tctggtttca aggtacgata attgattgtt tcaggttttt
     4141 tgacttctcc ataagaccat gaacggactt tacttggaga agctagggtg atttgcatac
     4201 ttttaaaacg atttacatca accactattt cttccctttc tattctaagt gaactgctta
     4261 ttcttgttca gcagcttctt ctgttgcttc cgcttttgtt gctttctcag cttcttcagc
     4321 ttcaaaggct gctttagcct cttgggctgc tttttcgcgg gctttttcaa ggtcatctac
     4381 gtggatgaca tcttcgtcca ttccttcatc caagtcgcga agttccactt cttggtcatc
     4441 ttcgtctagg acacgcatgt caagaccaag agattgcaat tctttgacaa gaactcggaa
     4501 ggattctgga acacctggtt ttggaattgg tttgcctttt gtaatagctt cataggcttt
     4561 caaacgtccg ttgatatcgt ccgacttgta agtcaagatt tcttgaagga catttgacgc
     4621 accgtaggct tcaagagccc aaacctccat ctcaccgaaa cgttgtccac caaactgagc
     4681 tttacctccg agtggttgtt gggtaacagt tgagtaaggt ccgactgaac gcgcatgcaa
     4741 tttatcgtca accatgtggt ggagtttgat catgtacatg actccaacag aaacacggtt
     4801 atcaaatggt tcacctgtac gtccatcgta aaggatggtc ttggcatcgc tatccatacc
     4861 tgcttcttta acagttgacc aaagatcttc agaacttgct ccatcaaaga ctggtgtcgc
     4921 aatgtgaata ccaagagtac gagctgccat accaaggtga agctccataa cctgaccgat
     4981 attcatacgt gatggcaccc caagtgggtt caacatgatg tcgactggag ttccgtctgg
     5041 aaggtaaggc atgtcttcta caggaacgat acgagagaca acccctttgt ttccgtgacg
     5101 tccggccatt ttatctccga ccttaatctt acgtttttga gcgatgtaaa cacgaaccaa
     5161 catgttaaca cctgattgca actcatctcc atttacacgt gtaaagatct taacatcacg
     5221 aacgacacca tcggcaccgt gtggtacacg aagagaagta tcacgcactt cacgagactt
     5281 gtctccaaag atagcgtgca agagacgttc ttcagctgaa agatctttct cacccttagg
     5341 tgttacttta cctacaagaa tatcaccttc tttaacctca gcaccaatac ggataatccc
     5401 catttcgtca aggtctttga gggcatcttc accaacgttt ggaatttcac gagtgatttc
     5461 ttcaggccca agctttgtat cgcgcgtttc tgattcgtat tcttcaaggt gaacagatgt
     5521 gtagacatcg tccttcacca agcgttcgct catgataacg gcatcctcga agttgtaacc
     5581 ttcccaagtc atgtaggcaa cgattgggtt ttgtccaagc gccatttctc cattttccat
     5641 agaaggtccg tcagcgatga aatcgccttt ttcaacgaca tcaccaactt ttacgagagt
     5701 gcgttggttg taagcagtac ctgagtttga acgacggaat ttttggatgt ggtaaacatc
     5761 caatgaacca tcttcacgac gaacttctac cttgtcagca tctgcgtaag taactttacc
     5821 atcatactga gcaatcacag ccgcaccaga atcgtgggct gcttggtatt ccataccagt
     5881 accaacgtaa ggtgcctgag gattaatcaa tggcacagcc tgacgttgca tattggctcc
     5941 catgagggca cggttggagt catcgttttc taagaaagga atacatgctg tcgcaacggc
     6001 aactacctgt tttggtgaaa cgtccatgta gtcaacaata ttagctggat actcttggtt
     6061 gaccccttgg tgacgtccca tgacaatctt ctcagcaaag gttccatctt cattcagacg
     6121 agagttagcc tgagctacag tatattcatc ttcttcatca gctgtcaacc aaacaatttc
     6181 gttcgtgaca acacctgttt cacggtcaac cttacggtat ggtgtttgaa caaaaccata
     6241 tttgttcaag tgtccgtaag atgacaagtt attgatcaaa ccgatgttag gtccttcagg
     6301 tgtctcgatt ggacacatac gaccatagtg agtgtagtgc acatcacgta cttcatatcc
     6361 ggcacggtca cgagtcaaac caccaggtcc taaggctgac aaacggcgtt tgtgagacaa
     6421 ctcagaaagc gggttgtgtt ggtccatgaa ctgtgacaac tgtgatgaac caaagaattc
     6481 tttaactgca gctgttacag gacggatatt gataatttgt tgtggtgtca agacttcatt
     6541 gtcctgaaca gacatacgtt cacggacatt acgttccata cgagaaagtc ccaaacgtac
     6601 ttggttggca agcaattcac caaccgcacg gatacgacga tttccaaggt ggtcgatatc
     6661 atctacacgg ccaagtcctt cagccaagtt gaggaagtag ctcatctcag caaggatatc
     6721 tgcaggagtc accgtacgaa ccttgtcatc tgggttagca ttaccaatga tcgttacgac
     6781 gcgatctgga tcagttggag caacaacctt gaatttttga agaacaacag gctcagtcac
     6841 aacggctgca tcgtttggga tgtagacaat cttgttcaag tcgccatcca aatggctttc
     6901 aatgctttca atcacgctac gagtcataat cgtaccagct tctaccaaga tttctccagt
     6961 ttcagggtct accaatggct ctgcaatggt ttggttgagc aaacgtgttt taacattgag
     7021 ttttttattg attttgtaac gaccaactgc tgccaagtca tagcgacgtg ggtcaaagaa
     7081 gcgagccaca agcaagctac gtgagctttc agctgtctta ggctcacctg gacgaaggcg
     7141 ttcgtaaatt tctttcaagg cttcgtctgt acgagagtcc attggattct tgtggatatc
     7201 tttttcaaca gtgttgcgaa ccaattcgct gtcaccaaag atatcaaaga tttcatcatc
     7261 acctgagaaa ccaagagcac gaaccaaggt tgtaaatgga atcttacgag tacggtcgat
     7321 acgagtgtag gtgatatctt ttgagtcgct ttcaagttcc aaccaagctc cacggttagg
     7381 gataacagtt gaaccatagc ccaccttacc atttttgtct actttgtcgt taaagtaaac
     7441 acctggtgag cggaccaact gagaaacgat aatacgttca ccaccattga tgatgaaagt
     7501 acccatttct gtcatgattg ggaaatcacc aaagaaaact tcttgggtct tgatttcgcc
     7561 tgtttcttta ttgatcaagc ggaaggttac aaaaattggt gctgagtagc tagcatcgtg
     7621 aatacgagct tcttctagcg tgtattttgg ttccttgatt tcatatccaa caaattccaa
     7681 ctccattgtg tctgtgaagt ttgaaattgg caatacatct tcaaacactt ccttaagacc
     7741 gtggtctagg aaagctttga atgagtcagt ttgaatttca atcaaatttg gtaagtcaag
     7801 aacttctttg attcttgaaa aactacgacg ggtacgatgt ttcccgtatt gaacgtcatg
     7861 tcctgccaag atgattctcc tttgtaaata agttccaagc cttgtcaatc aggcttttct
     7921 aatcgccata tggttgtaaa ccccttatca ccgtgtcctc ttgacgaatt ttcagaatct
     7981 ttaagcctct gttacaaatg ctcaaaatct tgaaaaaaag cacaaaaaga gcagctaaat
     8041 ctgacttttt cagaagattt aactgctgtg agccttgtct ggacaatatt tcagacaaaa
     8101 cctacgacaa atgattaccc atattatacc ctatttagct agatttttca aggggtttca
     8161 gtaggtttgt ggtaaatttt ttcccataga aaacttggca tcacattcga atcacgctat
     8221 ggtacaaaaa actgaaaaaa ctattgactg aaaatcattt tcaaggtata ataataaacg
     8281 ttaaggcggt atagccaagt ggtaaggcac ggctctgcaa aagcttgatc gtcggttcaa
     8341 atccgtctac cgccttctat aacttgattt atcaggtttc aaatgaacag aaagcccaat
     8401 ttgaagggct tttcttattt tccctcgaat aaatacgtat aactttaaaa acttttggag
     8461 cgagtttgtg gcagagttct ttccatggca taattccctt ttgaaatcaa ttgaaaaaag
     8521 cttcgtcacg cctggtgaca aaagcctcta gtttattctg tttcttcttc tatttcgact
     8581 tctgtgattt ggacttttac cttggtaaca cgtccattct tcactttatc attggttaag
     8641 ataaactgtt tgttttggct gaccaattca tagctgagtt tctcagtcgt tggaatggtt
     8701 cccactcctg tcaaataata accagcgata gtatcaacgt catcactttc cagttcaaca
     8761 ttaaagtaat tattgaaatc attgagggtc atagttcctt gaacaatgta ggtatcctcg
     8821 ccaatctgat gaacatctat tgctgctttg tccgtttcat catcaatttc accaacaatt
     8881 tcctctaaga ggtcttccaa agtcaccaaa ccagccatac caccgtattc atccagcaag
     8941 attgccattt gtctttgggt atttcgcagt tcttttagca agtcatccac aaaaatagtt
     9001 tcaggtacaa aaagtggatc ttgtaaaatt ctcttccaaa caatattgtc aaaaccgtcc
     9061 acaaagcctg ccttaaggag actcttggtg tgaatgattc caattacatt gtccttatcc
     9121 ccatcataaa ccgggatacg agaataattt tgttttaaaa tactttggat aatggcttga
     9181 ctatcatcct gaatatccac cataaaggca tccgttcgag gaaccataac ttctcgtgcc
     9241 atcagttcat cgagcgaaaa gacaccttgt agcatctcaa tctcatcagc atccaatgtt
     9301 tcttcactat ttgtcagcat ataggcaatt tcatcacggg tcatcttttc atccgcatca
     9361 tcgaatgaca taggagtcaa atggctcaag aaattggtcg aagcagctaa aagccaaaca
     9421 aaaggactga ctagttttcc gatcccaatg ataaccggcg ctgtacgaat tgccaaggca
     9481 tcctttagat taagagcgat tctcttagga tataattccc caaaaacgat ggaaatatag
     9541 gtcaaaaatg ccaaggatag aaaagttgcc acggcttgtg ctgtttcgcc attcccaagc
     9601 caagaggcaa tcacacgtcc tagagtatca gttaaactcg cccctgataa gattgtaatc
     9661 agggtgattc ctacctgaat ggttgataaa aagtggttag gattttctag taccttcagc
     9721 aggcggatgt agcgtctgtc tccttcttcc gccttttgtt caactcgggc acgattaaga
     9781 gaaaccatgg ccatttctgt ggctgagaaa aaggcattta ataccgtcaa gataaacaat
     9841 aaaacaaatt gtagcaacaa attctgacta cttgggtctt ccatagttct tctcctaaaa
     9901 tagtatctta tcctttatta tatcacaaaa tttaatccaa tacatattat ttttttctta
     9961 gcgtaacttc catctctctc ccaaaactgg aagttagtct aagacggatt tccactgatt
    10021 tcacgcgttt ttctcatact aaaagctagg ttgtgggaaa ctggaagtta gttttagaag
    10081 ttactatttt ttcttagtac ctgttctagc cctgcttaaa ctagtaaaaa gaactcctac
    10141 atccataagt gacataggag tttaggctta tcttttactg agttacagtt acttctccag
    10201 ttcggtaatc cagttgaagt cccttttgaa cattgggaac tagaacattg aattccaatt
    10261 ctccatccga agacttggct tcaatctgtg aagctgaggg aactaaatcc tcgtttgaag
    10321 cgtagtaaag ggttacacgg taacggacac gcttgttttg gtccaaggct ttacgcacct
    10381 tgctttcata gtagttttga ccagtcgaat actcggcttg tgcctgattt gcccaggctg
    10441 tctgaacagc aatgttttta ggattgcttg ttgaggcatc aaaaccatcc aaaccaccga
    10501 ttaaggcata gcctaacaaa tgacctctat cgactgcatg ggtataagag ccctttagat
    10561 tcttgacctg atgccaacct ggaggagtcc aagaagttga accattccca gtttctttac
    10621 gattcttgta ctgacgagtg gccttagaca agagagcatt agctacggtt ggaacagttt
    10681 ccttgcccac tgtctttgtt ttattgtcag cgtagggctt acttgaaacc ttggcatcta
    10741 gatttgtttt attaccattg acgataaaag cacctgagcc attccactcc agactcccct
    10801 ttatttgact cttgactgcg tctgttaaga cactctctgc caatgcttga ctaggagctt
    10861 cagacgcttg ttttttctga ctcatttttg tcttagcagc ctgatttcct agttcattct
    10921 gcttaatata atagctccct gcagacaaaa gcaataacac tagcagtcca atcagtgtct
    10981 gtcttgtttt tttgttcata tttctcctta tctttataaa aaggctggtc tcccagccta
    11041 atttcctgta aatttatgaa tcaattcctg ccattttgcc ggagacagga tagcccacgg
    11101 atcttgaccc ttgcccaaga ttccataacc taccattaga ccgattccta aggctagaag
    11161 gcctaaaatc agtaccagaa tgactaaaag taaacgctta attacgtaac ctgatttctt
    11221 attcatcttc atcacttgcc tcaatccgct gaatcttagc acctagctgc gccaacttct
    11281 catggaaacc gtagtaacct ctatccaagt gaaccaattt accgaccaca gtttctccct
    11341 gtgctaccaa acctgtcaaa atcaaggccg cactggcacg aaggtcagtt gaaagaactt
    11401 ctgctccctg caaaggctgt ccaccaacaa tacgagctgt atcacggata atctcagaat
    11461 gcaagcccat gcggcgcatc tcttctaggt gttggaaacg attttcaaaa actgtctcca
    11521 ccatggttga ttcgcctttt gcaactgtca tcaaggctgt aaattgagcc tgcatatctg
    11581 ttggaaatcc tgggtggggc aaggttttca catgaacagc ttttagattt tctagttgag
    11641 aacgaacacg aattccttcg tcttcttcaa ttacttcaac acccatttca agtaacttgg
    11701 caatcaaggg acggttgtgc tcccagacag cgtctcgaat caagacatca ccaccagtca
    11761 tggcagcagc taccataaag gttcctgctt cgatacggtc ttggactaca ttgtgagtcg
    11821 taccatgaag tttctcaaca ccagtaatgg ttatagtctc tgtaccagca cctttgacct
    11881 tggctcccat ttcattaagg agaatggcta agtcaacaat ctcaggttca cgcgcagcat
    11941 tctcaatcac tgtcacccca tcagccagag tcgctgccat catcaagttc tgcgttgcac
    12001 caacacttgg aaagtccata tagatatgag cgccatgcaa gcgttctgcc ttggcttcga
    12061 tgtaaccagc tgtctgacta atcttaaccc ccatagcttc cagacctttc aaatgaagat
    12121 caataggacg gctaccaatc gtacaaccac ctggcatgga taccttggca tgacccacac
    12181 gggcaaggat tggccctaat acaacgatgg aggcgcgcat cttgctgaca tacttgtaag
    12241 gggcttcctc agtgatgtcg ccagtagcat ccaccttgac aagatgagct tcctcatcaa
    12301 agtcaacctt ggcattcaaa ccaccaacta cctgattcat aataaagaca tccgacaaaa
    12361 tcggaacatt ctgcaagacg gtctttcctt cacttgctag aatagtcgct gccaacaagg
    12421 gtaagactgc attttttgct ccctcgatcg tcacgcttcc taccagacga ttatcgccac
    12481 cttgaaccac aattttatcc atactcgttt cctttactct tgattttata ataatcctcc
    12541 aagcgcttct tgccacaaat gatacaggct aataaagaaa gaactcagga tgtagcctat
    12601 gacaatgcta aaaaaggcta ctaataaacg aacttttctt gtattatcat ctgtcacttt
    12661 taaaaccttc tcccatctaa caagatcctt taaaaggtaa aaactcacat aaataaagaa
    12721 catatgacta cttagagtga ataataattg aaccatttga ctattatacc atcttctctg
    12781 tttgtgcgct agtttggaaa cttcactcaa aaattaggga ttgtttttca agtaatcaat
    12841 cgcatcttgt agggttttaa cgggcacgat tttcatatcc gtcttgattg ttttagccgc
    12901 ttctagggct gtttggtagt tgtttttcgc gtccggatgc gccttttgtt cttcttcgct
    12961 aacagggtta tcaggagcaa agaaaatagc agcacctgcc ctagccgaag ctacaacctt
    13021 cttatcaata cctccaatgt ctcccacatt accatcgcgg tcaatggtac ctgtaccggc
    13081 aacaatacga ccattacgaa gatctgggtg agctatttga gtatagatag ctagactaaa
    13141 catgagacca gcacttggac cgccaatacc agctgttgaa aagctaattg ggacattgct
    13201 gattacctct gtacggtcaa tcaagccgat tccaattcca tttttgccat tttccaaggt
    13261 gatgattttt ccttctgcag acttggtttg cccatcctct tcataggtga ccttgacgga
    13321 atcccctaat ttttgagaac tgacgtaatc aatcaagtct ttggaactat caaaggtctg
    13381 atcattgact gctgtgaccg tatcagagat attgagaatc cctttaaagg ttgaattatc
    13441 cgtcacattc aaaacataaa ctccaaagta cttgagttcg atatccttac cagctgtttt
    13501 tagtccttga tacttggcca tattttgcga tgtttgcatg tagaattgat tgattcgcat
    13561 aaattcaaca tcggaagaac cacctgtagt ctcctgagca ctacgaatat ctgtaaaagg
    13621 tgtcaaccaa gcataaatca tatgagctaa agtggcatgt tgaacaccaa ccgtaacgaa
    13681 ttgataggca ccagcttcct tatcttctgt gtcatttact ttaaggactt ggcgaatatc
    13741 ttccgaacca cctggaacct ctatataata aggcaagggc actacaaatg ccaagaaagt
    13801 cacaatcaag gccgcaatga cgtataaggg ccatctaatc ttttttttca tttcttattt
    13861 cctccaaaat actctcggga acatagcagg caatatcctg accaaacttc aaaagctctc
    13921 taacgcctga tgaactgata tagagatgtt caggtcgact atgtaaataa atagtctcta
    13981 tatcagaaga cagctgatga ttgtagtaat caaaactggc ttcatattgc aaatccgacg
    14041 cattcctcaa accacgcact agacaagtag cacccaatct ttttgcaaca tcgaccacca
    14101 attcatcatg agaagccacg acttcaacat tttccaaatg tcccaaagcc ttttctagcc
    14161 cccgtttacg attttcaaga gggagaaatc cttgtttgtg gggattaaaa aaaataccca
    14221 cacaaagctt atcaaaaagt ctgctcgccc gttcaatgat atccagatgc ccatttgtca
    14281 tcggatcaaa tgagcctgtg aataagccaa tcttatctga catagactgt caccttacta
    14341 attccataaa tcttttcctt ccagataccc agacaggcaa tttcttctgg aagttcaacg
    14401 gccttatccg tctcgcacac aaccataaca tcttcagaaa aaagctctct ctcagccatt
    14461 ttttcaatat ctgctacgat ttgttccttg gcatagggag ggtctaagaa aacgaggtca
    14521 aattctccag atacccgttc caatgccctt tctgcatcca tcttgaggag ttgaaatttt
    14581 ccaatttcct tggtcatctg gatattttca gccacgatgg tctgagcctt acggcctcgc
    14641 tccaccaaaa cagcattgga catgccacgc gatactgctt cgatagataa accaccacta
    14701 cctgcataaa ggtccaagac tcgtccccct tcaaagtagg gaccaatcat gttaaaaatg
    14761 gctcccctaa ccttatccga agtaggtctt gttgtcttgc cttctagtgt cttgagggga
    14821 cgtcccccat agattcctga tacgattttc atactgttta ttataccaaa ttatagacaa
    14881 aaagagaaag aaaaccgaac cttgcggttc gattctctac aaaatatttt cgtaagtatc
    14941 gcggacttct tgaggccaaa cacttgtttg cacttctccg atgtgtctct tgcgaagtag
    15001 gaacatggcc atacgagatt gtccaattcc tccaccgatt gtcaatggga ataggccatt
    15061 caacaaagac ttgtgccatt ccaattctaa gcggtcttca tcacctgtaa tttccacctg
    15121 acgtctaaga gtttcttcat ctacacgaat tcccatagaa gacaactcaa aggctccacc
    15181 taaagactca ttccagacaa gaatatcacc atttagaccc ttgtagccat tctcagactc
    15241 gcttgtccag tcatcatagt ctggtgcacg tccatcgtgc ggtttaccat ctggcaactc
    15301 gccaccgata ccaatcaaaa agacggctcc aaattcttta caaatcgcat tttcacgttc
    15361 tttaggtgtc aagtctgggt agcgttctac caattcttct gtatggataa aggtgatttg
    15421 ttttggcaag atagactcga tgtcatagcg ggcttcaaca gctagctcag tcaggcgaat
    15481 agccttataa atcttctcaa ctgtttcttt tagataaacg atgttacgct taccatttgg
    15541 aataaccttc tcccagtccc actggtcaac ataaacagag tgggttgcat ccaaggaatc
    15601 ctcatctgga cgaagggctt tcatgtggac aaagagaccc tctccttcac caaagccaaa
    15661 acgagccaag gtgtggcgtt tccatttagc aagtgagtgc accacttcat aagtagcatc
    15721 agggatttgg agaaccttga ccgatactgg attttccaca ccagacaggt tgtcctgcat
    15781 tccgtcaccg accttactca agataggacc ttgaacttcg acaacttcta gcttatcttt
    15841 caaatactgg gtaaaagtgt ttttgacaaa ggaaatttct tcttgttgat ggataaaact
    15901 tttcttcata gacaactcct caaaaattta ataaacgtat tatacaccta tttccaagaa
    15961 aagaaaagag aaaatttaaa aaaaatcagt gtcactcaaa acactgattt catagacctt
    16021 ttagtcattg cgaccaaaga tacgtagaat acttaggaaa agattgataa aatcaagata
    16081 gatgctgaga gccattgaca ccacccagcc tgtcgctaca cgaccttgtg attgttcata
    16141 ggcgaggcga atcttttggt tatcccaagc aatcaaccct gaaaaaacca aaaccatggc
    16201 tacgctaatc atataatcaa agaagccact agccaagaaa atattgacta ccatagcaat
    16261 cagtaaacca atgagagctg ccatcattgc tcgaccaatc ccactcaagt ctttcttggt
    16321 aaaaatacca actgccgcca tgacaaagaa gagaagggcg ctcgatacaa aggcagataa
    16381 aactgtaccc ggagtataga aagccaccac aaaactaagg gtaaagccgt ttaaaacgga
    16441 gtaaagtaaa aatactggaa gagcagctgg actatttctt gaagccatgc tactggcaac
    16501 gaaaaccaaa gccaactccg caaaagtagc aatggtcaac cagagacgcc cctgcatcaa
    16561 caagtaaacc aactgagact gaaagaccgt caacataagg cctgatacca aagcagatag
    16621 tccgattccc agaccaacaa aggcataaac cttagcgtaa aattgattga gacctgcacg
    16681 gtcatgtata atagtgtgat tcatcctttt ctccttttct gtgaacatct ttccttgatt
    16741 ataacaaaaa aagttaaaat tagcttaaat ggaagattaa aataccggtt gcaactgcaa
    16801 cattcaaact ctcggcctgc cccttcatgc taatatggac caactggtct gcactttcag
    16861 ccataagggg actaattcct tgaccctcat ttcccataac tagtacaaaa ttttctatag
    16921 aaggcagttc tctgtaatca acagaatctt tagatagggt tgttgctagc actgggatag
    16981 ctaccttttt agtttcgtca agaagcgctt gactagtcat ccggtaaatg ggcagatgga
    17041 aatgactacc ttgcatggaa cgtagggttt tgagactgta gatgtctgcc gacttatctg
    17101 aaacaatcac tcctgtaaaa cctgctgcat ccgcagttcg aatgatagtt cctacattac
    17161 caggatcttg cacatcttcc aaaaacaaga acttgccctg actcaaatca gcttgtccta
    17221 cttcttcttt ttgaaccacg gcaacaattc cctgtggagt ctgagaatcc gccaaatcta
    17281 gcaaaatatc ctctgaaatc cagacagttt gcggaaaagc agctaactga tttcgataat
    17341 tttctagggc aaagattttc tcaatcgtca ctccagcttg gacagcttct tcaaacaagt
    17401 gccagccttc aatcaaatag gcagacttgc ggtatttttt ttggtgtaat ttcttggcat
    17461 tttttaccac agaattggct tttgaggtta taatagtcat agaagtatta taacacaatc
    17521 aaaggggttt ggtatgcaaa aggttagaat gattgcccaa ggtagggtgc agggagtcgg
    17581 ctttcgttgg ggtgtttata gcctggccct tgaaatcggt ggcatcacag gtcgggtatg
    17641 gaataacgac gatggcacag tggaaatctt agcccaagca gactcatctg ctatcatggc
    17701 aaaatttatc caagaaatcc gaaaaggacc gacacctttt tcaaaagtaa gctacttaga
    17761 tgtcaaacta agcaactttc ctccctactc tgactttaaa atcgcaaatt aggtctccag
    17821 aactattgta tattttgtaa aaaaacagta gaatagaaag gtataatttt taaagaagga
    17881 ataaaaacag tgaaatctat taaacgtttt gcactctcag ctatgggagc ggctatgttg
    17941 cttgtcttga ctggttgtgt caatgtcgat aaaaccacag gtcagccaac aggatttatt
    18001 tggaatacca tcggagcccc tatggctgaa gctatcaagt acttcgctac tgataaaggt
    18061 ctaggctttg gtgtcgctat catcatcgta accattatcg tgcgcttgat tatcttgcca
    18121 cttggtatct accaatcatg gaaggcaacg cttcactctg aaaagatgaa cgccctcaag
    18181 cacgtccttg agccacacca aacgcgtctc aaagaagcga ctactcaaga agaaaaactc
    18241 gaagcccaac aagctctctt tgctgctcaa aaagagcacg gtatcagcat gtttggcggt
    18301 gtaggatgtt tccctatcct ccttcaaatg cctttcttct ctgctatcta ctttgctgcc
    18361 caacatactg aaggggttgc tcaagcaagc tacctaggca ttcctctagg ttctccaagt
    18421 atgattttgg ttgcctgtgc tggtgtcctt tactatcttc aatcgctcct ttcacttcac
    18481 ggggtagaag atgaaatgca aagagaacaa atcaagaaaa tgatttacat gagcccactc
    18541 atgatcgtcg tcttctccct cttctcacca gctagtgtca cactttactg ggttgtcggt
    18601 ggtttcatga tgattctcca acagtttatc gtcaactata tcgttcgtcc aaaacttcgc
    18661 aaaaaagtcc gtgaagaact ggcaaagaac ccaccaaaag caagtgcttt ctcaacacca
    18721 agtggacgaa aagacgttac ccctgaacaa ccaactgcta ttacaagcaa gaaaaaacac
    18781 aaaaatcgca acgctggaaa acaacgttcg agataagaag aaaaaggctt agctgatttg
    18841 ctaagtcttt ttgcaagtca aaagcccgat gtttccatca ggcttctttt ctatccatgt
    18901 acacgtttca tataatcttg ataactttcg gtatccatga gttttttagc gttcttgacg
    18961 cgatctgctg ttggtggttt gactccttcg agggaatatg gaattccaag ttcacgccac
    19021 ttgaactcac ccatggtgtg ataaggtaga atttcaaact tatcaacatt tttgagggtc
    19081 ttgacgaact taccaagttc aatcaagtca tcatctctgt ctgtcaatcc tggaactagc
    19141 acgtggcgaa tccagacagg ttttccaata tctgatagat actgggcaca agccaagata
    19201 tttttattgg tttggctagt gacaatcttg tgctgttctt cgttgatttc cttgatatcc
    19261 aaaagaacca agtcagtgac agccatgagt ttgtcaaact tctcaaggta acgcggttta
    19321 ttacggaaag gaagagcaca ggtgtccaag gtacagtgga ttccttgttc cttagccttg
    19381 gtgaagagag caatcaggaa atcaatctgc aagagagctt ctcctccact gactgtaatc
    19441 ccacccttat ttccccagaa accacggtag cgcaaggcct ctgtcaagac atcatctacc
    19501 gtccgttcac gtgacttatt ggactccata gcccaagtgt ctgggttgtg gcaatactgg
    19561 caacgcatgt gacagccctg caaaaagaca ataaagcgaa taccaggccc atctactgac
    19621 ccaaagcttt ctgtcgaatg caccattcct gtcacttgtc cataatcaat tgtttcttca
    19681 gacatatcgt taccttcctt gaaaacgttt tatagtttta ttatatcacg acttgaagga
    19741 aaaacaagat ttttgcctat tttttagaaa gcaatctgtt tttcaatttc attttcaatc
    19801 tattatcaga ttattcttca aaatcgaagc catataaggt tgcaaaatag tcctcagggc
    19861 gctctgcacg gcggatttga cgggctttac cttcttcggt atagaggatt tccgcagaac
    19921 gtaatttggc attatactgg tagcccattg aaaatccgtg ggcacctgta tcatgaatta
    19981 ccagcaaatc accgatttct gtatgaggca gttcgcgatt gactgcaaat ttatcattgt
    20041 tttcacagag tgaaccaacc acatctaccg cttcagctgg tccatctgga tgggtcacgt
    20101 tgctaatatg atggtaagct ccgtacatag ctggacgcat gaggttgact gctgaggcat
    20161 ccacacctag ataggtacgg taggtttcct tcttatgagt cactcttgtg actagagcac
    20221 cgtgaggtgc cagcataaaa cgacccaatt cagtgaaaat cttgacctga ccaagacctg
    20281 ctgacgtaag aacttcttca tacaccttac gaactccctc accaatcaag gcgatatcgt
    20341 tcggctcctg gtctggacga taattaacac caataccgcc agaaagattg ataaagtcta
    20401 gccaaatgcc caacttttcc ttgatttcaa cagccagttc aaagagctga cgagccaact
    20461 ctggataata gagatgggtc acggtattgg acgctaggaa ggagtgaatc ccaaaagtct
    20521 tggctccttt ttccttcaag atagcaaagg cttcaaagag ctggtccttg gtcatgccaa
    20581 acttagcctc cccaggattg tccataatgt ctgtccccag ttcaaaaacg cctccaggat
    20641 tataacgaca agagatgatt tctggaatgc ctgctactct ctccagatgt tcaatatctt
    20701 caaaggcatc caagttaatg gtcgcaccca attcacgtgc ataggcgtat tccttgtctg
    20761 gcgtgttgtt ggaagagaac ataatctcag aacccagaaa gtccagttta tggctcatca
    20821 aaagctctac ataactagag cagtccacac cacaaccttc ttcttggaga attttcaaaa
    20881 tagctggagt tggagtagcc ttaactgcaa aatattcctt aaagcccttg ttccacgaaa
    20941 aagcttggtt gacggctctt gccttctcac gaatcccctt ctcatcatac aagtgaaagg
    21001 gagtcgggaa ctcggcaaca atcgcttcta actcttctct attgataaat ggtgttttca
    21061 taagcttcct tctattctgt ttgcaaagaa aggctgggac aatcgttcca acctttagtt
    21121 ctatctctta tttatcttcg tcaaagatat tgccaacctc aacatcgatg gcttggttct
    21181 tgacatcaat attggttacc ttcaagagtg acttgtagtc aaactgcttt tcacgttctt
    21241 cttgggcatt gtccgcaaag accgctacac ctgccagttc tgagtcgaac tcgcgcaaga
    21301 gactaatcat accattgacc gttccgccac ctttcaagaa gtcatccaca atcaagacac
    21361 ggctgcctgc cttaagacta cgttttgaaa ggaacatttt ctcgatacgg tcaccacttg
    21421 aacctgaaac atagttgacg ctaacagttg aaccttcggt aattttcagg tcacggcgca
    21481 caatgacaaa agagacattg aggacattgg caactgcatt tgcaagtggc acacccttag
    21541 ttgctacggt cataaccgcg tcaatttttt ggtccataaa gcttttggca ataatacgac
    21601 caatattttt caagatggct ggtgtgctaa gcaaatcaga cagatagata taaccacctg
    21661 gcaagatacg gtcactttct gacaacttgg tacgcaagtc ttcaaccatt tccttagcat
    21721 cctgactcga aatagacggt gtgaagatga cacctccgcc agccccagtc actgtctgga
    21781 tatgaccgat ttcaatttcc tcaaaggcgc gtttgataat gacgatatct tctgagatgg
    21841 atgatttagc agactcatac ttttcagcaa aagtattgag actagttagt ttataaggat
    21901 tattaatcaa atagttggaa atgacaacca tccgatcact tcttcttaat ttcatttcta
    21961 tttcctattc catttttatt caaaaaatca aaaagcaaac tagaaagctg gtcgctggtg
    22021 gttcaaaaca ctgttttgag attgtcgata gaactgacaa cccctgtaat atacctgcat
    22081 atatacatac gacaaggcga tactacccta gtttgaagag attttcgaag agtattcatt
    22141 tttgtctttt acttattata ccatattcac ataaaaaaac gaacattctt atcctaaaaa
    22201 atgctcattt ttcttaaatt attaatctaa atctggttta tagaaggaac gattatccat
    22261 agcgaagatt ttattggaca tctctccttt atccaccaaa gccagagctg ttgacatcat
    22321 catcatgctt gcatccagat tgtcaatcat atggataatc tctgcctcca taatacgtgg
    22381 acggactgga cttccatatt caagcaagcc gtggtgactg aggatgacat gacgaagcaa
    22441 aacgacttct tccttggtat catcgatgcc gagttccata actgtcttgg taatttcgct
    22501 atcaatcaga gcgatatgtc caagaagatt gcctctgacc gtgtactcgg tctggtctgg
    22561 tcccgtcaac tcgatgacct tagccaagtc gtgcaacata atccccgcat agagcagact
    22621 cttattgagc tgaggataaa cttcgctaat agcgtctgcc aaacgtacca tggtcgccgt
    22681 atgataagcc aaccccgttt caaaggcatg gtggttggtc ttggcagctg gataggagta
    22741 gaattcctta tcatacttgg tgtagagatt tcggacaatc cgttgccaga caggattttc
    22801 aattttgaaa atcatttgcg acatgtagtc acgaatttcc ttgacatcaa ctggtgactt
    22861 gaccttgaaa tcggctgggt cattgggttc accagcttga ggcaggcgga gagtaatttg
    22921 attgacttga ggggtattgt tataaacttc tcggcgtcct ttcatgtgga caaccttacc
    22981 tgcggtaaag gcctcaatgt tatgaggttg ggcatcccag agcttcccat caatctcgcc
    23041 actatcatct tggaaggtaa aggctaggta gtttttccca gctcgagttt gcctcaggtc
    23101 agctgatttg attaggtaaa agccttcaaa tagctcatct tttttcatgt gactaatctt
    23161 catattcttc ctcattttct tgaaaatgga gtagatcaag cgcaggctcg ccttctgaca
    23221 actcaatgtg acggagcgtc cgctcgatag ctatggtacg acggtttaat aattcatcaa
    23281 tattgccaga ggcatgttgg agatgttttt gtgccttgac cagaatacca ccaaacttgc
    23341 caaactcggt cttgacactg gcaagagtct tgctgatatg gtcggcactc ttttggatat
    23401 taagggtctt gaaaccaact gatagggagt taagaagggc tgatagggta cttggtcctg
    23461 caacgataat ctgttcttcc cgtctcaaat catcaaagaa gaccggattg cggacgattt
    23521 ctgagtagag accttctgtc ggaacaaaca aaactccaaa attggtcgtc cgaggtggtg
    23581 ctatgtactt gttcctaata tccctagcaa aacgcttgac acttgctagg agtgacttac
    23641 gacagcgttc gatttcatcc ttgtcaccta cttcataggc ttcttctaag cggtaataat
    23701 ctgccagtgg aaacttagag tcaattggta ggtagacgta ttcttggtcg ccttgtccgg
    23761 gtaacttgat ggcatactcc actcgttcac tagagttttc aaccgttgcg tattctcgtt
    23821 cgtactgggc aggtgtcatg atgtcttcaa taatttgccc cagttgcaat tctcccagaa
    23881 tccctcgcgt cttggttcca gagagaacct tgttaagagc tccgacatca cgggcaactg
    23941 tctgcatttc tccaagacca cgattgacag actccagttg tttagaaact gtctcaaagg
    24001 aagcctgtaa gcgtgtctgc aaggtctttt ctagtttttc ctcgaccgtc tggcgcattt
    24061 gttccaaacg ttgctcattt gattcctgca aggcttggag acgttggtct gtcttgtctc
    24121 tagtttggag gagattatct gtcatttctt gacggacttg ggtcaggcct tggtgcaatt
    24181 caatccgcac ttcttgcaaa cggtcgctga cagccacttc caaatctttt tggtctaact
    24241 ggctggcttg tctggcttgg tcaaagcggt aatccaactg gtctgacaaa tgatctgcct
    24301 gatcctccaa gctcttactt aagtgtttct cctgcctatc ctgcctttgc caaatcagaa
    24361 aaagaccagc taggttggca attaataata gaataagtaa actttccatc ctacctcctg
    24421 tccttgctat gcagtacgac cacataacca tctgggcaag ttaccgacac ttccctatct
    24481 atatattcgt tagaagcgta cactttttta aagaaaaaat tttcctctgt caactcatac
    24541 ttggctccaa gaatagtcag ctggctatcc cgaactggca taaaggctag atagtcgtag
    24601 tctgaacgag gttctagctg actggtccct tctggacaat aagtaatcaa gttttgccca
    24661 tcctcaattt ctatttgatg catatagggt gccaacttag gattgctagg cagaaagaca
    24721 ttggccaaca tatggtcaat acggccaccc aaggcaccga aaatagtaac ctgagcctga
    24781 ggatttttct caaagatggt taagagagcc aattccaaat cggtatcatc tttttctggt
    24841 cgggcttgga caaaatgctg ggcacgtttt tgaatcacct gtcgctcttc ttcggtcaca
    24901 gaatcaaaat ccccaactgc tagagcaaga agcaagtctt cttccaagac ccagagcgag
    24961 cctcgatcca ccccaacaaa agcatcaaaa tctgtccgat aatgaccgcg gtctccgcct
    25021 gcaaaaacgg caaccctagt ccagttgttt tctgagagtt tgtactcgct cattgacatc
    25081 tcccttaaag acataggaac ctgctacaaa aacggtcgca ccagcttctt tagcttgggc
    25141 aatagtttgg tcatcaatcc caccatccac ttcgatttca aagttcaaac ctttttcctc
    25201 acgaagagca accaactcac gaaccttatc catggtttct ggcagaaagg cttgcccacc
    25261 aaaacctgga ttaaccgtca tgactaaaac ttggtcaact agatgaagga cgtgcttgat
    25321 ggcttcaact gatgtgccag gattgataac gactgaaggc ttaactccga gtgaacgaat
    25381 tttttggagg gcgccatgaa tatgaggcgt tgcttctaca tggatactga tgatgtctgc
    25441 acctgcacgc gcaaaatctt ccagatgatg ctcagggttt gacaccatca agtggcaatc
    25501 gaaaaccatc ttactatgag gacgaaggct ctcgaccaca cctgcaccaa aactgatttg
    25561 cggtacaaaa tgactgtcca tgatatcgat atgggcatat tctgccccag ttgcttctag
    25621 acgtttgatt tcacgttcaa agttggcata atctgctgcc agaattgacg gagcaatctt
    25681 gtattgagac ataggtttct ccttattttg gaattttttt gctgactttt ttataggttt
    25741 ctctacgatt ttcaatttca ctaaggaatt gcaggtaatt gtcaaaacgg aaggttgcaa
    25801 taacaccctc ttcaacagct ggtttgacgg cacaagacgg ctcatgggta tgggtacaag
    25861 tacggaactt acaatctcgg ctaacagtag caatctctgg gaaagcctga ttgaggtctt
    25921 cagcccttga tacttcatag tccaaggatg aaaatcctgg tgtatctgcg attttacccc
    25981 cgttgagatt gtaaaaacta acagctcgag tggtatggcg accgcgacct agactgtctg
    26041 aaatttctcc cgtttcaaga ttgaggtctg gtgcgatttt attgagaaga gttgacttcc
    26101 caacacctgt ctgccccata aagaccgtaa ccttgcctgt taacaaagac aggagttcct
    26161 ctttactggt cacaaagtca tagccgatgt caccataggt ctgctggtaa aaatccagtt
    26221 ctcccctatc ttccaacaaa tccattttgg aaatatagac aatgggatgg atgcccttgt
    26281 gctccaaaag aaccaagaaa cgatccagca aattgctgtt aaaatcaggt tccttgacgg
    26341 acatgattac tacagcttga tcgatattga caataggcgg acgaaccaga ctgtttttcc
    26401 gttcgtgaat tttgaggata tagccttctg aattttcctc ggcagagaaa tctacccagt
    26461 ccccaacata aggggtatgg ccttttttac ggaaattccc acgcgcgcgt gtttgataaa
    26521 cctggccatc actctccaca tagtagaaac ctgccaaggc tttaatgatt tgtccctgca
    26581 tcttagagtc cttgcccttt aagtgcgtct gctagaccgg caaattctgc caagctgaga
    26641 gcttccccac gcacacttgg tgacaagcct gcctggtcca aagccttggt cagcttgtcc
    26701 ttgacctctt cagtcttacc aaagtaacct gtcaagttat tccacaaggt cttgcggcga
    26761 tgggtaaaac tagccttgga aaccttaaag aaaaagttct catcttctac tgctacggct
    26821 ggctctggac gacgcaccat tttcaagatg gctgaatcca catttggcgc tggcacaaag
    26881 accgtacgag gcacgataaa ggcaaccttg gctgtcatgt aatactgcac ggcgatagac
    26941 aagctaccgt aagccttggt gttaggctgg gctgaaatgc ggtccgctac ttctttctgc
    27001 atcatgacca caaactcact aaaaggaatg ccactctcaa tcaagtgcat gagaataggc
    27061 gtcgtgatgt agtaaggcaa attagccact accttgattg gcaggttagg atttttaaaa
    27121 ttttggatat gttgcgccaa atcaaccttg agaatatctt cgttaactac ggtcacatta
    27181 tcaaaatcac gcagggtatc tgccaaaatt ggcaccaaac ggtggtcaat ctcaaaagcc
    27241 atgacttggg ctgcacgctc agccaaaaat tctgtcaagg caccaatacc tggcccgatt
    27301 tcgatgacat tgacctgatc atcaatttca gccgtatcca caattttttg aaggatattg
    27361 gtatccgtca ggaaattttg cccgaaggac tttttaaagg taaaaccgtg acgctccagc
    27421 actgccttgg tcacgctata atctgcaatt ctcatctatt ctcttttcta ttatctgtta
    27481 ctactattgt aacacattcc ccttgtattt tgggaatgaa aatcatacca aaaaactatc
    27541 tttcctcatg aagaaagata gtcttggaaa tctttaaaga gggacttagc gcttctctcg
    27601 aaacatacgt tcatactctt cgaaaatctc ttcaaaccac gtcagcttca ccttgccgta
    27661 ctcaagtaca gcctgcggct agcttcctag tttgctcttt gattttcatt gagtatcaaa
    27721 aaaatcagct tcttgataac ttcgattttc gggtagggtc aaatccacat cctcctccac
    27781 atcatccact gtcagttcaa ccgtttgagg taaatttttc aaacgataat gcttttgcct
    27841 ttacccttca atactagcaa aatcaagcaa atagccatgc actcggattt gaaccaaaag
    27901 gagatgattg gtcatatttt ccatgatgag gatatcttct aaaccacctc tggcactagg
    27961 ataatcagat accctgcgaa tacggatttt ttcacacatg agcaagtttc ctcatatcaa
    28021 tgatttcatc agccttattc cagactaggt gattatgggt cgcaatgatg ataattcgat
    28081 tttcatcttt caaatccacc aagagattca taacctcctc tgaattttca ggatcaagag
    28141 ctgctgttgg ttcatctgcc aaaatcaagg gtggattttt caaaatagtc ttagcaaggg
    28201 caactcgttg ggcctctccc ccagataaag tatagatttt ttgttctaaa tccaaatacc
    28261 ctagattaac tttttctaaa gcccccactt gcctttccaa acgttctact tttgagattt
    28321 tctgaccaac aaaacccaaa tccaaatttt ctttgattga ttggttttct aagaggccaa
    28381 aattttgaaa gagatagccc atctgatctc gaaaatactc acgagtggga atggttttta
    28441 aatctttccc ctgatagaga acccttcctc catctatctt ttctagcttt cccaagatat
    28501 tcagcagcgt cgtctttccg cttccactct ttccgattaa ggcataaacc ttgcccttct
    28561 ccagcttgag attcaaacca gagaaaatcg cgcggtcatt aaatttcttt tccaatcctt
    28621 gaatatcaat catcttattt tcctttcaat actgccatgc taccagcttc ttcttttttg
    28681 tcctgcctta ctaaaatcaa gagggcgtta agcgtaaaca aagctacaac tagagcgcta
    28741 atcaaaccat ctcttgtcaa aatactagat aggactaggc caagcaagag aactcctcct
    28801 tgcgccagta aatacttgcc atgaagctca tagattgtca taccagcaag acgtttaatc
    28861 ataagctccc gtctgaactg ctcaaaatag agaagattca tggaatcaaa taacaagata
    28921 gccgtagaca gggtcaaaat cgtcccaata aggagagagt aaaactccac ccgtttgtca
    28981 tttagtacct tggcaaaaaa tagctggctt tttaccaagt aagaaacctt gtgatacaga
    29041 ccttgctctt ttagagctgt gatgctgtct ccatatcgat ctagtttcaa ggcactatta
    29101 gccgtagttc cccacaacat ccctgcaaca ggtcttgttc cagtcgcttg cggcgttaga
    29161 actacaataa tcggatcttt taggtactgg cgtgttgtct tgtacccatc attatagagg
    29221 aaacgttcct gtcctgtttc tgtaaaagct agccttacct gtgggaagta gtgtttctga
    29281 ctcgtcactt ctacactttc agatgaaaag ttttgcaggt aatctgtaaa caatccttgg
    29341 tagtaggcag actgctctcg caagctctca ggcaagatca gcccaaactc tccctcagac
    29401 aagttttgca tcttgtcaat aaactctgaa gaaacggtaa tcttttcttc tatcagatag
    29461 cgcggcgaga catagataac attccctgac ggtatgtagt cactgagacg attgccatct
    29521 aggtccactt ctgctccatc tgagaaattg tcaacattgc tcataatata aaaagagtct
    29581 gtattggcta accgttcttc agtaaatgtc tgccactcac gattatcctt acgcgttcct
    29641 tcttcatcgg caaatgcact agaccaacca aaggatagac ggtaacggtc tgaggactgg
    29701 ctccatttat tgctagctct ctccatttcc tgcatttcac ggtagtgggg taggagagct
    29761 gtcgcactcg atccgaccac caatacagct aagagttgcc ccaccatcat caatgtcatc
    29821 atccgtttga gagggagttt ccctttcaat agatccacca gactattttc ctgtaaacca
    29881 agtagatgga cgacacttag taaggtagaa atccctgcca aggtcaatcc ataaagtaga
    29941 agagcaatga tgaccagttg caccgttgcc ataaacaagg caccttgata ccagagaatc
    30001 cccaatccca aaagactgga taccagcact gagcagataa gctgtctcac atcttctaac
    30061 actggtctga gagcaattcc aaacaagctc tcaccagcta ttaagcgaat ccctgcctga
    30121 cgaagggatt tgatccgata aatcagggtc agactcataa aggtcagcag aaaaatagct
    30181 aaactcagta gcaccatagg ggtggccgtc aatagtaaga ctatcgaaaa tggatcttct
    30241 ccattcgaaa caaagccttg atagccaagt tctttcaagg tggtctgtaa gctcactcca
    30301 tccaaacttc ctgacacaat caagtaagac cctaataaat cactcgtttc tgcactctcc
    30361 ttggaggaaa tggtcagacc ttctggaagc tctccctcac cataagtggc ataggtaaag
    30421 cgtgtttctc cagcttcatt tggctcaacg attcgacggg cgatgagact attatgctct
    30481 accgccaagt gactcaaagc ctctgtcacc tcttcgcgac tagctactgg ttgatttgtc
    30541 atcattcctg tcacatcaac agaaggatag ttaccaaata aaatctctta tcgatggtca
    30601 acggaagtta tcaccatata aagcgatacc aataccattg aaatcaaaat aaataaacgt
    30661 ttcatcattt cctccaaaaa gttacttggt cacttagtga gaaggtgacc aagtaaagct
    30721 aaacaatagg gctataccat tctaaacatt atagtagaat gcaactttct ctccccaaga
    30781 agtacgaatc catgcacgag aagtccctcc agcaccagca tatcccttgt cagatgaacc
    30841 agtttttctg ctaacaactg ttgaactatg gtattttgaa gggtgatagt agtttgagta
    30901 agcaccaccg ttacctgaac cataagtcca aacaccacct tctgggtatt gaacggctgc
    30961 agaggcaact gctacacttc cacctaatgt tgctgcaatg ctcgctacaa gagctaattt
    31021 tttaactgtt tgtttcatat tgatactcct ctatttttga ttacaagatt gttgagttac
    31081 ttgctcctct ttccattatc aacgttgata ctattatatt accaaatgat attctaaaat
    31141 tatttgtttt tctctaaatt gtcaaaatag caccctgaaa tgaactttta tagtcattat
    31201 actccatctt tatcacacta tcaaggacat tttt
//
LOCUS       DAVKJD010000015        29871 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKJD010000015 DAVKJD010000000
VERSION     DAVKJD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29871
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.015"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(50..806)
                     /locus_tag="VW157_000754"
                     /pseudo
     CDS             complement(50..806)
                     /locus_tag="VW157_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(945..2567)
                     /gene="groL"
                     /locus_tag="VW157_000755"
     CDS             complement(945..2567)
                     /gene="groL"
                     /locus_tag="VW157_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9552088.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNHPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2583..2867)
                     /gene="groES"
                     /locus_tag="VW157_000756"
     CDS             complement(2583..2867)
                     /gene="groES"
                     /locus_tag="VW157_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9552089.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(3023..3418)
                     /locus_tag="VW157_000757"
     CDS             complement(3023..3418)
                     /locus_tag="VW157_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9552090.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3496..4257)
                     /locus_tag="VW157_000758"
     CDS             complement(3496..4257)
                     /locus_tag="VW157_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU9552091.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMVKVNQGHVINMGSTAGIYAYAGAAVYSATKAA
                     VKIFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMATQQATGFMVHKK"
     gene            complement(4290..4916)
                     /locus_tag="VW157_000759"
     CDS             complement(4290..4916)
                     /locus_tag="VW157_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9552092.1"
                     /translation="MIFTYNKEYVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNVRVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPCELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4932..5249)
                     /locus_tag="VW157_000760"
     CDS             complement(4932..5249)
                     /locus_tag="VW157_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9552093.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5246..5545)
                     /locus_tag="VW157_000761"
     CDS             complement(5246..5545)
                     /locus_tag="VW157_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9552094.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5830..6021)
                     /locus_tag="VW157_000762"
     CDS             complement(5830..6021)
                     /locus_tag="VW157_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEU9552095.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGAMIANDWVKDNGK"
     gene            complement(6182..6856)
                     /locus_tag="VW157_000763"
     CDS             complement(6182..6856)
                     /locus_tag="VW157_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552096.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(6862..7308)
                     /locus_tag="VW157_000764"
     CDS             complement(6862..7308)
                     /locus_tag="VW157_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9552097.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKSPRLIF
                     YKGTGEYYLDLSEILFFETEGSKIYAHTQKNAYEVRLKLYELEAILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7422..7925)
                     /locus_tag="VW157_000765"
     CDS             complement(7422..7925)
                     /locus_tag="VW157_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9552098.1"
                     /translation="MKNYQEWYRNIRSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWNICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLNFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7922..8284)
                     /locus_tag="VW157_000766"
     CDS             complement(7922..8284)
                     /locus_tag="VW157_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEU9552099.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8350..10209)
                     /locus_tag="VW157_000767"
                     /pseudo
     CDS             complement(8350..10209)
                     /locus_tag="VW157_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(10248..10697)
                     /locus_tag="VW157_000768"
     CDS             complement(10248..10697)
                     /locus_tag="VW157_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9552100.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIDQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(10821..11537)
                     /locus_tag="VW157_000769"
     CDS             complement(10821..11537)
                     /locus_tag="VW157_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9552101.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12418..13833)
                     /gene="ply"
                     /locus_tag="VW157_000770"
     CDS             complement(12418..13833)
                     /gene="ply"
                     /locus_tag="VW157_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9552102.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13844..14176)
                     /locus_tag="VW157_000771"
     CDS             complement(13844..14176)
                     /locus_tag="VW157_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552103.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(14247..14855)
                     /locus_tag="VW157_000772"
     CDS             complement(14247..14855)
                     /locus_tag="VW157_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552104.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14868..15326)
                     /locus_tag="VW157_000773"
     CDS             complement(14868..15326)
                     /locus_tag="VW157_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9552105.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15451..15850
                     /locus_tag="VW157_000774"
                     /pseudo
     CDS             15451..15850
                     /locus_tag="VW157_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(16615..16875)
                     /locus_tag="VW157_000775"
     CDS             complement(16615..16875)
                     /locus_tag="VW157_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552106.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFFDG"
     gene            complement(16934..17485)
                     /locus_tag="VW157_000776"
     CDS             complement(16934..17485)
                     /locus_tag="VW157_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552107.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(17475..17672)
                     /locus_tag="VW157_000777"
     CDS             complement(17475..17672)
                     /locus_tag="VW157_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552108.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(17676..18260)
                     /locus_tag="VW157_000778"
     CDS             complement(17676..18260)
                     /locus_tag="VW157_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552109.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKKFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGDKANMDVVLVFSKLSDGEKKQLLQARVPFVDFKG
                     NLFFPTLGLVLNANDTEVPKELIPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(18658..19083)
                     /locus_tag="VW157_000779"
     CDS             complement(18658..19083)
                     /locus_tag="VW157_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552110.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEAASIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILVEAFKA"
     gene            complement(19212..19595)
                     /locus_tag="VW157_000780"
     CDS             complement(19212..19595)
                     /locus_tag="VW157_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552111.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQFASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKKEMVLG"
     gene            complement(19617..19823)
                     /locus_tag="VW157_000781"
     CDS             complement(19617..19823)
                     /locus_tag="VW157_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552112.1"
                     /translation="MDLSNKAPNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(19810..19971)
                     /locus_tag="VW157_000782"
     CDS             complement(19810..19971)
                     /locus_tag="VW157_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9552113.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMITKMEKG
                     ERNITNGFK"
     gene            complement(20317..21273)
                     /gene="lytA"
                     /locus_tag="VW157_000783"
     CDS             complement(20317..21273)
                     /gene="lytA"
                     /locus_tag="VW157_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9552114.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(21649..23019)
                     /locus_tag="VW157_000784"
     CDS             complement(21649..23019)
                     /locus_tag="VW157_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9552115.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFAFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPRFKHFKWNKSLYVDLLEQGLAMGLMSSIVSIGSVILQSSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIAQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(23325..24491)
                     /gene="recA"
                     /locus_tag="VW157_000785"
     CDS             complement(23325..24491)
                     /gene="recA"
                     /locus_tag="VW157_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9552116.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            complement(24546..25802)
                     /locus_tag="VW157_000786"
     CDS             complement(24546..25802)
                     /locus_tag="VW157_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9552117.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(25887..26903)
                     /locus_tag="VW157_000787"
     CDS             complement(25887..26903)
                     /locus_tag="VW157_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9552118.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVVAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(26911..27429)
                     /locus_tag="VW157_000788"
     CDS             complement(26911..27429)
                     /locus_tag="VW157_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9552119.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(27419..27862)
                     /gene="tsaE"
                     /locus_tag="VW157_000789"
     CDS             complement(27419..27862)
                     /gene="tsaE"
                     /locus_tag="VW157_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9552120.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(27948..28568)
                     /locus_tag="VW157_000790"
     CDS             complement(27948..28568)
                     /locus_tag="VW157_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552121.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(28951..29814)
                     /locus_tag="VW157_000791"
     CDS             complement(28951..29814)
                     /locus_tag="VW157_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9552122.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
BASE COUNT         8921 a         6653 c         5144 g         9153 t
ORIGIN      
        1 aaatcgattt ctaacaatgt tttagaagta aaggtgtact attctagttt caatctacta
       61 taaatttgat atgagaatag tctttctcaa catccttacc caacaagaat gctgtagcgt
      121 ccttcttaac ttgaaccagg tcaatattct gggcttgggc tgcatagacg cagccacaat
      181 aacattgacg atagatatca tagctcctca cacatctcta ctgaacgttt gtagccttga
      241 tttttcttga aatcgttggg aagatagtgg gtcgtgtaaa ttttttgcac atcgattccg
      301 atgctgttga tggtttgaga attcttatga ggactgatgg tcaaggctga accaaagtag
      361 tcaaagccca agtccatagc cacttgcgct gttttatcca gtcggtagtc aaaacaaacc
      421 ttgcaacggt cgccaccttc gggctcctcc tctagtcccc taactagttt tcggtattaa
      481 ttgggttcgt agggagcttc taggtactga accgtatttc ctgtccgctc attaaaatca
      541 ctaacaaatt tcttgatgac atagacccgc ttatggtatt ctaccttggg atggatatta
      601 gaattggcaa aatagatggt cacatctgca tacttggtca aatattctag tgtataggta
      661 ctacaagggg cacagcaaac atgcacgaga atggttggcc gctgctcatt tttctcccat
      721 gcttgtacca ttttctgcat gacacggtca taattaatct tctgattggg gggcatcttg
      781 cacagaattt cttctacatc gatcaggttc ttctcctttt tctagtctta ttttatcata
      841 taagttagga gagctcaaat caatttagaa gtgaatatta taaaaagaag ggttagtcat
      901 tccctccttt tgtgtttttt tataagttgt tttctataga atgcttacat catcccgccc
      961 atcatgcttg gatccattgc tggagctggg gctactggtt ctggtttatt ggctacgact
     1021 gcttctgttg tcaaaatcaa gctggctaca gatgctgcat tttgtagggc tgaacgactc
     1081 actttaactg gatcaatgat accttgatca atcatgttaa cccactcgcc agttgctgcg
     1141 ttgaatccta taccaagctc agcatttttc aaacgatcga taacgataga tccttcaaat
     1201 cctgcattgt gagcaatttg acgaacgggt tcttccaaag cacggagaac aatattacgt
     1261 cctgttgctt catctcctgt caattccaag gtagcaacag ctggaatcac attggcaaga
     1321 gctgttccac cacctgcaac aataccttct tcaacagctg cacgagtagc gttgagggca
     1381 tcttcaatgc ggagtttcat ttctttcaac tcagtttcag ttgcggctcc gaccttaata
     1441 accgctacac cacctgacaa tttggccaag cgttcttgca atttttcacg gtcaaattca
     1501 gaagttgtag tttcgatttg agacttgata accgcaacac ggtgagaaat cgcttcagga
     1561 tttcctgcac cttctacaat aaccgtgcta tctttgtcca cggtcactct cgctgcttga
     1621 ccaagagctt caattgtcgc atctttcaac tcaagaccaa ggtcttctgt gataactgtt
     1681 ccgcctgtta agatggcgat atcttcaagc atagctttgc gacggtcacc aaaaccaggt
     1741 gccttgactg ctactacgtt gaaggttcca cgaatcttgt tcaaaacaag agttggaaga
     1801 gcctcgccat ccacatcatc cgcaataatc aagagtggat gattgctttg gagaatgctt
     1861 tccaaaagtg gcaagatttc ttggatattg gaaattttct tgtctgtaat caaaatgtac
     1921 ggattttcaa ggtcagccac cattttttcg ctatctgtca ccatgtactg tgaaaggtaa
     1981 ccacggtcaa actgcattcc ttctacgact tcaagctctg tttccatacc acgtgactct
     2041 tcgatggtga tgacaccgtc tttgccaact ttttccattg cttcagagat gtactcacca
     2101 actttttcag aacgagaaga tacggctgca acttgagcga tagcttcttt attggcaaca
     2161 gggatggcgt tgtttttcaa agcttcaact gctgcggcaa ctgctgtttc aatcccacga
     2221 cgaataccga ttggatttgc acctgctgtg acgtttttga ttccttcacg gacgatagct
     2281 tgggtcaaga ctgttgcagt cgtagttccg tcacctgcga tatcattggt tttagaagct
     2341 acttctgata ctaacttagc acccatattt tcaaaatggt cttccaattc gatttctttg
     2401 gcaatggtca caccgtcatt ggtaatcaag ggtgaaccga atgacttttc aagaacgaca
     2461 ttgcgacctt ttggtcccaa ggttacttta acagtgtctg caaggatatc gacaccacga
     2521 accatggctg aacgggcatc tgatgaaaat ttaatttctt ttgacatact tactttctcc
     2581 ttctattcct caatgattgc caaaatgtta gcttcgccta cgatgatgta cttttcatcg
     2641 ccatctttga catcaagacc tgcgtgggct tcaactaaga cacgatctcc agttttaaca
     2701 cttggagcaa ccaagtcacc gttcaaggta cgaacacctt gtccagtagc cacaacttga
     2761 gctgttttgg ttttttcttg ggctgagcct gcaaggacaa agcctccaac agtttgttct
     2821 ttttcttcta tttttaagac cacacggtcc cctaatggtt tcaacatctg atttcctcca
     2881 taatgagata gatattatta gcactctttg aacttgagtg ctaattcata gttctattgt
     2941 atcacttagt cagaaataat caagaaaaaa gtctgacttg ctcaagataa aaagcctgag
     3001 accaactcag actttttaat tcttaaaatg gcaattcttc ctcttccaag accaaatctg
     3061 ccaaatcttg gcctgcatta ttttcacgca tggcacgttg agcacgactt tctaagagtt
     3121 ggaatcctgt cacaaggact tcggttacat agttcatctg accatttttc tcaaagcgac
     3181 gggtacgcaa ttctccatca acggaaatga gactaccttt ggttgcgtag cttgccaaag
     3241 tttctgctag tctgccccat aggaccatat tgacaaaatc agcttcacgt tcaccgtttt
     3301 ggtctttgta acgacggttc acagcgatag ttgctcgcgc taccgacttg tcattgttgg
     3361 ttttgtgcaa ttctggtgta gacgttaaac gcccaatcat gataacttta ttatacatat
     3421 tttcttcctc ctacttatct attcgtagga aatcaaaaaa aattacagaa atttgtaact
     3481 tttcgagaaa attttttatt ttttatgaac catgaaacct gtcgcctgtt gagtggccat
     3541 aatggtcata tctgtaatct gaacacggcg aggctgactg gtcacataga ctactgtgtc
     3601 tgcaatatcc tgagcttgca aggcttctat tccttggtaa acggacgcag cccgctcttt
     3661 atcaccatga aaacgaacag ttgagaaatc tgtttcgaca atcccaggct gaatggttgt
     3721 caccttgata tccgttgcga tggtatcaat tcgcagtcca tccgaaaaga tcttaaccgc
     3781 tgccttggta gctgagtaaa cagcggcacc agcataggcg tagattcctg cggttgaccc
     3841 catattgata acgtgacctt gattgacttt taccattgct ggcaagaaac agcgagtaac
     3901 cgccatcaga cctttaacat tggtatccaa catggtcaac atatccaact cctcatagtc
     3961 ttgataggga gccaagtcaa gagccagtcc ggcgttgttg accaagatat caatcctccc
     4021 tatcgtttct agaatatcag agcaaacagt cttcaccatg accatatccg tcacatctag
     4081 tggaaaagtc cagacggttt gatttggaaa gcttactgca aactccgact tgaggatttc
     4141 taatctgtct atccgtcgtc ctgttagaac gacatcctca ccctgctcca gataagcacg
     4201 cgcaatcgct tcaccgattc ctgaggttgc tcccgtaatc actacatttt ttgccatctt
     4261 atttccttct atcagatgct gacaagttcc taggcagtcc agtgtttcgc tgggtcgaac
     4321 ggtgttccga caacttggtc ttctgataat tcaagcaccc cacgtttttg cggagcattt
     4381 ggcaaatgca attcacaagg actacacatc atcccaaaac tcttttcacc acgaagttcg
     4441 cctgggaaaa tgaaattccc ttttggcatc atggcaccag gaagggccac aatggttttc
     4501 aacccaacac gtacattggg agctcctgca acgatttgca ctatcttgtc acttgcgact
     4561 gcaacttggc agatgttgag gtggtcactg tctggatgag ctaccatctc gacaatttca
     4621 ccaacaacaa acttaggttc cttatcatta acaatttctt ctgtaaaacc ttccgcctgt
     4681 aattcttggt tcaaacgagc gacttgctca tctgataaaa agacttgacc gcgctctgca
     4741 atttcaaaca agctcgaaac ctcaaaaatg ttccaagcta ctgtttcccc attttctttg
     4801 aggaaaacac gggctacctt gcctttgcgt tccacattca gtttggcatc tccgctattt
     4861 ttcacgatga ccataaggac atcaccgaca tattctttgt tatacgtaaa aatcattgtt
     4921 tcctttttct cctatttcaa tcctgctaaa aaatcgttaa tttgctcctt acttttacgg
     4981 tcgcgattga caaaacgacc gatttccttg tccttttcta gaacaacaag gctaggaatt
     5041 ccgtaaacat cccagagttt ggccaaatcc atatactgat ctcggtccat tcgaataaag
     5101 gtgaactctg gattggtctc ttcaatctct ggtaaggcag gataaatata acgacaatcg
     5161 ccacaccagt ctgccacaaa aaggaagacc ttcttgcccg ctttttccac taaagatgct
     5221 aattcttcta aacttgctgg ctgtatcata agacttcctc ctcatagact aggtcttcat
     5281 tttcatagac aaaggtataa tgacggccat cctcaaaaat gacgccacca accaagctct
     5341 ccagactgct ttcgtaaact tgaacataaa gggtcgcaat ttcccccatg tcggaaaaat
     5401 ggtctcgcac aatctctgtc aactcttcct gagtcttcat gagcttacgg tcatctgcaa
     5461 cttttttcgt agcaagagca aggcttccga tacctagcag agccaagcct gccatccaca
     5521 tttttttagc tttcatacca ttcattttaa cacaaaaaag gcttcaggac aaatgaggaa
     5581 gcagcagaaa agcaagtaaa aagcctcttc ctttaaggaa aaggacttct tatactaaga
     5641 gcaaactaga aagctagccg caggctgctc aaaacacggt tttgaggttg tagataaggc
     5701 gacgttgacg tggtttgaag agattttcaa agagtattat tcttattgcc aggcacctaa
     5761 gttgccaacg tagtaaccat caggtgtgta ggtattgcga agcatcttac ctgatgaagc
     5821 cagataatac tacttgccat tgtctttgac ccaatcattc gcaatcatgg caccagaaga
     5881 acttacataa taccattctc ccttgtcata aacccaagta ctgactttca tggttcctga
     5941 gcaattaaag gcaaaaaaac tgtccaatat cattcgtttt ttaaaagcat ttgacactac
     6001 atcatctacg ctgtagtaca tagatacatc aaacgtaaaa gccaaaatat cgaaaaatat
     6061 acaactttta aaggcagcat ttcatttttt aaagtctaac aaccatagaa ctgaaatcag
     6121 agcaaggcat caatttccta ctcttagtac gtctattttg atggttgaag tgaagatttt
     6181 tttatttaac gtagacaatt tccaacttcc caaaagccac atctccacgg atagtcaagg
     6241 ttttgctggt tggggctaca ggagcgtcag ccttagctgc accaaaggag gtttctactt
     6301 tcaaatctac acgccagtgt tgtggaacat agacggttgc atttccgaag gccacttcaa
     6361 tatttaaagt tgcaaaatca cctagcatct ctgcattatc atagtagatt ttagcatccc
     6421 caaaagcgac ttccacttgg tcatctacga gatcttgatc ttgcttatag aaggtcccgc
     6481 taccaaaagc gacttcctta tccgtgacga ctgttttttt cccattgtac caccattttt
     6541 tttcattcca gaacttactt gaatgcgtca gataaccaac accaagtacc accaagatgc
     6601 tagcccaaat aagagaatga ttggtaactg gtaacaagtc ataagcgtaa tttgcaatga
     6661 tgagcgccag taaacctgta aaaactgccg aagtgagatg acgtctaagg atggactcaa
     6721 tggacttata agcaaaaaaa acaataccta gtaaaggcca tattttacca tccaaagaag
     6781 gaattccaaa attcccttgc agcaagatcc aagctgctaa aaccaataaa acaataccaa
     6841 atgctttctt tttcatgttt tttacctcat gtttcttaga ttttctttta ggagggattg
     6901 gtaatgccgt gagacatgaa cctccttgtg cgtctgataa aaggaaatgg tgcccgttcc
     6961 tgaaaaggac ttgtccactg agtaaatctg acggatgttt gcgatcgttg acttggaaac
     7021 tcgattaaaa tagcgaggaa ggatagcctc taactcatag agtttgagcc gaacttcgta
     7081 ggcatttttc tgggtatggg cataaatctt gctaccttct gtctcaaaga agagaatttc
     7141 cgacaagtcc agataatatt cacccgtccc cttgtaaaaa atcaacctag gagacttgga
     7201 ttcttgcaag agccgttgca agtctgcaat ttcatctgtc aaagtggggg tcttgatgac
     7261 aatttcagtc tcctctaaat tgccgtcaat ctcaattcgt aacttcatca catctccttt
     7321 ctgactctac tatatcaaag ctaagttagg aatacaaggg caaaaaagca agtggtcaat
     7381 ttgaagcagt aagtggttgt aggactagct taacttacat gtcagaataa gaagaaaagg
     7441 aatccccaca ccagaccaca agcatagcca accaagacat ccttgggata atgaacaccc
     7501 cctaaaaccc tcaccaaccc cagcaaagct gaaaagttca agcatactaa gccaataggt
     7561 agactagcat gcagacaggc catggagatg atagttgccg aaaagacatg gcgactgggc
     7621 atcgaccgtc ccgaactatc cttatcaaga aggggacaga tattccaagt ttcataaggt
     7681 ctcgggtgat tgattttctt acgaaacagg gacaaggcca caaaacctga tgcaggaata
     7741 agcacataaa ccccaacttg ctgaccaagt cctagttgca ggtaagtggt gactagcaag
     7801 atcagataga ccaggggcat ggcgactgtc atcaaacgat tgaaactgcg taataggaat
     7861 aaaagaattg gacgactagt tagtctggat ctgatatttc gataccattc ttgataattt
     7921 ttcatttatg cttctcatac ttttctagaa cttgttgaga tgttttcccc ttggcaagcc
     7981 aatccactag cttgtccaag cgacgtattt cctgcatcag aggatcatca atttcctcaa
     8041 tacggacacc gcaaatcttc cctgtaattg caattcgttc agggacataa tatggtgctt
     8101 gtcgaaaaaa atctccgtaa gtaacatccc tatccagaca agcaagaacg tcactcactt
     8161 cataccctgt caaccaactg gtcacttggt gaactgactc tgcctttcca ccctttcgct
     8221 caacttttgc aatcaaggct aagtagacag cagcaaattt catattgtat aatttctgac
     8281 tcatacttct ctctttctat gtgtactagt ataaataaga aaaagaaggc cgtcaagcct
     8341 tcttttgatt tattcttctg cttcatcttc tgtaaattga ctattataca agtcagcgta
     8401 gaagccacct tgcgccatca gttcctcata gttgccttgc tcgatgatat ttccatcttt
     8461 catgaccaag atcaagtctg catttcggat ggttgacaag cggtgggcaa tgacaaagga
     8521 tgtgcgtcct tccatcaaac ggtccatggc tttttggatc aattcctctg tccgtgtgtc
     8581 aacagaagaa gtcgcctcat ccaaaatcaa aagcggtgca tccttaagaa gggcacgagc
     8641 aatagtcaat agttgttttt gtcctacaga caaggtcacg gtgtcatcca agatggtatc
     8701 atagccatct ggcaaggtca taataaagtg gtgaattccc acagccttac tagcttccat
     8761 cattcgttca tcactaatcc ctatttgatt atagatgaga ttgtctcgaa tagttccttc
     8821 aaagagccag gtatcctgca agaccattga aaaggcatca tgcacttctg aacgcgtcat
     8881 agccttggta tccacaccat caatgcgaat acttctctta tcaatctcat agaatttcat
     8941 caaaagattg acaatggttg tcttaccagc cccagtcggc ccaacaatgg caaccctttg
     9001 accagcatga gctgtcacag agaagtcata gtcttgaaca ttgacaccgt ccaccagaat
     9061 ttctcctgct gacacgtcgt agaaacgtgg aatcagattg accagagttg atttaccaga
     9121 acctgttgac ccaataaagg ccactgtttg accagtttct gctttaaagc taacatgttc
     9181 aataactgcc tccgaatttg ccgcatagcg gaaggtcaca tccttaaact cgacctgacc
     9241 tttgaagttt tcatcagtca gctgcacttg aacagggttt tggatagaag aatgcaaatc
     9301 taaaacttga ttaatccgct tagcagagac catagttcgg ggaagaacga tgaagagtgc
     9361 tcccatgaga aggaagccca tgacaaccta catggcataa gacatgaaaa taatcatgtc
     9421 actaaagaga ggcagacgcg ctatcggagc agcgtcgtta atcacatagg ccccaatcca
     9481 gtaaatcgct acactcaaac cacttgaaat ccccatcatg ataggattca aaatagccat
     9541 aagacggttg acaaacaaat tcaaacgggt caattcatca tttactgctg caaatttttc
     9601 attttgataa tcctctgcat tgtaggcacg aacgacacga atacctgtta aactctcacg
     9661 agtgatactg ttcagtttat ctgtcaaccc ctgaatcaag gactgttttg gaaaggctag
     9721 cgtcatcaaa acggtcgtca tcaggacgtt gacaatcact accacaagta cggcccagag
     9781 ccagtattct gaatgaccta aaatcttccc aatagcccag atagccataa ttgaaccacg
     9841 cgttaccact tgcaagccca tagtaatcaa catttgaact tgagtaatgt cattggtagt
     9901 acgcgtcaag aggctaggaa ttgaaaattt cttaatctct gtctgcgagt aatccaaaac
     9961 tcggttaaaa atatcacttc tcagcctact agtataagaa gccgccactc gggatgcaaa
    10021 aaatccaact gcaactacgg acaagaaggc aagaaaggac attcccatca tcatgcttgc
    10081 cggctgccac aactcatcta aattagtttc ttgactacct agcaaatccg taattttcga
    10141 gatataggtc ggcacttcca actctagata gaccgaaaag caagtaaaga gaatggctag
    10201 taaaatcatc ccccattctt ttctactaat tcttttggct aatttcttta ttctctcctc
    10261 ctattccctt gatattttgc ctgtagttga ccgagaacct tctcaaaaat cagtaattca
    10321 tcttcatcaa tatcttccat caactgcttg tctatgcgtt caaaaaaagc cttaacctgt
    10381 tgcatctgag aacgtgcttt gtccgtcaga cgaacaaact tagcccgctt atcaacagga
    10441 ctcgcctcca attccaccaa accattttgc actatacgct taaccagatt actagcaaca
    10501 gacttggtaa tattgagttc ctgatcgata tctttaatca agaccaagtc ttggtttttc
    10561 tcgcgattat ccaaaaaatg cacaacctga ccttgcggcc cacccataaa ttcaatgccg
    10621 caacgtttgg cttccttttg caccatcagg tgaatttgat gaccaaaacg cttaaagact
    10681 aacatcggtt tacccataat ctcccccttc taaataaaaa tagttctctg gagaataatt
    10741 aaatttctat gagaactatt ttcttgatta aaaaaatccc aagtgatttt ctcacttagg
    10801 atcatgttct ataggttaaa ttaaaaccca tctacgttcg tataaatctt ttggacgtct
    10861 tcgtcgtctt caagaacgct gtaaagtttt tcaaaggttt caaggtcttc gcctgacaat
    10921 tccacttctg actgaggaat catttccaat tcagtcactt ggaattcttc aataccagac
    10981 tcacggagag caacgatagc cttgtgaagg tcagttggcg ctgtgtaaac tgtgattgta
    11041 ccttcttgtg cttctacgtc atccacatcc acatccgctt cgagcaattg ctcaaagact
    11101 gcgtctgcat cttcacctcc aaatacaata acacctttgt tgtcaaagag gtaagaaaca
    11161 gaacctgaag cgcccatgtt tccgccgttt ttaccaaagg ctgcacggac attggctgct
    11221 gtacggttga cgttagaagt caaagtatcc acaattagca tagagccatt tggcccaaaa
    11281 ccttcgtaac gtccttctgt aaaggtttcg tctgtgtttc ctttggcttt atcaatcgct
    11341 ttatcgataa tgtgttttgg cacttgggct tgtttagcac ggtcgataac gaatttcaaa
    11401 gctgagtttg attctggatc tggatcacct tttttagctg ctacatagat ttctacacca
    11461 aattttgcat atactttaga gttggctcca tctttagccg ttttcttggc tacgatattg
    11521 gcccatttac gtcccattag gaatctcctt ttttcacatt ttaatcttta ttattataac
    11581 acaagttttt ttgattttca ctagaggaaa tggattttat tagcaaatca agctaggata
    11641 gcactttacc tgctaagatg gtcttacctt tctatcttta tcaacagaca ctcatccaca
    11701 ttcaaaaaac aaactagacc attatctgca aatagaaagt ttcagccaag tttgacaaag
    11761 tcagctcaaa ttactgtttg aagtttgtag atataagcga caaaaacaat catactgcac
    11821 cttttgttga cagtctactc cagacatatc atagttcaag taaatacttt gaaatccaac
    11881 agttcttata agcgctattg tattctaaga aatcaataga agagtttcta agcaaacctc
    11941 taatactcaa taaaaatcaa agagcaaact agaaagctag ccgcaggttg ctcaaaacac
    12001 tgttttgagg ttgcggatag ggctgacatg gtttgaagag attttcgaag agtataattt
    12061 acgtgttcct aagatggaga agttagacta gtacactggc acttctaaac cattgctagc
    12121 aattgatttg ttcatattta atttcatttt ttccataaat gggtattaga tataaacagc
    12181 aaaatatttc cgatacgtgt cattcttgaa tttccaatca tctaaaacaa gtaaaggata
    12241 atcaatcccc tgtatatcaa ggaattggct acccttttta cttttttaca cattctgttt
    12301 gatagattca ttttaacatc acgagcatac tccaatggaa atcgctaggc aagagataaa
    12361 ctttcagata tccgcagaga gatcatcgcc tctttttgtc gcaagcattc tcctctccta
    12421 gtcattttct accttatctt ctacctgagg atagagagtt gttccccaaa tagaaatcgt
    12481 ccgcttacgc actagtggca aatcggtttt ttcataaacc gtacgccacc attcccaggc
    12541 aagcccggta cactctctaa ttttgacaga gagattacga acattccctt ttaaaggaat
    12601 actagtggta aagtgagccg ttaaatcctg cccatttctg tcccaagcct taggagtcaa
    12661 gacttcctta ccttgatgat cataggataa ttcattccaa gtaatataat attgggcaac
    12721 ataggcacca ctatgatcca gcagtaaatc tccgtttctg taagctgtaa ccttagtctc
    12781 aacatagtct gtactatttt gaaaggtcgc aactacattg tcacgtaaaa aagaagttgt
    12841 ataggaaatc ggcaagcctg gatgatctgc tgtaaagcga ctgccttctt gaatcaagtc
    12901 ctctaccata tccaccttgc ctgttacaac tcgggcaccc gaacttgggt cgccccctaa
    12961 aataaccgcc ttcacttctg tattgtccaa aatctgcttc cactctgtct gaggagctac
    13021 cttgactcct tttatcaaag cttcaaaagc agcctctact tcatcactct tactcgtggt
    13081 ttccaacttg agatagactt ggcgcccata agcaacactc gaaatataga ccaaaggacg
    13141 ctctgcagaa attcctctct gttttaaatc ctctaccgtt acagtatctt gaaacacatc
    13201 tcctggattt ttaacagcat ctacgctgac tgtataataa atctgcttaa aattaacaat
    13261 ctgaatctgc ttttcgcctg aatggacaga gttaaaatca atatcaagag aattccctgt
    13321 cttttcaaag tcagaaccaa acttgacctt gagttgttcc atgctgtgag ccgtgatttt
    13381 ttcatactgc attctagctg ggacattatt gacctgacca taatcttgat gccacttagc
    13441 caacaaatcg tttaccgctc cgcgaacact tgaattgctg gggtcttcca cttggagaaa
    13501 gctatcgcta cttgccaaac caggcaaatc aatactataa gtcatcggag cacgatcgac
    13561 cgcaagaaga gtgggattat tctctaacaa ggtctcatcc actacgagaa gtgctccagg
    13621 atagaggcga ctgtcgttgg tagctgttac agaaatatca cttgtatttg tcgacaagct
    13681 ccgcttcttt ctttcgataa caacaaactc atcgggtagc tgattaccct ctttgatgaa
    13741 acgattttca atactttctc cctgatgggt caagagtttc tttttatcgt aattcatagc
    13801 tagtataaag tcatttactg ctttatttgc catcttctac ctcctaataa gttcctggat
    13861 tgagttgcat aaactcagac ttgttcagcg aaatcagccg tggttggact aagtgatcca
    13921 aaatttcctc gtacaattct tctgagacat tgcgtcgccg tctggctaga taagaagtcg
    13981 gaatgaccgt attatccaac ataaatacct tatctaagtc aatcaaggtt ggtcttgtaa
    14041 aaggattacg agctagatct ggctcttcta tcataaagtt cttgaccaaa cgtctggtca
    14101 agagagctgg tttgaaggtc tgatttttaa ccaactcttt gtttttagtc atgctgttgt
    14161 caatacagat atacatatga ttcttcacag ccaaatcgct actaatagtc ggaaaaggca
    14221 aataaagagc tacaacatct cctctcttaa tcaagcaaga gcaccccctt ttctcctaat
    14281 gtaacataga caggattgac caagtcttct gattgactca gaatttccaa agtttgagtt
    14341 tggcgcgctg tcaatttagt agcatcttgt ctcttcaata caaaatgctt gtcgccaata
    14401 accttgacaa tataatcctt ctccaaagct gactggtaaa tccacatcag atgttgtctg
    14461 tcctgagaac tcaagagaga aggattttca agcctcccga tagtctgata aaaatcaaaa
    14521 acaggagcta actcctgcca atctgattgg ctagttgtca aggctagaaa aagggctttg
    14581 cgagctgata cttcttggtt agccttgaga gttactttcc cctccaagtt ttttagaaat
    14641 cgggaaactc cagaaagcaa atttttctct aactgcgaga aataaaaacc tttcgttccc
    14701 agacataagt ctttcatgtc gctttctcta gcaaataaga gctcaaacat ttgatagtaa
    14761 aagaaaaata tctggcactg ggtcgcgctc atcttttcct tatcggcttc tttttttaac
    14821 cagagcaagg gcgacaggta gctggattga gacatttcct ctacctccta ctctttttta
    14881 actggagcat ctgcactagc tgccacttct tttgactgga tactttccca ctggttaatc
    14941 tcctctgaga taagaccttc gcatgtcttg acaaataggg caaaagcctt ggtctttcct
    15001 gcatatttct ccgtttggca ttgatagagg aatttttctt tctccaggag ttgcgcagtt
    15061 ttttggtaag aaatccaatt ttcctttgca ttatacaaat tgataatccc ctcacacagc
    15121 aagccgagac tggataaggc aaccgaaatc aaacggtagc gatcacctgg cataggaata
    15181 gcacaaaaga cagctatgag gaaacctgcc acgatttctg ttatttttaa taccttatag
    15241 cgcctacgat gttgaacgct tttctttaaa aaatgagcta tctgtacgtc taatcgctct
    15301 gtcaggtaca tttcttctgg cgtcatattc gtaactcctt tcatttactt tgataatcat
    15361 agtataacat atttgaatat caaagtatag taaaaactgg actataactt attagacttc
    15421 ctacgaaaca aaatatggta tagtagttct atgaattatg aagcaagtaa acaactaact
    15481 gatgcacgat ttaagctcct tgttagtgtt cagcgtacca cttttgaaga gatgttagct
    15541 gtattaaaaa cagcttatca aaaaagtcgg acaagctggt aaaatcttgg ctgacagtgg
    15601 ttatcaaggg cccatgaaga tatatcctca agcacaaact ccacgtaaat ccagcaaact
    15661 caagtcgcta atagctgaag ataaagccta taaccatgcg ctatctaagg agagaagcaa
    15721 ggttgagaac atctttgcca aagtagaaac gtttaaaatg ttttcaacaa cctattgaaa
    15781 tcatcgtaaa cgcttcggat tacgaatgaa tttgattgct ggtattatca atcatgaact
    15841 aggattctag ttttgcagga agtctattat atatatgcac agacatatga aatctttttg
    15901 atacagcctt ctttttacat agagagacag cagttatgat atatttatcc attcacaaca
    15961 tcttctgtac caatactaca ataattggta cattttgatt acacaatcgt ttcaaaaaat
    16021 atgatggaag actgagtatt ttatagtaat tcccttccta tcaactagat caaccagttg
    16081 agtgttgaat gttgaaacac cctagcctca gcaaaggact atgatcagca tttcctagcc
    16141 aataggacgc tccaaatcac ctattctaag tccatcacta gcttggtcag taagatacag
    16201 actgcaaggc aagactgacg tggttgatga gattttggaa gagtattaca atcatagatt
    16261 caccagtaga cttgaccgca gatttaacta tagtagattg aaactagaat agtacacttc
    16321 tgcttctaaa acattgttag aaatcgattt gactgtcctg atcaatttgt cctgttctcg
    16381 tttcatttta ctatactaaa aaaccacctg aaacaaatca agtgactaga ggataaaaac
    16441 aaagactggg caaaaagttt tgaaaagcaa aaaacgcata atatcaggtc ttgaaaaacc
    16501 ttgatactat acgtttgatt gtgggaagat tgacttcatt ttctcctgaa attgaagttt
    16561 tttctagttt ccaggtatag acgaattcaa tatagccatc tcgctacaat tattttaacc
    16621 atcgaaaaaa tttttcagaa tttcaaaaat ttcattttga tccaatgaaa tgtcattatt
    16681 ttgaagaata gttgacttca caggctctaa ttctatcaca aagcggtgca tgtctgcttt
    16741 taccgcacta cttgatgtta tagccgataa cttttcatct cctagcaagg aagctgtcaa
    16801 acgggtaagg tcattcaaat gctttttaat ggacttactt aaaccttgag cacctgtggc
    16861 agagcgctcc ctcatatcca gccaagcttt agccttaaaa ataattaata ctcaatgaaa
    16921 atcaaagagc aaactaggaa gctagttgca ggctgctcaa aacactgttt tgaggttgta
    16981 gatggaagct gacgtggttt gaagagattt tcgaagagta tagaccacaa ttactcaata
    17041 ccgaataccc ctgaatggtt tcttttacat gcaccaatat attataataa tcttcatcca
    17101 ataataaggc tgataaacta gcatcttggt caaaatataa gggaatttct cgaccgtcct
    17161 tctttaatgg atattctggt aagatactaa atagttcaat catagaagga aactcagaat
    17221 tagttgttgt aaatcgaaaa agctgcgctt tctcatcttt ctgacttcct tgatactctc
    17281 ccaattctaa aaaatgattc aaggtagtat aaaattcctt attttttact tcatcaatga
    17341 tgaccatatc ataatctttt gttgtgcgac ttttaaatcc ttgcgaatcc aatacgatag
    17401 aggtagcagt tcccccaatc agaacataat agttctgaaa atccgcaaac atttcttgaa
    17461 aaataacttt cgtattagct ggcatcatct tctcccagat actgtaatat catattttct
    17521 agtgcttcac tctcttcctc tatacgtggg tcatcatcat cttttaaggt caaataaaga
    17581 gaaatcggat ctacaaattg tttatcatga ttatttttaa aatcattcca aaactcagat
    17641 acaaaaggac gatatttcca tatctctagc atctttcatt ttaaaacatg ctgagaaagt
    17701 ggcaaggata actgattgaa ttttctctgc catatgacat agctagtatt ttcatccgtt
    17761 tcagctaaaa aagttgaatg cgacaaagca taagcaccac catatagaag gttagaaaca
    17821 gattttatct gctttatatc gccatctggc aataaaaagg caatccacgt taattgttcg
    17881 ctaggtatta attccttagg gacttcagta tcattcgcat tcagtactag tcccaatgta
    17941 gggaagaaga ggtttccctt aaagtctaca aacggaactc tagcttgaag taattgcttt
    18001 ttttcaccgt ctgataactt cgaaaacacc aaaacaacat ccatattggc tttatcaccc
    18061 atagtgcgag cctgagtaac aaatgaactc aaactccccc ttctcttttc tttaatcaat
    18121 aaaaatgatt ccttatgaaa agtcatttga gtatactcaa acttttttac aaaactgatt
    18181 ggtaaagtca aagtcagttc ctcaatactg acatcaacat aaaaggattg aaaaattgac
    18241 attatctttt tgacattcat gttttttcct tattctatca tttttattat attttatcat
    18301 tcggtataat aaaatataag ttttttgata aaataaccaa aaactactcg ctatcccctc
    18361 tcacattacc ctacatatcg tttgacatgc gactgatagt tcaggaaaac ttcaaggaga
    18421 acttttctct catccactat gcaggactta ctatttcact tctactccta taggctcaat
    18481 ttgacatttt tctttttcga attgctcata tgcctcctga atagtgagtt cgatttatcc
    18541 aaatttattt ttgtctattt tgatgaatac ttccacgaag aatgaatttg caccaatttt
    18601 ccttctcttt ttaagcatat tattccagat atgattgccc tcttgttcag taataaatta
    18661 cgctttaaac gcttcaacca gtatatctcc tgttgtcata tgttctaaag aaaattcttc
    18721 tacatatggt ttaacatctc ttaggttatt acttcctaat atcccattat gctttttcgc
    18781 taagaaaata gaagccgctt ctcccttacc aataatcttg ttactatcat gatttcttgt
    18841 taaatctcta tataatgcgt attcttcagt tccaatgtct atgctcacaa tctcagctga
    18901 acccttagct accaactgat ctatcctaga ttttaaatgg ggaattgtag gtatattgat
    18961 ttcatcatac acctcttgtg gaataacaat tttacccgaa tagagctttt ctaaaagatg
    19021 ttcagtgcca acccataaaa atgctgaaat acaatctgca tcaataaata cacgactagt
    19081 caaacgacaa ctcccccctc ttcatatagc ccatatacaa tatcatatct gaaagcatct
    19141 agtaacagtt cctcatactt cccttgcgaa attctgttat tttctaaaag ttgttcagtt
    19201 gaattaatat attatcctaa taccatttcc tttttacttt ctgacaaagg acgatataaa
    19261 cttgtatcat agcctaatct tgaagctgtc tctataacac taatatccat atttttaatt
    19321 tcttctgcat caaggtatcc atcatttctc aatctatata acatagcttt atgactgata
    19381 ccataaaact gacccaattt tataatatct tctacttcaa gatgagttct attggcattt
    19441 tctctaattt cctcaaccat cctatacagt gaagatggga aaattaaaaa ataagaagca
    19501 aactgatccg cttttctttc agtttcatct ccttcaccaa tcaagataag actgactgaa
    19561 ctcttcttca cctcatcata ataaagatga tacagttcat gtgctaaaga aaatctttac
    19621 cttcctaatg acatgtctga attgactgca atgagactga actgagttcc tttataacag
    19681 actccgctaa tattctttcc gagtccataa aataccagcg tcaaattttc tatcttttgt
    19741 accaatttaa aaatatctat cggcgattca ccatcagctc ccaacttttt tctaagattt
    19801 ggagctttat tacttaaatc cattcgtgat attcctttca cctttttcca ttttggtaat
    19861 catgctttga ttcaaagaca aatactcagc aatcttatct tgagtaagat ttgaagatat
    19921 tctaagttct tttattcttt ttccaacatc acatacattt atcatattca tacacctctc
    19981 ataaaaagaa tagcacactc ttgtcataat tttttaaata aaaaaattat gacaaaacaa
    20041 ggaagcaatt tattgatgct gcttaaaaat ctaaaattga tgagttccta tcaaaaatcg
    20101 tatcttcaac ctcaaaacag tacttaaagc tatccgactc ggtttacatt gtcaaattta
    20161 gattttattt gagcataact ttctagtttg ctttttgatt tttgtttaat atagtagcaa
    20221 aaattgaagg aaatatctcc acaagaaaac gcatactatt aagctttttc aagacctaat
    20281 aatatgcgct gttctgattt gaaagacatt ccattattat tttactgtaa tcaagccatc
    20341 tggctctact gtgaattctg gcttgtctgc cagtgttccg tctggtttga ggtagtacca
    20401 gcctgttccg tccgctgact ggataaaggc atttgatacc atggcgcctt ctttagcgtc
    20461 taagtagtac caagtgtcct tgtacttgac ccagcctgtc ttcatggcac cttcttcgtt
    20521 gaaatagtac cacttatcag cgattttctt ccagcctgta gccatttcgc ctgagttgtc
    20581 gaaccagtac cagttgccgt ctgtgtgctt cctccagcgg tctgcaagca tatagcctga
    20641 actgtcaaag tagtaccaag tgccattgat tttctcaaac ttgtcttttg gataagagcc
    20701 gtctgaatgt acgtaccagt agccagtgtc attcttctgc cagcctgttt caatcgtcaa
    20761 gccgttctca atatcatgct taaactgctc acggctaatg ccccatttag caagatatgg
    20821 ataagggtca acgtggtctg agtggttgtt tggttggtta ttcgtgcaat actcgtgcgt
    20881 tttaattcca gctaaactcc ctgtatcaag cgttttcggc aaacctgctt catctgctag
    20941 attgcgtaag agttcgatat aaaggcggta gtccgtcatg aactcttctt tggttgaatg
    21001 gctttcaatc agttcaaccg ctgcataggt ctcagcattc caaccgcccc caacgtccca
    21061 ggcaccatta tcaacaggtc ctacctgcat gatgcaaccg ttcccaacaa tgtgcgagaa
    21121 aaaacctaat tctgggtctt tccgccagtg ataatccgct tcattctgta cggttgaatg
    21181 cggattccca gttgagtgtg cgtgtacttg cctatatggt tgcacgccga cttgaggcaa
    21241 atctgttctt aatttactca cattaatttc catattctac tccttatcaa ttaaaacaac
    21301 tcatttttta caatccagaa ccagaaactc ctttatttct accttacaaa gaagacaatc
    21361 ttagtcacga ttaggcttgt agatagaacc tcaaaacgca ctattttgac actgtaaata
    21421 ggactgacaa ggtctgcatt ctatctacaa taacacccca gactaaaaag cttttcaaaa
    21481 gtatattttt acagtctcta catgtccttt tcataaatac tatactttat tatatcatat
    21541 aaaagaagtc aaaagtctgt taaactattt tcaacaccaa actaaagaag agaacacaag
    21601 ttttttcgat gttcactaga ggaaatggat tttattcagt aaatccaact aggattgcac
    21661 tttggttgcc aaaattgcct ttccttcttt tatcaaggga tgacggaata gtgagaagta
    21721 cagttgaact gtcatggcaa cccagataag aggttcacaa aggataacac ccttatatcc
    21781 tgcccaagga ataatcaaaa ccacaaaaac gatttttccg attagttcaa taaagctaga
    21841 aactagagga aggatcttct gccccaagcc ctgcaagcaa ttgcgataaa tcaacaagag
    21901 gctcaaaatg ggataaaagg ctgaactgat ttgcagatag agacttccat tttctatcaa
    21961 gtaaccatct gtcgaactag ccaagaagga aaccaaggct ggactggcaa aaaagaggaa
    22021 aatacaaaca aaaactgccc aggatatact taaacgactg ccgattcgaa gaccttgagc
    22081 aatgcggtca ggtcgcttag ctcctagatt ctgagaagca aaggtcgtca ttgatgcaga
    22141 aatagcggtc ataggaagaa gggcaaaggt cataatgcgt cgagctgccg tctgggcact
    22201 aataatcact gcaccaaatg tattaacaga agactgtaaa atcacactgc cgatagatac
    22261 aattgaactc atcaagccca tagccaaacc ttgctccaag agatccacgt acaagctttt
    22321 gttccatttg aaatgtttaa accgtggcaa gagttctggc acacttttac gaatataata
    22381 aaagcagaga accgctgata aaccttgtga aataatagta gcaagtcctg cggattgaac
    22441 tcccagatgc aattgcgtaa taaaatagag atccagaacc acattaacca aggcagagaa
    22501 aatcagaaat cccagggctg ctagactgtc accaatagac cgcaacaagc ctgcaaaaag
    22561 attataagca aagctgacac ctacacaggt cacaatcata gaaatatatt gataagattg
    22621 aggaagaatt tctgcaggag tatctaagta ttgcaagaga ggatacaagc caagaaagcc
    22681 cagcaacata actagaatgc tcaaaagagc acctaaaatc caggtggctg ctactgcttc
    22741 cttgatttta gtgaaattcc gagccccata ataacgagca atgacaatcc ccatgccatt
    22801 gccaacacca agtgtaaaac ctacaatcag gtcaaaaatc gctgtcgtcg ctcctactgc
    22861 agccaaggat tcttgaccaa gaaatcgtcc aacaatcaag acatcagcag tgttatagag
    22921 ctgttgaaaa atatttgata gcaagattgg gaaggcgaag cttaagagcg agggaagaat
    22981 cggaccatgt atcaggtcca ctgttcgttt tttattcata aagctattat atcagcttcc
    23041 tagaggagcg acaagaaaca gcaaactata tatagcggaa tgaaataagg gcagtcaaat
    23101 cgatttttaa caatgtttta gaagtagatg tgtactattc tagtttcgat ctattatagt
    23161 tggttgagtt tggaatagta cgctgtagct gctaaaacat ttctagaaat taatttgact
    23221 ttcctaataa agttgttcat atcttatttc aagctactat atataatcta aaaacttgaa
    23281 tcaaaaatcg aaaaagtagt ggatttctcc actgctttaa cagcttattc ttcaatttcg
    23341 atttcaagtt catcgcctaa gtcaagcgta acttcttcat tcactgcttc ttctttcttt
    23401 ggctcatctt ttttgttttc agtatcttgt tctgaaactt cttctccatc aatcaagcca
    23461 aatttagaac ggacttgctt atcaatttca tcaaagattt ctgggtgctc tgccaagtat
    23521 ttcttagcat tctcagaacc ttgcccaatt ttttcatctt tgtaagaata ccaagcccct
    23581 gcttttttga taatatccaa atcgcttgca atcttcaaaa gctcaccagt cttagaaatt
    23641 ccttctccgt acataatttc aactacggct tccttaaacg gtggagctac cttatttttt
    23701 acaaccttaa tcttagtttc tttaccgaca ttggtttctt tttgatcacc agttccctta
    23761 atttgtgtat taccacgaac atccaagcgg actgaagcat agaatttcaa agcacgtccg
    23821 cccggtgttg tttctggatt tccaaacatc actccaactt tttcacgcaa ttggttgata
    23881 aaaatggcaa ttgttttggt tttattgata gaggcgccaa gtttacgcat ggcctggctc
    23941 atcatacgag cctgcaaacc aacatggcta tctccgatat ctccatcaat ttccgcacga
    24001 ggaacaaggg cagcaactga gtcgactaca acaagatcaa ctgcacctga gtcaatcaat
    24061 tttcccgcaa tctcaagacc ttgctctcct gagtctggtt gagacaagag caattcgtca
    24121 atattgacac caagggccgc agcataagct ggatcaaggg catgttccgc atcgataaag
    24181 gcagcaatcc caccttcttt ttgcgcttgt gcaactgcat gaagggcaac cgttgtctta
    24241 ccagatgact ctgggccata gatttcgatg atacgtccct taggataacc acctgagcca
    24301 agggcaatgt caagagctaa agaacctgag ctcatcactt gcaccttttg ctccgcacgt
    24361 tcacccaaac gcatgattga tcctttacca aagtctttct caatcaattt aagagcgtca
    24421 ttcaaggcct tttcacgttc tgccccaaat ttttttgaaa tttcttctaa tttttttggt
    24481 tttttcgcca ttctattctc ctacattcta atgatcctca gacctatcta ctattatatc
    24541 aaaagttagt cacttaataa agccttgcga actaggttaa aggcatgcat aaccgcaatg
    24601 tgacgtacat ctgctcggct tctgcctcca atattaacct tgataacctc agttccttga
    24661 tcttgcgcca agcctatgaa gactgtccca actgggtgtc cttctaggct atctggtcct
    24721 gccactccag tcaaactaat tccaaaatca gactgggtct tgcttcgtgc ctgctcagcc
    24781 atcttctgag ctgtaaattc agacaccaca ccatgttctt ccaagttctt ggcaggaata
    24841 tccaacatcc ttgatttttc ctccaagcta taggtcacaa aaccaccctt aaatatactt
    24901 gaaactccag aaaaattcgc cacggtagct tggaaaagac ctgccgtcaa actctctgca
    24961 gccgcgatgg ttttcccttg ccttttcagt tcttctacca caatgctggc taaactagtt
    25021 tcttccccat aaccatagca aaagtctcgt aaagaaattc cttcgaaagt ctggcagtcc
    25081 aagatttgat tttccaagat atccagcgct tgattcgcct cttcttgact gctagccttt
    25141 gttgacagac gtagagtgac ttctcctgtc ttggcataag gggccaaggt aggatcgatc
    25201 tgattatcaa ttaaatcagc caaaatcgta accaactggc tctcgccaat cccaaagaaa
    25261 cgaagaactc gggaatacag cttgctccct gtcatcaact tgggtagaag ttggtttaag
    25321 accatgggtt tcaattcact tggcggacct ggaaggacga cataggtcac tccgtctact
    25381 tctaattttc ctccaacagc cagtcctgtt tcgtttggca gtggaatcgc tccttctaca
    25441 atttgagctt gtctttcgtt attcggtgtt cgggcatagt ctggtcgcag ggcaaaaaag
    25501 atatccaact tctcctgagc ctgaggatca aagactaatg ctttccctaa aaatttagct
    25561 agggtttgtt tggttaggtc gtcctcagtt ggccccaaac cgcctgtcaa aatcaccaga
    25621 ctgctacgtt gactggcaat ctcaagcaaa gacaagagac gaacttcatt gtctcctaca
    25681 gccgtctgaa aatatacatc taccccaatc tcagctagtt tttccgacaa aaactgggca
    25741 ttggtgttga caatctgtcc tgtcaaaatc tctgttccaa cagcaatgat ttctgctttc
    25801 atgtttcctc ctacctatct attcgtattt ttttgaaaaa atcgcaggaa ttttcctacg
    25861 attgattttt ttatttgtat caaaagttaa ttatcttcat caccaacagg tgctctgcca
    25921 aataaatctt caaataaaac cgcattggtt tcaagctgag taacttcttc ttgtcccaaa
    25981 gaacgtcgga gtatattttg catttccaac atatgtgctc tcgaaacaat ctggtaagaa
    26041 acaccttgaa gtatctctcc ttcaccctgc aactgctgag tttcaatggt tttaaatgaa
    26101 tctttatagc ctagcaagtt agggatactt tttgcagaca aatcaatatt ggtctgcata
    26161 ttgtcactca aagcttttag aatctcttga taatgaccaa tgctatttaa actgagagct
    26221 ttttccatga ctttttgaat aacttcacgt tgacgttttt gacgaccata atccccctca
    26281 ggatcttggt aacgcattcg tgcatagact agggcttctt ctcccccaat atgttgctcc
    26341 ccaacaccga tagaaatggt attaaattct tcttggtcac tgatagaaat tgggaaacct
    26401 aggatattat tgactgtaat acctcctact gcatccacta gtttttgcaa ccctctcata
    26461 ttgaccatca catagcgatc aatatggata ttcatcattt tttgaatggt ttctatagca
    26521 agctctgctc caccatctgc atatgctgag ttcagtttcg cttcatgagc ctgaccattc
    26581 cctgattcaa tgcgcgtcag aatatcccgc tctaaactca tcattgttgt ttttttcgtt
    26641 ttaggattca ctgtcatcaa gatcatgcta tcacttctac cgacccaagt ttcagttcgt
    26701 tcaacatttc cggtgtccac tcccattaac agaatggtta gaggttcagt cgcttcaata
    26761 accttggttt cttcgccgat ttttttatag gttttagcta aggtttctgt cccttgttga
    26821 taaatagtat aagcaacaac acctactcct actacagtta cagaaagtaa agctagcacc
    26881 attccaataa tttttttaac catatttcta ctaacctatc agtttaccca tcaagtaaac
    26941 atcgataaat ttcccttctt ctatatatgc cccacgctct tggctacctt caatgacaaa
    27001 gccatgcttt tgataaagat ggactgctgc ttgattacga gtttggacag tcagttggag
    27061 acgacgcaga atgccacttg cttgtgccca ctctatcgct tcttctagca acaaacttcc
    27121 caagccatta ttccaatatc tttttccaat cacaatgaag agatctccaa tatgacggac
    27181 tctcttacgc tgatcagctg taatatttac aataccagca attttgccat ttaagaatgc
    27241 aagtaaggtt atctgattgt ccgaactagc ttgcttgttg aggaatattt ccatctcctc
    27301 actagtcaag agaataccat ctccgtctag gctggtaaag tctgtctcca aactcacacg
    27361 atttaaaaag gccactaatt cagctgcatc tttgggctct gcttccctaa tgagcaattc
    27421 atactccata ttgaagctcc tctaacaatt tctcagcacg caaacccttt gcctgaaaat
    27481 ttaaacggcg tccatctgct tcttttagaa tttccaattc taaataagca tctggcaagg
    27541 catctcctaa gagatttccc cactcaataa cagtcacgcc accaccaaag ataaactcat
    27601 ccaagtcgat agaatcagca tctccttcaa tacgataaac atctaggtga taaagtggaa
    27661 gtcgaccttc atactctctc acgatagtat aggtgggact tttaatcatt tgagaaatct
    27721 gtaatccttt tgcaagtcct ttagtaaagg tcgttttacc tgcacccagt tctccagtta
    27781 agattaaaac atcattcttt gctaatagat ggcccaaacg ctcccctaag gcttgcaact
    27841 cttcttcatt ttttgtgtac atactcttat tataccaaaa acttttcttt tgtgtctatt
    27901 ttcctactaa acttatcatc ataacatcca taaaaaacag gctttctcta aaagaaaatg
    27961 agcgtaacaa tgaccaatac aagatctcgg aaaatatgac cataaaagga aacttccttc
    28021 ttaaccgaat ttgggacaag ataggctgca aaaaacaagc ccagtccaat ataaatcaga
    28081 agtgagacaa tggtcattgg atttcttaag aaaagaagtg ttgctaaaat agtcaccaac
    28141 actgtctttt ttctgtctag catagcaaga aaatcgcgca cgtatttttt caagggtaaa
    28201 aaaatcagca aatctagccc aaataggaaa aagaaggatg gcaataaaaa gtcaactaat
    28261 tcttgctgca gcgtattttt gatgaacaag ttatctgaca aaacaagaac agctcctaac
    28321 aaattaatta agagtaacat actgtaaaaa agcttcaccg acttcttact ggctaggaca
    28381 ctatggactt cttgcttacg ggtataaaga taatttactc cagcacagat tcctgaaacg
    28441 aaaaccatgc ttccgatgaa aaaagctgta ctttgtttaa aggacaagat gcattccttc
    28501 cataggaaac agctactcaa actgatttga attaaagcta acaaaaataa gattctcatt
    28561 gatttcatct tctctctccc ttcctaccaa tcattatact aggagaaaag agagaactgt
    28621 ttctaatctt ctcaaatgtc tctttaagac gctaaacaaa cactagagac taatactcaa
    28681 tgaaaatcaa agatcaaact aggtagctag ccacaggttg ctcaaaacac tgttttgaga
    28741 ttgcagatag agctgacgtg atttgaagag attttcgaag aatataaatt tgaaatcatg
    28801 aaaatccgtc aaacgggtgg ttgttttgtc tcgcacctca cggagcgaga cggactcaga
    28861 gtcacataat tataaggctg atagtattaa tctaactatc agccttacag gttatttaac
    28921 gtttcagaaa aactataatg tcaagattaa ctaaacagta tctagttcct tcaaataatt
    28981 ttctatcttc atcagcatta aaggattgtt ataaatctta cataactctc ttgcttctat
    29041 ataataattt ttgacttgtt ctctgtctag aaatttggct ccagcatttc ctacaagaat
    29101 aagtagagga gccaattggt agcttgtctg tctttgttta cagagttcaa tcgtttcaag
    29161 agcttcttgg atggcttcat tatatttttc ctttgatact aggtagtgag cgtagttgta
    29221 acgaactctg atgtagccaa ataaaaactc ttgatggtcc aaattttttg tctgatacaa
    29281 ctctattaaa tgagagtagt ttgcctcata ttcttgttca cgacccacta aggaatagaa
    29341 attagataga gtattcaagg cctttaaata aatcagagta tttgaagaga cttttaataa
    29401 tatattttcc aatgaagaaa ttgcctcaca cttactgtca tattgataga agtcaataat
    29461 agctttaatc cattcaaggt aagttcggtc ttctagtgtt agaaaagtac ttcgttcaat
    29521 ctctattcta taaatatatt ccaaatcgtc ataatttcta tcgtctaata gtcgagcaga
    29581 caattgcttg aaattagaga ggttagattt aatttcaatt tgttcattaa aaaaataatc
    29641 taatggtact tcaagtcgtt gagagagttt gaacaaaagg tctgcggagg gaatgaaatg
    29701 ccctctctca attttactaa tctggctttg ttcacaaatt ccttctgcaa gagtttgttg
    29761 ggagagtctc aactcttttc ttttcaatct gaatttctct gcgagtgtat tcattaaaga
    29821 taataaacct tcctatttgc ttaatttcat tataaagttt ttagttcaaa a
//
LOCUS       DAVKJD010000016        28599 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKJD010000016 DAVKJD010000000
VERSION     DAVKJD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.016"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(387..2114)
                     /gene="ezrA"
                     /locus_tag="VW157_000792"
     CDS             complement(387..2114)
                     /gene="ezrA"
                     /locus_tag="VW157_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9552123.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIDEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2196..4142)
                     /gene="gyrB"
                     /locus_tag="VW157_000793"
     CDS             complement(2196..4142)
                     /gene="gyrB"
                     /locus_tag="VW157_000793"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9552124.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFTPDPEIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4157..4720)
                     /locus_tag="VW157_000794"
     CDS             complement(4157..4720)
                     /locus_tag="VW157_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9552125.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4805..5359)
                     /locus_tag="VW157_000795"
     CDS             complement(4805..5359)
                     /locus_tag="VW157_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9552126.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5370..6593)
                     /locus_tag="VW157_000796"
     CDS             complement(5370..6593)
                     /locus_tag="VW157_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9552127.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6630..9080)
                     /locus_tag="VW157_000797"
     CDS             complement(6630..9080)
                     /locus_tag="VW157_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9552128.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPFSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVNHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9280..10209)
                     /locus_tag="VW157_000798"
     CDS             complement(9280..10209)
                     /locus_tag="VW157_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9552129.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10349..10636
                     /locus_tag="VW157_000799"
     CDS             10349..10636
                     /locus_tag="VW157_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9552130.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10670..12004)
                     /gene="ciaH"
                     /locus_tag="VW157_000800"
     CDS             complement(10670..12004)
                     /gene="ciaH"
                     /locus_tag="VW157_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9552131.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11994..12668)
                     /gene="ciaR"
                     /locus_tag="VW157_000801"
     CDS             complement(11994..12668)
                     /gene="ciaR"
                     /locus_tag="VW157_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9552132.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12777..15323)
                     /locus_tag="VW157_000802"
     CDS             complement(12777..15323)
                     /locus_tag="VW157_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9552133.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15446..15886)
                     /locus_tag="VW157_000803"
     CDS             complement(15446..15886)
                     /locus_tag="VW157_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9552134.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15861..16730)
                     /locus_tag="VW157_000804"
     CDS             complement(15861..16730)
                     /locus_tag="VW157_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9552135.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16772..17500)
                     /locus_tag="VW157_000805"
     CDS             complement(16772..17500)
                     /locus_tag="VW157_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9552136.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEAEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17508..17987)
                     /locus_tag="VW157_000806"
     CDS             complement(17508..17987)
                     /locus_tag="VW157_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9552137.1"
                     /translation="MEIKNHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVIALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGLPELDKTSYMNWKVK"
     gene            complement(17998..18696)
                     /locus_tag="VW157_000807"
     CDS             complement(17998..18696)
                     /locus_tag="VW157_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9552138.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDAYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEARI
                     QVFGIAPGAVKTAMTAADFETGGLADWVASETPIKRWIEPEEVAEVSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18689..18955)
                     /locus_tag="VW157_000808"
     CDS             complement(18689..18955)
                     /locus_tag="VW157_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9552139.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(19021..19947)
                     /locus_tag="VW157_000809"
     CDS             complement(19021..19947)
                     /locus_tag="VW157_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9552140.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(20022..20837)
                     /locus_tag="VW157_000810"
     CDS             complement(20022..20837)
                     /locus_tag="VW157_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552141.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20841..21161)
                     /locus_tag="VW157_000811"
     CDS             complement(20841..21161)
                     /locus_tag="VW157_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9552142.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(21458..21538)
                     /locus_tag="VW157_000812"
                     /pseudo
     CDS             complement(21458..21538)
                     /locus_tag="VW157_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(21582..23579)
                     /gene="metG"
                     /locus_tag="VW157_000813"
     CDS             complement(21582..23579)
                     /gene="metG"
                     /locus_tag="VW157_000813"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9552143.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(23703..24962)
                     /locus_tag="VW157_000814"
     CDS             complement(23703..24962)
                     /locus_tag="VW157_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552144.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPIALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24964..25665)
                     /locus_tag="VW157_000815"
     CDS             complement(24964..25665)
                     /locus_tag="VW157_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552145.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(25649..26848)
                     /locus_tag="VW157_000816"
     CDS             complement(25649..26848)
                     /locus_tag="VW157_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9552146.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            27113..28459
                     /gene="gor"
                     /locus_tag="VW157_000817"
     CDS             27113..28459
                     /gene="gor"
                     /locus_tag="VW157_000817"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9552147.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
BASE COUNT         8563 a         6292 c         5105 g         8639 t
ORIGIN      
        1 aaaagtgcaa ataagaaatc tccagattag gaactatccg tgagttctct agtctggaga
       61 tttttcaata gacttcgtta ttggacggtt accaagttac tcaaagtaca gatttgaagc
      121 tgcagataag actgacgaaa tcactttaat actaaatgaa aatcaaaaag caaactaaga
      181 agctagcctc aggctgtact tgagtacggt aaggcgacgc tgacgtgatt tgaagagatt
      241 ttcgaagagt ataaaacact gttttgaggt ggtggataaa tcggctgggt aaaaactcaa
      301 tctcaggaga aaatgaagta aatctcccca caactataga aaaagatttg attctgctcc
      361 tcacacaata aaatcttttt cttttattaa aaacgaatcg tttcacgtgt tttctcatat
      421 gaggtaacaa agcgattggt tacaccaggc tctgccactt ccaatgcttg agaaatcttg
      481 tcaaatgaag cgtgataatc aaattctttt tcaaaaatat ctaaagcttc gttaaatgct
      541 tcttgaatgc gttcatcaaa tgagcgatag cggttagaat attgcaagag ttgctctgtc
      601 aaagttgcat attgtacaat attataagtt tccgtttcta aagcttccat atcattcgtt
      661 gcaatttcaa gaactcgggt aacagattca atgttaatca ttttttgttc taactcaacc
      721 attaaatcct cggtattatt gcttgccgta aagaataact tcaagaaagt ttgtggaata
      781 cctggcagat tgcgtttttc catgtatcgc ttgatagtat ggagacgatt gacataaaca
      841 ttggcctttt gacgtgcatt aatatcatct ttctcaattt gtgtcaggcg ctcactaact
      901 gaaatttgct catcttcaat atcttttagt tgagtttgta aatcctcaag attttcttca
      961 agaactgaat aagcttgggt tggttcttct tgatttgaag ttacctcaac aatagctgcc
     1021 tcaaaactct ctaattctgt ctgaatacga cgaacatggc ttgcagctgt ctcaggaagt
     1081 aaataggtct tgttcaaacg tgcaatatct tctcccaata aagtattatt ctctttcata
     1141 tgttgaaggt aagttggaag agttgcaagt agattttcca ctactttctg agcagcaatt
     1201 tctcgagtaa aaatatcata caaggcattg atttcctctt gtgcctgtcc attctcatat
     1261 tcggcattat ccaattccaa ctgacgaata ttctcttggt ttttcttgaa tgcttcataa
     1321 agcaagtgga aacgcgcttc aatatccgtt tcaacaaaat gataattagc atcaattagt
     1381 ttacgataac cggattccaa atcctgtaat tgatctggca attctgtaga aagcgtcgta
     1441 accaaggctg gaacacgatc cacaatatga cttaaggcca aaatgtgatt ttctgtatta
     1501 tccaaaatca ctgcggcttc cacagggtca cccgatgaat tcaaggttac aaattgtgaa
     1561 aattcagatt ggatattttc taattgtttt tcaatttcat ccaaggcttg accatactgt
     1621 tctgaatttt cagcaactct atgctgaagt tcctcaaata aatccaaagc atgaagaaca
     1681 cgaccactat ttttagattc ttgcttctct aagtctgcca aagcattgcg aattgccgca
     1741 atatcttcgt caatcaaagt aatttgactc tcaatttggt caatttgatg actggccttg
     1801 agaaaacgaa atgaatggtt atagccttct gcttcaaaga gattattttc aatatcggca
     1861 aaagagttga gagataaatc gacccatttt tgattccatt cacggaaagc cacttgactt
     1921 tgtccaatca agtgcatatt ttttacagct tctacttcat catttactgg aagattgtat
     1981 agttcttctt ttctttcttc tagcgcctct aatctcccct cgtttcgctt acgtagaaag
     2041 attgccacta cataagccag aactaaaatg actgcaattg caaccattaa ataaattagt
     2101 tgtccattag acatatcaaa ctcctttttt acttgaaaca atcgtaacga ttatatcata
     2161 ttttttagac caagggaact acttctccct attttttaga catcaagtgt actatagaca
     2221 gcattttctt cgataaactc acgacgaggc tctactcgat cccccatcaa catatcaaag
     2281 attttatctg cttctgcagc atcatctaca gaaactctag ccatcaagcg atgttcggga
     2341 tccatggttg tttcccacag ctgatgatcg tccatttcac ctagcccctt ataacgctga
     2401 atagtcggtt tggtacgacc ttcactataa cgggctaaag cttcttggag tttgatttct
     2461 tgatctgcac ccggctggat atattcttta atctcgcttc caaccttgac accatagatt
     2521 ggtggttggg caatataaac ataaccagct tctaggattg gtttcatata acgataaatc
     2581 aaggttaaaa gaagggtacg aatgtgggct ccatcgacat cggcatcggt catcaaaacg
     2641 agtttttggt aacgggcttt cgaaacatca aattctgcgc caaatcctgt tcccatggct
     2701 gtgaaaagac tacgaatttc ttcgttagct agaatcttat ccatacttgc tttttcaacg
     2761 ttcaaaatct taccgcgaat tggaaggata gcctgaaact cacggttacg accagatttg
     2821 gctgatccac cagctgagtc tccttcgacg atgaagagtt ctgtttcagc agggttatta
     2881 gaagaacagt ctgctagttt ccctggaagg ttggaaattt ccaaaccaga ttttttacgt
     2941 gtgacttcac gcgcacgctt ggcagccaca cgagccttgg cagctaaaat ccctttttcc
     3001 acgatacgtt tggcaatctg tggattttcc atgaggaaat cggagaaggc ttcactgaag
     3061 aggcgattgg taatcttgac cacttcgcta tttcccaatt tggtcttggt ttgtccttca
     3121 aactgtggat ttgggtgttt aactgagata actgcagtta atccttcgcg aacatcttcc
     3181 cctgttaaat tatcttcatt gtctttcagt aacttattct tacgagcata atcattgata
     3241 acacgagtca gtgccgtacg gaaaccttgt tcatgcgttc caccttcatg ggtatgaata
     3301 ttattggcga aactcatgac attttcatgg taacctgttg tgtactgcat ggctacctca
     3361 actgtgatat catccatctc accgtctgta tagattggtg tatcaaagat tacatccttg
     3421 ttctcgttga tatattcaac gtaactagca atcccacctt cataatgata atgcttggtt
     3481 tgttccaaac cttggcgctt atctgtaatt gaaatttgaa gaccgcgatt tagaaaggcc
     3541 aactcttgaa tccgtttatt taatttatca aaatcaaaga ttgttgtttc agtgaaaatt
     3601 tctgggtccg gtgtgaagtg aaccgttgtt ccagttctat ctgtatcccc aaccacctca
     3661 aggtctgcaa caacatgacc acgacggtat tcttggtaat gaatcttacc gtttttatgg
     3721 acatgaacgt ctaattgagt ggaaagggca ttaactactg acgaccccac cccgtgaaga
     3781 ccacctgaaa ccttgtatcc accaccgccg aactttcctc cagcatgaag gactgtaaag
     3841 acggtctcaa cagcaggacg gcctgttttt tcctgaatat cgactgggat accacgccca
     3901 tcatccacaa cagtaatcga atcatctggc tcaataaaaa cttgaatatg gctggcaaat
     3961 cctgccaagg cctcgtcaat tgagttatca acaatttccc agactagatg gtgaagacct
     4021 tcttttgagg ttgatccaat gtacatccct ggacgcatac gaacagcctc taagccctct
     4081 aaaacttgaa tttgactggc atcataatcc tgtgcctgca gatttttgat ttcttctgtc
     4141 atcttattcc tttttcttat atgtctaata cttgtcttaa attcacgata ctggtaaaca
     4201 agtgggtatc aagtcctgca gcttgacctg cttcgatatc aatcggccga tcaccaatga
     4261 caagaccaga gctaatctga tacttttctc ttaaataaag catggattcg ggatttggct
     4321 ttctcttaaa gcctgagcta gaagtcacca cttctgtaaa ataagctgct atagaggttt
     4381 tttctaaaat ttccaaaacc tgatcatttc gatgagagac caaaaaatga cggccacctt
     4441 gatttaaaat gtcttccaat aggtcagaaa ctccttcaaa taaaatcggg tgttcaagct
     4501 ctctggcttc attttccttg tacttttcta aaaaattctc taaattggga gcgaatgtct
     4561 caatcgcaaa aggagtagaa acctttaaag cttgatagac actgtcatgg tcttgtgtga
     4621 taccatacag tgccaatgtt tcaacaaatg cagctgttga agtttcataa ttatccagta
     4681 aagttccacc taaatcccag atgtaatcgt gatatttcat acccttcatt atagcatttt
     4741 tttatgaaaa aagcgatgat ttccctgagt tttccacata aaaaacgaga gaataactcc
     4801 cgttttactg atttttacca aagacatctc gatagagcat tcctgttcgt aaactctctg
     4861 cgtcccctcc tagttgctcc accaactcgt aggcaaaggc aagggctgtt gaaggacccc
     4921 gactggttgt caactgacca tctaccacta ctgtttcctt gacgtagtga ccatcaagga
     4981 tttgctcttg aacgccgtca taacaagtgt atcgcttatt tttcaatatc tctgcttgat
     5041 tgagggcaat tggtgccgca caaatggctg ctagtttctt cccttcttgc tcgaagcttt
     5101 gcaattcttg aatcaaggtc tgattatcac gtaaatgtgc agaaccaggc atacctccag
     5161 gaagaacaat catatcatag tctgataaat ctccatcaaa gacatgatct gctcttactt
     5221 ggattgcatg cgaacccgtt acttgctctt caaaaccaac catatcacat gtgatattgg
     5281 ctcgacgcaa gacatctaca actgtcaagg cttcaatttc ttcaaagccc tgagctaaca
     5341 taactgctac ttttaccatg attttctcct tatttaattt gttttaatac aacctttttc
     5401 cgtttgaatg ggacttttcc gctcttttct tcagctaaat tagtctggta actcatcgat
     5461 aaaagcaaac caacaccaat caaattactg ataatagctg atcccccttg cgaaatgaaa
     5521 ggcaagggaa tccccgtcaa aggaagtagt ccagtcacag caccgatatt ctcaaagatg
     5581 tggaagagca acatcataat caaacctgtg gaaatataag tgtagaactg gttatttgat
     5641 ttaagagtaa tcttcaacat acggtaaatc aacatgagat agagggcaat aaccaggaca
     5701 gagccaataa agccaaaatc ttctgcaata accgtaaaaa tcatatctga ctctcgaact
     5761 gggataagca gattcgaagc attaaatccc tgaccaaata agccaccact cccaatggca
     5821 atctgccctt gagcctgctg gtaagtcgtt gtttgggcaa actcaaaggg attgagccaa
     5881 gccaaaatcc gattaatttg gtaggtcggc attccaatct ggtgaagaaa agctcgtccg
     5941 tccttgctaa taaagatagc taagaaacca gcaactcctg ttacagcagt cacaaatact
     6001 gggataataa ttttccaaga aacccctgat aataaaacga ttcctgagaa aatggctaca
     6061 aaaaccaaag ccgtccccaa gtcactttga agtgctaaaa gaactaggac tggaatggta
     6121 aagagaatca tccagaaaat taacaaaaag tccagcggaa ccgtgcgtct ccattcctta
     6181 tgtttctttg taaattggac aatgacacga gccaacatga ggatatagga tatcttcata
     6241 aattctgacg gttgaaatag ggtaattcca tttattgata cccagttttt ggcacccgtt
     6301 gatgcaacta agcttggatt ataaaataca atcggcaaga tcataagtcc caagcctaaa
     6361 atatatagaa agggggtcac cttccaaaga aattctgtat taaagagcat gaccacaaaa
     6421 ccaatcacaa gccccaaggc aatccaggcg acctgctgcc ctaaaatggg cagaatatta
     6481 ttgggataat catgactaac ggctatatag atagccacca caccgatgac cagtagaaaa
     6541 aatactggca agagcaaact ataatcgact ctagagtcga gagaacgttt catatgtact
     6601 aaccttatac tttcatacaa tactatttat caaagttcat taaaaaatct atcaatagcc
     6661 tcgtcaactt cggatcgaga gatggtttta acagtcgctt cttctgctag agatgctact
     6721 atttgtttgc cgtatcgttt tccgacgatt ctcctatcca aaataagagt taaggaacgt
     6781 tggtattcac gtctcatact tcttcccaaa gcctgtttta aacgaataat ggccattggc
     6841 aattgataat cataaaaggc atttttccct tcttgattca gttcttgatt aatctttttc
     6901 gtcaagggtt cttgaggatt ttggaaagga agcctcggta caacttgaat cacagaagga
     6961 tggcttgaaa aatcaactcc ctcccagaaa cttgctgcac caagcaagat ttgttgttca
     7021 cctttttcaa agcgtttctt tagctgatga acatccccat ttttatactg ggccaagtgg
     7081 ttaactgtaa gtaaatccga tactgctaga agcatgtctt tagcggtaaa gagaaccaaa
     7141 atcggttgct ggaaagcttg aatttccact agtaaagcag ctacctctct ggcatagact
     7201 tctaaggagg tttctgttac cagagggaaa tctttgacca taaccacttc ttgttcctgt
     7261 tttccccgag attcaatctt gacaaatttg gcttcaggat agcctaaaag gtctgccaaa
     7321 gaaaccctct gactaatctc aagagtagcc gatactccca agacttggca actctcagga
     7381 agtaaagagg acagaagagt acggcctgat tttgtagaag aaatctgaat tttcttttga
     7441 ctcgtttcag ttacttcaag ccagtaatca ccttcagctg taaaatagcg aaccagctca
     7501 tcaaagcctt ccacttctaa ttctgaaaaa tactgatgga gattgtccag agaatctaag
     7561 atatttttcc tagatttacc agactgaaat tgttctatca agtagagaca ctcaaagcga
     7621 atactttcta gtatccgttg ttgaaccctg ttttcttctc ctactaaagc cttatcaatt
     7681 aaatcgataa tagattgtat atcgtaggtc tcttgaagca gattttctag agctaacaaa
     7741 atcttttgga cttcatcaat aatcagtaaa cggtcactga caaattcagg attatcttca
     7801 agtctggtca cgagataggc atgattagtc actaaaagct tgcaagtctc tgccctttct
     7861 tgactacgtt tccaaaaatc ttccgtcaca aataagctct gggatgataa attcccatca
     7921 tgacgaaggt ctgctagaaa atgttggtaa cggtagagtt gcccgatttc atccaaatct
     7981 cctgtctctg tctcagtaag ccagaccaag acttgcattt taaagcgtct aaataagcga
     8041 ttttcatcat tttcctgcaa ggaatgataa aaggcatcca acttcagata attttgtggt
     8101 ccctttaagc tatgaatatc tgtatggaac acttccttga gacgtttacc ttcttcttcc
     8161 atgatttgat tttgaagaat ctttgtcgga acactaagaa caatttgtcg ctctttggat
     8221 tgagataaag cgggtaagag atagccatag gttttcccaa tccctgtcgg cgcttgaatc
     8281 agagagaaag gttcatcttt caatagcaag ccaacctctt tagcaaaact ttcttgttcc
     8341 tccctcactt caaggttcaa cagagaaata tttttagaaa agtcttgaga tagttttcgt
     8401 agctccagag aagctgccgt tttcttaaaa tacagacctt gaacttggac caagtctgga
     8461 gaactcagga tagattggtt gcgataagtt tcctcaataa ccaggtaaga ctcatatagg
     8521 agagcgtcag ccatttccag caagcgttcc aagagacctt taggaagctg ggtcatcttt
     8581 tcccgtaaaa aaagaagtaa ttctgctgta gcttgggcat ctgaaagggc tgtgtgtgcg
     8641 tgtttaagag gaattcctaa ttctcgacac aaaatcggca agctatattt ttccagttca
     8701 gggaaaaaga cctgggccaa ttcgaccgta tcaacacgag ggtttcttag ctcatagcct
     8761 tcaaaaaata aattttccgc caagagatta gcatcaaact gaacattatg ggctacaaaa
     8821 atcccatcct ccaccaagtc aaagattttt ctggcaactt gcgaaaaatc aggtgcttgc
     8881 gccagacgtt ggtctgtcaa tcctgtcagt tctttgatat gagcatccaa gggttcatgt
     8941 ggattgacat ccgtcgtata gtgatcgacg atttctccat cctcaatcac gacaattccc
     9001 acttggataa ttttagcctt actacctgtg ctagttgcct ctaaatccac cacaacatag
     9061 cgattaccaa tcgttttcat tataaaaaat tataccaaaa aaagggccat ttggcacctg
     9121 caaacatagg ataattttca aactcaagaa cactactttt cttagaaatc ccatcgaata
     9181 gagcattcta gcctgctgat ttttttaaaa tagtgaagaa atacgaaaaa ttgagagaag
     9241 aaatgttttc atcttccttc tctcaacgaa tcatgtattt tatttaacca tgtcaggatc
     9301 gtagtcataa aatcctgtat caaggaaacg gtttttaggg tgaagtttac gaacttcttc
     9361 atccagcaag aaggcttggt catggctaaa gttgccctct tgaatcaagc tacgcgcttg
     9421 gataaagagt tttgcaatct caacaaagtt ctgaccagga gtgtagagcc cttctagttt
     9481 ccagcgagta aagccatgct ccaccaattc tgttaatttg atcatcaaat caaggtcatt
     9541 gttggcaaag atatgggtcc catgattatc ttcaaaaatg gaatagtggc tctctggatc
     9601 acttggctca gccaagaaga ggtcacgttt atgcgtcttt tcatcatcga tatgtgtaaa
     9661 gttatagtag ttttgcaaga gtggacgttt agaatgatgg atgacgctag caccgtaaac
     9721 caaaacttca gcaggaattt ccaaaatctc tggcattttg aaaagttcag ctgatggaat
     9781 ttcacgcgcc aaaacagcct cagatgcgcc agccttttgt ccccagaagt taatctgacg
     9841 actgctagtt accatggttg aagcatcgta gatggtctta aatgaataac catcgcggtt
     9901 aactacgtaa aagacgcctg catccccaat cgtaatatag tctgtcttga tttcttccaa
     9961 gaagtctaag aaaggcttga tacggtccat catatcttgg tgcatgagag cattgaccgc
    10021 aacgatcaat tccttaccag catcatgaac caacttagcg atttcacgta attggtcata
    10081 actaaaggtc gttggcagac gaaggccaaa atctttctca ccgacataga tacggtctac
    10141 gccagcttcg agtagttgtt caacttgttc aatactttca gcagttgctg taatgacaat
    10201 cttttccata agaaaaatta taccacattt ctccaaaatc agccatgctt tgaaaatgaa
    10261 aagggacttt attctttttg taacctttct gtaataattc tctgccgaaa aaatgataaa
    10321 ataggtatgt actgttaagg agagaatcat gcccgtaaga aaattacaat cctatgaggt
    10381 agactatcaa gaagaattaa accagcagct tcctcattac caagcttata cacctgaagc
    10441 gcaatctgat gccaatctca aggaaatcct attttttatt aatatcgctg ttttttgtat
    10501 ctgtattgct atctttagtt ttatcttttt agcattaaaa ttatcaactg ctcttgcctt
    10561 tgccgcagca atcggattca gcttacttgt tttaaaagtt caacggtcta ttatcaaacg
    10621 aaaacgtaga agataaatcc aaaatatcgc cccaattgga gcgatatttt tattttttct
    10681 ttttagatgg tgtctgaatg gcaatcttca cttcaaagat tgttcccttg ggtttattat
    10741 ctttgacagt aacagttcct tttagagcat ctacaatttg cttggctagg gataatccta
    10801 aaccaaaacc acctttttgc cgggttctag ctttgtctac tcgataaaaa cggtcaaaaa
    10861 ttttcttttt atcttctgtc gaaataccga ttccattatc agaaacaagt aaataaagat
    10921 tgcgatcggt cgccgagata agaaaatcaa tttcaccatc ctcctcagta tacttgacgg
    10981 cattatcgaa aagaatggtc atcagttgtt tcagaagaag ctgatctgtg acaattgttc
    11041 gatggatacg attttcaaaa cggaagacac gattattttc cgaagcaatc atctcatagt
    11101 ttgtgaaagt tgtattaaaa aagctagttg gaacttctgc aagctccggc ttaatcccat
    11161 catctctccg agctaagttc agcaagcttg tcgttaaaaa acgcatattt cggacttctt
    11221 ccaaactcga tgcaatgctt tcgctcacat ccataatggt agcttctggc ttacgaaaaa
    11281 gggtctctaa gcgattttgc aaaactgcga gtggagttcg taactcatga ctggcatttt
    11341 ccacaaaaga ctgttgcttc tgcatactct caagcagggg cctaacactg accctagcta
    11401 gatagagact ggcaagtaaa gacaaaatcc agaaactagc catcacgacc acaatcaatt
    11461 gctcatgctt ttgactagcc tgctccaact gactggtatt aatcaagaca gcagcatact
    11521 tgatattggt tgaaaccgaa ctaatattgg tctccatcaa aatcacacga tagatttctt
    11581 cctgcccata gctattaaaa acctgaatct ggtagatatg tcctagttct ttcttttcta
    11641 acttaatctt atccaagccc aaaaatcgat ttccagaaag aagttgagta aagtctttat
    11701 caaagagaat gacttccgta ttggaactga cattaggttt tatttctact ttactagcgt
    11761 ccgccctagc attttctaaa tctttaatct cttctgttgc cctatttata gccagctgaa
    11821 taactgcttg aggattttca ctcagtccat gaagcttatc gtccaccgaa gtataaagac
    11881 tcgaatgcat gacttgtaaa ataatcagag tcattgtaga aaaaatcagg gtgaagacac
    11941 cgaagttgcg gataaaataa ctaaagtcat ccgcatacca tgttttttta agtttactga
    12001 acatctttta aaagataccc aacactacgc aaggtttgca aattctctgc aaaagtggtt
    12061 ccctttaatt tcttacggac ttttgaaaca tagacttcga caaccgaaat cgttgtatca
    12121 ctatcaaatc cccatagacg gtcaaaaatc tgcgtcttag gcaaaatcac attttgattt
    12181 tgaaggaaat aaactagtaa atcgaactct ttccccagca attcgacagg agtatcttca
    12241 actttaacgg tattggttga taaattaacc acgatattcc cataagtcaa ggtgttttca
    12301 ttaaacttcc ctgaacgttt gagaagggcc tgaattcgca ttttaagttc ttctaggtag
    12361 aaaggtttgg tcagataatc atccgctccc agttcaaatc catgtccctt gtcatccaaa
    12421 ctttccttgg cagtcataat cagaactggt gtcgtaattc ccttttcacg caattctttt
    12481 aagacttgga aaccattttt ttctggcaac atcaaatcca gcaaaatcaa gtcatagaca
    12541 ccactctcag cttcgtagag accttcttct ccatcaaata cctgcataac atccgcaaaa
    12601 tcgtctaaaa agtcaaatac tgaatttgac agacctaggt catcctcaac caataagatt
    12661 tttatcatga gaaactcctc cttattaaaa ctattatacc aaatttgcct taaaaaaaac
    12721 tcaactctct gcattttaca tgagatagct gagttttctt tttattttag gcttatttat
    12781 gcatttccgt attgaagaac aactgcttcg actgcagctt tttcacggct aatcaagtca
    12841 acacgtgctg caatttcctt gattcccata ccgatgttac ggctaagagc aaggtcagaa
    12901 agttgcggct caaagaactc cttgtattcc gccaagcgtt gctgagtctt aaatacatga
    12961 gcaggaagga taacaaagct atcaaagctc atatctcctc caagggctgc cttaatccaa
    13021 gcccagtttt cacgcgccca agaccaagct gttttctgag ttgcttgatg agctaggaat
    13081 tggtaatacc aagcagacaa gtcctgtggt ttgaccacaa atttgtcctt ccaagaagta
    13141 atcaagtttt ggatattatc cgcatctgta ctgtatgcaa gagctgctgc caactggcgt
    13201 ttaaagacag catctgttgc gtgagtataa gtatcaagat aaagtgctaa caagtcttta
    13261 gtctcatgat gtttcatctc attaatcaga acttgtgaac gaatagctgc tgggagttct
    13321 gcaagattct ccttgtgtgt tgcgaagatt tggctagcga cttgacgagc ttctgcatca
    13381 tttgagcgaa tcatcatcga aacagccagc tgacgaacca attcatcctc atctgattct
    13441 ccgtctttag cttcaaaacc aagacggtca tagttatgac gagccaattt agcaaccagt
    13501 cctttgaagg ctgtttcagc atccgttcct tcatcaataa agcgctcaag ggctgaaatc
    13561 acttgagaaa cagctgaaac caccagataa gactcttcct tagcaagttt atcaagaact
    13621 ggaagcaagt ctgcataaga aatgtgccct gcctcagcca acaaacgacg ttcttgaaca
    13681 atttgcagtt tgcttgtgtt atcaagtgtc tctagctcag caagaacagc ttctaacaag
    13741 tctccttgat agtcggtaat atagtgggca gtattttcag tgttgagacg aagagctcct
    13801 tcattttcag caagaagagc tgcgtagcca gggatttcga tactttcagt ttcgagtgta
    13861 tcaggcaagc ctttccagtt gctattgagg ggcaccaccc agagacggtt cttgtcttcg
    13921 ttctcaccga tgaagaattg tttttgtgaa atcttcaaga catcattttc aactttgaca
    13981 gtaagaactg ggtaaccagg ctgttccaac caagaatcca tgaaggctgc gacatcacgt
    14041 cctgacgctt gaccaagggc atcccaaagg tcactaccaa tggtgttgct gtattggtgt
    14101 ttttcaaagt aggcatgcaa acctttagca aaatcagcat ctcctagcca acggcgaagc
    14161 atgtgcatga gacggcttcc tttggcatag acgatagcgc cgtcaaagag tgtattgatt
    14221 tcatctggat gtttaacttc gacgtggaca gactgaacgc catcagtagc gtcacgttca
    14281 agagcaagag gtactccacc tgtttggaaa tcttcaaaga tattccagct tggttcgatg
    14341 gtatccacac agacgtattc catcatatta gcgaaacttt cattgagcca aaggtcatcc
    14401 caccatttca tagtcacgag gttcccaaac cattggtgag ccaattcatg ggccacaaca
    14461 agggcaactt gttgacggct agcaaatgta gagttctcat cgacaaccaa gtaaacttca
    14521 cggtaggtca caagacccca gttttccata gcaccagctg agaagtcagg aagggcgatg
    14581 tggagagatt gaggaattgg atacttaact ccatagtaat cttcgtaaaa ctcgatagag
    14641 cgaacagcga tatccagtga gaaatcaaga tttgaaagtg gatgtgcttt ggttgagtag
    14701 acacctacca gggtaccatt tttagtttta gcggtcaccc cttgcaaatc accagcaaca
    14761 aaggccaaca agtaagaaga catgcgaggt gttgtctcaa acttccagat acctgtttcc
    14821 ttacggtttt caacatcgat ttctggcatg tttgacaagg ccaattcacc ttctgcttgg
    14881 tcaaagcgaa gagagaggtc aaaagttgct ttggcttcag gctcatccac acatgggaaa
    14941 gcttcgcgcg caaaatggct ctcgaactga gtagacaaga cctccttctt gactccatca
    15001 actgtataat aagaagggta aatccctgtc atgttgtctg taattttacc agaaaaggca
    15061 agaaccaatt caacttgacc agcctcagcc aattcgatat gaagggcttc attgtcatgg
    15121 tcaactgtaa atggacgagc ttgacctgca acttctacag aggtgatttc caagtctttt
    15181 tggtggaggg agatgcggtc actctgtgct tgaccagtga tggtcacttt cccagaaaaa
    15241 gtcttggtct cacgactcaa atctaaaaat aaatcataat gttcaggaac aaattgctta
    15301 ataaaatgtt caactgcttg catagttttc tcctattcta agtttaagag ctgctgctct
    15361 tcttcaaaca aacttattat atcatatttt gcttgaaaaa aaaggattgg acagcgattt
    15421 ccaaaacttt cttccttcta atagtctaca gtgggtagac cttgcgaaat tcttctaaaa
    15481 caaccttgct ttcaggtgta aaagtcagcc ctacttcacc caaacagtct gtgaaaaagt
    15541 cctcatgaac ctggcgccaa taggcaagtg atttgtctcc ctcaccttcc ttaaaggcat
    15601 gttgcgcaga aacttgattg aagagttcaa cagaaacctt tgtaatttcg acaatgcaga
    15661 cagactgatt ttgactatct aaaatgatat cgaaggtccc tacttgggga agtggttcgt
    15721 cttctagtac atagaggtca taggctgatg ctgttgctgt cttttctcct ttaaacacca
    15781 aatccgctaa aagatctggt tcaactccaa aagcccaagc atcgatttca tctccgatca
    15841 aaggattgat ttgcttgtat ttattccaca tttcttgcgg tatcatgggt actcctttgt
    15901 aattttttac tttcttcttt tatgtgttta agatgatctg gatggtcaat ctctaaatca
    15961 aaaatctctg gaatagaact gtagtggata atgcacttga tacccaactg attcattttt
    16021 tgtatgaaag aagtattcag atagcctgct acagcaaaat caatcttgtt ctttcttgct
    16081 ttatcctgca tatctcttag catatctaac attattggac tttccatatc atgccattga
    16141 ctgtttctca tagtcgcaaa aacaaaggaa gtcaaatcat tcattccaac tacaatcttt
    16201 gaaatgcccg tttccagtat actagataag tcaaaatacg ctgacggtaa ttcaatcatc
    16261 gttccgactt tcccagtaaa accctgctga cgcaatactg taatagcttg ttttaattgg
    16321 tcggcatcat tgacaaaagg aaagataaca gatagattgg ggttggtttg ataaacttct
    16381 gtaacgacat gtgcttcagc ctgaaattca tccaaacacg ccagtaaacg cctggttcct
    16441 ctatagccaa acaagggatg cccttcgtca aaaaactctc tagtccccac taaacaattg
    16501 gcttctgtat tcgttaattc agaaaaacga taccaaactt cttcatctga gtacagggag
    16561 cagatagtct ctagataatc ttttacaaat tgctgacaac ttgctaatat gatgttttga
    16621 ttaagctctc tcaacaaata ttccccacga atcatgccga cgtggtgaaa tagttgagga
    16681 taaatttttt caagaatttt ttcgccacta agggcaagtt gatttctcat agccattcac
    16741 ctcgtcttaa aagatacaat atcatatata attatatcaa aatttcctct agttcaaaag
    16801 cttcataagg ttcagataac tcatatccta aaaattctgc tacttttttg gaagcctcat
    16861 tatgagcatc ccaaagtgga aacatatctc tatttaaact ctctagaatc attgcagcac
    16921 caagcttttt agcaaatcca tttccttgtt cgtttggtct agttgcaact tccgcttcaa
    16981 ctgctttacg gtaaactaac cctgaggaaa tcccagcaat cagttcatta tttttaagaa
    17041 tcacaaagcc aaatccacct tttaaaacaa aatcctgata ggactcaaaa tccccctgta
    17101 aatcctgtga ccattcttcc gtagaaaagc agttataaat atgattatca ataggcacaa
    17161 tattgtaacc ttcctctaaa tgagtaacta gattatttaa aaattcaact tgaaaatttg
    17221 ccttatcttt aaaagaatag cgagtaaaag aatttagccc cacttttcga tgcagaaaat
    17281 cttgccaatt agtattttct gaaataataa tcttataatc taaaccatat tttctaacaa
    17341 aatcttccca aaatctggaa tccaactccc ctgctaggta tagaaaattc ccaacatcat
    17401 aaaagcttgt tgtcctatca ttgtttcggt agacagttcc aatttgagat ttaagaccat
    17461 agagaaccat atttttcttc catgttttaa atagttccat tatccctcta cttcaccttc
    17521 caattcatgt aagaagtctt gtccagttct ggcaatccta ataattcaga cttaaccttc
    17581 aagactaatg gcgatgcatt ttcttctgta atctcttgaa tatccatcca aatatatcca
    17641 agtgaatcat tcgcaccatc agacacagct tccgaaatcg taacttgagg tgcactctca
    17701 ttcatttcaa catcatacaa ggctatgaca tggtgaacca taaaattttt taactcttcc
    17761 ctgacgaaaa catcgtagat tcgaggatta gagtagcttc taacagtaaa tcccgtctct
    17821 tccataactt ctctagtcag cgtttccgtc agtccttcac caagttgctg actgcctcca
    17881 ggtagatcat accgatgttg ataagggcct ctcgtttttt caatgcaaag taactttcca
    17941 ttttcaaagc aaacacagta gaccccaaag tgatttttga tttccatcca actcctccta
    18001 cttcaaagac cagccaccat ctattgtcag aatttgtccc tgcatggcag atgcctttcc
    18061 actggccaaa aagagactga cttctgccac ttcctctggt tcaatccagc gcttgattgg
    18121 ggtttcacta gccacccagt cagctaagcc acctgtctca aaatccgcag cggtcatagc
    18181 tgtcttgact gctcctggag cgataccaaa gacctgaatc ctagcttcag catagtctag
    18241 agccaactgc ttggtgaagc cagccaaggc atgcttggat gaagtatagg cgtgaccacc
    18301 tccacctgct aggctagaag caatggaaca catattgatg atgattccct ttttattttc
    18361 cagcatttgt gtcaaataat accgagtcaa ctctactgga ataatgtagt tgatttcaaa
    18421 aatctcttga atgtcctgcg ccgtttgttc caacagtggt ttgtaagcat ccaaaactcc
    18481 ggcagtatta cacaaaacat ccacctgagg gcaccagtca aaaataggtt ccaagtccaa
    18541 ggtcaaatct ctctgtaaaa agtgaaaatc accctctaag agtggctttt caccttggtc
    18601 aactccataa acttgatagc ccttctctaa aaagaggcga gcttgagcca atccgatccc
    18661 tgaactcact cctgtaatga gtacacgttt agtcatgcac ttctacccaa tccgttgcca
    18721 aaacatcaca aactgtcggg ctccacatgg aaaaaccttc tccttctcca gaaacgttga
    18781 ttaggaaata aggtgtcatt tcaagtgcaa gcccattttg ctcgatggta tcaaagagtt
    18841 ggacatagtt ttccgcacct ccccaaccag ttcgtacata ttttctctta gcctttaacc
    18901 caggcaggat ctcttcaaat gtcatgtttt tctcctttaa ttctacattc ttcatttaat
    18961 tatagcaaaa aaccacttta tacggctttt tgactgtgag ttattcaaac ctgctactac
    19021 ttacggcaaa ttattccctg cagcaagata aatttcatac cattcttttc ttgttaagct
    19081 aaagtttgcc gctcggctaa cttctctcaa gtgcttagga tttgttgtac ctacgactgc
    19141 ctgcattttt gctggataac gcaatatcca agaaatggca atagttgaag aggttactcc
    19201 atatttaata gctaaacgat caagtacttg atttaaagct tgaaatttct catttccaac
    19261 aaagttccct ttaaaatacc cgaattgcaa gacagaccat gcttgaatga ccacatcgtg
    19321 taattggcaa tattcaaaaa tgctgccatc tcgcatagct gcttgactat cttccatatt
    19381 aacatgaaaa gctgattcaa atcctggagt aaaagccgca ctcaattgta gctgattaac
    19441 agctaacggc tgcttgacat cttttttaag caactccatc atcataggat tttgattaga
    19501 aactccaaaa tctcgaactt taccttgttt ataaaggaga tcaaaggctt ctgctacttg
    19561 gtcagattcc atcaaagcat ctggtcgatg aaggagcaag ctatctagat gatcaatctt
    19621 caatctttgc aaaataccgt ctactgattt tataatatag tccttagaaa aatcaaaata
    19681 ggtaaattct tcaatgcgaa tgccacattt ggactgaatc cacatctttt ctcttaaatc
    19741 tggacgattt tttaggacaa gacctaacag ttcttcacaa cgaccacgac cataaatatc
    19801 agccaagtcg aaggcattga ttccaacaga aagtgctgtt tctacaagct cttcaacttc
    19861 ttttacagat ttatctttta ttctcatcat tccgagaaca atttctgata attctttgtc
    19921 atcttgacca agagttatgt atctcatcaa atttttctcc tttaatttct aacattcttc
    19981 ccttcattat aacaaaaaac cgctttgcaa cggctttttg actatacttc actccatttt
    20041 atcttcttaa acccacggaa caagacaaag attccaataa agaggacagc taaaggaata
    20101 acttttgtaa ggaaaacatt tgaaattccc atccactcat aataacggag cagagaaccc
    20161 accacaagat gggcaataat catactgaca aatggacgaa agaccgcttc tttccaattc
    20221 caaataccga taactagcga aatcgtaaag acagacaaac tatcccaggg agccggaata
    20281 taaaaggctc cttcttgtat gaagcttgcc attcctacat atcctaaaac aactagaaga
    20341 actatagtcc caacaacaat gtaagtgcca attttcattt taggagaatc ttggactaaa
    20401 cttcttcgta aaattgtggc cacaagtcca aatccaatca gaaaaataag aagttgccct
    20461 aaaaatgtga gcaaattgac tgttaataga ggacctttag aaaaatcact tagtagttga
    20521 taataacgta ataccgccag gacaagaatt ggcgtcaaaa gggactcttt gatagaactg
    20581 cgaggtgctc ccttgagaat ctctttcatt atttttttag gattcttacc tagataatcc
    20641 tctgcactca tgccatctcg ttctgcttct gagaaatcta gcatcatcaa atagatctgc
    20701 tctctgagat agtcttcatc atagagaaat ccagcaagat taaaactttc ccacaactcc
    20761 tcaaaatact tttgattctc ctcagaaaac tcatgtagca aagcgcttgt ttcttcgtaa
    20821 tacttcattt tcttcatggt ttaaccccca ttcttaatcc cttctacttt ttgactcaaa
    20881 tcgtcccatt gttgccaaaa gactgagaca cgctcttctc cttctttcat taatgaaaaa
    20941 tacttccgat ctggaccatc tggcgacggg cgcatgtcgc ctcttatcca ttgatttttt
    21001 tctaactttt gcaacaaagg ataaatagtt cctggaacga tagtatcaaa tccagcctct
    21061 cgcaaagtct gaaccaactc ataaccatac cgctcttttt gaccaatcat atccaagaca
    21121 caaccttcaa gaacaccttt taatagctga gtttctttca tcacttttcc cttctaatct
    21181 attttgtaat acctactagt aacttcacct atagtatatc acttctacac tagtttgtaa
    21241 agcataatag ttaatactct tcgaaaatct cttcaaacca cgtcagcgtc gccctacctt
    21301 atgtatggtt actgacttcg tcagtttcat ctacaacctc aaaactgtgt tttgagctga
    21361 cttcgtcagt ttcatctaca acctcaaagc agtgctttga gctgacttcg tcagtttcat
    21421 ctacaacctc aaaactgtgt tttgagctga cttcgtcagt ttcatctaca acctcaaaac
    21481 tgtgttttga gcaacctgcg gctagcttcc tagtttgctc tttgattttc attgagtata
    21541 aataaaaaaa cagaactagc ctgaactagt cctgtctact tttacccaat cacacttcca
    21601 tttggtacag ctggatcaac tgtgagaagg gttaatttgc catcatgttc agctgagaga
    21661 atcataccct ggctgacata ttttttcatc attttacgtg gtttgaggtt agcaatgatt
    21721 tgaactttct tgccgaccaa ttcttgttca tttggatagt attttgcaat tcctgaaaga
    21781 atctgacgat cttctccatc accagcatcc aagcggaatt gaagcaactt atctgaacct
    21841 tctactttag acacttcttt gacttctgcg acacggattt caaccttgtc aaagtcttca
    21901 aacttgattt catccttgtt tagtttgagc tcaacttcgt ccggattcca ttctttttcg
    21961 actgctggtt tattgccttc catttgttcc ttgatatagg cgatttcttc ttccatattt
    22021 agacgtggaa agataggtgt tcctttggca actacagtca catctgctgg gaagtcagcc
    22081 aaactcaagt tttcaagact agaaacttct tccaaaccaa gttgagtcaa aactgcacga
    22141 ctagtttcca tcataaatgg ttcaatcaag tgagcaacta cacgaatgct ggctgccaag
    22201 tggctcatga cacttgccaa ttggtcacga agagcttcat ccttggccaa gacccatggt
    22261 gcagtctcat cgatgtattt attggtacga gagatcagag tccagactgc ttcaagcgca
    22321 cgtggatagt caactgcttc catgtgtgta tggaagtctg cgattgattg ttctgcaacc
    22381 tcagcaagaa catgatcaaa ttcagtcaca ccttctacat aggcagggat ttgtccatca
    22441 aagtacttat taatcatgga aaccgtacgg ttaaggaggt tcccaaggtc attagccaat
    22501 tcatagttga tacggccgac atagtcttca ggagtaaagg ttccgtctga accaactgga
    22561 aggttacgca tgaggtagta acgaagtgga tctagtccat aacgctctac caacatttca
    22621 gggtaaacga cattcccttt tgacttagac atttttccgt ctttcatgac aaaccaacca
    22681 tgggcaatca aacgatcagg taatttaaca tccaacatca taagaaggat tggccagtag
    22741 atagagtgga agcgaaggat atcttttcct accatatgga agactgttcc attccagaac
    22801 ttgtcaaagt taccatgttc gtcttgagcg tagccaagag ctgtcgcata gttaagaagg
    22861 gcatcaatcc aaacgtagac aacgtgtttt ggatttgatg ggacaggcac tccccatgta
    22921 aaggttgtac gagataccgc caaatcttcc aaacctggct cgatgaagtt gcgtagcatt
    22981 tcattaaggc gaccatctgg cgtgataaat tcaggatgag ctttgaaaaa ttcgaccaaa
    23041 cggtcttggt atttgctaag gcgaaggaag tatgattctt cagaaaccca ttcaacctca
    23101 tgacctgatg gagcaatacc accagtcaca tttccagctt catcacggaa aacttctgcc
    23161 agctggcttt ctgtaaagaa ttcttcgtct gatactgaat accaaccaga gtattcaccc
    23221 aagtagatat catcttgagc aagtaagcgt tcaaagactt gtgcgacaac tttttcatgg
    23281 tagtcatcag ttgtacggat aaatttatcg tatgagatat ctagtaattg ccagagttct
    23341 ttaactccaa ccgccattcc atcaacatag gcttgaggtg taataccagc ttcttccgct
    23401 ttctgctgga ttttctgacc atgttcatca agacctgtca gataaaatac atcgtagccc
    23461 atcaggcgtt tgtaacgtgc taggacatca catgcgatag ttgtgtaggc agaaccgata
    23521 tgaagtttcc cagatggata gtaaatcggc gttgtaatat aaaaattttt ttcagacata
    23581 atttttcctt tccaggcaaa tgaaacctgt ttttctaaca cttcattata tcacattttt
    23641 aatgaatttc gatagggaaa tccatacaaa aacaagatag acgagtgtcc atcttgttga
    23701 tctcattcat aacgaagggc ttcaattgga tcaagtttcg atgccttgtt ggctggcaag
    23761 actccaaaaa tcataccaac actagccgaa actgcaagac taaatagggc aattgggatt
    23821 gatactccaa cttctatacc ttctattaaa ccttgcagta acaaacctgc taaggcagtt
    23881 aaaccacttg caattgtcaa gccaattaag ccacctaaca aggtcaaaat catggattca
    23941 atcaaaaact gaattaaaat attggcacgt gttgcgccca aagccttacg aagaccaatc
    24001 tcacgagtgc gctctgtcac cgaaaccagc atgatgttca tgacaccagt tcctccaaca
    24061 aagagagaaa tccctgcgat ggaactaata atcgtcgtca taaaactaaa cgattgttga
    24121 atttctgcaa atacaacgga ctcatctgcc acctggtatt ctccctgttg taagcctgca
    24181 agctctgtca tttttcgtgc cagttctgga cccagagttg gggttaaact ggtatcattc
    24241 actcgaaaga caatactagc tatttcatct atattaaaat tcgcagcaag ggagatattg
    24301 gtagtaatag gcaagccacc aaacccatat atttttgacc ttttagcctc cggactagta
    24361 taaaccccaa tgacccggta actaaatcca ttgacttcta caaccttgtt aatagcctct
    24421 tgaggagatt caaataaact aatggacaat tcctcatcta gcaaaataac acttgcaaac
    24481 tctttgaaat cttgctctct cagactacga cctgcaataa tttcattctt aacagcgtcc
    24541 atgtaagttc tgtttccacc tgtcaaatta gcattctcaa cctttttatc ttgataggtc
    24601 aagatggcat tcgttgaatt ggttacatag taactatcca ctcccttcag tttagctgcc
    24661 tcttggaccc aggattcttg cggttttggc ggttcaacag gaatttcctc ttcctttcca
    24721 gaaaccgtaa aagatgattg tttctgagta aaagaaccat ctttactttt tttaggagag
    24781 aaaaagacgc taatattttt ctgagattta gtcatatctt tattgacttg acgagatagg
    24841 gaatcaccca aagccataat cacaacaact gatgaaacac cgataataat cccaatcata
    24901 gtaagcaaag aacgcatctt gtgagccatg atagatgaaa aggcaaattt cagattctgc
    24961 atcttagttt tcctcctttc ctaactgagc actgtcagac gaaatgaccc catcccgaat
    25021 gacaatctga cgtttagcat aggcagcaat ctcaggctca tgcgttacca tgataatggt
    25081 ttttccttct ttattcaaat caaccaataa ttgcataatt tggttacctg ttttggtatc
    25141 caaggctcct gtcggttcat ccgctaggat aatagaagga ttgtttacca aggcacgcgc
    25201 aatggctaca cgttgctttt gaccaccaga taattctgaa ggtaaatggt gactacgttc
    25261 tgtcaattca accttgtcta aatattcctc agccaacttg cgacgttttg aagacgaaac
    25321 tcctgcgtaa atcaagggca attctacatt ttgcagagca ttgagcttcg atagaagaaa
    25381 gaactgctga aagacaaaac cgatttgttg gttacggacc ttagccagtt gtttttcacc
    25441 aagcccagcc acttcttgac cttcaagata atattctcca ctggttggtg tatccaacat
    25501 gccaatcgta ttcatcagag tggacttacc agacccagat ggtcccatga tggctacaaa
    25561 ttcaccctca ttcacttcta gattgatatt tttgagaacc tgcagttctt ggtcaccatt
    25621 acggtagctt ctgaagatat tttttagact aattagttgc ttcatcagcc ttcacctctt
    25681 ttccttcttc caaggaagat gttggattac tgatgacctt agcaccattc gttaaaccag
    25741 aagtgatttc ttgattttct gcgtcagcat ttcccaatga aacctcaact tttttagcct
    25801 tttgttgttc atccacaatc cagacataat ttttactatc atccattact agactgctaa
    25861 caggaacaag aatagcctta gttttgcttt taacctcaat gttgacagaa aaaccctgtt
    25921 tcaaatcacc aacctcgcct gtcacatcaa tagtataagg gtatttagaa cctgtattat
    25981 tcccggctgc tggactagct gcttcaccat tgtttttagg atagtcagaa atatagctta
    26041 atttcccagt ccatttttta tcaggataca ctttagaagt aaagcttact tcttgaccta
    26101 cagaaaggtt ggctagattg tactcagaca attctccctt gacttgtaaa ttttcattgc
    26161 tgacaatatg aaccataact tggctcgccc ctgttggaga tttagaaaca ttgctattga
    26221 cttcgactac agttccctct agggtactga gaacagttgt tgcatccaat tgactttgag
    26281 ccttgcttaa ttgcgctgca gcatctgcac gcgcatcacg ggcatcaccc aattgagcgt
    26341 caatagaagc aacagaattt ccagccactg gagttgggct ttgcaccgtt gcatcttctc
    26401 ctcctactgg cgctggtaac tgtggagccg gagctgaagc ggcttcattt cgtgcttgat
    26461 tgagttcatt gatatgacga tctgccttag ctactgctcg actagctgaa tcataggccg
    26521 cctgcgcttc tgaactactg tacttgacta aagcctgccc ttcgctgacc ttatcgccca
    26581 cagaaacaag gatttcatct aaatcaccct tactagcatc aaaataaaca tattgttcat
    26641 tttttgctgt tactgtccct gacaataaaa cagaggaggc cacgcttcct tccttggcaa
    26701 caacaagatg agtaggctca tcttttagag cagtctgaga aggttgtcta aagagtaaaa
    26761 tccccccagc acccaataca actacactcg cagcaccgat tgctgcatac agttgccact
    26821 ttttagcttt accattcttt ttcttcataa tgaaactcct tttctttttt acaatacttt
    26881 gctattatac caaatttccc tccagcaaac aatacagttc aggattaaac aatcgttcgg
    26941 aattttgctt ttctattgta ctagatatat aatacactat cttgacttat ttggcaagct
    27001 tttttgcaat tttttgtgcg tagcagattt ttgcaatcaa atcaaaaaaa gaataaaata
    27061 taactatcaa aaaagacgct ctactattta aaagattaca aaggagtttt caatgagaga
    27121 atatgatatc attgctatcg gtggaggtag cggaggaatc gctaccatga accgtgctgg
    27181 tgaacatgga gccaaagcag ccgttattga ggaaaagaaa ttaggtggaa cctgtgtcaa
    27241 cgtcggttgt gttcctaaaa aaatcatgtg gtacggggcg caaatcgctg agactttcca
    27301 tcaatttgga gaagactacg gctttaagac tactgatctt aactttgact ttgcaaccct
    27361 acgtcgcaat cgtgaagcct acattgatcg cgctcgttct tcttatgatg gtagttttaa
    27421 acgcaacggt gtagacttga ttgaaggtca tgctgaattt gtagattctc atactgtaag
    27481 cgtaaatggt gaactgattc gtgctaaaca tatcgtgatt gctacaggtg cccatccaag
    27541 tattcctaat attcctggtg ctgagctagg tggctcttct gatgatgtat ttgcctggga
    27601 agaacttcca gagtcaattg ccattctagg cgctggttat atcgccgttg aattagctgg
    27661 cgtactccac acttttggtg tcaagacaga tctctttgtt cgccgcgatc gtcctttacg
    27721 tggttttgat tcctacatcg ttgaaggttt ggtcaaggaa atggaaagaa caaacttacc
    27781 acttcacact cacaaagtcc ctgtcaagtt agaaaaaact actgacggca ttaccattca
    27841 tttcgaagat ggtactagtc acacagctag ccaagttatc tgggctacag gtcgccgtcc
    27901 aaacgttaag ggcttgcaac ttgaaaaagc tggagtgact ctgaacgaac gtggctttat
    27961 ccaagtggat gaataccaaa atactgttgt tgagggaatc tatgctctag gtgatgtaac
    28021 gggcgagaaa gaactgactc cagttgcaat caaggccgga cgtaccctat ctgaacgtct
    28081 ctttaacgga aaaactactg caaaaatgga ttactcaact attccaactg ttgtcttttc
    28141 acaccctgct atcggaactg ttggtttgac agaagagcaa gctattaaag aatacggtca
    28201 agaccaaatc aaggtttata aatcaagctt tgcatctatg tactctgctt gcactcgcaa
    28261 ccgtcaagaa tcccgtttca aactcataac agctggttca gaagaaaaag ttgtcggact
    28321 tcatggaatt ggctacggcg ttgatgaaat gattcaagga tttgccgttg ctatcaaaat
    28381 gggagcaacc aaggctgact ttgatgcaac tgtggcaatt cacccaactg catctgaaga
    28441 atttgtaacc atgcgttaat cgaaacaaaa gaagcaaagc aaaaaacact tctaaatatc
    28501 aaaaagcgag tatttccgta gttggagata ctcgttttct tatgttttat tttatagtat
    28561 gttgaagtaa gatatgcaaa aatcaattaa caaaccata
//
LOCUS       DAVKJD010000017        27494 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKJD010000017 DAVKJD010000000
VERSION     DAVKJD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.017"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(95..730)
                     /locus_tag="VW157_000818"
     CDS             complement(95..730)
                     /locus_tag="VW157_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9552148.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(732..1559)
                     /locus_tag="VW157_000819"
     CDS             complement(732..1559)
                     /locus_tag="VW157_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9552149.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINTLFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(1581..2794)
                     /locus_tag="VW157_000820"
                     /pseudo
     CDS             complement(1581..2794)
                     /locus_tag="VW157_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            3146..3757
                     /locus_tag="VW157_000821"
     CDS             3146..3757
                     /locus_tag="VW157_000821"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9552150.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            3802..4530
                     /gene="rlmB"
                     /locus_tag="VW157_000822"
     CDS             3802..4530
                     /gene="rlmB"
                     /locus_tag="VW157_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9552151.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENSLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(4569..5480)
                     /gene="trxB"
                     /locus_tag="VW157_000823"
     CDS             complement(4569..5480)
                     /gene="trxB"
                     /locus_tag="VW157_000823"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9552152.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(5546..5770)
                     /locus_tag="VW157_000824"
     CDS             complement(5546..5770)
                     /locus_tag="VW157_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9552153.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(5848..6591)
                     /locus_tag="VW157_000825"
     CDS             complement(5848..6591)
                     /locus_tag="VW157_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9552154.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(6591..7391)
                     /locus_tag="VW157_000826"
     CDS             complement(6591..7391)
                     /locus_tag="VW157_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9552155.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(7549..7905)
                     /locus_tag="VW157_000827"
     CDS             complement(7549..7905)
                     /locus_tag="VW157_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9552156.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(7899..8429)
                     /locus_tag="VW157_000828"
     CDS             complement(7899..8429)
                     /locus_tag="VW157_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9552157.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWSYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(8429..8923)
                     /locus_tag="VW157_000829"
     CDS             complement(8429..8923)
                     /locus_tag="VW157_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9552158.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            9058..9504
                     /locus_tag="VW157_000830"
     CDS             9058..9504
                     /locus_tag="VW157_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9552159.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            9501..10148
                     /locus_tag="VW157_000831"
     CDS             9501..10148
                     /locus_tag="VW157_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9552160.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(10169..10750)
                     /gene="pdxT"
                     /locus_tag="VW157_000832"
     CDS             complement(10169..10750)
                     /gene="pdxT"
                     /locus_tag="VW157_000832"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9552161.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(10751..11626)
                     /gene="pdxS"
                     /locus_tag="VW157_000833"
     CDS             complement(10751..11626)
                     /gene="pdxS"
                     /locus_tag="VW157_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9552162.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVESVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(11778..13157)
                     /locus_tag="VW157_000834"
     CDS             complement(11778..13157)
                     /locus_tag="VW157_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9552163.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTNYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            13451..14374
                     /locus_tag="VW157_000835"
     CDS             13451..14374
                     /locus_tag="VW157_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9552164.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            14434..15039
                     /locus_tag="VW157_000836"
     CDS             14434..15039
                     /locus_tag="VW157_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9552165.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            15058..16302
                     /locus_tag="VW157_000837"
     CDS             15058..16302
                     /locus_tag="VW157_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9552166.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGNADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(16419..16676)
                     /locus_tag="VW157_000838"
     CDS             complement(16419..16676)
                     /locus_tag="VW157_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9552167.1"
                     /translation="MDPKKIARINELAKKKKTEGLTSEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(16718..18754)
                     /locus_tag="VW157_000839"
     CDS             complement(16718..18754)
                     /locus_tag="VW157_000839"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9552168.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIHSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(18966..19883)
                     /gene="glyQ"
                     /locus_tag="VW157_000840"
     CDS             complement(18966..19883)
                     /gene="glyQ"
                     /locus_tag="VW157_000840"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9552169.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(20251..21012)
                     /locus_tag="VW157_000841"
     CDS             complement(20251..21012)
                     /locus_tag="VW157_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9552170.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFFTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTLPLLNWFVQQKLIEKEI"
     gene            complement(21285..22127)
                     /locus_tag="VW157_000842"
     CDS             complement(21285..22127)
                     /locus_tag="VW157_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9552171.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYHAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLSHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(22241..23632)
                     /locus_tag="VW157_000843"
     CDS             complement(22241..23632)
                     /locus_tag="VW157_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9552172.1"
                     /translation="MDKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKSFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(23867..24244)
                     /locus_tag="VW157_000844"
                     /pseudo
     CDS             complement(23867..24244)
                     /locus_tag="VW157_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            24477..25454
                     /locus_tag="VW157_000845"
     CDS             24477..25454
                     /locus_tag="VW157_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9552173.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSNRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            25451..26533
                     /locus_tag="VW157_000846"
     CDS             25451..26533
                     /locus_tag="VW157_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9552174.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYIHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(26597..27494)
                     /locus_tag="VW157_000847"
                     /pseudo
     CDS             complement(26597..27494)
                     /locus_tag="VW157_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         8039 a         6208 c         4932 g         8315 t
ORIGIN      
        1 tttacattca taaattcctc cgtcactttt tattttaaag gattttagaa ttttttacaa
       61 gatattatgc tcaaatttaa cattatttag ctatttagta aagatagtct ttcaaggctt
      121 ttgacaaagc ctgataacca gcaatactga ggtgcagtcc atcagttgtg tattcttttt
      181 tgagttggcc tgcttggtct gtcaaacaat caaatactgg cacaaattcc acctgcatat
      241 aggcagatgc aagctcttga taggcttgat tccagttctg aattttttca ttcgagcgga
      301 tatagactgc ctgctggtac tcctctccct cattgacagg caaaatggaa agcaatttaa
      361 tctctgtcaa tggataatcg cgagcaacgg attgaatgat agcttcgaga ttattgagag
      421 cctcattcac aggaacatcc tttccgatat catttgtccc aatcagaaga aaaattttat
      481 ctactgctcc accatataga tgagcatcaa ggttctctag taacagtcct gtctgatagc
      541 cacgaattcc tcgattgaca atcgtctttg aagtcccaaa tagctcctgt agaggataat
      601 actcgacaat ggaatcccca ataaaaagaa tgtttggttc tacaacagaa atgtgattta
      661 ggtgacgata cttggtttga attttttctt gttcctttag gagccaattt tctaataact
      721 gtactgccac ttcattctcc tttttctaac cagttttcca agacttgata aactcctcct
      781 tggctgttgg ctggtgctag agcagtcgcc acagctttgg ctttctcatc agcattttcc
      841 atcgcatagg caattccagc catttcaagc atttcaacat cattttcact atcaccaaaa
      901 gccatgattt cttgggattt caagtcccag cgcttgagta attcctccaa gccccatgct
      961 ttatgaatcc cagcttggag gatatcaatg caaccatagc cactggatac agctcggaca
     1021 cggccatcaa agagagtatt gatttcttcc aaaaccgaac tcaaacgttc ctcaccaaca
     1081 accatgctca tcttgagcac accaccaaag aggtcagatg ttaattcatc cacaaattgc
     1141 atccgttggt agaatttttc aatcatttct ggagtcataa aactttcaag atctgtaaaa
     1201 atcgtacctt ccttgacaaa atcacccttc atccccgtta caacaaactg gtcctgacac
     1261 gctcgaccct tgaaatgagc caaagccttg ttgacaatgg cgtcatccca tgtctgagcc
     1321 tgaatcaatt cattgttttc aaaaatacga gcgccattag caacaaccag aaccactcga
     1381 tccaccaagg gactcagtag ttgtctcatg cggtgaattt cattgcccgt cgcaatgaca
     1441 aaacgaatgc ccctttgatc caactgatct aaaatctttt ccaatcgtgg gagatcaagc
     1501 tgacctctag catccagcaa ggtcccatcc atatctgtcg caattacctt aatcatcatt
     1561 tactttcctc tggattcaag ctaataccac ttcaatccca catgttcatt catttcttta
     1621 taggctgtat caattcgttc cttagtcgct tcatctaact ggtcacgatg actgccatcc
     1681 tctataaaat cttccaaaat ataagtttgg taatgataca agggatatcc ttctggtgaa
     1741 aaggttccct ttggtgttcg attgacccaa gtaggatgat ctttagctgt tccgatagtt
     1801 gtttttccat cattcttctt gatggtgaca tccatgagaa caccacgttc agtccactta
     1861 gcattctctt catctcccat agattcaatt cgttgattgg aaatgaagtt ccccattgaa
     1921 tagataatga gtttcttatc tccatctttt tcaaccgttt cagatggttc aacaacgtga
     1981 ggatgccctc caaagataat atccgctccc caatcgatca tcttgtgata aagagctttt
     2041 tgttcttcag ttggttccaa tcgatactca acacccatct gaggcatgat aatggtgata
     2101 tctgcttcct tctctgcccg ttcaacttca gccttcatct tatcttcgtt taaatctaaa
     2161 agataacgat tatagtcttc ctgagaaata tactgctcaa ttccattgaa accataggaa
     2221 taggctgaca atgcaacctt gataccattc acttccttaa tgaccagcgg aacctgatca
     2281 cgtggttcgt gcgtataaac tccgattgga gtgattccag ctttctcaat aatatcggcc
     2341 gttgaaataa ctccctcaat ttgcgaatcc aaaatatgat tatgagctaa atccagcaca
     2401 tgataacctg cctccttaat agcatccata acttcagcag gagcattaaa gagaggataa
     2461 cccgctaaat aatgatcctt attaatggtt tcttcaaaat caccaatagc taaatctgct
     2521 tgcttgaacc aaggagtcac atactcaaaa ttttcatgaa agtcataggt accgtcttct
     2581 tttttagctg aaaagaaaag tccatcgtgg tagagcaaat ccccgtgggc cataattctg
     2641 gcagtttttt cctcttccta ctcctgagac ttttgcttag ttccctcttg agaaatagta
     2701 tctcgttttt gactagtcaa gagattcctt ggaagctttc aaacaacaag accaagccca
     2761 ttgataaacc aactgctagc aagagtatcg ccacaaatcc cttattgctc cacttgcgat
     2821 aactcctaaa aagtttacca agcccttcat aaaacgaaaa gctaaaccac cctgatttcg
     2881 attttgtctt ctttgtatct tcgttctccc tactttctta tgcaagcctt ttctttttat
     2941 tatatcacag ataagtattt ctttcacaat tgaattgaac ttcccatcta ttttctataa
     3001 atcctaaatg ccataatgct ttcaattctt gtcattttgt gatatcatgt agaggaaatg
     3061 aactaatcca cagtggctta ttccaagtat accacttggg ctttggcagt agctaactgc
     3121 actaaacata atataaggag agaaaatgtc tgcaatagaa cgtattacaa aagctgctca
     3181 cttaattgat atgaacgata ttatccgcga gggaaatcct actctacgcg cgattgctga
     3241 ggaagtcact ttccccctat ctgaccagga aatcatccta ggcgaaaaga tgatgcaatt
     3301 ccttaaacat tcccaagatc ctgtcatggc tgaaaaaatg ggactccgcg gtggtgttgg
     3361 actggctgct ccccagttag atatctcaaa acgcattatc gctgttttgg tacctaatat
     3421 tgttgaagaa ggcgaaactc cacaggaagc ctacgatttg gaagccatta tgtacaatcc
     3481 aaaaatcgtc tctcactctg ttcaagatgc tgctcttggc gaaggagaag gttgcctgtc
     3541 tgttgaccgt aacgtgcctg gctatgttgt tcgccatgcc cgcgttactg ttgactactt
     3601 tgacaaagat ggagaaaaac accgtatcaa actcaaaggc tacaactcca ttgttgttca
     3661 gcatgaaatt gaccacatta acggtatcat gttttacgat cgcatcaatg aaaaagaccc
     3721 atttgcagtt aaagatggtt tactgattct tgaataaaga aaatcccgtt gcaagacggg
     3781 gttttgtgtt ataatagagg catgaaaaca aatgatattg tctatggtgt ccacgccgtt
     3841 accgaagctc tccttgcaaa tacaggaaac aaactctacc tccaagaaga tctccgaggt
     3901 aagaatgttg agaaagtcaa ggaactagct acagaaaaga aggtgtccat ttcttggaca
     3961 tcaaaaaaat ctctctctga gatgactgaa ggtgctgttc atcaaggttt tgttctacga
     4021 gtgtctgaat ttgcctatag cgagctagat tacatccttg caaaaacacg ccaagaagaa
     4081 aattcacttc tattgattct agatggtcta accgatcccc ataatctggg ttctatcttg
     4141 cgaacagccg atgcgaccaa tgtttcaggt gtcatcattc ccaagcaccg tgctgtcgga
     4201 gtaactcctg tcgttgccaa aacagccaca ggtgctattg aacacgttcc aattgcccga
     4261 gtgacaaacc tcagtcaaac cttagataaa ctcaaggatg aaggcttctg gacttttgga
     4321 acggatatga acggtactcc ttgccacaag tggaatacaa aagggaaaat cgccctcatc
     4381 attggaaatg aaggaaaagg tatctctagc aacatcaaaa aacaagtcga tgagatgatt
     4441 accattccga tgaatggaca tgttcaaagc cttaatgcca gtgttgctgc ggccattctc
     4501 atgtacgaag ttttccgaaa tagactataa aaaagtttcc agtcatctga ttggaaactt
     4561 ttttatgatt aactatgttc tgtgataaac ttgtaggctt cttgaccagc gatagctcca
     4621 tctccaactg ctgttgttac ttggcgaagg tctttcaagc gaacatctcc aactgcaaag
     4681 ataccgtcga ctgcagtctt catgtggcta tctgtcacaa tccagcctgc ctgatcttgg
     4741 atattcaatt ctttaacaaa atcgctaaga gggtccaaac caacatagat aaagacacca
     4801 ccgaaggctt gttctgtcac ttgacctgtt ttcacatttt caaatacgac tgattctact
     4861 cggttttcac ccttgatttc ccttactaca gaatcccaga taaagctgat tttttcattc
     4921 gcaaaggcgc gatcttgtaa aaccttttgg gcacgaagtt ggtcacgacg gtgaacaatg
     4981 gtaacagtct tagcaaaacg agtcaagaag agggcttctt caacagctga atctccacca
     5041 ccaactacca ataaatcttg gtcacggaag aaagcaccat cacacacagc acagtaagaa
     5101 acaccacgac tgttcagttc ttcttctcca ggcactccca aaggacggtg tttagaacca
     5161 gttgctacga taactgtacg tgtttcatat gtttggtcat cagtcatcac tttcttaaaa
     5221 tcaccatggt cttcgacatt ttcaacataa ccataaatgt gctcaacacc aagattttca
     5281 agtggttcaa acatcttttc agccaattca ggtccactaa tattagcgta tcctgggtaa
     5341 ttttcgatat cagatgtatt attcatctga ccacctggca gaccaccttc aatcaaagct
     5401 acttttagat tgcttcgagc agcatacaag gccgcagtca tccctgcagg tccagcaccg
     5461 ataataatag tatcgtacat atagattcct tctttcttgt tgtaactatc tttattctaa
     5521 ctctttcttg tcaatcacgc aaggattatg ccttgaaaac aagaattaaa ctcataaccg
     5581 caaaggataa tactgggaca accaagactc caatcataat catatcatag agatgggctt
     5641 ggcgagcctt ggctgtctta tcaactcccg acatggctct cagaccaatc caaatcccta
     5701 aaaaaatcag aacaaggatg gtggtcaaga tcaaactctc gaaatataaa gaaaatagtt
     5761 gcagtagcat gatttctctc atttctatct tttttaaaga gtaaactcag ctagtccaac
     5821 taactgagtt ttcctttatc tattatatca aatataagtc cgtttgtaac tagcaaagaa
     5881 ttcttttgtc cgctcttctt taggggtgtg gataatctca tccggagttc cagactcgat
     5941 gattttcccc ttatctaaga agagaatctt atccgcaact tgggctacaa aggacatgtc
     6001 atgactgacc aaaatcatgg tctgacctga cttagcagca tctgcaatag acttttctac
     6061 ttcaccgacc aattctgggt caagggctga agttggttcg tctaagagca aaacatctgg
     6121 tttcatagca agcgcacgcg ctagggcaac ccgttgcttc tgtccacctg ataaatggcg
     6181 aggataatgg ttttcacggt ccgaaagccc aaccttagcc aactcttcct tggcaatctt
     6241 agtcgcttct tggtcagata atttcttgac aacaaccaag ccttctttca cattatcaag
     6301 tgctgttcgg cgttcaaata aattaaactg ttggaaaacc atagacaact tacggcgtag
     6361 ggcaaggatt tcttcttgag tgattttaga aaaatcaact gaaaaaccat caatctgaat
     6421 agagccactg tcaggtgttt caagataatt gagactgcga agaaaggttg attttccagc
     6481 tcctgaagaa ccaatcaagg ctacaacttc ccctttttga atatccaagt tcagatgatc
     6541 caagacagtc tgtcctgaaa aggatttgct taaattcgaa atcttaatca ttaacgaagg
     6601 tctcctttca catctgtttg cactgtatca ggtgcagaaa tagccatttt tctctcgatg
     6661 aaacgaccga ggctttcaat tccgatattg actacccaat aaacaagggc aacggagatg
     6721 aagcgttcaa aatagcgata atcagctcca cctagaatct gagcttgggc aaagacttcc
     6781 acaacacccg cactaaaagc tagagatgtt cccttggtca aaccgatgag ggaattaatc
     6841 aaggttggag tagctaccac cgctgcatta ggaataatca ctcgtcgata aacttgcgct
     6901 cgggtcatac ccagactgcg tgccgcctca atctcaccag gattaactga gagaatggct
     6961 gcacgaatgg tttcactagc ataagctgcc tcattaaagg caaaggcgac aatcgcaaaa
     7021 gctgcagctg gaatcgcatt gatattgaga ccagttcccc attgctgatt gagggctttc
     7081 aaagccaaag ggattccgta gtaggtcaac atgagttgca ccaaaatcgg tgtccctttt
     7141 aagaaactaa caaagaaggc ctgcaaggga tataaaatct tgacacgatt gatcttcaca
     7201 atggcaaaaa gaagcgccaa aaccaagcca aaaagggcac caccaattgt caacataatt
     7261 gttgttggaa gttgttggac aattctaggg attccatcaa agaccgaacg taggctaaac
     7321 agcttgccat ccggaatcaa ttgcatcaag ttttggtacc aatctgatgc taaaatcgtt
     7381 gtaacattca taaaaacatc ctcttctgat aatgattcat tctaccatgc ttctgccgtc
     7441 aatgaaatta tttgctacgg tcagatacag actttgtcta cctactagtt tatcatactt
     7501 tgaaacaggg aagttatata ttttatctat caaagagata gataaaaact atttcagtcc
     7561 cagttcttca taagattttc gataaccgat ttgcttaaca gttccatttt ctactaaaat
     7621 gggccgtttt aacaacatac cgtcacttgc tagcaactca gccgcttctt ggtttgacaa
     7681 acttcctacc ttatctttta gccctaattc acggtatttg ataccactgg tgttgaaaaa
     7741 ttgcttcaat tcaaatcctg aggtttctag ccaattcaaa atgacttctt ggctaggtgt
     7801 ttcttccacg atatggacgg ctttatagtc cacacctaat tgatttaatt cttgttttgc
     7861 ttttttacaa gttgaacatt tggggtattc gataaattct aacatgtgct tctccttcta
     7921 ttgtttctat ctttaaaatc tactccttca tgctccaaga gccaagcttt cttttccact
     7981 cctgcagcat agcctgtcag acgcttgcct gctcccaaca cacgatgaca aggtactagg
     8041 atagaccaag gattgcgtcc cactgctcca ccaattgctt gagcagaagc cacttgcagg
     8101 tcttgagcaa tttgtccata ggtcactgtc tgaccataag gaattccctg taaataggac
     8161 caaactctct tttcaaaatt cgttccaatt ggcgccaaga gcaagttgga taaatcctga
     8221 ggcttgcctt taaagtaatc atctaagcaa gcaataactg ggtctaaaat aggatgacta
     8281 acaacttctt ctatcgtttc atctcctagt cccctctcaa aatgcttctg ctcctgaacc
     8341 caaattccat acaaataatg gtcatcagct acaagtgata gaataccaat tggtgaggag
     8401 tagagtattt ttacatactt cttttgcatt atttctttaa tttttgatag gccaagcgtt
     8461 cgccatctac tggcacctta ccaacttgtt taaaaccaag tttttcaaaa atatgttgca
     8521 taaccttgtt ttcagcatgc gtatctgagc gaaaatcaag ataatcaaaa ccttcaatca
     8581 agccctctaa gaaggtttga gcaactcctt ttccctgcac atctgctgcc acagcaatac
     8641 ggtgaaagac tagatactct gactctccag cttgccagtt tccctcataa atagcttcat
     8701 aggcctcctc tggactcttg gtcacagcag cataggctag cagttctccc tcttccaagg
     8761 ctacgtaggc ttgccctgag atgatatcat cgataataac atctgcattt ggatagccat
     8821 tttgccactg gtcactacca gcatctgcta aacatttttt ggcatcctcc atcacctgca
     8881 tgatggcatc tacttcattt ggaaaagcta aacgaatttc catctttttt cctcatttct
     8941 gactagtttc tcccatcata gcataattta agtatttcta caagcagtta aaacttgact
     9001 ttgtgttttt gttattttat aatctagtta tcaaaactat ataaaaagga gttgaaaatg
     9061 aaaactacct tttcctaccc aaaatgggca gaaattccaa acattgacct ctatctggac
     9121 caggttttgc tctatgtcaa tcaggtctgc gcccctatct ctcctaataa agacaagggc
     9181 ctaacagcat ctatggtcaa caattatgtg aaaaatggtt acctgacaaa gcctgacaaa
     9241 aaaaaatacc aacgccaaca gattgcccgt ttgattgcta tcacaaccct caagtctgta
     9301 ttttctattc aagaaatagc ccagacactt aatactctac aaactcaagc aagttcagac
     9361 caactctacg atgcttttgt ggactacatg aaccaaggaa ttgacccagc taaccctatt
     9421 atccaaacca gctgccaaac cgttaaactc tatcatcaaa ctctagactt aatcgatcat
     9481 actcaagagg aggtaatcca atgaacacta gtcttaaact cagcaaacaa ctcagttttg
     9541 gagaggagat tgctaatagc gtgacccatg ctgtgggtgc agtcatcatg cttatcttgc
     9601 tgcctatttc atccatctat agttatgaag cacacggatt tttatcctct atcggcgttt
     9661 ccattttcgt catcagtctc tttctcatgt tcctatcatc caccatttat cactctatgg
     9721 cctatggttc gacccacaaa tatgttttgc gaatcattga ccattctatg atttacgttg
     9781 ccattgccgg ctcatacacg cccgttgtct tgaccttgat gaataactgg tttggctatc
     9841 tgattattgt catccaatgg ggaacgacca tctttggtat tctctataaa atctttgcta
     9901 aaaaggtcaa tgagaaattt agccttgctc tttacctgat tatgggctgg ttggttctgg
     9961 ctatcattcc tgccattatc agtcaaacga cacccgtttt ctggagtctc atggtaactg
    10021 gcggactctg ttatacagtt ggagctggat tttatgccaa gaaaaaacct tatttccaca
    10081 tgatttggca tctctttatc ctagctgcgt ccgcacttca atacatcgct attgtttatt
    10141 acatgtaaaa aagttgagaa gttcaatctc aacttttttc tttacacata ttgataaagt
    10201 actggtgcaa gcgcacatca tcagtcaatt ctggatgaaa agaacttacc aacatatttt
    10261 tttcttgggc tgcaacaatt tgattgttca ctgttgctaa aatttctaca ccctcaccaa
    10321 cactactgat aatcggacca cggataaagg tcattggaat cttgccaact cccttacatt
    10381 ctgcttccgt gtagaaactt cctaattggc gcccataagc attacgctcg accaccatat
    10441 ccatagttcc tagatgactc tctttctgag aagtgatttc cttagccagc aaaattaagc
    10501 ccgcacaggt cccaaacact ggtaagccag atagaatggc ttctcggatg ggaagtagca
    10561 tgttctggtc acgtaagagc ttgcccatgg ttgtagactc accaccaggc aaaatcaaac
    10621 ccgacaagtc actctgatct tgctgaaaat catctagatt tctgagttct acactctcga
    10681 cacctaattg atctagcact tttgcatgtt ctgcaaaggc cccttgcaag gccaatattc
    10741 caattttcat ctattttcct cgttcagcca tgagaatttg gatttcattt tcattaatac
    10801 caaccatggc ttctcctaaa tcttcagaga tttgagctag gatttgagga ttacggaagt
    10861 tagtcacagc cttaacaatg gcactcgctc gtttaacagg atctcctgac ttgaaaatac
    10921 ctgaaccgac aaagaccccc tctgccccta attgcatcat taacgcagca tctgctggcg
    10981 ttgcaacacc tccagcagcg aaatttacaa ctggcaattt tccatgttca tgaacatatt
    11041 ggaccgattc tacagggact tgcaaatcct tggcagcaac ataaagctcg tcctcacgta
    11101 agttttgaat gcggcgaatt tcctgattca tcatacgcat atgacgaaca gcttggacta
    11161 tatcccctgt ccctggttct cctttggtac gaatcatgga agcaccttca gcgatacgac
    11221 gcaaggcttc acccaaatcc ttagcaccac agacaaaagg aacttggaat tctttcttgt
    11281 ccacatggaa acggtcatca gctggagata gaacttcact ctcgtcgata taatcaattt
    11341 caatagcctc taaaatctga gcttcaacaa aatgcccgat tctgacctta gccattactg
    11401 gaatactaac cgcttcttgg atttccttaa tcatctttgg gtcgctcatg cgggaaactc
    11461 ctccagctgc acgaatatca gccggaattc gttccaaggc catcacagct gccgcaccag
    11521 cagcttctgc gatacgagcc tgttcaggat tctgcacatc cataataaca ccacccttga
    11581 gcatctgtgc caagttttta tttagttcat aacgattttc agtcatttgt tttatcctca
    11641 tcatttgtat tggtagctac cttttcattt taagtcagaa cagaatgaag cacaagataa
    11701 aaaaactata attgtatggg gacaaattag ctaaaaacta tctgacctta acttaaggcc
    11761 agatagctca ttcattttta tttttcagcc gtaagggcag ccattgtgat gtagttgtat
    11821 ggtttgttga agtgtggcaa gaagaagagg tctgtcaatg ccaatttatc aattgtcaca
    11881 tgctcttgga tagcaagtga gaacatgtgg attcccatgc taattgcaat atcatgtgaa
    11941 accatttggg caccaagaat ttcacggcta tctttgtcaa agacaatctt aattgctact
    12001 tcatggttgt catgtttcat gaattctggt ttttgaagat cgttaaagcc tgtttcagtt
    12061 gcgttgtaac cagcagcttt cgctttttca agagtcaaac cagttgaaac catgtgaaga
    12121 ccgtagattg agataccatt tgaaccttga acaccgattc cttccaattc atgtccacaa
    12181 gcgttgtagg caccaacgat accagtgcga acagcatttg aagcaagagc gatatagttt
    12241 gtatctttac gagcattgtc ataaacagtc gcacagtcac caacagcgta aacgcctggg
    12301 atagatgttt cttgtttctt gtctacaagg aaggcaccgt tgcggaagag ttcaatttta
    12361 ccatcagcaa gggctgtgtt tggacggaaa ccaactgcaa ggataaccat atccacgtca
    12421 aagctttctt tgtcagtaat caagcgttca actttaccgt caccttcgat tgctttaaca
    12481 gtttgaccta gagccaagcg gatgttgtga tcttccaagt tcttcgccat catttgtgtg
    12541 aagtctttgt catagtaacc gttcaagaca gtatcaacga tatcaacaag gacaacttct
    12601 tttccaagac gttcaaaggc ttcagcaagt tcaacaccga tgtaaccgcc accaacaacg
    12661 gcgatacggt cgaggtgttg gctcttgtca gaaagtttat tgataacttc ttcagcattt
    12721 tggtacaatt tcacgaattg tacgttttca agagttgctt taaattcgcg gtttccttta
    12781 acaatttcaa caccttcgat tggtggcaag attggtgtag aacctgtagc gaaaatcaat
    12841 ttctcgtatg attctttgtg ctcttttcct tcaacttccg ctgtaactac tttgttatca
    12901 tagtcgattg aaagaacagg tgagttcatg taaactttag cacctttagc ttccaatttt
    12961 tctttatcag aatagaacaa gccttcagca ccgtcaattt gttcaccaat ccaaagagcc
    13021 attccacatc ctaggaaaga gatgttagag ttttggtcaa atacaacaat ttcgttctca
    13081 tttccaaaat tatccaacat ggtattgata catgctgtac cagcgtggtt agcaccgact
    13141 acaacgattt tactcataaa aatttcctgc cttcattcta aatttacctt actagtatac
    13201 catttaccgt tagcgtttac aaggggggta ctattatttc agcatttttt atcaaaaata
    13261 attccccatt tatcctgttt tcaagcctca tgaattttat gagcatattt cttgactttt
    13321 tccaaatttt gtttgtgaaa attctaactt tatggtatta taataacatg aaaatgaaat
    13381 gtaagggctt caatttagat gattatttga tattacaact tattttctat aagagaaagg
    13441 agttggtagc ttgactcttc gttcacattc tgaacggcta atggggacta ctatcactat
    13501 ttcattagtg gatgagcaag ccgatatctt tctccaaaaa tcctttgact tgctcaaaga
    13561 acttgaatac cgcttcaacg ccaatagtca agaatctgag ttgatggaaa tcaattatca
    13621 agcaggaata gcccccgtca cggttcatcc agacctgttt gagctgattt cacttgggtt
    13681 agagcatagc ctagcgctct ctagccacct caatatcagc attggtccct taattcaaac
    13741 ctggcgtatc ggtttttcag atgccaaggt cgcccagcct caagaaattg aatcggtgct
    13801 gcctctaatc aatcctcatg gtatcgagtt agattcttct acttctactg tgtttttaaa
    13861 acagaaagga atgaagatcg atctaggttg tttagccaaa ggatacagtg cggataaggt
    13921 tgcccaattt cttaggaaag agggggtgac ttctgccttg atcaatctgg gagggaatat
    13981 cctgaccatt ggaaaaaatc aagcaagagg ggataaccca tggcaaatcg ggattcaaga
    14041 cccagccaat cctaggggaa atcatttaat gaccatccct gttgtcaata aatctgtcgt
    14101 gacttcaggc atttatgaac gtcacctgac cgtcgatgga caagattacc atcacatttt
    14161 tgacagtcaa acaggatatc ctgttgaaac ggaactagcg agtctaacaa tcatctctga
    14221 taaatcagtc gatggcgaaa tctggacgac tcgactcttt ggagaaagac ctgcttctat
    14281 cctctggcaa gtcgaaagtt tggagggcat cgaagtcatc ctcatcgata aagaaggcca
    14341 cctaagctgt tcttcaggaa ttcctactct atagagacaa ctgtttcaat ggaattttaa
    14401 aattgtatta tgtaaaaaga gaaaggaaat ctcatgttaa aacttattgc tattgttgga
    14461 acaaattcaa aacgttctac aaaccgtcaa ttgcttcaat acatgcaaaa acactttgct
    14521 gacaaagctg aaattgagct tgttgaaatc aaggccattc ctgtcttcaa caaaccagct
    14581 gacaagcaag tacctgctga aatattggaa attgctgcta aaatcgaaga ggcagatggc
    14641 gttattatcg gtactcctga gtatgatcac tctattccag ctgttttgat gagcgctctt
    14701 gcttggttgt cttatggtat ttacccactt ttgaacaaac caatcatgat tacaggtgct
    14761 tcttacggta cgcttggttc atctcgtgcc caattgcaac ttcgtcaaat cttgaatgct
    14821 cctgaaatca aggcaaatgt tcttccagat gaattcttgc tctcacactc tcttcaagca
    14881 tttaacccaa gtggcgactt ggttgacctt gatgttatca agaaattgga tgccatcttt
    14941 gatgacttcc gtatctttgt aaaaatcaca gaaaaattac gtaatgcaca agaattactt
    15001 cgcaaagatg ctgaagactt tgactgggaa aatttgtaag ataggagacc aaaaagaatg
    15061 aaatttgttg gacttgttgg atcaaactac gatcaatcat ataaccgtaa gctcttggaa
    15121 tttatccgtc gctacttcaa actcaaattt gaattagaag ttcttgaaat tgacgaagtt
    15181 ccaatgttta accaagatga aaaatgggat gaaagtttcc aattacgtta tttatataac
    15241 aaaattactc gtgcagatgg tgttatcatt gctactcctg agcataacca cactatctca
    15301 gcttcactca aatctgtact tgaatggctt tcatacgaag ttcatccatt tgaaaacaag
    15361 cctgttatga ttgtgggtgc atcatactat gaccaaggaa catcacgtgc tcaagttcac
    15421 cttcgtaaaa tccttgatgc tccaggtgtt aatgcctaca cgcttccagg taacgaattc
    15481 cttcttggta aagctaagga agcttttgat aacaacggaa atatcaccaa cgaaggcact
    15541 gttaaattcc ttgaaacttg cttagataac tttgttaaat acgtaggagt cgtttcgaaa
    15601 ttgaaaaaac caaaaccaat cgaatcagaa gacttggatt gtggaaaacc aatcgctaca
    15661 accatcacag aagttgaccc tgacgatcca gaatgggtag aaaaagttgc agcaatcact
    15721 ggtgctgttt ctggtgatac ctatgtcaaa ttggaccacg gtatcctaac agttaaccaa
    15781 attgatatgt tcttgaaagc tatgccattt gaattgacat acgctgacga caacaaccaa
    15841 ttcctctact acaacaacgc tcaccaagat ccagacacta tgttcgctaa acgtgtacca
    15901 cctcaatcag gtagccgtat gtcgactgtt catggttctc ttccaccagc acgtatgaag
    15961 aacgtagagt gggttatcgg aacacttcgc aacggaaacc aagaatacgt ccgtacgatc
    16021 gttccaggtt ctcctgcagg tgttatcaac actcacaact accaagcaat gtactatcct
    16081 gatggatcat acgctggtat caatgaaatc gtctttaact tccaaccatg gcttgactgg
    16141 tacctaaaag agactggtca acgtttggtg ggtggtagcg gaccatttgc tcctgctgcc
    16201 ggaggccatg gaaacgccga tgctacttct ggcgcttctg attcagggga tgctggaggc
    16261 cacggtggtg acgcagatgc tacagccggt gcaagttact aatgatagtt tttaggaacc
    16321 attatggttc cttttttagc gaaaaaagac tagcaaacac aaactgctag tctctttgtt
    16381 atcgtttcat ctatctttgt taaaagaaag aatacatatt atgaatttgg gtcatcaaga
    16441 ctacggccat gtaatccttt ttcacgttgt acttggcgta gtttttctgg tgtaacatcg
    16501 tttccttctt cgtccacaat tttgattcct tcaatgtggt gacgaacagc gcggcgataa
    16561 ccttcgatgt actcctcacg tagtttggct tgttccactt tttcttctga tgttaagcct
    16621 tctgtttttt tctttttagc aagctcattg atacgagcaa tttttttcgg atccatttct
    16681 tctcctttgt aataagataa agtccgttta tcaaatttta tttagtgtta atttggttaa
    16741 aacaagcaaa cttagctgct ttcttggtta gttgtgacaa gattgccaaa cgattttgac
    16801 ggacagcctg atcttcagcc attaccatag tattttcaaa gaaagcatca atgactgggc
    16861 taagcgcaaa gagttgtttc aattgctgac ttgcaggtcc tgataaaacg agtgtttcta
    16921 ctgcttctgc caaagctttt tcttggtcat tctcaaatag tgctgaatca actgtagcaa
    16981 ccccttctgc cttctcagcc aggttaaagg cacgagaaag tgattcaaca gatggtttaa
    17041 aatcttcttc cttgcttact tctacgagag cacttgctgc ttccaacata tctgccacaa
    17101 caaagtttga acctgcaaga actgcttcct tgatatcttt tggagtagag cccatcatct
    17161 tatcaacacg agccttgata aagtccataa cctctgcttt attttcataa gtcaaactgt
    17221 caaatttcaa tgcataaagg ctatcaatca gctcatccat agcaatgtgc caaccaaagg
    17281 catccaagat acgaaccaca ccttgagttg cacgacgaag ggcataaggg tcattagaac
    17341 ctgatggaat caatcctact gagaagaaac tcaaaatcgt atccaatttg tctgcaatgg
    17401 ctagaactgc gccgaccttg ctctctggaa gttctccttc agctgatgta ggcatgtagt
    17461 gttcacgaat agcagctgcc accgctggag tttcaccagc aagaagggtg tatttttcac
    17521 ccataattcc ttggagttcg tcaaattcac caaccatacc tgtcaacaag tcaaacttgt
    17581 aaatggctgc tgcacgagca aggtcaactg tttcatccac tgacaaacca gctttttctg
    17641 ccaaaagtac agtgatttga cccgtacgaa tcatgtgttc acgaagggaa ccaatcttct
    17701 catggaaggt gacattgttt aatttttcaa caagatctga aatcaccaat ttttggtctt
    17761 cacgccagaa gaattctccg tcttccaagc gggctaccaa gactttttca tttcctttga
    17821 tgacattttt caaacgctct gcgtttccgt tacgaacaga aatgaagttt ggcaagagtt
    17881 ttccatcttg atcacgaaca acaaagtaac gctggtgttc cttcatagaa gtcaccaaga
    17941 cttcttctgg aacttcaagg tatttagcat caaaacttcc catgaaggca gttgggtatt
    18001 caaccaaatt caagacttca ttcagcaaat ccgcatcaat ttcgatacgt acaccatgtt
    18061 ttgcctcaat ttccttgatt tggtcaacaa tcatttgctc acgttcacat ggatctgcga
    18121 ttacaaactg cttacgaagg tcttcttcat agctcaatgc tgaatgaatt ttggtttctt
    18181 tccccaagaa acgatggcca cggctcacac gacttccctt gatatcaagg aaatccaagt
    18241 caaactcttg ctcatccaag agaacagtta aagtgtggac aggacgaatg tattcaaagc
    18301 tatttccagc ccagtgcatg ctgacaggga aagtcaatga cttcaagaca tctacaacac
    18361 caggaacaat ggcttcaact gcttgaccaa cttcttcctt agtgacatag acatattctt
    18421 cacccttgat ttcacggaat tcgatatctt caacagtcaa gccttttcca cggacaaatc
    18481 cttgagctgc tttggtgaag tttccatcac tatccaaggc aattttcttt gctggtccct
    18541 tgaaatcttc tgtcaaatca gactgtttgt ctgataaacc agtcacacga acagccaaac
    18601 gacgtggtgt tgagaaggtt tgaatggctt caaaagacag gcggttttcc ttgaggaagg
    18661 ctgccatttt ttcgcctagt tgtttttcac ttggtgtgac aacataggct ggtaattctt
    18721 caagaccgag ttctactaat aagttttttg tcatgttttt tctccgaaaa gtatcttttt
    18781 attttccagt ttgccttatg tgattggcac gcaagcaacc aacttcgttg tttcattgct
    18841 aaaattggag ccaaaagtct ccaattttgc ctagtatcct tagtttcact aaggatacct
    18901 tcatcactgt ggcaactggc tcagggctca ctctgttcgc ccattttcgt aatttgtcac
    18961 tctctttatt ctgcgtcttc tgctaaaagt ttaactcgtg tttcttcatc caaaagtggg
    19021 taacctaggc gtttgcgttc tgcaacaaag gttttggcta caacacgggc caagttacgg
    19081 atacgagcga tatagcctgc acgctctgtt acagatacgg caccacgcgc gtcaagcaga
    19141 ttaaaggtat gtgaacattt gagaacatag tcataggcag ggtgtaccaa gccttcttct
    19201 aatgcacgac cagcttcttt ttcaaactta tcaaagtttt caagcaacat ttcttggtcc
    19261 gaaatttcaa atgaatattt tgagtgctca tactcaggct ggataaagat ttctccgtat
    19321 tttacaccat cagcccactc gatatcatag acagagtcta cttcttgaat gtaagaagcc
    19381 aagcgctcca aaccataggt aacttccgca gtcacagggc tagttgccaa tccaccgact
    19441 tgttggaaat aagtgaactg agtgatttcc attccgtcaa gccaaacttc ccaaccaaga
    19501 ccagctgaac cagttgatgg gttttcccag ttgtcctcaa caaaacgaat atcgtgctcc
    19561 aaaggattga ttcccaattt ttccaaagac tcaaggtaaa gttcttggat atttgatgga
    19621 gaaggcttca tgaccacctg gaattggtgg tgttggtaga gacggttagg gttttcccca
    19681 taacgaccgt cagcaggacg acgtgatggc tctacataag ctgcattcca tggctcaggt
    19741 ccgatagcac gaaggaaagt gtaaggactc attgttcccg cacctttttc attatcataa
    19801 gcctgcataa gcatacaacc ttggtcattc caaaattgtt gcaaagtaag gatgatttcc
    19861 tggaaagtca atttcttaga cattgtttac tcctttttat ataaattact tgcgacatga
    19921 gtctgcctcg tcgatcatac gaggcaagac gagttagtac tgcgtctagc tcaggcactc
    19981 tagtgctgag cgtggggcag tccgactgta aggaggtttc tgccactgat gcagtgaaat
    20041 gttaatttct tagacattgt ttactcctta ataaaaaatg atttcttgcg acacgagtct
    20101 gcctcgtcga ttatacgagg caagacgagt tagtactgcg tctagctcag caccctagtg
    20161 ctgagcgtgg ggcagtccga ctgtaaggag gtctctgcca ctgatgcagt gaaatgttaa
    20221 tttcttagac attgtttact cctttagttt ttatatttct ttttcaatta atttctgctg
    20281 aacaaaccaa tttaacaagg gaagagtttt ttctgtattt gcccatagat gataagaatc
    20341 taagaccgct tgtacactac ctaaattttc taccaactta tctactgtca aaaagctgcg
    20401 ccagtattca taaaaaatac ttgcatagtt cttatcataa gtcgaagaac caaaatcatt
    20461 caatgaatgc caaccatact tcttctgaaa aagttctacg agagattgat tacaaatttt
    20521 ttccgcttga aattcctctt ctgtaaagaa atacttgcga ctaatatatt caaccatccc
    20581 ctcttcgaac cagatataag agtcataacc atcaaaatca tctaaaaaat gctccgacca
    20641 atgagctaac tcatgtccta caatctgtaa gaggaaattt tcagataaag aatgatagtg
    20701 actttctatt gcttgagttt gctgagaaca ctcgtagtcc atcaattgat acaaatacaa
    20761 ctctttccaa acagctaaat caggcgccat aaccattcgt ctattatttg tataggctgg
    20821 aacagcgctt tccctaataa tctgtgtagc agcattaaaa tctgaccaaa taacggcttg
    20881 cggtaaatca cagactgcaa gttcattctt taaaaaagat tgataatctt tcaacttttc
    20941 agtatttctt actacaaaat cacgaaatgt agctagttga ctatcgtctt ttacaagata
    21001 aaggttgtcc acggctctcc tttctttcac acaatagact gaataagcaa aagaaccgcg
    21061 tccgacaatc ctaggttgat ttaacctagg ggcgtcagaa acgcggttcc accctaattt
    21121 attacttcat tgtttttgaa aatactacag aaagcgccag ctcattattt tgccctactt
    21181 ggctcccact atcccaagct cgcttgaaga acgccataag actttctttc ctgctgacta
    21241 ttatatcaaa aattagaata atgtacaatt ctttttatga ttttctagaa atccacatca
    21301 tcaactcgtg gagcacccga ttgaacagca atcgttttta agttttctct ctcctcatga
    21361 ctcagttcaa ttccaaagca atcaagatta gcttgaatac gagaggttgt gacagatttt
    21421 ggaagtggta aaaatccttc tgccaagctc caggccaagg ctatctgagc aatcgatttt
    21481 ccgtgatttg ctgcgatttc ttgcacttgc ttgctatcaa atagttctcc ctgaccaaaa
    21541 ggcccccaag cttccaataa aattcccttt tcacgacagt aagctacgac ttgatcttga
    21601 tacacacctg gcgccaagcg aacttgattg accgcaggaa cgatagttgc agtttcaagc
    21661 aaggcatcca aatgatggga aagaaaattg ctaacgccga tagcacggat tttcccttct
    21721 tgatagaggt cttccatcgc tctccaaact tccgcattgc gagttttcca tgcgtcattt
    21781 tctctgagcg gttttgggtt cggccaatga atcaaataca aatccaaata atccaagccc
    21841 agtttttcta tagatttttc caaagcttga cgagtttgct cataggtttg ctgactattc
    21901 caaagcttgg tagttacgaa catttcttca cgtggaactc cgctatcttt gattgcttga
    21961 ccaacacttt cttcattctg ataaatcgcc gccgtatcaa tatgacgata accagccttc
    22021 aaggcttcta acactgcatg ataggcttct tctccatcct tagccttaaa agttccaaat
    22081 cccaatactg gaatttctac tccattattt aattgatagg tattcatatg cttctccttc
    22141 tattttattc atcaattata gcataagaca ttttttaagt agtactatct tctgtaaaaa
    22201 ataagtctgt caaatatcta acagacctat tttttcttgc ttattcatca cgactatagt
    22261 acagctcatg agcttttagg cgagtattga gcatctctgg tatggtcaca aaggtataac
    22321 cttgattttt caaatactca atgacccttg gcaaggcatt gactgtcgga ctgtgaatat
    22381 catgcatcaa aacgatagag ccgttagcta cttgatgctg aatttctgtc aaaatagatg
    22441 cttcattttt actcttccag tccagactat ccacatccca catgataaag ctcaaatcca
    22501 agctattgcg aatatcatct gtaatagcac cataaggtgg acgcatgagt ttagaactag
    22561 aacccagcac tttagttagc acatcctcag tatcagtaat ctgcttttta gcttcatcaa
    22621 gagagagttg cgagagaatc ggatggctcc aactatggtt tccaacaaca tgaccttcag
    22681 attttatcct cttcaccaag tcctcattcc cagaaacatt tttcccaagc acaaagaaag
    22741 tcgctttaat atcatattta gctagggtct ctaatacctg cggggtcgtt gctggatttg
    22801 gaccatcatc gaaggttaga gcgacaactt tttgatattt cttatcaaag taagattggt
    22861 acaaggccgc atctttttcg agtaagtacg aagattggat aacatcaaag aaagcagata
    22921 ctggcaaggc tatctcttct aaattttcaa ccacaggact tggataaagg ataatctgac
    22981 tatccttgta atcaaaattc catgcagaca agtcttggtc agagaagctt tttacaatct
    23041 gctcactctg gtcttgctct atttttttat cctctatgaa ggaggtcaac tcttttatca
    23101 gctgttcctt agccttacta gcatctgaaa acagttggtc aagtgtaaaa ggttgcccat
    23161 cttctgttaa gtgaaccttt cctagactgg tcttttcagt ctcttcaacc ttaaaagcaa
    23221 ctaaatcata gatttgtttg gtcacattac gattaacgac tccctttaaa cctgactctt
    23281 cttgctctgt atagtagaaa acaagattgt ccttactttc tagcttgtcc ttaacatctt
    23341 gatttatcaa ctccctaaca gaggaaatca ctttctcccc ttggagagga taataggcaa
    23401 tcacttcggc ttgcccctga cgaaaatgct ccttctgatt tccctcactc aattgatcat
    23461 ctttctcttt tttgagcgat tcaatctttt gctcaaaact tttttgctgg tagatcttaa
    23521 aagcaataaa tccgcctaat aggcaaatag aaattgctaa aataccaatc aaagccaata
    23581 atacatgtct cgttttcccg tgtctgccac gtcccagtct acttttatcc atatttatat
    23641 cataacaaat ttgagcaata atttcaatga tacaatttgg caatccacaa ttcctctagc
    23701 aagccaaagc atcttacatc aactccaaca tccgtatctg tagagattat gtagttttct
    23761 atctatggaa tttctagtag attgaagcta gaatagtaga cctatacttc taaaacattg
    23821 ttagaaatcg attttactgt cctgatcgat ttgtcctatt ctcatttcat tttactataa
    23881 aactatgaaa agctaggtca tctgagaagt aattactatc gacaattgcg atagcgtata
    23941 ctggtgcgat ttgtttgtag ctctggtggg tatcacctta acgttggcga attttttcta
    24001 ggttttgatt aacctgactg cacagataag cccataggcg attgatgaaa aaattctgat
    24061 gatgaatctg aatttcgatg ataacttgga ttccattatc taactccgcc aagacgtcta
    24121 tactagtata gaaatcctgc actgagtagg gcatggaagg caagacgtga atgttacttc
    24181 cctccaaaat cgtcacattt tttgctggca agtccagcat atcgcggata aattgacaaa
    24241 tcattagttg ggctgatacc cggatgtctg agaatcctcc ttttcctcct tctattatag
    24301 cacattttta aatctaggtt tgctagattc tatgcttcta tctatttatt cggaaaagaa
    24361 agcttttctt cagaagaaag ctaatagctt atttgaaatt ttctcctctc tgttggatag
    24421 tcccgtctat ctatgttata atgaaagaag gatttaaaat cggaaaagga gtatttatgc
    24481 taaaattagg tgtcatcgga actggcgcta tcagccatca tttcatagaa gcagcccata
    24541 ccagtggaga ataccaactg gtcgcaatct attctagaaa actagaaact gcagcaacct
    24601 ttgcttctcg ctatcagaat atccaactat tcgatcaatt agaagtcttc ttcaaatctt
    24661 cttttgattt agtctatatc gccagtccaa actccttaca ttttgctcag gcaaaagctg
    24721 ccttgtctgc tggtaaacat gttattctcg aaaagccagc tgtcactcaa ccacaagaat
    24781 ggtttgattt gattcaaaca gctgaaaaaa ataactgttt tatctttgaa gcagctcgta
    24841 attaccacga gaaagccttt actactatca aaaacttttt agcagataag caagtgttag
    24901 gagcagattt caactatgcc aaatactctt ccaagatgcc tgacttgttg actggacaaa
    24961 caccaaatgt cttttcaaac cgttttgctg gtggagccct catggatttg gggatttatc
    25021 ccctctatgc tgctgttcgt ctttttggaa aagccaatga cgcgacttac catgctcaac
    25081 agcttgacaa tagcattgac ctaaatggag atggtatcct cttctaccca gactatcaag
    25141 ttcacatcaa ggctggaaaa aacatcactt ccaatcttcc ttgcgagatt tatacaacag
    25201 atggaacctt gactctcaac acgattgagc atattcgctc agctattttt accgaccacc
    25261 aaggaaatca agtccagctt cctatccaac aggctcctca tacgatgact gaggaagtcg
    25321 ctgcatttgc acacatgatc cagcaaccag atctgaatct ctaccaaact tggctgtatg
    25381 atgcaggctc tgttcatgag ctactatata ccatgcgcca gactgctggc attagatttg
    25441 aggcagaaaa atgaaaacca aactaccgac tgaatggcaa gaactgagtg accaactcgg
    25501 tttccaagaa ttcaccccca ttcaaactca actatttgag ccccttcttg ctggagaaaa
    25561 cctcctagga gtgagccaaa ccggaactgg taagacccta gcgtatctcc taccaagtct
    25621 tctcagacta caaaagaaaa aagcccaaca actcttgatt ctagcaccaa acacagaact
    25681 tgctggacag atttttgatg tatgtaaaac gtgggcagaa gctatcggat tgactgctca
    25741 actcttcctt tcaggttcaa gtcagaaacg acaaattgaa cgccttaaaa aggggccaga
    25801 aattctgatt ggaactcctg gccgtatctt tgaactcatt aaacttaaaa aaatcaagat
    25861 gatgaatgtg gaaaccatca tcctggatga atttgaccaa ttactagatg attctcagat
    25921 tcactttgta gagaaaatca ctcactacgc acctcgtgac caccaactgg tctacatgag
    25981 tgccacgacc aagtttgacc aagaaaagat tgtgccgaat acacgcacta ttgatctctc
    26041 tgatcaaaaa ttggacaata tccagcattt ctacatgcag gtagaccaac gtcatcgagt
    26101 ggatatgcta cgaaaactgg ctcatgtaga ggatttccgt ggtctagtct tcttcaatag
    26161 cctgtcagac cttgggaatg ccgaggaaaa actacagtat cgtgatatat tggctgtttc
    26221 cctcgctagt gatgttaatg ttaaatttag aaaagtcatc ttagaaaagt ttaaagataa
    26281 ccaactaacc ctgcttcttg caactgacct tttggctcgt ggaattgata tcgatagcct
    26341 agaatgtgtc gtaaactttg atattcctag agatagcgaa acctacatac accgtgctgg
    26401 ccgtactggt cgtatgggca aggaaggtta tgttatcact ctcgtcactc atccagaaga
    26461 aatcaaaaaa cttaaaaagt ttgcaagtat ccgagaaatt gtcctaaaaa accaagaact
    26521 ctatatcaaa taaagcccca ttgctctata cacatccagt gtatggagct tttttataag
    26581 caattcaatt ttatactcat ctgctttcaa aaagcattct agtccatttc cgattaacga
    26641 tggactttat cacctccttc tccagtcctt gtatgacatc ttgaagttga ttcatgacat
    26701 cttccaaaat tcgaaaggct ttattcttaa atccacgttt acgaatctct ttccacactt
    26761 gttcaatggg gttcatctct ggtgtgtatg gaggaataaa tgcaaagcca atattagtcg
    26821 gaatctttaa ggtacttgat ttatgccata tagcattgtc cataacgagt aaaagataat
    26881 catctggata agcttgtgaa agctcttcta aaaaggcgtt catccactca gtattacatc
    26941 caccagctat taagaaaaat gattcgcctg tatgggcatc aacagctcca taacaatagc
    27001 gaaattctcg tatatagtga ctatggacat gtggacctac tcctattgga gaccaacaag
    27061 atcccagttt actgattcta ccgaaaccag cctcatcttg gtacatcaag cgaaccttac
    27121 gaaaacgtct actgatttta aagcgctttc ttgtcttctt gaattgagat tttattttta
    27181 gacgcgacaa tggtttgagc gtctgctttc ttaggatgtt ctggacgtgg cataatattt
    27241 cgccaaccat ggcgcttcaa cagttgatag aaggcatcac gtgtgtagga acgacctaac
    27301 tcctttttat aagtctgaaa taaggcatca attgtaacaa attctcctgc ctctgcagcc
    27361 ttcaaatggc gggcaagaaa gactttttct tcctcaactg tcatatatgc atggttacga
    27421 ccaccacgtg tttcttgaag gagagagtcg agtccgaact cctcatattt ttttacattt
    27481 tgctaaatcg ttgt
//
LOCUS       DAVKJD010000018        25141 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKJD010000018 DAVKJD010000000
VERSION     DAVKJD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25141
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.018"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(34..231)
                     /gene="blpI"
                     /locus_tag="VW157_000848"
     CDS             complement(34..231)
                     /gene="blpI"
                     /locus_tag="VW157_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEU9552175.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            513..1100
                     /locus_tag="VW157_000849"
     CDS             513..1100
                     /locus_tag="VW157_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU9552176.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            1063..2670
                     /locus_tag="VW157_000850"
     CDS             1063..2670
                     /locus_tag="VW157_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9552177.1"
                     /translation="MGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYILQQVMSFSR
                     DYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSIIDALASTIL
                     SLFLDVSILILVGGVLLAQNPNLFLLSLLSIPIYMFIIFSFMKPFEKMNHDVMQSNSM
                     VSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQTSLKQGTKL
                     VLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQTKLQSAKVANNRL
                     NEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIKQGDKVSLV
                     GVSGSGKTTLAKMIVNFFKPYKGHISINHQDIKNIDKKVLRRHINYLPQQAYIFNGSI
                     LENLTLGGNHMISQEDILKAYELAEIRQDIERMPMGYQTQLSDGAGLSGGQKQRIALA
                     RALLTKSPVLILDEATSGLDVLTEKKVIDNLISLTDKTILFVAHRLSIAERTNRVIVL
                     DQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            2681..4042
                     /locus_tag="VW157_000851"
                     /pseudo
     CDS             2681..4042
                     /locus_tag="VW157_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
     gene            4099..4254
                     /gene="blpC"
                     /locus_tag="VW157_000852"
     CDS             4099..4254
                     /gene="blpC"
                     /locus_tag="VW157_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9552178.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGWWEELLHETILSKFKITKA
                     LELPIQL"
     gene            complement(4296..5636)
                     /locus_tag="VW157_000853"
     CDS             complement(4296..5636)
                     /locus_tag="VW157_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9552179.1"
                     /translation="MNIAWILLYALVINGLEIVIFFKVDGIGLTFDRIFKAFLLKFLL
                     GIIFTTFQFLAVSKYLSYFIEPLFGIGLSFLLLRGLPKKILIFYGLFPMILVELFYRG
                     VSYFVLPFLGQGIVDGDGNPIFLLIMIFVCFIVLVFLKWLDYDFTRLRREFLDTGFQK
                     SLTKINWAMGAYYLVMQSLSYLEYEQGIQSTTVRHLILVFYLLFFMGGIKKLDTYLKE
                     KLQEELNQEQTLRYRDMERYSRHIEELYKEIRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLRDSSQKLQDNKYDLGRLVNIRDRALKSLLAGKFIKAREKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASAEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTNLNTTCQNQVFRQVLTVIHAE"
     gene            complement(5650..6387)
                     /locus_tag="VW157_000854"
     CDS             complement(5650..6387)
                     /locus_tag="VW157_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9552180.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRPHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(6392..6730)
                     /locus_tag="VW157_000855"
     CDS             complement(6392..6730)
                     /locus_tag="VW157_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9552181.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            7006..7335
                     /locus_tag="VW157_000856"
     CDS             7006..7335
                     /locus_tag="VW157_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9552182.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7503..8552)
                     /locus_tag="VW157_000857"
     CDS             complement(7503..8552)
                     /locus_tag="VW157_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552183.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8549..9280)
                     /locus_tag="VW157_000858"
     CDS             complement(8549..9280)
                     /locus_tag="VW157_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552184.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVADPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9348..9758
                     /locus_tag="VW157_000859"
     CDS             9348..9758
                     /locus_tag="VW157_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9552185.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            9768..10055
                     /locus_tag="VW157_000860"
     CDS             9768..10055
                     /locus_tag="VW157_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552186.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(10745..11881)
                     /gene="dnaJ"
                     /locus_tag="VW157_000861"
     CDS             complement(10745..11881)
                     /gene="dnaJ"
                     /locus_tag="VW157_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9552187.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(12283..12639)
                     /locus_tag="VW157_000862"
     CDS             complement(12283..12639)
                     /locus_tag="VW157_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU9552188.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(12641..14464)
                     /gene="dnaK"
                     /locus_tag="VW157_000863"
     CDS             complement(12641..14464)
                     /gene="dnaK"
                     /locus_tag="VW157_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9552189.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(14944..15468)
                     /gene="grpE"
                     /locus_tag="VW157_000864"
     CDS             complement(14944..15468)
                     /gene="grpE"
                     /locus_tag="VW157_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9552190.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15495..16529)
                     /gene="hrcA"
                     /locus_tag="VW157_000865"
     CDS             complement(15495..16529)
                     /gene="hrcA"
                     /locus_tag="VW157_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9552191.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16693..17202)
                     /locus_tag="VW157_000866"
     CDS             complement(16693..17202)
                     /locus_tag="VW157_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9552192.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCIATFLGGLVVCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            17810..20143
                     /locus_tag="VW157_000867"
     CDS             17810..20143
                     /locus_tag="VW157_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9552193.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYSRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            20156..21619
                     /locus_tag="VW157_000868"
     CDS             20156..21619
                     /locus_tag="VW157_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9552194.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            21619..23169
                     /locus_tag="VW157_000869"
     CDS             21619..23169
                     /locus_tag="VW157_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9552195.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQ
                     NKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
                     NIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDY
                     NFNLLLIALPPLSEQQRIVEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYA
                     MQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVP
                     CEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPET
                     VHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYAWAVADSHVTVIRVLSGVIN
                     CHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQF
                     FAHIDALI"
     gene            23226..24023
                     /gene="psrA"
                     /locus_tag="VW157_000870"
     CDS             23226..24023
                     /gene="psrA"
                     /locus_tag="VW157_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU9552196.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            24034..24642
                     /locus_tag="VW157_000871"
     CDS             24034..24642
                     /locus_tag="VW157_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9552197.1"
                     /translation="MVKLTTCYFCDYGSYYGNKDETTSYPIYEIPEAWRYIKFASLVN
                     FRIGKTPPRSEATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTL
                     LMSFKLSIGKVAILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAI
                     KGKTLNSTSISELLIPISNHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(24590..25135)
                     /locus_tag="VW157_000872"
     CDS             complement(24590..25135)
                     /locus_tag="VW157_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9552198.1"
                     /translation="MNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT         8138 a         4942 c         4565 g         7496 t
ORIGIN      
        1 gcgataataa tactatattt aagaatcata atcttacaaa gaggacataa ttcctgaacc
       61 tacacaaata agtgttgctg ctcccccagt tatcggacca gtcgcagcag ctaatagtac
      121 tgctccaata caaccaccga ttgcagatcc taaattgcct cttcctccac taactatttc
      181 gagttcttca ttatccataa cagaaaattg ttccatcatt tttgtattca tgacaaatac
      241 tccttttttc tttttttatt tttgtcttgt tgtaactttg ataagtttag tatatcatcg
      301 ttttttaaaa tttttcatcc agatcttgaa ttgtcatcga aacgtcttga attaggtttt
      361 ttatttcaag ccacctctaa atgtttaaaa aaaataattt ctaatcactt ttttaccatt
      421 caggaagttt taatgactat tcaagatttc ataaaatatg aacttagttt tatgacataa
      481 tagacctatc cactatatga aaggaattgc caatgacttc ttataaacgt acatttgttc
      541 ctcaaataga tgcgagagac tgtggtgtcg ctgccttagc ctcgattgct aaattctatg
      601 gttcagattt ttctctagct cacttgagag aacttgcaaa gaccaataaa gaagggacga
      661 ctgctcttgg cattgtaaaa gccgctgatg aaatgggctt tgaaacaaga cctgttcaag
      721 cagataaaac gctctttgac atgagtgatg tcccctatcc atttatcgtt cacgttaaca
      781 aagaaggaaa actccaacat tactatgttg tctatcaaac aaagaaagac tatctgatta
      841 ttggtgatcc tgacccttct gtaaaaatca ctaaaatgtc aaaagaacgc tttttctatg
      901 aatggactgg agtagctatt tttctagcta ccaaacccag ctatcaaccc cataaagata
      961 aaaagaatgg tctactaagc aaacttcctt cctctgattt tcaaacaaaa atctctcatt
     1021 gcttacattg ttctctcaag cttattggtc actattatca atataggtgg ttcttactat
     1081 ctccaaggaa tcttggatga gtacattcca aatcagatga aatcaacttt aggaatcatc
     1141 tcagttggtc tggttatcac ctatatcctc caacaagtca tgagcttctc cagagattat
     1201 ctcctaaccg ttctgagcca gagattaagc attgatgtga ttttatccta tattcgccat
     1261 atttttgaac ttcccatgtc tttctttgcg acacgtcgta caggagaaat catttcacgg
     1321 ttcacagatg ctaactctat tatagatgcc ttggcttcta ccattctttc tctttttctg
     1381 gatgtttcta ttctgattct tgtaggggga gtcttgctgg cacaaaaccc taatctcttc
     1441 ctcctttctc ttctttccat tcctatatac atgttcatca tcttttcttt tatgaaacct
     1501 tttgaaaaaa tgaaccatga tgtcatgcaa agtaattcta tggttagctc tgccattatc
     1561 gaagatatca acgggattga aactataaag tcgctcacga gtgaagaaaa tcgctatcaa
     1621 aatatagaca gcgaatttgt agattatttg gaaaaatcct ttaagctcag taaatattct
     1681 attttacaaa cgagtttaaa gcagggaaca aaattagttc tgaatatcct tatcctatgg
     1741 tttggcgctc aattagtcat gtcgagtaaa atttctatcg gtcagctgat tacctttaac
     1801 acactttttt cttactttac aactcctatg gaaaatatta tcaacctcca aaccaaactc
     1861 caatctgcga aggtcgctaa taaccgtttg aacgaagtct atctagtcga atctgaattt
     1921 caagttcaag aaaaccctgt tcattcacat tttttgatgg gcgatattga atttgatgac
     1981 ctttcttata agtatggttt tggacgagat accttaacag atattaatct cacgattaaa
     2041 caaggagata aggttagcct agttggagtt agtggttctg gtaaaacaac tttagccaaa
     2101 atgattgtca atttctttaa accctacaaa gggcatattt ccatcaatca tcaggatatt
     2161 aaaaacattg ataaaaaagt cttgcgccgt catattaatt acctacccca acaagcctat
     2221 atctttaatg gctctatttt ggaaaactta accctgggcg gtaatcatat gattagccaa
     2281 gaagatattc taaaagctta tgaattagct gaaattcgtc aagacattga aagaatgcct
     2341 atgggctatc aaactcagct ctctgatgga gctggtctat caggaggaca aaagcaacga
     2401 atcgctctcg ctcgtgctct tttaactaaa tctcctgttt taatactaga cgaagctact
     2461 agcggtcttg atgtcttgac tgagaaaaag gttatagata atcttatatc cctaactgat
     2521 aaaaccattc tctttgtagc ccatcgtctc agtatagccg aacgaactaa ccgtgtcatt
     2581 gttcttgacc aggggaaaat cattgaagtt ggtagtcacc aagagttaat gcaggcgcaa
     2641 ggcttctacc atcatctatt caataaataa ggagaatgtc atgaatccta atctttttag
     2701 aagcgtcgag ttttatcaga gacgttacca taactatgcg acagtattaa tcatacctct
     2761 gtcattactg tttactttca tcttgatttt ctcccttgtt gccacaaaag aaattactgt
     2821 tacttcccaa ggggaaatcg cccctacaag tgtcattgca tctattcagt cgaccagtga
     2881 taatcctatc cttgctaatc atttagtggc aaatcaagta gttgagaaag gtgacttact
     2941 catcaaatac tctgaaacaa tggaagagag tcagaaaact gccttagaaa ctcaattaca
     3001 aagatttgag aagtaaaaag aaggacttgg aattttgaaa caaagcttag aaaaagcgac
     3061 tgatcttttt tctggcgagg atgaatttgg ctaccataat acctttatga attttactaa
     3121 acaatcccat gatattgaac tgggtatcac aaagactaac actgaggttt caaatcaagc
     3181 taatcttgcc aatagcagtt catcagctat tgaacaagaa attacaaaag ttcaacaaca
     3241 aattggagaa tatcaagagc tgagagatgc tatcataaat aacagagcac gcttaccaac
     3301 tagcaatcca caccagtcaa ttttgaatcg ttatcttgta gcctcacaag gacaaacaca
     3361 aggaacggca gaggagccat ttttatctca aattaatcaa agtattgcag gtcttgaatc
     3421 atctatcgca agcctcaaaa ttcagcaagc tggtatcgga agtgtagcaa cttatgataa
     3481 tagtttagca gccaaaattg aagtactccg cactcaattt ttacaaacag cttctcagca
     3541 acaactaacc gtggagaacc aattaacaga attaaaagta caactagatc aagcgacaca
     3601 gcgcttagaa aacaatacct taaccgctcc aagtaaaggt atcgttcatc tgaacagcga
     3661 atttgaaggt aaaaatagaa ttccaactgg tacagaaatt gctcaaatat tccctatcat
     3721 cacagataca agagaagtac taatcactta ctacgtagct tctgactatc tacctctact
     3781 agataaagga caaactgtaa gattaaaact ggagaagatt ggaaatcacg gcattaccat
     3841 catcggccaa cttcagacaa ttgatcaaac tcctaccaga acagagcaag gcaatctctt
     3901 taaattaacc gctcttgcaa aactatctaa tgaggatagt aaactcatcc aatatggctt
     3961 acaaggtcgc gtcactagtg taactgcaaa gaaaacatat tttgattatt tcaaagataa
     4021 aattttaacc cattctgatt aattttcaga taacactcta taactattta ttatcttatc
     4081 aaaaaggaga atcataacat ggataagaaa caaaacctaa cttcatttca agaactaaca
     4141 actaccgaac tcaaccaaat tacaggtgga ggatggtggg aagaactctt acatgaaaca
     4201 attttaagta aatttaaaat cacaaaagca cttgaactac ctattcagct ataaaaacaa
     4261 gaccgagaaa caagaactct cggtcttgtt ttttatcatt ctgcatgtat cacagtaagt
     4321 acctgacgaa agacttgatt ttggcaggta gtatttagat tggtattggg atgactttcc
     4381 acaattttca taacggtata gagaccaact cctctctcct cccctttaga acttgctcca
     4441 aaggagaaga tttcagaaat atcgatgccc tcttctttga tggagttttc aatgataaaa
     4501 gtctcctgtg ctccattttt taaaaaggcg attgaaacat gaggttgact ggcctctgca
     4561 ctagcttcaa tagcattgtc acaaaggata gacacaatgg ttagaaaatc aagtaggctc
     4621 atcccctcaa cctgaatctc ctcaggaact tcaacattaa agacaatgtt cttttctcta
     4681 gcttttataa attttccagc taggagactc ttgagggcac ggtcacgaat attcaccaat
     4741 ctgcccaggt catatttatt gtcctgcaat ttctgactgg aatcccttaa gaccgagtcg
     4801 tagatctctt ttatctgctc catatcctcc tcttcaatgc ccaaacgtaa gctggttaag
     4861 aggttagtgt agtcatggcg aaaactccga atttccttgt aaagttcctc tatatgccga
     4921 ctatagcgtt ccatatctct gtagcgcaag gtctgctctt ggttcagttc ctcctgaagt
     4981 ttttccttca aataggtatc caatttcttg atacccccca taaaaaagag taggtaaaac
     5041 actaggatga gatggcgaac agtcgttgat tgaatacctt gttcatattc aaggtaagat
     5101 agactttgca tcactagata ataagccccc attgcccagt taatcttagt aagagacttt
     5161 tgaaaacctg tatctagaaa ctcccttctc aatctagtga aatcatagtc taaccatttc
     5221 aaaaagacta aaactatgaa gcaaacgaat atcataatca ataaaaagat aggattgcca
     5281 tccccatcta caattccttg ccccaaaaat ggaagcacaa aataggaaac acctctgtaa
     5341 aagagctcta ctaatatcat tgggaagaga ccataaaaaa taaggatttt tttaggaagc
     5401 cctcttaaca ataagaaaga tagacctata ccgaacaaag gttctataaa ataggacaaa
     5461 tatttactta cagccaaaaa ttgaaaagtc gtaaaaatga tccctagaag aaatttcaga
     5521 agaaaggcct taaaaattct atcgaaagtg agaccaattc catctacttt aaagaaaatg
     5581 acaatttcta gtccattaat aacaagtgca tacaacaata tccaagcaat gttcataaac
     5641 tctcctagct cagtgtaagt tattaatagc ctcagacact tccctgacct tataacgcgc
     5701 gatcagacag cttccaccat tgggaaagaa aaggagtttt tctttcttat ccaaatgcac
     5761 cacatttgca ggattgatga gaaaagagcg atggcactgc aagagacggg gttcctgctt
     5821 aaaaacctcc tctaaactcg ccgtaaattc tagcctgtcc gtcttggtat agagaataac
     5881 acgatggggt cttggggatg tttcgagata gtaaacctct ttgaaaggat attggaattg
     5941 ggcaaatttt gatttaaagt aaaagcaatc ttccgccaga cttttactat cttgactatt
     6001 ggcatagagg agggctgtct cgatacgaga ttcaaactcc tctgctgaca aggccttatc
     6061 aatgtagtcc aaagcagaca cttggtagcg aaaagacagg ggcataaact ccgagtgagt
     6121 cgtcacaaag acaatcaggg cataaggatc ccgatcccga atctttctag ccacttccag
     6181 tcccttcatc tcttcatttc gaatctcaat atccaaaaag aatagctgat gggccccctt
     6241 ctcatgcact tcagccagca gttggtccgg cttgccaaat acctcaaaag agctaggaat
     6301 gatatgatgt gctttcaaaa gtttctcaat cgtcgtttca attctagtct gttgggaaaa
     6361 atcatcctct aaaacaaata ttctcatctt cttactctcc ctctttcaac catttttgac
     6421 gaatttctct atagcgacgt ctggcatact tgacagcgta ttgaccttct tctccgagaa
     6481 aaaggattct ttcttgaaaa tcaatcgatt ttaatttatc aagattaacc aaacaatttc
     6541 gatgacatct catcaaggtt tccccacatt ggttctcaag attacttaaa ttttgaagca
     6601 tattaaaact agcttcttct gtaacaatct gtacggtatg ggctttagtt ggatgtgttt
     6661 ggatatagta gatatcgtcg atgtttactt tatagactgt tttctgcgtc tgaataatca
     6721 tatatttcat tggattctcc tcctaagaaa agaataacag aaaaggtcta gcccaagcac
     6781 acccaatcct aaacagtctt tgaaaactcc taaaaggatt attttctaca acatcatgat
     6841 gagcttacaa ctattttatc ataaaattca ctcagcgcaa ttcaagacat ttcaatgaca
     6901 attaaagatt tgaataaaaa aaaagaatta aaagtgataa actaacagta tcaaaacaca
     6961 gttgccagaa gtaagactgg cacccagatt aaaggaggca ttcccatgac agatacagac
     7021 cccatcaaaa gagctcatac tttgattact gacttaaaca aagcctacca agcatgcaaa
     7081 caggcaagcg ctgacgacgt ccgctttcag gagcaattaa actctattct tggttttcta
     7141 gccaaggctg aaacagtgga taatcgattc ttgattgaac tagaaaaatt ttaccagact
     7201 tccagtcttc tcatgggact cagcgctctt gatccagatg ctccaactcg cgccgcttgg
     7261 cgggcctatg accgcttcca ctttgaccaa gtcaagacca agttaatact caatgaaaat
     7321 caaagagcaa actaggaagc tagccgcaag ctgtacttga gtacggtaag acgacgctga
     7381 cgtggtttga agagattttc gaagagtata agtctctacg gaccaaccat tatcctgtag
     7441 taacaaaaaa gaagttgaga cagactgaac tcaacttctt ttttagtgtc gtatcagcaa
     7501 tgttagtcct gcatctgacg ttttacctga taaggcaaat acaagactag taaaaccaaa
     7561 ccagctccta gtaaggctag aagggctagt ttttcttgga aggtaatcaa cccaacaact
     7621 aattccacaa gtaaaacaaa actggtcaat cctcgaacta cctcctgtaa gcctttttcc
     7681 ttttgcccct tgtccagcgg aaagagttgg gtcaaatact ggtagtcaaa ggcatgatag
     7741 agggccagca actggaagag caagaggtag ttaaagagaa ctaccactgc tgtcgcaatc
     7801 caagcttgct cgataaaaac ctgcgccagt acggaaagta agagaagacg aagactgaga
     7861 gcaaagaggt cgccatttcg cagataagaa cgcagataga gattttgcca aatcttccca
     7921 ggcaccttct gaacagcctt taagataaag tccagatagg cacgacgctt aacgctgttt
     7981 gaaattccct tgacctgcgt aaagagggca aagaaacgaa gcaagacttg cttacgcttg
     8041 ctttcttggg aaatgacata gtcccaatcc agtccagttt cagtgaaaaa tttgctggcc
     8101 ttttgacgaa agtggaaata ttttcctacc cccaataaaa gcacatagag cagaaaaact
     8161 ggcaagccat aacccattgc taaaaataaa ggcgcaaata acagcaagaa aagggtctgt
     8221 acaaagagcc aaaagactag ggaaatgcca gtttgacgct tgagatggag cttgatttcc
     8281 tcttctccaa ctaagagaaa gagcttgtct ggagcctcca tataggtggc aattcctccc
     8341 caaagtaaaa gtaaaacaga cgtaattcct acaaacaaaa ggataggcca atgattttca
     8401 ggaaaatgtt gtaagagttg actgtactgg taggctagaa agcccaacag gacaagcagg
     8461 aacaagacaa agtggtcatt gagcacatag cgcagataac caagacactc cttacgaaag
     8521 gcctgctttc tctttaaaaa caagtctttc ataggtcctc ctcttttgtc agagccaagt
     8581 aaatatcatt caaactagcc tcaggcatat caaaggcttc gcgtagttgc aggagattgc
     8641 ctttggcacg cacctctccc ttgtgaagaa tgacaaaggc atcacacatc ttctccgccg
     8701 aatccagcac gtgggtactc atgagaatag acttgccctt ttgcttctcc acttccaaaa
     8761 gctgaatcaa atcagaaata gccagcggat caagaccaag gaaaggctca tccacgatga
     8821 aaagacttgg atccgccaca aaagcacaga taatcatgac cttctgcttc atcccttttg
     8881 aaaaatgaac agggaaccag tctaatttct gttccaaacg gaacattttt aacaagggct
     8941 ctactcgttc gaaagccact ttttgctcaa taccgtaagc catagcaacc gtttcgatat
     9001 gctctctgag ggtcaattcc tcatacagac taggcgtctc aggaatgtag ccaatctgct
     9061 tgcggtagct agtcgcatct ccttgcagag tcaggccatt gatattgatg gagccactat
     9121 aaggtgccaa cagaccgata atctcattga tcgtcgttga tttcccagca ccattgagac
     9181 caatcaaacc gaccaactgc ccactttcaa cagtaaagga cacatctttc aaaacaggaa
     9241 catgaacata gccacctgtc aggtttttaa tttctaacat attttctcca aatctggtat
     9301 aatgtagcta tattatatca aaattcagta cagtagaggt agattttatg tcagattgca
     9361 ttttttgtaa aatcatcgca ggggaaattc ctgcttcaaa agtatatgaa gatgagcagg
     9421 tccttgcctt tcttgatatc tctcaagtaa cactaggaca caccttggtc gtgccaaaag
     9481 aacactatcg caatcttttg gagatggatg ctacgagcgc cagccaactc tttgcccaag
     9541 taccaaaagt agctcaaaaa gtcatgaaag tcactaaggc tgctggtatg aatatcattt
     9601 ccaactgtga agaagtcgct ggtcaaacag tttttcatac tcacgttcac cttgtgcctc
     9661 gctacagtgc tgacgatgac ctcaagattg attttatcgc ccacgaacca gactttgaca
     9721 aacttgctca agtcgctgaa accatcaaaa acgcttaagg agttgctatg aaattatcca
     9781 acctactgct atttgcagga gctgcagccg gaagttatct ggttacaaaa aatcgccaaa
     9841 ccatcacaga tgaagtcttg aataccactg accgcgttca agctatcaag gacgatgtgg
     9901 atattatcca aaacagcctg caaatcatta accagcaaaa agaacttatc aaggaatacc
     9961 aagaagactt gacttacaag tttaaggtct tggaaaagga tatccagact agactagctg
    10021 tgataaaaga aatgcaggga actgaagata agtaaaaaga gcccgacggc tcttttttaa
    10081 aacgtaaaca agttatagtg aattgaattt agaatagcac atcatagttt ctaaagcatt
    10141 gttagagatt actttaaaat cctcttatca atttgttcat attctatttc aatctactat
    10201 atatatccca tcaactatgc atcataattt aggtaactca tactcaataa aaatcaaaaa
    10261 gcaaactaga aagctaggca cagactgctc aaaacaccgt tttaaggttg tggatagaac
    10321 tgacgaagtc agtaaccata cccatgacaa gccgacgctg acgtggtttg aagaaatttt
    10381 tgaagagtat cattcaccat ttcacaacta aatcgaagac tttctgccgt aatgaaaaca
    10441 cctgaaacag cttggtttcg gctatccgga aactttaaga cttaggttca aagtttaggt
    10501 atggaacttc gaaggaggtc gctaccgtcc gtcattactt aggaaagtct taaaaaatct
    10561 ataaagtaaa aagagcccga cagctctttt tgtttatact caatgaaaat caaaaagcaa
    10621 actaggaaac tagccacggg ctgctcaaaa cactgttttg aggttgtaga tacgactgac
    10681 gaagtcagtc acatatatac ggcaaggcaa cgctgacgtg gtttgaagag attttcgaag
    10741 agtattattc tccatcaaag gcatctttaa tatggtcaaa gaagcctttt ttctttggat
    10801 ttactttcaa gtcaccagca gccgcgaatt ctttcaaggc tactttttgg cggtcgttca
    10861 agcctgtcgg tgttacgaca ttaacagtaa cgtattggtc accaactgca ccgccacgaa
    10921 ggctcggtgc ccccttacta cgtaggcgga acttcttacc agtctgagtt ccctctggaa
    10981 taaccaattc aacatcaccg tgaacagttg gaatatctac tgtatcacca agagccgctt
    11041 ggacaaagtt gaggttgaga ttgtagaaga tagtcgttcc ttcacgttca aacttgtcgc
    11101 tagcttccac agaaactact acatacaagt caccataagg tccaccgtta aagcctgctt
    11161 caccttgacc agcgaggcga atttgttgac ctgtttccac accagcaggg attttcacat
    11221 gtacgctatg agcttgtttc tcatgacctg ttccatgaca ggttgtacat ggatatttga
    11281 tttcttttcc tcgaccgtga cagacatcac aggttacttg gcgacgcatc ataccaagag
    11341 gagtctgcgt atcgacgtta atgacaccag cgccatgaca gcgtccacaa gtgactggac
    11401 ttgtccctgg cttagcacca gatccattac atgtacgaca gccagcttca cgatgatact
    11461 taacttcctt ctcagttccg aagatagctt cttcaaaggt caaattgaca cgatactgga
    11521 gatcatctcc ttggcgagga gcgtttggat tgcgcgaaga accgcctccg ccgaagaaac
    11581 ttgagaaaat atcctcaaaa ccaccgaagc cacctgcccc attgaaaccg ccgaaaccac
    11641 cagctccacc aaaaccacca ttggcgcctg cagcaccata ctggtcatag gcagcacgtt
    11701 tttggtcgtc actcaaagtc tcataggctt cttgaacttc cttgtacttg tcctcagcac
    11761 caggctcctt gttgatatct gggtgatatt ttttggaaag cttacgataa gcctttttga
    11821 tttcgtctgc cgaagcgttt ttggataccc ccagacgatc ataaaattca gtattgttca
    11881 taattcaaga tacaaagggc gtcaaacgga cacagccatc taggagtttt gacgacggac
    11941 gcttacgtcc tagaaaaaac tatctttttg gcacagtcca tagcccgtgt tcagttacga
    12001 acacccttat tcctttctat tgatgttctg aatcgaacca ccatttaggt cgggttcaat
    12061 tcctttcttt tatatttagt aagaaattct gaaacccgtg ttcagttacg aacactcttt
    12121 gttcctttct attgatgaga caaaacccaa acaaatgaga ttccgtccct ttttatcgaa
    12181 aaacagaggc tgggttgcaa cctctgcaaa taagtaaatt aactttattt tgattttaaa
    12241 tatcctataa gtacagaaat atttatatat ttttgtctct tcttagtatt tgcctatata
    12301 attccaattg atcataagaa atacaactac tgtccaaatc ataccaatta tgaacggtat
    12361 tacaaattta tatgtttgta tttctaatga tatcatagct gataccatcg ctgatacaaa
    12421 tgcagacaac aatactgaag taacaaaatt ccttaccgct tcatcatttt tgacacaact
    12481 atcatgctca gctaatataa tttctctata tatagtcaaa cattctactg gaatattatc
    12541 tataaaacct tttgctgctt catagttgtc agcttgatta caaaaatcat tataaacttt
    12601 tcgtgtattt tttagatact catcatccaa tacactcatc ttatttttcc gtaaactctc
    12661 cgtctacgac gtcatcgcct gcgtttcctg ttgcttgtgc gccttctgct ccttcttgag
    12721 cttgttgcgc tgctgcggct tgttcgtaga gtttcacagc aaggccttga gctttttcgt
    12781 tcaacgcttc aagtttcgct ttcatgtcgt ccaagttgtt gtcttcttga gctttcttaa
    12841 ggtcatcaag ggcagcttgg gcagcgtcac gttctgcgtc gaagccttta ccttcagttt
    12901 ccttgattgt cttttcagtc gcaaagattg cttggtctac ttcattacga aggtctactt
    12961 cttctttacg tttcttatcg gcttcagcgt ttgcttctgc atctttcatc atgcggtcga
    13021 tttcttcgtc agtcaaacct gagttcgatt ggatgacaat agtttgttct ttttgagttc
    13081 caaggtcttt ggccttaaca gacacgatac cgttcttgtc gatgtcaaat gttacttcga
    13141 tttgaggaat tccacgaggt gcagctggga tatcagtcaa ttggaagcgt ccaagagtct
    13201 tgttatctgc tgccattggg cgttcacctt gaagaacgtg gatatcaacg gctggttggt
    13261 tgtctgctgc tgttgagaag acttgtgatt tagatgttgg gattgtagtg ttgcgatcga
    13321 taagttttgt aaatactcca cccattgttt cgataccaag tgacaatggc gttacatcaa
    13381 gaaggacaac gtccttgaca tcaccagtaa tcacaccacc ttggatagcc gcacccatag
    13441 caactacttc atcagggttt actgatttgt ttggttcttt accagtttca gctttaacag
    13501 cttcaacaac ggcagggata cgagttgaac caccaacaag gataacttcg tcgatttctg
    13561 acaagctcaa acctgcatct gaaagggctt gacgaactgg aacttttgta cgttcaacaa
    13621 ggtcacgagt caaatcatca aatttcgcac gagtcaaagt catttccaag tgaagaggtc
    13681 cagcctcacc tgcagtgata aatggcaagc tgatttgtgt tgaagttaca ccagaaaggt
    13741 ctttcttcgc tttttcagcc gcatctttca aacgttgcat tgccatcttg tcagtagaca
    13801 agtcgatacc gttttctttc ttgaattctg ctaccaagtg gtcaatgatt ttttggtcaa
    13861 agtcgtcacc accaagtttg ttgtcccctg cagttgacaa tacgtcgaag acaccgtcac
    13921 ccaattcaag gatagagacg tcgaatgtac caccaccaag gtcaaatacc aagatttttt
    13981 cttctttgtc agtcttgtcc aaaccataag caagagctgc tgcagttggt tcgttaacaa
    14041 tacgttctac ttcaagacca gcaattttac cagcatcttt tgttgcttga cgttgagcgt
    14101 cgttgaagta agccggaact gtgataacag ctttggttac tttctcacca aggtagtctt
    14161 cagcgtagcc tttcaagtat tgaaggatca tagctgagat ttcttgtgga gtgtattctt
    14221 ttccatttgc agaaactttt tcagaagttc ccatcttaga tttgatagag ataactgtat
    14281 ctgggtttgt gactgcttga cgttttgcag catcaccaac gatgatttct ccgtttttga
    14341 atgagactac agatggagtt gtgcggtttc cttctgggtt tgcgatgatt ttgctttcag
    14401 ttccttcaag aactgcaact gctgagtttg ttgtacctaa gtcaataccg ataattttag
    14461 acatgtgttt ttctccttgt tagttaattt tctatttttc tttttgatac tcttcttaag
    14521 tggcgacgcc gtcagagctt gacttcgtca atctctttga ctaaactttg agcctaaggt
    14581 ctcaaaattt gcgcaaatag cgccacagcg aagagtatcg tctttggttc gcttcgctca
    14641 ctattacaaa gccaaacata ttttatcttt cttcatagtt tattgtcgtt tcggacaagt
    14701 tttctattat gtaaattgcg acacgaggag tcgaaatcga ttttatttcg acgacgagtt
    14761 agtaaggaag ctagacaaac gccatagcga ttgccgtttt ctgacgagtt gctttagcta
    14821 ccgtcagaat tgccgaatcg aacacgccct aagctctgag taaaaaagat agattttctt
    14881 gtgttcatcg aacacttcgt caatctccta tttttactgc gagcttttta cgggctttgt
    14941 atcttagtta tacaccacta ccattgctgg gcgtaggatg cggtcatgga gtttgtagcc
    15001 tttttgaaag acttgggcga tggtatctac tgggtgttca tcgtctgctg ggagagtttg
    15061 gatggccata tggtagttat ggtcaaattc gccatctgct gcgatttctt caattccttc
    15121 ttctttcaaa gcgtgaatca agctttcttg caccatcccc aagcccttct tcacatcatc
    15181 tgtcaaacct tcaactgcaa gtgcacgctc aaggttgtca agagatggta agattgcttt
    15241 tgccaagtcc tggctacgat aacgttgcaa gttttgacgt tcttcattgg cacggcgttg
    15301 gatattttgc atttctgcat gagcgcgaag atatttgttt tcgaactcat ctgcacgttc
    15361 atttgccaag tccaactcag acttttcagg agttgtttct tcagctgttt ccacaacttc
    15421 ctcttcttga acttcttcta cttcttcatt ttttatatct tgggccatgt tcgcctcctt
    15481 taatgatttc aatcttaatg tacttcgtaa tgattactgc taaggtagcg gtaaaaatct
    15541 gtcaacttca tggtcaaaac acggttgacc acattgactt gattgattag ctgttggtaa
    15601 tccagattaa ctggaccgat aatggctaaa attccaactc cccgataagg aatgaggaac
    15661 ttactactaa tcaccgctag gtcagctaaa caggactctt gaccgtctgc aacacgaaca
    15721 ttttgcatct gatcctcacg caacccctca cgaatctcca aggccacctt ttgcggttgg
    15781 tcaaagaact gataggctgc tagattggca aaattcaaga gatggacctt gcccgccatc
    15841 acaatgtttt cgttgaacat ttccttaaag atgtgttcaa agagatcgat gacattatcc
    15901 gttgttgtaa agtaacgctg gataatctgc ggaatctccg tccgaatctt gtagtgaata
    15961 tctaaaacgg tgtgaccgag gaaacgttcc tgaatgatgc tcttcagttt cagcaaatcc
    16021 tcctgcaaga agttccttgg aatcagaaac tgactagtaa ccgttcgcga ctcgtctagg
    16081 gtaaataccg ccaaggctgt atgttgcccc aaaacaacga tatcaaaggc tgtcaaacgt
    16141 tgcctgctcg gctcaacatc cagtgccact accgtacagc cactcaggtc tgttagcaag
    16201 ttagcagcct cttgcagaat atcctccaat ttgaagaatt cctgatcaaa ggctttgaca
    16261 atctcatata cctcattttc agccagtcgg tcaaaatcca gtgagtgttt cacatagtac
    16321 tgaaaaccag caacacttgg catccgaccg cttgaagtat gagccttctc aagcaaccct
    16381 tgtttttcta gttccgccat gtcattacga atggttgcac tgctagagtt aatagactct
    16441 tgcaaggctt ttgatccgac aggttcgtgc gttttggtaa agatgtcaat aatcagattt
    16501 aaaatatcct gctgacgctc tgtaaccatc tcatcactcc tctctgtctg atcaattagc
    16561 actcaatgca tccaagtgct aacttatgat tctattatac acccttagaa gagaatgtca
    16621 agacaaaaac tcaaaaaatt agcactcttt tataaagagt gctaaaaatc aatctgaaga
    16681 cctagaacct tgctattcat ctactcggta tttccttttt agtctgaaat aaccatgaat
    16741 tagataagaa atcagaaaag catataaaac aaaaataaga aagaaagaca tagaaagatc
    16801 ctctctaaac aaactcatac aaacaacaag accacctaag aaagtagcta tacaggaacg
    16861 aagtctagat cgcataactt cccatttgag ccccttactc atatagactt gatcctctgg
    16921 aagtaaatca gaagacgatt tacgaagaat cgaacaagaa cctgctccta tcaattccca
    16981 acctctatct ctaaaatctt gcagttcacg cttatgctct ttaagaaatc tagaatcata
    17041 gatacgatag ataacatcgt ccggttgacc ttgctcaaaa tagaacaaac caaaacgact
    17101 agttctatac ctccaacctt tcaaatgcat ctcatgtaaa tattcttctt tcttgtccaa
    17161 atcaacaatg gtgaaaatcc gaaattctac tctgctattc attgtcttac cccaaaatta
    17221 gaaaacatgc ctagcgttat ttattagata attctttcca cttttgactc aatctccaaa
    17281 aaatataaga aatctgaatc gcaaaaacta tcaataaaac ccaatctatt atgaaaatca
    17341 aaaacacttc ccaactgaaa gaactacctc cagtgacaaa ctttgagaaa aacggtagta
    17401 gagctaaaaa gagaagcaaa ataggaagca tccgcattgt taaaatcctt ttggcataaa
    17461 aaaatcttta tttaaacgaa aatattatgg caaaatttac gccagttttt gaacggctga
    17521 tgtagatatt ttatactttc aaaatgttta aatgtgatta tttatttttg aaaaatagct
    17581 caccagcccg actgaaagtg cttatagaat gataataagt cgcctgccga aaacagcgaa
    17641 aaatagcagt gttatgcgga gataatctga cgcgatgcga aagtatattg catacttatt
    17701 ttcaacaatt tagcagagta tttttataag tgtgatataa tagaagtata atttgttctg
    17761 atagtttatt ttatggagaa gtagattttt agaatgcgga ggattcaata tggttgagtt
    17821 tataaagtct aagaaagaaa tgagtgagga ggatattaaa gcaaatttca tcactcctgc
    17881 tattgtatcc aaaggatgga aaaatggtga gcatatcgct tacgaagaat acttcactga
    17941 tggtcgaatt gaagttagag gagataaggc tcgtcgtaaa gaaggaaaaa aatcagacta
    18001 ttcactgtat taccaatttg gaactcgaat tgcaattgtt gaggcaaagg ataataaaca
    18061 cagcgttcga gcaggattac aacaagctat tgaatatgga gagattttag atgttccatt
    18121 tgtttattct tcgaatggtg atggctttat tgaacacgac cgtatcacga gagaagaacg
    18181 tgagctggag ttagacgaat tccctactcg tgaagaatta ttttctcgta tgacgaagga
    18241 aaaaggattg acgtacgaaa ttacagaagc tatctcaact ccatactata cagacgcctt
    18301 ctcaatgaaa acgccacgct attatcagca aatagctatc aaccgtacta ttgaaacagt
    18361 tgccagagga caaaaacgag taatgtttgt gatggcaaca ggaacgggga aaacgttcat
    18421 ggcttttcaa attattcatc gccttcgaaa agctggtttg gctaaacgag ttttattctt
    18481 agcggataga aacatcttag tagaccaaac gatggctgaa gactttaagc cattcgaaaa
    18541 ggtaatgacg aaaattacac caaaactttt gactgctcct gaaaaattaa attcttttga
    18601 aatttatcta gggctttatc agcaactaac tggtgaagat ggaactgaaa cacattatca
    18661 aaaatttgac aaagacttct ttgatttaat cgtaattgat gaagcgcacc gtggttcagc
    18721 taaggaaaac agtaactggc gtaagataat tgattatttc agttctgcga cacagattgg
    18781 gatgaccgct actcctaaag aaaccaagaa tgcttccaat acggaatact ttggtgagcc
    18841 aatctatact tatagtttaa aacagggaat cgaggatggt tttttggctc catatcgtgt
    18901 tatgagggtt aatttagatg tggatgtgga tggttatcgt ccagaaactg gaaaagttga
    18961 tgctaacgga caattaatag aagataggta ctacagcagg aaagattttg ataaaaccat
    19021 tgtcattgat gacagaacgc aaagagttgc caagtttgtt tctgattata tgaagcaaaa
    19081 caatgcacga tttgataaaa caattgtttt ttgtgttgat attgaccatg ccgagcgaat
    19141 gcgtgctgca cttgtaaaag agaatctaga cttagtccaa gaagactatc gttatgtcat
    19201 gcaagtaact ggtgacaacg ctgaaggaaa agctcaactg gataacttta tggatgtcaa
    19261 ttctaatttt cccgctattg taacaacgtc taaattatta acgacaggag ttaatgctaa
    19321 aacatgtcgt ttgattgttt tagactctaa tatccaatcc atgactgaat ttaaacaaat
    19381 tattggtcgt ggcacacgtc tttatcctca aaaggggaaa gaatttttta cgattattga
    19441 ttttcgaaat gttaccaatt tgtttgctga ccctgatttt gatggtgagc cagtgaaggt
    19501 gctggaaaca ggtgcgaaaa cagtcagtgg ttctacgccc ggtttcgtag atgaggaagg
    19561 tgacccagta gaaaaatata tcgttacaga caagcaggtt accattctta attctactgt
    19621 tcaagtattg gatgaaaacg ggaaactgat taccgaaagc ctgaccgact acactcgaaa
    19681 gaatatctta ggtagctacg ccactttgaa cgattttatc acagtttggc atacggcaga
    19741 taaaaagaag cttatcttag acgaacttta taaaaaagga gtttatctag atgctattcg
    19801 agagtcggag ggaatatcag aacaagaaat cgatgatttt gatttactcc taaaacttgc
    19861 ctatggtcaa aaagaattaa ccaaaacgga acgtatcaat aaactcaaac aaagcggata
    19921 tttatataaa tatagtgagg aagcgcgtgc tgttttggaa attttactga acaaatacat
    19981 ggataaaggt attggagaac tcgaaagcat tgaaacatta aaacttccag aatttcagat
    20041 atatggtgga accttcaaaa tcatcaatac ttattttgga gataaaaaac gatatttaca
    20101 agcaattaaa gaattggagc aagagctatt tacagtagct taatgaaagg aaagtatgtc
    20161 aattacatca tttgtaaaaa gaattcaaga tatcactcga aacgatgctg gtgttaatgg
    20221 tgatgctcaa cgtattgagc aaatgtcttg gttattattc ttaaaaattt atgatagccg
    20281 tgaaatggtt tgggaattag aagaagacga gtatgagtca attatcccag aggaattaaa
    20341 atggcgaaat tgggctcatg ctcaaaatgg ggaacgggta ttgacaggcg atgaattact
    20401 tgattttgtc aataacaagt tattcaaaga gttgaaagag cttgaaataa cttcaaatat
    20461 gcctattcga aaaacgattg ttaaatcagc ttttgaagat gcgaacaact atatgaaaaa
    20521 tggcgtcttg ttacgccaag tcatcaatgt tattgatgaa gttgatttca atagccctga
    20581 agatcgtcat tcgtttaatg atatttacga aaaaattctt aaagatatcc aaaatgctgg
    20641 gaactcagga gaattttata cgccacgtgc agcgactgat tttattgccg aagttcttga
    20701 cccaaaactt ggagaatcaa tggcagacct tgcttgcgga acaggaggct tcttgacttc
    20761 gactctgaac cgtttaagta gtcaacgtaa aactagtgaa gataccaaaa aatataatac
    20821 agctgttttt ggtattgaaa agaaagcatt tcctcatctt ttagcagtta caaatctgtt
    20881 tcttcacgaa attgatgacc ctaaaattgt tcatggaaat actttggaga aaaatgttcg
    20941 tgaatatacg gatgatgaaa aatttgacat tattatgatg aatccacctt ttggagggtc
    21001 agaattagaa acaataaaaa ataactttcc agcagaatta cggagttctg aaacagctga
    21061 tttatttatg gctgtcatta tgtatcgttt gaaagaaaat ggtcgtgttg gagttatttt
    21121 acctgatggt tttctatttg gtgaaggtgt aaaaactcgc ttgaaacaaa aactggtaga
    21181 tgagttcaac ttacatacga ttattaggtt gcctcatagt gtctttgcac cgtatacagg
    21241 aatccatacg aacattcttt tctttgataa aacaaagaaa acagaagaaa cttggtttta
    21301 tcgtttagat atgccagatg gttataaaaa tttctcgaaa actaagccga tgaagtcaga
    21361 acacttcaat cctgttcgtg actggtggga aaatcgtgaa gagattctgg aaggtaagtt
    21421 ctacaaatct aaatcattta cacctagtga attggctgag ttgaattata atttagacca
    21481 gtgtggcttt ccaaaagagg aagaggaaat cttaaatccc tttgagttga ttcagaatta
    21541 tcaagcggaa agagcaactt taaatcataa gattgataat gtattagctg atattttgca
    21601 gttgttggag gacaaataat gacaccagaa caacttaaag caagtattct ccaaagagcg
    21661 atggaaggga aattagtgcc gcaaaatccc aatgacgaac ctgcaagtga attattaaag
    21721 agaattaaag ctgaaaaaga aaaacttatc agtgaaggaa aaatcaaacg agataaaaag
    21781 gaaactgaga tatttcgtgg tgatgatggg aaacattatg ggaagtttgc tgatggaagt
    21841 actcaagaaa ttgatgttcc ttatgatatt cctgatactt gggagtgggt gaggataaaa
    21901 tcaatttatt ggaattttgg gcaaaataag ccagagaaat cctttaggta tatagatacg
    21961 tctagtattg atagaaaaaa gaacataatc aactacaaaa atctacaata tctttcacct
    22021 gaacaagcgc cttcccgtgc cagaaaatta gtttcgcaga atagtgtctt attttcaaca
    22081 gttagaccat atctaaaaaa tattgctgta gttagagaac ttaaagagta tttgatagct
    22141 agtacagcat ttattgtttt ggatacttta cttaacgaaa catatttgaa atattattta
    22201 ttatcagata attttatcaa tcgtgttaat aataaatcta ctggaacaag ttatcctgca
    22261 atcaatgatt ataattttaa tctattatta attgctctcc ccccactatc cgaacaacaa
    22321 cgaatagtag aagcaatcga atcagcttta gaaaaagtag atgaatatgc tgaaagttat
    22381 aatagactag aacagctaga taaagaattt ccagataaac taaaaaaatc tattcttcaa
    22441 tatgctatgc aaggaaaatt agttgaacaa gacccaaatg atgaatcagt cgaagtttta
    22501 cttgaaaaaa tacgagcaga aaaacaaaaa ctctttgaag aaggcaagat taaaaagaaa
    22561 gatttggaca tttctattgt ttcccaagga gatgataact cttattatga ggaagtacct
    22621 tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc ctacattcaa
    22681 agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc tcagaaatgt
    22741 aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga aacagttcat
    22801 agttaccaga aagaaagatt actaagggat ggtgatttaa tgtggaattc aacaggtcta
    22861 ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgc ctgggctgtt
    22921 gcagatagcc atgttacagt tataagagtc ttatcggggg ttattaattg ccatttcatc
    22981 tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc gtcgggtagt
    23041 acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc cctcccaccc
    23101 cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca tattgacgca
    23161 ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa gataggagtt
    23221 ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa aatactgtgc
    23281 gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa atggaagatt
    23341 tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc aatttgcgac
    23401 tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa ttaaaatttg
    23461 tcaaagtaca gcaaaaaccg tttttagaag atgtcattag ccatgctgat tacctttttc
    23521 ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt tggtttttag
    23581 gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat gtagaaatcg
    23641 gttattttga catttactca aaaggtggta aaattcgtag actgtacatt cctaaaaaat
    23701 taagaaatag ttgtcttagt tggttagaat cagaaaatcg tcgaagtggt tatttatttt
    23761 tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta aaaaattatg
    23821 ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg catttatttg
    23881 ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg atgggacacg
    23941 aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa caaaatattg
    24001 tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac ctgctatttt
    24061 tgtgattatg gctcttatta tgggaacaaa gacgaaacaa cctcttatcc tatatatgaa
    24121 atcccagaag catggagata tattaaattt gctagcctag ttaacttcag aataggaaaa
    24181 actcctccac gcagtgaagc tactttttgg ggaactgaaa taccttgggt atctatatca
    24241 gatatgccta tatctggtta tgtaactaat acgagagaat ctatttctaa attagcatta
    24301 aaatcaaaga aaatagacat ttcacctaaa ggaactttat taatgagttt taaattatct
    24361 attggaaaag ttgccatatt ggatattcct gccactcata atgaagccat tatatcgata
    24421 ttcccctatg ctaataaaga aaatattatc agagattact taatgatatt tttgccactt
    24481 atctctactt taggtgattc aaaagatgct atcaaaggga aaacattaaa tagtaccagt
    24541 atctccgaat tattaatccc tatttctaat catgaagaga tgaaaagaat catttccaaa
    24601 gttgatttac tttttcaaaa agtttctcaa ctttttgagt aataagttcc tgttcctcaa
    24661 aaggagctaa cggaattaat agctcgctca gtgtagtttt aggaatatta tataaagctt
    24721 gacctgatag tttagttatt gctttcaatt gtttataaaa taacggagaa gacaagttaa
    24781 ataatagaaa ttttgaaata atctctgaac tttcgaatgg tgttaattgg aaaataaatc
    24841 caccagccac aacaccatca tagtctttat cgattcttgc aaactttcca atatgttcta
    24901 aagaggttga tacaggtgtt attagctgat tatgttttaa ataaacttgt tcagaggaga
    24961 tgaattgtgt atcaatgtag taatcattat ccaacagaga aaattctaaa ggcttaatat
    25021 taccaccacg tataattcta acacctttat tattaatgct taaatcgccc ttcttgtaag
    25081 aaagacctgt attcattgaa aaaatatctt ttatttttat aacaacccaa ttcataggta
    25141 t
//
LOCUS       DAVKJD010000019        24487 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKJD010000019 DAVKJD010000000
VERSION     DAVKJD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24487
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.019"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(98..2356)
                     /locus_tag="VW157_000873"
     CDS             complement(98..2356)
                     /locus_tag="VW157_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9552199.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2585..2815)
                     /locus_tag="VW157_000874"
     CDS             complement(2585..2815)
                     /locus_tag="VW157_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9552200.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3005..3688
                     /locus_tag="VW157_000875"
     CDS             3005..3688
                     /locus_tag="VW157_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9552201.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3688..4422
                     /locus_tag="VW157_000876"
     CDS             3688..4422
                     /locus_tag="VW157_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9552202.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4576..5433
                     /locus_tag="VW157_000877"
     CDS             4576..5433
                     /locus_tag="VW157_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9552203.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5575..6447
                     /locus_tag="VW157_000878"
     CDS             5575..6447
                     /locus_tag="VW157_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9552204.1"
                     /translation="MKVIIQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6729..7412
                     /gene="rpiA"
                     /locus_tag="VW157_000879"
     CDS             6729..7412
                     /gene="rpiA"
                     /locus_tag="VW157_000879"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9552205.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7426..8637
                     /locus_tag="VW157_000880"
     CDS             7426..8637
                     /locus_tag="VW157_000880"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9552206.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAEINPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8639..9181
                     /locus_tag="VW157_000881"
     CDS             8639..9181
                     /locus_tag="VW157_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU9552207.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9197..10006
                     /locus_tag="VW157_000882"
     CDS             9197..10006
                     /locus_tag="VW157_000882"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9552208.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10503..11525
                     /locus_tag="VW157_000883"
     CDS             10503..11525
                     /locus_tag="VW157_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEU9552209.1"
                     /translation="MKESENDAPKLETPLREEPRLAPQTLPEASEVLENKREESKVEI
                     TEPAQDSPILAPVEETKEEAVTEKPTNTRSLTAEDLVKISKGELHLENDLIDESFYGE
                     KALDLEGDDDQDGIKNKDGKDYLGYNSHPLLADSDGDGLADGEDDNKKEWYVTDRDSL
                     LFMELAYRDDDYIEKILYHKNLFSSLYLDRQEHKLMHNELAPFWKMKKAYYTDSGLDA
                     FLFETKSDLPYLKDGTVYMLAIRGTRVNDAKDLSADLVLLGGNKPAQADDIRKVVGEL
                     AKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDFYNHVLRKVTTFSAPKVITSRTVW
                     DAKNGF"
     gene            11535..12110
                     /locus_tag="VW157_000884"
     CDS             11535..12110
                     /locus_tag="VW157_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEU9552210.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPRSFSQPSAEPME
                     NIALGKQVTQSSTVFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            12135..12647
                     /locus_tag="VW157_000885"
     CDS             12135..12647
                     /locus_tag="VW157_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9552211.1"
                     /translation="MSAQITINHKKVRYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIVRYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            13305..14015
                     /gene="deoD"
                     /locus_tag="VW157_000886"
     CDS             13305..14015
                     /gene="deoD"
                     /locus_tag="VW157_000886"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9552212.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(14038..14562)
                     /locus_tag="VW157_000887"
     CDS             complement(14038..14562)
                     /locus_tag="VW157_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9552213.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(14615..14851)
                     /locus_tag="VW157_000888"
     CDS             complement(14615..14851)
                     /locus_tag="VW157_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9552214.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLVK"
     gene            complement(14919..15839)
                     /locus_tag="VW157_000889"
     CDS             complement(14919..15839)
                     /locus_tag="VW157_000889"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9552215.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            16029..16432
                     /locus_tag="VW157_000890"
                     /pseudo
     CDS             16029..16432
                     /locus_tag="VW157_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16648..17229
                     /locus_tag="VW157_000891"
     CDS             16648..17229
                     /locus_tag="VW157_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9552216.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEKNNA
                     KATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            17226..18503
                     /locus_tag="VW157_000892"
     CDS             17226..18503
                     /locus_tag="VW157_000892"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9552217.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVHVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDVLDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            18521..19183
                     /gene="deoC"
                     /locus_tag="VW157_000893"
     CDS             18521..19183
                     /gene="deoC"
                     /locus_tag="VW157_000893"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9552218.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVRLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            19170..19559
                     /locus_tag="VW157_000894"
     CDS             19170..19559
                     /locus_tag="VW157_000894"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9552219.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAVFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19649..20701
                     /locus_tag="VW157_000895"
     CDS             19649..20701
                     /locus_tag="VW157_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9552220.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20845..22380
                     /locus_tag="VW157_000896"
     CDS             20845..22380
                     /locus_tag="VW157_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552221.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEISAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            22373..23431
                     /locus_tag="VW157_000897"
     CDS             22373..23431
                     /locus_tag="VW157_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552222.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLTELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGSVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            23434..24390
                     /locus_tag="VW157_000898"
     CDS             23434..24390
                     /locus_tag="VW157_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552223.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         7141 a         4415 c         5572 g         7359 t
ORIGIN      
        1 atcataatgg acaaagggca agctgtcaag aaattacagt tattttagta aaaaaagaag
       61 cagaaaaatt tcattttcta ctccttttct ataggattta tttttctttt tcagaacttt
      121 taacttcagc ttttttggca gatttaatct gaatgtttcc cttgctagtc ataactgctt
      181 tgagatcttt ttcgcttgga ttttcaaggt agtagtcagt gattgtgtcc tcaatatagt
      241 cttgaatagt ccgacgaagt gggcgtgctc ccatttttgg atcataacct aggtcaacca
      301 acttttcctt gaccttgtca gttacatcca aacgaatgtt gttgctagag aggcgcttgt
      361 taacatctgc tagcatgagc tcgacaatct gaaggaggtt atccttgctg agagccttaa
      421 attcgataat gccatcaaaa cggttcataa actctgggct aaagaagtta ccgagttcac
      481 cgaggacaga attggtacgt ccttctctag cagcaccaaa tccaacgcta gcttcggtct
      541 ttcctgtacc tgcatttgag gtcatgataa tgatggcatc cttgaagcta acggtgcgtc
      601 cttgcccgtc tgtcaaacga ccatcgtcca agacttgaag aaacatgtgc ataacatctg
      661 ggtgagcttt ttccacttca tcgagaagga tgagagaata cggattgtgg cgaacttttt
      721 cagttaattg accagcctca tcatagccaa cataacctgg aggagcgccg accaacttag
      781 ccacactatg tttttccatg tattcactca tatcaaagcg aatcatacta tcagcagaac
      841 caaaaagttc gatagccagt tgtttggaaa gttctgtctt accgacacca gttggtccaa
      901 cgaagaggaa gcttccgatt gggcggttag gggtaccaag tccgacacga ttacggcgaa
      961 tagccttggc aatcttatcg actgcatcat cttggccaat aacatgagac ttgagatctt
     1021 cggctagatg gatgagttga gattgttctt tctctttcaa atcaccaaca gggatattgg
     1081 ttttctgctc gataatgtgc tcaatagttt tctcgctgat gataggagta tcctggtctg
     1141 tgatcttttt cttttgcatt tccttatact tggcaatctg gtcgcggaag taggccgcct
     1201 tctcaaaatc ttcttctcgt gtagcttgag acttgagatt ttcagcctca atcaagcgct
     1261 gatcaattac tttaggatcc acaaaattca aggtcaagtt catcttagaa ccagcttcat
     1321 ctaggaggtc aatggccttg tcaggcaaga agcgatcttg gatgtagcga ttggaaagag
     1381 ttgcagctgc ttcaatcgca gcatcggtat attgaacgtg gtggtaatct tcgtatttct
     1441 tttgaatccc tttgagaata gtgattgttt cgtccaccgt tggttcatcg actttaacag
     1501 gctgcatacg acgctcgagg gcagcatcct tttcaatgat acggtattca ttgagggtag
     1561 tagcaccgac tagttgcagt tctccacgag caagggctgg cttgaggata tttcctgcgt
     1621 ccatattacc atcactcgca gaaccagcac caacaatttc atggatttca tcgataaaga
     1681 ggatgatatc ttcacgtttg cgaatttctt ccatgagttt ttgcatgcgt tcttcaaatt
     1741 gtcctcgaat ccccgttcct tgaactaagc taaccacatc cagacggatg acttgtttac
     1801 cttggagttt atgtggcaca tcgccatcga caattttctg agctagacct tcgacaacgg
     1861 ccgtttttcc gacaccaggt tcaccgataa ggacaggatt attcttggtt ctacgattga
     1921 gaatctcgat gacacggata atctcatcgt cgcgcccaat aacggggtca atgtctccac
     1981 gacgggcaat ttcagttaca ttaataccaa attcttccag caggcctttt tcttggctag
     2041 gtggcggagt ttgagcagat ccacgatttt gggaaccata accgccgttt ccaccgtaac
     2101 ctccacctga ttgggttggg ggaataggag gagtattgct agaaggtctg aaattgttta
     2161 gatcattgaa gaaatcacca aagggatcga agtcacgatt gttcagatcc gtcatacctt
     2221 tgaagaggct attgttagga tctgttttga taatcttata gcagttttga cagaggtcaa
     2281 tttgtttttg ttttccattg agattggtgt aaagatgaat tgttgagtcg ttaattttac
     2341 agttttgaca aagcatacat ctacctcatt tctttcttta gcctgacttg tttaaaggtc
     2401 aaaaatagtc aaattctata ctctcattat aacaggattg gggtgtaaag caagtaaaaa
     2461 gattgaagct ttgagaagtt gttacaatga aaatttttgt gaatttggac tataaaaaaa
     2521 tcctatttgt gagacaaaaa ggattttact tagacatgca gggacaatcc cagcttgttt
     2581 tagattagta gagaagttcg taaatttcca ttgcgattaa gtcaatgcta tcaaacgtat
     2641 acgtttcgcg atcttctacg tcacgaaggg taaagattgg gccgttttct tcgtcgtggc
     2701 tgtacgcaac ttctgcaaca acaactcctt cgcgttcaaa attacgtttt aaatttccgc
     2761 catcgtttgc cattgcttca aggcggttga tgattctaac caaatgtgat tccattttga
     2821 ttctccttca tttcttatcg ttactatttt accataaagg aggccaactt gactagaaaa
     2881 aactaagatt tctcgctaat tctgctagtt taaatatttt taaataaatt ggataaaaaa
     2941 tttaaatttt aattcaaaac atgttataat catacagtat tctattttag aaagcagtgt
     3001 gactatgaat ttttcttttt tacctaagta tttaccttat tttaactatg gggctgttgt
     3061 gacgattctt atttctatct gtgttatctt tttgggaact attttgggtg ttgtcttggc
     3121 ttttgggcaa cgttcaaagt ttaaaccgct tgtttggttg gccaacttgt acgtttggat
     3181 tttccgtggg acaccgatga tggttcaaat tatgattgcc tttgctctta tgcatatcaa
     3241 tgctccgact attcagattg gaattttagg tgttgatttt tcgcgtctga ttccagggat
     3301 tttgattatc tctatgaata gtggtgctta tgtttcggag actgttcgtg ccggaatcaa
     3361 tgcggttcca aaaggtcagc tagaggcggc ttattcgcta gggattcgtc ctaaaaatgc
     3421 gatgcgttat gtgattttgc cacaagcagt caaaaatatc ttgccagcat tggggaacga
     3481 atttatcacc attatcaagg acagctccct cttatcagct attggggtca tggagttgtg
     3541 gaatggggct acaacagttt ctacaacaac ctatctacct ttaacaccac ttttatttgc
     3601 agcattttac tacttgatta tgacctctat tctgacagta gccttgaaag cttttgaaaa
     3661 acatatggga caaggagata agaaataatg acagaaacct tgataaaaat tgaaaattta
     3721 cataaatctt ttggaaagaa tgaagtattg aagggcatca acctcgagat taaaagagga
     3781 gaagttgtcg ttatcatcgg tccttcaggg agtgggaaat ctaccttgct tcgctctatg
     3841 aatttgttgg aggaagcaac caaggggaag cttatctttg agggagtcga tattacggac
     3901 aagaagaatg acctgtttgc catgcgtgag aagatgggca tggttttcca acaatttaac
     3961 ctctttccta atatgactgt gatggaaaat attaccttgt cacctatcaa gactaaaggt
     4021 gaaagcaggg aagttgctga gaagagagcc caagagcttt tggaaaaagt tggtttgcca
     4081 gataaggcag acgcttatcc acagagtctg tcgggtgggc aacaacaacg gattgccatc
     4141 gcgcgtgggt tggctatgga accagatgtt ttgctctttg acgaaccaac ttcagccttg
     4201 gatcctgaaa tggtaggtga ggtattggct gttatgcaag accttgccaa gtcagggatg
     4261 actatggtta tcgtaacaca tgagatggga tttgcccgtg aggtggcaga tcgtgttatc
     4321 tttatggcag acggtgtggt tgttgaagat gggacacctg agcagatttt tgaacaaacc
     4381 caagaacaac ggactaaaga tttcttgagt aaggttttat aatctatttt aaaatttcaa
     4441 gacaagatta gtgaaaagtt caaccagagc tttttcttat agtttgaaac tataggataa
     4501 cctaggaaag aagtgttaga gggcactagc agtttttggt ataatggaag atatttgaaa
     4561 gaaaagagaa gtgatatgac acagattatt gatgggaaag ctttagcggc caaattgcag
     4621 gggcagttgg ctgaaaagac tgcaaaatta aaggaagaaa caggtctagt gcctggtttg
     4681 gtagtgattt tggttgggga taatccagcc agccaagtct acgttcgcaa caaggagagg
     4741 tcagctcttg cggctggttc ccgtagcgaa gtagtgcgag ttccagagac tattactcaa
     4801 gaggaattgt tagacctgat tgctaaatat aatcaggatc cagcttggca tgggattttg
     4861 gtccagttgc cattaccaaa acatatcgat gaagaggcgg ttttattagc cattgaccca
     4921 gaaaaggatg tggatggttt ccatccccta aacatgggac gtctttggtc tggacatcca
     4981 gtcatgattc cctcgacacc tgcaggaatt atggaaatgt ttcatgaata tgggattgac
     5041 ttggaaggta aaaatgcggt cgtaatcggt cgttcaaata tcgttggaaa acctatggct
     5101 cagcttcttt tggctaaaaa tgcgacagta accttggccc actcacgaac tcataatctt
     5161 gccaaggtgg ctgctaaagc agatattctt gtagtcgcaa tcggccgcgc caagtttgtg
     5221 actgctgact ttgtcaaacc aggtgcggtt gtcattgacg ttgggatgaa tcgagatgaa
     5281 aatgggaagc tctgtggaga tgttgattat gaggcagttg caccacttgc tagccatatc
     5341 acgccagtcc ctggaggtgt cggtcctatg accattacca tgctgatgga gcaaacttat
     5401 caagcagcac ttcggacatt ggatagaaaa taagagaaac attcgattaa tagagtgtat
     5461 tttctatagc tatatctaaa atggcagaaa tgaatattaa attttagata taagattaca
     5521 aaaggaggtc tgcgtctcct ttttattgta taataaaagt gagaggaaaa aaggatgaaa
     5581 gtgattattc aaaccttatt agaaaaagtt attattgaac gttctcgttc aagtcataaa
     5641 ggagattatg gtcgtctgct gctgcttggt ggtacttatc cttatggagg tgccatcatc
     5701 atggctgctt tagcagctgt aaaaagcggt gcaggattgg tgaccgttgg aacggacagg
     5761 gaaaatatcc cggctctgca cagccattta cctgagccta tggccttttc tcttcaagac
     5821 cagcaattgt taaaagagca attggagaag gcagaagttg tcttgctggg gcctggttta
     5881 cgagacgatg cttctggaga aaatctagta aaacaggtct ttgttaattt aagccaaaat
     5941 cagattttga ttgtagacgg aggtgccttg accattcttg ctaggacaag tttgtcattt
     6001 ccctcaagcc agcttatcct aactcctcac caaaaagaat gggaaaaatt atctggaatt
     6061 acgattgaaa agcaaaagga agatgcaacg gctagtgttc tgacttcctt ccctcaagga
     6121 acaattttgg tggagaaagg cccagctact cgtatttggg aagttggcca atctgattat
     6181 taccagttac aggttggtgg tccttatcag gcgactgggg gaatgggaga tactctggct
     6241 gggatgatcg caggatttgt aggccaattc cgacaggcca gtctctacga gcgtgtggca
     6301 gtagcgaccc atcttcattc agccatagcc caagaactat ctcaagaaaa ttatgtggtc
     6361 ttaccgacgg aaatcagcag gtatcttccc aaaataatga aaattatttg tcaacaagag
     6421 agggtaagca aagataaact tgtttaaaat agacagataa atctacttcc ttctcttaat
     6481 ttatgattct tgaaatgcgc ctgctttaag gctaaactag acgtatcaaa gttaagagtt
     6541 tgtttttgta actggaaaga gatgaacaaa tcaaatacat aaaagataaa ctcattttta
     6601 ttcgtttggt aaaaataaaa tgcatatttt taaagaaaga ttgatgaaaa atgtagattt
     6661 gatgttttta cttgaataaa aagcggtttc atagtaaaat aagaactgag aatgatggag
     6721 gtaaatcggt ggaaaatctg aagaaaatgg caggtatcaa ggctgctgag ttcgtgagcg
     6781 atggaatggt cgttggactt ggaacaggct cgactgccta ttattttgtc gaagaaatcg
     6841 gtcgtcgtat caaggaagaa ggattgcaga ttacagctgt aacaacttct agtgtgacca
     6901 ctaaacaggc agaaggcctt aatatcccgc tcaagtctat tgaccaagta gattttgtcg
     6961 atgtgacggt tgatggcgcg gatgaagtgg atagtcagtt taatggaatc aaaggcggtg
     7021 gtggtgccct tctcatggaa aaggtggtcg caacaccatc aaaagaatac atttgggtgg
     7081 tggatgaaag caagctggtc gaaaaactag gtgcttttaa attgccagta gaagtggttc
     7141 agtatggtgc agagcaggtc tttcgtcatt ttgaacgagc tggctacaaa ccaagtttcc
     7201 gtgaaaaaga cggccaacgt tttgtgacgg atatgcagaa ttttatcatt gacctcgcct
     7261 tggatgtcat tgaaaatcca attgcttttg gacaagaatt ggaccatgtc gttggtgttg
     7321 tggagcatgg tttattcaac caaatggtgg ataaggtaat cgttgctgga cgagatggag
     7381 ttcagatttc aacttcaaaa aaaggaaaat agaagggggc ataagatgtc taaatttaat
     7441 cgtattcatt tggtggtact ggattctgta ggaatcggtg cagcaccaga tgctaataac
     7501 tttgtcaatg caggggttcc agatggagct tctgacacac tgggacacat ttcaaaaaca
     7561 gttggtttga atgtcccaaa catggctaaa ataggtcttg gaaatattcc tcgtgaaact
     7621 cctcttaaga ctgtagcagc tgaaatcaat ccaactggat atgcaacaaa attagaggaa
     7681 gtatctcttg gtaaggatac tatgactgga cactgggaaa tcatgggact caacattact
     7741 gagcctttcg atactttctg gaacggattc ccagaagaaa tcctgacaaa aatcgaagaa
     7801 ttctcaggac gcaaggttat tcgtgaagcc aacaaacctt attcaggaac ggctgttatc
     7861 gatgattttg gaccacgtca gatggaaact ggagagttga ttatctatac ttcagctgac
     7921 cctgttttgc agattgctgc ccacgaagac attattcctt tggatgaatt gtaccgtatc
     7981 tgtgaatacg ctcgttcgat tacccttgag cgtcctgccc ttcttggtcg catcattgct
     8041 cgcccttatg taggtgaacc aggtaacttc actcgtacgg caaaccgtcg tgacttggct
     8101 gtatctccat tttccccaac tgttttggat aaattgaatg aggctggtat cgatacttat
     8161 gctgtgggta aaatcaacga tatctttaac ggtgctggta tcaaccatga catgggtcac
     8221 aacaagtcaa atagtcatgg aattgataca ctattgaaga ctatgggact tgctgagttt
     8281 gaaaaaggat tctcattcac aaacctagtt gactttgatg ccctttacgg ccatcgtcgt
     8341 aatgctcacg gttaccgtga ttgcttgcat gagtttgatg aacgcttacc tgaaattatc
     8401 gcagctatga gagagaatga ccttctcttg attactgcgg accatggaaa tgacccaacg
     8461 tatgcaggaa cggatcacac tcgggaatat attccattgt tggcctatag ccctgccttt
     8521 aaaggaaatg gtctcattcc agtaggacat tttgcggata tttcagcgac tgttgccgat
     8581 aactttggtg tggaaactgc tatgattggg gaaagtttct tagataaatt ggtataagat
     8641 gacgcgctat gctttgctgg tgagaggtat caatgttggt ggtaagaata aggtcgtcat
     8701 ggcggagctt cgtcaagaat tgacaaactt gggactggaa aaggttgaga gctacatcaa
     8761 cagtggcaat attttcttta cttcgataga ttccaaagcc caattggttg aaaagctaga
     8821 gactttcttt gcagtccatt atccatttat tcagagcttt tctttactga gtctagagga
     8881 ctttgaggcg gaacttgaaa atctaccagc ttggtggagc agagacttgg cacgaaaaga
     8941 ttttctcttt tacactgagg gtttggatgt ggaccaagtc atcgcgacag ttgaaagttt
     9001 agagctgaaa gatgaagtgc tttattttgg aaaacttggg attttctggg ggaaattttc
     9061 tgaagaatcc tattctaaga ctgcctatca taagtacttg ctgaaggtgc ctttctaccg
     9121 ccacattact attcgtaatg ctaaaacctt tgacaaaatt ggtcaaatgc taaaaaaata
     9181 ataaaggaga cacacaatga catttttaaa caaaatccat gaaactgcta ctttcctgaa
     9241 agaaaaggga attgcagccc ctgagttcgg tctaatcctt gggtcaggac ttggagaatt
     9301 ggcagaagaa atcgaaaatc cagttgtagt agactatgct gagattccaa actggggccg
     9361 ttcaacagta gtcggtcatg ctggtaaatt ggtatatggt gaactggcag gtcgcaaggt
     9421 cttggctctt caaggtcgtt tccatttcta tgaagggaat cctctggaag tggtgacttt
     9481 cccagttcgt gtgatgaaag ttcttggatg tgaaggtgtt attgtaacca atgcagctgg
     9541 cggtatcgga tttggtcctg gtaccttgat ggctatctca gaccatatca acatgacggg
     9601 gcaaaatcca ttgatgggtg aaaacttgga tgactttggc ccacgtttcc cagatatgtc
     9661 tagggcctac acaccagaat accgtgccac tgcccatgaa gtggctaaaa aacttaatat
     9721 caagcttgat gaaggtgtct atatcggagt tactggtccg acttatgaaa caccagcaga
     9781 aattcgttcc tataagacac tgggagcaga tgcagttggt atgtctacgg ttcctgaagt
     9841 tatcgtggca gcccactctg gcttgaaagt tctgggaatt tcatgtatca ctaactttgc
     9901 ggctggtttc caagaagaac tcaatcacga agaagttgta gaagtgactg aacgtgttaa
     9961 aggtgatttc aaaggcttgc ttaaagcgat tcttgctgaa ttgtaagaaa aaagatttaa
    10021 aaggggaagt gcctctgttt tttcaggatt gactgcctat ccggattaaa gaagaaacag
    10081 aggaatacta tgagcttctt cctgctctta taactgaaag aagcggaaga ataggtatgt
    10141 ctgatctgat agccagcatt gtgaaagaca agattctagg atactagcat tagctttcta
    10201 gccaagcaga ctagtatgat aaggagagat gagaatgaat tgactttctg aatttctcag
    10261 tcttatcata tatagcacaa tgagatttcg cttgagtctg cttgtaaata aacgaaaaga
    10321 aagataagaa ataatgaaaa ttggtcaacg aattatgcgc tttggcataa aaaattaagt
    10381 atcggagttg tatctgttgt agtcggcttt gatttctagc tccagctgga atttcagcca
    10441 atgaagtaaa gcaagatgta acatttgaag tggtaatagg tgtgctagat tctaaggagg
    10501 aattgaaaga gtcagaaaat gatgctccaa aactagaaac tcctcttaga gaggagccaa
    10561 gactagctcc tcaaacgctt ccggaagcaa gtgaagttct tgaaaacaaa agggaagagt
    10621 caaaagtaga gataacagag ccagctcaag actcacctat tcttgctcct gtagaagaaa
    10681 ctaaagaaga agctgtgaca gaaaaaccaa caaatactcg ttctctaact gcagaagatt
    10741 tggtgaagat ttccaaaggg gaattgcatt tagaaaatga tttgattgat gaatctttct
    10801 atggtgaaaa agctcttgat ttggaagggg atgatgacca ggatggcatc aaaaacaaag
    10861 atggtaagga ttatctagga tataacagtc atcccttgct agcagacagt gatggggatg
    10921 gtttggcaga tggggaagat gataataaga aagaatggta tgtcacagac cgtgattctc
    10981 ttctctttat ggagttagct tatcgagacg atgattatat tgagaaaatt ttatatcata
    11041 agaatctttt ctctagtctc tatcttgacc gtcaagaaca caaactcatg cacaatgaat
    11101 tggctccttt ctggaagatg aaaaaagcct actatacaga tagtggcttg gatgctttct
    11161 tatttgagac caagagcgac cttccttatc tcaaagatgg aacggtgtac atgttggcta
    11221 ttcgtggaac gcgagttaat gacgccaagg acttgagtgc agatttggtt ttattaggtg
    11281 gaaataaacc agctcaagcg gatgatatcc gcaaggttgt tggggaatta gccaaggata
    11341 taagtattac taagttgtat atgacaggtc attctcttgg aggctaccta gctcagattg
    11401 cagcggttga agattaccaa aaatatcctg atttttataa ccatgtattg aggaaagtga
    11461 caactttcag tgctcctaaa gtcattactt ccagaactgt ttgggatgct aagaatggtt
    11521 tctgagatgt tggtttggaa agtcgtaaat tagctgttag tggaaaaatt aagcattatg
    11581 tggttgataa tgacaatgtt gtgactccct tgattcataa taatcgtgat attgttacat
    11641 ttacaggtaa ttcacgcttt aaacaccgtt ctcgtggcta ttttgaaagt ccaatgaatg
    11701 atattcctaa ctttaatatt ggtaaacaag ctaccttgga taaacatggt tatcgtgatc
    11761 cgaaattgga taaagtgcga ttctttaaga aacaggctct gcctcgatct tttagtcaac
    11821 caagcgctga accaatggaa aatattgcct taggaaaaca ggttactcaa agttcgacag
    11881 ttttcggagg agatgctaga agagctgtgg atggcaaagt cgatggtaac tatggtcaca
    11941 attctgtcac tcatacaaac ttccaatcta agccttggtg gcaagtagat ttggctaaag
    12001 aagaaaccat tcgccaaatc aatatttaca accgaacaga cactgcccag gatagattgg
    12061 caaactttga tgtcattctt ttagacagtt ctggtaaaga aattgagtga aaacgtataa
    12121 catctcctaa agatgtgtca gcacaaatta cgattaacca taaaaaagtg cgctatgttc
    12181 ggattgagct agaaggctat aatgccctca gtcttgcaga agttgaagtt ttccgcttta
    12241 tagctacgaa tgctgaaacg gcgacacaag tttctaagcc agttcaacca atcagtcaga
    12301 ctcctgtgaa agataaaaca ttgacaattc aacacagtgg agcttacatt gtccgctact
    12361 ccataacttg ggaagaagtt ccagtagata aagatggaaa ccaagttgtt cgtagtcatt
    12421 cttgggaagg aaacggttgc aaccagactg caggttttgt cctcaacctc ccaatcaaag
    12481 aaaatatgag aaatctgcga gttaagattg agaaaaagac gggcctacta tggaatagat
    12541 ggcaaacaat ctatgaaaac agaccaattt tagctcaacc ccaccgtaaa attacccatt
    12601 ggggtacgac attgaattcc aaggtgagtg acgatgatgt cttgtaatct gatggtagaa
    12661 tgacagttag tttgtctagt ttataagaaa gtactacctg agcttgaata gaactcaggt
    12721 agctctctat gaaagaacaa aattaatact caatgaaaat caaagagcaa actaggaaac
    12781 tagccgcagg ttgcttaaag cactgctttg aggttgtaga taagactgac gaagtcagtc
    12841 acatatataa tccaaggcga cgttgacgtg gtttgaagag attttcgaag agtataaaca
    12901 gaaaggtaga gcgcgtgttc taatttgaac acgagtagaa aacttttcta aaaacaaaaa
    12961 cgaaaggatg ggtaaactgt attcgctgaa ctgaatacgg gcgactctcc tctaaatcaa
    13021 aattaagaaa gaaaggaatt gaacccaccc taaaagcagt gggaaaaaga tagttggtct
    13081 agcgagcatc gctcactgcg cccaactcct attttccctt cgctttttga tgggtttggt
    13141 atctttctca atataaaata taaaataaag aaaggtagag cgtgtgtttt gatttgaaca
    13201 cgagcggaaa actcggaaaa tagataatct gactgaaaaa tcaggatttc tcgtcaggtt
    13261 cctaattttc agtcgttttc ttctcgctct ttgtatcata aattatgtct atccatattg
    13321 ctgctcagca gggtgaaatt gctgataaaa ttcttcttcc tggggatcct cttcgtgcta
    13381 agtttattgc ggagaatttc cttgatgatg ctgtttgttt taacgaagtg cgtaacatgt
    13441 ttggttacac tggtacttac aagggtcact gtgtatctgt catgggaact gggatgggaa
    13501 tgccatctat ttcgatttat gcgcgtgagt taatcgtaga ctacggtgtg aagaaattga
    13561 ttcgtgtggg aactgcaggt tctttgaatg aagaggttca tgttcgtgaa ttagttttgg
    13621 cgcaggcggc tgcaaccaac tcaaacatcg ttcgtaatga ctggccacag tacgattttc
    13681 cacaaattgc tagctttgat ttgcttgata aagcctacca tatcgccaaa aaacttggta
    13741 tgactactca cgttgggaac gttttgtcat ctgatgtctt ttactcaaat tactttgaaa
    13801 agaatatcga gcttggtaaa tggggagtca aggctgtgga aatggaagca gcagctcttt
    13861 actatcttgc tgcccaatac catgttgatg cgctagctat catgaccatc tctgatagct
    13921 tggtcaatcc agacgaagac acaactgcag aagaacgtca aaataccttc actgatatga
    13981 tgaaggttgg tttggaaacc ttgattgcag aataattata gccaaaaagg aagcccttta
    14041 tgaattgtag gacttccttt tcttatccag aaattgatct agctctctct gatttcgaag
    14101 gatagtgact ttatgtgaat attcttggca aagtttttgg taattttctt tttgagtttt
    14161 gctacgccca tcccaaagaa tccatctgat aaactcccac tcaaagcgtt cagggcaatc
    14221 tgccgccata ctttctctga cttttccacg gtatttaaga taacgcttaa aggctctaaa
    14281 gagacaggtc aatggcaaaa aattgagaaa gatgatttgg tcagcttctt gcattcgttc
    14341 ttggtagtag caccaagaat aattaccatc gatgacccaa gctttatgct tggtgagaaa
    14401 gttttttatc tcggttaaca tccattcgca gtcactgtct tgccaaccag gttgaaattg
    14461 gagtgtgtcc atgtgcagtt ttggaatgga gtagtagtta gataactttt ctgctagagt
    14521 tgacttacca gaaccagaat atccgataat tgcgattttc attttctacc ttttcctatt
    14581 tggagacaaa aaaacagcct ctatggactg tttcttattt aacaagttta gctgaaagac
    14641 gagctttatc gcggcttgct ttgtttttgt gaatcaaacc tttagtttct gctttatcga
    14701 tagctgagct agcagcacgg aaaagttctt cagatgggtt tgcttcgaaa gcttttatag
    14761 cagtacgcat agctgatttt tgagctgagt tcttttcgtt ttgtttaacg ttcaattcag
    14821 cgcgtttgat agctgattta atgtttgcca atgttcttac ctccatattt actaactata
    14881 ccattatata tgaaaactta cgttttgaca agggaaaatt acttttttaa gtaaatttca
    14941 tcgatttcat ggttcttgct tttatgaaga atcacttctg cacgatttct ggttggttca
    15001 atataatttt gcagatttgt gagattgata ctgatccaga cctgatgggc aaaggactcc
    15061 acttccccaa tcggcatctg agtaaaacga taatagtagc tatcagggtc gttttgggct
    15121 aggctcagca ttttcaagaa acggtccaga taccaacttt caatatcatc cactccagca
    15181 tctacataga tggaaaagtc aaagaagtca gtgatataga gacgatcgtt ttgtggattt
    15241 tgaaagacat taattccctc aacaattaca aaatcagcag ctttgacact ttgtttcttt
    15301 tcgggtacga tgtcgtaaac ttcatgagaa tagacaggaa tatctacatc ttgtccattt
    15361 ttgatgcggt ccaagaagtt gagaagagct tccatatcat agctttcagg aaatccttta
    15421 cgatttaaaa tcccctgctc aatcaaggtt tgattgggat agagaaaacc atcagttgta
    15481 accaactcaa ccgtagcatc tgtaaacgta cgggacagta ggatttgaag taggcgactg
    15541 gttgtggatt ttccaacggc aacactccca gaaaccccaa taataaaagg ttgagattta
    15601 ctttcacgtt ggaggaaaat tccttttgaa aaggctaaat cttccttagt tcgcttgtaa
    15661 atctgaatca gatgagccaa ggggagatag atatctgtaa cgtcttggag actgatttgg
    15721 tcattaaaac tcttgataga ttccaattct tcttctgtca aaggaggtgt tgtctttcga
    15781 tgtaaagatt gccaagtctg gcggctgatt ttttcaaaat gtaaaaattc gttggtcatt
    15841 tgttttcccc tagatatttt gtctatcata ccataaaaag ctaaaaaaat aaagcggttt
    15901 cttgaatgta gtaagtcaat ggtatatagt ataagtgatc aatttattga tgaaatagga
    15961 gatagattct actgatttat aagcttataa agggggcttt ttagttcaca acttatagga
    16021 ggtgtactat gaaaatctta aaacgttaca tattggaact ctgttttatt ttaagttttg
    16081 ctttaccttt tataaaagga accaatgcag ataatggtag atgctttgtg gaaacctatt
    16141 acggttttac ttttttgatg gaacatgcta ttgtaacagc tgtctttatc tgttcgttct
    16201 taattgcttt cttactaaaa aacgatggac gaaatggatt gctgcgggta gttattgctt
    16261 tttagtccta cggattgcga cagagggtta tttcttccac atgtcactag aagatttgat
    16321 acgactttgg acaagtttgg aaatcctgac acaaacgtat cagttgggct tttatctaaa
    16381 cattttgtta ggaattttgt tgataataaa gtattttaag gttaagcaat agaaataata
    16441 atctgcctga ttatagatga taaacttttt gtttttatct ggactgtgat atttatagtt
    16501 ctaaatggtt tattatttat aagtgttgaa agagattgaa gaaaatggct tcgtgacttg
    16561 ctttcttcgg tctttttcgt tttattatca atttgtaaac gatttacaat ttgagtcaaa
    16621 ttatggtaaa atagtgtcat gagtaaaatg tactatgcag aaaatcctga cgctgctcac
    16681 gacattcatg agttgagagt ggacttgttg ggagaaaaaa tgactttttt gacagatgcg
    16741 ggtgttttta gcaagaaaat ggttgacttt ggaagtcaac tcttgctcaa gtgtctggat
    16801 gtcaaccaag gagagacagt cctagatgta ggctgtggct atggtccatt aggtttgtcc
    16861 ttggtcaagg tttacggagt tcaggcgacc atggttgata ttaacactcg tgccctggat
    16921 ttagctcaga gaaatgctga aaaaaataat gcaaaagcga cgatatttca atctaatatt
    16981 tatgcaagag ttcaagggcg atttgaccat gtcatttcca atcctcctat ccgtgcgggc
    17041 aagcaagtcg ttcatgaaat catcgagaaa agtaaagatt tcttggaaat tggtggagat
    17101 ttgaccatcg tcatccagaa aaaacaagga gctccaagtg ccaagtctaa gatggaagat
    17161 gtgtttggca attgtgaaat ccttaaaaaa gacaagggat actatatcct tagaagtgtg
    17221 aaagaatgag agcagttgat ttaatccaaa aaaaacgaga cggtcaagaa ctgacttcaa
    17281 gtgaaattga atggctgata gaaggctatg tatcaggaac tgttcctgat tatcagatgt
    17341 ctgcctttgc tatggctgtt tattttaaag gaatgacgac tcgagaaatc tctgatttaa
    17401 cgatgaatat ggttaagact gggcaagagt ttgacctgtc agccattgat ggtgtgaaag
    17461 ttgacaagca ttctactggt ggtgtcggcg ataaggtaac cttgatttta gctcctcttg
    17521 ttgctagctt cggtgtgcct gtagcaaaaa tgagtggtcg tggtcttggc catacaggcg
    17581 gaacaattga taaattggag tccattaagg gctatcaagt agaacgtagt caagaggatt
    17641 ttattcgtca ggttcaggat attggagtat ctgtcattgg gcaatcagac cagctggtca
    17701 aagcagataa gcttctctat gcccttcgtg atgtgaccgc aactgtcgac acgattcctt
    17761 tgattgcgag ttcggtgatg agcaagaaaa ttgcggcagg ggcggatgct attttgctag
    17821 acgtgactgt cggtgagggt gccttcatga agacggttga tgaggcgcgt gagttggctc
    17881 aaactatggt agatcttggt aaggttgttg gacggaagac agtagcagtg attacggata
    17941 tgagccagcc tttgggacga gcgattggaa atcgtctgga aatccttgaa gcattggaga
    18001 ttttgcaagg tcaaggacgt caggatatta cccactttat ttgtgaattg gctcaaatta
    18061 tgcttggttt ggcaaatgtt aacaaaactg ttgaagaagt tcgccaacat cttgaaaatg
    18121 gtcaagcact ggctaagttt gaggaaatgg tgcaagccca aggtggagac ttggaagacc
    18181 tctatcgtcc tgtacatgta gctcatgtgg tggaaatccc tgctcaggaa acgggtgtca
    18241 tttcagctct tccagctatg gactttggtc tttatgccat gaggctgggt gctggtcgtg
    18301 cagttaagtc tgatgtcttg gactatgaaa caggaattgt ttttgaaaag aagattggag
    18361 actccgttca aaagggtgaa attgttgcaa aagtatacac aaatgaaaaa attccacctc
    18421 aactagttac agatttccaa aaatgtgtta aaataagtga tgaagtgaaa aaaatccgtg
    18481 aaattgtaga aatcatctcc taaatggagg gaaaaccgaa atgaaattaa ataaatatat
    18541 agatcatacg cttttaaaac aagatgcaaa gaaaaaacaa attgatagtt tgttgtctga
    18601 ggctagagag tatgactttg ccagtgtttg cgttaatccg acctgggttg aacatgctaa
    18661 aaaaggactt gaaggcacag atgttaaggt ttgcacagta gtaggtttcc ctttgggagc
    18721 aacaacttca gccgtgaaag catttgagac aaaagaagct atccaaaatg gtgcagatga
    18781 gattgatatg gtgatcaatg ttggagctct caaatcaggt aatttagcct tggttgagtc
    18841 agatattcgc gcagtagtgg aagcaagtgg tgataagtta gtgaaagtca ttattgaagc
    18901 ttgccttctg acagaccaag aaaaaattgt tgtttgccaa ttggcccaaa aagctggggc
    18961 tgactttgtt aaaacatcta ctggcttttc aactggtggt gctacgatag cagatgttag
    19021 attaatgcgt gaaacagttg gatctgatat gggagtcaag gctgctggtg gagctcgttc
    19081 ttatgcagat gctcttgcct ttgtcgaagc aggtgcgacc cgtatcggaa cgtcagctgg
    19141 ggtagctatt ttaaaaggag aattggcaga tggcgactac tgagttgatt gaactggcaa
    19201 ttgaaaccag caaacatgcc tatgtcccct attctcactt tcctatcgga gcagttttag
    19261 tagccaaaga cggaagtgtt tacacgggag tgaatatcga aaatgctagc tatcctttga
    19321 ccaattgcgg tgagagaaca gctgttttta aggctatatc tgaaggccaa agagaatttt
    19381 cagaattgat tgtctatggt caaactgaaa aaccgatctc gccatgtggt gcttgtcgcc
    19441 aagtaatggt cgaatttttt gaacaagatt taaaagtgac cttagtcgca aaagataaat
    19501 cgacggtcga gatgacggtc ggggaattac ttccatactc ttttacagac ttaaactagt
    19561 ctgagtcgct ctctgagtgg cacggtcctt gtggccaacc aatccatact tgcaacatcg
    19621 ttgcacatct tatttaggag gttcagtaat gaacaagaaa caatggctag gccttggtct
    19681 agttgcagtg gcagcagttg gacttgctgc atgtggtaac cgctcttctc gtaacgcagc
    19741 ttcatcttct gatgtgaaga caaaagcagc aatcgtcact gatactggtg gtgttgatga
    19801 caaatcattc aaccaatcag cttgggaagg tttgcaggct tggggtaaag aacacaatct
    19861 ttcaaaagat aacggtttca cttacttcca atcaacaagt gaagctgact acgctaacaa
    19921 cttgcaacaa gcggctggaa gttacaacct aatctttggt gttggttttg cccttcataa
    19981 tgcagttgaa gaagcagcta aagaacacac tgacttgaac tatgtcttga ttgatgatgt
    20041 gattaaagac caaaagaatg ttgcgagcgt aactttcgct gataatgagt caggttacct
    20101 tgcaggtgtg gctgcagcaa aaacaactaa gacaaaacaa gttggttttg taggtggtat
    20161 cgaatctgaa gttatctctc gttttgaagc aggattcaag gctggtgttg cgtcagtaga
    20221 cccatctatc aaagtccaag ttgactacgc tggttcattt ggtgatgcag ctaaaggtaa
    20281 aacaattgca gccgcacaat acgcagccgg tgcagatatt gtttaccaag tagctggtgg
    20341 tacaggtgca ggtgtctttg cagaggcaaa atctctcaac gaaagccgtc ctgaaaatga
    20401 aaaagtttgg gttatcggtg ttgatcgtga ccaagaagca gaaggtaaat acacttctaa
    20461 agatggcaaa gaatcaaact ttgttcttgt atctactttg aaacaagttg gtacaactgt
    20521 aaaagatatt tctaacaagg cagaaaaagg agaattccct ggcggtcaag tgatcgttta
    20581 ctcattgaag gataaagggg ttgacttggc agtaacaaac ctttcagaag aaggtaaaaa
    20641 agctgtcgaa gatgcaaaag ctaaaatcct tgatggaagc gtaaaagttc ctgaaaaata
    20701 atggatggaa gtcatttctt aggaagcggc caagggccgc ttttttaaga gttgagacaa
    20761 agtcattttg tctcagtaaa gcttgctact agacaaacta gcaagtttta ttgaaataaa
    20821 ataagactct gaaaggaaga gcacatggca cacgaaaatg tcattgagat gcgtgatatt
    20881 accaaggtgt ttggtggatt tgttgctaac gacaaaatca acttgcacct acgaaaaggt
    20941 gaaattcatg cacttttagg agaaaatggg gctggtaagt ccacgctaat gaacatgtta
    21001 gcaggccttc ttgaaccaac tagtggtgaa atcgcggtca acggtcaagt tgtcaatctc
    21061 gactccccat ctaaagcagc tagcttggga atcgggatgg ttcaccagca ctttatgttg
    21121 gttgaagcct tcacagtggc tgaaaacatc attttaggta gtgaattgac taaaaatggt
    21181 gtgctagata tcgctggagc tagcaaagaa atcaaggctc tttctgaacg ttatggctta
    21241 gctgttgacc cttctgccaa ggtagcagat atctcagttg gagcccaaca acgtgtagaa
    21301 attttaaaaa cactttatag gggggctgat atccttatct ttgacgaacc aacggctgtt
    21361 ttgactccat cagaaattga tgagttgatg gctattatga aaaatcttgt caaagaagga
    21421 aaatcaatta tcttgattac ccacaaattg gatgaaatta gcgcagtttc tgaccgtgtt
    21481 acagttatcc gtcgtgggaa atcaattgaa accgttgaaa ttgcaggggc taccaatgct
    21541 gatttggcgg aaatgatggt aggacgttct gtttccttta aaacagagaa gcaagcctct
    21601 aaaccaaaag aagtggtttt gtctatcaaa gatttggtgg tcaatgaaaa ccgtggtgtt
    21661 ccagctgtta aaaatctgtc cttggatgtt cgtgctggag agattgttgg tattgcgggg
    21721 attgatggaa atggtcagtc tgaactgatt caagccatta caggtcttcg taaggttgaa
    21781 tctggtagca ttgagctaaa aggagattca attgtaggct tgcacccacg tcagattaca
    21841 gaactaagtg ttgggcacgt tccagaagac cgtcaccgtg atggcttgat tttggaaatg
    21901 atgatatctg aaaatattgc ccttcaaacc tactataaag aaccacatag taaaaatgga
    21961 attttgaatt attcaaatat tacttcttat gctaaaaagc tgatggaaga gtttgatgtt
    22021 cgcgctgcca gtgaattagt tcctgcagct gcactctcag gaggaaatca acaaaaagca
    22081 attattgctc gtgaaattga tcgagatcct gatctcctta tcgttagcca gccaactcgt
    22141 gggttggatg tcggtgccat tgagtatatc cacaaacgct tgattgaaga gcgtgataat
    22201 ggcaaggctg tccttgttgt cagctttgaa ttggatgaga ttttaaacgt ctcagaccgt
    22261 attgccgtta tccacgatgg taagattcaa ggtattgtat caccagaaac aaccaataaa
    22321 caaggacttg gtgtcttgat ggctggtgga aacttgggaa aggagaagag tgatgtctaa
    22381 aaaattacaa caaatttcgg ttcccttgat ttctgtattc ctaggaattt tactcggagc
    22441 cattgtcatg tggatcttcg gttatgatgc tatttggggc tacgaagaat tgttctatac
    22501 agcctttggc agtctgcgtg ggattggaga aatcttccgt gctatgggtc ctctggtctt
    22561 gattggtctt ggttttgcgg ttgccagtcg agctggtttc tttaacgtcg gacttcctgg
    22621 tcaggctttg gcaggttgga ttctcagtgg ttggtttgcc ctgtcgcatc cagatatgcc
    22681 ccgtcccttg atgattctag caaccatcgt gattgccttg attgctggtg ggattgtcgg
    22741 agcgattcca ggtattctta gggcctatct agggacgtca gaggttattg taaccatcat
    22801 gatgaactac attatcttgt atgtagggaa tgcctttatc catgctttcc ctaaagactt
    22861 catgcaaagt acagattcga ccattcgtgt tggggctaat gcaacctatc agacaccttg
    22921 gttgactgag ttgactggta actcacggat gaatattggt attttctttg ccatcattgc
    22981 cgttgcagtt atttggttca tgctcaagaa aacaactctt ggttttgaaa tccgtgcagt
    23041 tggtcttaat ccacatgctt cagaatatgc tggtatttct gccaagcgga ctattatcct
    23101 atctatgatt atttcaggtg ccttggcagg tcttggtgga tctgttgaag gtttgggaac
    23161 cttccagaac gtctatgttc aaggttcgtc attagctatc ggatttaacg gaatggcggt
    23221 tagtttgctt gcggccaact caccaattgg tatactcttt gcagccttcc tatttggcgt
    23281 tctccaagtt ggggctcctg gtatgaatgc ggcgcaggta ccatctgagc ttgtcagcat
    23341 tgtaacagcg tctattatct tctttgtcag tgttcattac cttatcgaac gctttgtcaa
    23401 accgaaaaaa caagttaaag gaggtaagta aagatgtcta ttataacctt gctccccctc
    23461 ttggtgtctt ctatgctgat ttactcagca cctctcatct ttacaagtat cggtggtgtt
    23521 ttctctgaac gtggtggtgt ggtaaacgtt ggccttgaag gaattatggt tatgggtgcc
    23581 ttttctggag ttgtctttaa ccttgaattt gcagaacaat ttggagcagc aactccatgg
    23641 ctatccttgc ttgtagcagg attggttggt agtgttttct ctatcatcca cgcagcagcg
    23701 acggttcatt tccgtgcaga ccatgttgtc agcggtacgg tattgaactt gatggcgcct
    23761 gccttggctg ttttcttagt taaagttctt tataacaaag gacaaaccga caacctaagt
    23821 caaacttttg gacgctttga tttcccagtc ttggcaaata tcccagtgat tggtgatatc
    23881 ttcttcaagt caactagtct acttggttat ctggcgattg ccttctcatt ccttgcttgg
    23941 tttattctct ttaagactca atttggtctt cgtctccgct ctgtcggtga acatcctcaa
    24001 gcagcggata ccttgggaat caacgtctac aagatgagat atttagggat tattatttca
    24061 ggttttctag gtggaattgg cggagcgatt tatgcgcaat caatctcagt taacttctca
    24121 gtgacaacta ttgttggacc tggatttatc gcccttgctg cgatgatctt tgggaaatgg
    24181 aatccaatcg gtgctatgct ttctagtctc ttctttggac tttcacaaag tttggctgtt
    24241 atcggttctc aattgccgtt cctacaagga gtgccagcgg tttatcttca aattgcacct
    24301 tatgttttga caattcttgt cttagcagcc ttctttggaa aagcagtcgc accaaaagca
    24361 gatggtatca actacatcaa atcaaaataa gcatacaaaa aaacgtcagt ccaatgactg
    24421 aactgccccc caaaagttag acagaaaaaa tctaactttt ggggtgtttt tattatgaaa
    24481 ctaactt
//
LOCUS       DAVKJD010000020        24118 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKJD010000020 DAVKJD010000000
VERSION     DAVKJD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24118
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.020"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            17..673
                     /locus_tag="VW157_000899"
     CDS             17..673
                     /locus_tag="VW157_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9552224.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            781..1876
                     /gene="prfB"
                     /locus_tag="VW157_000900"
     CDS             join(781..852,854..1876)
                     /gene="prfB"
                     /locus_tag="VW157_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9552225.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            1894..2586
                     /gene="ftsE"
                     /locus_tag="VW157_000901"
     CDS             1894..2586
                     /gene="ftsE"
                     /locus_tag="VW157_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9552226.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDIPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            2579..3505
                     /locus_tag="VW157_000902"
     CDS             2579..3505
                     /locus_tag="VW157_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552227.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKV"
     gene            3791..5971
                     /locus_tag="VW157_000903"
     CDS             3791..5971
                     /locus_tag="VW157_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9552228.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAADSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            6023..6838
                     /locus_tag="VW157_000904"
     CDS             6023..6838
                     /locus_tag="VW157_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9552229.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYNLICFQEINQEM
                     TSSEVEVNDLYQALPAAESIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            6978..8321
                     /locus_tag="VW157_000905"
     CDS             6978..8321
                     /locus_tag="VW157_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9552230.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVSRQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGCLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVKNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            8698..9888
                     /locus_tag="VW157_000906"
     CDS             8698..9888
                     /locus_tag="VW157_000906"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9552231.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVINK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            10441..11376
                     /locus_tag="VW157_000907"
     CDS             10441..11376
                     /locus_tag="VW157_000907"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9552232.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            11410..12447
                     /locus_tag="VW157_000908"
     CDS             11410..12447
                     /locus_tag="VW157_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9552233.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            12620..13225
                     /locus_tag="VW157_000909"
     CDS             12620..13225
                     /locus_tag="VW157_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9552234.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADIESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            13382..13912
                     /locus_tag="VW157_000910"
     CDS             13382..13912
                     /locus_tag="VW157_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9552235.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISCLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            13933..15018
                     /gene="rlmN"
                     /locus_tag="VW157_000911"
     CDS             13933..15018
                     /gene="rlmN"
                     /locus_tag="VW157_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU9552236.1"
                     /translation="MKPSIHSLTRQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVDCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFVCTINDDKGMAIGARHITVSTSVLAHKIRNFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     FAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEYGT
                     DIDAACGQLRSNTMKRDRQKAVAEVNP"
     gene            15020..15493
                     /locus_tag="VW157_000912"
     CDS             15020..15493
                     /locus_tag="VW157_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9552237.1"
                     /translation="MTKKRELILRLGVAVYSLCIVYFCFTPQATVEVGTPGIQHLGRL
                     VFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKKVLL
                     FSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN"
     gene            15495..17036
                     /locus_tag="VW157_000913"
     CDS             15495..17036
                     /locus_tag="VW157_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9552238.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNFTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(17092..17895)
                     /locus_tag="VW157_000914"
     CDS             complement(17092..17895)
                     /locus_tag="VW157_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9552239.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(17978..18190)
                     /locus_tag="VW157_000915"
     CDS             complement(17978..18190)
                     /locus_tag="VW157_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552240.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            complement(18669..18878)
                     /locus_tag="VW157_000916"
     CDS             complement(18669..18878)
                     /locus_tag="VW157_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552241.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMDL
                     LADDSLAGDIILLWRIQFGTFTTET"
     gene            19048..19320
                     /gene="rpsP"
                     /locus_tag="VW157_000917"
     CDS             19048..19320
                     /gene="rpsP"
                     /locus_tag="VW157_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9552242.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            19340..19579
                     /locus_tag="VW157_000918"
     CDS             19340..19579
                     /locus_tag="VW157_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9552243.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            19809..20052
                     /locus_tag="VW157_000919"
                     /pseudo
     CDS             19809..20052
                     /locus_tag="VW157_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            20156..20944
                     /locus_tag="VW157_000920"
     CDS             20156..20944
                     /locus_tag="VW157_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9552244.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKCQALVAIEPTTKKVMFADFSENPYPEMEEQMRLIDEYGPELYFKNLT
                     QATFSPETNKEIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            20975..21493
                     /gene="rimM"
                     /locus_tag="VW157_000921"
     CDS             20975..21493
                     /gene="rimM"
                     /locus_tag="VW157_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9552245.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPSKRV
                     DVEILEGLDDED"
     gene            21483..22202
                     /gene="trmD"
                     /locus_tag="VW157_000922"
     CDS             21483..22202
                     /gene="trmD"
                     /locus_tag="VW157_000922"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9552246.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            22214..22552
                     /locus_tag="VW157_000923"
     CDS             22214..22552
                     /locus_tag="VW157_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552247.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(22583..22903)
                     /locus_tag="VW157_000924"
     CDS             complement(22583..22903)
                     /locus_tag="VW157_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552248.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            23001..23216
                     /locus_tag="VW157_000925"
     CDS             23001..23216
                     /locus_tag="VW157_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9552249.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(23285..23365)
                     /locus_tag="VW157_000926"
                     /pseudo
     CDS             complement(23285..23365)
                     /locus_tag="VW157_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(23421..23957)
                     /locus_tag="VW157_000927"
     CDS             complement(23421..23957)
                     /locus_tag="VW157_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9552250.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
BASE COUNT         7445 a         4309 c         5261 g         7103 t
ORIGIN      
        1 gaaaaggaga agtctcatgg cagttaaaga ttttatgacc cgcaaggtag tttatattag
       61 tccagataca acagtatctc atgcagcaga tttgatgaga gagcaaggtt tgcaccgtct
      121 gcctgttatc gaaaatgatc aattagttgg tttggtgact gagggaacca ttgcacaagc
      181 aagtccatct aaagcaacaa gtctttctat ctatgagatg aattatcttc tgaataagac
      241 aaaagtaaaa gatgtcatga ttcgcgatgt tgtcactgtc tcaggctatg ctagtctaga
      301 agatgcaact tatctgatgt tgaaaaataa gattagtatt ctccctgtcg tagataacca
      361 tcaagtatac ggagttatta ctgaccgtga cgttttccaa gcctttcttg aaattgcagg
      421 ttatggcgaa gaagggattc gtgtacgctt tgttacagaa gatgaagttg gtgttcttgg
      481 aaaaattgtt tctttgattg tagaagaaaa tttgaatatc tcccatacag tcaatattcc
      541 gcgtaaggat ggtaaggtga ttatcgaagt gcaaatcgat ggatcaattg atttaccagc
      601 cttgaaagaa aaatttgaag caaatggtat tcaagtggaa gaaatcgctc gcacttcagc
      661 aaaagtcttg taagaaggga agcccaaagg cttctttttt catgaaaagg ggattagaga
      721 aaaagatgga aagaaatgat aaaatatgct ataatgaaat aatgtaaaaa aggagtattt
      781 atggacattt cagtaattcg tcagaaaatt gacgcaaatc gtgaaaaatt agcttctttc
      841 agggggtctc tttgacctcg aagggctaga ggaagagatt gccatcttgg aaaacaagat
      901 gacagaacct gatttttgga acgataatat tgcagcccaa aaaacgtcgc aagaattaaa
      961 tgaattaaaa aacacttaca ataccttcca taagatggaa gagttgcagg atgaagtcga
     1021 aattttattg gattttttgg ctgaagacga gtcagtgcat gatgaactgg tagcgcagtt
     1081 agccgaactt gataagataa tgaccagcta cgagatgact ctactcttgt cagaacctta
     1141 tgaccacaac aatgccatct tggaaatcca tccaggttct ggtggtactg aggcgcagga
     1201 ctggggtgat atgttgcttc gtatgtatac tcgttatggt aatgctaaag gctttaaagt
     1261 ggaagtgttg gattaccaag caggtgatga ggctggtatt aagtcggtaa ctttatcatt
     1321 tgaagggcct aatgcctatg gtctcctcaa gtcagaaatg ggtgttcacc gcttagtgcg
     1381 aatctcacca tttgactctg ccaaacgtcg ccatacctct ttcacatctg tagaagtgat
     1441 gccagaattg gatgatacta ttgaagtgga aatccgtgaa gatgatatca agatggatac
     1501 cttccgttca ggtggtgccg gtggacaaaa cgtcaataag gtttcaacag gtgtacgttt
     1561 aacccacatt ccaactggaa ttgttgtcca atcaacagtg gatcgtaccc agtatggaaa
     1621 tagagatcgt gccatgaaga tgttgcaggc taagctctat caaatggagc aagagaagaa
     1681 ggctgcagag gtagattctc tcaaaggtga gaaaaaggag atcacttggg gaagccaaat
     1741 ccgttcttat gtcttcacgc cttatactat ggtaaaagat caccgaacta gctttgaggt
     1801 tgctcaggta gataaggtta tggatgggga cttagatggt tttatcgatg cttatctcaa
     1861 gtggcgaatt agctaagata gaaaggaact cacatgtcaa ttattgaaat gagagatgtc
     1921 gttaaaaaat acgacaacgg aacgactgct ctacgcggtg tttcggttag cgttcaaccg
     1981 ggggaatttg cttacatcgt aggaccttca ggagcaggga agtcaacttt tattcgttct
     2041 ctgtatcgtg aagtaaaaat cgataaagga agcctatcag ttgctggttt taatctggtt
     2101 aagatcaaaa agaaagatat cccgcttcta cgtcgtagtg ttggggttgt cttccaggat
     2161 tataaattgt taccaaagaa aactgtctat gaaaatattg cttacgctat ggaagtaatc
     2221 ggggaaaatc gccgtaatat caaaagacga gtgatggaag ttttggactt ggttggattg
     2281 aagcataagg ttcgttcttt cccaaatgaa ctctcaggtg gggagcaaca gcggattgcg
     2341 attgcgcgtg caattgtaaa taatcccaaa gtattgatag ctgatgagcc aacaggaaat
     2401 ctggatccgg ataattcatg ggaaattatg aatcttttgg aacggattaa cctacaagga
     2461 acaactattt tgatggcgac tcataatagc cagattgtaa ataccttgcg ccaccgtgtc
     2521 attgccattg aaaatggccg tgtcgttcgt gacgaatcaa aaggagagta tggatacgat
     2581 gattagtaga ttttttcgcc atttatttga agccttaaaa agtttgaaac gaaatggttg
     2641 gatgacagta gctgctgtca gttcagtcat gattactttg accttggtgg caatatttgc
     2701 atctgttatt ttcaatacag cgaaactagc tacagatatt gaaaataatg tccgtgtagt
     2761 agtttatatc cgaaaggatg tggaagataa tagtcagaca attgaaaaag aaggtcaaac
     2821 tgttacaaat aatgactacc acaaggtata tgattctttg aagaacatgt ctacggttaa
     2881 aagtgttacc ttttcaagta aagaagaaca atatgaaaaa ttaaccgaga taatgggaga
     2941 taactggaaa atctttgaag gagatgccaa tcctctctat gatgcctata ttgtagaggc
     3001 aaacactcca aatgatgtaa aaactatagc cgaagatgct aaaaaaattg aaggtgtctc
     3061 tgaggttcaa gatggcggtg ccaatacaga aagactcttc aagttagctt catttatccg
     3121 tgtttgggga ctagggattg ctgctttgtt aatttttatc gcagttttct tgatttcaaa
     3181 taccattcgt attaccatta tttcccgcag tcgcgaaatt caaatcatgc gcttggtcgg
     3241 agctaaaaac agttatatcc gtggaccgtt cttgttagaa ggagccttta tcggtttatt
     3301 gggagctatc gcaccatctg ttttggtctt tattgtttat caaattgttt accaatctgt
     3361 caacaaatcg ttggtagggc aaaatctatc catgattagt ccagatttat ttagtccgtt
     3421 gatgattgcc ctactatttg tgattggggt tttcattggt tcattgggat caggaatatc
     3481 catgcgccga ttcttgaagg tttaggtaaa atagctgctt ttatgaggag attgtaaaat
     3541 ctcctttttt gctacaagag tttttgaaaa gagatgcgca gaagaaaaga gcttccaaag
     3601 aagtccccca gagaagactt cttgatggct aatttgaact tgaaatttgg cgcactagaa
     3661 attttttaga aaaaagtgta atttttttat aaaagccttt acaaaaaaaa ttaaagtggt
     3721 ataattaaat catagaaagt gcaaacgttt tcgtaaaacg tttgcctaaa taaaaataaa
     3781 ggagatttga atgatgaaag atacattcaa aaatgtcttg tctttcgaat tttggcaaaa
     3841 attcggtaag gctttgatgg tagttatcgc ggttatgccg gctgctggtt tgatgatttc
     3901 aatcggtaag tctatcgtga tgattaaccc aacctttgca ccacttgtca tcacaggtgg
     3961 aattcttgag caaatcggtt ggggggttat cggtaacctt cacattttgt ttgccctagc
     4021 cattggagga agctgggcta aagaacgtgc tggtggtgct ttcgccgctg gtcttgcctt
     4081 catcttgatt aaccgtatca ctggtacaat ctttggtgta tcaggcgata tgttgaaaaa
     4141 tccagatgct atggtaacta ctttctttgg tggttcaatc aaagttgctg attactttat
     4201 cagtgttctt gaagctccag ccttgaacat gggggtattc gtagggatta tctcaggttt
     4261 tgtaggggca actgcttaca acaaatacta caacttccgt aaacttcctg atgcactttc
     4321 attcttcaat gggaaacgtt tcgtaccatt tgtagttatt cttcgttcag caatcgctgc
     4381 aattctactt gctgctttct ggccagtagt tcaaacaggt atcaataact tcggtatctg
     4441 gattgccaac tcacaagaaa ctgctccaat tcttgcacca ttcttgtatg gtactttgga
     4501 acgtttgctc ttgccatttg gtcttcacca catgttgact atcccaatga actacacagc
     4561 tcttggtggt acttatgaca ttttaactgg tgcagctaaa ggtactcaag tattcggtca
     4621 agacccacta tggcttgcat gggtaacaga ccttgtaaac cttaaaggta ctgatgctag
     4681 tcaatatcaa cacttgttag atacagtaca tccagctcgt ttcaaagttg gacaaatgat
     4741 cggttcattc ggtatcttga tgggtgtgat tgttgctatc taccgtaatg ttgatgctga
     4801 caagaaacat aaatacaaag gtatgatgat tgcaacagct cttgcaacat tcttgacagg
     4861 ggttactgaa ccaatcgaat acatgttcat gttcatcgca acacctatgt atcttgttta
     4921 ctcacttgtt caaggtgctg ccttcgctat ggctgacgtc gtaaacctac gtatgcactc
     4981 attcggttca atcgagttct tgactcgtac acctattgca atcagtgctg gtattggtat
     5041 ggatatcgtt aacttcgttt gggtaactgt tctctttgct gtaatcatgt actttatcgc
     5101 aaacttcatg attcaaaaat tcaactacgc aactccaggg cgtaacggaa actacgaaac
     5161 tgctgaaggt tcagaagaaa ccagcagcga agtgaaagtt gcagcagact ctcaagctgt
     5221 aaacattatc aaccttcttg gtggacgtgt aaacatcgtt gatgttgatg catgtatgac
     5281 tcgtcttcgt gtaactgtta aagatgcaga taaagtagga aatgcagagc aatggaaagc
     5341 agaaggagct atgggtcttg tcatgaaagg acaaggggtt caagctatct acggtccaaa
     5401 agctgacatt ttgaaatctg atatccaaga tatccttgat tcaggtgaaa tcattcctga
     5461 aactcttcca agccaaatga ctgaagcaca acaaaacact gttcacttca aagatcttac
     5521 tgaggaagtt tactcagtag cagacggtca agttgttggt ttggaacaag taaaggatcc
     5581 agtatttgct caaaaaatga tgggtgatgg atttgcagta gaacctgcaa atggaaacat
     5641 tgtatctcca gtttcaggta ctgtatcaag catcttccca acaaaacatg cttttggtat
     5701 tgtgacggaa gcaggtcttg aagtattggt tcacattggt ttggacacag taagtcttga
     5761 aggtaaacca tttacagttc atgttgctga aggacaaaaa gttgcagcag gagatctcct
     5821 tgtcacagct gacttggatg ctatccgtgc agcaggacgt gaaacttcaa cagtggttgt
     5881 cttcacaaat ggtgatgcaa ttaaatcagt taaattagaa aaaacaggtt ctcttgcagc
     5941 taaaacagca gttgctaaag tagaattgta atatacttga ggttggaagc tgtattccaa
     6001 cctcttattt tgggagaaaa gaatgaaatt tttaacactc aatactcaca gttggatgga
     6061 gaaagaagca gaggaaaaat tccagatttt gcttgaagat attcttgaaa aggactataa
     6121 tttgatttgt tttcaagaaa tcaatcagga gatgacctcg tcagaggtgg aggttaatga
     6181 cctttatcaa gctttgccag cagctgagtc tattcaccaa gaccattatg ttagactctt
     6241 ggttgaaaag ttgttagagc aagggaaaaa ttactactgg acctgggctt ataaccatat
     6301 cggctatgac cgctaccacg aaggtgtggc tatcttgtct aaaacaccta ttgaagccag
     6361 agaaattttg gtttcagatg tggatgatcc aacagactat catactcgcc gtgttgccct
     6421 agctgaaact gtagtcgatg gcaaggagct agcagttgcc agtgttcatc tctcttggtg
     6481 ggataaaggt ttccaagaag aatgggcacg atttgaggct gtcttgaaaa aattgaacaa
     6541 gccactttta ctagctggag attttaacaa cccagcaggt caggaaggtt accaagctat
     6601 tttagctagt ccattaggct tacaagacgc atttgaagtt gctcaagaga aaagtggtag
     6661 ctatactgtt ccgcctgaaa ttgatggctg gaaagggaac actgaacccc ttcgaatcga
     6721 ttatgtcttt actaccaaag agttagcggt ggaaaattta catgtcgtat ttgatggtaa
     6781 caagagtcca caagtgagtg atcactatgg cttgaatgct atgttaaact ggaaataata
     6841 actgaaaaga ggttggaact ataaaattcc agccttttct tactagagaa gctactggaa
     6901 atagcctaaa taagtgagac tactgtaatg gaataaaata tggtataatt gataagatag
     6961 atagaatcga ggatgttatg tcatttacga aatttcaatt taaaaactat attagagaag
     7021 ccttgaagga gttaaaattt acaactccaa cagaggtgca agacaagttg attcctattg
     7081 ttttggcagg tcgtgaccta gtaggagaat caaaaacagg ttcaggtaag actcatactt
     7141 tcttgttacc gattttccag caattagatg aagctagcga tagtgtacaa gcagtgatta
     7201 ctgcaccgag tcgtgagttg gctactcaaa tttaccaagt atcgcgtcag atttcagctc
     7261 actcagatgt cgaagttcgt gtggttaatt atgtgggtgg tacggataag gctcgccaga
     7321 ttgagaaatt ggaaagcaat cagcctcata ttgttattgg aacaccaggc cgtatctacg
     7381 acttggttaa atctggtgat ttagctattc ataaagccaa gacatttgtt gttgatgaag
     7441 cagacatgac cttggatatg ggattcttgg aaactgttga taagattgct ggctgtcttc
     7501 caaaagactt gcaattcatg gtcttctcag cgactatccc acaaaaactg caaccattct
     7561 tgaaaaaata cttatcaaat cctgttatgg agaaaattaa gaccaaaacg gttatttctg
     7621 acaccattga taattggttg atttcgacca agggacatga taagaatgct caaatttacc
     7681 agttgactca gttgatgcag ccgtatttgg caatgatttt tgttaacact aaaacgcgtg
     7741 ctgatgaatt gcattcatat ctgactgctc aaggcttgaa ggttgcaaaa atccatggcg
     7801 atattgcccc tcgtgaacgc aagcgaatca tgaatcaggt gaaaaatctg gattttgagt
     7861 atattgtcgc aacagatttg gcagcacgtg ggattgacat tgaaggtgtc agccatgtca
     7921 tcaatgatgc cattccgcaa gacttatctt tttttgttca ccgtgttggt cgtactggac
     7981 gaaatggcct accaggtaca gctattaccc tttatcagcc aagtgatgac tcggatatcc
     8041 gtgagttgga gaaattggga atcaagttta gtcctaagat ggtcaaagac ggggaatttc
     8101 aagataccta tgaccgtgat cgtcgtgcca accgtgagaa aaaacaagat aaacttgata
     8161 tcgaaatgat tggtttggtt aaaaagaaaa agaaaaaagt caaaccgggt tataagaaga
     8221 aaattcaatg ggcggttgat gaaaagcgcc gtaaaaccaa gcgtgctgaa aatcgcgctc
     8281 gcggtcgtgc agagcgtaaa gctaaacgcc aaacatttta atagaaattg ttggagtact
     8341 gagctccaac ttttttattt atgagaacga actatctaaa ccgaaacact acattaaaga
     8401 ctgcaaattg cgattaaaaa tggtataatg ataaagttat atagtcccga taagatggta
     8461 ggtatttatt acgaagagtt ttcctatcag tagtttgcac ttcgcaaaga atgacggctt
     8521 cgaggtcgct cgtctttcta cggttgccgc catactgcgg tcaccctatg cgccttacta
     8581 gcttcagaaa taaaaaagta tcgtttttat ttctgaagct agtaactctt agataatgga
     8641 aattcctatc agtactttgt aactctataa caatattttt taagggggga catttttatg
     8701 tcagaacgta aattattcac gtctgaatct gtatctgagg ggcatccgga taagattgca
     8761 gaccaaattt cagatgcgat tttggatgct attttagcaa aggatccaga ggcgcacgtt
     8821 gctgctgaaa cagctgtata tactggttct gtccacgttt ttggtgaaat ttctacaaat
     8881 gcctatgtgg atattaaccg tgtggttcgt gataccattg cagagattgg ttataccaat
     8941 acagaatatg gattttctgc tgagacggtg ggagtacacc catctttggt ggaacaatct
     9001 cctgacatcg ctcaaggtgt taacgaagcc ttggaggttc gtggaaatgc tgatcaagat
     9061 ccactggact tgattggagc aggtgaccaa gggctcatgt ttggatttgc agtagatgaa
     9121 acagaagagc tcatgccatt gccaattgca ctcagtcata aattggttcg tcgtctggca
     9181 gaacttcgta agtctggaga aattagctat ctccgtccag atgcaaaatc acaagttaca
     9241 gttgagtacg atgaaaatga ccgtccggta cgtgtagata cagtcgttat ttctactcag
     9301 catgatccag aggccactaa tgaacaaatc catcaagatg tgattaacaa ggtcatcaaa
     9361 gaagttattc catcttctta tcttgatgat aagacaaaat tctttatcaa tccgacaggt
     9421 cgttttgtaa tcggtggtcc tcaaggggac tcaggtttga ctggtcgtaa gattattgta
     9481 gatacttatg gtggctactc tcgtcatggt ggtggtgcct tctctggtaa agatgcgact
     9541 aaggtggatc gttcagcctc ttatgcggct cgctatattg ccaagaatat cgttgcagca
     9601 ggccttgcta agaaggcaga agtgcagttg gcctatgcta tcggtgttgc gcaacctgtt
     9661 tctgttcgta tcgatacttt cggtacagga acagtagctg aaagtcaact tgaaaaagcg
     9721 gctcgtcaaa tctttgacct tcgccctgca gggattatcc aaatgctgga cctcaagcgt
     9781 ccaatttacc gtcaaacatc ggcttacggt cacatgggac gtacagatat tgatcttcca
     9841 tgggaacgtt tggataaggt agatgctttg aaagaagcag taaaataaga ttttaagagg
     9901 ggaacgtcct ctctttttta tagtttttaa ctatactggg atactgttct gaaaatccat
     9961 tttgcgaaag tagagattta catgtatagt agattgaaac tagaatagta cacttcaact
    10021 tctaaaacat tgttagaaat cgatttgact gtcctgatcg atttctcctg ttcttgtttc
    10081 attttactat atttctttaa aaatgataaa ggttgagatt tctcctcgta atagataatc
    10141 atggggatat ttcaatccaa agttttattc gttatcactt gactattgca aggttttcta
    10201 gagcaacaga gtcatggaat ggactcatgg ttgagatttc tccttgttgc ttggacttca
    10261 ttcaaaagtc tgttacccaa gccttgttca aacttccaat acactagctg tttctatagc
    10321 atgacttctg tactagactt tcttttccaa ataaatagat agaaccacag aatctagtaa
    10381 acctagaatt aaaattatgg tataatatta gcaataaaag aaatctggag gattagaatc
    10441 atggtatcaa cgaaaacaca aattgctggt tttgagtttg acaattgctt gatgaatgca
    10501 gcaggtgtgg cttgtatgac gatagaggag ttagaagagg tcaaaaactc agcggcagga
    10561 acctttgtta ctaagacagc gaccttggac ttccgtcagg ggaatcctga gccacgctac
    10621 caagatgttc cacttggttc catcaactct atgggcttgc caaataatgg cttagactat
    10681 tatttggatt atcttttgga tttgcaggaa aaagagtcga accgaacttt cttcttatct
    10741 ctggtcggta tgtctccaga ggaaacccat actattttga aaaaagtcca agagagtgat
    10801 tttcgtggtc tgactgagct aaatctttcc tgtccaaatg ttccaggtaa acctcagatt
    10861 gcctatgatt ttgagacaac agaccggatt ttggcagaag tgtttgctta cttcaccaaa
    10921 cctcttggaa ttaaattgcc accttatttt gatattgttc actttgatca agcggcagct
    10981 attttcaaca aatatccgct caagtttgtc aactgcgtta actctatcgg aaacggcctc
    11041 tatatagaag acgaatctgt cgttattcgg cctaagaatg gttttggtgg aattggtgga
    11101 gaatacatca aaccgactgc tctagccaat gttcacgcct tttatcaacg tttaaatcct
    11161 caaatccaaa ttatcggaac aggtggcgtt ctgactggtc gagatgcctt tgaacacatc
    11221 ctctgtggag caagtatggt gcaggtggga acgacccttc acaaagaagg cgtcagtgct
    11281 tttgaccgca ttaccaatga actgaaagca atcatggtgg aaaaaggcta cgagagctta
    11341 gaagatttcc gtgggaaatt gcgctatatt gactaaatta aatcgaaaaa tctgaagaaa
    11401 ggagagacga tgctagccat tgaagaaagt cagaagttga ctttatcaaa tttaccgagc
    11461 ctgagcctat ttacagggac agatcagggt cagtttgaag tgatgaagag tcaaatgttg
    11521 aaacagattg ggtatgattc tgctgacctc aactttgcct actttgatat gaaagaagta
    11581 gtttacaagg atgtggaact ggagttggtc agccttcctt tctttgcgga tgagaaaatc
    11641 gtgatattag attattttat ggatatcacg actgctaaga aacgcttttt gacagatgat
    11701 gagcttaagt catttgagga ataccttgac aatccttctc caacaaccaa gttgataatc
    11761 tttgcagaag gaaagctgga tagcaaaaga cggttagtca aattacttaa gcgtgatgcc
    11821 aaggtcttcg atgcagtaga agtaaaagaa caagaattgc gccagtattt ccaaaagtgg
    11881 agtcagaaac aaggtctgca gtttaccaat cattcttttg aaaatctcct catcaagtcg
    11941 gggtttcaat ttagcgaaat ccagaaaaat cttctctttt tacagtccta taaggcgaat
    12001 tctgttattg aggaagagga tattgttaac gcaattccca agaccttgca ggacaatatt
    12061 tttgatttaa ctcagtttat tctgactaaa aagatggatc aggcgcgcga tttggtgaga
    12121 gacttgacct tgcaagggga agatgaaatc aaactgattg cagtcatgct gggacaattt
    12181 cggactttta ctcaggtgaa gattttggcg gagtctggcc aaacagaatc acagattgca
    12241 agtagtttag gtagttatct gggacgtaac ccaaatcctt atcaaatcaa gtttgcatta
    12301 agagattcga gaggactttc tttgagcttt ctgaagcaag ctatttccta tttgattgag
    12361 acagactatc agattaagac aggtctttat gaaaaaggtt tcctttttga aaaggcactc
    12421 ttacagattg ctagtcaggt caattgacat ttgttgaaac tacaacccgc gtcaggatta
    12481 tcttgtcgtg ggtttcttgc tgatttgctt attttattag aaagtatgtc agtgatttcc
    12541 ttatctataa gcaaaaaact tgaaaaaagt gaatgtttgc gttatcattt tcatatagaa
    12601 agaaaaagag gtattacaga tggctattat cttaccagaa cttccatatg catacgacgc
    12661 tttggaacca tacatcgatg cggaaacaat gcatttgcac catgacaaac accatcaaac
    12721 ttatgtcaac aatgccaatg cagctttaga aaaacaccct gaaatcggtg aagaccttga
    12781 agccttgctt gctgatatag aatctatccc agctgatatc cgtcaagcac ttatcaacaa
    12841 tggtggcgga cacttgaacc acgctctttt ctgggaattg atgactcccg agaaaacagc
    12901 tccttcagca gaactggcag cagcaatcga tgcaacattt ggttcatttg aagaattcca
    12961 agcagccttc actgcagcag caacaactcg ttttggttca ggttgggcat ggttggttgt
    13021 caacaaagaa gggaaacttg aagtgacttc aacagcaaac caagacacac caatctcaga
    13081 aggtaaaaaa ccaatcttgg gcttggacgt ttgggaacat gcttactacg tgaaataccg
    13141 caacgtgcgt cctgactata tcaaagcttt cttttcagta atcaactgga ataaagtaga
    13201 tgaattgtac gcagctgcta aataatgata gttggaggga agaattgttc ttctcttttt
    13261 taggttataa gattctggtc tgacaaaatc gtcagacttt tttcattttt atgagaaacg
    13321 tgctaactgc tagaaattat ggtagaataa agtattaagt aatcgtggaa aaaggatatc
    13381 tatgcgaaaa gaaattgcac ctgaattata caactataac aagtttcctg gtccggagtt
    13441 ccatttacac ggggacaagg tcgaaacgga agggatagct ttttccttgg ttgaaaatat
    13501 caaggatgcc tttgatgtga cgacttttaa tcagcgtttt tcagaagtat taaccaagtt
    13561 tgattatatc gtgggggact ggagcaacga acagcttcgc ctacgaggtt tttacaagga
    13621 tgaccgaaca gaagaaaaac ttgaaaaaat cagttgttta caagactacc ttttagagta
    13681 ttgtagttat ggttgtgcct attttgtctt agaaaatgaa gcccctaagc gagcatcatt
    13741 tgacaagaaa atgcgtaaga cggaagaaga aacaccttct agaaaaggaa agaaaccgac
    13801 tcaaactaaa cgaaagtcga atgcagataa gaaaaataga cgtcgtcaga aagaccagca
    13861 ttctcagaaa gaggacaagg gacaacgtca ttttgtcatt cgtcagaagt gagtagagaa
    13921 taaggagaag aaatgaaacc ctcaattcat agtttgacac gtcaaaccat gcaggaatgg
    13981 gtattggagc agggagaaaa gaaattccgt gcagatcaaa tctgggaatg gctctaccgt
    14041 aaacgtgtgc agtcatttga agaaatgacc aacctttcca aggatttgat tgctaagctc
    14101 aatgaccagt ttgtggtcaa tcccttgaaa caacgtattg tgcaagagtc tgctgatggt
    14161 acagtcaaat atctctttga attgcctgac ggtatgttaa ttgaaactgt actcatgcgt
    14221 caacactatg gtttatctgt ctgtgtgacc actcaggtcg actgtaatat cggttgtacc
    14281 ttctgtgcct ctggtttgat taagaagcaa cgtgacctca ataacggtga aatcgtagcg
    14341 caaatcatgc tggttcagaa atactttgct gagcgtggtc aggatgaacg cgtcagccat
    14401 atcgttgtca tgggaatcgg tgagcccttt gataattaca acaatgtctt gaatttcgtt
    14461 tgtactatca atgatgataa aggaatggct atcggtgctc gtcacatcac ggtttcaacc
    14521 tcagttttgg ctcataaaat tcgtaacttt gctgatgaag gggttcaggt caatcttgcc
    14581 gtgtctcttc acgcacccaa caatgaactt cgttcaagta tcatgaagat taaccgtgcc
    14641 tttccgattg aaaaactctt tgctgctatt gaatactaca ttgagacaac aaaccgtcgt
    14701 gtaacctttg aatatatcat gcttaatgaa gtcaacgacg gtgtagaaca agccttggaa
    14761 tttgctgaat tgctcaagaa catcaagaaa ttgtcttatg taaacttgat tccttataac
    14821 ccagttagtg agcatgacca atatagccgt agtcccaaag agcgcgtgtt ggctttctat
    14881 gatacgctta agaaaaaagg ggtcaactgt gtggttcgtc aagagtatgg tacagatatt
    14941 gacgcagctt gtggacaatt gcgctctaac acaatgaaac gtgaccgcca gaaggcagtc
    15001 gcagaggtaa atccgtaaaa tgactaaaaa aagagaatta atcttaagat tgggagtggc
    15061 tgtttatagc ctatgcattg tctatttttg ctttactcct caagctaccg tggaagtggg
    15121 aactccaggt attcagcatc ttggacgcct ggtttttctt ttgactcctt tcaattctct
    15181 ctggaaactg ggcgaagtga gtgacattgg acaattatgt tggatttttt tacaaaatat
    15241 cctcaatgtc ttcttgtttt ttcctctgat tttccaactc ctttatctat ttccaaattt
    15301 gcggaaaaca aaaaaggttc ttctttttag ttttcttgtg agtcttggaa tcgagtgtac
    15361 gcaattaatc ttggactttt tctttgattt caatcgcgtc tttgagattg atgatttgtg
    15421 gaccaacact ttgggtggct atctggcttg gctcctttat aaacgattac ataaaaacaa
    15481 gataaggaat taaaatgagt attttagaag ttaaaaatct gagtcacggt tttggtgacc
    15541 gtgcaatttt tgaagatgtg tccttccgtc tcctcaaggg agaacatatc ggcctggtcg
    15601 gtgccaatgg tgaaggaaaa tcaaccttta tgagtatcgt gactggtaaa atgctgccag
    15661 atgaaggaaa ggttgagtgg tccaaatatg tgacggctgg ttacttggat cagcactctg
    15721 tccttgctga aagacagtcg gtgcgtgatg ttctccgtac ggcttttgat gagcttttca
    15781 aagctgaagc tcgtatcaat gacctctata tgaaaatggc tgaagacggc gcggatgttg
    15841 atgctctcat ggaagaagta ggagaacttc aagaccgtct ggagagtcgt gatttctata
    15901 ccttggatgc taagattgac gaagtagcgc gtgctcttgg tgttatggac tttggcatgg
    15961 atacggatgt aacttctttg tcaggtgggc aaagaaccaa ggtgcttttg gcaaaacttc
    16021 tccttgaaaa gcctgatatt ttgctgttgg acgagccgac caactacttg gatgctgagc
    16081 atattgattg gctcaagcgc tatctccaaa actatgagaa tgcctttgtt ctcatttcgc
    16141 acgatattcc attcctcaat gacgttatca atattgtcta tcatgtggaa aatcaacagc
    16201 tgacgcgtta ctctggtgac tactaccagt tccaagaagt ttatgctatg aagaaatctc
    16261 agctagaggc agcctacgaa cgccagcaga aagagattgc agacctcaag gactttgtgg
    16321 ctcgtaataa agcccgtgtt gcaactcgta atatggctat gtctcgtcaa aagaaattgg
    16381 ataagatgga tattatcgaa ctccaaagtg agaaaccaaa accatccttt gatttcaaac
    16441 cagctcgtac accagggcgc tttatcttcc aagccaagaa cttgcaaatt ggttacgacc
    16501 gtcctcttac taagccttta aattttacct tcgaacgcaa tcaaaaggtt gcgattattg
    16561 gtgctaatgg tattggaaaa acaactctct tgaagagtct cttgggcatt atctcgccaa
    16621 tcgctgggga agtggagcgt ggagattatt tagaacttgg ttattttgag caggaagtag
    16681 aaggcggtaa tcgccaaact cctcttgaag ctgtctggaa tgcctttcct gcccttaatc
    16741 aagcagaagt ccgtgcagcc cttgcccgtt gtggtttgac aaccaaacat attgaaagcc
    16801 agattcaagt attatcaggg ggagagcaag ccaaggttcg tttctgtctc ttgatgaatc
    16861 gtgaaaacaa cgttttagtg ctggacgagc cgaccaacca tttggatgtg gatgcaaagg
    16921 atgagctcaa acgcgctctc aaagaatata ggggatctat ccttatggtc tgccacgagc
    16981 cagactttta tgaaggctgg atagaccaaa tatgggattt taataattta acttaagaat
    17041 gagagtaaaa gaaatacagt accgaatgtg gatactgtat ttttttggtt tttaagattt
    17101 aaaatcgtag tctttcacca cttcgatgct gtcgattgtg atagcagtag ttggcttgtc
    17161 tttttcatct ttttcggctt tagcaatctt atccacaaca tccataccgt caatcacttg
    17221 accaaagact gggtgtttgc catctagact agggtttcca ccttctttgt aggcttcgat
    17281 aattttctgt ggatacttgc ttgtagggag tttagaagag gtatctgtag agttttggtt
    17341 gatgaagaac tggctgccat tggtgtttgg ttgaccagta ttagccatag caagagcacc
    17401 gcggatgtta tacaaataag gagtaatctc gttcttgaaa ccagttcctt tgtctttagt
    17461 cttatccttg tcatgccaga tggactgacc acctgtaccg tccccttttg gatctccagt
    17521 ttggaccata aagccatcga tgacacggtg gaaggtaata ccgttatagt agccttcttt
    17581 ggcgtgagtg aggaaatttt caaccgctag aggagcgagt ttagggaaga gtttaatgcg
    17641 aatatcacct tggcttgtgt ggagaatcac ttcggcttca tcttcagcaa cttccttaga
    17701 tagttgtggg aaattggcgt tttcgtttgt taaagcatcg tttaactcct tggcagattg
    17761 ggcagctact ttggaacttt cttcagcagc aagactggaa tcaacataat catcaccacg
    17821 cagactgcgt tggacgctgc tacacccagc tagggctaca gtagacagta aaagaagggt
    17881 tgctagtttt ttcattctgt tcctctcaaa aattcattta taccattgta ccctaaaagg
    17941 aagtggattt caaatattag tagtttacag aactggctta gataatagtt gggatttcat
    18001 catcatcttg aaaatcttca acttcttccc cagtaaatgg tttctgaaaa gaacttagta
    18061 taaaacgggc aattggttca acaattagga aatttagagg aagagctgca ataaagttta
    18121 cgaaccaggc gcttagatag gtattgggtg tgatagcaat gccattgtaa aaaagtccat
    18181 agaaggacat gaaagtgacc agctaattct tcttctgaaa aatgctcatg aagggcttga
    18241 tccaggtcgc aagatttcat tttagaaagt ccagtaactc ccttcttctt caggatattc
    18301 tttttcatgg tattatttag atggtcaagt gagtcaaaag aagtttcgac aagagcatac
    18361 cctttttcta ccagagtttt tagatgttta ggggcatcaa tgccataagt gtactcaaag
    18421 tatttaggga accagatctt aagaaagctc gtaaagctag ccagttctct aaacgttaag
    18481 aactagctac aacacagcat ttacaacact tcatggttca cactatgggg tgttttttta
    18541 tgattttaag caaaattcac acttaattga aagttattta aattagatga aaatttattt
    18601 tttgaaaaag tgggaaaaca ttgattttaa gcgaattttg aatcatattc gaatgggagt
    18661 ttttaaaatc atgtttcagt ggtaaaagta ccaaactgga ttctccatag taaaatgata
    18721 tctcctgcta gtgaatcatc tgctaataag tccatatttc gcttgggaac gggcttggga
    18781 cttaggagcc aagtatccaa atctcgttca ggcgatatga agggtttgac ttggtgttct
    18841 ttataaaatt tttctaaaat agtattcacg gatagcatct ttcaaacatt tatttcctat
    18901 tctaacaaat ttctaaatac tttaagaaat ttatagtctg tcaagttttt ttcttgacac
    18961 ctgccagaaa tctgctataa tagaacatgt gctaaatagc tcagctattt caccgaaata
    19021 aaaaattaaa gaaatgagac tttaaaaatg gcagttaaaa tccgtttgac tcgtatgggt
    19081 tctaagaaaa aacctttcta ccgtatcaac gtagcagatt cacgttcacc acgtgacgga
    19141 cgtttcatcg aaacagttgg aacttacaac ccacttgttg ctgaaaacca agtaactttg
    19201 aaagaagacc gcgttcttgc atggttggct aatggagctc aaccttcaga cacagtacgt
    19261 aacatccttt caaaagaagg cgtattgaaa aaattccacg attctaaatt ctcaaaataa
    19321 gtttaaagta ggttgacaga tggatacgat tgaaaatctc attattgcga ttgtgaaacc
    19381 cttaatttca caaccagatg ccttaactat caagattgag gatacaccag aatttttgga
    19441 atatcatttg aatcttgatc aaagcgatgt gggtcgtgta atcggtcgta agggtcgcac
    19501 tatttctgcg ataagaacga ttgtctactc tgtcccaact gaatacaaaa aagtaagaat
    19561 cgttattgac gaaaaataaa aagggcggga cggatgtctc gcttttttgt gtggaataga
    19621 agatataata aaatgaaata ataacaggac aaatcgatca ggacagtcaa atcgatttct
    19681 aacaatgttt tagaagtaaa ggtgtactat tctagtttca atatactata aaagattgaa
    19741 cgtttaggca attccaagac tacttactcg aacattaaca gcagtctcgg actgatgaag
    19801 gtctctttat gaagttagtg gaggaagtcg gagaagtagc cgaggttttg aataggcgct
    19861 ctggtcgaaa agaagggata ggattcgaac gaggaacttg ccagagaact ggctgatatc
    19921 attcactaca ccgtcgctat tgctgctatc aagcatattg acctaactag aaccatcttt
    19981 gagaaagaca aaactgcagc cgttaattac caacataaac atgatttgga aggattcttg
    20041 aagggaaagt agagatgttg tccccttcct ccaacttgag aaaatccttg gaacgtgata
    20101 aaataaaaga taaagatttt taataattca ttatatagaa tgagtggatt tctttatgaa
    20161 gaggtttgaa gtatctacag aaattggtag tctctctgtt acttatcaaa agcaaaagaa
    20221 agtgctagtt tgtctaaacg gagcaggttt gctaccaagt tatgaaaatt tttcacttat
    20281 acttgaaaaa cctcctccta caattggtta tttgacaatt gattttccga acacaggtag
    20341 gagcccgatt catgaccaag ctggaaaaaa tctagataat cttgcagatg cggtttatga
    20401 agtacttgaa gaattaggga tttctgaata tatactttgt gcacatagtt ggagtggaat
    20461 tttagcttgc aaattactcg agaaaccaat taagtgtcag gctttagtag caattgaacc
    20521 gacaactaaa aaagtcatgt ttgctgattt ttcagaaaat ccttatccag aaatggaaga
    20581 gcagatgagg ctgattgacg agtatggacc tgaactttat tttaagaact taactcaagc
    20641 aacatttagt cctgaaacga ataaagaaat ctgggaatta atgcaagaaa aaggcttaga
    20701 gttggaaaat caagatccag aatttcagat atctggagag attactgagg aagattttga
    20761 gaatttgtcg atagaatctc atgtccctgt atttattttt tgtcagactt atagagaaaa
    20821 agagtacaga gaatcagaat attggacttc caatactaaa ctcattttag gagggaatca
    20881 ccattattta caatggtcag aatcggaaaa aattgcggct attattcgag aattgtcaga
    20941 ataagatgga aaaaaggaga ttacaggaga caagatgaac tactttaatg ttgggaaaat
    21001 cgttaatacg cagggattac agggtgagat gcgagtcttg tctgtgacgg attttgcaga
    21061 agaacggttt aaaaaaggag ctgagctggc tttgtttgat gaaaaagatc agtttgtcca
    21121 aacagtgacc atcgctagcc accgtaaaca gaagaacttt gacattatta aattcaaaga
    21181 tatgtaccat atcaatacta tcgaaaagta caagggatac agtctcaagg tcgctgagga
    21241 agatttgaat gacctagacg atggtgaatt ttactatcac gagattatcg gtttggaagt
    21301 ctatgagggt gatagcttgg ttggaaccat caaggaaatc ctgcaaccag gtgctaatga
    21361 tgtctgggtg gtcaaacgaa aaggcaaacg tgatttgctt ttaccttata tcccaccagt
    21421 ggttctcaat gttgatattc caagtaaacg ggtcgatgtg gaaatcttag aagggttaga
    21481 cgatgaagat tgatatttta accctctttc cagagatgtt ttctccactg gagcactcaa
    21541 tcgttggaaa ggctcgagaa aaagggctct tggatatcca gtatcataat tttcgagaaa
    21601 atgctgaaaa ggcccgtcat gtagatgatg agccctacgg aggcggtcag ggcatgttgc
    21661 tcagagcaca acctattttc gattcctttg atgctattga aaagaaaaat ccgcgcgtta
    21721 ttctccttga tcctgctgga aagcagtttg atcaggctta tgctgaagat ttggctcaag
    21781 aggaagagct aatctttatc tgtgggcact atgagggtta tgatgagcgc attaagacct
    21841 tggtaacaga tgagatttcc ctaggcgact atgtcctcac tggtggagaa ttggcagcta
    21901 tgaccatgat tgatgctaca gttcgcctga ttccagaagt gattggcaag gagtctagcc
    21961 accaagatga tagtttttct tcaggtcttt tagaatatcc tcagtacaca cgtccctatg
    22021 attatcgagg catggtcgtg ccagatgtat tgatgagtgg ccaccatgaa aagattcgtc
    22081 agtggcgatt gtacgagagt ttaaagaaaa cctacgagcg cagaccggat ttacttgaac
    22141 attatcaact gacagtagaa gaagaaaaaa tgctggcaga aatcaaagaa aacaaagaat
    22201 aaaggagaaa cctatgcaag taatcaaacg taatggcgaa attgctgaat ttaatccaga
    22261 taagatttac caagccatct tgaaggcagc ccaaactgtt tatgttttga cagatgattt
    22321 gcgtcaaaat cttgctcaag tcactaagaa ggtagttttg gatttacaag aagccaaggt
    22381 ggaacgtgcg actatcagta tgattcaatc tatggttgaa catcgtttat tgggcgcagg
    22441 ttacattacc attgcagaac actacatttc ctatcgttta caacgtgact tggaaagaag
    22501 tggttatgga gatcatattg cagttcattt acattttgaa caaattcgct aagaaaaaag
    22561 agtgggatga agcaactaca tctcactctt tcttaaatct atttttttgg gctgtttgga
    22621 cagttgaggg gacaaatcgc aggcgttgaa tatcgcttat acggataatc cggctcacat
    22681 ttttggcaaa atttttcacg ataatttgct ggccttgctg atcatatttg atgatatcac
    22741 ctgtaaaact tgtctcagac aagataaggt gaacagcggt ttgtttctgg atagcctctt
    22801 caatagtggc tgtaagggag ttggtttcag tctggtcagg ttggtcatgt ccatttaaaa
    22861 aatcatgtat tttatctaga acttgctgga atttatgtct catattggcc tcccttcttt
    22921 tatatcaata ttatataaaa ttttcctaaa atctacaaga ttttacgaat aaactaatga
    22981 aactaaaaaa ggagaagaaa atggcagaat ttacatttga aatcgaagag cacttgttga
    23041 ctctttctga aaacgaaaaa ggttggacca aggaaattaa ccgtgtgagc tttaatggtg
    23101 cccctgcaaa gtttgatatt cgtgcttgga gtccagacca tactaaaatg ggcaaaggga
    23161 ttactctctc aaatgaagaa tttcaaacga tggtggatgc ctttaaaggc aactaatact
    23221 cttcgaaaat ctcttcaaac cgcatcagct ttgccttgcc gtatgtatgg ttactgactt
    23281 cgtcagtttc atccacaacc tcaaaacagt gttttgagca tcctgcggct agcttcctag
    23341 tttgctcttt gattttcatt gagtataagt tttcaaaata aaaaaaagga tattgagtca
    23401 tgataactcg gtatcctttt ttagttagta aagtaagcct gatttttaag gcgttgaagt
    23461 aggggacggc taataaaact aatagccaga agtttgccaa ggtctggaat gataaaggga
    23521 agaaccccca caacaagagc tttttcaaat gccattccag ctaggaaatg caagctgaga
    23581 atcccgccga caaagacaag ggcatcaccc aagaggtttg caagaaaaat cttaacaaca
    23641 ccactcttgc tgttggttag agaggaagta agtccagagt aaacgagata aaaccaaaga
    23701 tagcctgcag tagggccaac taaagcctga aaaccagctc cacctcctgc aaagacagga
    23761 agaccgatag cacctagaag aagatagagt ccagcagaaa gtacagcctc tctcggtcta
    23821 aagacagtag caatcaagcc gattgcaaag ttttgcagag tgaagggaac aggtccaatt
    23881 ggaagactga tttgtgccaa aacagcaatg agagcagccc caatagcagg gatagcataa
    23941 acgtgagctt ttttcaaaat agttaacctc ttttctaatg tatagtaact attatactca
    24001 tataggagtc gatgtcaacc attttttaaa aacaaggtaa caaaaactgt aacagccgat
    24061 tttcgtgact agtcaagttg gataattttt ttgttgattc gaaaattgtc tgtataga
//
LOCUS       DAVKJD010000021        23315 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKJD010000021 DAVKJD010000000
VERSION     DAVKJD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23315
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.021"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            650..2803
                     /locus_tag="VW157_000928"
     CDS             650..2803
                     /locus_tag="VW157_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9552251.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKFTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2816..4165
                     /locus_tag="VW157_000929"
     CDS             2816..4165
                     /locus_tag="VW157_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9552252.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQGGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     TLLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4335..5042
                     /locus_tag="VW157_000930"
     CDS             4335..5042
                     /locus_tag="VW157_000930"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9552253.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQVAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5244..8969
                     /locus_tag="VW157_000931"
     CDS             5244..8969
                     /locus_tag="VW157_000931"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9552254.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFTQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLILGICNGFQALVK
                     SGLLPYGNFEAANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9062..10504
                     /locus_tag="VW157_000932"
     CDS             9062..10504
                     /locus_tag="VW157_000932"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9552255.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSCRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10541..11563
                     /locus_tag="VW157_000933"
     CDS             10541..11563
                     /locus_tag="VW157_000933"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9552256.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11560..12105
                     /gene="purN"
                     /locus_tag="VW157_000934"
     CDS             11560..12105
                     /gene="purN"
                     /locus_tag="VW157_000934"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9552257.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12189..12698
                     /locus_tag="VW157_000935"
     CDS             12189..12698
                     /locus_tag="VW157_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9552258.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12723..14270
                     /gene="purH"
                     /locus_tag="VW157_000936"
     CDS             12723..14270
                     /gene="purH"
                     /locus_tag="VW157_000936"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9552259.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDFDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14392..15654
                     /gene="purD"
                     /locus_tag="VW157_000937"
     CDS             14392..15654
                     /gene="purD"
                     /locus_tag="VW157_000937"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9552260.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            16057..16545
                     /gene="purE"
                     /locus_tag="VW157_000938"
     CDS             16057..16545
                     /gene="purE"
                     /locus_tag="VW157_000938"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9552261.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16532..17623
                     /gene="purK"
                     /locus_tag="VW157_000939"
     CDS             16532..17623
                     /gene="purK"
                     /locus_tag="VW157_000939"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9552262.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEVD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVAENPSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17633..17860
                     /locus_tag="VW157_000940"
     CDS             17633..17860
                     /locus_tag="VW157_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU9552263.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTGLNTLDHYPSVFIGKEEFE"
     gene            17923..19221
                     /gene="purB"
                     /locus_tag="VW157_000941"
     CDS             17923..19221
                     /gene="purB"
                     /locus_tag="VW157_000941"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9552264.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19276..23214)
                     /gene="strH"
                     /locus_tag="VW157_000942"
                     /pseudo
     CDS             complement(19276..23214)
                     /gene="strH"
                     /locus_tag="VW157_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
BASE COUNT         6349 a         4666 c         5577 g         6723 t
ORIGIN      
        1 ttaattagaa aaaaattttt aaagtcttcg gagaagaaaa cagaaaaatc tgttaaaaaa
       61 taatcaaaac tataaatgat gaatctgaat caaaattatt ttgcgcatgt aaagaggagt
      121 cttatagtaa cgagtcaaaa aaggagtaac tatgaatcgt aatttagaac ggtgttatct
      181 attctgacta ggaatagatc ataccagagg tagcttagaa atagcagaga cattagaaat
      241 tgaagtaata aataggatgt cgtaagtgtt actatcaatg atttatttgt ttcaagcttg
      301 cctagggtga cagtaaaaaa tcaatttcct ttcaatagca tatttttagt gggcaggact
      361 cttgttctgc ctattttttt atccaaaaag tgcagttggg agggagatag gctcatttgg
      421 gaaggaagtc cagtttttgt ttagtgattg gggtaagata gttgttatca gatgagttta
      481 tactcttcga aaatcaaatt caaaccacgt caacgtcgcc ttgccgtata tatgtgactg
      541 acttcgtcag tcctatctac aacctcaaaa cagtgttttg agcagcctgc ggctagtttc
      601 ctagtttgct ctttgatttt cattgagtat tagggaaaag gagatgaata tgaaatttgg
      661 gaaacgtcac tatcgtccgc aagtggatca gatggactgc ggtgtagctt cattagccat
      721 ggtttttggc tactatggta gttattattt tttggctcac ttgcgagaat tggctaagac
      781 gaccatggat gggacgacgg ctttgggctt ggtcaaggtg gcagaggaga ttggttttga
      841 gacgcgagcc attaaggcgg atatgacgct ttttgacttg ccggatttga cttttccttt
      901 tgttgcccat gtgcttaagg aagggaaatt gctccactac tatgtggtga ctgggcagga
      961 taaggatagc attcatattg ccgatccaga tcccggggtg aagttcacta aactgccacg
     1021 tgagcgtttt gaggaagaat ggacaggagt gactcttttt atggcaccta gtccagacta
     1081 taagccttat aaggaacaaa aaaatggtct gctctctttt atccctatat tagtgaagca
     1141 gcgtggcttg attgccaata tcgttttggc aacactcttg gtaaccggga ttaacattgt
     1201 gggttcttat tatctgcagt ctatcattga tacctatgtg ccagatcaga tgcgttcgac
     1261 actagggatt atttctattg ggctagtcat cgtctacatc ctccagcaaa tcttgtctta
     1321 cgctcaggag tatctcttgc ttgttttggg gcaacgcttg tcgattgacg tgattttgtc
     1381 ctatatcaag catgtttttc acctccctat gtccttcttt gcgacacgca ggacagggga
     1441 gatcgtgtct cgttttacag atgctaacag tatcatcgat gcgctggctt cgaccatcct
     1501 ttcgattttc ctagatgtgt caacggttgt cattatttcc cttgttctat tttcacaaaa
     1561 taccaatctc tttttcatga ctttattggc gcttcctatc tacacagtga ttatctttgc
     1621 ctttatgaag ccgtttgaaa agatgaatcg ggataccatg gaagccaatg cggttctgtc
     1681 ttcttctatc attgaggaca tcaacggtat tgagactatc aagtccttga ccagtgaaag
     1741 tcagcgttac caaaaaattg acaaggaatt tgtggattat ctgaagaaat cctttaccta
     1801 tagtcgagca gagagtcagc aaaaggctct gaaaaaggtt gcccatctct tgcttaatgt
     1861 cggcattctc tggatggggg ctgttctggt catggatggc aagatgagtt tggggcagtt
     1921 gattacctat aataccttgc tggtttactt taccaatcct ttggaaaata tcatcaatct
     1981 gcaaaccaag cttcagacag cgcaggttgc caataaccgt ctaaatgaag tgtatctagt
     2041 agcttctgag tttgaggaaa agaaaacagt tgaggatttg agcttgatga agggagatat
     2101 gaccttcaag caggttcatt acaagtatgg ctatggtcga gacgtcttgt cggatatcaa
     2161 tttaaccgtt ccccaagggt ctaaggtggc ttttgtgggg atttcagggt caggtaagac
     2221 gactttggcc aagatgatgg ttaattttta cgacccaagt caaggggaga ttagtctggg
     2281 tagtgtcaat ctcaatcaga ttgataaaaa agccctgcgc cagtacatca actatctgcc
     2341 tcaacagccc tatgtcttta acggaacgat tttggagaat cttcttttgg gagccaagga
     2401 ggggacgaca caggaagata tcttacgggc ggtcgaattg gcagagattc gagaggatat
     2461 cgagcgcatg ccactgaatt accagacaga attgacttcg gatggggcag gaatttcagg
     2521 tggtcaacgt cagagaatcg ctttggcgcg tgctctcttg acagatgcgc cggtcttgat
     2581 tttggatgag gcgactagca gtttggatat tttgacagag aagcggattg tcgataatct
     2641 catggctttg gacaagacct tgattttcat tgctcaccgc ttgactattg ctgagcggac
     2701 agagaaggta gttgtcttgg atcagggcaa gattgtcgaa gaaggaaagc atgctgattt
     2761 gcttgcacag ggtggctttt acgcccattt ggtcaatagc tagaaagagg agaggatgaa
     2821 accagaattt ttagaaagtg cggagtttta taatcgtcgt taccataatt tttccagtag
     2881 tgtgattgta cccatggccc ttctgctcgt gtttttactt ggctttgcaa ctgttgcaga
     2941 gaaggagatg agtttgtcca ctagagctac tgtcgaacct agtcgtatcc ttgcaaatat
     3001 ccagtcaact agcaacaatc gtattcttgt caatcatttg gaagaaaata agctggttaa
     3061 gaagggggat cttttggttc aataccaaga aggggcagag ggtgtccaag cggagtccta
     3121 tgccagtcag ttggacatgc taaaggatca aaaaaagcaa ttggagtatc tgcaaaagag
     3181 cctgcaagga ggggagaacc actttccaga ggaggataag tttggctacc aagccacctt
     3241 tcgcgactac atcagtcaag caggcagtct tagggctagt acatcgcaac aaaatgagac
     3301 catcgcgtcc cagaatgcag cagctagcca aacccaagcc gaaatcggca acctcatcag
     3361 tcaaacagag gctaaaattc gcgattacca gacagctaag tcagctattg aaacaggtgc
     3421 ttccttggcc ggtcagaatc tagcctactc tctttaccag tcctacaagt ctcagggtga
     3481 ggaaaatccc caaactaagg ttcaggcagt tgcacaggtt gaagcacaga tttctcagtt
     3541 agaatctagt cttgctactt accgtgtcca gtatgcaggt tcaggtaccc agcaagccta
     3601 tgcgtcaggg ttaagcagtc aattggaatc ccttaaatcc caacacttgg caaaggttgg
     3661 tcaggaattg acccttctag cccagaaaat cttggaggca gagtcaggta agaaggtaca
     3721 gggaaatctt ttagacaagg ggaaaattac ggcgagtgag gatggggtgc ttcatcttaa
     3781 tcctgagacc agtgattcta gcatggttgc agaaggtacc ctactagccc aactttatcc
     3841 atctttggaa agagaaggga aagccaaact cacagcttat ctaagttcaa aagatgtagc
     3901 aagaatcaag gtcggtgatt ctgttcgcta tactacgact catgatgccg ggaatcaact
     3961 tttcctagat tctactatta caagtattga tgcgacagct actaagactg agaaagggaa
     4021 tttctttaaa atcgaggcgg agactaatct aacttcggag caggctgaaa aacttaggta
     4081 cggggtggaa ggccgcttgc agatgattac gggcaagaaa agttacctac gttattattt
     4141 agatcaattt ttgaacaaag agtaatgttc gtgtttttag agttaaataa tttttaaact
     4201 gtgagaaaga ttcttcttgc agttttttct ttacaatttt tgaaaaacat ctgccattta
     4261 ttcggttaaa ttcttgtgtt ttttggtttt ttgtggtaaa atgtgctcaa gtaatacgaa
     4321 aggcgaactt taaaatgtca aaacaattga tctattcggg aaaagctaaa gatatctata
     4381 caactgagga tgaaaatctt attatttcaa cttacaagga ccaggcgact gctttcaacg
     4441 gtgtcaagaa ggagcaggtt gcaggtaagg gagtcttgaa taatcagatc tcatctttta
     4501 tttttgagaa attaaatgcg gctggtgtgg cgactcactt tgtggagaaa ctttcagaca
     4561 cggaacaact caataaaaaa gttaagatta ttcctttgga agtcgtgctc cgcaactata
     4621 ctgctggttc cttttcaaaa cgttttggtg tggatgaggg aatcgccttg gagactccga
     4681 ttgtcgaatt ttactacaaa aatgatgatt tggatgatcc atttatcaat gatgaacatg
     4741 tgaaattcct acagattgcg gatgaccagc agattgccta cttgaaggaa gaaacgcgtc
     4801 gtatcaatga actattgaaa gtctggtttg ctgagattgg gcttaaattg attgacttta
     4861 agctagagtt cggttttgac aaggatggca agattatctt ggcagacgaa ttttcaccag
     4921 ataactgccg cttgtgggat gctgatggca accacatgga taaggatgtt ttccgtagag
     4981 gattgggaga actaaccgac gtttatgaga ttgtttggga aaagttgcag gaattgaaat
     5041 aatctgtttg caatggaaaa ccttcgtctc tcaactaaaa ggactcaggc tgaaaaggtc
     5101 ccccagacct tttcactctg tagagaacta ggtgaactaa ccgatgttta cgaaattgtc
     5161 tgggaaaagt tgcagggttt aaaataacaa cctcaaggct gtttgggaat attgcaagag
     5221 ctgaaataaa ggaataagaa ttgatggata aacgtatttt tgttgaaaaa aaggctgatt
     5281 ttcaggtcaa gtcagagagt ttggttagag agctccagca caacttggga ctgtcaagct
     5341 tgaaaagtat tcgtattgtg caagtatatg atgtatttga cttggctgag gacttgtttg
     5401 cacctgcaga gaagcacatt ttctctgagc aggtaaccga ccatgtttta gatgaagtat
     5461 ctgtgcaggc ggatcttgct aactatgctt tctttgccat tgaaagtctg ccagggcagt
     5521 ttgaccagcg tgcagcttcg tcacaggaag ccttgctttt gttgggaagt tcgagtgacg
     5581 tgacagtcaa cacagcccaa ctttacttgg tgaataaaga tattgatgcg actgagttgg
     5641 aagctgtcaa aaactacctg ctcaatccag ttgattctcg tttcaaggat atcacgatag
     5701 ggattgccaa gcaggagttt tcagagtcag acaagaccat tccaaaattg cctttctttg
     5761 aaagctatac agcagaagac tttgctcgct acaaggccga acaagggatg gccatggaag
     5821 tggatgattt gctctttatc caagactact ttaagtcaat cgggcgcgtg ccaactgaga
     5881 ctgaactcaa ggttttggac acttactggt ctgaccactg ccgtcatacg acttttgaga
     5941 cagagttgaa acacatcgac ttttcagctt ctaaatttca aaagcaattg cagtcaacct
     6001 atgacaagta tattgccatg cgcgaggaat taggtcggtc tgaaaaacca caaaccttga
     6061 tggatatggc gactattttc ggtcgttatg agcgtgctaa tggacgattg gatgatatgg
     6121 aagtctctga cgaaatcaat gcctgctcag ttgaaattga agtggacgtt gatggtgtca
     6181 aggaaccttg gctcctcatg tttaaaaacg aaacccacaa ccatccaaca gaaattgagc
     6241 catttggtgg agcggctacc tgtatcggtg gagctattcg tgatccgttg tcaggccgtt
     6301 cctatgttta ccaagccatg cgtatttcag gtgctggtga tattacagca ccgatttcgg
     6361 aaactcgtgc tgggaaattg ccacaacaag tcatttctaa aacagcaact catggttatt
     6421 cttcatatgg taaccagatt gggcttgcca caacctacgt tcgtgaatac ttccacccag
     6481 gctttgtagc taaacgtatg gaacttggtg ccgttgttgg tgcggctccc aagggcaatg
     6541 ttgtccgtga aaaacctgaa gcaggtgatg tgatcatcct tctcggaggt aaaacaggtc
     6601 gtgatggtgt cggtggtgcg acgggctctt ctaaggttca aacagttgag tctgtagaga
     6661 ctgctggtgc tgaggttcaa aaaggaaatg ccatcgaaga acgcaagatt cagcgcctct
     6721 tccgtaatgg caatgtcact cgtctgatca agaagtccaa tgactttggg gcaggcggtg
     6781 tctgtgtggc tatcggtgaa ttggcagacg gtcttgaaat cgacctcaac aaggtgcctc
     6841 ttaaatacca gggcttgaat ggtacagaaa ttgccatctc tgaatcacaa gaacgaatgg
     6901 cggtcgtggt tcgtcctgaa gatgtggata ccttcgttgc cgaatgtaac aaagaaaata
     6961 ttgatgctgt tgtggtggcg acagtaactg aaaaaccaaa tcttgtcatg cactggaatg
     7021 gtgagacaat cgttgacttg gagcgtcgtt tccttgacac caatggtgtg cgcgtggttg
     7081 tcgatgccaa agttgtggac aaggatgtca aactcccaga agagcgtcaa acatctgctg
     7141 aaacactgga atcagatacc cttacggttc tatctgacct caaccatgca agtcaaaaag
     7201 gattacagac tatctttgac tgctctgttg gacgctcaac ggttaatcac ccacttggtg
     7261 gtcgttacca actcacacca actgaggcat ctgtgcagaa attgccagtt caacacggtg
     7321 tgactcatac tgcgtcggtc attgctcaag gtttcaaccc atatgtagct gaatggtctc
     7381 cataccacgg tgctgcttat gcggttatcg aagcaactgc tcgtttggtg gctgctggtg
     7441 ccaactggtc caaggctcgt ttctcttacc aagagtattt cgagcgtatg gataaacaag
     7501 cagagcgttt cggtcagcca gtagctgctc ttctaggttc tattgaagca caaattcagc
     7561 ttggcttgcc atctatcggt ggtaaggact ccatgtctgg tacctttgaa gaattgaccg
     7621 ttccgccaac cttggttgcc tttggggtga cgacggcaga tagccgtaag gtgctctctc
     7681 cagaatttaa agctgttggg gaaaatatct actacatccc aggtcaagcc ctctctgcag
     7741 agattgattt tgacttgatt aagaaaaatt ttactcagtt tgaagccatc caagctgacc
     7801 ataaagtgac atctgcatca gctgtcaaat acggtggtgt agttgaaagt ttggctcttg
     7861 ctacctttgg aaactatatt ggtgcagagg tgaccttgcc tgaacttaaa acagctttga
     7921 cagctcaatt aggcggcttt gtcttcacat ctcctgaaga aattgctgga gtagagaagg
     7981 ttggacaaac gaaagcagac tttacactga ctgtcaacgg tgtgaagcta gatggacaca
     8041 agcttgacag tgcatttcaa gggacattgg aagaagttta cccaacagaa tttacccaag
     8101 cgaaagaact agaagaagta ccagctgtgg catcagatgt tgtgattaaa gccaaagaaa
     8161 aggttgaaaa acctgtggtt tacatcccag tctttccagg aaccaactca gaatatgatt
     8221 cagctaaggc cttcgaaaaa gaaggtgcag aggtcaattt ggtgccattc gtgaccttga
     8281 atgaagaagc tattgtcaag tcagttgaaa ctatggttga caatatcgac aagactaata
     8341 ttctcttctt tgctggtgga ttctcggctg cggatgaacc agatggttca gctaagttta
     8401 tcgtcaatat cctgcttaat gaaaaagtgc gtgtggctat tgatagcttt atcgcccgtg
     8461 gtggtttgat tctcggtatt tgtaatggat tccaagcctt agtcaaatcg ggtctcctac
     8521 cctacggaaa ctttgaagct gctaacagta ctagcccaac cctcttctac aatgatgcca
     8581 accaacacgt ggccaagatg gtggaaactc gcattgccaa taccaactca ccatggttgg
     8641 ctggtgtgca agtgggcgat atccacgcta ttcctgtttc gcacggtgaa gggaagtttg
     8701 tcgtgacggc tgaggaattt gcagagctcc gtgacaatgg acaaattttc agccaatacg
     8761 ttgactttaa cggtaaacca agtatggatt ctaagtacaa tccgaatggt tctgtccatg
     8821 ccatcgaagg aattaccagc aagaatggtc aaatcatcgg taagatgggc cattcagaac
     8881 gttatgagga cggtcttttc caaaatatcc caggcaataa agaccagcac ctgttcgcgt
     8941 cagcggttaa acatttcact ggaaaataag acttacagat tttctaatag atagtatcag
     9001 taatgtaaaa gtcatgtaaa tctagctctt gatgattaca aataaaaatt aggtataaaa
     9061 aatgacatac gaagtaaaat ctcttaatga agaatgtggt gttttcggta tttggggaca
     9121 tccagatgct gctaagttga cctattttgg actccacagt cttcaacacc gtggtcagga
     9181 gggggcagga atcctctcca atgatcaagg acaactgaag cgccatcgtg acatggggct
     9241 tttatcagaa gttttcagaa atccagctaa tttggataaa ttgacaggag ctggtgcgat
     9301 tgggcatgtg cgttatgcga ctgctggcga agcttctgtt gataacatcc aacccttcct
     9361 cttccgtttt cacgatatgc agtttggttt ggctcataat ggaaatctga ccaatgcagc
     9421 ctctctcaag aaagaactgg aacaaagagg agcaattttc agcgcgactt cggactctga
     9481 aatcttggct cacctcattc gtcgcagtca taatcctagc ctgatgggca aaatcaagga
     9541 agcgctcagc cttgtcaaag gtggttttgc ctatatcttg ctgtttgagg acaagttgat
     9601 tgcggctctt gaccctaatg gcttccgtcc gctttctatc gggaaaatgg ccaacggagc
     9661 agtagtcgtt tcctctgaaa cctgtgcttt tgaggtcatt ggtgctgaat ggattcgtga
     9721 tttgaagcca ggtgagattg tgatcattga tgacgagggc attcagtatg acagctatac
     9781 agatgatacc cagttggcga tttgttctat ggagtatatc tactttgccc gccctgattc
     9841 taatatccac ggtgtcaatg tccatacggc acgtaagaga atgggagcgc aattggcgcg
     9901 agaatttaag catgaggcag atattgtagt tggtgtgccc aattcttccc taagcgcggc
     9961 tatgggattt gcggaagaat caggcttacc aaatgaaatg ggtctgatca aaaaccaata
    10021 cacccagcga acttttatcc aaccgactca agaattgcgg gagcaaggag tgcggatgaa
    10081 actgtctgct gtttcgggtg ttgtcaaagg caaacgtgtg gtcatggtgg atgattccat
    10141 tgtacgtgga acaacctctt gtcgtatcgt tcagctcttg aaagaagcgg gtgcgactga
    10201 ggttcacgtt gccattggaa gtcctgcact agcgtatcca tgtttctacg ggattgatat
    10261 ccagacccgt caggagctga ttgcagccaa tcatacggtc gaagaaactc gccaaatcat
    10321 tggtgcggac agtctgactt atctttcaat tgatggcttg attgagtcga ttggtatcga
    10381 aacagatgcg ccgaacggtg gtctctgtgt cgcttacttt gacggtgact acccaacgcc
    10441 tctctatgac tacgaagaag actatcgtag aagtttggaa gaaaagacca gtttttacaa
    10501 gtaggcgaca gattctccat taaagaaaag gaaaaaacaa atgacaaata aaaatgcata
    10561 tgcccagtcg ggtgtggatg ttgaagcggg ttatgaagtt gttgaacgga ttaaaaagca
    10621 cgtggcccgt acggagcgtg caggtgtcat gggagctctt ggtggctttg gtggtatgtt
    10681 tgacctttcc aagactgggg ttaaagaacc cgtcttgatt tcagggactg acggtgtcgg
    10741 aaccaagctc atgttggcta tcaagtatga caagcacgat accatcgggc aggactgtgt
    10801 ggccatgtgt gtcaacgaca tcattgctgc aggtgcggaa cccctctatt ttctcgacta
    10861 cgtagcgaca gggaagaatg aaccagctaa gctagaacaa gtggttgctg gtgtggcaga
    10921 aggttgtgtg caggctggtg ctgccctcat cggtggggaa acggctgaaa tgccgggcat
    10981 gtacggcgaa gacgactatg acttggctgg ttttgcggtc ggtgtggctg aaaaatctca
    11041 aatcattgac ggttcaaagg tggtagaggg agatgttctt ctcggacttg cttcaagtgg
    11101 gattcactca aatggttact ctttggttcg tcgtgtcttt gcggattaca caggtgagga
    11161 agtcctacca gaattggaag gcaagaaact taaggaagtt ctacttgagc cgactcgtat
    11221 ctatgtcaag gctgtcttgc cgctcatcaa agaagagttg gtcaacggca ttgcccacat
    11281 cacaggtggt ggctttatcg aaaatgtccc tcgtatgttt gcagatgacc tagctgctga
    11341 aattgatgaa agcaaagttc cagtgcttcc aattttcaaa gcccttgaaa aatatggtca
    11401 gattaaacac gaagaaatgt ttgaaatctt caatatgggt gtgggactta tgttggcggt
    11461 cagccctgaa aatgtagagc gtgtaaaaga attgttggat gaagcagtct atgaaattgg
    11521 tcgcatcgtc aagaaagaaa acgaaagtgt cattatcaaa tgaaaaaaat agcggttttt
    11581 gcctctggta atggctcaaa ttttcaggtg attgccgaag aatttccagt ggagtttgtc
    11641 ttttcagacc atcgtgatgc ctatgtgctt gagcgtgcaa agcagctcgg cgttctgtcc
    11701 tatgcttttg aactcaagga gtttgagagc aaggcagact acgaagcagc ccttgtcgaa
    11761 ctcttggaag aacaccagat tgacttggtt tgcctagcag gctacatgaa aatcgttgga
    11821 ccaaccttat tgtcggctta tgaaggtcgg attgtcaaca ttcatccagc ctacttgcca
    11881 gaatttccag gagctcatgg gattgaggat gcttggaatg ctggcgtggg tcagtctggt
    11941 gtgaccattc actgggtgga ttcgggtgtg gatacaggcc aggtcatcaa acaggttcgt
    12001 gtgccacgac tagctgatga taccattgac agatttgaag ctcgcatcca tgaagcagag
    12061 tacaggttgt atccggaagt agtgaaggct ctatttacag attgactttt tgatgattca
    12121 tatgatatct ttgattttaa attggagtca gtgattgttg aagacggctt caaacggagg
    12181 tatttgtaat gttagaatct aaaaaaacaa ctcgatatgt attttatgtc tatctgatgt
    12241 tattaacttg gggaatctta tttaagtttg aaacaaatcc tgaatttata gcatttttct
    12301 tagctccaag gtatatcaat tggattccat tttcagaacc actaatagtc gatggaaaaa
    12361 ttgtttttgc tgaaatgtta tttaatctga tttcctttat tccattaggt gtttgtttcc
    12421 ctttgataaa aactaattta tctagtttaa gaatagtcgg gacaggtttc ttgattagtt
    12481 tattgtttga gtgcttacag tatattttag caataggtat aacagatata acggatttga
    12541 ctttaaatac gctaggtgtc tgtgtaggct tactgattta tcaaattttt ataagagtgt
    12601 tcaaatcaca gactagaaaa tggatcaata tcttaggtat gcttagcctt ggttttgctt
    12661 atcttgtttt actgttactg catttaattg gtgtttaact aatgattaaa aaggagaata
    12721 taatgactaa acgcgtctta atcagcgtct cagacaaagc gggcattgtt gaatttgccc
    12781 aggaactcaa aaaacttggt tgggagatta tctcaacagg tggaactaag gttgcccttg
    12841 ataatgctgg ggtggatacc attgctatcg atgatgtgac tggtttccca gaaatgatgg
    12901 acggtcgtgt gaagaccctc cacccaaata tccacggagg gcttctcgct cgtcgtgact
    12961 ttgatagcca cttggaagcg gctaaggaca acaagattga gctcattgac cttgtggtgg
    13021 tcaaccttta cccatttaag gaaactatcc ttaaaccaga tgtgacttat gctgatgcag
    13081 ttgaaaatat cgatattggt gggccgtcta tgcttcgttc agcagcgaaa aatcatgcca
    13141 gtgttacagt tgtggtagat cctgctgact acgctgtggt tttggacgaa ttggcagcaa
    13201 acggcgaaac ctcttatgaa actcgccaac gtttagcagc caaagtattt cgtcacacag
    13261 cggcttatga cgccttgatt gcagaatact tcacagctca agtgggtgaa agcaagcctg
    13321 aaaaactcac tttgacttat gacctcaagc aaccaatgcg ttacggtgag aatcctcaac
    13381 aagacgcgga cttttaccag aaagctttgc ctacagatta ctctattgct tcagccaaac
    13441 agctcaacgg gaaagaattg tcatttaata atatccgtga tgcagatgct gctatccgta
    13501 tcatccgtga cttcaaagat agtccaaccg ttgtggctct caaacacatg aatccatgtg
    13561 gaattggtca agctgatgac atcgagactg cttgggacta cgcttatgag tctgacccag
    13621 tatctatctt tggtgggatt gtcgtcctca accgtgaggt ggatgctgcg acagctgaga
    13681 agatgcacgg cgttttcctc gaaatcatca ttgcaccaag ctatacggat gaagcgctag
    13741 ctattttgat caataaaaag aaaaacttgc gtatccttgc cttgccattt aatgctcaag
    13801 aggctagcga agtggaagca gaatacacag gtgtagtcgg tggacttctc gtgcaaaatc
    13861 aagacgtggt caaggaaagc ccagctgact ggcaagtggt gaccaaacgt cagccaactg
    13921 agacagaagc gactgctctt gagttcgctt ggaaggctat caagtacgtc aaatcaaatg
    13981 gtattatcgt gaccaacgac cacatgacac ttggtgttgg tccaggtcaa accaaccgtg
    14041 tggcttctgt tcgccttgcc attgaccaag ccaaagatcg tctgaacggg gcggtccttg
    14101 cttcagatgc cttcttccca tttgcggata acgtggaaga aatcgccaaa gcaggaatta
    14161 aggccatcat ccagcccggt ggctctgtcc gtgaccaaga atccatcgaa gcagcggata
    14221 aatacggctt gactatggtc tttacaggtg tgagacattt tagacattaa gaagataaaa
    14281 gggaagaaaa cagtttcttt ccttttttgg cttaaaatgc taactgaaac aagattaaaa
    14341 cgaacttttt tgatataatg ttggtaaata attcgcaaaa gaggttgagg aatgaaactg
    14401 cttgttgtcg gttctggtgg tcgtgagcat gcgattgcta aaaagttact tgaatcaaaa
    14461 gacgtggaaa aagtctttgt agctcctggg aatgatggga tgactctgga tggtttggaa
    14521 ttggtaaata tctctatttc cgaacattct aaattgattg acttcgcaaa gaccaatgat
    14581 gttgcttgga cctttatcgg tccagatgat gcccttgctg ctggtatcgt ggatgatttt
    14641 aaccaagctg gacttaaggc ctttggtccg actagggctg cagcggagct ggagtggtcc
    14701 aaggatttcg ccaaggaaat catggtcaaa tacggcgttc cgacagcaac atatggcaca
    14761 ttttcagatt tcgaggaagc caaggcctat atcgaaaagc atggtgcgcc tatcgtagtc
    14821 aaggcggatg gcttggcact tgggaagggt gtcgtcgttg ctgagacggt tgagcaagcg
    14881 gtcgaagccg ctcatgagat gcttttggac aataaatttg gtgactcagg tgcgcgcgtg
    14941 gttattgagg aattccttga aggagaggaa ttttcactct ttgcctttgt caatggtgat
    15001 aagttctaca tcatgccaac ggctcaggac cacaaacgtg cctatgatgg cgacaaaggg
    15061 cctaacacgg gtggtatggg tgcctatgcg ccagtcccac acttaccaca gagtgtagtt
    15121 gatacagcgg ttgacaccat tgtcaagcca gttctagaag gggtgattaa agaaggtcgc
    15181 ccttatctgg gagttcttta cgcagggctt atcctgacag ctgatggacc gaaagtcatt
    15241 gagttcaacg ctcggttcgg agatccagaa actcagatta tcttgcctcg cttgacctct
    15301 gactttgctc aaaatatcac agatatcctg gatagcaagg agccaaatat catgtggacg
    15361 gacaagggtg tgactctggg tgtggttgtc gcatccaagg gctacccgct agactatgaa
    15421 aggggcgttg agttgccagc caagacagaa ggcgatgtca tcacctacta tgcaggggct
    15481 aagtttgcgg aaaatagcag agcactgctc tcaaacggcg gacgagttta tatgctcgtt
    15541 accacagcag ataccgtcaa agaagcccaa gccagcatat accaagaact ataccaacaa
    15601 aaaatagaag gactcttcta ccgaacagat atcggaagca aggcaattaa gtaaagatat
    15661 aagaataacg cgccgtagtc gccaaacacg ataatggtcg tcgtggtgaa aagaccagaa
    15721 cagtgaatgt tctggtcagg gggaaacttg gagaccgtag gctcaaagtt taggaatgaa
    15781 accgaaggtt tgcttacgcc tccatcacct aagaccatta tcaaaaagaa aaataaaaat
    15841 tcacaaaata cgttaatgat cgtatggttt gcgagcgtta gcgagctaat atagaacaat
    15901 caccgccgtt gtgaaagaac gattggatga taatccaatc gttcagggaa attggaagac
    15961 cttgggtttc caatttaggc atgagacacc tttggtggct gctgccgtcc ctcacaagct
    16021 aaggtgattg ttgaaaaaga ggaaaaagga gaagaaatga aaccagtaat ttccatcatc
    16081 atgggctcaa aatccgactg ggcaaccatg caaaaaacag cagaagtcct agaccgcttc
    16141 ggtgtagcat acgaaaagaa agttgtttcc gcacaccgta caccagacct catgttcaaa
    16201 catgcagaag aagcccgtag tcgtggcatc aagatcatca tcgcaggtgc tggtggcgca
    16261 gcgcatttgc caggcatggt agctgccaaa acaacccttc cagtcattgg tgtgccagtc
    16321 aagtctcgtg ctcttagtgg agtggattca ctctattcta tcgttcagat gccgggtggg
    16381 gtgcctgttg cgaccatggc tatcggtgaa gctggagcga ctaacgcagc tctctttgcc
    16441 ctccgtctcc tctctgtaga agataagtcc attgcggatg cacttgccaa ctttgctgaa
    16501 gaacaaggaa aaatcgcaga ggagtcgtca aatgagctca tctaaaacaa tcggaattat
    16561 cggtggcggt caactgggtc agatgatggc catttctgct atctacatgg gccacaaggt
    16621 tatcgcgctg gatcctgcgg cggattgccc ggcctctcgc gtggcggaaa tcattgtggc
    16681 accttataac gatgtggacg ccctccgtca gttggcagac cgttgcgatg tcctcactta
    16741 cgagtttgaa aatgtcgacg ctgacggttt ggatgccgtt atcaaggatg gacaactccc
    16801 tcaaggaaca gatctgctcc gcatttcgca aaatcgtatt tttgaaaagg actttttgtc
    16861 aaacaaggct caagtcactg tggcacccta caaggtcgtg acttctagcc tagacttggc
    16921 agatatcgac ttgtcgaaaa actatgtcct caagactgcg actggtggct acgatggtca
    16981 tggacaaaag gttattcgtt cagaagtaga cttggaagca gcctatgcgc tagcagactc
    17041 agcagactgc gtcttggaag aatttgtcaa ctttgacctt gagatttctg tcatcgtgtc
    17101 aggaaatggc aaggaggtga cgtttttccc agttcaggaa aatatccacc gcaacaatat
    17161 cctgtctaag accatcgtac cagcccgcat ttctgaaagt ctagtagaca aggctaaagc
    17221 tatggcagta cgaatcgcag aacaactcaa cttgtctgga actctctgtg tggaaatgtt
    17281 tgcgactgat gatgacgtta ttgtcaatga aatcgcccca cgaccacata actctgggca
    17341 ctattctatt gaagcctgtg atttctctca gtttgacacc catattctcg gtgttctcgg
    17401 agcaccatta ccagtcatca aactccatgc gccagccgtt atgctcaatg tcctaggtca
    17461 acacgtcgag gctgctgaaa agtatgtcgc agaaaatcca agcgcccacc tccacatgta
    17521 tggtaaaata gaagcaaagc ataatcgtaa gatgggacat gtgaccttgt ttagtgatgt
    17581 accggatagt gtggatgagc ttggggaagg gattgatttt taggacaagt ctatgataca
    17641 aattatcgtt aatacattta ttgaaaagga taagactgga gcagttgttg aagtgttgta
    17701 tgccagtgct gaccaagata aggtacaagc taaatatgaa gaactagctg cacaataccc
    17761 cgaaaattat ttagctatct atgatgtacc gctggatacg ggtttgaata cactagatca
    17821 ttacccgtct gtgtttattg gaaaagagga gtttgagtag aaatcttggt ttacctagat
    17881 agcttattcc caacagctta agaagaaagg aaaaattaac acatgatcaa ccgttactct
    17941 cgccctgaga tggcgaatat ttggagtgaa gaaaataaat accgtgcttg gcttgaggtg
    18001 gaaatcctct ctgacgaggc atgggctgag ttgggggaaa tccctaagga agatgtggct
    18061 ttgattcgca agaaggcgga ctttgacatc gaccgtattt tggaaattga gcaggagacg
    18121 cgccacgatg tggtggcttt cacgcgtgcg gtttctgaga ctcttggtga agagcgcaag
    18181 tgggttcact atgggttaac ttctactgac gtggtggata ctgcttatgg ttacctctac
    18241 aagcaggcca acgacatcat ccgtcgtgac cttgaaaact tcactaatat catcgctgac
    18301 aaggccaagg agcacaagtt caccatcatg atggggcgta ctcatggtgt gcacgctgag
    18361 ccgacaacct ttggtcttaa attagcaact tggtacagcg aaatgaaacg caatatcgag
    18421 cgcttcgagc atgcggctgc tggtgtagaa gctggtaaga tttctggtgc ggttgggaac
    18481 tttgccaata tcccaccatt tgtagaggag tatgtctgcg ataaacttgg catccgtgcc
    18541 caagaaatct ctacacaagt ccttcctcgt gaccttcacg ctgagtactt tgcggttctt
    18601 gccagcattg cgacttcaat cgaacgtatg gcgactgaga ttcgtggtct acaaaaatct
    18661 gagcaacgcg aagtagaaga gttctttgct aaagggcaaa aagggtcttc agcaatgcct
    18721 cacaaacgca acccaatcgg ttctgaaaat atgactggtc tggcgcgtgt cattcgtggt
    18781 cacatgatta cggcttatga aaacgtcgct ctctggcacg aacgcgatat ttcccactca
    18841 tcagctgagc gtatcatcac accagatacg accattttga ttgactacat gctcaaccgt
    18901 tttggaaata tcgtcaagaa cttgacagtc ttcccagaaa atatgatccg aaacatgaac
    18961 tcgacttttg gtcttatctt tagccaacgg gctatgttga cattgattga aaaaggcatg
    19021 acccgtgagc aagcctatga cttggtgcaa ccaaaaacag cctactcttg ggacaaccaa
    19081 gtagacttta aaccacttct tgaggcagat tcagaagtaa catcacgtct cacacaagaa
    19141 gaaatcgatg aaatcttcaa cccagtttat tacaccaaac gagtggatga tatctttgaa
    19201 cgtcttggac taggtgatta attaaaaaat aaacagcgag cttcaatctc gctgtttatt
    19261 ttttatcgaa aagacttagt cttcttttct tttagtgagt ccataggctg ctagtgtggc
    19321 catgagtcct gcgactacta gtcctgcaga atcgtgagtt cctgtttcag gaagtttttt
    19381 ctctattacc acaggagctg gatcttgagg aagagctttg ctttcctcag caggagcagt
    19441 tgatggagct ggttggcttg ggatttctag ttttggtttt tcttcagcaa tagcggcttg
    19501 tccgttttca tcgcctacat gtgttaccat agttccgact tcgactattt gagtaacggc
    19561 ttcctgtgct acgacactat ttacaagtgt tttcacttcc ttaccatcgg cagaagtgct
    19621 cacagagtag aagttgctac gatgtccatt gacgccctta gtaatgactt gtgtttttcc
    19681 tttgagtaag agtggatttt cacgagtcac tgtggtaaat ggaatttctt ctttttggat
    19741 atccagtcta ggttttacct cagtagttgg tgcaagacca ctttcatcac ccttgtgagt
    19801 tacaggagcg ccaacttcaa ccacttggtt tataacttct ttggttacct ggctatcaag
    19861 gactgtttct gttgtttttc cattttcagt gagtacagag atgtaatgag ttcgttcacc
    19921 tttgactcct gctgtgataa tattttcctg accagctggg aggttaggat tttctttctt
    19981 gataacttca aatggaattt cttcagttct tgtgatgagt tctggtctgg tttcaacatt
    20041 ggcagccact tcattttcat ctaggcttcc tgaatgagtt gcagctggtt tgaggccttg
    20101 aagagcggct tttaggttgg ctacaagtgt gtcaagctca gcttgtttat tacggttgag
    20161 gttgtaattt agagctgttt tagctgcgtc aagggcctca agactttctt tactatatcc
    20221 ttctaagttt gtaggaattt tagctaattc ttcgcggaga gcattataat tagcacggaa
    20281 gtagtctttg ttgtggtctg caaaggcagt catgagttca aagatttcct attccttgta
    20341 ttcagcgctt ggtctatctg cccagattga aagcatactt ccgactgttg gaagatctac
    20401 ttcaggatat ttggtagaag ctagttgatt gaatggaact ttaccagaat tttcaactgc
    20461 ttttgaaaga gggtaagctt catcttgttt gtgattgcct ataacataat accagtcacc
    20521 gttggtattc aagaacttat aacccttgct tgctaggtat tgaggtgatg cgaggttgta
    20581 tccccaccag cctttagacc agtaagaaat caagacatct ttgtcaaact gaacatcgtc
    20641 cttgtcttca tagtagaagc catcgttgaa ggccattggt tgaagccctc tttctttggc
    20701 catagctgcg agggtgttgg catattcggc aaatttgcca tagagttgat accacttgag
    20761 gtagtaccag ccttgggcac tagtcgcatc gttggcgtat tcgtcagtac caaagttgaa
    20821 aatctttgtt ttacctgcaa agaagtccat gtatttaccg atgagggctt ttacaaagtt
    20881 catcgcttct tcgtttttca agtccatagt tgtttttgaa actttatcaa agtgggcttg
    20941 aggattttta atacctaatt tttccatggc aaccagcata gcatccatgt gacctggact
    21001 gttaatagct gggatgagac cgatgtcctt agatttagcg tattcaatta gctctgtcac
    21061 ttctgcctgt gttagtgcag taccgtttgg atcgtcgtag taagctttag ttccttcgat
    21121 aatagctttt ttaacgtcat cactagcata ggtttttccg ttggcagtaa tggtcatatc
    21181 atcgagtaga aagcgaagtc cgtcatttcc tagaaggaga tggacatcag aatatccgag
    21241 ctcactggcc ttgtctacga tgcgtttgag ctggtccaga gtaaagtatt tgcgtccagc
    21301 atcgattgag attaccttgt ttttggcaag tttttcaacc tcacgtttag cttcttcttc
    21361 tttttgagct tcaggcgtga gggtcaagtt gttgacagtt tcttgaagtt tagcaatggc
    21421 ttgatcaatc gtatcttgtt gggcacggct aaggttgcta tcgagagagc gaatagcttt
    21481 ttcagcttct tttacggccg tgacgctttc tgcagtataa cggttcaggt cttttggtac
    21541 ctcgttaagt gcttgctctg cagattcata atcagctgcg aagtattcag cgttggcatt
    21601 tgcaaaatga cgcatgagtt tgaagaggcg tgatggtgaa taacgtgcag atggagtgtc
    21661 agcccaagca gctaccatac caccgatgat tgggatatca gctccttctg tttttggtac
    21721 agaagtgatt ggtgtgtttt taataccatt gagcccctga tcgagattgt accagccttg
    21781 gccatcagcg tttcgtccaa gaacgtagta ccaagcatca ttggtattaa ggatttggtg
    21841 acctttttca gctagtagtt tagaagaagc gacatcgtag cctccccaac caccagtcca
    21901 catagaaacg atgatgtctt tgtcaaaact accaaagctt gtgtcgctat tgtagtagat
    21961 accgtcgtta aaggccattg gtttgagacc gtgcgatttt acaatacgag cgaggtcatt
    22021 ggcgtaggca ataaattttt catagccttt tacagggtag ccttcgtttg gatagtattt
    22081 atcagcttga agcacactcc aacctttagc atctgtcgca tcattggcat attcatcaag
    22141 tccgatgttg aagatttcag tctttttcgc gaaataagca gcatacttgt cgataagggc
    22201 ttttgtaaaa gcgacagctt gttcgttgtc aagatcgaca gtacgggctg atttcttccc
    22261 aaaatagcta aagttagggt tttggattcc caattctttc atggcattga gaatcgcatc
    22321 catgtgtcca ggactattta ctgtcggaat gagaccgata cctttatctt tggcatagtt
    22381 aatcagatct gtcatttgac tttctgttaa gtgattgccg tttggatcgt tgtaataatc
    22441 atttgtacct ttttcaatgg cgcgtttgac atcgtcactg gcataggtct tgccgttagc
    22501 tgtgatgctc atatcgtcca acatgaaacg gagtccatca tttccgacta ataggtgtaa
    22561 atcagtgtag ccataatgtt tcgctttatc gatgatttcc ttgagctgtt ctggtgagaa
    22621 atatttacgt ccagcatcaa tagaaacaat tttctttttc gctagttttt catttacagt
    22681 tgcagcacgt tcctttcctg cctctgttgc cggtttgtca gcctctgctt tcgcttcatc
    22741 ttttttagct ggtttatcct tgtcagtctt gtctgtattt gactctttag aatcaacctc
    22801 tttcgcttct tcctttttag ggctagcttc ttctgccttt ttattagcag tttctttttc
    22861 agcagaagtt ggagttacca cttctgcttt atcactagga gttgaactaa cttcctcttg
    22921 tggtttttct tctgtttttg gaagactagc taccttatca gtagctggag tttctgtttc
    22981 tacagttttt ggagcttctg gttgaagcac tgctttaggt gtttcctcag tccgattttc
    23041 ggatgattga ggggaatcag aaaccgtatg gatggtcggt tggttttctg tagtagtagg
    23101 agtaacttca tcggctgcaa cagtctgtgc ttggaaggca aatccaatta gaacagaagc
    23161 tgctcctaca gcgtatttac gaatagaaaa acgctgttgt ttttcatgtt tcattgcaaa
    23221 acctcctgat tgcattgtta tattgatagc gattatataa atcaacgcct ttattttatt
    23281 tcttatatta acgagagtca agaggagatg acaaa
//
LOCUS       DAVKJD010000022        22783 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKJD010000022 DAVKJD010000000
VERSION     DAVKJD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22783
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.022"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..421)
                     /locus_tag="VW157_000943"
     CDS             complement(<1..421)
                     /locus_tag="VW157_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9552265.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYNDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLVALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEY"
     gene            600..1367
                     /locus_tag="VW157_000944"
     CDS             600..1367
                     /locus_tag="VW157_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEU9552266.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            1452..1727
                     /locus_tag="VW157_000945"
     CDS             1452..1727
                     /locus_tag="VW157_000945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552267.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFLQQYQQI"
     gene            1817..2131
                     /locus_tag="VW157_000946"
     CDS             1817..2131
                     /locus_tag="VW157_000946"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEU9552268.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(2232..4784)
                     /locus_tag="VW157_000947"
     CDS             complement(2232..4784)
                     /locus_tag="VW157_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9552269.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTIPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFIFVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDILAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4846..6468)
                     /locus_tag="VW157_000948"
     CDS             complement(4846..6468)
                     /locus_tag="VW157_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9552270.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            6674..7699
                     /gene="trpS"
                     /locus_tag="VW157_000949"
     CDS             6674..7699
                     /gene="trpS"
                     /locus_tag="VW157_000949"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9552271.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            7851..9329
                     /gene="guaB"
                     /locus_tag="VW157_000950"
     CDS             7851..9329
                     /gene="guaB"
                     /locus_tag="VW157_000950"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9552272.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(9380..10477)
                     /gene="recF"
                     /locus_tag="VW157_000951"
     CDS             complement(9380..10477)
                     /gene="recF"
                     /locus_tag="VW157_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9552273.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(10480..10848)
                     /locus_tag="VW157_000952"
     CDS             complement(10480..10848)
                     /locus_tag="VW157_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9552274.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SCRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            11007..12257
                     /locus_tag="VW157_000953"
     CDS             11007..12257
                     /locus_tag="VW157_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9552275.1"
                     /translation="MELVHGISIHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKIMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            12254..13537
                     /locus_tag="VW157_000954"
     CDS             12254..13537
                     /locus_tag="VW157_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9552276.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            13579..14400
                     /locus_tag="VW157_000955"
     CDS             13579..14400
                     /locus_tag="VW157_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9552277.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            14411..14956
                     /gene="pgsA"
                     /locus_tag="VW157_000956"
     CDS             14411..14956
                     /gene="pgsA"
                     /locus_tag="VW157_000956"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9552278.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            14953..15780
                     /locus_tag="VW157_000957"
     CDS             14953..15780
                     /locus_tag="VW157_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9552279.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRKIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYNLPENYLTESELEDKLWELL"
     gene            15765..16604
                     /locus_tag="VW157_000958"
     CDS             15765..16604
                     /locus_tag="VW157_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9552280.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            16597..17391
                     /locus_tag="VW157_000959"
     CDS             16597..17391
                     /locus_tag="VW157_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9552281.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            17450..18268
                     /gene="mreC"
                     /locus_tag="VW157_000960"
     CDS             17450..18268
                     /gene="mreC"
                     /locus_tag="VW157_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9552282.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGAFENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            18268..18762
                     /gene="mreD"
                     /locus_tag="VW157_000961"
     CDS             18268..18762
                     /gene="mreD"
                     /locus_tag="VW157_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9552283.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            18856..20034
                     /locus_tag="VW157_000962"
     CDS             18856..20034
                     /locus_tag="VW157_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9552284.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVYESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            20258..21037
                     /gene="rpsB"
                     /locus_tag="VW157_000963"
     CDS             20258..21037
                     /gene="rpsB"
                     /locus_tag="VW157_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9552285.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNSDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            21115..22155
                     /locus_tag="VW157_000964"
     CDS             21115..22155
                     /locus_tag="VW157_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9552286.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            22401..22724
                     /gene="tnpB"
                     /locus_tag="VW157_000965"
     CDS             22401..22724
                     /gene="tnpB"
                     /locus_tag="VW157_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9552287.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGERKDRFKALYWDGQGFWLLYKRFENGKLTWLSTEKDVKALAPEQVDWLMKGFS
                     ITPKI"
BASE COUNT         7286 a         3632 c         4650 g         7215 t
ORIGIN      
        1 tatattctaa ttcggtggta tttagattca gttgcataaa tcgcttttgt aaatctgtac
       61 taagcatgat atgaagttta tttcgtaaga aattttggat ttctttagtc ccattttcag
      121 aaagaagggc aaccagaagt ggttctgact ctagatattc aaaaacttct aaaatagcgt
      181 ctcttttgtg atgagcatgt ttttgaaaaa tatattcaaa tgtatggaat agcttgcttt
      241 gatagtgctc aatcatatca tacttatcat tatagtgagt atagaagctg gaacgactaa
      301 ttccggcttt ttctactaat ttgacagtag aaattttatc aaatggctgt tccatcagta
      361 attgtaccat agcattttca atagttcgct ttgtttttaa gcgtttgtta ctttcttgca
      421 tatttcctcc ttgtaaacaa attagactat atgtctaaaa atagattttt tatcttgtaa
      481 tttagatttt ttaatgtata atctattata tcaaaatttt agacaatatg tttaaaaaag
      541 gagaaactaa gtttaaagaa tggaaagcaa tttaaaaaaa accaaccttt attattgtca
      601 tgatcgggat ttctcttatt ccagatctgt acaatatcat atttttgtca tcaatgtggg
      661 atccatatgg gcaattgtct gacttacctg tggcagttgt aaataatgat aaagaggctt
      721 cctataatgg taatactatg gcaataggaa aagacatggt gtccaattta aaagaaaata
      781 aaaccttgga ttttcatttt gtagatgaag aggaaggaaa gaagggattg gaagatggcg
      841 attactatat ggtagtgact ttaccaagtg atttatctga aaaaacaact acattatcca
      901 atattcaatc gacagcagct tatcaatcat tgacaagtga gcaacaaact gagataagtg
      961 attctgtatc tcaaaattca actgatagta ttcaatcggc tcagtcaatt gtagctttag
     1021 tacaagattt acagggaagt ttagaaaact tacaaaatca atcttctaat ctttcgactt
     1081 taaaaaatca atctaatcaa gtatcaccta ttacttctac ttctttgata ggattgtcaa
     1141 gtggattaac agagatacaa ggagatgtta ctagcaaatt agttcctgcc agtcagtcga
     1201 ttgcatcagg tgtaaacgca tatactacag gtgttgataa agtttctcag ggcgcaagtc
     1261 aactaagtga aaaaaatgcc accttgacag gtagtttgga taaactagtt tcaggctcaa
     1321 acaccttgac acaaaaatct tctagattga cagcaggagt tggttaatta caatcaggat
     1381 ctgggcaatt agcagacaaa tccagtcagt tactttcagg tgcttctcca ttagagaata
     1441 gagctaataa attggcagat ggatctggga aactagcaga aggtggaaca aagttaactt
     1501 ctggattgga agatttacag acaggacttg cttctttagg acaaggacta ggtaatgcta
     1561 gtgatcaact caaatcagta tcaacagaat ctaaaaatgc agagattttg tcaaatccac
     1621 tcaatctttc aaaaacagac aatgatcaag ttcctgtaaa tggaatcgca atagctcctt
     1681 atatgatatc agttgctctt tttttgcagc aatatcaaca aatatgatat ttgcgaaatt
     1741 gccttcagga cgtcatccag agagccgttg ggcttggttg aaatcttgag ctgaaataaa
     1801 tggtattata gctgttttgg caggaatttt ggtatatgga ggagttcagc ttattggttt
     1861 aactgctaat catgagatga gaatatttat tctcatcatc ctaacaagtt tagtattcat
     1921 gtctatggtg accactttag caacgtggaa tagccgtata ggagcttttt tctcacttat
     1981 tttgctttta ctacagttag catcaagtgc aggtacttat ccacttgctt tgacaaatga
     2041 tttctttaga tctattaatc cctggttacc aatgagctat tcagtttcgg gattacgaca
     2101 aacaatctct atcaacaagt cattttccta gctgtcatac tagttctatt tactagttta
     2161 ggtatgctag cctatcaaca taagaaaatg gaagaagatt aaaaaaatcg accgattaac
     2221 tggtcgattt tttatgcctt agatgacttt cgtctgtgat tatagattcc aaatagtaag
     2281 agagaagtaa aggaacagat tgctccagta ataaaaccat tgggaatgaa ggaaagtgta
     2341 atagttcctt tccccttggg aatgtcaact ttcataaatc cagtttgagc ttgtttaatt
     2401 tctattttct taccatcttg gtaggcagac caacctttgt cataaggaat ggtgaagaaa
     2461 atagatgtat cttgttggac atcatatgta gcaaaaacct tgtttttaga agttgatact
     2521 gtgacaggtt gttctttaat tttttgaatt gcctcggtga aagttttggt atctaaacga
     2581 tagaaggtag gagattcaaa tgatacttgt gaatttccag ggaaactaac attgatattg
     2641 aaagtttttt tctctttagt atatcctaga ttaaagaagg agaagacatt atcagttgta
     2701 aaagtctttt tttcaccatt tacaaggatg tcaaccttct tttgtttatc gttagaaaag
     2761 tgaaggttta tgaaagagag ataaacttgg ctgttttctg gaacttcaat ttgatactgg
     2821 attgctgcat cttcatttga agaacttgtg acactaatca aatcattagt attttctatt
     2881 ttttctgttt tttcataagg tattggagaa aaataatcaa aattgacgtt agcaagttga
     2941 tttaaaaatg aggcctgatt atccaaggta tgttcattga acttgacatc attgtaaaca
     3001 gattgactag caactgcaat cggaagagag tattgatttt catatagggt aagattatct
     3061 ttttgataga tatctttaaa gccatactta tcaataggac tgtctgagat attgtactgg
     3121 ataccaaata aactatcagc caaaatacta ttatttgcat atcggagatt gagattagtc
     3181 ccagaggatt taaaaccaag tttatctaaa gtagagcttg atgaacgatt tcgaacagat
     3241 gaaaattgag agattccatt gtagttgaat ttcatactgt catttcctgt ctgagtttgt
     3301 agtttttcag tacgagtaaa ttgatttcca atatatgttg agaaagattc catagctagg
     3361 atatctcgac tataagcact tcgagaagca aatccccatt ccttagcaat tccgtccatt
     3421 tgagatgaag catttaaact catttcaacc agtataaata aagagattag aatggcaaat
     3481 agattcacaa atataaactt tttgataact gcaaggagta aaagagaata gacaaccaaa
     3541 aattcaagag taagcagaat attcaaatct gttaaaaaag aataatgcga ttttagatag
     3601 atggtagcta aaaatcctgc tactacaaga aaaagcgaaa ctaaaaaatt ccagacttta
     3661 agttctttca gacgctttaa gacttctgct gctgtgtaaa ttaacaaggt agagaaaatc
     3721 caagcatagc gatgtaaaaa catgtttgga gtatgcatgc cttgccaaaa taagtcaaga
     3781 gcttctatgt aaaagcttgc aattagaaat gcaaagaata ttacatatat gagtttcacg
     3841 tgaaacttaa tagatttcag cgtaaaaaat aaaatggtca aaataaaggg aaatagtcca
     3901 acaaaaatca ttgggatggc cccatacttt gttgtgtcaa aggaaccaat gaattgctta
     3961 gcaaagagat caagatacca gctactttca gtttgaaact ttgtaacttc agtcaatttt
     4021 tccccatgtg tctgtaaatc aaatagagtg ggaatagtca taatcaaact agccatacca
     4081 gctaaaaagg agataactat gaaatcaaga acagatgatt ttcgagtctt aaagtcccac
     4141 gaaatttgac agagatacca gaaaataaga aacaatactg tcatatatcc aaaataataa
     4201 ttttgaataa ataagattga cagacttgta aagtacaata ggagtttctt ttcagttatc
     4261 agtagatgta aaccagttat aattaaagga atcaagataa aaacatctag ccaggttttt
     4321 atctctaatt gactgacagt gaaactcatc agagcatagg aagtagataa ggctagtttt
     4381 aaagtctgag ggatagattg aaacaattta ttcaaactaa aaaaggttga cagaccaatc
     4441 aatccaaatt ttaagagagt ggtcagatag acagcatctg gcatattcgt tagatcaaaa
     4501 aagtaaacta gaggcgcgag aaaactaccc aagtaataac tagatagggc atagaagttt
     4561 agccctagac cacttgtaaa ggtgtaaaac agactactat ttccatgtag gatatttcgt
     4621 aaggctacat caaaaataac gtattgatga aagccatctc ctaatagagg agagttgtcg
     4681 ctattccagt agataccttg agatagatat actccagaca taatcactac aggaatgatg
     4741 aaagaaataa aataggttcg atatgttttt aaaaatgatt tcatgttacc tcgtagaatg
     4801 atagaaaact cagttggtta acccaactga gttttgaagt tttatttagt ctttccaaag
     4861 ttctttaact tttgcttgta cttctgcatt ttctaggaat tcatcgtagg tttcatcgat
     4921 acggtcaatg acgccatttt tagataagac aatgatatgg ttagccaaag tttgaataaa
     4981 ttcgtggtca tggctagcaa agatgattga ttctttaaag tttttcaatc catcattcaa
     5041 gcttgagata gattccaagt ccaagtgatt tgttggatca tcaagtacaa ggacatttga
     5101 ttttaagagc atgagttttg aaagcatgac acgaactttt tctccccctg acaagacatt
     5161 tacaggtttg ttaacttcat ctccagagaa gagcatacgg ccgaggaaac cacgtaggaa
     5221 agtattgtca tcttcttctt tacttgcgaa ttgacgcaac cagtcaagaa ttgattctcc
     5281 tcctgcaaaa tcagctgagt tatcttttgg taggtaagat tgactagttg taactcccca
     5341 cttgacagtt ccttcatagt caatatctcc catgattgca cgaattaatg cagtcgtttg
     5401 aatatcattt tgtccaataa gtgctgtctt atcatctgga cgcaagatga aactaatatt
     5461 atccaagata gtttcaccat caatctttac agttagattt tctactgtca agagatcatt
     5521 accaatctca cgttccgctt taaagttgat aaatggatat ttacgactag atggtacaat
     5581 ctcttctagc tcaatcttat caagcatttt cttacgtgat gttgcctgcc ttgacttaga
     5641 agcattggca gagaaacgag caacgaattc ttgcaattgt ttaatttttt cttctgcttt
     5701 agcattacgg tctgctagca atttagcagc aagctcagaa gattccttcc agaagtcgta
     5761 gtttccgaca tagagtttga tttttccaaa gtcaaggtcg gccatgtgag tacaaacttt
     5821 gtttaagaag tgacggtcgt gggatactac gataactgtg ttatcaaagt caatcaagaa
     5881 gtcttctaac caagtaatcg attggatatc caaaccgtta gtaggctcgt ccaagagaag
     5941 aacatctggt ttaccaaaaa gtgctttggc gaggagaacc tttacttttt caccgttggc
     6001 caattcgctc atgttttggt agtgtaattc ttctggaatg tttaggtttt gaagtagttg
     6061 agaggcttca ctctctgctt cccaacctcc aagttcggca aactctcctt cgagttcggc
     6121 agcacgaacc ccgtcctcgt ctgagaaatc ttccttcatg tagatagcat ctttctcttt
     6181 catgatgcta taaagttttt catttcccat gataacgaca tcaatggcac gttcatcttc
     6241 gtagtcaaag tgattttgac gaagaacaga gagacgttca tctggaccaa gagagatgtg
     6301 accagtagta ggttcgatat ctccagctaa aatttttaaa aaggttgatt ttccggcacc
     6361 attagcaccg attaatccgt aagtatttcc ttctgtaaat ttgatattga catcatcaaa
     6421 aagtttgcga tcactaaaac gtagtgaaac atcagatact gtaagcaatg tttttctcct
     6481 atatgtgtaa tatatttatt ctactagaaa atacagaaat attcaaattt ttatttgtca
     6541 attttatgta aattatattt acagtatcct ttacacaaat ctgtaaaaag caaggctgat
     6601 ttattttgat aaattacggt tatttcatta aaaaaatgct ataattgaaa ggactatatc
     6661 gaaggagaac aaaatgacta aacccattat tttaacagga gaccgtccaa caggaaaatt
     6721 gcatattgga cattatgttg gaagtctcaa aaatcgagta ttattacagg aagaggataa
     6781 gtatgatatg tttgtgttct tggctgacca acaagccttg acagatcatg ccaaagatcc
     6841 tcaaaccatt gtagagtcta tcggaaatgt ggctttggat tatcttgcag ttggattgga
     6901 tccaaataag tcaactattt ttattcaaag ccagattcca gagttggctg agttgtctat
     6961 gtattatatg aatctagttt cgttagcacg tttggagcga aatccaacag tcaagacaga
     7021 gatttctcag aaaggatttg gagaaagcat tccgacagga ttcttggtct atccaatcgc
     7081 tcaagcagct gatatcacag ctttcaaggc taattatgtt cctgttggga cagatcagaa
     7141 accaatgatt gagcaaactc gtgaaattgt tcgttctttt aacaatgcat ataactgtga
     7201 tgtcttggta gagccggaag gtatttatcc agaaaatgag agagcagggc gtttgcctgg
     7261 tttagatgga aatgctaaaa tgtctaaatc actaaataat ggtatttatt tagctgatga
     7321 tgcggatact ttgcgtaaaa aagtaatgag tatgtataca gatccagatc atatccgcgt
     7381 tgaggatcca ggtaagattg agggaaatat ggttttccat tatctagatg tttttggtcg
     7441 tccagaagat gctcaagaaa ttgctgatat gaaagaacgt tatcaacgag gtggtcttgg
     7501 tgatgtgaag accaagcgtt atctacttga aatattagaa cgtgaactgg gtcctattcg
     7561 tgagcgccgt attgaatttg ctaaggatat gggagaagtt tataatatga ttcaaaaagg
     7621 tagtgaaaga gcgcgtgaag ttgcgggtca aaccctatct gaggtaaaag gggcaatggg
     7681 acttcattac tttaactaag tttattttac aagaaactat cttttctatc tcgaagaaaa
     7741 gatagttttt tatttacccc tgtaacattt gacaaatttt tatagaatgg tatgatagat
     7801 acaatataaa aagagtcaag ctcaaaaaga aagaaaagag gaaacttcga atgtctaatt
     7861 gggacactaa atttttgaaa aaaggtttta cctttgatga tgtattgctt attccagctg
     7921 aaagtcatgt gttgcctaac gatgcagatt taacaactaa attggcagat aatttgactt
     7981 taaatatccc aattattacc gctgccatgg acacagttac agagagtcaa atggccattg
     8041 ctattgctcg tgcaggcggt ctcggagtta tccataaaaa catgtcaatt gctcaacaag
     8101 cagacgaggt tcgtaaggta aaacgttctg aaaatggagt tattattgat ccgttcttct
     8161 tgacgcctga acatacaatt gctgaagcag atgagcttat gggtcgttac cgcatcagtg
     8221 gtgttccagt tgttgaaaca cttgaaaatc gtaaattggt tggtattttg acaaaccgag
     8281 atcttcgttt tatttcagat tataatcaac caatttcaaa ccatatgact agtgaaaatc
     8341 ttgttactgc tcctgtgggt acggatcttg caacggctga gagtattctt caagagcatc
     8401 gtattgaaaa acttccgttg gtcgatgaag aaggcagtct ttctggtttg atcactatca
     8461 aagatattga aaaagttatt gagtttccaa atgcggctaa agatgagttt ggtcgtcttc
     8521 tagttgcagg tgcagtaggt gttacttcag atacatttga acgtgcagag gctctttttg
     8581 aggcaggagc ggatgcgatt gttattgata ctgcacatgg tcattctgca ggtgtcttgc
     8641 gtaaaattgc cgagattcgt gctcatttcc cagatcggac tttgattgct ggaaatattg
     8701 ctactgctga aggtgcacgt gccctttatg aagcgggtgt agacgttgtt aaggttggta
     8761 ttggaccagg ttctatctgt actactcgtg tgattgctgg tgttggtgtt ccgcaagtaa
     8821 cagctatcta cgatgctgca gctgttgcgc gcgaatatgg taaaacgatt attgctgacg
     8881 gtgggatcaa gtattctgga gatattgtaa aagcacttgc tgcaggtgga aatgctgtta
     8941 tgcttggatc tatgtttgct ggaactgatg aagctccagg cgaaactgaa atcttccaag
     9001 gacgtaaatt caagacttac cgtggtatgg gatcaattgc tgctatgaag aaaggttcaa
     9061 gcgaccgtta tttccaaggt tctgtcaatg aagcaaacaa gcttgttcca gaaggaattg
     9121 aaggtcgtgt tgcttataaa ggagcggcag ctgatattgt tttccaaatg attggtggta
     9181 ttcgctctgg tatgggttac tgtggtgcag ctaaccttaa agaactacac gataatgctc
     9241 aatttattga aatgtctggt gctggtttga aagaaagcca tcctcatgat gtgcaaatta
     9301 ctaatgaggc accaaattat tctatgtaaa aaacaatgaa aagaactcca gtgaaaacag
     9361 gagttctttt acaatgttgt caatttccat ttacagcagc tttaccatcc tgaatagtga
     9421 agatacttag attttctggc agattttgaa gatggtctaa gcttgttgtt gtgataaagg
     9481 tttggattga ttgagaaatc gtttctaata attttaactg tctagtgttg tcaagttcac
     9541 tcatcacatc gtcaagcagt aatataggag attctgtggt aatgctttcc attaattcga
     9601 tttctgctaa ttttatcgag aggacgagac tacgatgttg accttggctt ccgaaactag
     9661 catccatccc atttatataa aaagaaatgt catctcgatg aggaccgaca ccagtattct
     9721 ttttaaataa atctctggat ctactttttt ctaaagcaat tttgaaagat tcggataagt
     9781 tttgtttgtc agttatattg acagaagatt gataggatat tgacaactct tcgatctgat
     9841 tagagagttc aaaatgtttc ttacgcccaa atgattctag tttttttatg aaatctaagc
     9901 ggtgattcat tacacgacat ccataatcaa ctagctgatc atctaacaca gaaaggaatg
     9961 tttcatctat tttttgagct gattttaggt aagtgtttct ttgctttagg atgtggttat
    10021 aattggttaa gtcagataaa tagattggct taatttgccc aagttccata tcaatgaatt
    10081 ttcgtcgaat cgaaggtgct cctttaatta gttgtaaatc ttcaggagca aataagacaa
    10141 cattcatgtg tcctacataa tctgaaaggc gtgcctgttt taagtgatta acttttgtta
    10201 cacggccttt ttgtgttagt tcgatttcta ggggaatgga tccagttttt ttctgaacga
    10261 gacctgaaag atgaagttgt tcctcatcaa aatgaatgag atttttatct gttcgagttc
    10321 gatgactacg cgttaaggct aaaaaataga tagcctctaa catgtttgtt tttccttgtg
    10381 cattacgtcc caaaaagaca ttcaatttag gattaaagtc tattttcgtt tctttgtaat
    10441 tacgaaaagt cttgagagat agatgttgta gccacatgat tatctaccag ggaatcgtgg
    10501 agcttgttgg cttttaggtg atgaagcagg tttcgatttg tcttttttaa ctcccttatt
    10561 catctttttg acaagtttag cgatccgttc tttttcaact ttatcagctt ggtattcatc
    10621 ttgttcttca gaagtaggtt gtgtcaacaa gatgtcaatg ttcatgtcag ggatgtcaac
    10681 tttatcgcca atacgaagtt tttttccacg acaactctct aattccctat taaagtaaac
    10741 agaatgttca gagagaaatg atttaatagc tcctccgcta tgtgtaattc caagttcttt
    10801 gagtagtgct tggagggtaa taaattcttc aaataatttg tattccataa ttcacctcaa
    10861 tctttggtat tataccatat tttcctaaaa atagtgagag agacagctag aaatgtaagc
    10921 gatttttatt tcaaaagtgt tacagggaac aagaaaattt caggttttcg tgatataata
    10981 gaagtctgta tataaggagg taaatcatgg agttagtgca tggaatttca atacatttta
    11041 tccaatcaaa aaagtttaaa acaaacaaaa ttaccgtgcg ttttaccgct ccattatccc
    11101 ttgatacgat tgcaggtcac atgttgagtg caagtatgct agagactgct aatcagatgt
    11161 accccacttc tcaagatttg aggagacact tggccagtct atacggtaca gatatgtcaa
    11221 ccaattgttt cagaagaggg caaagccaca ttatagaatt gacatttacc tatgttcgtg
    11281 atgagttttt aagtaggaaa aacgtgctaa cctctcagat tttggaactt gtaaaagaaa
    11341 ctcttttttc acccgcagta gttgataatg ggtttgatcc ggccttattt gaaattgaga
    11401 aaaaacaatt gctagcaagt ttagcagctg atatggatga ttctttttat tttgcacata
    11461 aagaattgga taaattgttt tttcatgatg aacgtcttca attggaatat agtgatttac
    11521 gaaatcgtat tttagctgaa actccacaaa gttcttattc ttgtttccaa gaatttttag
    11581 ccaatgatcg aatagatttc tttttcctag gtgattttaa tgaggttgaa attcaaaatg
    11641 tattagaatc atttggcttt aaaggtcgaa aaggagatgt gaaggttcag tattgtcaac
    11701 cttattctaa tatccttcag gaaggtatgg ttcggaaaaa tgtgggacaa tccattttgg
    11761 aattaggtta tcattaccgt tctaaatatg gtgatgaaca acatttaccc atgattgtaa
    11821 tgaatggttt acttggtgga tttgctcact ctaagctctt tacaaatgtc cgtgaaaatg
    11881 ctggattagc ttataccatt tcaagtgagc ttgatttatt tagtggattc ttgaggatgt
    11941 atgctggtat caatcgagaa aatcgtaacc aggctcgtaa aataatgaat aatcaactgc
    12001 ttgatttaaa aaaaggttat tttacagagt ttgagttaaa tcagaccaag gaaatgattc
    12061 gttggtcgtt gttactttct caagataatc aatcttcatt gattgaacgt gcttatcaaa
    12121 atgccttatt tggaaaatct tcagcagact ttaaaagttg gattgcaaag cttgaacaaa
    12181 ttgacaaaga tgctatttgt agagtagcta ataatgtgaa actacaagcg atttacttta
    12241 tggaaggaat agaatgacaa aggttgtttt tgaagaaaaa tactatccag ctgtaaaaga
    12301 aaaggtttat cgaactcgtt tggccaacgg attgacagtt gctcttttgc ctaaaaagga
    12361 atttaaagag gtttacggga gtgtcactgt acagtttggt tcggtagata cgtttgtcac
    12421 agaagttgac ggagatgtaa aacaatatcc tggaggaatt gctcattttc ttgaacataa
    12481 attatttgag agagaagatt ctagtgattt gatgtcggct tttacgagtc taggtgcaga
    12541 tagtaatgcc tttacaagct ttacaaaaac aaactatctt ttttcagcaa cggattattt
    12601 tttagaaaat ttagatttac ttgatgaatt ggtaacatca gcacacttta ctgaagcttc
    12661 cattctgaca gagcaggata ttattcagca agaacgagaa atgtaccaag atgatccaga
    12721 ttcgtgttta ttcttttcaa ctttagcgaa tttgtatcct ggtacacctt tagcaactga
    12781 tatagttgga agtgaggagt ccatttccca aatcaatcta actaatttgc aagaaaattt
    12841 tacaaagttt tacaaacctg taaacatgtc tctgttttta gttggtaatt ttgatgtgga
    12901 gcgagtacag gactattttg aaagcaaaga actgaaagat tcagattttc aggaagtagc
    12961 aagagaaaag ttgtttttac agcctgtaaa gccaacagat agtatgagaa tggaagtatc
    13021 ttctcccaaa ctagcgattg gagttagagg taagcgagaa gtttctgaag cggattgcta
    13081 tcgacatcat attttattaa aattattgtt tgcaatgatg tttggttgga cttcggatcg
    13141 ttttcaaaaa tgttatgaat caggtaaaat tgatgcgtcc ttatctctgg aagttgaaat
    13201 aacaagtcgc tttcattttg tcatgttgac aatggatacg aaagagccag ttgctttgtc
    13261 tcatcaattt aggaaggcta ttcgtaattt tacaaaggat ttagatatta cagaggaaca
    13321 tttagatatt atcaaaagag agatgtttgg cgaatttttc agtagcatga actctcttga
    13381 atttattgca acgcaatatg atgcttttga aaatggtgag acaatttttg atttgccgaa
    13441 aattttacag gaaattactt tagaggatgt ccttgatgct ggacatcatt taatagatga
    13501 tggtgacata gttgatttta caatatttcc atcgtagtaa cctatcataa tagacactag
    13561 aaagaaggga tgacaagtat gagaaaaaaa acaattggag aggttttacg attagctaga
    13621 atcaatcagg gattgagttt agatgaattg cagaaaaaga cagaaatcca gttagatatg
    13681 ttggaagcaa tggaagcaga cgatttcgat caacttccaa gtccttttta cacgcgttct
    13741 ttcttgaaaa aatatgcatg ggctgttgag ttagatgacc aaattgtttt ggatgcttat
    13801 gattctggga gtatgattac ttatgaggaa gtagatgttg atgaagatga gttgacaggt
    13861 cgtagacgtt caagtaagaa aaagaagaaa aaaacatcat ttttaccttt attttatttt
    13921 atcctttttg ctttatcgat tttaattttt gtgacttatt atgtttggaa ctatattcaa
    13981 actcaaccag aggagccttc tctttctaat tacagtgtgg ttcaatcaac aagttcaact
    14041 agctctgttc cccactcctc aagtagtagt tcttctagta tagaatcagc tataagtgta
    14101 tcaggcgaag gaaatcatgt agaaatcgct tataagacaa gtaaggaaac agttaaattg
    14161 caattggcag tttcagatgt tacaagttgg gtcagtgttt cagaaagcga acttgagggc
    14221 ggtgtaacct tatcgccaaa gaagaaaagt gcagaagcaa cagttgcaac taaaagtcct
    14281 gtaacaatta cgttaggtgt tgtaaaaggt gttgatttga cagtagataa tcagactgtt
    14341 gatttatcga aattaacagc tcagactgga caaatcactg taacctttac taaaaattaa
    14401 ggaaaaacga atgaaaaaag aacaaattcc caatctctta acaataggtc gaattctctt
    14461 tatacctatt tttatcttta ttttaacgat aggaaattcg atagagagtc atatagttgc
    14521 agctattatc tttgctgttg ccagtattac cgactattta gatggatatt tagctcgtaa
    14581 atggaatgtg gtcagtaatt ttggtaaatt tgcagatcct atggcggata agttactagt
    14641 tatgtcggct tttattatgt tgattgagtt aggtatggct ccggcttgga ttgttgcagt
    14701 gattatctgt cgtgagttag ctgtgacagg tttaagactt ttattggttg aaactggtgg
    14761 aacaatttta gcagcagcaa tgcctggaaa aattaaaact tttagtcaga tgtttgccat
    14821 tattttcttg ctattacatt ggactttgct tggtcaagtt ctactttatg tagccttatt
    14881 tttcactatc tactctggct atgactattt caagggtagt gcctatgtat ttaaagggac
    14941 atttggttcg aaatgaaatc aataattgat gtaaaaaatc tttcttttcg ctataaagaa
    15001 aatcagaact actacgatgt gaaggatatt acgtttcacg tgaaacgtgg agaatggctt
    15061 tcgattgtag ggcataatgg tagtggtaaa tcaacgacgg ttcgattaat tgatggctta
    15121 ctggaagcag aatccggaga gattgtaatt gatggccaac gtttgactga ggaaaatgtt
    15181 tggaatatac gtcgtaaaat cggtatggtt tttcaaaatc cagacaatca atttgttgga
    15241 gcgactgttg aagatgatgt tgcttttggt ttggaaaatc agggactttc tcgtcaagaa
    15301 atgaaaaaga gagtggaaga agctctggct ttagttggca tgttggactt taaaaagaga
    15361 gagccagcgc gtctatcagg tggccaaaag caacgtgtgg ccattgcagg tgttgtagcc
    15421 ctaagaccag ctattttaat cttagatgaa gcaacgagta tgttggatcc tgaggggcgt
    15481 agagaactta ttgggacagt aaaaggaatt cgaaaagact atgatatgac ggtcatttct
    15541 attacccatg atttggaaga agtcgccatg agtgatcgcg tattggtcat gaaaaaaggg
    15601 gaaattgaat caactagtag tccaagggag cttttctctc gaaatgattt agatcaaatt
    15661 ggattagacg atccttttgc taatcaatta aaaaaatctt tgagccagaa tggctataat
    15721 ttacctgaaa attatttgac agaaagtgag ctagaggata agctatggga attgctctag
    15781 aaaatgtgaa ttttacatat caagaaggta ctcccttagc ttcagcagct ttgtcggatg
    15841 tttctttgac gattgaagat ggctcttata cagctttaat tgggcacaca ggtagtggta
    15901 aatcaactat tttacaactc ttaaatggtt tattggtgcc aagtcaaggg agtgtgaggg
    15961 tttttgatac cttaatcacc tcgacttcta aaaataaaga tattcgtcaa attagaaaac
    16021 aggttggctt ggtatttcag tttgctgaaa atcagatttt tgaagaaacg gttttgaagg
    16081 acgttgcttt tggaccgcaa aattttggag tttctgaaga agatgctgtg aagactgcgc
    16141 gtgagaaact ggctctggtt ggaattgatg aatcactttt tgatcgtagt ccgtttgagc
    16201 tgtcaggggg acaaatgaga cgtgttgcca ttgcaggcat acttgccatg gagccagcta
    16261 tattagtctt agatgagcca acagctggtc tagatcctct agggagaaaa gagttgatga
    16321 ccctgttcaa aaaactccac cagtcaggga tgaccatcgt cttggtaacg catttgatgg
    16381 atgatgttgc tgaatatgcg aatcaagtct atgtaatgga aaagggacgt ttagtaaagg
    16441 ggggcaaacc aagtgatgtc tttcaagacg tggtttttat ggaagaagtt cagttgggag
    16501 tacctaaaat tacggccttt tgtaaacgat tggctgatag aggcgtgtca tttaaacgat
    16561 taccgattaa gatagaggag ttcaaggagt cgctaaatgg atagtatgat tttagggcgt
    16621 tatatcccag gggattcgat tgttcaccga ttggatccac gtagcaaatt gcttgctatg
    16681 atgctactga ttttgattgt gttttgggct aataacccct tgacgaatct gattcttttt
    16741 atagcgacag ggatatttat tgccttgtca ggagtatctc tttcattttt tattcagggc
    16801 ttgaagtcta tgtttttctt gattgccttc acaactattt ttcaactatt tttcatttct
    16861 aatgggaatg ttttatttga gttttcgttt gtgagaatca cggattatgc tttgcaacaa
    16921 gctgggatta ttttttgtcg ctttgtattg attattttct tttcaacttt gttaacctta
    16981 acaaccatgc ctttaagttt ggcatcagct gtcgaagctt tattagcacc tttaaagcgt
    17041 gtgaaagttc cagttcatga aattggattg atgctgtcta tgagtttgcg ttttgtccca
    17101 accttgatgg atgatacgac gcggattatg aatgcacaga aagctcgtgg agtggatttt
    17161 ggagaaggaa gtatcgttca aaaagtaaag gcgatgattc ccattttgat tcctcttttt
    17221 gcgacaagtt taaaacgtgc agattccttg gctatcgcta tggaagcgcg tggctatcag
    17281 ggtggaaaag gcagaagtca ataccgacaa ttgaaatgga ctctaaagga tacgctgacc
    17341 attcttgtta ttctcgtact tggttgttgt ttatttttct taaaatctta gtagctttca
    17401 ggaattgata aaaagttact gtaacagttt ttgatataaa ggtagggata tgaaccgttt
    17461 taaaaaatca aaatatgtca ttattgtttt tgtcactgtt ctgcttgtgt cagctctctt
    17521 agcgacgact tattcaagta caattgtgac aaaattagga gatggaatct cattggttga
    17581 tagagttgta caaaaacctt ttcagtggtt tgattctgtc aaatcagatt tggctcattt
    17641 gacacgaaca tataatgaaa atgaaagttt gaagaaacag ctttaccaat tagaagttaa
    17701 atcaaatgag gtggaaagtt taaagacaga aaatgaacaa ctgcgccaat tgcttgatat
    17761 gaagtctaaa ttgcaagcca caaagacttt agcagcagat gttattatgc gttctccggt
    17821 atcttggaag caggagttga ccttagatgc aggtagatca aaaggtgctt ttgagaacat
    17881 gttagctatt gcaaatggtg gcttgattgg gagtgtttca aaagtagagg agaactctac
    17941 tatagtcaac cttctgacaa atacggaaaa tgctgataag atttctgtta aaattcaaca
    18001 tggctctact acaatttatg gaattattat tggctatgac aaggaaaatg acgttcttaa
    18061 aattagccaa ttaaatagta atagcgatat tagtgcggga gataaggtga ctactggtgg
    18121 attaggaaac tttaacgttg ctgatattcc tgttggtgaa gtggttgcca caacgcatag
    18181 tacagactat ttgacacgag aagtaactgt taaattgagt gcagatactc ataatgtaga
    18241 tgtgatagaa ttagtgggga attcataatg agacagttga agcgagttgg agtattttta
    18301 ttgcttcctt tctttgttct aattgacgcc catattagcc agcttctggg ctcatttttc
    18361 ccccatgtac atttggctag tcattttctt tttctatttc tcttatttga gacgatagaa
    18421 gtatcagagt atctctacct agtctattgt tttgttatag gcttggttta tgatgtttac
    18481 tttttccatc taatagggat tacaactctc ttatttatct tattgggagc cttccttcat
    18541 aaattgaata gtgttatttt gttgaatcgt tggacaagaa tgctagctat gattgtgctg
    18601 acattcctgt ttgaaatggg tagttatctt ttggctttta tggtagggtt gacagtagat
    18661 agcatgtcga tttttatagt ctatagcttg gtaccgacga tgattttaaa ttttttatgg
    18721 attactgttt ttcaatttat ttttgaaaaa tattatctat aagaacgaca tataaatgta
    18781 acaaaggcgt aatatttatt aggccttttt ttggtatact agtattgtct ttaaaagaag
    18841 gagtatctac gtaatatgaa gaaaaaaatc ttagcgtcac ttttattaag tacagtaatg
    18901 gtttctcaag tagctgtttt aacaactgcg catgcagaaa cgactgatga caaaattgct
    18961 gctcaagata ataaaattag taacttaaca gcacaacaac aagaagccca aaaacaagtt
    19021 gaccaaattc aggagcaagt atcagctatt caagctgagc agtctaactt gcaagctgaa
    19081 aatgatagat tacaagcaga atctaagaaa ctcgagggtg agattacaga actttctaaa
    19141 aacattgttt ctcgtaacca atcgttggaa aaacaagctc gtagtgctca aacaaatgga
    19201 gccgtaacta gctatatcaa taccattgta aactcaaaat caattacaga agctatttca
    19261 cgtgttgctg caatgagtga aatcgtatct gcaaacaaca aaatgttaga acaacaaaag
    19321 gcagataaaa aagctatttc tgaaaaacaa gtagcaaata atgatgctat caatactgta
    19381 attgctaatc aacaaaaatt ggctgatgat gctcaagcat tgactacgaa acaggcagaa
    19441 ctaaaagctg ctgaattaag tcttgctgct gagaaagcga cagctgaagg ggaaaaagca
    19501 agtctattag agcaaaaagc agcagctgag gcagaggctc gtgcagctgc ggtagcagaa
    19561 gcagcttata aagaaaaacg agctagccaa caacaatcag tacttgcttc agcaaacact
    19621 aacttaacag ctcaagtgca agcagtatat gaatctgcag cagcacctgt ccgtgcaaaa
    19681 gttcgtccaa catacagtac aaacgcttca agttatccaa ttggagaatg tacatgggga
    19741 gtaaaaacat tggcaccttg ggctggagac tactggggta atggagcaca gtgggctaca
    19801 agtgcagcag cagcaggttt ccgtacaggt tcaacacctc aagttggagc aattgcatgt
    19861 tggaatgatg gtggatatgg tcacgtagcg gttgttacag ctgttgaatc aacaacacgt
    19921 atccaagtat cagaatcaaa ttatgcaggt aatcgtacaa ttggaaatca ccgtggatgg
    19981 ttcaatccaa caacaacttc tgaaggtttt gttacatata tttatgcaga ttaatttaca
    20041 gagggactcg aatagagccc tcttttcagg ttttaccgtg acaatcccta ttaaaaatta
    20101 tatcaaaata gcttgaaaat attggaaaag tatggtagaa tgaaaattgt cgtgtgaacg
    20161 ataatactca ttcttgatga attgtgaagc agttgccctt gggtcgtttt gcgagttgaa
    20221 gtcaagaaga ggaaaaaaac aaaaaggaga aatactcatg gcagtaattt caatgaaaca
    20281 acttcttgag gctggtgtac actttggtca ccaaactcgt cgctggaatc ctaagatggc
    20341 taagtacatc tttactgaac gtaacggaat ccacgttatc gacttgcaac aaactgtaaa
    20401 atacgctgac caagcatacg acttcatgcg tgatgcagca gctaacgatg cagttgtatt
    20461 gttcgttggt actaagaaac aagcagctga tgcagttgct gaagaagcag tacgttcagg
    20521 tcaatacttc atcaaccacc gttggttggg tggaactctt acaaactggg gaacaatcca
    20581 aaaacgtatc gctcgtttga aagaaattaa acgtatggaa gaagatggaa ctttcgaagt
    20641 tcttcctaag aaagaagttg cacttcttaa caaacaacgt gcgcgtcttg aaaaattctt
    20701 gggcggtatc gaagatatgc ctcgtatccc agatgtgatg tacgtagttg acccacataa
    20761 agagcaaatc gctgttaaag aagctaaaaa attgggaatc ccagttgtag cgatggttga
    20821 caccaatagt gatccagatg atatcgatgt aatcatccca gctaacgatg acgctatccg
    20881 tgctgttaaa ttgatcacag ctaaattggc tgacgctatt atcgaaggac gtcaaggtga
    20941 ggatgcagta gcagttgaag cagaatttgc agcttcagaa actcaagcag attcaattga
    21001 agaaatcgtt gaagttgtag aaggtgacaa cgcttaattt atacaaatag taattaccta
    21061 ggagggcggg gcttagcccg gctctcctat tttcaaaaat ataggagaat taaaatggca
    21121 gaaattacag ctaaacttgt aaaagagttg cgtgaaaaat ctggtgccgg tgttatggac
    21181 gctaaaaaag cgcttgtaga aacagacggt gacatcgaaa aagcgattga attgcttcgt
    21241 gaaaaaggta tggctaaggc agctaagaaa gctgaccgtg ttgctgcaga aggtttgact
    21301 ggtgtttatg ttaacggtaa tgttgcagca gttattgaag taaacgctga aactgacttc
    21361 gttgcaaaaa acgctcaatt cgttgaattg gtaaatacta cagctaaagt cattgctgaa
    21421 ggaaaacctg ctaacaatga agaagctctt gctttgataa tgccttcagg tgaaactctt
    21481 gaagctgcat acgtatctgc aacagcaact atcggagaga aaatctcatt ccgtcgcttt
    21541 gcattgattg aaaaaacaga cgcacaacac tttggagcat accaacataa cggtggacgt
    21601 atcggtgtta tttcagttgt tgaaggtgga gacgaagcac ttgctaaaca attgtcaatg
    21661 cacatcgcag cgatgaaacc aacagttctt tcttacaaag aattggatga gcaattcgtt
    21721 aaagatgagt tggcacaatt gaatcacgtt atcgaccaag acaacgaaag ccgtgcaatg
    21781 gttaataaac cagctcttcc acacttgaag tatggatcaa aagctcaatt aactgatgat
    21841 gttattgctc aagctgaagc tgacatcaaa gctgaattgg ctgcagaagg caaaccagaa
    21901 aaaatctggg acaaaattat cccaggtaaa atggatcgct tcatgcttga taatactaaa
    21961 gttgaccaag cttacacact tcttgcacaa gtttacatca tggatgacag caagacagtt
    22021 gaagcatacc ttgaatcagt taacgcttcg gtagttgagt ttgctcgctt tgaagttggt
    22081 gaaggtatcg agaaagctgc aaacgacttc gaagctgaag ttgcagctac aatggcagca
    22141 gccttgaata actaataata gaaaggaagc aatttgcttc cttttttgta tagggcttat
    22201 atttttcctg aaattgtttt tcccccagtc tcactcgatt agaataatta aaacaaaaag
    22261 ccctataata agggctaagt gcatttagga gactatactt caaattcata gtaagcgcgt
    22321 cataacaagg tatctatcct aattccccaa cttccagtat actagataaa aaataattat
    22381 cggaggaaag gtattgtact atgatgattc aactaagtga tttaggtcaa gttcacattg
    22441 tttgtggcaa gacagatatg aggcagggaa tagactcatt agcctatgta gttaaaaccc
    22501 actttgaatt ggattctttc tccggtcaag tctttctctt ttgtggtgaa cgtaaagacc
    22561 gctttaaagc cctttactgg gatggtcaag gattttggct actatataaa cgctttgaga
    22621 acggaaaact gacttggcta agtacagaaa aggatgtcaa agctctcgca cctgaacaag
    22681 tagattggct tatgaagggc ttttctatca ctccaaaaat atagtagatt gaaactagaa
    22741 tagtacacct ctgcttctaa aacattgtta gaaatcgatt tga
//
LOCUS       DAVKJD010000023        22477 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKJD010000023 DAVKJD010000000
VERSION     DAVKJD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.023"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            42..746
                     /locus_tag="VW157_000966"
     CDS             42..746
                     /locus_tag="VW157_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9552288.1"
                     /translation="MGIKNSRGKYISFLDDDDEYMPDRILKLMACFKKSRMKNLALVY
                     SYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEVLELINGFEKIDSHQ
                     DGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINADEEYYNYCRKYFNL
                     LSFNERILVTKKYYSLNIKRLLLIGDKCKALKVIKKAREEKIFNEFLFLKYMLLYYFG
                     FFYCIYDNYVQLKFRK"
     gene            756..2372
                     /locus_tag="VW157_000967"
     CDS             756..2372
                     /locus_tag="VW157_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552289.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            2633..3061
                     /locus_tag="VW157_000968"
     CDS             2633..3061
                     /locus_tag="VW157_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552290.1"
                     /translation="MSEIVEMSKKISDLNDKKEFHKKNCELRFQDSVLDNINSVPGTT
                     HLFSCLNIQTLVKIILYIFRYNSCMKIFNFRDKELIFFNKIVNGLIQNIEENLEDDIE
                     RILKYLYICLFNEIFIIKNKVNFFDDVEFNQTLSEFLDKL"
     gene            3073..3822
                     /locus_tag="VW157_000969"
                     /pseudo
     CDS             3073..3822
                     /locus_tag="VW157_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
     gene            3835..5006
                     /locus_tag="VW157_000970"
                     /pseudo
     CDS             3835..5006
                     /locus_tag="VW157_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            5401..6264
                     /locus_tag="VW157_000971"
     CDS             5401..6264
                     /locus_tag="VW157_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9552291.1"
                     /translation="MIERMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLTSLFE
                     LRFQQYKELID"
     gene            6555..6752
                     /locus_tag="VW157_000972"
     CDS             6555..6752
                     /locus_tag="VW157_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU9552292.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            6820..7485
                     /locus_tag="VW157_000973"
     CDS             6820..7485
                     /locus_tag="VW157_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9552293.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            7557..8783
                     /locus_tag="VW157_000974"
     CDS             7557..8783
                     /locus_tag="VW157_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9552294.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(9180..9257)
                     /locus_tag="VW157_000975"
                     /pseudo
     CDS             complement(9180..9257)
                     /locus_tag="VW157_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            9328..9983
                     /locus_tag="VW157_000976"
                     /pseudo
     CDS             9328..9983
                     /locus_tag="VW157_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            9973..10296
                     /locus_tag="VW157_000977"
     CDS             9973..10296
                     /locus_tag="VW157_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9552295.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            10400..11230
                     /locus_tag="VW157_000978"
     CDS             10400..11230
                     /locus_tag="VW157_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9552296.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            11384..12238
                     /locus_tag="VW157_000979"
     CDS             11384..12238
                     /locus_tag="VW157_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9552297.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVVIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAIPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEETS
                     DGLDQPVW"
     gene            12338..13711
                     /locus_tag="VW157_000980"
     CDS             12338..13711
                     /locus_tag="VW157_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9552298.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREIALLETYGQRNPEE
                     VSRIYGLELPLLQEDRMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            13704..14765
                     /locus_tag="VW157_000981"
     CDS             13704..14765
                     /locus_tag="VW157_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9552299.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            14767..15459
                     /locus_tag="VW157_000982"
     CDS             14767..15459
                     /locus_tag="VW157_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552300.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(15489..16058)
                     /locus_tag="VW157_000983"
     CDS             complement(15489..16058)
                     /locus_tag="VW157_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9552301.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIAFFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVTPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            16193..16807
                     /locus_tag="VW157_000984"
     CDS             16193..16807
                     /locus_tag="VW157_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9552302.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            16809..18455
                     /locus_tag="VW157_000985"
     CDS             16809..18455
                     /locus_tag="VW157_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9552303.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHMGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            18467..19753
                     /locus_tag="VW157_000986"
     CDS             18467..19753
                     /locus_tag="VW157_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9552304.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     VCYAVGYSNLGYFYKVFRKLCGKSPKSYRKQVETIL"
     gene            19805..20338
                     /locus_tag="VW157_000987"
     CDS             19805..20338
                     /locus_tag="VW157_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552305.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            20416..20886
                     /gene="nrdI"
                     /locus_tag="VW157_000988"
     CDS             20416..20886
                     /gene="nrdI"
                     /locus_tag="VW157_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9552306.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(21179..22441)
                     /locus_tag="VW157_000989"
     CDS             complement(21179..22441)
                     /locus_tag="VW157_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9552307.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         6860 a         3806 c         4768 g         7043 t
ORIGIN      
        1 ttttaagtaa tgaagattta ggtgcaacta atgccagaaa cataggtata aaaaattcta
       61 gaggtaagta tatatcattt ttagacgatg atgatgaata tatgccagat cgaattttaa
      121 agttgatggc ttgttttaaa aagagtagaa tgaagaattt agctttagtt tatagttatg
      181 gaataataat ttatccaaat ggtacacgag aagaggagaa gaccgatttt gttggaaatc
      241 ccttgtttgt tcaaatggtt cacaatatag caggtacgtc attttggttg tgtaaaaaag
      301 aggtgctaga attaattaat ggttttgaga aaatagattc acatcaggac ggtgttgttt
      361 tattaaaact acttgctcaa ggataccaaa ttgatatagt gcgagaattc ttggtgaatt
      421 actacgctca cagtaaagaa aacggtatca ctggagtgac acaaaaaaca attaatgcag
      481 atgaagaata ttataattac tgtaggaaat attttaattt attgagtttc aacgagagaa
      541 tattggttac aaagaaatat tattctttaa acataaagcg gttactatta ataggagaca
      601 aatgcaaggc tttaaaagta atcaagaagg caagagaaga aaaaattttt aatgaatttc
      661 tttttttgaa atacatgtta ttatattatt ttggtttttt ctattgtata tatgacaact
      721 atgttcaatt aaaatttaga aagtgagaaa ctattgtgta tactattata aaatcaaata
      781 taaaaaaatt tagtttatta acgatattta ttgttgctgg tcaattattg ctaatttatg
      841 cagcaactat taatgctctg gtgttgaatg aattaattgc gatgaattta gagcggtttt
      901 tgaaattgtc aatctaccaa atgattgtct ggtgtgggat aatattcctt gactgggtag
      961 tgaaaaatta tcaggttgaa gtgatccaag agtttaatct agagattcga aatagagttg
     1021 ccacagacat ctctaactct acctatcaag aatttcatag taaatcatca ggaacatatc
     1081 tttcgtggct aaataatgat gttcagactt taaatgatca ggcttttaaa caactttttt
     1141 tagtaataaa aggaatttct ggtactatat ttgcagttgt gactcttaat cactatcatt
     1201 ggtcattgac tgtagccacc ttgttttcat taatgattat gctacttgta ccaaaaatct
     1261 ttgcatcgaa aatgcgagaa gttagtctaa atttaactaa ccaaaatgaa gcttttttaa
     1321 aatctagtga gactatattg aatggatttg atgtgttagc gtccttgaat cttttatatg
     1381 tattgcctaa gaaaattaaa gaagcaggaa ttttattaaa gatggttata caaagaaaga
     1441 caactgtaga aacgttagca ggcgctatta gcttctttct caatattttt tttcagatat
     1501 ctctcgtttt tttaacaggc tatcttgcaa taaaaggaat agtgaaaatt ggtactattg
     1561 aagcaatagg agcactaaca ggtgttattt ttacagcgct aggtgaatta ggaggtcaat
     1621 tatcctctat tattggtacg aagcctattt ttttaaaatt gtattcaatt aatccaattg
     1681 agtcaaataa aatgaatgat atcgaaccaa atgaggtgaa tagagatttt ccgttatatg
     1741 aagcaaaaaa tatttgctat aagtatggag ataaagaaat attaaaaaac ttaaattttt
     1801 gttttcaacg taatgaaaag tatttaattt taggtgaaag tggaagcggg aaatctacat
     1861 tattaaaatt attgaatggc tttttgagag attatagtgg agaattgcga ttctgcgggg
     1921 atgatataaa aaaaacctcc tatttaaata tggtttcgaa tgttctatat gtagatcaaa
     1981 aagcttattt gtttgaaggt acgattagag ataatatttt attggaagaa aattatactg
     2041 atgaagaaat actacagtct ttagagcaag ttggtttgag tgtaaaagat tttcctaata
     2101 acattttaga ttattatgtt ggtgatgatg ggagattact gtcaggaggg cagaaacaaa
     2161 aaattacttt agctagaggg ctaattagaa ataagaaaat agtattaatt gacgagggaa
     2221 cttctgctat cgataggaga acttcgttag cgattgaacg taagatatta gatagagagg
     2281 atttgactgt cattattgtt acccatgctc cgcatccgga acttaaacaa tattttacta
     2341 agatatatca atttccaaag gattttattt aagaatatag gggagggaaa catgagattc
     2401 tattgtgaag cttatgaggt aattactgaa gagaaaaaag tatcattttt ttatgtagat
     2461 gaaaaaccct taaggtatct gttatttgac tatatatttc tactagttct agctaaaaaa
     2521 aaatatccca atcttatgaa ctgcacccca aaagttagat ttattctgtc taacttttgg
     2581 ggtgcagttc attttgcata gttttggcat tcatcctaga tatccatata aaatgtctga
     2641 gattgttgaa atgtctaaaa aaatatcaga tctaaacgat aaaaaagaat ttcacaaaaa
     2701 gaactgtgag ttgaggtttc aagatagtgt tcttgataac ataaatagtg tcccgggtac
     2761 aactcattta tttagttgtt taaatattca aacattagta aaaattattt tatatatttt
     2821 tcgatacaac agttgtatga aaatatttaa ttttagagat aaagagctta tcttttttaa
     2881 taagattgta aatgggctga tacaaaatat tgaggagaat ttagaggatg atattgaaag
     2941 gatattgaaa tatttatata tatgtttatt taacgaaata ttcattatta agaataaggt
     3001 gaattttttt gatgatgtgg aatttaatca gacacttagt gagtttttag ataaattgta
     3061 gagggaatgg ttatgagtaa caatactaag tatatctttt tatctccaca tttagatgac
     3121 gcaatttttt cctgtggtga ttatatttca aaattaactt cagaaggaga aattgtactt
     3181 gttattacta tattttctgg ttaccctttg agctaacagc tacagccctc tgctaagcaa
     3241 tttcataagt tgtgtaacct tgggaaatat ccgattgaag aaaggaagaa agaagacagg
     3301 ttagcttgtg aacgtctaca atgtgatttt agacacttat cgtactatga atgtctctat
     3361 aggaaagata gaaatggcaa ttttctatac cgtcatatct atagtgaatt aaaaaatgag
     3421 gacacattaa agaatgatat cataaaagag ctgttaatgc atttagacga caaatgtgtc
     3481 gtatattgtc cgctttcact tggtgaccat gttgctcatg tttttgtgaa tagtattgga
     3541 agggcgcttg aatttatgcg ttataaagta atatattatg aggacttccc atatgtaagt
     3601 gatagtagta tggttagtta tatgggaaaa actaaagaat taaaaatgta tcaagaggaa
     3661 cttgatgaaa aaaattatat tgatagaata tcatcaatat tgtgctataa atctcagatt
     3721 ttgataatct ggaaatcagt tgaaaaactt ttaaacaata ttaaagagtt gtatttaagg
     3781 aatggggcag cttattctat tagattttgg attaagaaat aaggaagtag ttttatgaat
     3841 atagcagtaa ttggtttggg gcatgttggg ctggcctacg cgttactatt tgcatctaaa
     3901 tataaagttg ttgcatatga tatagattct gtaaaaataa ataatttaaa aaagggcatt
     3961 cttccatcta aaaatgaaga gcttatgaag tttttttgcg agaataactt aaatattact
     4021 ttttttgata cattttctga aattaaaaat aatattgatt attatattat tgcgcttccg
     4081 acagattatg atgagaaaat tggtagtttt aatacatatg aaatcgaaca aacggtatcg
     4141 aagattctga gggtaaaacc taatggaaag attattttaa agtcaacagt tccgatcggc
     4201 ttttcaaaca aattaaaaag gctgtttgat acaaaaaata tcatttttgt ccctgaattt
     4261 ttgagagaag gttgttctat atatgataat ttatatccaa gtcgcatagt tgttggagat
     4321 gagacagttg aaggaagaaa aattgcagag ttgttccttt cgattagtac tcatagtact
     4381 gccaatatta aaaatgttat gttagtttct cctactgaag cagaagcaat taagcttttt
     4441 tctaacacat tcttagctct ccgtgttgct tttttaatga actagattct tttgctgaga
     4501 ggagaagctt aaatgctgaa gttgtaataa aaggtgtttg tttagatcca agaattggaa
     4561 atttttataa taatccttct tttggatttg gaggatattg tcttcccaaa gatactaaac
     4621 aattaaaaaa agaatttata gaaataaatg ccccagtgat agaagcgatt gatatttcaa
     4681 acacaaatag aaaacagttc atagttaaac aaatattgga acgaaagcca aagatagtag
     4741 gaatatataa attggggatg aaatataatt cagataatta caaagagtca gctattttaa
     4801 gtataattaa tgaactattg attgtcggta taaaaatttt agtatatgaa ccaaatttaa
     4861 acgttagtat agataacgta attattgaaa agaattttga actatttaca aaacaaagtg
     4921 atctaattgt tgcaaataga tgggataggg gccttgaagc ttataaagat aaagtttaca
     4981 caagggggat ctggataaga gattaactat tgatattaaa aaggcaataa tttataaaat
     5041 ttgtacaaaa aaatgttaaa ttttcacatt ttcatagtat actgtttaat agaaagtagg
     5101 ttctttgtaa ccgctccatt ataaaatcct ttcactcttc cggtatacta ttaggaaaaa
     5161 cataactgga ggatattttg agctcacacc tcatcgttcc agtggctgtt ccccaagctc
     5221 gtgtactgcc caaaaaagtt agacattcta tttagaacga ggattgagtt ctgtattgta
     5281 caaggctcgg tccttttaga gtcagcttaa ggctggcttt tttgccctct taaatatcag
     5341 aatattttga caaatgattg taaataatga tataatatgc aaaagctagg aggtggtcag
     5401 atgattgaga gaatggaact gggggaattt tacaaggaat tacgtttggc gagaaagctt
     5461 aagcagacag atgtggcttg tgagggtcta acagcttctc agttgtccaa gtttgaacta
     5521 gggcagtcta tgctgtctgc ggataagttg attctagcta tccaaggtat aaatgtgacc
     5581 tttgatgagt ttgggcacaa gctcaacaac tatcaagaat ctccacatat gcgaatcggt
     5641 cgaaaggttg tgaatcgctt tgcccatcag gatatagctg ctttagagca gctattggag
     5701 gaagtcgatc aagaacagat ggcgcagacc tatcgtcgtt tgaatgccat cgtgattaaa
     5761 gatgccatcc attcactaaa taaaagctac ccactagcag aggaggatag cgagtttttg
     5821 accacctatc tctatgctat cgagtcttgg acctggtttg aactctatct cttttgcaat
     5881 accatgccct tcttgagcaa tcaagatctg atttttttat caacctcctt actcgaaaaa
     5941 tccaaagaat ttaaagagtt agtacacaat cgattgtata tgaagcaagg actcttaaat
     6001 atcttatcag aactcatgga gcgaaaactt ttctcctaca tcccaatctt cgaagccgag
     6061 ttggagagga tgttacgacc gtatgatgtt tttgaaaaag tatcgtggca atttttaaag
     6121 aagatgagtg tctttcttca aacaaaggga agcaatcaaa aagagattga acgctttatc
     6181 caatctctgc aggtattaga aaatccacaa ttaacatccc tttttgaatt gcgttttcag
     6241 caatataaag aacttataga ttagttaaaa atggaatgac aataacccac tccgaaataa
     6301 gtaggagtat cagttggatt tttcttttct tcatctatta tattcctcct tgttagtaat
     6361 aactttatta taccagggaa ataatcaaat ctatcaaaat cgcaaataag aaatttctat
     6421 aagaaaaaat atcaaatatg cgatttttta aaataagcca attttcgtgt tatactgtac
     6481 ttgtaaagca cttgaagcaa atcctaggtc gcagaagtgg tttacaaatg aagataattg
     6541 aaggagtgta aaagatgtta aatttacaat ttgcagaaac aatggaattg acagaagctg
     6601 agttggagac agtttatgga ggggaatttg ggaataatgc tgttatccca gccggtgctt
     6661 ggggaggttt aggaacgtct tggtcaatca ctaatttctg gaagaaatat tttaaccatg
     6721 attcttccac tgttaatcgt cgccattatt gatattcaga gtccttgcct ttttcttggg
     6781 tagggactgt ctttatttca tagtatagga gaaaatagaa tgaaaaagat aatctcacac
     6841 cgctacttta ttatagtttt tctactagtt attgctgacc agaagttcag tgttttagtt
     6901 ttacgcagcg accttgttgc tgggctatct gactttgcct attatctgtc ggatatgatg
     6961 ttgaattttc ttgtggtttt atttgctctt attgctatga tttggtcggg gaaatggcag
     7021 aaaatcaata gtagaaagtt taaaggatcc tatcttttct attccttcct agctctgctt
     7081 gcttttgttg cttggaattt tgtcaccttt tttcttttcc cacctactcg aaatgaaatt
     7141 tcttatcaac atgctgctcc tacttttaca ggagctacgg catttttgat gtattttttt
     7201 tatcctgtga ttgcaggtcc catttttgaa gacatgattt atcgtggatt agtgatgact
     7261 gctctggaaa aaggaaagaa atgggggcta gatgtacttg gttccgctgt tttatttggg
     7321 gtctcgcaca ttagtaatca cggttgggtc ttgacagact ttgtcttcta tatgggtgga
     7381 ggtcttatat ttgcagtctt atttagaatg acaaagtcaa tttattggcc tattggactg
     7441 catatagtct acaatggcat tggtcagctt ttgatgttac tgtaattttg gttgttagta
     7501 gtatcgcggt ctatccttgg ggaaagattc ccatttgaac ttaagaagga gttgtgatga
     7561 aagtatttct tcaaaataga gattttaggc aattaactat caaccagtgg atttcaacgc
     7621 ttggggatac gattttttat ctggcctttc tgaattatgt ggcagatgcg tcttttgcac
     7681 ctttggcgat tttactcatt acgatttcag aaacccttcc ccaaatttta caaatctttc
     7741 tgggagtttt ggcagatttt caacatcatc gtgtcttgaa atacacagtc attagttttg
     7801 caaaattttt gctttactct atcgtttctt tatcactttc agggcagtct ttctccttgt
     7861 tgctagtagc atttatttgt ctgcttaacc tcttgtctga cacattgagt tacttttcag
     7921 gagccatgct cactccgatt ttcattagaa ttattgggca agaccatctg gcagaagcta
     7981 ttggctttaa acagtcaact gttagtttag tgaaaacaat cagtaatatc ctaggaggag
     8041 tcttactagg cattctatcc atccagttta tttccttact gaacgctctt acctttttaa
     8101 tcgcattttt aggtatcctt ttcataaaaa ctgacctctt gaaagtagaa aaaacgatta
     8161 gctatcaaga aggactctct gtaaaatcct tttgccagca tttgctccaa tcatcgaaat
     8221 tgatatggaa tatgaataag gtgctcttgg ttttgtttat tatctctact agtcaagcag
     8281 tgataaatgt tacagttcct gtctctactc tatttttgag gaatcaaccc tttttgaatt
     8341 tacaaacagg tcaatctctt gccttgctat ccacttttga actgtcagcc ctgattgtcg
     8401 gaagccttgt aagtggctat ctgcaacata ccatttccat aaaaacagct ctctatgcct
     8461 cacttgtcat ccagttgctt cttttagtag gatttgccac agttcgtttt gactggattc
     8521 tcatctttag taccttggat gcctttttcg caggtgttct ctctcctaga ttgcaagaac
     8581 tcgtttttaa acaaatacct gaggagtcaa tgggagcagt tcaatcttct atcagtgcca
     8641 ttacggttgt tttacctagc ttatttacaa tagctttggt aaccattgct actagctttg
     8701 gaactctggc agttagcttt gttttattgc tatttcttct agttgccttt gtcatgcttc
     8761 tgaatattcg tgaaagtatt tagtggagaa tttctataat atatgtttga gactggattg
     8821 ccagtctttt tatgatggtc aaaattaaca gacttttttc tcttttctga atgtgttata
     8881 gtagactgaa tctaaaatag tacgaaacaa ttgctaaaaa atttatagaa attaatttta
     8941 ctttcccaat cgatttgttc tcatcttatt tcaatctgct atataatagt ccatgctgtg
     9001 gctggattct tttcagccta cttgtactct tcgaaactct cttcaaacca cgtcagcttc
     9061 actttgccgt aggtatggtt actgacttcg tcagttctat ccacaacctc aaaacagtgt
     9121 tttgagctga cttcatcagt tctatccaca acctcaaaac agtgttttga gctgacttca
     9181 tcagttctat ctgcaacctc aaaacagtgt tttgagcaac ctgcggctag tttcctagtt
     9241 tgctctttga ttttcattga gtattattaa gacaatatga ggagaaagat gaaagaaaaa
     9301 ggattttagg agggggcgca ggcagccatg ccaacggctc ttggctatgt cagtatcggc
     9361 ctggcctgtg ggactatcgg tgcgccctat gtgacacctg ttgagatggg cttgatgagt
     9421 ctctttgttt atgctgggag tgcccagttt gccatgttgg cactgattgt ggttcaagct
     9481 cctgtggcag ctattgctat gacggttttt ctaatcaact tgcgtctctt tttgttgagt
     9541 ttacacgcat cgacttattt ccgtcatacc agtctttggt acaatatcgg tatgtctagt
     9601 atcttgacag atgagaccta tggcgttttg atgggtgaat tggcccatac agacaaggta
     9661 aatcctatgt ggatgcacgg aaacaatctc aacagctatg tggcttggtt tgtggggaca
     9721 gtagtcggaa cggctctggg tggcctgcta ccaaatccag aaatctttgg cttggatttt
     9781 gccctggttg ggatgtttat tggtattttt gcttcgcaat ttcagattat gcaaagacgg
     9841 attcctgtcc gcaatctgct cattatccta gcagttgttg cggtgtcctt tttttgctct
     9901 tgacagtggt gtctcagtca ctagctgttc tgtttgcgac gctacttggt tgtagcatgg
     9961 gggtggtttt agatggtcag taagtatctt ttattagcag ttattttctc tggcttggtg
    10021 acttggattc tccgtatgat tcccttcatc ttagtcaagt ataagggctt gcctgcaatc
    10081 gttgagcgtt ttttgaagtt cttgcccgtt tccattatct ttgccttgat tctttcaagc
    10141 gtagtgacag gtaaggttgg gagccttcct caaattaaat ggctagactt cttagccgtc
    10201 tttccaacag cttgggtagc ctttcgctac cgcaatctag tcggaacagt tctctttgga
    10261 gtggtcttga ttgccatctt gcgtttggtc ttttaaatta cccataaaaa aaacttatca
    10321 cagagataga tatcatataa tggcgtaaat gctctttttc tgttaagatt ataaggtatt
    10381 ctattttgga ggaaatgaca tgaaaaaaat cgttaaatac tcatctcttg ctgccctagg
    10441 acttgttgct gcaggtgtgc ttgcggcttg ctcagggggt gctaagaaag aaggagaagc
    10501 agctagcaag aaagaaatca tcgttgcaac caatggatca ccaaggccat ttatctatga
    10561 agaaaatggc gaattgactg gttacgagat tgaagtcgtt cgcgctatct ttaaagattc
    10621 tgacaaatat gatgtcaagt ttgaaaagac agaatggtca ggtgtctttg ctggtcttga
    10681 cgctgaccgt tacaatatgg ctgtcaacaa tcttagctac actaaagaac gtgcggagaa
    10741 atacctctat gccgcaccaa ttgcccaaaa tcctaatgtc cttgtcgtga agaaagatga
    10801 ctctagtatc aagtctctcg atgatatcgg tggaaaatcg acggaagtcg ttcaagccac
    10861 tacatcagct aagcagttag aagcatacaa tgctgaacac acggacaacc caactatcct
    10921 taactatact aaggcagact tccaacaaat catggtacgt ttgagcgatg gacaatttga
    10981 ctataagatt tttgataaaa tcggtgttga aacagtgatc aagaaccaag gtttggacaa
    11041 cttgaaagtt atcgaacttc caagcgacca acaaccgtac gtttacccac ttcttgctca
    11101 aggtcaagat gagttgaaat catttgtaga caaacgcatc aaagaacttt ataaagatgg
    11161 aactcttgaa aaattgtcta aacaattctt cggagacact tatctaccgg cagaagctga
    11221 tattaaataa tttcttgaaa tcatccattc tgagtaaggt gggtgatttc tcagttttta
    11281 gggatatagt tttaaactat atatctgatt atctaataac aatttgtgaa atcaaacaaa
    11341 ttgtgagata ctagtacggt attattttta aggagaaaga atcatgaaaa tcaaaaaatg
    11401 gcttggccta gcagcccttg ctacagtcgc aggtttggct cttgcagctt gcggaaactc
    11461 agaaaagaaa gcagacaatg caacaactat caaaatcgca actgttaacc gtagcggttc
    11521 tgaagaaaaa cgttgggaca aaatccaaga attggttaaa aaagacggaa ttaccttgga
    11581 atttacagag ttcacagact actcacaacc aaacaaagca actgctgatg gcgaagtaga
    11641 tttgaacgct ttccaacact ataacttctt gaacaactgg aacaaagaaa acggaaaaga
    11701 ccttgtagtg attgcagata cttacatatc tccaatccgc ctttactcag gtttgaatgg
    11761 aagtgccaac aagtacacta aagtagaaga catcccagca aacggagaaa tcgctatacc
    11821 gaatgacgct acaaacgaaa gccgtgcgct ttatttgctt caatcagctg gcttgattaa
    11881 attggatgtt tctggaactg ctcttgcaac agttgccaac atcaaagaaa atccaaagaa
    11941 cttgaaaatc actgaattgg acgctagcca aacagctcgt tcattgtcat cagttgacgc
    12001 tgccgttgta aacaatacct tcgttacaga agcaaaattg gactacaaga aagcactttt
    12061 caaagaacaa gctgatgaaa actcaaaaca atggtacaac atcattgttg cgaaaaaaga
    12121 ttgggaaaca tcacctaagg ctgatgctat caagaaagta atcgcagctt accacacaga
    12181 tgacgtgaaa aaagttatcg aagaaacatc agacggtttg gatcaaccag tttggtaata
    12241 agaaacaggg aggtgggaga gagaattcca cctcttgctt ttgtatagag cacggagtgt
    12301 aaagaagact attcgttcat agaaaggtag agagaatatg gtttttccta gcgaacaaga
    12361 acagattgaa aaatttgaaa aggatcatgt agcccagcat tattttgagg ttttgcgtac
    12421 cttgatttct aagaaatcag tctttgccca gcaggttgga ctcaaggaag tcgcaaatta
    12481 tctgggtgag attttcaagc gtgttggagc tgaagtggag attgatgaga gctatacagc
    12541 gccctttgtc atggcacatt tcaagagttc gcgtccagat gccaagacct tgattttcta
    12601 taaccactat gacactgtgc cagcggatgg ggatcaggtc tggacagagg atccttttac
    12661 gctttcggtc cgcaatggct tcatgtatgg gcgtggggtt gatgacgaca agggtcatat
    12721 cacagctcgc ttgagtgctt tgagaaaata tatgcagcac catgatgatt tacctgtcaa
    12781 tatcagcttt atcatggagg gagcggagga atcggcttca acagacctag ataagtattt
    12841 ggaaaagcat gcagacaaac tccgtggggc ggatttgttg gtctgggaac aagggaccaa
    12901 aaatgccttg gaacagctgg aaatttctgg tggcaataag gggattgtga cctttgatgc
    12961 caaggtaaaa agcgctgatg tggatatcca ctcgagttat ggtggcgttg tggaatcagc
    13021 tccttggtat ctcctccaag ccttacagtc tcttcgtgct gcggatggcc gtatcttggt
    13081 tgaaggcttg tacgaagaag tacaagagcc caatgaacga gaaatagcct tgctagaaac
    13141 ttatggtcaa cgaaacccag aggaagttag tcggatttat ggattggagt tgcctctctt
    13201 acaggaggat cggatggcct ttctaaaacg tttctttttc gagccagcgc ttaatatcga
    13261 aggaatccag tctggttatc aaggtcaggg tgttaagact attttacctg cagaagccag
    13321 tgccaagcta gaggttcgtc tggttccggg cctagaaccg catgatgttc tggaaaaaat
    13381 tcggaaacag ctagacaaaa atggctttga taaggtagaa ttatactata ccttgggaga
    13441 gatgagctat cgaagcgata tgagcgcacc agccattctc aatgtgatcg agttggccaa
    13501 gaaattctat ccacagggcg tttcagtctt gccgacgaca gcggggacag gacctatgca
    13561 tacggtcttt gatgccctag aggtaccaat ggttgcattc ggtctaggaa atgccaatag
    13621 ccgagaccac ggtggagatg aaaatgtgcg aatcgctgat tattacaccc atatcgaatt
    13681 agtagaggag ctgattagaa gctatgagta gagatattat caagttagat cagatcgatg
    13741 tgacttttca ccaaaagaag agaaccatca cagcggttaa ggatgtgacc attcacatcc
    13801 aagaagggga tatctacgga atcgttggat attctggagc agggaaatca acccttgtac
    13861 gggtgattaa tctcttgcaa aaaccatctg cagggaaaat taccattgac gacgatgtga
    13921 tttttgatgg taaggtcacc ttgacggcag agcagttgcg tcgtaaacgt caagatatcg
    13981 ggatgatttt ccagcatttt aacctgatga gtcaaaagac agcagaggag aatgtagcct
    14041 ttgcccttaa acactctgga ctcagcaagg aagaaaagaa ggctaaagta gctaagttgt
    14101 tggacttggt tggtttggca gatcgtgctg aaaactaccc ttcacaacta tctggagggc
    14161 aaaaacagcg tgtggcaatt gcgcgtgcct tggccaatga tccaaaaatc ttgatttcag
    14221 acgagtcaac ttctgccctt gaccctaaga caaccaagca gattttggcc ttgttgcaag
    14281 atttgaacca aaaattagga ttgacagttg tcttgattac gcatgaaatg cagattgtca
    14341 aagacattgc caaccgtgtg gcggttatgc aggatgggca tttgattgaa gagggaagtg
    14401 tccttgaaat cttctcaaac cctaaacaac ctttgactca agactttatc tcaacagcca
    14461 caggtattga cgaagccatg gtcaaaatcg agaagcaaga aatcgtggaa cacttgtctg
    14521 aaaacagtct cttggtgcaa ctcaagtacg ctggagcttc aacagacgag ccacttttga
    14581 atgaattgta caagcattac caagtaatgg ctaatattct ctatgggaat atcgaaattc
    14641 tcgatggtac tcctgttgga gaattggtgg tggttttgtc aggtgaaaaa gcagcgttgg
    14701 caggtgccca agaagccatt cgtcaagcag gtgtacaact aaaagtattg aagggagtac
    14761 agtaagatgg aatcattgat tcaaacctat ttaccaaatg tctataaaat gggttgggct
    14821 ggtcaggcag gctggggaac ggctatctac ttaactcttt atatgacagt tctttccttc
    14881 attatcggag gcttcttggg gctagtggca ggtctctttc tcgtcttgac agcgccaggt
    14941 ggtgtcttgg agaataaagt cgtattctgg attttagaca aaattacctc aatttttcgt
    15001 gcggttccct ttatcatcct cttggcaatc ttgtcaccac tttctcactt gattgttaaa
    15061 acaagtatcg ggccaaatgc agcccttgtc ccactttctt ttgcagtctt tgccttcttt
    15121 gcccgtcagg tgcaggttgt cttggctgaa ttggatggcg gtgtcattga ggcggctcaa
    15181 gcgagcggag cgactttctg ggacatcgtg ggtgtttacc tatcagaagg tcttccagat
    15241 ttgatccgtg tgacgactgt gaccttgatt tcccttgttg gggaaacagc tatggccggt
    15301 gcggttggag ctggtggtat cggtaacgta gccatcgctt atggatttaa ccgctacaat
    15361 cacgatgtga ccatcttggc aaccatcatt atcattttga ttatctttgc aatccaattc
    15421 ttgggagatt tcttgactaa gaaattgagc cataaataaa aaagagccgt gtggctcttt
    15481 ttaactgatc agattttctg ggcaaatttt ttactcaagg cttgtccaat caaggcaccc
    15541 actagggctc cgatgacaat acttgcgata aatagaagga cagttccagg gtttggagtg
    15601 accatgatac ggtcgatata ttcttgggat tttcctcttg ccagaagagt agccatatag
    15661 gctttgggcg caatccacat aagcaagatt ggtcctgttg tactaaaggc gaaaataatg
    15721 aaagaaagga agttctttgt tttgtccttg tattttccta aatgagctac ttcatctgct
    15781 aggaggccac agataattcc aggaaggaag gaaccagcac cgtgtttagt tcccaagaaa
    15841 aagaaggcaa tgacaaggcc gatagtggta atggctccaa agcgcggaac ttttgcgact
    15901 aggatcatat agacgctacc gccgacaagg gcagtaaagg caggcgcata aaacatgttt
    15961 ccagtttggt caaagagatt gcccaaaagg acaccaatcc ccatgcagag gaagtaaaga
    16021 actgcaaaaa gcagtgtagt taagatagat tttttcatga attgtctcct gataattttt
    16081 tcacaattct cattgtacca cggtttggtg ggattgtaaa tggatactag aagtttttga
    16141 aaacgcttga aatatgatat aatggtttca tagttatttt taggggaata tcatgggtag
    16201 atttttagac tttgtcttta atcgtttctt tttagggatg attgcgacag ccttcttttg
    16261 gctattaact ttagcaggag ggattatcct tggtctagcg ccggctagtg ccaccttgat
    16321 gagcttatat gcagaacatg gttatagctt tcgggaatac agtttgaagg aggcttggtc
    16381 tctttacaag caaaattttg tctcaagcaa cctgattttc tatagctttt taggtgtggg
    16441 tctagttttg acctatggtt tgtatctctt ggtgcaattg cctcatcaga ccattgttca
    16501 tttgattgcg acccttttga atgtcctagt agttgccctg atctttttgg cttatacagt
    16561 atctttaaaa ttacaagttt attttgcctt gtcctatcga aatagtctca aattatcctt
    16621 gattggcatc tttatgagtc tagcagctgt ggccaaggtt ctccttggga ctgtgctact
    16681 tgtagcaatt ggttactata tgcctgccct gctatttttt gtaggaattg ggatgtggca
    16741 tttctttatc agtgatatgt tggaacctgt ctatgaaatc atccatgaaa aattggcgac
    16801 aaaatagaat gaagcagttt tggctacata cgcttctaag aacctatagt tcagtgatga
    16861 tcattatcat tgcgagtttt gcaatcttac tctcttacgc tgactgggat tcacgtgaaa
    16921 aggaagccca gagagtagcc cagcgtgtaa ctgctagaac agtgagtgaa attgaatatt
    16981 accatagaga gtcaacccag atagctcagg ctttagttga aaatcaagct cgtatcgagg
    17041 gaatctataa atactttagc cttagcatgc cagactattt ttactggcaa ttagagcgga
    17101 aagcttcgcc ttatatatca gtctctctgt atgaaaatgt tgatgacctc tatgttcgaa
    17161 atgattttgt aactggggtg gccattgctt ttcaagatta caaggaagtc tatgtttcta
    17221 ctaaagacaa acgtagtgga gaaaaaatca gggctgagga tttcaaacca gcaggaaata
    17281 gttttgccat tccagtgtca gatccagtgt cagatcaaga cttaggagtg atttacatct
    17341 ccttggatcc tgctgtttta taccatgcca ttgataatac tagaggtcat actccgatgg
    17401 cagtaacagt gacctcacct tttgatacgg agatttttca tatgggtgag acagttgata
    17461 aggagagtga aaattggcta gttggcttaa cttctcatgg ttatcaggtt caggtggcag
    17521 ttcccaaaaa ctttgtttta caaggaacgg tgaccagctc tgctttgatt gtgggcttga
    17581 gccttctctt tattgtcatt ctttatctga ctttgaggca gacttttgct aattaccaaa
    17641 agcaggtagt ggatttagtg gaatccattc aagtcattgc tcaaggcgaa gaggggcgtc
    17701 ggattgacat ttccgaaaaa gatcaggaat tactcctaat cgcggagacg accaatgata
    17761 tgttggatcg attggaaaag aatatccatg atatttacca gttagagctt agtcaaaaag
    17821 atgccaatat gcgagccttg caggcgcaaa tcaatcctca ttttatgtat aatacgctgg
    17881 agttcttgcg catgtatgca gttatgcaga gtcaagatga gttggcagat atcatttatg
    17941 aattcagtag tctcttgcgt aacaatattt ccgacgaaag agagaccctc ctcaaacagg
    18001 aattagaatt ttgccgtaaa tacagctatc tctgcatggt tcgctatccc aagtccattg
    18061 cctatggttt caagatagat ccagagttag agaatatgaa gattcccaag tttaccttgc
    18121 aaccgctggt agaaaactat ttcgcgcatg gtgttgacca caggcggaca gataatgtga
    18181 ttagcatcaa ggctcttaaa caggatgggt ttgtggaaat tttggtggtc gataatggta
    18241 gaggaatgtc ggctgaaaag ttggcaaata tccgagaaaa attaagtcag aaatattttg
    18301 aacaccaagc cagctacagt gatcaaaggc agtctatcgg gattgtcaat gtacacgagc
    18361 gttttgtgct ctattttgga gaccgctatg ccattactat agagtctgca gagcaagccg
    18421 gtgttcagta tcgtattaca attcaagatg agtagaaagg gagaaaatgt ataaagtatt
    18481 attagtagat gatgagtaca tggtgacaga aggtctgaag cgtttgattc cctttgataa
    18541 gtgggatatg gaggtcgtcg caacagccag tcatgccgat gaagctctag aatatgttca
    18601 ggaaaatcct gtcgatgtca tcatttccga tgtcaatatg ccagacaaaa cagggcttga
    18661 tatgattcgg gagatgaaag agatcttacc agatgctgcc tatatcctgc tctcaggtta
    18721 tcaggagttt gattatgtaa aaagagcaat gaaccttagt gtggtggact atttggtcaa
    18781 gcctgttgat aaggtagagc tgggaaatct gctggagaag attgcaggtc agctcggcga
    18841 gagagggaag aaaagtcaga ctcttagtca agaattagac gaggctggat ttgttagtta
    18901 tttaggggat aaggagaatt ggtggatagg tctatccaag gaaaaacaag gttccttcac
    18961 cattccctac tatgtcttgg gccaagactg gcagattttc atttctgacc aacccctaga
    19021 tggtttagtc gttacacctt ttgaagctcc ttatcaagaa cactttgaac gctggaagct
    19081 gaatgctgag aaaaccctct tttacggttc tgtaaatttg cagcagtctg agagtctctt
    19141 tgcctattac gaaccgattt atagggttat tattcaggga aatctcaatc aaatcgtaga
    19201 agagttaaat ctcttggaga aggtagttct tgaaaatacg ccgcgtgttc cgattactaa
    19261 acagcttttt attcagtttg tcatggatgt tttccattta tttgaacatc tcaaagctga
    19321 tgacctgaca gacatcgtca aaaccattca tgctattcaa tccttcgatg aattggttcc
    19381 ttatatcaag gaaactctga gcagcttttt cggtcaatac cgtatgaatg aaaatgtggt
    19441 cagtgtgctg gaagtcattg gtcgtgatta ccaaaaagag ctttccctca aggatatcag
    19501 taaggctctc tttatcaatc ctgtctatct agggcagttg attaagcgtg aaaccgattc
    19561 gacttttgca gagttactaa acaaacaacg tattaaggct gcccaacaac ttttgctttc
    19621 aactagtgac agcatcgaag atgtttgtta tgctgttggt tacagtaacc ttggatattt
    19681 ctataaagtt ttccgaaaat tgtgcggaaa atcgccaaaa tcctaccgaa aacaggtaga
    19741 aactatacta taagatttgt attcctttac aaaaggtgct ataatattat caggaggtcc
    19801 tatcatgaaa aagaaaccaa tttatctatg ggtcttgcta atcttgtctg cccttatttc
    19861 agttccgtct ctgtttggaa ttgtgagtcc cttgcctagt aaagaagccc ttcgtgctgc
    19921 tcagaaacaa gttgcagggg tcaatgctca gcaattagaa gaccagctta attataccta
    19981 tagagtagca gaagcatctc attctatttt taatgttgct ttgattgtgc tatctactat
    20041 tttagtggtc gtagcgattg tcttccttgt tcgtaaaaat ttgcaatatg caaactatac
    20101 ttacgttggc tatgttttgt tagctattat tggttcgatt tatggctatg ttggtttaca
    20161 agacgctgtg cagttggttc aagatgagag catgcgtttg acagtgagta ttggctcaaa
    20221 agctgttagc attttctata tcgtcatcaa tgttctcttc ctagcccttg tcttctataa
    20281 gatgtggcga caacagaaag ccttggcaga agaagaggaa acagaagagc ttacctaagt
    20341 attgacaaaa aagaactatc aagatacaat cttggtagtt gttttttgat tatagataaa
    20401 atcatggagg tacatatgaa gacaatttct ttagtttata ttagtttgag tggcaacact
    20461 gaaagttttg tgacgcgctt gaaagactat ctcttgtccc agtacaaagg gattgaggtt
    20521 caaaagattc atatcaagga tttggtcaag gaaggcaaaa atttctatga aatggaccat
    20581 ccctatgtcg cttttttgcc gacctatctc gaaggtggga atggcgtgga taacggagat
    20641 gttgagattt tgacgacacc ggtgggagat tttattgcct atggtaacaa tgctagtaag
    20701 tgttttggtg tggttgggtc aggaaatcgt aactttaata accaatactg cctaacagcc
    20761 aagcaataca gtcaacgctt tggcttccct gtattggctg actttgaaat gcgaggcatg
    20821 ctggaagata tcaaacatgt cgcagctatt atcgcagatt tgtatgagtt ggaaaaggag
    20881 aattaatact caatgaaaat caaagagcaa actaggaagc tagtcgcagg ttgctcaaag
    20941 cacagctttg aggttgcaga tagagctgac gtggtttgaa gagattctcg aatagtataa
    21001 agttcttata agaggtggtt gaagttgttc aactgttttt tgagtataaa cagtctttaa
    21061 gaacgtaaaa aagcataagt tcaggtcttc aaaaccttgt tcttatgctt tttatcatag
    21121 aaatatttgc tgaattttct cttgaaatga aaccttatct tcatctcgcg cttgtccctt
    21181 accctgcttt tccgattgcg agtgcgtaga taaagaagat ggcgaagcca aagaggaaaa
    21241 tcaatcccgc aatggcaagg tgtttgagcc aagaaggaag atttttgttt tcacgcgtta
    21301 ccaaggcgta attcaaaaga gcaaagaaag gtgttgtcag gaaagatcca atcatggcaa
    21361 agcggagcat ggttgaaacc tgaccagcga agaacttgat aatgacgata ccgatgatag
    21421 cagtgatggt catccagatg ttcaaagatt tacgattgtc ctctttttga ctgattagca
    21481 gtcggagaga ttcctgatta acgcgagaat agccatcgat aacagttata actgttccaa
    21541 agatacagag gaaggcaata aaggtaatca agtaacggga ccattcgcca agaacagagg
    21601 catacatgcc cacgaattga gagatgtatt tggctgaagc agcttcaacc gcctgccctg
    21661 taggatactg aatcagtgct cccagtgcca caaagaagac ggctaggata gctgttccaa
    21721 tataaccagt gttaaagtca aacagagcgt cttctgtgtt aaagttgacg gtctttctct
    21781 tttcagctga ccaaagtgaa ttgatggctg aaatttcaat aggagccggc atccatccta
    21841 ggagggagac gatgaagggc agagctgcca tttgccaagg tgtcttctcg acaaaatcag
    21901 aactgtattc tggatgcttg accgccgcaa tgataactgc cagaacagtc gcaatggtta
    21961 aggcagacat aatccatttg accatgccgt ctaaaagttt gtagcctcca aagagtagca
    22021 tagcccaaat gattgcaacg agaatgaggg accactgagt aatgctaagt ccaatcattg
    22081 ggaaggcact ggcgatgata gctgagcaca gaatggcaac accagccgtg ttgaccatag
    22141 ccgaaaagac attgaggata aagaaaatcc agagatagag ttttcctttt tcggcataac
    22201 cttcaaccaa agtctttcca gtatcagctg tgtattcagc accaaaacgg aaaaatggat
    22261 atttaaagac attggctaag atgaccaaga gaagtagaga ccaaccgtaa gaaccgccag
    22321 cttgagttga ggatacaatg tgggaacctc caacagcggc agttgccatt aggattccag
    22381 gtcccattgc ttttagcttg cttgcccagg ttgattgatt taaggaaata gcttgcgaca
    22441 tgataaatac tcctttttga attttcagaa taatctg
//
LOCUS       DAVKJD010000024        22280 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKJD010000024 DAVKJD010000000
VERSION     DAVKJD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.024"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(35..682)
                     /locus_tag="VW157_000990"
     CDS             complement(35..682)
                     /locus_tag="VW157_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEU9552308.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KRTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LELGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(791..1309)
                     /locus_tag="VW157_000991"
     CDS             complement(791..1309)
                     /locus_tag="VW157_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9552309.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1536..2405)
                     /gene="rsmI"
                     /locus_tag="VW157_000992"
     CDS             complement(1536..2405)
                     /gene="rsmI"
                     /locus_tag="VW157_000992"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9552310.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQILKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2408..2725)
                     /gene="yabA"
                     /locus_tag="VW157_000993"
     CDS             complement(2408..2725)
                     /gene="yabA"
                     /locus_tag="VW157_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9552311.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2765..3655)
                     /locus_tag="VW157_000994"
     CDS             complement(2765..3655)
                     /locus_tag="VW157_000994"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9552312.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQGEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWKANVSFQNTMEYLVLKEI"
     gene            complement(3652..4290)
                     /locus_tag="VW157_000995"
     CDS             complement(3652..4290)
                     /locus_tag="VW157_000995"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9552313.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMDLAK"
     gene            complement(4509..5306)
                     /gene="proC"
                     /locus_tag="VW157_000996"
     CDS             complement(4509..5306)
                     /gene="proC"
                     /locus_tag="VW157_000996"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9552314.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMDAVHQAYKRTQELGK"
     gene            complement(5310..6572)
                     /locus_tag="VW157_000997"
     CDS             complement(5310..6572)
                     /locus_tag="VW157_000997"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9552315.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQVLLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADDDKALSIINNAKTSRPSVCNAMEVLLVHEDKAASFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(6582..7712)
                     /locus_tag="VW157_000998"
     CDS             complement(6582..7712)
                     /locus_tag="VW157_000998"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9552316.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDEVKVGDNDTLSA
                     QVAAMVQADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(7792..9675)
                     /gene="cbpE"
                     /locus_tag="VW157_000999"
                     /gene_synonym="pce"
     CDS             complement(7792..9675)
                     /gene="cbpE"
                     /locus_tag="VW157_000999"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9552317.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMNIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNAKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(9678..10565)
                     /locus_tag="VW157_001000"
     CDS             complement(9678..10565)
                     /locus_tag="VW157_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9552318.1"
                     /translation="MEIKIETGGLRLDKALSDLTELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10555..11016)
                     /locus_tag="VW157_001001"
     CDS             complement(10555..11016)
                     /locus_tag="VW157_001001"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9552319.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRASQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(11013..11921)
                     /locus_tag="VW157_001002"
     CDS             complement(11013..11921)
                     /locus_tag="VW157_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9552320.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11914..12111
                     /locus_tag="VW157_001003"
     CDS             11914..12111
                     /locus_tag="VW157_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9552321.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(12163..12834)
                     /locus_tag="VW157_001004"
     CDS             complement(12163..12834)
                     /locus_tag="VW157_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9552322.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITVFWDVWLL
                     LFSGS"
     gene            complement(12888..13109)
                     /locus_tag="VW157_001005"
     CDS             complement(12888..13109)
                     /locus_tag="VW157_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU9552323.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            13381..14190
                     /locus_tag="VW157_001006"
     CDS             13381..14190
                     /locus_tag="VW157_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9552324.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHQAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKLRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14371..15246)
                     /gene="aguB"
                     /locus_tag="VW157_001007"
     CDS             complement(14371..15246)
                     /gene="aguB"
                     /locus_tag="VW157_001007"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9552325.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15256..16341)
                     /gene="aguA"
                     /locus_tag="VW157_001008"
     CDS             complement(15256..16341)
                     /gene="aguA"
                     /locus_tag="VW157_001008"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9552326.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEVLEKPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16338..17465)
                     /gene="nspC"
                     /locus_tag="VW157_001009"
     CDS             complement(16338..17465)
                     /gene="nspC"
                     /locus_tag="VW157_001009"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9552327.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17465..18724)
                     /locus_tag="VW157_001010"
     CDS             complement(17465..18724)
                     /locus_tag="VW157_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9552328.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(18721..19581)
                     /gene="speE"
                     /locus_tag="VW157_001011"
     CDS             complement(18721..19581)
                     /gene="speE"
                     /locus_tag="VW157_001011"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9552329.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAVGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            19838..20395
                     /locus_tag="VW157_001012"
     CDS             19838..20395
                     /locus_tag="VW157_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9552330.1"
                     /translation="MLYVGIDVAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALKLLSDPNREQFQHDNRQVDLKILARHIHRLKKKQSDWKVQYTRCLD
                     IIFPELDKIVGKHSEYTYQLLTRYPNTQKRIEAGFDKLIEIKRLTASNSRAFCRALKS
                     SPNHQIEIRNSLDKTIDFSYSLSFS"
     gene            complement(20361..21836)
                     /locus_tag="VW157_001013"
     CDS             complement(20361..21836)
                     /locus_tag="VW157_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9552331.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         6458 a         5110 c         3998 g         6714 t
ORIGIN      
        1 aatgaatttg agtgctggta ttatcaatca tgaactagga ttctagtttt gcaggaagtc
       61 taatgtaatt ctcaagctgt gagttcatta tgatttgtaa atccgatata tcttcagcag
      121 ctaaaaacac attttgaaat gttccaagtc cgttttcacc aaaactatat tctctcttac
      181 tatacttact cttattattc tctatatagt tagagtcaag gttcttaacc tcttgaagtt
      241 cattttcttt acttctgagg ttaaaatctt ttacttctga agttaagttt tttgacttct
      301 gtaagtcatt ttcttttacc tgaaatatac tcataaagtc tttaacataa atgatgttcg
      361 gcttaccaag ttcaagcctt actctttcga tcagtcctat tccttttttt acatcaagct
      421 catctaatgt ttttatggca gttggctttg agatatttct tcttttcata atttcttcga
      481 cagtaaaata gataaatact ctgccttcct tgtctatcca gttattctta aatgacattc
      541 ctgtccgttt aagaagtatg gaataaagga taattgcttc tgctgataag ccccgaaatt
      601 cttctctatc aactaatatc tccggtactt ttaagaagtt aaatctttcc gcttctctgt
      661 tataaaaata ttcaaagtcc atgcaagatc acctcccttc ttaaaaattt gcaaagaaaa
      721 agacgatagt ttttcttcta tcgcctttgg taaccatttt tatgaaattt tttaaagtta
      781 aaaatataat ttataatact atcttcttcc attttctgat tttagttctg aaagttccaa
      841 aaggtgcaac cgtatttaca tgaataaact tatacacttc ccaagtcgct gttttagtcg
      901 cttcatcagc ccactttctc atatgcggtt caaataattc ttcctcactc aacgaatcaa
      961 tcattgcaga gatagaatta atattttcat ttaattttgc tttcaactct tgcagagata
     1021 aatgagcgta ggtatctgtg aaccactgat ataattcacc aagttgattc catttaaatt
     1081 tatccgatgg tgtttttact tgaagcccct ttctttcatc ttcttcccat ttaagaacca
     1141 agttggtcca accaacctga taagcaaggt tttctgctgg agttctgtca acttcatcag
     1201 ctctcttatc ttttaaattt tctggaatat tatcaaattc taaaatatat ttctcaaatg
     1261 ttttctctat ctcagctttt agttcttctt tattttcgta tgttcgcaaa aaatcacctt
     1321 ctttctgatt tatcaatcct ttgatttaaa atgaccttat ttttctattt ataccttcta
     1381 agaacgctat aactgctgct ccaaccatcg gtattccgta tgggcttaat aaaaaactta
     1441 ataccaaagc ttctatacca agtgatacat ctttatatta tcatcactaa ttaagttaaa
     1501 cagaaacaga cagaattatt acatcctatc tccctttatt gtttttcttc ccagtcgtgg
     1561 taggcagcgt agagctgact tttattccac tggtaaatct tagcgacttc cttgatagct
     1621 tggtttttct tcacaccttg ctggatgcgg gtttgaattt ctacgaacaa gtcttcctcg
     1681 tccttttcct ccacaccctg actggcacct tcaacaataa gaaggcattc gcccttgagt
     1741 ggcgtttcag cgatgctttc cagcaattca gaaatggtac ctctttggta ttcttcatag
     1801 attttagtca attccctgac caagactacc gagcggtcac cgtagacttc tagcatattt
     1861 tccaatgtat ctgccacacg atggggcgat tcataaaaaa tctgagtttc aggatagtct
     1921 tttttcgagt cgaaaaattg cttttgctgg cctgattttc tcggtaaaaa gccgtaaaag
     1981 atatgtggct gcggcgctaa accactggca atcaaggcag aaattcctgc agaggcacct
     2041 ggaactgtca caactgcaat ttcttcctca atagctgcct taaccaaatc atgaccaggg
     2101 tctgaaatgc taggcaaacc ggcatcagag acctgagcaa tactttgccc tgctttcaag
     2161 aaaccaatca aatcaggaat tttttccttg gcattgtgct catgaaaact gatctgcttg
     2221 gtggaaatgt caaaatgctt gagcaaaaga cctgtattgc gcgtatcctc agcagcaatc
     2281 cagtccactt ctttcaagat ctggatagca cgaaaagtca tatcatctag attgccaatc
     2341 ggcgttgcca ctagatacag cttgccatag ggagactgcc ccttaaaact tttttgaatt
     2401 tgcatgccta ctccctgtat aacaactcat cacagaacat acattcttcg tcctgctctc
     2461 gacgttgtcc ataaaaatca ttacatacgt gaaatccatc acggtaaata cgacggacac
     2521 tttcgcgaac atgcttggcc ttgacgggag cgtctgcttc cacctcaccc aagcgttctc
     2581 gcaacttact attttccaag cgaagagctg tattttcctc taccaggctc ttgagatttt
     2641 tcttgatggc ttccacatcg gctaaggtta ccaataactg ttgggaaaaa tcatccagcg
     2701 cgtcaaataa ttcttttttg tccataaaac agccctttcc tttctttatc gttcattttt
     2761 gagtttatat ttctttcaag accagatatt ccatggtatt ttgaaaactg acattagcct
     2821 tccacatttt tctagcttct agtaaatctt gtagaatcac tcttactctt acctgcaaga
     2881 ggtcctgccc acagagaact tcaagaatcc gtaaaacctg atcctgtttt tccttatcat
     2941 ctgccaagtt ggctaatttg gcaacctgta gataactttc ttttttctta gctactaacc
     3001 aagtcagcag gcgttcactt tcatcgacca aggtccaaaa acttgcctga ttagccaact
     3061 tttctgcttc agctcgcgat tgactaaact tagctaaaag agtcgctttt ttcttaacaa
     3121 gtcccatttg ttctaagagt aagataagtt tttcttcttg ctttttaaag tggaagatct
     3181 gagtccgact tcggattgtc ggtaacatct tttcctcatc gctagtcaag aagaaaatat
     3241 aaacttcact ctggggttct tcgatgacct tgagcagaga attggctgcg ttgggatgca
     3301 ttttatccgc ttgctcgatg ataaagacct gttgctggct ttcaatccct gcttgagaaa
     3361 actgtcccac caattctcga atgcgttccg tcttaatgac ctgattaact ggtttaatca
     3421 aggtgacatc gggaaattct ccctgttcaa tcagcttgca acttcggcat ttctcacatg
     3481 gtaagacgcc aactttatcc gtacaaaaga ggctcttagc taaaaattgc gccatttcca
     3541 agcttccaaa gaaacctgaa aagagatagg cgtgattgag ctggtcttgt tctaagatac
     3601 ggacaaaacg gtcaaactga gctggttgcc aagcctttag ttgatcttgt ttcatttggc
     3661 caagtccatt ccgtcaaaca agacagcctt ggtagtttcc acaacttgct ccaaagggag
     3721 actagcatca atcttgacaa tgcgatttcc ctctttatcc agaagagaaa ggtagccttg
     3781 acgaactttt ttatgcaagt ccaacccttc caaatccaaa cgattaacct cgcggtcact
     3841 attagcagca atacgagcca gcccttcttc cacctcgatg tcaaaataga gtgtcaaatc
     3901 gggtttgagg ccatctgtcg caaactgatt gagccagtca atggcttcaa tatctaagcc
     3961 acgaccaaat ccctgatagg caacagaact atcgataaaa cgatccatga tgaccaactt
     4021 gccagcttca agggctggaa gaactttttc caccaaatgc tgtctgcgac tggcaatata
     4081 gagaagtagc tctgttttag catccatctg agtatgactt ggatccaaaa tcacttcccg
     4141 aatcttctcc ccaatcaaga ctccgccagg ttcacgggtc gtcagcacct ttactccttt
     4201 ttcctctaaa attggtagca gagcctctaa aacactggtc ttgcctgctc cctctggtcc
     4261 ctcaagagag actaaaaatc cttttgacat gtctaactca tttctttttt actacttcta
     4321 ttctatcaaa aaaatagggg tttgtgacaa tctttttgtc ttctcatttc atactcaatg
     4381 aaaatcaaag agcaaactag gaagctagcc gcaggctgta cttgagtacg gcaaggtgaa
     4441 gctgacgcag tttgaagaga ttttcgaaga gtatcaacca ccataaaaga ggcagataaa
     4501 actacctctt atttacctag ttcttgtgtt cgtttgtagg cttgatgaac tgcatccatg
     4561 actgttcctc ggaaagcatg cgcttctagg cttgctacgc cagcaatagt cgaaccgcct
     4621 ggactacaaa cttgatcttt caaaactcca ggatgttgct ggctttctaa gaccaattgc
     4681 ccagctccta ccacagtttg ggctgccatt ttcaaggctg tttctcgtgg taatcccgtc
     4741 tgaacacctg catctgccaa ggcctcaata aagagataga caaaggctgg tccacagcct
     4801 gcaagacctg tcgctgcatt gatcaagctt tctcctaatt caaccaagag accagccttg
     4861 gataaaagct gacaaaagag ctcactgtcc tcagccctgc aattaggaga caaggcataa
     4921 ctaatcactc cttgcccaat agaagcaggg gtattaggca tcatacgaat aattcggtgt
     4981 tggcttggga taagacttgc tagtttttct aaggtcaatc cagctgccat cgaaatcaaa
     5041 agaagacttt ctcttttttc aaggatggtc tggtattgag aaagcagttc agaaaactga
     5101 gcaggcttaa cacctagaaa aatcacatct gcttcttcga atatttcttc attgttagaa
     5161 gtctgaccac cgaagttagc gatgaaagca tccaccttga cttgactacg attggcaagg
     5221 agaatctcat ctgacggtct agcctgcaag acagacttgg ctaaactagc acccatattc
     5281 cccaaaccga taaatccaat cttcatctct tactccctta tctgtccatc accagtgacc
     5341 acatacttgt agctagtcaa ttccttcaag cccatgggac cacgcgcgtg caatttctga
     5401 gtagaaatcc ccatttcaca accaagacca aattgtcctc catctgtgaa acgagttgag
     5461 gcattaacat acaccgctgc agagtccact tgatctgtaa agtatgctgc agcttcagca
     5521 ttttccgtca caatagcatc cgaatgatgg gtactgtggg attcaatatg cgcaaccgct
     5581 tcttctaaac tgctcacaac cttaacagct agaatatagt ctaaaaactc ggtatcaaag
     5641 tcttgtgctt gagcagcttg acctgaaaca aactggcttg ctttgctatc taggcggaat
     5701 tgaattggtt ccaacccagc ttcttttcga tctgcaacca gcacttgctc caagcgagga
     5761 aggaagcttg ctgccttgtc ttcatgaacc agcagaacct ccatggcatt gcagacagaa
     5821 ggacgactgg ttttggcatt gttgatgata gacagtgcct tgtcgtcatc tgcgtcctta
     5881 tcgacataaa catggacaat cccagttcct gtctcgataa caggcacaat ggcattctca
     5941 actactgcat taatcaagcc agctcctcca cgaggaatga gaaggtctag atagcccttg
     6001 gccttcatca tagcataact actttcacgg ctagtatcct ccaccagttg aatcacattt
     6061 ggatgaatag tagtcgtctc caagcccttc ttcaaggctg tgacaatggc atgggttgtt
     6121 tgataggcat ccttaccact acgaagaaca accgcatttc cactcttaag agtcaaagca
     6181 gccgcatcag acgtcacatt tggacggctt tcatagataa taccgatgac ccccatagct
     6241 acacgttttt ttgtgataac caaaccattt tcaagctgac ttgtttctaa aacttcaccg
     6301 attggatctg gtaaggcaac cacttcacga attcctcttg ccatcgcttc tatacgatct
     6361 gcatccaaat aaagacgatc caacatcaca tctgagattt tccccttagc cgctgccata
     6421 tcgagggcat tagccgctaa aatttcctca gtagcagcca ctaagtgatc agccatggct
     6481 agcaagactt ggtttttcac ttcttcacta gctgtgttaa tcgatttttt aacagcctgt
     6541 acctgttcaa attgttcttg tctactcacc atagtttacc tctaaaattc tgtaaaaagt
     6601 agttggattt caggagtaat ggaaatccag tcgtcacggt aaatcaagac acccttggct
     6661 ttttgagaac gcaacatatc ctccaaagca gatgctccaa attgcacgcg tccttttcca
     6721 agtgattttc cactttcctt gtcaaatact gtcacgatat caccgtaaga aaagactcct
     6781 tctgcttcaa cgataccaga taagagaaga ctctttccat gttgagagag agcttccgca
     6841 gcccctttat caacccaaat agaaccttga ctctgagcat agaaggcaag ccattgtttc
     6901 tgggtacgaa gccccttctc ttgagcaaca aagtaagaac catcctcggt ctcctctgcc
     6961 gcctcaatca tggaatctga tttcaaggat gagcagatat aaacaggaac tcctgattcc
     7021 gtcgcgatag ttgcagcctt gattttggtt aacatacccc cagttccgtt tgacgaacca
     7081 gctccaccag ccatatcaat aatctcacga ttgatggtct cgattctctc caagcgtttg
     7141 gctcttggat ctgaattagg atttccagta tagagaccgt ccacatctgt caagagaacc
     7201 aaaaggtctg cttggaccat cgccgctact tgggcactta gagtgtcatt gtccccaacc
     7261 ttgacctcat caataacgac actatcattc tcattgatga taggaattgc cccacggttg
     7321 agcaaaaccg acaaagcctg atgggcattt ttataacgac gcttatccac aaagtcatct
     7381 tgggtcagca agatttgtgc agaaacgatt tgacgcaaga gaagattggt tgtatattct
     7441 tccaacaaaa gcccctgccc taccgctgct gaagcctgtt tatcagcaat tttagtcgga
     7501 cgctttttaa atcctaaggc tccaaaacca gccgcaatgg cacctgaaga caccaaaatc
     7561 aactcatgac cagcctcgtg cagcatagcc aactgctggg taatatcctt taccttacta
     7621 cgtgataaac ttccatcctc atttgtcaga gaagaagtac ccaccttaaa gacaatccgt
     7681 ttgtatttca tagtctcact ccgattattc atatttatcc attataacat gaataacagg
     7741 aaatagaaaa gagttgatag gaaaaagaag gccctaaaac cttcttttct actactgttc
     7801 tgattccgat ttgtttacac tcgtttcaga gctttgtgaa gttgaggatg tcaaatcttg
     7861 actagttgaa gaatttgtaa cactttcatg ttgactcgtt tctttatttt caagatcctt
     7921 tttcactact tctttggatt cttttatttc tgaatgactt gtagttttaa ttgtagcaga
     7981 tttctcgatc ggaacatcca ctaaccaggc accacttgaa tccacagtat agccttctgg
     8041 tgtcttacca ttcacaagta attgaccatt ttctttcaag aagtaccatt tatccttgtc
     8101 tttaatccaa cctatagctc gtgaaccatc tgtcttgtag aaataccaat catttgcctt
     8161 tttaagccag ccttgcttca tagctcctga ttcttctaga tagtaatgat gtccagcaac
     8221 ttctatctca cctgtcttca tgataccagt agaatccata tagtaccagg cttctttatc
     8281 ttttacccag cccgttttca tttcccctga tggagcaaag taataccatt gccccttata
     8341 ttgaagccaa cctgtcttca tagagccatc aacatccaaa taataccact gctctttatc
     8401 ttgaatccaa ccaatttcca tctggttttc tttgttaaaa taataccaga ttccagcgat
     8461 tttcttccaa tttttagcag caacaccaga gtctgtcaaa tagaaccaac gattgttcca
     8521 ttttttccat tgattctgta ataagatacc cgtttggtta aagtaatacc attcaccctt
     8581 aatttcattc caaccgacag catattctcc tgtagaatca ggcgcttgat accaccagtt
     8641 tccatatgaa ctcttatgcc aaccagcttg aaaacttgga atcggcttgt aggaagttga
     8701 aatgtttact aaaccgtctt gtcgaatatc aaaaactgtt gcatcatagt ctttgctggc
     8761 tgcgttgatt ctctcaattc ctcgttcttt gagccaatta acatactcac tatcaacacc
     8821 atttttccaa ggtagactat ccgaagtttg aacaatcaaa ctcggactca aatttttaat
     8881 gaaatccttg gcatttgatt tgttggtatc atggtgatga ttaaacttca tcaaatcaac
     8941 ttttccaatg agaggaccat acttgtcttc tgctccatga acattatcta aatcgccccc
     9001 aaggtaaatt ttcttgccat tgactttcac cacgctaatc aaggaattgg aattgtcatc
     9061 ccaaattttc tttaattcac ccgatgaatc agtttcattt tcataattat agagctgaat
     9121 attcatgtcc ccaaactgaa aatgagcatc cccttgtgtg atattttgaa taactgaaac
     9181 acctttttct gcagcagtct gtaaaacctt atcatagcca tacagattat cccatagacg
     9241 ttcagaatta gtaatacgac tatcactata tttcttaaga tagactcggt caactggata
     9301 ggtagacagt aattcatcaa catttccaat atgatcactg tgggtatggg tcaccaaaat
     9361 aaaatcaagt ttttggacac ccaattcctt caaacgacga aagacacggt ctgttagaac
     9421 atgcttataa gacgtttcaa ttccttctct ccatggatag cgagaatcac taccgtctgg
     9481 gaagtcataa tcttctcctg tatccaccat ggcaaaatgt ccattgcttt caagaataat
     9541 cgcatcactg ccaccttctt gaacattgat aaagtggatt ttatttcctg aactttcttg
     9601 agcctgaaca ttcccatacc atgacaaacc taaaaaagcg cctacaagtg ctaaactagt
     9661 taatttcttt ttcattctta ctttctcaat ctctccaagg tttccttaaa aatctctggg
     9721 atatctgctt taaattccaa ggtcttacct gttctcggat gagtaaaacc taaagtcttg
     9781 gcatgaagaa attgtccatg tcctttcaaa gtcttgcgag gaccatagac ctcatcacca
     9841 gcgactggat ggccgatata agccatgtgg acacggattt gatgagtgcg ccctgtctcc
     9901 agttgcaact ctactaagct ataatcgcca aagcgttcca agacgtgaaa acgcgtcact
     9961 gcaggcttcc ctttagcagt tacagcctgt ttcttacggt ctttttcact ccggccaatc
    10021 ggcgcttcaa ttacaccacg atcattaggc agatttccat gaacaatcgc ccaatatttg
    10081 cggagagact ttttatcctt gagttcttgg gcaagtgcta gatgcgcatc atcgttttta
    10141 gcaatcatga gaagacctga cgtatcctta tcaatacggt gaacaattcc tggacgcaga
    10201 accccattga tacccgacaa gtccttaata tgatacatga gggcatttac tagggttcca
    10261 ctggtatgac cagcactcgg gtgcacaacc attccctgag gtttgttaac gacagccaca
    10321 tcctcatctt ggtagactat ttctagcgga agatcctcag ccacatactc taatacctct
    10381 ggttctggca catggtaagt gacgacatca ccctcttgga ctgtgtattt agctttcttg
    10441 acttgaccat tgaccaagac ctggcctgat ttaatttgtt cattcgcaag actgcgtgat
    10501 aattctgtca aatctgacaa agccttatcc aaacgcagac caccagtttc aattttaatt
    10561 tccatttatt tcctctttta gcattgcaat caataaaata atcactccaa ccgtcagata
    10621 gctatctgcc acattgaaaa ttgcaaagtt gataaagtca aggtggaaca tatccacaac
    10681 aaagccctga ctggccctgt caataaagtt tccaagacca cccgcgatta tcaaagtcaa
    10741 acccaagacc atccagaatg agtcctccat gtgtttatgt aaataccaaa tggcacctat
    10801 cacgacaacc agagtaatga cagcgaataa cagctgctga tcttgtaaga tagaaaaggc
    10861 tgcacctcga ttttgcaggt aggtcaagct aacgaaattg ggaatccagg agcgcacttc
    10921 acccagtgga atctgctgga cgatatagga tttgaccaac tgatccagcc caattaaaag
    10981 cagtacaatg actgccacta ttgctctttt tttcatgatt tcctcttttg atcaaaatat
    11041 tcttgcatga cttctacgaa gagagtccca gcttgactaa gctccacttc ttcacgttta
    11101 acatagacca tgcggttatc taggttatcc ttgagacgaa taactgtaat gccattaaca
    11161 ctgtcactat ctaaaaatcc agaacctgtc gcataggcgt ccgtccgctc caaaatacca
    11221 ttcaaggtgg cacggtctgt cacattaaac atctgtgagc tagcgctggt atcgacaaag
    11281 ttctctgaat aataaaggta ctcgtctttc tcttgagtga aacgaaccgt tggtaaatcc
    11341 gctaaatcct ccatgactaa ttcctctttc tgagctaaag gatgaccctc acagagataa
    11401 atatgggtat ggaaaggaat caattcgatg acctccagac ctaacttttc aacccgttgc
    11461 ataatcccct ttttattttg attgttgagg tagataatcc caatctcact atgcccttgc
    11521 gccacttcat ctaatatttg aacagtagtt gattcaaaaa tacggaagtt cttatagtca
    11581 ggatagcgct ctgaaaaggc cgtaatagtt ggtggtaaga agtcatagtg ctggctagca
    11641 acggaaaatt catctttttc ttcttcagga ttggcatact gattttgaaa aatatcaaat
    11701 cctttaacca attcttgcgc tttttcataa aattccatcc cacgacgggt caagaaagtc
    11761 cctgagctgg tccgacggaa aatcttaaag cccaactctt tttccaaatc acgaacagaa
    11821 atagacagac tcggctgact aacatacatc ttttcagcag cttcacgaaa agtaccacta
    11881 ttggcaatag ccacaacata gcgtaattgt tgaatgttca tcttctaccc ccaacttctt
    11941 tatcttttca ttataccata ttttagaagt tttccaaaaa ggaaaaaaga acatcctatt
    12001 cttcttaact atcttcacta tctgcctttc tcacgccaat cttattttca aaatcaattc
    12061 aaaaaaataa gtggctacac accacttcag tatcggaaag aaaaacgttg actatttgtg
    12121 aaaaaagaaa atgccggaaa gttccgacat ttttttagta agctaacttc ctgaaaatag
    12181 gagtaaccaa acatcccaga agacagtgat attgatgaga atatgaacta gtattggata
    12241 gtagagattt ttagtcatgc gatacaatag agctaaaata agacctccac tagcataaat
    12301 gaaaaaagca agaggagtta aagcaaaatt gatatgaata taaccgaaaa taatagcaga
    12361 aagcaaaaca tctccgtacc aaggtgagtt tttgaaaaag gttgtcataa gcacacctcg
    12421 ataaatcaat tcctcagcaa taggggcgat gaagcaaacg atgagcaaga aataagggaa
    12481 ctcctgtctc cccatcattt ctaccgtttc attcaaagaa atctgatttg aagacaggga
    12541 tatgaaatac gaaaagagga agtcagacat atatgaaatg atgtagccca gtaaaaggta
    12601 gatgaagtac ctcagctgcc acttgaaatg aaaaattttc tttcctgcaa aaaccagtag
    12661 atagatgacc gctaagaaaa gaactccgct ctccatcaaa agcagaatct gaaaaaattc
    12721 acgacttgct ggtagatagg gctttgcgag gtgattgaaa actctccaaa accaagttga
    12781 tttgtaaaaa attaaggaca atgctagtaa aatttgaata gcccgttttt tcatattaaa
    12841 ttctctgctt tctccacttg tttcttcata ttactaataa attttactta aaatctcgca
    12901 gcacttcctt tgcaaagatg attgcctcct ccaatatatc ctaaatcata ggtgcctctg
    12961 ggacaaaatc catgaaccgg cagagttaat actggttcta tttcccaatc gtacctacca
    13021 ttaggatttt taccatatga atctgctcca ccttgtatca gctctaattc ttcatttgtt
    13081 agatttatat tttttgtcat taaaatcatg atatatttcc tccattattc ttcagattag
    13141 ttgagtaatc tgatactcat acctacttac aaaaaaacta ttatattaag ttggtttttt
    13201 aatttgtcta gttgcaacat tgacaaacat agtatagcat atctttgcga aatatcctct
    13261 tcaaatcatg aattgtcatc aaaacatctt aaactataaa atcaattagt ctcaagcttt
    13321 ctatcaattt cttctccaaa atatgctata ataatagcaa aagataaaga aggaagacct
    13381 atgattaaac tactagcctt ggatatggac ggcaccctcc tcaatgaagc caaggaaatc
    13441 ccacaagctc acattactgc tattcaccaa gccattgaaa aaggtgtcaa actggttctc
    13501 tgtacgggtc gccccctttt cggtgtcctc ccctactaca aaaaactgga gcttgacctc
    13561 cagaacgaat atattattgt taacaacggt tgttcaactc accagaccag tgattggagt
    13621 ctagttgact ggaaagaact gagtccagct gacatcgaat acctctatga ccttgctgaa
    13681 aagagtgatg ttcagttgac actttttgac gagtcacatt attttgttct cggtggcaaa
    13741 cccaatcaag ttattgaaaa tgatgctaaa ctagtatttt cagacctgac tgaaatttct
    13801 cttgaagaag cgactagtgg aaagttacgg atgttccaag gtatgttttt aggaacaaaa
    13861 gaacaaacag acgattttga gcagcgtttt gctgaagagc tttgccaacg attcagtgga
    13921 gttcgttcgc agcctgtcat ttatgaagca atgccgcttg gaacgacaaa ggctactgct
    13981 ctttcacgac tagctgagat tttgaagatt gattcctcag agattatggc catgggcgat
    14041 gctaataacg atatcgaaat gctccagttt gcagggcttg ggattgcaat gggaaatgcc
    14101 agcgattatg tcaaatctct tgcggatgcc gttacctcaa gcaacgaaga agacggcgtt
    14161 gcgcgtgcta ttgagaaata tattctataa aaaagaaaag caaatagaca gaagttactg
    14221 ctatttgctt ttttgctaat attttaaaat acggactaat caatagacaa gaatagcgaa
    14281 tcataaaaat cgatttacta gatgccatag tatcttatag ttgctaataa gaagttagtc
    14341 tagcagaatg aatctctcat ttctcccaca ctaatctgta atttgtctat acatttctgg
    14401 tcttctatct cgaaacaagc cccagtttag gcgttcactt gctcccttgt ctaggtcata
    14461 agtagctaac agaacagctt cttcttgtct ttcagctcgt tctagaatag ctcctgtttc
    14521 atccgtcata aaggaggaac cgtagaagtc aagactggaa ctctgtccgc cattttcctc
    14581 actaggagta acctcctcta aaccataacg attggctgcg atgactggaa caatattcgc
    14641 tgctgcgtgc ccttgcatag tacgttgcca gtgaccacaa ctatctgtat ccaaaatcgg
    14701 ctctgaaccg atggctgttg gataaaagag caattctgcc ccattcagag caagacagcg
    14761 ggctgtttcg gggaaccatt gatcccaaca gataccgata ccaatcttag catagcgagt
    14821 attccagacc ttgaaaccag tgttaccagg cgtgaaatag aatttttctt gataataatg
    14881 gtcatctggt atgtgggtct ttcgataaac gcccagcact tccccatctg catcaatgac
    14941 ggcaatagag ttatacaaga cattaccatc tttttcatag aaactgattg gtaaaacaac
    15001 ttgtagttcc ttagcaatca ccttaaaatg ctgaatggca gtattttccg ctacagattg
    15061 ggcatactgg tagtagtcat actgacgttc ctgacagaaa tagggatgtt caaacaactc
    15121 gggcaagaga ataatttggg ctccttgctc agcagcctga cgtactaaac gctctgcggt
    15181 ttggatattt gttgccacat ccttagcgca ttgcatctga atggttgcaa ctcttacatt
    15241 tctcatcttt ttctcctatt ctggaatttg ttgggtgata cagtggatat tgccaccacc
    15301 taagagaata tctctggctg gtattccgac aactttacgg tctgggaaac acttgctgag
    15361 gatatctaag gccacttggt cgtttacatc ctcaaactgt ggaaccaaga cagccttgtt
    15421 ggcgatataa aagtttacgt aggaagctgc tagtcgttca cctgcgtatc gcttttcttc
    15481 tccttcttca tagatgtagc ctggcaaatc ttcttctgtc acaacttgtc gaactgcagg
    15541 gataggcaat ttatgaatgg tgaagtgaca accttttgca tctgtttcct gttctaagag
    15601 ttcgagatct gcttttgaca tggcatactg gggatcattt tcgtcatctg tccaagctaa
    15661 aacaacctca gcaggaccaa caaaggcagc aacattatcg acgtgttcat tggtttcatc
    15721 ctgataaata ccataaggaa gccaaataac tttttcagca ccaagacttt ctaataatgt
    15781 gttttcaatc tcctctttag tcaggttagg attgcgacca ggactaagca agcaactttc
    15841 agttacgaga atagttcctt gaccatcgct atggattgcg cctccttcca gtacaaaagg
    15901 tttagcatca tagacaggct tttccaagac ctcagcaaaa cgactggcta cttggtcatc
    15961 ctcttcataa tcttgataaa gaccatcata ggtgcctccc caagcattga aggcccaatc
    16021 cacggctaat ttcttacctt tatcattgac gagaatggtt gggccagtat cacgcgccca
    16081 ggcatcattg gtgggaatgt ctaaataaac aaccttgtct ccaagatagg attgggcttc
    16141 agatagatag gcctgctcca ccaaaagata gactctttcc ccttctgcta tggtctcgat
    16201 gatctgagta aatgctcttt tagcagcctt tccttgaaaa ggccatgatc ctggtcgagt
    16261 cggccatatc atgagggtac catgatgggg ttcgtactct gctggcatgt gatagcctaa
    16321 tttttttgga ctgtccatca tgataatctc cctttaaagt cttgatagcc aaaagctttg
    16381 agtaagctac agtctccctg ttcgtccatg agatagagac ttggcaatcc aataccatta
    16441 aaggtattat ttttgacaaa agaataaatg gccatgtctt gaaaataaag tctgtctccg
    16501 atttggactg gattttcaaa actataatca ccaatcacat cgcccgtcag acaggtatta
    16561 gaagaaagtc tgtaggtatg ggctttttcc tgtgactcaa agccatttct caaaggtgga
    16621 cgatagggca tctcaagtac atcaggcata tggcaggtcg cagaggcgtc taaaaccaag
    16681 atttccatac cgttttctac aatatctaat acctcagttg ctaaataacc cgcattaagc
    16741 gcaatggctt caccaggctc gatatagatt tcaagattgt aagtttttcg gatacgcttg
    16801 atttctgaaa tcagcaaatc cacatcgtaa ccttctcttg taatatgatg accaccaccc
    16861 atattgagcc attttacctc atgtaagtag ggaccaaact gttcttctac tgctttcaaa
    16921 gttgtttgta aatcatctgc tccctgctcg caaagggtat gaaaatgaag tccgtcaacc
    16981 aaatctagca aatcactcgg aatcttgtct atagtaactc caaagcgaga accaggtgca
    17041 caagggtcat agagcgcgtg atctccttga gttgaacact gagggttgag gcgcaaacca
    17101 acactgacac cagcctctcg acaacgagcc ccatgcttac gcaactgtct ctctgagtta
    17161 aagacgatat ggtctgttat cccaagcaat tcctccaagt ctgcatcctt gaaagcaggc
    17221 gcaaatacat ggacttcacc aggaaattct tcccttgcca atttggcctc atagagtcca
    17281 ctagctgtcg tacctgatag atactggcta atcaagggat aagttttgta gagggaatat
    17341 gccttctggg caagcaagac cttgcaaccg gcctcttctt gtacatattg tagaatgcgg
    17401 caattagctt ctaacttggc caagtcaata acataggctg gtgttggtac ttgttctaac
    17461 ttcattagtc caccatttgt ggattttcaa ccacaaccca tggcaaacca tactcattca
    17521 aagcttccat gaatggatct ggatctaact cctcaaggtt atacactcca gcttgtttcc
    17581 aagttccgtt catgactaat tttgtcccaa tcatggctgg aacgcctgtc gtgtatgaaa
    17641 ttgcttgtga cccgacttct gcatagcact cttgatggtc gcaaacattg tagatgtaga
    17701 tagtcttttc aacgccatct ttgacacctg taaagataca tccaatattg gtttttccga
    17761 ctgtacgtgg cccaagactg gcaggatctg gaagcaaggc tttcaaaaat tgaattggaa
    17821 caatttcttg gccgttaaag ttaatggtat ccgtacgaag gagtccaaca ttttcaagac
    17881 atttcatgtg cgtcaagtaa gattgaccaa aagtcataaa gaaacgaatg cgtttgacac
    17941 ctggaatgtt cttggccaat gattcgattt cttcatggtg aaggagatac atatcttttt
    18001 gtccaacttg agggaaatca tactcacgct tgatagacat agcttcgact tcgacccatt
    18061 tcccatcttc ccagtaagaa cctggagcag aaacctcacg gagattaatt tctggattaa
    18121 agttggttgc aaatggataa ccgtggtcac cgccattaca gtctaaaatg tcgatataat
    18181 ggatttcatc aaaatagtgt ttgagggcat aagctgaaaa gacactagtt acacctgggt
    18241 caaaaccaga accaagaaga gcagtcaagc ctgcttcttt gaatttctct tgataagccc
    18301 actgccatga gtagtcaaag taggctgtaa aaccaagttc cttacaacgt ttttcgtaga
    18361 tagcacgcca ctcagggtct tctgtgtctt ctgcttcgta gttggctgta tcgatatagt
    18421 gaacacctgt tgccaaacaa gcatccataa tggttaaatc ttgataaggc agagctacat
    18481 tcaaaacagc ttctggtttg tagctttcaa tcagggcaat cacttcttca accttgtcag
    18541 catcaagtgc tgcagtttca attttagtac ttgttttgcc ttctagcttc gctttcaagt
    18601 catcgcattt tgacttggta cggctagcaa tcataatctc tgtaaatgtt tcgctatctt
    18661 gacaaatctt tgaaatagca acttgggcaa cgcccccaca accaataact agtaaacgac
    18721 tcattttttt ccttcctctt cttctaaaat gtcctcaaca tacttgggca acataaagga
    18781 tcccacgtgt aagtttgcag tgtagtattc tgtgaaaagc tggcgttttt tccaaccttc
    18841 cttgtcaaaa tctttgacag ggtggtattt tttcgatgca aatccaaaca accaatagcc
    18901 agctgggcta gttggaatat gggcctgata aacccgactg attggaaagg attgattgac
    18961 cttgcggtgc atgcttcggc aggccgactc atcctcgtca aagaagggac tcccatgctg
    19021 gtaaatcatg atgccgtctt ccttcagagc tcgataacta ttgccgtaga attccttggt
    19081 aaagagtcct tccgtatggc caaatggatc tgtcgcatcg ttgatgataa tatcgtaatc
    19141 atcttcgcag tttcgcaaaa agcgtagccc attttggtag taaatggtaa cacgaggatc
    19201 atctagccct acagcaaagt ctgggaaata ctcacgacag acctcgacca acatctcatc
    19261 cggttccacg atatcgattt gttccagttc aggatagagt gttaatactt gggcaacacc
    19321 gccgtcacca cccccaataa ccaatacttt ctttggattt gggtggacag ccatgggaac
    19381 gtgaacggtc atttcattgt agacgaaatc atccgcatct gagaacaaga catggccatt
    19441 taaaatcagt attttcccaa aagctggcgt atccaagact tcgatatcct gccattcact
    19501 ttttccagcg taaagttgct tggctgttct cagagacaat ttgacatctg gagtatgaac
    19561 ttcagaaaac cataaatcca tctatttctc cttatattat atttagcgca gttagctact
    19621 gccaaagccc aagtggtata cttggaataa gccactgtgg attagtttat tccctccaat
    19681 cgcttgacga tttccgaaag gctctaacca cagttttttg tttcaattgg taatgagttt
    19741 gataacgcat agacgtttta tctcgtctaa ggtaacaagg caaaaaaatt cgtggataac
    19801 caagtagact tgaaatacta catctggagg tactcccatg ctttatgtgg gcattgatgt
    19861 cgctaaaaat aaacacgatg ttacagcctt gaatgttcca ggaaaaactg ttcttaaacc
    19921 actcactttt tcaaataata aagctggttt tgaactctta gatctgtctc ttcgacagct
    19981 caaccaagac tgtctcatcg ctcttaaact tctttctgat cccaatcgtg aacaatttca
    20041 acacgataat cggcaagtag acctaaaaat actggctaga catattcatc gtctcaagaa
    20101 aaaacagtct gattggaaag tacaatacac tcgttgtctt gatatcatct ttcctgagtt
    20161 ggataaaatc gttggaaagc attcagaata tacctaccaa ctcttgacac gctaccctaa
    20221 tactcagaaa aggattgagg caggatttga taagctgata gaaattaagc gattgaccgc
    20281 ttctaattca agggcttttt gccgtgctct aaaatcatcg ccaaatcatc aaattgaaat
    20341 aagaaatagt ttagataaaa ctattgactt ttcttatagt ttgtctttct cttaataacg
    20401 ttgatatgat tgacctctgg atcttccgtc ccttggaggg agcaaccacg ttccttggcg
    20461 aattggatat agtcgacaat ttctcgtgta atgcgttcac caggagccaa gataggaatc
    20521 cctggaggat agcacatgac aaattctcca cagacctgtc caacagactc atctaaggtc
    20581 aaactttttc tctctgaata gaaggcttct tgtggagaca gcaccaactc gggctgaata
    20641 tattctccag ctattaagtc cttcccatct cttgaataga gtctcttaat atcagccaga
    20701 gcaccaacca agcgctcgat gtcttggatg cggtcgccga tggaaatata ggccaagata
    20761 ttgccgatat caccaaactc aatctgaatg tcgtattcgt ctcgcaagag gtcataaacc
    20821 tcgatacctg ttaagcctat tccctgagtg taaactgaca acttggttac atcaaaatca
    20881 cagaccgaca caccatctat taactctttt gagtaagcat agtaaccgcc aatggcattg
    20941 atttcatgac gagcgtactc ggatagctca atgacttcct caaacgactc tttaccacga
    21001 agggccaagt tgcgacgtga aatatccaaa ctagccatca ataagtaaga ggcagatgta
    21061 gactgggtca ggtttataat ctgacgaacg tactcgggat tcatctgctc cccgattaaa
    21121 agaagcgaac tttgtgtcaa actcccacca gacttatgca tggaaactgc tgccatatca
    21181 gctcctgcat ccatagcaga aattggaagt ttaccagtga aatgcaaatg cgctccatgg
    21241 gcttcatcta ctaaaaccat catgccagct tcatgagcca tttctgtcaa cccctttagg
    21301 tctgaacaga tgccgtagta agtaggattg ttgattagga tagccttagc atctggatgg
    21361 tcctttatgg cctgtgctac tcggtcattt tcaagaccta aagcgatacc aatcttagga
    21421 tctacactca tctcgatata gatgggaatg gcaccacata gaaccaacgc attgatagca
    21481 gatttatgga catttcgtgg cagaataatc ttatctcctg ccttgcaggt tgccagaatc
    21541 atagtctgca ccgatgaagt tgttccacca atcattagaa aggcatggct agctccaaaa
    21601 gcatctgcag ccagctcctc tgcatcacga ataatcgaaa taggatggcc taaattatcc
    21661 aagggtttca tcgaattgac atcaatgcct acacattttt ctcctaagag ttcgacaagt
    21721 tctggatttc cccgtccacg cttgtgacct ggaacatcaa agggaacaat ccttttcttg
    21781 cgtaacttca ccaaggcctc ataaattggg gcttggtttt gatctaactc tttcaaaaca
    21841 tactcctttc aatattttta ggaccttatg aaaactaagg aactaaaggt tgactcatga
    21901 aaaaagagca ggttttcacc tgctctgcaa tcttctgacg tgtttatttt ggtttctgtt
    21961 gagtatgtct gttcccgatg tttcctaact ctctagtagc aaatattacg tactttattg
    22021 atcgtttcac aagatgacgt gtgctttcac cacactaaaa atttatgaat tagaacaagt
    22081 gtcctaacat caacttataa aagtaggctt ttaaccctat caataatgtg gcttttcaac
    22141 cacgcttcgg cattcaaccc tgcctgtccg taggcatttt ttactcgggt ttatatttgc
    22201 tagaaaacat catctcattt tctaagcttt attactatat catgttttta agaacttgta
    22261 aagtattttt tgataaaaaa
//
LOCUS       DAVKJD010000025        22068 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKJD010000025 DAVKJD010000000
VERSION     DAVKJD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22068
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.025"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(257..344)
                     /locus_tag="VW157_001014"
     tRNA            complement(257..344)
                     /locus_tag="VW157_001014"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(308..310),aa:Ser,seq:gga)
     gene            507..1247
                     /locus_tag="VW157_001015"
     CDS             507..1247
                     /locus_tag="VW157_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9552332.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1244..2155
                     /gene="pfkB"
                     /locus_tag="VW157_001016"
     CDS             1244..2155
                     /gene="pfkB"
                     /locus_tag="VW157_001016"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9552333.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSDGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2152..4104
                     /locus_tag="VW157_001017"
     CDS             2152..4104
                     /locus_tag="VW157_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9552334.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGSAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4268..6493
                     /locus_tag="VW157_001018"
     CDS             4268..6493
                     /locus_tag="VW157_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9552335.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDQDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(6644..7072)
                     /locus_tag="VW157_001019"
     CDS             complement(6644..7072)
                     /locus_tag="VW157_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552336.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            7210..8352
                     /locus_tag="VW157_001020"
     CDS             7210..8352
                     /locus_tag="VW157_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9552337.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8361..9575
                     /gene="thiI"
                     /locus_tag="VW157_001021"
     CDS             8361..9575
                     /gene="thiI"
                     /locus_tag="VW157_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9552338.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            9710..10534
                     /locus_tag="VW157_001022"
     CDS             9710..10534
                     /locus_tag="VW157_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9552339.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            10547..11713
                     /locus_tag="VW157_001023"
     CDS             10547..11713
                     /locus_tag="VW157_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9552340.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASEFEPQYCLATSRRANAHYVY
                     SEENLLAKYSQQFKVKKIMPEAFAELQGDYFYMLTTPSRQEMDQMIADFGQRQE"
     gene            12221..13714
                     /locus_tag="VW157_001024"
     CDS             12221..13714
                     /locus_tag="VW157_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9552341.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            13727..14269
                     /locus_tag="VW157_001025"
     CDS             13727..14269
                     /locus_tag="VW157_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9552342.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDELNLLVKSRYSEEVVA"
     gene            14266..15315
                     /locus_tag="VW157_001026"
     CDS             14266..15315
                     /locus_tag="VW157_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9552343.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIYIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIIVPYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSRFNEMFGDPLNNNKKFAVKTGQQCFKFSSGKFLDKHDRVFEGYPAYG
                     GNGIAWKSRKYLIDNPTIIIGRVGAYCGNVRTTHGKVWISDNAIYIKEFKNSDFNLVF
                     LLELMKVIDFSKFADFSGQPKITQKPLENQKYILPPLALQNEFADFVALVDKSQFACE
                     IAIKVWRNSLKFSII"
     gene            15345..18709
                     /locus_tag="VW157_001027"
                     /pseudo
     CDS             15345..18709
                     /locus_tag="VW157_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(19215..19685)
                     /locus_tag="VW157_001028"
     CDS             complement(19215..19685)
                     /locus_tag="VW157_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9552344.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(19702..21975)
                     /locus_tag="VW157_001029"
     CDS             complement(19702..21975)
                     /locus_tag="VW157_001029"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9552345.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHINKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         6737 a         3932 c         4834 g         6565 t
ORIGIN      
        1 aaaagaaaat cttttatggt ttagttaaaa agcaagagtt taagaaactg gattaattaa
       61 acttgaagta tttgagattt tgtcttttac tgttcgtaaa atcgtatgag ataagaaggg
      121 agtggaaaga attcatactc tctctagaac ttgtatattg aaacgatgtg gagattgtaa
      181 agaaattaca aaatacccca aaactaagat agaaatacaa agtttcaggg tatcatatat
      241 tttatctgtt tttcgacgga agacatggga ttcgaaccca cgcacgctat tacacgccta
      301 ccgcgtttcc aacacggcct cttaagcctc ttgagtaatc ttccaatact tactcaaata
      361 gtctaccata aaggctctta tcttgcaata aaaattctag aaataagaaa aaatgataga
      421 ttttgaaaga aaatgataaa aaatgcttga cttcgaaaga aagtatgata gaatgaatag
      481 tgtaaacgat aacaggaggt gattcagtgt taaaaacaga acgtaaacaa ctaattttag
      541 aggagttaaa tcaacatcat gtagtttctc tagaaaaatt agttagtttg ctagaaacgt
      601 cagaatcaac ggttcgaaga gacttggatg agttggaagc ggaaaacaag cttcgtcgtg
      661 tgcatggtgg agcagaactc ccccactcct tacaggaaga agaaaccatt caagaaaaat
      721 ctgtcaaaaa ccttcaagaa aagaagttgc tggctcagaa agcagcctct ctcattaaag
      781 aaaaagatgt catctttatc gatgctggaa caacaactgc ttttttgatt catgaattgg
      841 tcaataagaa tgttacagtt gtgaccaact ccattcacca tgccgctcag ttggttgaaa
      901 agcagattcc aactgtcatg gttggaggaa acgtcaagac ggcgacagat gctagtatcg
      961 ggggcgttgc tcttaaccag attaaccaat tgcactttga ccgtgccttt atcggaatga
     1021 atggtgttga cgatggctat tatacgactc ctgatatgga ggagggagct gtgaaaagag
     1081 ctattttgga gaatgccaag cagacctacg tcttggtgga ttcgtcaaaa attggacaaa
     1141 cttgctttgc caaggtagca ccactcaaac gcgctatcgt tatcactagt caagggcatg
     1201 agctcttgca ggttattaag gagaaaacgg aggtaataga agtatgattt atacagtcac
     1261 actcaatcca tccattgact atatcgttcg tttggaccaa gtcaaagttg gtagtgtaaa
     1321 tcgtatggac agtgatgata agtttgctgg tgggaaagga atcaatgtca gccgtgtctt
     1381 gaaacgtttg aatataccaa atacagcgac gggatttatc ggtggcttta ctggtaaatt
     1441 tatcacagat actttagcag aggaagaaat cgagacacgt tttgtccagg tggcagaaga
     1501 tactcgtatc aatgttaaaa tcaaagcaga ccaagaaaca gaaatcaacg gaacgggtcc
     1561 aactgttgaa tcggttcagc tagaagaatt gaaagctatt ttatctagtc tgacagcaga
     1621 agatacagtt gtctttgcag gttcaagtgc taaaaatcta ggcaatgtta tctataagga
     1681 tttgatttcc ttgacgcgcc agactggtgc gcaagtggtc tgtgactttg aaggacagac
     1741 cttaattgat agtttggact accagcctct tcttgtaaaa ccaaacaatc atgaacttgg
     1801 agcgattttt ggggttaaac tcgaaagttt agatgaaatt gagaaatacg ctcgtgagtt
     1861 actggctaag ggtgctcaaa atgttattat ctctatggct ggtgatggtg cccttcttgt
     1921 cacatctgat ggagcttact tcgctaaacc aatcaaggga acagtcaaaa attcagttgg
     1981 agctggtgat tctatggttg ctggattcac aggtgaattt gtcaaatcaa aagacgcagt
     2041 agaagccttc aaatggggag tggcttgcgg aacggcaact accttctcag atgacttggc
     2101 aacggcggaa tttattaaag aaacatatgg aaaagttgag gtagaaaaac gatgaaaatt
     2161 caagacctat tgagaaaaga tgtcatgttg ctagatttgc aggcaactga aaaaacagct
     2221 gtcatcgacg agatgattaa aaatttgaca gaccacggtt atgtaacaga ttttgaaaca
     2281 tttaaagaag gaattttggc gcgtgaagct ttgacttcta ctggtttggg tgatggaatc
     2341 gcaatgcctc acagcaaaaa cgctgctgtc aaagaagcga cagttctatt tgctaagtca
     2401 aataagggtg ttgactacga gagcttggat ggacaagcaa ctgacctctt cttcatgatt
     2461 gcagctccag aaggtgccaa tgatactcac ttggcagcct tggcagaatt gtctcaatac
     2521 ttgatgaaag acggttttgc agacaaactt cgtcaagcaa catctgcaga ccaagttatc
     2581 gaactttttg accaagcttc agaaaaaact gaggaacttg ttcaagcacc tgctaatgac
     2641 tctggtgact ttatcgtagc tgttacagct tgtacaacag gtattgccca cacttacatg
     2701 gcccaagaag cccttcaaaa agtagctgct gaaatggggg ttggtatcaa ggtcgaaacc
     2761 aacggtgcta gcggtgttgg aaatcaacta actgcagaag atatccgtaa ggctaaagct
     2821 attatcattg cagcagacaa ggccgttgaa atggatcgat ttgatggaaa accattgatc
     2881 aatcgtccag ttgctgacgg tatccgtaag acagaagagc taattaactt ggctctttca
     2941 ggagatactg aagtctaccg tgccgctaat ggtgccaaag ctgcaacagc ctctaacgaa
     3001 aaacaaagcc ttggtggtgc cttgtacaaa cacttgatga gtggtgtatc tcaaatgtta
     3061 ccattcgtta tcggtggtgg tatcatgatt gcccttgcct tcttgattga cggtgctttg
     3121 ggtgttccaa atgaaaacct tggcaatctt ggttcttacc atgagttagc ttctatgttc
     3181 atgaaaattg gtggatctgc ctttggtttg atgcttccag tctttgcggg ttatgttgcc
     3241 tactctattg ctgaaaaacc gggtttggta gcaggtttcg tggctggtgc tattgccaaa
     3301 gaaggttttg cctttggtaa aattccttat gccgcaggtg gtgaagcaac ttcaactctt
     3361 gcaggtgtct catctggttt cctaggtgcc cttgttggtg gatttatcgc aggtgccttg
     3421 gttcttgcca tcaagaaata cgttaaagtt cctcgttcac tcgaaggtgc taaatcaatc
     3481 cttctattgc cacttcttgg aacaatcttg acaggatttg ttatgctagc tgtgaatatc
     3541 ccaatggctg caatcaacac tgctatgaat gacttcctag gcggtcttgg aggaggttca
     3601 gctgtccttc ttggtatcgt ccttggtgga atgatggctg ttgacatggg tggaccagtt
     3661 aataaagcag cttatgtctt tggtacaggt acgcttgcag caactgtttc ttcaggtggt
     3721 tctgtagcca tggcagcagt tatggctgga ggaatggtgc caccacttgc aatctttgtc
     3781 gcaactcttc ttttcaaaga taaatttact aaggaagaac gtaactctgg tttgacaaac
     3841 atcatcatgg gcttgtcatt tatcactgag ggagcgattc catttggtgc cgctgaccca
     3901 gctcgtgcga ttccaagctt catccttggt tcagcagtag caggtggact cgttggtctt
     3961 actggtatca aactcatggc gccacacgga ggaatcttcg ttatcgccct tacttcaaat
     4021 gctctccttt acctcgtttc tgtcttggta ggagcaatcg taagtggtgt ggtttatggt
     4081 tacctacgca aaccacaagc ataaaaaata gaaaaatgaa aagattggac cgtttggtgc
     4141 agtctttttc tcttcccgaa atgcctgtga aatatggtat aatagaagaa tggcaaacaa
     4201 gaatacaagt acaacaagac ggagaccgtc taaagcagaa ctggaaagaa aagaagcgat
     4261 tcaacgaatg ttgatttcat taggaattgc gattttattg attttcgcag ccttcaaatt
     4321 aggggctgca ggtataaccc tttataattt aattcgcttg ctagtgggta gcctagctta
     4381 tctggcgata ttcggcctat taatctatct cttctttttc aagtggatac gaaaacagga
     4441 aggactctta tctggctttt tcaccatatt tgctggctta ctcttgattt ttgaagccta
     4501 cttggtttgg aaatatggtt tggacaagtc cgttctaaaa gggaccatgg ctcaggttgt
     4561 gacagatctg actggttttc gaacgactag ctttgctgga gggggcttga taggggtcgc
     4621 tctttatatt ccaacagcct ttctcttttc aaatatcgga acttacttta ttggttctat
     4681 cttgatttta gtgggttctc tcctagtcag cccttggtct gtttacgata ttgctgaatt
     4741 tttcagtaga ggctttgcca aatggtggga agggcacgag cgtcgaaaag aggaacgctt
     4801 tgtcaaacaa gaagaaaaag ctcgccaaaa ggctgagaaa gaggctagat tagaacaaga
     4861 agagactgaa aaagccttac tcgatttgcc tcctgttgat atggaaacgg gtgaaattct
     4921 gacagaggaa gctgttcaaa atcttccacc tattccagaa gaaaagtggg tggaaccaga
     4981 aatcatcctg cctcaagctg aacttaaatt ccctgaacag gaagatgact cagatgacca
     5041 agatgttcag gtcgattttt cagccaaaga agcccttgaa tacaaacttc caagcttaca
     5101 actctttgca ccagataaac caaaagatca gtctaaagag aagaaaattg tcagagaaaa
     5161 tatcaaaatc ttagaagcaa cctttgctag ctttggtatt aaggtaacag ttgaacgggc
     5221 cgaaattggg ccatcagtga ccaagtatga agtcaagccg gctgttggtg taagggtcaa
     5281 ccgcatttcc aatctatcag atgacctcgc tctagccttg gctgccaagg atgtccggat
     5341 tgaagcgcca atccctggga aatccctaat cggaattgaa gtgcccaact ccgatattgc
     5401 cactgtatct ttccgagaac tatgggaaca atcgcaaacg aaagcagaaa atttcttgga
     5461 aattccttta gggaaggctg ttaatggaac cgcaagagct tttgaccttt ctaaaatgcc
     5521 ccacttgcta gttgcaggtt caacgggttc agggaagtca gtagcagtta acggcattat
     5581 tgctagcatt ctcatgaagg cgagaccaga tcaagttaaa tttatgatgg tcgatcccaa
     5641 gatggttgag ttatctgttt acaatgatat tccccacctc ttgattccag tcgtgaccaa
     5701 tccacgcaaa gccagcaagg ctctgcaaaa ggttgtggat gaaatggaaa accgttatga
     5761 actctttgcc aaggtgggag ttcggaatat tgcaggtttt aatgccaagg tagaagagtt
     5821 caattcccag tctgagtaca agcaaattcc gctaccattc attgtcgtga ttgtggatga
     5881 gttggctgac ctcatgatgg tggccagcaa ggaagtggaa gatgctatca tccgtcttgg
     5941 gcagaaggcg cgtgctgcag gtatccacat gattcttgca actcagcgtc catctgttga
     6001 tgtcatctct ggtttgatta aggccaatgt tccatctcgt gtagcatttg cggtttcatc
     6061 aggaacagac tcccgtacga ttttggatga aaatggagca gaaaaacttc ttggtcgagg
     6121 agacatgctc tttaaaccga ttgatgaaaa tcatccagtt cgtctccaag gctcctttat
     6181 ctcggatgac gatgttgagc gcattgtgaa cttcatcaag actcaggcag atgcagacta
     6241 cgatgagagt tttgatccag gtgaggtttc tgaaaatgaa ggagaatttt cggatggaga
     6301 tgctggtggt gatccgcttt ttgaagaagc taagtctttg gttatcgaaa cacagaaagc
     6361 cagtgcatcc atgattcagc gtcgtttgtc agttggattt aaccgtgcga cccgtctcat
     6421 ggaagagctt gagatggcag gtgtcattgg tccagctgaa ggtaccaagc caagaaaagt
     6481 attgcagcaa taaaaaatag cttctttcca aggttggaag gagctatttt actggctatt
     6541 gattactttt atactcttcg caaatctctt caaaccacgt cagcttcgcc ttgccgtact
     6601 caagtactgt ctacggctag cttcctagtt tgctctttga ttttcattga gtattatttt
     6661 ctgaagttgg agtattgggc tgtttttcat tttcagtggc aggtttactt gtagcaggtg
     6721 tagtagagtc ctgagttgct gttttctgat cttctttttt ctcttccttg acgctagatt
     6781 ttggcgtttc ttcttgttgt gttttttctt gagaagtgtt attttcctta tttggagtag
     6841 tattttcctg aggggattct ttttgaattt ctttgacaaa ggttgtcgtc tggcttgtcg
     6901 taggttcttt tttagtattt ttattattat tatccaaggc gttcatgatc gtgatacccg
     6961 cggtaatcaa accaaggata ctaccgatag tagtaatggt tttttgaaaa acagtaaagc
     7021 cttttttgat atgttctgtt ttcggttttg aagttctacg atagttagac atacattctc
     7081 tcctttgatt atgatttatt atactctgtt tctttaaaaa aatcttaaaa aaagagaaat
     7141 tgccctttct tgtcgcaggc tccgtttcat gatacaatag aaggagtgat tttagaaagc
     7201 gtgagaaaca tgatttactt tgataattcg gcgacgacca ggccctatcc agaagccctt
     7261 gaaacctata tgcaggtggc ttcaaaaatt ttaggaaatc catctagtct ccatcgtttg
     7321 ggagaccagg caacacgaat tttagatgct tcccgtcaac agattgcaga tttaatcggt
     7381 aagaaaagtg atgaaatctt ctttacatct ggtgggacag aaggggataa ctggatcatc
     7441 aagggagtag catttgaaaa agcccagttt ggcaagcaca tcatcgtatc agccattgaa
     7501 catccagcag ttaaggaatc agccctctgg ttgaaagctc aaggttttga agtggatttt
     7561 gctccagttg ataacaaagg atttgtggat gttgaggcgc tagaagattt gataagacct
     7621 gatacgaccc tcgtttccat catggctgtg aacaatgaaa tcggctctat ccaacctatt
     7681 gaggctattt cagaattctt ggcagacaag ccaactattt ccttccacgt tgatgcggtt
     7741 caggcgcttg ccaagattcc gactgaaaag tatctgacag aacgagtgga tttcgcgact
     7801 ttctctggtc acaagttcca cggaatccgt ggtgttggtt ttatctatat caagtctggc
     7861 aagaagatta cgcctctttt aacaggtggt ggtcaggaac gtgattaccg ttcgacaact
     7921 gaaaatgtgg caggaattgc agcgaccgct aaggctctcc gtttatctat ggaaaagcta
     7981 gatatcttta ggagcaagac tgggcagatg aaggcagtga ttcgtcaagc acttctggac
     8041 tatccggata tatttgtctt ttcagatgag gaagacttcg cccctcatat tctgactttt
     8101 ggaatcaagg gtgttcgtgg tgaggtcatt gttcacgcct ttgaagacta tgatattttc
     8161 atctcaacga cctcggcttg ttcgtccaag gctgggaaac cagccggaac cctgattgct
     8221 atgggagtgg acaaggataa ggctcagtca gctgtgcgtc ttagtctaga ccttgaaaat
     8281 gatatgagtc aggtcgagca gttcttgacc aagctaaaat taatttacaa tcaaactaga
     8341 aaagtaagat aggagcattc atgcagtatt cagaaattat gattcgctac ggtgagctgt
     8401 caaccaaggg caaaaatcgt atgcgtttca tcaataaact tcgcaataat atttcagacg
     8461 ttttgtctat ctatccccaa gttaaggtaa cagcagatcg cgaccgtgcc cacgcttacc
     8521 tcaatggagc agattacaca gcagtagcag aatcgctcaa acaagtattt ggaattcaaa
     8581 acttttcccc tgtttataag gttgaaaaat ctgtagaagt tctgaagtct gctgtccaag
     8641 agattatgca ggacatctac aaggaaggca tgacctttaa aatctctagc aagcgtagcg
     8701 accacacctt tgaattggac agtcgtgaac tcaaccaaac gcttggaggg gctgtattcg
     8761 aagccattcc aaacgtacaa gctcaaatga aaagtcctga catcaatctt caggtggaga
     8821 tacgtgaaga agcagcctat ctttcttatg aaaccgttcg aggggcaggt ggattaccag
     8881 tgggaacttc tggtaagggc atgctcatgt tgtcaggggg aatcgattcc cctgtagctg
     8941 gctatctagc gcttaaacga ggggtagata tagaggcagt tcactttgcc agcccacctt
     9001 acacgagtcc tggtgctctt aaaaaagccc aagatttgac ccgtaaatta accaaatttg
     9061 ggggaaatat ccagtttatc gaagttcctt ttacagagat tcaagaggaa atcaaagcca
     9121 aagcgccaga agcctacctt atgaccttga cgcgtcgttt tatgatgcgt attactgacc
     9181 gtattcgtga ggtacgaaat ggtttagtta tcatcaatgg ggaaagtcta ggtcaagtag
     9241 ccagccaaac ccttgaaagt atgaaggcta tcaatgctgt taccaacact cccatcattc
     9301 gtcctgtggt taccatggac aagttggaaa tcattgacat cgcccaggaa atcgatacct
     9361 ttgacatttc aatccaaccg tttgaagact gttgtactat ttttgcacca gatcgtccaa
     9421 aaacaaatcc taaaattaag aatgcggagc agtacgaagc gcgtatggat gttgaaggct
     9481 tggttgagcg agcagtggct ggaatcatga ttactgaaat cacacctcaa gccgaaaaag
     9541 atgaagttga tgacttgatt gacaatctgc tctaattcag aaaatccaaa agaatagcga
     9601 aaatcagtaa aaaaagttag ttttttctct aaaaacaggt aaaaaactaa ctttttttat
     9661 ttttatgata taatgatata aaattttgaa tatagagagt tttctgacaa tgaatcaatc
     9721 ctacttttat ctaaaaatga aagaacacaa actcaaggtt ccttatacag gtaaggagcg
     9781 ccgtgtacgt attcttcttc ctaaagatta tgagaaagat acagaccgtt cctatcctgt
     9841 tgtatacttt catgacgggc aaaatgtttt taatagcaaa gagtctttca ttggacattc
     9901 atggaagatt atcccagcta tcaaacgaaa tccggatatc agtcgcatga ttgtcgttgc
     9961 tattgacaat gatggtatgg ggcggatgaa tgagtatgcg gcttggaagt tccaagaatc
    10021 tcctatccca gagcagcagt ttggtggtaa gggtgtggag tatgctgagt ttgtcatgga
    10081 ggtggtcaag ccttttatca agcacaaaac aggttggttt gatggcatga tgacgacagg
    10141 ttgctctatg ggagcctatc atgcactcaa tttcttcctc cagcatccag atgtctttac
    10201 caaagtgatt gctctcagtg gtgtttacga cgcacgtttc tttgtcggcg attactacaa
    10261 cgatgatgct atttaccaaa actcgccagt agattatatt tggaaccaaa acgatggctg
    10321 gtttattgac cgttaccgtc aggcagagat tgtgctgtgt acggggcttg gagcctggga
    10381 acaagatggt ttaccatcct tttacaagct caaagaagcc tttgaccaga aacaaattcc
    10441 agcctggttt gctgaatggg gacatgatgt cgcccacgac tgggaatggt ggcgtaaaca
    10501 aatgccttat ttcctcggta atctctattt ataaaaggag ttacctatga attaccttgt
    10561 tatttctccc tactatccac aaaactttca acagtttacc atcgaactag ctaataaagg
    10621 catcacagtc ttgggaattg gtcaagagtc ttacgagcaa ttggatgagc ccttgcgcaa
    10681 tagcttgacc gagtattttc gtgttgataa tcttgagaac atagatgaag tcaaacgtgc
    10741 agttgctttt ctcttttata aacatggtcc aattgaccgc atcgagtctc acaatgaata
    10801 ctggcttgag ctagacgcaa cactcagaga acaattcaat gtttttggtg ccaaaccaga
    10861 ggatctcaaa aagacgaaat ataagtctga aatgaagaaa cttttcaaaa aagcaggtgt
    10921 tcctgtggta cctggagctg ttatcaagac ggaagcagat gttgatcaag cagtgaaaga
    10981 aatcggtctt ccaatgattg ccaaacctga taatggagtg ggagcagccg caacctttaa
    11041 acttgagaca gaagacgata tcaatcactt caagcaagaa tgggaccatt caacccttta
    11101 tttctttgaa aaatttgtca cttccagcga aatctgtacc tttgacgggc tcgtggacaa
    11161 ggatggaaag attgtcttct caacaacctt tgactacgcc tatacaccgc ttgacctcat
    11221 gatttataag atggacaatt cttattatgt gctcaaggat atggatccta aactgcgcaa
    11281 gtatggggaa gcaattgtca aagaatttgg tatgaaagaa cggtttttcc atattgagtt
    11341 cttccgtgag ggggacgatt atattaccat cgagtacaat aaccgccctg caggtggttt
    11401 taccattgat gtttataact ttgctcattc cttggacctt tatcgtggct atgcagctat
    11461 tgtcgcagga gaggagttcc cagcgtcaga gtttgaacct cagtattgtt tggctacatc
    11521 acgtcgtgca aatgctcact atgtctattc tgaggagaac ttacttgcca aatatagcca
    11581 gcagttcaag gttaaaaaaa tcatgccaga ggcttttgcg gaacttcaag gagattactt
    11641 ttatatgctg acaactccga gtcgacaaga aatggatcag atgattgctg atttcggaca
    11701 acgtcaagaa taagaactat cggattaagg aaattcactc ccttaatcct tttgttttgt
    11761 ctggtaaaaa ataagagcat cctaacaagg tagctatcat aaaacttgtt cgataactat
    11821 ttgaagcagg attaggtggc tagaaattga attttaacat ttcaatttag tcatagtatt
    11881 gtgttttcgt atccttaaat cagctaaaag gatccatgac gacacctata cgatatagtt
    11941 ttcaggatac caaacaagtc tattaatact caatgaaaat caaagagcaa actaggaagc
    12001 tagccgcagg ttgctcaaaa cgctgttttg aggttgtgga tagaactgac agagtcagta
    12061 tcatatacct acggcaaggt gaagctgacg tggtttgaag agattttcga agagtataaa
    12121 atattcaggt gacgcataga tatagttaat tgaagctttg tttgaaatct gataaaataa
    12181 tgatattact aagttttaaa aactaaagaa aagggaagat atgattacag gcgaattaaa
    12241 aaataaaatc gatcagttgt gggaaattct ttggacagaa ggaaatgcaa atcctttaac
    12301 aaatattgaa cagttgactt atctcttatt tatgaaagat ttggatagtg tcgagcttgg
    12361 acgtgaaagt gatgctgaat ttctagggat tccttatgag ggagtttttc caaaagataa
    12421 acctgaatac cgttggtcaa cttttaaaaa tataggagat gctcaggaag tttatcgttt
    12481 aatgactcag gagatttttc cgtttattaa aaatctcaag ggggatacag atgatacagc
    12541 cttttcacga tatatgcgag aagctatttt tcaaataaat aaacctgcta cgcttcaaaa
    12601 ggcaatttct atcttagatg tttttccaac taggggatta gatgtagatt ttgataatga
    12661 caaacaaagt attactgata tcggagatat ctatgaatat ctgttatcaa aattgtcgac
    12721 cgcaggtaaa aatggacagt tccgtacacc tcgtcacatc atcgatatga tggttgagtt
    12781 gatgcaaccg actatcaaag atatcatctc agatcccgct atgggttctg ctggcttctt
    12841 agtatctgct agccgttact taaagcgtaa gaaagatgaa tgggaaacca atacagataa
    12901 tatcaatcat tttcataatc agatgtttca tggaaatgat acggatacga ctatgttgag
    12961 acttggggcg atgaacatga tgctacatgg agtagaaaat ccacaaatca gttaccttga
    13021 ctcgctgtct caagataatg aagaagccga taaatatact ttggttttag caaatcctcc
    13081 ttttaagggc tcacttgact acaattcaac ctctaatgac cttcttgcaa ccgtaaaaac
    13141 caaaaaaaca gaattactct ttctttctct tttcttgcga actttaaaac caggtggacg
    13201 agcagcagtt atcgtacctg atggtgtcct ttttggttcg tctaaagctc ataaaggaat
    13261 tcgtcaggaa attgtagaga atcataagct tgatgctgta atctcaatgc ctagtggtgt
    13321 tttcaagcct tatgctggag tttcaactgc cattctcatc tttacaaaaa ctggtaatgg
    13381 tggtactgac aaagtctggt tttacgatat gaaagcggat ggtttaagtt tggatgataa
    13441 gcgacaaccg attagcgaca atgatattcc agatattatc gaacgctttc atcatcttga
    13501 aaaagaagca gaacgtcaga gaacggatca atctttcttt gttccagttg ctgagataaa
    13561 ggaaaatgat tatgatttgt ctatcaataa atataaagag attgagtatg aaaaagttga
    13621 gtatgaacca acagaggtca tcttaaagaa aatcaatgat ttagaaaaag aaattcaagc
    13681 tggcttggct gaattagaaa aattactcaa gtagggaggt ggctgtatga aaaaagtgaa
    13741 gttgggggaa gtcttatctc taaaaaaagg caagaaagcc actgtacttg ctgaacaaac
    13801 aactctaagc caacgttata ttcaaataga tgatttaaga aataataata atttaaaatt
    13861 cactgaaagt ttaaatatga ctgaagcact cccagatgat attctgatag catgggatgg
    13921 agctaatgca ggaacagttg gttatggatt atcgggagct gttggtagta caattacggt
    13981 cttaaaaaag aatgagcgat acaaagaaaa aattatatca gattacttgg gagtcttttt
    14041 ggaaagtaaa tcgcagtatt tacgagaaca ttcaacaggt gcaacaattc ctcatttaaa
    14101 caagaatata ttacttgatt tacaattaga attgctaggt atcgaagaac aagagaacat
    14161 tatctgtatt cttaatacga ttaaagggct tattactaaa agaaaattgc agttagatga
    14221 actaaacttg ctcgtcaaat cccgatattc cgaggaggtg gttgcatgaa gaaagtgaaa
    14281 ttagggcaag tggctacttt tatcaatggc tatgctttta aacctcaaga ttggtcatct
    14341 gaagggaaag agattatcag aatacagaat ctgactaaaa cttcaaaagg gataaattat
    14401 tattcgggta cgattgataa aaaatacatt gttgaagctg gtgatattct tatttcgtgg
    14461 tcgggtacat taggggtttt tcagtggtgt ggaagatccg cagtattaaa tcagcacatt
    14521 tttaaagtgg tttttgataa gatatatata gataaatcgt attttaaata tgttgttgaa
    14581 aaaggtttac aagatgctgt aaaacatact catggttcca caatgaaaca tttaactaaa
    14641 aaatattttg ataatattat agttccatat acaaatttgg gagagcaaca aagaattgca
    14701 agtgagctgg atttgttaag caagctgatt ttaaggagac aagaacaact tgaagagctt
    14761 aatctacttg taaaatcccg atttaacgag atgtttgggg atcctttaaa taataacaag
    14821 aaatttgcag taaaaacggg acaacagtgt tttaaatttt caagtggaaa atttcttgat
    14881 aaacatgata gagtttttga aggttatcct gcctatggtg gaaatggcat tgcttggaaa
    14941 agtcgaaaat atttaattga taatcctact atcatcattg gtcgtgtagg tgcatattgt
    15001 ggtaatgtaa gaactaccca cggtaaggta tggataagtg acaatgctat atatataaaa
    15061 gaatttaaga attccgattt caatcttgta tttttattag aattaatgaa agtaattgat
    15121 tttagtaaat ttgctgattt ctcaggtcaa ccaaagataa ctcaaaaacc attagaaaat
    15181 cagaaatata tcctcccccc tctagccctc caaaatgagt tcgcagactt tgtagccctg
    15241 gtcgacaaat cacaatttgc ttgtgagata gctataaaag tgtggagaaa tagcttgaaa
    15301 tttagtataa tatagctaaa ctatttgttt aaagtgagaa aaaaatggga aattttagct
    15361 ttcttttaaa aaatgacgaa tatgaatctt tttcaaaacc ttgcattgaa gctgagaata
    15421 taattgctac atcaactgtg gctactgcct ttatggcgcg tcgtgcttta gagcaggctg
    15481 tccattggat atatagtcac gattcatatt tagaagctcc ctatcgtgct actctatctt
    15541 ctttagtatg ggatgatgat tttagggata tcgtagattc tgaactccac aagcagatag
    15601 ttctgttgat tcggtgggga aaccatgctg ctcatggtgg tgaaattaag gaacgagaag
    15661 cgattttagc tttgcatcat ttgtatcagt ttgttaattt tatcgattat tgttacagca
    15721 atgagtttgt ggagcgttat tttgatgaga agtgcttacc actttcagca aacatcaaat
    15781 accgagaaac tccacaatct atgataaagt tacaagacag tttaccagaa ctgcctgatt
    15841 ttcatgaaca gatggctgct cagtccgtag aagttcaaga gacttatact gaaaaacgtg
    15901 agactgcagc gcaacggcaa gatgtgcctt tccatattga tcaattatct gaggcagaga
    15961 caagaaagct ctttattgat atcgatctcc gtttagcagg atggatattt gaagaaaact
    16021 gtcgtgttga gatagccgtt gatggtctca agcacggttc aggaattggt tactgtgact
    16081 atgtacttta tggtaaaaat gggaaaattt tagcgattgt ggaggctaaa aaagcctctg
    16141 tcaatccaga agtaggggaa gtacaggtca aagaatatgc tgaagctttg gagaaacata
    16201 tcggctatca gccaatttgc tttattacaa atgggttgaa gcactatata cttgatggtc
    16261 cgaaccgccg ccagattgca ggcgccagat tgcaggcttt tactctcaag aagaattgca
    16321 attagtgatg gatagacgtc atcttcaaaa accgcttgag gatatttcta gtaaaattag
    16381 ggacgatatt tccgggcgtc actaccaaaa acatgccatt gcaagcgttt gtgaagcttt
    16441 ctctgatcat cgtagacagg cacttttggt tatggcaact ggggcgggga aaactcgtac
    16501 agcagtttct ctagttgata tcttatcacg tcataactgg gtaaaaaacg ttctcttctt
    16561 agccgataga acttccttgg ttaagcaagc ctatgattcg tttagaaaat tactcccaga
    16621 tctttccgtt tgtaacttct tagaagataa agaaggagct caatcaagtc gtatggtctt
    16681 ttcaacttat ccgaccatga ttggagcgat tagtggtcaa gaagaagtaa atcaacgccc
    16741 ttttactgtt gggcattttg accttatcat aattgacgaa tctcaccgtt ctatttatca
    16801 gaaatacaag tccatttttg attattttga tgcaagaatt gtaggcttaa cagctactcc
    16861 gcgtcaagat ttagataaaa acacctatgg attctttaat ttggagaatg gggttccaac
    16921 atatgcatat gatttggaag aggctgttaa agacggatat ttagtagcct atcattctat
    16981 cgaaaccaaa ctgaaactac ctacggatgg tctacattat gatgatttgt ccgaagaaga
    17041 aaaggaacat tttgatagca aatttgaaga cgatagctgt gaaaaagata ttgatgggag
    17101 tgtgtttaat tcctttattt tcaataaaag tacagtagaa attgttttaa atgaactcat
    17161 gacaagagga attcagacag cctcgggtga tgaaattggt aaaactatta tttttgctaa
    17221 aaatcatgac catgcggaat atatcagagg tatttttaac aaccgctatc ctgaaaaagg
    17281 gagcgactat gctcaggtga ttgattatag tattaagcat tatcagacct tgattgatga
    17341 ttttaaaatt aaggagaagt atcctcaaat tgcgatttct gtcgatatgt tagatacagg
    17401 tattgatgta ccagaggttg ttaatttagt cttcttcaag aaagtacgct ctaaaactaa
    17461 gttttggcag atgattggtc gaggaacccg tctatgtaaa gatttatttg gacctgagca
    17521 ggataaggaa aacttcttgg tatttgatta tggggacaat tttgattatt ttcgtgcaga
    17581 tccaagagat ggagagggtc gtcacattgt ttcgctgact cagcgtttat ttaatatcaa
    17641 agtggacttg attcgagaac ttcagggact ccaataccaa gaagatcagt ttgcgagagc
    17701 ataccgtcag cagcttgtct cggaacttca aggtcgtata gagagcttaa atgagttgga
    17761 cttcagggtt cgtatggttt tagatacagt ttatagctat aggaaattgg aaagttggca
    17821 gaatctaact gctgttacaa gtgaaaccat tcaaaaaaat ctctctctgc ttttatttga
    17881 tgaagataaa gaagatgaga tggcgaggag atttgatttg tggttgcttc atattcagtt
    17941 ggggcaactg acagctaaat cttccactgt tcatatttcc caagtgatga agacggctag
    18001 agctctttct gctattggca atatcccgca ggtttttgag caggctgaaa ttatcaggaa
    18061 agtacaggag cctgaatttt ggaaagaagt taacttgtct gatttggaaa aaattcgtct
    18121 tgctattcga gatttattac agtttttgga taaaacagac cgtaaaccct actatgttaa
    18181 ctttgaagat cgtatactct ccactgttca cgagaccaca gcatttttgc aggtcaacga
    18241 tcttcggtct tacaatgaaa aagttgagca ttatttgaaa actcatctgg atgaggagtc
    18301 catttctaag ctataccata ataaaaagtt gacatctgat gatatgcttg cacttgaaaa
    18361 attgctttgg gaaaaattag gtagtaaagc agactaccaa agtcattatg aaaataaggc
    18421 aattccgaga ttggttcgtg agattattgg cttagataga gagtctgcca atcgtatttt
    18481 ttctaaattt ttgtcggatg agaatcttaa tgccaggcag atttcatttg taaaattgat
    18541 tgtagactac attgtagaaa atggtttttt agagacgaaa gtgttaacgc aagagccgtt
    18601 taaatcttat ggttctgttc aactactctt ccaacaccaa ctaccagtac ttcgtaatat
    18661 tgttcaaatc attgaactta tcaataatcg agctggagaa gcggcttaaa ttctaaagtg
    18721 attgccatgc tgagactcat ttaaaattaa aaagagtaga aatttatgct atatatgaga
    18781 agttttatta ggaagaatgt catcgttttc ctagaataca gtatcagttg ttaagtggtt
    18841 gataaatttc aaagtagata cttgtaccac gatgtttgtt gatcgagtta ttaacaaaag
    18901 agctactttg attttaaaga aatagaaaac aaaaagccga gcaagaattc aattgcagga
    18961 gaaaatgaaa taatactcaa tgaaaatcaa agagcaaact aggaaactag ctgcaggctg
    19021 ctcaaaacac tgttttgagg ttgtagatgg aagctgacgc ggattgaaga gattttcgaa
    19081 gagtataaat cttcctagga taaagcaaaa cgcatagtat caagggtttt caacacttga
    19141 tactatgcgt tttctgatgt taaagacttt ctaccaggtt ttttaaaagc ataattgtta
    19201 gttgtagtca tttattattc ttcaaagaaa aatggtgggg cgaatttttt cagttcttca
    19261 aagcactttt gagcagtatc tgcatcttca cagatgataa gacagacatc attaccacaa
    19321 agggtagcga tagcgtcagg gaagctcaaa gtatcaatga tagaaccaaa ggattgagcc
    19381 agtccaggaa gggtttttag taggacttgg tgttgaactg ggcgcatcca gacaagggcg
    19441 tcttccatgt agagttcgag acgtttttcc cattttgaga tggaaccatt gttaagaaca
    19501 taataagcgc tatcttcttc gcggactttt gataggttca tatttttgat gtcgcgtgag
    19561 agggttgcct gggttacttg aatgtcgttc tcagcaagaa gggcttgcaa ctcagcctgt
    19621 gtatgaatct tgttttttgt gataagagcg cgtataagtt ggtggcggtg ttctgattta
    19681 ttcataataa tgtaactcct tttagcaagg taaggtaagc atggactgag cgaggtcgac
    19741 agtcaagtgg tagtctgtat tgtcacggat ggtgatttca aagtcagtag tatagaggac
    19801 taaacggaga gtgtctcctt cttttagctt gtaaatagtt ggctgcagtt caaattgaac
    19861 gtccatccat tcatctgcag taatatcctc tactaacagt aaatcatttc tattttgtaa
    19921 attaaggtaa ccttttgtca cgactcgttg tgcctctggt ctaaatggca attcacagag
    19981 attttccaac atgtgatagc gaccgttgtc aatggttcta gcacttaaaa tagctggata
    20041 aggttgtagg tatttctttt gcccaaattc tagcagttgg gcagataaga gccccttgtt
    20101 tgtactggat ttgatacgaa gattgagctg agcgcgaccg tttaggtgga gatctttagt
    20161 cacaggaagg ttaatagtaa tctgattggc tttcccttga tagagctctg tattgaaggt
    20221 ttggtatgtc ttaccatagc gctcaaaatc cttatctggg tactggtttt gaatagcttg
    20281 ctcttcttga ccaagtgaga aggtttcaca gttttcttgc ccaccgaagt tatcaagtga
    20341 taaccaagtc tgtggagctg tattgtcctg ccagataaca gtaggaagtt gaaagtctgt
    20401 ttcctgtcct agtaatttct tggtcaataa ggcatttatg gactcacgga agtcaattga
    20461 ttgccaattg ttcatgtaaa catgggcacc attatggaaa aagagatgct tgtttatatg
    20521 agtaggaaga gcatggaaca tctggtaaac atgaagtggt ttgacattcc aatcctgaga
    20581 accatgagta aagacaacct ctgcctttac tttatgggca ttgagcagat aattgcggtc
    20641 atgccaaaac tgattgtagt ccccagtttt tcggtctagc tgagctttca ctttttctaa
    20701 gtcagcttgg tgagcttcat tgccacggat atagtcgcca gctaagagat tacgagaata
    20761 ggttaactca gcaagggagt caaagtcctc acctggataa ccacctgggc tagtcaccag
    20821 accgttttca cggtagtagt tgtaccatga tgaaattcct gcctcggcaa tgataacttc
    20881 taaaccatcg actcctgtag tcgcaagacc attggacatg gtacctagat aggaaagtcc
    20941 tgttgtagca acttttccgt ttgaccaatc agccttgact tgacgctggc gcgtgtgatc
    21001 agtaaaggca cggcaacggc cgttaagcca atcgatgaca tttttataag cctcgatttg
    21061 ctggtagtct ccattagtca tgaaacctgt cgagtctttg gtaccaacac ctgagacata
    21121 gagattggca aagcctctcg gaaggaagta gtcgtttagt gtatagctag agttgatgtg
    21181 agttagcttt tcctcagcct ctgctataag ctcagcttta ccttggggtt ggacgagatt
    21241 tagttgaggt ttctctagct caatcttgtg aggaagctta acctcaagct cgccctccat
    21301 cttgtagaga gccttgtcac tagccttgtc attggttccc tgatgataag ggctggctgt
    21361 catgatggca gggatttttc catcaaaacg agggcgaata atgctaacct ttactaggtc
    21421 tgatagccct ttttggtcag tatcgacacg agactcaacg taaacgactt cacgaatgac
    21481 atcctggtta gaaaaagtag ccaaactctt gccgttaaag tagtggtagt cattatcctc
    21541 cggaataaga ccatcactaa caagttggtc gataagagta tttccttttt tggtgcgagt
    21601 attgagtaac tgatagagat tttcaatcaa gtcaccatat ataatgggaa atccagtttc
    21661 tttacgaaaa acgtcactat cttcgaagtc aaccaaataa gaaaagccta aaagttgaaa
    21721 agcaacagta taaaaaatat ctgctgtcag ttcatcttct gattgaaaaa atgtcagcag
    21781 gtctgttttt ttatcagctg ctaggataga aagtgggtag ttggtgtctt gataagtgaa
    21841 aaagaaacga cgtaaaaagg tttcaagtga gtctttgtga ttggctgtat tttgtaaatc
    21901 aaagccacat ttttttagtt cagataagac attttctttt ggaaaattga tataactata
    21961 ttgattaaaa cgcatagaac ctccatatag aatgacagtt aaggttatta tatcaaaaaa
    22021 aaagcagaaa gggaattgtt aacttcaaaa ggaaataatc caataaaa
//
LOCUS       DAVKJD010000026        21922 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKJD010000026 DAVKJD010000000
VERSION     DAVKJD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.026"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(1..188)
                     /locus_tag="VW157_001030"
                     /pseudo
     CDS             complement(1..188)
                     /locus_tag="VW157_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            179..577
                     /locus_tag="VW157_001031"
     CDS             179..577
                     /locus_tag="VW157_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9552346.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQSLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(774..1694)
                     /gene="cysK"
                     /locus_tag="VW157_001032"
     CDS             complement(774..1694)
                     /gene="cysK"
                     /locus_tag="VW157_001032"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9552347.1"
                     /translation="MSIYNSITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1979..2614)
                     /locus_tag="VW157_001033"
     CDS             complement(1979..2614)
                     /locus_tag="VW157_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9552348.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            2670..3968
                     /locus_tag="VW157_001034"
     CDS             2670..3968
                     /locus_tag="VW157_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9552349.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLDLYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3965..4627
                     /locus_tag="VW157_001035"
     CDS             3965..4627
                     /locus_tag="VW157_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9552350.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            4707..5255
                     /gene="raiA"
                     /locus_tag="VW157_001036"
     CDS             4707..5255
                     /gene="raiA"
                     /locus_tag="VW157_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9552351.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            5391..7364
                     /locus_tag="VW157_001037"
     CDS             5391..7364
                     /locus_tag="VW157_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9552352.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDHVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            7361..7813
                     /gene="rplI"
                     /locus_tag="VW157_001038"
     CDS             7361..7813
                     /gene="rplI"
                     /locus_tag="VW157_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9552353.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7857..9209
                     /gene="dnaB"
                     /locus_tag="VW157_001039"
     CDS             7857..9209
                     /gene="dnaB"
                     /locus_tag="VW157_001039"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9552354.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            9211..9477
                     /locus_tag="VW157_001040"
     CDS             9211..9477
                     /locus_tag="VW157_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552355.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            9566..10912
                     /gene="cbpD"
                     /locus_tag="VW157_001041"
     CDS             9566..10912
                     /gene="cbpD"
                     /locus_tag="VW157_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9552356.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGHSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10995..11097
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11168..11458
                     /locus_tag="VW157_001042"
     CDS             11168..11458
                     /locus_tag="VW157_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9552357.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVPMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            11421..12179
                     /locus_tag="VW157_001043"
     CDS             11421..12179
                     /locus_tag="VW157_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552358.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            12218..13225
                     /locus_tag="VW157_001044"
     CDS             12218..13225
                     /locus_tag="VW157_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9552359.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            13225..13953
                     /locus_tag="VW157_001045"
     CDS             13225..13953
                     /locus_tag="VW157_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552360.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            14072..14530
                     /locus_tag="VW157_001046"
     CDS             14072..14530
                     /locus_tag="VW157_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9552361.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            14532..16964
                     /locus_tag="VW157_001047"
     CDS             14532..16964
                     /locus_tag="VW157_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9552362.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAEV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKSKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            17606..18259
                     /gene="rr06"
                     /locus_tag="VW157_001048"
     CDS             17606..18259
                     /gene="rr06"
                     /locus_tag="VW157_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9552363.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            18256..19596
                     /gene="hk06"
                     /locus_tag="VW157_001049"
     CDS             18256..19596
                     /gene="hk06"
                     /locus_tag="VW157_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9552364.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            19679..20206
                     /locus_tag="VW157_001050"
     CDS             19679..20206
                     /locus_tag="VW157_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9552365.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            20285..>21922
                     /gene="pspC"
                     /locus_tag="VW157_001051"
     CDS             20285..>21922
                     /gene="pspC"
                     /locus_tag="VW157_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HEU9552366.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVAVASLFMGSVVHATEKEGST
                     QAATSSNMAKTEHRKAAKQFDEYINKMIQLDRRKDTQNRTLIIKLSAIKTKYLRELNV
                     LEEKSKDELPPKIKEELTAAFEQFKKDTLPTEPGKKVAEAKKKVEEAEKKVAEAKKKA
                     KAQKEEDYRNYPTITYKTLELEIAEFDVKVKEAELELVKKEADESRNEGTINQAKAKV
                     ESEKAEATRLKKIKTDREKAEEEAKRRADAKEQDESKRRKSRVKRGDLGEQATPDKKE
                     NDAKSSDSSVGEETLPSPSLKPGKKVAEAQKKVEEAKKKAKDQKEEDRRNYPTNTYKT
                     LELEIAESDVKVKEAELELVKEEAKESQNEEKIKQAKAKVESKKAEATRLENIKTDRK
                     KAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPKPEKPAEQPKAEKTDDQQAEE
                     DHARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNN
                     GSWYYLNSNGAMATGWLQNNGSWYYLNANGSMATGWLQ"
BASE COUNT         6921 a         3644 c         4960 g         6397 t
ORIGIN      
        1 gctttattct taaatccacg tttacgaatc tctttccaca cttgttcaat ggggttcatc
       61 tctggtgtgt atggaggaat aaatgcaaag ccaatattag tcggaatctt taaggtactt
      121 gatttatgcc atatagcatt gtccataacg agtaaaagat aatcatctgg ataagcttgt
      181 gaaatctcct attcctaaag cccctttagc gcataacttt ggctcagctt ctattatcgc
      241 tcacaccatc catcagaagt ttaatctgaa ggtacccaat tatcgccaag aagaagattg
      301 ggctaggatg ggtttaccaa tcacacgtaa ggaaatctct aatcggcata tcaagacgag
      361 tcaatactat ttggagcccc tttataacct cttgcgagag agactattga ctcagtcctt
      421 acttcatgcg gatgaaactt cttatagggt gctagagagt gatagtcagc tgacttacta
      481 ttggactttt ttgtctggga aagctgagaa acaagggatt acgctttacc atcatgtcct
      541 aatcgatttg ttcatatctt atttcaatcc attataaata gtgagaaata tctatcctat
      601 cttctagaat gtcttccaaa cgtggaaact ctcgtaaaga aagaggtttt agaggcctat
      661 ttaccatgga ctaaagttgt acaagaaaag tgcaaataag aaatctccag attaggaact
      721 atccgtgagt tctctagtct ggagattttt caatagactt cgttattgga cggttacaat
      781 tcataaagtg ctgtagagag ataacgttca ccgttatctg gtgctagggc aaggactttt
      841 ttacctgtac ctaatttttt ggcaacctcg atggctccgt agatagctgc agctgaggaa
      901 atccctacaa ggaagccttc ttttccacca atttcacgtc cgagtgcaag agcgtcatct
      961 gatgttacac gaacgatacc atcataggct ttagtatcaa gtgtatcagg aataaatcca
     1021 gctgagatac cttgaatttt gtgaggacca ggtttttcac cagatagaat agcagattca
     1081 tctgcttcta ctgcaaaaac ttgaatgtta gaattttctg atttgagtgc atgagaaaca
     1141 ccagaaatcg ttccaccagt acctactcca gcaacaaagg catctaatcc atctttaccg
     1201 aaagcagcta gtatctcagc tcctgttgtt ctttcgtgta cttctggatt agctggattg
     1261 tcaaattgaa gaggaaggaa accatcacgt tcagcagcga tttcttgagc cttagcaata
     1321 gcacctttca ttccctcgct accaggagtt aggacgagtt cagcaccata agcttggata
     1381 attttacgtc gttctacact catagtttca ggcataacga tgacgacttt atacccttta
     1441 gcagcaccta cccatgaaag tccaataccg gtgtttccac ttgttgcttc aacaatagta
     1501 gaaccaagtt tcagaatacc atcttgttca gctttttcaa tcatgctaag ggcaatacgg
     1561 tcttttacag atgaaccagg attaaatgct tcaagcttta tatagacgtc tgcagcacct
     1621 tctggcacga tgttgttaag tttaacaatc ggtgtttgac cgattaattc agtaatgctg
     1681 ttataaatag acatatgaat acctctttgt ataagatatc tttagtataa cgctatccat
     1741 accatttgta aaatatagaa atcctatcga agcgatagga tttctatata tcatagatca
     1801 aagtcattaa ttttcaagtg attgcgttca gttaattcta gtaaataggt gtgaaggaaa
     1861 acgatatcag gtttcgtttg aattgcttca ttttctagta tattttggta cgcatctttt
     1921 tattttgctc gctaaagtag ttgtgagaat gtcaacgaaa cgctgtatta ttcattgttt
     1981 acactaagtt tacaggaact tcaacctctc gtaaaccttg gtcagttaaa gtgacttttc
     2041 cattaaaaaa ctccacaagt gcagctttaa tagtttcttt ttcttcttta tcaacataaa
     2101 tcatcgtatc gacttgatct gtaaagtttg tatccagctc catgagatca tgttctttaa
     2161 ggaagttact gtactcttgg tactgagcat aagacatttg aatagcaatg ccagcctgtt
     2221 cttttatttc aataatacca atttctttga cagctaaggc gacactgccg gcgtaagcac
     2281 gaattagtcc tccagcgcct agtttaatac caccaaagta gcgtgtcacg accacacaga
     2341 cattggtgag attgtgattt tctagtaccc caagcatggg aacaccagca gtaccactag
     2401 gctcaccatc atcacttgta cgtttaattt cactacgttc tccaataata aaggcagagc
     2461 agttatgtgt cgctttgtag tgttcttttt tgatggcagt aatgaagtca cgagcctctt
     2521 cttcgctata aacacgcttg gcatggcaga taaagcgaga ttttttgatt tcttcttgga
     2581 cttgaccgtc ctctttaatt gtcctaaatt ccatgattta attgtactaa aaaatcatct
     2641 aaagtgctag tttttacgaa taaagaagta tgaaagtaaa tttagattat ctcggtcgtt
     2701 tatttactga gaatgaatta acagaagaag aacgtcagtt ggcggagaaa cttccagcaa
     2761 tgagaaagga gaaggggaaa cttttctgtc aacgctgtaa tagtactatt ctagaagaat
     2821 ggtatttgcc catcggtgct tactattgtc gagagtgctt gctgatgaag cgagtcagaa
     2881 gtgatcaaac tttatactat tttccgcagg aggattttcc aaagcaagat gttctcaaat
     2941 ggcgcggcca attaactcct tttcaagaga aggtgtcaga gggattgctt caagtagtag
     3001 acaagcaaaa gccaacctta gttcatgcgg taacaggagc tggaaagaca gaaatgattt
     3061 atcaagtagt ggctaaagtg atcaatgcgg gtggtgcagt gtgtttggct agtcctcgca
     3121 tagatgtttg tttggatctg tacaagcgcc tgcaacagga tttttcttgc gggatagctt
     3181 tgctacatgg agaatcggaa ccttattttc gaacaccact agttgttgca acaacccatc
     3241 agttattgaa gttttatcaa gcttttgatt tgctgatagt ggatgaagta gatgcttttc
     3301 cttatgttga taatcccatg ctttaccacg ctgtcaagaa tagtgtaaag gagaatggat
     3361 tgagaatctt tttaacagcg acttcgacca atgagttaga taaaaaggtc cgtttaggag
     3421 aactaaaaag actgaattta ccgagacggt ttcatggaaa tccgttgatt attccaaaac
     3481 caatttggtt atcggatttt aatcgctact tagacaagaa tcgtttgtca ccaaagttaa
     3541 agtcctatat tgagaagcag agaaagacag cttatccgtt actcattttt gcttcagaaa
     3601 ttaagaaagg ggagcagtta gcagaaatct tacaggagca atttccaaat gagaaaattg
     3661 gctttgtatc ttctgtaaca gaggatcgat tagagcaagt acaagctttt cgagatggag
     3721 aactgacaat acttatcagt acgacaatct tggagcgtgg agttaccttc ccttgtgtgg
     3781 atgttttcgt agtagaggcc aatcatcgtt tgtttaccaa gtctagtttg attcagattg
     3841 gtggacgagt tggacgaagc atggatagac cgacaggaga tttgcttttc ttccatgatg
     3901 ggttaaatgc ttcaatcaag aaggcgatta aggaaattca gatgatgaat aaggaggctg
     3961 gtctatgaag tgcttgttat gtgggcagac tatgaagact gttttaactt ttagtagtct
     4021 cttacttctg aggaatgatg actcttgtct ttgttcagac tgtgattcta cttttgaaag
     4081 aattggggaa gagaactgtc caaattgtat gaaaacagag ttgtcaacaa agtgtcaaga
     4141 ttgtcaactt tggtgtaaag agggagttga agtcagtcat agagcgattt ttacttacaa
     4201 tcaagctatg aaggattttt tcagtcggta taagtttgat ggagacttcc tgttaagaaa
     4261 agttttcgct tcatttttaa gtgaggagtt gaaaaagtac aaagagtatc aatttgttgt
     4321 aattccccta agtcctgata gatatgctaa tagaggattt aatcaggttg agggcttggt
     4381 agaggcagca ggctttgagt atctggattt attagagaaa agagaagaga gagccagttc
     4441 ttctaaaaat cgttcagagc gcttggggac agaacttcct ttctttatta aaagtggagt
     4501 cactattcct aaaaaaatcc tacttataga tgatatctat actacaggag caactataaa
     4561 tcgtgttaag aaactgttgg aagaagctgg tgctaaggat gtaaaaacat tttcccttgt
     4621 aagatgagga aaaaatttgc aaaaatagaa tgaaagcgtt ataataaaat cataaaaaca
     4681 aaaatgttta gaaagaaggt actcatatga ttaaatatag tatccgtggt gaaaacctag
     4741 aagtaacaga agcaattcgt gattatgtag tttctaaact cgaaaagatc gaaaagtact
     4801 tccaaccaga acaagagttg gatgcccgaa ttaacttaaa agtttatcgt gaaaaaacgg
     4861 ctaaagtgga agtaacgatt ccgcttggat ctattactct ccgcgcagaa gatgtatctc
     4921 aagatatgta tggttcaatt gaccttgtaa ctgataaaat tgaacgtcag attcgtaaaa
     4981 ataaaacaaa aatcgagcgt aaaaataaaa ataaggtagc aactggtcaa ttatttacag
     5041 atgctttggt ggaagattca aatattgtcc agtctaaagt tgttcgttca aaacaaattg
     5101 atttaaaacc aatggatttg gaagaagcaa ttctacaaat ggatttattg gggcatgatt
     5161 tctttatcta tgtggatgtt gaagatcaga caaccaacgt gatttatcgt cgtgaggatg
     5221 gcgaaattgg tttgttagag gttaaagaat cttaatttca atatgtgtaa aggtaggttt
     5281 actgaattgt aaactacctt tttcttataa ttgatagaat tactgattac acttttaaaa
     5341 agtcgctttt tgaggggtat tatggtataa tgggtgccaa gaggttttga atgaaaaaat
     5401 tttatgtaag tccaattttt cctattctag taggattgat tgcgtttgga gtcttatcca
     5461 ctttcattat ttttgttaat aataatctgt tgacggtttt aattttgttt ctttttgtag
     5521 gaggctatgt ttttttattt aagaaactga gagtgcatta tacaaggagt gatgtagaac
     5581 agatacagta tgtaaaccac caagcggaag aaagtttgac agctctattg gaacagatgc
     5641 ctgtaggtgt tatgaaattg aatttatctt ctggagaggt tgagtggttt aatccctatg
     5701 ctgaattgat tttgaccaag gaagatggtg attttgattt agaagctgtt caaacgatta
     5761 tcaaggcttc agtaggaaat ccgtctactt atgccaagct tggtgagaag cgttatgctg
     5821 ttcatatgga tgcttcttcc ggtgttttgt attttgtaga tgtatccagg gaacaagcca
     5881 taacagatga attggtaaca agtcgaccag tgattgggat tgtctctgtg gataattatg
     5941 atgatttgga ggatgcaact tctgagtcag atattagtca aatcaatagt tttgtagcta
     6001 attttatatc agagttttca gaaaaataca tgatgttttc tcgtcgggta agtatggatc
     6061 gattttatct atttactgac tacacggtgc ttgagggctt gatgaatgat aaattttctg
     6121 ttattgatgc tttcagagaa gagtcgaaac agagacagtt gcccttgacc ttaagtatgg
     6181 gattttctta tggcgatgga aatcatgatg agatagggaa agttgctttg ctcaatttga
     6241 acttggctga agtacgtggt ggcgaccagg tggttgttaa ggaaaacgac gaaacgaaaa
     6301 atccagttta ttttggtggt gggtctgctg cttcaatcaa gcgtacacgg actcgtacgc
     6361 gcgctatgat gacagctatt tcagataaga ttcggagtgt agatcatgtt tttgtagtcg
     6421 gtcacaaaaa tttagacatg gatgctttgg gctctgctgt aggtatgcag ttgttcgcca
     6481 gcaatgtgat tgaaaatagc tatgctcttt atgatgaaga acaaatgtct ccagatattg
     6541 aacgagctgt ttcattcata gaaaaagaag gagttacgaa gttgttgtct gttaaggatg
     6601 caatggggat ggtgaccaat cgttctttgt tgattcttgt agaccattca aagacagcct
     6661 taacattatc aaaagaattt tatgatttat ttacccaaac cattgttatt gaccaccata
     6721 gaagggatca ggattttcca gataatgcgg ttattactta tatcgaaagt ggtgcaagta
     6781 gtgccagtga gttggtaacg gaattgattc agttccagaa ttctaagaaa aatcgtttga
     6841 gtcgtatgca agcaagtgtc ttgatggctg gtatgatgtt ggatactaaa aatttcacct
     6901 cgcgagtaac tagtcggaca tttgatgttg ctagctatct cagaacgcgc ggaagtgata
     6961 gtattgctat ccaggaaatc gctgcgacag attttgaaga atatcgtgag gtcaatgaac
     7021 ttattttaca ggggcgtaaa ttaggttcag atgtactaat agcagaggct aaggacacga
     7081 aatgctatga tacagttgtt attagtaagg cagcagatgc catgttagcc atgtcaggta
     7141 ttgaagcgag ttttgttctt gcgaagaata cacaaggatt tatctctatc tcagctcgaa
     7201 gtcgtagtaa actgaatgta caacggatta tggaagagtt aggcggtgga ggccacttta
     7261 atttggcagc agctcaaatt aaagatgtaa ccttgtcaga agcaggtgaa aaactgacag
     7321 aaattgtatt aaatgaaata aaggaaaagg agaaagaaga atgaaagtaa tctttttagc
     7381 agatgttaaa ggaaaaggta aaaaaggcga aattaaggaa gtaccaacag ggtatgcgca
     7441 aaactttctt atcaaaaaga atctagccaa agaagcgact gctcaagctg taggtgaact
     7501 tcgtggtaaa caaaaatcgg aagaaaaagc tcacgctgag atgattgcag aagggaaagc
     7561 aattaaagca caacttgaag cagaagaaac tgttgtagaa tttgttgaaa aagttggtcc
     7621 agatggtcgt acctttggtt ctattaccaa taagaagatt gcagaagaat tacaaaagca
     7681 atttggaatt aagattgata aacgtcatat tcaagtacaa gctccgattc gagcggttgg
     7741 tttgattgat gtgccagtga aaatctatca agatatcaca agtgtaatca atcttcgtgt
     7801 gaaagaagga taagtttaca ccttcttgac aagattgtaa aaggaaggga agtctgatgg
     7861 cagaagtaga agagttacga gtacaacctc aagatatctt agctgagcaa tccgttttag
     7921 gggctatctt tattgatgag agtaaacttg tttttgtgcg agaatacatt gagtctcggg
     7981 acttttttaa gtatgcccat cgtttgattt tccaagccat ggtcgattta tccgatcgtg
     8041 gtgatgccat agatgcaaca acggttcgta ctatccttga taatcaaggt gatttacaga
     8101 atattggtgg cttgtcttac ttggttgaga ttgtcaattc tgtgccaact tctgctaatg
     8161 cggagtatta tgctaagatt gttgcagaaa aagcaatgct acgtcgttta attgccaagt
     8221 tgacagagtc tgtcaaccaa gcttacgaag cgtcacaacc agctgatgaa attattgctc
     8281 aggcagaaaa agggctgatt gatgtcagtg aaaatgcaaa tcgaagcggg tttaagaaca
     8341 ttcgagatgt gttgaatctc aactttggaa atctggaagc tcgctcgcaa caaacgaccg
     8401 atattacagg tattgcgaca ggttatcgtg atttggatca tatgacaaca ggacttcatg
     8461 aggaggagtt gattatctta gcagctcgtc cagcagttgg taagacagca tttgccttga
     8521 atatcgctca gaatattggg actaagttgg acaaaacggt tgctattttt tcactcgaaa
     8581 tgggtgcgga aagcttggta gaccgtatgt tagctgcaga aggcttggtg gagtcacatt
     8641 ctatccgtac agggcaattg acagatgagg agtggcaaaa atatactatt gctcagggta
     8701 atctagctaa cgccagtatc tatatcgatg atacgccagg tattcggatt acagagattc
     8761 gttctcgttc tcgtaaattg gctcaagaaa ctggaaatct tggtttgatt ttgatagact
     8821 atttgcaact tatcacggga actggtcgag aaaatcgtca acaagaagtt tctgaaattt
     8881 ctcgtcagtt gaaaatacta gccaaggaat tgaaggttcc agtaatcgct ctgagtcagc
     8941 tttctcgtgg tgtagaacaa cgtcaggaca agagaccggt cttgtctgat attcgtgaat
     9001 ctgggtctat tgagcaggac gctgatatcg tagcttttct ctatcgcgat gactactatg
     9061 aacgtggtgg tgaagaagag gagggtatcc caaataataa ggtggaagtt attatcgaga
     9121 aaaaccgtag tggagctcgt ggaacagtgg aattgatttt ccaaaaagaa tacaataaat
     9181 tttcaagtat ctcaaagagg gaggcataag atgtcagatg catttacaga tgtagccaag
     9241 atgaaaaaaa tcaaagaaga aatcaaggca catgagggac aagtcgtaga aatgactttg
     9301 gagaatggtc gtaagcgcca aaaaaataga ttgggtaagc taattgaagt ttatccatcc
     9361 ctatttattg tggagtttgg ggatgtggaa ggagataaac aagttaatgt ttacgttgaa
     9421 tcctttactt actcagatat tcttacagaa aagaatttga ttcattatct tgactaaagt
     9481 gagaaatttt ctcacttttt cttttttctc cgaataattt aggtgaaggc aatcatcgct
     9541 ttatattatt ttttaaggag gaagaatgaa aattttaccg tttatagcaa gaggaacaag
     9601 ttattacttg aagatgtcag ttaaaaagct tgttcctttt ttagtagtag gattgatgct
     9661 agcagctggt gatagtgtct atgcctattc cggaggaaat ggatcgattg cgcgtgggga
     9721 tgattatcct gcttattata aaaatgggag ccaggagatt gatcagtggc gcatgtattc
     9781 tcgtcagtgt acttcttttg tagcctttcg tttgagtaat gtcaatggtt ttgaaattcc
     9841 ggcagcttat ggaaatgcga atgaatgggg acatcgtgct cgtcgggaag gttatcgtgt
     9901 agataataca ccgacgattg gttccattac ttggtctact gcaggaactt atggtcatgt
     9961 tgcctgggtg tcaaatgtaa tgggagatca gattgagatt gaggaatata actatggtta
    10021 tacagaatcc tataataaac gaattataaa agcaaacacg atgacaggat ttattcattt
    10081 taaagatttg gatggtggca gtgttgggaa tagtcaatcc tcaacttcaa caggcggaac
    10141 tcattatttt aagaccaagt ctgctattaa aactgaaccc ctagttagtg caactgtgat
    10201 tgattactat tatcctggag agaaggttca ttatgatcaa attctcgaaa aagacggcta
    10261 caagtggttg agttatactg cctataatgg aagctatcgt tatgttcaat tggaggctgt
    10321 gaataaaaat cctctaggtc attctgttct ttcttcaaca ggtggaactc attattttaa
    10381 gaccaagtct gctatcaaaa ctgaacccct agttagtgca actgtgattg attactatta
    10441 tcctggggag aaggttcatt atgatcagat acttgaaaaa gacggctaca agtggttgag
    10501 ttatacggct tataacggaa gtcgtcgcta tatacagcta gagggagtga cttcttcaca
    10561 aaattatcag aatcaatcag gaaatatctc tagctatgga tccaataata gttcaactgt
    10621 cggttggaag aaaataaatg gtagttggta tcatttcaaa tcaaatggtt ctaaatcaac
    10681 aggatggctg aaagacggtt ctagctggta ttatttgaaa ttatctggtg aaatgcagac
    10741 aggatggtta aaggagaatg gctcgtggta ttatctgggt agttcagggg caatgaaaac
    10801 aggctggtac caggtctctg gtaagtggta ttattcttac tcttcaggcg ccttagctgt
    10861 taatacgacg gtggatggct acagagtaaa cagtgatgga gaacgagtat agaaaattgg
    10921 agtaggagaa atttcctgct cttttctgta agcagtttcc ttgacatttt tcttattttg
    10981 cgctatcata tatacatata atatatggga gtctgtgtac agtctgagag gaagtgttaa
    11041 acttcgaccg cacctgatct gggtaatgcc agcggaggga acgatactta gtctaatttt
    11101 gcaccttttc catgtatggt aaaggttttt ctttttttta aaaggaaaac gagaagagga
    11161 ggttcttatg aaagcaagca ttgccttgca agttttaccc ctagtacagg ggattgatcg
    11221 gatagctgtt attgatcagg tcattgctta tctgcaaact caagaagtgc cgatggtagt
    11281 gacaccattt gaaacggtct tggaagggga gtttgatgag cttatgcgca ttctaaaaga
    11341 agcgctggaa gtggcagggc aggaggcaga caatgtcttt gccaatgtca aaataaatgt
    11401 aggagagatt ttaagtattg atgagaaact tgagaagtat actgagacga cacattagtc
    11461 tattgggctt tctcggagtc ttgtcaatct ggcagttagc aggttttctt aaactgctcc
    11521 ccaagtttat cctgccgaca cctcttgaaa ttctccagcc ctttgttcgt gacagagaat
    11581 ttctctggca ccatagctgg gcgaccttga gagtggcttt actggggctg attttgggag
    11641 ttttgattgc ctgtcttatg gctgtactca tggatagttt gacttggctc aatgacctga
    11701 tttaccctat gatggtggtc attcagacca ttccgaccat tgccatagct cctatcctgg
    11761 tcttgtggct aggttatggg attttgccca agattgtctt gattatctta acgacaacct
    11821 ttcccatcat cgtcagcatt ttggacggtt ttaggcattg cgacaaggat atgctgactt
    11881 tgtttagtct gatgcgggcc aagccttggc aaatcctgtg gcattttaaa attccagtca
    11941 gcctgcccta cttttatgct ggtctgaggg tcagtgtctc ctacgctttt atcacaacag
    12001 tggtatctga gtggttggga ggctttgagg ggcttggtgt ttatatgatt cagtccaaga
    12061 aattgtttca gtatgatacc atgttcgcta ttattattct ggtatcgatt atcagccttt
    12121 tgggtatgaa gttggtcgat attagtgaaa aatatgttat taaatggaaa cgttcgtaga
    12181 attagaatgt ttctgaaaaa gaaaagagga aatcaaaatg aagaaaacat ggaaagtgtt
    12241 tttaacgctt gtaacagctc ttgtagctgt tgtgcttgtg gcctgtggtc aaggaactgc
    12301 ttctaaagac aacaaagagg cagaacttaa gaaggttgac tttatcctag actggacacc
    12361 aaataccaac cacacagggc tttatgttgc caaggaaaaa ggttatttca aagaagctgg
    12421 agtggatgtt gatttgaaat tgccaccaga agaaagttct tctgacttgg ttatcaacgg
    12481 aaaggcacca tttgcagtgt atttccaaga ctacatggct aagaaattgg aaaaaggagc
    12541 aggaatcact gccgttgcag ctattgttga acacaataca tcaggaatca tctctcgtaa
    12601 atctgataat gtaagcagtc caaaagactt ggttggtaag aaatatggga catggaatga
    12661 cccaactgaa cttgctatgt tgaaaacctt ggtagaatct caaggtggag actttgagaa
    12721 ggttgaaaaa gtaccaaata acgactccaa ctcaatcaca ccgattgcca atggcgtctt
    12781 tgatactgct tggatttact acggttggga tggtatcctt gctaaatctc aaggtgtaga
    12841 tgctaacttc atgtacttga aagactatgt caaggagttt gactactatt caccagttat
    12901 catcgcaaat aacgactatc tgaaagataa caaagaagaa gctcgcaaag tcatccaagc
    12961 catcaaaaaa ggctaccaat atgccatgga acatccagaa gaagctgcag atattctcat
    13021 caagaatgca cctgaactca aggaaaaacg tgactttgtc atcgaatctc aaaaatactt
    13081 gtcaaaagaa tacgcaagcg acaaggaaaa atggggtcaa tttgacgcag ctcgctggaa
    13141 tgctttctac aaatgggata aagaaaatgg tatccttaaa gaagacttga cagacaaagg
    13201 cttcaccaac gaatttgtga aataatgaca gaaattagac tagagcacgt cagttatgcc
    13261 tatggtcagg agaggatttt agaggatatc aacctacagg tgacttcagg cgaagtggtt
    13321 tccatcctag gcccaagtgg tgttggaaag accaccctct ttaatctaat cgctgggatt
    13381 ttagaagttc agtcagggag aattgtcctt gatggtgaag aaaatcccaa ggggcgcgtg
    13441 agttatatgt tgcaaaagga tctgctcttg gagcacaaga cggtgcttgg aaatatcatt
    13501 ctgcccctct tgattcaaaa ggtggataag gcagaagcta tttcccgagc ggataaaatt
    13561 cttgcgacct tccagctgac agctgtaaga gacaagtatc ctcatgaact tagcggtggg
    13621 atgcgccagc gtgtagcctt actccggacc tacctttttg ggcacaagct ctttctctta
    13681 gatgaggcct ttagcgcctt ggatgagatg acaaagatgg aactccacgc ttggtatctt
    13741 gagattcaca agcagttgca gctaacaacc ctgatcatca cgcatagtat tgaggaggcc
    13801 ctcaatctca gcgaccgcat ctatatcttg aaaaatcgcc ctgggcagat tgtttcagaa
    13861 attaaactag attggtctga agatgaggac aaggaagtcc aaaagattgc ctacaaacgt
    13921 caaatcttgg cagaattagg cttagataag tagaaaaata gggagttggt gaagattatc
    13981 ctttaccagc gccctttttc ttttaaaaat gagaaaattt cggtataata gtcaaacaag
    14041 gtcaaggttt aaagagagag gtgggtttgt tatgagattt aaaaatacat cggatcatat
    14101 tgaggcctac atcaaggcga ttttagatca atctggtatc gtggagttgc aacggagtca
    14161 gttggcagat acctttcagg ttgttcctag tcagattaac tacgtgatca agacacgctt
    14221 tacggaaagt agaggctact tggttgaaag taagcgtggt ggcggaggct acattcgtat
    14281 aggacggatt gagttttcta gtcatcatga aatgctccgg gagctgcttt actcgattgg
    14341 tgagcgagtc agtcaagaaa tttatgagga tattctccag cttttggttg agcaggaatt
    14401 gatgaccaag caggagatga atttgctaga atcagtagct ttggatcgcg ttttaggaga
    14461 agaagctcca gttgttcgag caaacatgct acgtcaggtc atacaagagg tagatagaaa
    14521 agggaagtaa gatgaactat tcaaaagcat tgaatgaatg tatcgaaagt gcctacatgg
    14581 ttgctggaca ttttggagct cgttatctag agtcgtggca cttgttgatt gccatgtcta
    14641 atcacagtta tagtgtagca ggggcaactt taaatgatta tccgtatgag atggaccgtt
    14701 tagaagaggt ggctttggaa ctgactgaaa cggactatag ccaggatgaa acctttacgg
    14761 aattgccgtt ctcccgtcgt ttgcaggttc tttttgacga agcagagtat gtagcgtcag
    14821 tggtccatgc tgaggtacta gggacagagc acgtcctcta tgcgattttg catgatggca
    14881 atgccttggc gactcgtatc ttggagaggg ctggtttttc ttatgaagac aagaaagatc
    14941 aggtcaagat tgctgctctt cgtcgaaatt tagaagaacg ggcaggctgg actcgtgaag
    15001 atctcaaggc tttacgccaa cgccatcgta cagtagctga caagcaaaat tctatggcca
    15061 atatgatggg catgccgcag actcctagtg gtggtctcga ggactatacg catgatttga
    15121 cagagcaagc gcgttctggc aagttagaac cagtcatcgg tcgggacaag gaaatctcac
    15181 gtatgattca aatcttgagc cgtaagacta agaacaaccc tgtcttggtt ggggatgctg
    15241 gtgtcgggaa aacagctctg gcgcttggtc ttgctcagcg tattgctagt ggtgacgtgc
    15301 ctgcggaaat ggctaagatg cgcgtgttag aacttgattt gatgaatgtc gttgcaggga
    15361 cacgcttccg tggtgacttt gaagaacgca tgaataatat catcaaggat attgaagaag
    15421 atggccaagt catcctcttt atcgatgaac ttcacaccat catgggttct ggtagcggga
    15481 ttgattcgac tctggatgcg gccaatatct tgaaaccagc cttggcgcgt ggaactttga
    15541 gaacggttgg tgccaccact caggaagaat atcaaaaaca tatcgaaaaa gatgcagcac
    15601 tttctcgtcg tttcgctaaa gtgacgattg aagaaccaag tgtggcagat agtatgacta
    15661 ttttacaagg tttgaaggcg acttatgaga aacatcaccg tgtacaaatc acagatgaag
    15721 cggttgaaac agcggttaag atggctcatc gttatttaac cagtcgtcac ttgccagact
    15781 ctgctatcga tctcttggat gaggcggcag caacagtgca aaataaatca aagcatgtaa
    15841 aagcagacga ttctgacttg agtccagctg acaaggcctt gatggatggc aagtggaaac
    15901 aggcagccca gctaatcgca aaagaagagg aagtgcctgt ctataaagac ttggtgacag
    15961 agtctgatat tttgaccacc ttgagtcgct tgtcaggtat ccccgttcaa aaactgactc
    16021 agactgatgc caagaaatac ttaaacttgg aagctgaact gcacaaacgt gtcatcggtc
    16081 aagaccaagc tgtttcaagt attagtcgtg ccattcgccg caatcagtca ggaattcgca
    16141 gtcacaagcg tccgatcggt tcctttatgt tcctaggacc cacaggtgtc ggtaagaccg
    16201 agttggccaa ggctttggca gaagttctct ttgatgacga atcagccctt atccgctttg
    16261 atatgagtga gtatatggag aaattcgcag ccagccgtct aaatggagct cctccaggct
    16321 atgtgggtta cgaagaaggt ggggagttga cagagaaggt tcgcaataaa ccctattccg
    16381 ttctcctctt tgatgaggta gagaaggccc atccagatat ctttaatgtt ctcttgcagg
    16441 ttctggatga cggtgtcttg acagatagca agggacgcaa ggtcgatttt tcaaatacca
    16501 ttatcattat gacatcgaac ttaggtgcga ctgcccttcg tgatgataag actgttggtt
    16561 ttggggctaa ggatattcgt tttgaccagg aaaatatgga aaaacgcatg tttgaagaac
    16621 tgaaaaaagc ttatagaccg gaattcatca accgtattga tgagaaggtg gtcttccata
    16681 gcctatctag tgaccatatg caggaagtgg tgaagattat ggtcaagcct ttagtggcaa
    16741 gtttggctga aaaaggcatt gacttgaaat tacaagcttc agctctgaaa ttgttagcaa
    16801 atcaaggata tgacccagag atgggagctc gcccacttcg cagaaccctg caaacagaag
    16861 tggaggacaa gttggcagaa cttcttctta agggagattt agtggcaggc agcacactta
    16921 agattggtgt caaagcaggc cagttaaaat ttgatattgc ataaaagaat aaaagtatca
    16981 gcatctgacc ataagtcaca gtggagtgaa actcaatgaa aatcaaagag caaactaggc
    17041 agctagccgc aggttgctca aaacactgtt ttgaggttgc agatagagct gacgtggttt
    17101 gaagagattt tcgaagagta tgaaactaaa acctatagct tctaaacgat ccgtggtttt
    17161 caccattcaa cacaaaattc atatgtttat taccctccgt cgtatttgtc ttagagcgtg
    17221 tgtagtagaa aaagagcagt cttatctgaa atttttattc tttcaaaaga gacctgtttc
    17281 ttttttgcat gtcaaatccg ttctagctgg tatttgaaaa atcaaactaa tattcaataa
    17341 aaatcaaaga acaaactagg aagctagccg caggttgctc aaaacactgt tttgaggttg
    17401 tagatagagc tgacgtagtt tgaagagatt ttcgaagagt ataagctgca agatgaatga
    17461 ttttcttgta ttgacgttgt tgttgacaaa aagtagcgga taaatgaaat ccattccatt
    17521 atcatagatg ataggctggt aggaaatttt caaatagcat acagaaaata gatgtatgga
    17581 gttctggtag tagaaaggga gagagatgaa cattttagtt gcagatgacg aggaaatgat
    17641 tagagaagga attgcagcat ttctgacaga agagggttat catgtcatta tggctaagga
    17701 tggacaagag gtcttggaaa aatttcaaga tctccctatc catctcatgg tactggattt
    17761 aatgatgcct aggaagagtg gttttgaagt gttaaaagaa atcaatcaaa agcacgatat
    17821 tcctgtcatc gtcttgagtg ctctgggaga tgaaactact cagtcacagg tatttgatct
    17881 ctatgctgat gatcatgtga caaaaccttt ttctttggta ctgcttgtca agcgtattaa
    17941 ggcgcttatc agacgttact acgtcataga ggatatttgg cgatatcagg atgtaacagt
    18001 ggattttacc tcttacaaag cacattataa aaatgaagaa attgatctca aaccaaagga
    18061 attactggta ctaaagtgtt tgattcagca taaaaatcaa gttttaagta gagagcagat
    18121 attggaagaa atttcaaaag atgtagctga tttaccttgt gatagggtcg ttgatgtcta
    18181 tattcgtact cttcgcaaaa aattagcttt agattgtatc gtgactgtga aaaatgttgg
    18241 gtataagatt agcttatgat aaaaaatcct aaattattaa ccaagtcttt tttaagaagt
    18301 tttgcaattc taggtggtgt tggtctagtt attcatatag ctatttattt gacctttcct
    18361 ttttattata ttcaactgga gggggaaaag tttaatgaga gcgcaagagt gtttacggag
    18421 tatttaaaga ctaagacatc tgatgaaatt ccaagcttac tccagtctta ttcaaagtcc
    18481 ttgaccatat ctgctcacct taaaagagat attgtagata agcgactccc tcttgtgcat
    18541 gacttggata ttaaagatgg aaagctatca aattatatcg tgatgttaga tatgtctgtt
    18601 agtacagcag atggtaaaca ggtaacagtg caatttgttc acggggtgga tgtctacaaa
    18661 gaagcaaaga atattttgct tttgtatctc ccatatacat ttttggttac aattgctttt
    18721 tcctttgttt tttcttattt ttatactaaa cgcttgctca atcctctttt ttacatttca
    18781 gaagtgacta gtaaaatgca agatttggat gacaatattc gttttgatga aagtaggaaa
    18841 gatgaagttg gtgaagttgg tgaagttgga aaacagatta atggtatgta tgagcacttg
    18901 ttgaaggtta ttcatgagtt ggaaagtcgt aatgagcaaa ttgtaaaatt gcaaaatcaa
    18961 aaggtttcct ttgtccgcgg agcatcacat gagttgaaaa cgcctttagc cagtcttaga
    19021 attatcctag agaatatgca gcataatatt ggagattaca aagatcatcc aaaatatatt
    19081 gcaaagagta taaataagat tgaccagatg agccacttat tggaagaagt actggagtct
    19141 tctaaattcc aagagtggac agagtgtcgt gagactttga ctgttaagcc agttttagta
    19201 gatattttat cacgttatca agaattagct cattcaatag gtgttacaat tgaaaatcaa
    19261 ttgacagatg ctaccagggt cgtcatgagt cttagggcat tggataaggt tttgacaaac
    19321 ctgattagta atgcaattaa atattcagat aaaaatgggc gtgtaatcat atccgagcaa
    19381 gatggttatc tctctatcaa aaatacatgt gcgcctctaa gtgaccaaga actagaacat
    19441 ttatttgata tattctatca ttctcaaatc gtgacagata aggatgaaag ttccggtttg
    19501 ggtctttaca ttgtgaataa tattttagaa agctatcaaa tggattatag ttttctccct
    19561 tatgaacacg gtatggaatt taagattagc ttgtagacag attagttttt tattaaagtt
    19621 catatagggt taacataagt gtgttattct ttgtgtagat aaaagaaagg atactaatat
    19681 ggtattagcg attattttag taacattctt tattcgattg atttttttaa agcgttcgat
    19741 agagaatgag aaacgaatcc ttagcaatgg cgggaaagaa tttggagttg agaatacaaa
    19801 acgattaact atggctcata ttgtttttta tctctcttgc ttggttgagg caatggtgca
    19861 caagacaatt tttgatggca tgggcatggt tggtttagtc ttgcttattt tttctatgct
    19921 gatgttgatg ttggtgattc acttgttggg agatatttgg acagtgaagc ttatgcttgt
    19981 caataatcac aaatatgtag atcatatctt gtttaggaca gtaaaacatc ctaattactt
    20041 tttaaatatt cttcctgagt tgattggctt gaccttgttg agtcatgctt atgtgacttt
    20101 tgttttagtt tttccagttt atgcagttat tttgtatcga cgaatagctg aagaggaaaa
    20161 gctattacat gaagttataa tcccaaatgg aagcataaag agataaatac aaaattcgat
    20221 ttatatacag ttcatattga agtgatatag taaggttaaa gaaaaaatat agaaggaaat
    20281 aattatgttt gcatcaaaaa gcgaaagaaa agtacattat tcaattcgta aatttagtat
    20341 tggagtagct agtgtagctg ttgccagtct ttttatggga agtgtggttc atgcgacaga
    20401 gaaagaggga agtacccaag cagccacttc ttctaatatg gcaaagacag aacataggaa
    20461 agctgctaaa caattcgatg aatatataaa caaaatgatc caattagata gaagaaaaga
    20521 tacccaaaat cgcaccttaa tcataaagtt gagcgcaatt aaaacgaagt atttgcgtga
    20581 attaaatgtt ttagaagaga agtcgaaaga tgagttgccg ccaaaaataa aagaagagtt
    20641 aaccgcagct tttgagcagt ttaaaaaaga tacattacca acagaaccag gaaaaaaggt
    20701 agcagaagct aagaagaagg ttgaagaagc tgagaagaag gtagcagaag ctaagaaaaa
    20761 agccaaggct caaaaagaag aagattaccg taactaccca accattactt acaaaacgct
    20821 tgaacttgaa attgctgagt tcgatgtgaa agttaaagaa gcggagcttg aactagtaaa
    20881 aaaggaagct gacgaatctc gaaacgaggg cacaattaac caagcaaaag cgaaagttga
    20941 gagtgaaaaa gctgaggcta caaggttaaa aaaaatcaag acagatcgtg aaaaagcaga
    21001 agaagaagct aaacgaagag cagatgctaa agagcaagat gaatcaaaga ggcgaaagag
    21061 tcgggtaaaa cgaggagatc ttggagagca agcaacacct gataaaaaag aaaatgatgc
    21121 gaagtcttca gattctagcg taggtgaaga aactcttcca agcccatccc tgaaaccagg
    21181 aaaaaaggta gcagaagctc agaagaaggt tgaagaagct aagaaaaaag ccaaggatca
    21241 aaaagaagaa gatcgccgta actacccaac caatacttac aaaacgcttg aacttgaaat
    21301 tgctgagtcc gatgtgaaag ttaaagaagc ggagcttgaa ctagtaaaag aggaagctaa
    21361 ggaatctcaa aacgaggaaa aaattaagca agcaaaagcg aaagttgaga gtaaaaaagc
    21421 tgaggctaca aggttagaaa acatcaagac agatcgtaaa aaagcagaag aagaagctaa
    21481 acgaaaagca gcagaagaag ataaagttaa agaaaaacca gctgaacaac cacaaccagc
    21541 gccggctcct caaccagaaa aaccagctcc aaaaccagag aagccagctg aacaaccaaa
    21601 agcagaaaaa acagatgatc aacaagctga agaagaccat gctcgtagat cagaagaaga
    21661 gtataatcgc ttgactcaac agcaaccgcc aaaaactgaa aaaccagcac aaccatctac
    21721 tccaaaaaca ggctggaaac aagaaaacgg tatgtggtac ttctacaata ctgatggttc
    21781 aatggcgaca ggatggctcc aaaacaatgg ctcatggtac tacctcaaca gcaatggcgc
    21841 tatggcgaca ggatggctcc aaaacaatgg ttcatggtac tatctaaacg ctaatggttc
    21901 aatggcaaca ggatggctcc aa
//
LOCUS       DAVKJD010000027        21687 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKJD010000027 DAVKJD010000000
VERSION     DAVKJD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.027"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(49..855)
                     /gene="yidA"
                     /locus_tag="VW157_001052"
     CDS             complement(49..855)
                     /gene="yidA"
                     /locus_tag="VW157_001052"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9552367.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(868..2172)
                     /locus_tag="VW157_001053"
     CDS             complement(868..2172)
                     /locus_tag="VW157_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9552368.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2244..2621
                     /locus_tag="VW157_001054"
     CDS             2244..2621
                     /locus_tag="VW157_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9552369.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2712..3044
                     /locus_tag="VW157_001055"
     CDS             2712..3044
                     /locus_tag="VW157_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9552370.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            3056..4627
                     /gene="ffh"
                     /locus_tag="VW157_001056"
     CDS             3056..4627
                     /gene="ffh"
                     /locus_tag="VW157_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9552371.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(4822..6105)
                     /locus_tag="VW157_001057"
     CDS             complement(4822..6105)
                     /locus_tag="VW157_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9552372.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6408..7121)
                     /gene="rsmG"
                     /locus_tag="VW157_001058"
     CDS             complement(6408..7121)
                     /gene="rsmG"
                     /locus_tag="VW157_001058"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9552373.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHLYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7215..7775
                     /locus_tag="VW157_001059"
     CDS             7215..7775
                     /locus_tag="VW157_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9552374.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            7777..8676
                     /gene="htpX"
                     /locus_tag="VW157_001060"
     CDS             7777..8676
                     /gene="htpX"
                     /locus_tag="VW157_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9552375.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINAPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8720..10279)
                     /locus_tag="VW157_001061"
     CDS             complement(8720..10279)
                     /locus_tag="VW157_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9552376.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQKIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYESYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSSIYRLTE
                     HGLELVNLEDV"
     gene            complement(10518..10835)
                     /locus_tag="VW157_001062"
     CDS             complement(10518..10835)
                     /locus_tag="VW157_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9552377.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FGF"
     gene            11005..11547
                     /locus_tag="VW157_001063"
     CDS             11005..11547
                     /locus_tag="VW157_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9552378.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11547..12176
                     /gene="nth"
                     /locus_tag="VW157_001064"
     CDS             11547..12176
                     /gene="nth"
                     /locus_tag="VW157_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9552379.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12387..12908
                     /gene="pyrR"
                     /locus_tag="VW157_001065"
     CDS             12387..12908
                     /gene="pyrR"
                     /locus_tag="VW157_001065"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9552380.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            12927..13850
                     /locus_tag="VW157_001066"
     CDS             12927..13850
                     /locus_tag="VW157_001066"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9552381.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            13900..14979
                     /gene="carA"
                     /locus_tag="VW157_001067"
     CDS             13900..14979
                     /gene="carA"
                     /locus_tag="VW157_001067"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9552382.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15292..18468
                     /gene="carB"
                     /locus_tag="VW157_001068"
     CDS             15292..18468
                     /gene="carB"
                     /locus_tag="VW157_001068"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9552383.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLTTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     regulatory      complement(18684..18772)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18981..19610)
                     /locus_tag="VW157_001069"
     CDS             complement(18981..19610)
                     /locus_tag="VW157_001069"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9552384.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(19612..20394)
                     /gene="thiM"
                     /locus_tag="VW157_001070"
     CDS             complement(19612..20394)
                     /gene="thiM"
                     /locus_tag="VW157_001070"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9552385.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVIASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(20637..21305)
                     /locus_tag="VW157_001071"
     CDS             complement(20637..21305)
                     /locus_tag="VW157_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9552386.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(21520..21608)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         6348 a         4263 c         4621 g         6455 t
ORIGIN      
        1 attcattgga ttttattgct aatcaattct tattgaaaaa tgatactttt acagtaccca
       61 tgtacgaata gcatgggcaa cgccggattc gtcatttgtt ttggtgatgt atttggcgat
      121 ttttttgatt tctggatttc cgttttccat gactacaggg ttgccaacga cttccagcat
      181 ggcacggtca ttttcttcgt ctccgatagc catggtttca tctttggtta atcctagttt
      241 ttcagctaag tgagtaatag ctgaaccctt gtctacattc tttttaagga gttcgaggta
      301 gaaaggagca gatttgttga tagagtagtg ctcgtaaaat tcggctggta ttttttcaat
      361 cgcagcatcg aggatttctg gttcatcgat gaacatacac ttgacaattt ccttgccagc
      421 catttcttca ggggtacggt agaagatagg catgctgacg agggttgatt cgtgtacagt
      481 gtattttccg atattgcgat ttgcagtata gataccgtcc ttggtaatgg catgcatgtg
      541 gacaccgagc ttgcgactga ggaattccat atccagataa tcctcatagg tcaaggattc
      601 gctgataatc tcatggccag tagcagtttc ttggacaagg gcaccattga aggtcaccac
      661 atagtcacct tggtctctca actgcaagtc gtccagaagt ttggcaacac ctgcgatggg
      721 gcggccagtt gcaatcacga ctttgacacc agcttctttg gcatcttgga tggcagaaaa
      781 gacttcagga gtgatttcct tttggctgtt gactagggtt ccgtcaatat cgacggcgat
      841 tagtttaata ctcataaatt tcctctttta gtttttattt ggggtaaaat gatcgttctc
      901 aatcaagtgt aaaaattgct gggtgatgct tgcgaagatg ctgttttggt ctaacatttc
      961 tttcgggaaa taaaagcgat tatctccgtg gcgacttcca gcaagggatt ggacgatagg
     1021 ggacaggcta gagagttctg ccagttctcc atttttttgt aaaatctcaa tctgtgtcct
     1081 tggattttca gattcgggac ggtaaatatc ataagggagg tcaaagttct tatgaatggc
     1141 agtatagtag tcgggatcaa agccgatgtc ctcaaccaat tttctcatgc tagcgagttg
     1201 gtcttggtct tcttgtgaaa aggtaatgga tttaaagacc ttgcggttga caaagcgatg
     1261 cgataaatct gcaagaatct tgtcaggact ggtcatccag agttggaagt aggtattcat
     1321 cacaccatca tccagagcca gatagtcagt caaggtcaca tttttttcga agaaaggcag
     1381 gaggtgtgga gaagttcggg caaagaaatc cttgtcctca ggatagagtt ccttggcgcg
     1441 tttgagaaga ttctgtagga gaacttccat ggcgcgtgtt gcggggtgga aataaacctg
     1501 catgtacatc tggtagcgac tgaggacgta gtcttcgatg gcgtgcatgc cattgcgctg
     1561 aaaggcgata ccattttcga taggacgaat gactcggagg attcgagtca ggtcaaattc
     1621 cccataggat gctcctgtaa aataggagtc gcgcaagaga tagtccatgc gatctgcgtc
     1681 aatctgacta gaaatgagct gcacgacctg cttgttagga taggtatggt ctatgacact
     1741 ggcaactttc tctggaaaat cgggcgctac ttgtagcaag acttggtgaa tctctgtctc
     1801 aggattttga ataatctcct gagtaatggc ttcatggtct gtatcaaaga gattttcaaa
     1861 agtatgggag taggcaccat gcccaaggtc gtgtaggaga gcagcggtca tggtcaagag
     1921 agactcggca ggattccatt cctcaggata tttttcttcg aaaatctctg tgatgcgtcg
     1981 tgcaatttca tagactccta gacagtgaga gaagcgactg tgttctccac cgtggaaggt
     2041 ataactggaa gttcccagtt gtttgatccg gcgcaaacgc tgaaattctt ttgtattaat
     2101 caagtcatag atgatttgat tattgacatg gatgtagttg tgaacagggt cacggaatac
     2161 tttttcgttc atatgaccat tatagcaaaa tcctgctctt tttggtaaaa tagtaggaca
     2221 agagacaaga aagagggcct gatgtgaaga ttcggatgcg aaatacaatt cagtttgatg
     2281 agcagttgga agtgattgac cagctttatg atgtggaagt gcatgaaaaa ggagattata
     2341 gctacctgct tttctataat gaggaaaagg aaaaagtagt gattaaattt catggtcaag
     2401 aacttatgat gagccgattt tctaatccca agaccattat gcgctttcta aaggatagcg
     2461 atagtttagc ctatattcct acacctatgg gcatgcagga gtttatcatc caaacgagcc
     2521 attatcaagt tgataggcaa aagattgaac tagattatca actacaaaat caagagggac
     2581 atccttttgc cagctatcaa ttggaaatta cttggggcta ggctcatgtc tttttcaaat
     2641 ttagctatct atcttcttgg atcggcttcc tagtgtggta gattaggcta atttttggta
     2701 taataaaagt tatggaaatc gaaaaaacca atcgtatgaa tgcgctcttt gaattttatg
     2761 cggcgctttt gacagataag caaatgaatt atatcgagct ctactacgct gatgattaca
     2821 gccttgctga aattgccgag gagttcggtg tcagtcgtca ggctgtctat gacaatatca
     2881 agcgaacaga aaagattctg gaagattatg agatgaaatt gcacatgtac tcggattata
     2941 ttgtccgcag tcagattttt gatcagattt tggagcgcta tcccaaggat gactttctgc
     3001 aggagcagat agaaatttta acaagcattg ataatagaga ataagaggaa gaaaaatggc
     3061 atttgaaagt ttaacagaac gtttgcagaa cgtctttaaa aatctacgta aaaaaggaaa
     3121 aatctctgaa tctgatgtcc aagaggcaac caaagaaatt cgcttggcct tgctcgaggc
     3181 cgacgttgcc ttgcctgttg taaaggactt tatcaagaaa gttcgtgagc gtgcagtcgg
     3241 gcatgaggtc attgatacac ttaatcctgc gcaacagatt attaaaatcg ttgatgagga
     3301 actgacagcc gttttaggtt ctgatacggc agaaattatc aagtcaccta agattccaac
     3361 catcatcatg atggttggtt tacaaggggc tggtaaaaca acctttgctg gtaaattggc
     3421 caacaaactc aagaaagaag aaaatgctcg tcctttgatg attgcggcgg atatttatcg
     3481 tccagctgcc attgaccagc ttaagacctt gggacaacag attgatgtgc ctgtctttgc
     3541 acttggaaca gaagtaccag ctgttgagat tgtacgtcaa ggtttggagc aagcccaaac
     3601 taatcataac gactatgtct tgattgatac tgcgggtcgt ttgcagattg atgagctcct
     3661 catgaatgag cttcgtgatg tgaaagcatt ggctcaacca aatgaaatct tgcttgtcgt
     3721 tgatgctatg attggtcagg aagcagccaa tgttgcgcgt gagtttaatg ctcagttgga
     3781 agtgactggg gtcatcctta ccaagattga tggcgatact cgtggtggtg ctgctctgtc
     3841 tgttcgtcac attactggaa aaccaatcaa gttcactggt acaggtgaaa agattacgga
     3901 cattgaaacc ttccacccag accgcatgtc tagccgtatc cttggtatgg gggatatgct
     3961 cactttgatt gagaaagctt ctcaggaata cgatgaacaa aaagcccttg aaatggctga
     4021 gaagatgcgc gaaaacacct ttgattttaa tgatttcatc gatcaattag atcaggtgca
     4081 aaatatgggg ccgatggaag acttgctcaa gatgattcca ggtatggcca acaatccagc
     4141 ccttcaaaac atgaaggtgg atgaacgcca gattgctcgt aaacgtgcga ttgtgtcttc
     4201 gatgacacct gaagaacgtg aaaacccaga tttgttaaat ccaagccgtc gccgtcgtat
     4261 tgctgctggt tctggaaata cattcgtcga agtcaataaa ttcatcaagg actttaacca
     4321 ggctaaacag ctcatgcagg gtgttatgtc tggggatatg aataaaatga tgaagcaaat
     4381 ggggattaat ccaaataacc ttcctaaaaa tatgccaaat atgggaggaa tggatatgtc
     4441 tgcccttgaa ggaatgatgg gacaaggcgg tatgcctgac ttatcagctc tcggaggagc
     4501 aggaatgcca gatatgagcc agatgtttgg tggcggtttg aaaggtaaaa ttggtgaatt
     4561 tgccatgaaa cagtccatga aacgtatggc taacaaaatg aagaaagcga agaagaaacg
     4621 caagtaagac aagagattga tgcaaatctc atttgatgag aaaataaagt aaaaatttct
     4681 atgataaaac gtacagtatc aaggttttag aatatctgat attatacgtt ttttgatttt
     4741 aataattttg ttaagacact tttattatgg tatgcgaaaa atccactcag ctgtctgagt
     4801 ggattaggtg tatttagact cttagtcttt attttcgtat ggcaagatca agttcaagat
     4861 gattcctgtc atggctgaaa gggcagtacc tgaaagtgta actggaccaa gtttaaggat
     4921 agctcctcca agtccaagaa ccaacatagc acttgcgatg atgaggtttc gcatttgagc
     4981 gaaatcaaca cgttctttaa tcaagacttt caaaccattg ctggcgataa ccccatagag
     5041 aaggattgac ataccaccaa gtacagcgtt tggaatagtt gaaatcaagg cagtgaattt
     5101 accaaggaag ctgagggcaa tcgcgatgaa ggcagcgtta cggataactg agacagaagc
     5161 gatacgagtc ataccgataa cccctgtatt ttctccgtaa gttgtattgg ctggtccacc
     5221 aaggaaggca gaaacagaag ttgcgatacc gtcaccaaga agagtacggt gaagacctgg
     5281 ttcttttaag aattgacggc cacagatttg acccaaaaca gtatggtctc cgatatgttc
     5341 agaaattgtt acgatagcga ttggcaagat agcgatggct tctggaccaa agtaaagatt
     5401 gtactcttta aaggcaccac ctgtgctaaa tggcaagtag aaaccaggaa tttcaaacca
     5461 gttagcttct aggacaggcg taaagtcaac caaaccaaga gttagtgcga agaggtaacc
     5521 accgataatg gcaaagagga atggaatgat tcgtaggaag ccttttcctt ttgtattgat
     5581 aaaggcagca attaggaaag taacaacggc taccagagca tttttccaat ttccgtctgc
     5641 tacaagacct gcattggtaa cagctgaacc tgcaagtcca agaccgataa cgatgatcat
     5701 aggaccgata atgattggtg gcaagagttt atcaatccat tttgttccta caaatcggat
     5761 gctggtagca acaaggacat agaccaaacc agtcaagata acccctgttt gagcagcaga
     5821 tacatccccc cccatttctt tcatagccag tgacatagct gtgataaagg cgaatgaaga
     5881 acctagataa actggaactt taaaaccagt agcaatcatg tagatgagtg ttccaacacc
     5941 tgaagcaaaa agggcaacag atacaggcat tcccaaaatc aatggtacca agatggtcgc
     6001 accaaacatg gcgaaaacgt gttggaaact aaggagaatt ccttttccag ccgaaggacg
     6061 ttggtcaacg tctaataaca aatcaacagt tgattcttgt ttcatataaa cctcactttt
     6121 gggcatcaga aaagagccca ttattttaca gcaataggcc ctcagagtaa gacttctcta
     6181 aaatctagac tttaaagagt ttcaaatatt tttgcgtctt cgtcacctca cgggatgaca
     6241 ttaaaaacct ttgcttagga tagtatagca gaaaaaaatg attttgtaaa tcattttttc
     6301 ccgagcctag aaataaaaga gcgaggctga ttttgtaaat cattttttcc cgagcctaga
     6361 aataaaagag cgaggctgat tttgtaaaca tttgtttact aaaaaattta aagtgggcgt
     6421 ttatttggca taccagcctt acgcggatat ttatttggtg tttctttttt cttttctacc
     6481 actgtgatgt aacgcggatc tctatttggt agggcgtagc tgagattgtc ttcgacctta
     6541 ctaaaaagga gattgagggc attcttagct tctaataatt cctcaggcgc attgctggcc
     6601 ttgagtgcca atagttttcc accaacctta aggtagggaa tggtcaattc agataggacc
     6661 tgcatacggg caaccgcacg agctgttaca aaatcatatt gagcacggaa gttcttgtct
     6721 tgggcaaaat cttcggcacg tccgtggtat aaatgaactc cgttcaaatc cagttcttga
     6781 gccaagagtt gtaggaagtt gatgcgctta ttgagtgaat caataatggt cacatctaac
     6841 tccggataga gaattttcat tggtagacta ggaaatcctg ccccagcccc gatatcaaga
     6901 agtttgatag tttcattggg aatcaaacct tgaagaatgg gtgcaatcga atcgtaaaaa
     6961 tgtttgagat aaacttcttc cttgtccgta atcgccgtca aattaatctt ctcattccac
     7021 tcgaccaaga gctcaaaata acgttcaaat tgttcttttt gctggtccga aagtggaaga
     7081 ttctgctcgg caagcaagtt gtaaaatgtt tttggtttca tagttatttc cttctttagt
     7141 atcatcttat tttaccacaa tcatagaaaa tttgatatac tggtactaat ctaaaagcag
     7201 aaaggactta tattatgact tggattattc ttggagttat cgctcttatt gttatttttg
     7261 tgattgttag ctataacggt ttggttaaaa atcgtatgca aaccaaggag gcttggagtc
     7321 agattgatgt tcagttgaaa cgtcgaaatg acctcttgcc aaacttgatt gagactgtaa
     7381 aaggttatgc caaatatgaa ggttctaccc ttgaaaaggt ggcagaacta cgtaaccaag
     7441 tggcggcagc gacttcacca gcagaagcta tgaaagccag tgatgccctc actcgtcagg
     7501 tttcaggtat ttttgcagtt gcagaaagct atccagattt gaaagctagt gctaactttg
     7561 ttaaattgca agaggagttg acaaacacag aaaataaaat ttcttactct cgtcaactct
     7621 ataacagtgt tgtcagcaac tacaatgtaa aattagaaac tttcccgagc aatattatcg
     7681 ctggaatgtt tggatttaaa gcggcagatt tccttcaaac acctgaagag gaaaagtcgg
     7741 ttcctaaagt tgattttagc ggtttaggtg actaagatgt tgtttgatca aattgcaagc
     7801 aataaacgaa aaacctggat tttgttgctg gtatttttcc tactcttagc tcttgttggt
     7861 tatgcggttg gttatctctt tataagatct ggacttggtg gtttggttat cgcactgatt
     7921 atcggcttta tctacgcttt gtctatgatt tttcaatcga cagagattgt catgtccatg
     7981 aatggagcgc gtgaggtgga tgagcaaacg gcaccagacc tctaccatgt agtggaagat
     8041 atggctctgg tcgcacagat tcctatgccc cgtgttttca tcattgatga tccagcctta
     8101 aatgcctttg cgacaggttc taatcctcaa aatgcggctg ttgctgcgac ttcaggtcta
     8161 ctagctatca tgaatcgtga agaactagaa gctgttatgg gacatgaagt cagtcatatt
     8221 cgtaattatg atatccgtat ttcgactatt gcagttgccc ttgctagtgc tatcaccatg
     8281 ctttctagta tggcaggtcg tatgatgtgg tggggtggag caggtcgcag acgaagtgat
     8341 gatgaccgag atggaaatgg tcttgaaatc attatgctag tggtttccct actagctatt
     8401 gtactggcac ctctcgctgc aaccttggtt cagctcgcta tttctcgtca gagggaattt
     8461 ctggcagatg catctagtgt cgagctgact cgcaatcccc agggaatgat taatgcccta
     8521 gataagttgg acaatagcaa acctatgagt cgccacgtcg atgatgctag cagtgccctt
     8581 tatatcaatg ctcccaagaa aggtgggggg ttccaaaaac tcttttatac ccacccacct
     8641 atctcagaac ggattgaacg tttaaaacag atgtaaaaga atccagaggt cgtctctgga
     8701 tttttgctat gttgcaaatt tatacatctt ctaaattaac taactccaaa ccgtgttctg
     8761 tcaagcggta gatgcttgaa cagacctctt ttaagaaacg acggtcatgc gaaacagtga
     8821 tgagaccgcc tggataggtt gcaaagagtt ttctgatttg gggttgagaa gtgggagaaa
     8881 agtttcgtgt gggttcatcc agcaggagaa agtttggttt gcgcaggact aaatccaaaa
     8941 gcaggagttt tccctgttgt ccgccagata aggagcgaat ttgatgctgc atttctggat
     9001 aactgaaatt gagactagct aggtgagatt ggattttctg tagttcctct ttttccccag
     9061 ttttgctgag gtaggctatt ggtgataaat ccaattgcag ttttttgtgg taatcttgtg
     9121 gcataaaacc aagggaaatc tctcttttat cattcagaag ttgctttaat ttggctaaca
     9181 gagttgattt cccaacacca tttggcccga taataccgat tttttcttgc ccacggacag
     9241 ttagttgtag tttttgaacc aaaactcggt cgtcaatgga caaattttct ttttccagtt
     9301 ggactaagac tttagaagct ggtaatggtt ggatgtctga aaagaaaagt tggatttgtt
     9361 cctcttcaag tggcttttga gtcatggact gagctgcctt ttcgtagcgt ttttcttgtg
     9421 agaggacagt tttcatcttt ttagccaata ggcgaccggc agtactgtct ttggtagctc
     9481 gaagcgcagt ttctacattt tgtttgactc tccgatgttt ttccatggtt ttatcgtagg
     9541 ctctttggtt gttagcagct tgctgacttt gtttggcaaa attagccttt ctctgctcac
     9601 tatagctttc atagtctaaa tgctctacta gcgtttccgc ttctttacgg tgtttgacca
     9661 gtcgcaagtg aacaatagtg tctgccgttt cagaaagaaa gtcttcatca tgggaaatga
     9721 aaataacggt ttgcctggtc ttttgaatct ggccttttag ccaatcaact gtctcaaggt
     9781 ctaggtcatt tgaaggttca tctaaaaata gaatctcaaa gggtttggct aactcatgga
     9841 taagctgaat tttcaaagct tctccccctg atagattgcc aatcttttgg tcacttgcga
     9901 aacgattgct atcaaaatgc aattcctccg ccaaacgata gaggatactg tagtctaaat
     9961 caatagaatc taaaaagaag tagtcgtgta aagttttctt tttcagctcc tcgggaagtt
    10021 tttgaggaat gtaggccagt gactgatagt cagattggat gtttcccttg atagtgaaat
    10081 cagacaaagc ttcccccatt aaaattttaa gtaaggttga tttgccattt ccttcttcac
    10141 caataatagc aaccttttcc ccgtcttgga tggtcatgct taggtcagat acaagatctc
    10201 gtaaatcttt gttttgtgtg atggttagat gattgatttt tatcatatga tttccctttc
    10261 tagaatgttc atagtgcata acaaaaagct ctactttacc tagtagagcc caaagtctat
    10321 gtcaaaaaaa ctctattaac atgccagaaa aagagcataa aagagcacac aaaaagagac
    10381 aaaaacaaga tctactaaat aaaggttctt tatttgatag acttagttgt tcatgtctcc
    10441 cttacctccg agtattagta tctttatatt atacctttaa ggaaattttg tcaacagaaa
    10501 actggaaatt ctatgctcta aaatccaaaa acaggtccat agagacttag tacgtgtttg
    10561 acatgctcgt aatggaattt atcatagttt cgccaagcag tgcgtgcttg atcgtttagt
    10621 tttaggctac ccagtccaat cagaagactg gtgggttggt aaaatttctc aagatcaatt
    10681 aagatactgt tgttcaactt ttccgttttt ttgagctctt gcatagtagt gtttagcagc
    10741 tcttgtagtc gaatatcatc tgccgtaccc tgtttacagt tttggtagct tttatttaac
    10801 ttggatagga gttgaatgag tttcttgtct tccatgtgag catcctcctt gatctgatgt
    10861 actctttccc tatagtatag cactaaaaaa caaaactgtc atagtaaata tgttaaaatt
    10921 ttgaatttta attacaaagt ttggtgtgct gatttgtagc cttttcatgg tataatcaag
    10981 agagtaaatc tttatggagg aagtatgaaa ttaaatattc aagaaattcg taagcagtct
    11041 gaaggtttga actttgaaca aacgttagat ttagttgatg acctgcgtgc acgtaatcaa
    11101 gaaattttag atgtaaaaga tatccttgca gttgggaaag tacaatatga agaccgtatg
    11161 tatttcttag attatcaact atcttatacc attgttcttg cttcgagtcg cagtatggag
    11221 ccagttgagt tagttgaatc ttatccagtc acggaagttt tcatggaagg tgcaactaac
    11281 catctaaatc aagaaatttt agatgatgac ttggtcttgc ccatcgaaaa tgaggcgctt
    11341 gaccttgctg agagtgtatc agacaatatc ttgctaaaca ttcctatcaa ggtcttgacg
    11401 gctgaagaag aagctggtca aggatttgtc tcaggaaatg actggcaaat catgacagag
    11461 gaagaatacc aagctcaaca agcagtaaag aaagaagaaa acagtccttt tgctggctta
    11521 caaggactat ttgacggaga tgaataatgg tcttgtcaaa aaaacgagca cgaaaggtgc
    11581 tagaagaaat cattgccctc ttcccagatg ccaagcctag tcttgatttt accaatcatt
    11641 ttgaactcct ggttgcggtc atgttatcag cccagacgac agatgcagcg gtaaataagg
    11701 ccacaccagg tctctttgtt gcctttccaa caccccaagc tatgtctgta gcgacagaga
    11761 gtgagattgc ttcacatatt tctcgcttgg gattgtatcg gaataaagct aaattcctta
    11821 aaaaatgtgc ccaacagtta ctagacgatt ttgatggtca agtccctcag acacgtgaag
    11881 aattggagag tttggcaggt gttggtcgca agacagccaa tgttgtcatg agtgtaggat
    11941 ttgggattcc agcctttgca gtggatactc atgtggagcg tatttgcaaa caccacgata
    12001 ttgtcaaaaa atcagcgacg ccacttgagg tggaaaagcg ggtcatggat atcttgccgc
    12061 ctgagcagtg gttagctgcc catcaggcca tgatttattt tggaagagcc atttgccatc
    12121 caaaaaatcc agagtgtgac cagtacccac aattatatga ttttagcaat ttgtaagatg
    12181 ctacattgaa actttttgct tgattttaag catgctttgt gctatagtaa atgataacta
    12241 aatattcctt taaacctgtc ccgtgaggca ggcaaggagc gcgataaagt agaagggtga
    12301 agtctatact gtgtgcagta gggctcgctt ggctataaat ttcaagtctc cttgtttaag
    12361 gagacttttc tttttggagg tttgtcatga aaacaaaaga agttgtagac gaattgaccg
    12421 tcaaacgagc gattacgcgt attacttatg agattatcga acgcaacaaa gatttgaata
    12481 agatcgtctt ggctggtatt aaaactcgtg gtgtctttat tgcccaccga atccaagaac
    12541 gtttgaagca gttagaaaat ctttcagttc ctgttgtgga attggatact aaacctttcc
    12601 gtgatgatgt taaaagtgga gaagatactt ctttggtttc tgtcgatgtg acagaccgcg
    12661 aagttatctt ggtggatgat gtgctctata caggtcgtac catccgtgct gctattgata
    12721 atattgtcgg tcatggtcgt cctgcgcgtg tgagtttagc agttctagtc gatcgtggac
    12781 atagagaatt gccaatccgt ccagattacg ttggaaaaaa tatcccaacc agtcgttctg
    12841 aagaaatcat cgtagagatg acagaacttg atgaccaaga cagagttttg attactgaag
    12901 aagcttagaa agttaaagga gtagccatgt cagaaaatca acaagcattg aaccatgtgg
    12961 tgtccatgga agacctcact gtcgatcaag taatgaaatt gatcaagcga ggaattgagt
    13021 ttaaaaatgg agcccagctt ccctatgaag accatccgat tgtttccaat ctcttctttg
    13081 aggattctac acggacacat aagtcctttg aagtcgcaga gattaaactt ggattggaac
    13141 gacttgactt tgatgtgaag actagttcgg ttaataaggg tgagacactt tatgacacca
    13201 ttttgactct gtctgcttta ggagtggatg tctgtgtgat tcgccatcca gaggtcgact
    13261 actatagaga attgattgct agtccaacga ttacgacttc catcatcaat ggtggagatg
    13321 gttcgggcca acaccctagc cagagcttgc ttgatttgat gaccatttat gaggaatttg
    13381 gccactttga gggtcttaaa gttgctattg caggtgactt ggaccattca cgcgttgcca
    13441 aatccaatat gcagattttg aaacgcttgg gatctgaact ctttttcgct ggacctgagg
    13501 aatggagaag tcaagagttt gcagactatg gacagtttgt aaccattgat gaaatcattg
    13561 atcaggttga tgtcatgatg tttctccgtg tgcaacacga acgccatgat agtggagctg
    13621 tattttcaaa agaagactac catgcccaac atggcttgac tcaagaacgt tacgatcgtt
    13681 tgaaagaaac agcaatcctc atgcacccag ctccaatcaa tcgtgatgta gaaatagcag
    13741 accacttggt tgaagcacca aaatcacgga ttgtccaaca aatgaccaat ggtgtctttg
    13801 ttcgaatggc aatcttagaa tccgtactag cgagtagaaa cgccaactaa aacacaagac
    13861 gttaaaagac gccagtgagc gtccacgaga ggtgaaaata tgacaaaaag aattctagta
    13921 ttagaagatg gcacagtttt tgaaggcaag gccttcggag cagatattga tgtaacaggc
    13981 gaaatcgtct ttaatacagg gatgaccggc taccaagaat ccattacaga ccagtcttat
    14041 aatggacaaa tcttgacctt tacttatcct ttggtaggaa attatggtat taatcgtgat
    14101 gattatgaat ccattattcc aacttgtaag ggagtcgttg tttttgaaga agcgcgtaga
    14161 gctagcaact ggcgcaacca aatgaccttg gatgaatttt tgaaagccaa gaaaattcca
    14221 gggatttcag gaattgatac gcgtgctctt accaagatta tccgtaagca tggtactatg
    14281 cgtgcaacct tgacccatgt tggggacagt atggaccatg tgacggacca gctccaagca
    14341 acagttttac cgacagacaa tatcaaacag gtttctacta aaacgtctta tccagctcca
    14401 ggagttggtt tgagcgtggt gctagtggac tttggtctca agcactcaat cctacgtgaa
    14461 ctttctaaac gcaactgtaa cgtgacagtt gttccttatt cgacaacggc tgaagaaatt
    14521 ctccatctca atcctgatgg agttatgttg tcaaatggtc caggtaaccc agaagacgtt
    14581 ccacaagcac tcgacatgat tcgcggtgta caaggaaaaa ttccaatctt tggtatttgt
    14641 atgggacacc aactctttgc aatggcaaac ggggctaaga cctataagat gaagtttggc
    14701 caccgtggat tcaaccatgc ggtacgtgaa atcgcaacag gacgagtaga cttcaccagc
    14761 cagaaccatg gttatgcagt cagccgcgag gatttgccag agcacttgat tattacccac
    14821 gaagaaatca atgacaagtc agtggaaggt gtgcgccaca gataccaacc tgctttctct
    14881 gttcaatacc acccagatgc agctcctggg ccacacgacg ctagctacct atttgacgag
    14941 tttatcgaga tgatggaagt ttttaaacaa tcaaactaag aacgagtaaa agctcgtcgg
    15001 agaatttatg taactgaaca cggacggaaa gctcagaatg tagagaaacc aacttggatt
    15061 gtcaatcctt ccttgatttt ctaaaattca atcgctttct attcgtcctt tgtatcttat
    15121 ttaatgtcgc cctgagaggc gacgaataga aaggagaagg gtgctccgta tttgctgaac
    15181 tgaatacggg ctacggatgg tgctaaaaag atagttattc ctagaactga cagttcttcg
    15241 tcataactcc tattttagct ttgtccgctt gacgccctta atatcttata tatgcctaaa
    15301 cgtactgata ttcaaaaaat tatggtgatt ggttctggtc cgattattat tggtcaggct
    15361 gctgagtttg actacgctgg gactcaggct tgcttgtcgt tgaaagagga aggttatgag
    15421 gttgttttgg ttaactcaaa tcctgcaacc atcatgacgg acaaggaaat tgctgacaag
    15481 gtttacattg aaccgattac acttgagttt gtgacacgta ttcttcgtaa ggaacgtcca
    15541 gatgccttgc taccaacact cggtggtcag acaggtctca acatggccat ggaattgtct
    15601 aaaaatggta tcttagatga gcttggcgtt gaacttctgg gtaccaaatt atctgccatt
    15661 gaccaagcgg aggaccgtga cctctttaaa caattgatgg aagagcttga acaacccatt
    15721 ccagaatctg aaattgttaa cacagtagaa gaagcagttg cctttgcagc gacaattggc
    15781 tacccagtca tcgttcgtcc agcctttacc ctcggtggta ctggtggtgg tatgtgtgcc
    15841 aatgagaaag aattgcgtga aatcactgaa aatggtttga aattgtcacc tgttacccaa
    15901 tgtttgattg agcgttcgat tgccggtttc aaggaaattg aatacgaagt gatgcgtgac
    15961 tcagctgaca atgccttggt tgtttgtaac atggaaaact ttgacccagt tgggattcac
    16021 acaggggatt ccattgtatt tgcccctgcg caaaccatgt cagactatga aaaccaaatg
    16081 cttcgtgatg cgagcttgag cattattcgt gccctcaaga ttgaaggtgg atgtaatgtt
    16141 cagctagctc ttgatcctaa cagcttcaag tattatgtta tcgaagtaaa ccctcgtgta
    16201 tcacgttcgt cagcccttgc ttctaaagcg actggatatc cgattgctaa gttggctgcc
    16261 aagattgcag taggtttgac cttggatgag gttataaacc cagttacagg ttcaacctat
    16321 gccatgtttg aaccagccct tgactacgta gttgctaaga ttccacgttt cccatttgac
    16381 aagtttgaaa agggtgaacg ccgtctcggt acacaaatga aggcgactgg agaagtcatg
    16441 gcaatcggtc gtaatatcga ggaatctctt ctcaaggcct gccgctccct tgaaattggg
    16501 gtgcaccaca atgaaatacc tgaacttgca gcagtttctg atgatgcctt gattgaaaag
    16561 gttgtcaaag cccaagatga ccgtctcttc tacgtatcag aagcaattcg tcgtggctac
    16621 acacctgaag aaattgcaga gcttacaaaa attgatatct tctatcttga taaactcttg
    16681 catatctttg aaattgagca agaattgggt gctcatccac aagatctaga agttttgaca
    16741 acagccaaac ttaatggttt ctcagaccgt aagattgctg aactctgggg aacgactgat
    16801 gataaagttc gccaacttcg cttggaaaac aagattgttc cagtttataa gatggtagac
    16861 acttgtgcgg cagagttcga ctccgaaacg ccatatttct attcaaccta tggttgggaa
    16921 aatgaatcta tcagatctga taaagaatct gtacttgtcc taggttcagg tccaatccgt
    16981 atcggtcaag gggttgagtt tgactacgca accgttcact ctgttaaggc tatccaggca
    17041 gctggttatg aagctatcat catgaactca aacccagaga ccgtttcaac agacttctct
    17101 gtatcagata agctttactt tgagccattg acattcgaag atgttatgaa cgtcattgac
    17161 ttggaacaac caaagggagt tatcgttcag ttcggtggtc aaacagccat caaccttgcg
    17221 gagccattgg caaaagcagg tgtgaccatc cttggtacac aggttgctga cctagaccgt
    17281 gccgaagacc gtgacctctt cgagcaagct ctcaaagaat tggatattcc acagccacca
    17341 ggacaaacgg ctaccaatga agaagaagca gctcttgcag ctcgtaagat tggcttccca
    17401 gttcttgtcc gcccatctta tgtacttggt ggacgtgcca tggaaatcgt tgaaaacgaa
    17461 gaagaccttc gttcttacat gcgaactgct gttaaggcta gtccagacca tccagttctt
    17521 gtcgactctt atatcgttgg gcaagagtgc gaagttgatg ccatttcaga tggtaaaaat
    17581 gtactcattc ctggtatcat ggagcatatc gaacgtgctg gtgtccactc aggtgactca
    17641 atggctgttt acccaccaca aaccttgtcg caaaaggttc aggagaccat cgcagactac
    17701 accaaacgct tagcaatcgg tcttcactgc cttggaatga tgaacatcca gtttgttatc
    17761 aaggatgaaa aagtctacgt tattgaggtc aatccacgtg ccagccgtac cgttccattc
    17821 ctatctaagg taaccaatat tcctatggct caagtagcga ccaagctcat tcttggtcaa
    17881 agtttatcag aacttggcta ccaaaatgga ctttaccctg aaagcacccg tgttcatatc
    17941 aaggcacctg tcttctcctt taccaaacta gctaaggtag acagcttgct cggtcctgaa
    18001 atgaagtcaa caggtgaagt tatgggttct gatgctactt tggaaaaagc tctctataaa
    18061 gcctttgaag cttcttatct acacttgcca acttttggta atgtcgtctt taccatcgct
    18121 gatgatgcca aagaagaagc cttgaacttg gctcgtcgtt tccaaaacat tggttatgga
    18181 atccttgcga cagaagggac agcagccttc tttgctagtc atggcttgca agcccaacct
    18241 gttggtaaga ttggtgatga cgataaggat atcccaagtt ttgtacgcaa aggaagaatt
    18301 caagctatca ttaacacagt aggaacaaaa cgaactgctg acgaagatgg tgagcaaatt
    18361 cgtcgttcag ccattgaaca cggggtgccc ctcttcacag cgctagatac agctaatgcc
    18421 atgcttaagg tacttgaaag tcgtagcttt gttacagaag cgatttaaat agaaaaataa
    18481 ggtatagcag actgaattta gaacagtaca cagggatact aaaatatttc tagaaattaa
    18541 tttgaatttt ctaattgatt tgttcgcatc ttatttcaat ctactatatc atcttcatcc
    18601 agtttcgtaa aagaaaaaac tctaattaca gatacgaatt agagttcagc ttacaagatt
    18661 agacagttct tttcgacata cgaaaaaaac atttcacatt tcccttcgcc agtcttaact
    18721 gtatcaggtt caatgggtat catctcagcc taaagcaccc caaatgtctt tattatttaa
    18781 ttatgtgatt attataacac acattttata ctagttcaag aaattgaact ggaaatacag
    18841 ccttgcactc acaaagacag cagatctttc ttttgcaaaa aacaaatgac ccgtttgatg
    18901 aattagccat tcaagctgaa tctggacata gctttttaaa aaaggaaaat cctacttact
    18961 tagaatccaa ggatagatat ctattgttca ctcatttccc gaacagtttt ttctatattt
    19021 tttgcatacg atattgccga aatgattgaa acgccatcca tattggtctt tataatgtct
    19081 ttaatatgtt tcgtctgtat cccaccaatt gcaactaaag gcatttgtgg caatagtttt
    19141 ctcatcaatt caagaccttc ataacctata gtaccaccag catcatcctt tgactgggta
    19201 ccaaatacag gcccaacacc tacataatct acatattcaa cttttgattg ttgaaattct
    19261 tcttcgtttc ttatagaaag accaattatt ttatctggca tcaattttct aatttcatca
    19321 acaccaatat catcttgacc tacatgtacg ccatcggcgt caatttccat tgctaaatct
    19381 atatcgtcat taacgataaa tggaacattg tattttttac aaagttcttt aatttggata
    19441 gctagctcaa gtttttctaa gccttctaaa gcaccctcac ctttttctcg aaattgaaat
    19501 aaggttatac caccttttaa ggcttcctca acgactgtat atagattttt tccttggcaa
    19561 gtagtcgttc cacaaataaa atatagtttt agtaattctt tatgaaacat cttacttcac
    19621 tcttttgaat tcctttacat cttcatctgt aatctcgtat aaggcattta taaattcaac
    19681 tttaaatgtc ccaggaagat gtccatttgg acgtttttct gccatttctc cagcgatatt
    19741 gtaaaccaac attgctgttt ctaatgattt caattcttga cctttttcta gtccgataaa
    19801 gcttgctatc actgccccta ataagcatcc tgtcccaatg actttcggca tcatagcact
    19861 accattatga atcgttacca cttctccatt aacagcaatg gcatccactt cacctgttac
    19921 tactattgga atattgaact tctcatttgc tgctagagca atttcgtcaa tattatctac
    19981 gcccgcacta tctactcctt tagatgccac atctattcct actaaagagg caatctcgcc
    20041 agcatttcct ctaatcgctg ctagtttata attgttgatt agatcatctg ctactttttt
    20101 tctatattct cctgctccac aggctacagg atctaaaact gctgggacat tatatttctc
    20161 tgcaattttc agagcagctt ggtataattt ccaattttca tctgtcaatg ttcctatgtt
    20221 tattaataaa ccaccagcat actttaacaa atcctctaaa tctgctggaa actcactcat
    20281 ggctggtgag gcgcccagtg ctactaatcc atttgctgtg aaatttttta ctacatcatt
    20341 ggttatacaa atgaccaatg gtgctttttc ttttaataat tttaaacttg tcatattgaa
    20401 atccttcctt ttcactttat acgatctact gatttcgatt tatttctatc tttcgttaag
    20461 aatttttttc atttacattg aatgatttat actcaatgaa aatcaaagag caaactagga
    20521 ggctaaccgc aggttgctca aaacactgtt ttgaggttgt ggatagaact gacgtggttt
    20581 gaagagattt tcgaagagtc ttacctcatc aaatttgtaa atatcatgag ccttctctag
    20641 acatcgtaac caatatcaaa aaaagctaat tctaaagcga ctgcttgatt ccagcgttgc
    20701 tgaagttctg tcaaatcttc tcgattttta ccgacacgat tgagttcgtc aaccagaaat
    20761 tgaacccact ctgcaaagaa aggacctctg tggagattga tccattccga atgaatatag
    20821 acttcaggta aagccaaatc tttagaaccc cagtctaaat agagaccttc tgcaatgacc
    20881 agcatgacca aaagatgggc atagtctgat gaagccaccg ccgaatacat tagatcctga
    20941 aaggcttttg ttacagggtg caaagtcact tctagatagt cattctctgc tacttttaac
    21001 tctttaaaag ccttttggaa ataaccatct tcatctgctt caagaaagcc tagttgcttg
    21061 gcaaaacgaa gcttggattc aagtttatct gcgtgagcta cgcaggcacc cagcatggat
    21121 aagaaggcat caaagaagtg ataatcttga atcagatagt cctttaagac cttattctca
    21181 attgtccccg caaaaagttc cttaacaaaa cgatgattga ttgcagcctg ccaatccttc
    21241 tgactgcttt ttaataattc tccaacagtc aaacctggct gaaatgcata gtcttgtgtt
    21301 tccatattta cttctcctct ctttacttgt tagtaattaa taaaacacca agaaatatca
    21361 agcaaaatcg taattccact tgatcctttt aaagcacatc gagagcattt gcagagagct
    21421 aactaaacaa gcctatccag tttatataaa caaaaaactc caattacaat caagaattag
    21481 agttgactta caagattaga tcgttcattt cgccatacga aaaaacgttt cacatttccc
    21541 ttcgccagtc ttaactgtat caggttcaat gggtatcatc tcagcttaaa gcaccccaaa
    21601 tgtctctatt atttaattac tgaaccagta tagcaaaaaa tgaaagccct agcaagatat
    21661 ttgaccgaaa aatatcttta tatagta
//
LOCUS       DAVKJD010000028        21157 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKJD010000028 DAVKJD010000000
VERSION     DAVKJD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.028"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            86..2308
                     /gene="recJ"
                     /locus_tag="VW157_001072"
     CDS             86..2308
                     /gene="recJ"
                     /locus_tag="VW157_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9552387.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSIPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2483..4144
                     /locus_tag="VW157_001073"
     CDS             2483..4144
                     /locus_tag="VW157_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9552388.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAICLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4213..4992
                     /locus_tag="VW157_001074"
     CDS             4213..4992
                     /locus_tag="VW157_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9552389.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5104..6324
                     /gene="murM"
                     /locus_tag="VW157_001075"
     CDS             5104..6324
                     /gene="murM"
                     /locus_tag="VW157_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9552390.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6329..7561
                     /gene="murN"
                     /locus_tag="VW157_001076"
     CDS             6329..7561
                     /gene="murN"
                     /locus_tag="VW157_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9552391.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIYAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7640..8341
                     /locus_tag="VW157_001077"
     CDS             7640..8341
                     /locus_tag="VW157_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9552392.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGFLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8381..10225
                     /gene="uvrC"
                     /locus_tag="VW157_001078"
     CDS             8381..10225
                     /gene="uvrC"
                     /locus_tag="VW157_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9552393.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKIFKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNKP"
     gene            10215..11048
                     /locus_tag="VW157_001079"
     CDS             10215..11048
                     /locus_tag="VW157_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9552394.1"
                     /translation="MNHKIAILSDIHGNVTALEAVIADAKNQGVSEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQYLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNILFRKV
                     AYDYEAELEFAKSKGLPFIEMYEELRREDDYQRHNLELLASLIEKHRYVEDVKNFFDF
                     L"
     gene            complement(11230..12030)
                     /locus_tag="VW157_001080"
     CDS             complement(11230..12030)
                     /locus_tag="VW157_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9552395.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12201..12806
                     /locus_tag="VW157_001081"
     CDS             12201..12806
                     /locus_tag="VW157_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9552396.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            12819..14219
                     /gene="pepV"
                     /locus_tag="VW157_001082"
     CDS             12819..14219
                     /gene="pepV"
                     /locus_tag="VW157_001082"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9552397.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDRKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAEDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14273..14851
                     /locus_tag="VW157_001083"
     CDS             14273..14851
                     /locus_tag="VW157_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9552398.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15011..15163
                     /locus_tag="VW157_001084"
     CDS             15011..15163
                     /locus_tag="VW157_001084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9552399.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            15206..15298
                     /locus_tag="VW157_001085"
     CDS             15206..15298
                     /locus_tag="VW157_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9552400.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15469..16794
                     /gene="brnQ"
                     /locus_tag="VW157_001086"
     CDS             15469..16794
                     /gene="brnQ"
                     /locus_tag="VW157_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9552401.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVIGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16892..17929
                     /locus_tag="VW157_001087"
     CDS             16892..17929
                     /locus_tag="VW157_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9552402.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDGQQTDEYTVSICVKDASGPYHYDFRQHLVTLA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYIKSALVD"
     gene            17931..18392
                     /locus_tag="VW157_001088"
     CDS             17931..18392
                     /locus_tag="VW157_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9552403.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMKGHGLNGRGPVWWVPFEEMTAETCQVKPDEIERLVKRLLIEVDKLLK"
     gene            18403..19119
                     /locus_tag="VW157_001089"
     CDS             18403..19119
                     /locus_tag="VW157_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9552404.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGEAKTEQTAKADGTVG
                     SKSQGTAQKRAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AESGLSLEEYYGFEGGDYVD"
     gene            19242..19667
                     /gene="rplK"
                     /locus_tag="VW157_001090"
     CDS             19242..19667
                     /gene="rplK"
                     /locus_tag="VW157_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9552405.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            19875..20564
                     /gene="rplA"
                     /locus_tag="VW157_001091"
     CDS             19875..20564
                     /gene="rplA"
                     /locus_tag="VW157_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9552406.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            20942..21100
                     /locus_tag="VW157_001092"
                     /pseudo
     CDS             20942..21100
                     /locus_tag="VW157_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         6341 a         3901 c         4922 g         5993 t
ORIGIN      
        1 ttataaaaaa gcaatctgga tagagaaaac gtctaaatca tgttataatg aagcaataga
       61 attcttagaa agagtggatg tctttttgat aacacctact tatgaatggc agtttgccct
      121 gcaggtagaa gatgcggatt ttacaaagat agccaagaaa gctggactgg gtcctgaggt
      181 agctcggtta ttgtttgaga gagggattca ggaccaagaa agtctgaaga agtttttaga
      241 accttccttg gaggacttac atgatgctta tctgctccat gatatggaca aggcagtgga
      301 gcggattcgt caggctattg aagaagggga aaatattctc gtttatggag actacgatgc
      361 ggatggcatg acttcggctt ctattgtgaa ggaaagtttg gaacaacttg gtgctgagtg
      421 ccgagtttac ctgccaaatc gttttaccga tggctatggc cctaatgcta gtgtttataa
      481 atactttatc gagcaagaag ggatttcctt gattgtgacg gtggacaatg gggttgctgg
      541 tcatgaggct attgcattgg ctcagtctat gggagtagat gtcattgtga cagaccatca
      601 ttccatacct gaaaccctgc cagatgctta tgctattgtc catcctgaac atccagatgc
      661 ggattatcct tttaaatatt tggctggttg tggagttgct ttcaagttgg cttgtgccct
      721 gttagaagaa gtgcaagtgg aattgcttga tttggtcgct attggaacta ttgcagatat
      781 ggtgagtctg acggatgaaa atcgtatctt agttcaatat ggtctggaaa tgttgggtca
      841 tacccagcgc attggtctgc aagaaatgct ggacatggct gggattgctg ccaacgaagt
      901 aacagaagaa acggttggtt tccagattgc tcctcgtttg aatgccttgg gccgcttgga
      961 tgatcccaat cctgccattg atttgttgac tggatttgat gatgaggaag cgcatgagat
     1021 tgcccttatg attcaccaga aaaacgaaga gcgcaaggaa atcgttcagt ctatctatga
     1081 agaagccaag accatggtgg atcctgagaa gaaggttcag gtcttggcca aggaaggctg
     1141 gaatcctggg gttctaggaa tcgtggctgg tcgtttattg gaagaattgg gacagacagt
     1201 cattgttctt aatatagaag acggtcgtgc caagggcagt gctcgtagtg tggaagcggt
     1261 cgatattttt gaagctctgg atccccatcg agacctcttc atcgcctttg gaggtcatgc
     1321 aggtgcagcg ggtatgacgc tggaagttga gcaactctca gatttatctc aggttttgga
     1381 agattatgtt cgtgaaaaag gtgcagatgc tggtggcaag aataagttaa acctagatga
     1441 agagttggat ttggaggcac ttagcttgga aacggtcaaa agttttgaac gtttagctcc
     1501 ttttggaatg gataatcaga aacctatttt ttatatcaag aattttcagg tcgaaagtgc
     1561 tcgtactatg ggggcaggta atgcccatct aaagctgaaa atttccaagg gtgaggcgag
     1621 ttttgaagtg gtagcctttg gtcaaggcag atgggcgaca gagttttctc aaaccaagaa
     1681 tctagagtta gcggttaaat tgtctgtcaa ccaatggaat ggccaaactg ccctccagtt
     1741 gatgatggtg gatgcgcgag tggaaggtgt tcaacttttt aacattcgtg gaaaaaatgc
     1801 agtcttgcca gaaggtgttc cagtcttgga ttttcctgga gaactgccaa atcttgcggc
     1861 tagtgaagct gttgtcgtaa aaaacattcc agaggatatt actcagctga agaccatttt
     1921 tcaggaacag catttctctg ctgtctattt caaaaatgat attgacaagg cttattatct
     1981 gacaggttat gggactagag atcagtttgc caaattgtac aagactattt accagttccc
     2041 agagtttgat attcgctaca agctgaaaga tttggctgca tatcttaata ttcaacaaat
     2101 cttgctggtc aagatgattc aagtatttga agaactaggc tttgtgacga taaaagatgg
     2161 tgtgatgaca gtcaataaag aggcgccaaa gcgggagata ggagaaagtc aaatttacca
     2221 aaatctcaaa caaaccgtta aagaccaaga aatgatggcg ctgggtacgg tgcaagaaat
     2281 ttatgatttt ttgatggaaa aagagtagaa gttaggaaag agttgggaaa tcaactcttt
     2341 tttgaaaaca gaccttcatt ttgaaaatca tcaaaaaaat ggtataatgg taggaaaaga
     2401 ttcggctgaa agtatcagaa cttttagaat aagagggtag aattgcccta taatcaagat
     2461 aaactaagat tttggaggaa aaatgagtaa tatcagttta acaacacttg gtggtgtgcg
     2521 tgagaatgga aaaaatatgt acattgctga aattggagag tccatttttg ttttgaatgt
     2581 agggttaaaa tatcctgaaa atgaacaatt aggggtcgat gtggtgattc caaacatgga
     2641 ttaccttttt gaaaatagcg accgtattgc tggggttttc ttgacccacg ggcatgcgga
     2701 tgcgattggt gctctacctt atctcttggc agaggctaaa gttcctgtat ttgggtctga
     2761 gttgaccatt gagttggcaa agctctttgt caaaggaaat gatgccgtta agaaatttaa
     2821 tgatttccat gtcattgatg agaatacgga gattgatttt ggtgggacag tagtttcctt
     2881 cttccctacg acttactccg ttccagagag tctgggaatt gtcttgaaga catcggaagg
     2941 aagcatcgtt tatacaggtg acttcaaatt tgaccaaacg gctagtgaat cttatgcaac
     3001 tgattttgct cgtttggcag agattggtcg tgacggcgtc ctggctctcc tcagtgattc
     3061 ggccaatgca gacagcaata ttcaggtggc tagtgaaagt gaagttaggg atgaaattac
     3121 ccaaactatt gctgactggg aaggtcgtat catcgttgca gctgtttcca gtaatctttc
     3181 tcgtattcag cagatttttg acgctgcgga taaaacaggt cgacgtatcg tcttgacagg
     3241 atttgatatt gaaaatatcg tccgcacagc gatttgtctt aagaagttgt ctttagccaa
     3301 cgaaattctc ttgattaagc ctaaagatat gtctcgcttt gaagaccatg agttgattat
     3361 tcttgagaca ggtcgtatgg gtgaacctat caatggactt cgtaagatgt cgattggtcg
     3421 ccatcgttat gtagaaatca aggatgggga cctggtctat attgctacgg ctccgtctat
     3481 tgctaaagaa gcctttgttg cgcgtgtaga aaatatgatt tatcaggcag gtggggttgt
     3541 caaattgatt acccaaagtt tacatgtatc agggcacgga aatgtgcgtg atttgcagct
     3601 gatgatcaat cttttgcaac ctaagtacct cttccctgtc caaggggagt atcgtgagtt
     3661 ggatgctcac gctaaggctg ccatggcagt tgggatgttg ccagaacgca tcttcattcc
     3721 taaaaagggg acgaccatgg cttacgagaa tggagacttt gttccagctg gatcggtttc
     3781 agcaggagat atcttgattg atgggaatgc cattggtgat gttggaaatg ttgttcttcg
     3841 tgaccgtaag gtcttgtcag aggatggaat tttcatcgtg gctattacag tcaaccgtcg
     3901 tgagaagaaa attgtggcta gggctcgtgt tcatacgcgc ggatttgttt atctcaagaa
     3961 gagtcgcgat attctccgtg aaagttcaga attgattaac caaacggtag aagattatct
     4021 tcaaggagat gactttgact gggcagatct taaagggaag gttcgagata atttgaccaa
     4081 gtatctcttt gaccaaacca agcgtcgtcc agctatttta ccagtagtca tggaagcaaa
     4141 ataatcgttg aaataaacag agagaaagtc gagtttcggc tttttcttat agaaaaatag
     4201 aaggagaaaa tcatggcagt aatgaaaatc gagtattact cacaagtatt ggatatggag
     4261 tggggggtga atgtcctcta ccctgatgcc aatcgagtgg aagaaccaga gtgtgaagat
     4321 attcccgtct tgtacctttt gcacgggatg tctggaaatc ataatagttg gcttaagcgg
     4381 accaatgtag aacgcttgct tcgaggaact aatctcatcg ttgttatgcc caataccagc
     4441 aatggttggt acaccgatac ccagtatggt tttgactact acacggctct agcagaggaa
     4501 ttgccacagg ttctgaaacg cttcttccct aatatgacta gcaagcgtga aaagaccttt
     4561 atcgctggtc tttctatggg aggctacggc tgcttcaaac tggctcttac gacaaatcgt
     4621 ttttctcatg cagctagttt ttcaggtgcc ctcagctttc aaaacttttc tcctgaaagt
     4681 caaaatctgg gaagtccagc ctactggaga ggtgtttttg gagagattag agactggaca
     4741 actagtccct attctcttga aagtctggct aaaaaatcgg ataaaaagac caaactttgg
     4801 gcgtggtgtg gcgaacagga tttcttgtac gaagccaata atctcgcagt gaaaaatctc
     4861 aaaaaactag gttttgatgt gacctatagc catagtgctg gaactcacga gtggtactac
     4921 tgggaaaaac aattggaagt ttttttaaca accctaccaa ttgatttcaa attagaagag
     4981 agactgactt agtttgaact tcagcatagg gggagtagaa ctaaaataaa atatgttttc
     5041 actagacttt tcaaacgaaa gtagtagaat agtaataaaa tactggagga aagagagtag
     5101 gaaatgtacc gttatcaaat tggcattccc acattagaat atgatcagtt tgtcaaagaa
     5161 catgaattag ccaatgtatt acaaagtagt gcttgggagg aagttaagtc taattggcaa
     5221 catgagaagt ttggtgttta cagggaagaa aaattactgg cgacagctag tattttgatt
     5281 agaactcttc cgctaggcta taaaatgttt tacatcccaa gaggacctat attggattat
     5341 ggggataaag aactcttgaa ttttgccatt cagtctatta agtcctatgc tcgcagtaag
     5401 agagcggttt ttgtgacttt tgacccaagt atttgcctat ctcaaagttt aatcaatcag
     5461 gaaaagacag aatttcctga aaatctggct attattgata gtttgcaaca aatgggagta
     5521 aggtggtcag gaaaaacgga ggaaatggga gacaccattc aacctcgtat tcaggcgaaa
     5581 atatacaagg aaaattttga agaagataaa ctttccaagt caacaaaaca ggctattcga
     5641 acagcacgaa acaaagggct tgagattcaa tatggtggac tggaactatt agattcattt
     5701 tcggagttga tgaaaaaaac tgagaagcga aaagagattc atttgaggaa tgaagcctat
     5761 tataaaaaat tgttagataa ttttaaggac aaggcctata tcaccttggc caccttggat
     5821 gtttctaaac gttcgcaaga attagaagaa cagttagcga aaaatagagc cttggaagag
     5881 acctttactg agtcgactcg aacttcaaaa gtagaagcgc agaagaagga aaaagaacgt
     5941 ttgttagagg aattgacctt cttgcaggaa tatatagatg taggtcaagc gagagttcct
     6001 ttagcggcta ctttgagttt ggaatttggt actacctctg tcaatatata tgctggtatg
     6061 gatgatgatt ttaaacgtta caatgcacca attttaacat ggtatgaaac ggctcgctat
     6121 gcctttgagc gaggtatggt ctggcaaaat ttaggtggtg ttgaaaactc tctcaatggt
     6181 ggactttatc attttaagga aaaatttaat ccaacgattg aagaatactt gggtgaattt
     6241 acaatgccca ctcatcctct ctatcctctg ttaagacttg ctcttgattt ccgtaaaaca
     6301 ttaagaaaaa aacatagaaa gtaagtatat ggcactaaca acactcacga aagaagagtt
     6361 tcagacttat tctgatcagg tttcttctcg ttcctttatg caatctgtcc agatggggga
     6421 tttgctagaa aaaagagggg ctcgaattgt ttatcttgct ttgaaacaag aaggagaaat
     6481 tcaagttgca gctctggttt atagcttgcc catgctgggt ggtctgcata tggaactcaa
     6541 ttcggggccg atttataccc aacaagatgc tcttccagtt ttttatgcag agttaaaaga
     6601 atatgccaag caaaatggtg tattagagtt gcttgtaaaa ccttatgaaa cttatcaaac
     6661 ttttgatagc caaggtaacc caatatatgc tgagaaaaaa agtattattc aaggtttgac
     6721 tgatttaggt tatcaatttg atggcttaac aacaggttac ccaggtggag aaccagattg
     6781 gttatactat aaagatttaa ctgaattaac tgaaaagagt ttgcttaaaa gttttagcaa
     6841 aaagggtaaa cccttggtga aaaaggctga aacctttggc attcggttga aaaagttaaa
     6901 acgtgaagaa ctatcgattt ttaagaatat aacaaaagaa acctctgaac gtagagaata
     6961 tagtgataaa agtttagaat attatgagca tttttatgat acttttggag aacaagcgga
     7021 gtttctcata gcaagcttga atttttcgga gtatatgagc aaattgcaag gtgaacaaag
     7081 taaactagaa gaaaacttgg acaagttgcg acttgatttg agtaaaaatc ctcattctga
     7141 gaaaaaacaa aatcaactga gagaatattc tagtcaattt gaaacgtttg aagttcgaaa
     7201 agcagaagcg cgagacttga ttgaaaaata tggagaagaa gatattgttt tagctgggag
     7261 tttatttgtt tatatgcctc aggaaacgac ttatctcttt agtggttcct acactgagtt
     7321 taataagttc tatgcccctg cacttcttca aaaatatgtt atgttggaaa gcataaaacg
     7381 tggaatacct aaatacaact tcctaggcat tcaagggatt tttgatggaa gtgatggtgt
     7441 tttgcgtttt aaacagaatt ttaatggcta tattgtacgc aaagcaggta ctttccgtta
     7501 ccatccatcg cctttaaaat acaaagctat ccagttactc aaaaaaatag taggacgtta
     7561 agatgaaaaa gtcagtattt agatttcttt tagcttcttt tagtaaaata atttttattt
     7621 gctagaaagg tggagagaca tgcgctggct ttttcgtttg ataggggctt tcttttcttt
     7681 tgtgtggcgt ttgttttggc gtctggtttg gatagttgtg ctcttatgtg tgcttgcttt
     7741 cggatttctc tggtatctga acggggattt tcaaggagcg ctaaagcaag cagaacggtc
     7801 agtaaaaatt ggtcaacaaa gtattgacca atgggagaaa acagggcaac tgcctaagtt
     7861 aagccagaca gatagtcacc agcattctga aggaaggtgg gcacaggcct ctgctcgtat
     7921 ttacctggat ccgcagatgg attcacgctt tcaagaggct tatttagaag caatccagaa
     7981 ctggaatcaa actggtgctt ttaactttga actcgtgact gagtctagta aggcggatat
     8041 tacggctacg gagatgaacg acggaggcac tcctgtggca ggagaggcgg aaagtcaaac
     8101 taatctctta acagggcaat tcttgtccgt aacggtgcgg ttgaatcatt attatttgtc
     8161 caatccatac tatggctact cctatgaacg ccttgtccat acggcagaac atgagttagg
     8221 tcatgcgatt ggcttggacc atacagatga gaagtctgtc atgcaaccag caggttcctt
     8281 ttatggtatc caggaagagg atgttgcaaa cctccgaaaa atatatgaga ctagtgagta
     8341 gggtactatc tttccctact ttttttgcta taatggaact atgaacaact tgattaaatc
     8401 aaaactagag ctcttgccga ccagccctgg ttgctacatt cataaggata aaaatggcac
     8461 cattatctat gtaggaaagg ctaaaaatct gcgtaatcga gtacggtcct attttcgtgg
     8521 aagtcatgat accaagacag aggctctggt gtctgaaatt gtggattttg aatttattgt
     8581 tacggagtct aatattgagg cacttctcct agaaatcaac ctgatcaagg aaaacaagcc
     8641 caagtacaat atcatgctca aggatgacaa gtcctatcct ttcatcaaaa tcaccaatga
     8701 gcgctatcca cgcttgatta tcactcgtca ggtcaaaaag gacggaggtc tttattttgg
     8761 accctatccc gatgtggggg cagccaatga aatcaagcgg ttgctggatc ggatattccc
     8821 ttttcgtaag tgtaccaacc cgccctctaa ggtctgtttt tattaccata tcggtcaatg
     8881 catggcccac accatctgta agaaggatga ggcctatttc aagtctatgg cccaggaggt
     8941 ttctgatttt ctgaaagggc aggataacaa aatcattgat gagctaaagg gcaaaatggc
     9001 agcggcagcc cagactatgg agtttgaacg tgcggcggaa taccgtgacc tgattcaggc
     9061 tattggaacg cttcgaacca agcaacgggt catggctaag gatctccaaa atcgcgatgt
     9121 ctttggctac tatgtggata agggctggat gtgtgtgcag gttttctttg tccgtcaggg
     9181 taagctcatc gagcgcgatg tcaatctctt cccctacttc aatgatccag atgaggattt
     9241 tttgacctat gtaggacaat tctatcaaga aaaatctcat ctagttccca atgaggtact
     9301 gattccgcag gatattgacg aagaagctgt caaggccttg gtggatacca agattttcaa
     9361 accccaacgt ggagagaaaa aacagctggt caatttagcc ataaaaaatg cacgtgttag
     9421 tctggagcag aaatttaacc tgctagaaaa atcagtcgaa aagacccaag gagctattga
     9481 aaatctagga cgcttgcttc aaatcccaac cccagttcgc atcgagtcct tcgataactc
     9541 caacatcatg ggaactagtc ctgtttcggc tatggtggtc tttgtcaacg gtaaaccgag
     9601 taagaaggat taccgtaagt acaagataaa aacggttgtt ggaccagacg actatgccag
     9661 catgagagag gtcattcgca gacgctatgg tcgagtacag cgtgaggctt tgactcctcc
     9721 agatttgatt gtgattgatg gggggcaagg tcaagtcaat atcgctaagc aggttatcca
     9781 agaggaactg ggcttggata ttccaattgc aggtctgcaa aagaatgata agcaccaaac
     9841 ccatgaattg ctctttggag atccgctgga agtggtggac ttgtctcgca attctcagga
     9901 atttttcctc ctccaacgca tccaagatga ggtgcaccgc tttgccatca ccttccaccg
     9961 ccaactgcgc tccaaaaatt ctttctcatc tcaattggat gggattgatg gtctgggacc
    10021 taaacgcaag caaaatctta tgaagcattt caagtctttg accaaaatca aggaagccag
    10081 tgtggatgag attgtcgaag ttggggtacc tagagtcgtt gcagaggctg tgcaaagaaa
    10141 gttgaacccg cagggagaag ccttgcctca agtagcagaa gaaagagtag attaccaaac
    10201 ggaaggaaac cacaataaac cataaaatcg caattttatc agatattcat ggcaatgtga
    10261 cggcgctaga agcagtgatt gcagatgcta aaaatcaagg agtcagtgaa tactggcttc
    10321 tgggagatat ttttcttcct ggtccaggcg caaatgactt agtcgccctg ctaaaggacc
    10381 ttcctatcac agcaagtgtt cgaggcaatt gggatgatcg tgtccttgag gctttagatg
    10441 ggcaatatgg cttagaagac ccacaggaag ttcagctctt gcgtatgaca cagtatttga
    10501 tggagcgaat ggatcctgca acgattgtct ggctacgaag cttgcctttg ctggaaaaga
    10561 aagaaattga cggattgcgc ttttctatct ctcataattt acctgacaaa aactatggtg
    10621 gtgacttgct agttgagaat gatacagaga aatttgacca actgctagat gcggaaacgg
    10681 acgtggcagt ttatggtcat gttcacaagc agttgcttcg ttatggtagt caagggcaac
    10741 aaatcatcaa tccagggtcg attggcatgc cctattttaa ttgggaggcg ttaaaaaatc
    10801 accgtgccca gtatgccgtg atagaggttg aagatgggga attggtaaat atcctatttc
    10861 gtaaagtcgc ttatgattac gaggctgagt tagaatttgc caaatccaag gggcttccct
    10921 ttatcgaaat gtatgaagaa ctacgtcgtg aagatgacta tcagaggcac aatctggaac
    10981 tattagcaag cttaatagaa aagcataggt atgtagagga tgtgaagaat ttttttgatt
    11041 ttttgtaaga gtttcctaaa ataaccaatg caaactaaaa aagcgattgg ctgatcccat
    11101 cgcttttagt atatcttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgta
    11161 ggttgctcaa agcacagctt tgaggttgca gataaagctg acgtggtttg aagagatttt
    11221 cgaagagtgt tattgtaact gagattgatc tgggaggtaa gaaccaccta gataggtatt
    11281 gctgagtttt tcaagggttc cgtcttgata gagttctttg agcgctttat caaattgctc
    11341 tttaaactct ttttggtcgc ttgagaaaat gatataattg ctggggctat ctgcagaagg
    11401 taaatcaacg actgagaggt ctaaaccacg gtccttgata atcttttgaa cggatacctt
    11461 gtcaaaaact aggaaatcaa actctccgtt agcaaggtct aggattcgtt taccaatatc
    11521 ctcaccagaa aaattaattg tagcgggatt atcagtgtgt ttctgattcc agttattgat
    11581 gaattgagcg ttagaagttc cggtatcctc ttgtgttgtt ttaccagcga tctggtcaag
    11641 agaagtcaaa ggatttttct tgttgctgac aaggacgagg ggattgttgg aaattggaag
    11701 cgagtaaagg tatttttcag cacgctcttt tgtgtaactc aagttattgg ccgcagcctg
    11761 atagtgacca gaatcaagtc ctgggaagat gctctcccag gcggttcttt ggaattgaat
    11821 ctcgtagtcg ctgagttttt catctactgc ctttaaaact tcgatatcga agcctgtcag
    11881 attgcccttg tcttcgtagt caaatggtgg cacgtcgcca gctgtagcaa ggacgattgt
    11941 cttttgagcg ctagtctctt tgggtgtagc ttgattccca caggcaacca aaaatggtag
    12001 gatagctagt aataggctaa attttttcat actgtctcca ttcaaatgta aagtacttgc
    12061 tagtttatca gaaactttcc tgataaacca atatatatct tttatgtctg tgatagaaaa
    12121 acttaatcat gaaaaatccc tgcaagctct ttcaaattat ggtagaatgg aagcagtaga
    12181 attagaaaag gaaatcgatt atgaaatttc ttgaattaaa taaaaaacgt catgcgacta
    12241 agcattttac tgataagcct gttgatccca aagatgtgcg tacggctatc gaaattgcaa
    12301 ccttggcgcc aagcgcccac aacagccagc cttggaaatt tgtggtggta cgtgagaaaa
    12361 atgctgaact ggcaaagtta gcttatggtt ccaattttga acaggtatca tcagcgcctg
    12421 taaccattgc cttgtttaca gatacggact tagccaaacg tgctcgtaag attgcccgtg
    12481 ttggtggtgc taataacttt tctgaagagc aacttcaata ttttatgaaa aatctgccag
    12541 ctgagtttgc ccgttacagt gagcaacaag tcagcgacta cctagctctc aatgcaggtt
    12601 tggttgccat gaacttggtt cttgcattga cagaccaagg aattggttct aacattattc
    12661 ttggttttga caaatcaaaa gttaatgaag ttttggaaat cgaagaccgt ttccgcccag
    12721 aactcttgat cacagtgggt tacacagacg acaaattgga accaagctac cgcttgccag
    12781 tagatgaaat catcgaaaaa agataggaag aagaaaaaat gatagcaatt gattttacag
    12841 cagaagtaga aaaacgcaaa gaagacctct tggctgactt gtttagcctt ttggaaatca
    12901 actcagaacg tgatgataga aaggctgatg cccaacatcc atttggacct ggtccagtaa
    12961 aagccttgga aaaattcctt gaaatcgcag accgtgatgg ttatccaact aaaaatgttg
    13021 ataactatgc aggacatttt gagtttggtg atggagaaga agttctcgga atctttgccc
    13081 atatggacgt ggttccagct ggtagcggtt gggatacaga tccttacaca ccaactatca
    13141 aagatggtcg cctttatgcg cgcggggctt cggacgataa gggtcctaca acagcttgtt
    13201 actatggttt gaaaatcatc aaagaattgg gtcttccaac ttctaagaaa gttcgcttca
    13261 tcgttggaac agacgaagaa tcaggctggg cagacatgga ctactacttt gagcacgtag
    13321 gacttgccaa accagatttc ggtttctcac cagatgctga atttccaatc atcaatggtg
    13381 aaaaaggaaa tatcacggaa tacctccact ttgcaggaga aaatacaggt gctgcccgtc
    13441 ttcacagctt tacaggtggt ttacgtgaaa atatggtacc agaatcagca acagcagtcg
    13501 tttcaggtga tttggctgac ttgcaagcta aactagatgc ctttgttgca gaacacaaac
    13561 ttagaggaga actccaagaa gaagctggca aatacaaggt gacgatcatt ggtaaatcag
    13621 cccacggtgc tatgcctgct tcaggtgtca atggcgcaac ttaccttgcc ctcttcctca
    13681 gccagtttgg ctttgctggt ccagccgaag actaccttga catcgcaggt aaaattctct
    13741 tgaacgatca tgagggtgaa aatctcaaga ttgctcatgt ggatgaaaag atgggtgctc
    13801 tttctatgaa tgccggagtc ttccgcttcg atgaaacaag tgctgataat accattgccc
    13861 ttaacatccg ctatccaaaa ggaacaagtc cagaacaaat caaatcaatc ctagaaaact
    13921 tgccagttgc ttctgtcagt ctatcagaac atggtcatac ccctcactat gtgccaatgg
    13981 aagatccact tgtgcaaacc ttgttgaatg tctatgaaaa acaaacaggc ctacaaggtc
    14041 atgaacaagt catcggtggt ggaacctttg gtcgcttgtt agagcgcgga gttgcctatg
    14101 gtgctatgtt cccagactca attgatacca tgcaccaagc caatgaattt attgccttgg
    14161 acgatctctt ccgagcagca gcaatttatg ccgaagctat ttacgaattg atcaaataaa
    14221 acgatagaag tctgagatct tatgcttgga cttctttttg gagggaaagt agatgtctca
    14281 aatcgaaaga atcaaacagg ctatcatggc ggattcgcag aatgccagct atacagagcg
    14341 tggcattgag cctctctttg cagcgccaaa aactgctcgc atcaatatca tcggtcaggc
    14401 tccgggactt aaaactcaag aagcaggcct ttactggaaa gataaaagtg gtgaccgctt
    14461 gcgggactgg ctaggtgtgg atgaagatac cttttacaat tcaggttatt ttgctgtttt
    14521 gcctatggat ttctactttc caggacatgg caagtcgggt gatcttccgc ctcgtacagg
    14581 ttttgcagaa aaatggcatc cgcagctctt acaggaattg cctgatattc agttaaccct
    14641 cttgattggg caatatgccc aagcctacta tttacaggag aaaatcagtg ggaaggtaac
    14701 ggagagggtg aaacactata aagactatct gccagcctat tttccgctag ttcacccatc
    14761 accacgaaat caaatctgga tggccaaaaa tccttggttt gaggcagaag tagtgccaga
    14821 tttgaaaaaa agaattaaaa ccattttata gtcaatgaaa atcaaagagc aaactaggaa
    14881 gctagtcgta ggctgctcaa agtacagctt tgaagttgca gataaaactg acgaagtcgg
    14941 taacatacgc acggtaaggc gacgctgacg tggtttgaag agattttcga agagtattag
    15001 aagaaaaaga atgaaagaaa tagcctttga cgcattttac cagctttacc aaaacgacca
    15061 gctttcttta gtggatgtga gagaagtgga tgagtttgca gctcttcatt tagaaggtgc
    15121 ccacaaccta ccgcttagtc aattggctga tagttatgat taattggaca aagatcgctt
    15181 gcattatatt atttgcaaat ctggaatgag atcggcgcgt gcttgccaat tcctattaga
    15241 acaaggttat aatgttatca atgtccaggg tggcatgtta gcctttgaag aactttaaaa
    15301 ttttgcattt ctcctacttg gtgtggactg ggtaggagag ttttattttt agataattct
    15361 tatttttaag aaaattgaaa acatttaata tttgcctcgt gatgcttttt tcagactcct
    15421 aatcgtggta tactaggtca gtattttata aatatgaagg agatttttat ggctaaaaaa
    15481 ggtaccctaa caggtttgct cctgtttgga atattttttg gtgcggggaa cttgattttt
    15541 ccgccttctc taggtgctct atctggagaa cattttcttc ctgccatcgc aggttttgtc
    15601 ttttcaggcg ttggtatcgc cgtcttgacc cttattattg gaacgctaaa tcctaaagga
    15661 tatatctacg agatttcaac gaagatagcg ccttggtttg cgactcttta cctctcagtt
    15721 ctttacttgt caatcggtcc attctttgct accccacgta ctgctacaac agcttacgaa
    15781 gtagggatta gccccctttt gtcggatgca aataaaggac ttggcttgat tgtatttacg
    15841 gttctgtatt ttgcggcagc ctatttgatt tcgcttaatc catcaaaaat cttagaccgc
    15901 attggacgta ttttaacgcc agtctttgca attttgattg ttatcttggt cgttctggga
    15961 gctatcaaat atggtggaac aagtcctcaa gctgcttcag ctgcttatca agcttctgcc
    16021 tttggtacag gtttcctaga aggttacaat accttggacg cccttgcctc agtggccttt
    16081 agcgtaatcg cagttcaaac cttgaaacaa cttggatttt caagtaagaa agaatacatt
    16141 tcaactattt gggttattgg tatcgttgtt gcccttgcct tcagcgctct ttacatcggt
    16201 ttaggttttc ttggaaatca tttcccagta ccagctgaag cgatgaaggg tggaacacca
    16261 ggtgtttaca tcttgtcaca agccactcaa gaaatctttg gctcaacagc tcaactcttc
    16321 cttgcagcta tggttaccgt aacctgcttc acaacgactg ttggtttgat tgtgtcaaca
    16381 gctgagttct ttaatgagcg cttcccacaa atcagctaca aggtttatgc gacagccttt
    16441 accttgattg gatttgctat tgccaatttg ggtcttgatg cgattatcaa gtactcaatt
    16501 ccagtactgg ttatcttgta cccaatcacg attgctatcg ttatgattgt cattgtcaac
    16561 aaatttgttg ccctttcaaa accaggtatg cagttgacaa ttgctgtggt tacagttatt
    16621 gccattgcaa gcgtactagg aagctcgttt aaggttgagt ttcttgcaaa tcttgttagc
    16681 gttcttcctt ttgccaaggc atctctccca tggttggtgc cagccattgt tggaatcttg
    16741 ctctcattgg ttctaccaaa caagcaagaa agcgatgttt ttgaaatgga ataatcactt
    16801 aaatcacttt tgtagccaag tctacaggag tgattttctt tttttatccg atgataaatg
    16861 tgttataata ggtagcgaaa gaggtgaaga aatgaatcaa acagtagaat atatcaaaga
    16921 actgacagcc atcgcatcgc caacgggctt cactcgtgaa atttcgaatt atttagtcaa
    16981 gactctagaa ggttttggtt accagccagt tcgcacagcc aagggtggtg tcaatgtgac
    17041 catcaaaggt caaaatgatg agcaacatcg ctatgtgact gcccatgtgg acacgctggg
    17101 tgctattgtt cgtgctgtca aaccagatgg tcgtcttaaa ttggaccgta tcggtggttt
    17161 tccttggaac atgattgaag gtgaaaactg taccgttcat gtggctagca caggtcaaaa
    17221 ggtatcagga accatcctca tccaccaaac ttcttgccat gtctacaagg atgcaggaac
    17281 tgcagaacgc acgcaggaca atatggaagt gcgtttggat gtaaaagtaa gcaatgaaaa
    17341 agaaactcgt gctcttggga ttgaggtcgg tgattttatc agcttcgatc cacgaactgt
    17401 tgtgactgat acaggtttta tcaagtctcg tcatttggat gataaggtca gcgcagcgat
    17461 tttgctcaac ctgcttcgta tttataagaa agagaagatt gaattgccag taacaactca
    17521 ttttgctttt tcagtttttg aagaagtagg acatggtgct aattctaaca ttcctgctca
    17581 ggtagtcgaa tatctggctg ttgatatggg agctatgggc gatggccagc aaacagatga
    17641 gtacacagtg tctatctgtg tcaaggatgc ctcaggccct tatcactatg acttccgtca
    17701 acacttggtg accttggcga aagagcaaga tattcctttt aagctggaca tctatccatt
    17761 ttatggttcg gatgcttcag cggccatgtc cgcaggggca gaggtcaaac acgccctcct
    17821 tggcgcgggt atcgagtcta gtcattctta tgagcgtact catattgact cggtggtcgc
    17881 aacagagcgt atggttgatg cttacattaa gagcgcgttg gtagactaat atgtgtctta
    17941 tatgccagag aattgagctc atcaagaagg gagaaaatcc ctattttgtc aaagagttgg
    18001 aaacaggcta tcttgtgatt ggagatcacc agtattttgc aggctatagt ctctttctag
    18061 ccaaggaaca cgtcacggaa ttgcaccatt tgaaaaagga aacaagactc cgttttctcg
    18121 aagaaatgag tctagtccaa gaggcagttg ccaaggcatt tgctgctgag aaaatgaata
    18181 tcgaacttct agggaatggc gacgcccatc ttcattggca tctgtttcct agacgaagag
    18241 gtgatatgaa gggtcacggt ctcaatggac gtggtccagt ctggtgggtt ccctttgaag
    18301 aaatgacagc agaaacctgc caagtaaaac cggatgagat tgaaagatta gttaaacgtt
    18361 tattgataga agtagataaa ctattaaaat aaaggagtag aaatgaaaaa aagatacctt
    18421 gtcttgacag ccttgctagc cttgagtcta gcagcttgtt cacaagaaaa agcaaaaaat
    18481 gaagacggag aagctaagac agaacagaca gccaaagctg atggaacagt aggaagtaag
    18541 tctcaaggaa ctgcccagaa gagagcagaa gtggttaaca aaggagacta ctacagcatt
    18601 caaggcaaat acgatgaaat catcgtagcc aataaacact atccattgtc taaagactat
    18661 aatccagggg aaaatccaac agccaaggca gagttggtca aactcatcaa ggcgatgcaa
    18721 gaggcaggtt tcccaatcag tgaccattac agtggcttta gaagttatga aactcagacc
    18781 aagctctatc aagattatgt caatcaagat gggaaagaag ctgccgaccg ttactctgct
    18841 cgacctggct acagcgaaca ccaaacaggc ttggcctttg atgtgattgg gactgatggt
    18901 gatttggtaa cagaagaaaa agcagcccaa tggcttttgg accatgcggc tgattatggc
    18961 tttgttgtcc gttatctcaa aggcaaggaa aaggaaacag gctatatggc tgaggaatgg
    19021 catcttcgtt atgtcggaaa agaagctaaa gaaattgctg agagtggtct cagtttggaa
    19081 gaatactatg gctttgaagg cggagattac gtcgattaaa aaataaactt ttctcttgca
    19141 attccagata aatagtgtat aatagatggg tatgtgaaaa gcatacttgt gggaggtaaa
    19201 aatctctaat taccgccaaa accacaaagg aggatttaaa aatggctaaa aaagtcgaaa
    19261 aacttgtaaa attgcaaatc cctgctggta aagctacacc agctccaccg gttggacctg
    19321 ctcttggtca agctggtatc aacatcatgg gattcacaaa agagttcaac gctcgtacag
    19381 ctgaccaagc tggtatgatc attccagttg ttatctcagt ttacgaagat aaatcattta
    19441 ctttcgttac gaaaacacca ccagctgctg ttcttttgaa aaaagctgca ggtgttgaaa
    19501 aaggatcagg tacacctaat aaaactaaag ttgctacagt tactcgtgca caagtacaag
    19561 aaattgcaga aactaagatg ccagatttga acgcagcaaa cgtagagtct gcaatgcgta
    19621 tgatcgaagg tactgctcgt tctatgggat tcactgttgt tgactaatca ataacacagt
    19681 agaaaatctc acagcagtct attagttgct tttcatacta ggcgagtgac tgaagcttgt
    19741 acttgggtac agcaagggag cttaaggccg taggaagaga aaaatagtag actgaaaacc
    19801 cgcaagactt catcatttcg agaagtgacg tgggagatga aaatcgattg aaccacttac
    19861 aaggagaata gaaaatggct aaaaaaagca aacaacttcg tgctgctctt gagaaaatcg
    19921 acagcacaaa agcatacagc gtagaagaag ctgtagctct tgcaaaagaa actaactttg
    19981 caaaatttga cgcaactgta gaagttgctt ataacttgaa catcgacgtt aaaaaagctg
    20041 accaacaaat ccgtggagca atggtattgc caaacggtac tggtaaaact tcacgtgttc
    20101 ttgttttcgc acgtggtgca aaagctgaag aagcaaaagc tgctggtgca gactttgttg
    20161 gtgaagatga ccttgttgct aaaatcaacg acggttggtt ggacttcgac gtagttatcg
    20221 ctacacctga tatgatggct cttgttggac gtcttggacg tgtccttgga ccacgtaact
    20281 tgatgccaaa ccctaaaact ggtactgtaa caatggatgt tgctaaagca gttgaagagt
    20341 ctaaaggtgg taaaatcact taccgtgctg accgtgcagg taacgttcaa gcaatcatcg
    20401 gtaaagtatc atttgaagct gaaaaattgg ttgaaaactt taaagctttc aacgaaacaa
    20461 tccaaaaagc aaaaccagct acagctaaag gaacttacgt aacaaacttg actatcacaa
    20521 ctactcaagg tgttggtatc aaagttgacg taaactcact ttaatcagta gttaaagtaa
    20581 tgtaaaaaag ttgaagacgc tatgtctcaa ctttttttga tatacgacgg gcatgttgta
    20641 tagtagattg aaactagaat agtacacatc tacttctaaa acattgttag aaattgattt
    20701 gactgtcctg atcgattcgt cctgttctta tttcatttta ttatatatct gaggtgtaag
    20761 tcctcggtgg gcacctgcta ccggtgaacc caatagcgat tcccaagtct gattatcgtg
    20821 aggtagcgga ttaaaacggt ctggggatag accgttttaa gtctgacgct agaaataaga
    20881 atcgtcagag gaagggatag cgaaatcgtt gctctacgaa caggaacgtg ataataaggc
    20941 ctatatagcg gataaggtat ctggctgggg cgaccactat caattagtag ctcagaagtc
    21001 ggtacttaaa cgtgctatat caaaaccagt ccttgaaaaa cgtggactgg tttcgtgttt
    21061 ggattattac cttgaacgac atgcgttaaa agttagttga accgccgtat gccgaacggc
    21121 acgtacggtg gtgtgagagg ggctagagat tatcccc
//
LOCUS       DAVKJD010000029        20901 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKJD010000029 DAVKJD010000000
VERSION     DAVKJD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.029"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..511)
                     /locus_tag="VW157_001093"
     CDS             complement(<1..511)
                     /locus_tag="VW157_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9552407.1"
                     /translation="MITKQSAFLHNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESF"
     gene            679..2001
                     /locus_tag="VW157_001094"
     CDS             679..2001
                     /locus_tag="VW157_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9552408.1"
                     /translation="MNNILTFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGE"
     gene            2003..3889
                     /locus_tag="VW157_001095"
     CDS             2003..3889
                     /locus_tag="VW157_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU9552409.1"
                     /translation="MVIRVFDQQKNAYSSFALEELSYYMNRVFKTDIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKELSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRTLTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPAPKPYMRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWKNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFRL"
     gene            4057..4401
                     /locus_tag="VW157_001096"
     CDS             4057..4401
                     /locus_tag="VW157_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9552410.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLHDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            4411..5823
                     /gene="lacG"
                     /locus_tag="VW157_001097"
     CDS             4411..5823
                     /gene="lacG"
                     /locus_tag="VW157_001097"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9552411.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGINMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVQYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKVGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGRSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            5893..7572
                     /locus_tag="VW157_001098"
     CDS             5893..7572
                     /locus_tag="VW157_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9552412.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGIIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QLISFVFLSLMLVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPGEIDI
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
     gene            complement(7698..9137)
                     /locus_tag="VW157_001099"
     CDS             complement(7698..9137)
                     /locus_tag="VW157_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9552413.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMAFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMTIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLSLIFIVSLDSNDFLIVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(9141..10490)
                     /gene="trkA"
                     /locus_tag="VW157_001100"
     CDS             complement(9141..10490)
                     /gene="trkA"
                     /locus_tag="VW157_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9552414.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            10679..11527
                     /locus_tag="VW157_001101"
     CDS             10679..11527
                     /locus_tag="VW157_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9552415.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            11542..12090
                     /locus_tag="VW157_001102"
     CDS             11542..12090
                     /locus_tag="VW157_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9552416.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            12097..12354
                     /locus_tag="VW157_001103"
     CDS             12097..12354
                     /locus_tag="VW157_001103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552417.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            12456..14138
                     /locus_tag="VW157_001104"
     CDS             12456..14138
                     /locus_tag="VW157_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552418.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLQTELVKETPELQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            14123..14953
                     /locus_tag="VW157_001105"
     CDS             14123..14953
                     /locus_tag="VW157_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9552419.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLAILFLVVSLLLILQNQGRFYNPWK
                     "
     gene            15146..15649
                     /locus_tag="VW157_001106"
     CDS             15146..15649
                     /locus_tag="VW157_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9552420.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      15703..15937
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            16102..16665
                     /locus_tag="VW157_001107"
     CDS             16102..16665
                     /locus_tag="VW157_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9552421.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TPLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     gene            16679..17305
                     /locus_tag="VW157_001108"
     CDS             16679..17305
                     /locus_tag="VW157_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9552422.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPDILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            17333..17749
                     /locus_tag="VW157_001109"
     CDS             17333..17749
                     /locus_tag="VW157_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552423.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQTLLADRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFFGLKSRRRENGFQLGILHYLRIISPL"
     gene            17647..17958
                     /locus_tag="VW157_001110"
                     /pseudo
     CDS             17647..17958
                     /locus_tag="VW157_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            18086..18673
                     /locus_tag="VW157_001111"
     CDS             18086..18673
                     /locus_tag="VW157_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9552424.1"
                     /translation="MKLEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVTPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            19063..20670
                     /locus_tag="VW157_001112"
     CDS             19063..20670
                     /locus_tag="VW157_001112"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9552425.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAAAMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
BASE COUNT         6211 a         3597 c         4616 g         6477 t
ORIGIN      
        1 taaaagattc ccatatgggg ttgtttgtaa tgatacttcc ggagtctttg ttataatgtc
       61 caggtatcga taagccaaat ccaagtaatt ttgtctccaa agggacgtac tctatagctg
      121 tttttatcat attggtaatg gtggaattga tttctgaaat aggataatct tcattcaaaa
      181 tttctgtctc gaaaagagtt ttcccttgta agtcacatag actgagaaag atacctagga
      241 cgtttatttc aatacctcca acaacatatg gaatattatc tgtgatagaa atagtttttc
      301 tacgtcttcc agatccggaa aactcgctaa attcatcacc agtttcgtat atgagggact
      361 gttcacttag ctcttttatg atgttagtcg ttgttgctgg agtaagtcct gtttcattaa
      421 caatatctgt acgggatgtt gctggatttc gatatagaaa ctccagtata gtggctctat
      481 tgtgtaaaaa ggctgattgt tttgtaatca tttgtttcct cctttggttt attttatatt
      541 aaacataaga aaaaatcaat taaaattatt attctataca aagaaaaaaa tattgtaaaa
      601 acagcaaaaa atatattgac aacgctttct gttagtggta tatttaattt ataaaaaata
      661 aaaaaaggag ttccggtgat gaacaatatt ttaacgtttt tagaaacgaa agtcgctccg
      721 tttggtgaaa aagttggcaa ccaacggcat ttgaaagcta ttcgtgaagg atttatgatg
      781 gcaatgcctt tgattttagt cggctcttta tttcttattc taatcagttg gcctcaagag
      841 gcttttacaa attggctgaa tagtgttgga ttgctaagta tcttgacaac tatgaatcag
      901 tcaacagtag cgattatttc cttggtcgct tgtttcggta ttgcctacag gttgtcggaa
      961 ggatatggta cagatggtcc gtctgcaggg attatagcct tatccagttt tgtattgatg
     1021 gcacctcgtt tttcaagtat ggtttatgat aaaaatgggg agcaggtcaa gcagttattt
     1081 ggcggcgcaa taccattttc tagcctgaat gcatcttcgt tgtttatggc gattactatt
     1141 ggattggtta cagcggagat ttatcgtatg tttatccagc gcggaattac gataaaaatg
     1201 ccaagtggtg tcccagatgt ggtcagtaaa tcattttcag ctcttttacc tggttttact
     1261 acttttgttt tgtgggctct ggtcttaaaa ggtctggaag cagcaggagt tgcaggaggt
     1321 ctcaacggac ttctaggtgc aattgttgga acaccgctta agttaattgc aggaacgctt
     1381 ccaggtatga ttctatgtgt tattgtaaac tcattctttt ggttctgtgg agttaatggg
     1441 ggacaagttt taaatgcttt tgtagaccca gtttggttac aatttactac agaaaaccaa
     1501 gaagctgtgg ctgcaggaca aaccctacaa cacattatta cattaccgtt taaagattta
     1561 tttgtattta ttggtggcgg tggagcgact attggtcttg cgatttgtct cttcctattt
     1621 agtaagagtc gtgcgaataa aacattaggt aagctagcta ttataccgtc tatctttaat
     1681 atcaatacag ctattctatt tacgtttcca acggttttaa atccgattat gctgattccg
     1741 tttattgcta ctcctacaat caatgcctta attacctatg tatcaatggc tgtaggatta
     1801 gtaccttata caacaggtgt aattcttccg tggacaatgc caccgatcat aggaggcttc
     1861 cttgcgacag gggctagttg gcgaggagct ctattacaag ttgttttaat tttggtttct
     1921 gtggcaattt attatccatt cttcaaaatt gcagataaac gcaatcttga aaaagaaaaa
     1981 gctactgttg gaggggaata agatggttat cagagtattt gatcagcaga aaaatgctta
     2041 ttctagcttt gccttagagg aattaagtta ctatatgaat cgggtcttta agactgacat
     2101 agaacttgtc gaggagaagg aagcggatat ttttgtagga ttagtcaata aagaggatag
     2161 aaaagaccat gttcttatct cattagacaa gggtaagggg agaattgagt ctaatacaat
     2221 tgtaggttta cttattggaa tttaccgaat gtttcatgaa tttggggttg tgtatactag
     2281 accagggcgc ggacatgact ttgttccaga gttacgattt gaagattttt tagataaaga
     2341 gctatctata gatgaaacag ccagttacta tcatagggga gtatgtatag agggagcgga
     2401 ttcatttgaa aatatactag atttcattga ttggctacct aagattggga tgaacagttt
     2461 tttcatccag tttgaaaatc cttactcttt tttgaaacgt tggtatgaac atgaatttaa
     2521 tccatatcta aataaagaaa aattctcaaa tgaattagta caagaattga gtgataggtt
     2581 ggataaagaa ttgcaaaaaa gaggtcttat tcatcatcgt gttggtcatg gatggacagg
     2641 tgaggtttta ggttactctt caaaatttgg ctgggaatca ggtcttagta tttcagagga
     2701 gaagaaaccc tatgtcgctg aaataaacgg gaaacgagaa ttgtttaata cggctccgat
     2761 tttaaccagc ctggattttt caaatccaga tgtggcggat aagatggtag aaattatcaa
     2821 ggattatgcc aagaaaagac ctgatgtgaa ttacttacat gtatggttgt cggatgctcg
     2881 taataatatt tgtgaatgcg aaaactgtag acaagaatta gtttcggatc agtatattcg
     2941 tattctcaat caattggata ggactttaac gagtgagggg ttagatacaa agatttgttt
     3001 cctactttat catgagttgt tatgggcgcc tcagaaagaa aaattagata atcctgaacg
     3061 ctttaccatg atgtttgcac cgattacaag aacatttgaa atgagttatg cagatgtaga
     3121 ttttgacaat tccatacctg ctcctaaacc ttatatgcgt aataaaatta tacttccgaa
     3181 ttctcttgag gaaaatttat cttatctttt tgagtggcaa aaaacattta aaggagatag
     3241 tttcgtatat gactatcctt tagggcgtgc tcattatggc gatttaggct atatgaaaat
     3301 tagtcaaact atttacagag atgtatctta tctttcaaac ctacatttga acgggtacat
     3361 ttcgtgtcaa gaattacgtg ccggattccc tcataatttt cctaattatg tcatggggca
     3421 aatgctctgg aagaagacaa gaagttatga agaattgatt gaagaatact tttctgcttt
     3481 gtatggggaa aattggcagt ctgttgttga atatttagaa aaattatcta gttattcctc
     3541 atgtgattat tttaatgcaa ttggcagtcg tcaaaatgat gttttagcga atcattatta
     3601 tatagcttac aatctagctg ataatttttt accaattatt gaggaaaata tttctaagtt
     3661 attaaatagt caaaaggatg aatggaaaca gctcagttat catcgtgaat atgttgttaa
     3721 gatggcgaag gctttatatc ttcaagcaac tggaaaaaca aggcaagctc aagatgagtg
     3781 gaaaaatgtt ttgaattata tccgtgggca cgaattacta ttccaatcta atttggatgt
     3841 ttatcgtgta attgaagtag caaaaaatta cgctggtttc cgcttataaa tcataagtat
     3901 agaaaatgaa ctaaggtatt cagggaagat tgatcctaaa tattatgaaa tttaaggatt
     3961 tttaagatat ttagggtcga ctttctattt gtatcgtagc gaagtcattt taataatgat
     4021 gtgtaaaaga ttgatcaaga ttgaggagga agaaagatga aatcaaaaga agaaataaat
     4081 atgcttggtt ttacaattgt cgcttacgca ggagatgcaa ggtcagattt gatggatgct
     4141 ttggcgtttg cgagagatgg atattttgaa caggcaagag aattggttga gtctgcaaac
     4201 gactcaatag tgtctgccca tcgagaacag actaatttat tagcggagga ggcatctgga
     4261 gataattttg aagtgagttt tatcatgatt catggtcaag atactttgat gacaacgatg
     4321 ctattgcatg atcaggtaaa gttttttatt gatgaatatg aacgaattcg aaagattgaa
     4381 gaacatattg gtttgcaatg aggattagtc atggaaaatt tacaggttaa agccttaccg
     4441 aaggagttct tattaggagc tgctaccgct gcttatcaag tagagggtgc aactagggta
     4501 gatggcaaag gaataaatat gtgggatgtt tatttgcaag aaaatagtcc gttcttacca
     4561 gatccagcta gtgattttta ttatcgttac gaagaggata tagctttggc ggcagaacat
     4621 ggtttgcagg ctttgcgttt atctatttct tgggttcgta tatttcctga tatagatggg
     4681 gagcctaata tattggctgt tcagtattac catagagtat ttcagtcctg cttaaaacat
     4741 aatgtgattc cgtttgtttc tttacatcat tttgattcgc ctcagaaaat gttagaaaca
     4801 ggggattggt tgaatagaga gaatattgat cgtttcatac gatatgctcg tttttgtttc
     4861 caagaattta cagaagtcaa gcattggttt acaatcaatg aactgatgtc tcttgctgca
     4921 ggtcaatata taggaggtca gtttcctcca aatcaccatt ttcaattatc tgaagcaatt
     4981 caagcgaatc ataatatgtt gttggcgcat gctcttgcag tactcgaatt tcatcaatta
     5041 gggattgagg gaaaggtagg ttgtattcat gctttaaagc caggctatcc tattgatggg
     5101 caaaaagaaa atattttggc agctaaacgg tatgatgttt ataataataa atttctatta
     5161 gatggaactt ttttgggcta ctacagtgat gacacgcttt ttcacttgaa tcaaatattg
     5221 gaagctaata attctagctt tattattgaa gatggtgatt tagaaattat gaaaagagct
     5281 gcacctctta atacgatgtt tgggatgaat tattatcgtt cagaatttat tcgtgaatac
     5341 aaaggtgaaa atagacaaga atttaattca acaggaataa aaggacggtc ttcttttaaa
     5401 ttaaatgctc taggtgaatt tgtaaaaaaa cctggtattc cgacaacaga ttgggattgg
     5461 aatatttatc ctcaagggtt atttgatatg ttgcttcgta tcaaagaaga atatcctcaa
     5521 catccggtca tttatttaac tgaaaatggt acagccctta aagaagttaa gccagagggc
     5581 gagaatgata ttattgatga cagtaagaga atccgttata ttgagcaaca tttacacaaa
     5641 gttttagagg ctcgagatag aggagtcaat attcaaggct attttatatg gtctttgcaa
     5701 gatcaatttt cttgggcgaa tggctacaat aagcgatatg gtcttttctt tgttgattat
     5761 gaaacacaga agagatatat taagaaaagt gctctttggg taaaagggct aaaacggaat
     5821 taaggttagc gatttgactg atgtttaata tattttaact atgagcttaa attttttaca
     5881 ggaggagttt ttatggataa gttagtcgct gccattgaaa agcaacaagg gaaatttgaa
     5941 aaaatttcta ctaataatta tatgatggct attaaagatg gatttattgc tactatgcct
     6001 ttaattatgt tttcaagctt tttgatgatt attattatga ttcctaaaaa tttcggagta
     6061 gagttaccga gtccagctat tgcctggatg aggaaagtgt atatgctaac catgggagtt
     6121 ttgggtatta ttgtttcagg gacagttgca aagtcattgg ctggaaatgt taatagaaaa
     6181 atgcctcctg gaaaggtaat aaatgatact tctgcaatgt tggcggccat atgtggctat
     6241 cttgtattaa ctgtaacgct tgtagttgat gagaaaacgg gatctacaag tttgtcgaca
     6301 aactatttag gatctcaagg attgataact tcgtttgtca gtgcctttat tactgtaaat
     6361 gtttaccgat tctgtattaa gcgagacatt actattcatt tacctaagga agttcctggg
     6421 gctatatcac aagcttttag agatattttt cctttttctt ttgttttact tattagtggt
     6481 ttgttagata ttgtatctcg gtttagttta gatgttcctt ttgcccaagt atttcaacaa
     6541 ctattgactc ctatttttaa gggggcagaa tcatatcctg ctatgatgtt gatttggttt
     6601 atgtgtgctt tgctttggtt tgttggaatt catggaccat ctattgtctt acctgctgtt
     6661 acagctttac aactgagcaa tatggaagag aatgctcaac ttcttgcaaa tgggcagttc
     6721 ccttatcatt ctttaacacc taatttcggg aattatatcg ctgctattgg aggaacgggg
     6781 gctacctttg ttgtaccatt tattttgatt ttctttatgc ggtctaaaca attaaaatcg
     6841 gtaggtaaag ctacaattac tcctgtttta tttgcggtaa atgaacctct tctatttggt
     6901 atgcctgtta ttttgaatcc ctatcttttt gtcccttttt tgatgactcc accagtgaat
     6961 gtatttctag gaaaggtatt tattgatttc tttggaatga atggatttta tatccagtta
     7021 ccttgggcct ttcctggtcc cttgggattg ttaattggaa cgaattttca acttatctcc
     7081 tttgtatttt tatctttgat gttagttgtc gacatattga tttatttgcc attctgtaga
     7141 gcgtatgata gacagttatt ggtgaaagaa gatattgcaa gctcaaatga tattatttta
     7201 gaggaggata caagtgaaat aatgcctggt gagatagata taataaaaag taaggagttg
     7261 aaagtactgg ttctttgtgc agggtctgga acaagtgcgc aattagccaa tgcaattaac
     7321 gagggcgctc aattagcaga agttagagtg attgctaatt caggagcgta cggagctcat
     7381 tatgatatta tgggtgttta tgatttaatt attctggccc cacaagttcg gagttattat
     7441 agagagatga aggtggatgc agaaagattg ggtattcaga tagttgctac cagaggaatg
     7501 gaatatattc atttaacaaa gaatccaagt aaagccttac aatttgtatt ggagcattac
     7561 caagctgtgt agtaagtttt tccatctttt atttgagtaa ggactttgct tacagacagg
     7621 cttggattta aaaacgttcc ccctttttta atataagaat ccctctttca caattgtaaa
     7681 aagagggatt ttgtatttta tctcttagac caagttcttt tcataaagag aaggaggatt
     7741 gggtaaatct ccaagcgccc tgcaatcatt gcaaaggata ggagaatttt tgagatggga
     7801 ctaaagattg agaaactaga agtggttcct agaataggcc cgatattatt gaaacagcta
     7861 aagacagcgc tggtcacgat cagaaaatca ttgctatcta ggctgacaat aaagataagg
     7921 gatagcaaaa tcatagcata gatgacaaag tacttgagaa ttttatgctg ggtatcctta
     7981 tcaatcaccg ttttattcac atggagagtc aaaacacggt ggggagatag aatggacaaa
     8041 atttgatttt tggcaatttt tgaaaggatg aggcctcgaa taatcttgag tccacctgca
     8101 gttgatccag cagaaccacc gattgtcatg aggaagagga ggataaactg ggagaagagg
     8161 ggccagttgg taatatctcc atatccaaaa ccagttgttg taatgatgtt ggaaacctgg
     8221 aagaaggcca tttcaaagct tttggaaaat cctgggtaga ggtagagggt gttgaggcta
     8281 atcaagcctg tagaaaccag cacgatgacc aagtaagccc gaagctcttc atctccaaag
     8341 aaggccttaa cccgacggag cataaggtag tagtagagat tgaaatttac cccaaaaacc
     8401 aaaactccta tactaaccag ataggtaatc agtgagctac catagtgggc aattccatcg
     8461 ttatagacgg taaatcctcc agttcccgct gtccccatag caatgacaaa actatcgtag
     8521 agaggcatac cggctagata atagatgatg acaaagaggg aaaagagggc tatgtaaagg
     8581 agatagagga tctgggcagt gttttttaat ttggatacaa ccttaccaaa gacaggacca
     8641 ggaacctcag ccttcattac ttctaggtga ctatttttgg cattgtccat aatagcaagt
     8701 gcaaaaacaa gcactcccat ccctccaatc aagtgggtaa aacttcgcca gaagaggagg
     8761 gaacggctga gaaccgaaat gtcgttcaaa atacttgctc cagtagttgt aaacccagaa
     8821 ctaatttcaa aaaaggcatc aataacgcta gggatttgcc cagaaaagac aaaggggaga
     8881 ccaccaaaga aagaccaaag gatccaacag agggcaacga tcaagactcc ctccttggca
     8941 taaatccgtt gattttttgg cttctgtaaa ctccctgaac cgcctaacaa tacgagaatc
     9001 cctatggtcg aaaagagggc tgtaaagact tggctcgatt cacggtaata gacagcgata
     9061 gccacaggaa ccaagaggag aacagcttca atcaaaagta actttgaaag gaggtaacga
     9121 atcatacttt tattcatttc ttacctcgca atcaagtcat aaatcttggt gatgtttggc
     9181 aacaaggttg ttactaggag cttgtctcca acttccaaca tatcctcccc agttgggaaa
     9241 atagtcttgc cctttcgaat aatggctgca ataagaaccc cttttttcaa tttcagttga
     9301 gaaagaggtt tggcagtcat tttattggct tccttgatat ggaattgcag ggtttcaatt
     9361 tggccattgg ctagatggtg catggcttga aggtctgaat actgggcatt aactcgacca
     9421 cgaataaagt gcataatcgt atctacagcg atgcttttag gtgtgatgat acttgaaaaa
     9481 tcaggcgcat tgataatctc gaggagactg gtacgattga ccttagtaat atttttctgt
     9541 acacctaccc tgtcaaggaa catagatgta atcagatttt cctcatcgac tcctgttaga
     9601 gtcgcaacgg catcatagtg ttgagcactt tcttccagca ggatatcttt tgcggttcca
     9661 tctccttgaa cgatgtagag atttgggaat ttctcgctaa agaagctggc gatttcagga
     9721 ttgatttcaa tgacttttgt atcgatacga ctatctttga gaataccaag tagataatag
     9781 gcaattctac ctgccccaac gatgagaagg ctcttcacgg cgcgtgattt aaaataatta
     9841 tggaagagta tcatatcgac acggttacca gtgacaaaga ttctatcttt atcctgtaca
     9901 gtcatgtcac cgcttggaat gataatttga tgatccctct ctatcgcaca gacaatgaca
     9961 ttaccaaatt ttttacgaaa atcagaaatg ggcatttggc aaagaccgct ggtggacttg
    10021 acgacaaatt ccatgaggct aacgcgtcca ccagcaaagc gttcgacaga cagggcgttg
    10081 gggaagtcaa tgatattcgc gatagcgcgg gcagccaaga gctcaggatt aacgataaga
    10141 gaaaaaccga gaatattctt ttccttgaaa taagagttag aatattcagg gttccgcacc
    10201 cgaacgatag tttctttagc tcccattttc ttggctagaa ctgctgcaat catgttgact
    10261 tcatcgtgct cagtcagggc gataaagata tcacaatctt ggacgctggc ttgctcaaga
    10321 atggcaaaat cggccccgtt accaaggata ccaatgatat caaagcgact gacaatatga
    10381 ttgagaacag cttcgtcttg ctcaatcagc aaaacatcat gcttttctgc aaccaaggag
    10441 cgacagaggg caaaaccaac ttttccccct ccgacaagga taattttcat aataaaacct
    10501 actttttcat gatgtaacta tcataccctt tttcaagaaa aaatgcacct actagctaat
    10561 aaccagagtt tttagtagga aatttgctat aaggtaaaac tataccctaa ccaattgaaa
    10621 tagctattag cgactttctc tgaaatatgg tatgataaag gatatacaag gagataaaat
    10681 gaataataat ttactggtat tacaatcaga ctttggtctg gttgatggtg cggtatcggc
    10741 tatgattgga gtggctttag aagagtctcc aaccttaaaa atccatcact tgacgcacga
    10801 tatcacgcct tataatattt ttgaggggag ctatcgtctc tttcagacgg tggattactg
    10861 gcctgaggga acgacgtttg tatcggttgt cgatccaggt gtcggttcga aacgtaagag
    10921 tgtagttgcc aagactgcaa aaaatcaata cattgtcacg ccagataatg ggacgctttc
    10981 ctttatcaag aaacacgttg gcattgtagc cattcgtgag atttctgagg tggccaatag
    11041 gcgtcaaaac acagagcatt cttatacctt ccacggtcgt gatgtctatg cctatactgg
    11101 tgctaaactg gccagtggtc acattacttt tgaggaagta gggccagagc tcagtgtgga
    11161 acagattgta gagcttccag tcgtagcgac catcatagaa gatcatctgg tgaagggagc
    11221 cattgatatt ctggatgtgc gtttcggttc gctttggacc tctatcacac gggaagaatt
    11281 ttacaagctg gaaccagaat ttggtgatcg ttttgaagtg accatctatc atgctgatat
    11341 gctggtctat caaaatcagg ttgtctatgg caaatcattt gcagatgtga gaattgggca
    11401 acccatcctt tacatcaact ctctctatcg tttaggtttg gctatcaacc aaggttcctt
    11461 tgccaaggcc tataatgtag gtgtcggttc atcttggacc attgaaataa agaaaataga
    11521 aggataaaat aggagaatag tatggaaatc aaatttacaa ttaaacaagt tgttgctgtc
    11581 ggaattggcg ctgccctctt tgtcgtcatc gggatgatca acattccaac ccctgttcca
    11641 aatacaagca tccagcttca gtatgcggta caagcgctac tttctattat ttttggaccg
    11701 attatcggtt tgcttgtcgg gttgattggt catgcaatca aggactctct tgctggttat
    11761 ggtctatggt ggacttggat tatcgctagc ggactctttg gtctagttgt gggacttttt
    11821 agaaagtacg ttcgagtgat caatggtgtt tttgactgga aagatattct tatttttaac
    11881 ctcattcaac tacttgcaaa tgcccttgtt tggggtgtct tggcaccact tggagatgtt
    11941 gtgatttatc aagaagcggc agaaaaagta tttgcacaag ggattgttgc gggaattgcc
    12001 aatggtgtat ctgtagctat tgcaggaact cttctcttac ttgcctatgc aggaacccaa
    12061 actcgtgcag gaagtttgaa aaaggactaa tactcaatga aaatcaaaga gcaaactagg
    12121 aagctagccg caggttgctc aaaacagtgt tttgagattg tggatagaac tgacgaagtc
    12181 agctcaaaac atggttttga ggttgtagat gaaactgacg aagtcagtaa ccatacctac
    12241 ggcaaggcga agctgacgtg gtttgaagag attttcgaag agtataaaat gatggggaag
    12301 gctgggcaac tagtcttttt cgatgtttat agattagtta ggcaagtaag ctaagatagg
    12361 aatctttttg gcgctaagat tttaagagaa gagactgcaa gaagggcttg atttatgata
    12421 aaatagaagt ctaagaagcg aatgaagaga gtaagatgaa agaagctata attgagtgga
    12481 aggatttctc tttccggtat gaaacacaac aagaaccgac cttgcaaggg atagacttga
    12541 ccatttacaa gggagagaaa gtcttaattg ttggaccatc tggatcaggt aaatctacct
    12601 tgggtcagtg tttgaatggg attattccca atatttacaa gggtcagaca tatggagaat
    12661 ttttgataaa gggtcaaaca gcctttgata tgagcatcta tgataagtct catctggtta
    12721 gcacagtttt gcaggataca gatgggcagt ttattggctt gtctgtggca gaagatttgg
    12781 cgtttgctct ggaaaatgat gtgacagccc tagatgagat gaaaggtcgt gtttataaat
    12841 gggctgaaaa gctggacctt cttcctttac tggatcagcg tcctcaggat ttgtcaggtg
    12901 gacaaaagca gcgagtcagt ctggctggtg tcttgattga tgaaagtccg attctcttgt
    12961 ttgatgagcc actcgccaat ctagatccca agtcaggtca ggatattatc gaattgattg
    13021 accagattca taaggaagag gggacgacga ctctgattat cgagcaccgt ttggaggacg
    13081 ttctgcatcg ccctgtggat cggattgtct tgataaacga tggtcgtatc ctttttaatg
    13141 ggagccctga ccagttgctt gcgactgatt tattgactca aaatggaatt cgagaacccc
    13201 tttatctaac gactctccgt caattaggtg tggacttagt caaggaagaa caattagcag
    13261 atctggataa cttgtctatc tcaaaagggc aagtccagtt acagacggaa ctggtaaaag
    13321 aaaccccaga attgcagtca ctctttagac tagaggaagt atctttttct tatgatgata
    13381 gaccgatttt aaaatcccta catttagata ttaaaaaggg tgaaaagatt gctattgtcg
    13441 gaaaaaatgg agcagggaaa tcaactctag ccaaggctat aagtagcttt attcagacgg
    13501 aaggacgcta tctttgggaa aaacaggata taaaaggcga ttctgttgca gagcgggcgg
    13561 aacgagtagg ctatgtgcta caaaatccta atcaaatgat ttcaaccaat atgatttttg
    13621 atgaggtggc tctagggctc cgtttgcgag gtgtggatga gaaggaaatt gaaacgagag
    13681 tctatgaaac cttgaaaatc tgtggacttt atgaattccg taattggcct atttctgccc
    13741 tgtcatttgg tcagaaaaaa cgtgtcacca ttgcttcaat tttggtctta ggagctgaaa
    13801 ttattctcct agatgaaccg actgcaggtc aagatcagaa gaactatact gagattatgg
    13861 aatttctcga agagttacat caaaaagggc ataccattgt catgattacc catgatatgc
    13921 aattgatgct ggattattca gaccgggtcc ttgtcatggt ggatggagaa ttgattgccg
    13981 atactgttcc agccagtctg ttgagcgatc ctgagctgtt agtaaaagcc aatctaaaag
    14041 aaacctccat ctttaacttg gctaagaaac tagatgtgga tccactggat ttaacggcat
    14101 tttacaaaga aaggagagag ggatgcaagc taaattaatc ggttaccagc atagagatac
    14161 tgtgattcat cgcttgtcag gagctgggaa acttctcttt ttcattctgg tatcattggc
    14221 ggccatgatt agctatgata ccagaatgct cttgctgatt gccatctttt cagtctttct
    14281 cctctattta tcagaaatcc gctttaaaga tgtctccttt gtagccgttt ttgcgacggt
    14341 atttgccgtt ttaaacgttt tgatggtcta tctcttttct cccgagtatg gggttggact
    14401 ttatggagag agaagtgtga tttggcaggg aatcggtgtc tacactctaa ccagccagga
    14461 gctcttttat ctgctaaatt tggccattaa gtatctttgc accattcctc tggctattat
    14521 ctttttgatg acaacccacc ctagtcagtt tgcttccagt ttaaatcaaa ttggtgtgcc
    14581 ttacaagatt gcctattctg tcagtctaac cttgcgctat attccagatt tgcaggaaga
    14641 attctttacc atcaagatgt ctcaggaagc gcgtgggatg gaattatcca agaaagcttc
    14701 tcttatgcaa cgaatcaaag gcaatctgct cattattacg cccttgattt ttagctcgct
    14761 agaacgcatt gataccattg cgactgccat ggagcttcgc cgctttggga aagagaaaaa
    14821 acgcacatgg tatagttatc aggccttgaa aaaaggagac tatattacct tgctcttggc
    14881 aatcttgttt ttagtagtta gtttactact tatcttgcag aatcagggac gattttacaa
    14941 cccttggaaa tagcttgaaa aaattgaaaa aatcaagtca tttctattga caatgattct
    15001 gaaagtgtta tactaagaaa gtagtttcgc tgatttactt caaacctgtt gtgaggtaag
    15061 ttaacgatgc cttaaccacg ctgtttgctg agcttgactc cgggcagtgt ggctattttt
    15121 ttgcaatggt gaaaggaagc aagtcatgac aaatcacatt gtattatttg aacctcaaat
    15181 tccacaaaat acgggcaata tcgcgcgtac ttgcgctgcg accaattctc ccctccacat
    15241 catcaagcct atgggctttc ctattgatga ccgcaagatg aagcgggctg gattggatta
    15301 ctgggataaa ctagagattt atttttacga gagtttggaa gatttcatat ctcagatgaa
    15361 gggaaaactc tatctgattt cgaaattcgc ggaaaaagtc tattctgagg tggatttatc
    15421 gactgacgag gaccattatt ttctctttgg acgtgaagac aagggcttac ctgaagattt
    15481 tatgcgagaa catcctgaga aggctctccg tattcccatg aatgatgaac atgtccgcag
    15541 tctcaatgtg tctaataccg tctgcatgat tgtctatgag gctcttcgcc agcagaactt
    15601 tgcaggtctt gagctcgtcc atacctatga agtggataaa ttgaaataaa aaatgaaaat
    15661 gaacaaaatg cttgcgcttg caagcgtttt ttgttatgat aaaagagtct tcagggcagg
    15721 gtgtgattcc cgaccggcgg tgactttaac taggaaatga tcttttcctt ttatactttg
    15781 ttgacaagct ttgcctaacc agaagttatg cctacagctt gtcgcctagt ctaaaagaaa
    15841 aatctctatt tccttctctt taaagaagtc cgcgagcgca agctgatgtg gtgagattcc
    15901 acaaccgaca gtatagtctg gatgggagaa gacgaaagaa tagctttgtc tgttttgata
    15961 gatttatagc cggattgtaa ccacttgctt tgtttttctt taatagagta agagggaact
    16021 tttgggatat aaaaagtgag aatagataga ggaatccttt ccaacttctt ctgattttat
    16081 agaaaattgg aggaacctgt tatgacaaac acacgtcgac tttcgaccat tgcaatttta
    16141 tcagccatct catttgtgct gatgtacttt gactttccgc ttttaccagc ggcatctttc
    16201 ctcaagatcg aatttagtat cttgccagtc cttgtgggtc tggtggtcat ggatttgcct
    16261 gctgctctag gagtgctctt gcttcgctca ctattgaaat tgcttcttaa cagccaagga
    16321 gtgaatactt acattggttt gccgatgaat atcgtagctt tgggagtttt tgtcatcgta
    16381 tttgctttga tttggaaaaa ggaacggaca ccccttcgtt tcctactagg ctctctagct
    16441 ggaactgttg gtttaacctt ggccatgttg gttctcaact atgtttatgc tgttcctttg
    16501 tacgctaagt ttgctaactt tgatattgga aaaattttgg gactttccaa ctacctaatg
    16561 accatggtat taccttttaa cttgattgag ggtgtaatct tttccgtttc attctggttg
    16621 ttgtacgtcc ttttgaaacc aaccttaaaa cactatgaaa gataaacaaa catttttaat
    16681 gaagggcagt tttgcccttt tacttttcgt tattcttggc tacatggtca aattttaccc
    16741 tgaaacgctg gtcaattttg accaatcgat tcagactgcc attcgaggag acttaccaga
    16801 ttatttgact attctttttc gagccctcac acgtctgatt gatatcccag tgattatcac
    16861 ttgggttgtc attacagctt ttgtctttta tcgtaagcga tggaagatag aaagtttctt
    16921 catgctggga aatctggctt tggcaggtct tttaatcgtg acctttaaaa atatctacca
    16981 gcgcccacgg ccagatatct tacatctggt ggaggagaag ggattttcct tcccaagtgg
    17041 gcattctctg gctgtaacct tgatggtagg tactttgatt gttattctta gtcagcggat
    17101 taaagatcca gtctggagaa aaatcgtaca aatcgtcctt ggcctctacc tagtcagtgt
    17161 gctggtatca agagtctatc tgggagttca ttacccatca gacgtccttg ccagtctctg
    17221 tgtgggcttg ggagtcctgt ttatcgagtt tcccttctat gacaagctcc gcttccaatg
    17281 gcgatttaaa ggcaaacaga agtgagtatc gaattccctt gaggagaaag aaatgaaagt
    17341 caacatagca gatcttcatc cgactcaact atatttatca gaaaagaagt tgcaagatat
    17401 tcagatgctt taccagtcgg cagaaacaat ccaagtcgat ccaatcagta ttcttgcctt
    17461 tggagattgt ttgctgatta cagatgggca tcacagggct tatcagactt tattggcaga
    17521 tcgggatacg atttctgctg agtgggatag agatggtggt gatgaactat atcatctcta
    17581 tgcgcaagct tgcgaggaaa gaaagattta ctctgttttt gatttaaaaa atcatatctt
    17641 acctctttgg atcggcatgg tgagcttttt tggattgaag tcaaggagaa gggagaatgg
    17701 tttccaattg gggatattac actatctcag gataatctcc ccattgtgat tgggaattcc
    17761 gcttaccaac atcgaggact tggaaaaaag attctaagtg ctttgattga attggctcga
    17821 gtaaaaggat ggaaagaatt gagagtcaag gaaatctaca cctacaatca tgcttctagg
    17881 aggtgtttca agtcgcttgg atttgtggaa aatggagcaa cagaaaaagg aaggagtttt
    17941 atattggaat tagtctaatc ctgccacttt ctccccctta acctttgact attccgcaaa
    18001 attatcgtaa aataaagagt aaatgataaa atgaggtcag agtctgttcg ctctgacgat
    18061 agtagtataa atgaggagaa acgctttgaa attagaagta tttgctgggc aagaaaaaag
    18121 tgaactatct atgattgagg tagcgcgtgc tatcttggaa cttcgtggtc gcgatcatga
    18181 gatgcatttt agcgatcttg taaacgaaat tcaaaactac cttggaacat caaacagcga
    18241 tatccgcgaa gctttgcctt tgttctacac agagttgaac tttgacggta gcttcatctc
    18301 acttggggac aacaaatggg gtcttcgttc atggtatggt gtggacgaaa tcgacgaaga
    18361 aatcatcgct cttgaagaaa atgacgacga tgaagtaaca ccaaaagcta agaaaaaacg
    18421 tgtcaatgcc tttatggatg gtgattcaga tgccattgac tacaatgcag atgatccaga
    18481 agacgaagat gcatacgaag cagatccagc tctttcatac gatgatgaaa atccagatga
    18541 tgaaaaaaat gaagtggaag cttatgatgc agaaatcaac gaaatcgctc cagatgactt
    18601 gggagaagat gtggatctca acgaagacga cgacgagttt tcagatgacg acgctgaaac
    18661 cagcgaggaa taagagttag ctattgacaa ttatcctatt tttaggtatc atattgtttg
    18721 ggcacctctt ttagaggtcg gggctcccta gttcttaggg agctattttt gttttttcaa
    18781 gaagttatct tcttgtattt tatactcaat gaaaatcaaa gagcaagcta ggaaactagc
    18841 cgtaggctgc tcaaaacact gttttgaggt tgtagataag actgacaaag tcaggaacac
    18901 atatctacgg caaggcgacg ttgacacggt ttgaagagat tttcgaagag tattagttgt
    18961 gaatctggtg cagtcgtccc agattattct tattagtagg gtcttgtttt ctatatcccc
    19021 tcgtagttaa caagaccttg agcattttag aaagaggaat ctatgtctac gaaatatatt
    19081 tttgtaactg gtggtgtggt atcgtctatt gggaaaggga ttgtggcagc gagtctaggc
    19141 cgtctcttga aaaatcgtgg tctcaaagta accattcaaa agtttgaccc ttatatcaat
    19201 attgatccgg gaaccatgag tccttaccag cacggggaag tttttgtgac agatgacgga
    19261 gctgagacag atttggactt gggtcactat gaacgtttca tcgatatcaa tctcaacaaa
    19321 tattccaacg tgacaactgg gaaaatttac agtgaagttc ttcgtaaaga acgccgtgga
    19381 gaataccttg gggcaactgt tcaagtcatt cctcatatca cagatgcttt gaaagaaaaa
    19441 atcaagcgtg ccgctctaac gaccgactct gatgtcatta tcacagaggt tggtggaaca
    19501 gtaggagata tcgagtcctt gccattccta gaggctcttc gtcagatgaa ggcagatgtg
    19561 ggtgcggata atgtcatgta tatccataca accttgcttc cttacctcaa ggctgctggt
    19621 gaaatgaaaa ccaaaccaac ccaacactct gtcaaagaat tgcgtggctt gggaatccaa
    19681 ccaaatatgt tggttattcg tacagaagag ccagctggtc aaggaattaa aaataaactg
    19741 gcccagttct gtgatgtggc accagaagcc gttatcgaat cgttggatgt tgaacacctt
    19801 taccaaattc cactgaactt gcaggcacaa gggatggacc aaattgtttg tgatcatttg
    19861 aaattagacg caccagcagc ggctatgaca gaatggtcag ccatggtgga caaggtcatg
    19921 aacctcaaga aacaagttaa gatttccctt gttggtaagt atgtggagtt gcaagatgcc
    19981 tatatctcag tggtcgaagc cttgaaacac tctggctatg tcaatgatgc agaagttaaa
    20041 atcaattggg tcaatgccaa tgatgtgaca gcagagaatg tagcagaact cttgtctgat
    20101 gcagacggga tcatcgtacc aggtggtttt ggtcaacgtg gtacagaagg gaaaatccaa
    20161 gccattcgct atgcgcgtga aaatgatgtt ccaatgttgg gagtctgctt gggaatgcag
    20221 ttgacatgta tcgagtttgc tcgtcacgtt ttaggtcttg aaggtgccaa ttctgcagag
    20281 cttgcaccag aaacaaaata ccctatcatt gatatcatgc gtgatcagat tgatattgag
    20341 gatatgggtg gaacccttcg tttgggactt tatccgtcta agttgaaacg tggctctaag
    20401 gctgctgctg cttatcacaa tcaagaagtg gtgcaacgcc gtcaccgtca ccgttatgag
    20461 tttaataatg ccttccgtga gcagtttgag gcagcaggtt ttgtcttttc aggagtttct
    20521 ccagacaatc gtttggtaga aatcgtggaa attcctgaaa ataaattctt tgtagcttgt
    20581 cagtatcacc ctgaactgtc aagccgtcca aaccgaccag aagaactcta cactgccttt
    20641 gttactgcag cggttgagaa cagcaattag caaaatcaga acctttgaga aaaatctcag
    20701 aggttttttg catacgatga tattgcagta tatctgaggt aggggtcctc tgtatgtacc
    20761 tgctaccgtt gaaatcaata gcgactccct cttgccctgt gctagtgaat ggatttatca
    20821 gtatattgaa atgaaataaa atttgaacaa attaattcgg aaagccaaat caatttctag
    20881 caaagtttta ggaactggat t
//
LOCUS       DAVKJD010000030        20705 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKJD010000030 DAVKJD010000000
VERSION     DAVKJD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20705
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.030"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(94..243)
                     /locus_tag="VW157_001113"
                     /pseudo
     CDS             complement(94..243)
                     /locus_tag="VW157_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(397..2028)
                     /gene="rlmD"
                     /locus_tag="VW157_001114"
     CDS             complement(397..2028)
                     /gene="rlmD"
                     /locus_tag="VW157_001114"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9552426.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHDLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVIIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2102..3076)
                     /locus_tag="VW157_001115"
     CDS             complement(2102..3076)
                     /locus_tag="VW157_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9552427.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3150..3764)
                     /gene="pmp23"
                     /locus_tag="VW157_001116"
     CDS             complement(3150..3764)
                     /gene="pmp23"
                     /locus_tag="VW157_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9552428.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3764..4738)
                     /locus_tag="VW157_001117"
     CDS             complement(3764..4738)
                     /locus_tag="VW157_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9552429.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4746..6002)
                     /locus_tag="VW157_001118"
     CDS             complement(4746..6002)
                     /locus_tag="VW157_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9552430.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFLLYED"
     gene            complement(6063..6537)
                     /locus_tag="VW157_001119"
                     /pseudo
     CDS             complement(6063..6537)
                     /locus_tag="VW157_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(6539..7141)
                     /locus_tag="VW157_001120"
     CDS             complement(6539..7141)
                     /locus_tag="VW157_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9552431.1"
                     /translation="MMDRIRQELENGGAVILPTETVYGLFSKALDEKAVDHVYRLKRR
                     PRDKALNLNIASFEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7125..7964)
                     /gene="prmC"
                     /locus_tag="VW157_001121"
     CDS             complement(7125..7964)
                     /gene="prmC"
                     /locus_tag="VW157_001121"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9552432.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPAWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDATDYLKDSGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            complement(7964..9043)
                     /gene="prfA"
                     /locus_tag="VW157_001122"
     CDS             complement(7964..9043)
                     /gene="prfA"
                     /locus_tag="VW157_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9552433.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9053..9532)
                     /locus_tag="VW157_001123"
     CDS             complement(9053..9532)
                     /locus_tag="VW157_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9552434.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDVIIGFVMIAYDKKIGISSGNYLLFRFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            complement(9522..10121)
                     /locus_tag="VW157_001124"
     CDS             complement(9522..10121)
                     /locus_tag="VW157_001124"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9552435.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            10244..10426
                     /locus_tag="VW157_001125"
     CDS             10244..10426
                     /locus_tag="VW157_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9552436.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10563..11936
                     /gene="mnmE"
                     /locus_tag="VW157_001126"
     CDS             10563..11936
                     /gene="mnmE"
                     /locus_tag="VW157_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9552437.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDATPDELITQLF
                     SQFCLGK"
     gene            complement(11983..12918)
                     /gene="dapA"
                     /locus_tag="VW157_001127"
     CDS             complement(11983..12918)
                     /gene="dapA"
                     /locus_tag="VW157_001127"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9552438.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLDHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKIVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKSEEFLVYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFIAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12974..14050)
                     /locus_tag="VW157_001128"
     CDS             complement(12974..14050)
                     /locus_tag="VW157_001128"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9552439.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGVIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14392..14464)
                     /locus_tag="VW157_001129"
     tRNA            complement(14392..14464)
                     /locus_tag="VW157_001129"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14429..14431),aa:Thr,seq:ggt)
     gene            complement(14564..15073)
                     /locus_tag="VW157_001130"
     CDS             complement(14564..15073)
                     /locus_tag="VW157_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9552440.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15071..15167)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15196..15627
                     /locus_tag="VW157_001131"
     CDS             15196..15627
                     /locus_tag="VW157_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9552441.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15709..16086
                     /gene="mscL"
                     /locus_tag="VW157_001132"
     CDS             15709..16086
                     /gene="mscL"
                     /locus_tag="VW157_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9552442.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16128..17222)
                     /gene="hemH"
                     /locus_tag="VW157_001133"
     CDS             complement(16128..17222)
                     /gene="hemH"
                     /locus_tag="VW157_001133"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9552443.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQILYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSKQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTIRINENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17376..18596
                     /gene="pepT"
                     /locus_tag="VW157_001134"
     CDS             17376..18596
                     /gene="pepT"
                     /locus_tag="VW157_001134"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9552444.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSNRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVRSL"
     gene            complement(18639..19268)
                     /locus_tag="VW157_001135"
     CDS             complement(18639..19268)
                     /locus_tag="VW157_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552445.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENKTTESSTTTGNQEKPVE"
     gene            19233..19454
                     /locus_tag="VW157_001136"
     CDS             19233..19454
                     /locus_tag="VW157_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552446.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(19531..20073)
                     /locus_tag="VW157_001137"
                     /pseudo
     CDS             complement(19531..20073)
                     /locus_tag="VW157_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521835.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(20177..20705)
                     /locus_tag="VW157_001138"
                     /pseudo
     CDS             complement(20177..20705)
                     /locus_tag="VW157_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         5990 a         4334 c         3889 g         6492 t
ORIGIN      
        1 atttattttt tgagttttaa actttggtag aagtcttgaa ctccttatat ttacataact
       61 atttgcctct ataactgtta tgacacgaaa ccaacaagca agttcaagca cctccatatc
      121 tttattcaaa tgaggactaa gatattcaca aactttatca taaacctctt tatctttttt
      181 cataaaaggg gcatacaact tagcaaattt atcctaaaat tttttgttgc ctttatcaat
      241 catttcaacg ctcctttaaa tataaaaatg atcctatcaa tctttatatt gagcctattt
      301 tctaattgat accttgcaag aaaggaataa tagctgttcc tataccaagt aggaatgtaa
      361 taaatgcaga aagtcatata ctgtcattct tttctattaa atcattttga agtgtctcaa
      421 agcatccatg atggcttctt ctttttcagg tgtacactgt ggaataattt gtttatcctt
      481 tttagacttg ttgtaatgtt ctcgtaattc tattccacat ttctttttta tctgtgcaat
      541 atataatgtc gaaactttta attcaaattt attccaaaca tattctttga tttgagcata
      601 tgttgctttg ctctccgcac ttgtcaaatc catctcatcc agctcaattt caacacttat
      661 gtgcttatcg acatcgagtt tggacaaaag tgctaccgtc tcgacgtgat gcgtttgagg
      721 aaatagatcc accggctgga ctttcttcaa ttcatatccc aactcttggt atagtttaat
      781 atcacgcgcc atggttgcga cattgcagga gatataggcg atgcgatcgg ctcctgtttg
      841 ggcgcttgct ttgataaagc tttctgtcaa gcccttgcgt ggaggatcaa ccaagataac
      901 ggttggttga ataccttcct tgagccattt cttcatggca ttttcagccg tgtcacagac
      961 atagtgggca ttagtaatct tgttcaaaga agcattcttc tggctattct ctactgcttc
     1021 tggaatcagt tcaacaccgt agacttcttt gacatgcttg gcgactgata aaccaatggt
     1081 tccaatacca gaataggcat caataatcac atcatctttt tttaactctg caaagtcaat
     1141 ggctgtttga tagagtttct ccgccatttc agtattgact tggtaaaagg ctgggccagc
     1201 gatttggaag tcatttccca acatctggtc cgtaatatag tcttgaccat aaagagtgcg
     1261 ccactcctta ccaaaaatcg cattggtatt ctggtcgttg atattttgca tgacagacac
     1321 aatctctggg aactgcttga taacttgttc aatcaattgg tcaacacgaa aaacttttgg
     1381 acgagttgtc accaaaacga ccatgatttg tcctgaatag tgaccacgac gcaccacaag
     1441 attccgaatc aatccagact gttccttttc gtcataaggt tttaaatcaa aacgacggag
     1501 caggtctcga agagctacta cgacttggtc aatgacagga tcctggataa agaaatcttc
     1561 aaggggcatg aggtcgtgcg aattcttacg gaaaaatcct gtttccaaga caccattcac
     1621 tcgacgaacg ggcacctgcg ccttattgcg atacttgact ggatgttcca taccaagcgt
     1681 ttcagcaact tctacatctg caattccagc aatcttgtag agactgtcct tgacttgctt
     1741 ggttttaaac ttgagctgtt ctggataaga aaggtgtcct aaatccgcga ttcctgaacg
     1801 caggtaagcc aaatctagat cttgattacg gtgtggtgac tggacaaggt atttttcaac
     1861 ttttccaaag ccaatctttt tattgacctt gaggacacgc atgagaattt tttcactcgg
     1921 taaagcattc tctacaaaaa agaccaaacc atctaccttg gcaactcctg ccccttcatg
     1981 ggtcaagtca acaatttcaa cttctacaat atcatttttc tttaacattc ttttctcttt
     2041 ctattggccg acaaaaaaga cggcaacgtt acggttacca tccattatac catgaaattt
     2101 cttaagaacc aaaactcttg aagaagttga caagggcttg ccagagggag cttaagaagt
     2161 taccaccgtc ctctagcgcc ttatccgcat caaagttaag gttgatattt ttaaaactgt
     2221 cgccagcttg tgatacgatg ctttgtttaa ggtcatttag ggttttagtg aaatctgcat
     2281 tgctgaggat atcactcttt gagagattca aggcaaaatt gatgatgata ttgatctggt
     2341 ttcctgttat gacctgatca agtttgtaat tttttaaggt atcttcaaca atcttgcgga
     2401 tatcttcttc tgtcagattt cccttgcttt ctttagcttt ggcgagtcct gacttgatat
     2461 cagctagggc aacgtttaat ttattagcat catagcctga tttgtccttg ttttcagcat
     2521 tgatatctga caaagctttt agctcttctt gagccaaatc tttattagct tgtggcacct
     2581 tggctccatt agcctctagc gaatagtaaa tccctgctaa agcactttct cctgtaactg
     2641 gaataggggc tgctacagtg attttggcat gttccatacc cagcgttact gctgcgtttc
     2701 ggtacatatc ctgagtcacc ttagtgatat tttctggtgt ttcaatcttg acctcaagtg
     2761 gcgatttgtc acctagcttt tgaatcttgg ctgatgaata caactgtaag ctagagtcat
     2821 tggccacatt catgatttta gaataaacat caggtgtcat ggtcttgagt tctttggtat
     2881 ctgttgaggc attgtagccc agttttttaa gagtttgatt tttttggtct tcagataggg
     2941 aggaacctag gacatattca ggttggacat aggtttcatc gataactttt tgaacatctg
     3001 ttgctgcatg gacgctattc atagctgtta ctgcccacaa gaccgcagca ctagtcagaa
     3061 agagtttctt tctcataggg aatttcctcc tttacttctt tatagtaata tatctatctt
     3121 aaagaaaact tataacaaaa acacctggtc tagccagatg ttgaaaagag agtgaaacat
     3181 ttgatgatgt aaaggttaag tcgtacctgt ctagaataat aataatttcc tccatttaca
     3241 tagagttcag caccgtgaaa aatggaaatg gggtgaatat aactataagt ctttccagtc
     3301 gtattaccaa gcaagggggc aacagtctca cgagagtact gtttggctag agccaaggta
     3361 ttttccttgc cattttgggc gataaaatcg atataggcag gtccaaaatt ataggcttga
     3421 acagctgtcc agatatctac ccccttcttc tgcgccagat agagattgcc tgtcagagtt
     3481 tgaatgcctt gccgaatgct agaggcatta tcattgatgg tgttggtgga accacttgca
     3541 gactcactag actgcataac atcgccttct tttccttttg tttcagtata aatcatagca
     3601 agcacaagct cttcgtttgc tggggtgtct ttttcactca agatttctcg caccatgggt
     3661 tgataggtca tgacttgttt gacatcttga tgaacgcggt aagctttata gccagcaaaa
     3721 aggaagactg ctagtacaag cactcttcga attcgtttaa acattattta ctttggatat
     3781 cctcgatatt tttgattaag atagagtagg ttccattttc gttttggata aactcaacag
     3841 actcggcgtc ttgatagacg ttattgggaa cgatgagctc aattccattt gataaggaga
     3901 gtttttggtt ttcaaatttc tttaattggc gacttgcatc aatttcatca aattgaacag
     3961 gttctggtac ggcttctttg acttggtcaa taaagctcaa acgagccgtc agattgttgt
     4021 caaaaaggtc attagccaat ttctcaggtg acaattcatt gctttcttct aggttgttga
     4081 aaatcgctga tttgaccttg gattgaaatt gaaaatcatc tgtgttaaaa gattcagcaa
     4141 ttctctgggc tgttttttcc agttccttga tagatttttt aggagaaatc ttaggagcga
     4201 cagcaagaag attatctgaa aaatagttca aaaaagtccc gttgtacttg attcgttttt
     4261 caatcaggtg atacttgcga ctctgaagat tgaccaccaa ggcctcgtca gcacccgttc
     4321 caaatccagg caggttattc tgagtcagct tgattggatt atcaacttct cctccgaggt
     4381 gggtcaaggt ctcccgcagg gcaattcgca agaaagcgaa atgttctaca ccttctttag
     4441 aaaattgaac aaaaatcaag tcattggtct tgagattttc agaaatgcta aactcctctt
     4501 tccagagatt agccagcgtt actgatgtct ccaacaaatc gtctgtaata tgattgaaga
     4561 agggattttc ttcttcgaaa atcccagtct tggcttcatc tgaatacaca tgttcaattt
     4621 ttttacgtag gtattcttcg atttttggag taatattgag aaacttatct gctaagaaca
     4681 gttcggtatc atccggactg aactggtgaa taatggcttt cttaatataa atgtccataa
     4741 aagttttagt cctcgtatag taggaaggca tctgtcaatt ctttgactgc acttctcact
     4801 tcttctaata cagcctcatt ttctgaattc ttaagggttt taatgatgag ttcagccact
     4861 ttgcgacttt cttcttcacc aaatccacgt gcagtaatgg ctgctgctcc gatacgaatc
     4921 ccacttgtct tgaatggtga caagctttcg taagggattg agtttttatt taaggtaata
     4981 ttgacttcat ccaacaagtt ttgagcaact ttgccgtttt ctacaacttt agtcacatca
     5041 acaaggaaga gatggttttc agttccacct gaaataatac ggaaatcagg gtcttgcaag
     5101 aagacatctg ccatagcctt gctgttctta attacattgg cagcatattc cttgaaggct
     5161 ggatccaaaa cttctttgaa ggaaactgcc ttagccgcca caacatgctc taaaggaccg
     5221 ccctgaatac ctgggaaaat agctgaattg atttttttag caagttcttc gtcattggtc
     5281 aaaatcaaac caccacgagg accacgaagg gttttgtggg tcgttgttgt tgtgatatga
     5341 gcgtatggaa ctgggcttgg atgaaggcca gccgcaacca aaccagcgat atgggccatg
     5401 tccaccatga gcttcgcccc aacagcatct gcaatttcac ggaattttga aaaatcgata
     5461 atttgagaat aggctgaagc accagctaca atcagttttg gttttacttc ttgggcttgt
     5521 ttcaagatag catcaaagtc taagagttcc gtttcaggat ccacactata agaaacaaag
     5581 ttgtaggttt gaccagagaa gctaacagga gccccatgag tcaaatgacc acctgatgcc
     5641 aaatccattc ccataaccgt atcacctggc tcaatcaagg acatgtaagc cgcacagtta
     5701 gcttggcttc ctgaatgtgg ttggacattg gcaaatttag caccgaaaat ttcttttgcg
     5761 cgttcaatag caagagtctc tacaacgtct actacatcag ttccaccata ataacggcgt
     5821 cctgggtaac cctcggcata tttgtttgtc aagatagacc cttgagctgc cataacagcc
     5881 ttggaaacta cgttttccga agcaatcaac tcgatattat tttgttggcg ttcttcttct
     5941 ttagcaatag cattccagag atcagcatca tatgctttaa aatcatcttt gtcaaaaatc
     6001 ataggtcttc tcctttattg tgtgactagt ccattagttt gattttacaa taagaaaata
     6061 aactaaaaga tgcgaataaa ccgtttctgc actttatcac aagtataacc aactttttca
     6121 taaaatgcat gagcacccag acgatgattg gcagaattta agcggataaa cccataacca
     6181 catcttttgg cttcttgttc caacccttgt agtaaacttt taccgatacc ttgaccttgc
     6241 gcttgaggtg aaactgctaa agctaagata ttaaatcctg ctttggagag gtgaaactgc
     6301 taaagctaag atattaaatc ctgctttgga atagagtgat tcgtaaactt cagcgtggac
     6361 atatccaagt aagacatgat tagctgcatc ctcatagcca agtaggaaat gatgggaatc
     6421 ctgagacagt ctagctagtt ggctagccgt ttcctctgga ctaaaagtat aacccaaagc
     6481 ctcttggttg atgtcacata tcgcttttac atctgtttct tgcaaatctc ttagcatttc
     6541 aagcctcctc aaaagaaatc tctggcaacc gagcaagaat atcttctcgt ttaattgcgc
     6601 cttggcgtaa gattttcacc ttgtctccag acaaatccac aatagttgaa tcctgtccag
     6661 ttagaaaagc atcgtcttcc agacccagaa cctcttggtc aaaatccttc agaatttgtt
     6721 caaaggttac accacttgcc tgacctgaga tattggcaga cggcccaatc aagggacctg
     6781 tctctcgaat taaatccagt gtgatagggt gactgggcat ccgaaatcca atagttgcaa
     6841 ggtcagaatt tacccaatag ggaactcggt cattggcttc gagaataatg gtcaagggac
     6901 ctggcaaaaa ggtctctaca agtttttgta gataagctgg ctgattcttt gaaaagtgca
     6961 agatgtcctc gaaagaggcg atattgagat tgagtgcctt atctctagga cgacgtttga
     7021 gtcggtaaac atggtcaact gctttttcat ctaaggcctt ggaaaaaaga ccataaaccg
     7081 tctctgtagg cagaatgaca gctccaccat tttccaactc ttgtctaatc ctgtccatca
     7141 tcaaccacga ccatcctatc ttgaccaaat tggtccttga gtgttcgaac tcttttttca
     7201 ggaagatgtt tcctaaaaag ttcgggaaca ctttgacctt gcttgtatcc aatttcaagg
     7261 taaatcttac cactatcttt gagatagtct gttgcatctt ccgcaattct acagtaaata
     7321 gctaggccat cctcgtctgc aaagagagct agatgaggct ccgaatgcaa aacattcaag
     7381 cctacctctg actcatcttc acgagagata tagggtggat tggatacaat tatatcatat
     7441 ttttcagaaa tttctgcaaa acaatcagat tttttaaaaa atatattaag attttgatcg
     7501 ctagcattct ctgatgcaag ctctaaggcc tcttgagaaa catctgctgc tgtcactgac
     7561 caagctggtc tattttttgc tagagcgaga gcaatagctc cactacctgt tccaatatcc
     7621 agaactgaga gattcgtctc aagattttca gttagaataa gctccactaa ctcctctgtt
     7681 tctggacgag gaatcaaaac ccgttcatcc acctttaaat gcattccata aaaatctgcc
     7741 tgtccaatga tgtactgagc tggcttgtga gctgctagtt gctggtaaat atcttctaca
     7801 aattgttttt cttcctctgt tgtcacctcc tgctggaggg caaaaataaa gtctgtaaaa
     7861 gatagatttt tcagactacg atagacaaaa gagaggcttt ccgcttcctc tccttgtctt
     7921 atcaactctt cttcaaaatt tgaaaataat tgagctaatt tcattatttg tttaattctt
     7981 ctagtttttg tgtttggtca taaagcacca aggcatccac aacttcgtcc aatttaccag
     8041 acaaaatcgt atctagtttt tggagggtca agccgatacg gtggtctgtg acacggtttt
     8101 gtgggaagtt ataagttcgg atccgttctg aacggtcacc agtaccgatt gtcgacttac
     8161 gctcagcgtc ttgttcatcc tgagcaatct gagcaaagtg gtcagcgacg cgtgcacgga
     8221 taatcttcat agccttctcg cggttcttct gctgggtacg ttcttcctgc atctcaacct
     8281 tgatattggt tggcaagtga acgatacgaa cggcagtcgc aaccttattg acgttctgtc
     8341 caccagcacc agaggcgtga tagatgtcga cacgaaggtc ttttggatca atgtcgtatt
     8401 caacctcttc aacttctggc ataacaagaa ctgtcgctgt cgaagtatga acacggcctt
     8461 ggctttctgt cacaggaaca cgttgcacac ggtgggcacc tgattcatac ttaagcttag
     8521 agtatacaga ctgacctgaa accatagcaa ccacttcttt aaaaccaccg acaccattca
     8581 tagaggcttc catgacttca aagcgccaac cttgggcttc cgcatacttt tggtacatag
     8641 ttagcaaatc tccagcgaaa agtgccgctt cgtctccacc agctgctcca cggatttcaa
     8701 ggatgatatt cttgtcatcg tttggatcct ttggaaggag caaaattttc agtttttctt
     8761 catattcttc tttttcagcc ttggcatctt tgagttcttg cttggccatt tcttccaagt
     8821 ccgcatctcc gcctgattcc ttaatcatct cttcggcatc gacgatattt tgaaggactt
     8881 gtttatactc acggtaggct attacggtgt cacgattgga agcttcttct tttgaaagct
     8941 ccataaaacg cttggtgtct gaaacgacat cagggtcact cagcaattct cctagttctt
     9001 cataacggtc ttctacagct tgtagttgat catagatgtt cattttttct ccttatttct
     9061 caattgttaa atcatagatt gctactactt cattctcgga tatttcccca gtttctttaa
     9121 atccataact gagataacaa aatcttgcct gttcattttc tggttcataa gacaaccaaa
     9181 gtttattgct taaacctgct ggcgctgttc gaacatagtc tagtacttta tccataattg
     9241 gtttaaaata tccttgattt tgaaaattct tatcaatcat aaaacgaaat agtaaataat
     9301 ttccactact aattccgatc tttttatcat aagctatcat cacaaaacct ataattacat
     9361 cattatcata aactgccaat ggagctacaa aatctccatt tttagtgtag acgtatgctt
     9421 cagctaaact aattgcgttg gttgcaatga attgtttttg atattccttg acatccaaat
     9481 ttaaaacatc aaaataattt tccattgtaa catctcttag ttcaattgtc atagttttgc
     9541 tccttgttag aggttatcat tggcgcaaaa taatgtttac ggcaaaccga gatataggtt
     9601 tcattaccac caatctggat ctgttctcca tcgtaaacgg gcagtccatc ctgtgttcgc
     9661 aacaccatgg tcgccttttt cttgcaatac tgacagatgg tcttgatttc gtcaatcttg
     9721 tccgctaaaa gcaagagata tttggaacct tcgaacaatt cattgcgaaa gtcatttttc
     9781 aagccaaaag ccatgacggg tatgtctaac tcgtccacaa cacgagctag gtcgtaaaca
     9841 tggtggcgtt taagaaactg ggcttcatcg accaaaacac agtaaggttt ttctggtagg
     9901 tctcggatat agccaaagat atccgttgtt tcctcaatcg caagggcagg gcgtttcatg
     9961 ccaattcgac tcgacacata gccaacaccg tcacgcgtat ccagagccga ggtcataatg
    10021 acaacacctt ttccttgctc ctcgtagtta taggccactt tgagaatctc aatcgtttta
    10081 ccagagttca tggtcccata acgatagtac aactgtgcca tgtttcttgc ttcacgtcca
    10141 tttctaaatt tttgctacat tctagtatat cataattttc ttaagcttta aacggcaaaa
    10201 tgtggtaaaa tggaagaaat caaaactagt ggaggaagct attatgccat ttgtacgcat
    10261 cgatttattt gaaggacgca cgctcgagca aaagaaagct cttgctaagg aagtaacgga
    10321 agcagttgtc cgcaacactg gagcccctca atctgctgtc catgtcatca tcaacgacat
    10381 gccagaagga acttacttcc cacaagggga aatgcgtact aaataagcta gcttaagcag
    10441 aattgcttag gctttttcaa tctccaagta gcattcattg aagaaatatc ctaaatttgt
    10501 tacaatttga aaagaaactt ggagaatttc caagaaaaga gctattaatt aaaggaaaca
    10561 ttatgattac acgtgaattt gataccatcg ctgctatctc tactccacta ggtgaagggg
    10621 ctattggtat tgtccgcctg agcggaacag acagttttgc tattgcgcaa aagattttta
    10681 aaggaaaaga cttgaacaag gttgccagcc acactctcaa ctacggtcac attattgatc
    10741 ctctgactgg taaagtcatg gacgaggtta tggttggggc tatgaagtct ccaaagacct
    10801 tcactcgtga ggatattatc gagattaaca cccacggtgg gattgcggtg accaatgaaa
    10861 ttctccagct agctattcgt gaaggggctc ggttggcaga acctggtgaa tttaccaaac
    10921 gtgctttttt aaacggtcgc gtagacttga cacaggcaga ggctgtgatg gatatcatcc
    10981 gtgccaagac tgacaaggcc atgaacattg cggtcaaaca attagacggc tccctttctg
    11041 acctcattaa caatacccgt caagaaatcc tcaatacact tgcccaagtt gaggtcaata
    11101 tcgactatcc tgagtatgac gatgttgagg aagccactac tgctgttgtc cgagagaaga
    11161 caatggagtt tgagcaatta ctaaccaaac tccttaggac agcacgtcgt ggtaaaatcc
    11221 ttcgtgaagg aatttcaacg gctatcattg gacgtcccaa cgttgggaaa tcaagccttc
    11281 tcaacaacct cttgcgtgag gacaaggcta tcgtaacaga tatcgctggg acaacacgag
    11341 atgtcatcga agagtacgtc aacatcaatg gtgtacctct caaattgatt gatacagccg
    11401 gtattcgtga aacagatgac atagtggaac aaatcggtgt tgagcgttcg aaaaaagccc
    11461 tcaaggaagc cgacttggtt ctactagtgc taaatgccag tgaaccactg actgcgcaag
    11521 acagacaact tcttgaaatt agccaagata ccaatcgcat tattctactt aataaaaccg
    11581 acctgccaga aacgattgaa acttcgaaac tacctgaaga cgttatccgt atttcagtcc
    11641 ttaaaaacca aaacatcgac aagattgaag agcgaatcaa caacctcttc tttgaaaatg
    11701 ctggcttggt cgagcaagat gctacttact tgtcaaacgc ccgtcacatt tccctgattg
    11761 aaaaagcagt tgaaagccta caagccgtta atcaaggtct tgagctgggg atgccagttg
    11821 atttgcttca agttgacttg actcgtactt gggaaatcct cggagaaatc actggggatg
    11881 ctactccaga tgaactcatc acccaactct ttagccaatt ctgtttagga aaataagaaa
    11941 aatccatgat ccttcattcg gtcatggatt ttattgtctt tattagtaat ctggtcttaa
    12001 gacacctgtt acagttgcct tggtcgcttc gtagtcgcca tctacgacaa ccttgataat
    12061 gcgtttagca tcttcttctg gtgctggaac aagaggtaga cgagttggtc cagcttcaaa
    12121 tcccatgtag ttcaaaactg ccttaactgg agcaggactt ggataagaga agagagcatt
    12181 aaccttagga atgaatttac gttgaatagc tgcggctttc ttcatatcac tttctgcaat
    12241 ggcaataaac atctcgtgca tttcatcccc atttgtatga gaggcaacag aaataacccc
    12301 atccgcccca aggttcatgg catggaaagc atctccatcc tcaccagtat agaccaagaa
    12361 ctcttctgat ttgtgctcaa tcaagtaagc catattagcc aagctagtac attctttgac
    12421 accgataata tttggatgat cagccaagcg aagcatggtt tctggagtta attcaacaac
    12481 cacacgccct ggaatgttat agataataat tggtaggtca gaagcatctg caatggcttt
    12541 aaaatgctga tacattcctt cttgagaagg tttgttgtag taaggaacaa tagcaagacc
    12601 agcagcaaaa ccgccgaatt ccgctacttc tttgacaaac tcgatagagt cacgcgtatc
    12661 attagtacct acacccgcaa tcaaaggaac tcgtccattg acaatctttt gtacagccgc
    12721 aaataactcc aactcctcat cgtgggtcaa agttggactc tcagcagtgg tccctgcgag
    12781 aagaattcca tccgtatgat ggtccaataa atgctcaatc aaggctggaa tagcatcaaa
    12841 gttgatggaa ccatcctcgt ggaagggagt aataaaggct gtgatgattt tacactcttt
    12901 taaatcttga taagacatga aaacacctct ctatttcaaa gaaggagttc atctgaactc
    12961 ctaaacgata tgactatttt aattcaaatt tcaattcagc tgttggacga accaatccac
    13021 gttcatgaag agtttcagca atctgaactg agttccaagc agcacctttg agaaggttat
    13081 ctgaaacaac ccacatgtga attccttttt ctgcatccaa gtctttacgg atacgaccaa
    13141 caaaggtatc acgtgaacca actgcattga tagcttgagg atagatttga tgagctacat
    13201 catcttcaag aacagcacct gggaaggctg cgatagctgc ttttacttct tcgattggag
    13261 ccacttcttt tgtttcgata taaacagact cagagtgagc tgacaagact ggaatacgca
    13321 cacatgttgc agatactgca atgctatcat cttccataat tttcttagtt tccttggtca
    13381 tcttcatctc ttcgtacgtg taatcattat cagtgaaaac atcaatttgt ggaagagcat
    13441 tgaaggcgat aggataatgt ttcttgtcac cacctgaagg caagatttcc gcatgcaaat
    13501 cacgtggttt cacaccatca ttcaagactt cacgaagttc acgttgtgtc tcaagaattg
    13561 ctcccatacc agcacctgaa acggcttgat aagttgaaac aatgatacgg tccaagcccc
    13621 atttttggcg aaccggctca agagccacca tcatttggat tgttgaacag ttagggcagg
    13681 caatgactcc gttgtgggca tcaagtgcat gagcattgac ctctggaaca accaaaggaa
    13741 catctggatt ttgacggaaa taagatgtat tatctactac taccacgcca gcttttactg
    13801 cgtatggtgc atacttagct gatgtagaac tacctgctga aaagagagca atatcaactc
    13861 cttcaaaagc tgtttcagtc gtttcttcaa ttgtaatatc ttgatcttta aatttcaatg
    13921 acttacctgc tgaacgtgca gaagcaaggt aacggatttt atcgattgga agtgttgatt
    13981 cttccaacat ttttatcatc tgagcaccga cagcacctgt cgcgccgact acagcaactg
    14041 tatatcccat aaataacctc tttcggaatt ttctaaaaat ttctataata gaagtattat
    14101 actacttttt ccatgaattg caaagctttt tcatcatttt ttggaaaata aaaatcccag
    14161 tcgctagact agggactaag aggcatcttc atgtgatgag caggttcaca caactcatca
    14221 aggtccgctc ctgcgttatg acctccttat gctcaatagt agtccgaaga ctacctatcg
    14281 actcatcgca tattctatat tactagagat acttgatttt gtcaataaga aattttttcg
    14341 atcgagtata acaaaaaact ctcgtattca agagtttccc tatacatatc atgctaattg
    14401 ccgggattga accggcgacc tcatccttac catggatgcg ctctgccaac tgagctaaat
    14461 cagcttactt aagaagtata ctatattcag aaaaacttgt caagtttcct tagaaatttt
    14521 ccataggaaa aagccaggta agaactacct agcttatctt cttctattta cttaaatcaa
    14581 ttcgacgacc aattttacca ataataatcg ctccaataat caatgaagca aattcaccga
    14641 ttcctgttgt aaaccaagta aggaagaatg gtaattctgc tacaatattt aactctgcag
    14701 cgatagtaaa catggaaatt gaaaagagga ttgaaaagaa gaaatgatct tttcgaatca
    14761 atccgttaaa gaggtaatcc ttgctatatt ttgcaaaaag ccaaacacct aaactgagga
    14821 atactaaggt tgaaccacca ccaacaaaga catctagcag tccgaagcta aagaaattag
    14881 caatcataca accgatggta actccgatga tgtacttagg attgtaaaaa gccataaagt
    14941 tcatcatttc tgaaatacga aactgataag caccatagct gatggcattt aggggcggtg
    15001 tgacagtcaa aacgacgtag atagcagcga cgattgcaat atctgcaaca tcacgaatag
    15061 ttaatttttt catcttttct cctttggcgg ttatccgcgt gtaatatgct tggtgaaaga
    15121 agctaagcac caagggttga ttcattcaac cttactagta tagcacaata gcctgcttta
    15181 tgctatactt aaagcatgaa aaaacctatt caaaaatttt ttaacaatga aatcttagcc
    15241 tatctctttt ttggccttgc gacaactcta gtttcgattc tctcacgact agtcatttat
    15301 caactaagtc atcaggaact ccttgctacg gccttggcaa atattatcgg tatcttattt
    15361 gcctttatca caaatgatac gattgtattt aagcaaaaaa agaaaaatag acttatccgt
    15421 ttagtgaaat tttctcttgc tcgcctttct acttttctgt tagacttgtt tttaactttt
    15481 ctctttgtaa cccaatttcc tcacatcata gggcaattcg taactgaaaa cattgataag
    15541 ataaatgcta ttgaaacaat aattgcacaa atattcatta ttatcatcaa ttatatcttg
    15601 agcaaggtct atatttttag aaaataatgc tccatttatg agccttttaa ttgccttcta
    15661 taatgattta ggatacaatg aaacacgaaa cattgaaagg aaaacagtat gttaaaaaat
    15721 ctaaaatcgt tcttgcttcg aggaaatgtt attgaccttg ctgtcggtgt tgtcattgcc
    15781 tctgcttttg gtgctatcgt tacttcactt gtaaacgaca ttatcactcc tcttatttta
    15841 aatcccgctt tgaaagctgc taaagttgaa cgtatcgctc aactttcttg gcatggagtc
    15901 ggctatggta acttcttaag tgctattatc aattttatct ttgtgggtac cgccctcttc
    15961 tttattatca agggcattga aaaagcacag aaactgactg gcataaagga agaaaaaact
    16021 gccgaaaaaa aaccaaccga attggaagtc cttcaagaaa taaaagctct ccttgagaaa
    16081 aaataatcga ttaaaaggat ctagcgcaaa gctaaatcct tttttcttta ctctttgggt
    16141 aacttgcctg ctttttcaag catttttttg ataagataag gcatcttaac attctcccgc
    16201 ccttttttct caatcagttt tttcacaaat tctggcattt gtaaatcttc agattcagcc
    16261 aaaatgtttt tcgtctcgtc tgaaggaact aactcatcaa aggtctcttc ttctgggaga
    16321 aattccttaa actcttgatt ttcattgatt ctaattgtat tgaccaaagc atcaatcaaa
    16381 cgagaatctg tatttggcat tggtggacga tggtagttta ccccaaattc ctgacataag
    16441 tcataacatt ccacatcgtt gtcaaacaag acctcaatat gctcactgat gaaactaata
    16501 ggtacaaaaa tataatggtc tggatgttct gtctgttctc tgagatactc caaaacatct
    16561 ggtttaatcc atggaatacc aatatcactc tcactctgcc aagtgttggt atattgtttt
    16621 gaactcaagc ccagtttttc agcgactagc ttgctatttt cgaaaatttg gtctatataa
    16681 ggatcaccaa agtccaaggc aaaaataggc acactatggg ctgaaaagat gactttaaag
    16741 ctgtcttgct ttacttcttc ttttaaaatc ttagcaattt catctgccca atagtttaag
    16801 agcgcttctt cctgatacca gtccttaatg accaaaaatt gaatttgttt gctttctaaa
    16861 aatttttcat agcccatgac agagtagaaa gaataatggg gctccaaaat caagcaaata
    16921 cactgttcaa ttccgtctgc ttccatttga ccgatcacat ctggaataaa gggactggaa
    16981 aacttattag caaagtagac actgtattca ttccccagcc tagcttctac caaggcaacc
    17041 tcttgacggg taattttttg cagaggcgtg cctccaattc gtacataatt atcatagaga
    17101 atctgaatct cgtggtcttg gggtctcacc ccacgacgaa tatttgtgaa aaaatcagct
    17161 acaccttcaa aggtaatctc ttctggtgaa ccgaaggtca tcattaaaat tgcttttttc
    17221 atgactgctt ccttgtgatg tttttatcaa gtttatttta tcattaattc atcgataagt
    17281 aaacgctaac atagggacat tctcaatctt ctgtcttttg tttttctctt ctttctatga
    17341 tacaatggaa aaaataaatt caaaaggagt tttttatgac ttatccaaat ctcttggacc
    17401 gcttcttaac ctacgttaag gtcaacacgc gctctgatga acactctact actactccaa
    17461 gtacacagag tcaggttgac ttcgcaacaa atgtcctaat tcctgaaatg aaacgtgttg
    17521 gactgcaaaa tgtttactat ctaccgaatg gttttgctat tggaaccttg ccagccaacg
    17581 atccgtcttt aacacgtaag attggtttta tatcgcacat ggatactgct gattttaatg
    17641 ctgaaggagt caatccacag gtaattgaaa actacgatgg tggtgtgatt gaactaggga
    17701 attctggttt caaactcgat ccagctgact tcaagagtct tgaaaaatat ccaggacaaa
    17761 cgctcatcac aacagatgga acaaccttgc taggtgctga tgacaagtca ggaattgctg
    17821 aaattatgac agccattgaa tatctaactg ctcatcctga aattaagcac tgtgagattc
    17881 gtgttggttt tggtccagat gaagaaatcg gtgttggtgc caataaattt gatgcagaag
    17941 attttgatgt ggattttgcc tacactgttg atggtggtcc actaggtgaa cttcagtacg
    18001 agactttctc agccgctggt gctgaattgc atttccaagg ccgtaatgtc caccctggta
    18061 ctgccaaagg gcagatggtc aatgcccttc agctagcaat tgattttcat aatcaacttc
    18121 cagaaaatga ccgacctgag ttaactgaag gttaccaagg tttttaccat ctaatggatg
    18181 tgacaggtag tgttgaggag gcgcgtgcaa gctacatcat tcgtgatttt gaaaaagatg
    18241 cctttgaagc gcgtaaagca tccatgcaat ctatcgctga taagatgaat gaagaacttg
    18301 ggagcaaccg tgtcactctc aacttgacag accagtacta caatatgaaa gaagtcattg
    18361 aaaaagatat gactccaatt accattgcta aagccgttat ggaagatcta ggtatcacgc
    18421 ctattatcga accaatccga ggtggaacag acggctctaa gatttccttt atgggaatcc
    18481 caactccgaa tatctttgca ggtggcgaaa atatgcacgg acgttttgaa tacgttagcc
    18541 ttcagactat ggaacgtgca gttgatacca tcattggcat cgtacgtagc ttataaaagc
    18601 taaaaagacg aggtagctca gctacttcgc ctttcttttt attctactgg tttttcttga
    18661 tttccagtag ttgtagaaga ttctgttgtt ttattttctg aagttgattc agcaggttta
    18721 gaatctcttg tattgcttgg tttgttttcg tcgctagcag tttcaatgtt agattctgca
    18781 gttgcgtttg gttggttctc agcactggtg ttatcaccat ttgcttcagc atttcttgct
    18841 ggacttgttt cttcacttgc gctagctttt gactggattt gatgattcaa aactagaata
    18901 gcttttgtcg attcaagtaa agctgttttg tctttactct tagcagaaag ttgatctaat
    18961 aatgcatcca ccttatcaaa gtccgcatca gatccattat tactttctaa ataagagtga
    19021 agcgacatga gaatatcgta gagtttttga tagagtacaa gtgtctgagg atcttgctca
    19081 gcattttcct tttcttgttg aagggcgcta gcgatacgag tcaaaacatc ttttacctga
    19141 ctgtttactt catccaagtc tgcatcagcc ttgtttgtgg cagcttttag attttctact
    19201 tcttctgcca aggattgtct gattccttct tcatggattt gttccaagag ttgatttgcc
    19261 ttgttcaaaa gactttctac ttcttccttg ctatctgtcg cagattattg gttgctatct
    19321 accatgtact cctaaaacag gagagttata atccaagatt acaaggcctt acagaaataa
    19381 gaaatccaga taagacaatg ttcgtccaag acgctattcg cttcgcacag cagcacggat
    19441 tcaatatgct ttaattttaa agtttaggtg tcaagacctc tttttagtgt gcccaaaatt
    19501 tagagaagta atcaatcaac taacttttat ttttttcaaa ctttcagtaa actgacctaa
    19561 agctaactca atctgtcttt gtagatgctt ctgctatcag ctagaagttg atctactttt
    19621 gccaagactg ccttctcatc aaaagttcca ggttgatagt tggattgcag ggatggaatc
    19681 ttgtttttca aagccgcttc atatccctta gtttgaacct tgatgtagtg attgtggtcg
    19741 ccatgaggaa tcacaaaacc ttctgaatct tcacttataa ttcgattggc atcaaaaacc
    19801 atgaccatct tcttcctcat ggtggacatg tagtgacgga ttacttaata cagaactaga
    19861 agaacttcct acctcttccg tgttagagtg tgatggggga ttgttaagag atgacttagg
    19921 aatatagtga tagtgttccc catgtcttac tatataagca tcacctatat ctctgacaat
    19981 atcattaggg ttaaagacat atgtggctgc taattcacct gccgacaagt cactctcagg
    20041 aatgaaatga tagtgaccac catgtggtac tatagtaggt tgaaatagaa tatgagcaaa
    20101 ttgataaggg gattttaaag taatttctaa caatgattta gaaactatga tgtgctattc
    20161 taaattcaac tcactatata taaccatcat cggtagtata acgtccctgt aattttgcta
    20221 cagatacttc tgcactagct cctttatcgt ctttaccatg ttcttgtttt tggcgattga
    20281 tttcatcttt tgttcgtaca ttttctgcat gagcttgatc tttaaggtaa acataatact
    20341 ttccatctac cttaataata tatcctccct taacctaact gacgatatct tgatctttcg
    20401 gctgatagtt gggggctttc attagtagct cttcactaaa gagcgcatca aaaggaactt
    20461 taccattata gtagtgataa tgatcgccat gagaagttac ataaccttga tctgtaatct
    20521 taataacaat ttgttttgct tgaattcctt ctttttgact aacctagtct ggagtcaaat
    20581 tttcagtctt cttagtgtct ttattactgt ttacatatga aacacgattt ttatctgtat
    20641 tggcctgtta gctatgttgg ttcagagcat aaacacacag acttaaggaa aggataacaa
    20701 cagat
//
LOCUS       DAVKJD010000031        18372 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKJD010000031 DAVKJD010000000
VERSION     DAVKJD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.031"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            113..5416
                     /locus_tag="VW157_001139"
     CDS             113..5416
                     /locus_tag="VW157_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9552447.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVANKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKIPGNSTWYKGNRVAAPETGSINRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHINASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREASDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVAED
                     FASLDAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKSKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(5583..7742)
                     /locus_tag="VW157_001140"
     CDS             complement(5583..7742)
                     /locus_tag="VW157_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEU9552448.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(7739..8335)
                     /gene="recU"
                     /locus_tag="VW157_001141"
     CDS             complement(7739..8335)
                     /gene="recU"
                     /locus_tag="VW157_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9552449.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            8401..8928
                     /locus_tag="VW157_001142"
     CDS             8401..8928
                     /locus_tag="VW157_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9552450.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            8998..9327
                     /gene="gpsB"
                     /locus_tag="VW157_001143"
     CDS             8998..9327
                     /gene="gpsB"
                     /locus_tag="VW157_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9552451.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            9348..9728
                     /gene="rnpB"
                     /locus_tag="VW157_001144"
     ncRNA           9348..9728
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW157_001144"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9813..10970
                     /locus_tag="VW157_001145"
     CDS             9813..10970
                     /locus_tag="VW157_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9552452.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPM
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            10983..12377
                     /gene="mapZ"
                     /locus_tag="VW157_001146"
     CDS             10983..12377
                     /gene="mapZ"
                     /locus_tag="VW157_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9552453.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVKKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            12453..13877
                     /gene="gndA"
                     /locus_tag="VW157_001147"
     CDS             12453..13877
                     /gene="gndA"
                     /locus_tag="VW157_001147"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9552454.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            13889..14578
                     /locus_tag="VW157_001148"
     CDS             13889..14578
                     /locus_tag="VW157_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9552455.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            14676..15701
                     /gene="cbpC"
                     /locus_tag="VW157_001149"
     CDS             14676..15701
                     /gene="cbpC"
                     /locus_tag="VW157_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU9552456.1"
                     /translation="MKILKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDGGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDPATGIM
                     QTGWQFLDNRWYYLRSSGAMATGWYQEGSTWYYLDQSNGDMRTGWQYLGNKWYYLRSS
                     GAMVTGWYQDGSTWYYLDPSNGDMKTGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNF
                     ASSGEWI"
     gene            15720..16595
                     /locus_tag="VW157_001150"
     CDS             15720..16595
                     /locus_tag="VW157_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9552457.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMRTGWQYL
                     GNNWYYLRSSGAMATGWYQDGSTWYYLNVGNGDMKTGWFQVNGNWYYAYSSGALAVNT
                     TVDGYSVNYNGEWVR"
     gene            complement(16713..17945)
                     /locus_tag="VW157_001151"
     CDS             complement(16713..17945)
                     /locus_tag="VW157_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9552458.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLASIFLYL
                     ILIALIFCQWLVTFNTHN"
BASE COUNT         5668 a         3332 c         4229 g         5143 t
ORIGIN      
        1 agtataacta ttttttattt atcagggtta agcattgcac tttcagagga aggagtattt
       61 tttaaaaagg aaatgtaaac gcttgcccaa aaatgaaagg atttagaagt ttatgaataa
      121 aggattattt gaaaaacgtt gtaaatatag tattcggaaa ttttcattag gtgttgcttc
      181 tgttatgatt ggagctgcat tctttgggac aagtccggtt cttgcagata gcgtgcagtc
      241 tggttccacg gcgaacttac cagctgattt agctactgct cttgcaacag caaaagagaa
      301 tgatgggcgt gattttgaag cgcctaaggt gggagaagac caaggttctc cagaagttac
      361 agatggacct aagacagaag aagaactatt agcacttgaa aaagaaaaac cagctgaaga
      421 aaaaccaaaa gaggataaac ctgcagctgc taaacctgaa acacctaaga cggtaacccc
      481 tgaatggcaa acggtagcga ataaagagca acagggaaca gtcactatcc gagaagaaaa
      541 aggtgtccgc tacaaccaac tatcctcaac tgctcaaaat gataacgcag gcaaaccagc
      601 cctgtttgaa aagaagggct tgaccgttga tgccaatgga aatgcaactg ttgatttaac
      661 cttcaaagaa gattctgaaa agggcaaatc acgctttggt gtcttcctga aatttaaaga
      721 taccaataat aatgtttttg tcggttatga caaggatggc tggttctggg aatacaaaat
      781 tccaggtaat agcacatggt ataaaggcaa ccgtgttgct gctcctgaaa caggatcaat
      841 aaaccgtctt tctatcactc tcaagtcaga cggtcagcta aatgccagca ataacgatgt
      901 caatctcttt gacacagtga ctctaccagc tgcggtcaat gaccatctta aaaatgagaa
      961 gaagattctt ctcaaggcgg gctcttatga cgatgagcga acagttgtta gcgttaaaac
     1021 ggataaccaa gagggggtaa aaacagagga tacccctgct gaaaaagaaa caggtcctga
     1081 agttgatgat agcaaggtga cttatgacac gattcagtct aaggtcctca aagcagtgat
     1141 tgaccaagcc ttccctcgtg tcaaggaata cagcttgaac gggcatactt tgccaggaca
     1201 ggtgcaacag ttcaaccaag tctttatcaa taaccaccga atcacccctg aagtcactta
     1261 taagaaaatc aatgagacaa cagcagagta cttgatgaag attcgcgatg atgctcactt
     1321 aatcaatgcg gaaatgacag tacgcttgca agttgtggac aatcaattgc actttgatgt
     1381 gaccaagatt gtcaaccaca atcaagtcac tccaggtcaa aagattgatg acgaaagaaa
     1441 actactttct tctattagtt tcctcggcaa tgctttagtc tctgtttcta gtgatcaaac
     1501 tggtgctaag tttgatgggg caaccatgtc aaacaatacg catgtcagcg gagatgatca
     1561 tatcgatgta accaatccaa tgaaagacct agccaagggt tacatgtatg gatttgtttc
     1621 tacagataag cttgctgctg gtgtttggag taactctcaa aatagctatg gtggtggttc
     1681 gaatgactgg actcgtttga cagcttataa agaaacagtc ggaaatgcca actatgtagg
     1741 aatccacagc tctgaatggc aatgggaaaa agcttataag ggcattgttt tcccagaata
     1801 cacgaaggaa cttccaagtg ctaaggttgt tatcactgaa gatgccaatg cagacaagaa
     1861 agtcgattgg caagatggtg ccattgctta tcgtagcatt atgaacaacc ctcaaggttg
     1921 ggaaaaagtt aaggatatca cagcttaccg tatcgcgatg aactttggtt ctcaagcaca
     1981 aaacccattc cttatgacct tggatggtat caagaaaatc aatctccaca cagatggtct
     2041 tgggcaaggt gttctcctta aaggatatgg tagcgaaggt catgactctg gtcacttgaa
     2101 ctatgctgat attggtaagc gtatcggtgg tgtcgaagac ttcaagaccc taattgagaa
     2161 ggctaagaaa tatggagctc atctaggtat ccacattaac gcttcagaaa cttatcctga
     2221 gtctaaatac ttcaatgaaa aaattctccg taagaatcca gatggaagct atagctatgg
     2281 ttggaactgg ctagatcaag gtatcaacat tgatgctgcc tatgacctag ctcatggtcg
     2341 tttggcacgt tgggaagatt tgaagaaaaa acttggtgac ggtctcgact ttatctatgt
     2401 ggacgtttgg ggtaatggtc aatcaggtga taacggtgcc tgggctaccc acgttcttgc
     2461 taaagaaatt aacaaacaag gctggcgctt tgcgatcgag tggggccatg gtggtgagta
     2521 cgactctacc ttccatcact gggcagctga cttgacctac ggtggctaca ccaataaagg
     2581 tatcaacagt gccatcaccc gctttatccg taaccaccaa aaagatgctt gggttgggga
     2641 ctacagaagt tatggtggtg cagccaacta tccactgcta ggtggctaca gcatgaaaga
     2701 ctttgaaggc tggcaaggaa gaagtgacta caatggctat gtaaccaact tatttgccca
     2761 tgacgtcatg actaagtact tccaacactt cactgtaagt aaatgggaaa atggtacacc
     2821 ggtgactatg accgataacg gtagcaccta taaatggact ccagaaatgc gagtggaatt
     2881 ggtagatgct gacaataata aagtagttgt aactcgtaag tcaaatgatg tcaatagccc
     2941 acaatatcgc gaacgtacag taactctcaa cggacgtgtc atccaagatg gttcagctta
     3001 cttgactcct tggaactggg atgcaaatgg taagaaactt tctactgata aggaaaagat
     3061 gtactacttc aatacgcagg ccggtgcaac aacttggacc cttccaagcg attgggcaaa
     3121 gagcaaggtt tacctttaca agctaactga ccaaggtaag acagaagagc aagaactaac
     3181 tgtaaaagat ggtaaaatta ccctagatct tctagcaaat caaccatatg ttctctatcg
     3241 ttcgaaacaa actaatcctg aaatgtcatg gagtgaaggc atgcacatct atgaccaagg
     3301 atttaatagc ggtaccttga aacattggac catttcaggc gatgcttcta aggcagaaat
     3361 tgtcaagtct caaggggcaa acgatatgct tcgtattcaa ggaaacaaag aaaaagttag
     3421 tctcactcag aaattaactg gcttgaaacc aaataccaag tatgccgttt atgttggtgt
     3481 agataaccgt agtaatgcca aggcaagtat cactgtgaat actggtgaaa aagaagtgac
     3541 tacttatacc aataagtctc tcgcgctcaa ctatgttaag gcctacgccc acaatacacg
     3601 tcgtgacaat gctacagttg acgatacaag ttacttccaa aacatgtacg ctttctttac
     3661 aactggatcg gacgtctcaa atgttactct gacattgagt cgtgaagcta gtgatcaagc
     3721 aacttacttt gatgaaattc gtacctttga aaacaattca agcatgtacg gagacaagca
     3781 tgatacaggt aaaggcacct tcaagcaaga ctttgaaaat gttgctcagg gtatcttccc
     3841 atttgtagtg ggtggtgtcg aaggtgttga agataaccgc actcacttgt ctgaaaaaca
     3901 cgatccatat acacaacgtg gttggaatgg taagaaagtc gatgatgtta tcgaaggaaa
     3961 ttggtcactc aagacaaatg gactagtgag ccgtcgtaac ttggtttacc aaactattcc
     4021 gcaaaacttc cgttttgaag caggtaagac ctaccgtgta acctttgaat acgaagcagg
     4081 ttcagacaat acatatgctt ttgtagtcgg taagggagaa ttccagtcag gtcgtcgtgg
     4141 tactcaagca agcaacttgg aaatgcatga attgccaaat acttggacag attctaagaa
     4201 agccaagaag gcaaccttcc tcgtgacagg tgcagaaaca ggggatactt gggtaggtat
     4261 ctactcaact ggaaatgcaa gtaatactcg tggtgattct ggtggaaatg ccaacttccg
     4321 tggttataac gacttcatga tggataatct tcaaatcgaa gaaattaccc taacaggtaa
     4381 gatgttgaca gaaaatgctc tgaagaacta cttgccaacg gttgccatga ctaactacac
     4441 caaagagtct atggatgctt tgaaagaggc ggtctttaac ctcagtcagg ccgatgatga
     4501 tatcagtgtg gaagaagcgc gtgcagagat tgccaagatt gaagccttga agaatgcttt
     4561 ggttcagaag aaaacggctt tggtagcaga agactttgca agtttggatg cgccagctca
     4621 ggctcaagaa ggtcttgcaa atgcctttga tggaaactta tctagtttat ggcatacatc
     4681 atggggcgga ggagatgtag gcaagcctgc aaccatggtc ttgaaagaag caactgaaat
     4741 cactggactt cgttatgttc cacgtggatc aggttcaaat ggtaacttgc gtgatgtgaa
     4801 acttgttgtg acagatgagt ctggcaagga gcataccttt actgcaactg attggccaga
     4861 taacaataag tcaaaagaca ttgattttgg taagacaatc aaggctaaga aaattgtcct
     4921 tactggtacc aagacatacg gagatggtgg cgataaatac caatctgcag cggaacttat
     4981 ctttactcgt ccacaggtag cagaaacacc tcttgacttg tcaggctatg aagcagcttt
     5041 ggctaaggct cagaaattaa cagacaaaga caatcaagag gaagtagcta gcgttcaggc
     5101 aagcatgaaa tatgcgacgg ataaccatct cttgacggaa agaatggtgg aatactttgc
     5161 agattatctc aaccaattaa aagattctgc tacgaaacca gatgctccaa ctgtagagaa
     5221 acctgagttt aaacttagct ctttagcttc cgatcaaggt aagacgccag attataagca
     5281 agaaatagct agaccagaaa cacctgaaca aatcttgcca gcaacaggtg agagtcaatc
     5341 tgacacagcc ctcttcctag caggtgttag cctagcccta tctgctctct ttgtagtaaa
     5401 aacgaagaaa gactagtatt tagtaaaacc tcttaacaag attacggaag cagtctctat
     5461 cttttccaat gaggtttata gtacagaaaa agtctgagaa gatgtcttct cagacttttg
     5521 ttaagcacat aaatacaata gtgctatgac aaaatcaccc agaaaaatct gggtgataaa
     5581 tgttatggtt gtgctggttg aggattctga ttttgttgat caggggttgt atttgattgt
     5641 tgcgtattat tgttaggatt ggtagtcgta ctattatttg tgcttggagt ggttgagcta
     5701 gactgtgaag ttgaactatc tgatgatgag cttgaacttt cagttgatgg gggttgttgt
     5761 ggagcaggtg agttccacgt agaacgagca ccatttttaa atacgaattc tccatttctg
     5821 tagagcccct ctggtatatt ccaatcttct ggattgcttc cttcagacag gtaggtcatc
     5881 atagagcggt aaactttggc agcgaccgta aggccattgc ctacaagtgg tgtcagacgg
     5941 ttagaatagc ctgtccatac agccattgaa tatttacgcg tatagccagc aaatagttca
     6001 tcaggtgcta caaattgaga ggtcttgatg tggttttcaa tttcctcgtc tgtatagtta
     6061 gaggttcctg ttttaccagc ctgagggagc caagcaagat aggcatttcg tccagttcca
     6121 taagtcaaga ctgttttcat catgtcggtc atcatatagg ctgtcgtttc tttcatggca
     6181 cgagttccga cattagagaa ctctttttca ctcccatcac taaagacgac tttatggata
     6241 tacattggtt tatagtaagt tccaccattt gcaaaggcag cgtaagcagc agccatcttt
     6301 tcactacttg ctccatattt tttgtctgat tcggttgtgt tacttgaaat ggcatttgag
     6361 tagtgaatac ttgggtagtc gattccgaga ccatttagga aagtcttggc gcggttgagt
     6421 ccgaccttgt ttagagtttc cacggctggg acgtttcgcg attgttgcag ggcgtattgc
     6481 aaggtgatgt tgccaaagta gcccctatcc cagttataaa caggggtatt tgtcccaggg
     6541 tagttatagg gctcatcgtg aacgatagta gcagttgaat cgtagacacc gtactccaag
     6601 gcaggagcat agtctgtgat cggtttcata gttgatcccc agtcgcggtt tgtttctact
     6661 gcttggttaa ttccgaagga aacattactt gactgatggc gtgctcctag ctgggcaatg
     6721 actttaccgt tagaaacatc aacaatggta gaagcgactt gcaattcatc gtctggatag
     6781 gcaacgtatt cgtctgtatt gtaaatatcc cacagatgtt tttgagcttc ttggtctaca
     6841 tttgtgtaga catccattcc agttgtgagc aggttatagc ctgtttcttc ttcaacttga
     6901 ttgatgactt ccttgaggta attatccatg taagcagggt aattacttgc tgatttgaga
     6961 ctttgtagtc catcagtaat tggtgtattg actgctttct catactgttc agcagagatg
     7021 tagccttgat ttttcatttc agataagacc aagtttcggc ggtcttgggc tgcttctgga
     7081 tgtgaatagg ggtcatattg gtttggtgcc tgaggcattc cagccagcaa ggctaactga
     7141 ggtaaactta aattattgag gtctttacca tagtagtttt gagctgctgt ctgcattcca
     7201 tagttcccat tagacatgta gaccttattt atatagtagg tcaagatttc ttgcttggtt
     7261 gctttttgtt ctaactgaat cgctaaccaa gcttcctgag ccttacgaga aatagtctgg
     7321 tcggaagtcg aagttgaaaa gtaagtcaac ttaatcaact gttgggtgag agttgatcca
     7381 ccttggaggg aattgctttg cagattgcgc aagaaagctc ctaggatacg gatggtatca
     7441 atccccctgt ggtcgaagaa gcgatggtct tcgatagaaa cgattgcctt aaccaaatct
     7501 gtgggaatat cattagcttg ggcattgacg cggcgttcag aacccaagtc agcaatgagt
     7561 tgatttttat tgtcgtagat tttactagaa gttgttgcaa ctagtttact ctcggatagg
     7621 ctaggagcct tgctaacgta gtagaaaaaa actcctccgc ctaagacaat ggctgcgata
     7681 accaagctta agaagctaat gctcagatac ttgattaggc gcagaatcgt tggtttgttc
     7741 atcttgtttt accacctaat aaatgttctt tgataacatt gagataagga atttgaggga
     7801 aggcaccagc cttgatttca tatccatatt ctcgaatata ttcaagtggc attgattttt
     7861 gtcccttatc ttgatgatag aagcgaatca aatcgaatgc cggcaataag taggtttctt
     7921 gctgagaaga aaagtgaaga aggacaaagc agattccttg ttgggcaagg acttgttcca
     7981 tatgctgaat ctgatgtgga tgaaaatttt tcatcggaat cgcacgtttt tgttttgttt
     8041 ccttgacttc aaagtcgatg taatatccat tataaacgcc agaatagtcc gtcgttgaag
     8101 cttgtcgaaa ataggcttca acaatcttgg cacgacttcg ttgtggatag tccacttgta
     8161 cgatttgaat aggagttggt ttcttatgta taacagccaa gccctgagac aaatagtagt
     8221 cgttggtagc attgatcatc ttttcaaaag acattcctcg atttgcgaaa tttttgggtt
     8281 gagaaagaga tgtttgtctt ttttgtgatg aaactttatg tggatagttg accataattc
     8341 tccttattgg tacaataaca tcactttatt ataccataat ttgcacagaa agggttaaaa
     8401 atggctacag ctttggtttt aggctattct gcttttgatt tgggtttatt ttctgataag
     8461 gatcctcgtc ttaaattgat taaaaaagcg atacgtaaag atttagaggc tatggcagca
     8521 gatggggtgt cttggcttgt gtttacagga agtttggggt ttgaatattg ggttttagag
     8581 gttgctcagg agatgaaaac cgaatacggt ttccagttgg ccaccatttt tgcttttgaa
     8641 acccatgggg aaaattggaa tgaaggcaat cagatgaaac tgagtcgctt taagcaggta
     8701 gactttgtca aatatgccta tcctcgctat gaacacaagg ggcagttaag agattaccag
     8761 cagtttttgc tggaaaacac gactagttcc tatctttttt atgatgaaga aaatgaaaca
     8821 aaactagctt atttttacca aaagatgaaa aatcaagagg actattttat aaagagatta
     8881 acatttgatc aattaaatga acttgctgaa aatttttccg aaaattgaag ctttgacctt
     8941 gatttttgtt tgcctttttt tatataataa tactagcaag cgagaatgga gagagacatg
     9001 gcaagtatta ttttttcagc gaaagatatt tttgaacaag agtttggacg tgaagtccgt
     9061 ggctataata aagtagaagt tgacgagttt ttagacgatg tcatcaagga ctatgaaacc
     9121 tatgctgcct tggtcaagtc acttcgtcag gaaattgcgg atttgaagga agaattaact
     9181 cgtaaaccga aaccttcacc agttcaagca gaaccccttg aagcggcaat tacaagttct
     9241 atgacgaatt ttgatatttt gaaacgcctg aatagattgg aaaaagaagt ttttggtaaa
     9301 caaattttag ataactcaga tttttaagta gttatttgag atgtgcaatt tttggataat
     9361 cgcgtgagga gaattgcttc tcatgaggaa agtccatgct agcacaggct gtgatgcctg
     9421 tagtgtttgt gctaggcgaa accataagcc tagggacgag aaatcgttac ggcagttgaa
     9481 atggctaagt ccttggataa gccagagtag gcttgaaagt gccacagtga cggagtcttt
     9541 ctggaaacag agagagtgga acgcggtaaa cccctcaagc tagcaaccca aattttggtc
     9601 ggggcatgga gtacgcggaa acgaacgtag tattctgact gctatcagct agagctgtta
     9661 gtggtagaca gatgattatc gaaggaagtg gtcctagtca cttctggaac aaaacatggc
     9721 ttatagaaaa ttgcatatag gttggggctg agaaattttc tcaacctcat tttttaaagt
     9781 ggacatatag aaaggtcttg caagactgta acatgaaaaa agaatttaat ttaattgcaa
     9841 ctgtggcagc agggcttgag gctgtcgttg gtcgtgaagt gcgagagttg ggctacgatt
     9901 gtcaggttga aaatggacgt gttcgttttc aaggagacgt gagagctatt atcgaaacca
     9961 acctttggct tcgggcagca gatcgtatca aaattatcgt aggaacgttc ccagctaaga
    10021 cttttgaaga gctatttcag ggagttttcg ctttagattg ggaaaattat ttaccacttg
    10081 gagctcggtt cccgatttca aaagctaaat gtgttaagtc caaacttcac aatgagccta
    10141 gtgttcaggc tatttctaag aaagctgttg tcaagaaatt gcagaaacac tatgctcgcc
    10201 cagaaggggt tcctctgatg gagaatggcc cagagtttaa gattgaggtc tctattctca
    10261 aagatgtggc aactgtcatg attgatacga ccgggtctag cctctttaaa cgtggttatc
    10321 gtaccgaaaa aggtggcgct cctatcaagg aaaatatggc agcagccatt ttacaacttt
    10381 ctaactggta cccagacaag cctttgattg atccgacctg tggttcgggg actttctgta
    10441 ttgaggcagt tatgattgct agaaagatgg cgccaggtct tcgtcgctct tttgcatttg
    10501 aggaatggaa ctggatcagc gatcgcttga ttcaagaagt gcgcacagaa gcggctaaaa
    10561 aagtagaacg tgagcttgag ctggatatca tgggctgtga tattgatgct cgcatggtgg
    10621 aaattgctaa ggccaatgct caggcagctg gtgttgcagg agacattact tttaagcaga
    10681 tgcgcgtgca ggatttacgt tccgataaaa tcaatggagt aatcatttcc aatccgcctt
    10741 atggtgaacg tttgtcagat gatgcagggg tgaccaagct ctatgctgag atggggcaag
    10801 tatttgcacc gatgaaaact tggagcaaat ttatcctgac tagtgatgaa gcttttgaaa
    10861 gcaagtatgg tagccaagca gataagaagc gtaagttata caacggaacc ttgaaagtgg
    10921 atctgtatca atattttggt cagcgtgtca aacggcaaga ggtaaaatag aaagggatac
    10981 tcatgagtaa aaaaagacga aatcgtcata aaaaagaagg tcaagaaccg caatttgatt
    11041 ttgatgaagc aaaagagcta acagttggtc aagctattcg taaaaatgaa gaagtggaag
    11101 caggagtctt gcctgaggat tccattttgg acaagtatgt taagcaacac agagatgaaa
    11161 ttgaggcgga taagtttgcg actcgtcaat acaaaaaaga ggagttcgtt gaaactcaga
    11221 gtctggatga tttaattcaa gagatgcgtg aggctgtaaa gaagtcagaa gcttcttcgg
    11281 aggaagttcc atcttctgaa gacatcttac tacccttgcc tctggacgat gaggagcaag
    11341 gcttggatcc tctattgcta gatgatgaaa atccaacaga aatgactgaa gaagtggaag
    11401 aggagcaaaa cctttctcgt ctggatcaag aggactcaga aaagaaaagt aaaaaaggct
    11461 ttattttgac cgttttggcg cttgtatcag taattatttg tgtcagtgct tattatgtct
    11521 accgtcaagt ggctcgttcg actaaggaaa ttgaaacttc tcaatcaact acagccaatc
    11581 aatcggatgt ggatgatttt aatacacttt atgacgcctt ttacacagat agcaataaaa
    11641 cggctttgaa aaatagccag tttgataaac tgagtcaact caaaaccttg cttgataaac
    11701 tggaaggtag tcgtgaacat acgcttgcca aatctaaata tgatagtcta gcaacgcaaa
    11761 tcaaggctat tcaagatgtc aatgcacaat ttgaaaaacc agctattgtg gatggtgtgt
    11821 tggataccaa tgccaaagcc aaatcggatg ctaaatttac ggatattaaa actggaaata
    11881 cggagcttga taaagtgcta gataaggcta tcagtcttgg taagagccag caaacaagta
    11941 cttctagctc aagttcaagt caaactagca gctcaagttc aagtcaagca agttcaaata
    12001 cgactagtga gccaaaacca agtagttcaa atgagactag aagtagtcgc agtgaagtca
    12061 atatgggtct ctcgagtgca ggggttgctg ttcaaagaag tgccagtcgt gttgcctata
    12121 atcagtctgc tattgatgat agtaataact ctgcctggga ttttgcggat ggtgtcttgg
    12181 aacaaattct agcgacttca cgttcacgtg gctatatcac tggagaccaa tatatccttg
    12241 aacgtgtcaa tatcgttaac ggcaatggtt attacaacct ctataagcca gatggaacct
    12301 atctctttac ccttaactgt aagacaggct actttgtcgg aaatggcgct ggtcatgcgg
    12361 atgacttgga ctactaagca gtcgttacaa aattctttct tttcaaaagt aaaaatgata
    12421 aaataaaaca aatgaaacaa gaggagtgtc aaatgacaaa agctaacttt ggtgtcgtag
    12481 gtatggccgt aatgggtcgt aaccttgccc ttaatattga atcacgtggt tacacagttg
    12541 ctatctacaa ccgtagtaaa gaaaaaacgg aagatgtgat tgcttgccat cctgaaaaga
    12601 actttgtacc aagctatgac gttgaaagtt ttgtaaactc aatcgaaaaa cctcgtcgta
    12661 tcatgctgat ggttcaagct ggacctggta cagatgctac tatccaagcc cttcttccgc
    12721 accttgacaa gggtgatatc ttgattgacg gaggaaacac tttctacaaa gataccatcc
    12781 gtcgtaatga agaattggca aactcaggta tcaactttat cggtactgga gtttctggtg
    12841 gtgaaaaagg tgcccttgaa ggtccttcta tcatgcctgg tggacaaaaa gaggcctacg
    12901 aattggttgc ggatgttctt gaagaaatct cagctaaagc accagaagat ggcaaaccat
    12961 gtgtgactta catcggccct gatggagctg gtcactatgt gaaaatggtt cacaatggta
    13021 ttgagtacgg tgatatgcaa ttgatcgcag aaagctatga cttgatgcaa cacttgctag
    13081 gtctttctgc agaggatatg gctgaaatct ttactgagtg gaacaagggt gaattggata
    13141 gctacttgat cgaaatcaca gctgatatct tgagccgtaa agacgatgaa ggccaagatg
    13201 gaccaatcgt agactacatc cttgatgctg caggtaacaa gggaactggt aaatggacga
    13261 gccaatcatc tcttgacctt ggtgtaccat tgtcactgat tactgagtca gtgtttgcac
    13321 gctacatttc aacttacaaa gaagaacgtg tacatgctag caaggtgctt ccaaaaccag
    13381 ctgccttcaa ctttgaagga gacaaggctg aattgattga aaagatccgt caagcccttt
    13441 acttctcaaa aatcatttca tacgcacaag gatttgctca attgcgtgta gcctctaaag
    13501 aaaacaactg gaacttgcca tttgcagata tcgcatctat ctggcgtgat ggctgtatca
    13561 tccgttctcg tttcttgcaa aagattacag atgcttacaa ccgcgatgca gatcttgcca
    13621 accttctttt ggacgagtac ttcttggatg ttactgctaa gtatcaacaa gcagtgcgtg
    13681 atatcgtagc tcttgcggtt caagctggtg tgccagtgcc aactttctca gcagctatta
    13741 cttactttga tagctaccgt tcagctgacc ttccggctaa cttgatccaa gcacaacgtg
    13801 actactttgg tgctcacact taccaacgta aagacaaaga aggaaccttc cactactctt
    13861 ggtatgacga aaaataagta ggtcagccat ggggaaacgg attttattac ttgagaaaga
    13921 acgaaatcta gctcattttt taagtttgga actccagaaa gagcagtatc gggttgatct
    13981 ggtagaggag gggcaaaaag ccctctccat ggctcttcag acagactatg atttgatttt
    14041 attgaacgtt aatctgggag atatgatggc tcaggatttt gcagaaaaat tgagtcgaac
    14101 taaacctgcc tcagtcatca tgattttaga tcattgggaa gacttgcaag aagagctgga
    14161 agttgttcag cgttttgcag tttcatacat ctataagcca gtccttatcg aaaatctggt
    14221 agcgcgtatt tcggcgatct tccgaggtcg ggacttcatt gatcaacact gcagtctgat
    14281 gaaagttcca aggacctacc gcaatcttag gatagatgtt gaacatcaca cggtttatcg
    14341 tggtgaagag atgattgctc tgacccgccg tgagtatgac cttttggcga cacttatggg
    14401 aagcaagaaa gtattgactc gtgagcaatt gttggaaagt gtttggaagt atgaaagtgc
    14461 gaccgagaca aatatcgtag atgtctatat ccgctatcta cggagcaagc ttgatgttaa
    14521 aggccaaaaa agctacatta aaaccgtgcg tggtgttggt tacaccatgc aagaatagaa
    14581 aagcagttgc agttgtgtaa ctgctttttt gaggagtttt tatatattga catgcagtcg
    14641 ggtctttgct acaatcgttt taggagggta tatagatgaa aattttgaaa aaaatgatgc
    14701 aagttctact agcagtcttt ttctttggtt tgctagctac aaatacggta tttgcgaata
    14761 ccacaggtgg ccgatttgtt gataaggata atagaaaata ttatgtaaaa gatgatcata
    14821 aagcaatcta ttggcataaa atagacggta aaacctacta ttttggtgat ggaggagaga
    14881 tggttgtcgg ttggcaatac ttagaaattc ctggaacagg ttatcgtgat aatttattcg
    14941 ataaccaacc agttaatgaa attggccttc aggagaagtg gtactatttt ggacaagatg
    15001 gtgctttgtt agaacaaaca gataaacaag tactagaggc aaaaacgtct gaaaatacag
    15061 gaaaagtata cggtgaacaa tatcctctat ctgctgaaaa gagaacttat tattttgata
    15121 ataattatgc tgtaaagaca ggctggattt atgaagacgg caattggtat tatttaaata
    15181 agctaggaaa ttttggcgat gattcttaca atccactacc aattggtgaa gttgctaagg
    15241 gttggactca agattttcat gttactattg acattgatag aagcaaacct gctccatggt
    15301 actacctaga tccagcaact ggcatcatgc aaactggttg gcaatttcta gataatagat
    15361 ggtactacct ccgttcatca ggagcaatgg caactggctg gtatcaggaa ggctcaactt
    15421 ggtattattt agaccagtca aatggtgata tgagaactgg ctggcaatat cttggtaaca
    15481 agtggtacta cctccgttca tcaggagcta tggtaactgg ttggtatcaa gatggttcaa
    15541 cttggtacta tttagatcct tctaatggag atatgaaaac aggttggaca aaagtaaatg
    15601 gaaaatggta ttatctcaat tcaaatggag caatggttac aggtagtcaa actatcgatg
    15661 gtaaagttta taacttcgct tcatctggtg agtggattta atgttggagg atatataaaa
    15721 tgaagctttt gaaaaaaact atgcaagctg gactaacagt aattttcttt ggtttgctag
    15781 ctacgaatac agtatttgca gataactctg aaggatggca gtttgtccaa gaaaacggta
    15841 gaacctatta caaaaagggg gacctcaaag aaacctactg gcgagtgatt gatggtaagt
    15901 actattattt tgattctcta tctggagaga tggttgtcgg ctggcaatat atcccgtttc
    15961 catctaaagg tagtacaatt ggtccttacc caaatggtat cagattagaa ggttttccaa
    16021 agtcagagtg gtactacttc gataaaaatg gagtgctaca agagtttgtt ggttggaaaa
    16081 cattagagat taaaactaaa gacagtgttg gaagaaagta cggggaaaaa cgtgaagatt
    16141 cagaagataa agaagagaag cgttattata cgaactatta ctttaatcaa aatcattctt
    16201 tagagacagg ttggctttat gatcagtcta actggtatta tctagctaag acggaaatta
    16261 atggagaaaa ctaccttggt ggtgaaagac gtgcggggtg gataaacgat gattcgactt
    16321 ggtactattt agatccaaca actggcatta tgcgaacagg atggcaatat ctaggtaata
    16381 actggtacta cctccgttcc tcaggagcta tggcaactgg ttggtatcaa gatggttcaa
    16441 cttggtacta cctaaatgta ggtaatggag atatgaagac aggttggttc caggtcaatg
    16501 gcaactggta ctatgcttat agctcaggtg ctttggcagt gaatacgacc gtagatggct
    16561 attctgtcaa ctataatggc gaatgggttc ggtaatgaca gggcgcttat aattatatta
    16621 gaacactcgc ttaatcaaag aagtgacttt gttgtacttg acctgacgat tggggagatt
    16681 aaaagcatgt gaaatctaca catgcttttt cgttagttat gagtgttgaa cgtgactaac
    16741 cattgacaaa agataagagc aatcaaaata agatacaaaa atatcgatgc taactgcacc
    16801 gaaacgcccg atgctatgga tagggctaat agggagagca gggacggggc gagaatgtcc
    16861 actgtgctca gagcagagcc caccgtcccc atcttgtcac tggggatatg ggcaaagaca
    16921 gctgcttgta ggcgagggct gagaatgccg ataatcgtag aactgatgaa actggtcatg
    16981 aaaattacga tataggcttg acaaaggata ctagttatta gagtcaatag ggaaatctca
    17041 cagaaaacca agaggtgcgt gaaacggata tttttaaata gattactgct ggtcatattg
    17101 ccaaggatag ctccacaaga catgatgatg gagagcaggg cgaagagttg cccaatgctc
    17161 agttgtgcta gtgtctgttg gatgaagcgg agggcaatca gccgagggga cacatccaag
    17221 atggcaatca tactggtcgt cagaaacagt agtatgacgg tatcttttaa cctcaggagc
    17281 acagccagcg actgtttaag atgttgaaaa tatttcttaa aactcagtgc tgtatgtgac
    17341 atttcaatcc ttttttcaac ctcatacaag gtatgtcgaa taacatacag gcccaaaaag
    17401 gcaatgacaa aagtcagagt gttgataagg gaaatagttt gaatacttat aacattgata
    17461 aggaatgcgc cagccagatt ggcgacaatg cggacaaccc tcatcagaga ctgcctgaac
    17521 cccatggcat catgcaggtc gtccttaatt acactgatgt agagggcgtt catcatgtag
    17581 gcagacaggt agctcaaggt gtcagagatg aggttgatga tgacaatcat aatgactgaa
    17641 actaatgaaa agggcgacaa gacgagaaat actgtcaaaa tagcgtagag caggattttg
    17701 attttggcaa tacagagtgc gtgtttgact ctattttctt gaaaatctcc tagaatcccg
    17761 agaaagagtt gcgatagtag gggaaccatc tctgaaatgg aaatcagtaa aatcgctagc
    17821 ggagcgaaat tgtactgagc cacataattg ataatggcga ggtagaaaat actgtcgccg
    17881 aatgatgaaa aacagctgtt tagcagcaac tgtctatata tgttgtttgt ccaaaataat
    17941 ttcatacatt tctccttatg tctgcacatc gaatgggaat ttattattta atagacacca
    18001 atatacccca tgcttcctat tccgaagtca aacaaatctg ctttaccact atctccttga
    18061 aacatcagta aaccgtgatc atctcgttca gaacatgcta aaattacctg ttggattttt
    18121 ttcatcagta atttgttttg attgtagaca gtggtttgta gtaagctagt tattccgtgg
    18181 caatatcctg tcatcatttt tagatgatgg ttaaaaacaa attcttgata tttactaata
    18241 atatctttgt ttccgtcaca atcgtacata caaagatata agattattcc ggaaattcct
    18301 tcgcaccaag aaagttataa atttcctaaa ttttcagtat tagaagtaac tttttctaat
    18361 aattttttta at
//
LOCUS       DAVKJD010000032        18169 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKJD010000032 DAVKJD010000000
VERSION     DAVKJD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18169
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.032"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            362..1408
                     /gene="pheS"
                     /locus_tag="VW157_001152"
     CDS             362..1408
                     /gene="pheS"
                     /locus_tag="VW157_001152"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9552459.1"
                     /translation="MSTIEEQLKALREKTLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1408..1917
                     /locus_tag="VW157_001153"
     CDS             1408..1917
                     /locus_tag="VW157_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9552460.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1994..4399
                     /locus_tag="VW157_001154"
     CDS             1994..4399
                     /locus_tag="VW157_001154"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9552461.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEARQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKKFTLTETNEAAADALSVSIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVRAARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VRVPRRRWDITIEADLFEEIARIYGYERLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4467..5468)
                     /locus_tag="VW157_001155"
     CDS             complement(4467..5468)
                     /locus_tag="VW157_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9552462.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5624..6112
                     /locus_tag="VW157_001156"
                     /pseudo
     CDS             5624..6112
                     /locus_tag="VW157_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6197..6463
                     /locus_tag="VW157_001157"
     CDS             6197..6463
                     /locus_tag="VW157_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9552463.1"
                     /translation="MEGCLSELFSKEEILESDMRVAIMSELIEARNKQGISQKKLEEV
                     SGVSQPVIARMETGKTSPQLDTVLKVLASLGKTLAVVPLEQGKS"
     gene            6723..8972
                     /gene="metE"
                     /locus_tag="VW157_001158"
     CDS             6723..8972
                     /gene="metE"
                     /locus_tag="VW157_001158"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9552464.1"
                     /translation="MSTTIVGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATCIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGFDFVEGKKTLELVK
                     GGFPADKTLYAGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDTDYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9036..9902
                     /gene="metF"
                     /locus_tag="VW157_001159"
     CDS             9036..9902
                     /gene="metF"
                     /locus_tag="VW157_001159"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9552465.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQYMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10589..12802
                     /gene="pnp"
                     /locus_tag="VW157_001160"
     CDS             10589..12802
                     /gene="pnp"
                     /locus_tag="VW157_001160"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9552466.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12818..13435
                     /gene="cysE"
                     /locus_tag="VW157_001161"
     CDS             12818..13435
                     /gene="cysE"
                     /locus_tag="VW157_001161"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9552467.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13447..14331
                     /locus_tag="VW157_001162"
     CDS             13447..14331
                     /locus_tag="VW157_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9552468.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14413..15756
                     /locus_tag="VW157_001163"
     CDS             14413..15756
                     /locus_tag="VW157_001163"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9552469.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLDLGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMTKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15749..16135
                     /locus_tag="VW157_001164"
     CDS             15749..16135
                     /locus_tag="VW157_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9552470.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16139..17023
                     /locus_tag="VW157_001165"
     CDS             16139..17023
                     /locus_tag="VW157_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9552471.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGSQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            17160..17594
                     /locus_tag="VW157_001166"
                     /pseudo
     CDS             17160..17594
                     /locus_tag="VW157_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17902..18072
                     /locus_tag="VW157_001167"
                     /pseudo
     CDS             17902..18072
                     /locus_tag="VW157_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         5470 a         3492 c         4109 g         5098 t
ORIGIN      
        1 aattaatttt actttcccaa tcgatttgtt ttcatcttat ttcaatccgc tataaagtta
       61 gataagaata agctaacaag cagaagtaag ataggtcttg caagaatggt aaaaatctga
      121 taaactaagt agtaattgaa taggaatctg aaagactagt agcccaaggg aagtatatca
      181 gggaggagag ccgtgactgc aagctctcta tatgaaagct gggtgaattc acttgcgcat
      241 ggatttagaa atgaaggtct gacttgtcag atgaaaacgg atggtaccgc gtgtcaacgc
      301 tccgagtgga gtttttggca tgtggttttc tttttatcta cgagagactg atggaggaaa
      361 tatgtcaact attgaagaac aattaaaagc gcttcgcgaa aaaacgctga ctagcttgaa
      421 gcagattact gctggaaatg aaaaagagat gcaagatttg cgtgtctctg tccttggtaa
      481 aaagggttcg ctcactgaaa tcctcaaagg gatgaaagat gtttctgctg agatgcgtcc
      541 aatcatcggg aaacacgtca atgaagctcg tgatgtcttg actgctgctt ttgaagaaac
      601 agctaagctc ttggaagaaa agaaagtcgc ggctcaactg gctagcgaga gtatcgatgt
      661 gacgcttcca ggtcgtccag ttgcgactgg tcaccgtcac gttttgacac aaaccagtga
      721 agaaatcgaa gatatcttca tcggtatggg ttatcaagtc gtggatggtt ttgaagtgga
      781 gcaagactac tataactttg aacgtatgaa ccttccaaaa gaccacccag ctcgtgatat
      841 gcaggatact ttctatatca ctgaagaaat cttgctccgt acccacacgt ctccagttca
      901 ggcgcgtgct atggatgccc atgatttttc taaaggtcct ttgaagatga tctcgccagg
      961 gcgtgtcttc cgtcgcgata cggacgatgc gacccacagt caccaattcc accaaatcga
     1021 aggcttggta gttgggaaaa atatctctat ggctgatctt caaggaacgc ttcagttgat
     1081 tgtccaaaaa atgtttggtg aagagcgtca aattcgtttg cgtccatctt acttcccatt
     1141 cacagagcca tctgttgagg tggatgtttc ttgcttcaag tgtggtggag aaggctgtaa
     1201 tgtatgtaag aaaacaggtt ggatcgaaat tatgggggcc ggtatggttc acccacgtgt
     1261 ccttgaaatg agtggtatcg atgcgactgt atactctggc tttgcctttg gtcttggaca
     1321 agagcgtgta gctatgctcc gttatggaat caacgatatc cgtggattct accaaggaga
     1381 tgtccgcttc tcagaacagt ttaaataatg attagaaaag tagaaatggc agatgttgag
     1441 gtgttggcta aaattgccaa acaagccttt cgtgaaacct ttgcgtatga taatacggaa
     1501 gagcagttac aggaatactt tgaagaggct tatagtctga aaactttgtc aactgagttg
     1561 ggaaatcctg actctgaaac ctatttcatt atgcatgagg aggagatagc tggttttctc
     1621 aaagtcaact ggggaagtgc tcaaactgag agagaattag aggacgcttt tgaaattcaa
     1681 cgcctctatg tgctacaaaa attccaagga tttggactag gtaagcaact gtttgaattc
     1741 gcacttgaac ttgctacaaa aaatagtttt tcttgggctt ggttaggtgt ttgggagcat
     1801 aatacaaaag ctcaagcgtt ttataatcga tatggttttg aaaaatttag ccaacatcat
     1861 tttatggttg gtcaaaaagt agatacggat tggttactga gaaagaaatt aaggtaagaa
     1921 gaggattctt ctattgaaat gataggaaag gaaaagaact agagtgacaa ctgtcattct
     1981 ggagaactaa attatgcttg tatcttataa atggttaaaa gaattggtgg acattgatgt
     2041 gccatcacag gagttggctg aaaaaatgtc aaccacaggg atcgaggtcg aaggtgtcga
     2101 atcaccagct gccggtcttt caaaaattgt cgtcggtgag gtcttgtctt gcgaagatgt
     2161 gccagagact cacctccatg tttgtcaggt taacgttggc gaagaagaag cccgtcagat
     2221 cgtttgtggt gccccaaatg tgcgtgctgg gatcaaggtc atggtggctc ttccaggagc
     2281 tcgtatcgct gataactaca aaatcaaaaa aggaaaaatc cgtggtttgg agtcacttgg
     2341 aatgatctgt tcacttggtg aattgggaat ttctgactca gttgtgccta aggaattcgc
     2401 agatggcatc caaatcttgc cagaaaatgc cgtgccaggt gaggaagtct tttcttacct
     2461 agacttggat gatgaaatca tcgaactttc catcacacca aaccgtgcag atgccctttc
     2521 tatgtgtggg gttgcgcacg aagtagcagc catctatgac aaggcagtca actttaaaaa
     2581 atttactcta acagaaacta atgaagctgc ggcagatgcc ctttctgtca gcattgagac
     2641 agacaaggcg ccttactatg cagctcgtat cttggacaat gtgactatcg caccaagtcc
     2701 acaatggttg caaaaccttc tcatgaacga aggcatccgt cccatcaata acgttgtaga
     2761 cgtgacaaac tacatcctgc tctactttgg tcaacctatg catgcttttg acttggacac
     2821 atttgaaggg actgacatcc gtgtgcgtgc agcgcgtgct ggtgaaaaat tggtgacctt
     2881 ggacggtgaa gaacgtgact tggacgtgaa tgacctagtc atcactgtcg cagacaagcc
     2941 agtagccctt gcaggtgtca tgggtggtca agcaacagaa atctctgaaa aatctagtcg
     3001 tgttgtcctt gaagctgctg ttttcaatgg caaatctatc cgtaagacaa gtggtcgcct
     3061 gaaccttcgt tctgagtcat cttctcgctt tgaaaaagga attaatgtgg caacagttaa
     3121 tgaagccctt gatgcggcag ctagcctgat tgcggaactt gcaggtgcga cggtgcgtaa
     3181 gggcatcgtt tcagcgggtg agcttgatac ttcagatgta gaagtttctt caacccttgc
     3241 tgatgttaac cgtgtcctcg gaactgagct gtcttatgct gatgtagaag acgtcttccg
     3301 tcgtcttggc tttggtcttt ctggaaatgc agacagcttt acagtcagag tcccacgtcg
     3361 tcgttgggat atcacaatcg aagctgatct ctttgaagaa atcgctcgta tctatggata
     3421 tgagcgcttg ccaaccagcc ttccaaaaga cgatggtaca gctggtgaat tgactgtgac
     3481 acaaaaactc cgccgtcaag ttcgtaccat tgctgaagga gcaggtttga cagaaattat
     3541 cacctatgct ctgacaactc ctgaaaaagc agttgagttc acagctcaac caagtaatct
     3601 tactgaactc atgtggccaa tgactgtgga tcgttcagtc ctccgtcaaa atatgatttc
     3661 agggatcctt gataccgttg cctacaacgt ggctcgtaag aataaaaact tggcccttta
     3721 tgagattgga aaagtctttg aacaaacagg taatccaaaa gaagaacttc caaatgagat
     3781 caacagcttt gcctttgcct tgacaggctt ggttgctgaa aaagatttcc aaacagcagc
     3841 agttccagtt gatttcttct atgctaaggg aatccttgaa gccctattta ctcgtttggg
     3901 actccaagta acctatacag caacatctga aatcgctagc cttcatccag gtcgtacagc
     3961 cgtgatttca ctcggtgacc aagttcttgg tttccttggc caagtgcatc cagtcactgc
     4021 caaggcttac gatattccag aaacgtatgt agctgagctt aacctttcag ccatcgaagg
     4081 ggcgctccaa cctgctgttc catttgtgga aatcaccaaa ttcccagcag tcagccgtga
     4141 cgttgccctt ctcctcaagg cagaagtgac tcaccaagaa gttgtagatg ctatccaagc
     4201 tgccggcgtg aaacgtttga cagatatcaa actctttgac gtcttctcag gtgaaaaact
     4261 gggacttggt atgaagtcaa tggcttatag cttgactttc caaaatccag aagacagctt
     4321 aacggacgaa gaagtcgcac gctacatgga aaaaatccaa gcgtctctcg aagaaaaagt
     4381 caatgcagaa gtgcgttaaa cgatagtagg atacaaattc cccctctagt ttatgaacac
     4441 tagaggggtg attttatata aaataattaa aatgtgattt ctgtaaaaat acatctatga
     4501 tcagagtttt gaaaacttac aatgtctaaa tctttaacat agtagtggtt tttaggcaat
     4561 aaaatatgat ctattgttgc attaaaaagt tttggacttt ggctattcca agttcctctt
     4621 tcaaaaggtg gaagtgcatt caatgcgtct ctatgagagc ttatttttgc aagtgctcca
     4681 tgacgcatag ttgcattaaa atcacctgca ataatagcct ttggatattt tgaagccaat
     4741 tgattatgaa tgatgtttaa gtcttgcttc cagatttcca ttaaacctgg cagaggagga
     4801 gcagtatgca aggcaatgat atctggtata ttttgttttc tcgaatgtaa tacaattgtc
     4861 ccgaaccgtg ttgtatgaaa agttttagct tctgtataga aaccataact tttcttgaca
     4921 atcacagtca caggagctat tccactattg gtaggtggag aagtgaaaat atcatagttg
     4981 gccatagaaa gtccaacttg atgaaacaat agtttgattc tctggttttc ttgctcacct
     5041 ctgatattgg tagctagttc agggaatata gccatatcgg cgtcaaaatg gctaaaaatt
     5101 cgctcaatat gttgtgcttc tatttgatta gcgacgttcc aagttactaa ttttaaggtt
     5161 ttagtcttat ttgatgcaga agaaagggtt ttatctgttc cgaataatag taatatgagc
     5221 gatagtaaga gcaaaatgcc agagtaaaac cgtatttgtt tatttttctt gtaaaaagat
     5281 aggaaaaaaa ataagaaact taaaatagca gccatagttg cagtagctaa ggggaaggca
     5341 taaatctgtg ccaagatagg ttgggtagat agccaaatgt taagattaga agatgggaaa
     5401 aaagaataag ttatgatagg gatagaaaag agaatgaccg aaagggcaga gagaaatgtt
     5461 ttaatcattg tgtgagtacc tcaatttgca atggttgctc ggagctaatg tgactttgtg
     5521 actttacttg attgatattc tttttcttgt cttcctcata gattaagtac aacccaagta
     5581 aaaaaagtcc aatttaagat tgaaatgatt aaaaggcaca gaaatgcaag acaattatac
     5641 tacaaaaggc aaacatttga caatcgatag ccgtcgctta atcgaacgat ggaaaaaaga
     5701 aggaaaatca aatagagaaa ttgcctctct acttggaaaa gctcctcaaa ctatccatac
     5761 tgaaatcaag tatgggacag tccgaaaatg tcttggaaaa gggcgcttca aagaggttta
     5821 ttctgccgac tatgctcaac agtcttatga aaataatcgc aagcactcgg tcaagagatc
     5881 aagcttgacc aaggaactaa aggaaaagat tctccactat cataaccaaa aatttttgcc
     5941 tgaaatgatg gtgatggcta aaggggttaa cgtgggaatt tcaaccattt actattggat
     6001 tcatcatgga aagttggggt taagcaaaca ggatttgctt tatcctagaa aaggaaaatc
     6061 tgttaagaaa caagttagtc ccaattttaa acccgctgat caatctatcg aatcataatt
     6121 cgctcatttt ttcaaaaaat acatagatct aaaagaaaga ggggtaagtg atgaagaata
     6181 gtgctattgg gagtaattgg aaggatgtct atctgagctc tttagcaagg aggaaatcct
     6241 tgaaagtgat atgcgagtag ctatcatgag cgagttgatt gaagccagaa ataagcaagg
     6301 aatcagtcag aaaaagctag aggaagtcag tggagtgagt cagcctgtta tagctaggat
     6361 ggagacaggt aagactagtc cacagttgga cacagtctta aaagtcctag ctagtctagg
     6421 aaagacacta gcagtcgttc cacttgaaca ggggaaaagt tgactagaat cagttatagt
     6481 cttttctaat aacaagccat agtcacttat aagaattact aataacgcgt ttgagcattc
     6541 taactaaaat acagctaaaa acaagttata aaagggattt gagacgtttg cttcaagtct
     6601 ttttcttttg tccgaaacag atatatagtt tcaaactata tcaaacccta attttttaca
     6661 attatacaga cgctttcttt tctgttaaga tagtttcaac aacaaatttt ggaggacaca
     6721 tcatgtcaac tacaatcgtc ggtttccctc gtttgggcga attccgtgaa ttaaaattta
     6781 caactgaaaa atactttaga aaagaaatct cagaagaaga acttttagca gcagcaaaag
     6841 acttgcgtgc taaacactgg aacattgtca aagaaaaagg catcactgaa attccatcaa
     6901 atgacttttc tcactatgat aacttcctag atgcggcttt ccttttcaac gtggtgcctg
     6961 catccgttca aaacttggac ttgtctgacc ttgagcgcta cttcgctttg ggacgtggtt
     7021 accagggaga aaaaggggac gttcgcgccc tcccaatgaa gaaatggttt aacactaact
     7081 accactacat cgttcctaaa tttgaaaaag acactcaagt aaaattggct ggtcacaaga
     7141 tttttgatga gttccaagaa gccaaagaac ttggattgaa cactcgtcct gttcttgtag
     7201 gtcccttcac tttccttcaa ttgtcagact ttgaagaagg cgtgaaagca gacgacttcg
     7261 tagatagctt agtggctgct taccaagaag tttttgctaa attggctgaa cttggtgcga
     7321 cttgcatcca attggatgaa gcggctcttg taaaagactt gacagctgaa gaaaaagctc
     7381 tcttcttgaa tctttacaac aaactcttgg ctgacaaaaa aggtcttgaa gtcttgcttc
     7441 aaacttattt cggtgatgtt cgtgacgtat acgctgacct tgtaaacttg ccagtagatg
     7501 ctatcggttt tgactttgtt gaaggtaaga aaactcttga actcgttaaa ggtggcttcc
     7561 cagctgacaa gactctctat gcaggtattg tcaatggtaa aaacatctgg cgcaacaact
     7621 atgaaaagag cttggctgtt cttgagcaaa tcccagctga aaacatcgtt ttgacaagct
     7681 cttgctcact tcttcatgtg ccatttacaa ctgctaatga agaatttgaa ccagcactct
     7741 tgaaccactt tgcctttgca gttgaaaaat tggatgaaat ccgtgacttg gatgctatcc
     7801 gcaatggtca aggttcagaa gctcttgcag ccaacaaaga actctttgcg actgagcgtg
     7861 ttggtgaaaa tgctgaactt cgtgcgcgta tcgctggctt gacagacaca gactacactc
     7921 gtttgccagc ctttgcagaa cgtgaagcta tccaagaaga agctttcaaa cttccagctc
     7981 ttccaacaac aacgattggt tcattccctc aaacaaaaga agttcgtgcc aaacgtttgg
     8041 cttaccgtaa aggtgaattg tctcaaaaag agtacgatgc tttccttgct gaaacgattg
     8101 acgaatggat caagtggcaa gaagatattg actttgatgt ccttgttcac ggtgaatttg
     8161 agcgtaatga catggttgag tacttcggtc aaaacttgtc aggatacctc ttctctaaaa
     8221 atggatgggt acaatcatac ggtatgcgtg gggtaaaacc accaatcatc tggggtgatg
     8281 tcactcgtct taaccctatc actgttaaat ggtctagcta tgcacaaagt cgtacaaaca
     8341 aacctgttaa aggtatgttg actggacctg ttaccatcct taactggtca ttcccacgtg
     8401 aagacatctc tatcaaggat tcaactctcc aaatcgccct tgctatcaag gatgaagtac
     8461 ttgaccttga agctgctggt gtgaaaatca tccaaatcga cgaggctgct cttcgtgaaa
     8521 aattgccact ccgccgtagc gactggtacg aagactacct tgactgggct attcctgcct
     8581 tccgcttggt acactcaaca gtagcgccag acacacaaat ccacactcac atgtgttact
     8641 cagaatttac agatatcatc ccagctatcg ataacatgga tgcagacgtt atttcctttg
     8701 aagctagccg ttcaaacctt gaaatcttgg acgaactcaa agcgaaaaac ttccaaacag
     8761 aagtgggacc tggggtttac gatatccact cacctcgtgt accaaatgaa ggcgaaatcg
     8821 acaacacaat cgaagccatc cttgctaaag tgccaagcaa gaaagtttgg atcaaccctg
     8881 actgtggttt gaaaacacgt ggtatcccag aaacaaaaga aagcttgatc cgccttgttg
     8941 aagcagctaa agctgcgcgt gagaaattgt aagattggat ttctctccat acagtggttg
     9001 ttcaaaaaga aatcaactag aaaaggttat tacatatgtc acgccaaaca ccgtcactct
     9061 catttgaagt gttccctcca aacccagccg tgggtaatgg taacattatt tcagccttgc
     9121 agtatatgca ggagttggct ccccatttta tcagtgtaac tgccagcaat aataaattta
     9181 atatcaagga aacgacggtt cgtttggctg actttatcca aaatgatttg gcgattccga
     9241 ccattgccca cttgccagct atttatctaa ctaaggacaa ggttgctgaa actattgctg
     9301 acttggacaa agttggggtg cagaaaatct tggctcttcg tggggatatt attccagacg
     9361 tggaaccaca aaaggatttc cgctatgcaa ccgacttgat tgagtttatc aaggaacaaa
     9421 cccctcactt tgatattatt ggagcttgct atccagaagg gcatccagat tcgccaaatc
     9481 agatttcaga tattcaaaat cttaagaaga aagtggatgc aggctgttcg agcctcgtaa
     9541 ctcagctctt ctttgacaat gagcgcttct atgattttca ggacaagtgc atcttggctg
     9601 ggattgatgt tcccattcat gcaggaatta tgccaattct gaatcgaaat caggctctcc
     9661 gactcttgaa gacttgtgag aatatccatc ttccacgcaa atttaaagcc atcttagaca
     9721 agtatgagca tgaccctgag tcgctcagag cagcaggact tgcctatgca gtggaccaaa
     9781 tcgtggactt ggtaactcag gatgttgccg gtgtgcatct ctatacgatg aataatgctg
     9841 atacagcaaa atacatccat caagcaaccc atgccttgtt taatcaccag tctctaggat
     9901 aataaaagca aaccattctt ctcaggtgag gggaatggtt cctttttaat ggtaaagacc
     9961 tcacttttta agaaaaatat gataaaatag attctgtacg tacttgatac aaagatgaag
    10021 gtattaaata atacaatgca tttaaactgt aagatttaac gacacgagtg agtcaaatcg
    10081 atgttatttg acgagagagt tagtaaagca tttagctaat cgcccgatag cgattagcgt
    10141 gaaagattta gatactttag tattaaacct ttctaatgat ttatatcttg aaaactgaac
    10201 tcgcctacaa ctctgtggaa aaagataaat ctccctagga gcagtcgctc cgtcgttcga
    10261 tttcctattt tcttttgagt tgcttgacgg cttagtatct tgagtaattg aattttggtc
    10321 tcatttctta ggaaaaaaga taagcttcct agcactcgtc gagtgcgacg tcaccttcct
    10381 atttttctac agaaatgttc ggcaagccga accgtccaaa atatcttgtg taattgaaca
    10441 cggccgaaaa gctgtgtaaa aaagataaac tgtcttgtct tcatcgaaga cttcgtcagt
    10501 ttcctatttt tactttgttt ttgacgtcct tggtatcttg atctttgtag gcaaggcgta
    10561 taatttcatc aatccaaagg ggattaaaat ggcaaaacaa gtgtttcaaa cgacttttgc
    10621 gggtcgtgag ttaattgtag agactggtca ggttgctaag caagcaaatg gatctgttgt
    10681 cgtacgttac ggtgagtcaa ctgtcttgac tgctgccgtt atgtctaaga aaatggcaac
    10741 tggggatttc ttcccactcc aagtcaacta cgaagaaaaa atgtatgcgg ctgggaagtt
    10801 tcctggtggc tttatgaaac gtgaaggacg tccttcaaca gatgcgacct tgacagcgcg
    10861 tttgattgac cgtccgattc gtcctatgtt tgcggaaggt ttccgtaatg aagtccaagt
    10921 catcaataca gtgctttctt atgatgaaaa tgcatctgca ccaatggctg ctatgtttgg
    10981 ttcatctttg gcactgtcta tttcagatat tccatttgac ggaccaattg ctggggtaca
    11041 agtgggatat gtagatggcc aaatcatcat caacccaagt caagaacaag cagagcaatc
    11101 tcttcttgaa ttgacagtag ctggaaccaa gcacgctatc aacatggtag agtctggtgc
    11161 caaagaattg tcagaagaaa tcatgttgga agcgctcctt aaagggcacg aagctgtcaa
    11221 agaattgatt gccttccaag aagaaatcgt tgctgctgtc ggtaaagaaa aagcagaagt
    11281 ggaattgctt cacgtggatg ctgaattgca agctgaaatc attgcagcct acaacagtga
    11341 cctccaaaag gcagttcaag tagaagagaa attggcccgt gaagctgcga ctcaagtagt
    11401 gaaagaccaa gtgactgccg tttacgaaga aaaatatgcg gaccacgaag aatttgaccg
    11461 tattatgcgt gatgtggctg aaatcttgga acaaatggaa cacgcagaag tgcgacgttt
    11521 aattacagaa gacaaggtgc gtcctgatgg tcgtaaggtc gatgaaatcc gtcctttgga
    11581 tgcggttgtc gacttccttc ctcgtgtaca tggttcaggt ctctttactc gtgggcaaac
    11641 tcaagctctt tcagtcttga ccttggctcc gatgggagaa actcaaatca ttgatggttt
    11701 ggatccagag tacaagaaac gctttatgca ccactataac ttccctcaat attctgtagg
    11761 ggaaacaggt cgttacggtg cgccaggtcg tcgtgaaatc ggtcacggtg cccttggtga
    11821 gcgtgctctt gctcaagtct tgccaagctt ggaagaattc ccatacgcta tccgtctagt
    11881 agcagaagtt ttggaatcaa acggttcttc atctcaagct tctatctgtg cgggaactct
    11941 tgcccttatg gctggtggtg tgccaatcaa ggcgccagta gctggtattg ctatgggact
    12001 tatctcagat ggaaataact acacagtatt gacagatatc caaggtttgg aagatcactt
    12061 tggagatatg gacttcaagg ttgcaggtac tcgtgatggg attacagccc ttcaaatgga
    12121 tatcaagatt caagggatta ctgcagaaat cttgacggag gctcttgctc aagccaagaa
    12181 agcgcgtttt gaaatccttg atgtcattga agcaaccatt ccagaagttc gtctagaatt
    12241 ggctccaact gctccgaaaa ttgatacgat caagattgat gtggacaaga ttaagattgt
    12301 catcggtaag ggtggagaaa ccatcgacaa gattatcgct gaaacaggtg ttaagattga
    12361 tatagacgaa gaaggaaatg tgtctatcta ctctagtgac caagatgcta ttaaccgtgc
    12421 caaagaaatt attgctggtt tggttcgtga agccaaagtg gatgaagttt accgtgctaa
    12481 agtcgttcgt atcgagaaat ttggtgcctt tgttaacctc tttgataaga cagatgccct
    12541 tgttcatatc tctgagatgg cttggactcg taccaatcgt gtagaggatt tggtagaaat
    12601 cggggatgaa gttgatgtta aggttatcaa gattgatgaa aaaggccgta tcgatgcctc
    12661 tatgaaggct cttctacctc gtccgccaaa acctgagcat gatgaaaaag gtgaaaagtc
    12721 tgagcgccct caccgcccac gtcatcacaa ggatcacaaa cctaagaaag aatttacaga
    12781 aacaccaaaa gattcagagt aagaaaagga gaaatgtatg gggtggtggc gcgaaaccat
    12841 tgatatcgta aaagaaaatg atccagcggc ccgcaccact ttggaggttt tgctgactta
    12901 tccaggtgtc aaggccttgg cggcccaccg tctctcgcat tttctctgga agtacgactt
    12961 caaactctta gctcgtatgc acagtcagtt ttggcgcttt tggactcaga ttgagattca
    13021 tccaggagcc cagattgatt caggtgtctt tattgaccat ggttctggtc tggtgattgg
    13081 agagacagcg attgttgaaa aaggcgttct tctctatcac ggagtgactc tcgggggaac
    13141 agggaaagac tgtggcaaac gccatccgac tgtacgaaag ggagccctca tatcagccca
    13201 tgcccaagtt atcgggcctg tggaaattgg tgaaaatgcc aaggtcggtg ctgcagcagt
    13261 tgtcgtagca gatgtaccta gtgatgtgac ggttgtcggt attccggcca agattgtccg
    13321 tcttcatggt aagaaagatg agcctgttat tcacgaagtc gaagaaaaac gggagtatta
    13381 tgtcaataaa ctcgagcagg ctaaagatgc cagtcacaga tcgtctggtt tgtagaggat
    13441 acctatatga ttcaagcaag aaacaagtta agccaagaag agctatctga ggcgaaaaaa
    13501 gtaattaact gttgccaaaa ctatgacggt acctatcgcg atccctatct ctctaacatg
    13561 cttaattttg atccaaacat gcccgccttt ttcctttatt atgaaaaagg cgaacttgtt
    13621 ggtttattaa ctgtctatgc agatgaccaa gatgtagaag tgacgatact ggttcatcca
    13681 ggtcatcgcc gtcaggggat tgcgcgtgca ttgtttacta gttttgagag agaaacagct
    13741 tctttcccca ttcggtcagt cacttttcag acagaacgta tttttttaga gaatcatcct
    13801 gactttgcca gtaactgggg actaatagag gatgaagaga cagaaacttg gttaggtaag
    13861 gatagaagac cttatccgtt agcaaatgtt tcccatcttg aagttttgtt agcagatagt
    13921 tcgtatcagg atcaaattag tcaattaaaa tttcaggcat tttcagagga acatgaatcg
    13981 agagaagttg tggatagata tgtcgctgaa gctctgaagg atccagaaag tcgcttatat
    14041 attttgttaa aagacggtca ggttattggg acttgtacgg ttgatttatc gactaatacg
    14101 aattacttct acggtttagc aatattggaa cctgaacgtg gaaaaggcta tggaagctac
    14161 ttagcaaaat ccctcgtcaa ccaactaatt gagcaaaatg acaaggaatt tcagattgca
    14221 gtggaagata gcaatgtagg tgccaaacgt ttgtatgaaa aaattggctt tgtcaaacag
    14281 actcaggtgg tttatctgaa tgagaaagga gcaagggatt ccgaagtgta gagatattcg
    14341 gactgaaatt tacttgaact tttagtgatg aaactaattg ttcttggatt tcagctttcc
    14401 tgattatgat ttatgattaa aatctatgac accatgtctc gtgatttgcg agaatttgtc
    14461 ccgattgagg acggcaagat caagatgtat gtttgtgggc caacggtgta caactatatc
    14521 cacgtgggaa acgcccgttc gacggtagct tttgatacga ttcgtcgcta ttttgagtac
    14581 cgtgggtata aggttgccta tatttccaat tttacagatg tggatgataa gattatcaac
    14641 cgtgccaggg aagaaggcat cacgcctcag gaggttgcgg ataagtacat cgctgccttt
    14701 cgtgaggatg tgacggcctt aggcgtgaaa cctgcgactc gccatccgcg tgtagtggag
    14761 tttatggcag acatcatccg ttttgtggaa gacttgatcg agaaaggctt tgcctatgag
    14821 agtcaagggg atgtctattt ccgtgtagaa aaatcccaca actatgctaa attggctaat
    14881 aaaaccttgg aagatttgga tctaggtgct tcaggtcgta ccgatgaaga aacggctcgt
    14941 aaggaaaatc ctgtagattt tgctctatgg aaatcttcca aaccaggtga gatttcttgg
    15001 gatagtcctt ggggacctgg acgtccgggt tggcacattg agtgttcagt catgtcgaca
    15061 gagattttag gtgataccat tgatatccac ggtggtggag ctgacctaga atttccgcac
    15121 cacaccaatg aaattgccca atctgaagct aaaacaggca aggcttttgc taactactgg
    15181 atgcacaatg gctttgtcaa tatcgacaat gtcaaaatgt ctaagtcctt gggtaacttc
    15241 attacggtac acgatgccct taaaactctt gatgggcaag tacttcgttt cttctttgcg
    15301 acccagcact accgtaagcc tatcaacttc acggaaaagg cagttcgcga tgccgagacc
    15361 aatctcaagt atctgaagaa tacttacgaa caaccattta caggaaacgt ggatgcccaa
    15421 gagttacaaa actttaaaga taagtttgta gcagctatgg atgaagattt taatgctgcc
    15481 aacggtatca cagttgtctt tgaaatgacc aaatggatca actcagggaa ctatgatgca
    15541 agtgtcaagc aagctcttgc agatatgtta gaaatttttg gaattgtctt tgttgaggaa
    15601 gttttggatg cagagattga agacttgatt caaaaacgcc aagaggcgcg tgccaatcga
    15661 gactttgaga cagcagacca aatccgtgac caattggtta ctcaaggaat taagctcctt
    15721 gataccaagg atggagtgag gtggacacgt gattgatgtc aatctcatta acgggattgc
    15781 gctagccttt gagggggatg cggtgtattc tatgtatatt cgccgtcacc tcatcctcaa
    15841 aggtatgacc aaacccaata aactccatca agaagcaacc aagtatgtgt cagccaaggc
    15901 tcaggctcgc ctgattgctc tcatgttgga ggagcaggtc ctaacggaaa aagaagaaga
    15961 aatctacaaa cgtggccgca ataccaatag ccacacaaag gctaaaaatg cagatgtcgt
    16021 gacttatcgt atgtccacgg gatttgaagc agttatgggc tatctccata tgactgagaa
    16081 tctggaacgt cttgagagtt tggtttcatg gtgcatccaa aaagtggagg gctagaacat
    16141 gagggaaaaa gaactacaag actggtttcc tgaggctcgg atttcagacc aaccagtaga
    16201 gaaagagggc tatctcacgc tccctttagc ttctcagcag tggattttgc tggaggaagc
    16261 tgggctcagc gagcgtgaaa agcagttggt tgcccttttg acccagcagg agcaggctcg
    16321 ttcgctaaac ccttggtatt cctatctggt tgaaggcaag ggacaggcac cgcaagtttt
    16381 taaaaagatt cagttggttt attgccatct ttcttatttt cagcaggaaa atctggcttc
    16441 ttggctagat atgatgcgga ctctttttcc gaattgtcag acagtgctac aggtcggatc
    16501 tcaggattat gttttcgtgc ttcaacaaga caaatacact tctgtaagag atattttaag
    16561 tgatacgatt gaagcggttg agtatgactt tggacttcgt ctttctatca tgttgggtca
    16621 ggtttggtct cagacgggac atcaagccct atcagactta atcaaagctg agcgggattt
    16681 gttcaagaca tggtggcgtc agggtcacca aggtgttcat actttttctc agctctatct
    16741 ttggagtatg ggagaaagac tcgtggactt gaagccaatc aaggaatgtc tacaccagat
    16801 gattttggat caagatcaga ttcaggaaat cattctctct ctttgggaaa atagtgctgt
    16861 tctcactaaa acagcccagc aactctatct gcaccgcaat tctctccaat acaagattga
    16921 taaatgggaa gagttgacag ggcttcagtt gaaagagttg accgacctga ccttgtgtta
    16981 tcaattgatt ttaggttctt tgtcaactat agttggtttg taaagaagtt aatatttgga
    17041 gaagaggatg gccatcttct ccatttttat gtgcagagtt atagtggctt gatgctggga
    17101 aagtacactg tgactgctaa aacattccta gaagttgctt tgatttccct aatctattta
    17161 tgtgaatttt atgttatttt actataacag ttgttgctaa gccaaataat agtggggaag
    17221 tttatttaga cgtaagcatt gaagataatc agggaggtag tgggtataat ttcagttctg
    17281 tttcaagtag ctcacaaaca gctaaatatg aaggaactgg ttataataac aattcatcat
    17341 tatatataac gattgataaa acgtctgatg caacagctct tttgaaatta aagttgaata
    17401 atgttgataa tcaacctgct actgaagttc ctagttcagg aattactgta aaattaaatg
    17461 ctaaagataa tgctggaaac tggacaagtg cttcgaataa aaaagaaata acagtaaaaa
    17521 ttgtttctgc taaaccgaca tatccagaca aaatcttagt gaaaaatcct gataatataa
    17581 aagatacaga aaaaaatgcc attattgaaa aattgaaaga ggcaaataaa aatcatccag
    17641 caggagctcc aacctttgct aaaggtgaag gagagcatgc aaatgatatt gtagcaactt
    17701 attcagatgg tacaacttat tatgtaccgt taaatgatgt gacaaaatat gcgaggtagt
    17761 ggctgtacca ctcacttatt cacctccccg tgatttgtag tagtgatagg ctttctcact
    17821 attattataa aacaaaataa agatcacaac actttttcat tctgtgttgt gccttgagtg
    17881 aaacgaaagg aatgaattat aaatatgaaa agtatagtca ctaacataga tgagcgcttg
    17941 cgtactcgcc tacgagtgat tatctggaag caatggaaga agaaatcgag acgattatgg
    18001 ggattgctta agttaggggt tcctaaatgg atagcagata aggtatctgg ctggggcgac
    18061 cactatcaat tatattaaaa tgaaataaga ataggacgaa tcgatcaggg aagtcaaatc
    18121 gatttctaac aatattttag aagtagaggt atattattct agtttcaat
//
LOCUS       DAVKJD010000033        17629 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKJD010000033 DAVKJD010000000
VERSION     DAVKJD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.033"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..100
                     /locus_tag="VW157_001168"
     CDS             <1..100
                     /locus_tag="VW157_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9552472.1"
                     /translation="QKGFSLLELTEETSMLSHLKKEVEIYYQAKER"
     gene            complement(152..739)
                     /locus_tag="VW157_001169"
     CDS             complement(152..739)
                     /locus_tag="VW157_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9552473.1"
                     /translation="MKSITKKIKTTLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVGQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            981..1652
                     /gene="sdaAB"
                     /locus_tag="VW157_001170"
     CDS             981..1652
                     /gene="sdaAB"
                     /locus_tag="VW157_001170"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9552474.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            1661..2533
                     /gene="sdaAA"
                     /locus_tag="VW157_001171"
     CDS             1661..2533
                     /gene="sdaAA"
                     /locus_tag="VW157_001171"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9552475.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            2555..3187
                     /locus_tag="VW157_001172"
     CDS             2555..3187
                     /locus_tag="VW157_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9552476.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQVIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(3447..5297)
                     /locus_tag="VW157_001173"
     CDS             complement(3447..5297)
                     /locus_tag="VW157_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9552477.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(5339..6496)
                     /locus_tag="VW157_001174"
     CDS             complement(5339..6496)
                     /locus_tag="VW157_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9552478.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            6554..7720
                     /locus_tag="VW157_001175"
     CDS             6554..7720
                     /locus_tag="VW157_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9552479.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            7859..8185
                     /locus_tag="VW157_001176"
     CDS             7859..8185
                     /locus_tag="VW157_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9552480.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            8172..8765
                     /locus_tag="VW157_001177"
     CDS             8172..8765
                     /locus_tag="VW157_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9552481.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKRKRGNQHA"
     gene            8758..9669
                     /locus_tag="VW157_001178"
     CDS             8758..9669
                     /locus_tag="VW157_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9552482.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPIY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            9732..10796
                     /locus_tag="VW157_001179"
     CDS             9732..10796
                     /locus_tag="VW157_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552483.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVPQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(10973..11833)
                     /locus_tag="VW157_001180"
     CDS             complement(10973..11833)
                     /locus_tag="VW157_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9552484.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILG
                     QDFKTLAQEK"
     gene            complement(11955..12941)
                     /locus_tag="VW157_001181"
     CDS             complement(11955..12941)
                     /locus_tag="VW157_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9552485.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(13213..14688)
                     /locus_tag="VW157_001182"
     CDS             complement(13213..14688)
                     /locus_tag="VW157_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9552486.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(14898..15821)
                     /locus_tag="VW157_001183"
     CDS             complement(14898..15821)
                     /locus_tag="VW157_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9552487.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(15835..16764)
                     /locus_tag="VW157_001184"
     CDS             complement(15835..16764)
                     /locus_tag="VW157_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9552488.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(17083..17304)
                     /locus_tag="VW157_001185"
     CDS             complement(17083..17304)
                     /locus_tag="VW157_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552489.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(17258..17518)
                     /locus_tag="VW157_001186"
     CDS             complement(17258..17518)
                     /locus_tag="VW157_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552490.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         5217 a         3808 c         3314 g         5290 t
ORIGIN      
        1 ccaaaaaggg ttctcccttc tggagctaac cgaggaaact tctatgctgt ctcatctcaa
       61 aaaagaagta gagatctatt accaagcgaa ggaaagataa aaaagccgag ggatttcctc
      121 gactctttac atattatttg ttcgttagtt cttaatacca gccattgtta agccagaagt
      181 ttttagcagc agtccatgaa ccgtaacgtc ctgcaacgta ggcatctgct acacgttctt
      241 ggttttcagc tgagtagtca ccgttcaggt atgaatctgt taattggtaa cgtccgatat
      301 aacgtccatt tgtagctgta tagctaccac ctgattcttt ttgagcgatc cattctttgg
      361 cttctgcttc agatccgctt acagttgaaa caactgtttc acttactact ggagtttctg
      421 ctactggagc tgaaacagtt tcatcttgag cggctggtgc cgcataagta gctggcgctg
      481 gtgttgcaac aggcgctaca gggccatcga taaccaactc ttgaccaaca taaatcaaat
      541 gaatgttatc aatgtggttg ttttctgcca atttttcaac tgttgtgttg tgagtttcag
      601 cgatttctga aagtgtatca ccttctttaa cagtgtaagt tgatgattct tgagcagata
      661 caaatgatgg agcaaatact gcaaacaagg cagctactcc tgcaagagtt gttttaatct
      721 ttttagttat tgatttcata ttcgaaaatt ctccttcttt ctatagttct atcataccct
      781 ttaaatatta ccgtttcttg acacttttat gtagaaatat tacaaaatta ttttttattt
      841 ccctaaataa ggtgtttttt gtcataaaag atactatttt tatgctattt gaggtattag
      901 aaaggttttc atccacaatt aaagccaagc ccctcattct ttgatataat agagataaga
      961 atttttataa ggggaaactg atgaaatcac ttcgttttca atctgtcttt gatatcatcg
     1021 gaccagttat gattggccca tctagtagcc ataccgctgg tgctgttcgt attgggaaga
     1081 ttgtctcttc catttttgat gatactccga cagaagttga attccaactt tttaactcat
     1141 ttgccaagac ctatcgtggt cacggaacag acctagccct tgttgcaggt attttaggca
     1201 tggatacaga tgatcctgaa attccaaaca gtctggagat tgcccacaag cgtggtatca
     1261 agattgtctg gaccattcag aaagacagca atgcccctca ccccaacacc actaaaatta
     1321 ccgtcaaaaa tgcccacaag accatcagcg tgactggtat ttctatcggt ggaggaaata
     1381 ttcaggtaac cgaactcaat ggttttgccg tctctctcaa tatgaataca ccgactatca
     1441 tcatcgttca tcaagatatt ccaggtatga ttgcccttgt tacagaggcg ctttcccgct
     1501 atggtatcaa tatcgcccag atgaatgtca ctcgtgaaaa agctggtgaa aaagccatta
     1561 tgattatcga agttgacagt cgcaactgtg atgaggctat cgaagaaatt cgaaaaatcc
     1621 ctcatctcca caatgtcaat ttctttaaat aggaggaagc atgttttatt ctatcaaaga
     1681 attggtcgag caagcagatc tggactttca aggaaatgtc gcagaactca tgattacaac
     1741 agagtttgaa ttgaccggtc gcgaacgtga agaagtcttc cttctcatgg aacgcaatct
     1801 ggaagttatg aaagcctctg tccaacttgg cctcaatgaa aataaatctc gtagtggcct
     1861 gacaggtgga gatgctgcca aattggatca ctacatcaaa aacgaaaaaa ctttatcaga
     1921 ttacacgatt ctctctgctg cccgaaatgc tatcgcagtc aatgaacaca atgctaaaat
     1981 gggcttggtc tgcgctactc ctaccgcagg aagtgccggt tgtctccctt ccgttctcac
     2041 tgctgctatt gaaaaattag acctcagcca cgagcaacaa ttggatttcc tctttgctgc
     2101 tggtgccttt ggactagtca tcgcaaacaa tgcctccatc tcaggtgctg agggtgggtg
     2161 tcaagctgaa gttggttcag cctctgctat gagtgctgcc gccttgactc tggctgcagg
     2221 tggaacacct tatcaggcca gtcaagctat tgcctttgtc attaaaaata tgctaggcct
     2281 catctgtgac cctgttgcag gtttggtcga agttccctgt gtcaaacgta atgccatggg
     2341 agctagcttt gctttcatcg cagcagacat ggccttggca ggtatcgaat ctaaaatccc
     2401 tgtggatgaa gtgatcgatg ccatgtacca agtaggagca agcatgccaa ctgcctttcg
     2461 tgaaacagct gaaggtggac tcgctaccac ccctactggt cgtcgcctcc aaaaagaaat
     2521 tttcggagaa taagcttatc aaataggaga aaccatgacc tctatcacag cgattttttt
     2581 cgatttggat ggaaccctcg ttgatagttc tatcgggatt cacaatgcct ttacctatac
     2641 ctttaaagag ctgggggtgc ctagccctga tgccaaaact attcgtggtt ttatgggacc
     2701 acctctcgaa agtagttttg cgacctgcct gtccaaagac caaatttctg aagccgtgca
     2761 gatatatcgt tcttactata aggcaaaagg catctatgaa gctcaactct ttcctcagat
     2821 tatagacttg cttgaggagt tatcgagcag ttatccactc tacatcacca cgacaaagaa
     2881 tacatctact gctcaagaca tggcaaaaaa cttggaaatc catcatttct ttgatggcat
     2941 ttatggttct agccctgaag caccccataa ggcagatgtc attcaccaag ccttgcagac
     3001 acatcaacta gcaccagaac aagtcatcat catcggagat accaagtttg atatgctggg
     3061 agctcgagaa acaggtattc agaaattggc cgtcacttgg ggatttggag agcaagcaga
     3121 tctactaaac tatcaacctg attatatcgc tcacaaaccc ttagaagttt tggcgtattt
     3181 tcaatagcat agactaggta acaactccat ttcagaagaa aatgaagtaa tactcaatga
     3241 aaatcaaaga gcaaactagg aaactagctg cgggctgctc taaacactgt tttaaggttg
     3301 tagatggaac tgacgaagtc agtaacatat acatacggca aggcgacgtt gacgtggttt
     3361 gaagagattt tcgaagagta taagtctcta cataataaaa cgcatattac caaggttgtt
     3421 aacttgataa tatgcgtttt tcttttttat tcaatgtggg ttgtttgatt agcaaaggcg
     3481 ataatagctt gcttcaactc atctccactg agagtgcgga actctccaat cttttgattg
     3541 atggattcta aaggcatgac attaacctta ccaacgaaaa tcttatccat aacttgattg
     3601 tcaacgagtt cggttgatac caagagttct tcgtagagtt tttcacctgg gcggattcca
     3661 acttcaacga ttggaatttc actttcagtg tggccactta gaagcaccat cttcttggcc
     3721 aagtcataaa tcttgactgg tttgcccata tcaaggataa agacttcccc atctttggca
     3781 taagcaccag catggataac cagacggcta gcttctggaa tggtcataaa gtaacgggtc
     3841 atgcggaagt ctgtcaccgt tacaggccca ccttcagcaa tctgacgttc aaagactgga
     3901 atgacactac cacggctacc aagaacattc ccaaaacgaa ctgcacagta ggttgattgg
     3961 ctacgttggt taaagccagt gacaatcaac tccgccacgc gcttggttgc tcccataaca
     4021 tttggtggat tgactgcctt atctgtcgaa atcataacca tcttagacac tttagcttca
     4081 tcaacagcct tagcaacatt gtaagttcca cggatattgt ttttgaaggc ttcttttgga
     4141 ttgcgctcca tcataggaac atgcttgtgg gctgccgcat gataaacaat agcaggtttg
     4201 tactgctcaa agacttgcaa caaacgatca tagtcttgaa tgtccgcaat cacaggtaca
     4261 taatcaatcc cttggaactt acgaatcaat tcatgataaa caaggtagat tgagttttcc
     4321 ccatgaccga gcaagacaat gcgttcagga ttgaagcgac taacttgacg acagatttca
     4381 gaaccgattg aacctccagc tcctgtgact aagatggtct tacctgtcag ttctgcaccc
     4441 agacgcgatt cgtcaagacg gatttcctga cgacccaaaa ggtccgtaat atcaattttt
     4501 tggaagccag tacctgcttg gtgaaggccc tgaacaacag tttcaacctt aggcatcttg
     4561 taacatttga cacccagctt attacacatc tgcaagatac gctcatattc tgacggatcc
     4621 agcgacggaa tcgcaacgat gacacgctcg atttgatggc gtttggctaa ttcaggcaga
     4681 ttgtcataag agcccaaaac aggaatacca ccaagttttt gacccttttt cttagaatcc
     4741 ttatccaaaa taccgaccag ttctaattca ctggttggat gttggtaact atccataaag
     4801 agagccccac catcaccggc accaatcaag aaggtccgac ggtgttctcc atcaccacta
     4861 ccttttttgc gtctggagta gattaactgc caagtaatcc gtggcaataa aatcaagaag
     4921 gtactcaaca agataaagag aatgataaaa cggatggaga agagtggcaa gaaggcataa
     4981 cagatactat atgacaagac actgctagca gtcacaccaa aaaagatttt catgaaatcc
     5041 gtaatcttgc tgtaacgact aatgctcgcg ttcaaccccc aaaaaccaat catcaattga
     5101 tagaacagga aggccaaact cgtatagata atgtagtcaa caggtgctgg attaatcagc
     5161 ccataaaata aaatataaga tacaatgatg gaaaccacca tactgaaaat atcaaatatt
     5221 ccccagaaaa cctgcttttg ttgtttattt aaaatttcca ccagatcaat cacataatct
     5281 gttagttttt tattcataga aatctactcc tccttaaaag agttttacta tattgttatt
     5341 atagcatatt tttatttatt tggcttattt tatccaaata aattattgtt ctcttgtact
     5401 ccttcttcca tttaaatcct ctaattaaac ctacaatcgt cccaaagcca taagcaaagt
     5461 gaatggaaaa taaaagaaag ggcatcacaa ttagaaatcc atttttatgt tttaataaag
     5521 tcagcaaagt gagtagtgac aaaagtagaa aataggcacc taatagtaaa gttatgaata
     5581 cgaatgtgat cggtaacaat gctagactaa acacaagact caaaacaaat aaacaaggaa
     5641 catagtgaaa taatgataaa cacttaaact gaacatgact tgtcaagcca atccacaaac
     5701 catttaaata cttttgatgc agcattttct tgaatgttgg tcgaatatac tgataagata
     5761 gaatacttgg gctatagcgg attttataac catattctcg aattctataa tgaatatcat
     5821 tatcttcagt tcggccaagt tgctcattta ctaaaccaac cttctggaaa acctctcgtt
     5881 tatacatccc atgaaaaata gaagaaacat atctatcctc agaactattt cgataattgg
     5941 caatgctact gccaaacata ttttcctcaa caagatgcaa ggtctctgcc cattttcctt
     6001 ttccttcgac aatcgtcggt ctaggccccc cacagacaaa ttcaccttgt tgaataatag
     6061 ccacattgtt cattacaaaa ctctcagtaa cttttgaatg agcatcaatt tttaaaataa
     6121 ggtcccctac agaatgttta actcccaggt taaaaccact agcttgattt ttcttaggat
     6181 tgttatacaa tctaattgag ttaaactctg tatcttcctt tataaattgc tgaatgatag
     6241 ctgtggtccc atctgtggac atagcattta taaatagaat ttcaatattc tctttaggat
     6301 aggtttgatt ttttaagtct tcaattagac caggcagata tttttcttca ttgtatgcac
     6361 tgattacaat ggatacgaga agactttcaa cattttgagt cactactact ctcctgaatt
     6421 tttccttcca ccattctatc ataattttca agactttccc atttttatct tgaaagccag
     6481 aaaccttact tgacattata gtgaaaaagc cttaaaataa gctgttatta aaatcagcta
     6541 gaagaggtat tatatgaaaa agcaatcact cttttttgtt ccaggtatta tcctgattgg
     6601 tgtttccttg cgaactcctt ttactgtttt acccattatt ttgggaaata tttcgcaagg
     6661 tctggaggta gaagttagtt cgcttggtgt cttgaccagc ctgcctctcc ttatgtttac
     6721 cctcttttca ccattttcta cccaactggc tcagaaaatc ggcttggagc atctcttcac
     6781 ctacagcctc ttcttcttga ccatcggctc acttattcga ctaatcaatc tgcccctgct
     6841 ctatctagga accttgatgg ttggggcaag tgtcgcagtc atcaatgtcc tgcttcctag
     6901 tcttatccaa gccaatcaac caaagaaaat tggttttctg accaccttat atgtaacgtc
     6961 tatggggatt gcaacggctc tggcttccta tctagctgtg cccattacac aagccagttc
     7021 ttggaaagga cttatccttc tcctcacgtt actctgtcta gcaacttttt tggtctggct
     7081 cccaaatcac cgctataatc atagactagc tccacaaacc aaacaaaaaa gtcaaataaa
     7141 ggtcatgcgt aataaacagg tttgggcaat tattatcttt tcaggttttc aatccttgat
     7201 cttttacacc gtcatgacct ggttacctac catgtctatc catgcaggtc tatccagtca
     7261 cgaagctggc ttgctgactt ctatcttatc tctgattagc attccttttt caatgaccat
     7321 cccaagcctg acaaccagtt tatctactcg caaccgtcag ctcatgctca ctctggtttc
     7381 actagctggt gtggtcggca tttccatgct ctttttccca atcaataatt tcatttactg
     7441 gcttgccatc catctcctca tcggaaccgc aaccagtgcc ctcttccctt atctcatggt
     7501 caacttttca ctcaagacaa gcgcccctga aaagacagcc caattgtccg gcctatctca
     7561 aacaggaggc tatatcctag cagcctttgg gccaaccctc tttggttaca gttttgacct
     7621 gttccactct tgggtaccat ctgtagctgc cctcttgctc atcgatatcc tgatgactgt
     7681 ggccctcttt acagtggaca gagcggataa gatcctttaa acttctagtt ttagctagaa
     7741 gttttttata tataaaaatt ttacacattt ctcttgacag ggctttcttt tagatgtaca
     7801 atgtatgtgt agaaaaatta tatataaaaa tattacacat tagaaaagga ggtttcccat
     7861 gtactttcca acatcctctg ccttgattga atttctcatc ttggccgtac tggagcaggg
     7921 tgattcttat ggttatgaga ttagccaaac cattaagctg atcgctaata tcaaagaatc
     7981 cacactctat cccattctca aaaaattgga aggcaatagc tttctgacaa cctattctag
     8041 agagttccaa ggtcgcatgc gcaaatacta ctccttgaca aacggtggta tagagcagct
     8101 cttgacccta aaagatgaat gggcactcta tacagacacc ataaatggca tcatagaagg
     8161 gagtatccgc catgacaaga actgaatacc tgactcagct agaactctat ctcaagaaac
     8221 tacctgaagc tgaccgtatc gaagccatgg actatttcag agagctcttt gacgatgctg
     8281 gagtcgaagg agaagaagaa ctcatcgcta gtttgggaac tcccaaagaa gcggcccacg
     8341 aagttctatc caatcttctc gataaaaaaa tcaatgaagc acccgctcaa aaaaataacc
     8401 gacaaatttt acatatcgcc ttgttagccc tccttgcagc acctatcggc attcctctgg
     8461 gaatcgccat cctcgtgacc ctgttcgcaa tccttgtagc cgctttgact gtcattctgg
     8521 ctttctttgc agtttccata ctgggtatca tcggcggatt cctattttta gttgaaagtt
     8581 tcactgtcct cgcccaagcc aaatcagcct ttatcttgat ttttggttct ggtttactgg
     8641 ctatcggtgc ttcttcgcta gttttactag gcatttccta tgtagctcgc ttcttcggtc
     8701 tactcattgt tcgcctggta caatttgttc ttaaaaaaag aaagagaggt aaccagcatg
     8761 cgtaaatgga caaaaggatt tctcatcttt ggtgtggtga ctaccgttat cggctttatc
     8821 cttctttttg taggtatcca atctgacggg attaaaagcc tactttccat gtccaaagaa
     8881 cctatctatg atagccgtac ggaaaagcta acctttggca aggaagtcga aaacctagaa
     8941 attactctcc accaacacgc gctcaccatc acagactctt tcgatgatca aatccacatt
     9001 tcttaccatc catctctttc tgctcaccat gatcttatca ccaatcagaa cgatagaact
     9061 ctgagtctca ctgataagaa actgtctgaa actccgtttc tctcttctgg aattggtggg
     9121 attcttcata tcgcaagtag ctactctagt cgttttgaag aagttattct ccagctacca
     9181 aaagggagaa ctctaaaagg gatcaacatc tcagccaatc gcggacaaac caccatcata
     9241 aatgctagcc ttgaaaatgc gaccctcaat acaaacagct atatcctccg aattgaagga
     9301 agtcgtatca aaaacagtaa actcacaacg cccaatatcg ttaatatctt tgatacagtt
     9361 cttacagata gtcagctaga gtcaacagag aatcacttcc acgctgaaaa tatccaagtc
     9421 catggcaagg ttgaactgac tgccaaagat tatctcagaa tcatcctaga ccagaaagaa
     9481 agccaacgaa ttaactggga catctcaagc aactatggtt ctatcttcca attcacaaga
     9541 gaaaagcctg aatcaagagg tacggaatta agcaaccctt acaaaactga aaaaaccgat
     9601 gtcaaggatc aactcattgc gagatctgat gataatattg atctaatatc cacaccaagc
     9661 agacgttgac aaaaacgctt acatctgcta ctatggaagc atatttatct aactaaaaag
     9721 gaggttctac catgaaacaa gaatggtttg aaagtaatga ttttgtaaaa acaacaagca
     9781 agaacaagcc tgaagagcaa gctcaagagg ttgcagacaa ggctgaagaa acgatagccg
     9841 atctcgatac accaattgaa aaaaatactc agttagagga ggaagtccct caagctgaag
     9901 tcgaattgga aagccagcaa gaagagaaaa ttgaagctcc tgaagatagt gaagcgagaa
     9961 cagaaataga agaaaagaag gcatctaatt ctactgaaga agagccagac ctttctaaag
    10021 aaacagaaaa agtcactata gctgaagaga gccaagaagc tcttcctcag caaaaagcaa
    10081 ccacgaaaga gccacttctt atcagtaaat ctttagaaag tccttatatc cccgaccaag
    10141 ctccaaaatc tagggataaa tggaaagagc aagtgcttga tttttggtct tggctagtgg
    10201 aagcgatcaa atctcctaca agtaagttgg aaacaagtat cacacacagt tacacagcct
    10261 ttctcttgct cattctgttt tctgcatctt cctttttctt tagtatctat cacatcaaac
    10321 atgcttacta tggacatata gcaagcatta acagtcgctt ccctgagcag ctagctcctt
    10381 taactctttt ttctatcatc tctatcctag tagcgacaac actcttcttc ttttcattcc
    10441 tcttgggtag tttcgttgtg agacgattta tccaccagga aaaggactgg acgctagaca
    10501 aggttctcca acaatatagt caactcttgg caattccaat cttcctcact gctattgcta
    10561 gtttctttgc tttctttgat agcctacgat ttacagccct cttgtgtgtg attagcattg
    10621 gaatcattct gcttgccagt ctccatatca ttacaagacc aagtcaagca agtgaaaccg
    10681 actccttcta tcaattattc ttgtctgtcc ttgtgaacgg agttattatc ctcctcttct
    10741 ttgtagctga agtggcactg attggagatt atcttcgtat cttggccttt ctttaaactt
    10801 catactcttc gaaaatctct tcaaaccacg tcagcttcgc cttaccgtac tcaagtacag
    10861 cctgcggcta gcttcctagt ttgctctttg attttcattg agtagcaatc ctgtcatcct
    10921 tggcaggatt ttttctatgt caaaaaagca agtcgattga cctgcttctg ctttactttt
    10981 cttgtgctaa tgttttaaaa tcttgtccta ggatgggttg tatttctatt tgattggcgt
    11041 ctagttggtg gtaaaaagat gggggtaata cctctaagaa ttttgcatag tccaagcgag
    11101 ctatggattc atagtcttct ggtttagagc cgtctcctgt gatttgaacc aagtcaatct
    11161 cccactggac ttccttatcc accaattgct caatataggc tgcaggaaca aacggttctc
    11221 tcggtaaaac tgtcttgact ccttgagcta gatggtaaac gccatgcact tttccttctc
    11281 catcctgata gaaggaaact ctcgcaaagt aggcatctgt ctcttgaacc gcacgcacac
    11341 gcgcttcaaa ctgagccaag ccatctgcct ctaaaaagct tttcaagttc tcacgactaa
    11401 agaaaatagg attaaaaatt ccttggcaaa tcgtaaaacg agctacaggg tctgctagat
    11461 aggcttgaat acttgcttgg aaataagctt caaaatcaaa accatctagc ccttcaatat
    11521 cagtccctgt ataagcaagc aaagcatcta aaaatgactt gataatctgc cagtctgtct
    11581 ttgttagtag gtcaggaaga tcgacacggt aagccttttg accatcagca taactgacct
    11641 taaagagaaa ttgtgattgc cccactatcg cacactcaat atactcgaga cgattaagag
    11701 gctgacggag atagactaca tcatagctat gtgactccaa accgtctagc aaggccaaga
    11761 tcgacttggc attcaaaatc tcttgttctc ctaaaatgct ctgtttattt ggaataaaaa
    11821 atgtttttgc cataactgga ctcctttgaa atcgtttaca tacagtatac cttatttttc
    11881 tgaaagatac agaaacaaac tttagatttt tgagtacaaa gcatagaaaa acagcccctg
    11941 aggactgttt gatcttatac agtagctgct tgatccaagc tttcaccgat agcggctagg
    12001 cgctcaatca cttcagcttg tgtcaattca ttttttgaaa catagcggtt acgtgggtga
    12061 acacggcact cgtgtgagca tccacgaagg tacttgtctt cattttcttc tgatgtcaag
    12121 atacgacggt tacagaatgg atttccacag ttgacataac gttcacatgg tgttccatca
    12181 aaccagtctt tccctacgat agttgggttg acatggttga catcaacggc aatacgctcg
    12241 tcaaagacgt acattttccc atcccaaagc tcaccttgaa cttctgggtc tttaccgtaa
    12301 gttgcgattc ctccgtgcaa ttggccgaca tctttgtaac cttcacgaac catccagcct
    12361 gagaatttct cacagcgaac gccacctgta cagtaaacca cgacacgctt gtccatgaat
    12421 ttttccttgt tatcacggac ccattgtggt aactcacgga agttgcgaat atctgggcga
    12481 atagccccac ggaaatgtcc taggtcatac tcataatcat tacgtgtgtc aaggacaacg
    12541 gtatctttat caagaagcgc ttctttgaac tcttttggag acaagtaagc acctgttgtt
    12601 tcaagtgggt tgatgtcatt gtcaaagtcg ttgtcttcca aaccaaggtg gacaatttct
    12661 ttcttgtagc gaacaaacat cttcttgaag gcttgttcac tttcttcgtc aatcttgaac
    12721 cagagttctt ccattcctgg aaggctgtga acgtagtcca tgtatttttg agttgtttca
    12781 tagtcacctg aaactgttcc gttaattccc tcgtcagcga ctaggatacg gcctttaaga
    12841 ccgattgatt tacagaaagc caagtggtct gcagcaaatt gctctgcatt ttcaattgga
    12901 gtataaaggt agtaaagtaa gacacgaata tcttttgcca taagatttgt tctcttttct
    12961 attcttaaat tttctaaatt ttttatttat ctacactagt atacctgctt gagaaaacga
    13021 atgcaattta tttgactgga aattgtagag ggttctcgaa atttcctgaa tggttaaaat
    13081 aaggacaaga gaaaacatgg atatctatat ccttgtgcca aaaaaaccac tgtctccccc
    13141 agaccaacct gaggaaagca gtgattctta ttttaggagt taggaatgaa tacacgaaat
    13201 caatttagct gattattttt tgtttttcaa gaattcatcg tattgttttt gcatttcgtt
    13261 caatactttt tcgtaggcac cttcagattt caatttttcc atcaattctg gaatcgcttt
    13321 atctgggtct acagtaccag tgttgatagc tgtatcaaat tgttgcattg tgttagcaat
    13381 agctgagatt tcagatttca cattgtcagt attgaagata aatccaagcg ctggagattc
    13441 tttagcttct gccaattctt tcttagaatt ttcgatttgt tggtctgtaa cgttttcgtt
    13501 gatgtaaagg atccagttgt taccagtgtt ccatccaccc atgtgagtgt ttcctttgta
    13561 gccatcaaga acgcgaacac ggttttcttt accttcaatt ttttcccagt tcttgccttc
    13621 tggaccgtaa acaagaccgt tcaagagttc tggattcgta ttcaagaggt tcaagatttc
    13681 cattgatttt tctttgttct tagagttgtt tgagatgaca aagttagcaa cttgtgttgt
    13741 ttggtttttc ttgatgaagt tagtaattgg tttgatttgg atatctttgt tggcaacacg
    13801 tgaaagcaag ctgttaccgt agtcagctgg tcctactgtt tcttcacgaa cgaaccaagt
    13861 atcttgttga aggtcaaagg aagtatcgct tgttgcgacg tcttttggaa tgtagccagc
    13921 ttcatagaat ttgtgaagag tcttcaagtg ttctttgaaa cgaggtactt cgtaacggtt
    13981 tacaacttta gtagtatcgc cttcaaggtc aataacgaat ggaagaccgt ttgctactgg
    14041 gtagtcaaag ttatcagatg ggatgaaaac tttaccaata gcaaatggta ctacgtctgg
    14101 agctttttct ttaatttgtt tcaagactgg ctcaagagtt tcgtaagaag taacacctga
    14161 aatatcgata ccatatttgg caaggagagt tccgttgaag gcaaagtttt gagatgatgc
    14221 aacgttggct gcaactggta cagcgtaaat tttaccgttt acagtattac ccttgatgta
    14281 agctgggtca agtgccttgt aaaggtcttt accttctttt ttgtacaatt ctgtcaagtc
    14341 agcataagca cctttttgag catttacaat atagttatct gcaaaggcga tatcatagtt
    14401 ttcaccagat gatgtgataa ctgacatttt cttaccatag tcaccccagc caaggtattg
    14461 gatatccaat ttagcaccaa ctttttcttc gatgattttg ttggcatttg ctaacaattc
    14521 atccaagtta tctggtttgt caccgatttg gtacattttg ataacaggtt tgtcacctga
    14581 atcagcagct tttttgctgt tacctgtcaa atttccacaa gcagcaagac ctgcagccaa
    14641 agcgactaca ctagcagatg caaaagcata ttttttccag tttttcatga taaaaactcc
    14701 tttttttatt tttaaactta taaacaatgt aatgatctta tactcaatga aaatcaaaga
    14761 gcaaactaga aaactagccg caggctgtac ttgagtacgg caaggcgacg ttgacgcggt
    14821 ttgaatttga ttttcgaaga gtattaactt cacacaaggg aagttgggaa ctgagaaatg
    14881 ttctttttca ataagcacta ttctttcaca ccaccgatag tcaaaccttt tacaaagtag
    14941 cgttggaaga atggatacaa aatcgcgatt ggaagggttg ctaccacaac catggccata
    15001 cgacctgttt ctttcggtag agcaactccc agttgaccag tcaagccgac cgctttggca
    15061 atgtagtcca tattttgttg gatttgcatg agcaaatatt gcaatggata caagttgtca
    15121 ctcttgatgt aaagaagggc gttgaaccag tcattccaga aaccaagagc tgtcaaaagc
    15181 gtgatggttg cgatacctgg tagtgacaat ggcaaacaga tttggaagaa aatccgggcc
    15241 tcactggcac catcgatacg agccgattcg agaatggctt ctggaatggt cttcttgaag
    15301 aaggaacgca tcaagatgat gttaaagggg gagagaagca ttggcacaat caaggcccaa
    15361 acagtgtcac caagctgaag tacacgggtc accatgatat aacctggtac caaaccagcg
    15421 ttgaacaaca tactgagaag gacgaagatg gtaaaaaatc tgcgatactt aaaggttgtc
    15481 cgtgaaatag cgtaggcata ggttgttgtg ataaagacat ttgtcaatgt cccaactacg
    15541 gttacaaaga cagagatgaa gagggcttgt aggattttat ccttaaactg tgccaaaaac
    15601 tcaaaaccgt ctaagccaaa ttgggatggg aagaagctat agccgtattg gaggaggctt
    15661 ttctcgtctg tcactgaaat aatgataacg aatacaaaag gtaggataca agagagggca
    15721 atcaaacccg aaatgatact gaagaagata tctgctttct tactgaagga gtgaatgccg
    15781 acattatcaa ttttttcttt tttaattttc ttttttgcca tattctcctc ctttctagaa
    15841 caaagctgag tttggatcga ctcgtcttgc aagcaagttt gataggataa ccagaatcaa
    15901 accaacaacg gattggtaaa gaccggctgc tgcagccata ccaatatctg ctgtctgagt
    15961 caaaccatta aagacatata cgtccaaaac gttggttaca ttgtaaagct gaccagcatt
    16021 gtgtgggatt tgatagaaga gaccgaagtc tgcgcggaag atatttccga ctgcaaggat
    16081 ggtcaataca gttacaagcg gagtcaactg aggaatggtt acgttgcgaa tacgttgcca
    16141 cttgctagct ccgtccactg tcgctgcttc gtagtaggtt ggatcaattc ccatgatcgt
    16201 cgcatagtac atgacactgc tatatccaaa gcctttccaa atacctagga aaagtaggag
    16261 atagggccag atgcccaggt cagcgtagaa attgacttct ttgagaccaa gactttccaa
    16321 tagatgattg aacacccctt tatcaatatt taggaaggca tctgtaaaga aactgatgat
    16381 aacccaagac aagaagtaag ggaacaacat agaagtttgg aaaatcttca ccattctctt
    16441 agaacggagc tcgctgagga taatggcaat ccctacagat acaactaaac ctagaaagat
    16501 aaagccaaga ttgtagagga cagtatttcg tgtgataata aaggcgtctc ttgaactaaa
    16561 taagaatctg aaattatcga gtccgaccca tttactattt atgatactat ctatgaaacc
    16621 attactggtc atgtggtagt ctttgaaggc aaccacgttc ccaaatactg gaatgtaaaa
    16681 gaatagaatc aaccagagtg cccctggcaa aaccatcaag agaaagatcc agttgtctct
    16741 caatgttttt gaaaactttt tcataatttc ctcccttttt attttgatat ccatctaaaa
    16801 attctttttt agacttttga taacgattac attattagta tactcctatt tgcaggttag
    16861 gttaaactcc taattataga aaaaactcca caaattatgt agcagattta aaactttatc
    16921 accactatca aacaaatgtc ctaaatcaat tgtttatttt atctctatta gcccagtgat
    16981 gacgtcactc tgttataagc atccaacaac ggggtatact gaaaaatctc cagactaggg
    17041 aactcagcga tagttcctaa tctggagatt tttaatatgt tattaggcgt ttgctttcaa
    17101 cttagcaata acctctttaa gattatcaat cacctctgct tcagtatgct cagagccttt
    17161 ttcatctgcc aagaacaaaa ctgctttttg aagttctttt tgagagtttt caaggacatc
    17221 cttatctact gtttcaaggt ttgagtcttt aagaagttta cttaattcct tggctaattt
    17281 cttgagtttg atttgcagac tcatcttctc ctgctgtttc tttgcccgct gtttgtcctc
    17341 catccttagt tgctgactgg ctttccttaa tggactctag ggaagcaatg gcatctttga
    17401 ctgtttgcaa gatatcacgt aaaccttgct ctgtcaaact atcatctgca aaagctttct
    17461 tagcctctgc caaaaccaga cgtgctgaat ctgtggtagg attcgataca cctgtcaatg
    17521 atctcaaaag attttctaag gtttgagtct gcttactaat actagactaa aatcaaaaag
    17581 cattatataa cagtgatatg aaatcaacta aagaagaaat ccaaaccat
//
LOCUS       DAVKJD010000034        17353 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKJD010000034 DAVKJD010000000
VERSION     DAVKJD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.034"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(109..762)
                     /gene="phoU"
                     /locus_tag="VW157_001187"
     CDS             complement(109..762)
                     /gene="phoU"
                     /locus_tag="VW157_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9552491.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(774..1532)
                     /gene="pstB"
                     /locus_tag="VW157_001188"
     CDS             complement(774..1532)
                     /gene="pstB"
                     /locus_tag="VW157_001188"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9552492.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(1545..2348)
                     /gene="pstB"
                     /locus_tag="VW157_001189"
     CDS             complement(1545..2348)
                     /gene="pstB"
                     /locus_tag="VW157_001189"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9552493.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(2359..3243)
                     /gene="pstA"
                     /locus_tag="VW157_001190"
     CDS             complement(2359..3243)
                     /gene="pstA"
                     /locus_tag="VW157_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9552494.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(3233..4150)
                     /gene="pstC"
                     /locus_tag="VW157_001191"
     CDS             complement(3233..4150)
                     /gene="pstC"
                     /locus_tag="VW157_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9552495.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(4150..5028)
                     /gene="pstS"
                     /locus_tag="VW157_001192"
     CDS             complement(4150..5028)
                     /gene="pstS"
                     /locus_tag="VW157_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9552496.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(5602..6906)
                     /locus_tag="VW157_001193"
     CDS             complement(5602..6906)
                     /locus_tag="VW157_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9552497.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHTTGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKIFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(6907..7680)
                     /locus_tag="VW157_001194"
     CDS             complement(6907..7680)
                     /locus_tag="VW157_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9552498.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(7670..7948)
                     /locus_tag="VW157_001195"
     CDS             complement(7670..7948)
                     /locus_tag="VW157_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9552499.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(7948..8349)
                     /locus_tag="VW157_001196"
     CDS             complement(7948..8349)
                     /locus_tag="VW157_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9552500.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(8559..9176)
                     /locus_tag="VW157_001197"
     CDS             complement(8559..9176)
                     /locus_tag="VW157_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9552501.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(9166..9939)
                     /locus_tag="VW157_001198"
     CDS             complement(9166..9939)
                     /locus_tag="VW157_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9552502.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(9950..10687)
                     /locus_tag="VW157_001199"
     CDS             complement(9950..10687)
                     /locus_tag="VW157_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9552503.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKEA"
     gene            complement(10692..11822)
                     /locus_tag="VW157_001200"
     CDS             complement(10692..11822)
                     /locus_tag="VW157_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9552504.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(11906..12295)
                     /locus_tag="VW157_001201"
     CDS             complement(11906..12295)
                     /locus_tag="VW157_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9552505.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(12311..12694)
                     /locus_tag="VW157_001202"
     CDS             complement(12311..12694)
                     /locus_tag="VW157_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9552506.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(12695..13483)
                     /locus_tag="VW157_001203"
     CDS             complement(12695..13483)
                     /locus_tag="VW157_001203"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9552507.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(13476..14411)
                     /locus_tag="VW157_001204"
     CDS             complement(13476..14411)
                     /locus_tag="VW157_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9552508.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPKLLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(14541..14717)
                     /locus_tag="VW157_001205"
     CDS             complement(14541..14717)
                     /locus_tag="VW157_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9552509.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(14857..15564)
                     /locus_tag="VW157_001206"
     CDS             complement(14857..15564)
                     /locus_tag="VW157_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9552510.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            15715..16743
                     /gene="queA"
                     /locus_tag="VW157_001207"
     CDS             15715..16743
                     /gene="queA"
                     /locus_tag="VW157_001207"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9552511.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
BASE COUNT         5166 a         4012 c         3224 g         4951 t
ORIGIN      
        1 gttacttgtc aagtgttttt taaattttta tctcaaaaat attttttcgt tcaaaaaaag
       61 gagccatcag ttgatttcaa gctccttttt atacagaatt aaactatttt atagttcgac
      121 aatcttacct gtttcaaagt agacaaccca ttcacagata tttttagcat agtcaccgat
      181 acgctccaag taggaaataa cttggaaata atcacgaccc gtaacaatgg cttctggatt
      241 tttcttaatc tcttcagtcg caaggtcacg gatagtttca aaatagtggt taatttgctc
      301 atccatggag gccacccggt atgcgtcgtc aacagaacca ttaagataaa gatcaagtgc
      361 tgcttcaaca acgcttttaa cttcacgtcc cattttttta atttcttcct ctacagctgg
      421 aatgcgctct tcccccttca tacggatggt tgcctgggca atggctacag cgtgatcccc
      481 catacgctcc acatctgata cagccttaag gacagtcaag actgtacgca aatcttgaga
      541 gactggttgt tggagtgcga tcatttcaaa tgatttcttt tccagtttca cttcgtattc
      601 atttacttct gcatcatctt cgatgacctc ttttgccagg tcacggtcat gcgtgacaaa
      661 agcacgtacc gtacgattga tttgtgagag cacttcttgt cccatagcgt agaactggtt
      721 atgtaatttc tctaaatctt cttcaaattg agatcgtaac atctttcatc tccttatcca
      781 aattttcctg taatatagtc ttccgtttcc ttgtgttggg gatcaaggaa catctgcttg
      841 gtatcattaa attcaatcaa atctccatct aggaaaaatc ctgtcttatc agagatacgt
      901 gaagcttgct gcatggaacg ggttaccaga agcatggtgt acttgtcttt tagaccatac
      961 aaggtttcct caattttacc agctgaaatc ggatccaaag ccgaagttgg ctcatccaag
     1021 aggatgattt taggactagt tgccaagaca cgggccacgc agacacgctg ctgttgacca
     1081 cctgacaatc caatagctga atcatataga cgatccttga cctcatccca gatagaggca
     1141 ccttgcaagg ctttttctac ggcttcatcc agaacctgct tatccttaat tccattgata
     1201 cgaagcccgt agacaacatt ctcatagata gtcataggga aaggattagg ttgttggaaa
     1261 accattccga tttccttacg taattcaacc gtatctgtac gcggactgta gatgttgtgg
     1321 ccattgtaca ccacggatcc agttgtggtc acctctggat tgagatctcc catgcggttg
     1381 agagacttga ggagggttga cttccctgat ccagatggac caatcaaggc tgtaatttcc
     1441 ttaggttgga aagataggga aacactattc aaagccttct ttttattata ataaacggac
     1501 aggtctgata cctgtaaaat cgcatctgtc atacggtttc ctttctaacc aaagtgacca
     1561 gatacatagt cattagtgga ctgtagcttg gcattttgga aaatagttgc agtcttgtca
     1621 tactcaatca aatcacccaa gtaaaagaag cctgtatagt cacttgcacg agcagcctgc
     1681 tgcatattat gcgttacaat gatgatggta aagtttttct tgagctcaaa catggtctct
     1741 tctagttgca tggtcgcaat cggatccaag gctgaggctg gctcatccat taagaggata
     1801 tctggcttaa cagagatggc acgagcgata cagagacgtt gttgctgacc acctgataag
     1861 gtcaaggctg acttgtggag atcgtcttta acctgatccc agagggcagc ctgacgaagg
     1921 gaggtttcta cgatttcatc taggacttgc ttatccttaa ctccagcacg ttcatgcgca
     1981 aaggtaatat tacggtaaat tgacttagca aatggattgg ggcgttgaaa aaccattcca
     2041 atgtgtttac gcatttcata aacgttgatt tctggacggt tgacatcaat tccacgatag
     2101 agaatctgcc cagttacttt agcaatatca atggtatcat tcatgcgatt gagactgcgt
     2161 aagtaggtag atttccccga tcccgacgga ccaatcaaag ctgtaatttt atttctttca
     2221 aattgcatat caatcccctt aatggattca tttttaccat agtaaacatg gacatcctta
     2281 gtagaaaggg ctactttttc ttcaggaaag gtaaggatat gcttctcatc ccagttatat
     2341 gttgacatgg cttctccttt aggcagcggt taatttcttg tgtagatagc ttccgaactt
     2401 acgagctcca aagttaaaaa tcaggataaa gatcaggagc acagcggcag aacctgctga
     2461 tacaatggtt ccatctggaa tagtgccttc actattgact ttccagatat ggacagccaa
     2521 ggtttctgct tgacggaaga tagagatggg gctagtcaca ctgaggatat tccagttaga
     2581 ccagtcaaga gctggcgccg attgccctgc tgtatagatc agagctgcag cttcgccaaa
     2641 gatacgacca gatgccaaaa cgacacccgt tacaatacct ggaagcgctt ctggaataac
     2701 aacatgaacc actgtctccc agcgagaaat cccaagagcc agaccagcct cacgttgggt
     2761 atggtgaacg tgtttcaaac tatcctctac attacgcgtc atctgaggca agttaaagac
     2821 tgtcaaggcc aaggcacctg aaatgattga aaatccatac tcaaactgga ctacaaagat
     2881 caagtaacca aagagaccca ccaccactga tggtaaagag gacaagattt caatacaagt
     2941 ccgcacaaag ttggtaacag gacctttttt agcatattca gccaagtaaa tcccagctcc
     3001 catagaaaga ggtacagaaa taatcaaggt aatgaccaat aggaaaaagg aattgtaaag
     3061 ctgaatgcca atcccaccac ctgcttgaaa agcagaagac cttccagtca agaaagacca
     3121 agagatatgg ggcaagcccc gaaccaagat atagagaatc aaggaagcca agattgtcac
     3181 aatgatgcta gcaatcgtat agaggacagc tgttgcaagt ttatctaatt tcttagcgcg
     3241 cataattttt ctttcctctt tctttcgtaa tcaatttaat cacactgtta aaaactaaac
     3301 tcatcaagag cagtaccaag gccagtgacc agagaacatt attatttaca gttcccatga
     3361 cagtgttccc aattcccata gttaatatag aagttaaagt tgcagctggt gtggtcaagg
     3421 aagttgggat aacagctgag tttccgacaa ccatctggat agctaaagcc tcaccaaagg
     3481 cacgcgccat cccaaagacc actgcagtga aaataccaga acgggccgcc ttcaagatca
     3541 cacgccagat agtctgccag cgagtggctc ccatagcgaa actggcttca cgataataac
     3601 gaggaaccgc acgcaagcta tccgttgtca taaaggttac ggtcggcaaa atcatgacaa
     3661 agaggacgga aatccctgac aaaatcccaa aaccagtccc accaaataca ctgcgaacaa
     3721 agggaacgac gacttgcaag ccaataaatc cgtacactac tgaaggaatc ccaaccagga
     3781 gttcaatagc tggttgcaaa atcttcgccc cttttggtga tacttcggtc ataaaaactg
     3841 ctgcaccaat agcaaagggt gttgcgataa gggctgagag aatggtaacg ataaaggaac
     3901 ccaaaatcat aggaagggca ccaaattctt tactagaagg attccaagtt cctcccaaaa
     3961 gaaagtcaaa gatattcaca ccattgacaa agaaggtcga caaacctttt tgcgctacga
     4021 aaaccaaaat catggccaca aggatgacta tcaaagaaag acaggcaaag gtcaaacctt
     4081 ttcctaattt ctccagacga gaattctttg atggaagcaa cattttctta gctaattctt
     4141 cttgattcat tattgtctcc cttccaacac tgtcacagtt ccggcagcat ctttttcaac
     4201 cttcatttcc ttaatcggaa tatacttcaa tcctttgaca atcccttctt gggtctcatc
     4261 cgagagaaca aaattgagaa attctgcagc taactcattg ggctgcccca atgtatacat
     4321 atgctcataa gaccacaagg gccaattatt gctacttata ttttctggac ttaagtcata
     4381 gccattcaac ttcatgcttt tgaccgaatc atctatatag gtaagagata aataagagat
     4441 agctcctgga ctttttgata cgattgattt taccgctcca tttgaatcct gctcctgact
     4501 ttgcatggca gactgacctt ccataatgac agtatcaaag gtagcacgag agccagagcc
     4561 ggctgcccga ttgataacag agatgggtaa gtccttacca ccaacctctt tccaattggt
     4621 tacctcacct atgaagattt gacgaagttg ctctgtcgtt aggttatcaa catcaacctc
     4681 cttattgaca atcagagcca agccagctac cgcgaccttg tggtcaacaa gagcagaagc
     4741 atcaattccg tctttttcct cagcaaatac atctgagttt cctatatcaa ctgccccaga
     4801 ctgaacctgg gacaagcctg taccagaacc tcccccttgg acattgaccg tttttccaac
     4861 atggatcgtg ccaaattcat ctgccgctac ttcaaccaag ggttgcaagg cagttgagcc
     4921 aacagccgtt atggattctc cacgatcaat ccagctagca cagcctacta aacaagccgt
     4981 cagccaaaaa gcgataagag acagagcaag cttttttctt tttttcactg tttttctcct
     5041 cgaaaataat tatgaatact gtgaattttt taagtagttc tttatgagtt gacgcatgaa
     5101 ttcttaccaa atttctgcgc aattgattat ttatataata taggctatat tactctttcc
     5161 taacctcctt ttttcatatg tggataaaat ctcttgtcta tcccttcccc cattgtcacc
     5221 cattatagtc atttcgtgtc tctttttccc ctttttaatg caagggaaat tactctcctt
     5281 agatgataat ccaaaagcta gaaaggtatc tcaaacctct ctactctccc aaactagttt
     5341 acaactaaaa ggaaaagatt ctattttatg agaaatctag tttacaagcg gtaagaacgc
     5401 taataactaa acttcttgta ctctttgaaa atctcttcaa accagtgttt tgagctatct
     5461 atggctagct tcctagtttg ctctttgatt ttcattgagt agtaaaacta catgtaatgg
     5521 caatcaagat atcaagaatc atcctactaa aaaaatccat actttcacta taacatagaa
     5581 taagatattt gactagcatt ttcatttgaa tctgaggcct tttggaaaat aatttttcaa
     5641 aacatttcca gtaacctttg caaagcccaa gccattgcct ttaaccaaaa cttggtacca
     5701 accatttggc agactttctg ccagctgaac gatttctcca gccgcatact tgacaaacgc
     5761 ttcttggcca atttcaaccg actgttcgac ctgactcggt ttcaaggcta aaccaagagc
     5821 gaaactgggc tcaaagcgtt tcttcttaaa agtgcccaga tgcagtccat tgcgagcaat
     5881 cttgagcttc cctaaatctg gcaaaagttc tggcaagaga taaagctggt ctccaaaagt
     5941 ctgcaagata cccggtagat tgaccttcaa atggttttgg gcaaattcct gccacaaggc
     6001 aacttgttca cggctgaggt tactcttact tgccttaaat ttaggagctg gattgttacc
     6061 cttaaactgt agatgggcaa caaactgacc ctctccctta aactgatgag ggtacatccg
     6121 agccgtttct ggcaggtcaa taccagctac cattccattg atatgctcta ctggcaacaa
     6181 gtcaaaatca tactcttcca gcaaccaatt gacaatctct tcgttttcct cgggtgccca
     6241 ggtacaggtc gaataaacca gacgaccacc ttcagctaac atggtcactg catcctccag
     6301 aatttctctt tgcaagctag cacattgact cggataatct aagctccaat agtccatagc
     6361 atcaggttgc ttacgaaaca ttccttcacc agagcaaggg gcatcaagaa cgattaagtc
     6421 aaaatagcct ttaaagatct tgaccaagcg gtcggcagat tcattggtca ccacgacatt
     6481 tgtcgctcca aaacgctcca tattttcaac caaaatctta gcccgtttgc ttgaaatttc
     6541 attggaaaca agtagcccct cccctgctag ataggctgcc agttgagttg atttgccccc
     6601 cggtgcagca gccaagtcca agaccttcat accaggactg ggttgggcta cttgagccac
     6661 catttgagca gcaggttctt gcgaataaac taaacctgta gtatgctcag gcgatttccc
     6721 tgaaaccttc ccatagtggc cccaaggggt ttgagtaatg gcatcagaaa aggaaagttg
     6781 ctcttctttt aagggattga cccgaaaggc cgaaaccgct tcctcctcaa aagaggcaag
     6841 aaaatctctt gcctcatctc ctagtatctc tttatatttt tcaacaaatc cttctggaaa
     6901 ttgcatttaa gttcttttcc tttcgtaaat ataggactga atttcctcct gcatctcaag
     6961 aggcaccatc atgaccggct gtctggtttg aaaatcagga gcttcaccaa aaagggtcac
     7021 aacccgatag cccagacttt cccctaaaat actagctgcg gcataatccc atggttgcag
     7081 ataagtgaga taggtcaaca aacgccctga caaaatcttg gcaaaactaa tggccgcact
     7141 tccatagaca cgaacaccaa gaaccgctcg gctcaaatca gccagccccc attcattggt
     7201 ttccagcata ccactattcc ctgcaatgag aaaatctcca agtggtttag ttttaaaagg
     7261 agctaggggc ctatcattta gacaaactgg aaattcccca ccaccgtggt aacaatcccc
     7321 tttgaccaca tcataaatca gaccaaactg tccctgacca ttttcaaaat aagccatcat
     7381 aacagcaaaa tcttcctgct gggctacaaa attattggta ccatcaatgg gatcaatgac
     7441 ccaaaccttg ccctcttgaa ccgaggctcg cagacaacct tcttcagcac aaatcttatc
     7501 ctcaggataa cgggacaaaa tctcaccaac caagagttcc tgaacttctt tgtccagtct
     7561 ggtcaccaaa tctgttggag aggacttggt ttcaacacgc aagtcttcct gcatatggtc
     7621 aagaatgtac tgacctgctt tcttaacaag ctctttagca aattcaaatt tactttccaa
     7681 gagaaatctt tccttcccct ttttctttgg cagcctgaac tgctcggtaa agtgaatagc
     7741 cactaaccgt ttcaaattct cgtcccaaac gtttctcttc gctcttgctt ggcacgaccg
     7801 ctttgaatcc cttataggag tccagtaact ttttagcctc tactttgcct tcataggcag
     7861 cttcaacatc attaaaaaaa gaaagcactg aagcaagttc ttcagtgctc cacgacaaat
     7921 ctagtgggta actatactgt ttgttcatta actaatacca gctctcattc ttgcttcttt
     7981 tagttcttgc ttacgataac tacgagggag aaaagcacga atctcatctt cattaaaacc
     8041 gatttgcata cgcttggcat caataataat tggacgacgc aaaagactag gatactgctc
     8101 aatcaaatga agcaattccg ataccgaaat actctctaca tcaatattca atttttgaaa
     8161 aatttttgaa cgagttgaaa tgatgtcatc agtaccattt tcggtcaagg aaaggatgtg
     8221 ttgcaattct tttcttgtta aaggactggt cataatattg tgttccacaa agggaacctt
     8281 atgtttttct aaccaggcct tagccttacg acatgatgta cagctcggtg atagaaatag
     8341 tgtaatcatg cttttctctt cttatctata ctttgctact tctattatac aaaaaaataa
     8401 agcgcttgac tagggatttt tagaaaaaaa gcctattttt tcaagaaaaa taggcttttt
     8461 gcgaacgatt gacacaattg gatttggtta attcactctt aacgatggtt ttaaacgata
     8521 tatattttta tatatgtaaa ttaaaaacat ctttcctttc acttcctacg acttttcaga
     8581 tacagatagc caaagaagtt ttcatagagg gcaaaaaaga ggaggaaggc atgaagaaag
     8641 aaggtctctg gcaaaatcat aataacagga tccttggctg gatcaaaaag ccaggtatca
     8701 tctcccacaa agagaatttg atggaaaaga gtaaagaatt ggtcaaaacc aatcaaaact
     8761 cccccaagtc caatcatcac aggtaagact actagagcca ggagactttt tcgataaaga
     8821 gacaaaaagt cctttttcac aatcctattg acaaagacat agaaacttgg cagtgtcact
     8881 agagctacta gctgaaccaa atgaaagaga ttcttgacca ctgcgaaatg gtgcagacca
     8941 gctgctgacg aacgaaaatc aggcatctgt aagacctgac taaaaggatt ggtcagataa
     9001 ttcatcaaga tatgaaaatt gtattgaatg gtttctggtt ttagatagac tcgattcgtt
     9061 aagtttagcc actgaatctc cataggatag aaaatccaag ccagataaat ggtcagaagg
     9121 attgagaggg agaggagaaa gagcatagag ccccaaaagg tcaatttagt tttcatcaaa
     9181 atcccactcc gcaaggctag aaaccacatg tgtcggtgcg attggcaggc cagctacttc
     9241 ttctgcctta gtaaaacctg tcgtcaccaa gagcgttgga atgccattgt caatcccagc
     9301 ccggatatca gtcaaataat tgtccccaac catgattaac tcttcacgtt ccaaacctaa
     9361 gtgctcaacc gccttgtcca taatgatggc attgggtttt ccgatataaa ccggcttcac
     9421 tcgtgtcgct acttcaagca gcgtaatcag tgagccagca cctggcaaaa gaccgcgttc
     9481 cgtcgggatg ttgaggtcag gattggttcc gataaaatgg gcaccctttt gaatagcaag
     9541 agttgctgtg gcaaattttt catagtcgac ttgccaatcc agaccaacta ccacgtaggc
     9601 agggttttcc ttgtcttcca cataaccagc cgccttgatg gcttccttga gtcctgcttc
     9661 tccgacgaca tagacggtct tttcaagccc caaatcattc atatagtcga tggttgctaa
     9721 agtcgctgtg tagacagtcg ataggggcgt atcgatatta aaattctgag ccaacatctc
     9781 cttaacactc tctggagtgc gggttgtatt gttggttaca aagagatagg gaatgtcccg
     9841 cttttgcaat tcatgaacaa aagtctctcc agcagggatt cggtctttcc ccttataaat
     9901 ggttccgtct aaatcaatta aatagccttt atatttcatc tatttctccc taagcctctt
     9961 ttatttcttg ccaagtaatg attgcttggg cattgataac cccatcactt gtaatttcat
    10021 gcttgctttc cagtccagtc cgttcaacag ccgatgtaat caccccacct ggtcgaactt
    10081 ccttgacata cttgaggttg attttcttgg gaatatagtg ggtcaaaaaa tccgctccca
    10141 tgacctcaaa aatccagtcc aagtatttac tgttattgac atgaccattc atatccaagt
    10201 cgtaaaaacg aacatggtaa tccttgctga tcggctcttc caaggattca tacttcggtc
    10261 cacggataag ttttttatca aaatcagact ggtaaggagc cacaatctca ggttcaacaa
    10321 catggacttt tcgactgtcg cggtccatga gaacaaaggt cgccatcatg tggatgagct
    10381 cctgccccgc ttcattataa atagtaaagc gacggtagca aaaaagtcga ttgtagctca
    10441 aggcttccgt ttcgatggtg atttcttccg caaaacgagg caaacgaacc acctcaatat
    10501 catattctgc gataatccag accagattat agtcttccaa aatggcctta tcactaactc
    10561 ccagttcaat cgattgcatc cctgaaactt gcagtgacag caaaatcaca tctggaagtt
    10621 tgatatgacc gttcatatca gccatatcaa aaggaatttt cattttcatt tgataagtta
    10681 agcccatgat cctactccaa aataaatcgt tctgctacag tatctcccaa aaagagacct
    10741 ctctttgtca tgcgaacgtg gtcaccctca atctgcatga ggccttgttg aaccaaatct
    10801 ctgacaattt ctccataaag tccagcaaaa gactgtccaa atttttcctc aaatcgcgcc
    10861 atggaaaccc cggatttctt gcggagtccc aaaaacattt cttcttccat ttgctccttt
    10921 tgactcaggt gatcttctgt aatacaagca ttgccttcct caaccgcact gagataatga
    10981 cgaatgggac catgattttt atagcgtact ccattgacat aaccagatgc ccctgcacca
    11041 ataccatagt attcagcatt gtcccagtac atgagattat gacgactttc aaagccgggt
    11101 ttggagaaat tagaaatctc ataatgctca aaacccgctc gctccagctc tgcaatgatg
    11161 tactcaaaca tctccgcttc tagttcctcc ttaggcagag gcaatttccc acgtcgcatc
    11221 cggttcataa agaccgtatg gttttctaaa atcaaactat acaaactcat gtggggaata
    11281 tccaatccaa tggctttagc cacattttcc tttacttgct ccatggtctg accaggcaga
    11341 gcataaatca aatcaatgga gatgttgtca aaaccagcca gtttcaggcg atcgatattt
    11401 tcataaatat ccttctccaa atgactgcgc ccaatctttt tcaacatctt atcatcaaag
    11461 gtctggacac ctagcgaaac acgattgaca gccgaatttt tcaaaacagc tatcttatcc
    11521 gcatccaaat cgcctggatt ggcttcaatg gtcaactctt ccaagacaga caaatccaag
    11581 tttttagtca agccattcag taacacctcc agttgcggag ccgacagggc tgtcggtgtt
    11641 ccaccaccga tataaagggt tgacaacttt tcaatatcat aagaacgaaa ctcttccagc
    11701 agatgctcta aatagctgtc gactggctga tttttgatga agacctttga aaaatcacaa
    11761 taataacaaa tctgggtaca aaatgggatg tgcacatagg ctgacgttgg ttttttctgc
    11821 atagtaatta ttataccaca aagactagat tccagataaa aatcaccatc cccagataca
    11881 tagtccgtcc ggagatggtg atggtttatt cttctgttat atcaatcaca atctcttctg
    11941 agtcatcaag agcttcggct ttttcttgcc attgttcctt gagattattt aattgatttt
    12001 ttgatgcttc tgtcgcttga aaagcatagg atttagcttg agcaagtata ctgtccacag
    12061 tgatttcacc tgactcaacc tgttcttttg ttttcagaac aaaatctgta gcctgctcct
    12121 taacttctgt cagtttttca cagacttgct ccttggcata ctccggatct tctctcaaat
    12181 catctagaaa atcttgagcc tgactgcaaa cttgtttgcc cttatcactt gttaaaaaca
    12241 aggcaagagc tgcacctgaa accgttccta aaaggattga ggataattta cccataagga
    12301 ttctcctttt ttattttttg aaaaatttac ttgcaagacg aagagctgac agacttgcac
    12361 cagtcttgag tgtttttgaa ccagctgatg aagctttctt gctcaagaca cgcgcatggt
    12421 cattgaggtc tgaaacagat agagataaat ctgcaacagc actgaagagt ggatcaatcg
    12481 tagccacctt gacattgata tcatctgcca agacattgac cttagccaac aactcattgg
    12541 tgtgatgcaa ggtcacatcc acatctgaag tcaaggtttt aatcgtcttt tctgtttcat
    12601 cgatgacacg accaagcttt tgtacagtaa tgatcagata gaccaaaaag acaatcaaag
    12661 ctagggcaac aagaatatat gcaacttcta acatttagtt ttcctcctct gtaatatagt
    12721 aaggggcctt ctttcgattt tgataaataa cgatcattat accgagaccg ataaggacaa
    12781 ctgacagcca ttgggacact cgaaggccga agaacatgag actatctgtt cgcatacctt
    12841 cgatgaccat acgaccgaaa ccataccaaa tcaagtaaaa ggccgtgata tgacctcgtc
    12901 tgagactctt ccatttccgt ctaaaaatca gaatcaaggc aaagccaagc agattccata
    12961 gagactcata aaggaaagtc ggttgacggt agctcccctc aatatacatc tggtcacgga
    13021 taaagccagg tagataatcc agattatcca ctgttgcacc ataagcttct tggttaaaga
    13081 aattacccca acgccccaaa ctttgagcaa tcataacgct aggcgccgca atatctagaa
    13141 aatcccaagt attgatgagt ttacggtcag caaagatata gagcacaaga gccccagtta
    13201 tcaaaccacc gtaaatggcc aaaccaccat tccaaatggc aaaaatctct cctaaattct
    13261 gactatagta atcaaatcgg aaaataacat agtagagacg agctcctaaa atagccaagg
    13321 gaaaggctac taagataaaa tctaaaatat cgtctggtat gatcttcttt ctaggtgctt
    13381 ctttcatggt caaataaacc gcaagaatca agcctgtcac aatacataag gcataccaac
    13441 gaatagctag gggtcctagt tgaatagcaa ttggatcaag cattttgcac ctcatttcga
    13501 gcgatgagac ttgtcagtcg ttcgtcgaac aaacgggtcg catcaaagcc catttccttg
    13561 gcacgataat tcatggcagc tgcctcaatc acaacagaga tattacgacc tgttttaact
    13621 ggaatacgaa tacgaggaat ggctacgcca gaaacttcaa gttcctctgc attgtttcca
    13681 agacgatcaa aggtcttatg cgtatcgtaa ttttccaaat agacagcaag ctgaacctgt
    13741 gaagaatcct tgacagcact cgcaccgtag agactcataa catcgataat accaacccca
    13801 cgaatttcaa tcaagtgttt caaaatttca gctggttcac cccagagagt aatctcatcc
    13861 ttggcaaaga tatcgacacg gtcatcggct accaaacggt gaccacgttt gacaagctca
    13921 agacctgtct cgctcttacc aattccacta tctccctgaa tcaagacgcc catcccataa
    13981 atatccatca agacaccgtg cacactggta cgttctgcca aacgagaatc cagatagcta
    14041 gataactctc cagataaacg actggtagct gcacggctgg ttaaaatagc aatcttacat
    14101 tctctagcag cctttaacat ctcctctgga accaccaaac cacgggcaac aatgactgct
    14161 ggtgtctcag gtagaaacat ttttttcaaa acttcataac ggctgttgga aggcatgctg
    14221 atcagataag accactcctt catccccaaa agttgaatcc gctctggtgt atagtagtca
    14281 aaatagcctg tcatttcaag accaggtcgc gtaatatccg ctgtattgat ttccttttca
    14341 agcaattttg gttcaccata gacaatgtct agtctaagct tttcaatcac ttcttttact
    14401 aaaaccgaca tcatttcctc cttcaaatga gttcttctct ctattatatc aaattgtagg
    14461 aggaagtttc agatttttgc aggctttgga gtatttattt aaaagaaaaa ggactagatt
    14521 tctctagcct ttcatttcaa ttagaatttt ttacgtttac gagctacttc tgatttacgt
    14581 ttacgtttta cagaaggttt ttcatagaat tcacgtttgc gtgtttcttg aagagtacca
    14641 gctttagtaa ccgcacgttt gaaacggcga agtgcgtcgt caagagattc attcttacgt
    14701 actactgttt tagacatttt tttcactccc ttcaagttca atatctattt cattctacac
    14761 gtaaaaggaa taattgtcaa gcaaaaatgc ggtttaattt tgattttttc ttccatttat
    14821 acaagagttg aaagcgttca atagaacatt gcttttttat ttttcgagta agctaagcgc
    14881 ttcagcatct gcgatgatgg ttacatcagg gtgattttgg aggctacttg caggtaggtt
    14941 ctcagtcact gggccagata ctgttccggc aatggcttct gctttcgact caccgtaagc
    15001 aaaaagaata atagacttgg catccaaaat gtttttaatc cccattgaaa tagcttgggt
    15061 tgggacgtct tcaatcttgg caaagaagcg tgcattggct tcgatagtag actggtcaag
    15121 ttctactaga tgcgtttgac tgtcaaatgg agtaccaggc tcattaaagc cgatatgccc
    15181 attgcgaccg attcccaaga tttggaggtc aactggatgg tcagccaaaa tttggttgta
    15241 gcgttcaact tcagcttcag cattgtcctt aacaccacga ggcaagaaac tttctttaaa
    15301 tggtttttga ttgaacaagt tttcttgcat gaagtaacga taagactgtg gattgtcccc
    15361 atcaaggccc acatactcat caaggttgac actggttaga tttgaaaaat caaggtcact
    15421 ctcaacaatt tccttgtaaa attcaagtgg gctacttcct gtcgcaagtc ctagagtttg
    15481 agctccatta gccaattttt ccttcaaaat ctcaaaagca acttttccac cttgaacttg
    15541 gttttcaact ttaataactt tcatcttttc atcctccatt tctgtctata tttattatat
    15601 ggtatagacc aatttttgtc aagtgaaaac gatttaattt ctaaaaaaag agcgtccaaa
    15661 cttgaaacgt gttataatgg ataggattag aacttagaaa gaatgaacaa atatatgaat
    15721 acagctgatt ttgatttcca cttgccagag gaattgattg cccaaacgcc ccttgaaaaa
    15781 cgtgatgcct ccaaactcct catcgtcaac cgtgagacag gagaaatgca agataaacat
    15841 ttccactcta ttattgatat gctggaacct ggtgatgcac ttgtcatgaa cgacacccga
    15901 gttctccctg cccgcctcta tggtcaaaaa gtggagacag gaggtcatgt ggaacttctc
    15961 ctccttaaga acactagtgg agacgagtgg gaagttctgg ctaaacctgc caaacgcctc
    16021 aaggtcggta ctcgtatcag ctttggtgat ggccgcctca gcgctgtcgt tacagaagaa
    16081 ttgacccacg ggggacgcat tgtccgcttt gaataccaag gaattttcct agaagtcttg
    16141 gaaagtctgg gagaaatgcc tctgccacct tatatccacg aaaaattaga tgaccgtgaa
    16201 cgttatcaaa ccgtctacgc caaggaaagc ggctctgctg cagcaccgac tgctggtctt
    16261 cacttcacca aagaactgct ggcagaaatc caagctaagg gtgttcatct agtctatctg
    16321 actctccatg tcggactcgg aacctttaga cctgtttctg tggataatct ggacgaacac
    16381 gaaatgcact cagagttcta tcaactttct gaggaagctg ctgccaccct tcgctctgtc
    16441 aaagaaaatg gtggtcgcgt catcgctgtc ggaaccactt ctatccgcac cttggaaact
    16501 attggttcca agtttgatgg gcaaatccaa gcagattctg gttggaccaa tatctttatc
    16561 aaacctgggt atgagtggaa ggtcgtggat gccttctcaa ccaacttcca tctgccaaaa
    16621 tcaactctgg tcatgttggt ttctgccttt gcaggccgtg aattagtctt agatgcctac
    16681 caccattcca tccaagaaca ctaccgcttc ttcagttttg gtgacgccat gtttatttat
    16741 tgagaaagaa tttctctaaa tcttctaata ccaataaatc gctaagatat tatttcaaag
    16801 aacatctaca attgaaactc tagctagctg tagaagacgc ctagtacatt gaaattaaaa
    16861 tgcttccccc tagcttcgaa aatattgcca tagattgcgt tgactctcca aattgattca
    16921 tctatatttt atttcagctt cctatatttt cttcgctgtt tgtaaatcaa aatgcaagac
    16981 acatgagtag caccatattt gttactctta tctgtcctct caagagacta ttatgagtta
    17041 tttcagaatc attcactact ttgaccctga ctctccttag tctcaaaatc aaagacttat
    17101 actcttcaaa aatctcttca aaccgcgtca acgtcacctt ggattatata tgtgactgac
    17161 ttcgtcagtt ctatctacaa cctcaaagca gtactttgag caacctgcga ctagttttct
    17221 agtttgctct ttgattttca ttgagtatta aacaaaaagt gaacaaatct gaattctaat
    17281 gtacagaaga ctaggcttgt tcactttttt atagtcgcta taagatgacc ttatctatag
    17341 ctttttatat ata
//
LOCUS       DAVKJD010000035        17192 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKJD010000035 DAVKJD010000000
VERSION     DAVKJD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17192
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.035"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..274
                     /locus_tag="VW157_001208"
     CDS             <1..274
                     /locus_tag="VW157_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552512.1"
                     /translation="DNHVLTLIFLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALD
                     VIVYLFLGYLLVIQKAGYLFSVLLLFSNFSVPFIKEKEFELFKNSK"
     gene            1018..1248
                     /locus_tag="VW157_001209"
     CDS             1018..1248
                     /locus_tag="VW157_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552513.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQAGVFILEISYCTMM"
     gene            1233..1511
                     /locus_tag="VW157_001210"
     CDS             1233..1511
                     /locus_tag="VW157_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552514.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKITSYCIVAKDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            1797..3581
                     /gene="pspA"
                     /locus_tag="VW157_001211"
     CDS             1797..3581
                     /gene="pspA"
                     /locus_tag="VW157_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEU9552515.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTVVRAEEAPVASQSKAEK
                     DYDAAMEKYKAAEEDLKKAEAAQRKYDEDQKKTEEKAKETEEASKRQQAANLKYQLKL
                     REYLKYIQEKNKEKIAKAEKEMNEAKQEEDKEKANLNKVLAKVIPSDRELEKTRQEAE
                     KAKKNIPELKKKVEEAKQKVDAEHAKEVAPQAKIAELENQVHRLEQDLKDINESDSED
                     YVKEGLRAPLQSELDTKKAKLLKLEELSGKIEELDAEIAELEVQLKDAEGNNNVEAYF
                     KEGLEKTTAEKKAELEKAEADLKKAVDEPETPAPAPAPAPAPAPTPEAPAPAPAPAPA
                     PKPAPAPKPAPAPKPAPAPAPAPKPEKPAEKPAPAPKPETPKTGWKQENGMWYFYNTD
                     GSMATGWLQNNGSWYYLNANGAMATGWAKVNGLWYYLNSNGAMATGWLQYNDSWYYLN
                     TNGAMATGWLEYNGSWYYLNANGAMATGWLEYNGSWYYLNSNGAMATGWLQYNGSWYY
                     LNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKANQWFKVSDK
                     WYYVNGLGALAVNTTVDGYTVNENGEWV"
     gene            3981..5102
                     /gene="mnmA"
                     /locus_tag="VW157_001212"
     CDS             3981..5102
                     /gene="mnmA"
                     /locus_tag="VW157_001212"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9552516.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTTLLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVVRDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLKKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            5243..5698
                     /locus_tag="VW157_001213"
     CDS             5243..5698
                     /locus_tag="VW157_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9552517.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNRKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKSQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            5708..7621
                     /gene="mnmG"
                     /locus_tag="VW157_001214"
     CDS             5708..7621
                     /gene="mnmG"
                     /locus_tag="VW157_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9552518.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYSAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            7810..7899
                     /locus_tag="VW157_001215"
                     /pseudo
     CDS             7810..7899
                     /locus_tag="VW157_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8012..9691)
                     /locus_tag="VW157_001216"
     CDS             complement(8012..9691)
                     /locus_tag="VW157_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9552519.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(9693..9926)
                     /locus_tag="VW157_001217"
     CDS             complement(9693..9926)
                     /locus_tag="VW157_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9552520.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGTFEITEF"
     gene            complement(10744..10896)
                     /gene="cibB"
                     /locus_tag="VW157_001218"
     CDS             complement(10744..10896)
                     /gene="cibB"
                     /locus_tag="VW157_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9552521.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVSIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(10899..11084)
                     /gene="cibA"
                     /locus_tag="VW157_001219"
     CDS             complement(10899..11084)
                     /gene="cibA"
                     /locus_tag="VW157_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9552522.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            11262..11945
                     /gene="tsaB"
                     /locus_tag="VW157_001220"
     CDS             11262..11945
                     /gene="tsaB"
                     /locus_tag="VW157_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9552523.1"
                     /translation="MKVLVFDTSSKALSLAILEDKQILAEMTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQEYLPRTNFKETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            11942..12379
                     /gene="rimI"
                     /locus_tag="VW157_001221"
     CDS             11942..12379
                     /gene="rimI"
                     /locus_tag="VW157_001221"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9552524.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            12369..13379
                     /gene="tsaD"
                     /locus_tag="VW157_001222"
     CDS             12369..13379
                     /gene="tsaD"
                     /locus_tag="VW157_001222"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9552525.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWNLNAKPSLAFDT
                     ME"
     gene            complement(13419..14725)
                     /locus_tag="VW157_001223"
                     /pseudo
     CDS             complement(13419..14725)
                     /locus_tag="VW157_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15001..15352)
                     /locus_tag="VW157_001224"
                     /pseudo
     CDS             complement(15001..15352)
                     /locus_tag="VW157_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15459..15752)
                     /locus_tag="VW157_001225"
                     /pseudo
     CDS             complement(15459..15752)
                     /locus_tag="VW157_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            15995..>17192
                     /locus_tag="VW157_001226"
     CDS             15995..>17192
                     /locus_tag="VW157_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9552526.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     LLKIQLIHCHGYSTNYFLYMLKKLDKNGFGKVKTVITCHGWVEYNLKKKFLTYFDFWT
                     YSMGDAFICVSETMKKRLESIIKNKKIVAINNGINVSNSDLDVVGVQDFKKEFCIPNN
                     KKIICYVGRLDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICHLKCRD
                     NFRLLGEIYPATIVYQISDLLYIPYDTEGIPMCVLESMSQGTPVLASNVGGLSEIIEH
                     RVDGFLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHFSVQKMFVETMRVYD
                     ELLEKSS"
BASE COUNT         5226 a         3125 c         3857 g         4984 t
ORIGIN      
        1 tgataatcat gtactgactc ttatcttttt gataaaaatt tgcgtagata ctacgataaa
       61 atttttattc atcagattat ttgacacaag tactttaatg aagaggagaa tattttttct
      121 tttcgcattg gatgttatag tttacttatt tttaggatat cttttagtga ttcaaaaagc
      181 aggctatttg ttttcggttt tacttctttt ttctaatttt tcagttccat ttatcaaaga
      241 aaaggaattc gaattattta aaaatagcaa gtaatagaaa cataggttaa atcttagaac
      301 ggcttaaaat cagatatgat aaagggaaat agtcttatct aggtaactaa gtgctacaaa
      361 cacagtcgag gatcacttgt taggattaca ttttaatcat gcttaataag aatggagaat
      421 tcctatgggg attgtattac tctgaagttt cgaatacctt agttctcgtg attttgagct
      481 attctagctt tagaatcctt cagaatgtaa aattttaagc agtcaaagac ttagaatagt
      541 ccgaaaacat ttattttata gcaattctaa gattagaatt gctcatatat atgtatttga
      601 gctgctcgat agttaggaca gctctattct gagaaatctg agctatcaat gcttcagaat
      661 agtatttcgc caatggtttt tattgaagag gtgattgttt tatgatagtc taaaataaaa
      721 ctgatttaat ggagtaaatg gagaatcgtt atatgaaaca tttttttgca ggaattggtg
      781 agataaaact tgttggctac ttattgtata tatgtgtagg actagcccct ctttttcata
      841 tatatgtcat aggttcggaa ctgagttttg tgaaaatagt gttatctatt ttgggagttg
      901 tatttgtaag tatgttaact attgctcgaa tttatcggac ttttctataa agaatagttt
      961 ggacttgaac tgtatctaaa aacagaggtt ggtcgattat cagcctcttt cttgtcgttg
     1021 aaattacttg attgtatttt ggattatcaa gaaagattca atggaaaaac atgtcaagta
     1081 tcgacgaatt ataagtattt agagattttc aaagtgaatt tttgcttgac tgatttacat
     1141 catttatttg gcttacacaa aatcacacga gattatgcta gtcaaacaaa acctgctatt
     1201 caagctggtg tttttatttt agaaatatcc tattgtacaa tgatgtaatc gaaaggatat
     1261 ctttatatga gtttatcggt gatattttct attctaaaat aacaagttat tgcatcgtag
     1321 ctaaggattt atctaaaaat actatgaaat tggacgtcat attttttgag gataaaaata
     1381 aaagatccgc agttttaggt ttacgaagag ataaaagcgg agtatttaaa ccagttactc
     1441 tacattttac aagcgctaag aaatatgcta aagttcgtaa aacagatgtg aaagaaatga
     1501 aatggctata aagattgtcc gcaggcttaa acttgcgttt tttgttgttt aacgaaatta
     1561 aaaaataagc tctctcatcg gaaatgtttt tataggaatg aaggaagatg atgctatgtt
     1621 taaaaagaga atcgaaaagg gcaaaagtag tgtctttata tttttggagt agaagcttat
     1681 gatatagaaa tttgtaataa aaatgtaata taaaacattt gacaaatatt tacgggggga
     1741 gtatacttaa tataagtata gtctaaaaat gactatcaga aaagaggtaa atttagatga
     1801 ataagaaaaa aatgatttta acaagtctag ccagcgtcgc tatcttaggg gctggttttg
     1861 ttgcgtctca gcctactgtt gtaagagcag aagaagctcc cgtagctagt cagtctaaag
     1921 ctgagaaaga ctatgatgca gcaatggaaa aatataaagc tgctgaggaa gacttaaaaa
     1981 aagcagaagc tgctcagagg aaatatgacg aggatcagaa gaaaactgag gagaaagcga
     2041 aagaaacaga agaagcttct aaaagacaac aagctgcaaa tctgaaatat caactaaagt
     2101 tgcgtgaata tcttaaatat atacaagaaa aaaataagga aaaaatagct aaagctgaga
     2161 aagaaatgaa cgaagctaag caagaggaag acaaagaaaa ggcaaacctt aataaagttc
     2221 tcgcaaaagt aattcctagc gatagagaat tagaaaaaac tagacaagaa gcagaaaagg
     2281 ctaaaaaaaa tataccagaa cttaagaaaa aagtagaaga agccaaacaa aaagtggatg
     2341 cagaacatgc taaagaagtt gctcctcaag ctaaaatcgc tgaattggaa aatcaagttc
     2401 ataggctaga acaagatctc aaagatatta atgagtctga ctcagaagat tatgttaaag
     2461 aaggtctccg tgctcctctt caatctgaat tggataccaa aaaagctaaa ctattgaaac
     2521 ttgaagagtt gagtggtaag attgaagagt tagacgctga aattgcagaa cttgaagttc
     2581 aacttaaaga tgctgaagga aacaataatg tagaagccta ctttaaagaa ggtttagaga
     2641 aaactactgc tgagaaaaaa gctgaattag aaaaagctga agctgacctt aagaaagcag
     2701 ttgatgagcc agaaactcca gctccggctc cagccccagc tccagctcca gctccaactc
     2761 cagaagcccc agctccagct ccagctccag ctccggctcc taaaccagct ccggctccta
     2821 aaccagctcc ggctcctaaa ccagctccag ctccagctcc ggctcctaaa ccagaaaagc
     2881 cagcagaaaa accagctcca gctcctaaac cagaaactcc aaaaacaggc tggaaacaag
     2941 aaaatggtat gtggtacttc tacaatacgg atggttcaat ggcaacaggc tggctacaaa
     3001 ataatggctc atggtactac ctcaacgcta atggtgctat ggcaacaggt tgggctaaag
     3061 tcaacggctt atggtattac ctcaacagca atggcgctat ggcgacagga tggctccaat
     3121 acaatgattc atggtactac ctcaatacta atggtgctat ggcgacaggt tggctagaat
     3181 acaatggttc atggtactac ctcaacgcta atggtgctat ggcgacaggt tggctagaat
     3241 acaatggctc atggtactac ctcaacagca atggcgctat ggcgacagga tggctccaat
     3301 acaatggttc atggtactat ctaaacgcta acggcgctat ggcaacaggt tgggctaaag
     3361 tcaacggttc atggtactac ctcaacgcta acggttcaat ggcaacaggt tgggtgaaag
     3421 atggagatac ctggtactat cttgaagcat caggtgctat gaaagcaaac caatggttca
     3481 aagtatcaga taaatggtac tatgtcaatg gcttaggtgc ccttgcagtc aacacaactg
     3541 tagacggata tactgtcaat gaaaacggtg aatgggttta agccgattaa attaaatcat
     3601 gttaagaaca tttgacattt taattttgaa acaaagataa ggttcgattg aatagattta
     3661 tgttcgtatt ctttaggtac ctgtcttatg atttcaggaa atgtcattaa aaaaacgact
     3721 ccttttctct aacttggaaa atagattaga gaaaatgggt tgttttattt attatagtta
     3781 tttgaatgaa gataagaaga aggtatactc acatcattca caaatctgta tattgactat
     3841 aagttttaaa aaacaatttt taagctcttc cttgtcttct ctaaccaagc gtgttataat
     3901 gaatactgct caagcgacct tcaatcgtga agcacacacg accttcaatc gtgaataaac
     3961 gaatagatgg gagacttacc atgagtgata actctaaaac acgtgttgtc gtggggatga
     4021 gtggtggtgt tgattcgtcg gtgacgactc ttcttctcaa ggagcagggc tacgatgtga
     4081 tcggtatctt catgaagaac tgggatgaca cagatgaaaa cggcgtctgt acggcgaccg
     4141 aagattacaa ggatgtggtt gcggtggcag accagattgg cattccctac tactctgtca
     4201 attttgaaaa agagtactgg gaccgcgttt ttgagtattt cctagcggaa taccgtgcgg
     4261 gacgcacgcc aaatccggac gttatgtgca acaaggaaat caagttcaag gcctttttgg
     4321 actatgctat gaccttgggg gcagactatg tagcgactgg gcattatgct cgagtggtgc
     4381 gtgatgagga tggcactgtt cacatgcttc gtggcgtgga caatggcaag gaccagacct
     4441 atttcctcag ccaactttcg caagaacaac ttcaaaaaac catgttccca ctaggacatt
     4501 tgaaaaagcc tgaagttcga aaactagcag aagaagcagg tctttcgact gctaagaaga
     4561 aagactcgac agggatttgc tttatcggag aaaagaactt taaaaacttt ctcagcaact
     4621 acctgccagc tcagcctggt cgtatgatga ctgtggatgg tcgtgatatg ggcgagcatg
     4681 ctggtcttat gtactataca atcggtcagc gtggcggact cggtatcggt gggcaacacg
     4741 gtggtgacaa tgccccttgg ttcgttgtcg gaaaagatct aagcaagaat attctctatg
     4801 taggccaagg tttctaccat gattcgctca tgtcaaccag cctagaggct agccaagtcc
     4861 actttactcg tgaaatgcca gaagagttta cgctagaatg tacggctaaa ttccgttacc
     4921 gtcagcctga ctctaaggtg accgtccatg tcaaaggaga caaggcagaa gttatctttg
     4981 cggaaccaca acgcgcgatt acaccaggac aggcagttgt cttttacgat ggcgaagagt
     5041 gtctaggtgg cggtttgatt gacaatgctt accgcgatgg acaagtttgt cagtacattt
     5101 agattgacaa attttctcaa tttgctacaa taataacagc aatagaaatg atggtcaaag
     5161 ctcatggatg ttgcaggctt ttttgtcctg cacttctttg tagttttgac tgtttttgtg
     5221 tcgtttaagg gaaaggacaa gaatgactca acaagacttt cggacaaaag tagacaatac
     5281 ggtttttggt gttcgggcga cagctttgat tcttcaaaat cgcaagcttc tagtcaccaa
     5341 agacaagggc aagtattaca ttatcggcgg tgcgattcaa gttaacgaaa aaacggaaga
     5401 cgcggtagtc cgtgaagtga aggaagaact gggtgttaaa tctcaagctg ggcagctagc
     5461 ttttgtggtt gaaaatcgtt ttgaagtgga cggcgtttcc tatcacaata tcgagtttca
     5521 ttatctggtg gatttgcttg aagatgcccc gttgaccatg caggaagatg agaaaaggca
     5581 gccctgtgag tggattgact tggataagct tgaggatatc cagctagttc cagccttttt
     5641 aaaaacggcc ctaccagatt gggaaggcca actaagacac attcatcttg aggaatagga
     5701 gagaaacatg acttatcatt ttactgaaga atacgatatt attgtaattg gtgcgggaca
     5761 cgctggggtt gaggcttcct tggccgctag ccgtatgggc tgtaaggtcc tgcttgcgac
     5821 catcaatatt gaaatgctgg ctttcatgcc ttgtaatccc tctatcggtg gttctgctaa
     5881 ggggattgtc gtacgtgaag tcgatgccct cggtggcgag atggccaaga ctattgacaa
     5941 gacttacatc cagatgaaga tgctcaacac aggaaagggt cctgccgttc gtgcccttcg
     6001 tgcgcaggct gataaggaac tttactctaa ggaaatgcgc aagacagttg aaaatcaaga
     6061 aaatctgacc cttcgtcaaa ccatgattga tgagattttg gtggaagatg gcaaggttgt
     6121 cggtgttcgt acagcgactc atcaagagta ttctgctaag gctgttattg tgacgacagg
     6181 gactgctctc cgtggggaaa ttatcatcgg agacctcaag tactcatcag gtcctaacca
     6241 cagcttggct tctattaacc tagctgataa tctcaaggaa ttaggactcg aaatcggtcg
     6301 tttcaagaca ggaacccctc cacgtgtcaa ggcttcttct atcaattacg acgtgacaga
     6361 aattcagcca ggagacgaag tgcctaatca cttctcatac acttcacgtg atgaggatta
     6421 tgtcaaagat caagtgccat gctggttgac ctacaccaat ggtaccagtc atgagattat
     6481 ccaaaacaac ctccaccgtg cgcctatgtt tacaggtgtg gtcaagggag tggggcctcg
     6541 ttactgtccg tcgattgaag acaagattgt gcgctttgcg gacaaggaac gccaccaact
     6601 cttccttgag ccagaagggc gcaatactga ggaagtctat gtgcaaggac tttcaaccag
     6661 tctgcctgag gatgtccagc gtgacttggt tcattccatc aaaggtttgg aaaatgcaga
     6721 gatgatgcgg acaggttatg ctattgagta tgatatggtc ttacctcatc agttgcgtgc
     6781 aactttggaa accaagaaaa tctcaggtct cttcactgct ggtcagacaa atggaacgtc
     6841 aggttacgaa gaggcagcag gccaagggat tatcgcgggt atcaatgcgg ctctgaaaat
     6901 ccaaggcaag cctgagttga ttttgaagcg cagtgatggt tatatagggg tgatgattga
     6961 tgacttggtg accaagggaa ccattgaacc ctaccgtctc ttgactagtc gtgctgaata
     7021 ccgtctcatt cttcgtcatg acaatgctga tatgcgcttg actgagatgg gacgcgagat
     7081 tggccttgtg gatgatgaac gctgggctcg ttttgaaatc aagaaaaatc aatttgataa
     7141 tgagatgaaa cgcctagaca gtatcaaact caagccagtc aaggaaacca atgctaaggt
     7201 tgaggaaatg ggcttcaagc cgttgacaga tgcggtgaca gccaaggaat tccttcgccg
     7261 tccagaagtt tcttaccaag atgtggtggc cttcatcgga ccagctgcag aagacttgga
     7321 tgacaagatt atcgaattga ttgaaacaga aatcaagtac gaaggctata tttccaaagc
     7381 catggatcag gttgccaaga tgaaacgtat ggaagaaaaa cgcattccag ccaatatcga
     7441 ctgggatgac atcgattcta ttgcgacaga agctcgtcag aagttcaaac tcatcaatcc
     7501 agaaaccatc ggccaagcca gccgtatttc gggagtaaac ccagcagata tttctatttt
     7561 gatggtgtat ctggaaggta aaaatcgtag tatttctaaa actcttcaaa aatcaaaatg
     7621 atacgtcgtc ggcttcttac gaatgagttc aaagcttggc tttgattcat ctccagcctc
     7681 ccatagttcc ccgaactatg ggagctaact caagttatgc attttcctag ccttctggtc
     7741 tgattttcat tgagttttaa tctcaaaaga aagcatagag aaaaacagct ccgaagacgg
     7801 ggctgttttg ttgatttgta ctcaatgaaa atcaaagagc aaactaggaa gctagccgta
     7861 ggttgctcaa aacactgctt tgaggttgta gatagaactg acgaagtcag ctcaaaacac
     7921 agttttgagg ttgtggctag aactgacgaa gtcagtaaca tatatacggc aaggcgaagc
     7981 tgacgtggtt tgaagagatt ttcgaagagt attattcttc atctggtgtg aggatcatcg
     8041 ggatgatgat cggttcacgt tcggtatttt catagaggaa ggggcgaata gcgttgacaa
     8101 tggcaccatt gacagattgc acgctagcat ccttattttt cagtgcgata cgaatggcat
     8161 tgaagaggat acgctggctt tggcgaatca agtcgccaga ctctctcatg tagacaaagc
     8221 ctcggctgag gatgtctgga ccagatagaa tcatctgcga tttgaagtca acagttgcga
     8281 ctgccagaac gacaccgtct tcagatagat cgcgacgatc tttgaggaca gctgcgccaa
     8341 tttcaccgat acgatttcca tcgacataga tatcttgggc gttgaaatga cctgcgatac
     8401 gagctgagtc agcagtaagg gcaagcacat cgccattgct catgataaag atattgtcct
     8461 tctcaacacc agtatccact gctagtccag cgtggacttt ttgcatgcgg tattcaccgt
     8521 ggacaggcat gaagtatttt ggcttaatca agcggagcat gagtttttgc tcttgctgac
     8581 caccgtgtcc agatgtatgg atattgttca ctttaccgtg gataacttcg acaccagctt
     8641 cagaaatgat gttaatcagc ttgttgacac tagtagtgtt tccagggatg ggactagaag
     8701 agaagataac ggtatcacct ggttgtaatt gtacttgacg gtgggttccg ttggcgatac
     8761 gagagagggc tgccataggc tcaccctgac tacctgtaca gaggataaga acttctcctg
     8821 caggataatc tttgatttca tttggctcga taaaggttcc cttaggagct ttgatgtagc
     8881 caagatcgat tccgttgaca atggcctttt ccatagaacg accaaagacc gcaatcttgc
     8941 gtccagtctt aacagcagct tctgttgcct gctggagacg gaagatattt gaggcaaagg
     9001 atgcaaagat gatacgtcct tcaatacctt ggataatctt cataatggac tgaccaacga
     9061 ctttttcaga gttggtaaag gttggtactt ccgcatttgt cgagtcagac aggagacaga
     9121 gcacgccttc ttcaccaagc gcagccatac gatgcaagtc cgcaggttct ccaactggag
     9181 taaagtcgaa cttaaagtca cccgtacaga cgattttccc ttgaggagta tgaatgacaa
     9241 tccccaaagg ctctggaata gagtgagtcg ttctaaagaa agttgccttg agatttttaa
     9301 aggtcaactc ggtgttgtgg ttgatttcgt aaagtttggc gttgcgcaag aggccgtgtt
     9361 cttcgagttt cccacggatc aaagccaagg caagcggtcc agcataaata gggacatttg
     9421 cttgcttgag taggaacgga atcccaccaa tgtggtcctc gtgtccgtgt gtgattaaaa
     9481 cagccttgac gcggtcgata ttgtccacga tgtaagagta gtcaggaatg acatagtcga
     9541 taccaagcaa gtcatcttct gggaatttaa tcccagcatc gacgatgata atctcgtctt
     9601 ggtattcaat tccgtaagtg tttttcccga tttctcctag accaccgatg gcaaaaacgc
     9661 cgacttcttc aggtttaaga gtgtaggcca tattagaact ccgtaatttc gaaggtgcca
     9721 gtttcttttt cgtaatcgag caatttgtca gacaagagtt cgatatattc gatattgtac
     9781 tctgggcgat tttcttcgac aagttggcga gcagtgatac ggccctcaag ttctgagctg
     9841 gcatcgatgt ctaggtaaag cgcgcgtgtt gtttcacggc gtgggctacg ttcttttgtt
     9901 tcttgataaa aaactttgta aatcatatag ttcctttcta tttacaggtc agcttattcc
     9961 aaacttttag cagatatttg cttgatttca ttccattttg tgtctagatt gaccttgatt
    10021 cgttacaatt atttcaatta taccacaaaa tgaaaaatta gtaaaagacg gaaacgagaa
    10081 agttagggga cgatttcttc ggtaagcgtt tgcaaaaata aagaaaatgt gttatgttga
    10141 taacggaagt gaagtttatt acttgtcata attccatata gattttaagt caaggaggtt
    10201 gccctatgta taacttattt gcttttgcta tcggagttcg tttgttagct tttatcgttt
    10261 ttgttgcttg cgtttatgga ggaaatcttc tatttgccaa actggaacga tgacaaacca
    10321 agttcttgtg gaaaatgacc tttgtgactc tctactcgct ttttctcctc ctcgtgacct
    10381 atgtcgtttg gtttgtattt tactttggat taaatgctta gatgccctgc ttatgctggg
    10441 gttttttgtt tgggaatgta gagatagtag atttacgaca cgaaaattcc accccatcta
    10501 tcaagatgag gtggtctttg catttttcta acatgacatg acaatggaat tttcaaagct
    10561 tattattttt atgggagtca ttgttctgct caaaaataga gttgaagcat aaaaataaaa
    10621 caatacaaac agataaatcg aatggtaaag ggatagtagt aatgttcaaa tcattgagaa
    10681 tgctgagcaa actgtaagag atgaagacaa taaaatttat ccttctaaaa atgtgtttct
    10741 tatctagcat gacttaccaa actttttacg aaggtctttt ccaatggtat aacctgccat
    10801 aaataaagcg atactaacac ctacaccaaa gccacccttg atttcttgca attccttatt
    10861 agaaatttca cgatagttgt tcaaatcttt catcatagtt aatctcctcc taaaaaataa
    10921 ccagctgcca aaccagaacc tataagtgct gttccacctg caatagcctt ccaagatact
    10981 gctactccaa agataaccaa gggagcgaga ccgccatcaa tatctgataa ttcattttca
    11041 gataaggata aaaattgatt gtcaagaatg tcaaaatttg tcattgaatt ttcctccttt
    11101 tttcttgttc acttttatat tcggaaaaga aaaggaaaag tgagcatttt tttaaattat
    11161 ttttagttaa gtgagggcac tcttgtctgg gggcaaagaa aattcccatg tgccagcttt
    11221 tgtagtataa tagtaagcag acaaaaagat aggaatgtgt tatgaaagta ttagtttttg
    11281 atacgtccag caaggctctt tctctcgcta ttttagagga caagcagatt cttgccgaga
    11341 tgacgattaa tattaagaaa aatcacagta ttaccctcat gcctgccatc gattttttga
    11401 tggcaagttt ggactggaca cccaaggatt tggaccgaat cgtggtggct gaagggccag
    11461 gtagctatac aggcttgcga attgcggtag caactgctaa gaccttagct cacaccctga
    11521 acatcgagtt ggttggtatg tcgagtctct tggctctggt accctaccaa caagaaggtt
    11581 tgtttgtccc cttgatggat gcgcgtcgca ataatgttta tgcaggattt tatgaaaatg
    11641 ccaaacctgt catggcagaa gcacatctgc cttttgagcg agtgattgag ttaatcaagg
    11701 gtgctagtca ggttaccttt gtcggagaag ttggcccctt tgttgagcag attcaagaat
    11761 acttgccaag gactaatttc aaagaaacct tgccaaatgc agctaatcta gctctatggg
    11821 cttgggacaa ggaagcagac tccttgcatg attttgtgcc gaactacctc aaacgagtcg
    11881 aggctgagga aaactggctc aagaaccata ccgagtctgg cgagtcttac attaaacgcc
    11941 tatgatagaa atcaagcgaa ttcaacaaca gcctgaccta gctcaagcca tctacgctgt
    12001 tatggcagct gtttacctag tcagtccttg gactctggag caaatccaag cagatctgtc
    12061 ccaagaccag acttggtatg cattggctta tgatggggca gaagtgattg gctttctagc
    12121 tgttcaggag aatctttttg aggcagaagt cctgcaaatc gctgtcaaag gagcttatca
    12181 gggtcaggga attgcgtcag ccttgtttgc tcaattgccg acagacaagg aaattttcct
    12241 cgaagtcaga cagtcaaatc aacgagcgca agcattttac aagaaagaaa agatggcagt
    12301 tatcgctgag cgaaaggcct actaccatga cccagtcgag gacgccatta tcatgaagag
    12361 agaaatagat gaaggataga tatattttag catttgagac atcctgtgat gagaccagtg
    12421 tcgccgtctt gaaaaacgac gatgagctct tgtccaatgt cattgctagt caaattgaga
    12481 gtcacaaacg ttttggtggc gtagtgcccg aagtagccag tcgtcaccat gtcgaggtca
    12541 ttacagcctg tatcgaggag gcattggcag aagcagggat taccgaagag gacgtgacag
    12601 ctgttgcggt tacctacgga ccaggcttgg tcggagcctt gctagttggt ttgtcagctg
    12661 ccaaggcctt tgcttgggct cacggacttc cactgattcc tgttaatcac atggctgggc
    12721 acctcatggc agctcagagt gtggagcctt tggagtttcc cttgctagct cttttagtca
    12781 gtggtgggca cacagagttg gtctatgttt ctgaggctgg cgattacaag attgttgggg
    12841 agacacgaga cgatgcagtt ggggaggctt atgacaaggt cggtcgtgtt atgggcttga
    12901 cctatcctgc aggtcgtgag attgacgagc tggctcatca ggggcaggat atttatgatt
    12961 tcccccgtgc catgattaag gaagataatc tggagttctc cttctcaggt ttgaaatctg
    13021 cctttatcaa tcttcatcac aatgccgagc aaaagggaga aagcctgtct acagaagatt
    13081 tgtgtgcttc cttccaagca gcagttatgg acattctcat ggcaaaaacc aagaaggctt
    13141 tggagaaata tcctgttaaa accctagttg tggcaggtgg tgtggcagcc aataaaggtc
    13201 ttagagaacg cctagcagcc gaaatcacag atgtcaaggt tatcatcccc cctctgcgac
    13261 tctgcggaga caatgcaggt atgattgcct atgccagcgt cagcgagtgg aacaaagaaa
    13321 acttcgcagg ctggaacctc aatgccaaac caagtcttgc ctttgatacc atggaataaa
    13381 gagtggctct ttgtcaagtg tagtgggtag gcgaaaagct acaatctgga gagtacgaaa
    13441 ttcgtactct cttttttgat gttcaaagtc atcaaaatcc gttttttaaa attgttaaag
    13501 ttccggaaac caaaggcttt gcgcttgata accttaatca aattattggt cgcttcaagt
    13561 ttagcgttgg aataaggcag cttaagtgcg ttgataatct tatctctatc ccttaaaaaa
    13621 gtccaaaaga ccgtttgaaa aagcggatga accgttggca gttcttgctc aatcaatcca
    13681 aagaaatggt cggcattctt ctcttgaaaa tgaaagagca ggagttgata gagttcgtag
    13741 tgaacctgta atccctcaga gtaagaaagc agtttatctc gtacttcccg atgggttaag
    13801 tgcattcgaa acgtgggctg atagaatcgt ttctgacaga gcttgaagct atcctggtgt
    13861 agtaaatcag tccagtggac tgatttagcc cgagcctaga aacgaaaaag cgagggttcc
    13921 aaaagcgctt gagcgcccga tactccaagg attttcggtc aaattggttc atgatttgga
    13981 ttcgtactcg gttcatagct cggctcagat gttggacaat gtggaagcgg tcaagaacaa
    14041 tcttcgcctt tggaaagagt tgcttagcga gccgataata agggctgtac atgtccatgg
    14101 tgatgacctc gaccagctcc cgaacctctc tctgatagcg ttggaaatgg tttcgaatca
    14161 ccgcatgaat tcgcccgtct aaaattgcga ggatggattt ggactcaaaa tcttgggcaa
    14221 tgaagctcat tttgctcttt ttgaagctat actcatccca actcataact tttggcaact
    14281 tggtccaatc ggtttcaaac ttaaattccc tcagttttcg gatgacggtg gaagttgaga
    14341 ccgccaatct gtgggcaatc tcagtcatag actgcttttc aaggaggagc tgagcgattt
    14401 tttgtctcac catattggaa atctggcaat ttttcttgac aatggatgtt tgagaaacga
    14461 ccaccttaag gcaattcttg cactgaaagc ggcgcttttt gagatgcagt accgtgggta
    14521 aaccctgaca gtcgagaagc ggaattttag aggctttctg gaagtcgtat ttgatcatct
    14581 tcccttgaca atgaggacaa ggaggagcgg gggaatccaa ctttgcctga acgactagat
    14641 gggtttggta tttcagaaca gacaagattt tgatattttt gtcttccaat ccgagcaaat
    14701 ctgtggtatt cttaagttgt tccatatgaa ccctttctaa tgagttggtt agacgctttt
    14761 cattataggt catatggggc tttttttcta caagaaacga ccctataatt cctggggtgg
    14821 gattacccac tacagaaatt atagagccaa agcattccaa agtcttgtct gactctggaa
    14881 tgttttctgt tcacagttgt tttgtaactg cctaataagt aaccgcgcta taacgaggta
    14941 tctaccgaga ttgtcaattc tccagtatac tgttataaaa aacaaaactg gaggattttt
    15001 ttatgtcccc ccatcgtccc atcgactgtt ccgtagctgc ccaataacgg agtatagtcg
    15061 gctgaaacaa gaataggaca aaagtgcctc aagaaaagca tcgtaatttg acaatattat
    15121 tctgagatgt ttttgatgta agcccatatt ttctcaatgg gattatactc gggtgaatag
    15181 gtaggaagtg gtaacagtct atgccctgct ccttacacat gttcattctg tgaaaccttg
    15241 cattgtccat aatgataagg gatggtgtat ctaaagtagg tagtaagaat ttttggaagc
    15301 aagcttcgaa aaagtcactc gtcatcgtat ctttataggt catcggagcc atagtatatt
    15361 gaaacaaaaa agggcattag tacttctata acagtatcgc aatctctggt gtgcgttgtc
    15421 tcgatcttaa ttcaaataat tatatcttct tccagagaga ctaactgcct gctcaaggct
    15481 cgatcatatt cgagataaaa ataggtctca acccctgtct cataaatata aataggagtc
    15541 aggtagctta agtgattcaa ttctttaagg aaccgattta ctttttctgg gtcttgttcg
    15601 cagtaggtac ggctcttttt tagactatat cccatagcct ttagagcgta atgaatagtt
    15661 gttggaggac agttgaattc agcagctatt tcagtcaaat aagcatcggg atgagtttca
    15721 atatagatct ttaatttatc tctatcaact ttttcaatta atagtggaag ttttcttgaa
    15781 gttagtgtat tgaaacgttt ttatacagtg ctaaaatttg aagtgtagtg cagatgtatt
    15841 tgtaatagta ttatagcaat tctttggaga aggtcgagac aagataaaat aggtatcttt
    15901 gagatataag catgatttac tataaaatta ttgagttatt cataaaaatt tctctatcaa
    15961 atatcataat gcgtcgttta aggagatggt tacaatgcaa tatagttgtg gaaaaataaa
    16021 tattaatatc cctgatggat atggagacat taaggatata gtgttttcgg ctcatattat
    16081 agttaggtat aataatggac attgtggagg tatagatccg catataattg gactttgtaa
    16141 aaagcagatc agaaggatgt ctctatatcc tattttgata atcgtatcta gagattcaaa
    16201 ggttattgat gattataaaa atttagatat tgcctatgtt gattgtactc aatgttcaaa
    16261 taattttgaa actgccttac acgttaaaaa tattttaaaa ttattaaaaa tccaacttat
    16321 acactgtcat ggctactcaa ctaattattt tttatatatg ttaaaaaaac ttgacaagaa
    16381 tggatttgga aaggttaaga cagtgataac atgccatgga tgggttgaat ataacttaaa
    16441 aaagaaattc ttgacttatt ttgatttttg gacatattct atgggagatg cttttatttg
    16501 tgtatctgaa actatgaaaa aaagattgga gagtataata aaaaataaga agatagttgc
    16561 aataaataac ggaattaatg tatcaaatag cgatttagat gtagtaggtg ttcaagactt
    16621 taaaaaagaa ttttgtattc caaataacaa aaaaatcatt tgttatgttg gaaggttgga
    16681 tccagaaaaa aggccggata gattccttga atttgcagaa aaattatttt tagtcagaga
    16741 agatgtgatt tttattatgg ctggaaatgg tagtatgtgg gctgctttga aagaaaaaat
    16801 ttgccatttg aaatgtagag ataatttcag gttacttggt gaaatctatc cagctactat
    16861 agtttaccaa atatcggatt tattgtatat tccttatgat acagaaggaa tacctatgtg
    16921 tgtgttagag tcaatgtcac aaggaactcc ggttttagct agtaatgttg gcgggttaag
    16981 tgaaattatt gaacataggg ttgatggatt tttatttgag aaggaagatg ttgagggagt
    17041 gtgtgcttgt gctaattttt tactcaatga ttctgagtat ttgaaatata taggtgagaa
    17101 tagtaaatca aaaataagaa aacatttttc tgtgcaaaaa atgtttgtag aaactatgag
    17161 agtatatgat gaattattag agaagagtag tc
//
LOCUS       DAVKJD010000036        16894 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKJD010000036 DAVKJD010000000
VERSION     DAVKJD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16894
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.036"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(196..1830)
                     /locus_tag="VW157_001227"
     CDS             complement(196..1830)
                     /locus_tag="VW157_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552527.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAFRFPFEEMPSFFSTMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSIEAILGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFLPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPILTLLGLSLYLGLPIISMIYLIMAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLST
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1811..2560)
                     /locus_tag="VW157_001228"
     CDS             complement(1811..2560)
                     /locus_tag="VW157_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552528.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVADASQGH"
     gene            2739..4193
                     /locus_tag="VW157_001229"
     CDS             2739..4193
                     /locus_tag="VW157_001229"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9552529.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRSIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4283..6901)
                     /gene="alaS"
                     /locus_tag="VW157_001230"
     CDS             complement(4283..6901)
                     /gene="alaS"
                     /locus_tag="VW157_001230"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9552530.1"
                     /translation="MKQLSSAQVRQIWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DHGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6923..7408)
                     /locus_tag="VW157_001231"
     CDS             complement(6923..7408)
                     /locus_tag="VW157_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9552531.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7683..7922)
                     /locus_tag="VW157_001232"
     CDS             complement(7683..7922)
                     /locus_tag="VW157_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552532.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRHYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8175..9245)
                     /locus_tag="VW157_001233"
     CDS             complement(8175..9245)
                     /locus_tag="VW157_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9552533.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9242..10015)
                     /locus_tag="VW157_001234"
     CDS             complement(9242..10015)
                     /locus_tag="VW157_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552534.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(10012..10818)
                     /locus_tag="VW157_001235"
     CDS             complement(10012..10818)
                     /locus_tag="VW157_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552535.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10799..11956)
                     /locus_tag="VW157_001236"
     CDS             complement(10799..11956)
                     /locus_tag="VW157_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552536.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12116..13021)
                     /gene="murB"
                     /locus_tag="VW157_001237"
     CDS             complement(12116..13021)
                     /gene="murB"
                     /locus_tag="VW157_001237"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9552537.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13133..13885)
                     /locus_tag="VW157_001238"
     CDS             complement(13133..13885)
                     /locus_tag="VW157_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEU9552538.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13895..14626)
                     /gene="budA"
                     /locus_tag="VW157_001239"
     CDS             complement(13895..14626)
                     /gene="budA"
                     /locus_tag="VW157_001239"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9552539.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14768..16039
                     /locus_tag="VW157_001240"
     CDS             14768..16039
                     /locus_tag="VW157_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9552540.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFMAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LTRLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(16059..16874)
                     /locus_tag="VW157_001241"
     CDS             complement(16059..16874)
                     /locus_tag="VW157_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9552541.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQVGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
BASE COUNT         5083 a         3816 c         3324 g         4671 t
ORIGIN      
        1 ttaacgtttt tgaaattgaa aaatatccaa ataaataaac tataaacaac aaaaatagaa
       61 ctatgttata tttcttattc aaaacattcc tccctatata tttttgatta ccaatcttaa
      121 tcatttacaa ctacattcta acaaactata aaagcgtttg tcgaattgaa tttatcaagc
      181 aagcgaccaa ccagctcatc ttttttctat ttctgccaat atgcgtgaca ggtaataatg
      241 atagccaaaa atagcaagag caagcaggag gataagagct cctactccca agctgatggc
      301 aaggatagtg gagagagact gaaccaagaa tatgctccca attacaaggg ccatcaggat
      361 tgcactataa ataaacaata aaactatggc gactatgcca tttgaacgat tcaccaggtc
      421 cgtaatgcta ctccaattgg ttgacagatt tttaacgtcc ttaaagtaat ggtggcaaga
      481 aaggatgaca ctggcaagga tccatgccat aataaggtaa atcatcgaaa tgatgggcaa
      541 gcctagatat agagaaagac caagtaaagt cagaattggt aaaaaggact ggacggcata
      601 tataatccaa aatttcactt tcacataacg agcaaagtca aagggtaaac tcttaagaaa
      661 atcaacattt tccctctcca aggacaaggc aattgaatgc aggctggtga tattgttatt
      721 gacaactgct ataaagagag ctataaaaaa caagggtaac cagtatggag gatgaatgtc
      781 tggaactatc tgagaatctc ggattttgga aatcagaccg atcatcatga gataaggaag
      841 gaaagcactt gtaaaaagca ctgtaaccac gccagtaccc tgtcccaaga gggtgaggtg
      901 gtagcgtaaa accatacgga aaaagccctt tttagtagtt gaaatcctct ccttgctgcg
      961 acgttctttt ttgaccttct cctcactgtt aagcaggatc acgtcataaa aacgaggaag
     1021 gaccttcttt ttggtcagat aaagcaggaa gagagttagt cctatccaag cgagcagacc
     1081 caatatggct tctattgaaa aaggctccac tgctattttg taaaagatat gaagaggata
     1141 aaggagaaat ggaatgtctc taactttgtc aacaatactt ccaaaagtcg actgaagaaa
     1201 gaagataaat attaaaggta tgagaactcc tatcccaatc atcacattcg aaaaaataga
     1261 ctgatacttt ctgaagaccc tagtctgagc caagaaatgt actgccacta ccgtcactaa
     1321 agtaacagag acaaataata aggtcaagga cagtagcatc aaaggcaaac ccagccaaag
     1381 agaaggtgct agcctaatgt agaggaccag aaaataagct aggattggta caattccagt
     1441 tagagctggc aagaggacag acagaccttt ggccattata atctctgatt ctttaaaggc
     1501 atagggccta taggaggcca ggtccttact ctcataaaag acattgtaaa atgcagtgaa
     1561 agaagttgaa aaggccagca ccagtaaaat agcaaccatc gtactaaaaa aacttggcat
     1621 ttcctcaaaa ggaaaacgaa aggctatatt actaaaacag atgagcatca agagactgga
     1681 aaaaatgtaa gaacttagga ctctagcgga aacatttact tttttcccag gattcttagc
     1741 ctgcttcttt cgtagattag ccagattagc ttcttgagat gaataaagga tattgatatc
     1801 aactaatttt ttaatgacct tgagacgcat ccgcaacctc ctcttttcta ccagcaagac
     1861 taaggtagat actttccaaa gactggtctg ggtaatcttt tctcaagtcc tctacactac
     1921 cacaataaat caaatgcccc tttttcaaaa tggcaatccg atcacagact tgctctgcca
     1981 cctctaggac atgagttgaa aacaagactg tcttcccttt ttgtgcatgt tccttcatca
     2041 tctgtttcaa atcaaaggca gcctggggat ccaaaccagt caagggttca tccaagaccc
     2101 aaatatcggg atcagacaag agtgctccga tgacaaagac tttctgacgc attccgtgag
     2161 aaagggtttc aataacctga tagcgatttt cagcaaaatc aaaaacgttc aatagcctag
     2221 ctagactagc ctccaagtca gatctactca gatcatagga tgaggcgatc aattcccaaa
     2281 attcattggc cgttaagcgt aaaaataagt caggcgagtc tgctacgtag ccaatctttc
     2341 gtttaatagc caagcgattt tccgataact cctgaccgtc taccaaaata cgaccactgc
     2401 tgggtgaaat gatactgact agggatttta tagtggtcga ttttccagcc ccattatgac
     2461 caatcaagcc cataatctct ccattttcaa tctgcaaatt gagattactc aaggcctctt
     2521 tatcaccata caacttacta acattttgaa attcaatcat gggacaactc ctatctttat
     2581 ctgcattata tatcgctata ctagcacttt gatacaaaaa aatcaatact ttattttaag
     2641 tagttaaaat agtttccttc tatcttacgc aaacgtttgc gctaatagat actttatgat
     2701 agaataaaga acaagattga caagtaagag gaaacattat gcaaaatcaa acactcatgc
     2761 aatactttga atggtatctg ccccacgacg gtcaacactg gacgcgtctg gctgaaaatg
     2821 ctccacacct agctcatctg gggatcagtc acgtctggat gccaccagcc ttcaaggcaa
     2881 ccaacgaaaa agatgtcggc tatggggtct atgacttatt tgacttagga gagttcaacc
     2941 aaaaagggac tgtccgcacc aagtatggtt tcaaagaaga ctatcttcaa gccattcaag
     3001 cccttaaagc gcagggaatt caacctatgg ccgatgtagt tctcaaccac aaggctgctg
     3061 ctgatcacag ggaagccttt caggttattg aagtggatcc tgtagaccgt acagttgaac
     3121 ttggagaacc cttcaccatc aatggctgga ctagttttac cttcgatggt cgccaagata
     3181 cctacaatga cttccactgg cactggtacc acttcacagg tacagactat gatgccaaac
     3241 gccgtaaatc cgggatttac ctaatccaag gagacaacaa gggctgggct aacgaggaat
     3301 tggtcgataa cgaaaacgga aactacgact acctcatgta tgccgaccta gactttaaac
     3361 atcctgaagt catccaaaac atctatgact gggctgattg gttcatggaa acgactggtg
     3421 tagctggttt ccgtttggat gccgttaagc atattgactc tttctttatg cgcaatttca
     3481 tccgcgatat gaaggaaaaa tacggtgacg atttctatgt ttttggtgaa ttttggaacc
     3541 cagacaagga agccaatctg gactatctcg aaaaaacgga agaacacttt gaccttgtcg
     3601 atgttcgtct ccaccagaat ctctttgaag ccagtcaagc tggagcaaac tatgaccttc
     3661 gtagcatttt cacagatagt ctggttgaac tcaagcctga caaggctgtg acttttgtcg
     3721 acaaccacga tacccaacga ggacaagccc ttgagtctac cgttgaagaa tggttcaagc
     3781 cagcagccta tgccctcatt ttgttacgcc aagacggcct tccatgtgtc ttttacggag
     3841 actactatgg gatttcaggc cagtatgctc aagaagattt caaagaaatc cttgaccgcc
     3901 tcctagccat ccgaaaagat ttggcctatg gagaacaaaa tgactacttt gaccatgcta
     3961 actgtatcgg ttgggtacgt tcaggtgctg aaaatcaatc cccaatcgca gtccttatct
     4021 caaatgacca agaaaacagc aagtcaatgt ttgtcggtca agaatggact aatcaaacct
     4081 ttgtagattt acttggtaac caccaaggtc aagttacaat tgatgaggaa ggttatggac
     4141 aattccctgt ctcagctaga tccgtaagtg tctgggcagt caataccatc taatagctca
     4201 taatagccaa gctaggtcca agcggatttg gcttttttgt attcacaaaa agacctaccc
     4261 aaatggatag atctttactt gattacaatt tacctgctac tgcatccaac aattcttgga
     4321 tcttagcttg gttgcttcct cctgccatgg ccatgtctgg tttaccacca ccacgtccat
     4381 cgatgattgg tgctaattct ttgacaaggt ttcctgcatg aaggtctttt gtcttgcttg
     4441 ctacaaggac attgactttg tcaccgatag cggcaactag gacaagaaga tcagagtagt
     4501 ctttttgttt ccagttatct gcaaaggtac gaagggcacc ggcatcggat acagacactt
     4561 gactagcaat gtaacgatga ccgttgactt ccttaacatc tttgaagata tcacctgcgg
     4621 ctgcagctgc ggctttttct ttcaactcag cattttcttt ttgaagttga cgaagttgtt
     4681 cttgaagtcc ttctaccttg tgaggtactt ccttgacttg aggtgctttc aaggttgctg
     4741 cgacagcttt aagagcatcc tcttgttcac gataggcttc aaaggcttcc ttaccagtca
     4801 ctgccaagat acggcgagtt cctgaaccga ttccttcttc tttgacaatt ttgaagagac
     4861 caatctcaga agtgttgcca acatgagtac caccacaaag ttcaatagag tagtcaccga
     4921 tagtcacgac acgaacttcc ttgccgtatt tctcaccaaa gagggccatc gctcccattt
     4981 ctttagcagt gtcaatatcc gtttcaactg tcttaacttc aagagcttcc cagatttttt
     5041 cgttgacttg ctgttcaatc gcacgcaatt cttcagcagt tacagcttgg aagtgggtaa
     5101 agtcaaagcg aaggaattca acttcgttaa gagatcctgc ctgtgttgcg tggtttccaa
     5161 ggatattgtg aagggcagcg tgaagcaaat gagtcgcagt gtggtttttc atgacacggt
     5221 gacggcgatt gctatcaatt gccaaggtat attcttggtt caaggcaagc ggtgcaagga
     5281 cttcaactgt atgaagggct tgaccatttg gagctttctg aacattggtc acagtagcca
     5341 caaccttacc tgactcatcc aagatttgtc cgtggtcagc tacctgtcca cccatttcag
     5401 cataaaatga cgtttccgca aaaataagag aggcagttcc ttctgaaaca gcttctactt
     5461 ctgcattgtc cgccacgata gctaccaatt tagaagacaa ttggctagca ttgtagttga
     5521 agacactttc tacagtgatg ttttgaagag tttcattttg catacccatt gagccaccct
     5581 tgacagctga cgcacgcgcg cgttcttgct gttctttcat ggctgcttca aaaccttcac
     5641 ggtctacagt cataccagct tcttcagcga tttcttcagt caattcaact gggaacccat
     5701 aagtatcata gagtttgaag acatctgaac cagcgataac agattgacct ttttctttca
     5761 agtctgctac aatgccttgg gcaaagtgtt gacctgagtg tagggtacga gcaaatgact
     5821 cttcctcgct cttaacgatt ttttcaataa agtcacgttt ttcaagcact tctgggtagt
     5881 agctttccat gatttttcca acagttggaa cgagtttgta aaggaaaggc tcgttgattc
     5941 ccaatttttg accatgcata gaagcacgac ggagaagacg acgaagaaca taaccacgac
     6001 cttcatttcc tggaagggca ccatcaccga tagcaaatga aagagaacga atgtggtctg
     6061 cgataacctt gaagctcatg ttgtcgccat cttggtcata aaccttacca gacaatttct
     6121 cgacttcacg gataatcggc ataaagaggt ccgtttcaaa gttggtctta gccccttgga
     6181 taacagccac caaacgctcc aaaccagcgc ccgtatcaat gttcttatgt ggcaattcct
     6241 tgtattcgct acgaggaaca gcagggtctg cgttaaattg tgacaaaacg atgttccaga
     6301 tttcaatata acggtcgttt tcaatatctt ctgcaagcag gcgaagaccg atattttctg
     6361 ggtcaaaggc ttccccacgg tcaaagaaga tttctgtatc tggtccagaa ggtcccgcac
     6421 cgatttccca gaagttgtcc tcaattggaa tcaagtgact tggatccact cccacttcaa
     6481 tccagcggtt gtaagaatct ttatcgtctg gatagtaggt catgtaaagt ttttcagcag
     6541 ggaaatcaaa ccattcaggg cttgtcaaaa gctcataagc ccaagtgata gcttcgtcac
     6601 ggaagtaatc cccgatagag aagttcccca acatttcaaa catggtatgg tgacgcgcag
     6661 tcttccctac gttttcgatg tcgttggtac ggatagcctt ttgggcattg gtaatacgtg
     6721 gattttcagg gataatggtc ccgtcaaagt atttcttaag ggttgctacc ccagagttga
     6781 tccacaaaag agttgggtca tttacaggaa ccaaacttac tgatggttct actgagtgac
     6841 ctttggtcgc ccagaaatca agccatattt ggcgtacttg tgcactagat agttgtttca
     6901 tattgtctcc ttattcactt gtttaatgtg attggctttc cagcatttcc acatagtcaa
     6961 tcgcgacaca gagggaaatg actaggtctg cataagcgtc ctcaagaacc gttacggtat
     7021 aggtagaggt cagatggaag agttccttct taatttccgc aatcaactga tcgcgatcat
     7081 ccagcaattt gaaattcaaa tcccagatat tgccctcgat acgaagacct agattatcaa
     7141 actcatactt atctcgccag aaggtcaact tcttacgaat gacaaaactc gagccatccc
     7201 gaagctgaat ctcaaaacga ggaagcaagg tcaagatttc tttactgatc tgactgactt
     7261 gttcaccagc cgcatcatag atggtaaaag ttttgggaat cttaaaaaat gatccctcca
     7321 cctgataggc aatttctccc ctgtcatcct tgatagcgaa gcgttcgcct ccaagacgaa
     7381 acttttgttt gacaagaaat gttttcatca acacctccaa aaatcaaaag acaagctcat
     7441 atcacgaagg gcgaaaaacc gcggtaccac cttcattcaa tgaacttgtc attctcttgt
     7501 tcttatgcaa ttgtatgatt gagtagcatg acttcctagc ttagatggct cgcagcaccg
     7561 ccatttctct ggactaagac aagtgaaaat caattctcaa ctttcttatt ataacgtttt
     7621 tttaagcttg cgtcaactgg aaatgatctc cgttgaatta gaccaattcc ctacatctct
     7681 gattactttt tcaggatata ttttttctta ctgccatttt tctttttatc ccaaattttc
     7741 atattactaa acacagctac tagaatattt ccaaatataa aggtgcctat cacccaatat
     7801 atggactcag ttgttaggta gtgtcgatcc aagccatcct ttaaatggaa tagtatagca
     7861 gtttggttaa caatcataaa ggttggccag aaactttttt tgaaaaaagt agacattttc
     7921 attatttgtt gccgctttct gtaaggttaa tactcaataa aaatcaaaaa gcaaactagg
     7981 aagctagcct caagctgtac ttgagtacgg caaggcaacg ctgacgtggt ttgaagagta
     8041 taggcttagt atactactag gcaagcaaat aaacaaataa acaactagaa tagaaaaaga
     8101 tagggctcta aaaactgact tctattcctt aaaaacgaac cagcttgact gattcgtctt
     8161 cttacgttta tctcctactt ccgatacatt ttaaactgta ggaagaggtc gctatatttc
     8221 cctgtccatt tatggtcaaa tttctcataa acttctaggt gtttcatggt ttcaacatcg
     8281 ggatagaagg ccttatcttc ctttgtttcc tctgggagca attccttcgc tggtaggttt
     8341 ggtgttgaat agccgacata ctccgcattt tggagagcat tttcaggttt caacataaag
     8401 ttgataaagg cataggctga gttttggttt ttaactgttt tgggaatgac catattgtca
     8461 aaccaaagat tgctggcctc tgtcggtacc acataacgta gattttcatt tttttctaac
     8521 atttggctgg cttcaccaga gaaggtcacg ccgattgcaa cattattctg aatcatatag
     8581 cccttcatct cgtccgcaac gatagccttg atatttggag tcagtttgta gagcttatcc
     8641 actgtctctt ccaactgctg cagatccttg gagttgaggc tgtagccgag ggaattgagt
     8701 cctagtccca gcacctcacg cgccccatca aagagcatga tagaattctt atactccggc
     8761 ttccaaaggt catcccaatg ctcaggcgct tcatctacca tggtttcgtt gtagacaatt
     8821 cctaaggttc cccagaagta agggatggag aatttattac ctgggtcaaa ggactggttg
     8881 agaaactctg gtccgatatt ttcgattcct tcaatttttg aataatcaag cggaaccaag
     8941 aggtcttcgt ccttcatctt gttaatcatg tattcacttg gaatggcaat atcgtaggtc
     9001 gttccaccct gctttatctt agtgtacatg gcttcgttgg agtcaaaagt ctcgtactga
     9061 acttgaattc ctgtttcttc tgtaaactga gtcaagagtt caggatcgat atagtctccc
     9121 cagttataga taaccaattt ttgactatct cgactattga ttttactatc taaatgagtc
     9181 gcaattcccc acaagacaag gataatcgct gcaattcctg ctaaaaatga atagattttt
     9241 ttcatgcttg ctcctccttc tcacgagaga taaagtaata acctacaact aggataatac
     9301 taaagagaaa gactagagca gacagggcat tgatttctaa ggaaatcccc ttgcgagcac
     9361 gagagtaaat ctcgactgat agggttgaaa agccatttcc tgttacaaag aaggtcacgg
     9421 caaaatcatc cagcgaatag gtgaaggcca tgaagtaacc tgcaatgata gacggagtca
     9481 ggtaaggaag catgatttcc ttgaacatct gaaattgact agctcccaag tcataggccg
     9541 catgaatcat gtcgccattc atttccttga gtcgaggcaa gaccatcaag accacgatag
     9601 gaatggagaa ggccacgtga ctagatagaa cggtcaaaaa gccaagtgaa aacttgagtt
     9661 gggtaaagag aatcaagaag ctagcaccaa tcataacgtc aggcgcaacc atgaggatat
     9721 tattgagtga tagaaaggct tcttggtatt tcttacgaga ctggtagatg taaatggcac
     9781 caaaagtccc gataatggtc gctatcaagg ctgataggaa ggccaagaaa aatgtctgag
     9841 ccaaaatcag catgagtctc ccatctccaa acatggtttc aaagtgagtc cagctaaaac
     9901 ctgtaaagct attcatatca tcaccagcat taaaggcata gccaatcaag taaaagatag
     9961 gcaggtagag gaccagaaag accagtccca gataaaggtt ggcaaatttt ttcatcgttc
    10021 tctcctttcc ttagtcaccc acatggtgat gaacatggtc aggatgagaa tcacaccgat
    10081 ggttgaaccc ataccatagt tgtcattggt tagaaaattc tgctcaatag ccgtccccaa
    10141 ggtgataacg cggttcccac caatcaaacg ggtcagcatg aagagactca aacttgggat
    10201 aaagaccgac tgaaccccac ttcgcacacc gttcatagat agagggaaga tgacatgacg
    10261 gaaggtctcc cacttagttg caccaaggtc ataactagca ttgatgagat tattatccat
    10321 atcgtctaag acattgaaaa tcggcaaaat cataaagggg agctcgatgt agcttgcgac
    10381 aaagataaag gaaaaatcgg taaaaagcaa ctgttgtgaa ccaattccga taaattccaa
    10441 gaattggtta atagagccat tttgaccaaa aatcccgata aaagcatagg ctttaaggag
    10501 caaattgatc caggtaggaa ggataatcag catgagccag agttgacggt gcttgagacg
    10561 ggtcaaaaag agggccgtcg gataactgat aagcagtgtc acaaaggtca caatgcctgc
    10621 ataaagcact gagttgaaac tcattttgag gtaggtcaag ttttgtgacg caaagtaaga
    10681 tttgtaattt tctaaactga actggccttc gatgttgaaa aaggattgac cgaaaatcaa
    10741 gaccaagggt gccaatacaa agagcgcaat ccaaagcatg tagggtacta caaagagttt
    10801 agagcttgtt ttcttcatct ctttcctcct cgattgcatt gatcaaacct gcttcttgct
    10861 cttcgatttc tacgtactcc tcaatacgag catcgaactc ttcttcggtt tcattgagac
    10921 gcatgatgtg gatgtcttct ggttcaaagt ccagaccgat ttcctcaccc acgatagcct
    10981 tacgggttga gtggatcatc cattcatttc caagttcgtc ataggcgata atttcataat
    11041 gaactccacg gaaaagctgg gtatcgacct taacttggag cttgccttct tcaggaaggg
    11101 taatgcgcaa gtcctctgga cgaataacga cctcaacagg ttcatttggc ttcatcccac
    11161 catcaaccgc ttcaaagcgt ttgccgttaa attcgaccaa gtagtcctca atcatggtac
    11221 ctggcaagat gtttgactcc ccgataaagg tggcaacaaa gtggttgatt ggctcatcgt
    11281 agatgtccac aggggttcca gactggacaa tctcgccatc attcataacg aaaatccagt
    11341 cactcatggc aagagcttct tcctgatcgt gagtgacaaa gacaaaggta atgcccaatc
    11401 gttgttgtaa ttcacgcaat tcgtactgca tgtctgttct caatttcaag tccagcgctg
    11461 ataaaggctc gtccaacaag accacacggg gttggttgat gatagcacgg gcgatggcca
    11521 cacgctgacg ttgtcctcca gaaagtttgc ggatggaacg tttttcataa ccttccaact
    11581 gaaccatctt gagaacttcc gctacacgct gctcgatttc tttcttatca attttacgca
    11641 agcgaagtgg aaaggcaaca ttttcaaaca cattcatatg tgggaacaag gcataggatt
    11701 ggaagacggt atgtacgtcg cgcttgttgg ttggaatatc attgatacga acaccgtcta
    11761 gcatgatatc tcctgtcgtc gcatccagta aacctgcaat aatgtttagg atagttgatt
    11821 tccccgaacc agatgcacct agaagggtgt agaatttccc ttcttccaac tcaaagttga
    11881 tgtctttgag aaccttggtg ttgctgtctt caaaaacttt agagacgttt ttgaattcga
    11941 taattggttt tttcaattgg cataaattcc ttctttttca tagattaacc gatcggggct
    12001 ctgtcaggtc cccactacct cttgcaggga gtaaaaccac ctgcatacat cttcgctacc
    12061 gataggcttt cacccccttt ccatgacata gcagcagctt ttcaaataca gcttcctact
    12121 tgctttcacc caagatccgg acttctcttt caagcgtaat acctgagtgt tccttgactt
    12181 tttcgataac cgattggatc aagtcctcgt agtctttggc cgttccatct gcgacattga
    12241 tcataaatcc tgcatgcttt tctgacactt ctacgccacc gatacgatag cctttcaagc
    12301 cagcttctga aattaactga cctgcaaaat gcccgactgg acgcttaaag accgagccac
    12361 aagatgggta ttccaaaggt tgcttgagtt cacgtaggtg cgtcaagcgg tccatttcct
    12421 gcttgataac ctgatgggtt cctggggcta gggcaaattt aactgacaag acaactgcac
    12481 cagactcctg aatagctgaa tggcggtaac caaaagccaa gtctttagca gacagggttt
    12541 cgatttctcc atccttggtc aagaccttac aagactgcaa gatgtgagca atctcgccac
    12601 cataggcacc cgcattcata aagacagcac cgccaacgct tcctggaata ccacaagcaa
    12661 actcaaagcc agtcaaacta tgacggaggg caatgcgagt tgtttcaatc aagttagccc
    12721 cagcttctgc ttcaatggta tagccatcaa cagaaacgtt attgagcttg tcacacaaga
    12781 tgacaaatcc acgaatccca ccatcacgaa cgatgatatt gcttgcattg ccaagaacca
    12841 tccaagggat attttcttgg ttggcaaatt tcacaaggcg cgccaactca aaacgatttc
    12901 gtggaaagac caaataatca gcctctccac ctacttttgt ataactatag ctatgcaagg
    12961 gttccttaaa acggatatca attccttcta agatttcaag cattttttct cttacagaca
    13021 tgtcactctt ccttttacaa aattcattcc attataccat ttttagagac atttgacgac
    13081 cataaaaata ccttgtttgg attttgcata agaaaaagag gttcccccct ttttatgatt
    13141 ttttacaaaa gatttccttg gttccatagg caaccagaac gagctccagt gctagaatca
    13201 cttcaaccaa gactggattt gtcaaccagc ctacttggaa aagagatggt gccagatcaa
    13261 agaaggcatg caagccatag gctgctagga gataaatcca tttcttctgg cgaacagctt
    13321 ggtaaaccca aactgtcaaa agtaattgga aaccaagcgc caagattcgc tcaaaaccaa
    13381 gcaaataaat ctgccagacc gaaagtgact gaatggtttt taacatattt tcagacagta
    13441 attgcataac ctgtggattc tgagtttgaa ctgccgaaag aacgatgtaa agattgagta
    13501 aactaataag gcctagaaaa atcaactcca agccaccatg ccctaggcca taggccaagg
    13561 catctgcctt ttccaaactt ctctttttct ccaaccattt gaagaaaaca agacgagccg
    13621 tttcctcaaa aaatgctgcc atggctaggc catagataat atagataagc ggatgatctt
    13681 gcaagagggc aatactaccg tctttttgag gatgcaaaat caagatatgc accagttttt
    13741 ccaaaatctg cgaagagaca aagaaagcga ctgcacccaa gcccaagaca gctagattaa
    13801 tctggtattt ctttttggca aaccaaatgc ttcctatcag aaaagctagc aacagcaaca
    13861 tggtaataat gatatgaatg gtcattttct tctcctattc tgccttttca atatcttttt
    13921 tcatctcgtc aacattgaac ttagcaaaca agtattgacg gtcttggact gggaaacgtt
    13981 ggtccaactg gtcaactgct cccacctcga taatgccttc cttgatgaca aaatccatga
    14041 catgtccacc gaaggtcaaa tcatctgata tgaagtgcag atggtagcct gccacactga
    14101 ccccatggaa aatctcaggc gtccagaaac caacaatagt ccccgcaaca ttgtcacgac
    14161 tatattccgg ttgatgggtt gcgacatcag caaacttggt atcgggtgtt gacttaggaa
    14221 tcatacgcac atgcatatgt gaaaattccc cccgaatctt gatagagcgg aaaagatttt
    14281 ccccatcata ataagactca attcgttctt tcaattcctt gtctgtcatc tcaaagcgct
    14341 ggcggaaaat gacctctgcc tgatgcggta ccactgcagc gtaaggaata agggcatctg
    14401 gtgacacttc cacaatttct ggctggtctc ctgacccttt ggcctgataa gccttgccat
    14461 ccaagacaat caattcccca tcaatggaat ccaaggttcc caaaccaagg tcaccatgtt
    14521 ctagcaattc tcctactgtc atagtaccac cataaaggcc agccatcaag gctccaagag
    14581 tattgtattg aaataatttc actggttcct gcacgttctt atccattcac tttcttgtct
    14641 gttattctat aaatcttact cctaagtata ccacatttgc ccctagatgt gaacgagaga
    14701 aacgctctag acattgccaa gaaggaaaaa aaagggtaca atgtaacaaa atcaagggag
    14761 gtctggaatg aagaaacaaa gcaagtacaa agaggtcgtt tcctatctga aaaatggtat
    14821 cgagtctgga cgatttccga cgggtagtcg cctgccttct atccgtcaac tgagccttga
    14881 ctttcactgc agcaaggaca ccattcaacg agccctgctg gaattacggc acgaacaata
    14941 cctctatgcc aagcctcaga gtggctacta tgtattagaa caagggcaac atcaagacct
    15001 agaaatcgag gttaccgacg aacatgccag tgcctatgac gatttccgac tctgtgtcaa
    15061 tgaaaccttg attggccgag aaaactacct cttcaactac tatgacaatc aagaaggatt
    15121 agaagaccta agacagtcca ttcacaaact cctctttgag caagctctct actgcaaggc
    15181 taaccaacta gtactgactt ctggaaccca acaagccttg tttatcctct ctcaaatatc
    15241 ctttcctaga caagccaagg aaatcttggt ggaacagcca acctaccatc ggatgaatcg
    15301 cctcttgatt gcacaggggc tggactatca aacgattgaa cgaggcattg atgggattga
    15361 cttggaggag ctggaaggcc acttcaaaac aggaaaaatt aagtttttct acaccattcc
    15421 ccgatttcac tatcctctgg gacattccta ttctgagcaa gacaaacgat ctattcttaa
    15481 cttagctgcc aagtatgatg tctatatcgt agaggacgat tatctgggtg atttggactc
    15541 caagaagggc caaaccttcc actatcttga tacagaggag cgtgtcattt atatcaagtc
    15601 cttctcgacc agcctttttc ctgcccttcg tattacagca ctcattcttc caaatgctat
    15661 caaagaagca tttatggcct acaaaaatat cctagactac gacagcaacc tcattatgca
    15721 aaaggccctg tcactctata ttgacagtca attgtttgaa aaaaatcgtt tgactcgctt
    15781 gaccaatcat gaatcttacc aaaagcaaat cgaggaaagg ataactaaaa caccttgtcc
    15841 ccttcctcat tatcccctac acgatggttt attgctagac ctgagacagt atcctaaaat
    15901 cgccagtctc aaacacagtc aactgggctt ggacttcttt gaagaggcct atttaagcac
    15961 ctgtccttat caatttgcca aggtgtccct agacaatctg gaaaatgttt taaactattt
    16021 aaaagcagaa ttggaatgac atccaactct gctttttctt attcttcaac ttcagttaga
    16081 cttgctgctt tttcaagata gctttggtaa gttccttcat ccttaagttt ttggataacc
    16141 ttgtctacca cttctttcaa atcatcacta ttttttctaa gagcaacggc attggcgtcg
    16201 ccgtccttca tcttcaagct gacagttgcg acagctaagc cagcgttttt agcagcataa
    16261 ctaagtgcaa caggctcatc catatgaaca gcatctactt ttccaacctg caattcattg
    16321 actgcttcac ccatattagt tagggaagtt aattgagctt ttggcaattg ttccttgacc
    16381 attgattctg gaacagtccc tttttgggct gcaatattag cactttctag gctagttaaa
    16441 tccttgtatt tttccacatc agccttacga accaagaaac taatcttgtt ttcatagtat
    16501 gggattgaaa aatcaaagac ttcttttctc tcgtcagtag cactaattcc tgcaactgct
    16561 aggtcagcct taccagtttg aagactggtc aaaacattgt caaaactcat gcttgagatt
    16621 tccaacttaa ccccaagttc atcagcgata gcctgagcca tgtcgatgtc tgcaccgact
    16681 acctggttct ttccatcaac caatgattga aattcaaagg gtgcatagtc aggactggtt
    16741 gccacaacta atttcccttt ttgtttgata gcatccacag cagactgaga accattagaa
    16801 cctgactgac aagctactaa gaagaaactt gcaagaaaat tacataaaac aaatatccat
    16861 ttttttgatt tcataaataa acaacctctc tgtt
//
LOCUS       DAVKJD010000037        16458 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKJD010000037 DAVKJD010000000
VERSION     DAVKJD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16458
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.037"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            88..1848
                     /locus_tag="VW157_001242"
     CDS             88..1848
                     /locus_tag="VW157_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9552542.1"
                     /translation="MSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIY
                     WNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENSIFDMKRSTNEFIG
                     RSAMVTKLLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNYKIIELVSKTYP
                     ELEPSKLGTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGK
                     TYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKD
                     FCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSG
                     VPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKF
                     VFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIG
                     TMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIEN
                     VQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAF
                     ELTNNEQTEPQDTGDNDADN"
     gene            1832..3151
                     /locus_tag="VW157_001243"
     CDS             1832..3151
                     /locus_tag="VW157_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000984591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9552543.1"
                     /translation="MMRITDNQHRIAKEGFVAEYPKLSQALLDRTLDNLSKEDNIFIF
                     PNDLKNSPDLEKDQKIFEIVNQELKTGNVIGFLGYGQERLTISSRFSIKGDDYFLHYL
                     LQKVLHINLTSLDVALSCEERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKG
                     VIDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLIRHTIEYIKNQKSIGQGVLDSLLT
                     SRENVAEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRKLQELCLMILNQEKHGL
                     GYQDQKIHGILFDVAWLWEEYVHTLLPKDFIHPRNKDKTDGISVFSSRERKVYPDFYN
                     RDLKIVLDAKYKKLEDTEKGINREDLFQLISYSYILKAEKAGLIFPSEKKAVDNEIGK
                     LAGYGALLKKWSIQIPQESSSYSEFCEMMENSEEIFKRNITKELGRK"
     gene            3326..4312
                     /locus_tag="VW157_001244"
     CDS             3326..4312
                     /locus_tag="VW157_001244"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9552544.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     TGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4376..4447
                     /locus_tag="VW157_001245"
     tRNA            4376..4447
                     /locus_tag="VW157_001245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4409..4411,aa:Arg,seq:ccg)
     gene            4630..5328
                     /locus_tag="VW157_001246"
     CDS             4630..5328
                     /locus_tag="VW157_001246"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9552545.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFNQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVIIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5319..8858
                     /gene="smc"
                     /locus_tag="VW157_001247"
     CDS             5319..8858
                     /gene="smc"
                     /locus_tag="VW157_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9552546.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKASQQKDELETAKVQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRVGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLGSEEAALKTLQDQMAALTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQESLATIASEKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEEVAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8855..9649
                     /locus_tag="VW157_001248"
     CDS             8855..9649
                     /locus_tag="VW157_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9552547.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            9649..10467
                     /locus_tag="VW157_001249"
     CDS             9649..10467
                     /locus_tag="VW157_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9552548.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            10471..11748
                     /gene="ftsY"
                     /locus_tag="VW157_001250"
     CDS             10471..11748
                     /gene="ftsY"
                     /locus_tag="VW157_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9552549.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVEPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETIE
                     ENNSEVLEPERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKGGNYDERIHFQ
                     AGLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(11794..13281)
                     /locus_tag="VW157_001251"
     CDS             complement(11794..13281)
                     /locus_tag="VW157_001251"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9552550.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDTEHYIALRQLQTELN
                     DKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYASASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDESGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFASGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFSAKWTWQPDIAYRQDGRLE"
     gene            complement(13430..14170)
                     /locus_tag="VW157_001252"
     CDS             complement(13430..14170)
                     /locus_tag="VW157_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9552551.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14170..16335)
                     /locus_tag="VW157_001253"
     CDS             complement(14170..16335)
                     /locus_tag="VW157_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9552552.1"
                     /translation="MKKKFLAFLLILFPTFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
BASE COUNT         5134 a         2944 c         3622 g         4758 t
ORIGIN      
        1 ttaaataagg caactgttgg tgaaaagttt aatgataaat taaaagaaga gtttctccag
       61 gaatggcctt tggataggat tttaacaatg tcaatcgatg aatatgtaat aggaaaggga
      121 cagcaaaata agtctttatg ctacgctctt gagaagggaa aatacaaaaa tctatttctt
      181 ggaatttctg gtggctcagc ttcaaaattt ggtatttatt ggaataaaaa aacaaacaaa
      241 tataaagatc aagctaataa tgagatttca gagttggatc agcgattttc aaaattaaaa
      301 tcagatttgt atgaaattat caaagaaggt attcgtttta actttgaaaa ttctattttt
      361 gatatgaaaa gatcaacaaa tgaatttatt ggtcgttctg ctatggtgac aaaattactt
      421 tgtatctatt ctgagggaga tcctttcttt ggtgtaaata ttaatagtca gaaagaattt
      481 tggaaccact ttgtttctca gacaaatcaa ggtggacctt atctgcaaaa ttataaaata
      541 attgaactgg tgtccaaaac ttatcctgag ttggagccat cgaaattagg aactatgctt
      601 tttgagtatt ctaagctttt tatggaaaat aaggaagaca atagtacaat ggattcatca
      661 aacaattttc gtcatcaatt aactcaatct ctattaaagt ctccaaacct tatccttcgt
      721 ggtgctcctg gcacaggaaa gacttatctt gctaaagaaa ttgccaaaga attaacggat
      781 ggcaacgaag accaaatcgg atttgtacaa tttcacccat catatgatta tacggatttt
      841 gtagaaggtt taagaccagt atcaaatggg gatggagcta ttgagtttag gctacaggac
      901 ggtattttta aagatttttg tcagaaagca aaagaaaccc aattgattgg aggacaagat
      961 aattttgatg aggcttggga ttcttactta gaatatataa atgttgctga agaaaaagaa
     1021 tatataacaa aaacatctta cttatctgtt aatagtagac aaaatttgtc agtaaattat
     1081 gatagtggtg ttccaggatg gtcactacct agcaaatatg tttacgagtt gtataaagat
     1141 aaaaattata ataagcaaga atactacaaa agtggtggaa aaactgtcct agaaacattg
     1201 agaaagagat ttggtttgaa agactatgtt tccccaacag aaattgatac tgataagaaa
     1261 ttcgtcttca tcatcgatga aatcaatcgt ggggagattt ctaagatttt tggcgaactc
     1321 tttttctcta tcgaccctgg ctatcgtggt gaaaaaggaa gtgtttctac ccaatatgca
     1381 aatctacacg aaactgatga aaagttctat atccccgaaa atgtttacat catcggaact
     1441 atgaatgata ttgatcgttc agtggatacc tttgattttg ctatgcgtcg tcgttttcgt
     1501 tttgttgaag ttactgccga gggtcaagtt ggcatgttgg ataaagagct gaatatccat
     1561 gcagaagaag caaaaattcg tctaagaaac ttgaacgctg ctatcgaaaa tgttcaggaa
     1621 ttaaacagtc attatcatat tggaccaagt tattttctta agttgaagga tgtagatttt
     1681 gactatgaat tactctggtc tgattatatt aagcctctcc tagaagacta cttgcgaggt
     1741 tcttatgatg aggttgaaac tttggaaact ttgaaaaaag catttgaact gacaaataat
     1801 gagcaaacag aaccgcaaga tactggtgat aatgatgcgg ataactgata atcagcatag
     1861 aattgctaag gaaggttttg tcgcagaata tcccaaacta agtcaagcac ttcttgatag
     1921 aacactggat aacctttcta aagaggacaa tatctttatt tttccaaatg atttgaagaa
     1981 ttctcctgac ttagagaagg accaaaaaat ctttgaaata gtcaatcagg aactcaagac
     2041 aggaaatgtg attggttttc tgggctatgg tcaggagaga ttaacaatct cctctcgttt
     2101 ttcgattaaa ggtgatgact attttttaca ttatctttta caaaaggttc ttcatatcaa
     2161 tctcactagt ttagatgtag ctttgtcttg tgaagagagg ctttatcaac ttttgatgta
     2221 tctcttcccc aagtatctac aagctgctat tcgaaaaggt ctttataagg aataccatcg
     2281 attttctcat aacgacagtc atgttaaggg agtgattgat gtaagaaacc atctcaagaa
     2341 aaatcttcct ttcacgggaa atattgccta cacaacgaga gagttcacct atgataatcc
     2401 cctcatgcag ttgattcgtc atactattga atacattaag aatcagaaaa gcattgggca
     2461 aggggttctt gatagtctct taactagtcg tgaaaatgta gcagaaatcg tgcgtgtaac
     2521 gccatcttat aaactagctg atcgtgcgaa gattattcgg ggaaatcaat ctaaacctat
     2581 acgtcatgca tactttcacg agtacagaaa gttacaagaa ctttgtttga tgattctaaa
     2641 ccaagaaaag cacggtttag gctatcaaga tcaaaaaatt catggtattc tttttgatgt
     2701 tgcctggctt tgggaagagt atgttcatac tttgttgcca aaagatttca tccatccacg
     2761 aaataaagat aagacggacg gaatttcagt attttctagt cgagaacgaa aagtttatcc
     2821 agatttctat aatagagatt taaaaattgt gttagacgct aaatataaaa agcttgaaga
     2881 tactgaaaaa ggaataaacc gtgaggactt gttccaactg atttcctatt cttatatttt
     2941 aaaagctgag aaggctggcc ttattttccc tagtgagaaa aaggcggttg ataatgagat
     3001 aggaaagcta gcagggtacg gagctttgtt gaagaaatgg tctatccaaa ttcctcagga
     3061 gtcttcatct tatagtgaat tttgtgaaat gatggaaaac tcagaagaaa tttttaaaag
     3121 gaatattact aaagaactgg ggagaaaata atctctcctc tttcttttgt ttttgataca
     3181 agtaaaatcc gaacaatata atttaattca cgaaattatt tgacaaaaac tgtactttgg
     3241 tttataataa attaaggaaa cgtcggaaaa ggcgtagtga tgcgagagca taggtaggtc
     3301 attacaaaag aaacgagaca tcgatatgtt aaatgaattt ccaatttttg attacgaaga
     3361 tattcaattg attccaaata aatgtgtcat taaaagccga gcagaagcgg atacaagtgt
     3421 cactttagga aatcacacct ttaaactacc tgttgtgcca gcgaatatgc agacgatttt
     3481 ggatgaaaat gtagcagagc aactggctaa aggtggttac ttctacatta tgcaccgttt
     3541 tgatgaggca ggacgcattc cttttattaa acgcatgcac gatcaaggac tcattgcttc
     3601 tatctctgtc ggtgttaagg actatgagta tgattttgtt agccagctca aggctgatgc
     3661 tcctgagtat atcactattg atattgctca tggtcatgcg gatagcgtga tttctatgat
     3721 tcaacacatc aagaaagaat tgccagatac ctttgtcatt gctggaaatg tgggaacacc
     3781 agaagctgtg cgtgaattgg aaaatactgg tgcggatgct actaaggtcg gaatcggtcc
     3841 tggtaaggtt tgtatcacca aggttaagac tggttttggt acaggtggtt ggcagttggc
     3901 tgctctacgc tggtgtgcca aggctgcgcg taaaccgatt atcgctgatg gaggaattcg
     3961 tactcacggt gatattgcca agtctatccg cttcggtgct agcatgatca tgattggttc
     4021 cctctttgca ggacacattg aaagtccagg gaaaacgatt gaagtcgatg gtgaacagtt
     4081 caaagaatat tatggttcag cctcacaata tcaaaaaggt gcttacaaaa atgtggaagg
     4141 caaacgtatc ttgcttcctg ctaaaggtca tctgcaagac actttaactg agatggaaca
     4201 agaccttcaa agtgctattt cttatgcagg tggacgtcag gttgctgacc ttaaacacgt
     4261 tgattatgtg atcgtgaaaa actccatctg gaatggggat gcttcccact aagacggggt
     4321 ctttgaccaa aaaacttgtt attagagcaa atttctgtta taataaaaca agtttccacc
     4381 cttagtgtaa tggatatcac gtaagattcc ggttcttgag atgggggttc gattccctca
     4441 gggtggatgt aaacatccta aaaagccttt aatagggctt ttttctttat cctgacccaa
     4501 atttgccgct aaaactgaga atctctgtct aaagaattcc caaatgcttt ccagtcccgt
     4561 tttagagtga ctcagggggc tttttttgat ataataaaaa ggactattat cagttagaaa
     4621 gaggttggta tgaaagaatt acaaactgta ctaaagaatc attttgcaat cgaatttgca
     4681 gacaaaaatt tactggaaac tgcctttact catacgagtt atgccaatga gcaccgcctc
     4741 ttaaaaattt cacacaatga acgcttggaa tttttaggag acgctgttct acagttattg
     4801 atttcagaat atctatataa aaaatatcct aaaaagcctg aaggtgacct atcaaaactc
     4861 cgtgctatga ttgtccgtga ggagagttta gctggttttg cgcgtgattg ccagtttaac
     4921 cagtttatca agttgggtaa aggggaagaa aaatcaggtg gtcgcaatcg tgacaccatt
     4981 cttggtgatg cctttgaagc ctttcttggt gcccttcttt tggataagga tgtggccaag
     5041 gtcaaggaat ttatctatca agtcatcatt cctaaggttg aagcaggcga gtttgagatg
     5101 attacagact ataaaaccca tctccaagag ttgcttcagg tcaatggtga tgtggctatt
     5161 cgttatcagg tgatttctga aacagggcct gctcatgata aggtctttga tgtagaagtt
     5221 cttgttgaag gtaagagcat cggtcaaggc caaggtcgtt ctaagaaatt agcagagcag
     5281 gaagctgcca aaaatgccgt tgagaagggg ctggattcat gtatttaaag gaaatcgaaa
     5341 ttcaggggtt caagtctttt gctgataaga ccaaggtcgt ttttgaccaa ggtgtgacgg
     5401 cagttgttgg acccaatgga tctggaaagt ccaatattac agaaagtctg cgttgggctt
     5461 tgggggagtc tagtgtcaag agtctccgtg ggggcaagat gccggatgtc atctttgctg
     5521 gaaccgaaag tcgcaaaccg ctcaattatg cttctgtagt tgtgactctg gataatcatg
     5581 acggatttat caaggatgca ggtcaagaaa tcagggtgga acgccatatc tatcgtagtg
     5641 gagatagcga atacaagatt gacggcaaga aagtccgtct gcgtgatatt catgacctct
     5701 tcttggatac tggattggga cgagattcct tctctattat ttcccaaggg aaggttgagg
     5761 agatttttaa ttccaagcct gaggaacgac gagctatttt tgaagaagct gctggagttt
     5821 taaaatacaa gactcgcaga aaagaaaccg agagtaaact gcagcaaact caggataatc
     5881 tggaccgctt agaggacatt atctacgagt tggataatca aatcaagcct cttgaaaagc
     5941 aagctgagaa tgcccgtaag tttttagact tggaaggaca acgtaaggct atttatttag
     6001 atgttctggt tgctcaaatc aaggaaaata aggctgaact agagtcgaca gaagaagagt
     6061 tggctcaggt tcaagaactc ttgatgagtt attaccaaaa gcgtgaaaaa ttagaagaag
     6121 aaaatcaaac tcttaaaaag caacgccaag atttacaggc tgaaatggcc aaagaccaag
     6181 gcagtttgat ggacttgact agtctgatta gtgatttaga aagaaaatta gccctatcga
     6241 aactggagtc cgagcaagtg gccctgaatc aacaggaggc acaagctcgt ttggctgctt
     6301 tggaggataa gagaaattca ctcagcaaag aaaagtatga taaagaaagc tctttagctc
     6361 tgttagaggg aaatctagtc caaaataatc aaaaactcaa tcgtttagaa gctgaattgc
     6421 tggctttctc agacgatcct gatcagatga ttgagctctt acgtgaacgc tttgtagctc
     6481 ttttacaaga agaagcggat gtctcaaacc agttgacccg tattgagaat gagttggaaa
     6541 atagtcgtca gctttctcaa aaacaagcag atcaactaga aaagctgaaa gagcaattag
     6601 ctacagctaa agagaaggct agtcagcaaa aagacgagct tgaaactgcc aaggtgcagg
     6661 ttcagaaatt attggctgac tatcaagcta ttgccaagga gcaagaggag cagaaaactt
     6721 cctatcaagc tcaacaaagt caactctttg accgtctgga tagtctcaaa aacaagcagg
     6781 ccagagctca aagtttggaa aatatcctga gaaatcatag taacttttat gcaggtgtta
     6841 agagtgttct ccaagaaaaa gatcgcctag gtgggattat tggtgcagtc agtgagcatc
     6901 tgacctttga tgtttattat caaactgccc tagagattgc cttaggggca agtagccagc
     6961 atatcatcgt agaagatgaa gagtcggcaa ccaaagctat tgatttcctc aaacgaaaca
     7021 gagtcggtcg tgcaaccttt cttcctttga ccactattaa ggcgcgtacg atttctagtc
     7081 agaaccaaga tgctatcgct gtaagcccag gtttccttgg gatggcagat gagttggtga
     7141 cttttgatac tagactggaa gccattttca agaacttgct agctacgacg gctatctttg
     7201 ataccgtaga acatgcgcgt gaagctgctc gacaagttcg ttatcaggtt cgtatggtga
     7261 cattggatgg gacagaatta cgcacgggtg gttcctatgc gggtggtgcc aatcgccaaa
     7321 ataacagtat tttcatcaag ccagaactgg agcaattaca aaaagaaatt gctgcagatg
     7381 aagcaagctt gggttcagaa gaagcggctt tgaagacctt gcaagaccag atggctgcat
     7441 tgacagaaag attagaagcc atcaaatctc aaggagagca ggcacgtatt caggagcaag
     7501 gcttgtccct cgcttatcag caaactagtc agcaagttga agaactggaa actctttgga
     7561 aactccaaga agaggaaata gatcgtcttt ctgagggaga ttggcaagcg gataaggaaa
     7621 aatgtcaaga gagccttgct actatcgcca gtgaaaagca aaatctggaa gctgagattg
     7681 aagaaattaa gtctaataaa aacgccatcc aagaacgcta tcaaaatttg caggaagagg
     7741 tggcgcaagc tcgcttgctt aagacagaac tgcaagggca aaaacgttat gaagtagctg
     7801 atattgagcg tttaggcaag gaattggaca atcttaatat cgaacaagaa gaaattcagc
     7861 gcatgctcca agaaaaagtt gacaatcttg agaaggttga tacagaattg ctcagtcaac
     7921 aggcggaaga atccaaaact cagaaaacaa atctccaaca aggtttgatt cgcaagcagt
     7981 ttgagttgga tgatatagaa ggtcaactgg atgatattgc cagtcacttg gatcaagctc
     8041 gccagcagaa tgaggagtgg attcgcaagc aaacacgtgc tgaagccaag aaagaaaagg
     8101 tcagcgagcg cttgcgccat ctacaaaatc aattaacaga ccagtaccag attagctata
     8161 ctgaagcact agaaaaggca catgaattgg aaaacctcaa tctggcagag caagaggtgc
     8221 aggatttaga gaaggctatt cgctcattgg gacctgtcaa cttggaagct attgaccagt
     8281 acgaagaagt tcacaaccgt ctggactttc taaatagtca gcgagatgat attttgtcag
     8341 cgaaaaatct gctccttgaa accattacag agatgaatga tgaggtcaag gaacgcttta
     8401 aatcaacctt tgaagctatt cgtgagtcct ttaaagtgac cttcaagcag atgtttggcg
     8461 gaggtcaggc agacttgata ttgactgagg gcgacctttt aacagctggt gtggagattt
     8521 ctgttcaacc tccaggtaag aaaatccagt cgcttaacct catgagtggt ggtgaaaaag
     8581 ctctatcggc tcttgccttg ctcttctcca ttattcgtgt caagaccatt ccttttgtca
     8641 tcttggatga ggtggaagct gcgctggatg aagccaatgt taaacgtttt ggggattacc
     8701 tcaaccgctt tgacaaggac agccagttca tcgtcgtaac ccaccgtaag ggaaccatgg
     8761 cagcggctga ttccatctat ggagtgacca tgcaagaatc gggtgtttca aagattgttt
     8821 cagttaagtt aaaagattta gaaagtattg aaggatgaca attaaactag tagcaacgga
     8881 tatggacgga accttcctag atgggaatgg acgctttgat atggatcgtc tcaagtctct
     8941 cttggcttcc tacaaggaaa aagggattta ctttgcggtg gcttcgggtc ggggatttct
     9001 gtctctggaa aaattatttg ctgatgttcg tgatgacatt attttcatcg cggaaaatgg
     9061 cagtttggta gagtatcaag gtcaggactt gtatgaagcg actatgtctc gtgactttta
     9121 tctggcaact tttgaaaagc tgaaaacgtc accttatgta gatatcaata aactgctctt
     9181 gacgggtaag aagggttcat atgttctaga tacggttgat gagacctatt tgaaagtgag
     9241 tcagcattat aatgaaaata tccaaaaagt agcgagtttg gaagatatca cagatgacat
     9301 tttcaaattt acaaccaact tcacagaaga aacgctagaa gctggtgaag cttgggttaa
     9361 tgaacatgtc cctggtgtta aggccatgac aactggcttt gaatctattg atattgttct
     9421 ggactatgtc gataagggtg tagctattgt tgaattagct aaaaaacttg gtatcacaat
     9481 ggatcaggtc atggcttttg gagacaatct taatgactta catatgatgc aggttgtggg
     9541 acatcctgta gctcctgaaa atgcacgacc tgaaatttta gaattagcaa agactgtgat
     9601 tggtcaccat aaggaacggt cggttatagc ttatatggag ggcttataat ggcagatata
     9661 aaattgattg cattggactt ggacgggacc ttgctgacta ctgataaaag gctgacggat
     9721 cgtaccaagg aaaccttgaa agctgcgcgt gatcgtggta tcaaggtcgt attgacaact
     9781 ggtcgtccct taaaagccat ggatttcttt ctccatgagt tagggactga cggtcaggaa
     9841 gatgagtata ccattacttt taatggtgga ttagttcaga aaaatacagg agaaatcctt
     9901 gataaaacag tcttttcata tgatgatgtg gcacgtttgt atgaagaaac agagaaatta
     9961 tcactgcctc ttgatgccat ctcagaagga acagtttatc aaatccaatc ggaccaagaa
    10021 agtctttatg ccaaattcaa tccagctttg acctttgttc cagtggactt tgaagactta
    10081 tctagtcaaa tgacctacaa caaatgcgtg actgcctttg ctcaagaacc cttggatgca
    10141 gccattcaga agatttctcc agaattgttt gaccaatatg aaatctttaa atcacgtgaa
    10201 atgttgctag aatggtcacc aaagaatgtt cataaagcaa caggtttggc aaaactaatc
    10261 agccatcttg gaatcgacca aagtcaagtg atggcttgtg gtgacgaggc caatgacctc
    10321 tctatgattg aatgggcagg tcttggtgtt gctatgcaaa acgctgttcc tgaagtaaag
    10381 gcagccgcaa atgtagtgac gccgatgacc aacgatgagg aagctgtcgc ctgggctatc
    10441 gaagaatatg tgctaaagga gaactaagat atgggattgt ttgaccgtct attcggaaaa
    10501 aaagaagaac ctaaaatcga agaagttgta aaagaagctc tggaaaatct tgatttgtct
    10561 gaagatgttg agcctacctt cacagaagtt gaggaagttt ctcaggaaga agcagaggtt
    10621 gaaattgttg aacaagctgt gttccaagaa gaggaaatcc aagacacagt tgaagaaagt
    10681 ctggatttag agccagttgt agaagtttct caaaaagaag tcgaagaatt tccacactca
    10741 gaagaaggga atactgagtt tctagagact atagaagaaa ataattctga agttcttgaa
    10801 ccagaaaggc ctcaagcaga agaaactgtt caggaaaaat atgaccgtag tcttaagaaa
    10861 actcgcacag gtttcggtgc tcgcttgaac gccttctttg ctaatttccg ttctgttgac
    10921 gaagaatttt tcgaggaact ggaagaactg ctgatcatga gtgatgttgg tgtccaagtc
    10981 gcttctaact taaccgagga actacgctac gaagccaagc ttgaaaacgc caagaaaccc
    11041 gacgcactac gccgtgttat cattgagaaa ttggttgagc tttatgaaaa aggtggtaac
    11101 tatgatgaaa gaatccactt ccaagctggc ttgacagtga tgctctttgt tggtgttaat
    11161 ggtgttggga aaacaacttc tatcggaaaa ctagcccacc gctacaaaca agctggcaag
    11221 aaagtcatgc tggttgcggc agataccttc cgtgcaggag cagtagccca gcttgctgaa
    11281 tggggccgtc gtgttgacgt tccagttgta actggacctg aaaaagctga cccagccagt
    11341 gtagtctttg atggaatgga acgtgctgtg gctgaaggta tcgatattct catgattgat
    11401 actgcaggtc gtctacaaaa caaggacaac cttatggctg agctggaaaa gattggtcgt
    11461 attatcaaac gtgttgtacc agaagcacct catgaaacct tcttggctct tgatgcatca
    11521 acaggtcaaa atgccttggt acaggccaag gaattttcaa aaatcactcc tttaacagga
    11581 attgtcttga ctaagattga tggaactgcc cgaggtggtg ttgttctagc catccgtgaa
    11641 gaactcaata ttcctgtaaa attgattggt tttggtgaaa aaatcgatga tattggagag
    11701 tttaactcag aaaactttat gaagggtctc ttagaaggct taatctaatc agaagcaaaa
    11761 atcctgcaag gtacaaactt gcaggaaatt tttttactct aaacgaccat cttgacgata
    11821 ggcgatatct ggttgccaag tccatttggc actgaatttt tcaagtaagt caaagcttgc
    11881 ttgaggtccc atacttccag ctttatagtc atgaagtggg gcaccatttt cagcccatag
    11941 ctcttcaata cggtcaatca atttccatga cgcaccaact tcatcccagt ggctaaagtt
    12001 agttgagttg ttatttaaga catcataaat caatttctcg tatggttctg gagaagcacc
    12061 agttgcggtt gcatctgtac ggtaatcaag tgagttagga gccaagttga attcttctcc
    12121 tacttgcttc ccatttaggc taagagagaa gccttctgtt ggttggatat agatggtcaa
    12181 aatatttgga gcaagtggtt ctccaaagat agagtccatt tgtttaaaga cgatgttgac
    12241 atgagttcct ttttcagtca gacgtttacc agtacggaag aagaaaggaa cactacggaa
    12301 gcgatcgctg tccacaaaga aggcaccaga tgcaaaggtt tcagttgttg attctggatt
    12361 tacatttggc tcgctacggt aagagatgta tttcatgcca tcaatcttac ctgaacggta
    12421 ttgaccacgg ataaagtgtt ctttgagctc ttcatcagtt ggatgataga ggtttttaaa
    12481 gaccttaatc ttttcagcac gaatctcgtc ctttgtgaag ctggctggtt tgtccatagc
    12541 aaggagagaa agaagttgca aagtgtggtt ctgtaccatg tcacggaggg caccagattc
    12601 atcatagtag ccaccacgtt cttctacgcc taagcgttcc gcaaaggtaa tttgaacatt
    12661 gtcgataaaa tccttgttcc aaacgttttc aaaaatcaag tttgcaaagc gaactgcaaa
    12721 gatactttgg atcatttcct taccaagata atggtcaata cggaaaattt gttcttcgtc
    12781 aaatgttgct aggagttcat cattcaactt gcttgcgctt gcgtaatctg taccaaatgg
    12841 tttttcaacg attaaacgtt caaaaccttt tccatctacg atattttcag acttgaggtg
    12901 cttagcaatg gttccaaaga attgaggcgc catagacaag aagaagagct tgttgtgttc
    12961 agcttggtac ttgtcattta gctcagtctg caattgacgt aaagcaatgt agtgctctgt
    13021 atcattgaca tcatgacttt gatagtagaa gtggctagcg aactcttgag cctgttcggt
    13081 actatctgcc aaatcaagga tagattcgac tacaacagat tcaaaatatt ccttgctcca
    13141 aggacgacgg gcagttccaa taacggcaaa atgtttggaa agattgccgg atttatatag
    13201 tctaaaaagg gaagggtaga gcttgcgttt agccaggtct ccactcgcac cgaaaattgt
    13261 aacaataacc ttagatgaca tctagctacc taatttctat ttttattcct agtcttgcta
    13321 ggtatgagga aacctcattt cataaactta attctaacat aattttccga aaaatcaaaa
    13381 attccaatac gaggtagaaa aactgcaagg aaatccttac agtttgagtt tagacgttta
    13441 agaccttatc taagaactct ttcagacgag ggtgttgtgg gttatcaaag atttggtcag
    13501 gtgttccgtc ttcaaggaac tcgccatctg cagtaaagat aacgcggttg gcaacctgac
    13561 gagcaaatcc catctcatgg gttacgataa tcatggtcat gccttgctca gccaattcct
    13621 tcataacgtt aagtacgtct ccaaccatct cagggtcaag ggcagaagtt ggttcatcga
    13681 agagcatgat gtctggattc attgctaggc cacgagcgat ggccacacgt tgtttttgac
    13741 cacctgatag gctatctgga ttggcattag ctttatctgc tagtccaacc ttttcaagca
    13801 actccattcc caattcctca gcttcttcct tagtcatcaa cttgtgctca ataggagcaa
    13861 aggtgatgtt gtccaataca gacatatgag ggaagaggtt gaagtgttgg aataccatgc
    13921 cgatattttc acggacgtgg tcaacattgg ttgttttttc agttaaatca tagccgttca
    13981 cagtgatgtg accgctagtg acttcttcta aaagattgag gctacggagg aaagttgact
    14041 taccagaacc tgaaggaccg atgatacaaa caacatctcc ttcatagaac ttagtcgtaa
    14101 ttccttttag gacttcattt tttccatagt gcttgtgtaa atcatttaca tcaattttta
    14161 attttgccat taacgaatcc tcttttctaa gcgtttcgct agtctagtca aaagtgtgat
    14221 aattacaaga tagaagatag caaggattgc atacatcttg aaactttggt agttacgagc
    14281 aatgataatc ttaccagttt ggaagagttc aaccaaaccg atagcagata cgatagttgt
    14341 atctttaaga gcgataacga attggttgac aaagtttggc aacatcaatt tagttgcttg
    14401 tggcaagata atcttacgca tggtttttcc ataagagata cccaagcttc ggctggcttc
    14461 catttggcca actggaacgg cctgaatacc accacgaacg atttcagcga tataagcagc
    14521 cgcattgagt gagagggcaa tggttccagc tacaaagtcg ttaattgggc tttgttggcc
    14581 tgtgatagac tcgatgaagt ttggaattcc ccagaagatg aaggctgcaa gaatcatcaa
    14641 tggaatacca cgaataacgt caacgaaaat ctcagagatg acgcgaagag atttgtatgg
    14701 gctaacgcta aacataccga agataattcc gatgacaatg gcaatagcaa atgagataag
    14761 agctagagca agagtgatac caagaccgct aaggagttgt ttgtagttgt tttgaagcaa
    14821 gccccagagc gttgtttcgt caacagtact tgttgaagca gttgaagatt cgctagctag
    14881 gtatttgtca agaatctttt ggaattcacc gtttgcttta aggtttgcaa gtccgttgtt
    14941 gaacatttca atcagttctg gatttgctcc ttttttaacg gcaaaggctg tttcaccgat
    15001 tggagttcca gagattggag ttttcaattt ttgaccttgg ctgatagaat atttgagaac
    15061 aggttcatca tccataacgg catcaatggc accagtgttt aaactgtcat acattgaaga
    15121 accatcagca aaggttttga ttttgtaacc gtatttgctt tgattttctg ttaggaaggt
    15181 ttgagaagca gttccgtttt taacaccgac tgtctttcct tttagatctt cataagaagc
    15241 aatattgctt gattctttga caccaagaat ggtattagca gtgtagtatg attctgagaa
    15301 gtcaaaagtt gccttacgag catctgtgac agacatacca gcaatgatac catcggcttg
    15361 accagcttgg acagcactga tagcagcatc aaaaccaggg ttggtgattt caatttcaaa
    15421 accttggtct ttagcgattg ccttaatcaa ttccatatca ataccagtgt attggttgct
    15481 tgaattttgg aaaacaaaag gtgcaaaaga agaatcgctg gcaatgatat atttagcctt
    15541 aacaggaata gcttttaatc ctgcgagagt agttgtagtt ggcactgctg aagatgatga
    15601 agcagtccat ttcttgataa ttttatcaag actaccatct tttttcattt cagccaaggc
    15661 ttggttaaat tcagtaacca ggtgttcgta tttacttcct tttttcacac cgaaagcaaa
    15721 acttcctaca gcttcaccat ccatttcaat atggaggtct tgaccttggt taatggcata
    15781 ttcgataaca ggtttgtcat ccatcatggc atcgatggca ccagcactca agctgttgtt
    15841 cattaaatcc ccagtgtcaa atgttttaat agtaaagccg tatttatctt tgattgtttc
    15901 aaggaaacgt tgagcggcag ttccgttttt aacaccaacg gttttgccag ttaattggtc
    15961 gtacttgcta attttgtgtg actttgtagt agcaatgaca acttttgtat catagtaagt
    16021 atcagacatg gtgaagactt tttcacgttc tttagtcttt gtcatccctg ccatgatagc
    16081 gtcggcttgc ccagcttgaa ccgcattgac tgctgcgtca aatccaggat aggacatctg
    16141 aatgttccag cctttaatct cagcgacttt gttaataatg tcaacatcaa ttcctttata
    16201 agtttgatct gaatctttaa actcaaaagg tgcataggcg gtatcagaaa caatcttaat
    16261 cgtttctgct ttggcaatac ctaatgagaa agttgggaat aaaattagca aaaatgctag
    16321 aaattttttc ttcattttta gtctcctttt ccgaatattc tctcattata tcagaaaaag
    16381 taaaaaaagg caaattttta tatttttcgt gtcagaaaat ataacattga tttttccttt
    16441 tctttcttga attgctat
//
LOCUS       DAVKJD010000038        16171 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKJD010000038 DAVKJD010000000
VERSION     DAVKJD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16171
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.038"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            97..318
                     /locus_tag="VW157_001254"
     CDS             97..318
                     /locus_tag="VW157_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9552553.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTDSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            835..1074
                     /locus_tag="VW157_001255"
                     /pseudo
     CDS             835..1074
                     /locus_tag="VW157_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1218..2447
                     /locus_tag="VW157_001256"
     CDS             1218..2447
                     /locus_tag="VW157_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9552554.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2467..5163
                     /gene="ppc"
                     /locus_tag="VW157_001257"
     CDS             2467..5163
                     /gene="ppc"
                     /locus_tag="VW157_001257"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9552555.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDVYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5246..5463
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5576..6580
                     /locus_tag="VW157_001258"
     CDS             5576..6580
                     /locus_tag="VW157_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9552556.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEVQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSIGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6649..7515
                     /locus_tag="VW157_001259"
     CDS             6649..7515
                     /locus_tag="VW157_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552557.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7512..8270
                     /locus_tag="VW157_001260"
     CDS             7512..8270
                     /locus_tag="VW157_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552558.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRVIQDLNQEEKAKMKISDYYQLFE"
     gene            8360..10120
                     /gene="dnaG"
                     /locus_tag="VW157_001261"
     CDS             8360..10120
                     /gene="dnaG"
                     /locus_tag="VW157_001261"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9552559.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTVIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENGENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEVHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10123..11232
                     /gene="rpoD"
                     /locus_tag="VW157_001262"
     CDS             10123..11232
                     /gene="rpoD"
                     /locus_tag="VW157_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9552560.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11246..11575
                     /locus_tag="VW157_001263"
     CDS             11246..11575
                     /locus_tag="VW157_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9552561.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11690..12733
                     /gene="cpoA"
                     /locus_tag="VW157_001264"
     CDS             11690..12733
                     /gene="cpoA"
                     /locus_tag="VW157_001264"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9552562.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12749..14074
                     /locus_tag="VW157_001265"
     CDS             12749..14074
                     /locus_tag="VW157_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9552563.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14105..14281
                     /locus_tag="VW157_001266"
     CDS             14105..14281
                     /locus_tag="VW157_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9552564.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14340..14474
                     /locus_tag="VW157_001267"
     CDS             14340..14474
                     /locus_tag="VW157_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9552565.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14538..15848
                     /gene="obgE"
                     /locus_tag="VW157_001268"
     CDS             14538..15848
                     /gene="obgE"
                     /locus_tag="VW157_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9552566.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVTNKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEVYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15858..16016
                     /locus_tag="VW157_001269"
     CDS             15858..16016
                     /locus_tag="VW157_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552567.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         4964 a         2824 c         3621 g         4762 t
ORIGIN      
        1 tgtttaataa attaatttta gttcaaaatg tccagtttgg acactttgaa tgatttctta
       61 gttttatata ttatactttt ctcaggagaa agctagatgt acagacgttt gagagatttg
      121 agggaagatc acgatttgac ccaaaaacaa atagctaaaa tactttcgtt tacagactca
      181 gcttatgcta aaattgaacg gggtgagcat acgttaacag cagatgtatt ggtaactctc
      241 tcaaactttt acgatgtcag tacagactac ctattggaat tgacagattt tcctgataaa
      301 attcgcttta gaaaataatc tcctcaattt catagagtgt gaaaatgagg agatttttta
      361 tttgttcttt gaaaattaaa tagtccaaaa tggtactttc ctcatttgtg gagcaaattt
      421 gaatggctcg ccatgataag agcgatttta aaaccatcaa taaaatagag cgatacttta
      481 tatgccatga tacaaatgat atacaatgat acttctgacc gttcaggctg ccatcgtaaa
      541 agagcagcaa gtgaaattct tatgatgact tcatcagtca tgccacgttg aatgtgtgag
      601 tttgttagag aaacgcaatt aatcctcaaa aggttccccg aaccttttga gttccacaga
      661 cgcatcacgt ggagtgtgta agcttgttgc taaaagcgta aaaaccttgg aacgaaagga
      721 ataatagact tcctgcgaaa caaaaataca atacaataaa actatggatg atgaagcaag
      781 taaacaatta agcgatagcc gtttcaagag ccttgtaggt gttcagcgca cgagatgtta
      841 gctgtgttaa aaacagctta tcaacgcaac gtaaacgcgc aaaaggtgga cgaaaaacca
      901 aattaagtct agacgatctc ctcatggtaa ctattcaata catgcgagaa tagcgcactt
      961 atgaacaaat tgcggctgat tttgacattc acgaaagcaa cataattagt cggaggtcaa
     1021 tggattgaag caactcttat tcaaaatggt tttacgattt caaattctgc cttaattctg
     1081 taaaaacagt aaaattcgaa ggattgtaag gtaagagttt ttttctttct gaaaaaatgg
     1141 tataatagca atcaaaacta gaaaataaaa cggaatttgg aacagatttg tctgtatcct
     1201 agtagagtgg tgatactatg aagattagta agaggcactt attaaattat tccatcttga
     1261 ttccctactt gcttttatct attttgggct tgattgtggt ctattcgacc accagtgcta
     1321 ttttaattga agaaggcaag agcgccttgc agttggttcg aaaccaagga atcttttgga
     1381 ttgttagttt gatactgatt gccttaattt ataaattgag actagatttt ttgagaaatg
     1441 agcgactaat cattttagtt atattaatag aaatgctttt attgttcttg gctcgtttta
     1501 ttggtatttc agtaaacggg gcatacggtt ggatttcggt tgcaggagta actattcagc
     1561 cagctgagta cttaaaaatc attattattt ggtatttagc tcaccgattc tccaaacagc
     1621 aagaagaaat agctacttat gattttcaag ttttgactca aaatcaatgg cttccccgtg
     1681 cttttaatga ttggcgattc gttctcctag ttctgattgg aagtttggga attttccctg
     1741 atttaggaaa tgcgactatt ttagtcttgg tttccttgat tatgtataca gttagtggaa
     1801 tcgcttatcg ctggttttca accattctgg cgctcgtatc tgccgcttct gtctttgtct
     1861 tgaccactat cagcctaatc ggtgttgaga ccttttcaaa aattccagta tttggctatg
     1921 tagccaagcg ctttagtgcc ttttttaatc cttttgccga tcgtgctgat gcaggtcacc
     1981 agttagctaa ttcttatttt gccatggtca atggtggttg gtttggtcta ggtcttggaa
     2041 actcgattga aaaacgaggt tatttgccag aagctcatac agactttgtc ttttctatcg
     2101 tgattgaaga atttggcttt gttggtgcca gtcttatttt agctctcttg tttttcatga
     2161 ttttgcggat tatcttggtc ggtattcgag cggagaatcc tttcaatgcc atggttgcac
     2221 tcggtgtcgg agggatgatg ttggttcagg tatttgtcaa tatcggaggg atttcgggct
     2281 tgattccatc tacaggagta accttcccct tcttatccca gggtggaaat agtcttctag
     2341 tcttatcagt ggcagtagcc tttgtcttaa atattgatgc cagtgaaaaa cgcgctaagt
     2401 tgtaccgaga attggaaaat caaccaatga accttctgtt gaagtaggat aaagaaagga
     2461 tagtttatgt ctcttcaaaa attagaaaat tctagtaata aaagtgttgt gcaagaagaa
     2521 gtcttgattc taacagaatt actggaagat attactaaaa atatgcttgc cccagagacc
     2581 tttgaaaaaa taatacagtt gaaagaatta tcaacgcagg aagattatca aggtctaaac
     2641 cgtctagtga ctagcttatc aaatgatgaa atggtctata tttcacgcta tttctctatc
     2701 ttgcctcttt tgattaatat ttcagaggat gtggatttag cttatgaaat caatcatcaa
     2761 aataatattg atcaggacta tttaggtaaa ttatctacaa cgattaaatt ggtagcagaa
     2821 aaggaaaatg ccgttgagat cctagaacac ttgaatgttg ttccggtttt gacagcccat
     2881 ccaacacaag tgcaacgcaa aagtatgttg gatttaacaa atcatattca tagtcttttg
     2941 cgtaaatacc gtgatgttaa gttggggttg atcaataaag ataaatggta caatgatttg
     3001 cgtcgttaca tcgaaattat catgcagaca gacatgattc gtgagaaaaa attaaaagtg
     3061 actaacgaaa tcacgaatgc tatggaatat tataacagct cctttttgaa agctgtacct
     3121 catttgacga cggagtataa gcgcttagcg caagcgcatg gtctgaattt aaaacaggct
     3181 aaaccaatca ccatgggtat gtggataggt ggtgaccgtg atggaaatcc atttgttaca
     3241 gcaaagacct tgaagcagtc tgcactcact cagtgtgaag tcatcatgaa ctactatgat
     3301 aaaaagattt accaacttta tcgtgaattt tctctttcaa ctagcattgt caacgtcagc
     3361 aagcaagtca gagaaatggc tcgtcaatcc aaggataact cgatttaccg cgaaaaagag
     3421 ctttaccgtc gtgccttgtt tgatattcaa tcaaaaattc aggcaactaa aacctatctg
     3481 attgaggatg aagaagttgg gactcgttat gaaaccgcca atgatttcta caaggatttg
     3541 attgccattc gagattctct actagaaaat aagggcgagt ccttgatttc aggtgatttt
     3601 gtggaattat tgcaggcagt agagatattt ggtttttact tagcatcaat tgatatgcga
     3661 caagactcta gcgtctatga agcctgtgtg gcagaactct tgaaatcagc aggaattcat
     3721 tctcgttata gcgagttgag cgaagaagaa aagtgtgacc ttctcttgaa agaattagaa
     3781 gaagatcccc gaattctttc tgcgactcac gcagaaaaat cagaattatt agcaaaagaa
     3841 ctagctattt ttaagacggc tcgtgttttg aaagataagt tgggagatga tgtcatccgt
     3901 cagaccatca tttcacatgc aaccagcctt tctgatatgc tagaattagc tattctgtta
     3961 aaagaagtag gactggtgga tacggaaagg gcgcgtgttc agattgttcc cctttttgaa
     4021 acaattgaag acttggatca ttcagaggaa acaatgagaa aatatctttc tcttagcctt
     4081 gccaaaaaat ggattgactc acgaaataac taccaagaaa tcatgcttgg ctactctgac
     4141 agtaataaag atggcggtta cttgtcatca tgttggaccc tctacaaggc tcaacaacaa
     4201 ttgactgcta ttggagatga atttggcgtt aaggttacct tcttccatgg tcgtggtggt
     4261 actgtcggtc gtggtggtgg gccaacctat gaagccatta catctcaacc gctcaagtct
     4321 atcaaggatc gtatccgctt gacggagcag ggtgaagtaa ttgggaataa atacggtaac
     4381 aaagacgccg cttactataa ccttgaaatg ctagtatcgg cagctattaa ccgtatgatt
     4441 actcagaaga agagcgatac caatacccca aatcgttatg aagccattat ggatcaagta
     4501 gtggaccgta gttacgacgt ctaccgtgat ttggtctttg gtaatgagca tttctatgat
     4561 tatttcttcg agtcaagtcc aatcaaggct atttcaagtt ttaatattgg ttctcgtcca
     4621 gccgctcgta agactattac tgaaatcggt ggattgcgtg ccatcccttg ggtattctca
     4681 tggtcacaga gtcgtgttat gttccctgga tggtacgggg ttggttcaag cttcaaggaa
     4741 tttatcaata aaaatccaga gaatattgct atcttacgag atatgtacca aaattggcct
     4801 ttcttccaat cgcttctttc aaatgttgat atggttttgt caaaatcaaa tatgaatatt
     4861 gcttttgaat atgctaaact ttgtgaagac gagcaagtta aggccatcta tgagactatt
     4921 ttaaatgaat ggcaagttac taagaacgtt atcttggcta ttgaaggaca tgacgaactc
     4981 ttagctgaca atccatatct aaaagctagt ctggattacc gtatgcctta ctttaatatt
     5041 ctcaactata ttcagttgga gttgattaaa cgccaacgtc gtggagaatt gtccagtgat
     5101 caagaacgat tgattcatat caccatcaac ggaattgcga caggattgcg taattcaggt
     5161 tgataatttt caagagtgaa tgctaaaagt gaatatcaaa aaaattctaa tagactattg
     5221 acaagtagtt taaaaatgat ataatttaac cattcagaaa agtaatcata caaacttttt
     5281 agagagtctg tggtagctga aaacagataa gtggcgatga tgaaaattgg gctgaatgct
     5341 atttagaatt tgaaattata aaaattcggt aagcacacct tacagtgcat ctcgttattg
     5401 cgagactgag cgatagggaa attccctata attgaggtgg taccgcgcat cgacgtcctc
     5461 acacaagttt tttgtgtgag gatttttttg atggaggtta gtatggaaag aaaacgatgg
     5521 cgtcgcttgt ttagataagt gaaatatgtt aaaggaaata aaaaggagaa acagaatgaa
     5581 aaataaacgt ttaattggaa ttattgctgc attagcagtc ttagtagcag gaagcttgat
     5641 ttattcttca atgaataaat cagaagttca gaataataag gatgagaaga aaataaccaa
     5701 gattggtgtg cttcaatttg tgagccatcc atcccttgat ttgatttata aagggatcca
     5761 agatggactt gcagaagaag gatataaaga tgatcaagtt aaaattgatt ttatgaactc
     5821 agaaggtgac caaagtaagg ttgcgacaat gagtaaacaa ttggttgcaa atggaaatga
     5881 ccttgtggtt ggtatcgcaa caccagcagc ccaagggttg gctagtgcaa caaaagacct
     5941 accggttatc atggccgcta ttacagaccc aattggtgct aacttggtta aagatttgaa
     6001 aaaaccaggt ggcaacgtta caggggtatc tgaccacaat ccagctcaac aacaagttga
     6061 actcatcaag gctctgacac cgaatgtgaa aacaatcgga gctctttact caagtagcga
     6121 agacaattca aaaacacagg tcgaagaatt taaggcttat gctgaaaaag caggtctgac
     6181 agtggaaaca tttgcagttc cttcaacaaa tgaaattgcc tcaactgtca ctgttatgac
     6241 tagcaaggta gatgctattt gggttccaat tgataacacc attgcatcag gatttccaac
     6301 ggttgtctct agcaatcaaa gttctaagaa accaatttat cccagtgcga cagctatggt
     6361 agaagtaggt ggtttggcat cagttgtaat tgaccaacat gaccttggtg tggcaacagg
     6421 taaaatgatt gtgcaagtct tgaaaggtgc aaaaccagcc gataccccag tcaatgtctt
     6481 ttcaattggt aagtcagtca tcaataaaaa aatagcacaa gaactaggta ttactattcc
     6541 tgagtctgtt ctcaaagaag caggacaagt catcgaataa ataaggtagg gaggaggaga
     6601 ctcttcccat tttttaaaaa gatgaatata gaaaggatta aataaaatat gattgtttcc
     6661 attatttctc aaggatttgt ctgggctatt ctaggtctgg gaatctttat gacatttagg
     6721 attttaaact ttccagatat gacgacagaa ggttccttcc ctcttggggg agctgttgct
     6781 gtcactttga taaccaaagg cgtgaaccca tttttagcga cacttgttgc tgtaggagca
     6841 ggttgtttgg ctggaatggc agcaggcctt ctttatacaa aagggaagat cccaaccttg
     6901 ctctcaggga ttttggtgat gacttcttgt cactcaatca tgctcttgat tatgggacgt
     6961 gcgaatttag gcctgcttgg aaccaagcaa attcaggatg ttttgccttt tgattcggat
     7021 ttgaatcaac tcttgacagg tctcatcttt gtgagtattg ttattgctct catgctcttt
     7081 ttcttggaca ctaaactcgg acaagcctat attgctacag gggataatcc tgatatggct
     7141 agaagtttcg ggattcatac tggacgcatg gagctcatgg gcttggtctt atcaaatggt
     7201 gtgattgccc ttgcaggtgc cctcattgct cagcaagaag gttatgccga tgtgtctcga
     7261 gggatcgggg ttatcgttgt ggggcttgca agcttgatta ttggagaagt tattttcaag
     7321 agtttgagct tggcagagcg tttggttact atcgttgtag gttctatcgc ttatcaattt
     7381 ttagtgtggg cagttatcgc acttggcttt aatacaagtt accttcgttt atacagtgcc
     7441 ttgattttag cagtctgcct catgattcca acatttaagc aaacaatctt gaaaggagcc
     7501 aagttaagca aatgacagca attgtagaat taaaaaatgc aaccaaaatc gttaaaaatg
     7561 gctttgatga agaaaagatt attttaaatg atgtttcctt agaaattttt gaacaggact
     7621 ttatcacgat tttgggtgga aatggtgctg gtaaatcaac cctctttaac actattgcag
     7681 gaaccatatc actaaccagt ggaactatcc gtattttagg tgaggatgtt accaagtttt
     7741 caccagagaa gcgcgctaag tatctgtctc gagtcttcca agatccaaag atggggacag
     7801 ctcctcgtat gacagtggca gagaatctcc tgattgccaa gtttcgtggt gaaaagcgtg
     7861 gactatttcc gagacgtctg gctagctata aggataagtt tcaggcgacc attgaaaaag
     7921 tgggaaacgg tcttgagaaa cacttgaata caccgattga gttcttatca ggtggacaaa
     7981 gacaggcttt gagtctcttg atggcaacct tgaagcgacc tgaattactc ttgttagacg
     8041 agcatactgc tgccctggat ccaaagacta gtgttgcttt gatggaattg acagatgaat
     8101 ttgttaagaa agatcaattg acagccctta tgattaccca tcatatggaa gatgctctca
     8161 aatatggcaa tcgcttgatt gtcatgaaag aaggacgggt tattcaagat ttaaaccaag
     8221 aagaaaaagc aaaaatgaaa atctctgatt attatcaact ctttgaataa agtagggaaa
     8281 atgagtgaaa tggtttactt tttttctgaa ataaagtata ctatataaag taaactatga
     8341 taacatggag gtattgtgta tggttgacaa acaagtcatt gaagaaatca aaaacaatgc
     8401 caacattgtg gaagtcatag gagatgtgat ttctttacaa aaggcaggac ggaactatct
     8461 agggctctgt ccttttcatg gtgaaaaaac accttctttc aacgttgtag aggataagca
     8521 gttttatcac tgtttcggtt gtggtcgctc aggtgatgtt tttaagttca tcgaggagta
     8581 ccaaggggtt ccctttatag aggcagtcca aatcttaggt cagcgtgttg gaattgaggt
     8641 tgaaaaaccg ctttatagtg agcagaagcc agcctcacct caccaagctc tttatgatat
     8701 gcacgaagat gcagccaaat tttaccatgc gattctcatg acaacgacta tgggcgaaga
     8761 ggccagaaat tacctttatc agcgggggtt gacagatgaa gtgcttaaac atttttggat
     8821 tggtttagca cctccagaac gaaactatct ctatcaacgt ttgtctgatc agtatcgtga
     8881 agaggattta ctggattcag gcctgtttta tctctcggat gccaatcaat ttgtagacac
     8941 ctttcacaat cgcattatgt ttcccctgac aaatgaccaa ggaaaggtca ttgccttctc
     9001 aggtcgtatc tggcaaaaaa cggattcaca aacttctaag tataaaaaca gccgatcgac
     9061 tgtaattttt aacaaaagtt acgaattata tcatatggat agggcaaaaa gatcttctgg
     9121 aaaagctagt gagatttacc tgatggaagg attcatggat gttattgcag cctatcgggc
     9181 tggaatcgaa aatgctgtgg cgtcgatggg aacggccttg agtcgagagc atgttgagca
     9241 tctgaaaagg ttaaccaaga aattggttct tgtttacgat ggagataagg ctgggcaagc
     9301 cgcgacattg aaagcattgg atgaaattgg tgatatgcct gtgcaaatcg tcagcatgcc
     9361 tgataacttg gatcctgatg aatatctaca aaaaaatggt ccagaagact tggcctatct
     9421 attaacgaaa actcgtatta gtccgattga gttctacatt catcagtaca aacctgaaaa
     9481 cggtgaaaat ctgcaggctc agattgagtt tcttgaaaaa atagctccct tgattgttca
     9541 agaaaagtcc atcgctgctc aaaacagcta tattcatatt ttagctgaca gtctggcgtc
     9601 ctttgattat acccagattg agcagattgt taatgagagt cgtcaggtgc aaaggcagaa
     9661 tcgcatggaa agaatttcca gaccgacgcc aatcaccatg cctgtcacca agcagttatc
     9721 ggctattatg agggcagaag tccatctact ctatcggatg atggaatccc ctcttgtttt
     9781 gaacgattac cgtttgcgag aagactttgc atttgctaca cctgaatttc aggtcttata
     9841 tgacttgctt ggccagtatg gaaatcttcc tccagaagtt ttagcagagc agacagagga
     9901 agttgaaaga gcttggtacc aagttttagc tcaggatttg cctgctgaga tatcgccgca
     9961 ggaacttagt gaagtagaga tgactcgaaa caaggctctc ttgaatcagg acaatatgag
    10021 aatcaaaaag aaggtgcagg aagctagcca tgtaggagat acagatacag ccctagaaga
    10081 attggaacgt ttaatttccc aaaagagaag aatggagtaa taatggcaac aaaacaaaaa
    10141 gaagtaacaa catttgacgt acaggtagca gaatttatcc gtaatcataa gcaaaaaggg
    10201 acagcaacag atgatgaaat caatgcaagt ctggttattc cttttacctt ggacgctgat
    10261 gggattgaag atctcttgca acggattcag gatgcaggga tttctatcac agataacgaa
    10321 ggaaatccaa gtgcgcgtgt tcttagcaat gaagaagaac cagaactcag cgatgaggac
    10381 ttgattggat caacttctgc taaggtcaat gaccctgtcc gtatgtactt gaaagaaata
    10441 ggggtcgttc ctctcttgac caatgaagag gagaaagagt tggcactggc tgttgaagct
    10501 ggcgatatcg aagccaaaca acgtcttgcg gaagccaatc ttcgtttggt tgtttccatt
    10561 gccaaacgct atgtcggtcg tggcatgcag ttccttgact tgattcaaga aggaaatatg
    10621 ggcttgatga aggcggttga caagtttgac tattctaaag ggttcaagtt ttcaacttat
    10681 gcaacttggt ggattcgtca ggctatcact cgtgctattg cggaccaagc tcgtaccatc
    10741 cgtatcccag ttcacatggt tgaaactatc aataaattgg ttcgtgaaca gcggaatctc
    10801 cttcaagaat tggggcaaga tccgacacca gaacagattg ctgaacgaat ggatatgaca
    10861 cctgataagg ttcgtgaaat cttgaagatt gcccaagaac cagtatctct tgaaactcct
    10921 atcggtgaag aggacgatag ccaccttgga gactttatcg aagatgaagt gattgaaaat
    10981 ccagtggatt atacgactcg tatcgtcttg cgtgagcaat tggatgaaat cttagatact
    11041 cttacagacc gtgaagaaaa tgttctgcgt ctacgttttg gactagatga tggaaaaatg
    11101 cgcacacttg aagatgtggg gaaagtcttt aacgtaactc gtgagcgtat ccgtcagatt
    11161 gaagcaaagg ctttgagaaa actacgccaa ccaagccgca gcaaaccgct tcgtgatttt
    11221 attgaagact aagagtgagg aaaatatggc ttatacagaa gagcaaattg aaaacattaa
    11281 aacacggatt ttaacagcct tggaagaagt catcgaccct gagttgggaa tcgatattgt
    11341 caatcttggt ttgatctatg agattcgttt tgacggtgac acagggcaaa cagagattga
    11401 catgactttg acaactatgg gttgtccctt agcagacctt ttgacagacc agatttatga
    11461 tgctatgata gaggttccag aagtaacgga tactgaggtt aaattagtct ggtatccagc
    11521 ctggacagtt gaaaaaatga gtcgctatgc ccgcattgcc ctaggcatta agtaaacata
    11581 taaaagcatg tgagagactg ttggaaaagc gaggaaattt cccctctttt cctctagtct
    11641 ctcctttctt ttgctgattt tattcaaaga aaatgatata atagtagtta tggagaaaaa
    11701 gaaattacgc atcaatatgt tgagttcaag tgagaaagta gcaggacagg gagtttcagg
    11761 tgcttaccgt gaattagttc gtcttcttca ccgtgctgcc aaggaccaat tgattgttac
    11821 agaaaatctt ccgatcgagg cagatgtgac tcactttcat acgattgatt ttccctatta
    11881 tttatcaacc ttccaaaaga aacgctcagg gagaaagatt ggctatgtgc atttcttgcc
    11941 agctacactt gagggaagtt tgaaaattcc atttttctta aagggaattg tgaaacgcta
    12001 tgtattttct ttttacaacc ggatggagca cttggttgtg gtcaatccta tgtttattga
    12061 ggatttggta gcagctggta ttccacgtga aaaagtgacc tatattccta actttgtcaa
    12121 caaggaaaaa tggcatcctc taccacaaga agaggtagtc agactgcgca cagatcttgg
    12181 tcttagtgac aatcagttta tcgtagtagg tgctgggcaa gttcagaaac gtaaagggat
    12241 tgatgacttt atccgtctgg ctgaggaatt gcctcagatt acctttatct gggctggtgg
    12301 cttctctttt ggtggtatga cagatggtta tgaacactat aagaaaatta tggaaaatcc
    12361 ccctaaaaat ttgatttttc caggcattgt atcgccagag cggatgcgcg aattgtatgc
    12421 tctagcggat cttttcttgt tgcctagtta caatgagctc tttcctatga ctattttaga
    12481 agctgcgagt tgtgaggctc ctattatgtt gcgtgattta gatctctata aggtgatttt
    12541 ggagggaaat tatcgggcga cagcgggtag agaagagatg aaagaggcta ttttggaata
    12601 tcaagcaaat cctgctgcct taaaagatct caaagaaaag gctaagaata tttccagaga
    12661 gtattctgaa gagcatctgt tacaaatctg gttggacttt tatgagaaac aagccgcttt
    12721 agggagaaag taaaaagtga ggtaatctat gcgaattggt ttatttacag atacctattt
    12781 tcctcaggtt tctggtgttg cgaccagtat tcgaaccttg aaaacagaac ttgaaaagca
    12841 gggacatgct gtttttatct ttacgacgac agataaggat gtcaatcgct acgaagattg
    12901 gcaaattatc cgcattccaa gtgttccttt ctttgctttt aaggatcgtc gctttgccta
    12961 ccgaggtttt agcaaggcac ttgaaattgc taaacagtat cagctagata ttatccatac
    13021 tcagacagaa ttttctcttg gcctgttggg gatttggatt gcgcgtgaat tgaaaattcc
    13081 agtcatccat acctatcaca cccagtatga agactatgtc cattatattg ctaaggggat
    13141 gttgatccga ccgagtatgg tcaagtatct ggttagaggt ttcctgcatg atgtggatgg
    13201 ggttatttgc cctagtgaga ttgtccgtga cttgctatct gattataagg tcaaggttga
    13261 aaaacgggtc attcctactg ggattgaatt agccaagttt gagcgtccgg aaatcaagca
    13321 ggaaaatttg aaagaactgc gtagtaaact agggattcaa gatggtgaaa agacgttgct
    13381 tagtctttcg agaatctcct atgaaaaaaa tattcaagca gttttagcag cctttgctga
    13441 tgttctgaaa gaggaagaca aggttaaact ggtagtagct ggggatggcc cttatctgaa
    13501 tgacctcaaa gagcaagccc agaacctaga gattcaagac tcagtcatct ttacagggat
    13561 gattgctcct agtgagacgg ctctttacta taaagcggcg gatttcttca tttcggcatc
    13621 gacaagcgaa acgcaaggtt tgacctactt ggaaagctta gccagtggaa cacctgtcat
    13681 tgctcacgga aatccttatt tgaacaacct catcagtgat aaaatgtttg gaaccttgta
    13741 ctatggagaa catgatttgg ctggtgctat tttggaagcc ctgattgcaa caccagacat
    13801 gaacgagcat accttatcag agaaattgta tgagatttca gctgagaact ttgggaaacg
    13861 agtgcatgag ttttatctgg atgccattat ttcaaataac ttccagaaag atttggctaa
    13921 agatgatacg gtcagtcagc gtatctttaa gacagttttg tatcttcagc aacaggtggt
    13981 tgctgtacct gtaaaaggat ctagacgcat gttgaaggct tcaaaaacac agttgatcag
    14041 tatgagagac tattggaaag accatgaaga atagaaagag gaacagctat gaaaaaaaca
    14101 attaatgaga agcggtcgtg ataaaaagat tgcgggtgtt tgtgctgggg tggcccatta
    14161 tctggatatg gatccgacta tcgttcaagt catttggggt gttcttactt gctgttacgg
    14221 agctggaatt gtagcttaca ttattttatg gattatcgca ccagtagcaa ctgactattg
    14281 aaaactagct caattcatgc taagataata gaaaatagaa tgtaacgaag gagagaaaaa
    14341 tggcatttgg agataatgga aatcgtaaaa aaactatgtt tgagaaaata accttgttta
    14401 tcgtgattat catgctagta gcaagtttat tgggaatttt tgcaactgca attggtgccc
    14461 tcagtaatct ataaaataga ttcaagaaaa tttagtgact gggatttccc agcccttttt
    14521 taaagtgaga agaaataatg agtatgtttt tagatacagc taagattaag gtcaaggctg
    14581 gtaatggtgg cgatggtatg gttgcctttc gtcgtgaaaa atatgtccct aatggaggcc
    14641 cttggggtgg tgatggtggt cgtggaggca atgtggtctt cgttgtagac gaaggactac
    14701 gtaccttgat ggatttccgc tacaatcgtc atttcaaggc tgattctggt gaaaaaggga
    14761 tgaccaaagg gatgcatggt cgtggtgctg aggaccttag agttcgagta ccacaaggta
    14821 cgactgttcg tgatgcggag actggcaagg ttttaacaga tttgattgaa catgggcaag
    14881 aatttatcgt tgcccacggt ggtcgtggtg gacgtggaaa tattcgtttc gcgacaccaa
    14941 aaaatcctgc accggaaatc tctgaaaatg gagaaccagg tcaggaacgt gagttacaat
    15001 tggaactaaa aatcttggca gatgtcggtt tagtaggatt cccatctgta gggaagtcaa
    15061 cacttttaag tgttattacc tcagctaagc ctaaaattgg tgcctaccac tttaccacta
    15121 ttgtaccaaa tttaggtatg gttcgcaccc aatcaggtga atcctttgca gtagccgact
    15181 tgccaggttt gattgaaggg gctagtcaag gtgttggttt gggaactcag ttcctccgtc
    15241 acatcgagcg tacacgtgtt atccttcaca tcattgatat gtcagctagc gaaggccgtg
    15301 atccatatga ggattaccta gctatcaata aagagctgga gtcttacaat cttcgcctca
    15361 tggagcgtcc acagattatt gtaactaata agatggacat gcctgagagt caggaaaatc
    15421 ttgaagaatt taagaaaaaa ttggctgaaa attatgatga atttgaagag ttaccagcta
    15481 tcttcccaat ttctggattg accaagcaag gtctggcaac acttttagat gctacagctg
    15541 aattgttaga caagacacca gaatttttgc tctacgacga gtccgatatg gaagaagaag
    15601 tttactatgg atttgacgaa gaagaaaaag cctttgaaat tagtcgtgat gacgatgcga
    15661 catgggtact ttctggtgaa aaactcatga aactctttaa tatgaccaac tttgatcgtg
    15721 atgaatctgt catgaaattt gcccgtcagc ttcgtggtat gggggttgat gaagcccttc
    15781 gtgcgcgtgg agctaaagat ggggatttgg tccgcattgg taaatttgag tttgaatttg
    15841 tagactagga gactggtatg ggagataaac cgatatcttt ccgagatgcg gatggtaatt
    15901 ttgtttccgc cgcagacgtt tggaatgaaa agaaattgga agaactattt aatcgtctca
    15961 atccaaatcg tgccttgaga ttggcacgaa ctaaaaagga aaatccatct cagtaaagaa
    16021 gctaaaaaat cccgtgccac atcaaacacg ggattttgtg gtacgacggg catgtatagc
    16081 gaactgaatc tggaatagca cagcatatct tctaaaatat agtaaaatga aatgagaaca
    16141 gaacaaatcg atcaggacag taaaatcgat t
//
LOCUS       DAVKJD010000039        16129 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKJD010000039 DAVKJD010000000
VERSION     DAVKJD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16129
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.039"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            113..3976
                     /gene="spuA"
                     /locus_tag="VW157_001270"
     CDS             113..3976
                     /gene="spuA"
                     /locus_tag="VW157_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9552568.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPATEPP
                     TPAASPADPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQ
                     GLWTWDDVEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNL
                     TGDKSVEKLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVK
                     NPSSAQWPDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFT
                     DLKNHSQIFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKH
                     SNITNHLGNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDE
                     TYSYDGKLGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTW
                     KQTLDSTNKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVA
                     KAAFVDPAKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEA
                     FIEKLDYLKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSL
                     TGMYSSDPKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFM
                     DADGTPRTSFGGGRLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEA
                     YKAARALNPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGY
                     PNEGQPAFITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSI
                     KKDPSKAENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDK
                     VPNKSHLLRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATGGKAYPENVKSRDYMKG
                     LIALRQSTDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVN
                     ADEKAREFNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLR
                     VSQNGTSHESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENK
                     PSQATADSQAEQPAQEAQASSVKEAVRNESVENSSKENIPATPDRQAELPNTGIKNEN
                     KLLFAGISLLALLGLGFLLKNKKEN"
     gene            complement(4076..5125)
                     /locus_tag="VW157_001271"
     CDS             complement(4076..5125)
                     /locus_tag="VW157_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9552569.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            5182..5259
                     /locus_tag="VW157_001272"
                     /pseudo
     CDS             5182..5259
                     /locus_tag="VW157_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(5396..7204)
                     /gene="glmS"
                     /locus_tag="VW157_001273"
     CDS             complement(5396..7204)
                     /gene="glmS"
                     /locus_tag="VW157_001273"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9552570.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(7396..8775)
                     /gene="bglA"
                     /locus_tag="VW157_001274"
     CDS             complement(7396..8775)
                     /gene="bglA"
                     /locus_tag="VW157_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9552571.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            complement(8875..10728)
                     /locus_tag="VW157_001275"
     CDS             complement(8875..10728)
                     /locus_tag="VW157_001275"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9552572.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPTLLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(10741..12000)
                     /gene="rseP"
                     /locus_tag="VW157_001276"
     CDS             complement(10741..12000)
                     /gene="rseP"
                     /locus_tag="VW157_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9552573.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(12022..12825)
                     /locus_tag="VW157_001277"
     CDS             complement(12022..12825)
                     /locus_tag="VW157_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9552574.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKIRGLATMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(12834..13592)
                     /locus_tag="VW157_001278"
     CDS             complement(12834..13592)
                     /locus_tag="VW157_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9552575.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALIKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(13823..14395)
                     /locus_tag="VW157_001279"
     CDS             complement(13823..14395)
                     /locus_tag="VW157_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU9552576.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDFETDVDVIFFDPDISYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTAFYTSSRDAMSKYPERCTAVGLRLNEESDFELYVPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLFKKNWQEKWKNLIFKNT"
     gene            complement(14376..15347)
                     /gene="ruvB"
                     /locus_tag="VW157_001280"
     CDS             complement(14376..15347)
                     /gene="ruvB"
                     /locus_tag="VW157_001280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9552577.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            15508..15600
                     /locus_tag="VW157_001281"
     CDS             15508..15600
                     /locus_tag="VW157_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9552578.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         4900 a         3802 c         3068 g         4359 t
ORIGIN      
        1 tacagaaaac ttctcaaaag taagaatttt tttcacaaat caaagaaaac gcttgcatat
       61 tgttttacta catgctatac tgatatagaa atttattgat tggagaatca ttatgagaaa
      121 aacaccatct cacactgaga aaaaaatggt ctacagcatc cgttccctca aaaatgggac
      181 tggttctgtc cttatcggcg caagccttgt cttgcttgcc atggctacac caactatctc
      241 atccgacgaa agtacaccaa ccactaacga acccaacaac agaaatacaa ccacccttgc
      301 ccaacctctt actgatacag cagctggctc tggtaagaac gaaagtgata tttcttcacc
      361 tggaaatgca aacgcttccc tagagaaaac agaagaaaaa cctgcaacag agccaccaac
      421 tcctgctgca agcccagccg atccagcacc acaaactgga caagatcgtt caagtgagcc
      481 aactacttct actagtccag taacaactga aactaaggca gaagagccca tcgaagataa
      541 ctacttccgt atccatgtca aaaaacttcc tgaagaaaac aaggatgctc aaggactatg
      601 gacttgggac gatgttgaaa aaccatctga aaactggcca aacggagctt tgtccttcaa
      661 ggatgccaag aaagatgact acggctatta cctagatgtc aaattaaagg gagaacaagc
      721 caagaaaatt agcttcctca tcaacaatac agctggaaaa aatctaaccg gcgataaatc
      781 tgtagaaaaa ctagttccaa aaatgaacga agcttggtta gaccaagatt acaaggtttt
      841 ctcttacgag ccacagcctg caggaactgt tcgcgtcaac tactaccgca cagatggcaa
      901 ctatgacaag aaatctctct ggtactgggg agatgtgaaa aatccaagta gcgctcaatg
      961 gcctgacgga acagacttta cggctacagg caaatatggc cgctatatcg acattcctct
     1021 taatgaagcc gcaagagaat ttggattttt attactagat gagagcaaac aaggagacga
     1081 cgtgaaaatc cgtaaagaaa attataagtt cacagatttg aaaaatcata gccaaatttt
     1141 cctaaaagac gatgatgaat cgatttacac aaatccatac tatgtccatg atatccgtat
     1201 gacaggagcc caacacgtag gcacttctag cattgaaagt agcttttcaa cacttgtcgg
     1261 tgctaaaaaa gaagatatcc tcaaacactc caacatcact aatcacctag gaaacaaggt
     1321 aactattacc gatgttgcaa tcgatgaagc tggtaagaaa gtgacctaca gcggagattt
     1381 ctctgacaca aaacatcctt atactgttag ctacaattcc gaccaattca ctaccaaaac
     1441 aagctggcgc ctgaaagatg agacatacag ctatgatggc aaactgggag ctgacctaaa
     1501 agaagaagga aaacaagttg atttgaccct ttggtcacca agtgctgata aggtttctgt
     1561 tgttgtctac gacaagaatg accctgacaa agtagttgga actgtcgctc tcgaaaaagg
     1621 ggaaagagga acttggaaac aaactctaga cagcacaaac aaactcggaa ttacagattt
     1681 cactggctac tattatcaat accaaatcga gcgtcaaggt aaaactgttc ttgcactcga
     1741 tccttacgct aaatctcttg ctgcttggaa tagcgacgat gccaagattg acgatgccca
     1801 taaagtggct aaagccgcct ttgtagatcc agctaaactc ggacctcaag acttgactta
     1861 tggtaagatt cacaatttca agactcgtga agacgccgtt atctacgaag ctcatgtgcg
     1921 tgacttcact tcagatcctg ccattgcaaa agacttgacc aaaccatttg ggacttttga
     1981 agccttcatt gaaaaactag actatctcaa agacttgggt gtaacccata tccagctcct
     2041 tccagtcttg tcttactact ttgtcaatga attgaaaaac catgaacgct tgtctgacta
     2101 tgcttcaagc aacagcaact acaactgggg atatgaccct caaaactact tctccttgac
     2161 tggtatgtac tcaagcgatc ctaagaatcc agaaaaacga atcgcagaat ttaaaaacct
     2221 catcaacgaa atccacaaac gtggtatggg agctatccta gatgtcgttt ataaccacac
     2281 agccaaagtc gatatctttg aagacctaga gccaaactac taccacttta tggatgccga
     2341 tggcacacct cgaactagct ttggtggtgg acgcttgggg acaacccacc atatgaccaa
     2401 acggctccta gttgactcta tcaaatacct agttgatacc tacaaagtgg atggcttccg
     2461 cttcgatatg atgggagacc atgacgccgc ttctatcgaa gaagcttaca aggctgcacg
     2521 cgccctcaat ccaaatctaa tcatgcttgg tgaaggttgg agaacctatg ccggtgatga
     2581 aaacatgcct actaaagctg ctgaccaaga ttggatgaaa cataccgata ctgtcgctgt
     2641 cttttcagat gacatccgta acaacctcaa atctggttat ccaaacgaag gtcaacctgc
     2701 ctttatcaca ggtggcaagc gtgatgtcaa caccatcttt aaaaatctca ttgctcaacc
     2761 aactaacttt gaagctgaca gccctggaga tgtcatccaa tacatcgcag cccatgataa
     2821 cttgaccctc tttgacatca ttgcccagtc tatcaaaaaa gacccaagca aggctgagaa
     2881 ctatgctgaa atccaccgtc gtttacgact tggaaatctc atggtcttga cagctcaagg
     2941 aactccattt atccactccg gtcaggaata tggacgtact aaacaattcc gtgacccagc
     3001 ctacaagact ccagtagcag aggataaggt tccaaacaaa tctcacttgt tgcgtgataa
     3061 ggacggcaac ccatttgact atccttactt catccatgat tcttacgatt ctagcgatgc
     3121 agtcaacaag tttgactgga ctaaggctac aggtggtaaa gcttatcctg aaaatgtcaa
     3181 gagccgtgac tatatgaaag gtttgattgc ccttcgtcaa tctacagatg ccttccgact
     3241 taagagtctc caagatatca aagaccgtgt ccacctcatc actgtcccag gccaaaatgg
     3301 tgtggaaaaa gaggatgtag tgattggcta ccaaatcact gctccaaacg gcgatatcta
     3361 cgcagtcttt gtcaatgcgg atgaaaaagc tcgcgaattt aatttgggaa ctgcctttgc
     3421 acatctaaga aatgcggaag ttttggcaga tgaaaaccaa gcaggatcag tcggaattgc
     3481 caacccgaaa ggacttgaat ggactgaaaa aggcttgaaa ttgaatgccc ttacagctac
     3541 tgttcttcga gtctctcaaa atggaactag ccatgagtca actgcagaag agaaaccaga
     3601 ctcaacccct tccaagcctg aacatcaaaa tgaagcttct caccctgcac atcaagaccc
     3661 agctccagaa gctagacctg attctactaa accagatgcc aaagtagctg atgcggaaaa
     3721 taaacctagc caagctacag ctgattcaca agctgaacaa ccagcacaag aagcacaagc
     3781 atcatctgta aaagaagcgg ttcgaaacga atcggtagaa aactctagca aggaaaatat
     3841 acctgcaacc ccagatagac aagctgaact tccaaataca ggaatcaaaa acgaaaacaa
     3901 actcctattt gcaggaatca gcctccttgc gctccttggt ctcggtttct tactaaaaaa
     3961 taaaaaagag aactaaacta gccctcctat agaaaaatcc cccaagcatt atagctcggg
     4021 ggattaattt tcgtacaata tttgttgtcc taataaactt gattaggatt ttttattaag
     4081 cctctttcat agcaaaataa gctcgtactt tgggtgcaac ttgtgttccg aagagttcaa
     4141 tagctctcag aacctggtca tgaggcatag aaccaagcgg tagatgaagc atgaagcggt
     4201 ccaatcctaa atcctctatc atgcgaatca atttttcggc cacctgatct ggattgccaa
     4261 caaacatggc gccatttgac cctacctgct ccaaatattg ctcataacgc aattcctgcc
     4321 agtgcggacg gtctttggaa atagcatcca ccacttgctt agtcggatgg aaataatctt
     4381 tcaccgcctg ctcaccatct tccgcaatcc acccccaaga atgggctccc actttcaagt
     4441 ctttgtcagc atggcccgct tcgcttccaa tctcacgata agcctgaatc aactttttaa
     4501 aataacgtgg attaccacca ataatagcat atacaatcgg tagaccagcc tgagcaatct
     4561 tcactgttga ttcgacatga ccacctgtag ctatccacaa gggcaatttg tcctgaactg
     4621 gacgaggata aacttcttta ccagcaatcg tttgagtcaa tcgaccttgc cagtctaact
     4681 tggtcttttc attgactaac tgaagcaagt ctaatttctc atcaaaaaga gagtcgtagt
     4741 ctttcaagtc ataaccaaac agagggaaag attccgtgaa agagcccctt ccagccataa
     4801 tctccgatcg tccatttgac aaagcatcga tagtggcata ctgttggaac aaacgaatcg
     4861 ggtccatgct tgacagaatg ctgactgcac tggtcaaacg gattttcttg gtattgactg
     4921 ccccagcggc caggacaatc tctggggctg atactgcaaa atccgcccga tggtgctcgc
     4981 caatcccata tacgtccaaa ccaaccttgt cagccagctc aatctctgcc accaactggc
     5041 gaatgcgttc agcatgactg taagtttgtc cagtcccttc aagctccgtt atttccccaa
     5101 atgttgaaat tcccaattct accattgtga ttctccttgt ctatctctgt ccttcaattt
     5161 gaaaaattat tctaatactc aatgaaaatc aaagagcaaa ctaggaagct agctgcaggc
     5221 tgctcaaaac agtgttttga ggttgtggat agaactgacg aagtcagtaa ccacacctac
     5281 ggcaaggcga cgttgacgtg gtttgaagag attttcgaag agtataacac gaatcttaag
     5341 tacaagcaac caatttgctc attagaaaaa gcctagataa ctagactttt ttagcttatt
     5401 ctaccgttac tgatttagca aggttacgtg gtttatccac atcgaggcca cggtggaggg
     5461 ttgcaaagta agcgactaat tgcgttggta cgaccattga aattggtgag aggtatggat
     5521 gtacggtcgt aaggacgata tcgtcggtat ctttggctac attctcttct gcgatagtga
     5581 ggactttggc accacgggct gcgacctctt ggatatttcc acgagtatga ttggcaagaa
     5641 ctggatctga caagagagcc aaaacaggcg ttccttcttc aatcaaggca atggttccgt
     5701 gcttgagttc tcctgcagca aagccttcac actggatata agaaatctct ttgagtttga
     5761 gacttgcttc catggctacg tagtaatctt gaccacgtcc gatgtaaaag gcgttacgag
     5821 ttgtttcaag aagttcacga accttgactt caatggtttc tttctctgaa agagttgatt
     5881 cgatagactg agctacgatt gacaattcat gaaccaggtc aaaggcttgc gctttagcat
     5941 taccatttgc ttctccgact gcttttgcaa ggaaggcaag ggctgcgatt tgcgctgtat
     6001 aggctttagt tgatgccacg gcaatttcag gacctgcgtg aaggagcatg gtatagttgg
     6061 cttcacgtga gagggttgaa cctggaacgt ttgtcactgt taagcttgga attcccattt
     6121 cattagcctt gaccaaaact tgacgactat ccgctgtttc accagattgg ctgataaaga
     6181 tgaagagtgg tttcttgctg agaagtggca taccgtagcc ccactcagat gagattccaa
     6241 gttcaactgg tgtatctgtc aattcttcca acattttctt agaagcaaat cctgcatggt
     6301 aagatgttcc agctgcaagg atgtagatgc ggtctgcgtc ttgaacagcc ttaatgatat
     6361 ctgggtctac gacaacttga ccagcctcat ctgtgtaggc ttggatgagt ttccgcataa
     6421 cagttggttg ctcgtcaatt tccttgagca tgtagtaagg gtaagttccc ttaccgatat
     6481 ctgacaagtc aagttcagca gtgtagctag cacgctcacg acgatttcca tcatagtctt
     6541 gaacttccac actatcagcc ttgacgatta ccaactcttg gtcatggatt tccatgtatt
     6601 ggttagtttc acgaatcata gccatggcgt ctgagcagac catgttatag ccttctccaa
     6661 gaccaatcaa aagtggtgat ttatttttag ctacgtagat gacttcagga tcttgtgagt
     6721 caaccaaggc aaaggcataa gaaccacgga tgatgtgaag ggcttttttg aaggcttcaa
     6781 gaactgagag cccttcttct tccgcaaatt ttccaatcaa atgaacggct atttcagtat
     6841 ctgtctgccc cttgaagtgg tgacctgcaa ggtattcttc cttgatttca agatagttct
     6901 caatcacccc attatgcacc aagacaaaac gttccgtctc agagcggtgt gggtgagcat
     6961 tgtcctcagt tggttttccg tgagtagccc aacgagtatg tccgatacca gttgttccct
     7021 caacaccagc tgtcttggca gacaattctg caatacgacc aaccgccttc accaaatggt
     7081 tatcagcacc atctaggaca aaaattcccg cagaatcata gccacggtat tcaagctttt
     7141 caagcccttg aatcaaaata tcagttgcat ttgtgtttcc aacaacacca acaattccac
     7201 acatagtata tacgacacag gcaagctgtg ctttctcctt aaaattggta tagtctaatt
     7261 catcttttat agaatcagca aaaacagtat atacttgttt ctttcacttg tcaagagtaa
     7321 aaattggtat agttcaaatt aagctcctgt aagcaaaaaa ctctgaccga ttgggataat
     7381 cagtcagagt cctttttaaa atccattatt atcgcttaat tttttgaacc agtggcctga
     7441 tttcttcaga cgacgttctt gcgtttccaa gtctaattcg accaaaccat agcgattttt
     7501 atagctgttg agccatgacc agcagtcaat aaaggtccaa atcaagtagc ccttacagtt
     7561 ggcaccatct tcaatggcac ggtgaagttc acgaagatga ccttttacaa agtcaatacg
     7621 gtaatcatct tgaatcattc catcttgacg gaatttttct tccccttcaa cacccatacc
     7681 attctcagtc aacatccact caatattgcc ataattttcc ttgatatttt gggcgatgtc
     7741 ataaatccct tgctcataaa tctcccaacc acgatgagaa ttgattttac gtccaggcat
     7801 cacataaggc tcgtaaaaat gttctggtaa gagtggactc tctggatgct tagcaaatcg
     7861 aggagccata acacgcaaag gttgatagta gttcacacca aggaagtcca ccgtattatc
     7921 acgaatgagt tccaactctt cctctgtagc atcaggtaaa agaccgtgtt catgcaagat
     7981 ttctaccaac tcctgtggat aagtccccaa gacagatgga tctaagaaag attgggcctg
     8041 aaaaagggcc gcaatacgag ctgccttgac atcagcagga tgctggctac gtggataagc
     8101 cggtgtcaag ttgaggacaa tcccaatctt ggaatcaggc aaaagttcat ggcaagcctt
     8161 aacagcccgg ctgctggcca attgtgtatg ataggctacc ttaacagctg cctctgcatc
     8221 caccttatgt ggataatggg catcataaaa ataaccaaat tctacaggaa cgatgggctc
     8281 gttaaaggta atccattgat ccactaaatc tccataagtc tcaaaacaaa aacgagcata
     8341 gtcttcatag gctgagactg tcgccttatt ttcccaacca tcaccatcct cttgaagggc
     8401 aaaaggtaaa tcaaaatgat agagattgac taacagacga attcctttag ccttaatagc
     8461 ctcaaagacc ttacgataaa aatccacacc ttgagggttg acttttccac agccttgtgg
     8521 aaaaatccgt gaccactgaa tagaagtccg aaagactgtg tgaccagtct ctaacaaaag
     8581 ctcaatatcc cgctcccaat tttcataaaa agtcgatgtc ttatctgaac caatcccatt
     8641 atagtaacga tttggctcca cttggaacca gtaatcccag agattgtctc ccttaccgtc
     8701 accagctaca cgtccttctg tctgcggtcc agaagtagag gatccccaga caaaatcctt
     8761 tggaaatctt agcataacat ttacctcttt atctactcat ttctcccatt atacagaaaa
     8821 aacaaggtaa aaactagtta cattttttcc ttgtttttct tctgattata gtttttattt
     8881 cttgcttagg atttcaagcg tttcaagcac gttatctgca tgaacctcaa tggtgtcacc
     8941 agttgccttg atcttaactt ctacaatgcc atcggccgct tttttaccaa cagtgatacg
     9001 gattggaaga ccaatcaagt cactatcgct aaatttaaca ccgacacgtt cgttacggtc
     9061 atctgtcaag acttcataac cagctcccat caagcttgct tcaagttttt ctgtcaaggc
     9121 ttgcgcttct tcatccttga cattgacagt aatcaaatgc acatcaaatg gtgccaattc
     9181 tttagggaaa ttgattcccc aagcgtaacg gtattcacct tttggcgttt tgttaacaaa
     9241 gaggcgagcg tgttgctcca tcactgctga aagaagacgg ctgacaccga taccgtaaca
     9301 tcccatgatg attggcacag cacgaccatt ttcatccaag acatctgctc ccatgcttgc
     9361 tgagtagcga gttccgagtt tgaaaatatg accgatctca ataccacgcg caaagttaag
     9421 gacaccttgt ccatctgggg aaatttcacc ctcacgaact tcacggatat ccacatattc
     9481 tgcagtaaaa tcacggcctg ggttcacacc agtcaagtgg tagccatctt cgttagcacc
     9541 gacaactgca ttgcgaacat cttgcacctt acgatctgca ataattttaa tattctctgg
     9601 caaaccaact ggtccaagtg aaccaaatcc tgcttgaaca acattcgcca cttcttcttc
     9661 gctagcaacg tcaaagaaat ctgctcccaa gtgatttttc aacttgactt cgttgagttg
     9721 gtcatttcca actagaaggg ctgcaacaag ctcaccatct gcaatgtaga agagggtttt
     9781 aatcgtttgt tcttctggaa cattgaggaa ggctgcaact tcatcaattg atttaacatc
     9841 tggcgttgca acacgagtaa cttcttcttc agcgacaaca cggttgcttg gtttgtactc
     9901 gtttgttgcc atttctaagt tagctgcata gctagactca cttgagtaag caatggtatc
     9961 ttcaccagag actatccatt tgagcaattc tgccttgatt tcttcttgca cttctgcagg
    10021 aatttcgtca aatgaggcaa ctgacttgtc caagacaacc cagcggtcaa ggtctgtacg
    10081 agcagatgta atggccataa attcttggct atccttacca cccatggctc caccgtcacc
    10141 aataatagcc ttgaagtcta aaccactacg agtgaaaata cgctcatagg ctgctttgta
    10201 ctcatcataa acactatcca aactatcata gttagcgtgg aaactataag catccttcat
    10261 gataaactca cgtgtacgaa gaagtccatt acgtgggcgt ttttcatcac gatacttggg
    10321 ctgaatttga taaaggttga gtggcaattg cttgtaagat ttaacagaat cacggacaat
    10381 agctgtaaag gtttcttcgt gagttggacc taagataaag tctgattttt cacggttttt
    10441 tagtttgtaa aggtcttcac cataggtttc gtaacgacct gattcacgcc acaattctgc
    10501 actaagaagg gttggagcca acatctcaac agcaccaatc ttttcgaatt cttggcgcat
    10561 gatgttttta gctttttcaa tcacacggtt ggcaagtggt agataagaat aaacacctgc
    10621 tgaaacttgg cgaacataac cagcacgcaa cataagagca tggctgataa cttgagcatc
    10681 gcttggcatt tcgcgaagcg ttgggatagg cattttactt tgtttcataa tattcctcga
    10741 ttatctaaaa aagagtcgca taatgtcatt ccaagtcaca gcaatcatca agacaaccat
    10801 gatgaccact ccggccaagg tgacataggt ttcaatttct tgtttcaatg gtttgcggcg
    10861 gatggcttct aggatattga gcacaatctt accaccatcc aaggctggaa tcggaataag
    10921 attaaaaatc ccaatattga tggaaatcat tgccaagaag tacaagatat tctcaattcc
    10981 atttttagca gcatcactac ttgccttaaa gatagcaaca ggtccaccca acttgttcaa
    11041 atccggttgg aaaatcagat ttttcagagc tgagagaatt cggagagctg agtcagcagc
    11101 agttgtaaaa ccacctacaa acatggatag aaaatctgac ttaacccccg gttgaacacc
    11161 tagaaggtaa cgaccttgac tatcttcggg tgtaacagtg acttgtttgt cactcccctt
    11221 ttcagaaata gtcacatcca aagtcggtgc cgtcttatct ttggtttctg tttccacagc
    11281 ttggatcaag ctttcccagt tgctaacctc atgtgagcca atcttggtaa tttgtgccgt
    11341 ttctggtact cctaccttgg ccaaggcacc ttggggcatg atatggaact gattggtatc
    11401 aacatctctg acaccaccct gcataaagat taaaacccaa aaaacaacga cacctaagat
    11461 aaaattgttc ataggacctg caaaattggt aatcagtttg ccccagatag tcgcattttg
    11521 atattgaaca tctaaaggtg caatccgaac ctcagtacca tctgcttcca caaccgttgc
    11581 atcgtgatcc actgcaaatg ttttttcttc ttccagaacc aatcctttga taaagagctt
    11641 gtcttcaaaa tcaaactggg tcacctgcat agggagggct gtttgatcca attttttacc
    11701 tgagagattg atgcgtttaa ccttaccatc atcagcaagt gtcaaactaa caggcgttcc
    11761 tgtcttgatt tcagttgtat catcacccca accggccatg cggacatagc cacccagagg
    11821 caagattcga atggtatagg ccgttccatc cttgccaatg tgagcaaaaa ttttaggtcc
    11881 cataccgatg gcaaattcac gtactaaaat ccctgatttc ttggcaaagt agaagtgccc
    11941 gaactcgtgc accactacaa taatcccgaa aaccagaata aaggttaaaa ttccgagcat
    12001 agcgtttcct ccgtcttttg attaaaagag tccaaataag tgcatgattg gaaatacaag
    12061 caacatacta tcgaaacgat ccaaaacacc accatgtcca gggataaatt tcccagaatc
    12121 cttaacacca aaatgacgtt tgatcgaact ttctagtaaa tcaccaaatt gtccagcaat
    12181 gctaaagaaa atagcaaaga ctgacatctt gtaaattcca tatggaagag caactgtact
    12241 gtcaactatc ataaagataa tggttactaa aattgctcct aaaataccac ccaaggcacc
    12301 ctcaagggtt ttattaggcg atacccttgg tgctaacttt cgtttcccat agttcatccc
    12361 aacaagatag gcaccactgt ctgtcgccca gacgatacac aaggctaaga gagccttgtc
    12421 caaacctgca acacgagcat ctagtaaagc attaaatcca aagcccacgt agaagctcat
    12481 agcaagaggg aaaaccgcat cctcaatcgt ataagacttg ctaaaaacgg tcgttcctaa
    12541 catgattgaa atcaaaacac tataggcaac cacattccca tcaactggca aaaaagtcag
    12601 gtaattctcc aagggaatgg ttaatgcaaa ggttgcaaag agagtcaaga ggccctccat
    12661 cgtcatggtc gctagacctc ttatcttcaa aagttcatgc atggctagca tggctatgat
    12721 tccgattgct atctgaagca agaggccccc aatcattaaa attggtagga aaatagccag
    12781 ggcaatccct gcaaacaagg ttcttttctg taaatcctgg gtcatatttc ctcctaaact
    12841 cctccaaatc ggcgatggcg acgattatag gcaagaatag cttcctgcaa ggctgcttcg
    12901 tcaaaatcag gccataaggt gtccgtaaaa taaagctcac tataggctcc ctgccatgga
    12961 aggaaattgc tcaaacgcaa ttctccacta gtacggataa tcaagtctgg gtctcgtaag
    13021 tccttaggca aatgctgggt aaagagatag ttgccaatca attcctctgt gatgtcacct
    13081 gggttgattt tggcatctaa aacatcctgg gaaatcaact taagcgcctg tgtaatctca
    13141 gcacgtccac catagttaag agcaaaatta agaatcaatc ctgtgttgtt cttagtcaat
    13201 tcctcagcct tgattaaagc ttcaaaggtt tgcttaggca ggcggtctgt ctccccaatc
    13261 atttgaatct taacattatt cgcatgtagt tccgggacat aattatcata aaactctact
    13321 ggcaagttca tgataaactt gacttcctga tctggacggg tccagttttc cgtagaaaaa
    13381 gcatagaccg taataacctt gacgcccagt ttgttggctg ccttggtcac ggtttgcaat
    13441 gcttccatgc ccgccttatg tccaaaaact cgcggttgca tacgtttttt agcccaacgg
    13501 ccattgccat ccatgatgat gccgatatga gcaggaacct gtgtcggaac ctctacttcc
    13561 acagccttat ctttcttaaa aaatccaaac atgatcttat tcctattcaa aaatctatcg
    13621 tttcattata ccatatttcc ccattttctt ctatcactaa gctatttatt ctcaggcacc
    13681 aagcccattt ttcaaaaaaa taagccgcct gattgggcga ctttattttt atagggagat
    13741 tattatgaaa aagttttagg agtttaagtt aaggttttct taacttatga acttagtgta
    13801 cactccctag cttaaagttt ccttaagtat ttttaaaaat caaatttttc catttctcct
    13861 gccaattttt cttgaataaa cgtgtttgat agagttccat tcggtcttca ttttctaaga
    13921 aatgaggagt tggacgaact tgaaaattca aaatatcctc caaaccataa ggtacataga
    13981 gttcaaaatc tgattcttca ttcaagcgca gtccaactgc cgtacaccgt tctggatact
    14041 tactcatagc atcacgagaa ctggtataga aagcagtgtg aggactgtgc tgatgcatat
    14101 agacctgatt tttcaattcc cactggtact gaggaaaatc ctctctcagc tttttctcca
    14161 gtaataaggt ttcctcataa gaaatatccg gatcaaagaa aatcacatct acatctgttt
    14221 caaagtcaaa aggtgatttg tctgacaaga gattccagat gaaatttctg acagaacctg
    14281 ctgccaacca cgagtctttc agaccaaggt ctcggatgat cgtcagaatg gccatcatat
    14341 ctggattttc tctaaaagcc tctaagattt cttgcttatt tttcactgta ttcataacct
    14401 aagtgctcat atgccttagc agtcgccacc cgtccagacc gtgtccgcat gataaaacct
    14461 ttttgaatca agtaaggctc atacatatct tcaactgtct cacgctcttc ggcgatattc
    14521 acagaaagag ttcctagacc aacaggtcct ccactgtaca tctcaatcat ggtacgaagg
    14581 attttttgat ccacatagtc caaaccttca tggtcaacat ccagcatagt caaagcctta
    14641 tcggtaataa tatcatcaat taccccattc cccattatct gggcaaaatc gcgcacgcgc
    14701 ttgaggagac gattggcaat acgaggggtc ccacgactac gtagggccaa ctcagatgct
    14761 gcctcatgag tgatttccat ctcaaaaata tctgccgtcc gctcgacaat ttctgtcaag
    14821 tcagcatggg cataatactc catatggcct gtaatcccaa aacgtgcccg tagcggattg
    14881 gagagcatac cagcccgagt cgtcgcacca atcaaggtaa aaggtggtaa ctccaaatga
    14941 acactacgac taccctcacc agccccaatc ataatatcta tgtagaagtc ctccatagca
    15001 ctataaagca cctcttccac tgacattggc aaacgatgga tctcatcaat aaaaagtaca
    15061 tccccaggct ctaactcatt caaaatagct accagatctc cggctttttc aatgactgga
    15121 cccgacgtct gcttaagatt gactcccagt tcgttggcaa taacaaaggc catggtcgtt
    15181 ttcccgagac ctggaggccc aaataagagc acatgatcca gcgcttcatc ccgcattttg
    15241 gcagcttcaa taaagatttg tagctggtcc ttgaccttat cctgtccgat atattcacgt
    15301 aaatactgag gacggagcgt gcgttctact aactcctcat cccccattat ctcattatct
    15361 aaaattctac tcatggctct attatatcaa aaaaccaagc cacaaacaaa aaagccacct
    15421 gattgggtga ctcctaagtt tagcacttat gtggtataat attatacgac actgctacac
    15481 cgcctacgaa aggaggtgag atagcccatg atggaattag tactcaaaac tatcatcgga
    15541 ccaattgtgg tcggtgtcgt tcttcgtata gtcgataaat ggctaaacaa ggacaaatag
    15601 tgtcaaaaaa gacctcaagc ttatttggtc gtgagcttgg ggtcttttct agcctatgat
    15661 atagaactag tactcagttc ctttttatta tcccatagtt cacgaatttt gtcaaaggtt
    15721 tacattttct tcaaccgctg tacgacaaga cggttaagat taagagaacg ttagggattc
    15781 tatcaatttc atagaaattt tgatttcgta aacgaagaga caatcttaca tgtcacttct
    15841 catttaatac gccactacta gacaagcaaa atcattatta cagtagttcc agttcttcaa
    15901 ttaacagtca cttacaatca aattgagttt gaactagctg aagcgaccac agacctattt
    15961 cttagttata ttcgctaaaa aagtccccgc caattccccg accaaaatcc gaaaaatacc
    16021 gaaaaatatc gaaaaattat ttttagaata gtccccaaaa gcctgaaata gagctaaaaa
    16081 actccacctg attgggtgga gttaagggag attattatga aaaagaaaa
//
LOCUS       DAVKJD010000040        15324 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKJD010000040 DAVKJD010000000
VERSION     DAVKJD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.040"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..1252
                     /locus_tag="VW157_001282"
     CDS             <1..1252
                     /locus_tag="VW157_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9552579.1"
                     /translation="IFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMVSIL
                     GLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLFERL
                     LYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFIAAN
                     TIHLAIGTRNPFILSILFAFVYYFMREQAEKGKWIGFKEKLAIFVGSPILMLAMGVLN
                     YVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVLDYF
                     ARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIMELY
                     TDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSESFFN
                     LFTMQFWGIVLVIIFVAKMLTKEN"
     gene            1281..2315
                     /locus_tag="VW157_001283"
     CDS             1281..2315
                     /locus_tag="VW157_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9552580.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            2515..3252
                     /gene="mecA"
                     /locus_tag="VW157_001284"
     CDS             2515..3252
                     /gene="mecA"
                     /locus_tag="VW157_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9552581.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            3403..4689
                     /locus_tag="VW157_001285"
     CDS             3403..4689
                     /locus_tag="VW157_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9552582.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            4691..5560
                     /locus_tag="VW157_001286"
     CDS             4691..5560
                     /locus_tag="VW157_001286"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9552583.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            5636..5707
                     /locus_tag="VW157_001287"
                     /pseudo
     CDS             5636..5707
                     /locus_tag="VW157_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            5855..6793
                     /gene="msrB"
                     /locus_tag="VW157_001288"
     CDS             5855..6793
                     /gene="msrB"
                     /locus_tag="VW157_001288"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9552584.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            6851..8575
                     /locus_tag="VW157_001289"
     CDS             6851..8575
                     /locus_tag="VW157_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552585.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLLPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYHHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VLVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQVTSDRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLILGLYPVNKGRIDL
                     YLDGLSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIREDLPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            8577..10325
                     /locus_tag="VW157_001290"
     CDS             8577..10325
                     /locus_tag="VW157_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552586.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFFLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            10672..11931
                     /locus_tag="VW157_001291"
     CDS             10672..11931
                     /locus_tag="VW157_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9552587.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPSGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    12007..12142
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            12181..12681
                     /locus_tag="VW157_001292"
     CDS             12181..12681
                     /locus_tag="VW157_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9552588.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            12757..13125
                     /gene="rplL"
                     /locus_tag="VW157_001293"
     CDS             12757..13125
                     /gene="rplL"
                     /locus_tag="VW157_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9552589.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            13312..13482
                     /locus_tag="VW157_001294"
     CDS             13312..13482
                     /locus_tag="VW157_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552590.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            13482..13733
                     /locus_tag="VW157_001295"
                     /pseudo
     CDS             13482..13733
                     /locus_tag="VW157_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            13720..14067
                     /locus_tag="VW157_001296"
                     /pseudo
     CDS             13720..14067
                     /locus_tag="VW157_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14060..14440
                     /locus_tag="VW157_001297"
     CDS             14060..14440
                     /locus_tag="VW157_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9552591.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            14453..14596
                     /locus_tag="VW157_001298"
     CDS             14453..14596
                     /locus_tag="VW157_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552592.1"
                     /translation="MAERFWENLSIILAERNISCIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            14689..15276
                     /locus_tag="VW157_001299"
     CDS             14689..15276
                     /locus_tag="VW157_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9552593.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
BASE COUNT         4580 a         2810 c         3312 g         4622 t
ORIGIN      
        1 tattttctat ttaaccattt ttgtatttct ggtatcaaga ccgaccattg attattttag
       61 ggatggtgct ttggatacct atcatccaat agcctatcgt tttgccttta tagttgtcat
      121 ggtttcgatt ctgggcttga caacaggagg cgtcctggct cgttacttca tagctaggaa
      181 gaaaataaaa gtagcaaata taggaaattc tctaaaagag gtttatatca agcggttacg
      241 ctttgtatca ctaggagttt ttcttctaac ttatcctttc tatttcattc ggttatttga
      301 acggctcttg tatcgtttgc agacttccta ctatgcctac tatgcaaatt ttgaaagtaa
      361 actgccttat tttacctaca ttttgtctac ctttacggtc tatgcaatgt gtatgtatct
      421 ggcaaccaag ccaaagaaat tgcaggccac agcagtgctt gtctccttta ttgcagctaa
      481 cactattcat ttggcaattg ggacacgaaa tccctttatt ttaagtattt tatttgcttt
      541 tgtttattac tttatgcggg agcaagctga aaaaggaaaa tggattgggt ttaaagaaaa
      601 gttagcgatt tttgtaggtt ctcctattct catgttagcg atgggagtac tcaattatgt
      661 acgggataat gtccaagttt cccatacagg tttctgggat atcttacttg actttatcta
      721 taaacaaggg actagttttg gtgttctggc tcgaggtttt ctatttaaca gtagcctccc
      781 ttaccgagat ttccgtaatt ttacttttgg tcctgttctt gattattttg caagggggag
      841 tttgggagcc attttcggag gaaaagcctt tgaacataca accaatagtg tggaactagc
      901 tattgatagt aatagttatg cccacaatct atcctatctt gtcttgaaca aggaatactt
      961 gaaagggcat ggtatcggaa gtagttatat catggagttg tataccgact atggtatgat
     1021 tggagtcttt ctgcttagtt tcttactcgg cgtattattt atagccatgc tgcaagtagc
     1081 ctatcgctca aggacaatcc tatttgcttt atccctactc atcttgaata atctattctt
     1141 tatgccaaga agcagctttt cagaaagttt cttcaattta tttacaatgc aattctgggg
     1201 aattgttctt gtgattatat ttgtagcaaa aatgcttaca aaggaaaact agtatctact
     1261 aaacaaagga gaaaaaaatc atgtttgaac attattcagt agctgatttg tttgcaaatc
     1321 tttataaaaa gcgaaaagca aatattttag ctctcatcgc tttatttgct ctcattgctg
     1381 taccatttac aattaaagca gttaggaata aaaacactgt caaagataca acaagttatt
     1441 caacttatct tatctataaa atcactcctc caaaagagtc ggccaaaacg attttgaatc
     1501 atcaaattgg tggttatagt gatttttatg ggaaattgat tgatggtaat ttgaatggag
     1561 cttatctttt caatgatgta gaacccagtg agttgaaaaa aattgcaagt gaattagaca
     1621 cgacagaaac aaccttgaaa aattcaacga atgactattg gtggaaaaaa ttgaccgtct
     1681 actatatgat tgacgatgca ggggttggtg tgaaaatttt gacatcaagt aaagatgcca
     1741 atgacttgtt agagaaaaaa attgatgggt tgattgagaa atttaaacat gcctatgcaa
     1801 atgtgaaaat tgaaaaactg gaaaccatca actctaaaga attgaacgca aatggtgaaa
     1861 cagcgcttgg cttaaatgtg aaaaatctga ttcttcgttt agttgttatt ggagtggttt
     1921 gtgtgatttt ggttgtgatg ggaaatgtgt tagtttatct ctttaatcca acaatcaata
     1981 gagtaggtga tttttctcag tatcaaattg attttgtaac agagattaca acaattgcta
     2041 acctagcaga tgttttgtca tacaaaaata ctggacagga attgaccatc gttagctcaa
     2101 ataaagctat cctagataaa ttgaaacaga gtcaagaagc tttaaaagga atgcattttg
     2161 tagatttaca ggatgtatca tctcttttgg aaagagatac agtccttctt gttgaagagt
     2221 acggagtgac tcgttataag aaatttgagc aaagtcttca aattctcaga aacttaaatc
     2281 gttctatcct tggtgtagca acctttaaat tataagtatt ctgatgtata aggtcttcaa
     2341 aatcggctaa gagctgattt tgaaggcttt ttctatctgt ctgcagaaat tttcttttgg
     2401 agtctcctat agaaagaccc tagggaaaat ctatccgtat agaggagcct tcttttgata
     2461 aaatcgtaaa aatctagtat aatagataga actgaaagta tgaggttact agcaatgaaa
     2521 atgaaacaaa ttagtgatac aactttaaaa atcacgatgt ctttagagga tttgatggat
     2581 cgtggaatgg agattgctga ctttctcgtt cctcaagaaa aaacagaaga gttcttttat
     2641 gctatcttgg atgagctaga gatgcctgat agctttctgg atacaggcat gttgagcttc
     2701 cgtgtgactc caaaacctga taaggtagat gtctttgtga ccaagtcaaa gattgaccaa
     2761 aatctagatt ttgaagactt atcggatttg ccagatatgg aagaattggc tcaaatgtct
     2821 ccagatgaat ttatcaaaac cctggaaaaa agcatcgcag acaaaaccaa ggatgatatc
     2881 gaagccattc aatctcttga gcaagttgaa gccaaggaag aagagcaaga gcaggctgaa
     2941 caagaagctg agagtaagaa agaaccttac atctactaca tcctttcttt tgctaagttg
     3001 gctgacttgg tagtttttgc caagacagtg acttttgaaa tggaaacttc tgaactctac
     3061 aaaatgaacg agcgctatta tttgaccatt ttagtggata ttgaaaatca tccaagccca
     3121 tatccagctt ggctgttggc ccgtatgcgc gagtttgcag acgatagtga tatcagtcgc
     3181 tcagtcttac aagagtatgg tcaagtcttg atgagtcacg atgcagtgct caatctgcaa
     3241 aaaatcggct aatcctttct ggaaagctat tttcagattt taagaagagc gaatcgtctg
     3301 attcgttttt tctttttata ctgaaatagt gatttactat aataggaatt ttcacaaaat
     3361 tctgttataa tggctatatt agaaaatttc gaggagataa agatgacagt taaaattgct
     3421 ttactaggat ttggtaccgt tgcaagtggt gtgcctttcc tcctaaagga aaatggagga
     3481 aaaatcaatc aatcagcaca ttcagatatc aaagttgcta aggtattggt caaggatgaa
     3541 gatgaaaaaa atcgcttgct tgcagcaggg aatgacttta actttgtaac caatgtggat
     3601 gatattttat cagaccagga tattactatc gtagtggaat tgatggggcg tattgagcct
     3661 gctaaaacct ttatcactcg tgccttggaa gctggaaaac acgttgttac tgctaacaag
     3721 gaccttttag ctgtccatgg cgcagaattg ctagaaatcg ctcaagctaa caaggtagca
     3781 ctttactacg aagcagcagt tgctggtggg attccaattc ttcgtacttt agcaaattcc
     3841 ttggcttctg ataaaattac gcgcgtgctt ggagtagtca acggaacttc caacttcatg
     3901 gtgaccaaga tggtggaaga aggctggtct tacgatgatg ctcttgcgga agcacaacgt
     3961 ctaggatttg cagaaagcga tccgacgaat gacgtagatg ggattgatgc agcctacaag
     4021 atggttattt tgagccaatt tgcctttggc atgaagattg cctttgatga tgtagcccac
     4081 aagggaatcc gcaatatcac accagaagac gtagctgtag ctcaagagct tggttacgta
     4141 gtgaaattgg ttggttctat tgaggaaact tcttcaggta ttgctgcaga agtgactcca
     4201 accttcctac ctaaagcgca cccacttgct agtgtgaatg gcgtaatgaa cgctgtcttt
     4261 gtagaatcta tcggtattgg tgagtctatg tactacggac caggtgctgg tcaaaaacca
     4321 actgcaacaa gtgttgtagc tgatattgtc cgtatcgttc gtcgtttgaa tgatggtact
     4381 attggcaaag acttcaacga atatagccgt gacttggtct tggcaaatcc tgaagatgtc
     4441 aaagcaaact actatttctc aatcttggct ctagactcaa aaggtcaggt cttgaagttg
     4501 gctgaaatct tcaatgctca agatatttcc tttaagcaaa tccttcaaga tggcaaagag
     4561 ggtgacaagg cgcgtgtcgt tatcatcaca cacaagatta ataaagccca gcttgaaaat
     4621 gtctcagctg aattgaagaa ggtttcagaa ttcgacctct tgaatacctt caaggtgcta
     4681 ggagaataag atgaagatta ttgtacctgc aaccagtgcc aatatcgggc caggttttga
     4741 ctcggtcggt gtagctgtaa ccaagtatct tcaaattgag gtctgcgaag aacgagatga
     4801 gtggctgatt gaacaccaga ttggcaaatg gattccacat gatgagcgta atctcttgct
     4861 caaaatcgct ttgcaaattg taccagactt gcaaccaaga cgcttgaaaa tgaccagtga
     4921 tgtccctttg gcgcgcggtt tgggttcttc cagctcggtt atcgttgctg ggattgaact
     4981 agccaaccaa ctgggtcaac tcaacttatc agaccatgaa aaattgcagt tagcgaccaa
     5041 gattgaaggg catcctgaca atgtggctcc agccatttat ggtaatctcg ttattgcaag
     5101 ttctgttgaa gggcaagtct ctgctatcgt agcagacttt ccagagtgtg attttctagc
     5161 ttacattcca aactatgaat tacgtactcg cgacagccgt agtgtcttgc ctaaaaaatt
     5221 gtcttataag gaagctgttg ctgcaagttc tatcgccaat gtagcggttg ctgccttgtt
     5281 ggcaggagac atggtgaccg ctgggcaagc aatcgaggga gatctcttcc atgagcgcta
     5341 tcgtcaagac ttggtaagag aatttgcgat gattaagcaa gtgaccaaag aaaatggggc
     5401 ctatgcaacc tacctttctg gtgctgggcc gacagttatg gttctggctt ctcatgacaa
     5461 gatgccaaca attaaggcag aattggaaaa gcaacctttc aaaggaaaac tgcatgactt
     5521 gagagttgat acccaaggtg tccgtgtaga agcaaaataa agaatagaag ataggatggg
     5581 gaaactcttg accagagggt ttcatatcct ttttgtgaaa agaagtttat actcaatgaa
     5641 aatcaaagag caaactagga agctagccgc aggctgctca aaacagtgtt ttgaggttgt
     5701 ggatagaact gaagaagtca gctcaagaca ctgttttgag gttgcagata gaactgacga
     5761 agtcagtaac atatatacgg taaggtgacg ctgacgtggt ttgaagagat tttcgaagag
     5821 tattagttaa aaacgtgata aaggagaaat aaagatggca gaaatttatc tagcaggtgg
     5881 ttgtttttgg ggcctagagg aatatttttc acgcatttct ggagtgctag aaaccagtgt
     5941 tggctacgct aatggtcaag tcgaaacgac caattaccag ttgatcaagg aaacagacca
     6001 tgcagaaacg gtccaagtga tttacgatga gaaggaagtg tcactcagag agattttact
     6061 ttattatttc cgagttatcg atcctctatc tatcaatcaa caagggaatg accgtggtcg
     6121 ccaatatcga actgggattt attatcagga tgaagcagat ttgccagcta tctacacagt
     6181 ggtacaggag caggaacgca tgctgggtcg aaagattgca gtagaagtgg agcaattacg
     6241 ccactacatt ctggctgaag actaccacca agattatctc aggaagaatc cttcaggtta
     6301 ctgtcatatc gatgtgaccg atgctgataa gccattgatt gatgcagcaa actatgaaaa
     6361 gcctagtcaa gaggtgttga aggccagtct atctgaagag tcttatcgtg tcacacaaga
     6421 agctgctaca gaggctccat ttaccaatgc ctatgaccaa acctttgaag aggggattta
     6481 tgtagatatt acgacaggtg agccactctt ttttgccaag gataagtttg cttcaggttg
     6541 tggttggcca agttttagcc gtccgatttc caaagagttg attcattatt acaaggatct
     6601 gagccatgga atggagcgaa ttgaagttcg ttctcgttca ggcagtgctc acttgggtca
     6661 tgttttcaca gatggaccgc gggagttagg cggcctccgt tactgtatca attctgcttc
     6721 tttacgcttt gtggccaagg atgagatgga aaaagcagga tatggctatc tattgcctta
     6781 cttaaacaaa taaaacagag agtggggctt cccactttct tcatttctag aatatgaata
     6841 gaagggattt atgaaacacc tattatctta cttcaaaccc tacatcaagg aatcaatttt
     6901 agcccccttg ttcaagctgt tagaagctgt ttttgagctc ttgcttccca tggtgattgc
     6961 tgggattgtt gaccaatctt tacctcaggg agatcaaggt catctctgga tgcagattgg
     7021 cctgctcctt atctttgcag taattggcgt tttagtggcc ttgatagctc aattttactc
     7081 agcaaaggca gcagtaggct ttgctaagga attgacaaac gatctttatc atcatattct
     7141 ttccttgcca aaggacagca gagaccgtct gacaacttct agtttggtca ctcgcttgac
     7201 ttcggatacc taccagattc agactggtat caatcaattc ctgcgtctct ttttacgagc
     7261 gcccattatc gtttttggtg ccatttttat ggcttatcga atctcagctg agttgacttt
     7321 ctggttctta gtcttggttg ccattttgac cattgtcatt gtagggttat ctcgattggt
     7381 caatcctctc tacagtagtc tcagaaagaa aacggaccaa ctggttcagg aaacgcgcca
     7441 gcaattgcaa gggatgcggg ttattcgtgc ttttggtcaa gaaaaacgag agttacagat
     7501 ttttcaaacc cttaaccaag tttatgctag attacaagaa aagacaggtt tctggtctag
     7561 tttattaaca cctctgacct atctgattgt caatggaact cttctcgtta ttatctggca
     7621 aggctatatt tcaattcaag gaggagtgct cagtcaaggt gctctcattg ctcttatcaa
     7681 ttacctctta cagattttgg tggaattggt caagctagcc atgttgataa attccctcaa
     7741 ccagtcctat atctcagcca agcgaatcga ggaagtcttt gttgaggctc cagaggatat
     7801 ccattcagaa ttagaacaaa agcaagttac cagtgatcgg gttttacaag tccaagaatt
     7861 gacctttacc tatcctgatg cggcccagcc ttctctgaga gacatttcct ttgatatgac
     7921 tcaaggacaa atccttggta tcattggggg gactggttct ggtaaatcaa gcttggtgca
     7981 actcatactt ggactttatc cagtaaacaa gggaagaatt gacctttatc tagatggatt
     8041 gagtcctctt aatttggagc aatggagatc ttggattgcc tatgtgccac aaaaggtcga
     8101 actctttaag ggaactattc gttccaactt gactttaggt ttaaatcaag aagtgactga
     8161 tcaagaactc tggcaagcct tggaaattgc tcaagctaag gattttgtca gtgataagga
     8221 aggactcttg gatgctctag ttgaagcagg aggtcgaaat ttctcaggtg gacaaaaaca
     8281 aaggctgtct atcgcacgag cagtcttgcg ccaggctccg tttctcatcc tagatgatgc
     8341 gacctcggca ctggatacca ttaccgagtc caagctcttg aaagctatcc gcgaagatct
     8401 gccaaatatg agcttaatct tgatttctca acggacttcg acactccaga tggcggacca
     8461 gattctcctc ttggaaaaag gtgagttgct agctgttggc aagcacgatg acttgatgaa
     8521 atccagccaa gtctatcgtg aaatcaatgc atcccaacat ggaaaggagg actagaatga
     8581 aacgacaaac tgtaaaccag acgctcaaac gtttagccgt agatttagca agccatcctt
     8641 tcttcctttt cctagccttt ctaggaacta ttgcccaagt tggcttatca atttacctac
     8701 ctattctgat tgggcaggtc attgaccaag tcctagtggc tggttcatca ccagtttttt
     8761 ggcagatttt tctccagatg ctcttggtgg taataggaaa tactctggta caatgggcca
     8821 atcctctcct ctataatcgt ctaatcttct cttataccag agatttacgg gagcgaatca
     8881 tccataagct ccatcgttta ccgattgcct ttgtagatag acaaggtagt ggagagatgg
     8941 ttagtcgtgt aaccacggac atcgaacagt tggcagctgg cttgaccatg atttttaacc
     9001 aatttttcat tggtgttttg atgattttgg ttagtattct agccatgctc caaattcatc
     9061 tcctcatgac tctcttagtc ttgctgttga cgccactgtc catggtgatt tcacgcttta
     9121 ttgccaagag atcctatcat ctcttccaga agcaaacaga gacgagggga attcagactc
     9181 agttgattga agaatcgctt agccagcaga ctataatcca gtccttcaat gctcaaacag
     9241 aatttatcca aagattgcgt gaggctcatg acaactactc aggctattct cagtcagcca
     9301 tcttttattc ttcaacggtc aatccttcga ctcgctttgt aaatgcactc atttatgccc
     9361 ttttagctgg agtaggagct tatcgtatca tgatgggttc aaccttgacc gtcggtcgtt
     9421 tagtgacttt tttgaactat gttcagcaat acaccaagcc ctttaacgat atttcttcag
     9481 tgctagctga gttgcaaagt gctctggctt gcgtagagcg tatctatgga gtcttagata
     9541 gccctgaagt ggctgaaaca ggtaaggaag tcttgaccag tgaccaagtt aagggagcta
     9601 tttcctttaa acatgtctct tttggctacc atcctgaaaa aattttgatt aaggacttgt
     9661 ctatcgatat tccagctggt agtaaggtag ccatcgttgg tccgacaggt gctggaaaat
     9721 caactcttat caatctcctt atgcgttttt atcccattag ctcgggagat atcttgctgg
     9781 atgggcaatc catttatgat tatacacgag tatcattgag acagcagttt ggtatggtgc
     9841 ttcaagaaac ctggctcaca caagggacca ttcatgataa tattgccttt ggcaatcctg
     9901 aagccagtcg agagcaagta attgctgctg ccaaagcagc taatgcagac tttttcatcc
     9961 aacagttgcc acagggatac gataccaagt tggaaaatgc tggagaatct ctctctgtcg
    10021 gccaagctca gctcttgacc atagcccgag tctttctggc tattccaaag attcttatct
    10081 tagacgaggc aacttcttcc attgatacac ggacagaagt gctggtacag gatgcctttg
    10141 caaaactcat gaagggccgc acaagcttca tcattgctca ccgtttgtca accattcagg
    10201 atgcggattt aattcttgtc ttagtagatg gtgatattgt tgaatatggt aaccatcaag
    10261 aactcatgga tagaaagggt aagtattacc aaatgcaaaa agctgcggct tttagttctg
    10321 aataagccat tctcttttga aagtttctgg acgaaaaaag ttgccttcga gtgacttttt
    10381 tgttacaata gctagaaaaa ttgttcactg taatactcaa tgaaaatcaa agagcaaact
    10441 aggaagctag ccgtaggttg ctcaaagcac agctttgaga ttgtagataa gactgacgaa
    10501 gtcagttcaa aacactgttt tgaggttgca gatagaactg acgaagtcag ctcaaaacac
    10561 tgttttgagg ttgcagatag aactgacgaa gtcagtaacc acacctacgg caaagtgaat
    10621 ttgaagtggt ttgaagagag tacaacttgt cttttagaaa aggagcctat aatgaaagtc
    10681 tttcagcatg taaatatcgt gacttgtgat caagatttcc atgtttatct tgatggaatc
    10741 ttagcagtca aggattctca aatcgtctat gtcggtcaag ataagccagc gtttttagaa
    10801 caagctgagc agattataga ctatcaggga gcttggatta tgcctggttt ggtcaattgt
    10861 cacacccatt ctgcaatgac aggtctgaga gggatccgag atgacagcaa tctccatgaa
    10921 tggctcaatg actatatctg gccagcagaa tctgagttta ctcccgacat gactaccaat
    10981 gcggtcaaag aagccttgac agagatgctc cagtcaggaa caacaacctt taacgatatg
    11041 tataatccca gtggtgtgga tatccagcaa atttatcagg tggtgaaaac ttccaagatg
    11101 cgttgttatt tttctccgac tctcttttct tcagagacag aaacaactgc tgagactata
    11161 agcagaactc gatccatcat agatgaaatc ttaaaatata aaaatccaaa tttcaaggtt
    11221 atggtagcac ctcattctcc gtatagctgc agtagagact tgctggaagc gagtttggaa
    11281 atggcaaaag agctaaatat tccgctccat gtccatgtgg cggagaccaa ggaggagtca
    11341 ggaattattc tcaaacggta cggcaaacgc ccccttgctt ttcttgaaga actgggttat
    11401 ttagatcatc cgtccgtatt tgctcacggg gtcgaattaa acgagagaga aattgaacgc
    11461 ttggcatctt ctcaagtggc tatcgcccac aatcctatca gtaacctcaa actggcatca
    11521 ggaattgcac caattatcca gctccaaaaa gcgggagtag cagtcggaat tgcgactgac
    11581 tcggttgctt ccaataacaa tctagatatg tttgaggaag gaaggactgc agctcttctt
    11641 cagaagatga aaagtgggga tgccagccag tttccaatcg aaacagctct caaggtactg
    11701 acaatcgaag gggctaaggt ccttggaatg gaaaatcaga taggaagtct ggaagtcggc
    11761 aagcaagcag attttctggt cattcaacca caagggaaaa ttcatctcca acctcaggaa
    11821 aatatgctgt ctcacctggt ttatgcagtt aaatctagtg atgtagatga tgtttatatc
    11881 gccggagaac aggttgttaa gcaaggtcaa gtcctgacag tagaacttta aaagaaaaat
    11941 cacgaaaaat tttaaaaaaa gtttgcaaaa atcttgcatt ctttttttga ctatgctata
    12001 cttatatacg gtttaaaaaa actgcctaag acagtagggg agctcgactc ataaatatcc
    12061 taccgaggac aagacgtatc atgtaaaaag aagcgtattg tactttcgtg tctaggtttg
    12121 ggcgcgtttt tctttttgaa aaattcccca agcaaaataa ttacggaggt gaacacacta
    12181 atgagtgaag caattattgc taaaaaagcg gaactagttg acgtagtagc tgaaaaaatg
    12241 aaagctgctg catctatcgt cgttgtagac gctcgtggtt tgacagttga gcaagataca
    12301 gttcttcgtc gtgagcttcg tggaagcgaa gttgagtata aagttattaa aaactcaatc
    12361 ttgcgtcgtg cagctgaaaa agctggtctt gaagatcttg catctgtatt tgttggacca
    12421 tctgcagtag cattttctaa tgaagatgtt atcgcaccag cgaaaatctt gaacgacttt
    12481 tctaaaaacg ctgaagcact tgaaattaaa ggtggtgcaa tcgaaggcgc tgtcgcatct
    12541 aaagaagaga ttcttgcact tgcaactctt ccaaaccgcg aaggacttct ttctatgctc
    12601 ctttctgtac ttcaagcgcc agtgcgcaac gttgctcttg cagtcaaagc ggttgcagaa
    12661 agcaaagaag acgcggctta atcttaagct acacagcgta gtctagctac gaaaaaaact
    12721 attataaaat ttaaaactta tttggaggaa ataacaatgg cattgaacat tgaaaacatt
    12781 attgctgaaa ttaaagaagc ttcaatcctt gaattgaacg accttgtaaa agctatcgaa
    12841 gaagaatttg gtgtaactgc agctgctcct gtagctgttg ctgcagctga tgcagctgat
    12901 gctggtgctg ctaaagattc attcgacgtt gaattgacat ctgcaggcga caaaaaagtt
    12961 ggcgttatca aagttgtacg tgaaatcact ggtcttggtc ttaaagaagc taaagaactt
    13021 gttgacggtg caccagcact tgttaaagaa ggcgttgcaa ctgcagaagc tgaagaaatc
    13081 aaagctaaat tggaagaagc tggagcttca gttactctta aataatagag tatcgcaatt
    13141 tgattgcgga atccctattt accagtcttt gagagcgata agcgattgat agaaactgat
    13201 aaattgattt ggtttcctgc caaaaattct ttggcaggat ttttgtttta ataaactttc
    13261 gggggtagac actagattgt ctaccccttt ttttgtgttt cggaggtaat tatgcaaaag
    13321 gaaggacagt catcacgtca attcgtgaag agtcatctca tgactatcat gggagttgat
    13381 attgtaaaag caactcagtt agttgatgaa atggaacaag taggtttaat ccgatttgat
    13441 gaatttggaa atgttggaat tttagtcttg gagggacaat aatgaagcgt attaccgcta
    13501 atcaatatca gacatcagag cgttattata agctccccaa acttttattt gagagtgaac
    13561 ggtataagaa tatgaaactg gaggttaagg tggtttactc agttttaaaa gatcggttgg
    13621 agttgtcttt gagcaaaggt tggattgatg aggatggggc tatttatttg atttattcca
    13681 attcaaattt gatggcactt ttaggctgtt caaagtcaaa attactctcc atgtgagttt
    13741 gaagtaacat ttttagatga ttaccataaa aaacacaact acccactatt ttacgagtcc
    13801 tatcttcaaa acgttatgga attccttgaa agtcaagaca taaagaatgg ggttgatgcc
    13861 tttgtagatg atcatcaaaa tctcgttttt gttttatatg gacaaggcta tcgagccgag
    13921 ggaaaagagg gaatacttac aacccaagta actgtaaaag cttatgatga agacaagaaa
    13981 ccgattaact tcgcaaattt attagattcc ttaatcgtct cagaatatca aatggaaccg
    14041 aatctttggg aggtctccca tgattgatct ctatctaagt aaaaatagac aaagaaatca
    14101 acttctttta gatttcttcc aaaactatgg catagaggta tcttgtcatt cagtttctga
    14161 aatgacaaag gacaaattaa ttgagatgat gagctattct tcagattgtt ttgagttttt
    14221 atctcccaat ttattacgat ttaagaaccg tgacaatcta agattaacgg atttcgttga
    14281 gataatttta aaaaatcctg aactaaccat cagacttcct cttgcggttt caaataagcg
    14341 agtttatcca agtctgaatt tggaagaggc tagagctcta ttgccaagag atacgaaaca
    14401 attgatttac atggcgcaaa cacattattt atctaactaa aggagaagca atatggctga
    14461 acgattttgg gagaacttgt ccattatttt ggctgaaaga aatatcagct gtattgagtt
    14521 aaccagaaaa atgtttgcgg gagagtttca ctatccaagt gaattgaatc gtttgtatca
    14581 aaagattcgc cactagaaga tggaacaacg aatgcctcaa agttcatggg tagaaaggat
    14641 tgtgcaggta ttagatttag attatgaaga tttatttcgg aggtaactat gaaaagaata
    14701 attccagttt atatattcca acaagtaaat gtcctattgg tatctctata cttattgaaa
    14761 tttctttgta tcggtgagtt aactatacta cagattctct atggttcgtc actcatttct
    14821 tttttatgga tgtatggcca acgcaaacaa gctcataagg taaatatgaa atctaggatg
    14881 aaatggcttg gtattggatt cgttagccta ctgattataa gtctatgttt tagtctaatc
    14941 catgcgcaag aaagcacgaa tcaagcaaac ttaattgggc ttcaacatca agttccttgg
    15001 ttttcatttt tattgttttt aatcaatgcg agtatggttg aagaatttct gtatagagaa
    15061 attttatgga acttggttag aaaattagat attcgagttg ctttgacaag tgttttattt
    15121 gccttagcac atcatccagg aaccattata gcttggtgtt tgtatgtatc gctggggatg
    15181 tttttagggc ttgtacgcta caaattggac ttatggggca gtatggggct acatttggtg
    15241 tggaacctac tagtttatag tttgctgctt ttttaaaata ggtgacctga tttatctcat
    15301 aaatctgtag tttttatggg attc
//
LOCUS       DAVKJD010000041        14628 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKJD010000041 DAVKJD010000000
VERSION     DAVKJD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14628
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.041"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            94..390
                     /locus_tag="VW157_001300"
     CDS             94..390
                     /locus_tag="VW157_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9552594.1"
                     /translation="MDTPDENGYVADNYRITYLEAHIKAMRDAIYQDGVDLLGYTTWG
                     CIDLVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIDSNGASIG"
     gene            511..1455
                     /gene="manA"
                     /locus_tag="VW157_001301"
     CDS             511..1455
                     /gene="manA"
                     /locus_tag="VW157_001301"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9552595.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1446..2950)
                     /locus_tag="VW157_001302"
                     /pseudo
     CDS             complement(1446..2950)
                     /locus_tag="VW157_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3055..3804
                     /locus_tag="VW157_001303"
     CDS             3055..3804
                     /locus_tag="VW157_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9552596.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGTAQYTSDAIAAYVQTNAEKIG"
     gene            complement(3824..4279)
                     /locus_tag="VW157_001304"
     CDS             complement(3824..4279)
                     /locus_tag="VW157_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9552597.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4299..5474)
                     /locus_tag="VW157_001305"
     CDS             complement(4299..5474)
                     /locus_tag="VW157_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9552598.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDNQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5533..6378)
                     /locus_tag="VW157_001306"
     CDS             complement(5533..6378)
                     /locus_tag="VW157_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9552599.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6503..7060
                     /locus_tag="VW157_001307"
     CDS             6503..7060
                     /locus_tag="VW157_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9552600.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7079..7546
                     /locus_tag="VW157_001308"
     CDS             7079..7546
                     /locus_tag="VW157_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9552601.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7635..8264
                     /gene="upp"
                     /locus_tag="VW157_001309"
     CDS             7635..8264
                     /gene="upp"
                     /locus_tag="VW157_001309"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9552602.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8438..9028
                     /gene="clpP"
                     /locus_tag="VW157_001310"
     CDS             8438..9028
                     /gene="clpP"
                     /locus_tag="VW157_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9552603.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9107..9355
                     /locus_tag="VW157_001311"
     CDS             9107..9355
                     /locus_tag="VW157_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9552604.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9456..10616
                     /locus_tag="VW157_001312"
     CDS             9456..10616
                     /locus_tag="VW157_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9552605.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            10884..11753
                     /locus_tag="VW157_001313"
     CDS             10884..11753
                     /locus_tag="VW157_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9552606.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGNNVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11757..12713
                     /locus_tag="VW157_001314"
     CDS             11757..12713
                     /locus_tag="VW157_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9552607.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12713..13477
                     /locus_tag="VW157_001315"
     CDS             12713..13477
                     /locus_tag="VW157_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552608.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13477..14187
                     /locus_tag="VW157_001316"
     CDS             13477..14187
                     /locus_tag="VW157_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552609.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
BASE COUNT         4477 a         2844 c         3136 g         4171 t
ORIGIN      
        1 gtttggattt cttctttagt tgatttcata tcactattat ataatgcttt tttattttag
       61 tttagtatta tcgtagaaaa tggactcggt gctatggata caccggatga gaatggttat
      121 gtagcagata actatcggat tacttactta gaggcccaca tcaaggccat gcgagatgcc
      181 atttaccaag acggggttga cttgcttggt tatacgactt ggggctgtat cgatctggtt
      241 tcagctggca caggggaaat gaataagcgc tatggcttta tctatgtaga tcgagataat
      301 gtaggaaacg gagctctcaa acgtagcaag aagaagtcct tctattggta caaggatgtc
      361 attgatagca atggtgcaag catcgggtag agcacatttg tttactattt ttataaaatc
      421 ttctccctgc tttataagat gtaaaaaaga gttcaattcg aactcttttt ttgctataat
      481 gaggggagaa aaatcagaca ggagatcgac atgtcagaac cattattttt acaatcagtt
      541 atgcaagaaa aaatctgggg tggagccaag ctacgtgatg agtttggcta cgacatccca
      601 agtgaaaaaa tcggagaata ttgggccatc tcagcccatc caaatggagt ctctaaagtt
      661 gccaatggtc gttacgaggg aacagatctt gctactttgt atgcggaaca ccgtgaatta
      721 tttggcaatc gtccagaacc tgtatttcca cttttgacca agatcctcga tgccaacgac
      781 tggctcagtg tccaagttca cccagacgat gcttatggac tcgagcatga aggcgaactc
      841 ggaaaaacag aatgctggta cattatcgca gcggatgaag gttcagagat tatctacggt
      901 cacaatgcca agtcaaaaga agaactccgc cagcaaatcg aggataagaa ctgggatgcg
      961 ctgttgacaa aagtgccagt taaagcagga gatttcttct atgtaccaag cggtaccatg
     1021 cacgctatcg gggcgggtat cttgattctt gaaacccagc agtctagcga taccacttac
     1081 cgtgtctatg actttgaccg taaggatgac aagggcaatt tacgtgaact tcaccttgaa
     1141 aaatctatcg atgtcttgaa cattggtgaa cccgcaaata gccgtccagt gacaatcaaa
     1201 gcagatgatc tacgttccac tctccttgta tccaatgatt tcttcgcagt ttacaaatgg
     1261 gaaattactg gaaaagttaa ctttgaaaag acagctgact acagcttgtt gagtgtcttg
     1321 gctggtcaag gtcaactaac tgttgacggg aaaaactatc caatccaaaa agggagacac
     1381 tttatcctac caagtgatgt tgaagcttgg actctggaag ggcaagattt agaattaatt
     1441 gttagtcatc cataaaaaag aaagggcctg agttcattac tcaagtcttt ttgattacat
     1501 aaattgggcg atgaagacca cgatgatgat tggaatgacg gaacgaagaa acgaagaagg
     1561 aagagccaga cttggaacag tccttgtttc catgctcttt catcgagatg gagttcctcc
     1621 attgcaagag cctttttaaa gatatatcct gtaaaaagtg aaaggtagag agctccaaat
     1681 ggcatgagga gattggaaac caagaagtcc atagcgtcaa agaaggtctt accaaaaatg
     1741 tgaacatccg ccatgacacc gtaagatagg gctgaaggaa tgccaaagac aaaggtcaaa
     1801 attcctaaaa taacactcca tttggcacgt ttgctgttat cctggttggt gatattgccc
     1861 acattgatct ccagcatcac gacagaagaa gtgacagtcg caaagaggaa gaggacgtag
     1921 aaaatggttc caaaaggcat cttgtcaaag agttgaggca agacgataaa gagcaagctc
     1981 ggtccccctt cagactggat attgaagggg gatcgagctt ggaaaatggc tagacctgcc
     2041 atgatggata tcgagatatt catggctacg atggagattc ctgactggac tagattggtt
     2101 ttcttgtcca agtaagaagc atatgtcaac atgactgtaa cccctagtga gagggcaaag
     2161 aaagattgtc ccagagcata gaggagacca gcgctagtca gttttgaaaa gtctggtttg
     2221 aggaagtaaa gaaccccttc catggcattt ggcaaactga tagagcgacc gatgataaca
     2281 acaaagatga taaagagcag gggcatcatg actttcgaag ctctttcaat ccctttttga
     2341 accccacgtg atacaatgaa gatattcaat aggataaagg ccgcttgagc tcctagggca
     2401 atggctggat ttgaaatgat tgaagtaaat aactgagtat aatcacccgt tccaccaagt
     2461 tggaacaatt tcccaaactc aatacctaga tagactagaa tccatcctcc gataacactg
     2521 taaaaagata agaggataaa gagggcaaag gcgccaatcc aaccgataaa gttgtacttg
     2581 ttattcttgc ccagttttcc aaaggttttg atagcggaaa cgccagcact acggccaagg
     2641 gcaaactcag cctgcaggag agggaaaccg attaaaatag tggaaatgag aaagattagt
     2701 aaaaagcctc caccgccatt agcagcagtc atgtagggaa acttccaaac ggaaccaagc
     2761 ccgatggcca gccagcagat gctagaataa aaccaagttt cgacccccat tgtgattttt
     2821 ctgacatagt taaactctga tctccttttt caaaataaga aaaagctact tgagttgtca
     2881 aatagccttc tgtttgattg atagacttga ctatcctatc atgttttcta aggtctgtca
     2941 agaaaatcat tttcaagttt tcacaccttt ctcaaaaaag ttaaaaaatt ttctcaaaaa
     3001 cgcttgactc tgacctaagg ggaggggtta tactatcatt gtaaggagga aatcatgtac
     3061 catataaaag aagctgcgca gctttcaggt gtctctgtca agaccctgca tcactatgac
     3121 aagataggac tcttggtccc cttaaagtcg gaaaacggct atcgaaccta cagtcaagag
     3181 gatttggaac gccttcaggt cattctttac tacaaatatc taggcttttc tttagagaaa
     3241 atagcagagc tgttaaagga agaaaggaca gatttattgc cccatttgac taggcagttg
     3301 gactatctaa ctcgcgaaag gcaacatctg gataccttga tttccacctt gcaaaaaact
     3361 attcaagaac aaaaaggaga aagaaaaatg accattgagg aaaaattcac gggatttagc
     3421 tatcaagaca atcaaaaata ccaccaagaa gcggtagaga aatatggtca agaagtcatg
     3481 ggacaagcgc tcgaacgcca aaaaggtcac gaagacgagg ctacggccgc ctttaaccaa
     3541 gtctttcaaa ctttggcaca aaatcttcaa gttggtttac ctgcaacagc aaccgaaaac
     3601 caagagcaag cagccaagct cttgcaagcc attcgcactt atggatttga ctgctctatt
     3661 gaggtattcg gtcatatcgg taaaggttac gtctacaacc cagagtttaa ggaaaacatt
     3721 gacaagtttg gttctggaac agcccaatac acgtcagatg ccattgcagc ttacgttcag
     3781 acaaatgcag aaaaaatagg ctaggaattt cctagcctat tttttacttc aaatcataaa
     3841 gccagtcgtc accgtttttg tagtaaaaga attcactgag atcttcttct agaaacacac
     3901 gaagcatatc agacatatca tcggttgcaa gttttagatg agaaagattt tcaaagtcct
     3961 cccaccaaac tttcccttcg tctgaagact ggagttcacc agtaaagtgt tctgtcttgt
     4021 aaaaaaggac gacataacga taatccttgt cgtcatacca gtttttgata ccacagagtt
     4081 ggggtttgga aatgatcaga ccagtttctt ctttcacttc acgaatgaca gcatcgacaa
     4141 aggattcgcc acgttcaaca tgaccaccag gaaaagtaat gccaggccag tcgggactaa
     4201 ctcggtcttg gaccaggacc ttatctccgt ttttaatcat acacatgtta acaaattcga
     4261 ctgcctctct tctgttcatt cttcacaacc tttaatcttt aatcataatg cagacttccc
     4321 gccacccagc cggtacagag ggcagaagtg atgttaaagc cacccgtgtg ggcattgata
     4381 tccataactt cgcctgcaaa gtggaggcca ggtaccagct tactttcaag ggttttagga
     4441 ttgatttcct tgagactgac tccacccttg gtaacaaagg actttgcaag ggacattttt
     4501 ccagttacag gaattttaag ttctttaatg gactggacaa gctgttctcg ttccttttca
     4561 gtcagctgtt tgactttttc aggatatcct tgtacaaaaa attcggccaa gcgttctggt
     4621 aacaaggttt ttaaagcgtt tttcaaggat ttttcccgat tttcttctag aaatgtaacc
     4681 aagtccttct cagaaagttg aggcaaaaca tcgagtgaga gaacctcccc acctttgaca
     4741 aagctagaca tgcgtagggc agcaggacct gacaaaccaa agtgggtaaa gagtaaatca
     4801 tgagtgatga catgcttacc ataacttagg gtcacatcgt ccaaagaaat cccttgtaag
     4861 gctttatgtg gaaaatctgt taataaagga ctttcagcag cctcaagatc ggtgatggta
     4921 tgcttaaaat ggcgagcaat ctcgtgacca aaaccagtcg aaccagtcga aggataagac
     4981 ttaccacctg ttgtgacaat gagtttctca caagtgaagg tttgatccgc tgacttaagg
     5041 acaaactggt tatctacttt tttaacagaa acgatttcta tttgagtagc aacttgacca
     5101 cctagttcgg tgattttctt ttccaaagct tcgataatag tccgagactt gtcactggct
     5161 ggaaagacgc gtccgtggtc ttcgacctta agtttaacac cattttctgt aaaaaagttg
     5221 atgatgtcat gattatcgaa ctgggagaaa acactgtaaa gaaagcgtcc gtttccagga
     5281 attccagcta gcaggttgtc taagctacca ttgttggtca cattgcaacg tcccccacca
     5341 gtcccagcta atttttttcc aagtttccga tttttttcga tgaggagggt tttctgtcca
     5401 taaaagctac tggaaatcgt agccatcata ccagcaggtc ccccaccgat gacaatagta
     5461 tcaaaatgtt tcatagctct attgtaccac aaaaaaacaa gagatgatgg tcacctcttg
     5521 tcaagaatgc aattaatcaa tttcatagcc catcagcaaa ccgccctctt ctgcatagaa
     5581 actgcagaga ccagaggttg gtagaatttt aatatccgct tgtgggaagg tttcacggat
     5641 tcgctctgag agctgttgac aacatttttc gttattgcgt tgggccatga caatacggcc
     5701 accagcatat ccagctttta ctaactcatc ataggcagct tgaactgatt tctttgatcc
     5761 ccttgctttt tgtagcaatt cgagagttcc agtttcacta gcttttccga ccatacgaat
     5821 gttgagaagg ccaacgaccg taccgataag cttgctcaaa cggccgttct tcaccaagtt
     5881 atcgactttg gctaggacaa agagcaactt agttttttct tgataggcgg tgatagcttc
     5941 aaccacttct tcaaaagaca agccctggtc aatcaagtca ttcaattttt ctacgagtag
     6001 gtcaacttca ccaccagcag ataaactatc aatcacatga atcttagtgt caggatggtc
     6061 ttccagataa atattctttg ctagttgagc actattgtga ctaccagaaa gggtacctgt
     6121 gatggttact aggaaaatgt ttttggcacc ttcaaatgct cgcaaatagt catctgggct
     6181 tggacaagcc gattttgaag cttctgcagt tgcatacatg gtttccatca tttggtcaat
     6241 atcgagactg gcgtcatcaa caaagacctg atcagctact tgaatggtta aggggacact
     6301 tacaaaggtt gtgttaatag ctggtgttgg cagttgacga taatcacaac cagagtcagc
     6361 aataatcttc caagtcatag aaattctcca tctttgtcag ttatacattg acaaaggttc
     6421 tgtctttttt tacaattata tcatgaaaac ccttgaaaca aaagcatcat ccgtctgttg
     6481 tgacaagtag aaagaaaatg ttatgtctga acgtagaatc tctgaaaagt ctcttgaaaa
     6541 tctcagaaaa tcaaaccaag aatccaattt attaaccaga gaagccattg aaacagccct
     6601 cttgcaactc ttggaaaaaa aggaactgac caagattagt atttctgaat tggtcaaacg
     6661 tgcaggtgtt tcgcgtgcgg ccttttatcg caattatgac tccaaagagg aaattttaga
     6721 gagcgtcttt aaacgaactg tccacaatat tatggaacag ctgcatcatt acgatttaaa
     6781 gacagacctt tatttggttt gggttcacct tttccgggag gccagaaagg aagctagagt
     6841 gattcaattg gccttggatt accatctgga aaaaatcttt gtccaagcca tgcaggaatt
     6901 tctagaaaaa taccatggga aatcaaaagg tgtcagctct tatcttcatt ccttctggag
     6961 ctcagccatc gtctctgtcc ttctaaaatg gatcaaggat ggcatgaagg taccagctga
     7021 aaagattgca gatttaggtt taccattttt taaaaaatag agaaaaagga gaaaagagat
     7081 gactgaaaaa agactggcct gggatgagta ttttgcagcc caagccctac taattgcgaa
     7141 tcgttccact tgtaaacgtg ctaaagtggg cgcgattctg gtaaaagata ataaggttat
     7201 ttccactggt tacaatggtt cggtgtcagg gactgagcat tgtattgatc acgaatgttt
     7261 ggtcattgaa ggccactgtg ttcgcaccct tcacgctgag gtcaatgcta tccttcaagg
     7321 tgctgagcgt ggtgttccca aaggctttac agcctatgta acccattttc cttgtctcaa
     7381 ctgtacaaaa caattgcttc aggtcggctg caagcgtgtg gtttatatca accagtaccg
     7441 aatggacgac tacgcccaat acctttatca agaaaaaggg acagaattga cccatttacc
     7501 acttgagaca gtacagacag ctcttaaaga ggctgatcta atgtaaaaat tatcaaaaat
     7561 aaatggttta gaaagatttt taaaccgttt tttggtataa taagaagaat aaattgaaag
     7621 aaggaattcc aaaaatggga aaaattgaag ttattaatca cccactgatt caacacaaat
     7681 tgtcaatctt gcgtcgtaca gatacttcta caaaagcttt tcgtgagcta gtagatgaga
     7741 ttgcaatgtt gatggggtat gaagtacttc gtgatcttcc actagaagat gtggaaatcg
     7801 aaacaccaat tacaaaaaca gttcaaaaac aattggcagg taagaaattg gccatcgtcc
     7861 caatcttgcg tgcaggtatc gggatggttg atggtctctt gagcttggtt ccagctgcta
     7921 aagttggcca catcggtatg taccgtgatg aagaaacact tcaaccagtt gaatacttgg
     7981 tgaaattgcc tgaggacatt gaccaacgtc aaatttttgt agtagaccca atgttggcaa
     8041 caggtggctc agcaatcttg gctgttgatt ctcttaaaaa acgtggcgca tcaaatatca
     8101 aatttgtctg ccttgtatct gctccagagg gtgtaaaagc ccttcaagaa gctcatccag
     8161 atgtagaaat ctttacagca gccttggatg aacgtttgaa cgaacacggt tatatcgttc
     8221 caggtcttgg agatgctgga gaccgcttgt tcggtacaaa ataagatcga aaagaagaat
     8281 gacttggaaa agtatttcta gtcacgaaag gaggttgggt ttttgtttct gtctaatgaa
     8341 agcagagcaa aaatttgacc ttttttgacc aagatattat aatagtctta tcttcagtca
     8401 tttgatcaac aaaattaaaa ctcaaaagga gaaatgaatg attcctgtag ttattgaaca
     8461 aacaagccgt ggagaacgtt cttacgatat ttactcacgt cttctcaaag accgcatcat
     8521 tatgctgaca ggtccggttg aagacaatat ggctaactct gttattgccc aattgctttt
     8581 cttggatgcc caagatagta caaaagatat ttacctttat gtcaatacac caggtggttc
     8641 tgtttcagct ggtttggcaa tcgtagatac catgaacttt atcaaggcag atgtccaaac
     8701 catcgttatg ggaatggctg catctatggg aacagtcatc gcatcaagtg gagcaaaagg
     8761 caaacgtttc atgcttccaa atgctgaata catgattcac caaccaatgg gcggtacagg
     8821 tggtggtacc caacaaactg atatggcgat cgctgcagaa cacttgctca aaactcgtaa
     8881 taccttggaa aaaatcttgg ctgaaaattc aggtcagtca atggaaaaag tccatgcaga
     8941 tgcagaacgt gataactgga tgagcgccca ggaaacactt gaatatggct ttattgatga
     9001 aattatggcc aacaattcat tgaactaatg atgatagaag gcaaactcga ctgggtttgc
     9061 tttttttggt ataataggga gagatttctt agaaagagga tttatcatgt ttgaaaaagt
     9121 caatcgctct ggcttgatta tctatcttta ctataatcgt gatgccaaaa aactgcagga
     9181 ttatggagat attacctatc attccaagaa acatcgttac ttacaactct atgttccaac
     9241 tcaagaagtg gagcaattgg tcggacgctt gagcaaggaa aaatttatta aaaaagttag
     9301 ggtttgtcat atccaagagt tggaaacacc ctttgtgggc aatctttatc gataggaaaa
     9361 cgttatcatc gaaaaagttt aaggaaaata ttgacaattt tctgataatt cggtatattc
     9421 ttaacagact atttaagaaa taaggagaca aaaagatgaa gaaaaaattt gccctatcgt
     9481 ttgtggcgct tgcaagtgta gcacttcttg cagcctgtgg agaagtgaag tctggagcag
     9541 tcaacactgc tggtaactca gtagaggaaa agacaattaa aatcgggttt aactttgaag
     9601 aatcaggttc tttagctgca tacggaacag ctgaacaaaa aggtgcccaa ttggctgttg
     9661 atgaaatcaa tgccgcaggt ggtatcgatg gaaaacaaat cgaagtagtc gataaagata
     9721 ataagtctga aacagctgag gctgcttcag ttacaactaa ccttgtaacc caatctaaag
     9781 tatcagcagt cgtaggacct gcgacatctg gtgcgactgc agctgcggta gcgaacgcta
     9841 caaaagcagg tgttccattg atctcaccaa gtgcgactca agatggattg actaaaggtc
     9901 aagattacct ctttattgga actttccaag atagcttcca aggaaaaatt atctcaaact
     9961 atgtttctga aaaattaaat gctaagaaag ttgttcttta cactgacaat gccagtgact
    10021 atgctaaagg gattgccaaa tctttccgcg agtcatacaa gggtgaaatc gttgcagatg
    10081 aaactttcgt agcaggtgac acagacttcc aagcagccct tacaaaaatg aaagggaaag
    10141 actttgatgc tatcgttgtt cctggttact ataatgaggc tggtaagatt gtaaaccaag
    10201 cgcgtggcat gggaattgac aaaccaatcg ttggtggtga tggattcaac ggtgaggagt
    10261 ttgtacaaca agcaactgct gaaaaagcat caaacatcta ctttatctca ggcttctcaa
    10321 ctactgtaga agtttcagct aaagctaaag ccttccttga cgcttaccgt gctaagtaca
    10381 atgaagagcc ttcaacattt gcagccttgg cttatgattc agttcacctt gtagcaaacg
    10441 cagcaaaagg tgctaaaaat tcaggtgaaa tcaaggataa ccttgctaaa acaaaagatt
    10501 ttgaaggtgt aactggtcaa acaagcttcg atgcagacca caacacagtc aaaactgctt
    10561 acatgatgac catgaacaat ggtaaagttg aagcagcaga agttgtaaaa ccataataga
    10621 aaaatgttga aatagggaat gagcctttga ctcactccct gtttcgatat ttaatactct
    10681 tcgaaaatct cttcaaactg cgtcaacgtc gccttggatt atatatgtga ctgacttcgt
    10741 cagtcttatc tacaacctca aagcagtgct ttgagcaacc tgcggctagt ttcctagttt
    10801 gctctttgat tttcattgag tataagaacc tatcaaaaag tgagggaaaa ccctcggaat
    10861 tataaataga aagagtgaat cttatgctcc aacaactcgt aaatggtttg attctaggta
    10921 gtgtttacgc gctgttagcc ctaggatata ccatggttta cggaattatc aagctcatca
    10981 acttcgccca tggtgatatt tatatgatgg gagcctttat cggttatttt ttgatcaatt
    11041 ctttccaaat gaatttcttt gtagcgctta ttgtagctat gctagcgaca gctattcttg
    11101 gtgtcgtgat tgagtttctt gcttaccgac ctttgcgcca ctctactcgt attgctgttt
    11161 tgattacggc tattggggtt tctttcctat tggagtatgg aatggtctat ctggttggtg
    11221 ccaatacccg tgccttccct caagcgattc aaacagttcg atatgatttg ggaccaatta
    11281 gcttaacaaa tgtgcagtta atgattttgg ctatttcctt gattttgatg attttgttac
    11341 aagtcattgt ccaaaagact aagatgggga aagccatgcg tgcagtatca gtagatagcg
    11401 acgcggcgca attgatgggg aacaatgtaa accgtacgat tagctttacc ttcgctttgg
    11461 gttctgctct tgcgggtgcg gctggtgttc tgattgctct ttattataac tctcttgagc
    11521 ctttgatggg ggttactcca ggtcttaaat ctttcgttgc cgcagtactt ggtggtatcg
    11581 gaattattcc tggtgcggct cttggtggct ttgtgattgg tctattggaa acctttgcga
    11641 ctgcctttgg gatgtcagat ttccgtgatg ccattgttta tggaatcttg ttgttgatct
    11701 tgattgtccg cccagctggt atccttggta agaatgtgaa agagaaggtg taaacgatga
    11761 aggaaaattt aaaagttaat attctgtggt tactcctttt gttagctggc tatagcttga
    11821 ttagtgtact ggtttcagtc ggagtactta atctattcta tgtacagatt ttacaacaaa
    11881 ttggaattaa tattattttg gctgttggtc tcaacttaat cgttggtttt tcaggacaat
    11941 tttcacttgg tcatgctggt ttcatggcga ttggtgccta tgcagcagct attattggtt
    12001 ctaaattacc aacctacggt gccttctttg gagctatgct tgtaggagct ttgctttcag
    12061 gagcagttgc cttacttgtc ggcattccaa ccttgcgctt gaagggggac tatcttgcgg
    12121 tagcaactct gggtgtttct gaaattatcc gtatctttat catcaatggt ggaagcctta
    12181 caaatggtgc ggcaggtatc ttagggattc ctaactttac aacttggcaa atggtttact
    12241 tctttgtcgt gattacaacc attgcaacct tgaacttctt gcgtagccca attggtcgtt
    12301 caaccctctc tgttcgtgaa gatgaaatcg ctgctgagtc agttggggtt aatacgacta
    12361 aaattaaaat catcgctttt gtctttggtg ccattactgc aagtattgct gggtcacttc
    12421 aggcaggatt tatcgggtct gttgtaccga aagattacac cttcatcaac tcaatcaacg
    12481 ttttgattat tgttgtattt ggtggactcg gttccattac aggtgcgatt gtttcggcta
    12541 ttgttctggg aattttgaat atgcttctcc aagatgttgc tagtgtgcgt atgattattt
    12601 acgctttggc cttggtattg gtaatgattt tcagaccagg tggactcctt ggaacatggg
    12661 aactgagcct atcacgtttc tttaaaaaat ctaagaagga ggaacaaaac taatggcatt
    12721 acttgaagta aaacagttaa ccaaacattt tggtggtcta acagctgttg gagatgtgac
    12781 tcttgaattg aacgaagggg aactggttgg attaatcggt ccaaacggag ctgggaaaac
    12841 cacccttttt aaccttttga ccggtgttta tgaaccaagc gagggaacag taaccctaga
    12901 tggtcacctt ttgaatggga aatcacctta taagattgcc tctttgggac ttggacgtac
    12961 tttccaaaat atccgtctct ttaaagattt aacagtttta gacaatgttt tgattgcttt
    13021 tggaaaccat cacaaacaac atgtttttac tagtttctta cgcttaccag ctttttacaa
    13081 gagtgaaaaa gaattaaagg ctaaagcttt ggaattgttg aaaatctttg atttagatgg
    13141 tgatgcagag actcttgcta aaaatctttc ctacggacaa caacgtcgtt tggaaattgt
    13201 tcgtgccctt gctacggaac ctaaaattct cttcttagat gaaccagcag caggtatgaa
    13261 cccacaggaa acagccgaat tgactgagtt aattcgtcgt atcaaagatg aatttaagat
    13321 tacaatcatg ttgattgaac acgatatgaa tctggtcatg gaagtaacag aacgtatcta
    13381 cgtacttgaa tatggccgtt taattgctca aggaactcca gacgaaatta agaccaataa
    13441 acgcgttatc gaagcttatc taggaggtga agcctaatgt ctatgttaaa agttgaaaat
    13501 ctttctgtgc attacggtat gatccaagca gtccgtgatg taagctttga agttaatgaa
    13561 ggagaagttg tttcccttat cggtgccaac ggtgcaggta agacaactat tcttcgcacc
    13621 ttgtcaggtt tggttcgacc aagttcagga aagattgaat ttttaggtca agaaatccaa
    13681 aaaatgccag ctcagaaaat tgtggcaggt ggtctttcac aagttccaga aggacgccac
    13741 gtctttcctg gcttgactgt tatggaaaat cttgaaatgg gagctttctt aaagaaaaat
    13801 cgtgaagaaa atcaagctaa cttgaagaag gttttctcac gctttcctcg tcttgaagaa
    13861 cgtaagaacc aagatgcagc tactctttca ggaggggaac aacaaatgct tgccatggga
    13921 cgcgctctta tgtcaacacc aaaacttctt cttttagatg aaccatcaat gggacttgcc
    13981 ccaatcttca tccaagagat ttttgatatc attcaagata ttcagaagca aggaacaacc
    14041 gtcctcttga ttgaacaaaa tgccaataaa gcacttgcaa tctctgaccg aggatatgta
    14101 ctggaaacag ggaaaatcgt cctatcagga acaggaaaag aactcgcctc atcagaagaa
    14161 gtcagaaaag catatctagg tggctaaaac aatccagtgg attgttttag tcggcagatg
    14221 aagattacga agtaatcatc attatagtct gggagactag tttaggttgc agataaagat
    14281 tgcgaagcaa tcatcaaatc cagtggattg ttttagtcgg cagatggaga ttacgaagta
    14341 atcatcaata tagtccgggg gaccttttta gtcggtagat tgagattgca aacaaatctg
    14401 catctacatt gaaagcttaa tttctaataa ttgaaaaaat cgaatgaaaa atttcttacc
    14461 ttcattcaca gagctcgatt tcagagctct ttttgctagc ttattcatac ttttctgaat
    14521 ttcgaaaaag aaatgtaagc gtttgataga tttacaaaaa gattgtataa tagggataag
    14581 aatagaaaag gagaagtctc atggcagtta accccaatta ttcacccc
//
LOCUS       DAVKJD010000042        14484 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKJD010000042 DAVKJD010000000
VERSION     DAVKJD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.042"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(191..907)
                     /locus_tag="VW157_001317"
     CDS             complement(191..907)
                     /locus_tag="VW157_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9552610.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            1256..3043
                     /locus_tag="VW157_001318"
     CDS             1256..3043
                     /locus_tag="VW157_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9552611.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPCEGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDSEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            3040..3516
                     /locus_tag="VW157_001319"
     CDS             3040..3516
                     /locus_tag="VW157_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9552612.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            3544..4449
                     /locus_tag="VW157_001320"
     CDS             3544..4449
                     /locus_tag="VW157_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9552613.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            4436..5251
                     /locus_tag="VW157_001321"
     CDS             4436..5251
                     /locus_tag="VW157_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9552614.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            5258..5662
                     /locus_tag="VW157_001322"
     CDS             5258..5662
                     /locus_tag="VW157_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9552615.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            5961..7127
                     /locus_tag="VW157_001323"
     CDS             5961..7127
                     /locus_tag="VW157_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9552616.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTAYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            7243..8280
                     /locus_tag="VW157_001324"
     CDS             7243..8280
                     /locus_tag="VW157_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9552617.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            8488..9342
                     /locus_tag="VW157_001325"
     CDS             8488..9342
                     /locus_tag="VW157_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9552618.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            9492..9749
                     /locus_tag="VW157_001326"
     CDS             9492..9749
                     /locus_tag="VW157_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU9552619.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(10214..10306)
                     /locus_tag="VW157_001327"
                     /pseudo
     CDS             complement(10214..10306)
                     /locus_tag="VW157_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(10342..10638)
                     /locus_tag="VW157_001328"
     CDS             complement(10342..10638)
                     /locus_tag="VW157_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9552620.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(10628..10789)
                     /locus_tag="VW157_001329"
     CDS             complement(10628..10789)
                     /locus_tag="VW157_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002875407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU9552621.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTVQNLDGAYSLYKMA
                     NPEGVEYGN"
     gene            10859..11623
                     /locus_tag="VW157_001330"
     CDS             10859..11623
                     /locus_tag="VW157_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9552622.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKAYPVYVVNYKDEEICLVQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIERVLD
                     DRGIPYEEVMTWSTDGFYRETAEKVAYRKEEDCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            12188..13567
                     /locus_tag="VW157_001331"
     CDS             12188..13567
                     /locus_tag="VW157_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9552623.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLITFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTLGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            13581..14246
                     /locus_tag="VW157_001332"
     CDS             13581..14246
                     /locus_tag="VW157_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9552624.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
BASE COUNT         4014 a         2703 c         3268 g         4499 t
ORIGIN      
        1 tataaaattt aaacttaaga tttcagattg gtcggaaaaa atacgtatat atatctagta
       61 taatttttgg ttctatttct ataaaatatt ccacaaatta tagaattttc caaaaatagg
      121 taagcgctac ctttttggtg tagtataata agcatagaaa aagcccaagc gattagctca
      181 ggttttcttc ttagtgatca cggtcacatg agataaattt aatcttgtag taatcagatc
      241 gtttgtaagt ttcactgtat tctaaaactt ggccagttga ttcgagtttg gtgattttag
      301 tttgtaggac agtagggaat tgttcatcga ctccgaggac tgaagctgca tgttctggag
      361 ttggaaagac tatttcgttg atttcttcaa agtgttcatc attcatgtga atgtggtagt
      421 ctaacttgaa acgattatag atagaactat agtattcaag gtttggataa tttgcgttga
      481 tatattgttc tgggatgtag gatgtatggt agatataaac gacaccgttt gattcgcgga
      541 tacgttcaat cttgtagtag aattgatcgc cgcgtagacc caatttttcc aagtaaacaa
      601 gcttgtttcc gcgttcaatt gaaagaacag ttaccttatc atctttagca ttgaagagtt
      661 caatatctga aaactctaca agcttgtgtt tgcgtgcacg tgaaacgaag gttccttttc
      721 cttgttggcg gacaatatag ccatctttgg caaggtcgtt taaggcgcga acaactgtga
      781 tagagctgac atcgtacatt gaaatgagtt ctgcttcagt gtaaaattta tctccactgc
      841 taaactgccc agagatgatt ttatttttta attcgtcttt tatgtattga tatttgggaa
      901 ttgccatatt tttacctcta ttttccttct tccatagaaa attttaccat aaaagcgaaa
      961 aaaatagtag tcttttgaat aaaaaattag aataatagtt tgaaaaatta agcgatatag
     1021 ggattgctca tattttataa atgtattcta tacaaaatta ttttaggtcc tatattccga
     1081 atttaaagag cgtttgaaat gggtctaaaa atgtttgaaa ctaattgaat gtgtgtataa
     1141 tagtaaaaat gcagtaaata tcccgacaat ttctattgac aattcaaaca gattggttta
     1201 taattaatat aacaacaaat gaaagcgcaa actttcgcgg tcggaaggta gttttatgac
     1261 acgatttgag atacgagatg atttctatct cgatggaaaa tcatttaaga ttttatctgg
     1321 tgccattcat tattttaggg ttcctccaga ggattggtat cattcgctct ataacttgaa
     1381 ggctcttggt tttaatacgg tagagactta tgttgcttgg aatttacacg agccttgtga
     1441 aggtgagttt cattttgaag gtgatctgga tttagagaaa tttctccaaa tagcgcagga
     1501 tttgggtctc tacgcaattg tgcgtccgtc tccatttatc tgtgcggaat gggaattcgg
     1561 tggcttacca gcttggctct tgaccaagaa catgcgaatt cgctcatccg acccagcata
     1621 tatcgaggca gttggtcgct actatgatca gttattgcca agactggtgc ctcgtttgtt
     1681 ggacaatggt ggcaatattc tcatgatgca ggttgaaaat gagtatggtt cttacggaga
     1741 agataaggct tacctgagag cgattcgaca gctaatggaa gagtgtggcg taacctgtcc
     1801 cctctttaca tcagatggtc catggcgagc tactctgaaa gctggaacct taattgaaga
     1861 ggacctcttt gtaacaggaa actttggttc taaggcacct tacaactttt cgcagatgca
     1921 ggaattcttt gatgaacatg gtaagaaatg gccactcatg tgtatggagt tctgggatgg
     1981 ttggttcaat cgctggaaag agccgattat cacacgggat cctaaggaat tggcagatgc
     2041 agttcgagag gttttggaac aaggctctat caatctttac atgttccacg gtggtacaaa
     2101 ctttggtttc atgaatggtt gctcagctcg aggaactttg gacctgccac aagttacatc
     2161 ttatgattac gatgcccttc tggatgaaga aggaaatcca actgctaaat atcttgcagt
     2221 caagaagatg atggcaacac atttttcaga gtatccgcag ttggaaccac tctacaaaga
     2281 gagtatggag ttggatgcta ttccactagt tgaaaaagtt tctttgtttg aaaccttaga
     2341 tagcttgtca agtcctgtag aaagtctcta tcctcaaaag atggaggaac tgggacaaag
     2401 ttatggctac ctactttatc gaacagaaac aaactgggat tcagaagaag aaagacttcg
     2461 tatcattgat ggtcgagata gggcccagct gtatgtcgat ggtcagtggg ttaaaactca
     2521 atatcagaca gagattgggg aagatatttt ttatcaaggt aaaaagaaag ggctatctag
     2581 gttagatatc ttgatagaaa atatggggcg tgtcaactat gggcataagt tcttagcgga
     2641 tacgcaacgt aagggaattc ggacaggggt ctgtaaggat ctgcatttct tactaaactg
     2701 gaaacactat ccactcccac tagacaatcc tgagaaaatt gatttttcaa aaggatggac
     2761 tcaaggacaa ccagcctttt acgcttatga ctttacagtc gaagagccaa aagatactta
     2821 cctagacttg tctgagtttg gtaagggagt tgcctttgtc aatgggcaga atctaggacg
     2881 tttttggaac gttggcccaa ctctctcact ttatatccct catagctatc tcaaggaagg
     2941 tgccaaccgt atcattatct ttgaaacaga aggtcaatat aaagaagaga ttcatttaac
     3001 tcgtaaacct acactaaaac atataaaggg ggaaaactta tgacaattgt aggatgccgt
     3061 attgatggac gtttgatcca cggacaagta gccaatcttt gggctggaaa actaaatgtt
     3121 tcacgcatta tggttgtaga cgacgaagtt gtcaacaacg atattgaaaa gagtggtttg
     3181 aaacttgcga caccaccagg tgtgaaattg agtattttgc cagttgagaa agctgcagcc
     3241 aatattcttg ctggtaaata cgatagccaa cgtctcttta tcgtggctcg taaaccagac
     3301 cgcttccttg gtttggtaga agcaggtgta ccacttgaaa cccttaatgt tgggaatatg
     3361 tctcaaacac cagaaactcg ttctattaca cgttctatca acgtagtaga caaggatgtg
     3421 gaagacttcc acaaactggc agaaaaaggt gttaaactta ctgctcagat ggttccaaat
     3481 gatccaattt cagacttttt gagcttatta aaataggaaa aaaattttta ggaggtcatt
     3541 gttatgatac aatggtggca aattttactt ctcactttgt actcagctta tcaaatctgt
     3601 gatgagttga cgatcgtttc atctgcaggt tcccctgtat ttgctggttt cattactggt
     3661 ttaatcatgg gagatgtgac tactggttta cttatcggtg gtaacttgca actgttcgtt
     3721 cttggggttg gtaccttcgg tggtgcttct cgtatcgacg caacttctgg tgcggttctt
     3781 gcgacagcct tctctgtttc acaaggaatt gatgcaccgc ttgccattac tacaatcgct
     3841 gtaccagtag cagctctctt gacttacttc gacgttcttg gtcgtatgac tactaccttc
     3901 ttcgctcacc gtgtggatgc tgcaatcgaa cgctttgact ataaaggtat tgaacgcaac
     3961 tacttgcttg gtgcgattcc gtgggctcta tctcgtgccc ttccagtctt ctttgccctt
     4021 gcttttggtg gtgcctttgt acaatcagta gtagacttcg ttgaagccta caaatgggtt
     4081 gcagatggct tgacactcgc aggacgtatg cttccaggtc ttggatttgc aatcttgctt
     4141 cgttaccttc cagttaaacg taaccttcac taccttgcta tgggatttgg tttgacagct
     4201 atgttgactg ttctttactc atatgtaaca ggtcttggtg gcgctgttgc tggtatcgta
     4261 ggtactcttc ctgctgaagt tgctgaaaaa attggtttcg tgaacaactt caaaggtttg
     4321 tctatgattg gtatttctat cgtaggtatt ttccttgcag tgcttcactt caaaaatagc
     4381 caaaaagtag ctgtagcagc accttctaca ccatcagaaa gtggggaaat cgaagatgac
     4441 gaattctaat tacaaactta caaaagaaga ttttaatcaa atcaacaaac gtagcttgtt
     4501 tactttccaa ttaggttgga actacgaacg tatgcaagct tctggttacc tttacatgat
     4561 cttgcctcag ttgcgtaaaa tgtatggtga tggaactcct gaattgaaag aaatgatgaa
     4621 agttcatact caattcttca atacttcgcc attcttccat accattatcg ctggttttga
     4681 ccttgccatg gaagaaaaag atggtgtagg ttcaaaagac gccgttaacg gtatcaagac
     4741 aggtttgatg ggaccattcg ctcctcttgg ggatacaatc tttggttcac ttgtacctgc
     4801 tatcatgggg tcagtcgcag caactatggc tatcgctggc caaccttggg ggatcttcct
     4861 ttggattgca gttgcagtag cgtatgacat cttccgttgg aaacagttgg aatttgctta
     4921 caaagaaggg gttaacctta tcaacaacat gcaaagtacc ttgacagctt tgattgacgc
     4981 tgcatctgta cttggtgtct tcatgatggg tgctcttgta gcaacagtga ttaactttga
     5041 aatttcttac aagttgccaa tcggtgaaaa gatgattgat ttccaagaca tattgaacca
     5101 aatcttccca cgtttgcttc cagcaatctt tactgccttt atcttctggt tgcttggtaa
     5161 gaaaggtatg aactctacta aagctatcgg tattattatc gtacttgctt tggctctttc
     5221 tgcccttggt cactttgcac ttggaatgta attccttatg actaaatcat taattttggt
     5281 gagccatggt cgcttctgtg aggagcttag aggtagcaca gaaatgatta tgggcccaca
     5341 agacaacatt tacacagtag ctcttcttcc agaagatggc ccagaagaat ttactgctaa
     5401 atttgaagct gttattgaag gattggatga tttcctagtt tttgcggatc ttctcggtgg
     5461 gacaccttgt aatgtggtga gtcgcttgat catggaaggt cgtgatattg acctttacgc
     5521 agggatgaat cttccaatgg tgattgaatt tatcaatgcg agccttacag gcgcagatgc
     5581 ggactacaag agccgtgctg cagaaagcat tgtgaaagtt aatgacctgt tagcgggctt
     5641 cgatgatgac gaagatgaat aatactcttc gaaaatctct tcaaactacg tcaacgtcgc
     5701 cttgccgtag gtatatgtta ctgacttcgt cagtcttatc cggcaacctc aaaacggtgt
     5761 tttgagctga cttcgtcagt cttatccggc aacctcaaag cagtgctttg agcagcctgc
     5821 ggctagtttc ctacagattt tagttggaac tcgattcaat tcatgtgaca acgtgaaaat
     5881 cgttagagca ttttatatag aatatacatg ggaatggagc ttactcccat tcccatattt
     5941 aatagaaaaa gaggaactca atgctacatt atacaaaaga agacttgctc gaattgggtg
     6001 cagaaatcac tacgcgtgaa atctaccaac agcctgatgt atggagagaa gcttttgaat
     6061 tttatcaagc aaaacgtgaa gaaattgcag cctttctaca agaaatcgct gataaacatg
     6121 actatattaa ggttatcttg acaggtgctg ggacttctgc ttatgtggga gataccttgc
     6181 taccttactt taaggaagtc tatgacgaac gcaaatggaa tttcaatgct attgcgacaa
     6241 cagatatcgt tgccaaacca gcaacctatt tgaaaaaaga tgtggcaact gtccttgtgt
     6301 cttttgctcg tagtgggaac tcgcctgaaa gtgtggcgac tgttgatttg gccaaatcct
     6361 tggtggatga gctttatcaa gtgacgatta cttgtgcagc agatggtaaa ttggctcttc
     6421 aagctcacgg tgatgaccgt aatctcttgc tcttgcaacc agctgtctct aatgatgctg
     6481 gatttgccat gacttctagc tttacgtcta tgatgttgac agctctcttg gtctttgatc
     6541 ctacagaatt tgctgttaag tctgaacgtt ttgaagttgt atctagtctt gcccgtaaag
     6601 ttttagacaa ggtagaagat gtcaaagagc tcgttgattt agactttaac cgtgtcatct
     6661 atctaggcgc tggtcctttc tttggacttg ctcatgaagc tcagctcaag attttggaat
     6721 taactgctgg tcaagttgcg accatgtatg aaagcccagt tggcttccgt cacggtccaa
     6781 aatctcttat caacgacaat acagttgttt tggtctttgg tacaacgaca gcctacactc
     6841 gtaagtacga cttggacttg gttcgtgaag ttgctggtga ccagattgct cgtcgtgttg
     6901 tgcttttgag tgatcaagct tttggtcttg aaaatgtcaa agaagtggcc cttggttgtg
     6961 gcggtgtctt gaatgatatt taccgtgtct tcccttacat cgtttatgcc caactctttg
     7021 ccctattgac ttcactcaag gtagaaaata aaccagatac accgtctcct acaggtacag
     7081 taaaccgtgt agtacaaggt gtcataattc acgaatatca aaagtaagac agtgtttatg
     7141 aattcttgac aagaggattt gtaaattatc agataaacca tagattgtca gtacgctttc
     7201 tatggtttgt ttgcttgaga gaaatagtaa aaggagaaca gaatgaaagc atacacagag
     7261 cgtgtatttg gaaatgttga gggcgaggat gtcttggcct atcgatttga gacagacggt
     7321 ggctaccaac ttgaggttat gacttatggt gcgaccatct tgcgctatgt cgcacctgac
     7381 aaggctggaa attttgccaa tgttatcttg ggatttgatg actttgatag ttatgtaggc
     7441 aatagtccca agcatggagc aagtgtaggt cctgtagcgg gtcgtattgc aggtgcgacc
     7501 tttgagctca atggtaagac ctatgacctt gaggttaata atgctagcaa ctgtaatcac
     7561 agtggttcaa ctggttggga ttccagcttg tttgaagttg aagaagtaag cgatcatggc
     7621 ttgactctct acacagagcg tacagatggg acaggagggt tccctggaaa tctcaagatt
     7681 tggattagtt atcacttgga agaaactggt gcctatgaaa tcagctacaa ggtaacgacc
     7741 gatcaggata cgctggtcaa tccaaccaac cacagctatt tcaacttgtc tggtgatttc
     7801 acgcagacga ttgaccgtca tgtcttccaa ctaaacacag agggcattta ctcaatcgct
     7861 cctgacggtg ttcctgccaa aactccagaa gccaaccgtg atgtggtcaa acacatctac
     7921 aatggtgcct tgttgaagga tatctttgca gaagaagatg agcaaatcca gctggcatca
     7981 ggtttggatc atccatttgc ccttcctgca ggccatgaca atgctggatt cctttatgac
     8041 caaaattcag gtcgcttcct gcttttcaag acagaagctc cttgctttgt ggtctacaca
     8101 gcaaactttg tggatgaaag tgtcatcata ggaggtcagc caatgctaca gcacaatggg
     8161 attgcccttg aagcgcaagc tttaccagat gccattcaca gtgaccttaa aggtcaagtc
     8221 attcttaaag ctggtcaaac cttcaccagt aagacacgtt atgaacttgt tgtgaaataa
     8281 aaaaagctgg tcaaaaagtc tttaaaatta aaataacgca tattgtcagg tgatgaaaag
     8341 tttgataata tgcgttttat tatgaaatga tttactttat cttatcctga aatggtgtta
     8401 tttttgagtt atctggcttg actttcttga tggaaattat ataatagttg taataacaat
     8461 aattacaacg agaaaagagg accatttatg acctatgaat acaagagtca catttatttg
     8521 gcagagacag ttttaaatgt aaaggatttg gccagtcaaa cagcctttta tcagcaagtt
     8581 attggtttag aaatcttatc tcaaactgag acagagtcca ttctaggcct tggtggaaaa
     8641 gtcttggtac agctgattca agcacaagag agcggagaag tgagggaaca ttatggtctt
     8701 taccatttgg ccattctctt gcccacacgc aaggctttgg ctgatgtctt gaaacacctg
     8761 acagatttac agattcctct tgtcggcggt gcagaccatg gttacagtga agctatttac
     8821 ttggaagatt tggagggaaa tggcattgaa ctctatcgag ataagccagt ttccacatgg
     8881 gatattcgag aagatggacg cattatcgga gtgacagagg ctcttgcggc gcaggacatt
     8941 tatgagttgg gggaaagagt agagcccttt atcctggcag aaggtacgag aatggggcat
     9001 attcatcttt ccgtcaagga tagtcgaaag tccagccagt tttatcaaaa ggtgttaggg
     9061 ctagaggata aattcagtgt gcctagcgct agttggattg cggctggaga ttaccatcat
     9121 catttagcag ttaacgaatg gggaggaaaa ggtctggctt cgcgtaagca aggcttacca
     9181 ggtttagcct actatgtcat cgaagtcgca cataaaaaag aactattaac gattgcccaa
     9241 cgagcacaag aagttgacgc accaatcaaa tggatgacat cgagccaatt ggaaatcaca
     9301 gactcagatg gcatcgtgac ccgtattcgt ttagcaaggt aaaattagga atttttaata
     9361 agttataagt attgctagtt tgctttttga acggtctttg gtaacagaac tgcgtgagct
     9421 ggatgggcga gaattttgac aaagaagctg attagagata taatgaagaa aaatcgagca
     9481 aggaaaaaga aatgacaaat tcaaagtata ttacttgttt gaaacgttca gagggccagt
     9541 tgtgtgggat tcaaaagatg attgaaggag atcgtgactg tgctgatatt gtgacgcaac
     9601 tgacagcagt gagatctagt gtagagcgcg tgattgagat gataattacc gaaaatctta
     9661 ctgaatgtat caatcagcca ctagatgatt ctgaagctca aaaggaacgc ctagaaaagg
     9721 ctatccgata cttgattaaa cggaaataaa tgaatgtttt gagacgatat gtgcaaaggt
     9781 ctattaacta ttagaatagt ttaatgtgag tcaaaagaag ttgtaaaaaa tctttaaaat
     9841 taaaaaaacg catagtatca ggtgttgaat gtattgaccc caaaaagtta gattttttct
     9901 gtctaacttt tggggtgcag ttcataagaa ccttggtaat atgcgtttta ttatgtagag
     9961 acttatactc ttcgaaaatc tcttcaaacc acgtcagctt caccttgccg tagatatggt
    10021 tactgacttc gtcagttcta tccacaacct caaaacagtg ttttgagctg acttcgtcag
    10081 ttccatccac aacctcaaaa cagtgttttg agctgacttc gtcagttcca tccacaacct
    10141 caaaacagtg ttttgagctg acttcgtcag ttccatccac aaccttaaaa cagtgttttg
    10201 agctgacttc gtcagttcca tccacaacct taaaacagtg ttttgagcag cctgcggcta
    10261 acttcctagt ttgcacttta attttcattg agtattactt catcttctca tgaaattgag
    10321 tttttgccca gctcctcttg attattctac ttgaccgggc caagcattca taccaccttc
    10381 tacgttggta acggtgaggc cttgggagct gagaaattgg caggtagatg cagagcggac
    10441 tcctccttga caaatgacat agtattcatg gtcaggtttg agttctttgt agccttgctc
    10501 taaggtactt aatggaagat ttttggcacc tggtgcatgt cctgcttgga attcatgtgc
    10561 ttcacggaca tcgataagtt ctagattttc attttggtat tttccataaa agtcagccat
    10621 gctgatacta gtttccatat tctactcctt ctgggttagc cattttgtat agtgaataag
    10681 cgccgtcaag gttttggacg gtaaatcctg cttgcttgag gataggctct gcgatatagc
    10741 tgcgcaaacc actgtggcag ctgacgatgt aggcttgttt tttgtccagt tcatcagctt
    10801 cctccttgac actcggtcag attttgatac aatagtacaa aattagagga ggtgggctat
    10861 gattcagaaa catgcgattc ccattttaga gtttgatgat aatcctcagg cggttatcat
    10921 gcccaatcac gaggggttgg atttgcagtt gccaaagaag tgtgtctatg cttttttagg
    10981 tgaggagatt gaccgctatg cgagggaagt aggggcgaac tgtgttggcg aatttgtttc
    11041 tgccaccaag gcctatccag tttatgtcgt gaactacaag gacgaggaga tttgtctggt
    11101 tcaggctcct gtcggttcag ctccagcagc ccagtttatg gattggttga ttggctatgg
    11161 tgtggagcag attatctcta ctgggacctg tggtgtccta gctgatatag aggaaaatgc
    11221 ctttctagtc cctgttcgcg ctctgcgaga tgaaggagcc agttaccact atgtggcacc
    11281 ttctcgttat atggaaatgc agccagaggc tattgctgcc attgagcgag ttttggatga
    11341 tagagggatt ccttatgaag aagtcatgac ctggtccaca gatggttttt accgagaaac
    11401 ggctgaaaag gtggcttatc gcaaggaaga agactgtgct gttgtggaga tggagtgttc
    11461 cgctcttgcg gcagtagctc aattgcgtgg ggttctctgg ggtgaattgt tgttcacagc
    11521 agattctcta gcggacttgg accagtacga cagtcgtgac tggggctcgg aagcttttaa
    11581 taaggcgcta gaactgagtt tagcaagtgt tcaccacctt tagttgtact ggcaaaggat
    11641 ttgttttatc ataaaatgtc tagctcatac ttttcaaaaa tatgtttaaa cgaggtcacc
    11701 ttcctcttgt cctaggcatg ttgaggttgg gaaaaatctt taaaatcaga aaaacgtatc
    11761 atatcaggtg atgaaaactt tgatactatg cgttttatgt tggtaagatt tagagtgaga
    11821 tgaaatgata ctcttcgaaa atcttttcaa accaggtcag cttcaccttg ccgtatagat
    11881 gttactgact tcgtcagttc tatccacaac ctcaaaacag tgttttgagc aacctgtgac
    11941 tagctttcta agcgatgcct tggttctcat tgcctataat caaaaagaga aattttctcc
    12001 tgaaaagcat atagagtagc tggcgttaaa agctcctgtc ttgctttttt gacctatagt
    12061 cacatctatc aagtattgtt cttgcctaag ctatcaataa aaaggtggca ttttttaggc
    12121 ttggtgttag tgaattttgc cttatcctat ctaagtcatt tcgagctttt tatggtacaa
    12181 tggaaacatg ttattcaaat tatctaagga aaaaatagag ctaggcttat ctcgtttatc
    12241 gccagcccgt cgtatttttt tgagttttgc cttggtcatt ttactaggct ctcttctttt
    12301 gagcttgccc tttgtccaag ttgaaagctc acgagcgact tattttgatc atcttttcac
    12361 tgctgtctct gcagtctgtg tgacaggtct ctcaaccctt ccagtagctc acacctataa
    12421 tatatggggc caaataatct gtttgctctt gattcagatc ggtggtctag ggctcataac
    12481 ctttattggg gttttctata tccagagcaa gcaaaagctt agtcttcgta gccgtgcaac
    12541 tattcaggat agttttagtt atggagaaac tcgatctttg agaaagtttg tctattctat
    12601 ttttctcacg acctttttgg ttgagagctt gggagctatt ttgcttagtt ttcgccttat
    12661 tcctcaactt ggctggggac gtggtctttt tagttccatt tttctagcga tctcagcctt
    12721 ctgtaatgcc ggttttgata atttagggag caccagttta tttgcttttc agaccgattt
    12781 actggtcaat ctggtgattg caggcttgat tattacaggc ggccttggtt ttatggtctg
    12841 gtttgatttg gctggtcatg taggaagaaa gaaaaaagga cgtctgcact ttcatacgaa
    12901 gcttgtacta ttattgactc taggtttgtt gttatttgga acggcaacta ctctctttct
    12961 tgagtggaac aatgctggaa cgattggcaa tctccctgtt gccgataagg ttttagttag
    13021 cttttttcaa acagtgacga tgcgaacagc tggcttttct acgatagatt atactcaggc
    13081 tcatcctgtg actcttttga tttatatctt acagatgttt ctaggtgggg cacctggagg
    13141 aacagctggg ggactcaaga ttacgacatt ttttgtcctc ttggtctttg cacgaagtga
    13201 gcttctaggc ttgcctcatg ccaatgttgc gagacgaacg attgcgccgc gaacggttca
    13261 aaaatccttt agtgtcttta ttatcttttt gatgagcttc ttgataggat tgattctgct
    13321 agggataaca gccaaaggca atcctccctt tatccacctc atatttgaaa ccatttcagc
    13381 tcttagtaca gttggtgtaa cggcaaatct gactcctgac cttgggaaat tggctctcag
    13441 tgttatcatg ccacttatgt ttatgggacg aattggtccc ttgaccttgt ttgttagctt
    13501 ggcagattac catccagaaa agaaagatat gattcactat atgaaagcag atattagtat
    13561 tggttaagaa aggaaagagc atgtcagatc gtacgattgg aattttgggc ttgggaattt
    13621 ttgggagcag tgtcctagct gccctagcca agcaggatat gaatattatc gctattgatg
    13681 accacgcaga gcgcatcaat cagtttgagc cagttttggc gcgtggagtg attggtgaca
    13741 tcacagatga agaattattg agatcagcag ggattgatac ctgcgatacc gttgtagtcg
    13801 cgacaggtga aaatctggag tcgagtgtgc ttgcggttat gcactgtaag agtttggggg
    13861 taccgactgt tattgctaag gtcaaaagtc agaccgctaa gaaagtgcta gaaaagattg
    13921 gagctgactc ggttatctcg ccagagtatg aaatggggca gtctctagca cagaccattc
    13981 ttttccataa tagtgttgat gtctttcagt tggataaaaa tgtgtctatc gtggagatga
    14041 aaattcctca gtcttgggca ggtcaaagtc tgagtaaatt agacctccgt ggcaaataca
    14101 atctgaatat tttgggtttc cgagagcagg aaaattcccc attggatgtt gaatttggac
    14161 cagatgacct cttgaaagca gatacctata ttttggcagt catcaacaac cagtatttgg
    14221 ataccctagt agcattgaat tcgtaaagag ggatgacccc tcttttttga tgcccaagat
    14281 ggcaaataga gacagaagcc ccttgtcttc tagtaaaagt tcttcaaagg ctggacttta
    14341 tggtaaaata gaaggaagtg acaagagaga gtaatactca atgaaaatca aagatcaaac
    14401 taggaaacta gctgcgggct gctcaaaaca ctgttttgag gttgtggata gaactgacga
    14461 agtcagctca aaacactgtt ttga
//
LOCUS       DAVKJD010000043        14182 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKJD010000043 DAVKJD010000000
VERSION     DAVKJD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.043"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..871)
                     /locus_tag="VW157_001333"
     CDS             complement(<1..871)
                     /locus_tag="VW157_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9552625.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYDKEEEYDKKEACLEEI
                     EDLFYEQLPNEEKVWFEATRATIDVIRSGQPEYGETVLDDYFKTIYDKELFLINELEV
                     INLYFAIVLTKIKQGQNQIEEINRIHSFLVRLTNHVELIAPEYLFALSNTLFSGLACL
                     DNLSSYDSLGAYIFSLNHIMEKTQDFQKKPIILMLEWKLSLIINNDYVSAEQFYQKSK
                     LFADIIENSYLVTM"
     gene            complement(943..1503)
                     /gene="rnmV"
                     /locus_tag="VW157_001334"
     CDS             complement(943..1503)
                     /gene="rnmV"
                     /locus_tag="VW157_001334"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9552626.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(1503..2276)
                     /locus_tag="VW157_001335"
     CDS             complement(1503..2276)
                     /locus_tag="VW157_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9552627.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(2581..3234)
                     /locus_tag="VW157_001336"
     CDS             complement(2581..3234)
                     /locus_tag="VW157_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9552628.1"
                     /translation="MTILGKDTVQQSAKGESVTQEATPEYKPETTPGGDKGGNTGNSD
                     ANANAGGGSQAGGPAHTGSQNSAQSQASKQLATEKESAKNAIEKAAKDKQDEIKGAPL
                     SDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIAMVTVPKRPVAPKTT
                     SAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLVVATSGFALLGRKTRRRK"
     gene            complement(3497..3631)
                     /gene="rpmH"
                     /locus_tag="VW157_001337"
     CDS             complement(3497..3631)
                     /gene="rpmH"
                     /locus_tag="VW157_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9552629.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(3776..4990)
                     /locus_tag="VW157_001338"
     CDS             complement(3776..4990)
                     /locus_tag="VW157_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9552630.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(5146..5439)
                     /locus_tag="VW157_001339"
     CDS             complement(5146..5439)
                     /locus_tag="VW157_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552631.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIWQLESLVPLLYPGFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            5601..6053
                     /locus_tag="VW157_001340"
     CDS             5601..6053
                     /locus_tag="VW157_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9552632.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(6088..7476)
                     /locus_tag="VW157_001341"
     CDS             complement(6088..7476)
                     /locus_tag="VW157_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9552633.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            7542..8504
                     /locus_tag="VW157_001342"
     CDS             7542..8504
                     /locus_tag="VW157_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9552634.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQDLKDYMEG"
     gene            complement(8501..8737)
                     /locus_tag="VW157_001343"
     CDS             complement(8501..8737)
                     /locus_tag="VW157_001343"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552635.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVGRTDEVSNHTYGRATLTWFAEIFEEY"
     gene            complement(8801..9811)
                     /gene="ccpA"
                     /locus_tag="VW157_001344"
     CDS             complement(8801..9811)
                     /gene="ccpA"
                     /locus_tag="VW157_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9552636.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(10236..10835)
                     /locus_tag="VW157_001345"
     CDS             complement(10236..10835)
                     /locus_tag="VW157_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9552637.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQSDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERSIADLMQTLYTVLEGRKEYSPELMEVMMTHPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(10836..11933)
                     /locus_tag="VW157_001346"
     CDS             complement(10836..11933)
                     /locus_tag="VW157_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9552638.1"
                     /translation="MLERLKSIDYMYWASLIFMLFPILPVVIGELAVWHLLIDILFVV
                     AYLGVLTTKNQRLSWLFWGIMLVYVAGNTAFVAVNYIWFFFFLSNLLIYHFGVRSFNS
                     LHVRTFLLAQVFVVGQLLIIQRIEVGFLFYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     AKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKCRTLTSELETVKKMLAIAGIEVEIANQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKVYLKLERTEKELILTVSDDGCGFTSIKGNELHTVR
                     DRVLPFSGEVKVISQKQPTEVQVRLAYKERM"
     gene            complement(11933..12670)
                     /locus_tag="VW157_001347"
     CDS             complement(11933..12670)
                     /locus_tag="VW157_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552639.1"
                     /translation="MKRWIALNKIEFLLTKRQLVYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKINNWQKTLRHSPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHFVRGVDMSLGSWIGAALLLIVGSVAFVALGLTLTLLPSS
                     QLMSVVGNLLYFGLAVLGGLWMPVSLFPDWMQAIGKRLPSYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(12672..13556)
                     /locus_tag="VW157_001348"
     CDS             complement(12672..13556)
                     /locus_tag="VW157_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552640.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCSIQEIEV
                     NNRSLLNTIFEETQKGDD"
     gene            complement(13543..13737)
                     /locus_tag="VW157_001349"
     CDS             complement(13543..13737)
                     /locus_tag="VW157_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552641.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFICGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(13734..13940)
                     /locus_tag="VW157_001350"
     CDS             complement(13734..13940)
                     /locus_tag="VW157_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552642.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         4177 a         3289 c         2535 g         4181 t
ORIGIN      
        1 acatagtaac taaataagaa ttttctatta tatcagcaaa tagcttcgat ttctgataga
       61 actgttcggc agaaacataa tcattgttta ttattagaga taatttccac tccaacatta
      121 atataattgg tttcttctgg aaatcttgtg ttttttccat tatatgatta aggctaaaga
      181 tataagctcc tagtgaatca taagacgaca aattatccaa acaggctaga cccgaaaaca
      241 aagtgttact taaagcaaac agatattctg gtgcaattaa ttctacatga tttgtcaaac
      301 gaactaaaaa cgaatgaatt ctgttaattt cttcaatctg attttgacct tgttttatct
      361 ttgtaagcac tatagcaaaa tataagttga taacttctaa ttcatttatc aaaaacaatt
      421 ccttatcata aattgtttta aaataatcgt ccagtacggt ttccccgtat tctggttgtc
      481 cgcttcgaat aacatctatc gttgctctag tagcttcaaa ccatactttc tcctcattag
      541 gaagttgctc ataaaataaa tcctctatct cttccaaaca cgcttctttc ttatcatact
      601 cctcttcttt atcatagatt ggttcacgta aaatctggta tttcaattct aaataagcag
      661 aaggaagagc tgaaaaatcc ggcataaggc tataaactgg aactcctagc cggcgagcaa
      721 tatagtctaa cttgggcaaa ctcggttgcg aagctccact ttcaattcgc gacagttgac
      781 gaactgtcag ttcttgctca tctccacaaa aatctggacg actaattcct ttttctatcc
      841 gaatctcttt aattttttga ccaattaaca tcagacgcct cctcttctac ctgcttcgaa
      901 aaatactgtg aaacttaaaa aattgtaacg gctacatctt tcctactcat aagatttcat
      961 agcttcttcc acttctgcca aagtaacccc aaacaactct aggcgtttga ggagttgctt
     1021 gccgttagaa tagccgattc ggagagtctc tccgagatat tctctacgct tacggctgtc
     1081 tgcccctgct agaaaaccaa ggcgaatcaa atcgctacga ctaatgtcaa actgactctc
     1141 atgttcaaat tgttctgtca cctgagctag agccgttttc aggtcttcat agctggcatg
     1201 ctcaattccc agagaacgcc ccttggtctt ggacttggga acagcttcat ctcgcttgag
     1261 aaaggcatgc tgaactgttg gaatggccgt cataatcatg cgccgaatcc gctccccatt
     1321 aaaatctggg tctgtaaaga caatgactcc atgacgttgg tgtagacgct gaattcgctc
     1381 tatatcctga tcattgatgg cagaacctcg tgtctcatag gtctccacat cgaaataacg
     1441 cttgagattg accgtatcat cgcgaccttc aaccacgata acttgagaaa ttttctcttt
     1501 cattacttgc tgtccaatcc aaaaattcgt tctgcatttg cagtcgttgc taccgccagc
     1561 tcttctgtcg tcataccacg caagtcagcg ataaagtcga ccacatagcg agtataggct
     1621 gttttatttt cacgaccacg cttgggtaca ggtgctaagt aaggcgcatc tgtttccacc
     1681 aacatcttgt ccaaaggtaa ctctttagct gcttcttgga ggtcagttgc cttcttgaaa
     1741 gtcaccactc ctgagaagga aatggtcata ccaagatcca caaacttctc tgcccactca
     1801 agcgtccctg aaaatgaatg catgatacca ccacgaggac caacgccctc actcttgata
     1861 atctcatagg tatcttccag cgcatcacgg gtatggacaa caaaaggcaa atccaagtcc
     1921 ttagatagct gaatctgacg gcgaaaaacc tgctcctgca cctctttagg cgctgtcatc
     1981 caatggtagt ctaagccaat ttcacctaaa gccacaacct tgggatgttt taacttatcc
     2041 aacaagtaag cctcaacttc ctctgtataa gtaccagctt ctgtaggatg ccaaccaata
     2101 gtcgcataga gctgctcata ctcatctacc aactccaagg cacgctcaat cgtcggttta
     2161 tcaaaaccaa caatattcat ctgtgtcaca cccatctcag cagccaaggc aatttcttct
     2221 gcctcacgac ctgcaaattc ttctacattc aagtgtgtat gtgtatcaaa aatcatctct
     2281 tctaacctcg ttttctatct tctattatac caaaaaagag gaggggcacc taatttttcg
     2341 gtttcccctc ctctcttcaa tagagagcta ttctgctatc ttttctatcc gatattgccc
     2401 atctcctatt ccacagttag agacagaaga gattggctac attgatagaa tattcctatt
     2461 cagctaaagt atgtgattat cttgggttct cacttttctc acccatcact aggattgatg
     2521 acttctcaaa atccccctta taatctaact ttaagtagag aatagaattt tctagctgtt
     2581 ctattttcta cgtctagtct ttcttcctag caaagcaaaa ccacttgttg ccaccactag
     2641 accaagacta gcaagtgctg cacttgatgc tgaacctgtg ttaggtaatt gtttgccagc
     2701 aggtgactgg taggtaacat cttgcattgt tcctgcagtt gcttgcggtg cacttgttgt
     2761 cttaggagcc actggtctct taggaactgt aaccatggca atggcttgca ctccaatcgt
     2821 ttctgcttcc ttcacatctt ccatagtttt cgcattttca atctctttga gagctgcttg
     2881 tttttctgct tccactcttg ctaaaagttc tgctttttct ttatcagaaa gcggtgcgcc
     2941 tttgatttca tcctgcttgt ccttggctgc tttttcaatg gcatttttag ctgattcttt
     3001 ttcagtagct aattgcttag aagcttgtga ttgagctgag ttttgtgaac ctgtgtgagc
     3061 tggtccacct gcctggctac caccgcctgc attcgcatta gcatctgagt ttccagtatt
     3121 gcctccctta tctccacctg gtgtagtttc tggcttatac tctggtgtag cttcttgagt
     3181 tacagattca cctttcgcag attgttgaac tgtatctttt cctagaatcg tcactactga
     3241 accatctggg aatgtgattg ttgctgtgcc atctccagct acttcaactt tatagtaaaa
     3301 taaaataata acaggacaaa tcgatcagga cagtcaaatc gatttctaac aatgttttag
     3361 aaacagaggt gtactattct agtttcaatc tactataaca tcgcttccga atccatttgc
     3421 atctactgaa cttttttaca aaaataaaaa tcagtagact cgagttccta ctgatttttg
     3481 atagaattcg tttggattat gcagccaaaa ctttgcgtcc tttacgacga cgagctgcca
     3541 atacgcgacg accattttta gttgacatac ggttacggaa tccgtgtttg cgcgcacgac
     3601 gaagtttact tggttgataa gtacgtttca cgatgaatac ctcctcatag attttgtatt
     3661 cgtttagccg gctataaagt gatcagttac taaacatact ttactattct atctgattct
     3721 ttcacttttg tcaatagctc taggcaaatg tttccagctt tttcgaatgc aagccctatc
     3781 tacgatactg tttcaagaaa cgagtcatct tttcttggat ttgggctaac tcatcaacac
     3841 gaggaaggta aacgatacgg aagtggtctg gttcctgcca gttaaagcct cgaccatgaa
     3901 ccaagagaac cttttcctgc ttcaagaaat caaggacaaa ctgctcatca tcatcgatac
     3961 ggtacatatt gcggtcgatt tttgggaaga tatagagccc cgccttgggt ttaacggcag
     4021 acaaacctgg aatatcttga atggcattat agatgaaatt tctttgctcg tagattcgtc
     4081 caccaggaag aagcaattca tcgactgatt ggtgaccccc caaggaagtt tgtacgactt
     4141 gttgggccaa aacgttagag caaaggcgca tattggacag catattgagc ccttcgatat
     4201 agcccttaac atgagtctta gggccagaca agaccatcca acccacacgg aaacctgcta
     4261 tgcggtggga ttttgacaga ccattcatgc tgacacagaa gacatctggt gccaagctcg
     4321 ccacaggcgt atgcacatgt ccgtccatta ccatgcggtc atagatttca tccgcaaaga
     4381 taatcaaatc gttttgacgg gcaatctcaa taatctccag caagagttcc ttaggataaa
     4441 gggctccagt tgggttattt ggattgataa ggacaattgc cttggtattg gaagtgattt
     4501 ttgacttgat atcgtcaata tctgggtacc attccacagc ttcatcacag atatagtgaa
     4561 cggcatttcc tccagctagg ctgacagccg ccgtccatag aggatagtct ggcattggca
     4621 ctaagacctc atccccattg tccaaaagcc cctgcatgga catgacaatc aactcactga
     4681 caccatttcc aaggtaaata tcatcaatat ctacgtttgg gaatttcttc agctggcaat
     4741 actgcatgat ggccttacgg gctgagaaaa tccctttgga gtcagaatag ccttcactat
     4801 cacgtgcatt cataatcaag tcatgaatga cctcgtctgg cgctgtaaag ccaaattctg
     4861 ctggatttcc tgtattcaga cgtaaaatct tttctccatt cgctcgcatc cgcatggctt
     4921 cttccaatac aggaccacgg atatcataag caacatgctc taacttacta gacttgttat
     4981 attctttcat cttgtttcct caattcatca ggatagtaac attataccac tagaaagaga
     5041 atgcgacaag tttactgaaa tgtgttacta gtttcgtata gaaaaaaaac cttgctaaag
     5101 gcgaggttaa gaacgtagag atagcaggta ttttgcaaag acacatcaca taccaaaaga
     5161 taaacctaga aaaagcaagc ctagaaagga gtcataaaga agattaaaca tgaggaaaag
     5221 cccccatatc atcaagtaat cccaacgccc acttcgtttt gcaactagaa ataagattag
     5281 atggtagagt acatgaaaga ctaaaaatcc aataaaacct ggataaagaa gcggtaccag
     5341 actctctaat tgccagatca tgatcacttc taccaagact gaaactagga taattccata
     5401 atacaggaaa tttgattttt gaaaagagtt tttcatcatt ctcccttcct tcacttcttt
     5461 cctgctatcc tttcttttat tttatcatat atttatatag attttaagcc ctaatattct
     5521 tttgaaaaat actttttttg ctttacttct atagtgaaaa ggtttacaat tatagtaaga
     5581 aaatttcagg aggtttcatt atggcacaac gttaccaaaa tatcatggtc gcaatcgatg
     5641 gttctaagga agcggacttg gcttttgtca agggagttca ttctgctcta cgaaacgacg
     5701 ctaaactcac catcgcacat gttattgaca cacgcgctct ccaaagcgta tccacctttg
     5761 atgctgaagt ttacgaagaa ctccaagtcg acgctgaaag tctgatgaaa gagtacgaaa
     5821 aacgtgctaa agatgctggg gtagcagatg ttcatatcgt cattgaaatg ggaaatccaa
     5881 agaccctgct agcacgtact attccagatg ccgaggaagt ggacctcatc ctcgttggcg
     5941 caactggtct caacgccttt gaacgcctct tggtcggctc ttcatctgaa tacatactcc
     6001 gccatgctaa ggtcgattta ctggttgtga gagaacaaga aaaaacctta taatcacaaa
     6061 gaaaaggagc ccctagctcc tttttgttta cgatttattt ctctctttat ggcgttcgta
     6121 agccttgagc tggcgctgca gttccttttt aatagcaggt tctggagcat atttttcttc
     6181 ccaattatct ggttttaaga ttttatgggt cactggatca aaatgagcct tgccatctgg
     6241 aaaaattttc cccatattgg cctgatggac aatatcaaaa atacgttctg ggtccacccc
     6301 catcaagaca aaactgccgt aggtgaagta aagcgtgtca atcaaggcat ccacttgccc
     6361 tatcaaatct tgctgagcag gtgtcttctt ggctacttta tctgctgcct tatcaagggc
     6421 ctgatgaagt tgcgatacag cttgaccaaa atcttcttca gaaggactgg ctgctcgaac
     6481 aaactccacc aattcttcta ttttaaaacc agccctatgg gttgcaccct ctaaatccca
     6541 agctcgaggt tcttcttggg ttcgttcatc catcatgtgg tggaaggtct tgaccttatt
     6601 gaaatgatag tcacggctga caaagacttt ttctgaagac aaaacaccaa aatgttccaa
     6661 ggccttgtgg attccatctt gttgattgct agtggtaatg tgcttggcag cttccttgac
     6721 actgctacta ccatttccca tagcgaccga cataccgacg ccagccagca tttcaaggtc
     6781 attatctgag tcaccaaaag ccatgacttg gttgaggtca aagccatatt ctttcccaac
     6841 tcggcgaatg ccttctaatt tagaatttcc ctgattgatg acatccgatg caaaaggatt
     6901 gctacgtgtc aatttcaagt cttcaaaatc agctgccgcc ttctcagatt cttctggtgt
     6961 catcaacatc aaaacttggt agataggctg attcatcagg tgaagcaggt cctcttcctt
     7021 ttggggaaca accttgctga ccatgcgatt aaaagactga ctcaccgtcc gagttaaaac
     7081 agagggaacg aagcgactaa ttcgttggga aaaagaaccc agaccaaagg acatgatttt
     7141 agaacccaac atggcatcct tggtccctag agcaatctcc gtgccctctt ttttagcata
     7201 gctaattaga tggcgcaaat gtaacttgga aatagggctc gtgaacaaga ctctgtcttt
     7261 actaaagata tactggccat tataggttac cgcaaaatcc agatccaaat cgtccatcaa
     7321 ttccttaaca aaaaaaggtc ctcgccctgt cgctacgcca actagtaccc cttgttcttt
     7381 gacaatctta atcgcatcct tagtggattt caaaacactc ttgcgattgt tgaccaaggt
     7441 tccatcgata tcaaaaaaaa cagctttgac ttccatccta tcccaatctc cccttttgtg
     7501 atacaatgat tataccacat ttcagaaaga gttagtaaat catgcctaag aaaatccttg
     7561 ttttacatac gggtggaact atttccatgc aggccgatgc ttctggcgct gttgtgacga
     7621 gttcagataa tcccatgaac catgtgtcca acccacttga aggaatccaa gtccacgcct
     7681 tggacttttt taaccttcca agtccccata tcaaacccaa acatatgctg gtcctctacc
     7741 agaaaattaa agaggaagca gataactacg atggagtggt gatcacacac ggaaccgata
     7801 ctttagagga aacagcctat ttccttgata ccatggaagt tccccatatg cctatcgttc
     7861 taacaggagc catgcgtagc tccaatgaac tcggtagtga tggtgtttat aattacctaa
     7921 gtgctttacg agtggccagc gatgacaggg ctgctgacaa aggagttttg gtcgttatga
     7981 acgatgaaat ccacgctgcc aagtatgtca ccaaaacaca tacgactaat gtcagcacct
     8041 tccagactcc aacacatggc cccctcggtc tcatcatgaa acaggaaatc ctctacttca
     8101 aaacagctga acctcgtgtt cgctttgacc ttgatcacat acaaggttta gtccctatca
     8161 tctcggctta tgctggtatg acagatgagc tgattgatat gctggattta gaacacttgg
     8221 acggtttgat tatccaagcc ttcggagctg gtaatattcc caaagaaacg gctcaaaaat
     8281 tagaaagcct tctgcaaaaa ggaatcccag tcgctctggt atcacgatgc tttaacggta
     8341 ttgccgagcc tgtttatgcc taccagggtg ggggcgtaca gttgcaaaaa gcaggcgttt
     8401 tctttgttaa agaactcaac gcccaaaaag ctcgcttgaa actcctcatc gccctcaatg
     8461 ccggactaac aggacaggat ttgaaagact atatggaagg ctaatactct tcgaaaatct
     8521 ctgcaaacca cgtcagagtc gccctaccgt atgtatggtt actgacttcg tcagttctac
     8581 ctacaacctc aaaaacatgt tttgagctga cttcgtcagt tctatctgca acctcaaagc
     8641 agtgctttga gctgacttcg tcagttctat ctacaacctc aaaacagtgt gttgcgcagc
     8701 ctttaaccag ccgcctagtc cgctctatgg tattcattaa gtcaacatct cttgtttaag
     8761 agcacaaaat caggaaatct tctcgattcc ctgatttttt ctatttacgt tttcgtgttg
     8821 agctacgttc tgtcaaacca tgaggtaaga gaacttcacg ttcttccaac tcttccttat
     8881 gcataatctt ggtcaacata cgcatactaa tggcaccaag gtcataaaga ggttgggcaa
     8941 tcgttgtcaa gtttggacgg gtaaagcgtg agatttgtga atcatcacta gtaataattt
     9001 caaaatcttc tggcacagaa acacctttat cagccaaacc gttcaagact cctgctgcca
     9061 actcatcacc tgtcacaact gctgcagttg catttgatga aatcaaacgc tctgctaagg
     9121 cgtaaccatc atcatagcta tatttagatt caaataccaa accctcacta taagtgattc
     9181 ctgctttttt caaggtttcc ttgtagccaa ctaaacgaac cttaccattg atgtcatcca
     9241 ctagcggacc gctaacgaaa gcaatacgct cattttcttt agcaaggtaa ctcactgcat
     9301 caattgttgc ttgcttatag tcaatattga cacttggcaa ctggtgctca acatcgacag
     9361 ttcctgcgag aacaatcgga gtacgtgaac gcgaaaattc tgagcgaatt ttatctgtca
     9421 agtgataacc catatagata atgccatcta cctgctttga aaagagggta ttgacaacag
     9481 aaacttcttt ctcgttatct tcatcgctat tagctaggac aatattgtac ttgtacattt
     9541 ctgcaatatc atcaatcccc ttagccaaac tcgaaaaata accattggta atatttggaa
     9601 tcacgacacc gacagtggtt gtctttttac ttgcaagacc acgcgcaact gcatttggac
     9661 gataatccaa acgatcaatt acctctagca cttttttacg ggtattctct tttacatttt
     9721 tattgccatt gaccacacgg ctgaccgtcg ccatggaaac acctgcttca cgagcgacat
     9781 cataaatggt tactgtatca tctgcattca ttccttttcc tgtcctttct atctcacaca
     9841 ttcttttaca agtagaggtg ctgattgaag ctctatatct acttacaaaa gtgaagatgt
     9901 gaaaatttcg ttttcatatt tctacttatt ccattctatc actaattgta aacactttca
     9961 agtgtttttt gaagattgat tgaaaaaatt tcatagaaaa cctaggttta gctccttgct
    10021 accaccttag actaaacaaa aaggaggaaa ctaagccctc ctaaagttat agtaaaatga
    10081 aataagaaca ggataaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaag
    10141 tagaggtgta ctattctagt ttcaatctac tataggtatt gttccattca ctaccgtcaa
    10201 ttttagcaca tagtcttcat gaaaatatta tatcatcata accaaccaga ttcttttgcg
    10261 atattagctg cctctgttcg attaccagca tccagttttg aaagaatatt ggtgacatag
    10321 tttcgaatag ttccgtttga gaggtaaagc tgatctgcga tttcttggtt ggataagccc
    10381 cgggcgattc cctttaagac agcaatctct tgttctgtca acggattggg atgcgtcatc
    10441 atcacttcca tcaattcagg agaatactcc ttgcgtcctt cgaggacggt gtacaaggtt
    10501 tgcatgagat ctgcaatgct tcgttccttt aatacataag catccactcc agccttgacc
    10561 gcacgttcaa aataccctgg gcgcttgaag gtcgtcacta caaccacctt tgtttcaagc
    10621 ttttctgctc gtatccactc caagacttca agacctgtct taacaggcat ttctacgtcg
    10681 aggatggcga tatctacaga ctccttttct aatagctgga ttgcttcttg cccattcttg
    10741 gcttgaagaa cagactccac gtccgattga agcgtgagca attgacacat agcatctcgc
    10801 aacatacttt gatcttctgc tactaatact ttcatctaca ttctctcctt ataagctagt
    10861 cgaacctgaa cttctgtcgg ttgtttttga ctaatcacct ttacctctcc cgaaaatgga
    10921 aggacacgat ctcgaactgt atggagctca ttccctttta tagaagtaaa accacagcca
    10981 tcatcactta ctgttagaat gagttccttc tctgtccgtt ctaattttaa gtagacttta
    11041 gacgctttag catgtttgat gatgttggtc actaattcaa gcaaaatcat ggaagccgtt
    11101 gactccaatt cctgagttaa gctagctgta tctagttgat tagctatttc cacctcaatt
    11161 ccagcaattg ctaacatctt tttcacagtc tctagttcgg atgtcaaagt tctacactta
    11221 agattttcca caatggttcg cacttcattc atcgaatcct tacttatctg gtgaatttct
    11281 tttaattcct tttccacctg tggataagcc tccatctgaa ataactgcaa ggctaaatct
    11341 gtcttgacac tcagcatggc aaaggtatgt cccaagctat catgcaagtc ctgaccgata
    11401 cgactacgtt cattttcagc tagcaataga tttatctggg cattttgctt ggcctgcgct
    11461 tctttcaaat cctctacaat acgaatccga accaaaccaa aagtcatcaa atcgacaaaa
    11521 gcaagaatta caagtagata gaatagaaac ccaacttcga ttctctgaat aatcaacagt
    11581 tgccccacca caaacacttg agcaaggaga aaagtccgga catgtaaaga attaaaacta
    11641 cgtacgccaa aatgataaat taagagattg gaaaggaaaa agaagaacca gatataattt
    11701 acagcaacaa aggcggtgtt cccagctaca tagaccagca tgatgcccca gaaaagccaa
    11761 gataggcgct gattcttagt tgttaaaaca cccaaatacg ccactacaaa tagaatatca
    11821 atcaataaat gccagacagc aagctcccca atcactacag gaaggatggg aaaaagcata
    11881 aaaatcaaac tggcccaata catatagtct atgctcttca gtctttcaag cattaagcat
    11941 tctccttatg accttgaagg taaatggtca aaccaaacaa aactgctgaa aaaacaagta
    12001 gataaactgt ggctgataaa ttgatgccac cctcatttaa gaaggtcttg agcaactcca
    12061 tcaactgata gcttggtagg cgcttcccga ttgcttgcat ccagtctgga aataaagaga
    12121 caggcatcca gagtccgcct aaaacagcca agccaaagta aagaagattg cccacgacag
    12181 acatcagctg actggacggt aagagagtca gggttaagcc aagtgctaca aaggcaacac
    12241 ttcctactat cagcaaaagc gcagccccaa tccagcttcc aagagacatg tccacacctc
    12301 ttacaaaatg cccaactgag aaaacaacca gaattgagac caaataatca accatcatac
    12361 ttgttatctt tgatagataa tattctacca tatttacagg actatggcgc aatgttttct
    12421 gccagttgtt gatcttgtcg gtatgtaaga cggctgggaa tgaaaagata gctgtcgaca
    12481 tcatggaaaa agccgtcatg gagatgaggt aatcacgcat aaaattcgca ggtccaccgg
    12541 gtgtgtcctg gtacatgcct gaaaagaata aataaaaggc cgtcggcatc cctactgata
    12601 ataaataata gactagttgc cgtttggtta ataggaattc tatcttattt aatgcaatcc
    12661 atcgtttcat cttagtcatc tcccttttgt gtttcttcaa agattgtatt caacaaacta
    12721 cgattattaa cttcgatttc ttgtatgcta catcctgctt gggctaatag ttcccagaaa
    12781 gcatctgctt ctcgagtgac tacttgtaaa gaatcttgct ttagggtcca gttttcaacc
    12841 aaatttgact gctctacgac ttccttgtaa gctataggaa ggataaagtg cttttctatt
    12901 tcctcgctac gcatagctag aggcgtcgta tctcgaatca actctccctt atttaagagc
    12961 aaaatgcggt ctgcagtgtg ttccacttcc tcaatataat gggacgaata gagaatggtg
    13021 actccttgcg cttttaactc ctggacaatt tcccaaaaac gttgacgagt tgaggtatcc
    13081 atggacgcag taggctcatc taaaaaaaca atctttggtc gcccaattaa ggtcaagacg
    13141 aaagaaaaga gacgcttttg accgcctgat aatttttctg ccaactgttc tttttgttgc
    13201 ttgacaaact gcaatagttg atcaatttcc tgattgctca aaggatttgg atagatacgt
    13261 tgaaagaaag caatcaactc tttaactttt aatttctgaa cgattacatt ttcttgaggg
    13321 aggtaagccc tagtatagtc taacttagaa ctcgtcactg gtaaaccttg aatagatact
    13381 tgaccgcttg tgaccagttt atctccaagc aaacaatcta agagtgttgt ctttccggca
    13441 ccatttggac caattaaggc aacacattca ccatcgttga tttcaaagga gatgttccgc
    13501 aaaatttcct tgtcttttat tttcttactc aatttctcaa ctttaaccac agtcatgaga
    13561 ctctcctttc aaccactcca ttcccatagg gaaaacgacg aaaatcataa atccaaaccc
    13621 caaagcacca caaatgaatt ggcgaagcaa ggcttgatca aaccaatcag taaacatttc
    13681 cactaaccat accaagagtg acaggccgat aaagaaatag atgatccctc tcttcattcc
    13741 tcaaactcct ttttcacatc tctgactaat ttcaaacctt ctctaacaag ccaagacatg
    13801 attccaaagc cagcaaagac cttccaagga aaatggtaga aatcctcatc caatcccgaa
    13861 aacatgagat aggtcataac tcctgctact actaaactta ctgcgataat cattttattt
    13921 ctcatctctt cttcccccat ttcatactac aattatagtc ttttaaaatc agaggagaca
    13981 gaagcttctg tcactagaaa atatgacaaa tgtcataaaa aattctgttc aaaacaagca
    14041 agatacacta tacaataaaa cacaatcaga aaaatctaag gcaacttcct caaaagagat
    14101 atcaaaccca attcacacca taatgtaaac taatacttat ttaaaatcaa aaagagtaga
    14161 aatttttatc agacaaacac at
//
LOCUS       DAVKJD010000044        14096 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKJD010000044 DAVKJD010000000
VERSION     DAVKJD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14096
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.044"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            354..1517
                     /locus_tag="VW157_001351"
     CDS             354..1517
                     /locus_tag="VW157_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9552643.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1514..2191
                     /locus_tag="VW157_001352"
     CDS             1514..2191
                     /locus_tag="VW157_001352"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9552644.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2181..3032
                     /locus_tag="VW157_001353"
     CDS             2181..3032
                     /locus_tag="VW157_001353"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9552645.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKY"
     gene            3054..4121
                     /locus_tag="VW157_001354"
     CDS             3054..4121
                     /locus_tag="VW157_001354"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9552646.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTELNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4131..5297
                     /gene="aroC"
                     /locus_tag="VW157_001355"
     CDS             4131..5297
                     /gene="aroC"
                     /locus_tag="VW157_001355"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9552647.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRCTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5307..6410
                     /locus_tag="VW157_001356"
     CDS             5307..6410
                     /locus_tag="VW157_001356"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9552648.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6421..6759
                     /locus_tag="VW157_001357"
     CDS             6421..6759
                     /locus_tag="VW157_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9552649.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6851..8134
                     /gene="aroA"
                     /locus_tag="VW157_001358"
     CDS             6851..8134
                     /gene="aroA"
                     /locus_tag="VW157_001358"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9552650.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKIIVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8127..8603
                     /locus_tag="VW157_001359"
     CDS             8127..8603
                     /locus_tag="VW157_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9552651.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDTNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8600..9448
                     /gene="pheA"
                     /locus_tag="VW157_001360"
     CDS             8600..9448
                     /gene="pheA"
                     /locus_tag="VW157_001360"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9552652.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFDNITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWLLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9445..10719
                     /gene="psr"
                     /locus_tag="VW157_001361"
     CDS             9445..10719
                     /gene="psr"
                     /locus_tag="VW157_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9552653.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFVYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10998..11843
                     /locus_tag="VW157_001362"
     CDS             10998..11843
                     /locus_tag="VW157_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9552654.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKKT
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11833..12936
                     /locus_tag="VW157_001363"
     CDS             11833..12936
                     /locus_tag="VW157_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9552655.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12938..13924
                     /locus_tag="VW157_001364"
     CDS             12938..13924
                     /locus_tag="VW157_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9552656.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13940..>14096
                     /locus_tag="VW157_001365"
     CDS             13940..>14096
                     /locus_tag="VW157_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HEU9552657.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWN"
BASE COUNT         4156 a         2600 c         3293 g         4047 t
ORIGIN      
        1 aaaaggcttc tctataaaat caaattttcc agatacctat tgaagcagat ttatttcttc
       61 atctttcttc tactaccttt gtcactaaat ttgacagaca catattgctg attcaacctc
      121 tctacctata atctcacagt taacctgagg ctagtttcat agtttgctat ttgattttcg
      181 ttgataaaaa gaaatgctcc tccaagacgg aagagcattt tttattgtga ttcttacaca
      241 tttacttcct agttttgcag gaagtctagt ataccatttt ttaaaagaag agtaaaaaag
      301 caagggtagt ttcttttttg agaattgtct aaaagtttga tataatggta gttatgaata
      361 gaataagagt cagcaaaagg gttgaaaaga agcttgctaa ggggctagtt ttactagaag
      421 ccagtgatct tgagaatgtc aatcttaagg atcaggaagt agaggtgcag ggtcaggaag
      481 gaaactttct tgggactgcc tacctttctc agcaaaacaa gggcttgggc tggtttgtca
      541 gcaaagacaa ggtggccttc aatcaagctt tctttgaaac gttgtttaga aaagccaaag
      601 aaaagagaaa cgcctactat caagatgatt tgacaactgc ctttcgtctc tttaatcaag
      661 agggagatgg ctttgggggt ctgacagtgg acctttatgg cgactacgcc gtcttttctt
      721 ggtataactc ttatgtttat cagattcgtc agactatatc agaagccttt agacaggttt
      781 tccctgaggt tttaggggct tatgagaaaa tccgctttaa gggtttggac tatgaatctg
      841 cccatgttta tggtcaagaa gcacctgact ttttcactgt tttagaaaat ggtgtcctgt
      901 atcaagtctt tatgaatgat ggcttgatga caggaatttt cctagaccag catgaggttc
      961 gcggtagttt agttgacggc ttggctatgg gtaaatcctt gctcaatatg ttttcctaca
     1021 cagcggcttt ttcagtagct gcggccatgg gaggagctag ccagacaact tctgttgatc
     1081 tagccaaacg ttcacgagaa ttgtctcaag cgcattttca ggcaaatggg ctcagcacag
     1141 acgagcatcg ttttatagtc atggatgtct ttgagtattt caaatatgcc aaacgcaaag
     1201 acttgaccta cgatgtgatt gtcctagatc cgcctagctt tgctcggaat aaaaaacaaa
     1261 ctttctctgt ggccaaggat tatcacaagt tgatttccca gagtcttgag attttaaatc
     1321 cgggagggat tatcattgcc agtactaatg ctgccaatgt ttcccgtcag aaatttacag
     1381 aacaaattga taaaggcttt gcaggaagaa gttaccagat tttaaacaaa tatggtcttc
     1441 cagcagattt tgcctataat aaaaaagatg aaagtagtaa ttacctcaag gtgattagta
     1501 tgaaggttag taaatgaaat taatcgtttc agtaatgcca agaagtttag aggaggctca
     1561 ggctctggat gccacgaggt acctggatgc cgacatcatt gaatggcgtg ccgactatct
     1621 gcctaaagaa gcgattttgc aggtagctcc agccattttt gaaaaattcg caggccgtga
     1681 gttggttttc acgctacgaa ctcgctccga aggtggagaa atcgaccttt ctccagaaga
     1741 gtatatccat ctaatcaagg aagttgcgca attctatcaa ccagactata ttgattttga
     1801 gtactatagc tacaaggatg tttttgagga aatgctggac ttcccaaatc ttgttttgag
     1861 ttaccataat ttccaagaaa cacctgagaa catgatggaa atcttgtcag agttgacgat
     1921 cctaaatcca aaacttgtta aggttgcggt gatggctcac acggagcagg atgtcttaga
     1981 cttgatgaac tatacacgag gctttaaaac cctcaatcct gaacaggaat atgtgaccat
     2041 ttctatgggt aaggtgggca aggtctctcg tatcactgcg gatgtgactg gttcgagttg
     2101 gtcttttgcc agtctggatg aggttagtgc ccctgggcag atttctctag ctagcatgaa
     2161 aaaaatcagg gagattttgg atgaagcttg atggctatac acgtttagct gcagttgttg
     2221 ccaatcctat taagcattct atttctccct tcatccacaa tagagccttt gaggcgacag
     2281 ctaccaacgg tgcttatgtg gcttgggaga ttgaagcgag tgacttggta gaaacagtgg
     2341 ccaatattcg tcgctaccag atgtttggca tcaatctgtc catgccctat aaggagcagg
     2401 tgattcctta tttggataag ctgagcgatg aagcgcgctt gattggtgcg gttaatacgg
     2461 ttgtcaatga gaatggcaat ttaattggat ataatacaga tggcaaggga ttttttaagt
     2521 gcttgccttc ttttacaatt tcaggtaaaa agatgaccct gctgggtgca ggtggtgcgg
     2581 ctaaatcaat cttggcacag gctattttgg atggcgtcag tcagatttcg gtctttgttc
     2641 gttccgtttc tatggaaaaa acaagacctt acctagacaa gttacaggag cagacaggtt
     2701 ttaaagtgga tttgtgtgct ttagaatatg tttctgaact gcaagcaagg attgccgagt
     2761 cggatttgct agttaatgcc accagtgtgg gcatggatgg ccaatcctct cctgttcctg
     2821 aaaacatagt cttaccagaa actctcttag ttgcagatat catataccaa ccctttgaaa
     2881 caccgttttt gaaatgggct agaagtcagg gcaatccagc agtcaatggt ctgggaatgt
     2941 tactctatca agctgcagaa gcttttcaac tgtggacagg caaggaaatg ccgacagaag
     3001 agatttggca gtctttaaca gaaaaatact aataaagaaa gggagactct cctatgaaaa
     3061 tcagaatcga tattcctcat catccttatg atattcagat tgaaaaaggt tgtatggccc
     3121 aggctggtca gtggttgcga gaactctggc aaccgcaaaa ggtagtcatt gtgacagata
     3181 accatgtagc ctctctctat gcagagaagg tcaagctcag cctagaagat gctggttttc
     3241 aggtagctgt ttttgatttc ttagaaggtg aagaaagaaa gaatttaact actgttcaga
     3301 aagtctatga atttctagtc aagcaaggtc tgactcgtag cgatggaatc gttgctcttg
     3361 gtggtggcgt tgttggggac ctggctggat ttgtagcctc tacctatatg cggggtattc
     3421 actttgttca gattccgact agtttgacag ctcaggttga ttcttctatc ggtggaaaga
     3481 caggcgttaa cacaccattt gctaaaaata tggtggggac ctttgcccaa ccagatgggg
     3541 ttctgattga tccacttgtt cttgaaaccc tcggaaaaag agagttgatt gaagggatgg
     3601 gtgaagttat caagtatggc ttgattgagg atccagaact gtgggctctc ttgacggaac
     3661 tgaatggttc tgttgagagc attttggaac atgcagagac cttgattgaa cattcttgtc
     3721 aggtgaagcg caagatggtg gttgaagatg agttggacaa tggtattcgt ctttacctca
     3781 attttggcca cactattggc catgccatcg aagcgactgc cggttatggc aaggtcatgc
     3841 atggagaggc tgttgccatg ggaatggtac agatttccaa ggttgctgag gaaaaaggcc
     3901 tcatgccagc tggcattacc caatctatca cagagatgtg tcagaaattc ggcttgcctg
     3961 ttgactatga aaattgggaa gttgacaagc tttatcaggc tcttactcat gacaagaaag
     4021 cgcgtggtaa caccttgaaa ttggtcttgg tgccagagct tggttcagcg accattcatc
     4081 cagtttctct ggaagagatg aaagactact tggtaaaata aggagagtgt atgagatatt
     4141 taactgcagg agaatcacac ggcccccgtc taacagctat tattgaggga attccagctg
     4201 gacttccatt gacagctgag gatatcaatg aggaccttag acgccgtcag ggtggctacg
     4261 gtcgtggtgg tcgtatgaag attgagagtg accaggttgt ctttacttcg ggcgttcgcc
     4321 acgggaagac gacaggggcg cctattacta tggatgtcat caataaggac caccagaaat
     4381 ggctggacat catgtctgcg gaggacattg aagaccgcct taaaagcaag cggaaaatta
     4441 ctcatcctcg cccaggtcat gccgatttgg ttggggggat taagtaccgt tttgatgatt
     4501 tgcgaaattc tttggagcgt tcatcagctc gtgaaaccac catgcgggtg gcagttggtg
     4561 cagtagccaa acgtctcttg gctgagctgg atatggagat tgccaaccat gtcgtggtct
     4621 ttggtggcaa ggaaatcgat gttcctgaaa atctgacagt cgctgaaatt aagcaaagag
     4681 ctgcccagtc tgaagtttct attgtcaacc aagaacgaga acaggaaatc aaggactata
     4741 ttgaccaaat caaacgtgat ggtgatacca tcggtggggt tgtggagaca gtcgtcggag
     4801 gcgttccagt tggtcttggt tcctatgtcc aatgggatag aaaattggat gcaagattgg
     4861 ctcaagctgt tgtctctatc aatgccttta aaggggtgga atttggtctt ggctttgagg
     4921 ctggttatcg taaaggcagc caagttatgg atgaaattct ctggtctaaa gaagacggtt
     4981 atactcgctg taccaataat ctaggtggtt ttgaaggtgg tatgactaat gggcaaccca
     5041 tcgttgttcg tggggttatg aaacccattc ctactcttta taaacctctt atgagtgtgg
     5101 atatcgaaac ccacgaacct tacaaggcaa ccgtggagag aagtgatccg actgctcttc
     5161 cagctgcagg aatggtcatg gaagcagttg tagcaacggt tctggcgcaa gaaatcctcg
     5221 aaaaattctc atcagataat cttgaggaac taaaagaagc ggtagccaaa caccgagact
     5281 atacaaagaa ctattaagga gttcctatgg caaaaacaat ctatatcgca ggtcttgggt
     5341 tgattggagc ctctatggca cttggtatca aacgcgatca tccagattat gaaattttag
     5401 gttataatcg tagtcaagct tcgagagata tcgccttgaa agaaggcatg attgaccgtg
     5461 caacggatga ttttgctagt tttgctcctt tggcagatgt cattatcctc agcttgccaa
     5521 tcaaacaaac tattgctttc attaaggagt tggccaattt ggatttgcga gaaggcgtta
     5581 ttatttcaga tgctggttcg accaagtcaa ccattgtgga tgcggcggag cagtatttgg
     5641 ctggcaagtc tgttcgcttt gtcggggccc atcccatggc tggtagtcac aagacagggg
     5701 ctgcttcggc agatgtcaat ctttttgaaa atgcctatta tatctttaca ccttcaagcc
     5761 tgacaagtca ggacacgctt aaggaaatga aggatctact ttcaggtctt catgctcgtt
     5821 ttatcgagat tgatgccaag gagcatgatc gtgtcacttc tcagattagc cattttcctc
     5881 atattttggc ttctagtctc atggagcaga ctgcggtcta tgctcaagag catgagatgg
     5941 caaggcgctt tgcggcaggt ggttttcgag atatgacccg aattgcggaa agcgagccag
     6001 gaatgtggac ctccattctc ttgtccaata gcgagatcat tctggataga attgaggatt
     6061 tcaaggaacg tttggaagcg attggtcagg ccattagtaa gggagatgaa gagcaaattt
     6121 ggaacttttt taaccaagcg cgtgaacaac gccagaccat ggaaatccat aagcgtggtg
     6181 gtgtggatag ctcttacgac ctctatgttg acgttcctga tgaagaagat gtcatcctgc
     6241 ggattttgga actgctacgt ggaacttcct tggttaacat ccacatcaac gaggaaaatc
     6301 gtgaggatat tcacggaatc ctacaaattt catttaaaaa tgctcaagac ttggaaagag
     6361 ctgagcatct cataacagaa aataccgact atacagtcgt catcaaataa ggagaagaac
     6421 atgtcaaata tttacgatag tgcaaacgaa ctcagtcgcg gtctacgcgg attaccagaa
     6481 tacaaggctg ttaaagcagc taaagatgcg attgcagcag atgctgaggc aagcaaaatc
     6541 tttacagaat atcttgcctt tcaagaggaa attcaaaaac tagcacagac aggtcaaatg
     6601 ccagatgcgt ccttccaagc taagatggaa ggctttggta aacagattca aggaaatagc
     6661 ctcttgtcag aattctttac caagcaacaa caattggcca tttacctttc tgacattgaa
     6721 aaaattgttt ttgaaccagt ttcagaattg ctgaaataag aaaagaacga tcctaaagtc
     6781 aggaattttt aaggaatttc tggcttttta tgataaaata ggtaaaagta ttgcaagtat
     6841 gaggtccagt atgaaactaa aaacaaacat tcgccattta catggtagta tccgcgtccc
     6901 aggtgacaag tctatcagcc accgttccat tatctttgga agtttggctg agggtgagac
     6961 caaggtttat gatattctgc gaggcgaaga cgttctttcg accatgcagg tttttcgtga
     7021 ccttggtgtt gaaattgagg ataaagatgg ggttattacc attcaaggtg taggcatggc
     7081 tggcttaaaa gcgccgcaaa atgcccttaa tatgggaaat tctggcacct cgattcgcct
     7141 gatttcaggt gtccttgctg gtgcagattt cgaagtagag atgtttggag atgatagtct
     7201 ttccaaacgt cctatggacc gtgtgaccct tccactgaaa aaaatgggcg tcagcatttc
     7261 agggcaaact gaacgagact tgcctcccct tcgcttaaaa gggacgaaaa acctaagacc
     7321 tattcattat gagttgccaa ttgcctctgc ccaagtcaag tcagccttga tgtttgcagc
     7381 cttacaagct aagggggagt cagttattat cgaaaaagag tgcactcgta atcatactga
     7441 agatatgttg aaacaatttg gtggtcattt aagtgtggac ggtaagaaaa tcatagtcca
     7501 agggccacaa aaattgacag gacagaaggt ggtcgtacca ggagatattt ccagtgcagc
     7561 cttttggtta gtcgcaggtt tgattgttcc aaattctcgt ctagtgctgc agaatgtggg
     7621 gatcaacgaa actcgcaccg gtattattga tgtcattcgt gccatgggtg gaaaattgga
     7681 aataactgaa atcgatccag tcgctaaatc tgcaaccttg attgttgagt cttctgactt
     7741 gaaaggaaca gagatttgtg gcgctttgat tccacgtttg attgatgaat tgcctattat
     7801 tgccctactt gcgacccaag cccaaggtgt aacagttatc aaggatgctg aggagctcaa
     7861 ggtcaaggaa acagaccgta ttcaggttgt ggcagacgcc ttaaatagta tgggagcaga
     7921 tattactcct acggcagatg ggatgattat caaaggaaaa tcagctcttc acggtgctag
     7981 agtcaatacg tttggtgacc accgtatcgg catgatgaca gctatcgcag ccctcttggt
     8041 tgcagatgga gaggtggagc ttgaccgtgc agaagccatc aataccagct atcctagttt
     8101 ctttgatgat ttggagagct tgattcatgg ctaaggtgtt attagggttt atgggggctg
     8161 gaaaatcgac tattgcaaga ggcttggaca ctaattacct tgatatggat gctctgattg
     8221 agaagcgcct aggtatgtcc attgcgaatt ttttcgctga aaagggagaa gcagcctttc
     8281 gtcatgtaga atcagaagtc ctagctgatt tactacaaac agaccaagtc gtgtcaactg
     8341 gaggaggagt ggttatttct cagagaaatc gtgacttact caagactaat acagataaca
     8401 tctacctgaa agcagatttt gaaaccctct acctacgtat cgcagctgat aaggacaatc
     8461 agcgaccgct ttttctaaat aatagcaagg aagaactagt agctattttt caagaaagac
     8521 aggcttggta tgaggaagtg gctagtcggg ttttggatgt gaccaagcta agcccagagg
     8581 aaattataga ggaactaaga tgaaaattgc ttatctaggt cccaagggat cattttcaca
     8641 ccacgttgtg cagacagctt ttcctcatga ggaattgcag gcttttgaca acattacaga
     8701 tgtcatcaag gcttatgagc aaggattagt ggactattct gtggtgccag ttgaaaactc
     8761 tattgagggt agtgttcatg aaaccttgga ctatcttttt catcaggctc acatccaagc
     8821 agtagcagaa atcgttcagc ctattcatca gcagttgatg gtggttccag gccatactaa
     8881 gattgaaaag attttttcac atccacaggc cttggctcaa ggaaagaaat tcatcgatga
     8941 acaatatcca gaggctcaaa tcgaggtaac agctagtaca gcttatgcgg cccgttttat
     9001 ttccgaacat ccagatcagc cctttgcagc aattgcacct agaagttctg ctgaagaata
     9061 tggattggaa ctgattgccg aggatattca ggaaatggaa gccaatttca cacgtttctg
     9121 gcttctagga gctgaaaagc ctagtattcc cttgcaagca caaactgaaa agatgagttt
     9181 ggccttgaca ttacctgaca accttccagg tgcactttat aaggccctgt cgacctttgc
     9241 ttggcgagga attgacttga caaaaattga aagtcgtcca ctcaagacag cactgggtga
     9301 atactttttc attatcgatg tggattatac cgataaggac ttggtccact ttgcccaaaa
     9361 agaattagaa gcgattggaa tccagtataa aattctgggt gcctatccta tttatccaat
     9421 atcagaccat ggaaaggaga gaagatgagt aaagaaaatc ccttaagtca tcatgagcag
     9481 ttgcgttatg attatttgct aaaaaatatt cactatctca atgagagaga aaaaaatgag
     9541 tttgtctatt tgcaagaaaa gctaactctt gctaggggaa atagtagctc tagcttggaa
     9601 caagaaagag aagagcaggt tgacttacca agttatgcga accggagtcg ctcacaatcc
     9661 aaatcacaag cactctcttt ccctccaaaa aagaaaagac ggaagctccg tcttaaacga
     9721 atttttatgg tgattttttc acttttggtc tgtgtggctt tggccatggt attcatgttt
     9781 ttgcgtggtt accaagatgc tagcgcaaag aaaactgctg atgcccgggc agctcaagta
     9841 gaagtcttta atggtcagga cactagagat ggagttaata ttttaatcat gggtactgat
     9901 ggtcgaatcg gccagaacag tgttgagaca cgaactgact ctattatggt attaaatgtc
     9961 gggggctcag ataagaaaat gaagctagtc agtttcatgc gtgataattt ggtctatata
    10021 gatggttata gtcaagtgat taacggtaga aaacagacag ataacaagtt aaacgtagcc
    10081 tacgagttag gagaacaaga agggcaaaaa ggggcagaaa tggttcgcca agtcttgaaa
    10141 gataattttg acttggacat taagtactac gccttggttg atttccaggc ctttgccaca
    10201 gcgattgaca cgcttttccc tgatggggtg acgattgatg ctcaattttc aacattgaat
    10261 gggcgtcccc taacagaagc tacagtcgga gatgatttat acgctaccga gactgagtct
    10321 ccaacccaaa ccattaaagt cggaaaacag cagatgaatg gctcgaccct gctcaattat
    10381 gctcgtttcc gtgatgatga tgaggcggat tacggccgta caaaaagaca gcaacaagtt
    10441 ttaacagcaa ttttagagca aattaaagat cccactaaac ttttcactgg ttctgaagct
    10501 cttggaaagg tttttgctat gacctcaacc aacgtaccct atactttcct cctaacaaac
    10561 ggtttatctg ttttggatgg agcaaaaaat ggtattgaaa aattgacgat tccagaactg
    10621 ggtgactggg tagatgccta tgatgtttat ggaggcttgg gcttgctggt tgatcaaaac
    10681 aaatatcaaa ccaagctcgc tcaaatgggc ttgagataag ataatactca atgaaaatca
    10741 aagtgcaaac taggaagcta gccgcaggct gctaaaaaca cagttttgag gttgtagata
    10801 agactgacga agtcagttac ctatacctac ggcaaggtga agctgacgtg gtttgaagag
    10861 attttcgaag agtataagac agaaaaatca aagtttgata gctatttgga tagcagtcag
    10921 gctttgattt tttacagtct gcaatagatt ttcacccctt gtttgtggta taatgaaacg
    10981 atacgataga aagaataatg aacaatatga ctgatttaaa agcaattcag gctcgtagtc
    11041 tggagatggc tgaatatttt gtggcctttt gtaaagaaca tgatttgctc tgttatctct
    11101 gtggcggagg cgctattggt acccttcgaa acaagggatt tattccttgg gacgacgacc
    11161 tagacttttt tatgcctcgt aaagattatg agaaattagc agaattatgg cctcgttatg
    11221 cagatgaacg ttatttcttg tcaaagagtc acaaggattt tgttgatcgt aatcttttta
    11281 ttaccattcg tgacaagaaa accacctgta tcaagcctta tcagcaggat ttggatttgc
    11341 cacatggtct ggccttggat gttttgcctt tggattatta tccgaaaaat ccagctgagc
    11401 ggaaaaaaca ggttcgttgg gccttgattt attcactctt ttgtgcgcaa actattccag
    11461 aaaagcatgg tgctctcatg aaatggggaa gtcgcatttt actgggtttg actccaaaat
    11521 ctctccgtta tcgcatctgg aaaaaagctg agaaagaaat gactaagtat gatttggctg
    11581 attgtgatgg cattacagaa ttatgctcag gtcctggcta catgagaaac aagtacccaa
    11641 tcacatcttt tgaagacaat cttttcttgc catttgaagg aacagagatg cctattccaa
    11701 tcggctatga tgtctatctc agaactgctt ttggggatta tatgacgcct ccaccagcag
    11761 acaagcaggt accgcatcat gatgctgtca tcgctgatat ggataagtct tatacagaat
    11821 acaagggaga atatggtggc taagaaaaaa atcttatttt ttatgtggtc tttttctctt
    11881 ggaggtggtg cagagaagat tctatcaacc attgtttcaa atctggatcc agaaaagtat
    11941 gatattgata ttcttgaaat ggagcacttt gacaagggat atgaatctgt tccaaagcat
    12001 gtacgcattt taaaatccct tcaagattat cgccaaacca gatggttacg agcttttttg
    12061 tggagaatga gaatttattt tccaagactg actcgtcgtt tgcttgtaaa agatgattat
    12121 gatgttgaag tttcttttac cattatgaat ccaccactgt tgttctctaa aagaagagaa
    12181 gtcaagaaga tatcttggat tcatggaagt attgaagaac ttcttaagga tagctctaaa
    12241 agagaatcac atagaagcca gttggatgct gcgaatacaa ttgtagggat ttcaaaaaag
    12301 accagcaatt ctatcaagga agtttatcca gattatgctt ctaaattaca gacaatctac
    12361 aatggatatg attttcagac tattctagaa aaatctcaag agaagatcga tatcgagatt
    12421 gctcctcaaa gtatctgtac tatcggacgg attgaggaaa ataagggttc tgaccgtgta
    12481 gtggaagtga tacgattatt acaccaagag ggaaaaaact atcatctcta ttttatcggg
    12541 gctggtgata tggaagagga actgaaaaaa cgagtcaaag agtatgagat tgaggactat
    12601 gtacatttcc ttggttatca aaaaaatcct tatcagtatt tatctcagac gaaagttctc
    12661 ttgtctatgt ctaaacaaga aggttttcct ggagtgtatg tggaggcctt gagtctggga
    12721 ctccctttta tctctacgga cgttggaggg gctgaggaat tatcccaaga aggacgattt
    12781 ggacaaatca ttgagagcaa tcaagaggca gctcaggcga ttactaatta catgacttct
    12841 gcctcaaact ttgatgtcga tgaggctagc caattcattc aacaatttac aattacaaaa
    12901 caaatcgaac aagtagaaaa actattagag gagtagcatg gaaactgcat taattagtgt
    12961 gattgtgcca gtctataatg tggcgcagta cctagaaaaa tcgatagctt ccattcagaa
    13021 gcagacctat caaaatctgg aaattattct tgttgatgat ggtgcaacag acgaaagtgg
    13081 tcgcttgtgt gattcaatcg ctgaacaaga tgacagggtg tcagtgcttc ataaaaagaa
    13141 cgaaggattg tcgcaagcac gaaatgatgg gatgaagcag gctcacgggg attatctgat
    13201 ttttattgac tcagatgatt atatccatcc agaaatgatt cagagcttat atgagcaatt
    13261 agttcaagaa gatgcggatg tttcgagctg tggtgtcatg aatgtctatg ctaatgatga
    13321 aagcccacag tcagccaatc aggatgacta ttttgtctgt gattctcaaa catttctaaa
    13381 ggaatacctc ataggtgaaa aaatacctgg gacgatttgc aataagctaa tcaagagaca
    13441 gattgcaact gccctatcct ttcctaaggg gttgatttac gaagatgcct attaccattt
    13501 tgatttaatc aagttggcca agaagtatgt ggttaatact aaaccctatt attactattt
    13561 ccatagaggg gatagtatta cgaccaaacc ctatgcagag aaggatttag cctatattga
    13621 tatctaccaa aagttttata atgaagttgt gaaaaactat cctgacttga aagaggtcgc
    13681 ttttttcaga ttggcctatg cccacttctt tattctggat aagatgttgc tagatgatca
    13741 gtataaacag tttgaagcct attctcagat tcatcgtttt ttaaaaggcc atgcctttgc
    13801 tatttctagg aatccaattt tccgtaaggg gagaagaatt agtgctttgg ccctattcat
    13861 aaatatttcc ttataccgat tcttattact gaaaaatatt gaaaaatcta aaaaattaca
    13921 ttagaacggg ggtgaccaag tgattgatgg gaaacgatta ttatttagtt tgaccatagt
    13981 cagttatgcc ttgacgctag taagtggaat tgtgtatctg tttaataata ataatgttag
    14041 cttactttct actttattgt tcttgttggt tagtagcttg attgcttgtt ggaatg
//
LOCUS       DAVKJD010000045        13899 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKJD010000045 DAVKJD010000000
VERSION     DAVKJD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.045"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            8..1009
                     /locus_tag="VW157_001366"
     CDS             8..1009
                     /locus_tag="VW157_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9552658.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     misc_binding    1221..1450
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1617..1694
                     /locus_tag="VW157_001367"
                     /pseudo
     CDS             1617..1694
                     /locus_tag="VW157_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2150..3511
                     /gene="trpE"
                     /locus_tag="VW157_001368"
     CDS             2150..3511
                     /gene="trpE"
                     /locus_tag="VW157_001368"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9552659.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            3508..4074
                     /locus_tag="VW157_001369"
     CDS             3508..4074
                     /locus_tag="VW157_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9552660.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            4085..5089
                     /gene="trpD"
                     /locus_tag="VW157_001370"
     CDS             4085..5089
                     /gene="trpD"
                     /locus_tag="VW157_001370"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9552661.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            5086..5853
                     /gene="trpC"
                     /locus_tag="VW157_001371"
     CDS             5086..5853
                     /gene="trpC"
                     /locus_tag="VW157_001371"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9552662.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKKLYDYATELGLEVLV
                     ETHNLAELELAHRLGAEIIGVNNRNLTTFEVDLQTSVDLASYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            5840..6439
                     /locus_tag="VW157_001372"
     CDS             5840..6439
                     /locus_tag="VW157_001372"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9552663.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPVDVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            6417..7640
                     /gene="trpB"
                     /locus_tag="VW157_001373"
     CDS             6417..7640
                     /gene="trpB"
                     /locus_tag="VW157_001373"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9552664.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDMTGQDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            7633..8409
                     /locus_tag="VW157_001374"
     CDS             7633..8409
                     /locus_tag="VW157_001374"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9552665.1"
                     /translation="MPKTLTEKLNAIKVTGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGELIQDFIKQAVAYQK"
     gene            complement(8381..8482)
                     /locus_tag="VW157_001375"
                     /pseudo
     CDS             complement(8381..8482)
                     /locus_tag="VW157_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            8767..9441
                     /locus_tag="VW157_001376"
     CDS             8767..9441
                     /locus_tag="VW157_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552666.1"
                     /translation="MKGQKDYVDERLEKRETMIVSNGYAWTRPNHIDTAFASADLFEY
                     KLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFPLVQKNKSGYLFEYAQMNTL
                     YLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSNIEKFMILTYEADSENNII
                     SVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDIPNFSELSETEDFEIIPRIE
                     KQEGQK"
     gene            9456..10607
                     /locus_tag="VW157_001377"
     CDS             9456..10607
                     /locus_tag="VW157_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEU9552667.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(10659..11318)
                     /locus_tag="VW157_001378"
     CDS             complement(10659..11318)
                     /locus_tag="VW157_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9552668.1"
                     /translation="MIDIYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLSIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            11388..11834
                     /locus_tag="VW157_001379"
     CDS             11388..11834
                     /locus_tag="VW157_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9552669.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYIMKKGR"
     gene            complement(11906..12109)
                     /locus_tag="VW157_001380"
     CDS             complement(11906..12109)
                     /locus_tag="VW157_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9552670.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKTIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLHKEEK"
     gene            complement(12140..12748)
                     /locus_tag="VW157_001381"
     CDS             complement(12140..12748)
                     /locus_tag="VW157_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9552671.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            complement(12787..12957)
                     /locus_tag="VW157_001382"
     CDS             complement(12787..12957)
                     /locus_tag="VW157_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9552672.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            complement(12969..13535)
                     /gene="amaP"
                     /locus_tag="VW157_001383"
     CDS             complement(12969..13535)
                     /gene="amaP"
                     /locus_tag="VW157_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9552673.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(13619..13783)
                     /locus_tag="VW157_001384"
     CDS             complement(13619..13783)
                     /locus_tag="VW157_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9552674.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
BASE COUNT         4363 a         2490 c         3013 g         4033 t
ORIGIN      
        1 gactgaaatg aaaaatataa atgggaaaaa ggtaactatt tatgatattg cacgtttatc
       61 aggtttttct ccaaaaacag tttcacgagt tattaatggt ggagtaaatg ttaaagaaga
      121 aacttatcaa gcgattcaaa aagttataga ggaattgtcg tatattccaa atgcgtatgc
      181 aaaaaattta actaaaaaag aagctataaa tatattgatt tctgtaaaaa aaatcgactc
      241 ttttccttta atttggtttc atacgctgtt agataaagtg ttacggacgt gtaaagaatt
      301 tggtgttaat gctatagttg agtactttgg agaggaggat acaattagta attcgattat
      361 atcaagcaca ggtagcctag tagatggtgt tattgttttt tatgaaagtg tagatgatat
      421 cagaattcag tatttaaaga agaaccatat gccttttctt gttttcggtg aatctcaaac
      481 gtctggagta gtctatgtat ctaataataa ttttcaagct acttatgata tgatgaaagc
      541 tgtaactgaa gaaaaattta aaaatatgtg gttgcttatg ggaggagaat cccatgttaa
      601 taaggatcgt gaaagaggtg tgcgttcttt tctgaatgat aaaaattact ttatggattt
      661 aaaagtcatt tatggcttat ctacaattga ttcagtttat tcttatgcaa tgcagcactt
      721 gactacccaa aattatccgg acattatttt tgtttcagga gatgaaaagg ttcagggctt
      781 gattcgtgcg tgctatgaga aaggaattct tattccagat gatatttcta ttattggatt
      841 tgataatatt cctatttctc agtattatac tccagctttg tctacaattg ctcccaatta
      901 tgttaaattg gctaaagaaa tgatagaagg tgttttagca attattaaag gggaaagtgt
      961 cacatctgtt gaagtttctc ctaaatttgt taggagacag agtttctagt attgtttaat
     1021 tcttgaaaat cctagtggaa aagatggagt gttctaaaat aagttctgtt tagcctccat
     1081 tcagtttcta ttgagttgga tccaatttct aacaagtggg acatgttcat gttcgatcta
     1141 aggaagacat ggtcacattc gaatgatatt ttcaaaaaaa tcgtaaaaaa gtcttgacaa
     1201 agaaaaaaca aagtattaaa ataagttcaa caaatcagaa aagtaactat ttttgatctt
     1261 cagggagcct gtggtggttg tgaataggtg gttggaagta gtgaaagtgg gctgattttg
     1321 aaaatgaatt tgaaacaatg aaaattcggt gcgcacacct tacagtgcag cttgttgtta
     1381 aacaaggcag agatgtaaag ggatagtccc tttataattg aggtggcacc gcgttaccaa
     1441 cgccctcaca cggaagtata ttctgtgtgt gggctttttt ctatccgtcg tttggtttat
     1501 cttttattag ggccttaagt cgctttgatg aacttgagtt ctatctacag ctccttgcct
     1561 actactaaaa gcaaacaaaa aggctggact aatacgaaaa aagctagtaa tactcaatga
     1621 aaatcaaaga gcaaactaag aaactagccg caggttgctc aaaacactgt tttgaggttg
     1681 cagatagaac tgacgaagtc agtaaccata cctacggcaa ggcgacgttg acgcggtttg
     1741 aagagatttt cgaagagtat aaaggtgtac cgagatgaac atctcttcat caatgtctct
     1801 ggtcgtggag gcaaggacct agctgcgatt gcagattact agaagctaaa aaataataga
     1861 tggaaaaatt acagttcttt ctctcacaga gagcttgtgg ttgctgaaaa caagcagaga
     1921 aagctgtaaa gttgggttca tctgaaacga gagaagaaaa catcattctc aagggagtgc
     1981 cctttatcgc acagcctgtt acaggagata tctgagacag gaatgagaga taggagaaat
     2041 cctataattg aggtggcacc gcgaatttcg tcctcacgca agttattttg cgtggggatt
     2101 tttcatacag tcaccacgga ctagaagtag aactgaaagg gaaattacaa tggaacgaat
     2161 cattcatgga gatgtcttat caccaatctt ggcttatatg cgcttaaagg ggcaacataa
     2221 ggttatttta gagagtattc cgagagacaa ggaaacagct cgtttttcta tcctagccta
     2281 taatccagtt tttgagatta agtttgaaaa tggggttctc tatcaaaatg gtcaagtgat
     2341 tgatcgggat cccttggatt tcctttatga agtgattcat aagagtcagc accactcaga
     2401 gctacctttt ggtggtggag ccattggttt tgtgggttac gatatgattt cgctttatga
     2461 agaaattggt caaatccctg aggatacaat tgggacgcca gacatgcatt tctttgtcta
     2521 tgagagctat atggtctttg accacaagaa ggagaagata catgtcatag aggatgcact
     2581 ttatagtgag cgcagtcaag aggccttgga aaaatccttg aaccaagtgc ttgaggaatt
     2641 acgcattcct gctccaaatg aatttgaaga tttggatcta tctccgttag acttcaaacc
     2701 gcatatcgct ccacataagt ttgagggaat ggtagaaaca gctcgtgact tgattcgtaa
     2761 cggagatatg ttccaatgtg tactcagcca gcgtttttca gcagaagtta ctggaaatcc
     2821 atttgacttc tacagaaatc tccgcgtgac taatccttct aattaccttt atttctatga
     2881 ttttggggat tatcaaatca tcggagctag tccagaaagt ttggtttctg tcaaaaatgg
     2941 catcgtgaca accaatccga ttgcagggac gcgaccaaga ggggctacgg atgaagaaga
     3001 caagaccttg gcgactgacc tcctctctga tgagaaggaa acagcagaac atcgaatgtt
     3061 ggtagacttg gggcgtaacg atattggccg catctctgaa acggccagtg tccaagttac
     3121 taagtatatg gaggtggagc tcttccgcta cgtcatgcat ttgaccagtg tggtcaaggg
     3181 acgtttgctt gcagaactca ctgccatgga tgccttgaaa gcaacgcttc ctgctggaac
     3241 cgtttctgga gcaccaaaaa ttcgagcgat gagacgcatc tatgaactgg aaacggaaaa
     3301 acggggcgta tatgcaggag caatcggcta cttgtctgcg acgggtgata tggatttggc
     3361 cattgccatc cgaaccatga ttctcaaaaa tcaaagagcc tatgtgcagg ctggggcagg
     3421 gattgtctac gactctatcg cccaaaacga ataccaagaa accattaaca aagcaaaatc
     3481 tatgactaga attggagaac taagaccatg attttattga ttgacaacta tgattctttt
     3541 acctataact tggcccaata cattgggaat tttgcagaag ttcaggttct gagaaatgat
     3601 gattccaagc tgtatgaaga agctgaaaaa gcagatggtc tggtcttttc tcctggtcct
     3661 ggttggccag ttgatgctgg aaagatggaa gacatgattt gtgattttgc aggcaagaag
     3721 ccgattctag ggatttgttt gggtcaccaa gccattgcag aagtctttgg tggtaagcta
     3781 ggtttggctc caaaagtcat gcatgggaaa cagagcaata tcaactttga agcgccatct
     3841 gttttgtatc aaggtattga ggatggacgt gcggtcatgc gttatcacag tattttgatt
     3901 gaggaaatgc cagaagactt tgaagtgaca gctcgttcga ctgatgacca agccatcatg
     3961 gggattcaac ataaaaacct accgatttat ggcttccagt accatccaga gagcattgga
     4021 acgccagacg gcttgtcttc tattcggaat tttatcgaga aggttgtaaa gtgaggaaac
     4081 taggatgaaa gagattattg aaaaactagc aaaatttgaa aatttatcag gtgtggaaat
     4141 gacggatgtc attgagcgta tcgtaactgg gcgtgtaact gaagcgcaga ttgcttctct
     4201 cctcttagct cttaagatga agggggaaac acctgaagaa cgcacagcca ttgcccaagt
     4261 catgagagga catgcccagc atattccaac tgagattcat gatgccatgg acaactgtgg
     4321 tacaggtggg gacaagtctt tcagttttaa tatttccaca actgcagcct ttgtcttggc
     4381 tggtggcggt attcacatgg ccaagcatgg taaccgctcg atttcttcta aatctggttc
     4441 cgcagatgtc ctcgaagcct tgggaatcaa tcttgacctc aaaccagctg aactaggtaa
     4501 ggtctttgat aaaactggca tcgtctttct cttcgctaaa aatatgcacc cagctatgaa
     4561 atacatcatg ccagctcgtt tggaattggg aattccaacg attatgaact tgactggtcc
     4621 actgattcat ccaatggctt tagaaacaca gcttcttgga attagtcgtc cagaactcct
     4681 agaaagtaca gctcaggttt tgaaaaatat gggtcgcaaa cgtgccatcg tggttgctgg
     4741 accagaaggg ttggatgaag ctggcttgaa cggaacaacc aagattgcac ttcttgaaaa
     4801 tggcgaaatc agcttgtcaa gctttactcc agaggatttg ggaatggaag gctatgctat
     4861 ggaagatatt cgtggtggga atgctcagga aaatgcagaa attttgctta gcgttctgaa
     4921 aaacgaagca agtccattct tggaaacgac agtcttgaat gctggtcttg gtttctatgc
     4981 taatggtaag attgatagca tcaaggaagg agttgccttg gcccgtcaag tgattgctag
     5041 aggcaaggcc cttgaaaaac tcagactgtt acaggagtac caaaaatgag tcaggaattt
     5101 ttagcacgaa tcttagagca gaaggcgcgt gaggtggagc agatgaagct ggagcaaatc
     5161 cagcctctgc gccagaccta tcgcttggca gaatttttga agaatcatca ggaccgcttg
     5221 caggtaatcg ctgaggtcaa gaaagctagc cctagtttgg gagatatcaa tctcgatgtg
     5281 gatattgtgc aacaggccca gacttatgaa gaaaacggag cagtgatgat ttcggtgttg
     5341 acagatgagg ttttctttaa agggcatttg gattatctac gggaaatttc cagtcaggta
     5401 gagattccga cgctcaacaa agactttatc atagatgaaa agcaaatcat ccgcgctcgc
     5461 aatgcaggtg cgacagttat cttgcttatt gtggcagcct tgtccgaaga acgcctcaag
     5521 aaactgtatg actacgcgac agagcttggt ctggaagtct tagtggagac tcacaatcta
     5581 gctgaactag agctagccca cagacttggt gctgagatta tcggggtcaa caaccgcaac
     5641 ttgactacct ttgaggtcga cttgcagacc agtgtagatt tagcctctta ctttgaggaa
     5701 ggtcgctatt acatttctga atctgccatt ttcacagggc aggatgcgga acgactagcc
     5761 ccatacttta acggaatttt ggtagggaca gctcttatgc aggcagaaaa tgtggcccag
     5821 agaatcaagg agttgcagat tgacaaaggt taaaatttgt ggactatcga ccaaagaagc
     5881 ggtggaaaca gccgtttcag caggagccga ctatatcggt tttgtctttg cacctagtaa
     5941 aagacaggtg actttagaag aggcagctga gttggcaaag cttattcctg tagatgtgaa
     6001 aaaggttgga gtatttgttt caccaagtcg ggtagaactg ctggaagcga ttgacaaagt
     6061 tggcttggac ttggttcaag ttcacggtca ggtggcagat gatttatttg aaaatttgcc
     6121 ttgtgccagt attcaggctg tgcaggtaga tggaaatggg catgtcccta attctcaggc
     6181 agattatcta ctctttgatg cccctgtggc aggaagtggc cagccctttg attggggtca
     6241 actggatacg actggactag cacagccctt ctttatcgca ggtggcctta atgaagataa
     6301 tgtagtaaaa gcaattcaac attttactcc ctatgcagta gatgtatcga gcggagtgga
     6361 gacagatgga caaaaagatc atgaaaagat tagaagattt atagagaggg taaagcatgg
     6421 catatcagga accaaataaa gatggatttt acggaaaatt cggcggacgt tttgtcccag
     6481 aaacattgat gacagcagtt ttggagttgg agaaggccta ccgtgaaagt caggcagacc
     6541 caagtttcca agaggaatta aaccagcttt tgcgccagta cgtaggacgt gaaactcctc
     6601 tttattacgc aaaaaacttg acccagcata tcggcggagc caagatttat ctcaaacggg
     6661 aagaccttaa ccatacagga gcccacaaga ttaacaatgc cttaggacaa gtttggcttg
     6721 ccaaacgcat gggtaaaaag aaaattatcg cagaaacggg tgctggtcag cacggtgtgg
     6781 caactgcaac tgctgcggcc ctctttaaca tggaatgtac catctacatg ggtgaggaag
     6841 atgtcaaacg ccaagccctc aatgtcttcc gtatggagct tttgggagct aaggtcgagg
     6901 ccgtgacaga tggttcgcgc gtgctcaagg atgcggtcaa tgcagccctt cgttcatggg
     6961 tagctaatat cgatgatacc cactatatcc ttggttctgc cttggggcct catccatttc
     7021 cagaaatcgt tcgtgacttc caaagtgtca tcggtcgaga ggctaaacaa cagtaccgcg
     7081 atatgacagg tcaagatctg ccagatgccc tagtagcctg tgttggtggt ggttcaaatg
     7141 ccatcggtct cttccatcca tttgtagaag atgagtcagt agccatgtat ggagctgaag
     7201 cggctggact tggtgtggat acggagcacc acgcagctac cttgaccaag ggtcgtccag
     7261 gtgtccttca cggttccctc atggatgtgc tccaagatgc ccatggtcaa attcttgaag
     7321 ccttctctat ctcagcaggt ttggactatc ctggtatcgg tccagagcat tctcactacc
     7381 acgatattaa acgtgccagc tatgtccctg tgacagacga agaagccttg gaaggattcc
     7441 aactcttgtc tcgtgtggaa gggattatcc cagccttgga atctagccat gctatcgcct
     7501 ttgcggtgaa attggccaaa gaacttggac cagaaaagtc tatgattgtc tgcctatcag
     7561 gtcgtgggga caaggatgtg gttcaagtca aagaccgctt ggaagcagat gcagcaaaga
     7621 agggagaagc tcatgcctaa gacactaaca gaaaaattga atgctataaa agtaactgga
     7681 aaaggaattt ttgttcccta tatcatggct ggtgaccacg aaaaaggttt ggacggtctt
     7741 gctgaaacaa ttcacttttt agaagatttg ggtgtctctg caattgaagt gggtattccc
     7801 ttttcagacc ctgttgcaga tggacctgtt atcgaagaag ctggcttgcg cagtttagcc
     7861 cacgggacct ctacccaggc tttagttgaa accttgaaaa ccattgaaac agagattcca
     7921 ctggtcatca tgacctactt caaccccctc tttcagtacg gtgtggagaa ctttgtcaaa
     7981 gatctggctg atacagctgt gaagggcttg attatcccag acctgcctca tgaacatgcc
     8041 aactttgtag aaccattttt ggcagataca gatatcgcct tgattccttt agtaagcttg
     8101 accacaggaa ttgagcgcca gaaagagttg attgaagggg cggagggatt cgtctatgcc
     8161 gttgccatca atggggtaac agggaaatca ggcaattacc gtgcagattt ggacaagcac
     8221 ttggcgcaac ttcatcaagt ggccgacatc ccagtcttga caggttttgg tgtatctagt
     8281 caagctgatc tggaacgctt caatgcggtg tcagatggtg ttatcgtcgg ttcgaaaata
     8341 gtaaaagctc tccaccaagg agagctgatt caggacttta tcaaacaagc agtagcttac
     8401 caaaaataat caaacaagta gcaagtaaga ggaaaggcac gaaaggaagt ctttcctttt
     8461 tcttttgcag gagaaaggct agttttatag tgaattgaat ctggaatatt acactacggt
     8521 tcctaaaata ttttcagaaa gcgatttgaa tttcccaata aatttgttca gtttttaatt
     8581 gattttacta tagaagatat aattttctaa attactctga cacagaactt taagaaaaag
     8641 ctaattattg tagtaatttc tttaagaaaa tgatataatt cattaaaaag tatttggagg
     8701 aggaagaatt atgttgaatc agatctcttt gattcgcttg aggcacaaaa aattgtagat
     8761 actttgatga aagggcaaaa agattatgta gatgaacgtc tagaaaaaag agagacaatg
     8821 atagtgtcga atggttatgc atggacgcga cctaatcata ttgatactgc atttgcatca
     8881 gcagatttgt ttgagtataa attacaatta gcaggacaga cttggggata tttagaattt
     8941 gaaacaaata cagaaaaata tgggaaagta ttgttaatta taaagggtaa gaagcgactt
     9001 acgaaccaat ttcctttggt acaaaaaaat aagagtggct acttatttga atacgctcag
     9061 atgaatacac tttatcttaa tcaacattct tcctacaaaa atgatgaaga tagtcattcc
     9121 tttccaattc agatggagtt agtttctgat gaaatgattc aagaaattga acaagctact
     9181 aaaaattcga atatcgaaaa atttatgatt ttaacttatg aggcggactc agaaaacaat
     9241 attatatctg tagatgttgt tatgcctgat gcacgaactg gtcagttaca cttgattcaa
     9301 gatttgtctg agtatattca atcaagttcg taccatttcg aagaagctaa atatcaagat
     9361 attcctaatt tttcagaatt atcggaaaca gaagattttg aaattattcc aagaatagaa
     9421 aaacaagaag gtcaaaagta gtaaggagta tgtaaatgtt taatggtcgg gtattgaaag
     9481 aattacggct gttaaatggt ttaagtagag cagaattagc tcagagaatt aatttaacgg
     9541 aacaagccat ttggcagttt gagtccaacg aaacgaaacc taaattatca accaaaatgc
     9601 atttggccaa ccaatttcat gttgatttaa cttattttga acaggaagaa gagagcattc
     9661 gatttgattc ttctgtaatt gcctttagaa atgcagacct agcaacacgg aaaacaatag
     9721 atattcaaac tatgtattta cataaggtag atagtttgat tgattatttt gaaagttttg
     9781 taattatacc taatattata attcatgacc taagtaatgt agtgagtgaa tcttatcata
     9841 agggagaatc cattgaggaa ttggctcttt atgccaggga aaaattaggt atttcaaaag
     9901 ataatcatga tttgctttat aaattagaac gttcaggcat ctatatcgtg gaacgattaa
     9961 ttaatggcca agctgatgct tatagcgcat ggtcaaaatt gggaagacct tatattgtgt
    10021 tgggaacgaa taaatcatct gtacgtcgaa attttgactt agctcatgag ctaggacata
    10081 ttcttttaca taaatataaa gatatgaatg aagatggcga tcgtttggag caagaagcaa
    10141 attattttgc atcatgtttt ttattgccaa aagaagagtt tttagtcaaa tttgaagaga
    10201 gggttggcaa gcgtgtcagc aatcctgata gttatatttt attgaagtcg gatttgaatg
    10261 tttcgataca ggctttagag tatcgagctt ttaagttagg attattgact ccaaagcaac
    10321 attcttactt ttatcgtcaa attgcgcaaa aaggttacaa aatgattgaa cctttggatg
    10381 atcaaatttt tgttaaaaaa ccaagcaaag taaagagtat tctggacgtc gttttgagta
    10441 atcatctagt cagtctagcg actataatgt ctaaacaaag tattcgttta cagtttataa
    10501 gcgaaatatt ttcagtcgaa atgaaatttt ttgatcagta tcaagaagat agaagaacag
    10561 atcgatttga taacatcatc cctttgtaca aaagaaataa tttataattt aactgtgaaa
    10621 taatttcatg aaggacatat ggcagaaatt ggactttatc aaacaagcag tagcttacca
    10681 aaaataatca aacaagtagc aagtaagagg aaaggcacga aaggaagtct ttcctttttc
    10741 ttttgcagga gaaaggccag gatacccgtc gcagaagcga actgaatcaa gatcagtaac
    10801 tccgttacgc taaagacgag agcacaagaa gctaaaaaaa ggaaatcccc tgcacccatg
    10861 cggatatcga taaaatgagc caaaattcca agagcaagga aggagaccat gaccagattc
    10921 cagccagagg aagctattag gattaggtgg aaagtcatcc agaccagtaa gggatattcc
    10981 tgatggcgaa agtcatagat gctcaaggtc aaaccagcgg tgattaggac gacttgcccc
    11041 aaggaaagca attcccaaga gtaaagcaga aagaggagtc ctaagcctag ttcaaagagg
    11101 gcataccaga caggatagcg gaccttgcag tagcgacagc gaaagcgatt gaagacctgt
    11161 gagagaatcg gaatcaaatc taagggacgc aagggagtct gacaggaatc gcagtgactg
    11221 gctgaactga taatggattg ctctggaaaa cggtcaatga ccaaaccaag aaaggaagcg
    11281 agaatgctcc cgacaagaaa aaaataaata tcaatcatac ttatctattc gtaaaaaatg
    11341 ggagaagtag tataatggag aaacatagat aagatggtga ggaaaagatg acaattcgtt
    11401 ttgaagaaaa ggtgagtata gaaaacgctc agctcgtatg ccaatggtcc aactccttag
    11461 gcaaagtctt tcaagaacaa tggatgggac caaggattcc ttttctactg acccttcaag
    11521 ccttggaagg agtcttttca atctttgatg aacaagagtt tgtgggactt atccagaaaa
    11581 tcaggctaga agacagcaat cttcatatcg ggagattttt tatcaacccc cagaagcagg
    11641 agcaagactt aggtagccag gctttaagga aatttgttag tttggccttt gaaaatgaag
    11701 atatagatag tatttctcta aatgtcttcg aggcaaatca aagagctcag aatctttacc
    11761 aaaaagaagg atttgaaatc gttcaaatgg ttgaagcacc tgtaagaaaa tacatcatga
    11821 aaaaggggag ataaacatca aaaaagcctt gattccaagg cttttttgat tttttggtct
    11881 taaaagaata tataaagtct aaaccttatt tctcttcttt atgaagcatg tttttaacac
    11941 cttctacagc accttctaca gcgtctttgg catcttcggc gacttctttt actttagaaa
    12001 caactttttc agctgctcct tctttttcca ttttttcatc accgatggtt ttcccaacac
    12061 cttctttaat agaaccttta gcttgattta atttttcttc tactgacatg ataatttctc
    12121 ctgttatatt tattttatct tagtttacac gagtgttttc gtgagatact acaccatttg
    12181 ctgcaccttt aacagcttct acaccttcgc taactttttc ttgaacagtt gagaaaccag
    12241 atccaagacc agatttagct ttttcgaatt gttctgaagt gaattctcct gttgattcag
    12301 caacgtcaga tacgcgatct tgaaggctta ctgagtctgc ttcatgctgt tctttagttt
    12361 tgatgtcgac aacgtttacg ttgatttcaa caatttccaa gtcagtcatt ttagcaactt
    12421 ctgaagatac gatttctctg atttctgaat ataaagctgg aacatttttt tggtactcaa
    12481 caataacgtt taagtcaact gcaacttgtg ttttaccaac ttctacgtta acaccacttg
    12541 ttacgtcatc gctgttaacg attttttctt taagatttga gaagaaaccg ccatcgattc
    12601 ccaaaagacc tgaaacgttt tctagtgaaa gaccaatgat tttttggata actttatctt
    12661 cgtaagtaag ttcccctttg atttcgtgag ctacaacagt agcatctttc ttttctacgt
    12721 tagtgtttgt gtttttttcg tttgacatat gaaactcctt taattaaatt agtaattttt
    12781 atttttttat ttatctatat agtttttttc gatatataat ccagctgcaa ctcccagtgc
    12841 tcctaaaatc aatacaaata ttgttttgaa gaagccaaag gagacaatca aacaagccag
    12901 aaatacgcct atgagaccag cgataattgg atatcgatat tgtttaagcc attccatttt
    12961 ttacttcctt acttcacacg actaacagtc tttttgtttt gaggttttgg ttggtaattt
    13021 tttactgcaa cttcaagttt tacttgacgc tcaataccaa aaaactgctt caatccatta
    13081 gttatttcat tttgaattat ttggcatctg tcagcgatgt tgtctgaagg aagaatttta
    13141 ccttctacat gaacgaaaca tttatttttt cgtaaattta catgaacagt tgggttcttg
    13201 atcaatctat gatcactcac caaacttcta acaaaacctt cgattgccga attctttaat
    13261 tttaatgtat cgtttttagt ttcaagttgg atttccaagt aacgtttagg ataaaacatc
    13321 acaactaaaa tggataataa aactaaagtt gatagaacca ctgtccccca aaagacatat
    13381 ctagcaagat agaattcaac tacggagttc tgtctccagc taagtagatg aatacctaga
    13441 tcactaactt gatgataatc tatcaaaaca ggaaggaaaa ttgtcaagat taaaatacag
    13501 aaaataatga aacatatttt ctttgctttt gacatataca atcctttcga tttaacattt
    13561 aaactatttt tcccgtcatt gatttaaaaa tagtttgcta actttactgg cgaaaagttt
    13621 actttctacc tgtaaagaat gacactacag taatcacaat cgctgcacct acaatagatg
    13681 ggagcaaagc cattccagcg attgatggac cccaactacc taaaagagat tgtcctgcat
    13741 atgccccgac taaaccagcg aatacatttg cgatgattcc catagaacta cttttttagt
    13801 gattttacct gccaagagac caataagacc tccagcaata attgatcaca acactgccta
    13861 ccctcctttt ttattttaat atcaaaaaat caaattctt
//
LOCUS       DAVKJD010000046        13626 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKJD010000046 DAVKJD010000000
VERSION     DAVKJD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13626
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.046"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(138..575)
                     /locus_tag="VW157_001385"
     CDS             complement(138..575)
                     /locus_tag="VW157_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9552675.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(660..3293)
                     /gene="polA"
                     /locus_tag="VW157_001386"
     CDS             complement(660..3293)
                     /gene="polA"
                     /locus_tag="VW157_001386"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9552676.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYLQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKSYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3544..3894
                     /locus_tag="VW157_001387"
     CDS             3544..3894
                     /locus_tag="VW157_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9552677.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWTYLCSQVN
                     QNLEKIRQREGDTH"
     gene            4109..4225
                     /locus_tag="VW157_001388"
     CDS             4109..4225
                     /locus_tag="VW157_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9552678.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            4218..4451
                     /locus_tag="VW157_001389"
     CDS             4218..4451
                     /locus_tag="VW157_001389"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552679.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            4578..4859
                     /locus_tag="VW157_001390"
                     /pseudo
     CDS             4578..4859
                     /locus_tag="VW157_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4993..5961)
                     /locus_tag="VW157_001391"
     CDS             complement(4993..5961)
                     /locus_tag="VW157_001391"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9552680.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(6106..6921)
                     /locus_tag="VW157_001392"
     CDS             complement(6106..6921)
                     /locus_tag="VW157_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEU9552681.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPD
                     STFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLI
                     FLGLALGTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(6946..7443)
                     /locus_tag="VW157_001393"
     CDS             complement(6946..7443)
                     /locus_tag="VW157_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9552682.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(7516..8877)
                     /gene="radA"
                     /locus_tag="VW157_001394"
     CDS             complement(7516..8877)
                     /gene="radA"
                     /locus_tag="VW157_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9552683.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDREFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(8891..9406)
                     /locus_tag="VW157_001395"
     CDS             complement(8891..9406)
                     /locus_tag="VW157_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9552684.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(9408..9851)
                     /locus_tag="VW157_001396"
     CDS             complement(9408..9851)
                     /locus_tag="VW157_001396"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9552685.1"
                     /translation="MKIRGFELVSNFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(9908..10005)
                     /gene="ffs"
                     /locus_tag="VW157_001397"
     ncRNA           complement(9908..10005)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW157_001397"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(10038..10505)
                     /locus_tag="VW157_001398"
     CDS             complement(10038..10505)
                     /locus_tag="VW157_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9552686.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(10706..11992)
                     /locus_tag="VW157_001399"
     CDS             complement(10706..11992)
                     /locus_tag="VW157_001399"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9552687.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(12223..12459)
                     /locus_tag="VW157_001400"
     CDS             complement(12223..12459)
                     /locus_tag="VW157_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9552688.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            12724..13521
                     /locus_tag="VW157_001401"
                     /pseudo
     CDS             12724..13521
                     /locus_tag="VW157_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4304 a         3258 c         2293 g         3771 t
ORIGIN      
        1 ggtgttttga gctgacttcg tcagttctat ctgcaacctc aaaacagtgt tttgagcaac
       61 ccgcagctag tttcctagtt tgctctttga ttttcattga gtataacaca aaaggtagcc
      121 catcagctac ctttttctta tgcttcctca atcaagcgag tatgttctct cttgatacag
      181 cgattcatca cgatatcatc acatccacca tcacgcaaaa tctctttcgc ttctaaactt
      241 tcaagtccta gctgtgccca aaaaatcttg gcatcagctt tgagaaaatc acgcgccaca
      301 tcgggcagaa attcactgcg acgataaaca ttgacaatat ctacaggaaa aggaatttca
      361 gcgaggctag cataagcctt ttcacccaag atttcgccac ctgccgcctt gggattgact
      421 gggatgattt tatagccccg agcctgcatt tcctttgtta ctcgattact ggttgtttct
      481 tcacggtcag acaaacccac cacagcaagg gttttactcg ttgcgagata ctgacgaatc
      541 acgccatcac ttggattgat aaattcttga ctcatagaaa tcctcctttt tcatcagtat
      601 agcacatttt gaaaaggttt gcagaattct actacaaaaa aggaggacta gccccctttt
      661 tatttagcct cgtaccaggt tgccccttca ttctcatctg cgataagagg aacactgagt
      721 tgaatggctt cttccatggt ttgtttcacc aattttttca tctctaccaa ttcagattta
      781 ggcacttcaa ggacgatttc atcgtgcact tgtaacagca tcttggtctg ataaccacct
      841 gcaaccaagg ctttatccag ctgaatcatg gcaatcttga gaatatctgc tgccgaaccc
      901 tggataggtg agttgatagc agttcgctcc gcaaaaccac gaatattgaa gttgcgcgaa
      961 ttgatatctg gcaactcacg gcgacgctta aagagggtct ctacatagct cttatcacgc
     1021 gcctcccgca ccacttcatc catgtagttt ttaatacctg gaaaacgttc aaagtaggta
     1081 tcaatgtagg ctttggcttc cttacgacta attcccaaat tattagacaa gccaaagtct
     1141 gaaatcccat aaaccactcc aaagttaact gccttggcat tgcgacgatc atttgcagtc
     1201 acattatcag gacgctcaat gccaaagacc cgcatggctg tcgaagtatg gatatctgcc
     1261 ccctcttgga aggccttaat caagtgctca tccttagaaa tatgcgccaa aacgcgcaat
     1321 tcaatctgtg aatagtcaga gctgagtagc acactatcct cccactctgg cacaaaagcc
     1381 ttccgaatca agcgcccctg ttccaatcgg gcaggaatat tttgcaagtt tggatccaca
     1441 ctagacaaac gcccggtctg ggtcaaatcc tgcaaatagc gagtatgaat ctttccatca
     1501 gccaaaatcc agtcctgcaa gccaattaca taagtagatt gaatcttagc aatttgacgg
     1561 taatccagga ttttcttaac aatcggagca ataggagcga gacgctccaa aacatccact
     1621 gctgtcgaat aacctgtctt ggttttctta gtgtattcta gaggaagtcc caatttctca
     1681 aagagaagca cgcccaactg cttaggcgag ttgacattaa actcctcacc agccagctcg
     1741 taaatctctt gagtcagttt ttcaatgaca agctcatttt cagcctgcat ctcaagcaag
     1801 gtctctttct tgaccataat cccagcaatt tccatcttgg caaggacaaa agccagaggt
     1861 tgctccatat cataaagaag ctctaattgc ccattttcgc tgagtttttc aagtaaaata
     1921 ggctctgttt ctaccaaaac agcaagttta caagctaagt gttccaagaa tttctcacgt
     1981 tcaggaatgg cctttttaac acccttaccg tagaaagttt catcatcaac caagtaagtc
     2041 tgaccataaa gactagcgat ggtcgcaatt tcattgtcct ccacagtcga aaggaggtat
     2101 ttagccaaac ggatgtcaaa agcaggcgcc tgcaaatcca caccaaaacg ttgcaaaaga
     2161 actttaacct tcttaaagtc ataaactctc agagatgttt tttctaagaa atccttgaaa
     2221 atcgggtctt gcaacagctc aagcttgtct gtggcataga gcttatcccc acaagaccag
     2281 gcaaatccaa ccaaattatc cgtatggtaa ttctcaccaa aaagctcaaa gtggaagata
     2341 gactcttcac tcagcatatc ttgactgatt tggtcaacaa tagtaaaatc caaactctca
     2401 gacacatcag ctgacgacac atttaaagcc tgcttgagct gtttgaagcc catctcatcg
     2461 tagaatttcc caagattttc aacatctgga ccactataga ccaagtcctc taaaccaatc
     2521 gcaatcggtg ccttggtatc aatggtcgct agtgttttag acaaaaaggc ctgttccttg
     2581 tcattgatga gattttcctt catcttagaa gtcttcattc catcaatatt ttcataaatc
     2641 ccctcaagcg aaccatgctc cagcaagagc ttaatacccg tcttttcacc gactttggtc
     2701 accccaggga tattatccga cttatcaccc atgagcgcct tgagatcgat aaactgagct
     2761 ggtgtgaggc ccatttcttc catgaggtaa tctggcgtaa aggcctcaaa ctcagccaca
     2821 cctttcttgg aaatttcaac caccgtatgc tcatccgtca gctgaatcaa atccttgtcc
     2881 ccactgacaa tggtaatatc aaaaccatcc tgctctgcta gcttatccag cgtcccaatg
     2941 atgtcatccg cctcatactg agccagctca tagtgacgaa tccccatatg atccagcaac
     3001 tcacgaatga aaggaaattg ctcacgaaac tcatcaggag tcttggcccg accaccctta
     3061 tagtccgcat acatctctgt ccggaaggtc gtctttcccg catcaaaagc caccaaaata
     3121 tgactcggct caacccgctc caacaaatga ctcaacatca actgaaaacc ataaatcgca
     3181 ttggtatgca aaccagccgc attcttaaaa cggtccaact gctgatacag cgcaaaaaac
     3241 gcccgaaaag ctacagaaga cccatcaatc aataataatt ttttcttatc catacaccca
     3301 ttataaagga aagaatcaaa aaataccatt gggaagagct agagcaagta tttttcaaac
     3361 tttttccgaa taaatagata gagccagaga atttagtaaa cctagattta aaaatgtgct
     3421 ataatatatt gaatctataa tagtacacct tgactgctaa aatatttcta taaattaatt
     3481 tgactttcct gatagagtta ttcacatctt atttcaactc actatagaag gaggaatagg
     3541 aggattctca gacatccggg catcagccca actaatgatt tgattgctaa gaaaatattc
     3601 agcaatccag aaatcacttg tcaatttatt cgcgatatgc tggacttgcc agcaaaaaat
     3661 gtgaccattt tggagggaag cgatattcac gtattactct ccatgcctta ctcggtgcag
     3721 gatttttata ccagtataga cgtcttggcg gagttggata acggtactca agtaattatt
     3781 gagattcaag tccatcatca gaattttttc atcaatcact tgtggactta cctgtgcagt
     3841 caggttaatc aaaatcttga aaaaattcgt cagcgagaag gtgatactca ctagagctac
     3901 aaacacatcg ctcctgttta cgccattgct atcgtggata gtaattattt ctcagatgac
     3961 ctggcttttc atagctttag tatgcgcgaa gacacaacag gtgaggtatt ggcgattacc
     4021 aacaatggac aggaaaacca tctggttaag atggcattct tggaattaaa aaatacagag
     4081 aaaccagcaa ggacgaggtt cgcaagccat ggttggagtt tttcggcaac aagcccttta
     4141 cccagcaacc gcaacgagcc attacccaag caaatcaact gctggactac aagagctggt
     4201 ccgaggagga caggaaaatg tttagtcaac tacatatgcg agaagaacaa gtcttgttag
     4261 cacaggacta tgccttggaa actgctaggg ctgaaggcct tgaacaagga ctagagcgtg
     4321 ggaaagttga aggaagggca gaaaggaaac tttttgcctt cctagacata gtacgccaag
     4381 gtcttctgac ttctgaggtt gccagccagc aattaggtat gtcagtatct gaatttgagg
     4441 cactgttgta aaatggctcc ataatatcca taggggattt acccactatg gatattatgg
     4501 agcctatttt gtgtaaaaaa aaagtcccat atgacctata atgaaaagcg acaaaacaac
     4561 tcattagaaa gaatcatatg gaacaattac attttatcac aaaattacta gacattaaag
     4621 accctaatgt ccagatttta aacatcatca ataaggatac acacaaggaa atcatcgcca
     4681 aactggacta cgacgcccca tcttgccctg agtgcggaaa ccaattgaag aaatatgact
     4741 ttcaaaaacc ttctaaaatt ccttatcttg aaacgactgg tatacctact agaattctcc
     4801 ttagaaagcg tcgattcaag tgctatcact gttcaaaaat gatggtcgct gaaacttctg
     4861 atgacgtaca gtcatatttc ttctcttttt attatatcac agttttaaat ctagctttac
     4921 tagattcacc gctactatct atttattcgg aaaaaagacg aaaaaacctg agaatcatct
     4981 caggcttggt cattaaattt ttttctcaat atcgaaaagt ggagaaagtg gtcgtttttc
     5041 atgaatacgt acgatagcat cccctaggag atgagcgatt gaaatctgct caatcttatc
     5101 aatcaaacgc tcttctggca gatagatggt atccaaaaca accaatttct taatagctga
     5161 tttttggata ttgtccgtag caggaccaga aagaactggg tgcgtacagc ttgcatagac
     5221 ttcaacagca ccagcttccg caagagcatc tgccgcatga caaatcgttc cagcggtatc
     5281 aatcatatca tcaatcaaga tacaagtctt gccttcaacc ttaccgatga tgttcataac
     5341 ttcactagta ttcatcttat caacgctacg acgtttatca ataatagcga tagatgtttt
     5401 caaaaattct gccaacttac gagcacgagt cacccctcca tggtccgggc tgacaaccac
     5461 atagtcagaa ccaaccatac cacgacgctc aaaataatct gcaatcagag gagcacccat
     5521 caaatgatcc acaggaatat caaagaatcc ttgaatttgc gcagcatgca agtcgatggt
     5581 caataaacga tccactccag ctacttcaag catatttgcg acaagttttg aagtgattgg
     5641 ctcacgcgct ctcgcctttc tatcctgacg tgcataccca tagtaaggca tgacaacatt
     5701 gacagattct gcactcgcac gcttcaaagc atctaccata atcaaaattt caagcagatt
     5761 gtcatttaca ggcgaactag ttgattgtaa gataaagacg tgtttcccac ggattgattc
     5821 ttcaatgttg acctgaatct ctccatctga aaattggcga acacttgatt tccccaactc
     5881 tatcccaatc tcctgcgcca cacgttctgc caattcttta ttagaagaaa gggcaaacag
     5941 ctttaaatca gaaaaagaca tgatttcctc cggtatatat gtataacttg tgcctttcac
     6001 aagattttcc atctaccatt gtagcgcttt ttgcactatt tttcaatcaa aaataaaaga
     6061 agggcaccat atttgtaccc ttgcatcatt cttttgaaaa atattctagg tcatcaactc
     6121 attgtgtttc tcaacaaagc aataagcatg ataaaaacca tagagagcaa tagccgtaac
     6181 cactggaatc gctaaaggca actctgtttc caactccaca aaaggagagt taaacaagaa
     6241 gtgagttccc aaggctaaac ctagaaaaat aaggccctgt ttcttgccaa ccttctgtcc
     6301 tttataggct ctgtaaagca agtaaacacc tactacagct agacctgaaa aagtccagtg
     6361 agaggcaatt cctgagatga tacgctctaa aattcgcgaa atagtaaagt caaagccctc
     6421 tggcaaatcc gtacgaatat aaccaatatc ctcaatcatt tggaatccca aaccggaagc
     6481 aattccaagt aaaaacaaag attttaattt tcgcacagga atcaaagcca aaacaaaaac
     6541 aagtggcaat aatttcaagg gttcttctac caaaggagcc acaatagcac tttcaaaggc
     6601 atttaaaaat gtactatctg ggaaaagaac ccccagtaaa tcatggatat aagtattagc
     6661 aaaactagac aaccagcctg aaaggaacat ccctcccaat aaagacagaa tcaaaacctt
     6721 ctttggcaat tcccattttt tcccaatacg gaagagaaaa taaagagccg gaatcatgta
     6781 aaagagagct agaaagatag aaactcccat tagtccatat tccgcacctg acctcgaacc
     6841 gtccgtatag tagatggttt catactgtaa accaatacat agcaataaaa taaaaataaa
     6901 taaaatactg cttttcttca tacactttct ttctaaatga agtatttata attctacgac
     6961 tgtcatactt cctgtatcaa cattgtaaat agcaccagag ataatgacat cgtctggtat
     7021 taggggagac tcgataagca gttgcatatc ctcgcgtaca ctctcttcta tatcttggaa
     7081 gggcaagaag tcctggtctg acacatctac acctaattcc tcttttaaat actcctgaaa
     7141 aggttcattt tcaaaggtct gagcaccaca gtctgtatgg tgcaatacca caatctctct
     7201 tgtccccatt tgttgctggg aaataactag cgaacgaatc atgtcttcag tcactcgacc
     7261 acctgcattc cgcaaaatat gagcatcccc aagtgccaag cccagagctt gcgcaacgtg
     7321 caaacgtgag tccatacagg tcacaatggc tactctggtt ttaggtttaa gtggcagatt
     7381 taactgccca tgtagggcaa cataagcctg attggcttgc ataaactgtt caaaatacga
     7441 cacgattccc tccttgaaaa tttgatagtc aaatatttct cctatcttat catttttaag
     7501 agaatttgtc acggattatg caaagacctt tttcaaaact tcctgaatcg ttgtcacgcc
     7561 aatgacctga atttccttag gcagagtgat tcctgtcaag gaattcttag gtacataaat
     7621 cttagtaaag cccagtttag cagcttcgtt gatgcgttgc tcaatacgat tcacgcgccg
     7681 aatctctcct gtcaagccca gttctccgac aaaacattcc tgaggattag ttggcttgtc
     7741 tttgtagctc gaagcaatag caactgcaac agccaagtca atcgcaggtt catccaattt
     7801 aacaccacca gcagatttga gataggcatc ctgattttgc aagagaagcc ctgcccgttt
     7861 ttccaaaaca gccataatca agctagcacg gttaaaatca agtcctgtcg tagtacgctt
     7921 ggcatttcca aacatggtcg gtgttaccaa agcctgaacc tccgccaaaa tcggacgcgt
     7981 cccttccatg gttacaacga tggaggaacc agtcgctcca tccaaacgct cttctaggaa
     8041 aacttgactc ggattgagta cctcaaccaa gccgcccgac tgcatctcaa aaatcccaat
     8101 ctcattagtg gaaccaaaac gatttttgac cgctctcaaa atacgaaagg tgtggtgacg
     8161 ctccccttca aagtaaagca ctgtatccac catatgctcc aacatacgag gcccagccaa
     8221 ggttccttct ttggtcacat gacctacgat aaagatggca atgttattgg tcttggccaa
     8281 ctgcatgagt tcagcggtca cttcacgcac ctgagaaaca gacccctgca cccctgaaat
     8341 ctcaggagac atgatggtct ggatggaatc aataatgaga aagtctggct ggatacgctc
     8401 cacttctgca cgaacactct gcatattggt ctctgcatag agataaaact ctctatcaat
     8461 atcacctaag cgctctgcac gtagtttaat ctgctgggca gactcctccc cactgacata
     8521 gagaactgtc cccacttggg acaactgggt tgagacttgt aggagaagag ttgatttccc
     8581 aatcccagga tccccaccga tgaggacgag acttcctggt accactccgc ctccaagcac
     8641 acggttgaat tcctccatct ccgtcttggt tcgattgaca ttgatggaag tcacctcagc
     8701 tagtttcatg ggcttggttt tctcacctgt caaggacaca cgcgcattct tgacctcggc
     8761 aacctcaacc tcttccacaa aagaagacca agacccacag ttggggcaac gtcccagata
     8821 tttaggggaa ttatacccac aattttgaca tacaaatgtc gcttttttct ttgcgatgac
     8881 aaacctcttt ctatatctct aactcacact caatcacttg gcaaaaatca atcttctcat
     8941 ttggcacaaa ctggcgcatg agcattcgat gagcaacaac taccacagtc tgatgttctc
     9001 gatacttaga catacattct agaaaccgag acttcatttc cgtagctgtc tcatattgaa
     9061 taggactatt aggaagcaac tcccccttgt tttctaaaaa cagtcttcta gctgtttcaa
     9121 agttttctat tcctgtttta tagacctgcc attcatgtaa taaaggctct actcttaaag
     9181 gaagacccgt agcacagacc acatacgaag ccgtttctaa agctcttgtg actgcagaag
     9241 atacgattat ttcagctgac gagagtaaag gatttttgct caatttctgg acttgctgcc
     9301 gtcccatctc agacaagggt gccaaatcta tcccaaatcc tatataagaa cgctcctcta
     9361 actcacggta atctggctcc ccatgacgta caaagataat cttcattcta gtgccctgtc
     9421 gatccaaatc caccagttcg aacgccatca gccgcatctc catctgcaat taagaaagta
     9481 gcaaaaacag cctggacaat acgctcccca acttcaagaa caacctcttg gtctgtgata
     9541 ttcttcatct gcgcaaaaat atgcccttca tttccaggat ttccataata atctccatca
     9601 atgaccccaa ctgagttaat taaaaccaag cccttcttac gaggatttga agaacgatca
     9661 tagaggtaga gaacctcagt cggctgcata taagccttaa cccctgtcgg aaccaagaca
     9721 atctctcctg gcgcaacaac tgtacgcaca gcaaccttta agtcgtaacc agccgcatgc
     9781 gctgtctcac gcttgggcaa taaattttca tctgtaaaat tcgaaaccaa ttcaaaacca
     9841 cgaattttca taattttctc ttttctatta tcatttattc tagattattc tatcttattt
     9901 attcggaaaa agcacgaaaa aaagagcaca caattcaaat cgcttagggc tgctggattc
     9961 ctcccctgac ccgcttcacg cagaactgtt gctccactat ttattatatc acattcctat
    10021 tcattttaaa agcaaaatta ttttttccgt ctatttctaa aaaaatcctg catgatagct
    10081 gcgcattcat cttccaaaat tcctatttca acctccacac gatgattgag acgctcatct
    10141 gtcaagatat tgtacaaact cccagcagcg ccaaatttct ggtttttagc cccatagacc
    10201 acatttggaa tgcgggcaag cccaatcgcc ccactacaca tgacacaagg ttcaatggtc
    10261 acaaaaagtg tgcaatccag caagcgccag ctctcctcac tcaagttcgc atcctctata
    10321 gccataattt ccgcatgcat aaccgctcgc tgtaattcct cacgcgcatt atgcccacga
    10381 ccaatgattt ccccatcttt gacaatcaca caaccaattg gaatttcatc gtgttcaaga
    10441 gcaatctcag cctctctcaa agcctccctc ataaagactt ctttttcttc aagcgtataa
    10501 tacatctctt ttctcttttc ctacttatcg attttattat tatatcatga atcccaacac
    10561 aaaaaaagcc accgaatgcg gtgactctat agggagatta ttatgaaaaa gaaaagttta
    10621 ggatatttgt tacaacaagt taggaggtct tcttgtaact gtctatagta tacccgacct
    10681 atcttaaaca aatcttaaaa atctcttagg accaaacact ttctaaaata tttgtttgtt
    10741 cacgaccagg acctactgag aaagtagaaa tacgaacgcc aaccaattca ctcacacgac
    10801 gaacatagtt acgcgcattc tcaggaagat cttccaaatt gcgaactccg gtaatatctt
    10861 ctgaccaacc tggcaactct tcatagatag gcttgcaacg tttcaattgc tcaagactag
    10921 ctggatagta gtcaatacgt tgaccgtcaa gatcataggc cacacagatt ttcacagtat
    10981 ccaaaccgct caaaacatca atagagttca aagaaaggtt agtaatacca gaaacacgac
    11041 ggctatgacg catcacaact gagtcaaacc aacctacacg acgtggacga ccagttgttg
    11101 taccatattc atgacccact tcacggatac gttctcccac ttcatcaaac aactcagttg
    11161 ggaaaggacc atctcctaca cgactcgtat aagctttaca tacacctaca accttgtcaa
    11221 tcttgcttgg accgatacca gaaccaattg tcacaccacc agctacaggg tttgatgacg
    11281 taacaaatgg ataagtacct tggtcgatat ctagcataac accttgtgca ccttcaaaaa
    11341 gcacacgttt gccattatca agcgcatcat tcaagataac agatgtatct atcacgtatt
    11401 tcttgatttg ttgaccatat tcgtaatatt cttcaaaaat atcatcgaaa acaatcgctt
    11461 tactgtcata caatttttca aaaagacgat tcttttcagc aaggttacgt tctaaacgct
    11521 cacggaaaat atctttatct aaaagatctg caatacgaat tccaacacga gcagccttgt
    11581 ccatataagc tggaccaatt cccttaattg tcgtaccaat cttattgtcg cccttagctt
    11641 cttcttgcaa gcgatccaac tcgatatgat aaggcaaaat aacatgcgca cgatcagaaa
    11701 tacgcaagtt atcagttgtt acaccttcct catgaagata gctcaactct tttacaagag
    11761 atttaggatt tacaaccata ccattcccaa tgacagatat tttttcaggg aagaaaatcc
    11821 cagatggaat caagtgcaac ttaaatttct taccgtcaat cacaatcgtg tgaccagcat
    11881 tatcaccacc ttggtaacgt gcaattactt ctgcattcgc tgaaaggaag tctgtaatct
    11941 tccctttacc ttcatcaccc cattgggtac ctacaacaac aactgaagtc ataattttgt
    12001 ctgagccctc aggctcttcc tttctcacat acatggcagg actctcacct gcaattatat
    12061 cttacaattt attataataa aaaatcgcct ttttatcaag aagaaacaat agaaagattt
    12121 gctatttcca actattaaaa aatgatttag aaaaattact agctatttac tattatcttt
    12181 ccataaaaga gtaaattagt tcggaaattt actaaaatta cctcaacaag aaataaaacc
    12241 ccgattcatt accaattttt caagatacaa acgataagca acacgataat ggtaaacgat
    12301 aaaatcccta cgacaaccaa tgccatatct cactaaataa taaattaaaa atttaaaatg
    12361 aacatgttcc cagtcaaaat tatcaccaaa tgtaggacca tactcttctt caatactatc
    12421 atagaaatta gccatctgct cataaatttt ttgtaacata atcaaatact ccttttcttt
    12481 tttataaact tattctaaca aaaaatttta cacattcact atcaattcct gaattgttaa
    12541 aataccaggc tcaacaagat aaaaatagga aaagttgaca gaatagaaac aaatttgcta
    12601 cctaaatttc aaacgattaa agttctaaaa agaccgcctt aaaacttttg ggtaaatcct
    12661 attagaacta taatattttt atataggtat tatggttctg attttctagt ataaaaaatt
    12721 caaactagaa ttcttcttag aaaaggccgt tttaagtgct atcactgttc aaaaatgagg
    12781 gtcgctgaaa cttctatcgt caagaagaat catcaaattc cttgtatcat caaccaaaag
    12841 attgctcaga agctaattaa aaagacttct atgaccgaca ttgatcatca gttgtctatt
    12901 tcaacttcaa ctgtcattcg caagatcaat gattttcact ttgagcatga tttttcgcgt
    12961 cttcctgaga ttatgtccta ggacgttgaa acagtccggg gagtgactgt ttcaatcggg
    13021 agatggagat gagctttatt gctcaagact ttgataagct caatatcata actgttcttg
    13081 agagcagaac acaagccatc atccgaaatc ccatgaatac aaggctatca agcgatactg
    13141 aaagctcatt caacaagata gtcagaaact gagtgataaa cgattttatc gccctacttt
    13201 tcgtatgcac ttgactaata aagaaattct agataaactt ttgagctatt ccgaagactt
    13261 gaaacaccac tataatctct atcagctctt gcttttccac ctctttttca gactctgttt
    13321 aaaaccttcc tcaaagataa agagaaaatc gtcaacgccc ttcaattacc ttattccaac
    13381 gccaaactgg aagccaccaa taatctcatc aaacttatca agtacaatgc ctttggtttt
    13441 aggaactttg aaaacttcaa aaaagaaagg acgaaatttg tcctttctag atcttcgctt
    13501 tcttcaaccc actacagttg acaaagaacc gaaaaatgtc tcgcctctaa tatggtcaat
    13561 tgaaacaaga acaagacaaa agagcctcat aaaaggtatt gcaacttggt aatacctttt
    13621 tggggt
//
LOCUS       DAVKJD010000047        13224 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKJD010000047 DAVKJD010000000
VERSION     DAVKJD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13224
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.047"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            43..1302
                     /locus_tag="VW157_001402"
                     /pseudo
     CDS             43..1302
                     /locus_tag="VW157_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            1398..2672
                     /locus_tag="VW157_001403"
     CDS             1398..2672
                     /locus_tag="VW157_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9552689.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKEHDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRQEKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYIVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            2682..3560
                     /gene="truB"
                     /locus_tag="VW157_001404"
     CDS             2682..3560
                     /gene="truB"
                     /locus_tag="VW157_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9552690.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVESGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            3801..4103
                     /locus_tag="VW157_001405"
     CDS             3801..4103
                     /locus_tag="VW157_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9552691.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            4211..4849
                     /gene="udk"
                     /locus_tag="VW157_001406"
     CDS             4211..4849
                     /gene="udk"
                     /locus_tag="VW157_001406"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9552692.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            4977..6317
                     /locus_tag="VW157_001407"
     CDS             4977..6317
                     /locus_tag="VW157_001407"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9552693.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQVTIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLIEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQWDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     RQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAETKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKNVKTKEI"
     gene            6295..6507
                     /locus_tag="VW157_001408"
     CDS             6295..6507
                     /locus_tag="VW157_001408"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9552694.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            6504..7379
                     /locus_tag="VW157_001409"
     CDS             6504..7379
                     /locus_tag="VW157_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9552695.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            7372..8187
                     /locus_tag="VW157_001410"
     CDS             7372..8187
                     /locus_tag="VW157_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9552696.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVFNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            8180..8611
                     /locus_tag="VW157_001411"
     CDS             8180..8611
                     /locus_tag="VW157_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9552697.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAENNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            8618..10285
                     /gene="recN"
                     /locus_tag="VW157_001412"
     CDS             8618..10285
                     /gene="recN"
                     /locus_tag="VW157_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9552698.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLYTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            10287..11015
                     /locus_tag="VW157_001413"
     CDS             10287..11015
                     /locus_tag="VW157_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9552699.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            11094..12917
                     /gene="lepA"
                     /locus_tag="VW157_001414"
     CDS             11094..12917
                     /gene="lepA"
                     /locus_tag="VW157_001414"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9552700.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(12998..>13224)
                     /locus_tag="VW157_001415"
     CDS             complement(12998..>13224)
                     /locus_tag="VW157_001415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552701.1"
                     /translation="TRKLAAGYSKHSFEVVDETDEVSSKHGFEIVDETDEVSSKHGFE
                     IVDETDEVSNHTYGKATLTWFEEIFEEYKN"
BASE COUNT         3923 a         2438 c         3165 g         3698 t
ORIGIN      
        1 ggtgctacaa tatccttaat aaaataatat ggaggtcacc ttatgactcg tgattttaaa
       61 tttgaaactc tacaactaca tgctgttcaa gttgtggctc cagctactaa gtctcgtgca
      121 gtgccgattt atcaaacaac attttttgtt tttgatgaca cgtaggaagg tgccgatatg
      181 tttgccttga ggaaaccagg gaacatttat actcgtatca ccaatcctac aacagctgcc
      241 cttgaaggtg gtgttgaagc gctagcaaca gcatcaggta tgactgcagt gacttatact
      301 attttggcga ttgcccatgc tggtgaccat gtagtggctg cttcgactat ttacggtgga
      361 accttcaatc ttttgaaaga accccttcct cgttatggta tcacaacaac ctttgtcgat
      421 attgataatt tggaggaagt agaagcagct atcaaagaca ataccaagct tgtcttgatt
      481 gaaaccttgg gtaacccctt gattaatatt ccagacctgg aaaaactggc agagattgct
      541 cataaacatc aaatcccact tgtgtcagac aatacttttg caacacctta tttgattaac
      601 gtcttctctc atggcgttga cattgccatt cactctgcga ctaagtttat cggtgggcat
      661 ggtacaacta ttggaggaat aattgtcgat agtggtcgtt ttgactggac ggcttcaggg
      721 aaattccctc aatttgttga cgagggtcca agctgccaca atttgagtta tactcgtgat
      781 gtgggtgcag cagcctttat tatagctatt cgagttcaat tgcttcgtga tacaggtgca
      841 gccttgtcac cattcaatgc tttcctcttg ctacaaagac ttgaaaccct ttcacttcgt
      901 gtggaacgcc atgtacaaaa tgctgagaca attgttgatt ttcttgtcaa ccatcctaag
      961 gtagaaaagg taaattatcc aaaacttgca gatagtcctt atcatgcctt ggctgagaaa
     1021 tacttaccaa aaggtgtggg ctcaatcttt accttccatg tcaaaggtgg ggaggccgaa
     1081 gcccgcaagg tcattgataa tttggaaatc ttctcagacc ttgctaacgt ggcagatgcc
     1141 aagtctcttg ttgttcatcc agcaacaacc actcacggtc aattgtcaga caaagaccta
     1201 gaagccgcag gtgttacacc aaaccaaatc cgcttgtcga tcggacttga aaatgtagag
     1261 gatttgattg aagatttgcg cttggccttg gaaaaaattt aaagtaaaag aagataaaca
     1321 gtgggttttg actcactgtt tttgattttc cctcaggcat gatataatgg ttaaagaagt
     1381 ctagaaagag gaacgatatg aacgaaatca aatgtcccaa ctgtggggaa gtctttacag
     1441 taaatgaaag ccagtatgct gaacttttat cccaagtgag aacggcagag tttgataagg
     1501 aactacatga tcggatgagg caggaactgg ccttggctga gcaaaaggct atgaatgagc
     1561 aacagactaa actggctcag aaggatcaag aaattgcgca attgcagagt cagatccaaa
     1621 actttgatac agaaaaagaa ttggccaaga aagaagttga acagacaagc catcaggctc
     1681 tcttggctaa ggacaaggaa gtacaggctt tggagaacca attggccact ttgcgtttgg
     1741 agcatgaaaa tcaactgcaa aagacccttt ctgacctaga aaaagaacat gatcaggtca
     1801 aaaatcagct cctcttgcaa gaaaaggaaa atgagttgtc tttggcttct gttaagcaaa
     1861 actatgaagc ccagcttaaa gcagctagtg aacaagtcga gttttataag aattttaagg
     1921 ctcaacaatc tacaaaagcg attggggaaa gcctagaaca gtatgcagag agtgagttta
     1981 acaaggttcg tagtttcgcc tttccaaatg cttactttga gaaggataac aaggtctctt
     2041 cgcgtgggtc taaaggggac tttatcttcc gtgagtgtga tgaaaatgga gttgaaatca
     2101 tttctatcat gtttgagatg aaaaacgaag cggacggaac agagaagaag cacaaaaatg
     2161 cagattttta caaggaattg gacaaggacc gtcaggagaa gaactgtgag tatgccgttt
     2221 tggtgaccat gcttgaggcc gataatgact actttaacac agggattgtt gacgtcagcc
     2281 acgagtatga aaaaatgtat attgttcgtc ctcaattctt tatccaattg attggtctct
     2341 tacgtaatgc ggcgctaaat tccctaaaat acaagcagga gttggccttg gttcgcgagc
     2401 aaaatattga cattacgcat tttgaggaag acttggatgc ctttaagcta gcttttgcta
     2461 agaactataa ttcagcttcg actaactttg gaaaagctat tgatgaaatc gacaaggcca
     2521 tcaaacgcat ggaagaggtt aagaaattcc tgaccacatc tgaaaaccaa ctccgtttag
     2581 ctaacaacaa attggaagat gtctctgtta aaaaattgac ccggaaaaat ccaacaatga
     2641 aagcgaagtt cgaagcactg aagggggagt agaaagcaaa aatgaacggt attatcaact
     2701 taaaaaaaga agcaggaatg acctcgcatg atgcggtttt taaactgcgt aagattttgg
     2761 gaaccaagaa aattggtcat ggtggaacct tggatccgga tgtggtgggt gttttgccga
     2821 ttgcggttgg caaggcgaca cgcatggtcg agtttatgca ggacgagggt aagatctatg
     2881 agggggaaat tactctgggt tattccacga cgactgagga tgctagtggg gaagtgatcg
     2941 cagaaacccc tgttttgtct cccttggatg aaaagcttgt tgatgaagcg attgctagct
     3001 tgactgggcc tattacccag attcccccta tgtattcggc agttaaggtt aatggtcgca
     3061 agctctatga gtatgcgcgt gctggtcagg aagtggagcg tccagaacgt caggtgacca
     3121 tttatcaatt tgagcgaaca agtccgattt cttatgatgg ccaacttgcc cgattcactt
     3181 ttcgtgtaaa atgcagtaaa gggacttaca tccgtacttt gtcagttgat ttgggtgaaa
     3241 agcttggtta tgcggctcat atgtcccatt tgactcgtac tagtgctgct ggcttacaat
     3301 tagaagacgc tcttaccttg gaggaaattg ctgaaaaagt agagtctggg caattagatt
     3361 ttctccatcc tttagagatt gggacaggtg accttgtcaa agttttccta actccagaag
     3421 aggctacaga agttcgcttt ggtcgtttta ttgagctaga ccaaacggac aaagaactgg
     3481 ctgcctttga agatgataaa ttgttagcca ttctagaaaa acggggcaat ctctataagc
     3541 caaggaaggt ttttagctag atcgtttagg aataaaaatc gggtgataga taacaattgc
     3601 ttgataaaac cccatactaa tagtagaatg gttttgggaa ttataatatt ccaattgttg
     3661 cgagttgtag atactcaaat aatctatata gaaatttaga ggtgtgaaat gaagcaactt
     3721 aaaattcttt cagataaata tttagagtcc attacaggtt ctggtgggaa cttaggccca
     3781 ggatttggtg tgataattcc atgatgcgaa atgagtttcg agaaagggtg gagcaacttc
     3841 ttcaacaaaa agaaataaat gaaaatagtg agttgagtca cctgtttcgt cttgctatac
     3901 aaaatttaga cagaaatgaa aaataccaat cggtcatggc caatttgagt caagggttgt
     3961 cactttacct catgacgcat cattaccagg cacctaagtc tgtcattgat tttggtttat
     4021 ggattgccaa agctcctagc caggaaagag ggagactggc tttcttgcag atgcttgctc
     4081 aaaccctaca gggctttcgt taaaactgtc gaacatcaga gggaagactc tggtgttttt
     4141 tgctgatgag actataaatt ttgtaaaaaa tgtgatagaa tagacgacgg ataaaataac
     4201 ggaggatagc atgcaaaata gaccaatcat tattggagtg acaggtggtt ctggtggtgg
     4261 taaaaccagt gtttcaagag ccattttatc gcattttcct gatgaaaaga tttccatgat
     4321 tgagcatgat tcatactaca aggatcagtc tcatttgacc tttgaagagc gtgtcaaaac
     4381 caactatgac catccttttg cctttgatac agacttgatg atcgagcaga ttaaggaatt
     4441 gttggcaggg cgtccggtgg acatcccgac ctacgactat acagagcata cacggagtag
     4501 caagacctat cgtcaggagc ctcaagatgt ctttatcgtt gagggcattt tggtcttgga
     4561 ggacaagcgt ctgcgcgatt tgatggatat caagattttt gtggatacgg atgacgatgt
     4621 gcgtattatt cgtcgtatca agcgtgatat ggaggagcgt ggtcgtagcc ttgatagcgt
     4681 tattaaccag tacttaggtg tggtcaaacc aatgtaccac cagtttatcg agtcaactaa
     4741 gcgttatgct gatatcgtca ttcctgaagg ggttagcaat accgtggcta tcgacctgtt
     4801 gacgaccaag attgcaaaga ttttggaaga agctcgaaac agcaaataat cacatgtgga
     4861 ggccttacct cctttttcta tttttccctc ataatggtac ataaatggag atttttaggc
     4921 atatttttgg tataataata cccatgaaag agcaagtaaa tgaatagtag gtggagatgg
     4981 aaaagtattt atcggtaaca actttgacca agtatctgaa aatgaaattc gataaagacc
     5041 catacttgga acgggtctat ttaactggtc aagtttccaa ctttcgtaaa cgacctactc
     5101 accaatattt ctccctaaag gatgaccatg cagttattca agtgaccatc tggtctggga
     5161 tttatcagaa attagggttt gacctggaag aaggaatgaa gatcaatgtg attgggcgtg
     5221 tacaggtcta tgaaccaagt ggtagctact ccatcatcat tgaaaaagct gagcctgatg
     5281 gggttggggc gcttgcgatt cagtttgaac aacttaagaa aaaattgata gaagaaggcc
     5341 tgtttcaaga acgcttcaag caagctctac cccaattttc taagagaatt ggggtagtaa
     5401 ccagccgtag tggagccgtt attcgagata ttatcacgac cgtcagcagg cgattcccag
     5461 gtgttgacat ccttctttat ccgaccaagg ttcaaggtga aggggctgcg gaggaaattg
     5521 ctagaaatat tgcgcgtgct aatcaatggg acgatttgga tttgctcatt attggtcgtg
     5581 gaggtggttc tattgaggat ctctgggcct ttaacgaaga aattgtggta cgagctattt
     5641 ttgaatctcg tttgccagtt atctctagtg tggggcatga gacggatgtg accttggcag
     5701 attttgtggc agatcgacgc gctgcaacgc caacagcggc ggctgaactg gcaacacctg
     5761 tgaccaagtt ggatgtatta gctcatttgc aaaatcagga aaaacggatg gcaacggcag
     5821 tccgaaatgt tctatctaag aaacaagagg ctttgaaaaa atgcagtcag tctgttatct
     5881 ttagacaacc tgagcgcttg tatgacggtt atttgcaacg cttggaccaa ctgcaactgc
     5941 gtttgaaaca aagtttgcga actcggattt ctgataacag acaattagtt caagcaagaa
     6001 ctcatcaatt agtacaatta tcacctgtta ccaaaatcca acgctatcaa gaccgtttag
     6061 gacagttgga caagctctta ggtagccaaa tggcgttagt ttatgacgcc aaggttgctg
     6121 agaccaagcg actttcggaa gctttgctca tgttggatac tagccgaatc gtggcgcgtg
     6181 gttatgctat tgtaaaaaaa gaagagtccg ttgtagattc ggttgagagt ttgaagaaaa
     6241 aagaccaagt aacgcttttg atgcgagatg gtcaagtaga attagaggtt aaaaatgtca
     6301 aaacaaaaga aatttgagga aaatctagca gaactggaaa ccattgtcca aagtttggaa
     6361 aatggtgaaa ttgctctgga agatgcgatt actgcctttc aaaagggcat ggtcttgtca
     6421 aaagagctcc aagctacgct ggacaaggct gaaaagacct tggtcaaggt catgcaagaa
     6481 gacggaacag aaagtgattt tgaatgaaaa agcaagaaaa attagctctt gttgagtcgg
     6541 ctttggaaga tttttatgga gaccagcagt ttgcctctag tttacgggag tctgttctct
     6601 attctattca tgctggtggc aagcgtattc ggccttttct cttgttagaa gttctggaag
     6661 ccttgcaggt tgccatcaaa cctgctcacg cgcaggtagc tactgccttg gagatgattc
     6721 atacagggag cttgattcac gatgaccttc ctgctatgga tgatgacgat tatcgaagag
     6781 ggcggttaac caatcacaag aaattcggtg aagctatggc cattttggct ggagatgcct
     6841 tattcttaga cccatatgcc ttgattgcgc aggcagattt gccaagtcag atcaaggtgg
     6901 acttgattgc caacttatcc cttgcttcag gtagtctggg tatggtggca gggcaagttt
     6961 tggatatgga gggcgaacac cagcacttgt ctctggaaga acttcagact attcatgcca
     7021 ataagactgg gaagttacta gcctatccct tccaagcggc agctattata gctgaattgt
     7081 cacctgaaat gcaggtgaag ctgaaaactg tgggtgaatt gattggactt gcttttcaag
     7141 tcagagatga tgtactggat gtgacagcta gttttgagga aatcggcaag acacctcaaa
     7201 aggatctgca ggcagaaaaa tcaacctatc ctgccttgtt gggcttggaa gagtccattg
     7261 ccttttgtaa ccaaaccctg gatcaagcta atgaaaaatt agaagaaatt gcccagcagc
     7321 ttccctttga aacagaatcg attgtaagtg tagtagaaag tttgagaatc aatggctaag
     7381 gaaagagtgg atgtactagc ttataaacag gggttgtttg aaacgagaga gcaggccaag
     7441 cgaggtgtga tggctggcct agtcgtagca gtctttaatg gagaacggtt tgacaagcca
     7501 ggagagaaaa ttccagatga caccgaatta aaactcaagg gggagaaact caagtatgtc
     7561 agccgtggtg gtttgaaact ggaaaaggcc ttgcaggtct ttgatttgtc ggtggatggc
     7621 gcgactacga ttgatatcgg ggcctctact ggaggtttta ccgatgtcat gctacagaat
     7681 agtgccaagt tggtctttgc agtcgatgtt ggtaccaatc agttggcttg gaaattacgc
     7741 caagacccac gagttgtcag catggagcag ttcaattttc gctatgctga aaagactgat
     7801 ttcgagcagg agccgagctt tgccagtatt gatgtgagtt tcatttccct tagtctgatt
     7861 ttgccagcct tgcaccgtgt cttggctgat caaggtcagg tggtagcact tgtcaaacct
     7921 cagtttgagg caggacgtga gcagattggg aaaaatggaa ttattcgaga tgctaagatt
     7981 catcagaatg tccttgaatc tgtaacagct atggcagtag aggcaggttt ttcagtcctt
     8041 ggcttggact tttctcccat ccaaggtgga catggaaata ttgaattttt agcgtatttg
     8101 aaaaaagaaa agtcagcaag caatcagatt cttgctgaga ttaaagaagc agtagagagg
     8161 gcgcatagtc aatttaaaaa tgaataaaaa agaaagactt gaaaaaatta gaagattggt
     8221 gacagattat caaatcggca cgcaagaaga aattgtagaa catttgaaag aagcaggtat
     8281 cactgccact caggcgacgg tatcccgaga tatcaaagag ttaggtattg tcaaaattcc
     8341 tttgagagac aacacctatg tctacgaatt accaaaatca atcgtaaaaa gtctgcaact
     8401 ggctgaaaac aatatcgaat cggctgaatt gatggataag atgatcaatc tccaagttat
     8461 tccaggaaat acggcttttg taaaagctca gttaatcgat acttttgcag acaagatttt
     8521 tagttgtttg actgatgata gctcgatttt agtcattgcc agaagtggaa gtctagcaga
     8581 ggaaatcttt gaacaagtaa aaaattggta ggtctatatg ttacttgaaa tttcgataaa
     8641 aaactttgcc attattgagg ctatttccct caattttgaa aaggggatga ctgtcctgac
     8701 tggtgaaacg ggtgcaggga agtcgattat cattgatgcc atgaatatga tgttgggagc
     8761 tcgtgcgacg acagatgtta ttcgtcatgg tgcaccaaag gcagagattg aggggctttt
     8821 ctcagttgag aatagtcgcc ttttacagga aatttttgat gagcaaggtt tagaattagg
     8881 tgatgaaatt atcatccgtc gggaaatctt gcaaaatggt cgtagtatca gccgtgtaaa
     8941 tggtcagatg gttaatctgt ctgttttgcg agctattggg caacatcttg tagatattca
     9001 tggtcagcat gaccatgagg agttaatgcg tccccaactg catatccaga tgttggatga
     9061 atttggtgat gccgcttttt gggacttgaa agaaacctat caaacgagtt ttgatgccta
     9121 tcggaaaatg cgcaagcagg ttctggaagt caagaaaaac caacaggaac acaaggcacg
     9181 tatcgaaatg ttggaatttc aaatggcaga gattgaggca gcaaacttgc aggctggaga
     9241 agacttggct ctcaatcaag agcgagataa actcctcaac cacaaaaata ttgcggatac
     9301 actgaccaat gcctacagta tgttggacaa tgaagatttt tcaagtctgg ccaatgttcg
     9361 ttcagctatg aatgacatgg aaagtgtcga agagtatgat cctgaatacc gtgaaatttc
     9421 aagctctctg tctgagacct actatgtttt agaagacatt agcaaacgtt tggaagctat
     9481 cattgaggac cttgattttg atggcaatcg cctgatgcag gttgagaatc gtttggacct
     9541 cctttatact attactcgta agtatggtgg gactgttgat gatgttttgc tttattttgc
     9601 caagattacg gaagaataca atctcttgac aggcaataat ctttcgtctg aggacatgga
     9661 agcagagctt aagaagttgg aagtcaatct tgtcaatttg gcaggtcaac ttgcttctgc
     9721 tcgtcataat ttggctcagc aactcgaagc tgagattaaa caagaactgc aagatcttta
     9781 tatggaaaaa gcccagtttc aggttcgttt tagtaaggga aaattcagtc gtgagggaaa
     9841 tgaaatggtt gagttttata tttcaaccaa ccctggagaa gactttaaac ccttggttaa
     9901 ggttgcttct ggaggggaat tatctcgtct catgttagcc attaagtctg ccttttcacg
     9961 taaagaaggc aagactagca ttgtctttga tgaggtggat acgggagttt caggtcgtgt
    10021 tgctcaagct attgctcaga aaattcataa aattggtcag catggtcagg ttttggctat
    10081 ctcccatttg ccacaagtaa ttgcgattgc agattatcaa ttctttattg agaagattag
    10141 taatgaccat tcaacggttt cgactgttcg tctcttgacg gtcgaagagc gagtggagga
    10201 agttgccaag atgttggcag gtgatgatgt gacagaagca gccctgacgc aagcaagaga
    10261 attgttgaga aacagggaga aataagatga cagattatta tgtaattgga gatgttcacg
    10321 gaaaagctgg gatgctggaa gaccttctca aaacctggga tggtcagccc cagttgctct
    10381 ttctaggtga tttgattgac agaggtgagg atagtcaccg tgttcttgaa atggtcaagg
    10441 acttggtgga caatcaaggg gctatctgtt tgtccggaaa ccacgagtat atgtttttga
    10501 cttggctaga tgacccagaa gaaagttatg accattatag tcgcaatggt ggagatacaa
    10561 ccattaactc tatcctaggt cgtcccttgg atgcaccagt tgatggagta gaagatgcca
    10621 agcgggttgc cgctgaagcg gcagacttgg tcgaattcat tcgtcaaatg ccatttgtag
    10681 tagagacaga caagtatatc tttgttcacg caggtattga tttgaccttg gacgactggc
    10741 atgaaacaac agattataaa aaagtatggc ttagaaaacc attccacgga gctgaaaatc
    10801 atactggaaa aaccattgtc ttcggtcata caccggtcta tggtttgtta cagcaagaac
    10861 gcggtacatc tgagctttgg actacagaag atggcaagat tggaatggat ggaggagctg
    10921 tttatggtgg tgtccttcac gggattgtct ttacagacca aggaatgaca gaacaccact
    10981 ttatcgaaaa tgacggcttt gtcgctgaag attagtactc ctagcagggt atggtcttgt
    11041 caaaatatca aaaacaattt ataataaata gataccctga aaggaagaga atcatgaact
    11101 tagaagaatt gaaaaaacga caggagaaga tccgtaactt ctctattatc gcccatattg
    11161 accacggaaa atcaactcta gcagaccgca ttttggaaaa aacagagacg gtttcaagtc
    11221 gtgaaatgca ggcccagctt ttggatagca tggagctaga gcgggaacgt ggaattacta
    11281 ttaagttgaa tgccatcgag ttgaattaca ctgcaaaaga tggggaaact tatattttcc
    11341 acttgattga cacaccaggg cacgttgact ttacctatga agtttcacgt tcgctagctg
    11401 cctgtgaggg tgctattttg gtggtcgatg cggctcaagg aattgaggct caaactcttg
    11461 ccaacgttta tctggctttg gataatgatt tggaaatcat gcctatcatt aataaaattg
    11521 acctgccggc tgcagatccg gagcgcgtgc gtacagagat tgaagatgtg attggtttgg
    11581 atgccagtga agcagttttg gcttctgcca aggctggtat tgggattgaa gaaatcctcg
    11641 agcaaattgt agaaaaagta ccagcaccaa cgggtgatgt gacggcgcca cttaaggcat
    11701 tgattttcga ctctgtttac gatgcttacc gtggggttat cctccaagta cgtgtcatgg
    11761 acggagtggt caaacctggt gataagattc agctcatgag caatagtaag acctttgatg
    11821 tggccgaagt cggtattttt acaccaaaag cggttggtcg tgatttcctt gcgactggtg
    11881 atgttggtta cattgcggcg tctatcaaga cggttcagga tactcgtgtg ggtgataccg
    11941 ttaccttggc aaccaatcct gcggcagaac cattacatgg ttataagcag atgaatccta
    12001 tggtctttgc gggtctctac ccaatcgaat caaacaagta caatgacctg cgtgaagcgc
    12061 ttgaaaaatt gcaactgaat gatgctagtc ttcagtttga accagaaaca tctcaggcac
    12121 ttggatttgg ttttcgttgt ggatttcttg gacttctcca tatggatgtt atccaggaac
    12181 gtttagagcg tgagttcaat attgacctca tcatgacagc tccgtctgtt atttacaaag
    12241 ttaatttgac cgacggtgag tctatggatg tgtctaaccc atctgagttt ccagacccaa
    12301 ctaagattgc gaccattgaa gagccttatg tcaaggcgca aatcatggta ccacaggagt
    12361 tcgtcggagc agtaatggag ctagctcagc gtaagcgtgg ggactttgtg actatggact
    12421 atattgatga taaccgtgtc aatgttatct atcaaattcc tcttgctgaa attgtctttg
    12481 acttctttga taaacttaag tcttcgacac gtggttatgc aagctttgac tacgaattgt
    12541 cagaatatcg cccatctaag ctggtgaaaa tggatatcct tctcaatgga gataaggtgg
    12601 atgccctcag ctttatcgtt cacaaggact ttgcctacga acgtgggaaa ctcatcgttg
    12661 ataaactcaa gaaaatcatc cctcgccaac aatttgaagt tccaatccaa gcggctattg
    12721 gacacaagat tgtcgctcgt actgatatca aggcccttcg taagaacgta cttgctaaat
    12781 gttatggtgg tgacgtttct cgtaagcgta aactccttga aaaacaaaaa gctggtaaga
    12841 aacgcatgaa atccatcgga tcagttgaag ttccgcaaga agccttcctc agcgtcttga
    12901 gtatggatga agaataagtt atcagaaatg cctttccttg ttcacaggaa atttatataa
    12961 gaatcaatag taaagcaggc ttagatggtc tgctttttta gtttttatac tcttcgaaaa
    13021 tctcttcaaa ccacgtcagc gtcgccttac cgtatgtatg gttactgact tcgtcagttt
    13081 catctacaat ctcaaaacca tgttttgagc tgacttcgtc agtttcatct acaatctcaa
    13141 aaccatgttt tgagctgact tcgtcagttt catctacaac ctcaaaactg tgttttgagt
    13201 aacctgcggc tagcttccta gttt
//
LOCUS       DAVKJD010000048        13176 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKJD010000048 DAVKJD010000000
VERSION     DAVKJD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.048"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            1..870
                     /locus_tag="VW157_001416"
                     /pseudo
     CDS             1..870
                     /locus_tag="VW157_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(987..1334)
                     /locus_tag="VW157_001417"
     CDS             complement(987..1334)
                     /locus_tag="VW157_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9552702.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEIEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(1445..3220)
                     /gene="spxB"
                     /locus_tag="VW157_001418"
     CDS             complement(1445..3220)
                     /gene="spxB"
                     /locus_tag="VW157_001418"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9552703.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(3427..5670)
                     /locus_tag="VW157_001419"
     CDS             complement(3427..5670)
                     /locus_tag="VW157_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9552704.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEILSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(5680..6051)
                     /locus_tag="VW157_001420"
     CDS             complement(5680..6051)
                     /locus_tag="VW157_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9552705.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(6062..6457)
                     /locus_tag="VW157_001421"
     CDS             complement(6062..6457)
                     /locus_tag="VW157_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9552706.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      6641..6739
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6823..7614
                     /gene="thiD"
                     /locus_tag="VW157_001422"
     CDS             6823..7614
                     /gene="thiD"
                     /locus_tag="VW157_001422"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9552707.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7824..8456)
                     /locus_tag="VW157_001423"
     CDS             complement(7824..8456)
                     /locus_tag="VW157_001423"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9552708.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(8449..9252)
                     /locus_tag="VW157_001424"
     CDS             complement(8449..9252)
                     /locus_tag="VW157_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9552709.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTSPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(9252..9776)
                     /gene="thiW"
                     /locus_tag="VW157_001425"
     CDS             complement(9252..9776)
                     /gene="thiW"
                     /locus_tag="VW157_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9552710.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERIE"
     gene            complement(9781..10473)
                     /gene="tenA"
                     /locus_tag="VW157_001426"
     CDS             complement(9781..10473)
                     /gene="tenA"
                     /locus_tag="VW157_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9552711.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(10484..11134)
                     /locus_tag="VW157_001427"
     CDS             complement(10484..11134)
                     /locus_tag="VW157_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9552712.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYISKKTKLIYLLFFLLLQTSLFL"
     gene            complement(11136..12521)
                     /locus_tag="VW157_001428"
     CDS             complement(11136..12521)
                     /locus_tag="VW157_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552713.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILLQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRSLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(12522..13082)
                     /locus_tag="VW157_001429"
     CDS             complement(12522..13082)
                     /locus_tag="VW157_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9552714.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
BASE COUNT         3958 a         2906 c         2562 g         3750 t
ORIGIN      
        1 atgaggagtt cggactcgac tctctccttc aagaaacacg tggtggtcgt aaccatgcat
       61 atatgacagt tgaggaagag aaagcctttc ttgcccgtca tttgaaggct acagaggcag
      121 gagaatttgt tacaattgat gccttatttc aggcttataa aaaggagtta ggtcgttcct
      181 acacacgtga tgccttctat caactgttga agcgccatgg ttggcgaaat attacgccac
      241 gtccagaaca tcctaagaaa gcagacgctc aaaccattgt cgcgtctaaa aataaaatct
      301 caattcaaga agaaaagaaa gcgatttaaa accagtagac gttttcgtaa ggttcgcttg
      361 atgtaccaag atgaggctgg tttcggtaga atcagtaaac tgagatcttg ttggtctcca
      421 ataggagtag gtccacatat ccatagtcac tatatacgag aatttcgcta ttgttatgga
      481 gctgttgatg cctatacagg cgaatcattt ttcttaagag ctggtggatg taatactgag
      541 tggatgaacg tctttttaga agagctttca caagcttatc catatgatta ttttttactc
      601 gttatggaca atgctatatg gcataaatca agtaccttaa agattccgac taatattggc
      661 tttgcattta ttcctccata cacaccagag atgaacccca ttgaacaagt gtggaaagag
      721 attcgtaaac gtggatttaa gaataaagcc tttcgaactt tggaagatgt catgaatcaa
      781 ctccaagatg tcatacaagg attggagaag gaggtgataa agtccatcgt taatcggaga
      841 tggactagaa tgctttttga aagcagatga gtattatatg caatttcttt atataaaaag
      901 accggattgc tccgatcttt caatagttca tattctcaat ttctatttta aaaatagcta
      961 aggttaacgt caaatgacta cgcgacctat ttcatacgat aaaaatcaag cactagacca
     1021 gcaggtcctt gaactaataa ggactctgtt ccccaatcgg ttacagttgg tccgtgtaaa
     1081 acctttatac caagctcgtt caaccgtttg tagttctggt ctacatcctc aatctcgata
     1141 tgaataatga ttcctgactg aaagttttcc aaaggaacca aatgattttg tgacaacata
     1201 aggcagtgac taccaatcgt aaactgagca aaaccatcat cagcataatc tgccttttta
     1261 tccaagatat gctccaagtc agcacagact tggggaacat ttgaaacgat aatatctaat
     1321 tgatttaaat tcatttactc tcctccataa aaagaccgga ttgctccgat cttttaaagt
     1381 tctgctctat gaaaatcaaa gaataaagtc tacaagtttc atatttgatt ttcggcgaga
     1441 ggaattattt aattgcgcgt gattgcaatc cttcttcttc caagaagaga cggaatggta
     1501 cgagttcttc tgcttcgtat ttttccttga aggctttgat agcttcttct gagtgaagtt
     1561 ttggatccaa ttcaagtact tctactggaa gtggacggtg ttgagtgatg cgagcatcga
     1621 tgacaacagt tttaccttct ttgttcaatt taacagcttc tgcaacaact gcatcgatgt
     1681 cttcgatacg gtcaactgtg aatccaacag ctccttgagc ttccgcaatt ttagcgtagt
     1741 cagcgtttgt gaagtctaca ccaaacaagt gtttgtttgt atcttcgtat ttgttcttga
     1801 tgaagccgta ctcagcattt gagaagacaa ggttgataac tggaaggtcg tattgaacgt
     1861 ttgtgataac gtctgggtag cacatgttga atgctccgtc acccatgatg ttccatactt
     1921 ggcgatctgg attgtctttc ttagcagcga taccaccagg aagggcaata cccattgtcg
     1981 caaagagcgg agatgtacgc cacatgttct taggtgtcat gtgaaggtga cgagtagatg
     2041 tttgagtagt gttacctacg tcgattgagt agatagcgtc ttgatcagca tgtttgttga
     2101 ttgcattgta aacttgatac aattgcaatt caccctcagt tttaccttcg agtttgttca
     2161 tgtaatcacg ccagttttgg ttgttcttaa cgtttgcacg ccaccatgga gttgattcaa
     2221 ctgggtttac tttgtcaagg atagctttag ctgcttgacc agcatcacca aggattgaag
     2281 cgtcaagggc atgacgttta ccaagtttgt aagggtcgat atcgacttgg atgaattttt
     2341 cagtgttctt gaatgcttcg taaacttcag caaatgggaa gtttgaacca aggaaaagaa
     2401 ctgtgtctgc ttcaaagacc acttcgttgg ctggtttcca accaacacgg taagcagaac
     2461 ctgtcaaacc ttcatagttc cattcgaaag cttcaaagtt tttaccagtt gtgatgattg
     2521 gtgctttgat tttacgtgac aattcagtaa tcacttcacc agctttaaca ccaccaaatc
     2581 cagcatagat aactgggcgt tcagcattgt tcaagatttc aacagctttg tcgatttcaa
     2641 cttcgttcaa agcaggagcg atgaatgagc gttcgtatga acctgaaccg tagtatgagt
     2701 tttcgtcgat ttcttggaaa ccgaagttta ctggaatttc aacaacagct ggaccttttt
     2761 tagaaattgc agcacggcag gcttcgtcaa ttacttttgg caattgctca gcgtaagcta
     2821 cacgtttgtt gtaaacagcg ataccgttgt acattgggtt ttggttaagc tcttggaaag
     2881 catccatgtt caattcgtta actggacgtg atccaaggat cgctaggaat ggagtgttat
     2941 ccatagctgc atcgtaaaca ccgttaatca agtgagtcgc acctggacca cctgaaccaa
     3001 ctgcaacccc gattgagccg ccgaatttag cttgcataac cgctgcaaga gcacctgtct
     3061 cttcgtggcg aacttgtaag aagcggatat ctttgtcttc agccaaagcg tccatcaatg
     3121 agctgagtgt tcctgatggg ataccgtaga ttgtatctac gccccatgtt ttcaatacgt
     3181 taagcattgc tgcagatgca gtaattttcc cttgagtcat aatgataact ctccttcaat
     3241 ttttttagat ttggagaata cgattacata gaattggaaa cgttctccaa atttttacta
     3301 ttccactgta tcatatttat gctgactttt ctaaaaatct gctcaaaact ctctattctc
     3361 tattctaata cagttttgaa agttctgtca tttctgtttt ataacaaaga aatctagtca
     3421 ttacttttag tctattttac taaaatttaa cagaagggaa ctggtcagaa cagatacaga
     3481 actaaaggcc atggctagac ctgccaattc tgggttgaga gccagtccaa cacctgaaaa
     3541 gactcctgct gcaatcggaa ttccgacaac attgtagata aaagcccaga aaagattgag
     3601 tagaattcga tgaaaggttt tcttactcat atcaaaggca cgaaccactc ctaaaagatt
     3661 attggttgtc aacaccaaat ctgctgactc gatggcgata tctgttccag ctcccatagc
     3721 aatccccaca tctgctacac taagggcagg agcgtcattg ataccgtccc caacaaaggc
     3781 tactttccct gactgttgca gtttatggat ttcatgggct ttttcttctg gcaagacgcc
     3841 tgcaatgacc tcttcaattc cgatttgatc tgcaatagca cgcgccacac cagcattgtc
     3901 tcctgtcagc atgactgttc ggagaccacg tttttttagc tgactgatgg ctagcttagc
     3961 attttcctta ggaatatctt gcaaagcaag caagcctttg atttcattgt caacagctaa
     4021 aaacacaact gtcttagctt ctttttctag ttcttctagt ttatcttgat aagtattaga
     4081 aatatccatg ccatccagca ttttagcatt tccaagtaaa acttgttttc cattgattcg
     4141 ccctgaaaca cctttcccgt gcaaggcctg aaaattttca acagtttgaa actcaagtcc
     4201 agcttcactc gctcgcttaa cgatagcctc agccagtggg tgttgagaag catcttccaa
     4261 ggaggctgcc aatccaaaca cttctacttc gtcgccgatg atatctgtta ccacaggttt
     4321 cccttccgtc aaagtcccgg tcttatcaaa gacaagagtt tgaactttct ggatttcctg
     4381 taagacagtt ccatttttga ggagaacccc catcttggca ctacgtcctg tccccaccat
     4441 aagggctgtc ggtgttgcaa gtcccaaggc acaaggacag gcgataatca aaaccgccac
     4501 tccgtagaga agagaggaca caaagctagc tccaagcacg accacactat ccctgagcaa
     4561 gacgaaccaa acccaaaagg tcatgattcc taaaatgaca actactggga caaaaatccc
     4621 tgaaatctta tccgtcaagt cctgaatcgg cgcacgactt gtctgagctt tcttcacaaa
     4681 atccacaatc tgagccaaaa cagtctctga gccaactttt tctgctctaa agacaagcgt
     4741 tccactatga ttgatggttg agccaatgac agtatctcca actgtcttgt ccacaggcag
     4801 actctcacct gtcaccatgg attcgtcaat actagagaca ccttctacta cgacaccatc
     4861 aacagcaatc ttttcaccgg gacgcactcg aatcaggtcg cctaccttga cttgttccaa
     4921 aggaacttgg acataactat catcactcaa gatttctgcg gttttagctt gcaagtccag
     4981 taatttctcc acagcttggg acgtattttt tctcattttt tcctcaaaaa ctgctcccaa
     5041 aagaacgaaa aagaggataa atccagcact ttcgaagtaa acagggagac cagcaaagag
     5101 agcaactagg ctatagaaat aagccactag agttcccagc gcaaccaagg tatccatgtt
     5161 ggcattgtgc tttttaaaac tggcccaagc actctggata tatggcttac ctgcaactaa
     5221 cataataggc gttgttgcta gaaaggttcc ccaatgcatg acttgatgac taatgctacc
     5281 tgtcaacatc ccaatcatga gaatcacaag aggcacagta aagatactag taatccaaaa
     5341 acgttgcagg agagatagag attttcgagt cttctcaacg actgtatagc ttcccttttg
     5401 catcttcatg ccacaagaaa attcatgtcg ccctaattct tgaggcgtaa aacgaatgac
     5461 tttctcctca tctacgccga ttggttccaa gataccttct tcttcaaaca gaatttcctt
     5521 ataacagttt gaaggagtag cacgatgaaa ggtaatctca gctggaattc ccttttgaag
     5581 ctggatatgg gctggatgat agcctttttc agctcggata cggatttttt gaatgccatt
     5641 ttctaagctt gctttcacaa tttctgtcat agtctccacc tactctacaa tcatcttgcc
     5701 gtgcatcatg ttcataccac aagcaaagcc aaactctcca gcctgttcag gcgtgatttc
     5761 cactacatac tcttccccca ttggcaggtt cgcatgtaca ccaaaatctg gaaaaacaat
     5821 ttgatccaga catggtgaag gatccttgcg gtcaaagaca atgcgtgctg gcactgattt
     5881 cttgaggaca atcaactcag gagtatagcc tcccatgact tccactcgaa tctcttggta
     5941 tccgtttttt tgctgggctt tttgtccaga tttttcaggc tttttgaaaa accaaaacaa
     6001 gataaacgcg ataagggcaa tacaaataat ggttacaata ctatttaaca tgacgtctcc
     6061 tttacataca attacatctt acttctgtta cagcacttga tttcttctct gaaatcacag
     6121 cttccaagtc ttccaagtca gtctgagtaa attcacattc tacaatcaag tcagccaaca
     6181 aattcctaat cctacgggaa caaaccttgt ctttgatatc ttggacaagt aaatcccgac
     6241 tttggtctag agttaaaagg gctgaataaa caaaggactt gccttctttt tttcgagtca
     6301 aacactcttt ctcaaccaga cgagccaaaa gtgtctgaac cgtggacttg gaccagtcaa
     6361 accgctctgc caaaacccta atcaaatctg tactggtctg ctccccctgc atccaaataa
     6421 tcttcatgac ctgccattct gcatctgaaa tctgcattac catacctcca aaatctacat
     6481 ttgtcaatta cactcatcag tatactctta aaatctacat ttgtcaatta tagaaataat
     6541 attttcttcg aaaaatagaa ttttaatcat ttgaaaaacg atttgcagtc aaatattact
     6601 atataaacaa taaaaatatg ctatactaaa gaaaaaagaa aacaaccact aggggtgcgt
     6661 aaagctgaga ttaacgactg ttagatccct ctgactcaat ctaggtaatg ctagctgatg
     6721 gaagtggaaa tgataatggg gactagcagt cttctattgc ctttctaaaa cagactagct
     6781 tgttcttaag aatacaaact tcagttggtt gggaggtttt agatgactta tttacccgtt
     6841 gctttgacca ttgcagggac tgaccctagt ggtggtgctg gcattatggc agatttaaag
     6901 tcattccaag cgagagatgt ctatggaatg gctgttgtaa ccagtcttgt cgctcaaaat
     6961 accagaggtg ttcagctaat cgagcacgtt tctcctcaaa tgttgaaagc ccaattggag
     7021 agtgtctttt ctgatattcc acctcaggct gtaaaaactg gaatgttggc tactactgaa
     7081 atcatggaaa tcatccaacc ctatcttaaa aaactggatt gtccctatgt ccttgatcct
     7141 gttatggttg ctacaagtgg agatgccttg attgactcaa gtgctagaga ctatctcaaa
     7201 acaaacttac tacctctagc aactattatt acgccaaatc ttcctgaagc agaagagatt
     7261 gttggttttt caatccatga ccccgaagac atgcagcgtg ctggtcgcct gattttaaaa
     7321 gaatttggtc ctcagtctgt ggttatcaaa ggcggacatc tcaaaggtgg tgctaaagat
     7381 ttcctcttta ccaagaatga acaatttgtc tgggaaagcc cacgaattca aacctgtcac
     7441 acccatggta ctggatgtac ctttgctgca gtgattactg ctgaactagc caagggcaag
     7501 agtctttacc aggcagttga taaggccaag gcctttatca caaaagctat tcaagatgcc
     7561 cctcaactcg gtcatggttc tggtccagtc aaccatacaa cttttaaaga ttaagaaaaa
     7621 aaactctcta gttcccactt taagggaatt agagagtttt tatactcttc gaaaatctct
     7681 tcaaactacg tcagcttcca tctgcaactt caaaacactg ttttgagctg acttcgtcag
     7741 tcttatctaa aacctcaagg cagtactttg agcaacctgc gactagcttt ctagtttact
     7801 ctttgatttt cattgagtat taattaggaa agaatgtcat gcaactttgt taaaaaggct
     7861 tgcgtttttg cctcaatatc ttctgcttgc atcaaatcac gtacaacagc tacaccagct
     7921 atgccagtgc ccataagctg atcaatattc tccgaagtca agccgccaat agcaactact
     7981 ggaatggcaa ccgtttgaca aattgttttc aaggttgaaa tcagggtgat gggtgcattt
     8041 tccttggtgg ttgtcggaaa aatggctcct gtccccaagt aatctgcacc tgatttctcc
     8101 gcttccagag ctcttttaac cgttttagcg gtgacaccga ggattttttc aggacccaag
     8161 acttgtcggg caaccgaaac tgggagttcg tcatctccaa tatgcagacc agcagcatca
     8221 accgcaagac aaacatccaa ccgatcatcg attatcaagg ggacctgata ggcatctgtt
     8281 atttccttga cttgttttgc cagttgataa tattgattgg ttgtgagatt tttttctcgc
     8341 aattggacta tggtaacccc tgaacggcag gccgtctcaa cttttgcaag aaagctttcc
     8401 acggaatctt gatagcgatt ggttaccaga tatagtctaa gtgcttctct attcataaac
     8461 ctctcctttg atggtatcta gccaattttc atctcttctt aggagcgaaa gctgattgag
     8521 tacttggtaa cgaaattctt ccaatcccat tccttgaaca actattttct cagcagcgat
     8581 attgagataa gagactgcta agcaagaagc ttcaaaacca gtctttcctt ggctgagaaa
     8641 aacggctgtt aaggctccaa ccaagtctcc tgtccctgtt atccagtcta attcagtaca
     8701 gccattctcc agtacagcaa cttgattctc cgaaacaata aggtccttgg ggccagtaac
     8761 taagaaagac ataccaggag aggtctgaca ccagtctttc aagacttgaa gcaaatcctc
     8821 cgtttcttga tctttagcac tcgcatcgac cccaacgccg tggtgcttta atccaacaag
     8881 acttcgaatt tctgacatgt ttcctttaag gaccgtaggt ctatagtcta aaaggtcttt
     8941 aactaagctc ttacgaatgg atgaagtcgt tacgccaacc gcatctacta ccatcgggag
     9001 agaagattgg tttgcatacg aagctgccat gcggattgct ttttccttct cagctgacaa
     9061 atgccccaaa ttgatgaaga gagcctgact ttgcttagta aaatcaagaa cttcacgggg
     9121 atcatctgcc atgacaggtt tgcatcccag agccaaaatc ccatttgcca gcatctcaca
     9181 agaaatctca ttggtcatac agtgaatgag ggaactagag cctataggaa aaggatttgt
     9241 caatgcctgc atcattctat cctttcagca aagaaatatc cttgcacttt tttaaagaat
     9301 tcctgcttga ttaaaaatct aaatgcaata aaggaaatcg ctgtaccaat caaggttgct
     9361 ccgaaaaatc gaggagtgta gataaaccaa ctaagcttag cagccgatcc tgtaaagagc
     9421 accataacag gataggaaac aatagaacca ataatacctg ttcccacaat ttctcccaag
     9481 gcagaaaagt aaaattttcg accgtactta taaaagagac ctgctagaag ggctccaaaa
     9541 gtcgctcctg tgagagctaa aggcggaatc ccttgagtcg tcatacggat aaaggctgtc
     9601 actgtagcca tagccaaggc ataaacaggt cccatcatga ttcccgctag aatattgact
     9661 acactggaca tcggtgccat tccctcaatc cgaaagatag gtgtaaggac tacatcaagg
     9721 gcaatcatca tagataaaat ggtcaatttg tgaacttgta gttggtgctt tctcatgttt
     9781 ctattcttct cctttttcta aagactgtaa atcgctcttc catgtctggt gttggtaagc
     9841 catctcccaa aacttggctt ccatatgaac actgatgtgg aaggcatcta gcattttttg
     9901 cttatctgtc tcatcacttt ctcgataaag ctgattgacc agtgctccct cctctctgat
     9961 ctgttgctct aactcatccg taatataagt ttcaatccat tgttgataga gaggatttgg
    10021 tgatggttta agattaagtg atttgcctat atcatggtat aaccaaggac aaggaagcaa
    10081 gcttgcaaaa gctatggcta agttcggttc tgcaaattgc ctataaatat gagaaatgta
    10141 atgataacag gttggagcga ttggatgttg ctccatttcc tggtcgttga tttccaattc
    10201 cttgaaaaat tgttggcgaa taaataactc accctccact aaaccctgag cattttgttt
    10261 caagagtctt ttcatctctt ggtttgaagt cttatcagcc aaaagatgat agatttctga
    10321 gaaagccttc agatagtagg catcctgaat caggtaatag cggaaaatgg caggttctaa
    10381 attcccctct tgtaattgta aaataaaggg atgatgaaag gaagcctgcc aagctttctt
    10441 ggataattcc atcgcaatat ctgtaaattc cataataact cctttataaa aatagactgg
    10501 tttgaagcaa taaaaagaaa agcaggtaga ttaattttgt ttttttagaa atataaaaag
    10561 tccgatagct attcttcaac tgtgcatgtt cgtcatatcc gtgagcagat agagctctca
    10621 ggtaaagatg gcgccaccta aagactgtca tcagaacctt actgtaaatc aagggcgacc
    10681 aaaaatgtag tttttgacca cgtaataggc aagcttcttt gagggacttg atttcttgct
    10741 gaatgagagg aaaagaattg aataccacaa tcaaggcata ggaccaagag cgtgatagcc
    10801 ccttttgagc caagtacaag agaagctctt ttagtgaaat agaggaaaca aagacaaggc
    10861 cgatacaaac tgtcacaaag gccctcgttc caagcatgac tgcctgtgaa gcatctccgt
    10921 gtaactgaac tgcccagtag ttggcaaaag atggtaaaat ggcaagtatg atcatccaag
    10981 ctaacatttt aaatcgacgg taatagagca taaagagaat acaaaatgcg actaccgaaa
    11041 gattcagagc aatcgaagga atgaaagatg tttccaagga taaaatcagc aagaagagac
    11101 tgataatcgg tgtctgggtt gctactttga ccatactatc tcacctcccc ttgggtattg
    11161 ctactctgag atgtaagtgg tttggtaatg gtcacttctt tcacatgccg aagaccctga
    11221 ctagtcatct caatccaata atcaaccaca gaaatcaaag ggtctaaacg atgactaatg
    11281 agcagaaaac ttcttccttg attcctctcc tccacaatcc acttgcaaaa ataatggcag
    11341 gctctatcat ccaaacctgc aaaaggttca tctagcaaga tcacggaagc cttactggtc
    11401 aagatggtca ggagctgaag aattttttgc tgaccaccac ttaattgata gggactctta
    11461 tcgactgcct gctccaaatc aaaatatcgt aaagcttgaa aaatccgctg atttctttca
    11521 gaatcaggtc catctaattg aagctcctct cgcagactga ctcggataaa ctgcttctca
    11581 gcttcctgaa caacacccgt cagatcacgg tacaaactct ttttcttttt caggactgtc
    11641 cccttccatg caatgcgccc cttatacttt tgaaattgaa gaatagaccg aaagagggtt
    11701 gatttcccga caccattgtc acccaggata caggaaatcc cttgatagaa tgtgaaatca
    11761 gcaatcgaaa agagggagcg atttccaaac tcacaagtcg ttctatccat atggaatagt
    11821 tccgggctag aagcaacttc ctttgaagca acctgtgtca tctcatagga agggatttga
    11881 aacacttccc ttagttttcc gtctcttagc tcaaccatat ggtcgatata ggctttatag
    11941 tcagataaat catggtcgca caaaataact gtcttcccat caagagccaa ctcttttaga
    12001 atctccaata tctcgattct gctcttgcgg tcaatggaag cgaagggctc atccaagaga
    12061 tagaccctag gattcatggc aaagaggaca gccagcgctg ctttttgctt ttccccacct
    12121 gataggtgat gaatgggacg gtgtaagatt gcctcgcaac gacattgctg gacaacctct
    12181 gctattttag aatcaatttc ctgaaggtga tagccgatat tctccaaggt gaaaatcaac
    12241 tcctcaaaca agttctccat ggtaaattga tgattgggat tttgcaagag aatgccaacc
    12301 ttctggacac gtgcgatgat agaaagctga ctgacctcgc tcccatctat caggacttga
    12361 ccactatagg gaagagaact gacttgggca atcatttgaa agaggctgga ttttcctgaa
    12421 ccactgctcc caactaacaa ggtaaaggct tgcgcatgaa aagtaaaatc aaacggctca
    12481 gagaagattg gggactgaat cgctcgtagt tccagaccca tctatgcttt tcctccagtt
    12541 gcaaactgat gatagagttt gacaatggca cgaaccaaga tggtacagaa gaaataaaca
    12601 gaaataaaac gtaccacaag caaggaaagg acaaacggaa gggaaaaggc gtagtaacct
    12661 aacttaatgt attcatagac aaagctaaca agcgtaatcc caatactatt agcagttaga
    12721 gagagccaac tttcatagcg attcttagtt acgataaaac caaattcact tcccaaacct
    12781 tgaaccaagc cagacaaaag agctcctaga ccaaattggc taccataaag gacttcagca
    12841 agcgcagcta gcacttctcc aatcgttgca cttccgactc tcggaacaaa gatggcagca
    12901 atgggcgcag ccatacacca gagaccgaag aggatttcat tagcaaaggc ctgcaaacca
    12961 agaggtgtta agagtagact gagaatatta tacacatatc ctgaaccaac gaaaacccca
    13021 ccaaaaaaga tagacaagaa agcaagcaag ataacatctt ttaactgcca ttttttcaac
    13081 ataaaaaact ccttttttta aagaaaagtg gggcactcaa gaagaccgac ctaaatactt
    13141 tgtatagcaa actgaattta gaacagtaca caagaa
//
LOCUS       DAVKJD010000049        13155 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKJD010000049 DAVKJD010000000
VERSION     DAVKJD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13155
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.049"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            1..1184
                     /locus_tag="VW157_001430"
                     /pseudo
     CDS             1..1184
                     /locus_tag="VW157_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(1269..2471)
                     /gene="rpsA"
                     /locus_tag="VW157_001431"
     CDS             complement(1269..2471)
                     /gene="rpsA"
                     /locus_tag="VW157_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9552715.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(2633..2704)
                     /locus_tag="VW157_001432"
     tRNA            complement(2633..2704)
                     /locus_tag="VW157_001432"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2670..2672),aa:Gln,seq:ttg)
     gene            complement(2714..2794)
                     /locus_tag="VW157_001433"
     tRNA            complement(2714..2794)
                     /locus_tag="VW157_001433"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2758..2760),aa:Tyr,seq:gta)
     gene            complement(2844..3074)
                     /locus_tag="VW157_001434"
     CDS             complement(2844..3074)
                     /locus_tag="VW157_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9552716.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(3242..3886)
                     /gene="pcp"
                     /locus_tag="VW157_001435"
     CDS             complement(3242..3886)
                     /gene="pcp"
                     /locus_tag="VW157_001435"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9552717.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(3901..4824)
                     /locus_tag="VW157_001436"
     CDS             complement(3901..4824)
                     /locus_tag="VW157_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9552718.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(4821..5510)
                     /locus_tag="VW157_001437"
     CDS             complement(4821..5510)
                     /locus_tag="VW157_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9552719.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(5575..6279)
                     /locus_tag="VW157_001438"
     CDS             complement(5575..6279)
                     /locus_tag="VW157_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9552720.1"
                     /translation="MVLSASAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIIDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            complement(6335..7357)
                     /locus_tag="VW157_001439"
     CDS             complement(6335..7357)
                     /locus_tag="VW157_001439"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9552721.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(7489..9960)
                     /gene="parC"
                     /locus_tag="VW157_001440"
     CDS             complement(7489..9960)
                     /gene="parC"
                     /locus_tag="VW157_001440"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9552722.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILSTSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEYK"
     gene            complement(10380..12323)
                     /gene="parE"
                     /locus_tag="VW157_001441"
     CDS             complement(10380..12323)
                     /gene="parE"
                     /locus_tag="VW157_001441"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9552723.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            12459..13100
                     /gene="plsY"
                     /locus_tag="VW157_001442"
     CDS             12459..13100
                     /gene="plsY"
                     /locus_tag="VW157_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9552724.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
BASE COUNT         3827 a         2970 c         2297 g         4061 t
ORIGIN      
        1 tcaaatgaag aaatatgact ttcaaaaacc ttctaaaatt ccttatcttg aaacaactgg
       61 tatgccttct agaattctcc ttagaaaacg ccgtttcaag tgctatcact gttcaaaaat
      121 gacggttgct gagactcccc tggtaaagaa aaatcaccaa atccctcgta tcatcaacca
      181 aaaaattgct caaaagttaa ttgaaaaaat ttctatgact aatattgccc atcagctttc
      241 catctcaact tcaactgtta ttcgtaagct caatgatttt cactttaaac atgatttttc
      301 ttgtcttcct gagattatgt cttgggaggt tgaaacagtc cggggagtga ctgtttcaat
      361 cgggagatgg agatgagctt cattgcgcaa gattttgata atcttaatat tatcactgtt
      421 cttgaaggca gaacacaagc tgtcatccga aatcactttc ttaaatatga tagagccgtc
      481 cgatgtcgcg tcaaaatcat tactatggat atgtttagcc cttactatga cttggctaaa
      541 cagcttcgct ttcaaatttc taggctcagg ctgaaacagt ctcccaggct gtttcactcc
      601 cgaatgctaa aatcgttctt gatcgctttc acattgtaca acatcttagc cgtgctatga
      661 gtcgtgtgca tgtccaaatc atgaatcagt ttcatcgaaa atcccatgaa tacaaggcta
      721 tcaagcgcta ctggaaactc attcaacagg atagccgtaa actgagtgat aagcgatttt
      781 atcgccctac ttttcgcatg cacttaacaa ataaagaaat tcttgacaag attttaagct
      841 attcagaaga cttgaaacac cactatcaga tctatcaact cttacttttt cactttcaga
      901 acaaagaccc tgagaaattt ttcggactca ttgaggacaa tctgaagcag gttcatccta
      961 tttttcagac tgtctttaaa acctttctaa agaacaaaga aaaaatcgtc aacgctcttc
     1021 aattacctta ttccaacgcc aaattggaag cgaccaataa tctcatcaaa cttatcaagc
     1081 gcaatgcctt tggttttcgg aactttgaaa acttcaaaaa acggattttt atcgctctga
     1141 atatcaaaaa agaaaggaca aaatttgtcc tttctcgagc ttagcttttt tcaacccact
     1201 acagttgaca aagagccaga aaaatccctt gtttagtaaa caaaggattt tgtgaatttt
     1261 caatttgatt aaagttcgat atcaccaaac aaatcagcca ttgaaaatcc tgtttgtgtt
     1321 tctggaagtt cgaaatcacg cttttcttga cgtcttggac gacgtggacg agcagcacgt
     1381 ttttcttctt tttgtccttc ttcttgggct ggacgttctt caagagcttt aatagaaagt
     1441 gacacgcgtt ctgcatctgc gttaacttca agaactttaa cttgaacttc ttgaccaact
     1501 ttaagagctt cttttggatt ttcaatccgt ttgtgtgaaa tttgtgatac gtgaacaagt
     1561 ccatcgatac ctggcaatac ttcaacaaat gcaccgaagt cagtcaaacg tttaactgtt
     1621 ccttctacta catcaccttt agccaatttt tgctcaacgc catcccatgg tcctggtact
     1681 gttgctttaa gtgaaagtga tacacgtcct tcttcttcgt taagatcaag gattttcact
     1741 tcaatttctt caccaacagt tacaactgat tttggtgata cgttacgttc atgtgacaat
     1801 tcagtcaagt gaaccaatcc atcaacacca ccaaggtcga tgaaagcgcc gaagcttgtg
     1861 atacgagcaa ctttaccagt tacaacatca ccaacagcca atttaccgaa tacttcagcg
     1921 cgagctgctg cagtagctgc ttcaacaact tcacgacgtg aaaggatgaa gcggttttct
     1981 ttagcgttaa cttctttgat tttagtatca aattcttgac ctacaaaacg ctcagcgtta
     2041 cgtacgaaac gagtatccaa cattgaagct gggataaatc cacgaacacc ttcaaattct
     2101 actgaaagtc cacctttaac ggcacgcgtt cctttaacag taacaacttc ttcttcgcga
     2161 ccaacaagtt tgtcccatgc tttgcgagct tcaaggcgtt ttttagatac aaggtatgta
     2221 actgtatcag tatctttacc aactacttga cgaagtacaa gaacatccaa tacttctcct
     2281 actttaacaa agtcattgat atctgcatca cgatcgtttg tcaattcgcg aagagtcaag
     2341 acaccttcaa caccagttcc agagattgca acgttagctt gagtcgcatc aactgtcaat
     2401 acttcagcac taacaacatc accagtctca acttgactaa cgctatttag caaatcttca
     2461 aattcgttca tctaaaaaat cctccaacaa tcaagttttt cttaaacttg acatacttat
     2521 aatttttttc ctaagcaccc gcaaggacat gttcatatca ttcacgtctt tcaattataa
     2581 aaataaaata ccctaagata ttttgctttc tatcttgatt attacctaag aactggggta
     2641 gctggattcg aaccaacgca tgagggagtc aaagtccctt gccttaccgc ttggctatac
     2701 cccattaatg aaatggagag agagggattc gaacccccga acccgaagga gcggatttac
     2761 agtccgccgc gtttagcctc ttcgctatct ctcctacaat caacatggac tattatatca
     2821 tgaaaatttc aaaaaaacaa gacttatttt gctaaattat aattttcaat caaaatttcc
     2881 acagcttttt ccaatttttt ataaaaacta tcgacattcc cattctttat aatggcaaat
     2941 tgtccgtcta attcggcaat ttctccgata acttttttac cgatagtcaa tgtataacct
     3001 tcaaaactgt ctttaccaac attaactttg gcatccgcta cttgaatttc gattttttta
     3061 tctttcttgc tcatttcata cctctcttca ctttttctat tttacaaaaa aatcctaaaa
     3121 ctagcaagaa aaaactctat atcaggcttc tctgatatag agttttatgt aaacaacctc
     3181 aattgattta gcatagttaa tagttagtaa acaagcataa ttgactatcc tttttaggtt
     3241 atcagtgagt ctcaccgcct actaactgca aatccttatc tttgtagtct acaatagctt
     3301 caatagctac ctctatgcct cttacaatat cacatagact cattcctgca gtattcggct
     3361 tatttactac ttgttccatc atatatggaa tatgcatgaa acctgctctc atattaggga
     3421 attttttatc cactaaataa agagcttggt acatcaaatg attgcaaaca aaggttcctg
     3481 cactattgga tacaactgcc ggaagtccct gttttttgat agcttgtacc atcgctttga
     3541 taggtaaact actaaaatag gccgatgctc catcaatacg aatcggtgta tcaattggtt
     3601 gattgccttc gttatcaggt atgcgagcat catcttgatt aatagccact cgttcaggtg
     3661 ttaagccggt cctgccgcct gcttgtccaa tacaaagtac agcatctggt tgatatcgta
     3721 atatttctgc ctctaaaact tctgacgact tataaaaaac cgttggaatt tctacccagc
     3781 gaacttcagc cccattaatc tcagatggta ataattttac agcctccaaa gctggattaa
     3841 tcttttcacc tccaaaagga ttaaaacctg taaccaatat tttcatttta ttttccttta
     3901 ctaaaatgcg agaaagtaca ttaagaatat gtgaataaca atcattacta gagcaacacc
     3961 tgcttgagcc tttataacgc cattctgatc tttcatatcc atcaatgctg ctggtagagc
     4021 gttaaaatta gcagccattg gggtcaataa ggtcccacaa taacctgctg tcatggcaag
     4081 agcaccagcc acaattggat tagctcccag agcaaataca aagggaactc caacacctgc
     4141 tgtaataacg gtgaatgctg caaaagcatt tcccataatc attgtgaata gaaccattcc
     4201 aagaacatag gccaaaactc ctataaagcg actatctgaa ggaacaatac cgctaatcag
     4261 atgagagata acatcaccaa cacctgctac agtaaaaata gcccccaaag cccctaataa
     4321 ttgaggaaca atcccacttg ttgaaacttg ctgagtcatt cgattatttt ctgataacag
     4381 actcttaggg tgactattgg taatcacaag aacagaaatt gtagcaaaca aggcggcaag
     4441 gctaatcgaa atcttgctaa attctggaat catttgcgct aagaccaacg caagtattgc
     4501 catcagcata actggaataa aaattttatt tttcaacctg ttagattcaa tattggcttt
     4561 catttcatct aaggatggca aggttccgat acggacttgc ttaaacaatg ttaacagcga
     4621 taataggatt acaataatac caatactcat atttggcata taggaaccac ctataaacgt
     4681 aatagacaat agagtccaaa atgcagatgt cccaagtcga actgggtttg ttttatcttt
     4741 ataactacaa taggctgtat ggagaaattg acaaccaatc acaatatagg tcaactctaa
     4801 tagttgcttt gccaactctg tcatttttgt tctcctcccc tagtcttttt tgatatcaat
     4861 tttttatcaa ataaataatt gtaaatcccc actacaataa gtgttataac agcaacaata
     4921 atagatgtag aagcaatccc tgcataattg ctttcatagc ctaactgatc taatgttccc
     4981 cctatcaaga ggactccccc agcacctaca aacgtatttt gagcaaagaa atttccaaaa
     5041 ttttcattcg cagccgcacg cgcttttatt gtctcatctt caacctctgt taactttcta
     5101 cctaattgag actctgcagc tgcttctccc ataggttgaa ccaaaggtct gacaaactga
     5161 gggtggcctc ctagacgaat tgaaaagaaa ccagctaact ctcgaataaa gaaataaact
     5221 gtatagaagt ttccaactgt cagaccttta atctttcgaa tcaaatcgat tgatcgttgc
     5281 ttgagtccaa aggtttctga cagccccaca agaggcaagg taaccataaa aatcgtgagc
     5341 actcgctgat tgctaaattc ttttcccaaa atctccaaaa attcaacgag agaaacacct
     5401 gaaactaaag ctgtaaccaa accagctaag actactgttg caattgtatc aaattttaaa
     5461 ataaaaccca caacaatgat tgctattcct attaatctaa tccactccat atcaaactcc
     5521 tttatattca aaatgacagt atttttaaaa ttttatcaag atcaatacca ttccttattt
     5581 aatgtgtttt tctagttctt tttggtattt gctattggat tccaattttt ctttttgcca
     5641 ttttttaaaa acctcgttat attcttttgt tgtaacaata tctttttgca atttcattcc
     5701 tttaaagata tatggatccc ccttaatacc aacttgtgag tatggttttg agaatggtac
     5761 tacgttactt acaactggag aaccaccaga tgaagctgtt ggcatcaata atgaactatc
     5821 tgtcgaccaa gcttgagctt tggcatattt ttcatatctt ttctctaggt cagtggtctc
     5881 agaaacagca tcttctaaca atttcttata tttatccaaa ccaggtttag ctacaacatc
     5941 cttatctttt cctttcgtaa taccaaggtg tttcatggca gaaccagatt ttggatctat
     6001 aatattcaag taagacgctg gatcttgata gcttggagcc catcctgtac tattcaaatc
     6061 atagtctttt tgagaaggag caacattgcc gtatttatca ttttccatca aaccatcaat
     6121 aacatttcca ataacgtctg tcgtcgatgt tcgagtcgct atactgtagc ccaatgatgc
     6181 tggatctact gcatagacat aagaaaatgt tgtcggtgca tctgcttctt tatcagtttt
     6241 tccacaagcc actaaaatag ctgacgcgct caggaccact cctgctgtta agagccactt
     6301 tttctatttc ataaaaaatc tcctttggtt tattttaatc tacttttaca atccaacctt
     6361 ctggcgcttc aatatcgcca aactgaatac ccgtcaattc attatataat ttacgcgtca
     6421 caggacctac ttctgtttca ctatagaata catggaaatc atcaccatgt tgaatacctc
     6481 caattggaga aataaccgct gctgtaccac aggcacctgc ctctacaaaa cggtcaagat
     6541 tatcaattgg aacatcaccc tcaataggag ttaatcccaa gcgatgttct gccaaataaa
     6601 gcaaggaata cttggtaata gatggcaaga tagatggact caatggtgtt acaaattcat
     6661 tatcagctgt aattccaaag aagttagctg atccgacttc ttcaatcttt gtatgagttg
     6721 atgggtccag atagataaca tctgagaaat gacgtgactt ggccattttt cctggtaaga
     6781 gacttgcagc atagtttcca ccaaccttag ccgcacctgt accatttggt gctgcacggt
     6841 cgtactcatc ctgaatcaag aagttggttg ggaccaaacc acctttaaag taatttccaa
     6901 ctggcatagc aaagatggtg aaaatgtact cttctgccgg ttttaccccg ataatatctc
     6961 cgacaccaat caaaagaggg cgaagatata aggttccacc tgttccgtat ggtggtacgt
     7021 attcttcatt cgcacggaca actgctttac aagcttctac aaacatgtct gtcggaactt
     7081 gtggcatcaa gagacggtca catgtacgtt gcagacgttt agcattttca tcaggacgga
     7141 acagttgaac actgccatcc ttagtacgat aagctttcaa accttcaaat gcttgttgtc
     7201 catagtgaag acttggagaa gactctgaaa tatgcaaagt tgcatcctct gtaagctctc
     7261 cttgatccca ttgtccattt ttgaaatgag caagatagcg ataaggtaat ttcatatagg
     7321 aaaaaccgag gttttcccaa tcaatcgtta ctgtcatatt ccactcctta ttctaaaaac
     7381 ctatttctta tattctacac tatttttcta aaatagcaag tatattttgt aattttcaga
     7441 aaatttctcc aataaaaacc agctcttaga actgattctt catttcactt atttatattc
     7501 agtaactact tcctgaagat aagcgtcaaa aacttcttca tctgaaatcg tgtcagaaat
     7561 gaagcttcca ttgctagtgc gttctgacaa gttcaagtct tgcaatcggc tttcatagat
     7621 tgttccttta ttggattgga caagcagagt ttggtcgttc acatctactt ccgtactgaa
     7681 gaaatcgcca acaaatcctt gctctgcaac tgctcctgcc aagaagacac gatgcggttt
     7741 gtttttcaac tcacgcaaga cttgtaatcc tcgtttggca cggctggttg atagaatttc
     7801 ctcaatggaa acacgtttca agcttccacg ctgggtcaag aggtagaagg acgaagtatt
     7861 acagataaag ccagattgga ggacatcatc ttctttcaaa ttcatagcct tgacacctgc
     7921 tgccttagca ccgacaaccg gaacctcttc gatattgaaa cgcagggcat aaccattttg
     7981 actaaccaag acaacatcat ctagtttaat cggagccact gctacaatct gatctgtatc
     8041 gtctttgagc ttagcatact tgacagactt agatctatag gtccgccatg gagtgaattc
     8101 ttttcgctct acccgtttga tttgaccaag gcgagtcact gcaaagtagg ttgtcgcatc
     8161 gtcaaactga tccagtactt ccacataaag gatttcttca ttcgtttcaa agtttgtgat
     8221 ggtttggctc agatgctctc cgatgtcctt ccaacgaata tctgccaact catggattgg
     8281 tctgtagatg acatttccaa gacttgtgaa catcaagagg tgctgggttg tcttggcaga
     8341 ttgaacaaaa atcaaacggt catcatcacg cttgccaatt tcttccaagg tggaagccgc
     8401 aaaggaacgt ggactggtac gcttgatgta acctgccttg gtcacgctga cgtaggtatc
     8461 ttcctcagcg ataagactag ctgtatcaat ctcaattgct ttcgcagtat cttctaaaga
     8521 actcaaacga ggagttgcaa atttcttctt gacctcacga agttctttct tcatgagatt
     8581 gtacatagtc ctttcatcac cgataatagc cgccagcata gcaatcttct cacgaagctc
     8641 tgcttcttct tcctgcaaga caaccacatc ggtattggtc aaacggtaca gttgcaaagt
     8701 tacgatagcc tcagcctgtt cttccgtaaa atcatagcta actttgaggt tttccttggc
     8761 gtccgcctta ttctcagaag cacggataag agcaatgact tcatccaaaa tcgaaatcac
     8821 acgaatcaaa ccttcgacga tatggagacg tttctcagcc ttttctttgt caaagcgtga
     8881 acgcgccaaa atcacttctc gacggtgagc gatgtagcta gacaggattg gaacaatccc
     8941 aacctgacga ggtgtgaaat tgtcaatcgc caccatatta aagttgtagt tgatttgtag
     9001 gtcggtgtac ttaaataagt agttgagaac aagctcagta ttagcgtctt tcttaagttc
     9061 gatagcgata cgaagaccat cacggtcaga ctcatcacga acctcagcaa tcccagctac
     9121 cttgttatta acacgaacat catcgatttt cttgactaga ttggccttat tgatttcata
     9181 aggaatctca gtaataacga tttgttcctt accacctttt agcttttcaa tttcagtctt
     9241 ggaacgaaca accacgcgcc ctttcccagt ctcataagct ttcttgattt catcacgacc
     9301 ctgaataata gcccctgtag ggaagtctgg tccaggcaag aattccatga gtttatcaat
     9361 ctttgcagtt gggtggtcaa tcatgtaaac tgcagcatct atgacctcag ctaaattatg
     9421 gggaggaatg tctgtggcat aaccagccga aatcccagtc gaaccattga ccaagaggtt
     9481 tggaaaggct gctggcaaga ccgttggttc tttctccgta tcgtcaaagt tccatgcaaa
     9541 aggaactgta tttttctcga tatcctgaag aaggtagcct gcaatttcag acaaacgtgc
     9601 ctcagtataa cgcatagccg caggaggatc tccgtccata gaaccgttat taccgtgcat
     9661 ttcaactaga atctcacgat ttttccagtt ctgtgacata cgaaccatgg catcatagat
     9721 agaagaatcc ccgtgtgggt ggaaattccc catgatgttc ccgactgact tggccgactt
     9781 acggtagctc ttgtcaaaag tattgctatc cttattcata gaataaagaa tacggcgctg
     9841 aaccggcttc aacccatcac gaatatctgg caaagcccgg tcttgaataa tgtacttgga
     9901 gtagcgacca aagcgctctc ccatgatgtc ctccagggac atgttttgaa tgttagacat
     9961 aagatacaaa gcccataaaa taccaagtga aaatagaaaa ttcttgaagt aagcaaactc
    10021 acaagagaat ttatcttttt cacacagtat ctagggcgtg ttcaactcct ttcaaagaat
    10081 gtagagtagg tttttatgca gtaaaagata ttttacggga attcctcccg tgttcagtta
    10141 cgataagtaa ccaaactatc ctgtttgtat ttttcaatat gaaaatctgg ttttccaaaa
    10201 ttagtcttag tttgtgtctt agccgctccc ttaagcgcct ctttgagata agcactcata
    10261 gcagattctt cattaataat cctgcaattt tttcaaacca agattttcaa actgcttttt
    10321 cacatagtca ttcacatccg actctaattt ccagtttact aacatattat tttctttcat
    10381 taaaacactg tcgcttcttc tagcgtaaac ttgacattat cttcaatcca tttacggcgt
    10441 ggttctacct tatctcccat gagaacattg acgcggcgtt cggcgcgcgc taaatcttca
    10501 attgtgacac ggatgagggt acgtgtttct gggttcatgg ttgtttccca gagctggtcc
    10561 gcattcatct caccaagtcc tttgtatcgt tggagggtag cgcctttacc gaactgttta
    10621 cggagttctt ctagttctcc gtccgtccaa gcgtaggcca cttcttcttt cttgccttta
    10681 cctttggaca tcttgtaaag aggtgggagg gcaatataga catgacctgc ctcgactagc
    10741 ggacgcatgt aacggtagaa aaatgtcaag agcaaggtct ggatatgggc accgtcggta
    10801 tccgcatcgg tcatgataat gatcttatca tagttggcat cttcaataga gaagtctgct
    10861 ccaacacccg caccaatggt ataaatcatg gtattgatct cttcattttt gaggatatcc
    10921 gccatcttgg ccttggctgt attgataacc ttaccacgaa gagggagaat agcctggaac
    10981 ttgcggtcac gaccttgttt ggcagaacca ccggcagagt ccccctcaac tagatagagt
    11041 tcattcttag caggattctt agattgggct ggggtcaatt tcccagacaa caagccctta
    11101 tctttcttgt ttttcttccc atttcggctc tcatcacgcg ccttacgtgc tgcttcacga
    11161 gcatcacggg ccttgatagc cttgcggatg aggttagaag ctaattcccc attttccata
    11221 aggaaaaagg tcaacttatc agccactatt ccatccacaa ctgggcgagc tagggggctt
    11281 cctagtttat ccttggtctg tccttcaaac tgcaagtgtt cttcaggaac taagatagaa
    11341 agaacggccg ctagtccctc acgatagtct gaaccttcaa ggtttttatc tttttccttg
    11401 agaagacctg ttttacgtgc atagtcattc atgaccttgg taatggcaga cttgagtcct
    11461 gtctcgtgcg ttccaccgtc cttggtgcga acgttattga caaaggatag aatgttatct
    11521 gagaatccgt cattgtactg gagggctact tccacttgaa aaccattgtc ttccccttca
    11581 aagtaaagaa ctggcgtcaa gatttcctta tcttcgttga gataagaaac aaaatcttgt
    11641 actccattct catagtggaa ctcaatcgct tcatctgttc gcttgtccgt taaagacaag
    11701 gtcacatttt tcaagagaaa ggctgattca ttaaggcgct ctgaaatggt attgtacttg
    11761 aaatctgtcg tagaaaagat agtcgcgtca ggcataaaag taactttggt gcctgtttta
    11821 gacttgggtg ctgtaccgat tttcttcaaa gtcgtgacag gttttccacc attttcgaaa
    11881 cgttgcttgt aaactgcgcc atcacgggta atttcaactt ctaaccagct agaaagggcg
    11941 ttaacaacgg aagaacccac tccgtgaagt ccacctgatg tcttatagcc accttgaccg
    12001 aatttccctc cggcatgaag aatggtaaag ataacctcaa cagttggaat tcccatagcg
    12061 tgcatacctg tcggcatccc acgtccatgg tcttgaaccg ttagactacc gtctttattg
    12121 atggttacat cgatacgatc accaaaccca gacaaggctt catcgactgc attatcaacg
    12181 atttcccaaa ctaggtgatg aagaccagcg ccatcggtcg atccaatata catccctgga
    12241 cgttttcgga ccgcatccaa cccttctagc acctgaatag catcatcatt ataattgtta
    12301 atattgattt ccttttttga cacaaggaac ctcctattcg ttcatcttta ctattctaca
    12361 ggttttccaa ggattttgca aaatttttct ttctccgatg tgacaatttt agcagagatt
    12421 ctctgctttt ctttcccaat tcatgatata ataggagtat gattacaata gttttattaa
    12481 tcctagccta tctgctgggt tcgattccat ctggtctctg gattggacaa gtattctttc
    12541 aaatcaatct acgcgagcat ggttctggta acactggaac gaccaatacc ttccgcattt
    12601 taggtaagaa agctggtatg gcaacctttg tgattgactt tttcaaagga accctagcaa
    12661 cgctgcttcc gattattttt catctacaag gcgtttctcc tctcatcttt ggacttttgg
    12721 ctgttatcgg ccataccttc cctatctttg caggatttaa aggtggtaag gctgtcgcaa
    12781 ccagtgctgg agtaattttc ggatttgcgc ctatcttctg tctctacctt gcgattatct
    12841 tctttggagc tctctatctt ggcagtatga tttcactgtc tagtgtcaca gcatcgatcg
    12901 cggctgttat cggggttctg ctctttccac tttttggttt tatcctgagt aactatgact
    12961 ctctcttcat cgctattatc ttagcacttg ctagtttgat tatcattcgt cataaggaca
    13021 atatagctcg tatcaaaaat aaaactgaaa atttggtccc ttggggattg aacttaaccc
    13081 atcaagatcc taaaaaataa aatgccagtt ctgtgaactg caccccaaaa agttagacaa
    13141 ataatttatc cgaag
//
LOCUS       DAVKJD010000050        12994 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKJD010000050 DAVKJD010000000
VERSION     DAVKJD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12994
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.050"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            24..1160
                     /gene="dnaN"
                     /locus_tag="VW157_001443"
     CDS             24..1160
                     /gene="dnaN"
                     /locus_tag="VW157_001443"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9552725.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQYKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTEQVTGKDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDENFMQLITPVRTN"
     gene            1225..1419
                     /locus_tag="VW157_001444"
     CDS             1225..1419
                     /locus_tag="VW157_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9552726.1"
                     /translation="MYQVGNFVEMKKPHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKINKIID"
     gene            1503..2618
                     /gene="ychF"
                     /locus_tag="VW157_001445"
     CDS             1503..2618
                     /gene="ychF"
                     /locus_tag="VW157_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9552727.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2689..3258
                     /locus_tag="VW157_001446"
     CDS             2689..3258
                     /locus_tag="VW157_001446"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9552728.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSIDKVDDSVNYYLQEKKFEKTMQRYNG"
     gene            3259..6768
                     /gene="mfd"
                     /locus_tag="VW157_001447"
     CDS             3259..6768
                     /gene="mfd"
                     /locus_tag="VW157_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9552729.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKEIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQGYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            6826..7092
                     /locus_tag="VW157_001448"
     CDS             6826..7092
                     /locus_tag="VW157_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9552730.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7085..7453
                     /locus_tag="VW157_001449"
     CDS             7085..7453
                     /locus_tag="VW157_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9552731.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7573..8841
                     /locus_tag="VW157_001450"
     CDS             7573..8841
                     /locus_tag="VW157_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9552732.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8838..10115
                     /gene="tilS"
                     /locus_tag="VW157_001451"
     CDS             8838..10115
                     /gene="tilS"
                     /locus_tag="VW157_001451"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9552733.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDEFVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10119..10661
                     /gene="hpt"
                     /locus_tag="VW157_001452"
     CDS             10119..10661
                     /gene="hpt"
                     /locus_tag="VW157_001452"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9552734.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10677..12635
                     /gene="ftsH"
                     /locus_tag="VW157_001453"
     CDS             10677..12635
                     /gene="ftsH"
                     /locus_tag="VW157_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9552735.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            12757..>12994
                     /locus_tag="VW157_001454"
     CDS             12757..>12994
                     /locus_tag="VW157_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9552736.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKR"
BASE COUNT         4484 a         1947 c         2654 g         3909 t
ORIGIN      
        1 taaaaataaa taaaggagaa tccatgattc atttttcaat taataaaaat ttatttctac
       61 aagcattaaa tactactaag agagctatta gttctaaaaa tgccattcct attttatcaa
      121 cagtaaaaat tgacgtgacc aatgaaggta ttactttaat tggttcaaat ggtcaaattt
      181 caattgaaaa ttttatttct caaaaaaatg aagatgctgg tttgttaatt acttctttag
      241 gttcgatcct tcttgaagct tctttcttta tcaatgtagt atctagttta cctgatgtaa
      301 ctcttgattt taaagaaatt gaacaaaatc aaattgtttt aaccagtggc aaatcagaaa
      361 ttaccctaaa aggaaaagat agcgaacaat atccacgaat ccaagaaatt tcagcaagca
      421 ctcctttaat acttgaaaca aaattactca agaaaattat taatgaaaca gcctttgctg
      481 caagtacaca agagagtcgt ccgattttaa caggtgtcca cttcgtattg agtcaataca
      541 aagagttaaa aacagttgca acagactctc atcgcctaag ccagaaaaaa ttgactcttg
      601 aaaaaaatag tgatgatttt gatgtcgtaa ttcctagccg ttctctacgc gaattttcag
      661 cggtatttac agatgatatc gaaactgtag agattttctt tgccaataac caaatcctct
      721 ttagaagcga aaatattagc ttctatactc gtctcctaga aggaaactat cctgatacag
      781 atcgcttgat tccaacagac tttaacacta ctattacttt taatgtggta aacttacgcc
      841 agtcaatgga gcgtgcccgt cttttatcaa gtgcgactca aaatggtact gtgaaacttg
      901 aaattaagga tggggttgtt agcgcccatg ttcactctcc agaagttggt aaagtaaacg
      961 aagaaatcga tactgaacag gttactggta aagatttgac cattagtttc aatccaactt
     1021 acttgattga ttctcttaaa gctttaaata gcgaaaaggt gaccattagc tttatctcag
     1081 ctgttcgtcc atttactctt gtgccagcag atactgacga aaacttcatg cagctcatta
     1141 caccagttcg tacaaattaa gtgaaagagg ttgagcctgg ctcgcctctt ttatgatata
     1201 atcgaaaaag aaaaggagag tagtatgtat caagttggaa attttgttga gatgaaaaaa
     1261 ccacacgctt gtacaatcaa gtcgactggt aaaaaggcta atcgttggga aattacacgt
     1321 gtaggagcag atatcaaaat aaaatgcagt aattgtgagc atgttgtcat gatggggcga
     1381 tatgattttg agcgaaaaat aaataaaatt attgactgag aacccttagt tagagggtta
     1441 gcactttatc tctttttgtg ttataatatt agggattgaa atgaaaacgg agaatgagaa
     1501 atatggcttt gacagcaggt atcgttggtt tgccaaacgt tggtaaatca acactattta
     1561 atgcaattac aaaagcagga gcagaggcag caaactaccc atttgcgacg attgatccaa
     1621 atgttggaat ggtggaagtt ccagatgaac gcctacaaaa actaactgaa atgataactc
     1681 ctaaaaagac agttcccaca acatttgaat ttacagatat tgcagggatt gtaaaaggag
     1741 cttcaaaagg agaagggcta gggaataaat tcttggccaa tattcgtgaa gtagatgcga
     1801 ttgttcacgt agttcgtgct tttgatgatg aaaatgtaat gcgcgagcaa ggacgtgaag
     1861 acgcctttgt ggatccactt gcagatattg acaccattaa cctagaattg attcttgcag
     1921 acttagaatc agtcaataaa cgatatgcgc gtgtagaaaa gatggcacgt acgcaaaaag
     1981 ataaagaatc agtagcagaa ttcaatgttc ttcaaaagat taaaccagtc ctagaagacg
     2041 ggaaatcagc tcgtaccatt gaatttacag atgaggaaca aaaggttgtc aaaggtcttt
     2101 tccttttgac gactaaacca gttctttatg tagctaatgt ggacgaggat gtggtttcag
     2161 aacctgactc tatcgactat gtcaaacaaa ttcgtgaatt tgcagcgaca gaaaatgctg
     2221 aagtagtcgt tatttctgcg cgtgctgagg aagaaatttc tgaattggat gatgaagata
     2281 aaaaagagtt tcttgaagcc attggtttga cagaatcagg tgtagataag ttgacgcgtg
     2341 cagcttacca cttgcttgga ttgggaactt acttcacagc tggtgaaaaa gaagttcgcg
     2401 cttggacttt caaacgtggt atgaaggctc ctcaagcagc tggtattatc cactcagact
     2461 ttgaaaaagg ctttattcgt gcagtaacca tgtcatatga agatctagtg aaatacggat
     2521 ctgaaaaggc cgtaaaagaa gctggacgct tgcgtgaaga aggaaaagaa tatatcgttc
     2581 aagatggcga catcatggaa ttccgcttta atgtctaaaa attaataaat ggtgtcaatt
     2641 aggttggaaa aaaattccaa cccttttggc ttttgaaagg aaaaataaat gaccaaatta
     2701 cttgtaggtt tgggaaatcc aggggataaa tattttgaaa caaaacacaa tgttggtttt
     2761 atgttgattg atcaactagc gaagaaacag aatgtcactt ttacacacga taagatattt
     2821 caagctgacc tagcatcctt tttcctaaat ggagaaaaaa tttatctggt taaaccaacg
     2881 acctttatga atgaaagtgg aaaagcagtt catgctttat taacttacta tggtttggat
     2941 attgacgatt tacttatcat ttacgatgat cttgacatgg aagttgggaa aattcgttta
     3001 agagcaaaag gctcagcagg tggtcataat ggtatcaagt ctattattca acatatagga
     3061 actcaggtct ttaaccgtgt taagattgga attggaagac ctaaaaatgg tatgtcagtt
     3121 gttcatcatg ttttgagtaa gtttgacagg gatgattata tcggtatttt acagtctatt
     3181 gacaaagttg acgattctgt aaactactat ttacaagaga aaaaatttga gaaaacaatg
     3241 cagaggtata acggataaat ggtgacctta ttagatttat tctcagaaaa tgatcagatt
     3301 aaaaaatggc atcaaaattt aacagataag aaaagacaac taatacttgg tttatcaaca
     3361 tctactaagg ctcttgcaat tgcaagcagt ttagaaaaag aagataggat tgtgttattg
     3421 acgtcaactt atggagaagc agaaggactt gttagtgatc ttatctctat cttgggtgag
     3481 gaactcgtct atccattttt ggtagatgat gctcctatgg tggagttttt gatgtcttca
     3541 caggaaaaaa ttatttcacg ggttgaagcc ttgcgttttt tgactgattc atctaagaaa
     3601 gggattttag tttgtaatat cgcagcaagt cgattgattt taccgtctcc caatgcattc
     3661 aaagatagta ttgtaaaaat ctcagttggt gaagaatatg atcaacacgc gtttatccat
     3721 cagttaaagg aaaatggcta tcgaaaagtt actcaagtac aaactcaggg cgaatttagt
     3781 cttcgaggag atattttaga tatttttgaa atatcccagt tagaaccttg tcgaattgag
     3841 ttttttggtg atgaaattga tggtatcagg tcatttgaag tagaaacaca attatcgaaa
     3901 gaaaataaga cagaactcac tatctttcca gctagtgata tgcttttgag agaaaaggat
     3961 tatcaacgag gacagtcagc tttagaaaaa caaatttcaa aaactttatc acctattttg
     4021 aaatcatacc tagaagaaat tctttcaagt tttcaccaaa aacaaagtca tgcagactct
     4081 cggaagtttt tatctttgtg ctatgataag acatggactg tctttgatta tattgaaaaa
     4141 gatactccaa tattctttga tgattatcaa aaattgatga atcagtatga agtctttgaa
     4201 agagaattag cgcagtactt tacagaagaa ttacagaata gtaaagcatt ttctgatatg
     4261 cagtattttt ctgatattga acaaatctat aaaaaacaaa gtccagtgac ctttttctct
     4321 aatcttcaaa agggtttagg aaatctcaaa tttgacaaaa tttatcaatt caatcaatat
     4381 cctatgcagg aatttttcaa tcagttttct tttctaaaag aagaaattga acgatataaa
     4441 aaaatggatt acaccattat tctgcagtct agcaattcaa tgggaagtaa aacattggag
     4501 gatatgttag aggaatatca gattaaattg gattctagag ataagacaag tatctgtaaa
     4561 gaatctgtaa atttaataga gggtaatctc agacatggtt ttcattttgt agatgaaaag
     4621 attttattga taactgaaca tgagattttt caaaagaaat taaagcgtcg ttttcgaaga
     4681 caacatgttt caaatgcaga gagattaaaa gattacaatg aacttgaaaa aggggactat
     4741 gttgtccatc atatccatgg gattggtcaa tatctaggaa ttgaaaccat tgaaatcaag
     4801 gaaattcatc gtgattatgt cagtgtccag taccaaaatg gtgatcaaat ttctattccc
     4861 gtggaacaga ttcatctact gtccaaatat atttcaagtg atggtaaagc tccaaaactc
     4921 aataaattaa atgacggtca ttttaaaaag gccaagcaaa aggttaagaa ccaggtagag
     4981 gatatagctg atgatttaat caaactctac tctgaacgta gtcagttgaa gggttttgct
     5041 ttctcagctg atgatgatga tcaagatgcc tttgatgatg ctttccctta tgttgaaacg
     5101 gatgatcaac ttcgtagtat tgaggaaatc aagagggata tgcaggcttc tcagccaatg
     5161 gatcgacttt tagttgggga tgttggtttt ggaaagactg aagttgctat gcgtgcagcc
     5221 tttaaagcag tcaatgatca caaacaggtt gtcattctag ttccgacgac ggttttagcg
     5281 caacagcact atacgaattt taaggaacga ttccaaaatt ttgcagttaa tgttgatgtg
     5341 ttgagtcgct ttagaagtaa aaaagagcag actgcaacac ttgaaaaatt gaaaaacggt
     5401 caagtcgata ttttgattgg aacacatcgt gttttgtcaa aagatgttgt gtttgctgat
     5461 ttgggcttga tgattattga tgaggaacag cgatttggtg tcaagcataa ggaaactttg
     5521 aaagaactga agaaacaagt ggatgtccta accttgaccg ctacgccaat ccctcgtacc
     5581 ctccatatgt ctatgctggg aatcagagat ttgtctgtta ttgaaactcc gccgactaat
     5641 cgctaccctg ttcagaccta tgttttggaa aagaatgata gtgtcattcg tgatgctgtc
     5701 ttgcgtgaaa tggagcgtgg aggtcaaggt tactatcttt acaacaaagt tgacacaatt
     5761 gttcagaagg tttcagaatt acaggagttg attccagagg cttcgattgg atatgttcat
     5821 ggtcgaatga gtgaagtcca gttggaaaat actctattag actttattga gggacaatac
     5881 gatatcttgg tgacgactac tattattgag acaggggtgg acattccaaa tgctaatact
     5941 ttatttattg aaaatgcgga ccatatgggc ttgtcaacct tatatcagtt aagaggaaga
     6001 gtcggtcgta gtaatcgtat tgcttatgct tatctcatgt atcgtccaga aaaatcaatc
     6061 agtgaagtct ctgaaaagag attagaagcg attaaaggat ttacagaatt gggctctggc
     6121 tttaagattg caatgcgaga tctttcgatt cgtggagcag gaaatctttt aggaaaatcc
     6181 cagtctggtt tcattgattc tgttggtttt gaattgtatt cgcagttatt agaggaagct
     6241 attgctaaac gaaacggtaa tgctaacgct aacacaagaa ccaaagggaa tgctgagttg
     6301 attttgcaaa ttgatgccta tcttcctgat acttatattt ctgatcaacg acataagatt
     6361 gaaatttaca agaaaattcg tcaaattgac aaccgtgtca attatgaaga gttacaagag
     6421 gagttgatag accgttttgg agaataccca gatgtagtag cctatctttt agagattggt
     6481 ttggttaaat catacttgga caaggtcttt gttcaacgtg tggaaagaaa agataataaa
     6541 attacaattc aatttgaaaa agtcactcaa cgactgtttt tagctcaaga ttattttaaa
     6601 gctttatccg taacgaactt aaaagcaggc atcgctgaga ataagggatt aatggagctt
     6661 gtatttgatg tccaaaataa gaaagattat gaaattttag aaggtttgct gatttttgga
     6721 gaaagtttat tagagataaa agagtctaag gaagaaaatt ccatttgata tttttcttct
     6781 ataaaataga taaaaatggt acaataataa attgaggtaa taaggatgag attagataaa
     6841 tatttaaaag tatcgcgaat tatcaagcgt cgtacagtcg caaaggaagt agcagataaa
     6901 ggtagaatca aggttaatgg aatcttggcc aaaagttcaa cggacttgaa agttaatgac
     6961 caagttgaaa ttcgctttgg caataagttg ctgcttgtaa aagtactaga gatgaaagat
     7021 agtacaaaaa aagaagatgc agcaggaatg tatgaaatta tcagtgaaac acgggtagaa
     7081 gaaaatgtct aaaaatattg tacaattgaa taattctttt attcaaaatg aataccaacg
     7141 tcgtcgctac ctgatgaaag aacgacaaaa acggaatcgt tttatgggag gggtattgat
     7201 tttgattatg ctattattta tcttgccaac ttttaattta gcgcagagtt atcagcaatt
     7261 actccaaaga cgtcagcaat tagcagactt gcaaactcag tatcaaactt tgagtgatga
     7321 aaaggataag gagacagcat ttgctaccaa gttgaaagat gaagattatg ctgctaaata
     7381 tacacgagcg aagtactatt attctaagtc gagggaaaaa gtttatacga ttcctgactt
     7441 gcttcaaagg tgataaaatg gaaaatttat tagacgtaat agagcaattt ttgagtttgt
     7501 cagatgaaaa gctggaagaa ttggctgata aaaatcaatt attgcgttta caagaagaaa
     7561 aggaaaggaa gaatgcgtaa attcttaatt attttgttgc taccaagttt tttgaccatt
     7621 tcaaaagtcg ttagcacaga aaaagaagtc gtctatactt cgaaagaaat ttattacctt
     7681 tcacaatctg actttggtat ttattttaga gaaaaattaa gttctcccat ggtttatgga
     7741 gaggttcctg tttatgcgaa tgaagattta gtagtggaat ctgggaaatt gactcccaaa
     7801 acaagttttc aaataaccga gtggcgctta aataaacaag gaattccagt atttaagcta
     7861 tcaaatcatc aatttatagc tgcggacaaa cgatttttat atgatcaatc agaggtaact
     7921 ccaacaataa aaaaagtatg gttagaatct gactttaaac tgtacaatag tccttatgat
     7981 ttaaaagaag tgaaatcatc cttatcagct tattcgcaag tatcaatcga caagaccatg
     8041 tttgtagaag gaagagaatt tctacatatt gatcaggctg gatgggtagc taaagaatca
     8101 acttctgaag aagataatcg gatgagtaaa gttcaagaaa tgttatctga aaaatatcag
     8161 aaagattctt tctctattta tgttaagcaa ctgactactg gaaaagaagc tggtatcaat
     8221 caagatgaaa agatgtatgc agccagcgtt ttgaaactct cttatctcta ttatacgcaa
     8281 gaaaaaataa atgagggtct ttatcagtta gatacgactg taaaatacgt atctgcagtc
     8341 aatgattttc caggttctta taaaccagag ggaagtggta gtcttcctaa aaaagaagat
     8401 aataaagaat attctttaaa ggatttaatt acgaaagtat caaaagaatc tgataatgta
     8461 gctcataatc tattgggata ttacatttca aaccaatctg atgccacatt caaatccaag
     8521 atgtctgcca ttatgggaga tgattgggat ccaaaagaaa aattgatttc ttctaagatg
     8581 gccgggaagt ttatggaagc tatttataat caaaatggat ttgtgctaga gtctttgact
     8641 aaaacagatt ttgatagtca gcgaattgcc aaaggtgttt ctgttaaagt agctcataaa
     8701 attggagatg cggatgaatt taagcatgat acgggtgttg tctatgcaga ttctccattt
     8761 attctttcta ttttcactaa gaattctgat tatgatacga tttctaagat agccaaggat
     8821 gtttatgagg ttctaaaatg agggaaccag attttttaaa tcattttctc aagaagggat
     8881 atttcaaaaa gcatgctaag gcggttctag ctctttctgg tggattagat tctatgtttc
     8941 tatttaaggt attgtctact tatcaaaaag agttagagat tgaattgatt ctggctcatg
     9001 tgaatcataa gcagagaatt gaatcagatt gggaagaaaa ggaattaagg aagttggctg
     9061 ctgaagcaga gcttcctatt tatatcagca atttttcagg agaattttca gaagcgcgtg
     9121 cacgaaattt tcgttatgat ttttttcaag aggttatgaa aaagacaggt gcgacagctt
     9181 tagtcactgc ccaccatgct gatgatcagg tggaaacgat tttgatgcgc ttgattcgag
     9241 gaactcgctt gcgctatcta tcaggaatta aggagaagca agtagtcgga gagatagaaa
     9301 tcattcgtcc cttcttgcat tttcagaaaa aagactttcc atcaattttt cactttgaag
     9361 atacatcaaa tcaggagaat cattattttc gaaatcgtat tcgaaactct tacttaccag
     9421 aattggaaaa agaaaatcct cgatttaggg atgcaatctt aggcattggc aatgaaattt
     9481 tagattatga tttggcaata gctgaattat ctaacaatat taatgtggaa gatttacagc
     9541 agttattttc ttactctgag tctacacaaa gagttttact tcaaacttat ctgaatcgtt
     9601 ttccagattt gaatcttaca aaagctcagt ttgctgaagt tcagcagatt ttaaaatcta
     9661 aaagccagta tcgtcatccg attaaaaatg gctatgaatt gataaaagag taccaacagt
     9721 ttcagatttg taaaatcagt ccgcaggctg atgaagagga agatgaattt gtgttacact
     9781 atcaaaatca ggtagcttat caaggatatt tattttcctt tggacttcca ttagaaggtg
     9841 aatcaattca acaaatacct gtttcacgtg aaacatccat acacattcgt catcgaaaaa
     9901 caggagatgt tttgattcaa aatgggcata gaaaaaaact cagacgttta tttattgatt
     9961 tgaaaatccc tatggaaaag agaaactctg ctcttattat tgagcaattt ggtgaaattg
    10021 tctcaatttt gggaattgcg accaataatt tgagtaaaaa aacgaaaaat gatataatga
    10081 acactgtact ttatatagaa aaaatagata ggtaaaaaat gttagaaaac gatattaaaa
    10141 aagtcctcgt ttcacacgat gaaattacag aagcagctaa aaaactaggt gctcaattaa
    10201 ctaaagatta tgcaggaaaa aatccaatct tagttgggat tttaaaagga tctattcctt
    10261 ttatggctga attggtcaaa catattgata cacatattga aatggacttc atgatggttt
    10321 ctagctacca tggtggaaca gcaagtagtg gtgttatcaa tattaaacaa gatgtgactc
    10381 aagatatcaa aggaagacat gttctatttg tagaagatat cattgataca ggtcaaactt
    10441 tgaagaattt gcgagatatg tttaaagcaa gagaagcagc ttctgttaaa attgcaacct
    10501 tgttggataa accagaagga cgtgttgtag aaattgaggc agactatacc tgctttacta
    10561 tcccaaatga gtttgtagta ggttatggtt tagactacaa agaaaattat cgtaatcttc
    10621 cttatattgg agtattgaaa gaggaagtgt attcaaatta gaaagaataa tctttaatga
    10681 aaaaacaaaa taatggttta attaaaaatc cttttctatg gttattattt atctttttcc
    10741 ttgtgacagg attccagtat ttctattctg ggaataactc aggaggaagt cagcaaatca
    10801 actatactga gttggtacaa gaaattaccg atggtaatgt aaaagaatta acttaccaac
    10861 caaatggtag tgttatcgaa gtttctggtg tctataaaaa tcctaaaaca agtaaagaag
    10921 gaacaggtat tcagtttttc acgccatctg ttactaaggt agagaaattt accagcacta
    10981 ttcttcctgc agatactacc gtatcagaat tgcaaaaact tgctactgac cataaagcag
    11041 aagtaactgt taagcatgaa agttcaagtg gtatatggat taatctactc gtatccattg
    11101 tgccatttgg aattctattc ttcttcctat tctctatgat gggaaatatg ggaggaggca
    11161 atggccgtaa tccaatgagt tttggacgta gtaaggctaa agcagcaaat aaagaagata
    11221 ttaaagtaag attttcagat gttgctggag ctgaagaaga aaaacaagaa ctagttgaag
    11281 ttgttgagtt cttaaaagat ccaaaacgat tcacaaaact tggagcccgt attccagcag
    11341 gtgttctttt ggagggacct ccggggacag gtaaaacttt gcttgctaag gcagtcgctg
    11401 gagaagcagg tgttccattc tttagtatct caggttctga ctttgtagaa atgtttgtcg
    11461 gagttggagc tagtcgtgtt cgctctcttt ttgaggatgc caaaaaagca gcaccagcta
    11521 tcatctttat cgatgaaatt gatgctgttg gacgtcaacg tggagtcggt ctcggcggag
    11581 gtaatgacga acgtgaacaa accttgaacc aacttttgat tgagatggat ggttttgagg
    11641 gaaatgaagg gattatcgtc atcgctgcga caaaccgttc agatgtactt gaccctgccc
    11701 ttttgcgtcc aggacgtttt gatagaaaag tattggttgg tcgtcctgat gttaaaggtc
    11761 gtgaagcaat cttgaaagtt cacgctaaga ataagccttt agcagaagat gttgatttga
    11821 aattagtggc tcaacaaact ccaggctttg ttggtgctga tttagagaat gtcttgaatg
    11881 aagcagcttt agttgctgct cgtcgcaata aatcgataat tgatgcttca gatattgatg
    11941 aagcagaaga tagagttatt gctggacctt ctaagaaaga taagacagtt tcacaaaaag
    12001 aacgagaatt ggttgcttac catgaggcag gacataccat tgttggtcta gtcttgtcga
    12061 atgctcgcgt tgtccataag gttacaattg taccacgcgg ccgtgcaggc ggatacatga
    12121 ttgcacttcc taaagaggat caaatgcttc tatctaaaga agatatgaaa gagcaattgg
    12181 ctggcttaat gggtggacgt gtagctgaag aaattatctt taatgtccaa accacaggag
    12241 cttcaaacga ctttgaacaa gcgacacaaa tggcacgtgc aatggttaca gagtacggta
    12301 tgagtgaaaa acttggccca gtacaatatg aaggaaacca tgctatgctt ggtgcacaga
    12361 gtcctcaaaa atcaatttca gaacaaacag cttatgaaat tgatgaagag gttcgttcat
    12421 tattaaatga ggcacgaaat aaagctgctg aaattattca gtcaaatcgt gaaactcaca
    12481 agttaattgc agaagcatta ttgaaatacg aaacattgga tagtacacaa attaaagctc
    12541 tttacgaaac aggaaagatg cctgaagcag tagaagagga atctcatgca ctatcctatg
    12601 atgaagtaaa gtcaaaaatg aatgacgaaa aataaccctg agagaggctg gagcctctct
    12661 tttttgtgca gtttaggagc taaagggaac agaatggaga aaatggaaca aatgtgtttt
    12721 ctaatctgtt agactgtatc tagaaagggg aaaattatga ttaaagaatt gtatgaagaa
    12781 gtccaaggga ctgtgtataa gtgtagaaat gaatattacc ttcatttatg ggaattgtcg
    12841 gattgggacc aagaaggcat gctctgctta catgaattga ttagtagaga agaaggactg
    12901 gtagacgata ttccacgttt aaggaaatat ttcaaaacca agtttcgaaa tcgaatttta
    12961 gactatatcc gtaagcagga aagtcagaag cgta
//
LOCUS       DAVKJD010000051        12529 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKJD010000051 DAVKJD010000000
VERSION     DAVKJD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.051"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..324
                     /locus_tag="VW157_001455"
     CDS             <1..324
                     /locus_tag="VW157_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9552737.1"
                     /translation="CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSK
                     LKPLTAEDKACNHALSKERSKVENIFAKVETFKMFSTTYRNHRKRFGLRMNLIAGIIN
                     HELGF"
     gene            complement(394..720)
                     /locus_tag="VW157_001456"
     CDS             complement(394..720)
                     /locus_tag="VW157_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552738.1"
                     /translation="MILWSFDFANDHAHAFFMDNVEWSHADSYFLSFVSDDVEERYTE
                     NVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSVGTSPEQYPDYDG
                     FDYEEW"
     gene            complement(1134..3014)
                     /locus_tag="VW157_001457"
     CDS             complement(1134..3014)
                     /locus_tag="VW157_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9552739.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRHDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3008..3877)
                     /locus_tag="VW157_001458"
     CDS             complement(3008..3877)
                     /locus_tag="VW157_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9552740.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSIPTPESLEDLLSWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQIPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGQTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3960..6605)
                     /locus_tag="VW157_001459"
     CDS             complement(3960..6605)
                     /locus_tag="VW157_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9552741.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDMATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(6696..7976)
                     /locus_tag="VW157_001460"
     CDS             complement(6696..7976)
                     /locus_tag="VW157_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9552742.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTIWSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8147..10231
                     /locus_tag="VW157_001461"
     CDS             8147..10231
                     /locus_tag="VW157_001461"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9552743.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNPLMLFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDVAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(10271..11950)
                     /locus_tag="VW157_001462"
     CDS             complement(10271..11950)
                     /locus_tag="VW157_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9552744.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGSNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQALPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3419 a         3004 c         2556 g         3550 t
ORIGIN      
        1 tgtcatgata tgaagttgtt caaaatgagt cgcagaaata ttggacaagc tggtaaaatc
       61 ttggctgaca gtggttatca agggctcatg aagatatatc ctcaagcaca aactccacgt
      121 aaatccagca aactcaagcc gctaacagct gaagataaag cctgtaacca tgcgctatcc
      181 aaggagagaa gcaaggttga gaacatcttt gccaaagtag aaacatttaa aatgttttca
      241 acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgat tgctggtatt
      301 atcaatcatg aactaggatt ctagttttgc aggaagtcta ttgaaatact aagcctacac
      361 agactgattc caattgtttt ttagatttca attttaccat tcttcatagt caaaaccatc
      421 ataatctgga tattgttctg gcgacgttcc aaccgaacgt acgagataag cttcttcgtc
      481 ctctgtctcg atttctctca gcacttggca ttcaaaacgc cattcatcac cgaagtcgaa
      541 aataaactta aatttttgtt tgacacttag gctatccaga tagacatttt ctgtgtaacg
      601 ttcttcaaca tcgtcactaa caaagctaag aaagtaagaa tctgcatgac tccactcaac
      661 attatccatg aaaaatgcgt gagcatgatc atttgcaaaa tcaaaactcc ataaaatcac
      721 atctgctagt tccgctaacg tttttgtttt tggaatatat atatgtcggt agcaaccttt
      781 tttaaatttg acggaaatga tccaagcttc tacagatgct ggcttttcag cttctatttc
      841 ttgttttact aattttaagt gtagcataat acctccaatt gctcatcgat agaattgctc
      901 ttaggaaaca ttcactttct gctattttat cataagtttg tctaactgta gcatttgaaa
      961 ccagataatt cttctgtttc acgcgaaaca ccctgcgcca agcaaggtgt tcttgttttt
     1021 atactcaatg aaaatcaaag agcaaactag gaagctagct gcaggctgct caaaacactg
     1081 ttttgaggtt gtagatggaa gctgacgtgg tttgaagaga ttttcgaaga gtattaagtc
     1141 gtataaatcg tcgacgctgt cgcaatggta tgccactgat tagctgtagt tgctgcgaag
     1201 tccttgtctg cgtagaagtc agtaaatggt aggatttcaa cttccagctc gtcgatgcgg
     1261 tcaagctgac cagccagata aacctcgata cggctttctg ctcgtttgat gcgttgcaag
     1321 agtccgccca tacggatgtc aactgtatcc aaaccaaaga ccttgttttc tttcagccat
     1381 tggtggctaa agagggcatg gaagtcttca atttggcttc taagttctgg taattcttgt
     1441 ctggcgattt gttgtaaact ttctttatca tccgcttggt aggcctgacg aatgcgtcgt
     1501 cccacatcta ctttgctact taaaatagca ttcaactggg cctgagtttc aaagagataa
     1561 gcatagtttc cagctttttc tttaatgtta gcaagcgtct cagcagcctg agcgaagtgc
     1621 ggtttgtcct gttcaggtgt catgtgtcgg tcaagtatcg gacagagaac atcctgataa
     1681 aagacatatc ggttgggatt gatgccactg agattgcctg gtaggtctgg taagaggttg
     1741 gcaaggtcaa tctgcataaa atcctcaacc gttagaccag tattggtctt gaagtgcgca
     1801 gacaaactat ctaggtcatg gcgatagctg agttctgccc agatttgcaa gcttggtagg
     1861 atagagaact gggcagtttc acccccattg tctccccaac ccgttacgat gacttcttta
     1921 atctgattgg cacggcaggc tttattagcc tcgatagcca ctagacggct aaaatggttg
     1981 tgaggtgtaa agccaatcca cttccaagct ccccctgcaa atgcaaggtc atggctaatc
     2041 ttgtgatgat tgcggaaatt acggttgtat ttttcctcgc tatcctgata ataatcccag
     2101 taaaccagag tcacacggtc tttgagacgg tctaggtaga cacgagtttc ctctggaatt
     2161 tccacatcac ggtcgtactg gccatccgct gacatgagtt tgaagaacat atcactccac
     2221 atctggcagt ggaaaccata tttgtcagca atatccagca cgcgctccaa gtgttggcac
     2281 atgaggagac tacgatccac aacaccgttc aaaatcaggt agcgtcccaa accaaccaag
     2341 tgggcttcgt ccatcccgat attgaccttg cgagtcttca gtttagacaa cgtggcaaac
     2401 atgccatcaa tcaggtcata aaccttttct tcgccaatga gaagaatgtc ctctacatca
     2461 cggagctcct gcacttcctt gacaccccat ttgacaaagg ccgacaagtg ggccaaggtc
     2521 tggatgcatg gtacaaaggt catgtcaaac tgttgagcat aggcttcgat ttcctgcaac
     2581 tcctctgctg aataagctcc acggaaatag ccaaagtaag gctgtccttc aatctggtaa
     2641 gtgtcttcca tgtaaagctc aaaggttgag tagcccatga gagccaagac ctcaatcatc
     2701 tgcttggcag aagccacatt cagcaccgca tttcgagaac agtcaaccat gtaagccaaa
     2761 tcttcgtaag ctgcttgttc ctcaatctct actttatcag cttctgctag aactgttacc
     2821 aacaaggata aggcacgata gagttggtga ggtttgcggt aggtcaattg atagtgacct
     2881 tcctcaccct ggatagagat agatgcttgg tcagactgag tgacagccac ttccacatct
     2941 ggtagagaaa tatgaccttt taaaacctca atagcttgcg tttgtttggg actaagtcct
     3001 gtaaatctta ccattgcttt tcctcctgta gccagttaac aagagcaccg tagagattgg
     3061 catctgcgtg ataggtgcag gcctggataa ctggggcgac cgtgtattct tcgtaggcat
     3121 cgacaaagtc ttcaacagcc ttcttgacac cttggataaa atctggattt tgactgatag
     3181 agccacccag actgatgaca tctggatcga tgagatactg gatattgagc aagccttgcg
     3241 ccagattgcg gttcatgcgc tcaatggctt cttgacaaag agcattacca gctgcggcct
     3301 cttggtaaat cttgcgaccg tcccaatcag tctgaccaga tttttcaatc acgtagcgca
     3361 ccatattccc agttgacgct agttgcgacc agttgttgag cttttcagca ggagcaaggg
     3421 tggtcatgta gccaaattct ccacccaggc cgtggcgacc tcgatgaagt ctaccattga
     3481 taatcatggc tccgccaatc cctgtcccaa tcacgacaca ggctgcattt tcaagttctg
     3541 gatgagctag caattcactg agtccaacgc agttggcatc attttccaga tggacaggaa
     3601 tctgataaga gctaagcgcc tcataccaag aaaatccgtg aatgtagggc accgcactga
     3661 agccatcaat cacacccgtc tcttgattga ccgcgcctgg aacgctcata gcaatcccgc
     3721 tgtaatcctg ctctgacaag cgctggtcta accaagatag taaatcctcc aaactttcag
     3781 gcgttggaat gcttgtctta tccaatattt tcccatcagg agtcagactg gcaaacttaa
     3841 tcccagtccc tccgatatca atcgttgcaa tggtcatttt tttaccataa aaaggggcaa
     3901 atcagcagtt agttcttact ttttcgcccc tcctttcttt tgatgtttac tttttgaaat
     3961 taaatttctt cttttttgat gaattctgta cgaatctctt gtggagccaa tagtcctgaa
     4021 tgaactggat atggtcgctc aagtaagtca agaatggttt gttgatgttc tgatatgcgc
     4081 acattttctt gactcatatt atagtaacgg aggacatagc cttcttcatt ttccgccact
     4141 ttaaaggctg ttggacaaac ttgcggtatg ctgagaacag aatggctcaa gagactacca
     4201 gtcgcaacca cgcttccttc ctgtctagca agctgaaggc tggtaaacgg tgtctgcaag
     4261 gctttggcac gacgataggc tgagaagcgt tcttgggctt ggtggcattc aagtgcaaac
     4321 tcgacttcaa actcacgcaa gcattgtgct tctggcgttg ggaagtagcc ccagtcacct
     4381 agctcacctg atgcacgcaa aatggtcacg gcaatggtgt tatccccaag gatttcgtat
     4441 tcattcaatc ccttattgga tacagtcacc cctttttcat cgtcatacag actgacaaaa
     4501 gcttgttggt gttgaggatt ttcagggttt tcccatgaag cagctggttt gtttggtcgt
     4561 gtcaccacct catagatact ttcagaatca ttgcttggac gcgtgttatg agtcttgacc
     4621 aagagacgga tacggtgatc cttggcagtg ttagtaaagc gagtcttgaa gcggatttgt
     4681 ggattgtcaa cgaagacagt caactcagtt tccagaggaa tgtttgtcaa ttcttctgac
     4741 cgtccagcct cacgcttcat aaactcgatg ataccttgtt gctcttcttc tagcttttca
     4801 tccgcactga caggcacggt caattcatgt ttgagcaaga ttttagcata gcaagctgtg
     4861 ttttccaaga cctcgtggcc cttaagctct gcaaagattg gctctgttcc ttttggttgg
     4921 aaatagatat actcgtttcc gatgtcccca cggtcttcaa agcggataaa gtcttcatag
     4981 gcttcgtgag ttgtcttgtc atagactgtg atgttgtcat ccacactcac cgttacgaat
     5041 ggtgtatcaa tcactccgtt ttggtaaata ccctcacggt gttcttgttt tccttccagc
     5101 aattggaagg ttgtccaaga aagcggtgct aggtgaactg gaatggtcac gcgcacttgt
     5161 cgagcaatac gagcttggcg gaacttatct tttggtaaat cgtattcaaa attagctccg
     5221 aggtcttcga ttgtagcctc tacaggacga ccatccaagt cctccacacg gtaacttggc
     5281 aagataagag cagccatctt tttgtagcct tctgttgggt gcaattcctt gaaatcacaa
     5341 gtcgccatat caatcactgt gctgacagta tcgaccttat catgcaagcc tgtgttaatg
     5401 acagtaaaga gatagtcact ttgagcctta tccgtagcaa ttttaccctt ccactcgttg
     5461 agcaaattac ttttaacaaa gtttcctact tggttgacct tggcaaaacg cgtttccatc
     5521 tcgcggtgaa cttcgtccac gctacagcca cagatactat catgtggcgc attctgcaaa
     5581 agtgttttcc aagcataggt caactggtcc ttgtggttgt gtccaccagt gataatagtc
     5641 aagggttcta ccacttgctc taggaggttg ctattttctt ggaaggcttg ttttaggtaa
     5701 atgcgggatg aagaagtgtt ggcaagtgtg taccagccat ctgtttcctg actggtcaac
     5761 tcacctgtaa cagttgataa gtgttcagga agcgcacctt ctacagcttg aacatattca
     5821 tcaaaagaac tatgaacaaa gattacatcc gggaagagtt catttgccac acgaatggct
     5881 tcgctcagat ttttctggac aggctggtgg tcacagccgt tcatcatcaa ccattggttg
     5941 gtcgaagcgt aggcacgcac atctgacaat ttttgtttcc agaaggtcaa ggcctcatct
     6001 ttgtcaactg ggatttcatt cccattactg taccagttgg caaagagaat acctaaaaca
     6061 cgactaccat ccacaccctg ccagtacatt tctgaaaact gggatgtaaa ctgctcatct
     6121 tcaaggactt ggttgtcaaa tccaatcggc ttcacaccac gaccaaaggc cgccacgtga
     6181 atgcctgatt tttgaagaat ttgaggcgct tgtcccatat ttccaaaggt atctggaaag
     6241 tagccaatct gggttgattt accccatttg gcagcttctt gttgaccaat caaggtattg
     6301 cggacattgg cttcactgga gatcaagtag tcatcctgca agatgtaaaa gggaccaatt
     6361 ttaagtttgc cctcgtcaat gtagcgttgg accttgtcgc gattttcagg gcgaatttgt
     6421 aagtagtcat caaggacaat agtttgtcca tccaagtgga aactcttgaa ctcagggtca
     6481 ttttcaaaga gatcaaagag attgtcaaac aattccacca actgcatacg atggctttca
     6541 aaaggcaagt accactcacg gtcccagtga ctatgtgaga taatatgtac aacaacattt
     6601 tccatgaagt aaaacctcat tctaaattta aattttaacg ttttaaatgt aaacttgtga
     6661 ttctgataag aatcggttga gctaaagcga gccccttagc ggatatccaa gtaatccaag
     6721 accaactcac agaacatcat gttagcccag gagaaccatt cacgagagta gagggtcgga
     6781 tcatctacgt ggaagctttc gtgcatgaca cctgtaccac catcgcaggc aaccagctga
     6841 tccagcaaga attttttctc tgccttatct cttgttgtca atccttggat agaaagggcg
     6901 attggccaga tatagcgata gaaggtatga gaacttccga gaccgctagc gtattctcct
     6961 tggtagaagt atggattttc agagctccaa atggtacgac gagtagcttg atacacttca
     7021 tcatcgaccg aacagtagcc cagatagggc gcagctagta gacttggtac atttggatca
     7081 tccatgatgc tggcatttcc taggccatcc acttcaaaag cgtaaatctt ttcgcccttg
     7141 ctgttggtgg tgtaagcgta gttttcgatt ccttcttgga tttcatcctg aagacgcttg
     7201 gcatcagcaa taacactctg gctatcagct aggtttaatg ctgcgaagat ttcttgcaca
     7261 taacccaaga ctactacagc aaacatattt gacggaatca agtaactata ctggcaacag
     7321 tcatcactcg gacgaaaggc tgaccaagtc atacctgtca ctgcaaagtc aggtccaaag
     7381 ccatcattta ccaaggtgtc ttccttacgg tccgtatctc ggacaaaacg ataaggagag
     7441 ttcttgtggt cttgttcaac tgtccataga tggagaattt ccttagtcgc tgcgacaaaa
     7501 gtttcatcaa actgactagt ctcgccagtc tctttccaga ggagataagc caactgcaaa
     7561 ggatagcaaa gcgaatccac ctcatacttg cgctcccaga tccagccgtt aaggtctgtg
     7621 tggtcagtct cgtggtgccc tttccagttc tcctcaatgt tgaaggagtt agcataggga
     7681 tccttgagta ccaaggtcat ctgacgtttg accaaacctg caatggtctg acgcaggagg
     7741 gcatctcttt tagctacatg aaggtagggt ctgagttggg ctgtcgaatc tcgaagccac
     7801 atggcaggaa tatccccagt caagacaaaa gttgaaccat cttctaagat ttcaaccgta
     7861 ttgtccaagg tatcggtgta gcaacgctcg aaaacatcca cccactctgg atggtcctta
     7921 gcccgctctg ctacttcatc tagccattct ctaacaattt ctttcgaata aatcatcgtg
     7981 caatttcctc tttcaaacaa gtttcatttt cagtatatag cttttgagac agaaaataca
     8041 tacacaaatg atagtcattt ttctacaaaa tagttatttt tgaaactcct tttctaaagg
     8101 ccctcttttt ctgatataat gtagaaaaac agctaaagga aagactatga aaccactact
     8161 tgaaaccatc gatacccgct ttggtactgc tagcaagcat gccttctctc ggggaaatac
     8221 cctgccatac acaggcgtgc cttttgggat gaattatttt gtgccccaga ccagtgacca
     8281 ggatggatct tggttttttg atccgcatct gcctatcttt caggggattc gattaactca
     8341 ccagcccagt ccttggattg gggactactc ttggctcctt ctgacacctg tcaccagtca
     8401 actgggggga gacagcctct ttcaccgcca gtcttcttat gatatagata aagcttgctt
     8461 ccagcctcac tatttgaagc tcttttccct gcgctatcaa attgaaaccc agctcacacc
     8521 gacttgctat ggtgcttcta ttcgtttgaa gcaaaagcaa ggcaaagccc tctccctcta
     8581 tcttcacgca gcagatgaac taacagttga gcaggtagat aagcggactc tggctctcag
     8641 acaagaaggg aaaacagaga ccaataaaaa tccactgatg ctcttcactg ccctgcaaat
     8701 gaacacggat attcttgcta tcagccaaga agctggagac tggcgaattg acttagcaag
     8761 tagtcaaacc gagatgcaac tagccacttc tttcatctct ccttctcaag ctctaatcaa
     8821 tctacctcaa gaagattttg atagctgtaa aacaagtgcc caagcggatt gggaaaatct
     8881 cctccatcgt tttgacatta tagagacagg agaggctgac cgaaccttct ttgaccactg
     8941 cctctacaga ctcttcctct tcccacagac tttttatgag gttgatgagt cagggcaagc
     9001 catccacatg gatctggcta ctggtactgt caagcctggt gtcctcttta gcaacaatgg
     9061 tttctgggat accttccgca ccaccttccc cctctttgcc cttatcatac cggagcacta
     9121 tcaacgcttt ttagaaggtt tcctcaatag ctaccgcgat actggtttcc ttccaaaatg
     9181 gctggctcca gatgaacggg gtatgatgcc aggtacactt ttagacggta ttatcgcaga
     9241 tagcgcctgc aaggacgtgg cccctgacct agaaggagaa ctcttccaag ccatgctcga
     9301 aacagccagc aaagccgacc ctctcggaat caatggccgc cacggactag cccaatacca
     9361 agaactaggt tacctctcta ccgaccacca cgaaagtgtt agccatactc tagactatgc
     9421 ctatagtgac ttttgtatcg ctagctgtgc caaaaaacta gagaacatag aaatcgctga
     9481 aacctacaag gctgcttcac aaaattaccg ccagctattt gacgctgaga caggttacat
     9541 gcgagcacga gacaatcaag gaaactttca ccctgacttc tctccttata gttgggggcg
     9601 agactacgct gaatgctctg ccattcaagc tactttaggc gttctccacg acatccctgg
     9661 cttaatccaa ctgatgggtg gaaaagaaac ctttagcaac tatcttttga aagcttgtca
     9721 agatgctccc ctctttgaaa caacaggcta tggttacgaa attcacgaaa tgagcgagat
     9781 ggctactgct ccttttggac aaatagccat ttccaaccag cctagtttcc acattcctta
     9841 tctcttccgc tacagcgatt accctgacta cactgccctt ctcatcaaga cgctccgtca
     9901 gaaagctttt cacccaagtt gggaagccta tcctggagat gaagacaatg gtagtctctc
     9961 tgcttggtac atctggtcag ctctcggatt ttatccaacc tgtccaggaa aaccaagcta
    10021 tgacctcgga attcctctct ttgaccatct acgagtctac ctagctaaag aagataaatg
    10081 gctggatatc catactaaac aaaaccacaa ccattttaac tttgtcaaag aatgccgact
    10141 ggacaaaaca ctcgtatcaa ctattcaaca ccaagacctc ttaaaagctg aacaactaac
    10201 tttcaccctc agctggttac caagtcacta ataaccaaaa gaacctttgc atcgcgcaaa
    10261 ggttcttctt ttatgaaact tggacttgaa gttgatccac aagctgacca tccaccgtta
    10321 ggttcagata gaaatccaag gttttatctc ctgcgaaata cagggttggt agatgcaaat
    10381 cttttttgct ttcccctgct tggaattgaa ggacttgaat ctcatcctga taggcgacac
    10441 catggacacc tgtccccggt tgaatcgaaa tcttagcctc taaagaggag ctactttcac
    10501 tacgttcaat cctaaaatga agagtctctc cctttgccac agctaggctt ttttctgcaa
    10561 atgctagccc ctgaacagct actgtttgtg ataacctcgg agttttatag agtgaaatct
    10621 tggtcaacaa aggcaaagcc tgtgattctg taatgactac acgtatcttc tgtgcttcaa
    10681 caactgctcc tcgtaagaga cgtttgtaac caacagtatg acccgaacca aactcctgcc
    10741 agacaccatc cacctctact tgcacatgaa aagcagcgat tcgttgccct agcttcaaat
    10801 cttctcttaa ctcaattaca tcaaaagttt taggagaacc taagtcgagt tctaactgga
    10861 tgggcaagtc tgcatcgttt gcccaagagc tggtctcaag gccgtctgtc aaatggcgac
    10921 aagcaaagtc tgcggaaaga gctggaccag atacctcagc tcccagagcc aaatcttctt
    10981 tatagagctc attgcgatag gtcgcaaatt cataaagtcg ttcaatatcc tttgcatcaa
    11041 agagcccagc ttgattcggc ggaatattaa gcaagagtgg agttcctcgc cctactgagt
    11101 gaaagtagat ttcgaccaac tcctcgagag acttaggatc ctgatcctca tggtagaacc
    11161 agcctggacg gatggaaaca tctgcctctc cgattgaaaa aatcgtgccc gagggatccc
    11221 cgtgctgaag atagttcagc tctgcttctg ttcctagttt atcaggattc accttttgcc
    11281 acagtggatc acctgcatac cctcgttcat tgccaatcca gcggatactg gtgccttctg
    11341 ttgaaaaaat caagcaagcg ccctgcaggt cacgaatggt ttcaaaccat ttttcaaatt
    11401 cataattaac cttttgcgcg ccctctcctc tggcaccatc catccaaacc tcagcgaact
    11461 taccagcatt cccatagtta ggatttgata agatttcctt caactgagcc agataatagg
    11521 cattgtagtc cgcttctcgg tccacatgat agaggggact atgggcatcc cacggtgaca
    11581 ggtagacccc catatccata tcaaactctg tggcagcttg ggatacttca aggagcaagt
    11641 cgccctttcc tctcctccaa ggactgacct taaccgaata atctgtgtga gctgtcggat
    11701 aaaggacaaa gccatcgtgg tgcttgacca ccaaaatcaa ctttttgaag cccgtttcct
    11761 tgagcacacg aacccactca cgcgcatcca actgactcgg gttaaagcgc tcaggatcct
    11821 cctgtccagt cccccattct tggtcataaa aggtattaga accgaagtgg ataaaagctg
    11881 ctagttcatc tcccagataa gctagctgag tctgacttgg taacggtcca tgcggtttga
    11941 ttttcttcat cgttatatat ctccttcttt agcaacttct cttgttcaca aggttatcca
    12001 caggttgtgg ataataaacc atgatctaac agccagctct ttccaactcc aactggtagg
    12061 ctcaggattt ttctcctcaa tattccctct tcaaacagct ctgtcatatg gttctgctaa
    12121 cacctctcag aatgctactc tccataatta ttctcaggct tctttcaggt cattgtgtga
    12181 agaccattca ctctccagac ttttccacag attgtgaata agtgattatt ctaaactatc
    12241 acctcgtcga ctataacaaa aaataaaaag agggtcaata agataattat ctcttcctaa
    12301 atactcagaa aagattctat aatttgtaca attgaacaag aaatgcttct ccaaaatcta
    12361 tcttcaaaag aatgttgcat ctttttatcc tttcacacaa acatttctct ttatacagat
    12421 ccaagtgata accagatgat aattggacac tttttggtca tttgtatatt ttttgaaaaa
    12481 ttttttaaaa aaattttata tcctttttat agaagtttat gcatttgta
//
LOCUS       DAVKJD010000052        12466 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKJD010000052 DAVKJD010000000
VERSION     DAVKJD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12466
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.052"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..536
                     /locus_tag="VW157_001463"
     CDS             <1..536
                     /locus_tag="VW157_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9552745.1"
                     /translation="HLSRAMSRVRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSDK
                     RFYRPTFRMHLTNKEILNKLLSYSEDLKHHYQLLLFHFQNKEPEKFFGLIEDNLKQVH
                     PIFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIF
                     IALNIKKERTKFVLSRA"
     gene            complement(798..1772)
                     /gene="pta"
                     /locus_tag="VW157_001464"
     CDS             complement(798..1772)
                     /gene="pta"
                     /locus_tag="VW157_001464"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9552746.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(1816..2712)
                     /locus_tag="VW157_001465"
     CDS             complement(1816..2712)
                     /locus_tag="VW157_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9552747.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(2709..3527)
                     /locus_tag="VW157_001466"
     CDS             complement(2709..3527)
                     /locus_tag="VW157_001466"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9552748.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(3511..4182)
                     /locus_tag="VW157_001467"
     CDS             complement(3511..4182)
                     /locus_tag="VW157_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9552749.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            4310..4879
                     /locus_tag="VW157_001468"
     CDS             4310..4879
                     /locus_tag="VW157_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9552750.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLNKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            4992..5951
                     /locus_tag="VW157_001469"
     CDS             4992..5951
                     /locus_tag="VW157_001469"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9552751.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            5961..7076
                     /locus_tag="VW157_001470"
     CDS             5961..7076
                     /locus_tag="VW157_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9552752.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            7081..7428
                     /locus_tag="VW157_001471"
     CDS             7081..7428
                     /locus_tag="VW157_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9552753.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGTKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            7436..7651
                     /locus_tag="VW157_001472"
     CDS             7436..7651
                     /locus_tag="VW157_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9552754.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            7734..8375
                     /locus_tag="VW157_001473"
     CDS             7734..8375
                     /locus_tag="VW157_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9552755.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            8393..9082
                     /locus_tag="VW157_001474"
     CDS             8393..9082
                     /locus_tag="VW157_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9552756.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(9079..9759)
                     /gene="radC"
                     /locus_tag="VW157_001475"
     CDS             complement(9079..9759)
                     /gene="radC"
                     /locus_tag="VW157_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9552757.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQVSV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(9802..12093)
                     /gene="pcrA"
                     /locus_tag="VW157_001476"
     CDS             complement(9802..12093)
                     /gene="pcrA"
                     /locus_tag="VW157_001476"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9552758.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKRNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            complement(12284..>12466)
                     /locus_tag="VW157_001477"
     CDS             complement(12284..>12466)
                     /locus_tag="VW157_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9552759.1"
                     /translation="LIAEDKAYNHALSKERSKVENIFAKVKTFKMFSTTYRSHRKRFG
                     LRMNLSAGIINHELGF"
BASE COUNT         3770 a         2686 c         2371 g         3639 t
ORIGIN      
        1 aacatcttag ccgtgctatg agtcgtgtgc gtgtccaaat catgaatcag tttcatcgaa
       61 aatcccatga atacaaggct atcaagcgct actggaaact cattcaacag gatagccgta
      121 aactgagtga taaacgtttt tatcgtccta cttttcgtat gcacttaacc aataaagaga
      181 ttttaaacaa gcttttgagt tattcggaag acttgaaaca ccactatcag ctcttgcttt
      241 ttcacttcca gaataaggaa ccagagaaat ttttcggact tattgaggac aatcttaagc
      301 aggttcatcc tatttttcag actgtcttta aaaccttcct caaagataaa gaaaagattg
      361 tcaacgccct tcaactacac tattctaatg ccaaactgga agcgaccaat aatctcatca
      421 aacttatcaa gcgcaatgcc tttggttttc ggaactttga aaacttcaaa aaacgaattt
      481 tcatcgctct gaacatcaaa aaagaaagga cgaaatttgt cctttctcga gcttagcttt
      541 tcttcaaccc actacagttg acaaagagcc aaaaaattct cttttcatac tcatttaaaa
      601 tcaaaaaggg aatttatcgt tttacggaca tgtatattac agatttctat aggtcgtgta
      661 tagacaggat atcatcgctt ggaagttttc ctttttgaca ccaatcagta atttagttct
      721 caggaattaa tggcagtgct tttcgtacat agtttcataa ctacagtata gcatagtata
      781 tcactttata gttttcacta ttgatgaact gcttgagctg ctgtgatgag ggtcaacttg
      841 taaacatcat ctgcattaca tccacgagaa agatcattaa ctggcttgtt taaaccttgc
      901 aaaacaggtc ctacagccgc aaagccaccc aggcgttcag ccatcttgta accgatattt
      961 cctgcctcga tacctgggaa gatgaagaca tttgcttgac cagctactgt acttcccgga
     1021 gctttcagag ctgcagtttc gggaacaaag gccgcatcaa attgcaactc accatcgatt
     1081 tcaaggtcag gacgcaagtc gtgagcaatt ttagttgctt caacgacctt atcaacgctt
     1141 tcaccaaacc ctgaaccttt agtagaatag ctcaacatgg caattttagg ttcgatgcca
     1201 aacatcttag ctgtgattgc tgagttgatg gcaatttcag ccaaggcttc tgcatctgga
     1261 ttgatattaa tggcacagtc tccaaatagg taacgttccg taccacgaac catgaggaag
     1321 gctcctgaag tacgagttac atttggacga gttttgatga tttgtagagc tgggcgaact
     1381 gttgaagctg ttgagtgaat cgctcctgac accattccat caaccaagcc caagtaaacc
     1441 aacatcacac caaagtagtt gacatcttca accaaaacct tgcgtacatc ttcttcagtc
     1501 attttgccct tgcgacgctc caccaaggca gaaaccattt cttcaaattg aggataatgt
     1561 tgagggtcga tgacctcata accatccatg attccttcaa tttcaagata aattttaatt
     1621 ttttcaggat ttccaagcaa aacaggaatc acttctgttt cttttactaa gcgttttgtt
     1681 gcttgaagaa tacgaggctc ttccccttca gggagaacga tacgagcatt tttaccaaca
     1741 aggttggctt tgagactttc aaaaacttcc atgagttttc tcctttaaga taataattat
     1801 actctgagac agtttttata gagtattagt tgataactgg gtaataaggt tactgaaatc
     1861 atccggcaag ggactttcta actgcaagtc ttgctctaaa aatggatgat aaaaggatag
     1921 gtaatggcaa tgcagagcct gacgttgaat accatcttcc agactaccac catacaaatc
     1981 atctcccagc aaaggaaaac cgatatgaga aaaatggact cggatttgat gggttcgacc
     2041 agtgtgcagg tgaatataga ccaagtgaat atttccataa gaagctacaa tcttgtatga
     2101 agtatgggca tactttccgc ctttagccac tcgtctggta ataatggaat cttcatcacg
     2161 cgcaatcgga gcaataattt ccccttctgg ctccaaatgt ccatctccct taaccaaagc
     2221 aaagtagcgt ttctcgatag atttcttctg caactgcttg tctaatcgtg catgggcata
     2281 accgtgcttg gcaaagagca tcaagccaga agtgtcccta tctagtctgg taacaatgtg
     2341 aacctgctga ttttcataat tttgcttgac atagtaaccc ttgataaaat tggcaatggt
     2401 attagagtga ttgacactag gaatagaagc cactccatag ggtttattca agactagaaa
     2461 gtggtcatcc tcatagagaa tatctaatgg aagctcaata gcctccaagg tttcaaagcc
     2521 tttctcagcg ggaatgtcaa tggtaacgta gtctccaacg tccaatagat acgttgcatt
     2581 ttgcggttga ttattgacca gaatagctcc acctcgaaac ttaatcttgg ccagcaatcc
     2641 cttagaaacc tcgtgctttt ttaagaaggt cttaaccttg acatgttcat ctgcgataaa
     2701 ttcaaacctc attcatccac ctcaccgata aaggcatcct taacacggtt ccagaaactg
     2761 gtatggctag gagtcgcgac aaagtgaatc ttatgatggt cgatttgata ctcaatacgc
     2821 tcaatattac ggaaagaata aacgctattg tcaaccgaaa tagtatgata atcgtttctt
     2881 gttggaataa gttcaatctt atccttctta ggcacaataa tggaagagcc cagtgttcga
     2941 tagacacgat tattaaggct ggcaatttcc gttaattgca aagcttcaat ggtagggtgt
     3001 aaaacagcac cgccaagaga cttgttatag gcagtactac cagtcggtgt cgaaactgtt
     3061 agcccgtctc cacgaaaacg ttcaaaggga acaccattta ttacaatatc tgccaccatg
     3121 gttcgatcag acctgcggat gctggcttcg ttgagtgctc tgaaaatctt aacttcacca
     3181 ttttcaagaa agaccttcac attcagaaca gggtaagaaa cccttgcccc agtatctagc
     3241 tgcaaattag tcactagctt gtccaactca aaatcacgat aatctgtata gaagcccaaa
     3301 tgtccagtat gaagaccgat aaagcggacc ttgtcaagct gattttcgta cttatgaaag
     3361 gccgacaaga gcataccatc cccgccaatg gaaatgacaa tatccggatt ggtatcattg
     3421 agtataaact gatttctctt caaacgatct cgcaattcat acaaaaccct ttgactctgc
     3481 ggttttctat tggctatcag atcaattcgt ttacctgtat tcttcatctg tatcgtcact
     3541 gtttcctaca ccgtcattta attttctact caaaggatca aaaagtgcct gggcttcttg
     3601 gatatcatca cgaatttcac ccatttcttc atccaactga tgggcgattc tagctgtaat
     3661 ttccagtcgc ttcttaatct catctgggaa atccccttgg tacttgtagt tgagagaatg
     3721 ttctatcgtt gcccagaaat tcatggccaa agtacgaatt tgaatttctg ccaaaatagt
     3781 cttagctcca ttgatggtat caaccgtata ttctactacc acatgatagg aacgatagcc
     3841 tgatgctttt ctatgagtaa tgtaatctcg ctcctgtatg attcgcatat cctgacgctt
     3901 gtgcaaaata tccactactt ccttgacgtc atctacaaac tgaaccatca cacgtaagcc
     3961 agcaatatcc tgcaaatcgt gttccaaggt cgcataagta atgccacgac gagccatttt
     4021 ttctttgatg ctctcaattg gcttaactcg accggtcaca aactcaattg gagaatgctt
     4081 attttgctta cgatattgct tacgaatacc acgaagttta atctttaact caccaacagc
     4141 ttgaatgtaa ggatctagaa attcttccca ttctaaggtc atatattctc ccttgttctc
     4201 atattatccg tcaaaaatat ctttgatttt ttctccagat tcatatacaa taaatacaat
     4261 gcttattata ccataaatcc gctttcaacg ataaaaaaag gtaaaaaaaa tgaaacattt
     4321 agaaattgaa ttgaaaacac tattgaaaaa agatgaatac aatcgtctaa aagaccagtt
     4381 cacaggtgtc actcctgttc ttcaaacaaa ttactacatc gacacgcctg attttgaact
     4441 gcgagaaaag aaagtcgcta tgcgcattcg aacctttgaa gactgggctg agttgacact
     4501 caaagtcccg cagagtgttg gaaacatgga atacaaccaa aaattacaac taaaagatgc
     4561 tgagaactat ctgaataagg aagaacttcc tcaagggcta gtactggatg aattggcgaa
     4621 acatggtatc caaagtaaga actggcaggt tcttggttgt ctaacaacgc ttcgctatga
     4681 aatgaaaaca gctattggtc tcatggcact ggatcagagc cgatactttg atataacaga
     4741 ttacgaattg gagcttgaag tggaaaatca tgagcaaggc aaacaggatt tccgacaatt
     4801 tttagagaaa aatcagattt cctaccaaaa agctccttca aaattggttc gatttgtcaa
     4861 aagtatgaaa aatagctgaa ataatctcca ttttttggta aaatagaaaa gataaaaata
     4921 cacaaatccc agttcatata tgtaaggtaa atataactag gatttataaa gtttacagag
     4981 gacggtctat aatgtcagat agaaaaaaca tgaaactttt cgcactcaac tctaaccaag
     5041 agattgcaca aaaaattgcc caagctgttg gtgtcccact tggaaaacta tcatcacgtc
     5101 aattttcaga cggagaaatc caagtaaata tcgaagaaag tgtccgtggt tatgatgttt
     5161 acatcatcca atcaacaagt ttccctgtca acaaccacct aatggaattg ttaatcatgg
     5221 tcgatgcttg tgtgcgtgca agtgcccaca gtatcaacgt tgtccttcca tattttggct
     5281 atgcacgtca agaccgcatt gcttgtcctc gtgagccact tacagcaaaa ctagttgcca
     5341 atatgctggt taaggctgga gttgatcgta tcctgactct tgatttgcat gccgttcagg
     5401 ttcaaggttt ctttgatatt ccagtggata atcttttcac tgttccccta ttcgcaaaac
     5461 attactgcga taaaggattg cttggttcag atgttgttgt cgttagccct aaaaattcag
     5521 gtgtcaaacg tgcgcgtagc ctggctgaat atcttgatgc tcctatcgcc attatcgact
     5581 accctcaaga cgatgcaact cgtaacgaag gttatattat tggtgatgtt gaaggtaaga
     5641 aagctatctt gattgatgat attttaaata caggacgtac cttctctgaa gcttctaaaa
     5701 tcgttgaacg tgaaggagct acagaaattt atgctgtttc tagccacggt ctcttcgtcg
     5761 agggagctgc tgagcttctt gacaatacta atattaaaga aattcttgtg actgattcag
     5821 tagcaacaaa agaaaaaact cctaaaaatg tatgctacat cactgctagt gagttaattg
     5881 gtgatgctat cgtccgtatt cacgaaagaa aaccagtcag cccactcttt gcctacaata
     5941 aaaagaaata aggtgattct ttgatttatt tggacaatgc tgcaacgact cctatgtcag
     6001 cagttgctat ttcagctatg accaaggtta tgcaagaaac ccacggaaat ccttctagta
     6061 ttcatggtca tggtcgtcaa gctggcaaac tcttgcgaga agcccgtcag gaactagccc
     6121 agttactaag gacaaaacct caacatatct ttttcacttc tggtgggact gaaggcaata
     6181 atactaccat cattggctac tgtcttcgtc accaagaaca aggaaaacat atcatcacaa
     6241 ctgccatcga gcaccatgct gtccttgaaa caattgatta cttggttcaa cactttgggt
     6301 ttgaagcaac cattatccag ccagaaaatc aagaaatcac agcccagcaa attcaaaagg
     6361 ctttacgtga cgatacgatt ttggtttcta ccatgtttgc caataatgag acaggaaacc
     6421 tactgcccat cgctgaaatt ggccaaatac tcaagcaaca ccctgctgcc tatcatgttg
     6481 atgcagttca ggctattggt aaaatcccaa ttcattcaga agaattgggc attgattttc
     6541 tcactgcttc tgcccacaaa ttccatggtc ctaagggaat cggttttctc tacgcatcta
     6601 gtatggactt tgattcctat ctacatggcg gagaccagga acagaaaaaa cgtgcaggaa
     6661 ctgaaaatct gcctgccatc gtaggcatgg ttgcagccct aaaagaagac ctagaaaaac
     6721 aagaagaaca ttttcaacat gtacaaaatc tagaaactgc ctttctggca gagctggagg
     6781 gcattcagta ttacttgaat agaggaaaac atcatctccc ttatgttctc aatattggat
     6841 ttcctggtca gaaaaatgac ctcttactcc ttcggctaga tttagctgga atttcaatct
     6901 ctactggctc agcctgtact gcaggcgttg tccaatccag ccatgttctt gaagccatgt
     6961 atggcgcaaa ttcagaacgc ttgaaggaat cccttcgcat cagtttgtcg ccacaaaata
     7021 ccgttgaaga cctacaaacc ctcgcaaaaa ccttaaaaga aattatcgga ggttagccat
     7081 atggcatttg aaaaaatcat tcagttaaaa aattgtcgtt acgattacac tcttagccct
     7141 tctgttaaaa aattcaccct caaagataac accttttttg aaactaaggt tggtaactat
     7201 gaactgactc gccttttgga aaaagtgcca aacagcggtg aaggcttcca actcaaaatc
     7261 atcattaaca aggaacttac agggactaaa atcaatatca ctgacaagtt tggccttcgt
     7321 ctagttgata ttttcaaatc agaagaccac catattcatc aggaaaaatt ctacttcctc
     7381 atggatagct tggtagaacg tggtgtcttt acaaaatcgg aaagatagag ccaaaatgtt
     7441 tgaactgacc tataaggact gctatcatgt agagcgtact ctcaagtatg aggatcatga
     7501 ggctctcatg ttgactttgt caggatgtgt aaccttacca gatacacttt atgtgacttc
     7561 tctgactttt aggggccaaa aagtttatca gggtctggtc ggagacctct accgttttct
     7621 atctcatgca gattttttac atcaaaacta agatttttct tagttttttc ttatttttgt
     7681 tgattttttc acaatgtttc tataaaatag aagaatagaa aggttgtgat tttgtgaaag
     7741 ataaacagtt tgctattcca aaagctacag caaaaagact ctctctctac tatcgaattt
     7801 ttaagagatt tcatgcagaa aagattgaac gtgccaactc taagcaaatt gcagaggcca
     7861 ttgggattga ttcagcgacc gtacgtcgtg atttttccta ttttggtgaa cttggtcgtc
     7921 gtggttttgg ctatgatgtc aaaaaactaa tgacattttt tgccgatttg ctcaatgaca
     7981 actctattac caatgtcatg ctggttggta ttggaaatat gggccatgcc cttctccact
     8041 accgcttcca cgaacgtaac aagatgaaga ttatcatggc ctttgaccta gacgaccatc
     8101 ctgaagtcgg tacccaaact cctgacggga ttcccattta cgggatttct cagatcaagg
     8161 ataaaatcaa ggatgctgat gtgaagactg ctatcctaac tgttcccagc gtcaagtcac
     8221 aagaggttgc taatctcttg gttgatgctg gtgtgaaagg aattctcagt ttttcaccag
     8281 tccatctgca tttaccaaaa gacgtggtcg ttcagtatgt cgatttgaca agtgaactcc
     8341 aaaccctcct ctacttcatg cgaaaagagg attagaaagc gaaaatcatt ttatgaaaaa
     8401 accagttatt gggattacag gaaacgaaaa aactcatcca gatgatgaca tcatgatgag
     8461 ctacgcagca aaaggctttg ttgaaggcgt taaagatgct ggagggattc ccatcatcct
     8521 accgattggt gatcaagaaa tggcctgcca ctatatcagt ttgattgaca agctcatctt
     8581 aacaggtggg caaaatgtcg atccaaaatt ttatggcgaa cccaaaacca ttgatagcga
     8641 tgactatcac cttcaaagag atatcttcga actggccctc atcaaggaag ctattaaaca
     8701 gaaaaagccc attttctctg tctgccgtgg tacccaactc tttaacgttg ccatgggtgg
     8761 aactttgtac caagatatcg aagaccattg gcaggattct tctgttgagt acacaactca
     8821 acgcttggtg acagaaccag ataccgttct ccaagaaatc tatggagaaa tctctcatat
     8881 caactccttc caccaccaga gcatcaagga cttagcacca aatttaaaag ttgtggctca
     8941 tgatcctaaa gatggtatca ttgaggctgt catgagtacg gatgatgtcg cctttctcgg
     9001 tgtccaatgg catccagaac ttctttttga aaatcgtccc aaagataaga aactctttga
     9061 ctatgtcgtt aatgaacttt agattaaatc tgtcttttca cgataactaa agtaattaga
     9121 atgagagaca atcaaatggt ccaagagaac aattcccatc agttcgcagg cttctttaac
     9181 aagtttagtg acatgatcat cattttggct aggcgctacc gctcctgaag gatgattgtg
     9241 gaccaagata agagaagtcg ccatatgctt gattgcatag tgaagaatct ctcgcggttc
     9301 agcgatacta cgagttacag aaccgataaa aatggtctgc tgatggatga tttgattttg
     9361 agtattgaga tagagtgcca ccaggtgctc ttgtttttta tcccctaatt cctgctgcat
     9421 cttcttggcc aacttttgac tgctgagaat actttccatt tcaagagtct cgtgtttgtg
     9481 aatacgatgc cccagttcaa tcatagcttg taattctatg gccttaacac gcccaatacc
     9541 agacaaactc tgcaattcct gcagggtcat ttttttcaaa tccgttaggc ttgaaagatt
     9601 gttcaagact ttttgggcaa tttcaaaaac gctaacttga cgtgttcctg tcctgagtaa
     9661 aatagctagc aactcttggt tactaagcgc ttcaactcct tccttggcca gcctttctct
     9721 tggtaatagt gaatcttctt ggaatgaaat actgtacata aaaatcctcc tcactttatt
     9781 attcgtgaga aggatggaaa attagatttt tttctcaatt ggagccacac tggctaaaag
     9841 ttttttcaaa cctacttctg ggaaattgat tttcaattcc tgcctagcac cgctacctga
     9901 aacttccaga acggttccct ctccccattt cttgtggaga gcaatatcac caatggacca
     9961 atttgcctcg ctagatgctg gttttgcgcc agctgtaaat tgaccaaatg gaagaccgct
    10021 tgactggatt gattttgggg cagcaccgcg tttacggtct tgaagagcct gagccaaact
    10081 cataccttga ccaaaggaaa tactaccact gctatatgat gccttaaagc ttgtatttgc
    10141 aggacgagcc agaccttgat actcaagcaa gtctgaactg atttcgttaa taaaacgagt
    10201 cggacggtta taattggtac gaccaaaaag caagcgtgag ttggcattgg tcagatagag
    10261 aattttctct gcacgcgtga tacctacata ggctagacgg cgctcttctt ctaattcatc
    10321 tggatcttca gtcgcacgac taagtggaaa gacattttct tccatcccaa tcaaaaagac
    10381 aactggaaat tcgagacctt tggcagcatg cagggtcatc aaggtcactt ctgatgtctc
    10441 ctgactacct gaatctgtgt cggcaatcaa agccaagtca tttaagaaac gactcagttt
    10501 gtccagacca gtttcctctt ctgtcacatc cgtggtgtca tcaaagttct tcgtaacaga
    10561 aagaaactct tcgatatttt caacccgtgc cttgctttct agagtcgctt gggcgttaag
    10621 aatatcgaca taacctgttt tttctaggac ggactcaacc aactctgtaa tgcttaagtg
    10681 gtctagctgc tcccgcaaat caagcatcat attggcaaaa tcccagatag attgggctgc
    10741 cttacccttg ataccagaca acataatatt agcagaagca tccagcatag acatattttg
    10801 caaatttgca aaatcacgga ttttctcaac tgtacctaga ccaattccac gtttaggctc
    10861 gttgataata cgctcaaaac taatattgtc actcaaatta gcaataaggt tgagataagc
    10921 aataatatcg cgaatttcct tacggctgta gaatttggtt ccgccaacca tggtataagg
    10981 aatgttagac ttgagcaggg cttcctcaat tgtacgggac tgggcattag tccgatagag
    11041 aactgcaaaa tccttatgaa ggaagttttg actgcgacta agttcatcga tggttctggc
    11101 tacaaataca gcctcatcca gctcatcatc ggcacgatag taaacgattt gctctccatc
    11161 agcgttttga gtccagagat ttttaggacg gcgattttta ttatttttaa taacctcgtt
    11221 ggccgcttgg agaatggttt tggttgagcg gtaattttcc tccagcaaaa caaccttggc
    11281 tttggggtaa tccttttcaa agtccaagat attctgcata tcagcaccac gccaaccgta
    11341 gatagactgg tccgcatccc caaccacaca gatattttta aaacgggaag ccaagagttt
    11401 gaccaattgg tactgagcgt ggttggtatc ttggtactca tcaacgtgga tgtattggaa
    11461 tttttgctgg tagtaggtca aaacatcagg attttgatca aagagacgca aggtcagcat
    11521 aatcaaatca tcaaagtcaa cggattcaga ctgacgaagt tctttttgat aggctgtata
    11581 acactgggcc acaatttgcg tatacatatc gccagcttgg gcagcataag caacatcatc
    11641 aatcaaatca ttcttagcat tggaaatggt ccccaaaata gttcgttcat tccgtttttt
    11701 aggatccaag ttcaactgtt tgagaatacg tttcatgagc gttcgctgtt caccaggatc
    11761 cacaattgta aaattacgat tgtagccaat atggtccgca tcgcgacgca aaatacgcac
    11821 acacatggag tggaaggtcg caatcagaca gtcctgagta gctggattga ggctataagc
    11881 acgctctttc atctcacgcg cagccttgtt ggtaaaggta atggccaaga tattccaagg
    11941 attgaccagc ttttcatcaa tcaaataagc gatacggtgg gttaaaacac gagtctttcc
    12001 agaaccagcc cctgccatga ttagcaaggg accttctgtc gtttgcaccg cctcagcctg
    12061 acggtcattc attccattta ataatgcgtt catcttctct tccttactct tgaagtcaat
    12121 atttctatta tatcagaaat cagggaaaat agaaacttta cggggagtag caaaatatag
    12181 tggattgaaa ctagaatact acactgtggg tttctaaaac actcgacttt tcacccaatt
    12241 ttttcataaa ctagctcaat acctcaatag acttcctgca aaactagaat cctagttcat
    12301 gattgataat accagcactc aaattcattc gtaatccgaa gcgtttacga tgacttcgat
    12361 aggttgttga aaacatttta aacgttttta ctttggcaaa gatgttctca accttgcttc
    12421 tctccttaga tagcgcatgg ttataagctt tatcttcagc tattag
//
LOCUS       DAVKJD010000053        12374 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKJD010000053 DAVKJD010000000
VERSION     DAVKJD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12374
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.053"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..748
                     /locus_tag="VW157_001478"
     CDS             <1..748
                     /locus_tag="VW157_001478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552760.1"
                     /translation="SNYAYILSIVYIFFGGIFNPIQASIGNLSLLASIEKKNEIFNRL
                     RFINFFFLSFCSSCLLVLVNPFITIWIGENYTLSFTGVIAIVGMLFVRQTGNCTTIFR
                     LGEGYFRDYHFSPLIAGILNLVVSVILVNYIGIAGVFVGTMLGFGFQFILVDTIVTYK
                     KVLSRPLSEFYLRWLQTILLTVGLCIASYYLSRLVRVNSIYDLILLFVVVIGFNFFAL
                     CFIYWRNDDFQYFIQLVKNFMKNLEEKNHD"
     gene            741..1964
                     /locus_tag="VW157_001479"
     CDS             741..1964
                     /locus_tag="VW157_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010722986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU9552761.1"
                     /translation="MINVIGLGYIGLPTILMLATNGVKVVGTDYNQDLVRTLNEGQTT
                     FKEDGLDELFHKAVESGVDFTTEYQQTDTYIISVPTPYDSFSKKIDPSYVIEATKTVL
                     DNCNKGAVIIIESTVSPGTVDKFIRPVVEEKGFVIGSDIHLVHAPERIIPGNMVYELV
                     NNNRTIGADDLEIGYKVKELYASFCKGDIVVTDIRTAEMTKVVENTFRAVNIAFANEL
                     AQICSYDNMNVYEIIRICNMHPRVNILQPGPGVGGHCISVDPWFLVGDYPELTNVIEH
                     SMRTNAAMPEFVLNRIYTIMNEHHLTDISRVGLYGLTYKENVDDMRESPTLQLLESMS
                     RHLATPAIKVYDPFIEKDVVANQSHDLDEFLSNVDIVVLLVGHDEILQNMDKLKDKIV
                     LDTRYICHLDGTYRL"
     gene            1993..3090
                     /gene="wecB"
                     /locus_tag="VW157_001480"
     CDS             1993..3090
                     /gene="wecB"
                     /locus_tag="VW157_001480"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU9552762.1"
                     /translation="MKKIVVIFGTRPEAIKMCPLVKELRTRENLETLVCVTGQHRQML
                     DQVLDTFRITPDFDLSIMKDKQTLFDVTIGILEGMKTVLESVNPDLILVHGDTSTTFA
                     SSLAAFYLQIPIGHVEAGLRTYDIYSPYPEEFNRQAVGALAQYHFTPTQLSKDNLLRE
                     GKNSEAIFITGNTAIDALQTTIQEDYTHPELNWVGDSKLILITAHRRENIGDPMRHMF
                     RAIRRIIEEHSDIKALYPIHMNPLVRQIAEEELSGCERIKMIDPLEVLDFHNFLSRSY
                     LILTDSGGIQEEAPSLGKPVLVMRDTTERPEGIKAGTLKLVGTDEDNIYRHFKELLEN
                     DLVYQAMSQASNPYGDGTACKKIADILEGEV"
     gene            3094..4149
                     /gene="cps4J"
                     /locus_tag="VW157_001481"
     CDS             3094..4149
                     /gene="cps4J"
                     /locus_tag="VW157_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4J"
                     /protein_id="HEU9552763.1"
                     /translation="MSQFTGKTLLITGGTGSFGNAVLNRFLQTDIGEIRIFSRDEKKQ
                     DDMRHEFQVKVPEVAGKIRFYLGDVRDLASVKNAMHGVDYVFHAAALKQVPSCEFFPV
                     EAVKTNILGTENVLTAAIEAGVKQVICLSTDKAAYPVNAMGTSKAMMEKIAVAKSRTV
                     NPEHTKICVTRYGNVLCSRGSVVPLWIEQIKQGNALTITEPSMTRFVMTLEEAVDLVL
                     FAFEEGKSGDILVQKAPACTIEVLAKAVSEIFASEQDIKIIGIRHGEKRYETLLTNEE
                     CANAIDLGDFYRVPSDNRNLNYDKYFKDGSTNRNLLTEFNSNNTDLMDVEQVKRKLLE
                     LDEIQTAIRDMVADEEM"
     gene            4249..5478
                     /gene="cps4K"
                     /locus_tag="VW157_001482"
     CDS             4249..5478
                     /gene="cps4K"
                     /locus_tag="VW157_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4K"
                     /protein_id="HEU9552764.1"
                     /translation="MIKNILITGAKGFVGKNLICTLEALKDGRDRTRPNLEIGEIFQY
                     DRDTDPILLDEYCKKADFVFHLAGVNRPQNPDEFMEGNYGFSSRLLEILEKYENTCPV
                     LLSSSTQASLEGRFSNSIYGQSKLAGEELFFEYGKKTGAPVLVYRFPNLYGKWCRPNY
                     NSAVATFCHNLAHDLPIQVNDPSVELELLYIDDLIQECLTALEGNPHRCNLDGLQILP
                     SPSGNYCYVPTTHRATLGEIVSLLETFKKQPNSLVMPEIPQGSFKKKLYSTYLSYLPV
                     DKFRFPLKMNIDERGSFTELLKTENTGQFSVNISKPGITKGQHWHHSKWEFFMVVSGR
                     ALIQERRIGLDENGQEYPILNFEVSGDKIEAIHMIPGYAHNIINLSDTENLITVMWAN
                     ESFDPRHPDTFFEQVEK"
     gene            5479..6663
                     /gene="cps4L"
                     /locus_tag="VW157_001483"
     CDS             5479..6663
                     /gene="cps4L"
                     /locus_tag="VW157_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4L"
                     /protein_id="HEU9552765.1"
                     /translation="MKIKTDYSDIHFKDNGKLKLLIIVGTRPEIIRLSSVITKCRKYF
                     DVILAHTGQNYDYNLNGIFFDNLGLDTPDVYMDAVGDDLGATVGNIINTSYKLMNQIK
                     PDALLILGDTNSCLSAIAAKRLHIPIFHMEAGNRCKDECLPEETNRRIVDIISDVNLA
                     YSEHARKYLHECGLPKERTYVTGSPMAEVLHKNLSAIESSDIHERLGLKKGGYILLSA
                     HREENIDTDKNFISLFTAINQLAEKYNMPILYSCHPRSKKRLQESGFKLDKRVIQHEP
                     LGFHDYNCLQMNAFVVVSDSGTLPEESSFFTSQGYPFPAVCIRTSTERPESLDKAGFI
                     LAGIDENSLLQAVETAVSLAEDEDFGLPVPDYVEENVSTKVVKIIQSYTGIVDKIVWR
                     KS"
     gene            complement(7254..7806)
                     /locus_tag="VW157_001484"
                     /pseudo
     CDS             complement(7254..7806)
                     /locus_tag="VW157_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7882..8109)
                     /locus_tag="VW157_001485"
     CDS             complement(7882..8109)
                     /locus_tag="VW157_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001815354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9552766.1"
                     /translation="MRERLLTQPLLHADETSYRVLESDSQLTYYWTFLSGKSEKQGIT
                     LYHHDQCRSGSVVQEFLGDYSGYVYCDMLRQ"
     gene            8137..8445
                     /locus_tag="VW157_001486"
                     /pseudo
     CDS             8137..8445
                     /locus_tag="VW157_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8600..8923)
                     /gene="tnpB"
                     /locus_tag="VW157_001487"
     CDS             complement(8600..8923)
                     /gene="tnpB"
                     /locus_tag="VW157_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9552767.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDSFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPKI"
     gene            complement(8904..9089)
                     /locus_tag="VW157_001488"
     CDS             complement(8904..9089)
                     /locus_tag="VW157_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552768.1"
                     /translation="MLQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MIEQLLDKVLLYDNSSI"
     gene            9153..9963
                     /locus_tag="VW157_001489"
     CDS             join(9153..9527,9529..9963)
                     /locus_tag="VW157_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9552769.1"
                     /translation="MPCYDALTKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAK
                     GGRKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLIRRSQWVEVTLVQSGVTI
                     SRTPLSSEDTVMIDATEVQINRPKKELANHSGKKKFHAMKAQAIVTSQRRIVSLDITV
                     NYCHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTAEDKACN
                     HALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(9983..10109)
                     /locus_tag="VW157_001490"
                     /pseudo
     CDS             complement(9983..10109)
                     /locus_tag="VW157_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            10227..12209
                     /locus_tag="VW157_001491"
     CDS             10227..12209
                     /locus_tag="VW157_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9552770.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT         3992 a         2023 c         2501 g         3858 t
ORIGIN      
        1 ttcgaactat gcttatattt tatcaatcgt atatattttc tttggaggaa tttttaatcc
       61 gattcaggct tctattggaa acctatctct gttagcttct atagagaaaa aaaatgaaat
      121 cttcaataga ttacgtttta taaatttttt ctttttatca ttttgctcaa gttgtcttct
      181 agtattggta aatcctttta tcactatttg gattggagaa aattacactt tatcatttac
      241 aggtgtgatt gcaatagttg gtatgttatt cgttagacaa actggaaatt gtacaaccat
      301 ttttcgtttg ggagaaggat attttagaga ttatcatttt tctcctttaa tcgcgggcat
      361 tttaaattta gttgtttcag taattttagt gaattatata ggtattgcag gtgtttttgt
      421 tggaacaatg ttaggatttg gtttccaatt tatactggtg gatacaattg ttacttataa
      481 aaaagtactt agtcgtccac tatcagagtt ttatttaaga tggttgcaga cgatcttatt
      541 aactgtaggt ttatgtattg cgagttacta tttatctcga ttggttagag tgaatagtat
      601 atatgatttg atactgttgt ttgtagtggt tattggattt aatttttttg ctttatgttt
      661 tatttattgg cgaaatgatg attttcaata ttttattcaa ttggttaaaa attttatgaa
      721 aaatttggag gaaaaaaatc atgattaatg taatcggatt aggttatatt ggattgccaa
      781 caattttaat gttggcaaca aacggcgtga aagtagttgg aactgactac aatcaggatt
      841 tggtgagaac tttaaatgaa ggtcaaacaa cctttaaaga agatggattg gatgaactat
      901 tccataaagc agtggagtcg ggtgtggact ttacaaccga atatcaacaa acggatacct
      961 atattatttc cgttccaaca ccatatgatt ccttctctaa aaaaattgat ccaagctatg
     1021 tgattgaagc tacgaaaacg gtacttgata attgcaataa aggagcggtt attattattg
     1081 aatcgaccgt atcaccagga acggttgata aatttattcg acctgttgta gaagaaaaag
     1141 gttttgttat tggtagtgat attcatcttg tccatgctcc agaacgtatt attccaggga
     1201 atatggtcta tgaattggtg aataataacc gtacaattgg agctgatgac ctagaaatcg
     1261 gctataaagt gaaagagctt tatgcttcat tttgtaaggg cgatattgtt gttacagata
     1321 ttagaactgc ggagatgacc aaggtagtag aaaatacatt tagagctgta aatattgctt
     1381 tcgctaacga attagcacag atctgtagtt atgataatat gaatgtttat gaaattatcc
     1441 ggatctgtaa tatgcaccca cgggtaaata ttctacaacc tggacctggt gttggtgggc
     1501 attgtatttc tgtggatcct tggttcttag ttggagatta tcctgagttg acaaatgtta
     1561 ttgaacattc tatgcgaaca aatgctgcca tgccagaatt tgtattaaat cgtatctata
     1621 ccattatgaa cgaacatcat ctaacagata ttagtcgggt tggtctatat ggtcttacct
     1681 acaaagagaa tgtagatgat atgcgtgaat ctccaacgct tcagttatta gaatctatga
     1741 gccgccatct ggcaacacct gctattaaag tatatgatcc atttatagaa aaagatgttg
     1801 tggcgaatca gtcacatgat ttagatgaat ttttatctaa tgttgatatt gttgtactgt
     1861 tggttggtca tgatgagatt ttacaaaata tggataaact aaaagataag atagtgttag
     1921 atacacgtta tatctgtcat cttgatggaa cttacagatt ataattagtg ataaataaag
     1981 taaggattga ctatgaaaaa aatagttgta attttcggaa ctcgtcccga agctatcaag
     2041 atgtgtccct tggttaaaga gctaagaaca cgtgagaatc ttgaaacatt ggtctgtgtt
     2101 acgggtcaac atcgtcaaat gctggatcaa gttttggata cttttaggat tacgcctgat
     2161 tttgatttat ctattatgaa ggacaaacaa actctctttg atgtcacaat tggcattttg
     2221 gaaggcatga agactgtctt agagtcagtt aatcctgatt taatattagt tcatggggat
     2281 acgagtacaa catttgccag ttcactggcc gccttttatc tacagattcc aattggacat
     2341 gtagaggctg gtttgcgtac ttatgatatt tattcacctt atcctgaaga atttaatcgt
     2401 caagcagtag gtgctttggc tcagtatcat tttacaccaa cacaactctc aaaagacaat
     2461 ctcttgagag aaggaaaaaa ttctgaagct atatttatca caggtaacac agcaattgat
     2521 gctttacaaa ctacaattca agaggattat actcatcctg agttaaattg ggttggggat
     2581 agcaaactaa tattaattac tgctcatcgt cgtgaaaata ttggtgaccc gatgaggcac
     2641 atgtttagag caatccgacg tatcattgaa gaacattcgg atataaaggc tctttacccc
     2701 attcatatga atcctctagt tcgtcagatt gctgaggaag aactaagcgg atgtgaacgt
     2761 atcaaaatga tcgatccgtt ggaagtatta gattttcata actttttatc acggagttat
     2821 ctcattttaa cagatagtgg tggtatccaa gaagaggcac ctagtttagg aaaacctgtt
     2881 ttagttatga gagatacaac tgaacgtccg gagggaatta aagcaggaac tttgaagctt
     2941 gtaggtactg atgaggacaa tatttataga cattttaaag aattgttaga aaatgattta
     3001 gtttatcaag cgatgagcca agcttcaaat ccgtatggag atggaacagc ttgcaagaaa
     3061 attgcagaca tactagaagg agaagtataa tatatgtcac aatttacagg aaaaactctc
     3121 ctaattacag gaggaacagg ttcatttggt aatgctgtcc ttaatcgttt tttacagact
     3181 gatattggtg aaattcgtat tttttcccgt gatgaaaaaa aacaggatga tatgcgtcat
     3241 gagtttcagg taaaagtacc agaagtagct gggaaaatcc gtttttacct tggagatgtg
     3301 cgtgacttag cttcggtaaa aaatgctatg catggtgtgg attatgtttt tcatgctgct
     3361 gctcttaaac aagtaccttc gtgtgaattt ttccctgtag aagcggtaaa aactaacatt
     3421 ttgggaacgg agaatgtcct cactgcggct atcgaagctg gtgtaaagca agttatctgt
     3481 ctttctacag ataaggcggc ttatcctgtc aatgctatgg gaacatccaa ggctatgatg
     3541 gaaaagattg ctgttgctaa gtccaggacg gtaaatcctg agcatacaaa gatatgtgtg
     3601 actcgctatg ggaatgttct gtgtagccgt ggatctgtcg ttcctctttg gattgagcaa
     3661 attaagcaag gaaatgctct tacaattact gaacctagca tgacacgttt tgtgatgaca
     3721 ttagaagaag cagtagactt ggttttattt gcatttgaag aagggaaatc tggagatata
     3781 ctagttcaga aagcaccagc atgtacgatt gaagtgttgg ctaaggctgt atcagaaatt
     3841 tttgcctcag aacaagatat taagattata ggtattagac acggagaaaa aaggtatgaa
     3901 acactattaa ctaatgaaga gtgcgcaaat gcaattgatt tgggtgattt ttatagagtt
     3961 cctagtgata atcgtaatct taactatgat aagtatttca aagatggtag tacgaataga
     4021 aatttgttga cagaattcaa tagtaataat accgatttga tggatgtaga gcaagttaaa
     4081 agaaagttgc tagaattaga tgaaatacag acagcaattc gtgatatggt agcagatgaa
     4141 gagatgtaaa atcttttaag atacattaaa attagcaagg atatataatg tatcatgtat
     4201 tgtcgagaga gttgactatt aaaacagcat ataaaacaag gagggggcat gattaaaaat
     4261 attttaatca caggagcgaa aggatttgta ggaaaaaatc ttatctgtac tctggaagct
     4321 ttgaaagatg gacgagatag gactcgtcct aatttagaga ttggagagat ttttcagtat
     4381 gatcgtgata cagatccgat tttattagat gaatattgta agaaggccga tttcgtattc
     4441 catttagctg gtgtcaatcg tccacagaat cctgatgaat tcatggaggg aaattacggt
     4501 ttttcaagta gattattgga gattttagaa aagtatgaaa acacttgtcc tgttctactc
     4561 tcaagttcta ctcaagctag tttagaaggc cgattttcaa actctatata tggacaatct
     4621 aagctagcag gggaagaact cttctttgaa tatggaaaga aaacgggagc acctgtctta
     4681 gtttaccgtt tcccgaatct ttatgggaag tggtgccgtc ctaactacaa ttctgctgta
     4741 gcaactttct gtcataatct agctcacgat ttacctattc aagtaaatga tccaagtgta
     4801 gaattggagt tgctgtatat tgatgatttg atacaagagt gtctaactgc attggaagga
     4861 aatcctcatc gttgtaatct agatggatta caaatcttac ctagcccatc aggaaactac
     4921 tgctacgtac caacgactca tcgtgcaacc ttaggagaga ttgtctctct attagaaaca
     4981 tttaaaaaac agcctaatag tttagttatg cctgaaattc ctcaaggatc gtttaaaaag
     5041 aaattgtatt ctacctacct atcttattta ccagtagata agtttaggtt tcctctaaaa
     5101 atgaatatag atgaacgagg tagttttacg gaattattaa aaacagaaaa tacgggtcaa
     5161 ttttctgtta atatttctaa acctggcatt accaaagggc aacattggca tcattctaag
     5221 tgggaatttt tcatggttgt ttctggtcgt gctttaatac aagagcgtag gatagggctg
     5281 gatgaaaacg gtcaagagta tcctattctg aactttgaag tgtcgggcga taagattgaa
     5341 gcaatacata tgataccggg ctatgcacat aatattatta atctttctga tacagaaaat
     5401 ctaattactg tcatgtgggc taatgagtca tttgatccta gacatccaga tacttttttt
     5461 gaacaagtgg agaaataaat gaaaattaag acagattata gtgatattca ttttaaagat
     5521 aatggcaaac ttaagttatt gattattgtg ggaacacgtc cagagattat acgtctaagt
     5581 agtgttatca ctaaatgtcg aaagtatttt gatgttattt tggcacatac tggacaaaac
     5641 tatgattaca atttaaatgg tattttcttt gataatttag ggttagacac tccggacgta
     5701 tacatggatg ctgttggaga tgatcttggt gctactgtag ggaatattat taatacttca
     5761 tacaaattga tgaatcaaat taaaccagat gctttattga ttttagggga tacaaattct
     5821 tgtttatcag ctattgctgc caagcgttta catattccaa tttttcatat ggaggctggc
     5881 aatcgctgta aagatgagtg cctgccggaa gagactaatc gtcggattgt tgatattatt
     5941 tcagatgtta acttagcata ctctgaacat gcacgtaagt atttacatga gtgtggttta
     6001 cctaaagagc gcacatatgt aactggttct cctatggcag aagtgttaca taaaaattta
     6061 tctgccattg agtcttcaga tatccatgaa cgtttgggat tgaaaaaagg aggttatatc
     6121 ttactttcag ctcaccgtga ggaaaatatt gatacagata aaaattttat ttctctcttt
     6181 acagcaatta atcaattagc tgaaaagtat aatatgccaa tcttatattc ttgccatcct
     6241 agatcaaaga aaaggctaca agagagcggt tttaaactag ataaacgtgt gattcagcat
     6301 gagccactag gattccatga ctataattgt ttacagatga atgcatttgt tgtagtatct
     6361 gattcgggaa ctttaccgga agaaagtagt ttctttacta gtcaaggtta tccttttccc
     6421 gctgtatgta ttcgtacaag tacagaacgt cctgagtcct tagataaggc aggatttatt
     6481 ttggcaggca ttgatgaaaa ttctcttctt caagcagttg aaactgctgt tagcttggca
     6541 gaagatgagg attttggatt accggttcca gattatgttg aggaaaatgt ctctactaaa
     6601 gttgttaaga taatacagag ctatacaggg atagtggata aaatagtttg gaggaaaagc
     6661 tgaattgtat atttttaata aatcaaaacg agatgattaa ttcttagtag aacaagagta
     6721 aaattgaacg tatttgatgg aaatgtctta cttgatagtc gagggatagc cattctatcg
     6781 gatttttggc ttgtttttgc gcttcatgtt tgatagaata aagggctctt tgtcaactgt
     6841 agtgggttga agaaaagcta agcttgagaa aggacaaatt tcgtcctttc ttttttgatg
     6901 ttcagagcga taaaaatccg ttttttgaag ttttcaaagt ttcgaaagta accgcccaat
     6961 aacgaagtct attgaaaaat ctccagacta gagaactcac ggatagttcc taatctggag
     7021 atttcttatt tgcacttttc ttgtacaact ttagtccatg gtaaataagc ctctaaaacc
     7081 tctttgttta cgagagtttc ctcgtttgga agacattcta gaagatagga tagatatttc
     7141 tcgctattta taatggattg aaataagata tgaacaaatc gattaggaaa ggaaaattga
     7201 tttatagaaa tattttagca gtcgatgcgt actgttatag attcaacgca ctataattga
     7261 tgacgtttag ctgtttccaa caaactcata ataatagctc ttgctttagc tccttcaaaa
     7321 ctttgagaaa ataaccaccc tcgctttttt aaactgagct caggctaaaa gagtccactg
     7381 gactctttta ctccgtccca taaccaatga tttaatggcg cgttcagcta gattattgga
     7441 aaggaccaga tgtccgtctt ttaaaatggt cttaaaggtt tctttatact tgaggctgta
     7501 ttcaattgcc cttcctagtt ttgaacctgc taaaactgac tgacggcggc accaagcaaa
     7561 gaagtcttcc attaggggct ggagatgttc ttgacgtttc tgtagtcgtt catcagctgg
     7621 caaagcctcc cagtctcttt ccaaggaaaa taactgatca caataagcta aacctttagc
     7681 tcctaaggat gatttatctg cttgcttggg ggtcgcttca aaaaatttcc ttctcacatg
     7741 cgcccaacat ccaacaagtg cagcttcttc cagttgacga taagcttcta gcggttcgca
     7801 gtttaccaag cttagcgtcg ccttgctcct aaaccttgga ctgctatcgc ataggcagaa
     7861 ctagaggact aaagtcctaa gttactgccg caacatatca caataaacat agccagaata
     7921 atctcctagg aattcttgta ctactgaacc acttcgacac tgatcatggt ggtaaagcgt
     7981 aatcccttgt ttctctgatt tacctgacaa aaaagtccaa tagtaagtca gctgactatc
     8041 actttctaag acccgataag aagtttcatc cgcatgaagt aagggctgag tcaatagtct
     8101 ctctcgcaag aggttataaa ggggctttag gaataggagc tttcacaagc ttatccagat
     8161 gattatcttt tactcgttat ggacaatgct atatggcata aatcaagtac cttaaagatt
     8221 ccgactaata ttggttttgc atttattcct ccatacacac cagagatgaa ccccattgaa
     8281 caagtgtgga aagagattcg taaacgtgga tttaagaata aagcctttcg aactttggaa
     8341 gatgtcatga atcaactcca agatgttata caaggactgg agaaggaggt gataaagtcc
     8401 atcgttaatc ggagatggac tagaatgctt tttgaaagca gatgagtata aaaagaaagt
     8461 cctcatttca atagaaatca cgactttctg atggatttat agtaaaatga aataagaaca
     8521 ggacaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaag cagaggtgta
     8581 ctattctagt ttcaatctac tatatttttg gagtgataga aaagcccttc atcagccaat
     8641 ctacttgttc aggtgcgaga gctttgacat ccttttctgt actgggccaa gtcagtctgc
     8701 cgttctcaaa gcgtttatat agtagccaaa atccttgacc atcccagtaa agggctttaa
     8761 agcggtcttt acgtccacca caaaagagaa aaacttgacc tgagaaagaa tccaattcaa
     8821 agtgggtttt aaccagataa gccagtgaat cgattccttg tctcatatcc gttttcccac
     8881 atacgagata gacctgccct aggctagata gatgaattgt catagagcaa caccttatcc
     8941 aaaagctgtt ctatcatttc gagattgaga gattgaaaaa aacttacttc aagcttgcca
     9001 atacgaattt taagtagaat atcatttctc tttttagaat caaaaggacg agattgtgga
     9061 atctctacag gaacaatagg ttgtagcatt aaaatatcct ccaatagttt tactttacta
     9121 atagtataca ggaggagctc catgaatgat agataccttg ttatgacgcg cttactaaac
     9181 aactaactga tgcacgattt aagcgccttg ttggtgttca gcgcacgact tttgaagaga
     9241 tgttagctgt attaaaaaca gcttatcaac ttaaacacgc aaaaggtgga cgaaaaccta
     9301 aattaagcct agaagacctt cttatggcca ctcttcaata tgtgcgagaa tatcgaactt
     9361 atgaagaaat tgcggctgat tttggtatcc acgaaagcaa cttaatccgt cggagccaat
     9421 gggttgaagt aactcttgtt caaagtggtg ttacgatttc aagaactcct ctcagctctg
     9481 aggacacggt aatgattgat gcgacggaag tacaaatcaa tcgccctaaa aaaagaatta
     9541 gcgaatcatt ctggtaaaaa gaaatttcac gctatgaagg ctcaagcgat tgtcacaagt
     9601 caaaggagaa ttgtttcttt ggatatcact gtgaactatt gtcatgatat gaagttgttc
     9661 aaaatgagtc gcagaaatat cggacaagct ggtaaaatct tggctgacag tggttatcaa
     9721 gggctcatga agatatatcc tcaagcacaa actccacgta aatccagcaa actcaagccg
     9781 ctaacagctg aagataaagc ctgtaatcat gcgctatcca aggagagaag caaggttgag
     9841 aacatctttg ccaaagtaaa aacgtttaaa atgttttcaa caacctatcg aaatcatcgt
     9901 aaacgcttcg gattacgaat gaatttgatt gctggtatta tcaatcatga actaggattc
     9961 tagttttgca ggaagtctat tgaaggatct tttgctttag ttccttggtc aaggttgatt
    10021 attttcacgg agcgcttccg attgttttca taaaccattt gcgcatagtc agcagaataa
    10081 atctttttga aacgtccctt tcctagacat tatagaactt tcagattcat ctgaatttac
    10141 caaattaaca caaaaattct gaaaattctg ttgacatctt tctgaaaaga gtttataatg
    10201 gagagaaagt tttaaaggag aaaatgatga aaagttcaaa actatttgcc cttgcgggcg
    10261 tgacattatt ggcggcgact actttagctg catgctctgg atcaggttca agcactaaag
    10321 gtgagaagac attctcatac atttatgaga cagaccctga taacctcaac tatttgacaa
    10381 ctgctaaggc tgcgacagca aatattacca gtaacgtggt tgatggtttg ctagaaaatg
    10441 atcgctacgg gaactttgtg ccgtctatgg ctgaggattg gtctgtatcc aaggatggat
    10501 tgacttacac ttatactatc cgtaaggatg caaaatggta tacttctgaa ggtgaagaat
    10561 acgcggcagt caaagctcaa gactttgtaa caggactaaa atatgctgct gataaaaaat
    10621 cagatgctct ttaccttgtt caagaatcaa tcaaagggtt ggatgcctat gtaaaagggg
    10681 aaatcaaaga tttctcacaa gtaggaatta aggctctgga tgaacagaca gttcagtaca
    10741 ctttgaacaa accagaaagc ttttggaatt ctaagacaac catgggtgtg cttgcgccag
    10801 ttaatgaaga gtttttgaat tcaaaagggg atgattttgc caaagctacg gatccaagta
    10861 gtctcttgta taacggtcct tatttgttga aatccattgt gaccaaatct tctgttgaat
    10921 ttgcgaaaaa tccgaactac tgggataagg acaatgtgca tattgacaaa gttaaattgt
    10981 cattctggga tggtcaagat accagcaaac ctgcagaaaa ctttaaagat ggtagcctta
    11041 cagcagctcg tctctatcca acaagtgcaa gtttcgcaga gcttgagaag agtatgaagg
    11101 acaatattgt ctatactcaa caagactcta ttacgtattt agttggtaca aatattgacc
    11161 gtcagtccta taaatacaca tctaagacca gcgacgaaca aaaggcatcg actaaaaagg
    11221 ctctcttaaa caaggatttc cgtcaggcta ttgcctttgg ttttgatcgt acagcctatg
    11281 cctctcagtt gaatggacaa actggagcaa gcaaaatctt acgtaatatc tttgttccac
    11341 caacatttgt tcaagcagat ggtaaaaact ttggcgatat ggtcaaagag aaattggtca
    11401 cttatgggga tgaatggaag gatgttaatc ttgcagattc tcaggatggt ctttacaatc
    11461 cagaaaaagc caaggctgaa tttgctaaag ctaaatcagc cttacaagca gaaggtgtga
    11521 ctttcccaat tcatttggat atgccagttg accaaacagc aactacaaaa gttcagcgcg
    11581 tccaatctat gaaacaatcc ttggaagcaa ctttaggagc tgataatgtc attattgata
    11641 ttcaacaact acaaaaagac gaagtaaaca atattacata ttttgctgaa aatgctgctg
    11701 gcgaagactg ggatttatca gataatgtcg gttggggtcc agactttgcc gatccatcaa
    11761 cctaccttga tattatcaaa ccttctgtag gagaaagtac taaaacatat ttagggtttg
    11821 actcagggga agataatgta gctgctaaaa aagtaggtct atatgactac gaaaaattgg
    11881 ttactgaggc tggtgatgag gctacagatg ttgctaaacg ctatgataaa tacgctgcag
    11941 cccaagcttg gttgacagat agtgctttga ttattccaac tacatctcgt acagggcgtc
    12001 caatcttgtc taagatggta ccatttacaa taccatttgc attgtcagga aataaaggta
    12061 caagtgaacc agtcttatat aaatacttgg aacttcaaga caaggcagtc actgtagatg
    12121 aataccaaaa agctcaggaa aaatggatga aagaaaaaga agagtctaat aaaaaggctc
    12181 aagaagatct cgcaaaacat gtgaaataac tgttgcaaaa tataagaaag gatttagtat
    12241 ttctcttgaa tgctgaatcc ttttttacat ttgtaaagaa agattctaaa tgtactgccc
    12301 cccaaaagtt ggagcctctt tttgtcagaa tagagaaaat ttttgttaat tttacttgtt
    12361 tcctattgct ttat
//
LOCUS       DAVKJD010000054        11587 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKJD010000054 DAVKJD010000000
VERSION     DAVKJD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.054"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            143..901
                     /locus_tag="VW157_001492"
     CDS             143..901
                     /locus_tag="VW157_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9552771.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LSPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            941..1705
                     /locus_tag="VW157_001493"
     CDS             941..1705
                     /locus_tag="VW157_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9552772.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNSKTIGVAQGSITQCLITELGKKKGLTFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1718..2395
                     /locus_tag="VW157_001494"
     CDS             1718..2395
                     /locus_tag="VW157_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9552773.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2405..3064
                     /locus_tag="VW157_001495"
     CDS             2405..3064
                     /locus_tag="VW157_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9552774.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3322..4512
                     /locus_tag="VW157_001496"
     CDS             3322..4512
                     /locus_tag="VW157_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9552775.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITYLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(4833..5714)
                     /locus_tag="VW157_001497"
     CDS             complement(4833..5714)
                     /locus_tag="VW157_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9552776.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5855..7183)
                     /gene="vncS"
                     /locus_tag="VW157_001498"
     CDS             complement(5855..7183)
                     /gene="vncS"
                     /locus_tag="VW157_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9552777.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(7180..7836)
                     /gene="vncR"
                     /locus_tag="VW157_001499"
     CDS             complement(7180..7836)
                     /gene="vncR"
                     /locus_tag="VW157_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9552778.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7885..7968)
                     /gene="pep27"
                     /locus_tag="VW157_001500"
     CDS             complement(7885..7968)
                     /gene="pep27"
                     /locus_tag="VW157_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9552779.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(8148..9527)
                     /gene="vex3"
                     /locus_tag="VW157_001501"
     CDS             complement(8148..9527)
                     /gene="vex3"
                     /locus_tag="VW157_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9552780.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9579..10226)
                     /gene="vex2"
                     /locus_tag="VW157_001502"
     CDS             complement(9579..10226)
                     /gene="vex2"
                     /locus_tag="VW157_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9552781.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIQ
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10239..11516)
                     /locus_tag="VW157_001503"
     CDS             complement(10239..11516)
                     /locus_tag="VW157_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552782.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQVSLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3270 a         2588 c         2185 g         3544 t
ORIGIN      
        1 attgttagaa atcgatttga ctgtcccgat cgatttttcc tgttcttatt tcactatagt
       61 gaattttttc agatatgatt tgacaagaac attcttttat tgcagaatcg tgttataaaa
      121 tctaacataa aggagttctc tcatggcttt agtagaattt aaaaacgtcg aaaaatatta
      181 cggagactac cacgcactcc gcaacatcaa tctccgtttt gaaaaaggac aagttgttgt
      241 cctgcttgga ccttctggct ctgggaagtc cactcttatc cgtacgatca atggtttaga
      301 ggctgttgac aaaggaagtc tcctagtcaa tgggcaccaa gttgctggtg ccagccagaa
      361 agatttggta cctcttcgca aggaagtcgg catggttttt caacatttta acctttatcc
      421 acacaaaaca gtgttagaaa acgtgacact ttcacccatt aaagttctag gaattgataa
      481 aaaagaagct gaaaaaaccg ccaaaaaata tctggaattt gtaaatatgt gggacaagaa
      541 agattcctat cccgccatgc tatctggtgg acaaaaacag cggatcgcca tcgctcgtgg
      601 tcttgctatg catccggaac tcctcctctt tgatgaacca acatctgctc ttgatcctga
      661 gactatcgga gatgttctag cagttatgca gaaactggcg cgtgatggga tgaacatgat
      721 catcgttacc cacgaaatgg gctttgctcg agaggttgcg gaccgcatta tctttatggc
      781 cgacggagaa gttttagtag atacgacaga tgtcgataac ttttttgaca atccaagcga
      841 acctcgtgcc caacaattcc tcagcaaaat tatcaaccac gaaagtgaca aagtcaaata
      901 aggaggcgcc tatgaaaaaa aattcttttt atcagcatta ttgattagcc ttttcggcct
      961 tgctgctgcc aaaccagtcc aggctgatac aagtatcgca gacattcaaa aaagaggcga
     1021 actggttgtc ggtgtcaaac aagacgttcc caattttggt tacaaagatc ccaagaccgg
     1081 tacttattct ggtatcgaaa ccgacttggc caagatggta gctgatgaac tcaaggtcaa
     1141 gattcgctat gtgccggtta cagcacaaac ccgcggcccc cttctagaca atgaacaggt
     1201 cgatatggat atcgcgacct ttaccatcac ggacgaacgc aaaaaactct acaactttac
     1261 cagtccctac tacacagacg cttctggatt tttggtcaat aaatctgcca aaatcaaaaa
     1321 gattgaggac ctaaacagca aaaccatcgg agtcgcccaa ggctccatca cccaatgcct
     1381 gattactgaa ctgggcaaaa agaaaggtct aacctttaaa tttgtcgaac ttggttccta
     1441 cccagaattg attacttccc ttcacgctca tcgtatcgat gccttttccg ttgaccgctc
     1501 tattctgtct ggctacacta gtaaacgcac agaactacta gatgatagtt tcaagccatc
     1561 tgactacggt attgttacca agaaatcaaa tacagagctc aacgactatc ttgataactt
     1621 ggttactaaa tggagcaagg atggtagttt gcagaaactt tatgaccgtt acaagctcaa
     1681 accatctagc catactgcag attaaggagg acaccccatg acagatttat catcttggac
     1741 agcctatttt caggattttg gacaattttt caatggtttc ctctttactc ttgccctagc
     1801 gattggatcc tttatcctcg ccatggtctt aggaatcttc tttggtgcca tgtcaaccag
     1861 taaacgtcca attttacgca ttttagctca catctttgtg gaattttacc aaaacactcc
     1921 cctcttggtg cagtttgtta ttgtttttta tggcctacct cttatcagtg accatactat
     1981 catgattcca atttattgga cagctgttct ctgcgtggga ctctatcacg gcgcttatat
     2041 cgctgaggtt attcgttcag ggattcagtc tattcctagt ggtcagatgg aggccgcctt
     2101 gtcgcaaggt tttacctata tcagtgccat gcgcttgatt atcttgcctc agaccttccg
     2161 catcattctc cctccattga ccaaccaaat tgttaacctc atcaagaaca cctctactgt
     2221 agctatcatc tctggagtag acttgatgtt tgtgactaag tcttggtcgg ctctcaacgg
     2281 aaactatatc ccagcctttt taggtgctgc tcttctctac tttgccctat gcttccctgt
     2341 tgcccagttt ggtcgcaaga tggagcaagc caacaaaaaa gcctattcac tttaggaggt
     2401 tactatggaa tctattttag aagttttaac cccagataac ctagtcttta tctttaaagg
     2461 atttggcttg accctctata tttctctgat tgccatcatc ctctctacta tcatcggtac
     2521 ggtgctagct gtcatgagaa atggcaaaaa tcctgtctta cgcattattt ccagtattta
     2581 tatcgagttt gtgcgcaacg ttcccaacct tctctggatt tttactatct ttttggtgtt
     2641 caaaatgaaa tccacaccag caggtattac agcctttact ctctttacat cagcagcctt
     2701 ggctgagatt attcgaggcg gtctcaatgc cgtagacaag ggacagtacg aagcaggaat
     2761 gtcacaaggc ttcacctcag cccaaatcct ctactacatc attctcccac aagccatccg
     2821 caaaatgcta ccagccatca tttctcagtt tgttaccgtg attaaggata ccagtctcct
     2881 ctactctgtt atcgccctac aagaactctt tggagccagc caaattctca tgggccgtta
     2941 tttcgaacca gagcaggtct tcagtcttta catcctgatt gccctcatct acttcagctt
     3001 taacctagca atttctagcc tgtctcatat gctagccaaa cgttggcaac aagctgcaga
     3061 ataatactct tcgaaaatct cttcaaacca cgttagctct atctgcaacc tcaaaacagt
     3121 gttttgagca acttgcggct agcttcctag tttgctcttt gattttcatt gagtataaac
     3181 agccactctc cagttcaatt ggagagtttt ttgatgagag tagatattta tagacctcaa
     3241 tttgacacca tacattttct atgataaaat gtaacaaatt tatttacaac aaatcgaaat
     3301 ctaaatgaaa tggaattttc tatgaaatca gtcaaaggac tactctttat catagctagt
     3361 tttatcttga ctcttttgac ttggatgaac acttctcccc aattcatgat tccaggacta
     3421 gctttaacaa gcctatctct gacttttatc ctagccactc gtctcccact actagaaagc
     3481 tggtttcacg gtttggagaa ggtctacacc gtccacaaat tcacagcctt tctctccatc
     3541 atcctactaa tctttcataa ctttagtatg ggcggtttgt ggggctctcg cttagctgct
     3601 cagtttggca atcttgccat ctatatcttt gccagcatca tccttgtcgc ctatttaggc
     3661 aaatacatcc aatacgaagc ttggcgatgg attcaccgcc tggtttacct agcctatatt
     3721 ttaggactct ttcacatcta catgataatg ggcaatcgtc tccttacatt taatcttcta
     3781 agttttcttg ttggtagcta tgccctttta ggcttactag ctggttttta tatcattttt
     3841 ctatatcaaa agatttcctt cccctatcta gggaaaatta cctatctcaa acgcttaaat
     3901 cacgatacta gagaaattca aatccatctt agcagacctt tcaactatca atcaggacaa
     3961 tttgcctttc taaagatttt ccaagaaggc tttgaaagtg ctccgcatcc cttttctatc
     4021 tcaggaggtc atggtcaaac tctttacttt actgttaaaa attcaggcga ccataccaag
     4081 aatatctatg ataatcttca agccggcagc aaagtaaccc tagacagagc ttacggacac
     4141 atgatcatag aagaaggacg agaaaatcag gtttggattg ctggaggtat tgggatcacc
     4201 cccttcatct cttacatccg tgaacatcct attttagata aacaggttca cttctactat
     4261 agcttccgtg gagatgaaaa tgcagtctac ctagatttac tccgtaacta tgctcagaaa
     4321 aatcctaatt ttgaactcca tctaatcgac agtacgaaag acggctatct taattttgaa
     4381 caaaaagaag tgcccgaaca tgcaaccgtc tatatgtgtg gtcctatttc tatgatgaag
     4441 gcacttgcca aacagattaa gaaacaaaat ccaaaaacag agcttattta cgaaggattc
     4501 aagttcaaat aagtaatcat actctcctgg gatttcccag gagagttttt tatagccaaa
     4561 tgaaataaga caggaacgat ggaattttgg cattcaaatc aattgataag aatgttttat
     4621 agtggagtga aataggatac gaacaaattg attaggaaaa tcaaatgaat ttatagaaat
     4681 cttttagcag ttatgttatc ctattctagt ttcaaaacgc tatagaagca gcattgtgct
     4741 agtctagatt cagtttacta tactaaaacg agtagcttga aatcaaaaaa cccaccctca
     4801 caggcaggtt ttatctgtat tattcagcta gattatgctt taccttctga accgaatacg
     4861 tcgatacgtt cttcaaccga tgcttggata gcttttacac cgtcagccaa gaatttacgt
     4921 gggtcgaaga gttttttctt gtcgtattct gcttcgtttg cttcgtagtc acgagcaaat
     4981 ttacgagttg cgttagcgaa tgcgatttgg cattctgtgt taacgttaac tttggcaaca
     5041 ccaagtttga tagctgcttg gatttgctca tcaggaatac ctgatccacc gtgcaatacg
     5101 attgggaatc ctggaagagc ttctgtcaat ttttgcaagt ggtcaaggtc aagaccttcc
     5161 cagtttactg ggtaaggacc gtggatgtta ccgataccag ctgccaagaa gtcgatacca
     5221 gtttcaacca ttgctttagc gtcttcgatt ggagccaatt cacctttacc gatgattccg
     5281 tcttcttcac caccgatagt accaacttca gcttctactg agataccttt agcgtgtgct
     5341 ttttcaacaa cttctttagc caatttaagg ttttcttcaa ctggaaggtg tgaaccgtca
     5401 aacatgattg aagtataacc aacttcgata cactcaagtg catcttcgta gtgaccgtgg
     5461 tcaaggtgga tagctactgg tacagtgata cccattgatt caacaaggtt agcgatcaag
     5521 ttgcgagcaa ctttgtaacc acccatgtat ttagcagcac ccattgaagt ttggatcaaa
     5581 actggagctt ttttagcttc tgctgcgcgc aagatagctt gagtccactc aaggttgttt
     5641 gtgttaaatc caccaactgc ataaccgttg tcacgggctg cttggacaaa tttttctgct
     5701 gaaacgattg ccattttatc aggcctcctg tatattttta tgggtcatcc catttacatt
     5761 gttcatttta tcactttttg ccaaaaaaat ctagtttttc ccgcagtttc gattgatttt
     5821 cttctaactc catctatgta aaccctttct ctccctagtc ttggacaact tttggaaaat
     5881 ctataaagaa ggttaaacga ttctcctcca tctcgaaacg ataagctaat ttttcatgtt
     5941 ctaatagact cttaaccaca aagagcccca taccagaccc cttgaccttg cgactagcat
     6001 tgtcagaaaa agactgggct agtttttctt gttcctctga gctacagcta ttttcgataa
     6061 aaagttctcc ttctctttct ccaattcgaa ctaagccacc tggaacagag tgcttaatgg
     6121 cattgctgat gagattagaa agaatcaact tcataactga tgggtttaga taagcctgct
     6181 gatgggtcaa actattgtct atctggagct ctctttcctt ggctagcaag gcataatctt
     6241 tgaccagatt ttgcgtcatc tggaggaggt caattgtttc cctatcatct cgcaattcct
     6301 gcacagaaga gagggaaagt atctgcagaa catggtgatt gagttcatcc acaatcccca
     6361 aggcaactcc cagatactgg tctctatcct tataacgacc gatattctct ctcatatttt
     6421 cgattaggat tttcaaacta gccagcggtg ttttcaattc atgagaagct cctcgtagga
     6481 attcgacctt catcttctcc agctggagaa tggcttcatt cttttcatgc aagtccgcaa
     6541 taacagtcaa gagatgctgg tagaggctat tgatttgttc cttgagatta cctatctcat
     6601 ccttagaatc cacgcgcaat cgcacttggg aatccaggtc catcatccga cgggtcaccc
     6661 gcttgatttc caaaatcggt gcaacaatag tccgagcgta gatgtaggcc accaagaggg
     6721 aaatcagaaa ggaggccagc aaggtatagg gaagaaactg gagactgatt tgctctgctt
     6781 ccttttgtaa atccatggaa gctagaaact ggagaatcat agtaccaccg tcttgcgttt
     6841 tcacctcgcg ctcctcaata aagagagagg ttgtctggcg gtctgtgtcc agaggaagac
     6901 tgtccttgac ttctaacttg tcctcggtca tctcaccttt gacggtcccc ttgatatcac
     6961 tagtctggga atacaagtct aacacttgct cgatactctg cctatctttc ccttctaggg
     7021 actgggcaat ggctgttgcc ttttgaccaa tggtttcctg acgatgactc agataagtcg
     7081 aaggaaaaag aaaataaata gctaaatgaa ggcagataac cagaacacta aatatcgaga
     7141 aggtatagat aaatatcttt gcaaataaac ctgttcgttt cattttcgct ccaatttata
     7201 accaacattg cgcacagtga ggatacaatc caagtctagc tttttccgca attccttgat
     7261 ataaacatca ataacacggt caaagggaac ctcatctgtc gctttccaga cggcatcgat
     7321 aatctgagac cgagtcaagg cccggccttc atttttcact agatagtcca gaatttccaa
     7381 ctctttggca ttgataggca cttcttgacc tgcgaggctt gcactgtagc tttcaaagtc
     7441 caccttggta tccttgtaag aaaagattcg tcctgtatcg tagtagcgct tgaaaatcgc
     7501 gtccaccctc acttttaaga gggagaggga gaaaggtttt tccagatagc catctgccaa
     7561 agaggcaaag gcactcatct tgtattcctc atcctgaaaa gctgtcaaca tcaagacagg
     7621 aacctgactg gttttacgaa tctcagctag aacttctaag ccgttaagct tgggcatctg
     7681 gatatccagt aaaaccaggg ccacctcata gctagaaaat tgctccagag cttcctgacc
     7741 gtccgctgcc tcaatagttt catagccaca atccgtcaaa taatcactga ccccctcacg
     7801 gatcatctct tcatcttcta caattaaaat tttcatactt taactgctct ctatttttta
     7861 tttttcttag aataaatacc tactctattt tctattatag tctcttgctg gccttttgtc
     7921 tgtaagtaac tgatcactag ataaaacgtt gtgaaattcc tttctcataa attccatagc
     7981 tttagtatat tatatttaag caataaagta caaagaaaac aactgaaagc aatgattttc
     8041 accactgctt tcggatttat tttgaattgt taaatagcca ttcctatcca ctattcttga
     8101 atagaaacac aagatgcaat ctttattcca gactcatttt ttcaaattta ttcaccatcc
     8161 agcaagagct cttttggttg ttttctaagg agattgcttg aagcaagcgc cataacgaga
     8221 accactagaa ccaaggcaag gacaaaaatg atgataaagt ctgatgtctg aatagaaatg
     8281 tctaggctcg acaaggtctt gctaaagcca tctacttctg caccaccacc aaggttagag
     8341 gcttgagccg ccttactagc ctgtttggca acacctgaag tcacattggc aaggacagtg
     8401 tttccaattg cacgggcagt gtaattagct aggaagtaag cagaaactag agcagggata
     8461 gcaatcaaga tagattcggt gatgaattga cccaagatac ttgcctgctt gagaccaata
     8521 gagaggagga ttcccacttc cttgcgacgg gcattgatcc aaagactgag caagagggca
     8581 aggagaagga ctgagaagct caagctaccc cagaagagga ggttggccat cttgtacata
     8641 ccagagatag attgctcaag agctgggtag ttagaggagc tcttgacgag tgtgtagctc
     8701 ttccagttga taccactgat gccattcaac tctttcataa catcatccaa gttcttgtct
     8761 gctgttacaa agaaggttgc gtccccataa atggctgtgt cttctgtgta tccataaagt
     8821 tttgcagcag tgtgaatgtc tgtaatagct gtgttttcgt aaagttcttg tgagtaggtt
     8881 actgctgact tattatgacc atcaaagagt cccttgattg tcacttcaac tgtttccttg
     8941 gctccttttt cattatctgc atcgtagata ttagagtcca gtttaacctt gtcccctact
     9001 ttccagccgt gtttggctgc caagtccttg tgcaagagga ttttatcctt gtcgtcgttg
     9061 gttaagtgct ctccttcgac tagtttataa gaaccagaga caaacttgtc ttctttagag
     9121 gagtcattga cacctgtaat catcaagcta cttccaaaac gtttggcacg atcagcagtg
     9181 agattcttct tggtttctgg cgtttcaatc aggtcatatc cagtcaaatc tccgatagcg
     9241 ttgatacgtt tgacataaga ctcaatggcc ttgttttcgg tgattttttt gatgtcttca
     9301 cccttgatat tcccagcacc acgaggcgtt ccttggttga cgcgacgatt gatttgcatg
     9361 gagaagctat tggtgatatt tttaaaggtc tcctgagaag ccttggcagt agctcccttg
     9421 attgacaagc cgaccaaact caagctcgcc ataaggagaa taatcaggaa gatgacaatc
     9481 gatttgaaaa acttccttgt aacataggca aatgcgttgt gtaacataga ttccctttct
     9541 agattttgtt ttaatcattc tatcaaaata agctcaaatt atttactagt attgcgcgtt
     9601 tcagtcagtt tcttatcctt taattcaagt gtaatatctg acgcttgtgc cacttcttta
     9661 ctgtgagtta cgacaatcac acatttacct gttttctggg caagtgattt gagtagttcg
     9721 acaatatctc cagcagtttt aggatccaga tttcctgttg gctcatcagc tagaataact
     9781 ggagcttctg agaccaaact gcgagcaatg gcaacacgtt gctgttgacc acctgataac
     9841 tggagaacat tccgcttgat ctggctttca tccaaaccaa gctcaagaag tgtattcttg
     9901 cttgcctttt tgttgaccaa ttggatattt tccagcggag aaagataatc tatcaagtta
     9961 taattttgaa agaccaggga aatatggtgc atgcgatggt aagaatagcc cttcttacga
    10021 atatcctctc cttgaaaaag gatagaacct tcaacaggac tatctagacc agcaagtagg
    10081 gacaagagtg tggattttcc tgctcctgac tccccaataa tactgtaaaa ttttccgggt
    10141 tcaaaattat aattgatctg atataggact gcttcagcag tattcttata acggtaggta
    10201 acatcttgta attgtaataa agtcatgatt tctccttctt aactaataga tgataaaatt
    10261 tctttcggtg attttctaaa taagaatagg aaacaaaggg ctacagataa gcaactaagc
    10321 agaactagaa aaacatagga ttctgcaaaa gataagatgc tagttgataa actgcttgct
    10381 ttggctagtg tatcttgtaa gcttacctga tctccacttg ctagtagagt ttggagtagg
    10441 taagttgtga ttccgtttcc tgcaacaaat gctggaagca aagctccaag agataccaaa
    10501 actacctcta aacagaattg taggaagatc gagctcttgc cttttccaag tgcaagtaaa
    10561 atccccactt catagacccg ttctctcaac cagagagaca aaaccagaat taaggctcca
    10621 gctcctgcta tcaacatccc ataaaggaag atggtcagga aggtttggaa agttgcaact
    10681 gagtctttga tttgttcaaa agccttgttt tccttttcga cttggtagcc ttgattttcc
    10741 aaggccaagt tttctacctg cttcatgagt ccgtccattt ccttaggatt ttctacatag
    10801 aagcgtgctg cactgacttg agcttcacta ttgcccaaaa gggtttggct actttcataa
    10861 tctgtaaaga cttgattttc actgaagtca gaagacaagc ctgtgaattt ctcttgtttt
    10921 ttaccagaaa agatgccgat aatctcaaac tctactgttt gtccttttcc agattcagac
    10981 tgaccagcat ccaagccaat cttgtcatga agtgaaagac cgttcttctt agccaattct
    11041 tcgtggataa ggattttctt ggaatcccct ttttgaaggt gtcgcccttc ttttagattg
    11101 aaagccgaac tggtaaaggt tacatccttg gatgaatcct caagagccgt taagctaacc
    11161 aagttattgt ctgcagctga taaatcatca cgctccacgc tctgctcgcc agtcactgct
    11221 tccttgtctt ttagttttgc gaccgtctca agttcaggag agacattttc cagcccctta
    11281 atcttgctta cagatgctag gtctgacaac ttgaatgtct gaccattctc tatcttctta
    11341 atagaaaaag atgtattgag tgatttataa agattgcttt ctactgtttt gttggacttc
    11401 atcagagtca aacaggctga aattccggcc aataagacca ataaaatcag aaataaaata
    11461 aaacttctca gtcgctttct gctgacataa gcccaagatc tttggattgg attcatttgt
    11521 cacctccata tttgtaagac tattataaaa cccaaatatg aaatgtttat gaaatacgaa
    11581 aaaaaaa
//
LOCUS       DAVKJD010000055        11525 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKJD010000055 DAVKJD010000000
VERSION     DAVKJD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11525
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.055"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            112..765
                     /locus_tag="VW157_001504"
     CDS             112..765
                     /locus_tag="VW157_001504"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9552783.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTSWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            775..1239
                     /locus_tag="VW157_001505"
     CDS             775..1239
                     /locus_tag="VW157_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9552784.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1252..2520
                     /locus_tag="VW157_001506"
     CDS             1252..2520
                     /locus_tag="VW157_001506"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9552785.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAAGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALFLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2534..2794
                     /locus_tag="VW157_001507"
     CDS             2534..2794
                     /locus_tag="VW157_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552786.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2805..4166
                     /gene="pdrM"
                     /locus_tag="VW157_001508"
     CDS             2805..4166
                     /gene="pdrM"
                     /locus_tag="VW157_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9552787.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4320..5288
                     /locus_tag="VW157_001509"
     CDS             4320..5288
                     /locus_tag="VW157_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9552788.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5304..6296
                     /locus_tag="VW157_001510"
     CDS             5304..6296
                     /locus_tag="VW157_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9552789.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6514..7557
                     /locus_tag="VW157_001511"
     CDS             6514..7557
                     /locus_tag="VW157_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9552790.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7621..9306
                     /gene="lpdA"
                     /locus_tag="VW157_001512"
     CDS             7621..9306
                     /gene="lpdA"
                     /locus_tag="VW157_001512"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9552791.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHSAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9370..10359
                     /locus_tag="VW157_001513"
     CDS             9370..10359
                     /locus_tag="VW157_001513"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9552792.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRETGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEAVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10432..11502)
                     /gene="xerS"
                     /locus_tag="VW157_001514"
     CDS             complement(10432..11502)
                     /gene="xerS"
                     /locus_tag="VW157_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9552793.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         3386 a         2170 c         2618 g         3351 t
ORIGIN      
        1 attcaaatcg tatatatagt cgatttatta aatctttaga taaactgtaa aaagtggttt
       61 ccatagccac tttttgctat aatagaggca gtaaaaatga aggagtcggc tatggaacac
      121 tcgtcttggc atgctttgat taaggcgcaa ttacctgagg gttatttcgg gaaaatcaat
      181 cagtttatgg agcaggtcta ttctcagggg attatttatc cacccaagga aaaggttttt
      241 caggctctct tgacaacact gcttgaagaa gttaaggtgg taattctagg gcaagacccc
      301 tatcacggac caggtcaagc gcagggcttg agtttttctg tacctgactc tatcccagct
      361 ccaccatcct tgcaaaatat cttgaaagaa ttgtcagatg atatcggagt taagaaatct
      421 catgatttga catcttgggc tgagcaagga gtcttgcttc ttaatgcttg tttgactgtt
      481 cctgctggac aggccaatgg tcatgctggt caaatctggg agccttttac tgatgctgtg
      541 attcaggtgg tcaatcatct agatagacca gtcgtttttg tactctgggg agcttatgca
      601 cgtaagaaga aggcattagt taccaatcct catcacttga ttatcgaatc agcccatcca
      661 agtcctttgt cggtttatag aggattttgg ggttccaagc ccttttccaa ggccaatgca
      721 ttcttaaaag agacaggaca agagccaatc gattggctta gataaggaga aaatatgcct
      781 cagttagcga cgatttgcta cattgataat gggaaagaac tgctcatgct ccatcgtaat
      841 aagaaaccca atgatgtcca tgaagggaaa tggattggtg tgggtggtaa gctagagaga
      901 ggagagacgc cccaggaatg cgcggcgcgt gaaatccttg aagaaacagg gctcaaagcc
      961 aagccagttc taaaaggtgt catcactttt cctgaattta caccagattt agactggtac
     1021 acctatgttt ttaaggtgac ggagtttgag ggcgacttga ttgactgcaa tgaggggacg
     1081 ctagaatggg ttccctatga tgaggttttg agcaagccga cttgggaagg tgaccacacc
     1141 tttgttgagt ggcttttaga ggataaaccc ttcttttcag ccaagtttgt ttatgatggg
     1201 gataaattgt tggataccca agttgatttc tatgaataaa ggagaaagca gatgctacta
     1261 atcaaaaatg gtcgtgtaat ggatcccaag tctggtttgg atcaagtttg tgatgtctta
     1321 gttcaagatg ggaaaattat caaaattgcg cctgagatca cggaagaagg agcagaaacg
     1381 attgatgcta ctggtcttgt ggttgctcct ggtttggtcg atattcatgt gcatttccgt
     1441 gaacctggtc aaacacataa agaagacatt catactggtg ccctggcagc cgctgcaggt
     1501 ggttttacta ctgtcgtcat gatggctaat accagtccaa ccatttcaga cgtggagact
     1561 ttgcaagcag ttctccagtc agctgccaaa gagaagatta atgtcaagac agttgcgacc
     1621 attactaaaa actttaatgg taaaaacttg actgacttta aggcgctctt agaagctggt
     1681 gcggctggtt tctctgatga cggtattccg cttgagagca gtaagattgt caaggaagcc
     1741 atggaggaag ccaaaaaact caataccttt atcagccttc atgaggaaga tccaggtttg
     1801 aacggtgttc ttggctttaa tgaaaatatt gctagagaac atttccatat ctgcggtgct
     1861 actggggtgg ctgagtacgc tatgatggcg cgtgatgtca tgattgccta tgcaactaaa
     1921 gcccatgttc acatccagca tttgtctaag gaagaaagtg ttaaagtagt ggagtttgct
     1981 caggggttag gtgcagaagt cacagcagaa gtagcgccac agcatttctc taagacagaa
     2041 gcactttttt taacacaagg tagcaatgct aagatgaatc caccgcttcg tttggaatca
     2101 gaccgtcgtg ccgttatcga aggtctcaaa tcaggtgtca tcacagttat tgcgactgac
     2161 cacgcgcctc atcatgtaga tgaaaaaaat gttgaggata ttaccaaagc gccatctggt
     2221 atgactggct tagaaacatc cctgtctctc agcttgacct atttagtaga agctggtgag
     2281 ttgagcttga tggaattact tgaaaaaatg acatacaacc cagccaagct ttacaacttt
     2341 gaagcaggtt acttggctga gaatggtcca gcagatatca ctatttttga tgccaaggct
     2401 gaccgccttg tggactccca ttttgcttcc aaagcagcta attcaccatt catcggtgaa
     2461 accttaaaag ggcaggttaa atataccatc tgtaagggac aaatcgtcta tcaagcttga
     2521 taggagttag aatatgaatt attttcgaaa acgtagggag agacaagcga aatctaatag
     2581 tggaatttac tgtcccgcag ctaatcgttc caaaattcaa tatgaaacta aagaaaaagc
     2641 tgaccatgcg gttcaatatg ataacggagg tttagttaga agctattatt gtcgtacatg
     2701 tgcgtgttgg catactactt caaaatctaa taaacctccg ctaagtttga aaaactatgg
     2761 tctgaaactc tttggtttag ataaatcaga atagaaagag aagtatgtat aagacaaagt
     2821 gtttacgaga gaagttagta ttatttttaa aaattttctt cccaatcctg atttaccaat
     2881 ttgccaatta ttctgcctct tttgttgata ctgcaatgac aggtcaatac aacactatgg
     2941 acttggctgg tgtatctatg gcaaccagta tctggaatcc tttctttaca tttctaacag
     3001 ggattgtgtc agccttggtg cctatcattg gtcaccatct tggtcgagga aaaaaggaag
     3061 aagttgcgtc tgatttttac caatttattt atttggcctt gggcctatct gtggtattgc
     3121 tggggatggt acttttcttg gcaccaacaa tcttgaatca tattgggtta gaagcagcag
     3181 tagcggcagt agcggttcgc tatctttggt ttttatctat cgggattatc cccttgttgc
     3241 tctttagcgt cattcgttcc ttgctggatt cgctgggctt gaccaaactg tccatgtacc
     3301 tcatgctttt gttactccct ctcaatagcg gatttaacta tctcttgatt tacggtgcct
     3361 ttggtgttcc agaactggga ggggctggtg ctggtttagg aacatccttg gcctactggg
     3421 tcttgcttgg gatttctgtt ctggttttat ttaaacagga gaagctcaaa gccttacacc
     3481 ttgagaaacg aattccactt aatatggata aaattaagga aggagttcgt ttaggtctgc
     3541 ctattggggg aactgtcttc gcggaagtgg ctatcttttc agtggttggc ttgattatgg
     3601 ctaagttttc gtccttgatt atagctagtc accagtcagc tatgaacttt tcaagtctta
     3661 tgtacgcctt tcctatgagt atctcatcgg ctatggctat tgtcgtttcc tatgaagtgg
     3721 gagccaagcg atttgatgat gcgaaaacct atattggtct aggaagatgg actgccctca
     3781 tttttgcggc cttcacctta accttccttt acatttttag gggaaatgtg gccagtcttt
     3841 atggtaacga cccaaaattt atcgatttga cagtgcgttt tttaacttat agtcttttct
     3901 tccagttagc agataccttt gcggcgccgc ttcagggaat tttgcggggg tataaggata
     3961 cagttattcc tttttacctt ggtttgcttg gttattgggg cgtagcaatc cctgtggcta
     4021 cgctatttga ttctctaaca gattttggag cttattctta ctggatcggc ttgattatta
     4081 gtttgattgt gagtggggcg ctctaccgtt ggcgtttaac tgtgattatg aagagatttg
     4141 aatctttagc aaaatccaaa cgctaaaaag tttgtgaaaa aatattcttg ataagaaaat
     4201 cccttgtttt cacggggttt tcttttttat tttgtgaaat ttcttttaga aaaactttga
     4261 cagtatttgc caaaaacggt aaaatagtaa ggtaagaaga aagttagaaa ggcaagaaga
     4321 tgtcaacttt agataaaaat cttttgctag aaatgttccg taagatggaa gaaatccgtc
     4381 gcatggactt aaaaattgca caattagtaa agaaagggaa agtgccagga atgacgcact
     4441 tttctgttgg tgaagaggca gctaacgtgg gggctatgtt agctctcaat ccagatgatc
     4501 tgattacctc aaaccaccgt ggacacgggc aagctattgc taaagggatt gacctcaacg
     4561 gaatgatggc tgaaatcctt ggtaaataca ctggaacctg taaagggaaa ggtggatcta
     4621 tgcatatcgc tgaccttgat gctgggaacc ttggtgccaa tggtattgta ggtggtggta
     4681 tggggatcgc tgtcggtgca gccctcagtc agcaaatgca aaataccggt aaaatcgttg
     4741 tctgcttctt tggagatggt gcgaccaatg aaggtgtttt ccacgaagca gtgaacatgg
     4801 cttctatctg gaacctgcca gtcattttct attgcattaa caacggttac ggtatctctg
     4861 cggatatcaa gaaaatgacc aatatagaac atatccatca acgtagcgcc gcttatggaa
     4921 ttcctggaat gttcatcgaa gatggtaaca atgtcatcga tgtctatgaa ggatttcaga
     4981 aagctgtgga ccatgttcgc agtggcaatg gtccagtctt gattgaaagt gtaacttatc
     5041 gctggctcgg tcactcatca tctgaccctg gtaaatatcg tacgcgtgaa gaagtggaat
     5101 tgtggaaaca aaaagatcca atcgaaaacc tccgcaatta ccttattgaa aataacattg
     5161 caagtgccga agaattggaa gaaatccaag cgcaagtaaa ggaagcagta gaagcttctg
     5221 ttaaatttgc agaggaaagc ccattcccac cgcttgaatc agcctttgaa gatatttacg
     5281 cagactaagg agaaagagat aaaatggaaa caaaaacaat gtccttccgt gacaccatta
     5341 tccttgctat gtctgaggaa atgcgtcgcg atgaaaatgt gttcttgatg ggagaagacg
     5401 tcggtgtctt cggaggagac ttcggtactt ctgttggaat gcttgaagaa tttggtccag
     5461 aacgcgtccg tgactgtccg atttctgaag ctgccatctc tggagcagca gcaggagcag
     5521 ccatgacagg acttcgtcca atcgtcgata tgaccttcat ggacttctct gttattgcca
     5581 tggacaatat cgtcaaccaa gctgctaaaa cacgttacat gtttggtggt aaaggtcagg
     5641 ttccaatgac tgttcgttgt gcagctggta acggagttgg ctctgccgcc cagcactcac
     5701 aatctctaga gtcttggttt actcacattc caggacttaa ggttgtggca cctggaacac
     5761 ctgcggacat gaaaggcctg cttaagtctt ctatccgtga taaaaaccca gttattatac
     5821 ttgagtacaa gtcagaattt aaccaaaaag gggaagtgcc agttgatcca gactacacaa
     5881 ttccacttgg ggttggtgaa atcaaacgcc aaggaacgga tgtaacagtt gttacttatg
     5941 gaaaaatgct tcgccgtgtg gttcaagctg ctgaagaatt agcagaagaa ggaatttcag
     6001 ttgaaattgt tgacccacgt acccttgttc cgcttgataa ggatatcatc ataaactcag
     6061 tgaagaagac tggtaaggtt gttctggtca acgatgccca caaaacaagt ggctatatcg
     6121 gtgaaatttc agctattatt tcagaatcag aagcatttga ctatctagat gcaccaattc
     6181 gccgttgtgc aggagaagat gtgccgatgc cttatgcaca aaacctagaa aatgcaatga
     6241 ttccaacagt tgaaagtatc aaagatgcaa tccgaaaaac ttataacaaa gaatagagtt
     6301 gcataagata agttatgtaa ataaaataaa gctgacgaga aactttgtat cttttaggtg
     6361 cagagctctt cgtcattaat atcttagatt agagcacggg ctaaaagctc ggaaaaaaga
     6421 taaatctcct tggcttcatc gaagccatcg tcgatttcct atttttcagt cgcttttgac
     6481 gccctttgta tcataaatag aattggagag gtcatggctg atgacaagct aagagcgact
     6541 cctgcagcta gaaagttagc ggatgattta gggataaacc tctacgacgt ttctggctca
     6601 ggtgcaaacg gtcgtgtcca caaagaagac gtggaaactt ataaagacac aaacgtggtt
     6661 cgcatttcgc cacttgcaaa acgaattgcc ctcgaacata acattgcttg gcaggaaatc
     6721 caaggaaccg gtcatcgtgg taaaatcatg aagaaggatg ttttggccct gcttcctgaa
     6781 aatatcgaaa acgatagcat caaatctcct gctcagattg aaaaagtgga agaagtccct
     6841 gataacgtaa caccatatgg tgaaatcgag cgtattccaa tgacaccaat gcgtaaggtt
     6901 attgcccaac gcatggttga atcttacttg actgcgccaa ccttcaccct caactatgaa
     6961 gttgatatga ctgaaatgtt ggctcttcgt aagaaggttc ttgagccaat catggaagca
     7021 actgggaaga agactactgt aacagacctt ctttcgcttg cagttgttaa gactcttatg
     7081 aaacacccat acatcaacgc ttcattgaca gaagatggca agactattat cactcacaac
     7141 tatgtcaatc ttgccatggc agttgggatg gataatggat tgatgacacc tgttgtttac
     7201 aatgctgaga agatgagtct ttcagaactg gtggtagcct ttaaggatgt tattggccgt
     7261 accttggatg gtaaattggc tccaagtgag ttacaaaatt caacattcac aatcagtaat
     7321 ttgggaatgt ttggtgttca gtcctttggt ccgattatta accaacccaa ctcagctatc
     7381 cttggtgtca gttcgacaat cgagaagcca gttgtcgtca atggtgaaat tgtgattcgc
     7441 ccaatcatga gtttaggatt aaccattgac caccgtgtcg tagatggtat ggctggtgct
     7501 aagtttatga aggacttgaa agagttgatt gaaacgccaa tctcaatgtt aatttaagag
     7561 caagagtatt catttttaag atatagataa aggaaggaag acatggcctt agaagtaatt
     7621 atgccaaaag ccggcgtgga tatgacagaa ggacaaatcg tccaatggaa taaaaaagtc
     7681 ggagaatttg taaaagaagg agaaatcctt ttggaaatca tgactgataa agtcagcatg
     7741 gaattggaag ccgaagaaga tgggtacttg attgccattc tcaaaggaga tggtgaaact
     7801 gtccctgtaa cggaagttat cggttacctt ggtgaagaaa gggaaaacat cccaacagct
     7861 ggagcagcat cgccagaagc tagccctgta cctgtagcaa gtacctcaaa cgatgatggt
     7921 aagagcgatg atgcttttga tatcgttgtg attggtggag gtcctgctgg ttatgttgca
     7981 gccattaaag ctgcccaact cggcggtaag gttgcccttg ttgagaaatc tgaacttggt
     8041 ggaacctgct taaaccgtgg atgtattcca accaagacct accttcatag cgctgaaatt
     8101 attgaaaata tcggtcatgc tgcaaatcgt ggtatcgtga ttgaaaatcc taacttcact
     8161 gttgatatgg aaaaactttt agaaactaaa tctaaagttg ttaatactct tgttggtggt
     8221 gttgcaggac ttcttcgtag ctacggagtt gctgttcata aaggaattgg tacaatcact
     8281 aaagacaaga acgtcttggt aaatggttct gaattgcttg aaaccaagaa aatcattctt
     8341 gctggtggtt caaaagtcag caagatcaac gtccctggta tggaatctcc acttgtcatg
     8401 actagtgatg acattcttga aatgaacgaa gtgccagaaa gtcttgttat catcggtggt
     8461 ggagttgtcg gtatcgaact cggtcaggcc ttcatgacat ttggttcaaa agtgactgtt
     8521 atcgaaatga tggaccgtat agttccagct atggatgtgg aagtttctaa gaaccttcgc
     8581 ttgatccttg agcgtaaagg aatgaccatc ttgactggta ctaaactgca agaaatcatt
     8641 gaggaaaatg gtcaacttcg tatcaaggtt gaaggaaaag acgatatcat cgcaagcaaa
     8701 gctcttcttt caattggtcg tatgccagac cttgaaggta ttggagaggt tgagtttgaa
     8761 ttggatcgtg gttgtatcaa ggtcaacgaa tacatggaaa cttcagttcc aggcatttat
     8821 gcaccaggtg acatcaacgg tactaagatg ttggctcacg cagctttccg catgggtgaa
     8881 gtttccgctg aaaatgccct taaaggaaat catgcagttg ccaaattgaa tttgactcct
     8941 gcagccatct acactcttcc tgaagtagca gcagtaggtt tgacagaaga acaagcccgt
     9001 gagaaatacg atgttgccat cggtaagttt aactttgctg ctaacggtcg tgctattgca
     9061 tctgacgcag ctcaaggttt cgtaaaagtt atcgctgata agaaatacgg agaaatcctt
     9121 ggtgtacaca ttattggtcc tgcagccgca gaattaataa acgaagcatc aagcatcatc
     9181 gaaatggaaa tcactgttga ggaaatgctg aagaccatcc acgggcaccc aacttactct
     9241 gaagtgatgt acgaagcgtt tgcagatgtt ctaggaatgg ccatccattc acctaagaaa
     9301 aaataaaacg aagatagatt agactggaag gttatttcca gtttctatca tataaagtaa
     9361 agggatatca tgaaatacat tatcaatcat tcaaatgaca ctgcttttaa tattgccttg
     9421 gaagaatacg cctttaaaca ccttttggat gaggatcaaa tcttcctact ttggattaac
     9481 aagccatcta tcattgttgg tcgtcaccag aacactatcg aagaaatcaa ccgtgattat
     9541 gttcgcgaaa ctggtattga ggtagttcgc cgtattagtg gtggtggagc tgtttaccac
     9601 gatttaaata acctcaacta cacgatcatc tccaaagaag atgaaaataa ggcatttgac
     9661 ttcaagagct tctcaactcc ggttatcaat accctggctc aactcggggt taaagctgag
     9721 ttcacaggtc gtaatgacct tgagattgat ggtaagaagt tctgtggcaa tgcccaagcc
     9781 tatatcaacg gccgtatcat gcaccacggt tgcttgctct ttgacgttga tttgtcagtc
     9841 ctcgcaaatg cccttaaggt ttcaaaagat aaatttgaat caaaaggtgt gaaatccgtc
     9901 cgtgcccgtg taaccaatat tatcaatgaa ttaccaaaaa aaatcacagt cgaaaaattc
     9961 cgtgatttac tcttggaata catgaaaaaa gagtacccag agatgactga atacgtcttt
    10021 tcagaagaag aattggccga aataaatcgc atcaaggata ctaagtttgg aacttgggac
    10081 tggaactatg gtaaatcacc tgaatttaac gtccgtcgtg gaaccaaatt cactagtggt
    10141 aaggttgaag tctttgctaa cgttactgaa tcaaaaatcc aagacatcaa gatttatggt
    10201 gacttctttg gtattgaaga cgttgctgca gtagaagctg tccttcgtgg ggtgaaatac
    10261 gaacgtgaag atgttcttaa ggcgctaaaa accattgata tcacacgcta cttcgctggt
    10321 attagccgtg aagaaatcgc tgaagcggta gttggataaa tcaaaaagaa gttggccaac
    10381 taaccaactt ctttttttga tatttattta cataatttaa aatacgtaaa atcataaact
    10441 atccagagca ttcttttgtt catcactaac aatatgggta tagaggtcag tgacttgtgt
    10501 gctagcatgt cctagttggt gactgactaa aacttgtgat ttagtcgcat catagagcct
    10561 agttgctagt gtatggcgca gtttatgggg tgttacacgc actttaaaat cctctgagta
    10621 tttagcaacc attttctcaa cgctagaagc atcgatacga ttaggaacac ctctgtagag
    10681 agttaaaaaa agggctgtat ctgttttttc cgttttatag cgttgattcc gaatggccag
    10741 ataattctct aaataaggtt tagcaaaagc agcgacattg actgagtcac gtttgccacc
    10801 ttttcgagta acatcaataa ccatcatttt tagattgaga tctcttagat ctagattaac
    10861 agcttcagat aagcgaacac cagatgccaa gagaagggca ataatggcta aatctcgttc
    10921 tttatttttg ttgaatgatg agagagctcg atttgaaagc tgttgtgggt gctcttgatc
    10981 gatataagtt agaaaacctt ctgtttcatc acctagaaag agtttttgct tgatattttc
    11041 agctctggca gcaagggttt ctttcttttt cttggttgaa acttttttca ttacattacg
    11101 atagaaataa ggttccccct gatcgttttc aacctcctcg gttagatact tgtaaagact
    11161 agaaagtgct gataaggttc gattgatagt tgtctgtgaa acaccttgtt ttgttgtatt
    11221 agcattcagc aagggacgtt cacgtagata aaggataaag gattccatgt ctttcttaga
    11281 catattttcc aaaactgata aaggaatatc ggatatttta tcagcgtttg aaataccaga
    11341 ctccaaaacc cagctgaaaa atcggtcata ttccttaagg tattcgtaca gggttgtaaa
    11401 actgtagggc acagccagtt tagattggta gtattccaga acataccagg gcatgagttg
    11461 ttttagtttg tcgattcgtt ccagtaaaat ctcacgtttc atgtatttcc tccataatta
    11521 agtat
//
LOCUS       DAVKJD010000056        11337 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKJD010000056 DAVKJD010000000
VERSION     DAVKJD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11337
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.056"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(128..1739)
                     /locus_tag="VW157_001515"
                     /pseudo
     CDS             complement(128..1739)
                     /locus_tag="VW157_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
     gene            2028..6878
                     /locus_tag="VW157_001516"
     CDS             2028..6878
                     /locus_tag="VW157_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU9552794.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHTRENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQKP
                     KKNYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGH
                     EVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHEISTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLDAKPNHPSGS
                     ALAPLLEKNKVLVEKVDLSPEELATAKQSLKDLVALLKVERVQASAEEKKYFAGEDAH
                     VFEIEGLDEKGQDVDLSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIF
                     TAPHFTHYAFVYESAEKPQPAKPAPQNKVLPKPTYQPASDQQKAPKLEVQEEKVAFHR
                     QEHENAEMLVGEQRVIIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKV
                     KTVPAVVATQEKPAQNTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRR
                     KREDKD"
     gene            6968..8164
                     /locus_tag="VW157_001517"
     CDS             6968..8164
                     /locus_tag="VW157_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9552795.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8300..8827
                     /locus_tag="VW157_001518"
     CDS             8300..8827
                     /locus_tag="VW157_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9552796.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            8904..9260
                     /gene="glnR"
                     /locus_tag="VW157_001519"
     CDS             8904..9260
                     /gene="glnR"
                     /locus_tag="VW157_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9552797.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9297..10643
                     /gene="glnA"
                     /locus_tag="VW157_001520"
     CDS             9297..10643
                     /gene="glnA"
                     /locus_tag="VW157_001520"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9552798.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10733..10852)
                     /locus_tag="VW157_001521"
     CDS             complement(10733..10852)
                     /locus_tag="VW157_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9552799.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
BASE COUNT         3623 a         2086 c         2456 g         3172 t
ORIGIN      
        1 tagaggtgta ctattctagt ttcaatatac tatatatctg cagaatactc tttcagtttc
       61 attcagccta gtcttgctta ggaaaaagta aaaagtcatc caattatgat ggatagcttc
      121 ttggttctta gatttgttct gcaaaaacct tttcagcaag gttcatagcg tggtctgata
      181 cacgagtgta gtgtgagatg atgtcgataa agttgacccc agcttgtgtt gaacattcgc
      241 ctttgttgag gcgtttgatg tgggtttttc tgagaacacg ttctatctta ttgattgctt
      301 catgacgttc tacaagactg cgtgcttttt caatatcatt gttttccaca ctatccagag
      361 catctttgat aaagtcacta gtttggcggt aaacttcctc taattctttc aaggcggcat
      421 cagaaaattc aacatttttc cgttgaagat agtcagtcag attgagtaga gcctccgtgt
      481 ggtctccaat ccgttccaaa tcacgggagg aatcaaggat attggtaagc acttcacttt
      541 ctttttggct gagagcttcg cttgaaaggg caatgagata acgtgttaat tgcttcttcg
      601 gttttatgcc ctttttcagc aactttttca tccaagtcaa tgatgtactt ataggaaagg
      661 tcaaaggctt tagcagcgta gtttcctaag tgcaagagct ctttcttagc atttcctaga
      721 gcgatagatg gggcctgttt gatgaaatgt tcatcaagat ataagggttc gtatttgaca
      781 acctcgtcct ctccaggaat aatcttggtt acaaagtaag ccagagctcc gataaatgga
      841 aattggacaa tggtgttggt aatattaaag gttccgtgag caaaggcgat ggtcatttcc
      901 ggtgctagat ttagcgtagc ttcaaaccaa tgaatcagga cagtaaaagg aactagaaaa
      961 ataacgcaga caactgttcc gataacgttg aaggcaacat gagctcctgc tacccgttta
     1021 gctgcaatat tagcccctaa agaggcaatg atggctgtaa tggttgtccc gatattgtca
     1081 ccaaatagaa ccggcaaagc tccctgtagg tcaattagat tgccggcgta gaggttttgt
     1141 aaaatcccaa tggtagccga agaagcttga attagcaagg tcaagccagt accgacaaag
     1201 acacccaaaa caggattttt acttagctca atcatatagt ccttaaagac ctgtaaatcc
     1261 ttgagtggag ccattgcgcc gctcatgaga ttgagggcaa aaaagatacc accgacacca
     1321 aagaggatgc gtccgatatt attgactgtc cgattttttg taaaaaagag acagacggca
     1381 ccgataaaga gcataggtag ggcatagtta cctaatttaa aaccgatgag aaaggatgtg
     1441 acagttgtcc caatattagc acccatgaca atcccgatag cctgacgtaa ggttaagaga
     1501 ccggcactga ccaggccgac tgtgataact gttacaccag aactagactg aattagagca
     1561 gtcatcccaa taccaaccag aactccaaag aaaggattac tagtatattt gtcaatgtaa
     1621 aaacgaaggc gatctccagc agcttgttgt aaaccgtctc ccatggtctt gatactatat
     1681 aagaatagcc ccagaccacc taaaaagtga aataaaattt cctgccaatt aatggacatt
     1741 tctttttcct ccgaaaaata atcgcggaat atctcctatt ctattttaaa ggataaaagt
     1801 aaatctaaca agtgttaatg tcaagtttgt aaaagaaaat tataattgat aagtgtatta
     1861 tttttaagag tttttaacct tattttagat ttgtgaaaac agctattgtt ttttgtctaa
     1921 aaatgtttta ggataaaaat gtgattatca tgattctaga gttaaaaaac tacagaatat
     1981 aaagcgcttt cttgaaaaca acaaaatcaa tcttttagga ggagaaaatg aagaatccat
     2041 tttttgaaag acgttgtcgt tacagtattc gtaagttatc agtaggagcc tgctcgctga
     2101 tgattggtgc tgttttattt gctggtccag ccttggctga agaaactgca gttcctgaaa
     2161 atagcggagc taatacagag cttgtttcag gagagagtga gcattcgacc aatgaagctg
     2221 ataagcagaa tgaaggggaa catactagag aaaacaagct agaaaaggca gaaggagtag
     2281 cgacagcatc tgaaactgca gaagcagcta gcgcagctaa accagaggaa aaagcaagtg
     2341 aggtggttgc agaaacacca tctgcagaag caaaacctaa gtctgacaag gaaacagaag
     2401 caaagcccga agcaactaac caaggggatg agtctaagcc agcagccgaa gctaataaga
     2461 ctgaaaaaga agtccagcca gatgtcccta aaaatacaga aaaaacatta aaaccaaagg
     2521 aaatcaaatt taattcttgg gaagaattgt taaaatggga accaggtgct cgtgaagatg
     2581 atgctattaa ccgcggatct gttgtcctcg cttcacgtcg gacaggtcat ttagtcaatg
     2641 aaaaagctag caaggaagca aaagttcaag ccttatcaaa caccaattct aaagcaaaag
     2701 accatgcttc tgttggtgga gaagagttca aggcctatgc ttttgactat tggcaatatc
     2761 tagattcaat ggtcttctgg gaaggtctcg taccaactcc tgacgttatt gatgcaggtc
     2821 accgtaacgg ggttcctgta tacggtacac tcttcttcaa ctggtctaat agtattgcag
     2881 atcaagaaag atttgctgaa gctttgaagc aagacgcaga tggtagcttc ccaattgccc
     2941 gtaaattggt agacatggca aagtattatg gctatgatgg ctatttcatc aaccaagaaa
     3001 caactggaga tttggttaaa cctcttggag aaaagatgcg ccagtttatg ctctatagca
     3061 aggaatatgc tgctaaggta aaccatccaa tcaagtattc ttggtatgat gccatgacct
     3121 ataactatgg acgttaccat caagatggtt tgggagaata caactaccaa ttcatgcaac
     3181 cagaaggaga taaggttccg gcagataact tctttgctaa ctttaactgg gataaggcta
     3241 aaaatgatta cactattgca actgccaact ggattggtcg taatccttat gatgtatttg
     3301 caggtttgga attgcaacag ggtggttcct acaagacaaa ggttaagtgg aatgacattt
     3361 tagacgaaaa tgggaaattg cgcctttctc ttggtttatt tgccccagat accattacaa
     3421 gtttaggaaa aactggtgaa gattatcata aaaatgaaga tatcttcttt acaggttatc
     3481 aaggagaccc cactggccaa aaaccaggtg acaaagattg gtatggtatt gctaacctag
     3541 ttgcggaccg tacgccagcg gtaggtaata cttttactac ttcttttaat acaggtcatg
     3601 gtaaaaaatg gttcgtagat ggtaaggttt ctaaggattc tgagtggaat tatcgttcag
     3661 tatcaggtgt tcttccaaca tggcgctggt ggcagacttc aacaggggaa aaacttcgtg
     3721 cagaatatga ttttacagat gcctataatg gcggaaattc ccttaaattc tctggtgatg
     3781 tagctggtaa gacagatcag gatgtgagac tttattctac taagttagaa gtaactgaga
     3841 agaccaaact tcgtgttgcc cacaagggag gaaaaggttc taaagtttat atggcattct
     3901 ctacaactcc agactacaaa ttcgatgatg cagatgcatg gaaagagcta accctttctg
     3961 acaactggac aaatgaagaa tttgatctca gctcactagc gggtaaaacc atctatgcag
     4021 tcaaactatt tttcgagcat gaaggtgctg taaaagatta tcagttcaac ctcggacaat
     4081 taactatctc ggacaatcac caagagccac aatcgccgac aagcttttct gtagtgaaac
     4141 aatctcttaa aaatgcccaa gaagcggaag cagttgtgca atttaaaggc aacaaggatg
     4201 cagatttcta tgaagtttat gaaaaagatg gagacagctg gaaattacta actggctcat
     4261 cttctacaac tatttatcta ccaaaagtta gccgctcagc aagtgctcag ggtacaactc
     4321 aagaactgaa ggttgtagca gtcggtaaaa atggagttcg ttcagaagct gcaaccacaa
     4381 cctttgattg gggtatgact gtaaaagata ccagcctacc aaaaccacta gctgaaaata
     4441 tcgttccagg tgcaacagtt attgatagta ctttccctaa gactgaaggt ggagaaggta
     4501 ttgaaggtat gttgaacggt accattacta gcttgtcaga taaatggtct tcagctcagt
     4561 tgagtggtag tgtggatatt cgtttgacca agccacgtac cgttgttaga tgggtcatgg
     4621 atcatgcagg ggctggtggt gagtctgtta acgatggctt gatgaacacc aaagactttg
     4681 acctttatta taaagatgca gatggtgagt ggaagctagc taaggaagtc cgtggtaaca
     4741 aagcacacgt gacagatatc actcttgata aaccaatcac tgctcaagac tggcgcttga
     4801 atgttgtcac ttctgacaat ggaactccat ggaaggctat tcgtatctat aactggaaaa
     4861 tgtatgaaaa gcttgatact gagagtgtca atattccgat ggccaaggct gcagcccgtt
     4921 ctctaggcaa taacaaggta caagttggct ttgcagatgt accggctgga gcaactatta
     4981 ccgtttatga taatccaaat tctcaaactc cgcttgcaac cttgaagagc gaagttggag
     5041 gagacctagc aagtgcacca ttggatttga caaatcaatc tggtcttctt tattatcgta
     5101 cccagttgcc aggcaaggaa attagtaatg tcctagcagt ttccgttcca aaagatgaca
     5161 gaagaatcaa gtcagtcagc ctagaaacag gacctaagaa aacaagctac gccgaagggg
     5221 aggatttgga ccttagaggt ggtgttcttc gagttcagta tgaaggagga actgaggacg
     5281 aactcattcg cctaactcac gcaggtgtat cagtatcagg ttttgatacg catcataagg
     5341 gagaacagaa tcttactctc caatatttgg gacaaccagt aaatgctaat ttgtcagtga
     5401 ctgtcactgg ccaagacgaa gcaagtccga aaactatttt gggaattgaa gtaagtcaga
     5461 aaccgaaaaa aaattaccta gttggtgata gcttagactt gtctgaagga cgctttgcag
     5521 tggcttatag caatgacacc atggaagaac attcctttac tgatgaggga gttgaaattt
     5581 ctggttacga tgctcaaaag actggtcgtc aaaccttgac gcttcgttac caaggtcatg
     5641 aagtcaactt tgatgttttg gtatctccaa aagcagcatt gaacgatgag tacctcaaac
     5701 aaaaattagc agaagttgaa gctgctaaga acaaggtggt ctataacttt gcttcaccag
     5761 aagtaaaaga agctttcttg aaagcaattg aagcggccga acaagtgttg aaagaccatg
     5821 aaattagcac ccaagatcaa gtcaatgacc gacttaataa attgacagaa gctcataaag
     5881 ctctgaatgg tcaagagaaa tttaaggaag aaaagacaga gcttgatcgt ttaacaggtg
     5941 aggttcaaga actcttggat gccaaaccaa accatccttc aggttctgcc ctagctccgc
     6001 ttcttgagaa aaacaaggtc ttggttgaaa aagtagattt gagtccagaa gagcttgcaa
     6061 cagcaaaaca gagtctaaaa gatctggttg ctttattgaa agtagagcgt gttcaagcaa
     6121 gtgctgaaga gaagaaatac tttgctggag aagatgctca tgtctttgaa atagaaggtt
     6181 tggatgaaaa aggtcaagat gttgatctct cttacgcttc tattgtgaaa atcccaattg
     6241 aaaaagataa gaaagttaag aaagtatttt tcttacctga aggcaaagag gcagtagaat
     6301 tggcttttga acaaacggat agtcatgtta tctttacagc accacacttt actcattatg
     6361 cctttgttta tgaatctgct gaaaaaccac aacctgctaa accagcacca caaaacaaag
     6421 tccttccaaa acctacttat caaccggctt ctgatcaaca aaaggctcct aaattggaag
     6481 ttcaagagga aaaggttgcc tttcatcgtc aagagcatga aaatgctgag atgctagttg
     6541 gggaacaacg agtcatcata cagggacgag atggactgtt aagacatgtc tttgaagttg
     6601 atgaaaacgg tcagcgtcgt cttcgttcaa cagaagtcat ccaagaagcg attccagaaa
     6661 ttgttgaaat tggaacaaaa gtaaaaacag taccagcagt agtagctaca caggaaaaac
     6721 cagctcaaaa tacagcagtt aaatcagaag aagcaagcaa acaattgcca aatacaggaa
     6781 cagctgatgc taatgaagcc ctaatagcag gcttagccag ccttggtctt gctagtttag
     6841 ccttgacctt gagacggaaa agagaagata aagattaaat atcgaaaaat cttgtgaaat
     6901 ctttccttat atttccaaag tgtgatataa tagtttcgaa taaaataaat aaaggagttt
     6961 ttgtaacatg gcaaaactta ctgttaaaga cgttgacttg aaaggtaaaa aagtcctcgt
     7021 tcgtgttgac ttcaacgtac cattgaaaga tggcgtaatc actaacgata accgtatcac
     7081 agcagctctt ccaactatta agtacatcat cgaacaaggt ggacgtgcaa ttcttttctc
     7141 tcaccttgga cgtgtgaaag aagaagctga taaagctggt aaatcacttg ctcctgtagc
     7201 agctgacttg gcagcaaaac ttggtcaaga tgttgttttc ccaggtgtca ctcgtggtgc
     7261 tgaattggaa gcggcaatca acgctcttga agatggacaa gttctcttgg ttgaaaacac
     7321 tcgttacgaa gatgttgacg gcaagaaaga atctaaaaac gatcctgaac ttggtaaata
     7381 ctgggcatca cttggagatg gtatcttcgt aaacgatgca ttcggtacag ctcaccgtgc
     7441 acacgcatct aacgttggta tctcagcaaa cgttgaaaaa gcagttgctg gtttccttct
     7501 tgaaaacgaa attgcctaca tccaagaagc agttgaaact ccagaacgtc cattcgtggc
     7561 tatccttggt ggttcaaaag tttcagacaa gatcggtgtt atcgaaaact tgcttgaaaa
     7621 agctgataaa gtccttatcg gtggtgggat gacttacaca ttctacaaag cacaaggtat
     7681 cgaaatcggt aactcacttg tagaagaaga caaattggat gttgcgaaag ctcttcttga
     7741 aaaagcaaat ggtaaattga tcttgccagt tgactcaaaa gaagctaacg catttgctgg
     7801 ttacactgaa gtgcgtgaca ctgaaggtga agcagtttct gaaggcttcc ttggtcttga
     7861 catcggtcca aaatctatcg ccaaatttga cgaagctttg actggtgcca aaacagttgt
     7921 atggaacgga cctatgggtg tatttgaaaa cccagatttc caagctggta caatcggtgt
     7981 gatggacgct atcgtgaaac aaccaggagt taaatcaatc atcggtggtg gtgactcagc
     8041 tgccgcagcg attaaccttg gccgtgcaga caagttctca tggattagta cgggtggtgg
     8101 agcatcaatg gaacttcttg aaggtaaggt tcttccagga cttgcagcct tgacagaaaa
     8161 ataagatttt ataaataaat caaagaagag agggatgaaa gttcctcttt tcttttgctt
     8221 aaaataaaaa cgcttcctct caactattac tcataaaaat caccgattta tgataaaatg
     8281 gaaatagaaa gttgagatta tgagttattt taaaaaatat aaattcgata aatcccagtt
     8341 caaacttggt atgagaacct ttaaaacagg tattgctgtt tttctagttc tcttgatttt
     8401 tggctttttt ggctggaaag gtcttcaaat tggtgctttg acagccgttt ttagcctgag
     8461 ggagagtttt gatgagagtg ttcattttgg gacttcgcgt attctaggaa atagtatcgg
     8521 tggactctat gccttggtct tcttcttatt aaataccttt ttccatgaag ccttttgggt
     8581 gaccttggta gttgttccaa tctgcaccat gttaaccatt atgacaaatg tagccatgaa
     8641 taacaaagca ggggttattg gtggtgtagc agctatgtta atcattaccc tatcaattcc
     8701 aagtggtgag acaatttcgt acgtgtttgt gcgtgtatca gaaacgttta tgggagtttt
     8761 tgtcgcaatt atcgtaaatt acgatattga tcgtattcgt ctctttttag agaaaaaaga
     8821 aaaataatgt tacattttat aacattatca attgacgttt gtcttttttt agactataat
     8881 agacagaaag aaggaaattg taaatgaagg aaaaagaatt tcgccgaaat atggctgttt
     8941 ttcctatcgg cagtgttatg aagttgaccg atctatcggc gcgtcagatt cgttattatg
     9001 aagatcaaga gttgatcaag cccgatcgaa acgaaggaaa tcgtcgcatg tattccttga
     9061 atgacatgga tcgtctgctt gaaatcaaag attatatctc tgaaggttat aatatcgctg
     9121 ccattaagaa aaaatatgct gaacgtgaag cgaaatccaa gaaagcggtt agtcagactg
     9181 aagtacgtcg tgcacttcac aatgaactcc tccaacaggg gcgttttgct tcagtacagt
     9241 caccttttgg tcgcggttag gcaaccgcaa gtagtcatag attaaggaga aaacttatgc
     9301 caatcacagc tgcagatatt cgtcgtgaag tcaaggaaaa aaatgttacc tttattcgtc
     9361 ttatgttctc agatattttg ggaaccatga aaaacgtcga aattcctgct acagatgaac
     9421 agttagataa ggtcttgtcg aacaaggtta tgtttgatgg atcttctatt gaaggttttg
     9481 tacgtatcaa tgagtcggat atgtacttgt acccggactt ggatacatgg acagtcttcc
     9541 cttggggaga tgaaaatgga agtgttgcag gtctgatctg tgatgtctat acaacagaag
     9601 gtgaaccatt tgcgggtgac cctcgtggta atttgaaacg agctcttcgt cacatggaag
     9661 aagttggatt caaatccttc aaccttggtc cagagccaga attcttccta tttaagttgg
     9721 atgaaaatgg ggacccaaca cttgaagtga atgacaaggg tggctacttt gatttggcac
     9781 ctactgacct tgcggacaac acacgtcgtg agattgtgaa tgtcttgacc aaaatgggat
     9841 ttgaagtaga agcgagtcac cacgaggttg cggttggaca gcatgagatt gactttaagt
     9901 acgatgaagt tctccgtgct tgtgataaga ttcaaatctt taaacttgtt gttaaaacca
     9961 ttgctcgcaa acacggactt tacgcaacat ttatggcgaa gccaaaattt ggtattgctg
    10021 gctcaggtat gcactgtaat atgtccttgt ttgatgcaga aggaaataac gccttctttg
    10081 atccaaatga tccaaaagga atgcagttgt cagaaacagc ttaccatttc ctaggcggtt
    10141 tgatcaagca tgcttacaac tatactgcca tcatgaaccc aacagttaac tcatacaaac
    10201 gtttggttcc aggttatgaa gcgcctgttt acattgcttg ggctggtcgt aaccgttcgc
    10261 cacttgtgcg cgtacctgct tcacgtggta tgggaactcg tcttgagttg cgttcagtgg
    10321 atccaatggc gaacccttac attgctatgg ctgttctttt ggaagttggt ttgtatggta
    10381 ttgaaaataa aatcgaagca ccagctccta tcgaagaaaa tatctacatc atgacagcag
    10441 aagagcgcaa ggaagctggt attacagacc ttccatcaac tcttcataat gctttgaaag
    10501 ctttgacaga agatgaagtg gttaaagctg ctctcggaga tcacatctac actagcttcc
    10561 ttgaagccaa acgaatcgaa tgggcaagtt atgcaacctt cgtttcacaa tgggaaattg
    10621 ataattattt agacctttac taatatagaa gaaagattgc ctgtgggtaa acagtcacac
    10681 agcagtctaa gccatcaaat acctataacc cttaaaatag cgatttcggt cgctatttat
    10741 ttttcgaaaa atcctcttga ctcttttgcg aattagattt atcatcagac gtgtagaaga
    10801 aaatcaagga gtcactatgg aaaaattcaa tttcaaaaac aatatcgggc aagaaaacaa
    10861 gttacttcaa atcgaaatct acaagtttac taacttttgc aagcttcaaa attacacatc
    10921 tgtaaatatt ttttcgaaag atatatttga agccatagta aattgagact agaatagtac
    10981 actacatctg ctaaaaattt ctagaaatta atttaacttt cctaatcgat ttgtttacat
    11041 cttatttcaa tctactatat tatctcaata aatgatgatg gcgactgttt tgtttgtgct
    11101 tgtcgatttt tgaatcctga tcgagcggaa gaatatttct gccgaggttg tcaaggggaa
    11161 aatcctgaag atattgaatt ttatgatgaa caattacagg cagaaaaggt ggaggctttg
    11221 aatattcgat tggaagtaaa aaattgaatt atctacaatc tcatcaacaa taaacctatt
    11281 tcaaccccat tatctatagg cgtattttta cgccttttta tatacttgaa gcacata
//
LOCUS       DAVKJD010000057        11171 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKJD010000057 DAVKJD010000000
VERSION     DAVKJD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11171
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.057"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            525..896
                     /locus_tag="VW157_001522"
     CDS             525..896
                     /locus_tag="VW157_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552800.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            1525..1908
                     /locus_tag="VW157_001523"
     CDS             1525..1908
                     /locus_tag="VW157_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9552801.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            1960..2649
                     /locus_tag="VW157_001524"
     CDS             1960..2649
                     /locus_tag="VW157_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9552802.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            2691..2924
                     /locus_tag="VW157_001525"
     CDS             2691..2924
                     /locus_tag="VW157_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552803.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            3075..3686
                     /locus_tag="VW157_001526"
     CDS             3075..3686
                     /locus_tag="VW157_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9552804.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            3847..4641
                     /locus_tag="VW157_001527"
     CDS             3847..4641
                     /locus_tag="VW157_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9552805.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            4638..5273
                     /gene="trmB"
                     /locus_tag="VW157_001528"
     CDS             4638..5273
                     /gene="trmB"
                     /locus_tag="VW157_001528"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9552806.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            5399..5878
                     /gene="rimP"
                     /locus_tag="VW157_001529"
     CDS             5399..5878
                     /gene="rimP"
                     /locus_tag="VW157_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9552807.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            5922..7058
                     /gene="nusA"
                     /locus_tag="VW157_001530"
     CDS             5922..7058
                     /gene="nusA"
                     /locus_tag="VW157_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9552808.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7080..7373
                     /locus_tag="VW157_001531"
     CDS             7080..7373
                     /locus_tag="VW157_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9552809.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            7366..7665
                     /locus_tag="VW157_001532"
     CDS             7366..7665
                     /locus_tag="VW157_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9552810.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLAKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            7682..10474
                     /gene="infB"
                     /locus_tag="VW157_001533"
     CDS             7682..10474
                     /gene="infB"
                     /locus_tag="VW157_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9552811.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKTEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            10725..11075
                     /gene="rbfA"
                     /locus_tag="VW157_001534"
     CDS             10725..11075
                     /gene="rbfA"
                     /locus_tag="VW157_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9552812.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3423 a         1992 c         2486 g         3270 t
ORIGIN      
        1 ttaattagaa aaatattttc aaaatcttcc aaaaaggata cggatagtac tctctaaaaa
       61 ataaatttct cccctgtatt tgctagctta atttacaggg gagtttcttt aatattgttt
      121 cgcctgtcaa aatgatatgt gatagcagga tgaaaagtct acaaacaagt tggtatcaat
      181 acaatataaa gaggaaagta tgtttttcat gcttgctttt ttaattttta caattcaaga
      241 ggttttgatg accatttatg atttgagtga tccaaggtca aaataagtgc tatactagca
      301 gtgtaaatgt tcttgctcaa ctaagataaa tcgcacattt aggaggaaaa cttatgaaga
      361 aaaaaatact gattattttc gttctgtatc tgatcatgtc catctttctt tatccgctta
      421 gggagagtat ttggtataat ctattttata ccatagccta tatgattgcg gttatgatct
      481 atttttcttt aattaaaaag aaagaaaaga aatgaaagat tttcttgatt aaaaagaatc
      541 atattctttg tgagattgtt cctgggtcag ttgcctttgc ttgtttctac ttatctattt
      601 ctatttcgtc agttttccaa tttcttttag tagttattaa cttgacttct attttggtta
      661 ctgtttatct tactagggaa atgagattaa gagagtttga agatgatgat ttggttagtc
      721 ctagaaccaa tcaactcatg tttatcggct tgacaggctt tatgtctatt atttgtttgt
      781 atagaggtat cacagcagga gaatcttatc aacaactaat cgcttatatt ggtgctattc
      841 tttgcttgat catcatgctt ctactcattt ggggtttgaa gtattataaa aagtaggggc
      901 taatgaagtt tttgcaattt aggaggtttc gatgacaatt caagatttgg atgaaaaatt
      961 ttaaaaaaca atgatatact aaacttgtca aagttgcaac gggtgctgct acattatgtt
     1021 aaaaatcgct cttgattgat aagttggatt tgttaggaaa ggagtaaata attatggata
     1081 agaaaaagat agtttcgact ataatatgta tagtatttct tgtagtgtca gtagataatt
     1141 tttttagaga cttaaccccg ttactgttca ttttaaatat tattggcctg tcttgctttt
     1201 cagtgctaac atacattaac ataaaagaga tactcttaaa tataagtaaa tgatgatttg
     1261 gttagtccta gaaccaatca acttatgttt atcggcttgg cagattttat gtttgtcatt
     1321 tgtttgtata gaggtatttc agaaacagaa ttctatcaac aactaatcgc ttatataggt
     1381 gtattctctg cttgtttatc acgcttctgc tcatgtgggg cttgaagtat tataaaaagt
     1441 agctttctat ccttttctga caggatattt gagtagaagg ctctattaaa ttgttagtga
     1501 aataaatgaa tggaggtatt taatatgaaa tacagattat tttttgttat tttcttgagt
     1561 agtgtgttgg atattctttt agggacattt ttacaaatct ctatcgtatc tattgggtgg
     1621 cttgttcttt acagtggttt gtttgaagca ggagttttcc ttcttgctaa taaaggggtg
     1681 gcggtaaaaa tcaaggaagt agatattcga aatcgcttta aatttatttt tggaaaaacc
     1741 ttatggtttc aaattctttt gctcatcttt ttgataatca aactttatct tggtttggat
     1801 gcgaggttga ttttattcta tggacatatt ttcattgtct ttaatgcctt aatgtatcta
     1861 ttatctagta gtcaggttag ccttaaaaaa aacaaactgt cttcttaatc ttacagcagt
     1921 agagcctgac aaggtgaggt catagtaggg aggatttcta tgaaaaagta tcaacttcta
     1981 ttcaaaataa gtgcagtttt ctcttactta tttttcgtat ttggtctttc tcagatgacg
     2041 cttattattc aaaattattg gcaattttct tcccagattg gcaatttcgt ctggattcaa
     2101 aatttcttga gtttgctatt tagcggagtc atgatttgga ttctggttaa gacaggtcat
     2161 ggttatctct ttcacattcc aagaaaaaaa tggctttggt attcgatttt gacagtatta
     2221 gtggtagtgc tccagatctc ttttaacgtt cagacagcta aacatgttca gtcaactgct
     2281 gaaggttggg ctgtattgat cggttatagt gggaccaact ttgctgagct aggtatctat
     2341 ataactttgt tctttctgac tccacttatg gaagagctaa tctatagagg attactgcaa
     2401 cacgcctttt ttaagcattc gagatttggc cttgatttgc ttcttccgtc aattttattt
     2461 gctcttcctc atttttcaag cctgcctagt ttgttagata tcttcatctt tgcaacatct
     2521 ggcatcatct ttgctagttt gacccgctat accaagagca tttatccttc ctatgcggtg
     2581 catgtgatca ataatatttt cgcaacatta ccatttttgc tgactttttt acatagggtc
     2641 ttggggtaaa aaaacaaaag gcttgctttt cagccataga ggagattatc atgtataaac
     2701 acttattttt cctagattcc aaaactttag atcggttgac accctatatt ctagtcttgg
     2761 cttctgacac cattgctttt aatgtttttg tgctaacctt tgtatctgcg gtggttttta
     2821 atttcctaaa ttccatgcta gctttaatgg ctatattcat aggggctggc tatgtggtcg
     2881 gattttggtt actaatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc
     2941 tgtacttgag tacggcaagg cgacgttgac gtggtttgaa tttgattttc gaagagtata
     3001 aaatggtttg ttttggaaag gctagaacta aagaatgacg tgtaggaagt ctgtttgttg
     3061 aggaggataa ttttatggag ttttttgata aatttcatgc cttgtgtttt ggatttttag
     3121 tactaataat tgtcattaca gttccttata cgattaacca tggggatttt tttcaaaatg
     3181 aatctgcatt gattattgta agtcttcttg taacctcgct gagtgttgct tatgctagaa
     3241 agtttgaaat gatttctttt gggatgttaa gcaagaaaca acttttgctt ttcattgcaa
     3301 tctttcttct aagtgtactt gagacgctgg tttatattca tttcttcgct gtctcttctg
     3361 gctcaggggt ccaacacttg gcggaagtca gcagaggaat ttccctgtct ttgattttga
     3421 ctacctcagt ttttggcccc atccaggagg aactcatttt cagaggactt cttcaaggtg
     3481 cggtttttga caattcttgg ttagggcttg tgctaacttc ctctctcttt tctttcatgc
     3541 atggaccttc taatgtccct tcgtttattt tttatctact tgggggcttg ttgctgggcc
     3601 ttgcttataa aaagagccaa aacctatggg tttctactct agtccacatg ttttacaaca
     3661 gttggccact cctatattat ttataaaaat tatgaaaagg taagcagaag acggcgctta
     3721 cctttttcaa gctataatga tacccaagcc aatccagagg cttttctttg agaatcgggt
     3781 tagagtggtt gacgaatagg ccaaaaacta gtagaatagt aaggaaactt tatacggagg
     3841 aaagaaatgg atttgggtga taatgagcta acactgactc ccatacctgg gaaaagtggc
     3901 aaggcttata tgggtagcta tcctgatggg aagcgcatct ttgtaaaaat gaacacctct
     3961 ccaatcctac ctggtctagc tagagaacaa attgctccac aattattatg gagtcgtcgt
     4021 ttggcagatg ggcgtgatat gtgtgctcaa gaatggttga caggcaagat attgaccccc
     4081 tatgatatga atcgtaagca aatcgtcaat attttaaccc gtcttcatcg ctcacgtccg
     4141 ttgatgacac aattgagtcg tttgggctat gccatggaaa cacctgtaga tttactacag
     4201 tcttggcaag aaacggctcc agatgctttg cgtaaaaatc attttatcag tgaagtgatg
     4261 gctgatttgc gtcagactat tccaggattt agagaggacc atgcgaccat tgtccatgga
     4321 gatgtacgac atagtaattg gattgagaca gatagtggct tgatttattt agtagattgg
     4381 gattcggttc gcttgaccga tcgcatgttt gatgtggccc atatgctctg ccattatatt
     4441 tcagaacatc agtggaagga atggttgacc tactacggtt acaagtacaa tcaaacggta
     4501 ttaagtaaat tgtattggta tggtcaattg tcttatttga gtcagatttc caagtattat
     4561 atgaaccaag atttagaaaa tgtcaatcgg gagattcatg gtttgcgtca tttccgagac
     4621 aagtatggaa agagaagatg agagttagaa atcgtaaagg ggcaacagaa ttactagagg
     4681 caaatcccca gtatgtggtc ctcaatccct tggaagccaa ggcaaaatgg cgggacttgt
     4741 ttggcaatga taatcccatt catgtggaag ttggaagtgg aaagggtgcc tttgtttcag
     4801 gtatggccaa gcaaaaccct gacatcaact atatcgggat tgatattcaa aagtctgttt
     4861 tgagctacgc tttggacaag gtgcttgaag ttggagtgcc taacatcaag ctcttgtggg
     4921 tagatggttc tgacttgact gactactttg aagacggtga gattgatcgc ttgtatctga
     4981 acttttcaga tccatggccg aaaaaacgcc atgaaaagcg tcgtttgacc tacaagacct
     5041 ttttggatac cttcaaacgt atcttgcctg aaaatggaga aattcatttc aagacggata
     5101 accgtggctt gtttgagtac agtttagtga gcttttctca atatggcatg aaactcaatg
     5161 gtgtctggct agatttgcat gccagtgatt ttgaaggcaa tgtcatgaca gaatacgagc
     5221 aaaaattctc aaacaagggg caagttatct accgagttga ggcagaattt taagagatag
     5281 cctaaaatta ggctgtacaa gtgcttttgc tttacataag ttggcaaacg tgctatactg
     5341 atagtaagaa tatgaaaagt gaggcgggga aatatcttcg cctcttgctt atgaggaggt
     5401 ggacgcaatc gcaacaatcg tagaattagt cagagaagtt gtagaacctg tcatagaagc
     5461 tccttttgaa ctcgtggata tcgagtatgg aaagattggc agtgacatga ttctcagtat
     5521 ttttgtagat aaacccgaag gaattacctt gaacgacacg gcagacttga cagaaatgat
     5581 cagtcctgtc ctagacacca tcaagccaga tcccttccca gaacaatatt tcctagaaat
     5641 taccagtcca ggtttggaac gtcctttgaa aaccaaggat gccgtcgctg gagcggttgg
     5701 aaaatacatc catgtcgggc tctaccaagc catcgataag caaaaggtct ttgaaggaac
     5761 cttgttggcc ttcgaagagg acgagttgac tatggaatat atggacaaga cgcgtaagaa
     5821 aaccgtccaa attccataca gtttagtatc aaaagcacgt ttagcagtta aattatagaa
     5881 aaagaaagga tagcttttga ggattcaaaa gtgaagaaaa catgagtaaa gaaatgctag
     5941 aggccttccg cattttggaa gaagacaagg gaatcaaaaa agaagatatc atcgacgcag
     6001 tagtagagtc gcttcgttcc gcttatcgca gacgctatgg tcagtcagac agcgtagcta
     6061 ttgacttcaa cgaaaaaaca ggtgacttta cagtttatac tgtccgtgaa gttgttgatg
     6121 aagtatttga tagccgtttg gaaatcagct tgaaagatgc tcttgccatt aattcagctt
     6181 atgaacttgg agacaaaatc aagtttgaag aagcaccagc tgagtttggt cgtgtagcag
     6241 cccaatctgc caaacaaacc atcatggaaa aaatgcgcaa gcaaacacgt gccatcactt
     6301 acaatactta caaagaacat gagcaagaaa tcatgtctgg tacagtagaa cgctttgaca
     6361 accgctttat ctatgtcaac cttggtagca tcgaagccca attgtcaaaa caagaccaaa
     6421 ttcctggaga agtttttgct tctcatgatc gtatcgaagt ttatgtttac aaggttgaag
     6481 acaaccctcg tggtgtgaac gtctttgtta gccgtagcca tccagaaatg atcaaacgtt
     6541 tgatggagca agaaattcca gaagtttatg atggaactgt tgaaatcatg agcgtggctc
     6601 gtgaagcagg tgaccgtacg aaggttgctg ttcgtagcca caatccaaac gtggatgcta
     6661 tcggtacaat cgttggacgt ggtggtgcta atatcaagaa gattactagc aaattccacc
     6721 cagctcgtta cgatgctaaa aatgaccgca tggtaccaat cgaagaaaat atcgatgtta
     6781 tcgagtgggt agcagatcca gctgaattta tctacaatgc catcgctcct gctgaggttg
     6841 accaagttat ctttgatgaa aacgacagca aacgtgcctt ggtggttgtt ccagataaca
     6901 agctttctct tgccattggt cgtcgtggac aaaacgtgcg cttggcggct cacttgactg
     6961 gttaccgtat cgatatcaag tctgctagcg aatttgaagc catggaagac gctgcttcag
     7021 tagagttgga agtagaaaac gatactgtag aagaataaaa gctgctagag gagggaaaga
     7081 tgaaaacaag aaaaatccct ttgcgcaagt ctgttgtgtc taacgaagtg attgataagc
     7141 gtgatttgct ccgcattgtc aagaacaagg aaggacaagt ctttattgat cctacgggca
     7201 aggccaatgg ccgcggcgct tatatcaaac tagacaatgc agaagcccta gaggcgaaaa
     7261 agaagaaggt ctttaaccgc agctttagca tggaagtgga agaaagcttt tatgacgagt
     7321 tgatcgctta tgtggatcac aaagtgaaaa gaagagagtt gggacttgaa taagcaaaag
     7381 ataagtaatc tcttggggct tgctcagcga gcagggcgca tcatatcggg tgaagaattg
     7441 gtggtcaagg ccattcaaga cggcaaggcc aagttggtct ttctagctca tgatgctgga
     7501 cccaatctgg ccaagaagat tcaagataaa agtcattatt atcaagtaga aattgtaacc
     7561 gtgttttcaa cactggaatt aagcatagca gtcgggaaat cgagaaaggt tttggctgta
     7621 acagatgctg gatttacaaa gaaaatgagg tctcttatgg aatagaagag gaggacatga
     7681 tttgtctaag aaaagattgt acgaaatcgc aaaagaactt ggaaaagaaa gtaaagaagt
     7741 tgtagcgcgt gcaaaagagt tgggcttgga tgtgaaaagc cactcatcaa gtgtggaaga
     7801 agctgtcgct gcaaaaattg ctgccagctt taagcctgca gctgctccga aagtagaagc
     7861 aaaacctgca gcaccaaaag taagtgcaga aaagaaaacc gaaaaatctg agccagctaa
     7921 accagctgta gctaaggaag aggcaaaacc ggctgagcca gttgctccga aaacagaaaa
     7981 agtagcagcg aaaccgcaaa gccgtaattt caaggctgag cgtgaagccc gtgctaaaga
     8041 gcaggcagaa cgacgcaagc aaaataaggg caataaccgt gaccaacaac aaaacggaaa
     8101 ccgtcagaaa aacgatggcc gtaatggtgg aaaacaaggt caaagcaacc gcgacaatcg
     8161 tcgctttaat gaccaagcta agaagcagca aggtcagcaa aaacgtagaa atgagcgccg
     8221 tcagcaagag gacaaacgtt caaatcaagc ggctccacgt attgacttta aagcccgtgc
     8281 agcagcccta aaagcagagc aaaatgcaga gtacgctcgt tcaagtgagg aacgcttcaa
     8341 gcagtatcag gctgctaaag aagccttggc tcaagctaac aaacgcaagg aaccagagga
     8401 aatctttgaa gaagcggcta agttagctga acaagcacag caagttcaag cagtggttga
     8461 agtcgtccct gagaaaaaag aacctgcagt ggatacacgt cgtaaaaaac aagctcgacc
     8521 agacaaaaat cgtgacgatt atgatcatga agaagatggt cctagaaaac aacaaaagaa
     8581 tcgaagtagt caaaatcaag tgagaaatca aaagaatagt aactggaata acaacaaaaa
     8641 gaacaaaaaa ggcaataaca agaacaaccg taatcagact ccaaaacctg ttacggagcg
     8701 taaattccat gaattgccaa cagaatttga atatacagat ggtatgaccg ttgcggaaat
     8761 cgcaaaacgt atcaaacgtg aaccagctga aatcgttaag aaacttttca tgatgggtgt
     8821 catggccaca caaaaccaat ccttagatgg ggaaacaatt gaactcctca tggtggatta
     8881 cggtatcgaa gccaaacaaa aggttgaagt ggataatgct gacatcgaac gtttctttgt
     8941 cgaagatggt tatctcaatg aagatgaatt ggttgagcgt ccaccagttg ttactatcat
     9001 gggacatgtt gaccacggta aaacaaccct tttggatact cttcgtaact cacgtgttgc
     9061 gacaggtgaa gcaggtggta ttactcagca tatcggtgcc taccaaatcg tggaaaatgg
     9121 taagaagatt accttccttg atacaccagg acacgcggcc tttacatcaa tgcgtgcgcg
     9181 tggtgcttct gttaccgata ttacgatctt ggtcgtagcg gcagatgacg gggttatgcc
     9241 tcagactatt gaagccatca accactcaaa agcagctaac gttccaatca tcgtagccat
     9301 taacaagatt gataaaccag gtgctaaccc agaacgcgtt atcggtgaat tggcagagca
     9361 tggtgtgatg tcaaccgctt ggggtggaga ttctgaattt gttgaaatct cagctaaatt
     9421 caaccaaaat atcgaagaat tgttggaaac agtccttctt gtagctgaaa tccaagaact
     9481 caaggcagac ccaacagtgc gtgcaatcgg tacggttatc gaagcgcgct tggataaagg
     9541 aaaaggtgcg gtcgcaaccc ttcttgtaca acaaggtacc ttgaatgttc aagacccaat
     9601 cgttgtcgga aataccttcg gtcgtgtccg tgctatgacc aacgaccttg gtcgtcgtgt
     9661 taaagttgct ggaccatcaa caccagtctc tatcacaggt ttgaacgaag caccgatggc
     9721 gggtgaccac tttgccgttt acgaggatga aaaatctgcg cgtgcagcag gtgaagagcg
     9781 tgccaaacgt gccctcatga aacaacgtca agctacccaa cgtgttagcc ttgaaaacct
     9841 ctttgatacc cttaaagctg gggaactcaa atctgttaat gttatcatca aggctgatgt
     9901 acaaggttct gttgaagccc tttctgcctc acttcaaaag attgacgtgg aaggtgtcaa
     9961 agtgactatc gtccactcag cggtcggtgc tatcaacgaa tcagacgtga cccttgccga
    10021 agcttcaaat gcctttatcg ttggtttcaa cgtacgccct acaccacaag ctcgtcaaca
    10081 agcagaagct gacgatgtgg aaatccgtct tcacagcatt atctacaagg ttatcgaaga
    10141 gatggaagaa gctatgaaag ggatgcttga tccagaattt gaagaaaaag ttattggtga
    10201 agcggttatc cgtgaaacct tcaaggtgtc taaagtggga actatcggtg gatttatggt
    10261 tatcaacggt aaggttgccc gtgactctaa agtccgtgtt atccgtgatg gtgtcgttat
    10321 ctatgatggt gaactcgcaa gcttgaaaca ctataaagac gacgttaagg aagttacaaa
    10381 cggccgtgaa ggtggattga tgatcgacgg ctacaatgat attaagatgg atgatgtgat
    10441 tgaggcgtat gtcatggaag aaatcaagag ataagatttt ttgctccttt cttaggtggt
    10501 gagggacgca agcaaaccga tggtttcatt gcttattttt gagcctaggg tctcaaaaat
    10561 cccctgtgat gggactgata aatcagttcc atcactttca ccacggcgaa agaagcagat
    10621 gacttcaaat tgaacttcgt ttcaatttaa actgaaaatc aagaagttta aaatagctag
    10681 gtctgctggc ctagcttttg gttcaaagta gagaaaggaa tatcatggca aatcatttcc
    10741 gtacagatcg tgtgggcatg gaaatcaagc gtgaagtcaa tgagattttg caaaagaaag
    10801 tccgtgatcc acgtgtccaa ggtgtgacca tcacagatgt tcagatgctg ggtgacttgt
    10861 ctgttgccaa ggtttattac accattttga gtaaccttgc ttcggataac caaaaagccc
    10921 aaatcgggct tgaaaaagca actggtacca tcaaacgtga acttggtcgc aatttgaaat
    10981 tgtacaaaat cccagatttg accttcgtca aagacgagtc catcgagtat ggaaacaaga
    11041 ttgacgagat gctacgcaat ctggataaga actaaagaag aggggttgcc cctctttttt
    11101 ggtggaggaa aataggttga atttgaaatg gaaaaatatt cttttaaaat atattgaaac
    11161 tagaatagta c
//
LOCUS       DAVKJD010000058        10977 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKJD010000058 DAVKJD010000000
VERSION     DAVKJD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10977
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.058"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            11..733
                     /locus_tag="VW157_001535"
     CDS             11..733
                     /locus_tag="VW157_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9552813.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            730..1578
                     /locus_tag="VW157_001536"
     CDS             730..1578
                     /locus_tag="VW157_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9552814.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1610..2539
                     /gene="psaA"
                     /locus_tag="VW157_001537"
     CDS             1610..2539
                     /gene="psaA"
                     /locus_tag="VW157_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9552815.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2662..3153
                     /gene="tpx"
                     /locus_tag="VW157_001538"
     CDS             2662..3153
                     /gene="tpx"
                     /locus_tag="VW157_001538"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9552816.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3249..5978)
                     /locus_tag="VW157_001539"
     CDS             complement(3249..5978)
                     /locus_tag="VW157_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9552817.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQESLDKGQETLDKA
                     QTNLQEGKRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAE
                     QNLAQEKEKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPV
                     VLYAVAAMVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGS
                     ILGHYLLASVISSVITKGMVVGETQIQFYWTYSLLAFVLSLLASVLPAYLVAWRELHD
                     EAAQLLLPKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSV
                     ALLFAGLGIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQ
                     KIYSKALYKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQL
                     AGVKVGQTLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTS
                     ATSIESQAGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYN
                     LTNINVAERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLIAGFYLHQFLIQ
                     MISPATILFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5980..6681)
                     /locus_tag="VW157_001540"
     CDS             complement(5980..6681)
                     /locus_tag="VW157_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552818.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNSDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6931..9342)
                     /locus_tag="VW157_001541"
     CDS             complement(6931..9342)
                     /locus_tag="VW157_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9552819.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYGFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLIQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSEANKINLWARLGDGNRAHKLLAEQLKTSTLQNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9395..10087)
                     /locus_tag="VW157_001542"
     CDS             complement(9395..10087)
                     /locus_tag="VW157_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9552820.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10621..10977)
                     /locus_tag="VW157_001543"
     CDS             complement(10621..10977)
                     /locus_tag="VW157_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552821.1"
                     /translation="MSGIVGLVGGILLLIGPFVLLGTAVNTAATTLNGGATAGAFSGV
                     ALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGGVSLIPFLGWVGGILAII
                     GGSLFLATLKKFKSEE"
BASE COUNT         3198 a         2524 c         2057 g         3198 t
ORIGIN      
        1 atacaagttt atgatacgta tcgaaaacct cagtgtctcc tacaaagaaa cgttggcact
       61 taaggatatt tcactagtgc tccatggacc aacaattacc ggcatcattg gtccaaacgg
      121 cgctgggaaa tcaacactat taaaaggtat gctgggaatt atcccacatc aaggtcaggc
      181 atttctcgat gacaaggaag ttaaaaaatc cttacaccga attgcctatg tcgaacaaaa
      241 aatcaatatc gactacaact ttcccatcaa ggtcaaggaa tgcgtctcgt taggactatt
      301 tccctctatt cctctctttc gaagtttaaa ggctaaacat tggaagaaag tgcaagaggc
      361 ccttgaaatc gtcggcctag ctgactacgc tgaacgtcaa attagtcaac tgtctggagg
      421 tcaattccag cgggtcttga ttgccagatg tttggtgcag gaagccgact atatcctctt
      481 ggatgaaccc tttgctggga ttgactctgt cagtgaggaa atcatcatga atacgctgag
      541 agatttgaaa aaagctggga agacggttct catcgttcac cacgacctca gcaagattcc
      601 ccactacttc gatcaagtct tacttgtcaa tcgagaagtg attgcctttg gtccaacaaa
      661 agaaactttt accgaaacca atctaaaaga agcttacggt aatcaactct ttttcaatgg
      721 aggtgaccta tgattgcaga atttatcgat ggattgcaaa aattccattt cctacaaaat
      781 gccttgataa cagctattgt cgtcgggatt gtagctggag ctgtgggatg tttcattatt
      841 ctacgcggga tgtcactcat gggagatgcc atttcacatg ctgtcttacc aggtgtagcc
      901 ctctccttca tcttgggcct tgacttcttt atcggagcca ttgtctttgg attgctagct
      961 gccatcatca ttacctacat caaaggaaac tcgattatca aaagcgatac cgccatcggc
     1021 attacctttt cttctttctt agccctcggt atcatcttga ttggtgtcgc taaaagttca
     1081 actgaccttt tccatatcct ttttggtaat attctggccg tccaagatac ggatatgttt
     1141 attactatgg gtgtgggggc agccatcctc ttgttaattt ggattttctt caagcaactc
     1201 ttgataactt cctttgatga actcttggct aaagccatgg gaatgcctgt caatttctat
     1261 cactaccttc tcatggtact cctgactctc gtgtctgtga cagccatgca aagtgtcgga
     1321 actatcctga ttgtagccat gctgattacc ccagctgcaa ctgcttatct gtatgctaat
     1381 agtctgaaaa gcatgatttt cctttcctca accttcggag ctactgcttc agttttggga
     1441 ctctttattg gctatagttt taatgttgcg gcaggttcta gtatcgtgct tacagctgct
     1501 agtttctttc tcattagctt ctttatcgct cccaaacaac gatatttgaa actgaaaaat
     1561 aaacatttgt taaaataagg ggcaaagccc taataaattg gaggatctaa tgaaaaaatt
     1621 aggtacatta ctcgttctct ttctttctgc aatcattctt gtagcatgtg ctagcggaaa
     1681 aaaagataca acttctggtc aaaaactaaa agttgttgct acaaactcaa tcatcgctga
     1741 tattactaaa aatattgctg gtgacaaaat tgaccttcat agtatcgttc cgattgggca
     1801 agacccacac gaatacgaac cacttcctga agacgttaag aaaacttctg aggctgattt
     1861 gattttctat aacggtatca accttgaaac aggtggcaat gcttggttta caaaattggt
     1921 agaaaatgcc aagaaaactg aaaacaaaga ctacttcgca gtcagcgacg gcgttgatgt
     1981 tatctacctt gaaggtcaaa atgaaaaagg aaaagaagac ccacacgctt ggcttaacct
     2041 tgaaaacggt attatttttg ctaaaaatat cgccaaacaa ttgagcgcca aagaccctaa
     2101 caataaagaa ttctatgaaa aaaatctcaa agaatatact gataagttag acaaacttga
     2161 taaagaaagt aaggataaat ttaataagat ccctgctgaa aagaaactca ttgtaaccag
     2221 cgaaggagca ttcaaatact tctctaaagc ctatggtgtc ccaagtgcct acatctggga
     2281 aatcaatact gaagaagaag gaactcctga acaaatcaag accttggttg aaaaacttcg
     2341 ccaaacaaaa gttccatcac tctttgtaga atcaagtgtg gatgaccgtc caatgaaaac
     2401 tgtttctcaa gacacaaaca tcccaatcta cgcacaaatc tttactgact ctatcgcaga
     2461 acaaggtaaa gaaggcgaca gctactacag catgatgaaa tacaaccttg acaagattgc
     2521 tgaaggattg gcaaaataag cctctgaaaa acgtcattct catgtgagct ggcgtttttt
     2581 ctatgcccac atttccggtc aaatcattgg aaaattctga ctgtttcaga tacaatggaa
     2641 gaaaaaagat tggagtatcc tatggtaact tttctcggaa atcctgtgag ctttacaggt
     2701 aaacaactac aagtcggcga caaggcgctt gatttttctc ttactacaac agatctttct
     2761 aaaaaatctc tggctgattt tgatggcaag aaaaaagtct tgagtgtcgt tccttctatc
     2821 gatacaggca tctgctcaac tcaaacacgt cgttttaatg aagaattggc tggactggac
     2881 aacacggtcg tattgactgt ttcaatggac ctaccttttg ctcaaaaacg ttggtgcggt
     2941 gctgaaggcc ttgacaatgc cattatgctt tcagactact ttgaccattc tttcgggcgc
     3001 gattatgccc tcttgatcaa cgaatggcac ctattagcac gcgcagtctt tgtcctcgat
     3061 actgacaata cgattcgcta cgttgaatac gtggataata tcaattctga gccaaacttc
     3121 gaagccgcaa ttgcagctgc taaagcccta tagaaaaaat cctctgtcac aaagagttcc
     3181 ctgctctttg aaacggagga tttttgaata ttagtaaata gaagttcaat cagctaaaaa
     3241 taactacctt actctacaga tttcagggct tctaacatat caacctttct cagataataa
     3301 ttgacgaaga aaccaagcaa ggtcaaaatg atgcttactg ctgccactgg gattacatag
     3361 acttcccagc ctacctgcgg ataaaagaga atagtcgcag gcgaaatcat ttgaatcaaa
     3421 aattggtgta aatagaaacc agctatcaga ccaagtacga ttcccacaag ggacagcaca
     3481 atcgtctcac ggtaaatgta gagggtgact tcattattat gaaaaccaag aaccttgata
     3541 gtggagagtt cacggattct ctcagctacg ttgatattgg tcagattgta aaggatgaca
     3601 atagctaata gaaccgatac gatgaccaag atggtcatgg tctgattgag tgagctagcg
     3661 atagagtcga agagtcgaat ggctgaagca ttttggacaa cgctggacac cgcagattga
     3721 ttcataagca agcccgcctg actttcgata ctagttgcac tggtatccct taatgagacc
     3781 agataagtgt tggcttgggg tagctgtccg taaagttgct catagctagc ctgactcata
     3841 taaataaagt gaccaacgta gttctcagta atagcagcga cctttagttc cttaccttca
     3901 atttctaaag tctgcccaac cttgacacct gccagctggg cgagtttagc tgtaataacg
     3961 atgccatctt ttaatgtcag ctcctgctga tgatgttgaa gatggataaa gggagtcaaa
     4021 tcttccttct ctatcatcat aagagtaatg ttttgaagac cagctttgcc tttgaaatcc
     4081 ttgtatagcg ctttagaata gattttctgg taggctagta tctcctgccc tttcaacact
     4141 tctgctagct ctaccttgtc ctgattggtc gcactaggat tttcagagac aagcatctga
     4201 tactgttgga tttgttgaaa ctgtttagac ggaactcctg ctacagaaga ttggattccc
     4261 aaacctgcaa agagcagagc tacagaacct gccacaccaa agattgtcat caacattctc
     4321 tgcttataac gaaagatgtt gcgggctgtt accttatgag taaaactgag acgacgccag
     4381 ataaaaccga tacgctccaa taagatttta gctcctttga caggaggttt aggaagtaga
     4441 agctgggctg cttcgtcatg aagttccctc caagccacca gataggctgg taacacactc
     4501 gccaacaagc tcaagacaaa agctagtaag ctataggtcc aatagaactg aatctgagtt
     4561 tctcccacca ccatgccttt tgtaatgaca cttgaaatta cactggctag caaataatga
     4621 ccaagtatac tacctagagc cgttccgaca gtcccagcta ctagtccata aaggagaaac
     4681 ttggcgataa tatccttact acgataaccc aaggccttaa aaatccctgc atgagttcgc
     4741 tcttcgtcta caaagcgagt catggtcgta aaggtcacca tggctgctac ggcataaagt
     4801 accacaggaa agatattgcc cactgctcga atactggatg aagcattgct atacataaga
     4861 tagccctgac cacctggcat ggtctgacga ttatagacct gataccttgg ctccgccaaa
     4921 tcgtccaaga cttgctgatg tttttctaat ttttcctttt cttgggctag attttgttca
     4981 gcttgcttta gtttatcctc ttccttgccc aattcctgct tggcttgggt aagttgagca
     5041 ctagcctgct ctctctgaac ttgaggaaac aaggctagtt gactttcttg agcctgtata
     5101 cgagcttgag cagctgctaa acgacgcttg ccttcctgca aattagtctg agccttgtca
     5161 agggtctctt gccccttgtc tagagactct tgtcccttgt ctagagactc ttgtccttct
     5221 tttttcaaaa gttgcagacg taccttgcca ttatctgata aagtttgttc aaggtcttcc
     5281 tgatgttgct tggatttttc ttcataagct gatgaaaagg catttaaacc tgctaaatct
     5341 tgatatttca aacgagctat attgtagact ttctgatcaa actgactagg taaaatcaca
     5401 ccataggctg tcagagtccc acttccactt cctgcgtagc ccatatctcg ctgggagagg
     5461 atttcagccg aatccacaaa accagtaatg gtataagtat ggtcttttaa agaggaatga
     5521 ccctcttctt tttctttaaa actaatctcc tgtcccacgc tgtattggcc ttgcaaatga
     5581 gtggccaaag cgatttcctt gtctgactga ggaagtcgtc cctttcttag ctgaaaggtt
     5641 gaaattcgct ctggtttgga gtacagtcga atggcatcct gcccattatc catagtcaca
     5701 tctgtcaaat agccaaactc gacctctgcg ccctccgtct gttttagttc ttcttggtct
     5761 gcttgatcca agccatagtt agacatgact gccaaatcca aggtttgagc agttgttaaa
     5821 taagcattag ctgtcgcctc catgttggga ctggttactt tgaggcctac taaggctaga
     5881 gatcccaaca tcatcaggat caagatggat aaaaaacgcc ccttggagcc tgtgaaggac
     5941 tgaattaagt ccttccaata agtttttcgc ttgatcatac tagtactcca aactgtcaat
     6001 atcctgagga tgctggttga gcaccacatc cttgacactg gcatcgtgca tatgaatcac
     6061 gcgatcagca atgggcgcca aagctccatt atgagtcacg atgatcaccg tcgctccctt
     6121 ttgacgagac atgtcttgga gaattttcaa aacctgcttg cccgtctgat aatccaaggc
     6181 tccagtaggt tcatcacaaa gaagaatttt aggatttttg gctaccgcgc gtgcaatgga
     6241 gactcgctgt tgctcccctc cagaaagctg ggctggaaag ttatttagac gatgagccag
     6301 acctacatct gtcaagacct gatcagaatt caaggcatct gtcacaattt cagaagccag
     6361 ttccacattt tccttagctg tcagattaga aactagatta taaaactgaa aaacaaaccc
     6421 cacatcattt ctacggtaat tggtgcgctg gtgggaacta taatccgcaa tattaacacc
     6481 atcaatccag atttcccctt catcattggt atccattccc ccaagaaggt taagaactgt
     6541 tgacttgcct gcacctgaag caccaaggat aataaccagt tccccctttt caatctcaaa
     6601 attcacatca cgattggcca caatctccgt gtccccaacc tgataacgct tgtaacagtg
     6661 tttcatctca atataagcca tcagacccac cctctatcaa acaaattata ctcctcgaaa
     6721 atctctcttc aaaccacgtc agcttccatc tgcaacctca aaacactgtt ttgagcaacc
     6781 tgcggctagc ttccttgttt gtgctctttg attttcattg agtattactt cctattatca
     6841 ttataacgct ttttcaacta gttggaaact gcctatattc ttaaatcctt attaaaaggc
     6901 agtttcaaat tactgcctta taacatcttc ttaaaaataa aattgaacaa gatcaccttc
     6961 tgctgttgcc accgaaatac aatctttccc catgcatttc gcttttattt tttcttgatt
     7021 cattttaatc acactcttct caatatctgg ataagaaact cgcaaatctc ctccactcct
     7081 tgataaaatg gtcaactgta agagtttttt atcttcccag ctcatgctca cttcaaaatg
     7141 tccacgtgcc attaagcctg aaacagaacc tgttgaccaa gcatcaggta gggcagctag
     7201 aggtaccaga taagctgcat gagactggag taacatttct gccatgccac tagtagcacc
     7261 aaaattacca tctatctgaa aaggaggatg gctacaccaa agattttgca aggtggatgt
     7321 ctttaactgc tctgccaata atttatgggc tcgattgcca tctcccaaac gcgcccagag
     7381 attgatctta ttagcctcgg accagcctgt gccgccatct ccacgatcat tgaggctagc
     7441 acgcgccgct tcaatatact cttgtccctt gtagctaaag agattgccag gatagagtcc
     7501 cactagatgg gaagcgtgcc gatgctgggc ctccactttc tcattttgaa aatactgctc
     7561 ttcctcctca taccactccc tgattcgacc agattgagtg atttgcaaag gatttagtaa
     7621 atcagacttc tccttaacct cagtcaacaa gtcctcatcc agtcccaatt cctgagcagc
     7681 ctgaataaaa tcatgaaata actgccaaat cagagattgg tcataggtat tgccaatcga
     7741 aatcggccca tgttctgggg aataagacgg agaagacacc caacgctgcg cctgctgatc
     7801 cttatgtaaa aaggcattcc aaaaacgaac cgtttccctc aacatgggat aaattttctc
     7861 cctgagatag tcttggtccc tataaaatga ataggcttca taaacggttt gcatcatcca
     7921 cgcattggca gctggtgacc aaccccaata gtaatcccaa ccaggtgccg tccaaccaaa
     7981 gggagtcgct tgagtatgaa ccaaccaacc attctcctca cctttctgag agacgattcc
     8041 tgcatacttt acagccgcta gacgaccata gacacgcaaa tcatctacat agttgatgac
     8101 tggaaagacc gtctctagga gattggtaac ataggctggc caataattca tctgcagatt
     8161 gacatttaag tgatagtccg aattccaagg aggattgtcg accgcattcc agactccctg
     8221 taggttagct ggtagagcat ctgggcagtc tctggacgaa ctaatcaata aataccgtcc
     8281 atactggaag aacagctcct ccaaagcctg cccttcttgt ggcttataat tttttaacaa
     8341 atcatctgta gtggatgcgt caacatcagc ttccaaatcc aattgaacac gctggaataa
     8401 ggcttggtag tcctcgatat gccttgattt caattgggta tagccctttt ctttagctgt
     8461 gtccaccaag tctatcacct gttgctctaa atctagtttc ttgcgataat tgctagcagg
     8521 attttgggca aaatccgtct tagcggccaa gaagagattg gcataactgg ctcctgatat
     8581 ctgaacccta tctgaccaaa ctctaatatc tccatccgtt tcccaagcta gataactagc
     8641 aaaccgcaga tcattatcct taactcttcc cttcatcaag atatgagaat cagtaatatc
     8701 caacttacac tccttgtaat cagatttttc ctgctcatac tttccatcag aagccaaatc
     8761 acaggtcaag gatagttcta tagtaaaatc tagagtttcc aacccttcct tagtaaaaca
     8821 ttgaatcaag agatcatctg gaaaactcgc aaaagcttta cgttcaaatc gcgttccctt
     8881 atagacataa gaagtcgtcg caagtgcctt actaatattc agctgtctct gatagtccgt
     8941 cacctgagac aaagtcgtac cttgctggct gaactcaatg tgaatatccc caaaagacag
     9001 ataggtccca tattgactcg tttttggccc aattaggtgc tgctcagcca gttccttagc
     9061 cagattgtaa tctctcttct ccaaagcctg ccgaatctca gctaaaaaac catactgatc
     9121 ctgaagattt ccaccctgat aatctgaact atcaggaagt ggacctccag accagagact
     9181 tttttcatta aattgaatcc gttcagcccc tataaggcca aatacttttg ctcctaaaga
     9241 accattgcct atcggtaaag cttcttcttc ccaacccatg taagtggttg aagctggttg
     9301 cttgtaggcc agtacataat cttgtttttt attccttatc atacttccat actaacataa
     9361 aaagcctaga aatcaattct agacttacaa ttttttattt tccaaggtag tattcagaaa
     9421 cgacgttcaa tttttcgtcg aattcgaata ccaatggtgg gaagttaggg atttccacgt
     9481 ccatgatctc gtcatctgac aaacctttga tgtgttttac aagggcacgg attgagttac
     9541 cgtgagctcc tacgaatacg tttttaccat ctttaagagc tggagcgatt ttatcttccc
     9601 agaatggaag agcacgttcc aaagtcactt tcaagttttc agcatctggg ataactgagt
     9661 cgtcaagtga agcgtaacga cggtctgtgt gagctgagtg ctcatcatca cggtccatgt
     9721 ttggaggcaa tacatcgtat gaacgacgcc agatgtgaac ttgctcatca ccaaattgtt
     9781 cagcagcttc agctttattt ttaccagtca aaccaccata gtgacgttcg ttcaagcgcc
     9841 atgatttttc aactggaacc cacaattggt cagaagcttc aagagccaag ttagttgttt
     9901 tgatagcacg tttcaatact gaagtgtaag cttggtcaaa tttgatacca gcttctttga
     9961 tcaatttacc agcgtcaatc gcttgttgtg tacctttttc agacaaatca acatcagccc
    10021 aaccagtgaa aaggttagct ttgttccatt cagactcacc gtggcgagca aaaaccaatt
    10081 ttaccattag atggattctc ctttaaattt tccgaggttt cccctcgttt atctattcta
    10141 ttttacacaa tttttcacaa aaaagctagt caaaaccaaa aaggaaagga ctgtttgaca
    10201 atgtttggca atcacattgt ttttggttgt ttctattcta tcttcattat ttaacctttt
    10261 tttctgcgca tcgaataatt tttgagttga ctcttttagt ttagtaaacg agtcattatt
    10321 ttccatagct gtgcctttga aaccttcctc agctttagta aaataatcat caaaaatatt
    10381 tttataaaga tctatataat ctgcataagt gcgtattgat tcaatcgcat cgtctgttac
    10441 aaccggtgta tcgaaagtga ttgtttcttt tgaactattg gatgttgcta ctgtcttctc
    10501 atcattactt gttgaagtat tctttgaact ttatgttcca catgcaacta gaagagaaag
    10561 agataataga gctactccta taccgataaa ctttttgttc ttttcatgct aaaatacctt
    10621 ttattcttct gatttgaatt tcttcaatgt tgcaaggaat agagatcctc cgataatagc
    10681 aagaatcccc ccaacccatc ctaagaatgg aatgagacta actccaccag aaacaatcat
    10741 tagtacagac ggagctgcac ctacacgctt atctccttta tagtaaacaa tagcaatgat
    10801 accaagaaca agatttgcaa tcttcaaggc attcaagagt aaggctacac ctgaaaaagc
    10861 ccctgcagta gctcctccat taagagttgt agcagctgtg tttaccgctg ttcccaacaa
    10921 gacaaaagga ccaataagaa gtaaaattcc tcccacaaga ccgacgatac cactaat
//
LOCUS       DAVKJD010000059        10777 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKJD010000059 DAVKJD010000000
VERSION     DAVKJD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10777
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.059"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            132..629
                     /locus_tag="VW157_001544"
     CDS             132..629
                     /locus_tag="VW157_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9552822.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            629..2284
                     /gene="dnaX"
                     /locus_tag="VW157_001545"
     CDS             629..2284
                     /gene="dnaX"
                     /locus_tag="VW157_001545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9552823.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKSEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2312..2506
                     /locus_tag="VW157_001546"
     CDS             2312..2506
                     /locus_tag="VW157_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9552824.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2656..3426
                     /gene="sufC"
                     /locus_tag="VW157_001547"
     CDS             2656..3426
                     /gene="sufC"
                     /locus_tag="VW157_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9552825.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3454..4716
                     /gene="sufD"
                     /locus_tag="VW157_001548"
     CDS             3454..4716
                     /gene="sufD"
                     /locus_tag="VW157_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9552826.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4727..5953
                     /locus_tag="VW157_001549"
     CDS             4727..5953
                     /locus_tag="VW157_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9552827.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAIEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5940..6380
                     /locus_tag="VW157_001550"
     CDS             5940..6380
                     /locus_tag="VW157_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9552828.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6434..7846
                     /gene="sufB"
                     /locus_tag="VW157_001551"
     CDS             6434..7846
                     /gene="sufB"
                     /locus_tag="VW157_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9552829.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8077..9318)
                     /locus_tag="VW157_001552"
     CDS             complement(8077..9318)
                     /locus_tag="VW157_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9552830.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9421..10530
                     /locus_tag="VW157_001553"
     CDS             9421..10530
                     /locus_tag="VW157_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9552831.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3204 a         1975 c         2335 g         3263 t
ORIGIN      
        1 tacacaaaaa taggctccat aatatctata gtggatttac ccactacaaa tattatagag
       61 ccaaaaatcc tttgtttact aaacaaggga tttttctttt gtctctgctc cttttttgat
      121 ataatagttc tatgttaaaa tcagaaaaac aatcacgtta tcaaatgtta aatgaagaat
      181 tgtccttcct attggaaggc gaaaccaatg ttttggctaa tctttccaac gccagtgctc
      241 tcataaaatc acgttttcct aataccgtat ttgcaggctt ttatttgttc gatggaaagg
      301 aattggtttt aggccccttc caaggaggtg tttcctgcat ccgtattgca ctaggcaagg
      361 gtgtttgtgg tgaggcagct cactttcagg aaactgttat tgttggagat gtgacgacct
      421 atctcaacta tatttcttgt gatagtctag ctaaaagtga aattgtggtg ccgatgatga
      481 agaatggtca gttacttgga gttctggatc tggattcttc agagattgag gattacgatg
      541 ctatggatcg agattatttg gaacaatttg tcgctatttt gcttgaaaag acagcatggg
      601 actttacgat gtttgaggaa aaatcttaat gtatcaagca ctttatcgaa aatatagaag
      661 tcaaaacttc tcccagttag ttggtcaaga agttgtggct aagactctta aacaagcggt
      721 ggagcaagag aaaataagtc acgcttatct tttttctggt cctcgtggaa cgggaaaaac
      781 cagtgttgct aaaatctttg ccaaggctat gaactgtccc aatcaagtgg gtggcgaacc
      841 ttgcaataac tgctatattt gtcaagcagt gacggacggt agtttagaag atgtcattga
      901 aatggatgca gcttctaata atggggtaga tgaaattcgc gaaattcgtg ataaatctac
      961 ctatgcgcct agccttgctc gttataaggt ttatatcata gatgaggttc acatgctgtc
     1021 tacaggggct tttaatgccc tcctaaagac gctggaagaa ccaacacaga atgtagtctt
     1081 tattttggcc actactgaat tgcacaagat tcctgctact attctatccc gtgtgcaacg
     1141 ttttgagttt aaatcaatta agacacagga tattaaggaa catattcact atatcttaga
     1201 aaaagaaaat atcagttctg aaccagaggc tgtggaaatc attgccagac gggcggaagg
     1261 tggaatgcgg gacgccttgt ctattttgga tcaagccctg agtttgacac agggaaatga
     1321 gctgacgact gctatctctg aagaaattac tggcaccatt agcctatcag ccttggatga
     1381 ttatgtggcg gccttgtctc aacaggatgt tcccaaagct ttgtcttgct tgaatcttct
     1441 ttttgacaat ggtaagagca tgactcgttt tgtgaccgat cttttgcact atttaagaga
     1501 cttgttaatt gttcaaacag ggggagaaaa tactcatcat agttcagtct ttgtagaaaa
     1561 tttggcactt cctcaaaaaa atctgtttga aatgattcgc ttagcaacag tgagtttagc
     1621 agatattaag tctagtttgc agcccaagat ttatgctgaa atgatgaccg tccgtttggc
     1681 ggaaatcaag tccgaaccag ctctatcagg agcggttgaa aatgaaattg ctacgctgag
     1741 acaggaagtt gcccgtctca aacaagagct ttctaatgta ggtgcggttc ctaaacaagt
     1801 tgcaccagct cctagtcgac cagctacggg caaaacagtc tatcgtgtcg atcgcaataa
     1861 agtgcaatct atcttacaag aggccgtcga aaatcctgat ttagcacgtc aaaatttaat
     1921 tcgtttgcag aatgcctggg gagaggtaat tgaaagtcta ggtgggccgg acaaggctct
     1981 gctagttggt tctcaaccgg ttgctgccaa tgaacaccat gctattcttg cttttgagtc
     2041 taacttcaat gctggtcaaa ctatgaaacg agacaatctc aataccatgt ttggtaatat
     2101 cctcagtcag gcggcaggtt tttcacctga gattttagct atttccatgg aggaatggaa
     2161 agaagttcgc gcagcctttt cagccaaagc caaatcttct caaactgaaa aagaagtaga
     2221 agaaagcctg attccagaag gatttgaatt tttggctgat aaagtgaagg tagaggaaga
     2281 ctaaagaaag atttcatgat acaataagtt tatgaataaa caacaattta ttattatggc
     2341 gctgtttaca gctgctgaga cctatttttt caatgaggcc tggatgactg gccgctatat
     2401 tatggcagcc ttttgggcaa ttttactctt tagaaatttc cgagtcagtt atgtgatggg
     2461 caaaatcgtt gatgtcatcg atcagcattt taataggaaa gactagccct cagcttccag
     2521 acaaaatcaa agccttttag gctttttttt gttatactag aaaagtatat ttatagaatt
     2581 tttgctctat ttctggggaa atcagacgtt tttctagtaa gtactgtaaa agttttgaaa
     2641 aagaaaggaa ctatcatgtc agtattagag atcaaagatc ttcacgttga gattgaagga
     2701 aaagaaattt taaaaggggt taacctgacc ctgaaaacag gagaaattgc cgctatcatg
     2761 ggaccaaatg gtacaggtaa atcgactctt tctgccgcta tcatgggaaa tccaaactat
     2821 gaagtaacta aaggtgaagt tttgtttgat ggcgtaaaca tccttgagtt ggaagtggat
     2881 gagcgtgcgc gtatgggact tttccttgct atgcaatacc catcagaaat ccctggaatt
     2941 accaatgctg agtttcttcg tgccgctatg aatgcgggta aagaagatga tgagaagatt
     3001 tcagttcgtg agtttattac taagctagat gaaaaaatgg aattgcttaa catgaaagaa
     3061 gaaatggcag agcgttacct caacgaaggc ttctctggtg gtgagaaaaa acgcaatgaa
     3121 attcttcaac ttttgatgtt ggagccaaca tttgctcttt tggacgagat tgactcaggt
     3181 cttgatattg acgctcttaa agttgtgtct aaaggtgtca atgccatgcg tggtgaaggt
     3241 tttggtgcta tgatcatcac tcactaccaa cgtcttttga actatatcac acctgatgtg
     3301 gtacacgtga tgatggaagg tcgtgttgtc ctttctggtg gtccagaatt ggctgcgcgt
     3361 ttggaacgtg aaggatacgc aaaattagct gaagaacttg gctacgacta caaggaagaa
     3421 ttgtaattcc ctcgtatctt ttaggagaag taaatgacta gagaaaatat taaacttttt
     3481 tcagaaatgc acgctgaacc aagctggttg gctgatctcc gtcaaaaagc ttttgacaag
     3541 attgagactt tggaattacc agttattgag cgtgtcaaat tccaccgttg gaatctgggt
     3601 gatggaacga ttacagaaaa tgagccatca gcaaatgttc cagatttcac agctttagat
     3661 catcacttga agttggtgca agtaggaact caaactgttt tcgaacaaac tccagttgag
     3721 ttagctgaac agggtgttgt cttcacagac tttcactcag ctttagaaga aattccagag
     3781 ctgatcgaag aattcttcat gtcatctgtt aagtatgatg atgacaagtt ggcggcttac
     3841 cacacagctt actttaacag tggtgctgta ctctatattc cagataacgt agaaatcaca
     3901 gagccaattg aaggaatttt ctaccaagat agcgatagca atgtgccgtt taacaagcat
     3961 attatgatta tcgctggtaa aaattctaag attagttatc tggagcgttt agagtcacgc
     4021 ggtgaaggaa gtgacaaagc aactgccaat atcacagtgg aagtgattgc acgttctggt
     4081 gcgcaagtca agtttgctgc tatcgaccgt ctaggtgaaa acgtcactgc ctacattagc
     4141 cgtcgtggta aattaggcaa cgatgcaagt attgactggg ctatcggtgt catgaacgaa
     4201 ggaaatgtcg ttgctgattt tgatagtgac ttgattggta atggtagcca tgctgacctc
     4261 aaggttgtag ctctttcaag tggtcgtcag gtacaaggga ttgatactcg tgtaactaac
     4321 tatggctgca actcaatcgg aaacattcta caacatgggg ttatccttga aaaagcaact
     4381 ttgactttca atggtatcgg ccacatcatc aagggtgcta agggagcaga tgcgcaacaa
     4441 gagagccgtg ttctcatgct ttcagaccaa gcgcgttcag atgctaaccc aattcttttg
     4501 attgatgaaa atgacgtaac tgcaggccat gcagcctcta ttggtcaggt agatccagaa
     4561 gatatgtact acctcatgag tcgtggcttg gataaggcaa ctgcagagcg tttggttgtt
     4621 cgtggtttcc ttggatctgt tatcgtggag attccagtca aggaagttcg tgatgaaatg
     4681 attgcaacta tcgaagagaa attgtcaaaa cgctaagggg cagcctatgt tagatgtaga
     4741 agcgattcgc aaggattttc caattttaga tcagattgtc aatgatgaac ctctggtcta
     4801 tctggacaat gctgcgacga cacaaaaacc actagtagtt ctgaaagcta ttaacagcta
     4861 ctatgagcag gacaatgcca atgttcaccg tggtgtccat accttagcgg aacgagcgac
     4921 agcttcttat gaagctgctc gtgaaaccat tcgtaagttt attaatgcag gctctacaaa
     4981 ggaagttctc tttaccagag gaacgacaac cagccttaac tgggtggcac gctttgctga
     5041 ggaaattctc actgagggag accaggtctt gatttcagta atggaacacc attctaatat
     5101 cattccatgg caggaagctt gtcgaaagac tggagcagag cttgtctatg tctatcttaa
     5161 agacggtgcc ttggatatgg aggatttgcg agctaaattg actgataagg ttaaatttgt
     5221 ttccctagct catgcctcca atgttcttgg tgtggtcaat ccgatcaagg aaatcactca
     5281 attagcccac caagttgggg caatcatggt agtggatggt gctcaatcta cacctcatat
     5341 gaagattgat gtccaggact tggatctgga ctttttcgcc ttttcgggtc acaagatggc
     5401 tggtccgact ggtatcggtg tcctttacgg caaagaaaag tatcttgagc aaatgtctcc
     5461 agtagaattt ggcggcgaga tgattgattt tgtctacgag caatctgcta gttggaagga
     5521 attgccttgg aaatttgagg ctggaacgcc aaatatggca ggagctattg gacttgcgac
     5581 tgcagttgat tatctggaaa agattggtat ggatgccatt gaagctcatg aacaggaatt
     5641 gattgcgtac gtctatccaa aactgcaggc aattgaggga ttgaccattt acggttctca
     5701 ggatttggct caacgttcgg gtgttattgc ctttaaccta ggtgatctcc atcctcacga
     5761 tcttgcgacg gctctggatt atgaaggagt ggctgttcgt gctggtcacc attgtgcgca
     5821 acccttgctt cagtatttgg aagtcccagc aacagctcgt gcaagttttt atatctacaa
     5881 taccaaggca gattgcgaca aactagtcga tgccctacaa aagacaaagg agtttttcaa
     5941 tggcactttc taaactagat agcctttata tggcagtggt agcagaccat tcgaaaaatc
     6001 cacatcacca agggaagtta gaagacgctg agcaaatcag tctcaacaat ccgacttgtg
     6061 gggatgtcat caacctctct gtcaagtttg atgcagagga ccgtttggaa gatattgctt
     6121 ttctaaattc aggatgcacg atttcaactg cttctgctag tatgatgaca gatgccgttt
     6181 taggaaaaac caaacaagaa attttagaac tggcgactat tttttctgaa atggttcaag
     6241 ggcaaaaaga tgagcgtcaa gaccaacttg gagacgcggc attcttgtca ggtgttgcca
     6301 aattccctca aagaatcaag tgtgcaaccc tagcttggaa tgcccttaag aaaacaattg
     6361 aaaatcaaga aaaacagtaa gacaagtttc ttttgtctta tgaattatta gaaatgaaga
     6421 aagaaaggat actatggctg aagaaagagt agaaccaaaa ccaattgacc ttggtgaata
     6481 taaatttggt ttccatgacg atgtagagcc tgtcttatcg acaggaaaag gactcaacga
     6541 aggtgttatt cgtgaattat ctgctgctaa gggtgagcct gagtggatgt tggagttccg
     6601 tttgaagtct tatgaaacct tcaaaaaaat gcccatgcaa acttggggag cagacttgtc
     6661 agagattgac tttgatgact taatctacta ccaaaaacca tctgacaaac cagcccgttc
     6721 ttgggatgat gtacctgaaa agattaaaga aacctttgaa cgtatcggga ttccagaagc
     6781 tgaacgtgct tatttagcag gggcttctgc ccagtacgag tcagaagtgg tttaccacaa
     6841 catgaaggaa gagttccaaa aattaggtat tatctttaca gatacagatt ccgcactcaa
     6901 ggaataccca gacttattta aacaatactt tgcgaagttg gtaccgccga cagataacaa
     6961 gttggcagcc ctcaactcag cagtatggtc gggtggaact tttatctacg tgccaaaagg
     7021 tgtcaaggta gatattccac ttcaaactta tttccgtatc aataacgaaa atataggtca
     7081 gttcgaacgt accttgatta tcgttgatga gggagcaagc gtccactacg tagaaggatg
     7141 tacagcacca acatattcaa gcaatagctt acacgctgcc attgtagaaa tttttgcttt
     7201 ggacggagct tatatgcgtt atacaactat ccaaaactgg tctgataacg tctataactt
     7261 ggtaacaaag cgtgctaagg ctcaaaagga tgccactgtt gagtggattg atggaaactt
     7321 gggtgccaaa acgactatga aatatccatc tgtttacctt gatggagaag gagcgcgtgg
     7381 taccatgctc tctatcgcct ttgctaatgc agggcaacac caagacacgg gtgctaagat
     7441 gattcacaat gctccacata ccagctcgtc tattgtgtct aaatccatcg ctaaaggtgg
     7501 aggaaaggtt gactaccgtg gacaagtcac ctttaacaag aactctaaga aatctgtttc
     7561 ccacattgaa tgtgatacca ttatcatgga tgacttgtca gcatcagata ctattccatt
     7621 taatgaaatt cacaactcgc aagtggcttt ggaacacgaa gccaaagtat ctaagatttc
     7681 agaagagcaa ttgtattatc tcatgagccg tggattgtca gaatctgagg caactgaaat
     7741 gattgtcatg ggatttgtag aaccctttac aaaagaactt ccaatggaat acgcagttga
     7801 gctgaaccgc ttgattagct atgaaatgga gggatcagtt ggataaaatt tgattttata
     7861 ctcttcgaaa atctcttcaa accacgtcag catcgcctta ccgtatgtat ggttactgac
     7921 ttcgtcagtt tcatctacaa cctcaaaaca gtgttttgag caacctgcgg ctagcttcct
     7981 agtttgttct ttgattttga gtattagatt tactcaaaat caaggatttt gaagatgaac
     8041 ttgtatcaaa aaatcgcggt ttaaaatcgc gattttttat aatttctcgt taacaaagcg
     8101 gacaaactga ttccaccaaa cttttaagaa gaaggctttt tcaattttct tgtctgctac
     8161 catttcgaaa ctagggcgct ctgtggtgat gtaaccttga ccaatcaagt ccttgtcttc
     8221 ataagtcaaa tggccaacca ctgttccagc ttcaagtggt gctgggattg ctttggaatc
     8281 aggtgtgaat tgaacagatt gggaagattg attcccaaca cgttctatta gatagatatc
     8341 ctctggagcc actgcagtta ctgtatcttc ttttccatct tgtacaggag ctttgctatc
     8401 ttgataggca tcgccttgtt gaacgatttt gcgaagtgta aatgtagaag aaatataatc
     8461 cattagggaa gatgtagctg taaatcgagc gtaaggatta ttgtcttgat gatctgcatt
     8521 taaaacaact gtgatgactc tcatgccttt ttcgacagta gtaccaacaa aagactctcc
     8581 agccttatct gttgttcctg tttttagccc atcaaaacca ccacggtaag caggcatacc
     8641 ttctaacatg tagttggttg aagtgattgt catcccagca aaagtagaag aaggtttttt
     8701 ggtgatttct aagacttgtg ggtatttttt gatgaggttg cgagcaacga tagcgacatc
     8761 ataagcacta agcttatttt cctcatcttt tttagaacct gggtaaatgt tatcccctag
     8821 agtttcattg ttaagacctg tcgtattgac aacagtggca tcctgaattc cccattccaa
     8881 gagttttgcc cgcatcatat cgacgaaatc tttttctgag ccagcaattt tctcagctag
     8941 ggcaatagcg gcgctgttgg cactagatac cagagttgct tcaagcaact cttcgacagt
     9001 ataattacgg gcctccatag gaatattact ggcttcagaa tttgtcgtca attgataagg
     9061 ataatcagaa atatctacag gagtggagag ggtaatactt ccgttttcca aagcttcata
     9121 gaccagataa acagtaatca attttgttat ggaagcaatt tcgacaggtt gcgttgcatc
     9181 cttctcatag agaattttac cagtatttgc ctcaacagca atcgcatgtt tagcggcaat
     9241 ggtaaaatct tgagcaacag cagtagaagc accccctaaa agagagacag ttaacaaagt
     9301 taaaaatatt tttttcatag tagtcttatt ctatcataaa gaaaaaaaat attcttgctt
     9361 taataattca tctgttaagc tttttgaaaa tatggtaaaa taaagtgagg gaggtaactc
     9421 atgtttcgta gaaataaatt atttttttgg accacagaaa ttttactctt aaccatcatc
     9481 ttttacctat ggagacagat ggggtctttg attaaccctt ttgttagcgt gcttaataca
     9541 attatgattc catttttatt agggggcttt ctttattatt tgacaaaccc tattgttact
     9601 ttcttaaata aagtctgtaa actcaatcgt ttgcttggta ttttaattac cttgtgtact
     9661 ttggtctggg gaatggtcat aggtgttgtc tatctcttac ctattttgat taatcagtta
     9721 tctagtttga ttatatctag tcaaactatt tatagtcgag tacaagactt aatcatagac
     9781 ttatctaatt atcctgcgct ccagaatttg gatgtagaag ctacaattca gcagttaaac
     9841 ttatcctatg ttgatattct tcaaaatatc ctaaatagcg tatcaaatag tgtggggagc
     9901 gtcttgtcag ctcttatcag tactgttttg attttgatta tgactccagt ttttttggtt
     9961 tatttcttat tagatggaca taaattcttg cccatgcttg aaagaacgat tctaaagagg
    10021 gatcgcttgc atattgcagg cttattaaag aatttaaatg cgacgattgc tcgctatatt
    10081 agtggagttt cgattgacgc aatcattata ggttgtttag cttatattgg ctatagtatt
    10141 attggtttaa aatatgcttt agtttttgcc attttttctg gtgtagccaa tttaattcct
    10201 tatgtggggc caagtattgg tttgattcct atgatcatcg caaatatatt cactgatccc
    10261 catagactgc tgattgcagt gatttatatg cttgttgttc agcaggtaga tggcaatatc
    10321 ttatatcctc gaatcgtagg aagtgttatg aaggttcatc caatcacgat tttagtttta
    10381 cttttgttgt caagcaatat ttatggtgta gttggaatga ttgtcgcagt gccaacctat
    10441 tctatcttga aagaaatttc taagttctta tcccgtttgt atgaaaatca taaaataatg
    10501 aaagaacgag aaagagaatt agctaagtaa aagtcaggag aaccctgatt tttctttact
    10561 ggaagtggcc tttagattag aagactgaaa ataagttaaa gtcttaaact aattttcaca
    10621 gctaagaata gtagaagtta atctgataaa aatcgaaaaa accagtggaa ttccgtgtca
    10681 gggtaagttc cactggtttt catagtctat taaagttcga atgaaaccta tttatagtat
    10741 attgaaacta gaatagtaca cctctaattc taaaaca
//
LOCUS       DAVKJD010000060        10590 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKJD010000060 DAVKJD010000000
VERSION     DAVKJD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.060"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            307..720
                     /gene="rpsL"
                     /locus_tag="VW157_001554"
     CDS             307..720
                     /gene="rpsL"
                     /locus_tag="VW157_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9552832.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            740..1210
                     /gene="rpsG"
                     /locus_tag="VW157_001555"
     CDS             740..1210
                     /gene="rpsG"
                     /locus_tag="VW157_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9552833.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            1635..3716
                     /gene="fusA"
                     /locus_tag="VW157_001556"
     CDS             1635..3716
                     /gene="fusA"
                     /locus_tag="VW157_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9552834.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            3820..8211
                     /locus_tag="VW157_001557"
     CDS             3820..8211
                     /locus_tag="VW157_001557"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9552835.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VDKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGYPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNAAFDLGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            8313..8576
                     /locus_tag="VW157_001558"
     CDS             8313..8576
                     /locus_tag="VW157_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9552836.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            8569..8847
                     /locus_tag="VW157_001559"
     CDS             8569..8847
                     /locus_tag="VW157_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9552837.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            8868..9020
                     /locus_tag="VW157_001560"
     CDS             8868..9020
                     /locus_tag="VW157_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552838.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALREK"
     gene            9042..10283
                     /locus_tag="VW157_001561"
     CDS             9042..10283
                     /locus_tag="VW157_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9552839.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLYAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            10293..10523
                     /locus_tag="VW157_001562"
     CDS             10293..10523
                     /locus_tag="VW157_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9552840.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
BASE COUNT         3125 a         2040 c         2506 g         2919 t
ORIGIN      
        1 tactatttta tatatcagta taaatgacgg agataaaaat gtcacataag ccaagccgta
       61 ttattaggaa gatgatgtgt tcccagctat ttttattcag aaattgatct ctaacattac
      121 caataaaaaa gaaaaatatc ttgacaagca agggaaaatt ttgttacaat aatagacggt
      181 actttttact tttggtctct caagagtgta cagggacgtg ctgacaaatg ttgcaaaagt
      241 acacacagat gatatctgtc accaagtgta tcatcaccaa aaataaaaaa acacaggaga
      301 atgtagatgc ctacaattaa ccaattggtt cgcaaaccgc gtaaatcaaa agtagaaaaa
      361 tctaaatcac cagctttgaa cgttggttac aatagtcata aaaaagttca aacaaacgtt
      421 tcttcaccac aaaaacgtgg tgttgcaact cgtgttggaa caatgacacc taaaaaacct
      481 aactcagccc ttcgtaaatt cgctcgtgta cgtttgagca accttatcga agttactgcc
      541 tacatcccag gtatcggaca caacttgcaa gagcacagcg tggtgcttct tcgcggtgga
      601 cgtgtaaaag accttccagg ggtacgttac catatcgtcc gtggtgcact tgatactgca
      661 ggtgttaacg atcgtaaaca aggccgttct aaatacggta ctaaacgtcc aaaagcataa
      721 ggaaagggga taaagagaaa tgagtcgtaa aaatagagct ccaaaacgtg acgtattgcc
      781 agatccgcta tacaattcac aactagttac tcgtcttatc aaccgcgtta tgcttgatgg
      841 taaacgtggt actgctgctt caatcgttta cggtgctttt gagcaaatca aagaagctac
      901 tggcaacgat gcacttgaag tatttgaaac agctatggaa aacatcatgc ctgtacttga
      961 agtacgtgca cgtcgtgttg gtggttctaa ctaccaagtc ccagttgaag ttcgtccaga
     1021 acgtcgtaca acacttggac ttcgttggtt ggtaacaatc gctcgtcttc gtggtgaaca
     1081 cacaatgcaa gaccgtcttg caaaagaaat cttggatgct gctaacaaca ctggtgcagc
     1141 agttaagaaa cgtgaagata ctcaccgtat ggctgaagct aaccgtgcat tcgcacactt
     1201 ccgttggtaa gataggatgc gaaagcgtta agaaagtccc agagaaaata gggaatcgaa
     1261 gcaggttgcg gttgcaacca atgagattca tctttttctc cagactttta gcttgagctc
     1321 aactaaatca tgatgctagg aacggtaagg atgtaaggtg aaaataggaa actgacgcag
     1381 tattcgacga atacaaggag ttttatcttt ttcacgcggc atctcgttcc agctcacatc
     1441 ggctaactaa ctttagcccg ggttcaaatt agctaaatcg attagtatta gctataactc
     1501 agcttaccat ctcgtaagtt gaaaccaaca atagcatgaa aacattgaga acgggtaggt
     1561 cctgcctatc cgtttttatt aaaatcgtgt tataatagaa tagaaatcaa aaataaatag
     1621 gagaaacaaa cctcatggca cgcgaatttt cacttgaaaa aactcgtaat atcggtatca
     1681 tggctcacgt cgatgccggt aaaacaacaa ctactgagcg tattctttac tacactggta
     1741 aaatccacaa aatcggtgaa actcacgaag gtgcgtcaca aatggactgg atggagcaag
     1801 agcaagaacg tggtatcacg atcacatctg ctgcgacaac agctcaatgg aacaaccacc
     1861 gcgtaaacat catcgacaca ccaggacacg tggacttcac aatcgaagta caacgttctc
     1921 ttcgtgtatt ggatggtgcg gttaccgttc ttgactcaca atcaggtgtt gagcctcaaa
     1981 ctgaaacagt ttggcgtcaa gcgactgagt acggagttcc acgtatcgta tttgccaaca
     2041 aaatggacaa aatcggtgct gacttccttt actctgtaag cacacttcac gatcgtcttc
     2101 aagcaaatgc acacccaatc caattgccaa tcggttctga agatgacttc cgtggtatca
     2161 ttgacttgat caagatgaaa gctgaaatct atactaacga ccttggtacg gatatccttg
     2221 aagaagacat cccagctgaa taccttgacc aagctcaaga ataccgtgaa aaattgattg
     2281 aagcagttgc tgaaactgac gaagaattga tgatgaaata cctcgaaggt gaagaaatca
     2341 ctaacgaaga attgaaagct ggtatccgta aagcgactat caacgttgaa ttcttcccag
     2401 tattgtgtgg ttcagccttc aaaaacaaag gtgttcaatt gatgcttgat gcggttatcg
     2461 actaccttcc aagcccactt gacatcccag caatcaaagg tattaaccca gatacagacg
     2521 ctgaagaaat tcgtccagca tctgacgaag agccatttgc agctcttgcc ttcaagatca
     2581 tgactgaccc attcgtaggt cgtttgacat tcttccgtgt ttactcaggt gttcttcaat
     2641 caggttcata cgtattgaat acttctaaag gtaaacgtga acgtatcgga cgtatccttc
     2701 aaatgcacgc taacagccgt caagaaatcg acactgttta ctcaggtgat atcgctgctg
     2761 ccgttggttt gaaagatact acaactggtg actcattgac agatgaaaaa gctaaaatca
     2821 tccttgagtc aatcaacgtt ccagaaccag ttatccaatt gatggttgag ccaaaatcta
     2881 aagctgacca agacaagatg ggtatcgccc ttcaaaaatt ggctgaagaa gatccaacat
     2941 tccgcgttga aacaaacgtt gaaactggtg aaacagttat ctcaggtatg ggtgaacttc
     3001 accttgacgt ccttgttgat cgtatgcgtc gtgagttcaa agttgaagcg aacgtaggtg
     3061 ctcctcaagt atcttaccgt gaaacattcc gcgcttctac tcaagcacgt ggattcttca
     3121 aacgtcagtc tggtggtaaa ggtcaattcg gtgatgtgtg gattgaattt actccaaacg
     3181 aagaaggtaa aggattcgaa ttcgaaaacg caatcgtcgg tggtgtggtt cctcgtgaat
     3241 ttatcccagc ggttgaaaaa ggtttggtag aatctatggc taacggtgtt cttgcaggtt
     3301 acccaatggt tgacgttaaa gctaagcttt atgatggttc atatcacgat gtcgactcat
     3361 ctgaaactgc cttcaagatt gcggcttcac tttcccttaa agaagctgct aaatcagcac
     3421 aaccagctat ccttgaacca atgatgcttg taacaatcac tgttccagaa gaaaaccttg
     3481 gtgatgttat gggtcacgta actgctcgtc gtggacgtgt agatggtatg gaagcacacg
     3541 gtaacagcca aatcgttcgt gcttacgttc cacttgctga aatgttcggt tacgcaacag
     3601 ttcttcgttc tgcatctcaa ggacgtggta cattcatgat ggtatttgac cactacgaag
     3661 atgtacctaa gtcagtacaa gaagaaatta ttaagaaaaa taaaggtgaa gactaatccg
     3721 tcctcactct agaaggaagt cacttagtgg cttccttttg tctttagaaa atacctctaa
     3781 atatggtaaa atagtagaag aataatgtga ggaaaatgaa tgtcaaatag ttttgaaatt
     3841 ttgatgaatc aattggggat gcctgctgaa atgagacagg ctcctgcttt agcacaggcc
     3901 aatattgagc gagttgtggt tcataaaatt agtaaggtat gggagtttca tttcgtattt
     3961 tctaatattt taccgattga aatcttttta gaattaaaga aaggtttgag cgaagaattt
     4021 tctaagacag gcaataaagc tgtttttgaa attaaggctc ggtctcaaga attttcaaat
     4081 cagctcttgc agtcctacta tagggaggct ttctctgaag gtccatgtgc tagtcaaggt
     4141 tttaagtccc tttatcaaaa tttgcaagtt cgtgctgagg gcaatcagct atttattgaa
     4201 ggatctgaag cgattgataa ggaacatttt aagaagaatc atcttcctaa tttagccaaa
     4261 caacttgaaa agtttggttt tccaactttt aactgtcaag tcgataagaa tgatgtcctg
     4321 acccaagagc aggaagaggc ctttcatgct gaaaatgagc agattgttca agctgccaat
     4381 gaggaagcgc tccgtgctat ggaacaactg gagcagatgg cacctcctcc agcggaagag
     4441 aaaccagtct ttgattttca agcgaaaaaa gctgcagcta aacccaagct ggataaggcg
     4501 gagattactc ctatgatcga agtgacgaca gaggaaaatc gtctggtatt tgaaggggtt
     4561 gtttttgatg tggagcaaaa agtgactaga acaggtcgtg ttttaatcaa ctttaaaatg
     4621 acggactaca cttcaagttt ttctatgcaa aagtgggtta aaaacgagga agaggcccag
     4681 aagtttgatc tcatcaagaa gaattcttgg ctccgagttc gagggaatgt ggagatgaat
     4741 aacttcacac gtgatttgac tatgaacgta caggatctgc aggaagttgt tcactatgag
     4801 cggaaggatt tgatgccaga aggtgagcgt cgggttgagt ttcatgctca tactaacatg
     4861 tcgactatgg atgctttgcc agaggtcgaa gagattgttg caacagctgc taaatgggga
     4921 cacaaggcgg ttgctatcac ggaccatggg aatgtccagt cctttccaca tggctataag
     4981 gcggctaaga aagcgggaat ccagctgatc tatgggatgg aagccaatat cgtggaggac
     5041 cgtgtcccta tcgtctataa cgaagtggag atggacttgt cagaagcaac ctacgtggtc
     5101 tttgacgtgg aaacgacggg actttcagct atctataatg acttgattca ggttgcggcc
     5161 tctaagatgt acaaggggaa tgttattgct gaatttgatg aatttatcaa tcctgggtat
     5221 cccttgtcag cctttactac agagttaact ggaattacag atgatcatgt caaaaatgcc
     5281 aaaccactag aacaagtttt gcaagaattc caagaatttt gcaaggatac ggtcctagtt
     5341 gcccacaatg ctgcctttga ccttggcttt atgaatgcta attatgagcg tcatggtctt
     5401 ccaaagatta gtcagccagt tattgatacg ctggagtttg ctagaaacct ctatcctgag
     5461 tataaacgcc atggtttggg gcctttgacc aagcgttttg gtgtggcctt ggaacatcac
     5521 cacatggcca attacgatgc ggaagcgact ggtcgtctgc ttttcatctt tatcaaagag
     5581 gtagcagaaa aacatggtgt gaccgattta gctagactca acattgatct aatcagtcca
     5641 gattcttaca aaaaagctcg gatcaagcat gcgaccatct atgtcaagaa tcaggtaggt
     5701 ctaaaaaata tctttaagct ggtttccttg tctaatacca agtattttga aggagtgcca
     5761 cggattccga gaacggttct agatgcccat cgagagggct tgattttagg ttcagcctgt
     5821 tcagagggtg aagtttttga cgtggtcgtt tctcaaggtg tggatgcggc ggttgaggtg
     5881 gccaagtatt atgactttat cgaggtcatg ccaccggcta tctatgcgcc cttgattgct
     5941 aaagagcagg tcaaggatat ggaggaactc cagaccatta tcaagagttt gatagaggtt
     6001 ggagaccgcc ttggcaagcc tgttttggct acgggaaatg ttcactatat cgaaccggaa
     6061 gaagagattt atcgtgaaat tatcgtccgt agtttgggac agggtgcgat gattaaccga
     6121 actatcggtc atggtgaaca tgcccaacca gcgccacttc caaaggctca ttttcgaacg
     6181 actaatgaga tgttggatga atttgccttt ttgggagagg aactggctcg taaactggtt
     6241 attgaaaaca ccaatgcctt ggcagaaata tttgaacccg ttgaagtcgt taagggtgac
     6301 ttgtatacgc ctttcatcga caaggctgaa gaaacagttg ctgagttgac ctataagaaa
     6361 gcttttgaga tttatggaaa tccgctgcca gatattgttg atttgcggat tgaaaaagaa
     6421 ttaacatcca tactggggaa tggatttgct gtgatttatt tggcatcgca gatgctggtg
     6481 caacgttcta atgaacgggg ttatttggtt ggttctcgtg ggtctgtcgg atctagtttc
     6541 gttgcgacca tgattgggat tacggaggtc aatcctctct ctcctcacta tgtctgtggt
     6601 cagtgtcagt acagtgagtt tatcacagat ggttcgtacg gttcaggatt tgatatgccc
     6661 cataaggact gtccaaactg tggtcacaaa ctcagtaaaa acggacagga tattccgttt
     6721 gagaccttcc ttggttttga tggggataag gttcctgata ttgacttgaa cttctcggga
     6781 gaagatcagc ctagcgccca cttggatgtg cgtgatatct ttggtgaaga atatgccttc
     6841 cgtgcgggaa cggttggtac agtagctgcc aagactgcct atggatttgt caagggttac
     6901 gagcgagatt atggcaagtt ttatcgtgat gcagaagtag aacgcctcgc tcaaggagcg
     6961 gcgggtgtca agcggacaac aggacaacac ccggggggaa tcgttgttat tccgaactac
     7021 atggatgtct acgattttac gcctgtccag tatccagcag atgatgtcac ggctgaatgg
     7081 cagaccactc actttaactt ccacgatatc gatgagaacg tcctcaaact cgatgtactg
     7141 ggacatgatg atccgaccat gattcgaaaa cttcaggatt tgtctggtat tgaccctaat
     7201 aaaattccta tggatgacga aggcgtgatg gcactctttt ctgggactga tgtgctaggg
     7261 gtaacacctg aacaaattgg aacgcctacg ggtatgttgg ggattccaga gtttggaaca
     7321 aatttcgtac gtggaatggt agacgaaacc catccgacaa cctttgcgga attgcttcag
     7381 ctgtctggtc tgtcccacgg tactgacgtt tggttgggga atgctcagga tctgattaag
     7441 caaggaatag cggacctatc gactgttatc ggttgtcggg acgacatcat ggtttacctc
     7501 atgcatgcgg gtctggaacc taagatggcc tttaccatta tggaacgggt acgtaagggt
     7561 ttgtggctaa agatttcaga agaggagaga aatgactata tcgaagccat gaaggctaat
     7621 aaggtgccag agtggtatat cgaatcctgt gggaaaatta agtacatgtt ccctaaggcc
     7681 catgcggcag cctacgttat gatggccttg cgtgtagctt acttcaaggt tcaccatcct
     7741 atttattact actgtgctta cttctccatt cgtgctaagg cttttgatat caagaccatg
     7801 ggtgcgggct tggaggtcat caagcgcaga atggaagaaa tctctgaaaa acggaagaac
     7861 aatgaagcct ctaatgtgga aatcgatctc tatacaactc ttgagattgt caatgagatg
     7921 tgggaacgag gtttcaagtt tggtaaatta gatctctact gtagtcaggc gacagagttc
     7981 ctcatcgacg gggataccct tatcccacca tttgtagcaa tggatggtct gggagagaac
     8041 gttgccaagc aactggtgcg ggcgcgtgaa gagggagaat tcctctctaa aacagaacta
     8101 cgcaagcgtg gtggactctc atcaaccttg gttgaaaaga tggatgagat gggtattctt
     8161 ggaaatatgc cagaggataa ccagttgagt ttgtttgatg agttgtttta aaaaattgct
     8221 taataatcta ttaaaagagg ctaacgtata tccaatagat ttacattagc tttctttttt
     8281 gttaaaatag tctatggaaa gagggtgaga gtatgtcaaa gatgagtata agcatccgtc
     8341 tggatagtga ggttaaggag caggcccaac aggtgtttag taatctggga atggatatga
     8401 caacagctat taatattttc cttcgtcagg caattcaata tcagggatta ccttttgatg
     8461 ttagactaga cgaaaatcgg aagttgctcc aagcgttaac ggatttagac caaaatcgta
     8521 atatgagcca gtcttttgaa tcagtctcag atttgatgga ggacttacgt gcttaagatt
     8581 cgttatcata aacagtttaa aaaagatttt aagttggcta tgaagcgtgg tttgaaggca
     8641 gaattattag aagaagtttt gaattttctg gttcaagaaa aagaacatcc tgccagatat
     8701 cgtgatcatt cattgacggc atccaagcat tttcaaggag ttcgtgaatg ccatacccag
     8761 ccagattggc ttttggttta taaagtagac aagtcggaat tgattttaaa tttgctgagg
     8821 acaggcagtc acagtgattt attttaatct attttaaggg ggttctcatg aaactaagaa
     8881 tatttgcgga agataagccg gctaagaagg tatttgaata tcaattagaa cttgctgatc
     8941 gtacaattct tctatcgaca gcactcttgt caggtgctat tgctttagca ggaatctttt
     9001 ctgctttgag agaaaaataa aaatagaaaa gagaaaacag aatggtttta ccaaatttta
     9061 aagaaaatct agaaaaatat gcgaaattgt tggttgcgaa cggaattaac gtgcaacctg
     9121 gtcacacttt ggctctctct attgatgtgg agcaacgtga attggcacat ctaatcgtga
     9181 aagaagctta tgctctgggt gcgcatgagg ttatcgttca gtggacagat gatgtcatta
     9241 accgtgagaa attcctctat gcgccaatgg agcgtttgga caatgtgcca gaatacaaga
     9301 ttgctgagat gaactatctt ttggagaaca aggctagccg tcttggggtt cgttcatctg
     9361 atccaggtgc cttgaacgga gtggatgctg acaagctttc agcttctgct aaagctatgg
     9421 gacttgccat gaagcctatg cgtatcgcaa ctcaatctaa caaggttagc tggactgtag
     9481 cagctgcagc aggacttgag tgggctaaga aagtcttccc aaatgctgcg agcgacgaag
     9541 aagcagttga tttcctttgg gaccaaattt tcaaaacttg ccgtgtctac gaagcagatc
     9601 ctgttaaggc ctgggaagag catgcagcta tcttgaagag caaggccgat atgcttaata
     9661 aggagcaatt ttcagccctt cactacacag cgccaggaac agatttaaca cttggtttgc
     9721 caaagaacca cgtttgggaa tcagctggtg ctgtcaatgc acagggcgaa ggattcttgc
     9781 caaatatgcc gacagaagag gtcttcacag cgcctgactt ccgtcgtgca gatggttatg
     9841 tcacttctac aaaaccgctt agctacaacg gaaatatcat cgaaggtatt aaggtgacct
     9901 ttaaggatgg acaaatcgta gatatcactg ctgagaaggg tgatcaggtt atgaaagacc
     9961 ttgtctttga aaatgcgggt gcgcgtgcct tgggtgaatg tgccttggta ccagatccaa
    10021 gtccaatttc tcagtcaggc attaccttct ttaacaccct tttcgatgaa aatgcgtcaa
    10081 accacttggc tatcggtgca gcctatgcga ctagcgttgt tgatggagcg gagatgagcg
    10141 aagaggagct tgaagctgca gggcttaacc gttcagatgt tcacgtagac tttatgattg
    10201 gttctaacca aatggatatc gatggtattc gtgaggatgg aacgcgagta cctcttttcc
    10261 gtaatgggaa ttgggcaaat taaggagata atatgttagg aagtatgttc gttggtctcc
    10321 tagtgggatt tttagcaggt gctatgacca atcgtggaga gcgaatggga tgttttggaa
    10381 aaatgtttct cggttggatc ggagcctttc taggtcactt gctctttgga acttgggggc
    10441 cagttttatc aggaacagct attatcccag cgattttagg agctatgatt gttttagcta
    10501 ttttttggag acgaggaagt taatttccta aatctgatac tcaatgaaaa tcaaagagca
    10561 aactaggaaa ctagccgcag gttgctcaaa
//
LOCUS       DAVKJD010000061        10424 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKJD010000061 DAVKJD010000000
VERSION     DAVKJD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.061"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..466)
                     /locus_tag="VW157_001563"
     CDS             complement(<1..466)
                     /locus_tag="VW157_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552841.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCHQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIG"
     gene            complement(466..1230)
                     /locus_tag="VW157_001564"
     CDS             complement(466..1230)
                     /locus_tag="VW157_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552842.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMMVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(1326..1729)
                     /locus_tag="VW157_001565"
                     /pseudo
     CDS             complement(1326..1729)
                     /locus_tag="VW157_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1909..2541)
                     /locus_tag="VW157_001566"
     CDS             complement(1909..2541)
                     /locus_tag="VW157_001566"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9552843.1"
                     /translation="MILAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVDAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(2575..3276)
                     /gene="pyrF"
                     /locus_tag="VW157_001567"
     CDS             complement(2575..3276)
                     /gene="pyrF"
                     /locus_tag="VW157_001567"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9552844.1"
                     /translation="MREHRPVIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            complement(3509..4720)
                     /gene="divIB"
                     /locus_tag="VW157_001568"
     CDS             complement(3509..4720)
                     /gene="divIB"
                     /locus_tag="VW157_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9552845.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVVSSEADKEKEEPESKEK
                     EEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKDIRV
                     EGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTKFTI
                     KVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFVSEL
                     AQISPELKVAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKPQLS
                     EPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKRLEEEQKKQEEESN
                     RNQTNQRSSRR"
     gene            complement(4730..5788)
                     /locus_tag="VW157_001569"
     CDS             complement(4730..5788)
                     /locus_tag="VW157_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9552846.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(5792..7144)
                     /locus_tag="VW157_001570"
     CDS             complement(5792..7144)
                     /locus_tag="VW157_001570"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9552847.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKRIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(7248..7889)
                     /locus_tag="VW157_001571"
     CDS             complement(7248..7889)
                     /locus_tag="VW157_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552848.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(7891..9911)
                     /locus_tag="VW157_001572"
                     /pseudo
     CDS             complement(7891..9911)
                     /locus_tag="VW157_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(9982..10281)
                     /locus_tag="VW157_001573"
                     /pseudo
     CDS             complement(9982..10281)
                     /locus_tag="VW157_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
BASE COUNT         3181 a         2277 c         1830 g         3136 t
ORIGIN      
        1 atcctataaa gaaactagca ttgtttgcaa tacttatacc ataaaattct cttaaaaaat
       61 caacctcctt tatctccaaa gagaagctaa aaccattact aaatgcaatt agaaaaatca
      121 ataaaaataa agtcgccgtc caaatccccg tactaagagc tgctaatttg aaactaaaac
      181 tggtaaagtg cttaattgat ttcagacgaa tgcgacactc caacctatta aaatagttat
      241 tcatcaaata aaaaaagaac aatatatatg tgaacggaaa gcaatatact ccagtcgtca
      301 tatcttgaag taaaactaag atccattcta atacatttgg atggattgaa tattggtgac
      361 agcgcaataa atatactgta ctagataaaa cacaggatag cagtaatata aaataaacca
      421 atactgctaa gaaatctttt ttataaaatt gaacaaattg tttcattata catagtcctc
      481 tgaatgtaga aaaaatgtac cataaacaac caaacaacta acaaataaaa taaaagcaag
      541 atgcccacta actaaggaaa gactgatatc tttctgatat cccaaagcta gcgttgtcac
      601 aggttctaag taagatagcc ctaaaatagc ccataaaata ccaccaacca tcatataggc
      661 aactgggatg aaaatagctc ctaatttttt cttcactagc aaagcactag ctagtccaaa
      721 aatagagaac acagcgcccc aaactccata ccagagagtc gtcacaagac tatagagcaa
      781 ctgattagaa tcaaataatt cttttaaggc accactataa tctccaatat aaatttcctg
      841 ataaggagtc actaaaagat taattcctaa taataataaa taggggagaa aaaagactag
      901 aaaagaagaa ataattacag tactacccac aatagccaga tacttctttt tagaaattcg
      961 acacaattgt gctgttagaa aatgactctc agcatcctct attatctgac tagaataggg
     1021 cagtgtacag ataagtgcag ctactaggct aatcggtgaa aatcctcgaa tagaagaagc
     1081 ggcaaaaaaa atcgataaac cttcaatttt ataaatacca ttgaaagcaa ggaaatttcc
     1141 taaactcatg caaagaaggg tcaaaaataa agacatataa aatcgaggtg attgaacgac
     1201 tcgtacaaga ttacaaatga aaaattccat ccttactcct ccttataata aaaatagggt
     1261 gtagcattct tttttcatgc tacacccaca atcaaccatc tttaaggctt actctgacaa
     1321 gtaagttaat aagaatctga actccaagaa cctgaagtat gaattcttac atgatttcca
     1381 aattgtggcg ccatagctaa tctagtacca gaaccaatat aattgtcacc acctccatta
     1441 tagtacatga caatcctaga gccagacccc aatgaatata ccggggtaat atctgaccca
     1501 ctataggcgc tacgaataga ggtatttaac cttttaccgc caccagtgct gtcactatta
     1561 ttaattccag cagaggcgtt ttctttctca agctcactag ttgtatccga accccaacgc
     1621 ggcaacgtta aatcataatt tccaatctgt ctagctaaaa ctgtagggag aaacaaacta
     1681 gatgcaattg taactacgca aaaaaggaat atatttttct tcttttcatg acatcgtcct
     1741 cttaagaagt gataaacaag atagttgatt gatcaatttg atacatatta cattttcatt
     1801 atatcaataa aagaaagcgt tgtcaattat tttatttaaa atattgtcat attttaaata
     1861 aaatggtaaa agagactcgg tagcctcgtc tctctttatc ttactgacct aaccttcttg
     1921 ccaattttct tggtcttctt taaagcgttt tagaagatca aggccctctg gcgtgatgta
     1981 accttcttct tgggctagat ggataagctc gctatagttt gaaagcgtca caagtttaac
     2041 accagcatct gcaaagttct tatctgcttt tggcaattgg tagctgaaaa tcgctacaac
     2101 tccaagcaca tctgctcctt ctcgcttggc tgctgctaca gcttcaagaa ctgaaccacc
     2161 cgttgaaata aggtcttcaa ccactaccat tttttgacct tgagctacgc gaccttcgat
     2221 ttgattacca gctccatggt cttttggttt gctacggatg taggcaaaag gcaagtccat
     2281 cttatcagca ataatggctc cgtgtggaat ccctgctgtt gcagttcctg caatcacttc
     2341 tacttcagga aaggcttctt tgatagcatc cacaaaacca ttttcaatta gggtacgagt
     2401 ttctggatag gctagtgtca cacgattatc agtgtaaatc ggtgacttga taccagatgc
     2461 ccaagtgaag ggttcctctg gtttgaggta aacggcttgg attttcaaga ggtggctagc
     2521 gatatcttta gcaagtatca tggtattctc cttttatttt tctaatctag ttctttaatt
     2581 ccagtcctgt gtccattcat ccttgatggc atgataagct gcaacaggat cctcagcttg
     2641 ggtaatggga cgtcccacta cgatatagtc actgccgatt tgataggcat cagcaggtgt
     2701 catgactcgt ttttgatctc caactgcagc accagctgga cgaatccctg gtgtcagaca
     2761 gataaaatct ggattggtag cctgcttgat gacttgtact tcctgagccg agcaaacaac
     2821 accatccaag ccagcttcag ctgtcttctt ggcatagtga atcacagact cttgcagact
     2881 ggtttggata ttttgaaact cctgcatctg ggcttctgac gttgatgtga gctgcgttac
     2941 agcgatcaat ttggcttgac ttccaagacc ttcacgcgca gccttcatca tctctacacc
     3001 accagccgca tgaacattgg tcatatccac accaagctga gacaagacct tcatggctga
     3061 cttgactgta ttaggaatgt catgaagttt gagatccaaa aagacactat gacccaaacc
     3121 ttttaagtag gacacaatct caggccccgc tgcgtaataa agctccatcc ctaccttgag
     3181 ataaaggctt tcttctgctg ggaaaagagc taaaaattcc ttgaccgcct caaaactagg
     3241 aaaatcaaga gcaatgactg gacgatgttc tcgcataggt ttctcctttt acctttacta
     3301 gtgagagagt ctgaccaaca gaagccacga aaaaaggaac ctgccaacag caaggttcca
     3361 cgaaaaatgg gtagtctcca ccctttcacc gtctaacctt agctgcctct ctggactgct
     3421 ttaaaggttt tttactattc tattatttct actagcactt gtcaagtaaa aacatggtca
     3481 ctaaagaact ataagagaaa aggtaaacct agcgacgcga tgaacgctga tttgtttgat
     3541 ttcgattgct ctcttcctct tgttttttct gttcttcttc taaacgtttc tgttcttctt
     3601 cctgtttttt ctcggcttcc ttggcctctt gtttggcttt ttcctcagcc tccataatta
     3661 atttatccgc cacagtgtag ctgtagattc cagcttccat gtcgaccaca ctcggttctg
     3721 acaattgtgg cttaatctta ctgtaatatg gcagtttctt actcatttca gatagaggaa
     3781 ccaagacttc gtccgaatca ttcatggtca atcgaattaa atcggatgtc accttgcttg
     3841 gggctaattc caccttttgg atagccacct tgagttctgg gctaatttga gcaagttctg
     3901 agacaaaaac cttgatttgt tcactatcat taaagagaac tgataaataa gtttctggta
     3961 aactgttcag actcacagaa ctagtctcaa gctgaccact ggaaagaata ggataatgat
     4021 tttcaccaga aatatagtag gccacaatat catattcctt gaccttaata gtgaacttag
     4081 ttggaaattg atagacaagt tgagctgatt caacccaata gttagactta atctgctttt
     4141 catattttgc cttgtctagc agaaggttaa tcgtataatc cgaatcctga atgcctgaag
     4201 cctgtcgaat atcatcagct gtagtttgca ccgttccctc aacacgaata tctttcatgg
     4261 tcgcataagg actgagcaag taggcagaga caatcaataa aagcagactt ggaaataaaa
     4321 tcgtgaaggc tcgcaagata tggataccag gaatctttgc tttggctggt ttttcctttg
     4381 tagccttttt agaaagcttt ttatcctgtt cctccttctc tttagactct ggttcttctt
     4441 tctctttgtc agcctctgag gatactactt tttcttcaga ctcttcctta gctgattctg
     4501 aatcttcctg gtctgtttca ctctcctggt cctgtttatc ctctgacttc tcagattctt
     4561 ctcccattcg agcttgtctt tccttttcct tctcctcagc tagagccgcc tcttcttcag
     4621 ccttcttttt tagatattct tggtttcgtt tctgccattc tgataactct ttcaattctt
     4681 cgagggtttc tttgtcctca tttttcttat cttttgacat ttactttcct tatgataaat
     4741 cttttttcaa caattgataa aaatctgcta gagatttcaa ttccttagaa gctttcatct
     4801 tagcttggta atcttccttg tgacttagta agtgagaaag cttctcttcc aaactatcca
     4861 aggtcaaatc actttcttga aggtcttctg catagccttt ttttacaaag taagctgcat
     4921 tttcaatctg gtcaccacga ctagcttcac gaccaagcgg cacaatgaca tgcaattttg
     4981 ctatcgccaa gagctcaaaa atcgtattgg caccacctcg tgtcacaaca atatcagcca
     5041 attccatcaa gggttgatag agatcggtca catagtcaac acgaaaaaga ttttgcctca
     5101 actcattcag actagaatct ccagttagat tgataatatt gtagcgctct gttagttctt
     5161 tcttatggtc tgtcaccaat tggttaaaga cacgagcacc tgcagaaccg ccgacaaaca
     5221 atacagttgg caatttggga ttaaagtggg tttgaatatc caccaattca tctggttctg
     5281 gagtgttttt gtccgaaacc ttggtcaccg ctcccacatg ctcgacctta gccaaactcg
     5341 aagcttgttc aaaggttgaa tacatcttag tcgcaaattt ataggcgatt ttattggcca
     5401 agcccataga caggtcagat tcgtgaataa agacaggcac tcctgacaca cgcgcagcga
     5461 taacaggcgg tactgagaca aagcccccct ttgaaaaaag ggtctgtgga cgcagtcgca
     5521 acatgataaa gagcgattgg acgattcccc agccaacttt gaagacgtcc agcatatttt
     5581 gccaagagaa atagcgacgc aatttcccag tcgcaatgga gtggaaagtg acatccaaac
     5641 ctgacttaag gatttcttgg tgttcgatac cacgcttgtc cccgatatag tggacttccc
     5701 aaccatcttc gatgaacttg ggcattaaca aaagattgag ggtaacgtgt ccaaccgtcc
     5761 ccccacctgt aaagacaatt tttttcatat tttattcttt taactccgct actgtgtcga
     5821 taaagaggtc gccacgtact tcaaagttag catacatatc ccagctggca ttggcaggac
     5881 taagaagaac cacatctcct tgagtcgcaa gttcataggc cttgcgggtc gcatctgcaa
     5941 tatctgtcgc ctccacataa gcgacaccag ccttgtctgc tgcccgtttg acacgttctg
     6001 cagattgacc caggatgacc atcttcttga gtccagtaat gtctggcacc aattcgtcaa
     6061 actcattgcc acggtccaaa ccacctgcaa tcaagacgac cttgctgttg tcaaatcctg
     6121 acaaggcttt ttgagtagcc aagatattag ttgatttact atcgttatag aatttaacac
     6181 ccttgatgtc atccacaaac tggagacggt gtttgacacc accgaaggct gaaagagttt
     6241 ccttgatggt ttgattgtcc acaccacgaa gcttggctac agcaatagtc gcaagggcat
     6301 tttccacatt gtggctacct ggaacaccga tttcattcgc tgccatgact acttcaccac
     6361 ggaagtagag ttgaccatct tccagataag ctccatcaac cttttcaagt gttgaaaatg
     6421 gtacaacagt ggcttctgtc ttggaagcca agtcttttgc caagtcttga ttaaagttca
     6481 agacaaggaa atcagctgct gtcatcttgt tctggatatt ccacttggct gctacatatt
     6541 cagaaaatga cccatggtag tcgatatgag ttggcatgag gttggtaata accgcaatct
     6601 ctggatggaa ttcttgaaca cccatgagtt ggaaagaaga aagttccata acaagcgtgt
     6661 ccttatctga tgctatttga gcaacctgac tggcaggata gccgatattc cctgataaaa
     6721 gaccatgttg cccagcagca gtcaaaactt ccccaatcat agtcgttgtg gttgtcttac
     6781 cgttcgatcc tgtgatacca ataatcggtg cttctgaaat caaataagcc aattccacct
     6841 cagtcaagac tggaattctc ttggccaaag ccttttcaat catgggattg ctgtagggga
     6901 tacctggatt ttttaccatg agggcgaact cttcatccaa gagttccaaa ggatggccac
     6961 ctgtaatgac cttgatccct tcttccagca aacattgcgc agctggattg tcctcgaatg
     7021 gtttcccatc atttactgtc acaatggcac ctaacttgtc caacaaacga gccgcagatt
     7081 caccagactt ggccaaacct aaaacaagaa ctttcttatt tttaaattga tctattactt
     7141 tcatgtctcg aactccattt ctactcctac tattttacca tttttatgga aataaaaaag
     7201 ccacaaagtg tgtttgtgac tctttcttct aactgaatct taccatatca tctatgtgat
     7261 aaatcggtaa ctcgaatgac ctgatccact tgctcccaaa tcagaggatt atgggtcgca
     7321 ataataatgg tccgattcgg attttttaaa gattctagga tggaaagtaa ttcctcagag
     7381 tttttggggt ctagtgaagc ggttggttca tctgcgagga tcaaaggtgg atcctttaaa
     7441 attatcttcg ctagtgcaac acgttgtgct tctcctcctg ataactcaaa tataggttgc
     7501 ttcaaatcca aataagagag gtttacacgg tttagagctt gtttcatcaa agagattttc
     7561 tctttttcct tcaacttttt accaactaaa cccagattga gattctcttt gacggtttgg
     7621 ctttcaatta agccaaaatc ttgaaataag tatcctaagt aatctctaaa gaaaacagaa
     7681 ggcttgatgt ccttaagaga agtgccatca tagatgattt gccctttgtc atatggctcc
     7741 aatcgtccaa tcatattcaa gagtgttgtc ttaccacagc cacttgtacc gattaaggca
     7801 taaattttcc caccttcaaa atgaagattc gtatctgaaa atagctgacg gcttccaaat
     7861 tttttagata tattctttag ttcaatcatc ctattttcct ttcataattg tcataaaaac
     7921 acgagattct ttctgcgctt gacggtaaag cgtcaaaact gcactagcta gaaagaccaa
     7981 taaagtgagc aagccaatca ccaagtctcg actgcttaaa ataaagagac tagcaccaaa
     8041 tacaaaactg gcaaattggc taaccatata ctgagcatgt gtttcaaaaa atcgtaaacc
     8101 tgaaattcgt ttaatcaaga tatctcggcg gaattgctcg aaatatagaa gattgacaga
     8161 ataaaatagt aacaaggaac tagctatccc cacaatagct cctaagatta aagttgctgt
     8221 ttcagtttga acttcattat aacgagttag ataaacactt cttccttatt taagatagga
     8281 tacttgctca taaattccag ctttcttcaa gagttctagc ccactctcat atcctttgat
     8341 aaagagttgt tttccagcat tgatagacca actagataag gatataaaac tatcacctgt
     8401 agaagtcggc gtgaatacca ctaaaatcgg atcagtcaaa tactgagtgg atacgggatt
     8461 ctcaccgtta ttataaacaa accgcttttc tcccattgaa agataactaa cgtgcgcttt
     8521 catctcataa tccaaaggag cacttgcctc ctcaccagat tttccataat aactcaatct
     8581 ttcttcaaaa actttcttaa gttctgcttc tcgagagcgc aaatgttctg ggagcaagag
     8641 gataaactca cctttttgga gatgggctaa cttctgtttg gtctcagcat ctaccacgac
     8701 cttttccttg tccaaataac tgggactaac atagagcgta ttagcatctg gactataggt
     8761 atccagtgtc tctccctgtt cattttttcc ttgtggattg gcaaaatgga gcagattatc
     8821 ctttacataa agagcttgtt cttcttcgat tgcttccttg gcaaaggcat accacttgct
     8881 ctgattttct gtatcttttc ctctatcacc taagccaaaa gaaatttgat aatagtctgc
     8941 tctatcctgc catgcttgtt ttgaaatttc aagttctttc aatcgttggt aagacgtcaa
     9001 acctgtctta acagcgtatc ctactgtaaa gacagcaacc aactgacaca atagggttaa
     9061 aatcatcagg cgtttaaggg gtaatctccc cttaataacg ggaactaatg ctttgtaatt
     9121 caaactcatt agataaagga gcattagtaa aattgaaatc gccaataaaa acaacagata
     9181 gaaactaatc ccaaaaccat aggtggctaa caagatagga taaaacaaac cttgactaaa
     9241 aagaacgact cccccaccta ggaaggaaag gagggctgat agaaggagcc attgatatca
     9301 gtagataaag aatgccccat gatggataag agagtttgac cagaaaagag ttttatacct
     9361 gcagctctca tttccttaat ccgagtgata atcactaaag caaagaaaga taagccaaat
     9421 attgctaaac taattaaaat aaggggattt agtaatattc gaaaagcaag agaatagggc
     9481 ggtatctttc ggtcagcaat tgctttataa cccaaatctc ctaatttatc ggcaagcttt
     9541 tctttcgtca aggagcctga caaaaggaga taactattta gcggatcact acgttcacga
     9601 ctttcttggc tagcttcttg gaattctttt ggtaaagttc cctgaccata agttgcataa
     9661 gtaaagtgag tcgtcccatc cttactcggc tctacaattc ttctagctat taaactctgt
     9721 tctgaatttg caaaattctc caattcctgt tcaaatacct cacgcgtcgg tttctgagta
     9781 tcttttttga cacgaagtaa agaaacggaa tcatagcttg catataaata ttgtggcgca
     9841 cgtaagacaa taatccaagc aaggaagaag ctgagaaaaa aagttgataa taatatgaat
     9901 agtttcttca tagtagactc cttgtaaaca aaattccccc tgtaatttct tacaagggga
     9961 acgatttaaa tcaatgaacg attagtcata atcacagtaa aatgctactt gttctcccca
    10021 tttagtccaa atccatgcat aggaggtttt tccagcagga gcttctcctt tatcagaatg
    10081 accattccat ctgctcacga tggatgaata atgatatttt ttaccatgat agtaatttga
    10141 aaaagcctaa ccacctcctg aaccttctcc atatgtccat actcctccat ctggatatta
    10201 tacagcagct gatgcagctc ccaataatgt aaaacttgaa ataagagcta gagcaagtaa
    10261 tctatgtttt ttcgttttca ttttattttt tctttcaaaa aaatcacacc ttgagcaaca
    10321 atgcaacaaa ataaatcctc ctctctcttt tattgaaacc gctttcttgt gtgataagaa
    10381 taacattttt attatttgtt gtcaaggaaa aaatcgaatt tttt
//
LOCUS       DAVKJD010000062        10139 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKJD010000062 DAVKJD010000000
VERSION     DAVKJD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.062"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..179
                     /locus_tag="VW157_001574"
     CDS             <1..179
                     /locus_tag="VW157_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9552849.1"
                     /translation="KLELLCEEFGHKLLPLPPYSPEYNPIEKTWVHIKKNPKKVLPSC
                     NTFYEALLSCSCFN"
     gene            145..525
                     /locus_tag="VW157_001575"
     CDS             145..525
                     /locus_tag="VW157_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9552850.1"
                     /translation="MRLFCLVLVSIDYINCLSETTDKNWHKSDRVFVTNTGKTVHSSI
                     LSKSLQRANERLKKPIPKHLSPHIFRHTTISILSENKIPLKTITDRVDHSDSEVTTSI
                     YTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(613..1917)
                     /gene="eno"
                     /locus_tag="VW157_001576"
     CDS             complement(613..1917)
                     /gene="eno"
                     /locus_tag="VW157_001576"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9552851.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2081..2527
                     /locus_tag="VW157_001577"
     CDS             2081..2527
                     /locus_tag="VW157_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9552852.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2524..3639)
                     /locus_tag="VW157_001578"
     CDS             complement(2524..3639)
                     /locus_tag="VW157_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU9552853.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3648..4296)
                     /gene="serB"
                     /locus_tag="VW157_001579"
                     /pseudo
     CDS             complement(3648..4296)
                     /gene="serB"
                     /locus_tag="VW157_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
     gene            complement(4355..5788)
                     /gene="glgA"
                     /locus_tag="VW157_001580"
     CDS             complement(4355..5788)
                     /gene="glgA"
                     /locus_tag="VW157_001580"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9552854.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5785..6924)
                     /gene="glgD"
                     /locus_tag="VW157_001581"
     CDS             complement(5785..6924)
                     /gene="glgD"
                     /locus_tag="VW157_001581"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9552855.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(6914..8056)
                     /locus_tag="VW157_001582"
     CDS             complement(6914..8056)
                     /locus_tag="VW157_001582"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9552856.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     IRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTIKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8046..9974)
                     /gene="glgB"
                     /locus_tag="VW157_001583"
     CDS             complement(8046..9974)
                     /gene="glgB"
                     /locus_tag="VW157_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9552857.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3074 a         2248 c         1788 g         3029 t
ORIGIN      
        1 ggaagctaga actcttgtgt gaagagtttg ggcataaact tttacctctt cctccctact
       61 cacctgagta caatcctatt gagaaaacat gggttcatat caaaaagaac cccaaaaagg
      121 tattaccaag ttgcaatacc ttttatgagg ctcttttgtc ttgttcttgt ttcaattgac
      181 tatataaatt gtctaagcga aacaaccgat aagaattggc acaaaagtga ccgtgttttt
      241 gttaccaata caggaaaaac agttcatagt tctatcttga gcaagtctct ccagcgagca
      301 aacgaacgcc ttaaaaaacc aattcccaaa catctgtccc ctcacatctt cagacacacc
      361 actattagca tcttatcaga aaataaaatt cctttaaaaa caatcacgga cagggttgat
      421 cattccgact ctgaagtcac tacttccatc tacacccacg tcacaaagaa catgaaagat
      481 gaagcaatca atgtactgga taaagttatg aaaaagattt tttaaaaagt tttgtccctt
      541 ttttgccctc taaatacaaa aatagccctt cggataaaat ccgaggggct agaaacgttg
      601 ttaaatcaac gattattttt taaggttgta gaatgatttc aatccacgat attcagctac
      661 ttcaccaagt tggtcttcga tacgaagcaa ttggttgtat ttagcgatac ggtctgtacg
      721 tgaaagtgaa ccagtcttga tttgtcctgc gttagttgca actgcgatat cagcgattgt
      781 tgaatcttca gtttcacctg aacggtgtga tacaacagca gtgtaaccag cttctttagc
      841 catttcgata gcttcaaaag tttcagtaag agtaccgatt tggttaactt tgataaggat
      901 tgagttagca gcaccttctt ggataccacg tgcaaggtag tcagtgtttg ttacgaagaa
      961 gtcgtcacca acaagttgta ctttcttacc aagacgttca gtaagagctt tccaaccatc
     1021 ccagtcgttt tcatccatac catcttcaat agtgatgatt gggtatttgt taaccaattc
     1081 ttcaaggtag tcgatttgtt ctgcagatgt acgaacagca gcaccttcac cttcaaattt
     1141 agtgtagtcg taaactttac gttctttatc gtagaattct gatgaagcac agtcaaatcc
     1201 gataaatacg tctttacctg gtacatatcc agcagcttca atcgcagcaa ggatagtttc
     1261 aacaccatct tcagttcctt cgaaacgagg agcgaatcca ccttcgtcac ctacggcagt
     1321 ttccaaacca cgtgatttaa ggattttctt aagagcgtgg aagatttcag caccgtaacg
     1381 aagggcttct ttaaatgttg gcgcaccaac tggcaagatc atgaactctt ggaaagcgat
     1441 tggagcgtca gagtgagaac caccgttgat gatgttcatc attggagttg gaagaacttt
     1501 agtgttgaat ccaccaaggt agctgtaaag tgggatttca aggtagtcag cagcagcacg
     1561 agctacagcg atagacacac cgaggattgc attcgcaccc aatttacctt tgttaggagt
     1621 accgtcaagt gcaatcatag cacggtcaat agcttgttga tcacgtacat cgtagccaat
     1681 gatagcttca gcaatgatgt tgtttacgtt gtcaacagct ttttgtgtac caagaccacc
     1741 gtaacgagat ttgtcaccgt cgcgaagttc aactgcttcg tgttcaccag tagaagctcc
     1801 tgatggaacc ataccacgtc cgaaagcacc tgattcagtg taaacttcta cttcaagtgt
     1861 tgggttaccg cgtgagtcta ggacttcgcg agcgtaaaca tcagtaataa ttgacatttt
     1921 ttactctcct tatgagttaa attttttaca cctctataat accttaaaac ccctcctttt
     1981 tcaagaaaaa acgttatctt tgtgcaactt ttccttaact ttataaagta atcgctttct
     2041 tttgtctgtt ttattctaac ttttatgata tactgttttc atgacagatt tatcaaaaca
     2101 attacttgaa aaagctcatg gtgggttaaa aataaatccg gatgagcaaa gacgctatct
     2161 tggtactttt gaggaaagag ttcttggata tgtagatatt gacacagcaa atagccctca
     2221 gttagaaaaa ggctttttat ttattttaga aaaccttcag gaaaaagcag agccactatt
     2281 tgtgaagatt tcaccaacta tcgaatttga taagcaagtt ttctacttaa aagaagcaaa
     2341 agaaactgat agtcaagcca ccatagtatc tgaagagcat attacttctc cttttggcct
     2401 ggttattcat agcaatgcac cagttcaagt agaagaaaaa gaccttcgac ttgcttttcc
     2461 aaaactttgg gaagttaaaa aggaagaacc agccaaaaca tccttatgga agaaatggtt
     2521 tagctaaatc ttgcacatat ttaataagtg cccaatattg gcagccgtgc gctccagata
     2581 gagactggca tttttcaaac tatcttctaa aggttcactt ttctccaaaa tagaaaagac
     2641 agcttggata ttttcaaatg gtaggggagg taaatcttca gcaagactac cgcaaatagc
     2701 aataacagga actccaacag gggttctttt tgcaacacct ataggcgctt tcccagcaaa
     2761 gctttgacta tcaagtcttc cttctccaac aacaaccaag tcagcatctg aaactttctt
     2821 atcaaagttg attaagtcca agcaggtatc aattccagac acgatacttg cctgagcaaa
     2881 ggcacacaaa ccaccagcaa ggcctccacc tgctcctgct cctttaattt ctaatgttgc
     2941 aggtgagaat ttttcataaa aatcttggat cgcctgatct acgactgcaa acatagtcgg
     3001 atgtagacct ttttgattgc caaaagtgta agtcgcacct tgatgaccac ataagggact
     3061 cacgacatct gctaaaatat gaatttgaac accttcagga attttatagc aattttctgt
     3121 tgaaacagaa gctaagttta ataaggattg accggaagca ggcaagacat ttccatccct
     3181 atcataaaat tgataaccta aaccagcagc aatccccagt cctccatcat tactggccgt
     3241 gccaccaaca ccgatataaa tatctttaat ccctttagaa atgagatgga gaatcaactc
     3301 tccaatacca caagtttgga tttgaagtgg atttcgtttc tctagcggaa tttttccaag
     3361 accaaccaag tcagctactt caaatagtgc cagttcccct ttttgaaaat agcgcatggc
     3421 ttctttttgt ccaaaagggt ctgtcacttg gatccatttt tcttttaggt caagagaatg
     3481 tcggatagca tctacagtac cttctccccc atcaccaaca gggcagagga gacattctac
     3541 atctgctatc gattgttgga agcctctttt tattgcttca gctacctgtt gagctgtcaa
     3601 gctttcctta aacgaatccg gtgcaattac aatcttcata ttttccctca ttctaaacag
     3661 tcaatcaaag gaagaacttc taaaaaatcc ctcttgtcaa catgatgtgg tatttctttt
     3721 ttgagcactt ctttggcaca aaaggcgatt cctaactccg ccgacttcaa cattaataga
     3781 ttattgaccc catcaccgat tgccaccgtt ctttctttag aaagttttag tttctttctc
     3841 catttttcca gagtctcttt tttgacctgg ggacttataa tttgttcaac taattttcct
     3901 gttaaaagac cttctttgac ttcaagctgg ttggcagtga aataggcaat accaagggat
     3961 tttgctaatc tctccaacta ttggtatcca ccagacacca cacaccagac caactaggat
     4021 gccattcttt tggagaatag agatgaactc tgggacattt agcgatagat gaattgagtt
     4081 gaagacgtta tcaaagacca aaataggaag accttccaac aaggacactc tttttcttaa
     4141 actgctttca aagaccaact ctcctcgcat tgcccgactt gtaatctgcg aaatttccgc
     4201 ctcatgacct gcctctctcc ctaaaagatc aatcacttct tctaggatta aggttccatc
     4261 tacatccaaa acacacaagc cttttacttg agacatcagt tctcctctct aaacagccta
     4321 aaaatcgtat gaagtcatca tacgatttta tctattaatt aactaaacta tggtacaagt
     4381 caaggtatga cttgcaggct gtatcccatg agaagtcact ctccatagct tgtttttgta
     4441 ggtttctcca aatgtctgga tggtttctat acaagtccaa tgctgtttgg aaagtccaat
     4501 ttaaccaata aggagataga ttgtcaaagc taaagccagt accgcttcct tcgattggat
     4561 tgaaagcgcg aactgtatct cgcaagcctc caacttcatg gaccaatggc aaggttccat
     4621 aacgcatagc catcatttga gacaagccac acggttcaaa acgacttggc atgaggaaga
     4681 ggtcacaagc agcgtagatt tcctgagcaa gtttgacatc aaaagtgata tttgttgata
     4741 gcttgtctgg gtaaatctga gcaaaccatg agaaagctcc ttcaaaggct ggatcgccag
     4801 ttcccaaaag aacaatctga acatcttctt gcaagatatg gtgaagactt tcgaccacca
     4861 catcaaaacc tttttgacgt gtcaaacgag aaacaattcc caccagtgga acgtctgctc
     4921 taacaggcaa gccaactctt tcttgcaatt ttgccttatt tttggctttc ccagacaaat
     4981 cttcctgatt gaaatgatag tctaaaagag catccgtctg aggattataa agatcagcat
     5041 caatcccatt cacgatacca gatactttac cagactccat tttaagaatc tgatccaaat
     5101 tacatccaaa ctgactagtc ataatttcat gagcatagct aggtgaaacg gttgaaacac
     5161 ggttcgcata gagaatacct gccttcatcc agttcagaca gttgttccat cgaagggtgc
     5221 catcagcgta acgttcaagg ccaactccaa acaaatcacc caacattcct tctgaaaatt
     5281 gtccttggaa ttctaaatta tgaatggtta aaactgtttc aatgtcctca taggcttgaa
     5341 tccaacggta tttttccttc aacaagaaag gaatcatagc tgtatggtag tcatgaacat
     5401 ggagaagatc aggaataaag tcaatccttt ccatagcctc aatggcagcc agttggaaaa
     5461 aggcaaagcg ttctccgtca tcaaaatcac cgtaaacatg accacggaag aaataatatt
     5521 gattgtcaat aaagtagaag gttacaccat ttaatactgt tttcttaatt ccacaatact
     5581 gtctgcgcca accaacgctc acctcaaaat gaagcacatc ttcaatctga tttccaaatt
     5641 tagcctctac catatcatag tagggtaaaa tcactgcaac ttcgtgccca gcttttacca
     5701 gtgattttgg aagagcgcca atgacgtctc ccaaaccacc tgtttttgaa aagggtgcac
     5761 cctctgctgc tacaaataaa attttcatga atgaatatcc tctgttactt tagcaccttt
     5821 cttaaccaca actggatgtt ctgcagttcc tcgaatcaca acaccaggct caacttcaac
     5881 ccctttgtcc aagatagcat attcgacctg agccccttct ccaataacaa cacgagggaa
     5941 gagcaggcta tctttaacca agctatcctt atggacatga atattacgtg atagaacaga
     6001 attagctact tgaccttcaa taatactacc agaggcaaac tgagaagtgc ttaccttaga
     6061 tgtattagca tagtaagttg gctcttcgtt tttgaccttt gtataaatct tttggtttgg
     6121 tgagaaaaga gaatagaatt tttgtgattc aagcatatcg atattcgctt gataataaga
     6181 tttaacagag tgaatattgg ctagatagcc tgtgtactcg taggcgaaag ctccctcttt
     6241 tacagccaaa tcccgtaaaa catagcgcaa tttctctgga tgttcttttt tagcttcttc
     6301 ttccaagtgt tcaatcaacc aaggtgtatc aacgacaaag atatctgtag acatattgaa
     6361 cgtttcagct gttgacttgc tatcaaagag tttatgagaa agaacatggt ctgtttcatc
     6421 tacatccaag attgcattta cttctgaaat atctttctta gctagttttt tataaactac
     6481 agtgataggc tcttttgttg tactatgtag gtggaaaact tggttcaaat caatgttaat
     6541 aagaacatcg cagttgaggg caaccgtttg gtttgagcca gaacgtttca aataagtaag
     6601 aagctgttgg tagtattctt ttccaactgt actactttct acacgggtat tgtaaattcc
     6661 tagatagtaa tgactaagaa gggttgataa gccccactcg cgtcctgaac gaatatggtc
     6721 aaatactgag ctgatattat cctgctggaa aataccaaag acactacgaa cacctgcatt
     6781 agcaaggctt gaaagtggga agtcaatcaa acgatatttc ccaccaaatg gcaaacttgc
     6841 tactggacgg tggtccgtca atgtcgacat attgtgaaaa ccaactgtat ttcctaaaat
     6901 ggcagaatat ttatcaatct tcatctgttg ctacccccac tacttcatta tatcctacaa
     6961 cttgtacttc atctgttcca tcaatttcga caccgtcaga aataatcgca ccttcaccaa
     7021 taatggcacg tttaatctta gctccttgac caataatagc tccactcatg ataactgaat
     7081 caaggacttc cgctccttcg cgaacttgcg cgcctgttga aaggatagaa tgtttaatag
     7141 ttccatcaac gaaacatccg tctacaacta atgagtcttc cacatgagca tttgccccga
     7201 ggaagtttgg tggtgaaatc aagtttcttg agtaaatctt ccattgacgg ttacgactat
     7261 ccaaggcatt ttctggagaa atatactcca tgttcgcttc ccaaagtgac tcaatagtac
     7321 caacatcttt ccaataacca ctaaattcgt aagcataaac actttcacct gactcaaggt
     7381 aatttggaat gacattttta ccaaagtctg acatgccaac cttgctcttt tcagcagcga
     7441 ctaacatatt acgaaggcgt tgccaatcaa aaatgtagat tcccatagaa gcttttgtag
     7501 atttaggttg agctggtttt tcttcaaatt caacaatacg attgttagca tctgtgttca
     7561 tgataccaaa acggatcgct tctttaagag ggacgtctaa aactgctact gtcaagctgg
     7621 cattattatc cttatgagac tggagcatat catcatagtc cattttgtag atgtggtccc
     7681 cagacaaaat caagacatac tcaggattga cactgtcgat atagtcgata ttttggtaaa
     7741 tagcgtgact agttccctca aaccaacgat ttccttcact tgcagaataa ggttgaagaa
     7801 tagagacacc tgaattaata ccgtctagtc cccagcttga accattccca atatggttgt
     7861 tgagagcaag tggttgatac tgtgtaacca ccccaacatt atgaatccct gagttggcac
     7921 agtttgatag ggcaaagtca atgatacggt agcgcccacc aaattgcaca gctggttttg
     7981 cgatgctttg agtgagttta ccgagacgag ttccttgccc accagcaaga atcaaagcta
     8041 acatttcatt tttcattttc tactcctttt tggtttttat ttgtgacggt tttagtagat
     8101 ttcaagcgac gtttgatttt ccatacactt gctcccatag ccggtagggt aaaggttaag
     8161 gtctgctcat aatctttcca taggccttct tgcgtttgaa cagtttgatt atgttctttc
     8221 caaacgcctc cccactcttc caactcagta ttccatactt cttcgtaaat tcctgcaacg
     8281 ggtagtccga ttgtaaaatc tttccgctca acaggtacca tgttaaagat acagactaac
     8341 atttctccct ttttaccctt acgaataaag gaaagaacac tctggtctcg attatccgca
     8401 tcaatgattt caataccatc atagctggta tcgatttccc acagacagcg atgatctttg
     8461 taaaactggt ttagctgaga agtgaaatac ttcatcttag cattcattgg gtcttctagg
     8521 ttagaccatt ccaactgttc ttcagatttc cattctagga attgaccgta ttcgctaccc
     8581 atgaagagca atttcttacc agggtgacaa atttggtacg tatagagatt gcgcaagcct
     8641 gcgaattgat tgtaacgatc tccccacatc ttatgcatca tactcttctt gccatgaacc
     8701 acttcatcgt gcgagaatgg caagagataa ttctccttga aaacatacat aaagctgaaa
     8761 gtcaccaggt taaagtcata tttacgataa atcggatctt cttcgtagaa acggaggata
     8821 tcattcatcc agcccatgtt ccatttgtag tcaaatccta gaccaccaat ctctttcatt
     8881 cccgtaatct tggtcgcaga cgaactttct tctgcaatca tcatcacatc tggatattct
     8941 aacttaataa cttcattcaa gcgttgaaga aaataataac cttcatagtt gagatttccg
     9001 ccatctttat taggtgtcca tggagcatta tcatagtcca aatagagcat gttgctaaca
     9061 gcatccacac gaataccatc caaatgatag acatcaatcc aatgcttaat gcaagaaatt
     9121 aagaaggact ggacttcatt ttttccaagg tcaaaattaa gggcacccca accatggtta
     9181 tgagccttat tatggtcttg gtattcaaaa gtcggtgtcc catcataata ggctaaggca
     9241 tcatcgttga tggtaaagtg acctggtacc cagtccacaa taaccccaat attatgggta
     9301 tgacactcct cgacaaaatc ttgaaactcc tctggtctgc cataagcatg ctctaaagcg
     9361 aagtaaccca taagctgata cccccaactc aagcccaaag gatgggacat caagggcata
     9421 aactcaatat gagtatagtt catttcaacg agataaggaa tgagttcatc cttgagctgg
     9481 gcaaaactat aaggactgcc atcagaattt cttttccatg atccagcgtg aacttcataa
     9541 atattgacag gacgctcttc aaagccccaa cgttttcttc gtgccagcca aagtccatcc
     9601 ttccatttct tctcaggaag ctctgttaag attgcccctg ttcctggacg agcctcatac
     9661 ctgacagcaa aagggtcaat cttcatcagt tgatgaccat tttgacgtgt gacatgatat
     9721 ttgtaaatat gcccttcttg agccatattg gtaaagactt cccagacccc aaaatcattt
     9781 cttaccattg gaatctgatt ttcaatccag ttggtaaaat caccaaccaa gtgaacagcc
     9841 tgagcattag gtgcccaaac acggaaggta tagccatgct ctccatttag ttcttcccta
     9901 tgtgctccta gataatgttg gagataaaaa ttttcacccg tcataaaggt ttttaatgct
     9961 tctctattat ccatatactc cccttctcct gtaagcgttt tctatgtttt tattatacta
    10021 cctttttaga gaagattcaa gtaaattact atacttcttt aattattttg aaaatctaca
    10081 acaagttcac ttactcgttc aattgtaaat caatattttt tcaaaaaatt gcgaaaacg
//
LOCUS       DAVKJD010000063         9730 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKJD010000063 DAVKJD010000000
VERSION     DAVKJD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.063"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            253..348
                     /locus_tag="VW157_001584"
                     /pseudo
     CDS             253..348
                     /locus_tag="VW157_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(601..1221)
                     /locus_tag="VW157_001585"
     CDS             complement(601..1221)
                     /locus_tag="VW157_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9552858.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1221..1703)
                     /locus_tag="VW157_001586"
     CDS             complement(1221..1703)
                     /locus_tag="VW157_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9552859.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1703..2545)
                     /locus_tag="VW157_001587"
     CDS             complement(1703..2545)
                     /locus_tag="VW157_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9552860.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2640..3428)
                     /locus_tag="VW157_001588"
     CDS             complement(2640..3428)
                     /locus_tag="VW157_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9552861.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQALK
                     HLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDKAYKS
                     HITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTMSNMN
                     EGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLESSPKT
                     DLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3572..4951
                     /gene="glmU"
                     /locus_tag="VW157_001589"
     CDS             3572..4951
                     /gene="glmU"
                     /locus_tag="VW157_001589"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9552862.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLTGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4961..5506
                     /locus_tag="VW157_001590"
     CDS             4961..5506
                     /locus_tag="VW157_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9552863.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5518..5832
                     /locus_tag="VW157_001591"
     CDS             5518..5832
                     /locus_tag="VW157_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9552864.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5849..6541
                     /locus_tag="VW157_001592"
     CDS             5849..6541
                     /locus_tag="VW157_001592"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9552865.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6618..7109
                     /gene="rocS"
                     /locus_tag="VW157_001593"
     CDS             6618..7109
                     /gene="rocS"
                     /locus_tag="VW157_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9552866.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7207..7797
                     /locus_tag="VW157_001594"
     CDS             7207..7797
                     /locus_tag="VW157_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9552867.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7806..9569
                     /locus_tag="VW157_001595"
     CDS             7806..9569
                     /locus_tag="VW157_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9552868.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3003 a         1686 c         2075 g         2966 t
ORIGIN      
        1 ttttctgtct aacttttggg gtgtagttca tgtacacctg atatgatgcg ttttataatt
       61 ttaaagactt tttgaccagc ctcatttttt taacttgata ctcagtgaaa agcaaagatt
      121 aaactaggaa gctagctgta ggctgctcaa agaacagctt tgaggttgca gataaaactt
      181 gtgaggtaac caacatatat aatgtgaagc tgacgtggtt tgaatagatt ttagaagggt
      241 atgagtctgg aagttttaat ggataatgca agattccata gaatgggtaa gctagagttc
      301 ttatgtgaag agtttgggca taaactttta ccttttcctc cctactcatc ttagtataga
      361 aaagtgaatc taaaatagta cataactgct tctaaaacat tcttataaat tgatttaaat
      421 tctcaaatca tattattcag ttcttatttc attttgctct acaatcctgt tgagaagaca
      481 cgtgttcata tcaaaaaggt attggcaagt tgcaatacct ttttacaagg ttcttttgtc
      541 ttatttttgt ttcaactgac tatatctcct atggttctag ttcagaaggc taggctataa
      601 ttatgattga taagaagtat cattccaagt attgagagtg aatgtttcaa aatcatgggt
      661 ttctataatg gtcaggctgg catttgctag accgccatct ttacgaagaa gtggttcttt
      721 atagcctagg agagtacgaa gactggcagt aagattggcg ccgtgtccga caattagaat
      781 acgctcagct ggactatctt ttaatgattt gataaattgg atggtccgct gagttgtact
      841 atagagggat tcggctccga acattcgagt gtcaaattga gcaagatttg aacgaaaagc
      901 ctggatttgt tgcgggtaaa tagcttccag ggttgcaatt ttcaaacctt ctaacttccc
      961 aagttgccat tcacggagat taggaacgat ttctaaagaa cagggggtat ggagttgact
     1021 ttggataatc tcagcagatt tgaccgctcg aggtaaatca cttgaataaa tctgatcaaa
     1081 aggaatttcc ttgagatact ggccaagtcg ttttagggtt tcaatggatt caggaagaag
     1141 aggagaatca ccgctagcac cttgaaaacg accttcttgg ttccagaggg tacgaccgtg
     1201 gcggacaaag tagagtttca ttacttgatg tcctccaaaa tatctacaaa gtctgccttt
     1261 acaaagctag ccaagtcttg tggcgcgacg ataatgctgt gtccgacttc gcctgcagag
     1321 acaatcattc gatccaaatc tagagcaatt ttatcgataa aaatgggata attgtgtttc
     1381 tgacgaattc cgacaggatt attggctcca tgaatgtaac cagttgtttt ttctaagtcc
     1441 ttttgtggaa tcatgctcac ttttttattg ccagaaattt tagctagttt cttttcagac
     1501 aagtgctgag tgatagggac aattccgata atcggtccgg tcttgtctcc caaaagcgcc
     1561 aaggttttga aaatctgatc tcgttcataa ccttgaggaa gctctccttc tagggcattg
     1621 atttgaatcc cctgatgagg gatagctgct ttagatagga tttgttccac caatgttttt
     1681 ttgattttaa ctttttttgc cattatttat atttatcctc caattgactc atccaaatac
     1741 caagccagat tcccagcgca aagaagaagg cgatgatgac ataaccgaca agtgaaagtc
     1801 ctgtgtattg gatactttca gcgtttcctg catttggaat taagatcaaa agggtacttg
     1861 ataggacgat accgatgatg aaatgataga cgcgtgagtg gtagttgttt aaggcataat
     1921 ccatcaattt tgaaaaaacg atgagagttg cacctgcacc aattccaatc ggaaagaagg
     1981 ttcccaagaa atcaaaagtt ttaaaaccag tcaacatagg agcatatagt cccaaaatca
     2041 aaagtaaatt tgatgggctg aggccaggaa ccaagacgcc aagggccaat agtgcgcctg
     2101 ctaggacgaa gttaagaaag ctggcgctta aggttccaac gacaaaattt aaggcataga
     2161 gtcctaatcc agaaatgata aaggttgtcc ataaccaagc taaatcaatc ttgtctcggt
     2221 cagattctcg agttgattct ttgaggaggc taggaactgt accgataata gctcccgcaa
     2281 agctccataa tacaaaaacc tgataatttt caagcaggta ttcaatcggg taggaaaata
     2341 agccgattcc cagaagcata ccgatggcaa ctggaataaa gtacaaaaca ttttctttaa
     2401 agtctttaaa gggatgggcc agaaagccaa tcattcgttc atagattcct aagattgctg
     2461 ctagaacccc tccggaaatt cccggtagga taaatccaag agcaattaca atccctttaa
     2521 taacgcgtgc taaccatgag agcatatatt tcctccaact atttctattt caaaaaatcc
     2581 ttactatatt gtatcacaat cagacacaaa aagaaaaagc aaatgataaa caaatgcttt
     2641 taaggtttta aaaaaagttt tcgaaaggtt tcttctttat tttttaaggg agagataacg
     2701 ttgatatcta aatcgtggtc aaagccggca atttttcctt tagatgtgta ttggtgaata
     2761 tcataatcta aatcagtttt aggactgctc tccaaaaatc ctgagtctga gccgtaggac
     2821 ggaatccaaa cagaggtaaa cttgcctgta tcaatactgt gttcttccat gaagtagaca
     2881 ccaacgtaga tgccgatgtt tttagcacct agtgatgcta gttttgctcg aaagttttcg
     2941 acaccttcgt tcatattaga catggttttg tcttccacgt caagccaata gtaactaggg
     3001 ctgtaaggag aggcagcatt gtagaaaact tcggcagcct tttccatttc ttggacactt
     3061 tttccagcta cataagcgta gacagcaact gggacattcc gcttttgaag ttcagtgata
     3121 tgactcttat aggccttgtc tattccattg ataaatgaag catcattttc ttttgttgtt
     3181 tgagcaccac tgtgaacacg aacaatagca cctgaaatat tttgtgagag ggcatcgtag
     3241 ttgatttcct caggacgctg ccagccagag aggtcaataa tcggtttgtc taagtgtttc
     3301 aaagcctgtg ctatattgga ttttgtttta aacgattggc tgtcattaag tgggcgattg
     3361 atgattaaaa tgaacatcat aatcccaaaa aaactaaata aaataagtgg atgaatttgt
     3421 tttctcatat cttataagtc taccctaaaa atcaaaaaaa atcaaaaaaa tgggttaagg
     3481 aagagacttt agagcatttt ttcattcaag agtgcggaat gatttgaaat atggtataat
     3541 aaaagggaat ttctacagaa aagagaagat tatgtcaaat tttgccatta ttttagcagc
     3601 gggtaaaggg actcgcatga aatctgattt gccaaaagtt ttgcacaagg ttgcgggtat
     3661 ttctatgttg gaacatgttt tccgtagtgt gggagctatc caacctgaaa agacagtaac
     3721 agttgtagga cacaaggcag aattggttga ggaggtcttg actggacaga cagaatttgt
     3781 gactcaatct gaacagttgg gaactggtca tgcagttatg atgacagaac ctatcttaga
     3841 aggtttgtca ggacacacct tggtcattgc aggagatact cctttaatca ctggtgaaag
     3901 cttgaaaaac ttgattgatt tccatatcaa tcataaaaat gtggccacta tcttgactgc
     3961 tgaaacggat aatccttttg gctatggacg aattgttcgt aatgacaatg ctgaggttct
     4021 tcgtattgtt gagcagaagg atgctacaga ttttgaaaag caaatcaagg aaatcaacac
     4081 tggaacatac gtctttgaca acgagcgttt gtttgaggct ttgaaaaata tcaataccaa
     4141 taacgctcaa ggcgaatact atattacaga cgtcattggt attttccgtg aaactggtga
     4201 aaaagttggc gcttatactt tgaaagattt tgatgaaagt cttggggtaa atgaccgtgt
     4261 ggcgcttgcg acagctgagt cagttatgcg tcgtcgcatc aatcataaac acatggtcaa
     4321 cggtgttagc tttgtcaatc cagaagcaac ttatatcgat attgatgttg agattgctcc
     4381 ggaagttcaa atcgaagcca atgttacctt gaaagggcaa acgaaaattg gtgctgagac
     4441 tgttttgaca aatggtactt atgtagtgga cagcactatc ggagcaggag cggtcattac
     4501 caattctatg attgaggaaa gtagtgttgc agacggtgtg acagtcggtc cttatgctca
     4561 cattcgtcca aattcaagtc tgggtgccca agttcatatt ggtaactttg ttgaggtgaa
     4621 aggatcttca atcggtgaga ataccaaggc tggtcatttg acttatatcg gaaactgtga
     4681 agtgggaagc aacgttaatt tcggtgctgg aactattaca gtcaactatg acggcaaaaa
     4741 caaatacaag acagtcattg gagacaatgt ctttgttggt tcaaattcaa ccattattgc
     4801 accagtagaa cttggtgaca attccctcgt tggtgctggt tcaactatta ctaaagacgt
     4861 gccagcagat gctattgcta ttggtcgcgg tcgtcagatc aataaagacg aatatgcaac
     4921 acgtcttcct catcatccta agaaccagta ggagcctatc atggagtttg aagaaaaaac
     4981 gcttagccga aaagaaatct atcaaggacc aatatttaaa ctggtccaag atcaggttga
     5041 attaccagaa ggcaagggaa ctgcccaacg ggatttgatt ttccacaatg gggctgtctg
     5101 tgttttagca gtaacggatg aacaaaaact tatcttggtc aagcagtacc gcaaagctat
     5161 cgaggcagtc tcttacgaaa ttccagccgg aaaattggaa gtaggagaaa acacagcccc
     5221 tgtggcagct gcccttcgtg aattagagga agaaacagcc tatacaggga aattagaact
     5281 cttgtacgat ttttattcag ctattggctt ttgtaatgag aagttaaaac tatatttagc
     5341 aagcgattta acaaaagtgg aaaatccgcg tccgcaggat gaggatgaaa ccttggaagt
     5401 ccttgaagtg agcttagaag aagcgaaaga attaatccaa tcaggtcata tttgtgatgc
     5461 caagacaatt atggctgttc agtattggga gttgcagaaa aaatagagga ggtcagtatg
     5521 ggtaaatctt tattaacgga tgaaatgatt gaaagagcta atagaggcga aaaaatttca
     5581 ggtcctcctt tgctagatga taatgaggaa actaagattt taccaacctc ttcttcccgt
     5641 tttggttatg ccaatcctaa ggatcatggt tttagccagg aaaccttgaa gattcaggtc
     5701 gaaccatcta ttcataaaag ccgtcgtatt gaaaatacca agagaaatgt cttcaattct
     5761 aagttgaata aaatcttatt tgcggtcatt tttctcttga ttttgcttgt tttagcaatg
     5821 aaacttttgt aatagaaaag gaattgaaat gaaaatagga attattgctg ctatgccaga
     5881 agaactggct tatctggtcc agcatttaga taatacccag gagcaagttg ttttggggaa
     5941 tacctatcat acaggaacca ttgcttctca tgaagtcgtt cttgtagaaa gtggaattgg
     6001 taaggtcatg tctgctatga gtgtggcgat tttggctgat catttccagg tggatgccct
     6061 tattaatacg ggttcagctg gggcagtagc agaaggtatc gctgttgggg atgtcgtgat
     6121 tgctgacaaa ttagcctatc atgacgtgga tgtcacagct tttggctatg cttatggaca
     6181 aatggcgcaa caaccgcttt atttcgaatc agacaaaacc tttgttgctc aaatccaaga
     6241 gagtttatct caattggacc aaaactggca tcttggtttg attgctacag gagatagttt
     6301 tgttgcagga aatgacaaga tagaagcgat taagtcccat ttcccagaag ttttagccgt
     6361 ggagatggag ggggcagcta ttgctcaagc agcgcatgcc ctcaatctcc cagtcttagt
     6421 catcagagct atgagtgaca atgccaacca tgaagcaaac atcttttttg atgagtttat
     6481 tatcgaagct ggacgtcgct ctgcccaagt cttgttgacc tttttgaagg ctttagatta
     6541 agcggaaatt tgacagtttt tctagcttat gataagattt aagtaaagaa aagctagaaa
     6601 acgtttcaga ggatattatg agtattgaaa tgaccgtcag tgagattgca gaggtcttag
     6661 gattatctcg ccaagcaatc aataaccgtg tcaaagaatt accagaagaa gacacagata
     6721 aaaatgacaa aggggtaaca gttgttacca gaagtggctt gattaagcta gaagaaatct
     6781 ataaaaaaac gatttttgaa gatgagcctg tcagtgaaga tgtcaaacaa cgtgaactga
     6841 tggagattct agtggatgag aagaatgcag aaattcttcg tctttatgaa caattaaaag
     6901 ccaaggatcg tcagttatca gaaaaagacg agcagatgcg tatcaaagac cgtcagattg
     6961 ctgaaaaaga caaacaatta gatcagcaac aacaattgac ccttcaagcc atgaaggatc
     7021 aagaaaatct taagctagag ctggaccaag caaaagaaga agtccaatcc actaagaaag
     7081 gcttttttgc tcgtttattt ggaggataat caaggagctg tttaggttaa atgctaacct
     7141 gatggcttct tttgtttgtg aatagtatag ttgattagaa cagagacagt agaggagagg
     7201 tacagaatgg aacgattaga agattacatt gcttttgact tagaatttaa tcagcacgaa
     7261 ggacaaacac acttgattca agtatcggca gtgcgtttta gagatggtcg ggaaatcgat
     7321 gcctatgatt cctatgttca taccagcgtc cctctaaaaa gctttatcaa tggtttgacc
     7381 gggattacag ctgagacctt aaaagatgct cctccagttg agaaagtctt agaagaattc
     7441 caagagtttg tgggttcttt gctgatgata ggctacaatg ctgctaaaag tgatttacct
     7501 attttagcag agcatggttt agactatagc caacagtaca aggtggattt gtatgatgaa
     7561 gcttttgaac gtcggagttc agatttacat ggtattgcca atctcaaatt gcaaactgtt
     7621 gcctcatttc ttggatttca aggtcagtct cataatagtt tagaggatgc tcggatgacg
     7681 gcgcgtgttt acgaatcctt cttagagtcg gatcaagcta gggaattgtt agagacagaa
     7741 agtagcttat caaacaatcc ttttggtggc ttggacttgt ctcaattatt tgactaggag
     7801 tgcctatgaa acctgaaaaa cctaaaaaat tgggtcttaa acagatttac ctcaatcttg
     7861 ataaaatcct ctttctattt ttcttgattt ttatgatagt ggagtatttg tgggtaccat
     7921 tcaattcatg gttggctagc ttcttattag atcagacagg ttatcttttt atttcctata
     7981 acaatgtctt agccatcctt actcattctc cattgattag tctagctttt ttagccttga
     8041 ttgtaattaa ccttctggtt gcttattttc aaatagggct cctctttatt ggtgctcgtc
     8101 atctcctcta taatgaaaag cgaaggctaa ttgagtatat ccgcaaggtt ttccatgaaa
     8161 gttttggatt tatgaaaaag ctaacttgcg ctaaagcctt gtttatcttt ttctatatag
     8221 ctatgctttt tccttttata cggaaaatgc tgaagattta ttatcttaat aaaatcgtca
     8281 tcccagagtt tatccaagct tatttagaag ataaatattg gatgtggtgg ctgagcatcc
     8341 tacttctatc tcttatcttt ctctatgtct ctgtcagatt gatgtttgcc cttccgaaga
     8401 ttgtatatga tcagctgact gttcgagaag cagtgatgtt tagcttggag aaaaccaaga
     8461 agagagttac tttttatgct tggaatttat tttacatctt gctcaaagca catttattat
     8521 tttatcttcc tttgattcct ttattattgg ctcaaacttt ggtagatgat atcactcaga
     8581 gagagtctct gattcttggt attttgaatt ttgtcgtgat taaaaatctc tattatatgg
     8641 ctctgactta ttttttggtt aaatttgttt catttttgac aggaaaggag ttggatatgc
     8701 ttcctaggag agaaaaagat cacatcgtgc gatggggcgt catgacttgt gccagtcttt
     8761 tctttgcctt agaaggttat atttatctgg aggctcccat ggttcatcta cctcaactta
     8821 tttctcaccg aggggtttcc aatgcaaatg ggattcaaaa tacagtagag tccttggaaa
     8881 ctacagcaca actcaaacca gacttggtgg agacggacgt gcaggaaaca aaagatgggc
     8941 agtttgtcat gatgcatgat gctaacttga aaaatttagc aggtatcaat aaaagtcctc
     9001 aagacttaaa cttggaggag cttaaaggga ttgatatttt tgaaaatggc taccagacta
     9061 aaatttcaag ctttgaagat tatctcagtc gagccaacga acttggtcaa aaattactaa
     9121 ttgaaattaa aaccagtaaa aaagatagtc cagacatgat gaaccgcttt ttagcccgtt
     9181 atgctgcaaa gctcaagatt tatggacatc aaatccagtc tttagactac catgttgtcg
     9241 aaaaagtaag acagtatgat gccgaactgc cagtttattt catcatgccc tacaattctg
     9301 tctttcctaa aacaagagcg acaggatata ctatggagta ctcaaccttg gatgaatatt
     9361 ttgtaagcaa actatggaca acggatcaga aactttatgt ctggactgtc aatgattcag
     9421 aagctatcag caaatctctt cacttaggag tagatggagt gattacagat gatttggaaa
     9481 aagttcagcg ggaaatagaa gtagcccaag aagatccaga atatacggat ttgctcttga
     9541 aaaaagcatt ggaattcttt gaattttagt aataaaaaag aggaacggtt gttcctccca
     9601 tgataggtaa gtattcttgt gaatgcttgc ttttttgtgg ttgcaatccg tttacgagaa
     9661 ttgtttgtat tgttcttgtt tcaatttact ttatagtgct ttgaagttgg aatgctacac
     9721 tataacttct
//
LOCUS       DAVKJD010000064         9359 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKJD010000064 DAVKJD010000000
VERSION     DAVKJD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.064"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            462..1505
                     /locus_tag="VW157_001596"
     CDS             462..1505
                     /locus_tag="VW157_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9552869.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            1672..3759
                     /gene="topA"
                     /locus_tag="VW157_001597"
     CDS             1672..3759
                     /gene="topA"
                     /locus_tag="VW157_001597"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9552870.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEVASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3865..4224
                     /locus_tag="VW157_001598"
     CDS             3865..4224
                     /locus_tag="VW157_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9552871.1"
                     /translation="MRLIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4317..4949
                     /locus_tag="VW157_001599"
     CDS             4317..4949
                     /locus_tag="VW157_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9552872.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQLGVTQLHGTKVVF"
     gene            4993..5649
                     /locus_tag="VW157_001600"
     CDS             4993..5649
                     /locus_tag="VW157_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9552873.1"
                     /translation="MFEIFKSYQFNQEKVHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5668..6300
                     /locus_tag="VW157_001601"
     CDS             5668..6300
                     /locus_tag="VW157_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU9552874.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNICQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6328..6762
                     /locus_tag="VW157_001602"
     CDS             6328..6762
                     /locus_tag="VW157_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU9552875.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6774..7811
                     /gene="leuB"
                     /locus_tag="VW157_001603"
     CDS             6774..7811
                     /gene="leuB"
                     /locus_tag="VW157_001603"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU9552876.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLVAIGSPQYDGVAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERIAGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKILISIDKQNVLATSKLWRKVAEEVAQDFSDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSV
                     PDIAGQGIANPISMILSVVMMLRDSFGRYEDTERIKRAVETSLAAGILTRDIGGQAST
                     KEMMEAIIARL"
     gene            7808..8077
                     /locus_tag="VW157_001604"
     CDS             7808..8077
                     /locus_tag="VW157_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9552877.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8081..8440
                     /gene="leuD"
                     /locus_tag="VW157_001605"
     CDS             8081..8440
                     /gene="leuD"
                     /locus_tag="VW157_001605"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9552878.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSKWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8602..9225
                     /locus_tag="VW157_001606"
                     /pseudo
     CDS             8602..9225
                     /locus_tag="VW157_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         2899 a         1641 c         2072 g         2747 t
ORIGIN      
        1 acactataat ttagtgtaat tgagaaagga gaaatgattg tgattgatgt tggctaggtt
       61 attttcaatg attcctaccg tctcaaatct tgtcagtaag gaaaaataaa ttcttcaaaa
      121 gtagagatta caaggcttgt ttaagaaaga attcaaagac cttgacaaat aaaaataaaa
      181 tggttattat aagaaatggt ctaaaataga tgatgatact tttcgaaaat ctttttacgt
      241 cagctcagct ttgccttgct gtgttttgag caagctacgg ttagcttccg agtttgattt
      301 tcatttacta gaaatgaaac tgatgagaga tatcagtaga catttgagtc aggatattat
      361 ggaaaatgat aaaaagagct cgtgagattg gcatatcaga ctactaaagt attgagtttg
      421 ttaggatttt agcgactagt tagctgggaa aggaagatat tgtgacaaat aataaactgt
      481 attcgttgat agaatttaga aataaaatat atgaagaatt agaactttcc agaagtgatt
      541 tagcgatttt actatgtgcc atgcttatcg cctctatcgg attaaatatg gattcgactc
      601 ccgtgattat tggagccatg ttaatctctc ctttgatgac acctattctg ggagtggggc
      661 tctctctagc tatatttgat tttaaattgt taagaaaatc ttttaaaata ttagctattc
      721 aaattcttgc cagtttaata gcttcaacac tttattttta tctttctccc atttcgtatg
      781 ctagttcgga aattgttgct agaacctctc cgactatttg ggatgttctc attgcttttg
      841 taggagggat agcaggtatt attggtgcta ggaaaaaaga gaccaataat attgttcctg
      901 gtgttgctat tgcaaccgcc ttgatgcctc ctctttgtac agtaggttat gctattgctt
      961 ctgctaatct aaaatttatc ataggctcct cttacctatt cctcatcaat tgtagcttta
     1021 ttgtcattgc gacttatata ggtgttaggt tgatgatggt taagaaacat tattttaaag
     1081 ataatgaaga agactctaaa atgcgtagga ttttgcttct agttgctgtt ttgctgatga
     1141 ttccgagttt catctctgca acgactttag tgagagaaac gttgaaaaaa gagtccctta
     1201 agaaatttat atcagagcag tttcaggggc ataatatttt gaaaaaaacc tattctaaaa
     1261 agactcatac cctaaagcta accatttcag gaaattattt gacagaagaa gaactcgata
     1321 tgatttcaag taagagaggt gactatggtt taagtgatgt ttctgttcaa gtttcacaat
     1381 tgtctgattc agaacaactt agtaaggaag aactggtgga gtatttcttc cagtatatca
     1441 aggataagga agcaaaagaa aaggaaaaag ctaataagtt ttatacagag tctgaggagc
     1501 aataatttct tgagaatagc tggtttttct cgtgagtctt ctatgtatat caaaggaaga
     1561 ctgaggtttt aagtataaaa cttttcttct attatagtag ctaagtcttg acagggttca
     1621 aaaaatggtt tacactttat aaagtttatt actttgaaaa ggtgtgatac tgtggctacg
     1681 gcaacaaaaa agaaaaaatc aacagttaaa aaaaatctag tcatcgtgga gtcgcctgct
     1741 aaggccaaga cgattgaaaa atatctaggc agaaactaca aggttttagc cagtgtcggg
     1801 catatccgtg atttgaagaa atccagtatg tccgtcgata ttgaaaataa ttatgaaccg
     1861 caatatatta atatccgagg aaaaggccct cttatcaatg acttgaaaaa agaagctaaa
     1921 aaagctaata aagtttttct cgcgagtgac ccggaccgtg aaggagaagc gatttcttgg
     1981 catttggccc atattctcaa cttggatgaa aatgatgcca accgtgtggt cttcaatgaa
     2041 atcaccaagg atgcagtcaa aaatgctttt aaagaacctc gtaagatcga tatggacttg
     2101 gtcgatgccc aacaagctcg tcggatcttg gaccgcttgg tagggtattc gatttcgcct
     2161 attttgtgga agaaggtcaa gaagggcttg tcagcaggtc gcgttcagtc cattgccctt
     2221 aaactcatca ttgaccgtga aaatgaaatc aatgccttcc agccagaaga atactggaca
     2281 gttgatgctg tctttaaaaa gggaaccaaa caatttcatg cttccttcta tggagtagat
     2341 ggtaaaaaga tgaaactgac cagcaatgac gaagtcaagg aagtcttgtc tcgtctgacg
     2401 agtaaagact tttcagtaga tcaggtggat aagaaagagc gcaagcgcaa tgctccttta
     2461 ccctatacca cttcatctat gcagatggat gctgccaata aaatcaattt ccgtactcga
     2521 aaaaccatga tggttgccca acagctctat gaaggaatta atatcggttc tggtgttcaa
     2581 ggtttgatta cctatatgcg taccgattcg actcgtatca gtcctgtagc gcaaaatgag
     2641 gtggcaagct tcattacgga tcgttttggt agtaagtatt ctaagcacgg tagcaaggtc
     2701 aaaaacgcat caggtgctca ggatgcccat gaggctattc gtccgtcaag tgtctttaat
     2761 acaccagaaa gcatcgctaa gtatctggac aaggatcagc tcaagctata tacccttatc
     2821 tggaatcgtt ttgtggctag ccagatgaca gcggccgttt ttgataccat ggctgttaaa
     2881 ttgtctcaaa aaggggttca atttgctgcc aatggtagtc aggttaagtt tgatggttat
     2941 cttgccattt ataatgattc tgacaagaat aagatgttac cggacatggt tgttggagat
     3001 gtggtcaaac aggtcaatag caaaccagag caacatttca cccaaccgcc tgcccgttat
     3061 tctgaagcaa cactgattaa aaccttagag gaaaatgggg ttggacgtcc atcaacctac
     3121 gcgccaacca ttgaaaccat tcagaaacgt tattatgttc gcctggcagc caaacgtttt
     3181 gaaccgacag agttgggaga aattgtcaat aagctcatcg ttgaatattt cccagatatc
     3241 gtaaacgtga ccttcacagc tgaaatggaa ggtaaactgg atgatgtcga agttggaaaa
     3301 gagcagtggc gacgggtcat tgatgccttt tacaaaccat tctctaaaga agttgccaag
     3361 gctgaagaag aaatggaaaa aatccagatt aaggatgaac cagctggatt tgactgtgaa
     3421 gtgtgtggca gtccaatggt cattaaactt ggtcgttttg gtaaattcta cgcttgtagc
     3481 aatttcccag attgccgtca tacccaagca atcgtgaaag agattggtgt tgagtgtcca
     3541 agctgtcatc agggacaaat tattgagcga aaaaccaagc gtaatcgcct cttctatggt
     3601 tgcaatcgct atccagaatg tgaatttacc tcttgggaca agcctgttgg tcgtgactgt
     3661 ccaaaatgtg gcaacttcct catggagaaa aaagtccgtg gtggtggcaa gcaggttgtt
     3721 tgtagcaaag gcgactacga ggaagaaaag attaaataag aggaggggcc cgaaagtaaa
     3781 tttcaggctc cttttggtta gaacttgaca aaatacatca ttttatgcga aactagaaga
     3841 agattatttt aactaggaga agttatgcgt cttatctatc tgataattgg ttttttatca
     3901 ctgaccttgg ctattgttgg ggttgtttta cccttgttgc cgacaacgcc tttccttttg
     3961 ttgtctattg cttgtttctc cagaagttcc aagcgtttcg aagactggct ttatcatacc
     4021 aagctctatc aaacatatgt agctgatttt cgcgagacta agtctattac gcgtgaacga
     4081 aagaaaaaaa tcatcgtctc tatctacgtc ttgatgggaa tttctattta ttttgcacct
     4141 cttttaccag tcaaaatcgg tctgggtgct ttgaccatct ttatcactta ttatcttttc
     4201 aaggtcattc cagacaaaga atagttaaaa cagtagttat ttgccttgat aaaattgaaa
     4261 gcatattcat aacaatatga tataataaat ttgaagtaat attcaaggag aatcaaatga
     4321 tttacgaatt ttgtgctgaa aatgtgactt tacttgaaaa agcgatgcag gctggagctc
     4381 gtcggattga actctgtgat aatctagcag ttggtgggac aacacccagc tatggagtga
     4441 ctaaggcagc ggttgaactg gcagctaact acgatacaac catcatgacc atgattcggc
     4501 cacgtggtgg tgactttgtc tataatgacc tagaaattgc tatcatgcta gaagacattc
     4561 gtttgactgc tcaggctgga agtcaagggg ttgtatttgg agctttaact gctgataaaa
     4621 agttggataa gcctaatctg gaaaagttaa ttgctgcatc aaaaggaatg gaaattgtct
     4681 ttcacatggc ctttgatgaa ctaagtgatg aagatcaacc ggaagctatt gactggctca
     4741 gtcaagccgg tgtcactcgt atcctaactc gtgctggtgt gtctggcgac tccttagaaa
     4801 aacgttttgt tcactatcac agaattttgg agtacgctaa aggtaaaatt gaaattctac
     4861 caggtggggg gattgacctt gacaaccgtc aaacctttat cgaccagttg ggggtaacac
     4921 aattgcatgg tactaaggtt gttttttaaa aaatagaaag gaactgctag ctttgggtag
     4981 cagttttcac ttatgtttga aatttttaaa tcctatcaat ttaatcaaga aaaggttcat
     5041 gattatggtt ttatagaaaa tagcgaagtc tggacatata gttgccagat tttgcaaggt
     5101 gactttgtca tgactgtgtc catcactgct gataatgtga actttcaagt ctttgaccaa
     5161 gagactggtg acctctatcc tcacgttcat atggaaagca tgaggggaag ttttgtcgga
     5221 aatgtccgtg aggcttgtct ggagattctt taccagattc ggaaggcttg ttttgatgtg
     5281 caagatttta tctgtcatca gactaagcgt atcatgactc aagttcagga aaagtatgga
     5341 aaccagttgg agtatctgtg ggaaaaatcg cctgatacag ctgtattgcg ccatgaaggc
     5401 aatcaaaagt ggtatgccgt cttgatgaaa atctcttgga ataagctgga aaagggcaga
     5461 gaaggacaag tggaagcagt caacctcaag catgaccaag tagctaattt gctttcacaa
     5521 aaggggattt atccagcctt ccatatgagc aagcgctact ggattagtgt gtcccttgat
     5581 gatactttat cagatgaaga agtactggaa ttgatagaaa aaagttggaa cttaacctct
     5641 aaaaaatgaa atattttaat aattttcatg aactttcaat tagctaaata ttctttactg
     5701 aagaaatttt cagaaaatat aggatttacc acaccagagg aatgtggtgc catcttcaaa
     5761 tacctgattg agaatgtcaa gacggatcgt cagatcatct attcgcctca ctgtcatgat
     5821 gacctcggaa tggcagtggc aaatagcctt gctgctgtca agaatggtgc aggacgtgtt
     5881 gaagggacta tcaatggtat tagggagcga gctgaaaatg ctgctttgga agaaattgca
     5941 gtggctctca atatttgcca agattactac caagtagaaa ccagtattgt cctaaatgag
     6001 accatcaata cgtcagaaat ggtttctcgc ttctctggta ttccagttcc taaaaacaaa
     6061 gccgtcgttg gtggcaatac cttctcccac gaatctggta ttcaccaaga tggagtcctt
     6121 aaaaatcctc tcacttatga gatcatcaca cctgaattgg ttggtgttaa gattccgctt
     6181 ggaaaattat ctggtcgcca tgcttttgtt gagaaactga gagaattggc cctagatttt
     6241 acagaagagg atatcaaacc actctttgct aagttcaagg cactggccga taagaagtaa
     6301 gaaatcacag atgcagatac tcgagctttg gtagctggaa ccatggttga aaatccagaa
     6361 ggcttccact ttgatgattt acaacttcaa actcatgcag ataatgacat tgaagcgctc
     6421 gttagcctag ccaatatgga tggtgagaaa gtcgaattta atgcgacagg gcaaggttcc
     6481 gttgaagcaa tctttaacgc tatcgataag ttctttaacc aatctgttcg tttggtgtcc
     6541 tacactatca atgcggtaac agatggaatc gatgcccagg atcgggtttt ggtcactgtt
     6601 gaaaacagag atacagaaac catctttaat gcagcagggc ttgattttga tgtgttgaag
     6661 gcttctgcta ttgcctatat aaacgctaat acctttgttc aaaaagagaa tgcaggtgag
     6721 atgggacgca gtgtttctta ccacgatatg cctagtgtgt aaaggagaag gctatggcaa
     6781 agaaaatagt agctctagta ggagacggaa ttggcccaga aatcatggag gctggtttag
     6841 aagttctgga ggctctagct gaaaaaacag gttttgacta tgagattgac agacgaccgt
     6901 tcggaggtgc agatattgat gcagcaggac ctcccttacc tgatgaaacc cttaaggcaa
     6961 gtagggaagc agatgctatc ctactagtag ctatcggtag tcctcagtat gatggagtag
     7021 cggttcgccc tgaacaaggc ctgatggctc tccgtaagga actcaatctt tacgctaata
     7081 ttcgtcctgt aaaaatcttt gacagtctca agtatttgtc accactcaaa ccggaacgaa
     7141 ttgctggtgt agactttgtc gtggtgcgtg aattgacagg cgagatttac tttggagatc
     7201 atattcttga agagcgcaaa gcgcgtgata tcaacgacta tagctatgag gaagtggagc
     7261 ggattattcg caaagccttt gaaattgcaa gaaatcgcag aaaaatcctt attagtatcg
     7321 ataagcaaaa tgttctagcg acctcaaaac tctggcggaa agtagctgag gaagtcgcac
     7381 aggatttctc agatgtaacc ttggaacacc agctggtaga ctcagctgct atgcttatga
     7441 ttaccaatcc tgctaagttt gatgttattg taacggagaa tctttttgga gatattttat
     7501 ctgatgaatc aagcgtctta tctggtacac ttggggttat gccatcagcc agtcattctg
     7561 aaaatggacc aagtctctat gaacctattc acggttcagt acctgatatt gcaggtcaag
     7621 gaattgccaa tcctatttcc atgattttat cagttgtcat gatgttgaga gatagtttcg
     7681 gacgttatga ggatacagag cgtatcaaac gtgctgttga gacaagtctg gcggcaggaa
     7741 ttttaacgag agatatagga ggtcaggctt caacaaagga aatgatggaa gctattattg
     7801 caaggttatg aagttagacg aaaaaattac tctagtcctt ttgatttgga atgtcatcat
     7861 tttcttgatt tatggcattg acaaattcaa ggcaagaaga agagcttggc gcatcccaga
     7921 gaaaatctta ctcattttag cccttacttg tggtggtttt ggggcctggt tagctggaat
     7981 cacttttcac cataagactc gaaaatggta ctttaaaaca gtttggtttc ttgggatggt
     8041 gaccacacta gtagccttat attttatttg gaggtaatgg atggcagggt cttcgaggga
     8101 acacgctgct tgggctctag cggactatgg ttttaaggtc gtgattgcag gatctttcgg
     8161 tgacattcat tacaataatg aactcaataa tggcatgttg ccaatcgttc agcctagaga
     8221 ggttagagag aaactagccc agctaaaacc aaccgaccag gtaactgtgg acttggaaca
     8281 acaaaaaatc atctcaccag ttgaagaatt caccttcgag atagatagca agtggaaaca
     8341 taaactccta aatagtttgg atgatatcgg tattaccttg cagtatgaag agttgattgc
     8401 tgcttatgaa aaacaacgac cagcctactg gcaggattag aaaaaataga aaaggaaata
     8461 tagtaaactg aaataagatg taaacaaatg aattggagct taacatccat ttccagcaat
     8521 tttttagaaa ctacagtgga ctattctgga ttcaacacat tataaaatta tgacaaaaca
     8581 cattcacaag aaggctacga cattttaaaa ggtgagggcg gatgtatcgt ttgccctact
     8641 aaagttggtt acattatcat gaccagcaac aaggcaggac ttgagcgtaa gttcgcagcc
     8701 aaagaacgta agcgtaacaa accaggtgtt gttctctgcg gtagcatgga tgaacttcgc
     8761 gctttagcgc aactcaaccc agaaattgaa gcattctact aaaaacattg gatgaagata
     8821 ttcttcttgg ttgtatcctt ccttggaaac cagaagcctt tgaaaaactc aaagcatacg
     8881 gggatggccg tgaagaactt atgactgatg tacgtggtac tagctgtttt gttatcaagt
     8941 ttggaaaagc aggtgaacaa ttggctgcca agctttggga agaaggtaaa atggtctacg
     9001 cctcatatgc ttcaatgaca aaacgattga aactcgctat gagagcaagg tgtaatggtg
     9061 tctatggtcg ataaggacgg caaactcatc ccagaacaag gaggagcacg ttcaacttca
     9121 ccagcaccag ttgtgatccg taaagggctt gacattgata aaatcatgat gcacctgtca
     9181 gatactttta actcatggga ctaccgtcag gttgagtatt attaagataa aaaaagaagt
     9241 ctagtgttat gagatattaa agcttctaac actggacttt ttgtttagaa tttcttttct
     9301 ttttctatag gatatggtat tctatgtaga aaatatatgt taataagtaa tgccaatat
//
LOCUS       DAVKJD010000065         9314 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKJD010000065 DAVKJD010000000
VERSION     DAVKJD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.065"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..667
                     /locus_tag="VW157_001607"
     CDS             <1..667
                     /locus_tag="VW157_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9552879.1"
                     /translation="NNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIYTSFFTKEKIGAS
                     KNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFKNSQLTVLKSLVK
                     TEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPADKIYINSQLLNYQ
                     PFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVAFFIEHSNVLQDI
                     ISP"
     gene            1323..1493
                     /locus_tag="VW157_001608"
     CDS             1323..1493
                     /locus_tag="VW157_001608"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9552880.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEKAG"
     gene            2105..2389
                     /locus_tag="VW157_001609"
     CDS             2105..2389
                     /locus_tag="VW157_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552881.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            2382..2630
                     /locus_tag="VW157_001610"
     CDS             2382..2630
                     /locus_tag="VW157_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552882.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNHVAKQI"
     gene            2726..2944
                     /locus_tag="VW157_001611"
     CDS             2726..2944
                     /locus_tag="VW157_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552883.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(3312..5636)
                     /gene="pflB"
                     /locus_tag="VW157_001612"
     CDS             complement(3312..5636)
                     /gene="pflB"
                     /locus_tag="VW157_001612"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9552884.1"
                     /translation="MVVKTVVESQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            6031..7092
                     /gene="dinB"
                     /locus_tag="VW157_001613"
     CDS             6031..7092
                     /gene="dinB"
                     /locus_tag="VW157_001613"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9552885.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSIAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
     gene            complement(7239..8084)
                     /locus_tag="VW157_001614"
     CDS             complement(7239..8084)
                     /locus_tag="VW157_001614"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9552886.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            8340..9311
                     /locus_tag="VW157_001615"
     CDS             8340..9311
                     /locus_tag="VW157_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9552887.1"
                     /translation="MLYVGIDVAKNKHDVTALNVRGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALKLLSDPNREQFQHDNRQVDLKILARHIHRLKKKQSDWKVQYTRCLD
                     IIFPELDKIVGKHSEYTYQLLTRYPNPQKRIEAGFDKLIEIKRLTASKIQDILSVAPR
                     SIGTTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILA
                     EIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKACARF
                     SPAFKAYLKTKLEQGKHYNVAIIHLAKKLIRTLFYILKKSCHLTNKK"
BASE COUNT         3037 a         1777 c         1698 g         2802 t
ORIGIN      
        1 aaataatatc gattgtatgg ctataggaca ataccttttt aatccacaca atacccccga
       61 taactttatt ttcctacacg ctggattagg tatttacact tcctttttca caaaagaaaa
      121 aataggagcc tctaaaaatc cttatatcgg agaaattgga cacaccattg tcgaattgaa
      181 tgggcaatat tgtgaatgcg gaaaaaaagg ttgtttacaa acatatattt cggatgcttg
      241 gttaatcaaa cacgcccaat tattatttaa aaattcccaa ctaactgtac taaaaagcct
      301 tgtaaagact gaaaaagaca ttcatttaga caccctttta acggcttata atttaggcga
      361 ctccgcttta cgtcaacaaa ttgataaagg agtcaattta ttagccactt ctattgcaaa
      421 tctcctcctc atcaatcctg ctgataaaat ctatatcaac agtcaattgc ttaattatca
      481 acctttcact catgaagtca gggataaaat ccaagaccag ctccacttcg ttccctttac
      541 tcgtaatata gaaattgaaa ttttacctta caacaaacat cgtggaagta taggagcttg
      601 tgcattagct atcgtcgctt ttttcataga acatagcaat gtattacaag atattatttc
      661 accttaatat attagaaatc tatagacctg tttaaatcaa ctataacctg tagtagatat
      721 ctcgtattta gacaatatga aaacaagacg acttccatat aggaaaccgc cttctctcta
      781 tgttgagtga tttatattaa aataactttt cttctagctg cattttatta ttataaaaac
      841 attcatcata accctgaact taaataacga tttttattca agatacatac tcctagaata
      901 aactttatat gaaattctca tttttgtttt tacaattctc cttagttaaa tcttgtttaa
      961 tatatgtttt acatatagta tttagcgcca catagtactg aactctctcc aaaaacggtt
     1021 attcctcttt gaatagggcg ttatcacaag aaaagcatct ccacgtttca acttcatatg
     1081 gctcaaaaac aatcaattga tgctaaaacc tgtacctaga tgtttcggtt cataaaacca
     1141 tgaaactgta aaagtggatg aaattgatag cgatagtcaa atcaagaggc atcataactc
     1201 taaaaagtca caatatataa gttcatcctc ggaaaaatat cattctaatt gttgaaatgg
     1261 atatataaac aggggtatta acattaaatg ctcatgaaac aacgaataca ggtatcaaaa
     1321 ctatgacaaa acaagtccct aaatttacta aagacactgc tcaactttac acctgtaaat
     1381 ggttgttgta taataaagtt acaaagatgt acgaccacac tgttgtaaat catagtgttc
     1441 gcgaatatat tactgatagc atttctacaa atacaattga aaaggctgga taggctttaa
     1501 acgaaatatg tcagtgtttt ggcattccta gccttcatat catttaaaga attgtataga
     1561 caaaattttt cccaatacag acactcgtaa caactgcttc atttttctac caacatattt
     1621 aggaacagga taagatacaa gaggattaat ccatagatca gttctatacc aatctaagac
     1681 aaataagcta aaaaaacgat tgataataag caaatagatt ccaaattttc tctatctgct
     1741 cattttaata aacaatacta gtgtaactat ccttccagtc agaagcttgt caaatcacac
     1801 cgaaaattct tctaaaattt atctcgttag gcaatcaagc aaaaactcga cgatagtaca
     1861 aacattatca tacaggattg acttcctaaa ttatatactt tagtaaggtt ttcgtataag
     1921 aaaaaagatt cattttacat ttctaaacat tcttttctaa gatgaaaaac agaatttttc
     1981 gattgtgatt taaagcaaca agaagatttt cagtatcatc ctatagatac gagctaatta
     2041 agaaaaacta cattttcgaa tataaactac aataatataa actaaatttt ataggaggaa
     2101 gacaatggat tggtacgatt atatgataca ggcatccaaa caatcacaat tcaacgcaag
     2161 ccattggttt cgctatttgc gaaaagttat ttttgaagac tattcttatt taacaaacca
     2221 agatgtagaa aagttgctag actccaaaga actaacccgt tttcaaaaaa ttagcttgaa
     2281 gtatgccttt caagagcata ctccaactca taaatatgtg atttcattaa ataaacctgc
     2341 taagttaacc aatgttcaaa aattgatgga gaaatacaaa catggataaa atgaaaccgg
     2401 tcttccaagc cctaaataag gaattaattc aggaaaatct gactttaaca attatctgtg
     2461 tcgatggtta tgtcttagaa tatcatggtt tacgtgccac acaagatgtt gatgcttttt
     2521 atgaccaaaa tcagaaaatc aatgaaatta ttgctcgtgt aggaaaacag tttaatctta
     2581 acatccacga agagttatgg ctcaacaatc atgttgcaaa gcaaatatga acaaacaacc
     2641 tcccttaagt ttatgcgaat ccttgtactc atttgagaat ctgaccgttt tagtagtccc
     2701 tattgagtat gtgttaggaa tgaagatgat gagtatcaga gagcaggatt tgcaagatat
     2761 tggtgcaatt attaaataca aaaactttca ttcaccattc gataccttta aatatttaaa
     2821 agacatggga tttgatacta ttgacctctc tgttctatta gaaggtttta gttatgctta
     2881 tggtatggat tggttagaaa aattttttaa agaaaatcaa gataaacttc gcgaatttta
     2941 ttaacagtat caaacaagat gattgtcaat tagccagtcg tcttgttttt tatgatgaaa
     3001 tgaaataaaa tctgaacaaa tcgcttagag aattccagtc aatttctagc gctgtttgag
     3061 acgtttcagt ggactatagt aaaatgaaat aagaatagga caaatcgatc aggacagtca
     3121 aatcgatttc taagaatgtt ttagaaataa aggtgtacta ttctagtttc aatctattat
     3181 atttcagatt cggtccacta ttccttccca aaaacattgc atattgcgcc gaaggaattg
     3241 ctcatactat cccaagcaca aaaccctcag tcgtttgacc gagggttcct ttttattatt
     3301 caagaacttg attagctcaa tgcatccaag gcgtcatcca ttgaaagaac ttcgtggaag
     3361 acacgttgtg tcaattcagt tttttgttct ggagtgaggt atttagtgtt tacacagtat
     3421 ccagagatac gtacgataac gtcttcgcct gacatgattt tttcgtaaac atcgttcaag
     3481 tccataacgt tcaagttaac gtgttgtcca ccgttttcga agtaaccatc aaggattgtt
     3541 accaagttat caacttgttc atcacgagtc ttaccaagag cgcgaggtga tacttgtgta
     3601 gtcaatgaga taccgtcagc tgcataacta aagtcaaggc tagaaagtga gttcaagttt
     3661 tgcaaccaac cacctttagc tttgttagat gggttagcac ctggtgagaa gaattcaagt
     3721 ttagacaagt tcacagaacc atcttcgttg aggtatacac ctttgtgaac tggtgagtta
     3781 ccagtttgtt tagagtaagc aacgttagat gtgattgtca aaagtgatac tgtagcttct
     3841 gcgtctttgt atagtttgtg gctacgtaga cgagttgtgt aagcttcgat caaccattct
     3901 gccaattcgt ttgaacgtgg gtcatcttca ccccagcgtg ggtagtcacc gattgtttcg
     3961 taatcgtaga tgtagccatc ttcgtcacgg attggtttaa ctgtagcgta tttgatagct
     4021 gacaatgtat caacagtgtt agcaaatcca caaataccga atcccatgtt ggcacgttgt
     4081 ttagttggca agaaggccat ttgaacagct tcgtagttgt acctatcagt catgtagtgg
     4141 atgatgttca aggcatctac gtaagtgtca gtcaaccagt caagagattt ttcaaagttc
     4201 gctttaactg attcgaattc aagaacttcg tcacggattg gttcgatatc aaatactttg
     4261 tagtctttgt gaacatcgtc gtaaccacca ttcaaaccag taagaagggc tttaagaacg
     4321 tttacacgag caccgaagta ctggatgttg tggcgttgtt cttcattttc tggatcaagt
     4381 ggagacacac agcatgagat acagctcatt tcaccatatc cgtctttagc cattgttgtt
     4441 acaccttcgt attggataga agagtgtttg tggctcatgt gcatacagta gcgacggaag
     4501 ttgtatggca atttgtcagt ccaaagaact gtcaagtttg gttctggtga gttaccgatg
     4561 ttgtcaagag tgttcaagaa acggtagtcc atcttagtaa cacggtgacg accgtcgtta
     4621 cccataccag ccatagaagt tgtgataaag gttgggtcac ctgagtacaa ttggtcataa
     4681 gcttttgtac gagcaaattt aactgtacga agtttcataa cgaaatcatc aacgaattct
     4741 tggatttctg attcagtaaa tgtaccacga gcaaggtcac gttctgcaaa gatgtccaat
     4801 acgattggta cacgacctag agatgtagca gcaccgttaa tcacacggca gacagccatg
     4861 aaagcaatgt taacccattg gattgcttct ttcacgttca tcgctggttt gcgaacatca
     4921 accccgtaaa ggtcacccag gcgaacaact tgttgcaatg cttggtattg aaggtttact
     4981 tcttcacgaa gacggattgt ttcttcatcg atttctttga ttgcattcca gtcgtttact
     5041 ttttcttgca tcaagtagtc tgcaccgtaa agagcaagac gtgcgtaaac accgatgata
     5101 cgtccgcgtg agtatgcatc tggaagacca gttacagtgt gtgcatgacg agcgcgacga
     5161 atatttgaag tgtaggcacg gaaaataccg tcgttaactg ttgttacata tttagtgaag
     5221 atttcgtgaa cagctgggtc tggttcgtat ccattttctt ttaaagtagt ttcagccata
     5281 cggataccac cttttggcat gaagttcaat ttgaagagtt catcattttg gataccaaag
     5341 ataacttcat tttctttgtc gataaatcca gcagggatat cagcgataga tgttggacga
     5401 gtgtccattg ggaaacgagt ttcttcgtag tgtgctttag tttcttctac aattttcttg
     5461 atgtgaagtg aacgctctgt tggtcctgca aggaagcttt cgtctccatc ataaggtgtg
     5521 tagttagctt gtacaaagcg tgatacactt gctttttctt tccaatctac gcctttgaag
     5581 ccttcccaag ctttgtcaaa aatatcttgt gattcaacaa ctgtcttaac aaccatatta
     5641 atgtcctctt ttttctagta acagtcatct gttacattca taagacaagt ataccataaa
     5701 gtaaccgatt tcaacaagtg caaaatccct atttttacac tttcttttct aaaacagtct
     5761 atattttatt tcaaactgta ttatattttt gaaaaaaata aagtcctttt ttcttttttt
     5821 cagaaaaaag ggtataataa aagaaaataa gcagtaacac tcaatggaaa tcgaaaagca
     5881 aactaggaag ctagccgcag gctgctcaaa gcactgcttt gaggttgtaa atagaactga
     5941 cgaagtcagc tcaaaatact gttttgaggt tgcagatgga agctgacgtg gtttgaagag
     6001 atttttgaaa agtataaaaa ggtgctaggc atgttgattt ttcctttgtt aaatgatttg
     6061 tcaagaaaaa tcatccatat tgacatggat gccttttttg ctgcggtgga aatcaaggat
     6121 aatcctaaac tcagaggaaa acctgtcatt attggaagcg accctcgaca aacaggtggg
     6181 cggggagtcg tttctacctg tagctatgag gctcgagctt ttggtgtcca ttctgccatg
     6241 agttccaagg aagcttatga acgttgtccc caggccgtct ttatctcagg aaattatgag
     6301 aaatacaaag ctgtgggact ccagattcga gctattttta agcgctatac agatttgatt
     6361 gaacccatga gtattgacga agcctatttg gatgtgacag aaaataaact cggtatcaag
     6421 tcagcggtca aaattgctcg cctcattcaa aaagatatct ggcaagaact ccatctaact
     6481 gcttctgccg gtatttccta taacaagttc ttagctaaaa tggcgagtga ttatcaaaaa
     6541 cctcatggtt tgacagtgat tctacctgaa caggctgagg attttctcaa acaaatggat
     6601 atttccaaat ttcatggagt aggaaaaaag acagtagaac gtcttcatca aatgggcgtt
     6661 tttactggtg ctgatttact tgaagttcct gaggtgaccc taatagaccg ttttggtaga
     6721 ctaggctatg atctgtatcg aaaggctcgt ggcattcaca actctccagt caaatccaat
     6781 cgcatccgta aatcaatcgg caaggagaaa acctacggga agattctccg tgctgaggaa
     6841 gatatcaaaa aagagctgac tcttctatca gaaaaagtcg ctctcaatct acatcaacaa
     6901 gaaaaagctg gaaaaattgt cattttgaaa atccgctacg aggacttttc aactcttacc
     6961 aaacgaaaaa gtattgctca aaaaacacaa gatgctagtc agataagcca aatagccctg
     7021 caactctatg aagaattaag tgagaaagaa agaggtgtcc gcctattggg gattaccatg
     7081 actggatttt aaagctcaat gaaaatcaaa gagcaaacta ggaggctagc cgtaggctgc
     7141 tcaaaacact gttttgaggt tgcagataaa gctgacgcgg tttgaagaga ttttctaaga
     7201 gtataaaacc ttgaagagtt gccccttcaa ggtttttctt atacaaataa acggacaaaa
     7261 ctgtaaagta gcaaaacact accaagcacg atacggtatt taccaaaaag ggtgaagtcg
     7321 tgttttttca catagctggt caagaagcga atagccacca tgctgaccgc aaaagctact
     7381 cccatcgcga ccaagagtaa aaacaattgc ccaaagctca agagttctcc ggctttcaca
     7441 aatttgaaaa tctttaaggc actagctcca aacataacgg gaatcccaag atagaaggta
     7501 aattctgtca caactgaacg actggttcca tttaacaaac caccgacaat cgttgcacct
     7561 gaacggctag tccctggtaa aagagcaaga acttggaaga gtccgatata gaaagcggtc
     7621 gtataaggaa gcttgtccaa ctctgttaca cttggctcga tagcacgcgc tttattgcgc
     7681 ttttccaaat atatgaaggc aaccccgtag ataatcaaca tgagagcaac tgaaaccatg
     7741 ttatggaagt gggtatcaaa ccaatcatca aatttaaaga caccaagtaa aggtaaagta
     7801 gcaatcaaga ccttcaacca tagtctccaa gtcttacgaa cttcctgttt gtccttagtc
     7861 ggtttaaaag gattgagctt gttaaaataa atcaccataa ctgctaaaat agcaccaagc
     7921 tgaatcacga cattaaacat ggacataaag gcttcatttt gattttggta ttggataaac
     7981 tcctctgcta aaatcaagtg acctgtactg gaaatcggca accattccgt aattccttca
     8041 acaatcccga agaagataga ttttaaaatt tcaataagat acatatattt ctccttatat
     8101 tatatttaac gcagttagct actgccaaag cccaagtggt atacttggaa taagccactg
     8161 tggattagtt catttcctcc aatcgcttga cgatttccga aagggtctaa caacagcttt
     8221 ttgtttcaat tggtaatgag tttgataacg catagacgtt ttatctcgtc tatggtaaca
     8281 aggcaaaaag ttcgtggata accaagtaga cttgaaatac tatatctgga ggtactccca
     8341 tgctttatgt gggcattgat gtcgctaaaa ataaacacga tgttacagcc ttgaatgttc
     8401 gaggaaaaac tgttcttaaa ccactcactt tttcaaataa taaagctggt tttgaactct
     8461 tagatctgtc tcttcgacag ctcaaccaag actgtctcat cgctcttaaa cttctttctg
     8521 atcccaatcg tgaacaattt caacacgata atcggcaagt agacctaaaa atactggcta
     8581 gacatattca tcgtctcaag aaaaaacagt ctgattggaa agtacaatac actcgttgtc
     8641 ttgatatcat ctttcctgaa ttggataaaa tcgttggaaa gcattcagaa tatacctacc
     8701 aactcttgac gcgctaccct aatcctcaga aaaggattga ggcaggattt gataagctga
     8761 tagaaattaa gcgattgacc gcttctaaaa ttcaggatat cctctcagtc gcacctcgtt
     8821 ctatcggaac aacatctcct gctcgtgaat tcgaaatcat cgaaatcatc aaacattaca
     8881 agaggctcat tgacaaggcg gaaacatgtg tcaatgactt gatggctgag ttcaactcag
     8941 tcatcacgac ggttactggg attgggggtc gtttaggggc ggtcatttta gccgagattc
     9001 gaaatattca tgcctttgat aatcctgctc aattacaagc tttcgctgga ctggattctt
     9061 ctatttatca gtcaggtcag attgatttag ctggaagaat gatcaaacgg ggttcccctc
     9121 atctgcggtg ggcactcata caagctgcca aagcatgcgc tcgcttttca cctgctttta
     9181 aggcctatct taagactaag ttagaacaag gaaaacatta caatgtagcc atcatccacc
     9241 ttgcaaaaaa acttatccga accctgtttt atatcctaaa aaagagctgc catttgacga
     9301 acaaaaagtg atgt
//
LOCUS       DAVKJD010000066         9242 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKJD010000066 DAVKJD010000000
VERSION     DAVKJD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.066"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..253)
                     /locus_tag="VW157_001616"
     CDS             complement(<1..253)
                     /locus_tag="VW157_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl pyrophosphate isomerase"
                     /protein_id="HEU9552888.1"
                     /translation="MLEILKEYLLLEEGDYIFTNNGSPLMITCFNYFLKNRFRKSEIK
                     KDDFVLTAHVFRYSHISLLAELEVPITAIMDRVDYTNETK"
     gene            complement(606..1520)
                     /locus_tag="VW157_001617"
     CDS             complement(606..1520)
                     /locus_tag="VW157_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9552889.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGTNNFSLPNVSPTYGQYYTNQSEVIIGKNDLVKVKNTLQYPYSTSAYVESEFR
                     GVKNGKDVTYRGSANFIKDNVLITAAHNIYKHEFGKDAEELYVTPARTPNSVPFGRIK
                     VKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLTGITVTITGY
                     PSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASNRVVGVHTLGDGAN
                     QINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(1670..1936)
                     /locus_tag="VW157_001618"
                     /pseudo
     CDS             complement(1670..1936)
                     /locus_tag="VW157_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(1961..2272)
                     /locus_tag="VW157_001619"
     CDS             complement(1961..2272)
                     /locus_tag="VW157_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9552890.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(2197..2439)
                     /locus_tag="VW157_001620"
                     /pseudo
     CDS             complement(2197..2439)
                     /locus_tag="VW157_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2440..3072)
                     /locus_tag="VW157_001621"
     CDS             complement(2440..3072)
                     /locus_tag="VW157_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9552891.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQDEF"
     gene            complement(3086..4081)
                     /locus_tag="VW157_001622"
     CDS             complement(3086..4081)
                     /locus_tag="VW157_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9552892.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLYDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHIILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(4078..4776)
                     /locus_tag="VW157_001623"
     CDS             complement(4078..4776)
                     /locus_tag="VW157_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9552893.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAVTILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(4854..5864)
                     /locus_tag="VW157_001624"
     CDS             complement(4854..5864)
                     /locus_tag="VW157_001624"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9552894.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLHTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVLGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(5848..6855)
                     /locus_tag="VW157_001625"
     CDS             complement(5848..6855)
                     /locus_tag="VW157_001625"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9552895.1"
                     /translation="MIAVKTCGKLYLAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLAIYGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(6842..7795)
                     /gene="mvaD"
                     /locus_tag="VW157_001626"
     CDS             complement(6842..7795)
                     /gene="mvaD"
                     /locus_tag="VW157_001626"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9552896.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMAFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(7777..8655)
                     /gene="mvk"
                     /locus_tag="VW157_001627"
     CDS             complement(7777..8655)
                     /gene="mvk"
                     /locus_tag="VW157_001627"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9552897.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(8777..9106)
                     /locus_tag="VW157_001628"
     CDS             complement(8777..9106)
                     /locus_tag="VW157_001628"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9552898.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMIPHDFPLYLTVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         2738 a         2122 c         1631 g         2751 t
ORIGIN      
        1 tctttgtttc attagtataa tctacacgat ccattatcgc tgttataggt acttccaatt
       61 cagcaagcaa acttatatgc gaatatctaa aaacatgagc agtcaataca aaatcatctt
      121 tcttaatttc acttttccta aacctgtttt tcaaaaagta attgaaacaa gttatcatta
      181 agggagaacc attatttgta aatatataat caccttcttc taaaagaaga tattctttta
      241 aaatttctaa cactctatca ctaataccaa ttactctata tgaagctaaa cttttcggac
      301 tatccaaaaa cacttcgtta ttggacgatt acattatagt aaaacgaaat aagaacagga
      361 caaatcgatc aggacagtca aatcaatttc taacaatgtt ttagaagtag aggtgtacta
      421 ttatagtttc aatctactat acaagacgaa attgaagaaa tctttccatt ataaaacgca
      481 tattatcaag gtttttaaac acctaataat atgcgttttt tcctatttta aagactttta
      541 tccacctatt atacaaagtt aagtatccat cacagtttac aattagcctt cattacctaa
      601 cccattcacc atccttcaag agagtaacct ttaagaaccg aataaataaa tggcaaattt
      661 cgttcattta atttaactgc actgttaatt tgattagctc catctcctaa agtatgcact
      721 cctactacgc ggttactagc atcataaact gtagatccac tagacccctc taaagtatca
      781 acttggtagt ccaagaacat gccatcatca cttaaaactt gttttttatc tgtatacatt
      841 tgatgaattt taaaattata tgatggatag cctgtgatag tcacagttat tcctgtcaaa
      901 tttttttgac tagtaggaag acccaaagtc cctaattttg caccaatggg ctcttctaga
      961 attaataaag ccaagtcata ttcccttgca tccttagaat ttaaatttct aaattccttc
     1021 aaataacgaa cttcttttac ttttatccga ccaaacggca cactatttgg agtacgagca
     1081 ggagtaacat atagttcttc tgcatcttta ccaaactcat gtttataaat attatgagca
     1141 gcagtaataa ggacattatc ctttataaaa ttggcactcc ctcgataagt tacatcttta
     1201 ccatttttca caccacgaaa ttctgactct acataagctg atgtagaata aggatattgt
     1261 aaagtatttt ttaccttaac taaatcattt ttaccaataa tgacttctga ctgatttgta
     1321 taatattgac cataagtcgg tgaaacattg ggaagtgaaa aattattagt tcctggacct
     1381 ttttctagtg tctcacttac ctcatattct tcacctgtac tagaaataac actcaaagca
     1441 gatactgtcg ataactggct agccaataaa gtactcgcaa taattgaaat acccaatttt
     1501 ttataaacag ttttcttcat tattgtatcc tcctaatgta attatagcgt actattctaa
     1561 atttcttaat ctactataga atcaagaaat ctaccacctt ctttaaatac cctccattat
     1621 cacataaaca ggtaaacttt tcaattaatg actgcgcttt tcaatcacgc tagaggtact
     1681 tacttgcttc tttgatacta agttcagcca ttctttcctt gtttttctca ataaagcatg
     1741 ttacccaagt gggattcgtt ttggagtagt ctcgcagagt ccagccaatg gctttattga
     1801 taaaaaattc tgtttggttc aagttatgaa gtagaatcat ttccattaat tgagtattgg
     1861 tcttctcttt tcttaacaac tggtggtcaa tagcgacacg tctcagccag atattatctg
     1921 ataggctcca ttttatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcagat
     1981 tgctcaaaac actgttttga ggttgcagat agagctgacg tggtttgaag agattttcga
     2041 agagtattaa gattatttct tctagttcag ggtgttcata caccaaactc cctactactc
     2101 gatctaggat atctaccgtg tcccaccagg attttgtcac gactaactgc tctagcttag
     2161 gcaaatcggt ttcctttaga taagactgca ttgctttcaa atagttagca gccacatatt
     2221 ggtattttct aggctccttt tcccagcaag tgtctgcaaa atcccaatcg ataatctttg
     2281 tttttttcgc ttctagtaaa tatttttata gagtttattt ctttcaggca ccgcaatacc
     2341 tagaaaagaa aattgatggc gcatataggc ttccatggac cttgcttttt tagagtcttt
     2401 tgctgcttct agctcctcaa gtaaatctgc taaactcatc taaaactcat cttgccccac
     2461 caaatggtgc tgaaaggcat agacagccgc ctgggtacga tcgctgactt caagtttggc
     2521 aagaatgttg gacacgtggg tcttgaccgt cttgagagag ataaagaggt catctgcgat
     2581 gcgctgattt tcgtagccct tggcgatgag ttggagaaca tctcgctcac gcgcagtcaa
     2641 ttcttcatga agttccatat gattgcggtg gtattcaacc ttcttgctaa cctcttgctc
     2701 aatggccagc tcgccagcag ctaccttact gacggcatga agcaattcat ctgcactaga
     2761 agtcttgagc atatagcctt tggcaccagc atctaagact ggcatgattt tttcattgtc
     2821 caaataagag gtcacaatca aaatcttggc ttcaggccat tctttaagga ttgctaaggt
     2881 cgcgtcaatc ccattcatct caggcatgac aatatccatg acaatgacat ctggacgcag
     2941 ttccaaggcc aagtcaatcc cttgagaccc gttggacgcc tcacccacaa cttctacatc
     3001 gtcttggagg tcaaagtagc ttttcaaacc caaacggacc atttcatggt catctactag
     3061 taaaattttc atctttactc ctttatcatt ccttatctaa caggggaata cggatatcaa
     3121 ctgccagccc ttgcttggga gctgtcaaga gttgaactgt tccagccata tcttcaaccc
     3181 tctccttgat atttcgcagt ccataactca agtcgtctaa gcttcctaac cggaaaccaa
     3241 tcccattgtc caccaccttc agttgcaatt caacatctgt ctgatagagg tagacatcta
     3301 ggcaagatgc ctgggcatgg cggagggtat tgctaatcaa ctcttgcagg atacggaaga
     3361 tatgctcctc gattttctta ggcaatttcg tcatattctg cttgagacta accctaagat
     3421 cactcttgtc ctcaagctct tttaagagaa tttgaatccc ttctatcaag ctcttctgct
     3481 ccagttcaac tggtcgcaaa tgcaagagca aaacccgcaa atccttctgg gctgtttcta
     3541 aaatagctgt gacactctgc aactgggtct gcatcttttc tctatccaat ttcaaagcct
     3601 gctgactgat acccgataaa ataatgtggg ccgcaaacaa ctcctgactg actgtatcgt
     3661 gcaaatcccg agcaattcgc ttccgttcct tctcgatgat ttcctcttcc tgagcaaggc
     3721 tatgattttc agctttttga agagcctctg tcaaaaggtt aagtttacct gataaggact
     3781 tgaaactggc atccaaatct ggatctacaa cctgaactac ttcttgccct gccaataaac
     3841 gcttgagatt agcctgcatt tttcttagag aaagctcttc gatccctcgc caaaacaggg
     3901 ctaagagaca ggtcatggac atgctgaaaa ccaacaataa aaagacaaat ttttctgttt
     3961 tttcgacatc gtacaaaaag atagaccagt caaaatcaag tatttccagc aagctgtggg
     4021 agaaaaaaaa gacaaatagg aaggaggtga gagcaataat gacataggct tgttttttca
     4081 tcctctaacc acctccacat caccaatcat agtggtcaag aaaatcttga cactcttgtt
     4141 actcttgaga tagtcttttg tttcttgatg atagtgttca ttgcggaggg ctcgcttggg
     4201 ctggttgaaa aaagtcaaat ccccatagag acagttaacg ctgagactga cttccacatc
     4261 tacaggtacg atgattttgg tcgttcctac catctttctg aggataatga cattgtcatg
     4321 attggttaag atgaccctct ccagatgaat agtgtccttg cccatgaagc gaaagagatt
     4381 gatatcatcg aattggcaag tctggtagct tgaaaaatga tgaagatttc caaaccaacg
     4441 atttttctcc ttcttaaccg tcacaacctc ttcaaaaacc aaattggtct gctctttttc
     4501 ctggttcatc atcgggtaaa gaagaaagag gctatagata accgcaacaa aaatagctag
     4561 aatcacaaaa ggattgagca taacgatgaa aaagaagaga atggttaccg ctactaaaag
     4621 aagattattt ccctctttac cagtgtagta gcgaatcaaa agcaaaaaga ggaatagtat
     4681 cagcagaaaa cgcgaaaaat gctctgatac catcaaaatc agagctcctg tcagaagaca
     4741 ggcttcgata aataaaaaga ttttaaattt tctcataggt tcatcctctc ccttctattt
     4801 tatcacaatt caaaaaagtc acctcagtct gaggatggaa aaaaaggcgc tggttacgcc
     4861 tttttcatct gatcatttgc ttcttttaat tttccataaa gaagatagtc tactttttgt
     4921 agatctgcta tggtggcaca gttaagggaa cacataatca agcgtagatc tgctttccag
     4981 ccttggacaa tgccaagcac ttcttcaact gtgtaggttt caaccaattc cagaacggtt
     5041 cgtgacaatc ccacagcctt agcaccaaaa accaagcact taatcatatc cagcggattc
     5101 cgaacccctc cactaaccaa gagttcgacc ttatctttcc attcttgggc attgagaagg
     5161 gcctgcatgg tagactgacc ccattgattg aggtaatcac gctggccact acgacggttt
     5221 tcgatatagg caaagctggt gccaccacga cccgataggt ccactgtacg aacaccgaat
     5281 tcataggctc tttcgattgt cttggcatcc attccaaagc ccacttcctt gaggacaata
     5341 ggaacgggaa tttgcttgct ataatctgct agatgcgatt gccagcttct aaacttcctt
     5401 tctccctcgg gcatgagtaa ttcctgcatg acattgacat gcacttgcaa tagaacagga
     5461 ttcatctctt ctacagtatg aagtcctaac tcgacaggct tgtccaatcc aatattggtt
     5521 ccaaggagga gattgggatg actagacttg acagaaaaag aatcatccgt tggatttttg
     5581 agggctgcgc tataagaacc cgttacaaat aaaataccac aggattccgc cacctgagcc
     5641 agcttttgat tgatttctct tcccttatta cttccaccag tcatggcatt gatataaaaa
     5701 ggaaagtccc actttcgacc agcaaactct gtcgaaagat cgatttcatc cagattgtaa
     5761 agaggcaagg aagaatgaat cagctccacc tcatcaaagc tattatagga acttttctgc
     5821 tcaagggcat agaggatatg ctcgtcctta cgatttgtcg tcatgtccta tcctttcttg
     5881 atataagagc tcaatcccca gatcggccca acgatttttt aaggttttgg ttgattgcgc
     5941 atcaaaactc agggcgatgc cacagtcacc accaccagca ccactactct tggcaacggc
     6001 ctgcaaatct tgactggctt ctttcaactg tctaagcaaa ggcgtgtaaa tatctgtact
     6061 caagccttct aaaagcttgc tggctacttc tacttgctcg ataacttttt cggctttccc
     6121 ctgctccaag gcttcgacca aagaaaccac cgtttctttt gaggaactta aaaaattttg
     6181 attgatattt tgcttgattt gctggaccat gtgactcgat acagccactt ccttggtcca
     6241 tcccactaag aaatcacatt ctaaagttgg tttcacttgt gagataaaaa atccccaatc
     6301 acgctccaga actgtcgcca agttttcttc ttctaaccaa gcagccgcct tctggcgatc
     6361 aaatgactgg tagagaacca aatcctctgc cacaatacag gcaaggtcgc ccatggaacc
     6421 attgtctcct cgcttgagca agacagcgct agtcagcttg aacaagagat tctgatcaac
     6481 cgaaagatta tagagagcca gtaaagcctt gacaaccaag acaacgacgc tgccactaga
     6541 acctagacca aactttttcc cttctcgttc cattttgcca tagattgcta gagaaaaagg
     6601 tcttaaattc tgaccgcgaa cagcgaggaa gtctcccatc aaagcaatcg tttcttgaat
     6661 caagctgtag tcaggattgg gccttaagtc cactgcgaaa tcaaacatat ctgaatagat
     6721 acggtagctg tcagaaaaag caatctcagc cctcatatag atgggaatat cctttatcaa
     6781 agctaactgc cctggctcta aaatagcata ttcacctgcc aaatagagtt ttccgcaagt
     6841 tttaacagca atcatcttga ctcaaatcct ttgtttttga cacaatcaag cgataacgct
     6901 gaccgaaaat ttctgacaaa tgctccaagt ctttctcctg acagaagacc ttaacattgg
     6961 gaccagcatc catggtaaag tagcaggcct ctcctttctc acgaagctga cgaacaaagg
     7021 ccatagcctc ataagaggca tccgtcagat aagaaaaggc tggactagca gtctttgtcg
     7081 tagcatgcat agccagggca tttttctccg ttaattctcc aatcttggca aaatcatttt
     7141 ccttgagata aatcagcata tcctgatagt ccttctcaga ctgacgaacc cagtcgtcga
     7201 aagtcgtcga ggtttccaca caaagtttca tcccgtcacg gctagagatt ggttttttct
     7261 tgtcctctag caccaacata atcatagcta gtttcaagtc tgtctctaca gggtaaattt
     7321 ctccactatc cttatcccag gctcctagtg gtccataaaa actccgagaa gaagagcctg
     7381 aggcaaattt ggcttcctgt gccaactgac ttctatccaa tccaagtttg aaataagcat
     7441 tacaagcctt gaccagggcg gacaaaccac tagaacttga ggacaggccc gctgccgtag
     7501 gcatattgtt ttgagtatcg atacggacaa agccctcacc agctggacga taacggtcaa
     7561 taatcttact catcttggca tgctcgacct cattttgtag ctgaccattg atgtaaaatt
     7621 cgtcagctgt tacattggct ggtaaaggcg acaaggtcgt ctctgtatac atattttcca
     7681 aagttagaga aatactgcta gtagcaggca ccatctcttt ttcttttttc tttccccaat
     7741 atttgataat agcaatattt gcgtaggaac gtactgttac aggctctcta tccatgtctg
     7801 aacagctcct ttctcttcta atctttctgc tagttcttgt gcgtgtgtca aattggttac
     7861 caaggctatg atacaacctc ctagcccacc accgctcatc ttggcaccca gagcaccatg
     7921 gctaagagtc gtttcaacca aaaagtctgc ctcagggcta ctgactccaa tttcttttaa
     7981 atgtaaatgc gcttgactga ggatttgtcc cagtccttca gcatcttttt gtgaaatcgc
     8041 aacttctgct tgctgggtta attctcccaa ggcatgcaaa aacggtaggg catccttgcc
     8101 cttattttga accacttgga tggcttcacg agtatgacca taaacacccg tatcggcaat
     8161 caccaaatag gcggataaat ccatctcaag ttctgtaaat cctacgttct tgataaagcg
     8221 aataggttgg tcactgagac aggtcttagc atccaaacca ctaggattca tatgggcaat
     8281 catttcagct cgattgacca agatttctag tacatcatga ggcagatcag cctgatagta
     8341 gtcaaatacc gcacgaatgg ccgctatgct gatagccgct gacgaaccca tcccccgttt
     8401 ctcagggata gccgagtcaa tctcacaacg aatgcaggct tctgtgatat ccaaatactc
     8461 cagtgaggca taaaccgcca tggacaaggt atcctcctca taaaggcgcc aaggactctc
     8521 tgcaggaact accttacagg tcacctccac ctccaaaaga ggcagggaaa tggcaggata
     8581 accgtaaacg accgcatgtt cccctattaa aattatctta ctatgtgcct gaccgacacc
     8641 aacttttttt gtcatttttt ccttttacta gacgaaaaaa cgtcttattt ttcatacaag
     8701 tattaattct ttcctatcta ttttattata ttttcacaaa aaaagcgatt gtttccattc
     8761 acaatcgctt ctttcattat tgaacccatt cgccattata gttgacagaa tagccatcta
     8821 cggtcgtatt cactgccaag gcacctgagc tataagcgta gtaccatctg ccattgacct
     8881 ggaaccaacc tgtcgtcata gaacgacgaa agaaactcca taccgttaag taaagaggaa
     8941 agtcgtgagg gatcatgcgc cattgacaac ctgttttagt gacgtacaaa gtctcattaa
     9001 caagtactcg tttcggccat ttataggtgc ggtgtttgga gaaatagggt tcaatcttag
     9061 cccattcttg atcgtttaaa tcagtatcat atgctttgcg tgtcataact ctagtttaac
     9121 attttttgtg aatacaagtt ctaaataatc gaccacgaaa atttcttaag tggaaaacgc
     9181 cttatgaagt atgctacggg aaagttatgc acttaatttg acaattcaag atgtaaaaat
     9241 at
//
LOCUS       DAVKJD010000067         9200 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKJD010000067 DAVKJD010000000
VERSION     DAVKJD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.067"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            76..881
                     /locus_tag="VW157_001629"
                     /pseudo
     CDS             76..881
                     /locus_tag="VW157_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            918..1394
                     /locus_tag="VW157_001630"
     CDS             918..1394
                     /locus_tag="VW157_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9552899.1"
                     /translation="MPYFIGGHPGFNCPLLDDEVYEDYYLESEKEETCSVPRLFPETG
                     MLDFQDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVNFAEFP
                     NLIIWSTLNKGPFITFEPWSGLSTFLEEGEHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            1551..1976
                     /gene="lacA"
                     /locus_tag="VW157_001631"
     CDS             1551..1976
                     /gene="lacA"
                     /locus_tag="VW157_001631"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9552900.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2007..2522
                     /gene="lacB"
                     /locus_tag="VW157_001632"
     CDS             2007..2522
                     /gene="lacB"
                     /locus_tag="VW157_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9552901.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            2533..3462
                     /locus_tag="VW157_001633"
     CDS             2533..3462
                     /locus_tag="VW157_001633"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9552902.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQEFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGILNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            3464..4444
                     /gene="lacD"
                     /locus_tag="VW157_001634"
     CDS             3464..4444
                     /gene="lacD"
                     /locus_tag="VW157_001634"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9552903.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            4678..5514
                     /locus_tag="VW157_001635"
     CDS             4678..5514
                     /locus_tag="VW157_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9552904.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            5550..5867
                     /locus_tag="VW157_001636"
     CDS             5550..5867
                     /locus_tag="VW157_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9552905.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5867..7558
                     /locus_tag="VW157_001637"
     CDS             5867..7558
                     /locus_tag="VW157_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9552906.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAKYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            7773..9179
                     /gene="lacG"
                     /locus_tag="VW157_001638"
     CDS             7773..9179
                     /gene="lacG"
                     /locus_tag="VW157_001638"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9552907.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILLVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     SKKSAHWYKKVAETQIID"
BASE COUNT         2897 a         1671 c         1919 g         2713 t
ORIGIN      
        1 gtatcccagc aatgagcgtt ccagtatttg acaatattag acttcctgcg aaacaaaaca
       61 tggtatagta gttccatgaa tgatgaagca agtaaacaac taactgatgc acgatttaag
      121 cgtcttgttg gtgttcagcg taccactttt gaagagatgt tagctgtatt aaaaacagct
      181 tatcaactta aacacgcaaa aggtggacga aaacctaaat caagcctaga agaccttctt
      241 atggccactc ttcaatatgt gcgagaatat cgaacttatg aacaaattgc ggctgatttt
      301 ggtattcacg aaagcaactt actccgtcgg agccaatggg ttgaagtaac tcttgttcaa
      361 agcggtttta cgatttcaag aactcctctc agttctgagg acacggtaat gattgatgcg
      421 acggaagtaa aaatcaatcg ccctaaaaaa agaattagcg aattattatg gtaaaaagaa
      481 atgccacgct atgaaggctc aagcgattgt cacaagtcaa gggagaattg tttctttgga
      541 tatcgctgtg aactatttgt tatgatatga agttgttcaa aatgagtcgc agcaatatcg
      601 gacaagctgg taaaatcttg gctgacagtg gttatcaagg gctcatgaag atatatcctc
      661 aagcacaaac tccacgtaaa tccagcaaac tcaagccgct aacagctgaa gataaagcct
      721 gtaaccatgc gctatccaag gagagaagca aggttgagaa catctttgcc aaagtaaaaa
      781 cgtttaaaat gttttcaaca acctatcgaa atcatcgtaa acgcttcgga ttacgaatga
      841 atttgagtgc tggtattatc aatcatgaac taggattcta gttttgcagg aagtctaata
      901 aagaaactag caaggtcatg ccttacttta ttggtggaca tccaggattt aattgtcctc
      961 tacttgatga tgaagtctat gaagattatt atctagagtc tgagaaagaa gagacttgct
     1021 ctgttccacg tcttttccca gaaacaggta tgttggattt ccaagataga agtccatggc
     1081 tagagggaca aaaagaaata gatcttagtt atgatctttt cagcacagat gcagtgactt
     1141 tggatgaatt gcaatctcga acaattgccc ttcgttctct taaacatgat aagggattga
     1201 aagtgaattt tgcagaattt ccaaacctca tcatctggtc aactttgaac aagggacctt
     1261 tcatcacctt tgaaccatgg tctggcttgt caacattcct tgaagaagga gaacatttag
     1321 aagataagaa gaatgtttgt ctcttagaag ccaatcaggt tgaagaatta gggtttgaga
     1381 tagaagtttt ataaaaaagt aaaaaataaa taacaaaaac aaacacaaac tgttgacttc
     1441 ttcaaacaat agtagtatat tatgtttgtg aggactaatt gactgttttt taaagacagc
     1501 caatacactg ctggaatcca gcatagaaaa aataaaaagg agtatacaat atgtctattg
     1561 ttatcggtgc agatgctgca ggtttgagat tgaaagaagt tgtgaaagac ttcttagaaa
     1621 aagaaaactt ccaccttgtg gatgttacag ctgaaggtca agactttgtt gatgtgactc
     1681 tcgctgttgc tgcagaagta aacaaagaag aacaaaacct tggtatcgtg attgatgctt
     1741 atggagctgg tccatttatg gttgcaacta agatcaaagg aatggttgct gcagaagtat
     1801 ctgacgaacg ttcagcttat atgactcgtg gccacaacaa ctcacgtatg atcactatgg
     1861 gggcacaact tgttggtgat gaattggcta aaaacatcgc taaaggattt gttaatggta
     1921 aatacgacgg tggtcgtcac caaatccggg ttgacatgtt gaacaaaatg ggctaattag
     1981 attatagtaa gaaaggtaag ttaaaaatga gaattgcaat tggatgtgac cacatcgtaa
     2041 ctgatgaaaa aatggcggtt tcagaatttt tgaaatcaaa aggatatgaa gtcattgact
     2101 ttggtacata tgaccataca cggactcact acccaatctt tggtaaaaaa gtaggggaag
     2161 cagtaactag tggtcaagct gatctcgggg tatgtatctg tggtactggt gttggtatca
     2221 acaacgctgt aaataaagtt ccaggtgttc gttctgcctt ggttcgtgat atgacaacag
     2281 ccctttatgc taaagaacaa ttgaacgcca acgttatcgg ttttggtggt aagattactg
     2341 gtgaattgct tatgtgtgat atcatcgaag ctttcattca tgctgaatac aaaccatctg
     2401 aagaaaacaa aaaattgatt gcgaaaattg aacaccttga aagtcacaat gctcaacaaa
     2461 cagacgcaaa cttcttcact gaattccttg aaaaatggga tcgcggagaa taccacgact
     2521 aagaggtaac ctatgatttt aacagttaca atgaacccat ccattgatat ttcctatccc
     2581 ttggatgagt tgaagattga tactgtcaat cgtgtggtgg atgtaaccaa aacggctggt
     2641 ggtaagggac tcaatgttac ccgagtactt tcagaatttg gtgattctgt tcttgctact
     2701 ggtttagtgg gtggcaaact tggtgagttt ttggttgaac atatcgataa tcaagtaaag
     2761 aaagatttct tctcaattca gggagaaact cgtaactgta tcgctattct ccacggagac
     2821 aaccaaacag aagttcttga aaaaggtcct gaagtattgg aacaggaagg tcaagagttt
     2881 ttggaacatt tcaaaaaact cttggagtca gttgaagtag tggccatctc aggtagtctg
     2941 ccagctggcc ttccagttga ttactatgcg agcttggtag aacttgctaa tcaagctggc
     3001 aagcctgtag tcttggactg ctcaggtgca gcacttcagg ctgttcttga atcaccccat
     3061 aaaccaacag tcatcaaacc aaataatgaa gaattgtctc agcttcttgg aagagaagtt
     3121 tctgaggatt tggatgaatt aaaagaagta cttcaagaac ctttgtttgc agggattgaa
     3181 tggattatcg tttcacttgg tgccaacggt acttttgcca aacatggtga cactttctac
     3241 aaggtagata ttcctagaat tcaggtggta aatcctgttg gatctggaga ctctactgtg
     3301 gcaggaattt cttcaggact tcttcacaaa gaatcggatg cagaattact catcaaggca
     3361 aatgtccttg gtatactcaa tgctcaagaa aaaatgactg gtcatgtcaa catggccaac
     3421 tatcaagttc tatatgatca attaatagta aaagaggtat aaaatggctt taacagaaca
     3481 aaaacgtgta cgcttagaaa aactttctga tgaaaatggt atcatctcag ctcttgcatt
     3541 tgaccaacgt ggtgctttga aacgcctcat ggctcaacac caaacagaag aaccaactgt
     3601 ggcccaaatg gaagaactta aagtcttggt agcagatgaa ttgactaaat acgcttcatc
     3661 tatgcttctt gaccctgagt atggacttcc agcaactaaa gctcttgatg aaaaagctgg
     3721 tcttctcctt gcttatgaaa aaacaggtta tgacacaaca agcacaaaac gcttgccaga
     3781 ctgcttggat gtctggtctg caaaacgtat taaagaagaa ggtgcagatg cagttaaatt
     3841 cttgctttac tatgatgtag atagctcaga cgaactcaat caagaaaaac aagcctacat
     3901 cgaacgcatc ggttctgagt gtgtggctga agatatccca ttcttccttg aaatccttgc
     3961 ttacgatgaa aaaattgcgg atgcaggttc tgtagaatac gcgaaagtaa aaccacacaa
     4021 agttatcggc gctatgaaag tcttttcaga cccacgcttt aacattgatg tcttgaaagt
     4081 tgaagttcct gttaacatta aatatgttga aggcttcgct gaaggtgaag tagtttatac
     4141 acgtgaagaa gcagcagcct tcttcaaagc gcaagatgaa gcaacgaact tgccatacat
     4201 ctacttgagt gctggtgtat cagctaaact cttccaagat actcttgtat ttgctcatga
     4261 atcaggtgca aactttaacg gagttctttg tggccgtgct acatgggcag gatcagttga
     4321 agcttacatc aaagatggtg aagcagcagc tcgcgaatgg cttcgcacaa ctggatttga
     4381 aaacattgat gagctcaata aagttcttca aacaacagcg acttcatgga aagaacgtgt
     4441 gtaagaaagt cctcctagtt taggaacatg aatctaaaaa aatttaaaaa aagttgtatg
     4501 taaaggttta caaaataact tacttgtgct atacttaaat cacaagttaa tacaaggtga
     4561 gtgttactaa gtaatattag gcatgatcac aggtgaatta gaaatcagct gattttctag
     4621 ttcatttgtg gtcatttttt gtacttatat acctttaaga tataaaagga ggttgacatg
     4681 tatcgaattc taaatccaat gaatcacaat gtctcgcttg tcagaaatga taagggagaa
     4741 gaggtgattg taattggtaa gggaattgca ttcggaaaga agaaggggga tttgattgct
     4801 gaaaatcagg ttgagaaaat ctttcggatg aagaccgaag agtccagaga aaactttatg
     4861 gctcttctca aagatgttcc gcttgatttt atcacagtga cctatgaaat cattgataag
     4921 ctatcaaaga aatatcatta tccgattcaa gagtatctct atgtaacctt gacagatcat
     4981 atttactgtt cttatcaagc tctaactcaa ggaaggtaca aggatagtaa tctgccagat
     5041 atttccgcta agtatcctgt cgcttttcaa atcgcaaatg aagcttttga aatttaccgt
     5101 cagaagctag cagatcattt tcctgaggac gaaattattc ggattgctta tcatttcatt
     5161 aatgctgaag gtgaaaatga agtggaactt gtggagtcga ttgataagag gaaagaaatt
     5221 ctcaggaatg ttgaagaagt tttaacggac tatgcaattc aacgaactaa aaagaataac
     5281 catttctatg atcgctttat gatccatttg aattatttct tggattattt agacagatct
     5341 agagatgata accaatcact tctggatatg gaagatcata ttaaacaatc ctatccaaaa
     5401 gccttcgaga ttggttccaa gatctatgat gtgattacgc aacatacggg tcttgatttg
     5461 tataaaagtg aacgagttta tctagttcta catatccaac gtttattgtc ataaaaattt
     5521 atttaaaact atataaggag aattctatca tgaatagaga agaagtaaca ttgttaggtt
     5581 ttgaaatcgt agcctatgct ggcgatgctc gttcaaaact attggaagcc ttgaaggctg
     5641 ctgaagctgg tgattttgaa aaagcggacg ctctggtaga ggaagctggt agctgtattg
     5701 cagaggctca ccacgcgcaa acaagtctat tgactaagga agcttctggt gaggacttag
     5761 cgtatagtgt gaccatgatg catggtcaag atcacttgat gacaactatc ttgttaaaag
     5821 acctaatgca ccatttaatt gaactttaca agagaggagt taaataatga acaaactaat
     5881 tgcatttatc gagaaaggaa agcctttctt tgagaaacta tctcgtaata tctatcttcg
     5941 tgctattcgt gatggtttca ttgcaggtat gcctgttatt ctcttctcaa gtatctttat
     6001 cttgattgcc tttgtaccaa actcatgggg ctttaaatgg tctgatgaag ttgtagcctt
     6061 tctgatgaaa ccttatagct attctatggg tattctggct ctcttggtag ctggtacaac
     6121 agctaagtca ttgactgact cagtaaaccg gagcatggaa aaaaccaatc aaatcaacta
     6181 tatgtcaaca ttgttggcag caattgttgg tttgttgatg ttggcagctg atcctatcga
     6241 aagtggtcta gctactggat tcttggggac aaaaggtttg ctttcagcct tccttgctgc
     6301 cttcgttact gtagccatct ataaggtttg tgttaagaac aacgtcacta ttcgtatgcc
     6361 tgacgaagtt ccaccaaata tctcacaagt ctttaaagat gtgattccat tcactctctc
     6421 agttgtttca ctttatgctc ttgatttact agctcgtcat tttgttggtg caagcgtagc
     6481 tgaatcaatc ggtaaattct ttgcaccact cttctctgca gctgatggct atcttggtat
     6541 taccattatc tttggtgcct ttgccttctt ctggtttgtg ggtattcatg gtccatctat
     6601 tgttgaacct gctatcgcag caattaccta tgcaaatgct gaagtcaact tgaaccttct
     6661 ccaacaaggt atgcacgctg acaagattct tacttctggt acacaaatgt ttatcgttac
     6721 catgggtggt actggtgcga cactggttgt tccattcatg ttcatgtggt taacaaaatc
     6781 aaaacgtaac cgtgcaatcg gacgtgcttc agtagttcct accttcttcg gtgtaaatga
     6841 gccaatcttg tttggtgcac ctcttgtttt gaatccaatc ttcttcattc catttatctt
     6901 tgctccaatt gcaaacgtat ggattttcaa attctttatt gaaactcttg gaatgaactc
     6961 attcactgct aatctaccat ggacaactcc agctccacta ggtctagttc ttggaactaa
     7021 cttccaagtg ctatcattca ttcttgctgc ccttctaatc gtggttgacg ttgtcattta
     7081 ctatccattc cttaaggtct atgatgaaca aattcttgaa gaagaacgtt caggtaagtc
     7141 taatgatgaa ttgaaagaaa aagttgctgc aaacttcaac actgcaaaag cggatgctat
     7201 tcttgaaaaa gcaggtgtcg atgcagcaca aaataccatc actgaagaaa caaatgtcct
     7261 cgttctctgt gctggtggag gtacaagtgg actccttgca aatgctttga ataaggcagc
     7321 agcaaaatac aatgtccctg tgaaagcagc agcaggtggc tatggtgctc accgtgaaat
     7381 gttgccagag tttgatcttg ttatccttgc ccctcaagtt gcttcaaact ttgaagatat
     7441 gaaggcagaa acagataagc tcggtattaa acttgctaag acagaaggcg ctcaatacat
     7501 caaattaact cgtgatggaa aaggtgccct agcattcgta caagagcaat tcgattaagg
     7561 ctaggaactg tgaaacagtc tcccatcgtt acggaaatcg ctatggcgaa tttcctaatc
     7621 gccttattaa ttcgtcggta aaaagatatc gtttttacct cctcatgtca caattcagtg
     7681 acttggcaca agaagtgaga tggagaagca tggctcactg actcctctcc tctcactttt
     7741 actttattta aatcaagaaa taggtgaaaa aaatgacaaa aacacttcca aaagacttta
     7801 ttttcggtgg cgcaacagct gcttatcaag cagaaggtgc tacacatact gatggaaaag
     7861 gaccagttgc ttgggataaa tatcttgagg ataactactg gtacactgcc gaaccagcta
     7921 gtgattttta caatcgatat ccagttgacc tcaagctagc agaagagtat ggtgtcaatg
     7981 gtattcgaat ttctattgct tggtcacgta ttttcccgac tggttacggc caagtaaatg
     8041 ctaaaggtgt tgagttttat cataatttat ttgcagagtg tcacaaacgt catgttgagc
     8101 cctttgtaac tcttcatcac tttgacactc cagaagctct ccactcaaat ggagacttct
     8161 taaaccgtga aaatatcgaa cattttgtag actacgctgc cttctgtttt gaagaatttc
     8221 cagaagtaaa ctattggaca acctttaatg aaattggacc aatcggtgat ggtcaatatt
     8281 tggttgggaa attccctcca ggtatccagt acgaccttgc caaagtcttt caatcacacc
     8341 acaatatgat ggtgtctcat gcacgcgcgg taaaattgta caaagagaaa ggctataaag
     8401 gggaaattgg tgttgttcac gccctgccaa ctaaatatcc tctagatcct gaaaatccag
     8461 cagatgttcg tgcagctgag ttggaagata tcatccacaa taaattcatc ttagacgcaa
     8521 cttatctagg tcgctattca gctgaaacca tggaaggtgt caaccatatc ttattagtca
     8581 atggtggtag tttggatctt cgtgaagaag attttacagc attagaagct gcaaaagact
     8641 tgaatgattt cctaggaatc aactactata tgagtgactg gatggaagcc tttgatggag
     8701 aaactgaaat tatccataat ggtaaaggtg aaaaaggaag ctctaagtat caaatcaaag
     8761 gtgttggtcg tcgtgtagct cctgactatg taccacgcac ggattgggat tggattatct
     8821 accctcaagg tttgtatgac caaatcatgc gcgtgaagaa agattatcct aactataaga
     8881 aaatctacat cactgaaaat ggtcttggat ataaagatga gttcgttgat aacactgttt
     8941 acgatgatgg tcgtattgat tacgtgaaac aacacttgga gatcttgtct gatgcgattg
     9001 cagatggagc taatgtaaaa ggttacttca tttggtcatt aatggatgtc ttctcatggt
     9061 caaacggtta tgagaaacgt tatggtctct tctacgtaga ttttgaaact caagaacgtt
     9121 attctaagaa atcagctcac tggtacaaga aagtagcgga aactcagatt atagactagt
     9181 agaattagtc attagatata
//
LOCUS       DAVKJD010000068         8983 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKJD010000068 DAVKJD010000000
VERSION     DAVKJD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.068"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(331..1179)
                     /gene="dprA"
                     /locus_tag="VW157_001639"
     CDS             complement(331..1179)
                     /gene="dprA"
                     /locus_tag="VW157_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9552908.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1275..1964)
                     /locus_tag="VW157_001640"
     CDS             complement(1275..1964)
                     /locus_tag="VW157_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9552909.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1976..2854)
                     /locus_tag="VW157_001641"
     CDS             complement(1976..2854)
                     /locus_tag="VW157_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9552910.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2844..3713)
                     /gene="licA"
                     /locus_tag="VW157_001642"
     CDS             complement(2844..3713)
                     /gene="licA"
                     /locus_tag="VW157_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9552911.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3730..4752)
                     /locus_tag="VW157_001643"
     CDS             complement(3730..4752)
                     /locus_tag="VW157_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9552912.1"
                     /translation="MINQIYQLTKPKFINVKYQEEVIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVVGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4757..5464)
                     /locus_tag="VW157_001644"
     CDS             complement(4757..5464)
                     /locus_tag="VW157_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9552913.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5800..7287
                     /gene="tacF"
                     /locus_tag="VW157_001645"
     CDS             5800..7287
                     /gene="tacF"
                     /locus_tag="VW157_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9552914.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYSYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFMYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7297..8100
                     /locus_tag="VW157_001646"
     CDS             7297..8100
                     /locus_tag="VW157_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9552915.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8102..8911
                     /locus_tag="VW157_001647"
     CDS             8102..8911
                     /locus_tag="VW157_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9552916.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKYDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYITPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2816 a         1820 c         1521 g         2826 t
ORIGIN      
        1 tcactatact ttcccttata ctcaatgaaa atcaaagcgc aaaccaggaa gctagccaca
       61 ggttgctcaa agcactgctt tgaggttgta gataagactg acgaagtcag ctcaaaatac
      121 tgttttgagg ttgcagatgg aagctgacgt ggtttgaaga gattttcgaa gagtattata
      181 tgagaaaaat cctctactct actaatccac ttagaaaaaa cgcatattat caagtgttaa
      241 aaacttgcta atatgcgttt tattatggaa atgttgggtt cattttctct taaaattgat
      301 tttttcttag aattctagct taggtcattt ttaaaattca aattccgcaa gaacatcttg
      361 cccactggtg accaattttg ctccttcttg aatcaaatga tggcaaccgt ctgatagtcc
      421 atctaaaatg ctaccaggaa tagcaaagac atcgcgtcct tcttccattg ctcgctcaca
      481 cgtaatgaga ctacctgaac gcatcttagc ctctgctaca atcacaccac gacaaagtcc
      541 agcaatgatg cgattacggg caggaaaatg aaatttcaga ggttgttcgc caggtccata
      601 ttcacttaaa accaaatggt cattgccgat gtagtcttgc aagcgtttat tggctttagg
      661 ataaaacaca tccagtcctg ttccaatcac tgcaatggtt tttccgccat tctgaagagc
      721 tgccatatga gctgctgtgt caatgccctt ggccagacca ctgacaataa ccagttcatt
      781 ttccaagcct tgaatgactt tttcaactga tttagctccc tgtttgctac aagcacgact
      841 gcccacgacc gctaccttcg ggaatttcag gaggtcaaga tttcccttgt aaaataaaag
      901 tacaggcgca tcatatattt cactcaaatc ccaaggataa cagtcatcta aaatagagaa
      961 agatggaaat ttttgaaact ctttcgacaa atgcgcatcg tctatctgaa aataacgttc
     1021 cataaaaacg gctggattac ggcaacctga tatatctgca atatcaccca acaaaagctc
     1081 ctgatcaaca ttttcaccgt attctagcac tttcaaaatc tgttgattgg tcaaacctga
     1141 tttttttaac ttatagattt catagtttgt gattttcata aataactcca tttctttttc
     1201 tactcatcta tctattcgta aaagttcgta aaaaatcaaa aaacatccga ctattttgtc
     1261 agatgttgga atctttaatt ttcgttttta agaatttctt ctaatttacg atagtcttgg
     1321 acactatcga tctcataaat gctattgcct tctaattctt caacatagac atctagctct
     1381 ttgatattat ccttaaccat attgtcccag tagagatcaa caaattcgcc acttgcataa
     1441 gccttatcga taaagctgac aatcttttct gcagttggag catcccagaa ggatacacca
     1501 ctaaggatgc gacctgcctt gctatcaaca ataatgtctt gaaccttgta gtcatctcca
     1561 tagaccaaga accattcgtt ggtacaatct tcacgataaa cactaaaata agtcgaacga
     1621 gtcaaatcat tgcggaacat atttttaaag agatagttat ctgcatcaat aacatagctg
     1681 ttggccaatt cttcttttac aagatagaga gagtaaaagt tattgtagtc agcgtattta
     1741 tcattgaaaa cgagacgaac accgtatttc tctttcaagt aatcgaattg ttctttaaga
     1801 taaccaacaa tgatgatgat gtcattgatt cctttttctt tgagaaactc aatttggtac
     1861 tcaatcaaag gtttttgatt aacctgaacc aaggctttag gggtattttc agtcatagga
     1921 cgcaagcgag ttcccaatcc cgctgctaag atgatggctt tcacacgaat ctcctttatt
     1981 ctttaataat aatataaact ccagcaatga cgacaagtga cgtcataatt gtcagcatat
     2041 ctagcggtgc acccaagaaa acaactgcaa acaagaccgt ccatactaca tagctcacgt
     2101 tcaagcctgt agccttggct ggttgcaagc gattgatagc gatataataa gccaagtagg
     2161 aaatcatatc aaaggctaca aagacaatca tgagacctag caattgtcca ttggctactg
     2221 caacaaatga ctgatgagag aagagcacaa tcacaagata ggacaagaac gaagtcactt
     2281 gacggattaa gagggcttcg atttcactca gttcactttc catagcaaag gagctaagaa
     2341 cactctcact tccccatgca atagcacaaa ccaaagcaca gagaatccca atgtagaagg
     2401 aattaacctg ttcaacctta taggtctggg caataatccc tccaataatc aagacaatcc
     2461 caaatacagt atttttcgaa atcttgtgct tcaaaaagaa gaaagccaat agaactgaaa
     2521 tcgcagggta aatagccgat acagatgaag ctaaagaact tccgatatac ttaactgcat
     2581 aaagattggc ctgcataccg atagggcctg ctagcaaggc tccgatgata acactgacat
     2641 tgcgaatatt taagaaaatt gagaggcgaa ctttcccttc ttttaccaag agaaaagcta
     2701 gtaagataaa gatgctcaaa aaatcatgag ctgcagccac cacaaagggt gacaaatctg
     2761 taaaaatcga aaagatataa gcactaaccg ttagacccaa gccccagaaa atacctgaga
     2821 gaaggccaaa aggaactccg tttttacttt tcatctgaac ctccataaga agccaaacct
     2881 ttaacagctc tttggtaacg attcacacca tagtcaccaa aatcttcacc ttgctcttcc
     2941 ttatagacag tccatagact ccaaatagta tcttgtaaaa ttttataaat agcaatcttt
     3001 tcatgagaaa ccggtgtttg gtcactctca tagtgagata agaaagtttc ttcctcttgg
     3061 gaagtgaatt cagactctaa aaagagggca gccaaatccc acattggatc attcattgat
     3121 gaatattccc agtcaatcaa ataaagtcgt ccttgaggtg attcgataaa gttttcaggc
     3181 accaaatcga tatgacaaga ttttctgtca acacctaagt cagccagtct tttctctaag
     3241 gagaagactg catttctaac agattcatag ttggcataag gaatttgttc ttcaatcaag
     3301 gattcgtatt ttttgatttc ttcaaaagga gcaaattctc ctcttaattc cttagcagac
     3361 gtatgaatag tttgtaatat tggagtaatt ttgtcgaact tggtcttgat tgacgttgaa
     3421 tcaagcgtaa tcgcagattc gatatactca tttactttga taccagcttc aatatcaaaa
     3481 agataatttt ttacatctaa gcctaaatcc tttagtagtt caagattgta cttttcatct
     3541 tgtcgattga taagcttttc tgtcccttta ccaaagaatt taacaatgta ttgcttattt
     3601 gttgttttgg ccaaatagtt ttgattggtc attccaccca gctgttcaac actgaggact
     3661 tcctcttctt gactaagtaa ggaagaaatt ttttctttaa tgattttctc cacaattaac
     3721 ctccagtact tatacttccc acttaaacac tgttttaaag gctgtgttta aatcggttgc
     3781 aaagacacgg tgaatatctt taatttctct tacaggttct tctagataaa ggatattttt
     3841 aagacgattg gcaaatttct tgacttccat catttggata gcattttcaa aatcaatacg
     3901 accagaacga gatgacccaa ccaaaatcaa gcccttttct aaggcatcgc gagtattgag
     3961 attgacttta tattcgctaa ctcccatcat gagaatcgtt ccctgaggac gaatgtagcg
     4021 aatcaagtca ttaatagctg gtccagtacc atcaccacca caacattcaa aagcatggtc
     4081 aaaggccaac tcttcaggaa tattatccgt aatatagcat tctttggcaa atgagaagag
     4141 ttccaacttt tcccaatgac gaccaacaac cacaatctct gcttctggca aagtatagtt
     4201 gataatattg gcaaccacaa aagctaaact tccatctcca ataacggcga tccgctcccg
     4261 cttgctatga gcaagagtca atagacgatt catagcgtgc atgcccacac tgacaaactc
     4321 tgtaatggct gcaaccgtat cttcaatagc atcataagct accacacgat ctttagggag
     4381 agaaacaaac tctctcataa agccatcaaa tccactagac aagaaatggg tccctgtcat
     4441 gtagttttca tagaattctt catcactctg cataggagac tgattgggaa tcatgacaac
     4501 tttttgacca acctcgtagg ttccggtcgg gtcagaaatg acggttccac atgactcgtg
     4561 aatcattgcc attggaagct ttttattcaa aatcttggga tcacgttttc cctgatagta
     4621 acgctgatcc gcatgacaga cagccatgta gttgggacgg ataaggatat gattctcttg
     4681 gtcaataacc tcttcctgat atttgacatt gataaactta ggcttagtta gttgataaat
     4741 ttgattaatc attttactag tctttctcaa tcatactttt tgcaatcttc aaatctgtta
     4801 cggttgtaat cttcagattt gagtattcac ctttggccaa ggccacatct tttcctttga
     4861 tcacaaagat tttacatgca tctgtcaaga tttccttctc ttcatcagaa agagatccat
     4921 aaaggtccat gaagtccttg caacggaatg tttgaggtgt ttgtccttga taaaggtgag
     4981 cacgatttgg aatatctgta ataaattgac cattggtact ttcaacgata gtatcaaccg
     5041 cttctaccac tgtgtccact gcgtcatgat tttgggcaag ttggatattg tcctgaatca
     5101 tgcgaagtgt aataaatgga cgaacagaat cgtgggtaac aacgatatcc tctggagtaa
     5161 gcggacgata agcatcaatg gcttcaatga ttttcttaat acttgtattg cggtcagcac
     5221 caccctttgt aatgatgata cgttccttat aaagaggaag atatttatct acaagatctt
     5281 ctgcatgaga aacccagtct ccatgaacac caactacaat tttttcaata cttggctcca
     5341 agacaaattt ttcaattgta tgaatcaaaa taggtcgatc acctagctct aaaaattgtt
     5401 ttggcaagtt actgatcccc atgcgtgtgc cagttccacc ggcaagaatt cctgcataaa
     5461 tcatctattt tctcctttgt cttactaaaa aaacttattc tctctattat accacaatct
     5521 agtcctatca taaaagaaat actggtcctc cccttctaga ataggagaat tatgcagttc
     5581 tattattcaa agaaactagt ccatttctag taatgactag gaattctgta gcaattacta
     5641 ataaatggct gcgaaatttt agagtccttc agaataatat actttcctta aagaaaactt
     5701 aaaatttaag atttgaaact tcaaagtgat tgcttggctt actgcccttc atttcttatt
     5761 agctaacttt atgatataat gaaaaacgag gtaaattgaa tgaaaagtat aaaattaaat
     5821 gctctatctt acatgggaat tcgtgtcttg aatattattt ttcccatcct aactggaacc
     5881 tatgtcgcgc gtgtcttgga ccgaactgac tatggttact tcaactcagt cgacactatt
     5941 ttgtcatttt tcttgccctt tgcaacttat ggtgtctata gctacggttt aagggctatc
     6001 agtaatgtca aggataacaa aaaagatctt aacagaacct tttctagtct tttttatttg
     6061 tgcatcgctt gtacgatttt gaccactgct gtctatatcc tagcctatcc tctcttcttt
     6121 actgataatc caatcgtcaa aaaggtctac cttgttatgg ggattcaact cattgcccag
     6181 attttttcaa tcgaatgggt caatgaagct ctggaaaatt acagttttct cttttacaaa
     6241 actgccttca tccgtatcct gatgctggtc tctattttct tatttgttaa aaatgaacac
     6301 gatattgttg tctatacact tgtgatgagt ttatcgacgc tgattaacta cctgattagt
     6361 tatttttgga ttaaaagaga catcaaactt gttaagattc acctaagtga ttttaaacca
     6421 ctctttctcc ctctgacagc catgttagtc tttgccaatg ccaatatgct cttcactttt
     6481 ttagatcgcc tcttcctcgt taaaacaggg attgatgtca acgttagtta ctataccata
     6541 gctcagcgaa ttgtgaccgt tatagctggg gttgtaacag gtgcaattgg agtgagtgtg
     6601 cctcgtctca gttactatct ggggaaagga gacaaagaag cctatgtttc tctggttaat
     6661 agaggtagtc gaatctttaa cttctttatc attccactga gttttggact catggtttta
     6721 ggaccaaatg ccatcctact ttacggtagt gaaaaatata tcggaggcgg catcttgacc
     6781 tctctcttcg cttttcgtac gattatcctg gccttagata ccattcttgg ttcccaaatt
     6841 ctctttacca atggctatga aaaacgtatc acagtttata cagtctttgc tgggctactc
     6901 aatttgggct tgaatagtct cctctttttc aaccgtatcg tggctcccga atactactta
     6961 ctgacaacta tgttatcaga ggcttctcta cttgttttct atatcatttt catccataga
     7021 aaacaactca tccacttggg acatatcttt agctatactg ttcgatactc tctcttttca
     7081 ctttcctttg tagcaattta tttcctgatt aatttcatgt atcctgtgga tatggtcatt
     7141 aatttgccat ttttgattaa tactggtttg attgtcttgc tatcagctat ctcttatatt
     7201 agtctacttg tcttcacaaa agatagcatt ttctatgaat ttttaaacca tgtcctagcc
     7261 ttaaaaaata aatttaaaaa atcataggag tttaaaatga aacaactaac cgttgaagat
     7321 gccaaacaaa ttgaattaga aattttggat tatattgata ctctctgtaa aaagcacaat
     7381 atcaactata ttattaacta cggtactctg attggggcgg ttcgacatga gggctttatc
     7441 ccttgggacg acgatattga tttatccatg cctagagaag actaccaacg atttattaac
     7501 atttttcaaa aggaaaaaag caagtataag ctcctatcct tagaaactga taagaactac
     7561 tttaacaact ttatcaagat aaccgacagt acgactaaaa ttattgatac tcgaaataca
     7621 aaaacctatg agtctggtat ctttatcgat attttcccta tggatcgctt tgatgatcct
     7681 aaggtcattg atacttgtta taaactggaa agcttcaaac tgctgtcttt cagtaaacat
     7741 aaaaatattg tctataagga tagcctttta aaagattgga tacgaacagc cttctggttg
     7801 ctccttcgac cggtttctcc tcgttatttt gcaaataaaa tcgagaaaga aattcaaaaa
     7861 tatagtcgtg aaaatgggca atatatggct tttatccctt caaaatttaa ggaaaaggaa
     7921 gtcttcccaa gtggtacctt tgataaaaca atcgatttac cctttgagaa tttaagcctt
     7981 cctgcacctg aaaaatttga tactattttg acacaatttt atggagatta tatgacccta
     8041 ccaccagaag aaaaacgctt ctacagtcat gaatttcacg cttataaatt ggaggattag
     8101 gatgcaatat ttagaaaaaa aagaaattaa agaaattcaa ctagccctgc tggactatat
     8161 tgatgagact tgtaagaaat atgatattcc ttattttctc agttatggaa ctatgcttgg
     8221 agccatccgc cacaaaggta tgattccttg ggatgatgat attgatattt ccctttatcg
     8281 tgaggattat gagcgtttac tgaagattat tgaagaagaa aatcaccctc gctacaaggt
     8341 tctttcctac gatacatctt cttggtactt ccataatttc gcatcgattt tggacacttc
     8401 tactgttata gaagaccatg ttaagtacaa gcgtcatgat accagccttt tcatcgatgt
     8461 cttcccaatt gatcgattta cagacttgag cattgtcgac aagagctata agtatgtagc
     8521 ccttcgtcaa ctagcttata tcaaaaaatc acgagcagtt cacggtgata gcaaactaaa
     8581 agattttctt agattatgta gctggtacgc tctccggttt gtcaatcctc gctactttta
     8641 caagaaaatt gatcaactag tcaaaaatgc tgtaaccaac actcctcaat atgaaggagg
     8701 agttgggatc ggtaaggaag ggatgaaaga aatcttccca gttgatacct ttaaagaact
     8761 gattttaact gagtttgagg gccgtatgtt gcctgttccc aaaaaatatg accaattttt
     8821 aacccagatg tatggcgatt atataacacc accatcaaaa gaaatgcaag agtggtatag
     8881 tcatagcatt aaagcttatc gcaaaaactg attgaggggg attatacaaa ctactaagat
     8941 agaggttatt caaaaacata attttagtag aaaatgaaat aca
//
LOCUS       DAVKJD010000069         8920 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKJD010000069 DAVKJD010000000
VERSION     DAVKJD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.069"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..212
                     /locus_tag="VW157_001648"
     CDS             <1..212
                     /locus_tag="VW157_001648"
                     /inference="COORDINATES: protein motif:HMM:NF019216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552917.1"
                     /translation="WARQQKVTIDNINRLEKMIEDAVKFDAEDEVAKLYPETNSRVLK
                     LKKAIFKAYLDQTGDFRSSIFENKK"
     gene            256..5469
                     /locus_tag="VW157_001649"
     CDS             256..5469
                     /locus_tag="VW157_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9552918.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRAETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNVDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            5723..6574
                     /gene="ylqF"
                     /locus_tag="VW157_001650"
     CDS             5723..6574
                     /gene="ylqF"
                     /locus_tag="VW157_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9552919.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            6561..7340
                     /locus_tag="VW157_001651"
     CDS             6561..7340
                     /locus_tag="VW157_001651"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9552920.1"
                     /translation="MATIKEIKEFLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            7356..8906
                     /locus_tag="VW157_001652"
     CDS             7356..8906
                     /locus_tag="VW157_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9552921.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         2971 a         1529 c         1856 g         2564 t
ORIGIN      
        1 cttgggctcg acaacagaaa gtgactattg ataatattaa tagacttgaa aaaatgattg
       61 aagatgcagt aaaatttgat gctgaggatg aagttgccaa attatatcct gagacaaata
      121 gtagagttct taaactgaaa aaagcaatct ttaaagctta tcttgaccaa accggtgatt
      181 ttagaagttc aatttttgag aataaaaaat agtgtttact attaggaaat aaaaagtgaa
      241 ggtggaggat tagaattgtc attattaaaa aaagataaat tttccattcg gaaaataaag
      301 ggtattgttg gttcggtatt cttggggagt cttttatttg caccgtcagt tgttggtgca
      361 tcgacttatc attacttaga ttatagtagt ttgacacaaa ctgaacgtga tcaactcaaa
      421 caaggtagac ctgatgaatc caaagagtca tatgctttgg attatgaaaa agacgcacta
      481 ccaaacacag gaagttctca atctattatg actgcattgg gtttgttagc tatcgggagt
      541 cttattgtga ttatcacaaa agataataga aataaaaaaa tagctacatt tttgattgta
      601 ggggcgacag gtttagttac actatcaact gcttcagcac ttaatttgaa tgctaacatc
      661 catgagtctg gacgtgatgg tgtgttgcaa atttctggtt acagatatgt tggttacttg
      721 gagcttgatg ataaaactgt ttcgtcggtt tctccagcat caactgtttc gccagttgag
      781 caacccaaag ttgtaacaga gaaaggcgaa ccggaagttc atgagaagcc tgactatacg
      841 caaccaatag gtgcaaacct tgttgagcct gaagttcatg agaaactagc atacactgaa
      901 ccagtaggga caacaggagt agatgaaaat ggaaacttga ttgaaccacc tgttaacgac
      961 atcccagaat atactgagcc agtaggtaca acaggagtgg atgaaaatgg aaacttgatt
     1021 gagccacctg ttagcgacat tccagagtat actgaaccaa tatctacagt ttcagaagtt
     1081 gcatcagaaa gagaagagtt gccttcttta cataccgata ttcgggcaga gactattcct
     1141 aaaaccacta tagaagaatc tgatcctagc aaatttatag gcgatgattc tgttagacaa
     1201 gttggtgagg atggcgaacg tcaaattgtt actagctatg aagagttaca tggcaaaaaa
     1261 atcagtgacc cagttgaaac agtaactatt ttgaaagaaa tgaagcctaa aattcttgta
     1321 aaaggtacca aagaaaaacc taaagaaaaa acggctcctg ttttgacctt agatagaaca
     1381 aatacaaatg tattaaaccg atccgcaact ctttcttatc atttagttaa tacagatgga
     1441 gtcacaatca ataaaattac tgcgacaatt aaagatggaa atgaaattgt caaaacagta
     1501 gatttgactt cagagcaatt agataagcag gtagaagatt taaaattcta caaggactat
     1561 aagatagaga ccactatgac ctatgaccgt ggcaagggag aagaaactgc aacattagaa
     1621 gagaaaccac ttcgcttgga tttgaaaaaa gttgaaatta aaaatatcgc atctactaat
     1681 ttagtgaagg taaatgacga tggtactgaa acacctagtg attttatgac tgaaaaacct
     1741 tcagatgaag atgtgaagaa aatgtactta aaaattacta gtcgtgataa taaagtaaca
     1801 cgtttagcag ttgataaaat tgaagaagtg actgaagaag gtaagaaact ttataaaatc
     1861 actgccgaag ctcaagattt gattcaacat acagacccaa cgaaggttcg taacaagtat
     1921 gttcattata ttgaaaaacc agttccaaaa gttgatgacg tttattataa ctttaaagag
     1981 ttagtagatg ccatgaatgc tgataaaaat ggtacattta aaatcggagc agatttgaat
     2041 gcaacgaatg taccaacacc taacaaacaa tatgttccag gtacgtttaa gggacatcta
     2101 tcaagtgttg atggtaaaca atatacaatt cataacattg cccgtccctt atttgaccgt
     2161 gttgaaaatg gttctgtgaa aaatattaat ttaggtaatg tggatatcaa tatgccttgg
     2221 gctgacggaa tagcacctgt tgccaatatg gtgaaaaatg caactgttga agatgttaag
     2281 gtaactggta atgtagttgc taataacaat attgcgggta tcgtcaataa aatcgatagt
     2341 ggtggtcagt tgacaaatgt agccttcatc ggtaatctta caggtgttgg tgataaaggt
     2401 caatatatgg ctggcattgc cggtgagatt tggagaggta atcttgcaaa agcatatgtc
     2461 gaggctgata ttgttgctaa cagagcccgt ataggtggtt tagttgctaa aacagataat
     2521 ggtaacgact ctatgggaat tggtaaatac ggttcaattc gtaaatcagt caccaaagga
     2581 accattaaga ctaaagtttt gtttgaaaca ggtggattta tcaatagcaa tcttcctttt
     2641 ggtaaacttg aagataatat ttcaatgatg agagttgaaa atggtgaaga gttctttggt
     2701 tcttccgatt tggactacga tggtggttat ttcacaaatg gctggttaga gcgtaacttt
     2761 gttgtcaaag gtgtcagttc aggtaagcat tcatacaaac gttctcgtga caaaattaaa
     2821 gaaattagtc aagatgaagc taataagaga attgcaaatt ttggacttac tgctgataaa
     2881 tacgaaatca acgaacctgt ggtcaatcgc ttaaatcgtt taaccagaag agaagatgaa
     2941 tataagtcaa ctcaagacta caaatccgaa cgtgatttag cttatcgtaa tattgagaaa
     3001 cttcaaccat tttataataa agaatggatt gtcaaccaag gtaacaaact tgcagaagat
     3061 tcaaacttag ctaagaaaga agtcctttct gttacgggga tgaaggatgg tcaattcgtg
     3121 accgatttat cagatattga taagattatg gttcactatg ctgatggtac taaggaagaa
     3181 atggatgtta ctaagaatac tgattctaaa gtccaacaag tgcgtgaata tagtgtatct
     3241 ggtcttggtg atgttgttta tacaccgaat atggttgtta aaaatcgtga taaattgatt
     3301 gctgatgtta aatctcaatt atcaagtgtg gaattgattt cacaagaagt aagagattta
     3361 atgagtagac gtgataaacc tgcagagaat acggatgaac gtaagaatgg ttatattaaa
     3421 gatctatact tagaagaaag ctttgccgaa gtgaagcaaa atctagataa gttagtcaaa
     3481 tccttggtcg aaaatgaaga ccatcaattg aatggtgatg aagctgcgat aaaatcactt
     3541 ctcaagaaag tggaaaccaa taaagctaaa atcatgatgg ctctgactta tctcaatcgt
     3601 tactatgata tcaaatacgg tgacataagt ataaaaaaca tcatgatgtt caaacctgat
     3661 ttttatggta aaacaccaag tgttattgac agactcatca acatcggttc aagtgaaaag
     3721 aacttgaaag gggatcggac tcaggatgct taccgtgaaa tcatcgcagg taatactggt
     3781 aaaagtaatt tgcgtaactt cttagaatac aacatgcgtc tgtttacaga ggataaagac
     3841 atcaatgatt ggtttatcca ctcagctaag aatgtttatg tttcagaacc taagacaaca
     3901 aatacagagt tgaaagataa acgtcatcgt gtgttcgatg gtttagataa cggtgttcat
     3961 ggtcgtatga ttttaccact tctaacgctt aaagatgccc atatgttctt aatttcaaca
     4021 tataacacga tggcgtacag ttcatttgag aaatatggaa aacatacaga agaagctcgt
     4081 aatgagttca aaactaagat tgatgaagtt gcacatgctc aacaaactta tttagatttc
     4141 tggtccagat tagctctgcc taatgttcgt gatagacttc ttaagagtca aaatatggta
     4201 ccgacaccag tttgggataa ccagacctac aatggttctc ctgtaggtcg tcgaggattt
     4261 gacagtaaag gtaaccctat agctccaatt cgtgaattat atggtccaac atggcgacac
     4321 catgatagag actggcgtat gggcgctatg gcatcaatat tccctaatcc aaataatgat
     4381 gacaaggtct tgtttatggt gacggatatg attagcccat ttggtatttc agcctttact
     4441 cacgaaacaa cacacgtaaa cgatagaatg ctttacttcg gtggtcataa gcatcgtcaa
     4501 ggtacagacg ttgaagccta tgctcaaggt atgttgcaaa cacctgacag ctcaacgact
     4561 aatggtgaat acggcgcact tggaattaac atggcttacc atcgtccaaa tgatggtaac
     4621 caatggtaca acccagatcc tgacaaatta aagacacgtg atgacattga ccgctacatg
     4681 agaaactaca atgaagctat gatgttgctt gatcatgtag aagctgatgc tgtacttcct
     4741 aaaattaaag gagacaatag taagtggttc aagaagattg acaaagaaat gcgctctaag
     4801 attcagtata atgatctttt aggtccaaac caatgggata gtatccgtga tttgaaagat
     4861 gaagaaaagg taatgacttt atctagcgtc aatgatttag tggataataa cttcatgaca
     4921 aaacatggaa atccaggtaa tggacgttat agaccagaag attttacacc aaactcagct
     4981 tacgtaaatg tcaatatgat ggcaggaatt tacggtggga atacgagtca aggtgctcct
     5041 ggttcattat catttaagca caatgctttc cgtatgtggg gctattatgg ttatgaaaat
     5101 ggctttatta gttatgtatc aaataaatac aaagcagaag cagataaaaa caaccacggt
     5161 ttactaagcg ataagctaat aattaataaa gtatcgaaag gaaactttaa tactcttgaa
     5221 gagtggaaga gacattggta cggggaagtt cttgctaaag ctaagaaagg ttttgaggct
     5281 atcgatattg atggcgtcca tatcagtaac tatgatgagt taaggccttt atttgataaa
     5341 gcagttgaag aagatttgaa gaaaccagat gacttctctc acactgttgc tcttaaatct
     5401 aaggtcttca aagcccttct taaaaacaca gacggtttct ttaataaact gtttaaagaa
     5461 gacatttaaa tgtttagaat atagaaggaa cagttttttg ctccctctga aaagtcatca
     5521 tttgatggct tttttctata tagggtactt gaaaggctat catcggacaa aataaagaag
     5581 gcatgatata atataaggta gatttctatg tcataaaaca aaaaactgtt tgaacatcat
     5641 tcatttgaga accctttatg ttcaaacgat agtaaaataa aataggatct ctaaaccctt
     5701 gctacgaaag gaaaaaaact caatggctac tattcaatgg tttcctggtc acatgtctaa
     5761 agcgcgtcga caggtgcagg aaaatttaaa atttgttgat tttgtaacga ttttggtgga
     5821 tgcacgctta cctctatcta gtcaaaatcc tatgttgacc aagattgttg gtgataaacc
     5881 aaaactcttg attttaaata aggcggattt ggctgatcca gcaatgacca aggaatggcg
     5941 tcagtatttt gaatcacaag gaatccaaac tctggctatc aactccaagg agcaagtgac
     6001 tgtaaaagtt gtaacagatg cggccaagaa gctcatggct gataagattg ctcgccagaa
     6061 agaacgtggg attcagattg aaaccttgcg tactatgatt atcgggattc caaacgctgg
     6121 taaatcaact ctgatgaacc gtttggctgg taaaaagatt gctgttgttg gaaacaagcc
     6181 aggggtcaca aaaggtcaac aatggcttaa aaccaataaa gacctggaaa tcttggatac
     6241 accggggatt ctctggccta agtttgagga tgaaactgtt gcacttaagt tggcattgac
     6301 tggagctatc aaggatcagt tgcttcctat ggatgaggtt accatttttg gtatcaatta
     6361 tttcaaagaa cattatccag aaaagctggc tgaacgcttc aaacaaatga aaattgaaga
     6421 agaagcgcct gtgattatta tggatatgac ccgcgccctc ggtttccgtg atgactatga
     6481 ccgtttttac agtctcttcg tgaaggaagt ccgtgatggc aaactcggta actatacctt
     6541 agatacattg gaagacctcg atggcaacga ttaaagaaat caaagaattc cttgtgacag
     6601 tcaaggagtt agaaagccct atttttttag agcttgaaaa ggataatcgc tcaggagttc
     6661 aaaaggaaat cagcaagcgt aaaagagcca ttcaagctga attagatgaa aatttgcgct
     6721 tggaatccat gctttcttat gaaaaagaac tttataagca aggattgacc ttaattgtag
     6781 gtattgatga ggttggtcgt ggtcctcttg ctggtcctgt agtcgctgcg gccgtcattt
     6841 tacctaaaaa ttgtaagatt aaaggtctca acgacagcaa gaaaattcct aaaaagaaac
     6901 atctggagat tttccaagcc gttcaagacc aagccttgtc gattggaatt ggtatcatag
     6961 ataatcaggt catcgaccaa gtcaacatct atgaagcaac caaactagcc atgcaagaag
     7021 caatctccca gctcagccct caaccagagc accttttgat tgatgccatg aaactggact
     7081 tgcccatttc acaaacctcc attatcaaag gagatgccaa ctccctctct atcgcagcag
     7141 catctatagt agccaaggta acacgtgatg aattgatgaa ggaatacgat cagcagttcc
     7201 ctggctatga tttcgctact aatgcaggat atggcacagc taaacatctg gaaggcctca
     7261 caaaactagg agttacccca attcaccgaa ccagctttga acccgttaaa tcactggttt
     7321 taggtaaaaa agaaagttaa ttgaaaggaa ataccatgga ggaacagtcg gaaatagtcc
     7381 gttctaagaa agaattcgcc tttgcatcca gcactatact atcccaagtt ggtcgaggaa
     7441 tcattgtcgg cctcatcgtt ggaattatcg tcggatcctt tcgtttctta attgaaaagg
     7501 gcttccacct gatacaagga gtttatcaag atcaagggta cttagtgcgc aatctttttg
     7561 tactggtttt gttttatata ctcatctgtt ggctcagtgc caaactaaca cggtcagaaa
     7621 aagatattaa aggctcagga attcctcaag tcgaagccga actgaaaggc ctcatgtccc
     7681 tcaactggtg gggcattctt tggaaaaaat atgtgctagg tattcttgct attgccagtg
     7741 gactcatgct gggtcgagag ggacccagca ttcaacttgg agcagttggt ggtaaaggaa
     7801 ttgccaagtg gctcaaatcc agtccagtag aggaacgttc cttgattgcc agtggagctg
     7861 cagcaggttt agccgcagcc tttaatgctc ctattgcagc acttctcttt gttgtagaag
     7921 aagtctatca ccatttttcg cgctttttct gggtctcaac tctagcagcc agcatcgtag
     7981 caaactttgt gtctctactc atgttcggtt tgacaccagt attggatatg ccagataaca
     8041 ttcctcccat gaccctagat cagtattgga tatatctcgt catgggaatt ttccttggat
     8101 tttcaggttt tctctatgag aaagctgtat taaacgttgg aagagtttat gacttgattg
     8161 gtcaaaaaat ccatttggat agggcttatt atcccatctt ggcttttatc cttatcatac
     8221 cagtcggaat cttcttacct caaatcattg gtggcggaaa tcagcttgtc ctttctttaa
     8281 ctgaacaaaa ttttagtttc caagttttat tagcttactt tttaatccgc tttatttgga
     8341 gtatgattag ctatggaagt ggactgccag gaggaatttt cctccccatt ttagctcttg
     8401 gttctttgct tggtgcctta gttggtgtta tctgtgtcaa tcttggactt gtcagtcaag
     8461 agcaattccc tatatttgtc attctaggaa tgagtggcta ttttggagcc atatcaaaag
     8521 ctcccttaac cgctatgatc ctcgtaactg agatggtagg agatattcgc aaccttatgc
     8581 cacttggtct tgtcactctt gtttcttata ttatcatgga tttgctcaaa ggtacgccag
     8641 tctatgaagc catgctggaa aaaatgcttc cagaagaagt atctagcgaa ggagaagtta
     8701 cacttatcga aataccagtt tctgataaaa ttgctgggaa acaagttcat gaactcaact
     8761 taccacacaa cgtcctcatc acaactcaag tccataatgg caagagccaa acggttaacg
     8821 gctcaaccag aatgtatctg ggtgatatga ttcacctggt tattccaaaa agtgaaattg
     8881 gaaaagtcaa agatttgttg ttgtagtatg atcatttaca
//
LOCUS       DAVKJD010000070         8917 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKJD010000070 DAVKJD010000000
VERSION     DAVKJD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.070"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..415)
                     /locus_tag="VW157_001653"
     CDS             complement(<1..415)
                     /locus_tag="VW157_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU9552922.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLRDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVD"
     gene            537..1283
                     /locus_tag="VW157_001654"
     CDS             537..1283
                     /locus_tag="VW157_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9552923.1"
                     /translation="MKRLEQIIKLVSEHEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1296..2276
                     /locus_tag="VW157_001655"
     CDS             1296..2276
                     /locus_tag="VW157_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9552924.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNVGIVKIEIENFDTNMFKLENYVKEKYSLESLEIIPNEFDDTPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            2471..2791
                     /locus_tag="VW157_001656"
     CDS             2471..2791
                     /locus_tag="VW157_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9552925.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            2837..3145
                     /locus_tag="VW157_001657"
     CDS             2837..3145
                     /locus_tag="VW157_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9552926.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3162..4460
                     /locus_tag="VW157_001658"
     CDS             3162..4460
                     /locus_tag="VW157_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9552927.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            4611..7049
                     /locus_tag="VW157_001659"
     CDS             4611..7049
                     /locus_tag="VW157_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9552928.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7068..7736
                     /locus_tag="VW157_001660"
     CDS             7068..7736
                     /locus_tag="VW157_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9552929.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            7754..8842
                     /locus_tag="VW157_001661"
     CDS             7754..8842
                     /locus_tag="VW157_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9552930.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
BASE COUNT         2943 a         1253 c         1810 g         2911 t
ORIGIN      
        1 aatccacata ttgaattaaa tcaataaatt tttcatgatc aacaaaggca gtagtttcaa
       61 tggcagtgtg tatgtgatgt tctttagctg attttaagat ggctttagca aattcaaact
      121 gagcaaatat ttcacctcct gataaagtta aacctccacc ggattcttcg taaaattctt
      181 tgtctcttaa tacctctgta ataatttctt ctacagtctt ttcttcaccg actaaggtga
      241 atttctctcg ttgagcatct ttcatttttt caggtttcat tctttgagat tcaggattag
      301 aacaccatgg acagcgcaga ggacatcctt ttaaaaaaac agttgtacga atacccggac
      361 cgtcatgaat tgaaaagtgt tgaatattaa aaataattcc tttagatatt tccataagag
      421 ttcctcctct tcataagtct atcctatcac aaaaacgaaa gaaaaacaat tacgaacgaa
      481 agtttaattt gttgtttttt gctttttatt ttggtagaat aaaacggagg tgtaaaatga
      541 aaagattgga acaaattatt aaattagtat cagaacatga aaagatcgac gttaatacat
      601 tatcggaaaa attaaatgta tcgaaagtaa cgattagaaa agatttagat aaattagagt
      661 caaaaggttt attacacaga gagcatggat atgctgtatt aaatagtgga gatgacttaa
      721 atgtacgttt gtcaattaac tatgaaatta agagaaaaat tgttcaggaa gcagtaaaat
      781 tggtgtcaga taatgaaaca ataatgatag aatctggatc gacctgtgct ttacttgctg
      841 aggaaatttg caagcaaaaa agaaatgtta cgattgtaac aaattcattt tttatagcaa
      901 attttgtgag agcttatgat tcatgtcgta ttattgttct tggtggagag tttcagaagg
      961 attcacaggt tactgtggga cctttattaa aagaaatgat acagactttt catgtgcatc
     1021 aagcttttgt tgggacagat ggttacgata aagagatggg ctttacagga aaagatttaa
     1081 tgcgcagtga ggtagttcaa tatatttcag cagcgtcgga taaagtcatt gtactaactg
     1141 actcaagtaa atttgataaa agaggtacag taagaagatt tgctttaagt caagtctatg
     1201 aagtaataac agacgaaaaa ctttctaaac aaaatatagc tacattagaa aatgctggga
     1261 taatggttaa ggtagtttcg taagaggtta agtgtatgaa tcaagatagg aataaactgc
     1321 tttctaaaat tgcttatctg tattatattg aaaacttaaa tcagtcacaa atagcagcaa
     1381 aattaggaat ttatagaacc tctattagta gaatgttaac agaagcaagg aatgtaggaa
     1441 ttgttaaaat tgaaatagag aattttgata ccaatatgtt taagttggaa aattatgtaa
     1501 aagaaaaata cagtttggaa agtttagaaa ttattccaaa tgaatttgat gatactccaa
     1561 caattttatc tgaaagaatt tctcaagttg cagcaggcgt ccttaggaat ctaattgatg
     1621 ataatatgaa aattggcttt tcttggggga aaagtttaag taatttagta gatttaattc
     1681 acagtaaaag tgtccgaaat gttcacttct atcctctagc aggtggtcct agtcacatac
     1741 acgctaaata ccatgtgaat acactgattt atgaaatgtc tagaaaattt catggagagt
     1801 gtacatttat gaatgcaacg attgtgcaag aaaataaatt gttagcagat ggtattttgc
     1861 aatcaagata ttttgaaaat ttgaaaaata gttggaaaga tttagatata gctgtagtcg
     1921 gaattggtga ttttagcaat aaagggaaac atcaatggtt agacatgctt acagaggatg
     1981 actttaaaga attgaccaaa gttaaaactg taggagaaat ttgttgtcga ttttttgatt
     2041 caaaaggtaa agaagtctat gaaaacttac aggaaagaac gatagccatc tctttggaag
     2101 atttaaaaaa tattcctcaa agtttagctg ttgcttacgg tgatacgaaa gtatcttcga
     2161 ttctttctgt cttgcgtgct aatttagtaa atcatttgat tacagacaaa aatacaattt
     2221 taaaagtttt ggaagaagat ggggatttga cttttagaga gattctaggt gagtgaaaat
     2281 gatagactga ttcagtttat cgtttttctt tttagttgat tgcacatttg tgcttatata
     2341 aacaaaaata gtttatctgt tgtttttgga ttgacaactt tattatgtag ttgtattcta
     2401 tagttacaaa agaaaatttt aaaatttcaa atgaaaaaag ctttttacat agtgaaatga
     2461 ggaggaattt atggaaatta ttgttccaga tcaaattatc atgggtttaa ttttatatgc
     2521 tggtgatgcg aaacaacata tttataaagc gttagattac ataaaaaatg gtacatgtga
     2581 acgatgtgaa gaagaaatcc agttagctga tgcagcctta ttagaagctc ataatctaca
     2641 aacaaaattc ttggcacagg aagcgtctgg tacaaagaca gaaattacag ctctctttgt
     2701 tcattcacaa gatcatctca tgactagtat gacggagatt aatttaatca aagaaattat
     2761 tagtttgaga aaagaacttc ataaaaaata atactagagt attatcattg ttattaacat
     2821 agaggaggaa aacataatgg tgaagattgg tttgttttgt gcagcaggtt tttctactgg
     2881 tatgcttgta aataatatga aaattgcagc gcaagctagt ggagttgagg cagaaataga
     2941 ggcgttttct cagtctaaat tagcggatta tgcgccaaat atagatgttg cactattggg
     3001 tccacaagtt gcttatacat tagataaatc aaaagaaatt tgtgataagt gtgatgttcc
     3061 gatagctgtt attccgatga tggactatgg tatgttagat gggaaaaaag tattagattt
     3121 ggccctatct ttgattagtg ggtaagaaaa ggagatttat tatggcaaag atggatgttc
     3181 agaaaatcat tgcaccgatg atgaagtttg tgaatatgcg tggcattata gctctaaaag
     3241 atgggatgtt agcaattttg ccattgacag tagttggtag tttgttcttg attatgggac
     3301 aattgccgtt cgaaggatta aataagagca ttgctagtgt ttttggagct aattggacag
     3361 agccgtttat gcaagtatat tcaggaactt ttgctattat gggtctaatt tcttgttttt
     3421 caattgccta ttcttatgct aagaatagcg gagtagaggc tttaccagct ggagttctat
     3481 ctgtatctgc attctttatt ttgctaagat catcttatat ccctaaacaa ggtgaggcga
     3541 ttggggacgc tattagtaaa gtttggtttg gaggccaagg aattatcggt gctatcatta
     3601 taggtttggt agtaggaagt atttatacct tctttataaa gagaaaaatt gttattaaga
     3661 tgccagaaca agttccacaa gctattgcca aacagtttga agcaatgatt ccagcatttg
     3721 taattttctt atcttctatg attgtatata ttttagcgaa gtcattgact aatggcggaa
     3781 cattcataga aatgatttat tctgctattc aagttccgtt gcaaggttta actggatctt
     3841 tgtatggtgc tattggaatt gcattcttta tatcattttt gtggtggttt ggtgttcatg
     3901 ggcaatcggt agtaaatgga gtagtgacag ctctgctttt atctaatctt gatgctaata
     3961 aagctatgtt agcctctggt aatctatcat tagaaaatgg tgcacatatt gttactcaac
     4021 aatttttaga ttcattttta attctatcag gttcagggat tacgtttggt cttgtagttg
     4081 ccatgctttt tgcagcaaaa tcaaaacaat accaagcctt aggaaaagtt gcagcttttc
     4141 cagcaatatt taacgtaaat gagccagttg tatttggatt tccgattgtc atgaatccag
     4201 ttatgtttgt acctttcatt cttgttcctg tacttgcagc tgtgatagta tatggagcta
     4261 ttgcaacagg tttcatgcag ccattctcag gggtaacatt gccttggagt acaccagcta
     4321 ttctatcagg atttttggtg ggtggatggc aaggtgttgt gactcagtta ttagtattgg
     4381 tgatatctac agtggtttac tttccattct ttaagataca agatcgttta gcttaccaaa
     4441 atgaaatcaa acaatcttag aggtatttgt gtgttactgt taaactcaca catttgtgct
     4501 aaaaattaga gagttaaaat ttttctagtt aaaagcttga aaatttctat aaaaatcggt
     4561 attatatttt cgaaagaaat aaaaatattt tcgaaagaaa ggtgcttacg atggtaaata
     4621 cagaagtagc aagaacaaca atcaagacag aatattttgg cagccttact gaaaggatga
     4681 acaaatatcg agaagatgtt ttaaataaaa aaccttatat tgatgctgag agagcagttc
     4741 tagcaacacg cgcctatgaa cgatacaagg aacaacctaa tgtcctaaaa cgtgcatata
     4801 tgctgaaaga aattttggaa aatatgtcta tctatattga agaagaatct atgattgcgg
     4861 gaaatcaagc ttcttccaat aaagatgctc ctatttttcc ggaatatacg ctagaatttg
     4921 ttctcaatga gttggatctt tttgaaaagc gtgatggaga tgttttctat attacagaag
     4981 aaacaaaaga acaacttaga agtattgctc cgttttggga aaataataat ttacgtgcta
     5041 gagctggtgc cttattacct gaagaagtgt ctgtttatat ggaaacagga ttcttcggta
     5101 tggaaggtaa gatgaattct ggagatgctc acttagcagt taactatcag aaacttttgc
     5161 aatttggttt aagaggtttt gaagagcggg ctcgtaaagc aaaagtagct ctagatttaa
     5221 cagatccagc aagtattgat aaatatcatt tttacgactc tatatttatc gtaatcgatg
     5281 ctattaaagt atatgcaaag cgctttgttg ctcttgctaa aagtttagcc gaaaatgcaa
     5341 atcctaaacg taagaaagaa ttacttgaga ttgcagatat ttgctctaga gtcccatatg
     5401 aaccggcaac tacttttgca gaagctattc aatcagtttg gtttattcaa tgtattttac
     5461 aaattgaatc taatggccac tctctttcat atggccgttt tgatcaatat atgtatccat
     5521 atatgaaggc tgatttagaa agtggtaaag aaacagaaga tagcattgtt gaacgtctga
     5581 caaatctttg gattaagaca attacaatca ataaggttcg cagtcaatca catacatttt
     5641 cttcagcagg aagtccttta tatcaaaatg ttacaattgg tggacagact cgagataaga
     5701 aggatgctgt taacccatta tcttatttgg tattaaaatc agttgcacaa acccatctac
     5761 cgcaacctaa tctaactgta cgttaccatg caggtttaga tgctcgtttc atgaatgagt
     5821 gtattgaagt gatgaaactt ggttttggta tgcctgcatt taataatgat gagattatta
     5881 ttccttcttt tattgcaaaa ggagtattgg aagatgatgc ttatgattac agtgccattg
     5941 gatgtgttga aacggcagtt ccagggaaat ggggctatcg ttgcacaggt atgagttata
     6001 tgaacttccc taaggttcta cttatcacga tgaatgatgg aattgatccg gcttcgggta
     6061 aacggtttgc accaagcttt ggtcatttta aggatatgaa gaacttttct gaattagaaa
     6121 atgcttggga taaaacacta agatatttga cacgaatgag tgttattgtt gaaaattcta
     6181 ttgatttatc attggaacga gaagttcctg atattctatg ttcagcattg actgatgatt
     6241 gtattggtcg tggaaaacac cttaaagaag gtggagcagt atatgattat atatcaggat
     6301 tgcaagttgg aattgcaaat ttgtcggatt cattagctgc aattaaaaaa ttggtgtttg
     6361 aggaagaacg tataagccca agtcagcttt ggcatgcact ggaaacagat tatgccggag
     6421 aagaaggtaa ggtcattcaa gaaatgttga ttcatgatgc acctaagtat ggtaatgatg
     6481 atgattatgc tgacaaattg gttactgctg cttatgacat ttatgttgat gaaattgcta
     6541 aatatcctaa tacacgttat ggaagagggc ctattggagg aattcgttat tcaggaacat
     6601 cttctatatc agccaacgta gggcagggac gtggaacatt agcaactcca gatggacgca
     6661 acgcgggtac accgttagca gagggttgtt caccatcaca taatatggat caacacggcc
     6721 ctacatctgt tttaaaatct gtttcaaaat taccaacaga tgaaatcgta ggtggggttc
     6781 tcttaaatca gaaagtaaat cctcaaacgt tagccaaaga agaagataaa ttaaaactaa
     6841 ttgctttgtt acgaacattc tttaatcgtt tacatgggta ccatattcaa tacaatgttg
     6901 tttccagaga gacgttgatt gacgctcaga aacatcctga aaaacacaga gacttaattg
     6961 ttcgtgttgc aggatactct gcattcttca atgttctttc taaggcaacc caagatgaca
     7021 ttataggacg tactgagcat actttgtaaa ataaagaggt tctttttatg gaatttatgc
     7081 ttgacacatt aaatttagat gagattaaaa agtggtctga aattttgccg ctagctgggg
     7141 taacttcaaa tcccactatt gcaaaaagag agggttctat taattttttt gaacgaatca
     7201 aagatgtaag agaattgatt ggctctacac cctctattca tgttcaggtg atttctcaag
     7261 attttgaagg catcttaaag gatgctcatg aaattcgaag acaagcagga gatgatatat
     7321 ttatcaaagt acctgttact ccagctggat tacgtgcaat aaaggtgcta aaaaaagagg
     7381 gctaccatat cactgcaaca gctatttata cagttattca gggattatta gctatcgaag
     7441 caggagcgga ttacctagct ccatattata atagaatgga aaatctgaac attgattcaa
     7501 attctgtcat tcgtcaatta gctcttgcta ttgatagaca gaactctcct agtaagattt
     7561 tagctgcatc ctttaaaaat gtagcacaag taaataatgc tttagctgca ggtgcgcatg
     7621 ctgttacagc aggagcagat gtttttgaat cagctttcgc catgccatct atccaaaagg
     7681 cggttgatga tttttctgac gattggtttg ttactcaaaa tagtcgttcc atttagatag
     7741 agaggaaata catatgagaa tttttgctag tccttctaga tatattcagg gggaaaatgc
     7801 cttgtttgaa aatgccaaat caattttgga tttgggaaat taccctattc tattatgcga
     7861 tcagttggtt tatgatattg ttggaaaacg atttgaagat tatctacata ggtatggttt
     7921 ccatattgtt ctggcgctat ttaatggtga agcttctgac aatgaaatca atcgagttgt
     7981 tgccttggct gagaaagaaa attgtgatag tattatcggt cttggtgggg gaaagacgat
     8041 tgatagcgca aaagctattg cagatttgat tgaaaagcct gttattattg ctccaacaat
     8101 tgcatcgacc gacgcacctg tatctgcttt atctgttatt tatacagatg aaggtgcatt
     8161 tgatcattat ctattttatt ctaaaaatcc agatttagtt ttggttgata caaaagttat
     8221 ttcacaagcc cctaagcgtc tattagcgtc tggtattgca gatggtttag caacttgggt
     8281 tgaggcgcgt gcggttatgc aggcaaatgg aaaaactatg ttgggacaac agcaaacatt
     8341 ggctggagtt gcaattgcga agaaatgtga agaaacgctg tttgcagatg gtttacaggc
     8401 tatggcagct tgtgaagcta aagtggtgac accagcatta gaaaatattg ttgaagctaa
     8461 tactttattg agtggtctag gttttgaaag tggaggatta gctgcggcgc atgcaattca
     8521 taatggtttt actgcattga caggtgacat tcatcattta acacatggtg aaaaagtagc
     8581 ttatggaact ttagtacaac tattattgga aaatagacct aaagaagaac ttgataagta
     8641 tattgagttt tacaaaaaaa ttggtatgcc aacaactcta aaagaaatgc atttggatca
     8701 agttggatat gatgatttaa taaaagttgg taaacaagca actatggagg gtgagacaat
     8761 tcatcagatg ccgtttaaga tttcgccttc agatgttgct caagctatta tcgctgtaga
     8821 tgcctatgta aattcaaaat aaacaataag gactactgtt ttccaaatgg tagtctttta
     8881 ttgatccctg tattgaattc tatagaagat tgaaata
//
LOCUS       DAVKJD010000071         8846 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKJD010000071 DAVKJD010000000
VERSION     DAVKJD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.071"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..146
                     /locus_tag="VW157_001662"
     CDS             <1..146
                     /locus_tag="VW157_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9552931.1"
                     /translation="IKARNIKTSSDIELESDVDGDKSDNLPVDIKILGQHIEVYSVPK
                     KQS"
     gene            346..3621
                     /gene="rexB"
                     /locus_tag="VW157_001663"
     CDS             346..3621
                     /gene="rexB"
                     /locus_tag="VW157_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9552932.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMNFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGAEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPVIE
                     TIKEEANFGGKEQAFIQLDNGRSVFVRGKVDRIDRLKADGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSHLGEFYNKNKANQLTDEEFQLLLDYNAHLYKKAAEKILEGQFAINPYT
                     ENGRSIAPCVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3618..7268
                     /gene="addA"
                     /locus_tag="VW157_001664"
     CDS             3618..7268
                     /gene="addA"
                     /locus_tag="VW157_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9552933.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTTEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILNQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SDSFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7528..8706
                     /locus_tag="VW157_001665"
     CDS             7528..8706
                     /locus_tag="VW157_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552934.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2717 a         1868 c         1892 g         2369 t
ORIGIN      
        1 atataaaggc acgtaatatt aaaacttctt ctgatatcga attagagtcc gatgttgatg
       61 gcgataaatc agacaatcta cccgtcgaca ttaagatact gggacaacac attgaagtgt
      121 attccgtacc taagaaacaa tcataaaact caaagccttg ttaccgaggc ttttttatta
      181 tgtgattttc agtagccaga cttattctat ctaatacaag taaagctaag tatttaagat
      241 ttagcagatg atgaagaaaa gaaaggatgg cctaaatatt ttaacagatt tacgaggcta
      301 atcatggtat aatatagggt gatgaaaaat tccaacgagg ctgagatgaa attactttat
      361 actgatattc gaacttcttt gacagaaatt ctaacaagag aggcagaaga gctagttgca
      421 gctggcaagc gggtcttcta cattgccccc aactctcttt cttttgaaaa ggaacgcgcc
      481 gtgctggaat gcttgtccca gcaggcttct ttttcgatta ccgtcacgcg ctttgctcaa
      541 atggctcgtt atctggtctt gaatgattta ccagctaaaa ctactcttga tgatatcggt
      601 cttgggttgg ccttttacaa atgccttgcc gaactcgatc ccaaggactt gcgtgtttat
      661 ggcgctatta agcaggatcc tcaattgatc cagcagttaa ttgagcttta ccatgagatg
      721 accaaatctc agatgaattt tttggacttg gagaatttaa cagatgagga taagagggcg
      781 gatttactct tgatttttga gaaagtaaca gcctatctta atcaagggca gttagcccag
      841 ggaagtcagt tgtcccattt gattgaggct attgagaatg acaaggtaag tagtgatttt
      901 aatcaaatcg ccttggtcat tgacggcttt actcgttttt ctgctgagga agagcgggtt
      961 gtggacttac ttcacggcaa aggtgctgag attgttatcg gggcttatgc tagtaagaaa
     1021 gcctatacca gtccttttag cgagggcaat ctctaccaag ccagcgtaaa atttctccat
     1081 catctggctt ctaaatacca aacgcccgct caggactgtt ctcaaactca tgagaagatg
     1141 gatagttttg acaaggcctc tcgtttgttg gagtcttctt atgacttttc agaactcgct
     1201 ttggatgtcg atgagaaaga ccgtgaaaat ttacaaatct ggtcttgttt gacgcaaaag
     1261 gaggagttgg agctagtagc ccgtagcatt cgtcagaaat tacatgagaa ctcagacctg
     1321 agctacaagc attttcgtat tctcttgggg gatgtagctt cttaccagtt atctctcaaa
     1381 accatttttg accagtatca gattcctttt tatcttggta gaagcgaagc catggctcat
     1441 catcccttga ctcagtttgt cgagtctatt ttagctttaa aacgttaccg ttttcgtcag
     1501 gaggatttga ttaatcttct tagaactgac ttgtatactg acctcagtca gtctgatatt
     1561 gatgcttttg aacaatatat ccgctatctt ggtattaatg gcttgccagc ctttcagcaa
     1621 accttcacca aatcccacca tggaaaattt aatcttgagc gtttgaatgt cctccgcctg
     1681 agaattttag cacctcttga aaccctcttt gccagccgaa aacaaaaggc tgaaaacctc
     1741 ctacaaaaat ggagtgtctt tctaaaagaa ggagctgtga ccaagcagtt acaagatttg
     1801 acaaccactt tggaagctgt agaacaggaa agacaagccg aagtttggaa ggctttctgc
     1861 catgttttag aacaatttgc gactgttttt gctggttcac aggttagtct ggaagacttc
     1921 ctagccttgc tccattctgg aatgagtttg tcccaatacc gtaccattcc agcaacagtg
     1981 gacactgttc tggtgcagag ttacgatttg attgcaccat tgactgctga ctttgtctat
     2041 gctattggac taactcagga caatttacca aaaatttctc aaaacaccag tcttctgaca
     2101 gatgaagaaa ggcaaaatct aaaccaagcg accgaagaag gcgttcaatt actgattgcc
     2161 agcagtgaaa atctcaagaa aaatcgctac actatgcttt ccttggtcaa ttctgctcgt
     2221 aagcagttgt tcttgtcggc tccaagcctt tttaacgaaa gtgaaagtaa ggaatctgcc
     2281 tatcttcaag agttgatcca ttttggattt aggcggagag agaagaggat gaatcacaaa
     2341 ggactgtcta aggaggatat ggggtcctat cacagtcttt tgtctagtct ggttgcctat
     2401 caccagcagg gtgagatgag cgatactgag caagatttga cttttgtcaa ggttctgtcg
     2461 cgtgtcatag gtaaaaaact agatctgcaa ggtctggaaa atccagccat cccaatcagt
     2521 ccaagcagca agaccttagc caaggacacc ttgcaagctc tctatcctgc caaacaggag
     2581 ttttacctgt ctacgtcggg tttgacagag ttttatcgca atgaatacag ttatttccta
     2641 cgctacgttt taggcttgca ggaggaatta cgtttgcgtc ctgatgcccg tagtcacggg
     2701 aatttcttgc atcgtatctt tgaacgcgcc ttgcagttgc ctaatgaaga ttcctttgac
     2761 caacgtctag aacaagctat tcaagaaacc agtcaagaac gcgaatttga agctatttat
     2821 caagaaagtt tggaagccca gtttaccaag gaagttttgc ttgatgttgc acggacaact
     2881 ggacatattc tccgccataa tccagtcatc gaaaccatta aagaagaggc aaatttcggt
     2941 gggaaagagc aagcctttat tcaattggac aatggacgca gtgtctttgt acgaggtaag
     3001 gtggaccgga ttgaccgttt gaaagctgat ggagcgatag gagtagtaga ctacaagtcg
     3061 agtctaactc agttccagtt tcctcatttc tttaatggac tcaattccca actgccaacc
     3121 tatctagctg ccctaaaaag agaaggggag cagaactttt tcggtgctat gtacttggaa
     3181 atggctgagc ctgtccaatc tttgatggcc gttaaaagtc tggcaggtgc agtagtagaa
     3241 gccagcaagt caatgaaata ccaaggcctc tttttagaaa aagaaagcag tcatttgggc
     3301 gaattttaca ataaaaacaa ggccaatcag ctgacggatg aggaattcca gctcctactg
     3361 gactacaatg cccatcttta caagaaagcg gctgagaaga ttttagaagg ccagttcgct
     3421 atcaatccct ataccgaaaa tggcagaagc attgctccat gcgtccagca acatcaagcc
     3481 atcacaggct ttgaagccaa ttaccatttg ggtcaagccc gtttcctaga aaagttggac
     3541 ttagctgatg gcaagcgtct ggttggagaa aaactcaagc aagcttggct tgaaaaaata
     3601 agagaggagt tgaatcgatg aagcttattc cctttttaag tgaggaggag attcaaaaac
     3661 tgcaagaagc agaagcaaat tcgagcaagg aacagaagaa aactaccgag caaatcgaag
     3721 ctatctacac ttctggccag aatatcctgg tctcagcatc ggctggttct ggaaagacct
     3781 ttgtcatggc agagcgcatt ctgaaccaat tggcgcgtgg tgtcgaaatt tctcaactct
     3841 ttatctcaac ctttaccgtc aaggctgcaa ctgaacttaa agaacgttta gagaaaaaaa
     3901 tcagcaagaa aatccaagaa acagatgatg tcgacctcaa acaacacttg ggtcgccagt
     3961 tggcagacct acccaacgct gccatcggaa ccatggactc tttcacacaa aaattccttg
     4021 gcaaacatgg ttatctgctt gatattgcac ctaatttccg tattttacaa aaccaaagcg
     4081 agcaacttct tctcgaaaac gaagtctttc atgaggtctt tgaagcgcat taccaaggta
     4141 aacagaaaga gacctttagt catttgctga aaaactttgc tgggcgtggc aaggacgaac
     4201 ggggtctgcg ccagcaggtc tataaaatct atgacttcct ccaatccacc agcaatcctc
     4261 aaaagtggct gagtgactct ttcctcaaag gctttgagaa agctgatttt accagtgaaa
     4321 aagaaaaact gactgagcaa atcaaacaag ccctttggga tttggaaagc tttttccgtt
     4381 accatctgga taacgatgcc aaggagtttg caaaggctgc ctatttagaa aatgttcagt
     4441 taattctgga tgaaattggc tccctaaatc aggagttcga tagtcaggct tatcaggcag
     4501 tacttgcgcg tgttgtcgcc atctctaagg agaaaaacgg tcgtgctctg actaatgcca
     4561 gccgtaaggc tgatttgaag cccctggctg atgcctacaa cgaagagaga aagacccagt
     4621 ttactaaact aggacaatta tcagaccaga tagcgattct cgactatcaa gaacgttatc
     4681 atggagacac ttggaaacta gctaaaacct tccaatcttt catgagcgat tttgtggagg
     4741 cttatcgtca gagaaaacga caggaaaatg cctttgaatt cgctgatatc agccattaca
     4801 ccattgagat tttagaaaat ttcccacaag ttcgtgagtc ttatcaggag cgcttccatg
     4861 aagtcatggt cgatgagtat caggatacca accatattca agaacggatg ctggaattgt
     4921 tgtctaatgg ccacaatcgc tttatggtgg gagatatcaa gcaatccatc tatcgtttcc
     4981 gtcaggcaga cccgcagatt ttcaatgaga aattccaacg ctatgcgcaa aatccccaag
     5041 aaggcaagct cattctcctc aaggaaaatt tccgtagtag ttcagaagtg ctgtcagcaa
     5101 ccaatgatgt ctttgaacgt ctcatggacc aagaggtcgg cgaaatcaac tatgataaca
     5161 agcaccagct tgtttttgcc aataccaaac tgactcccaa tccagacaac aaggcagaat
     5221 ttctcctcta cgacaaggac gatacaggtg aggaagaaga gagtcaaaca gaaacgaaac
     5281 taacaggcga aatgcgctta gttatcaagg agattctgaa acttcatcaa gaaaaaggtg
     5341 ttgcctttaa ggaaattgcc cttctgacct ccagccgcag tcgtaatgac cagattctcc
     5401 tcgccctgtc tgagtacggg attcctgtca aaactgacgg agagcaaaac aattatctcc
     5461 aatccctaga agtgcaagtc atgctagaca ctcttcgtgt cattcacaat cccctgcaag
     5521 actatgcctt ggttgccctt atgaagtctc caatgtttgg ttttgatgag gatgagctag
     5581 cacgtttgtc ccttcagaaa gcagaggata aagtccacga aaatctctat gagaaactgg
     5641 tcaatgcaca aaaaatggca agtagtcaaa aaggcttgat tcacacagct ctagctgaaa
     5701 aactaaagca attcatggat atcctagctt cttggcgctt gtatgccaaa acccactctc
     5761 tctatgactt gatttggaag atttacaacg accgttttta ttatgactat gttggggctt
     5821 tgccgaatgg tcctgctagg caggccaatc tctatgccct agcactgcgt gctgatcaat
     5881 ttgaaaagag caatttcaaa ggtttgtcgc gttttattcg tatgattgac caagtcttag
     5941 aagcccagca cgatttggca agcgtggccg tcgcaccgcc aaaagatgca gtagagctca
     6001 tgaccatcca caagagtaaa gggctggagt ttccttacgt ctttatcctc aatatggatc
     6061 aagatttcaa caagcaagac tctatgtcag aagtcattct cagtcgtcag aatggtcttg
     6121 gtgtcaaata tattgccaag atggagacag gggcagtaga agaccactat cctaaaacca
     6181 tcaaactctc cattcctagt ctgacctata ggcagaacga agaggaatta cagctagcaa
     6241 gctattctga gcagatgcgt ttgctgtatg ttgctatgac gcgggctgag aaaaagctct
     6301 atcttgtcgg caagggttct cgtgaaaagc tggaagtcaa gcaataccca gcagccaaaa
     6361 atgggaaact aaatagcaat actagactgc aagcacggaa tttccaagat tggctttggg
     6421 ctatcagtaa agtgtttact aaggacaagc tcaactttag ttatcgtttt attggcgaag
     6481 atcagttgac cagagaagct atcggagagt tggaaaccaa gagtcctctc caagatagct
     6541 cccaagcaga caatcgtcag tcagatacca tcaaagaagc tctggaaatg ctgaaggagg
     6601 tggaagttta taatactctt caccgcgcag ctattgaact tcctagtgtt caaaccccaa
     6661 gtcaaatcaa gaaattctac gaaccagtta tggatatgga aggtgtcgag attgctggtc
     6721 aaggtcagtc agtaggcaag aaaatcagct tcgatttgcc agatttttca accaaagaaa
     6781 aggtaactgg agctgagatt ggtagtgcta ctcacgaact catgcagaga attgacctca
     6841 gccagcaact aacccttgct agcctaacag aaacactcaa acaagttcaa actagccaag
     6901 ctgtcagaga caagatcaat cttgataaaa ttcttgcttt ctttgacaca gtactcggtc
     6961 aggaaattct tgctaatacc gaccatctct atcgcgagca acctttctcc atgctcaaac
     7021 gagaccaaaa gagtcaggaa gactttgttg tccgtggtat ccttgatggc tatctgcttt
     7081 acgaaaacaa aattgttctg ttcgactaca agacagaccg ctatgatgaa ccaagtcaac
     7141 tcgtagaccg ctatcgtggt cagttagctc tatacgaaga ggctttatca cgagcctatt
     7201 cgattgaaaa tattgaaaaa tacttgattt tactcggtaa agacgaggtt caagttgtaa
     7261 aagtataact tatactcttt gaaaatctct tcaaaccacg tcagcttcgc cttgctgtat
     7321 atatggttac tgacttcgtc agttctatcc acaacctcaa aacagtgttt tgagctgact
     7381 tcgtcagttc tatccacaac ctcaaaacag tgttttgagc tgacttcgtc agttctatct
     7441 acaacctcaa aacagtgttt tgagctgatt gcggctagct ttctagtttg ctctttgatt
     7501 tttattgagt attagaaagg agacctcatg acacttccag ttagaaaatc cctgcacgat
     7561 gcagttttac aggcttcgaa agcggatact tgggaacaag ctaccaagga atggaatgaa
     7621 gtttccttga tttttaacgg tatcggccgt agcaattgtg tctgtggaaa tgccatcaaa
     7681 tacgcttatg aactctttaa cggtgtcaca ggccaacgcc tctttccaat tggtagcgac
     7741 tgtgttcgtc attttcatcg attgtccctt gaccagcaat tggaagagga agaaaaactg
     7801 ctcagaaagg ttgaaaacct aaccagaaaa gctcagaaaa aggaaaaaat caaggtcaat
     7861 aaaagtgact ttgacgagcg acttctaaaa tggctctggg aaaaaggtgt tttcaaaccc
     7921 aatcgtggca atcaatttgc gcctgagaga gactaccagc ttttcctaga agtctttcag
     7981 ggaggaagtt ggaccaaggc ggaaccaaag aaaaaggctc ggatggaaga agtccttgaa
     8041 aagtgtatca aacccttttt acttgggaaa tcggatgacc aactctacct tgtcaagcta
     8101 ggcaaggaga aaattgacta cgaacaagaa ctccgtatcc aggcagagaa agaacgcaag
     8161 aagagggata aaatcgccaa gcaatatgca gacaatctgg ttctagccat gggacccgca
     8221 gagcgtgctt atcaagatta ctttggcttt acagagaccc tgactcaaga agaacgcaag
     8281 tgggaaaaaa ttctttttgg taaaaataga gcagaacggg ctatcaaggc caaacaatac
     8341 caaaaagagc tggaaaaaga ccaacgaatt gccagtcagg atccaattga aagaaaacag
     8401 aagcagactt ggctcctcaa ttcctatttt cgtgagcttc ctgaagaaaa agctcgattt
     8461 tctaggctct tattagaata tcgaaaaagt ggtgaagtac ccttttcaac tgaatacctg
     8521 tccgatcatc tcatcgactt tttctataaa atgaaagcct ttgaatttga aatcgcacca
     8581 gaacaagtcc gagattttct aaaaaaaagc ctgcaggaag accatcgctc atcagcacaa
     8641 ggaagctgga tagaaggaat tctcctcaac tgcctcaaac catttttaga acgattagtg
     8701 atatgagtaa cacctaccgt gtaaatttca tggtaggtgt tattgtaccc ccaaataata
     8761 taaaacaaag tatgaaagcg catctcaatt ttaagacttt tttgtaaatt aagagtataa
     8821 ttaaggtata ctgccttttc tataga
//
LOCUS       DAVKJD010000072         8732 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKJD010000072 DAVKJD010000000
VERSION     DAVKJD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.072"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..524
                     /locus_tag="VW157_001666"
     CDS             <1..524
                     /locus_tag="VW157_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEU9552935.1"
                     /translation="IELIKEDLIINESVLNVDKKGYELICLQHPVSYDLRRIISVIKI
                     STDIERIGDRIVEILKNLQIIQNNEILKKIISEIKILHEVIGLHMNRAISCYREEQSG
                     CLDMVVIQKQNEIEELSTNIEKKIMNYIFEDDGNVSEVIGALDIIHHLDKIAHTTQSI
                     YKWIMYRKYGNIN"
     gene            537..2012
                     /gene="galT"
                     /locus_tag="VW157_001667"
     CDS             537..2012
                     /gene="galT"
                     /locus_tag="VW157_001667"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9552936.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGTDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFRFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEENAIADYHLEWADQLKEKYPRINKEEVN
                     NVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            2025..3035
                     /gene="galE"
                     /locus_tag="VW157_001668"
     CDS             2025..3035
                     /gene="galE"
                     /locus_tag="VW157_001668"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9552937.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSILVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            3219..3878
                     /locus_tag="VW157_001669"
     CDS             3219..3878
                     /locus_tag="VW157_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9552938.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRVTEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVRFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFIKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3903..4970
                     /locus_tag="VW157_001670"
     CDS             3903..4970
                     /locus_tag="VW157_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9552939.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIPGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSISGLQGGTGRAILASILVRYLDDKGELGVSEKGWEVAKEYLKNAYTL
                     QKGESSIVKMLDKEDPIQYGMMWGSGALVGQKEQNVVFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKNFGSIPANKDALKDLPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            5073..6083
                     /locus_tag="VW157_001671"
     CDS             5073..6083
                     /locus_tag="VW157_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552940.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            6095..7786
                     /locus_tag="VW157_001672"
     CDS             6095..7786
                     /locus_tag="VW157_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9552941.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7789..8499
                     /locus_tag="VW157_001673"
     CDS             7789..8499
                     /locus_tag="VW157_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9552942.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2977 a         1186 c         1703 g         2866 t
ORIGIN      
        1 gaatagaatt gattaaagaa gatttaatca taaatgagtc agttctaaat gttgataaaa
       61 aaggatatga gttaatttgt ttacaacatc ccgtttcata tgacttaaga agaattatta
      121 gtgtaataaa aatatcaact gacattgaac gtattgggga tagaattgtt gaaattttaa
      181 aaaatctaca gattattcaa aataatgaaa ttttgaagaa aattatttca gaaataaaaa
      241 tacttcatga agttatcgga ctgcatatga atagggctat atcttgttat agagaggaac
      301 aatctggatg tttagatatg gttgttatac aaaaacagaa tgagatagaa gagcttagta
      361 caaatattga aaagaaaata atgaattaca tatttgaaga tgatgggaat gtttctgaag
      421 taattggtgc tttagatatt attcatcacc ttgataaaat tgctcatact acgcaatcaa
      481 tttataaatg gataatgtat agaaaatatg ggaacataaa ttagaaagaa aaatatatga
      541 aaacaattat tgataatttt gttgatagag taattgaata cggaatgtat aatgaaattg
      601 acaaaatata tgtgaaaaat agagtactag cgttaatagg tgaggaggga actgatcgca
      661 tttccgatga aaatgacctg aaacaaataa aagattactt agtggaaatc gccttaaaaa
      721 acggtaaaat taaagattta atcgaggaaa aggaatgttt aggagcagag ttaatgaatt
      781 tcatagttcc tttacctagt cgattaaatg atattttttg gagttcatat gatatatcac
      841 ctcaagaagc agttgaagag ttttataagt taagtaaaga tagtgattat attaaaactt
      901 ctgctattgc taagaatatt gagtttcgag catcaactaa atacggagag ctagagatta
      961 cgataaattt gtcaaaaccc gaaaaagatc caaaaacaat tgctgctgaa aaattagtaa
     1021 aagccactaa ttatcccaaa tgtttactat gtatggaaaa tgaaggatat caggggcgaa
     1081 taaattatcc agcacgttcg aatcatagaa ttattcgatt gaaacttggt gacgaagtat
     1141 ggggattcca atattctccc tacttatact ttaatgagca tgcgatattt ttaaatagtc
     1201 aacatgtacc aatggctatt acctctaaaa catttgaaca attattggag attgttgata
     1261 ttttgccagg atattttgct ggttcaaatt ctgatcttcc tatctcagga ggctccattc
     1321 ttagtcataa ccactaccag ggagggaaac atattttccc aatggaaaaa gcaaaatttg
     1381 agagtgaatt tcgttttaaa gactttgagg atgttaatgc tggtattgta aaatggccaa
     1441 tgtcagtaat taggttacaa agtgaaaata aaaatcgctt gttagattta gctactaaaa
     1501 ttctaaataa gtggagagaa tattcagatt tagaagtaga catcattgct atgactgaag
     1561 atgtcccaca ccatacggtg actcctattg ctcgtaaagt ggacgggaga tatgaattag
     1621 atattgttct aagagataat catactactg aacagtaccc agatggtgtt ttccatcctc
     1681 atcaagatgt acaacatatt aaaaaggaaa atatcgggtt aatcgaagtg atgggacttg
     1741 cgatattacc acctcgtttg aaaccagagt tagaagaagt gggaaaatat cttttaggag
     1801 aggaaaacgc aattgcggat tatcatttag aatgggccga ccaattgaaa gaaaaatatc
     1861 ctcgcattaa taaagaagag gttaataacg tagttcaaca cgaagcagga caagtgtttg
     1921 cgagagttct ggaagatgca ggagtttata agaatacacc gtctgggcat gaagcgttta
     1981 tgagatttgt taagtcggtt ggaattaatt aggaggaaac ccaaatggca atattggtaa
     2041 caggcggagc tggttatatt ggtagccata ccgtagtaga attactaaat ttaggaaagg
     2101 aagtcatcat tgtcgacaac ctttcgaact ctagcatctt ggtgctagac cgtattgaag
     2161 caattacagg aatacgtccc gtgttttacg aattagatgt ttgtgataaa caagcattga
     2221 gaaaggtatt tgaacaagaa tcgattgatg ctgcaattca ttttgcaggt tataaagctg
     2281 tcggagaatc cgtgcaaaag cctgtgatgt actacaaaaa taatattatg agtacattgg
     2341 cacttgttga agtgatgtca gaatttaatg ttaaaaagat tgtcttttct tcaagtgcga
     2401 ctgtatatgg aattaacaat cagtcacctc taattgagac gatgcaaaca agtgcgacaa
     2461 atccttatgg gtatacgaaa gtgatgcttg agcaaatttt aaaagatgtt catgtggcag
     2521 attcagaatg gagtattgcg ttgcttcgtt atttcaatcc aattggtgct catgagtctg
     2581 gtttaattgg ggaagatccc tcaggaattc ctaacaactt gatgccttat attgcacaag
     2641 tagcggtagg taagctgtca gagctaagtg tgttcggaaa tgattatgat acgcttgatg
     2701 gtactggtgt gcgtgattat atccatgtag tagatttagc aataggacat ataaaagctt
     2761 tagaaaaagt atctgaaaaa acagatgttt atatctataa cctaggctcg ggagaaggca
     2821 ctagtgtatt acaacttgta aatacatttg aaagtgttaa taagatccct attccttata
     2881 aaatagttcc aagacgttca ggagacgttg caacttgtta tgcaaatgca gacaaagcat
     2941 ataaggaatt aaattggagg acaacaaaat cgattgaaga catgtgtaga gatacatgga
     3001 attggcaatc aaaaaatccc aatggctata attgatgata ctgtttatat gagtgctgta
     3061 ttttggaaaa attacttaac cttttaatga aattcatatt taatgaaaga taaatttttt
     3121 taaaaaaatt tttgaaagcg cttgacaaag gaaacggttt cattgtataa tgtaaacatg
     3181 atgacaccga tgtcaaatat aaaaaagaag gtctttatat gaaaaactta acaaaaatta
     3241 agttcaaaga gaatggggaa tttaatcatt tccctgggaa tacagttgta gcaaatcttt
     3301 atactaaaca agatttgatg gaagttgttg atattattca atcacgttat agagaattac
     3361 catttatcga taagtttact ttaactccaa ggaattctat tcacatgact gtaattgaat
     3421 tgttgtgcca tgaaaatcgt gaaacggagt tttggagcag taatcttcct ctagatacac
     3481 ctttacagga aatacacgat tactttgcta aacaacttga aatttttcca ttgttggatg
     3541 aagaaattca tatgcgtgta actgaaatgg gaaaacaaaa catactagtt gaacctgcag
     3601 atgaagcttc tgcaaagaga ttagaagaaa ttcgtactta tgtctcagaa aaagcaggtg
     3661 ttcgtttccc taatcatgat agatatcaat tccatatttc aattgggtat cttcggattc
     3721 ctctaaccga agaggaggaa gaagagttta ttaaagtcag agcagaatta actgaaattt
     3781 tattagagaa gattccaact attactgtaa accgtattga ctatactgta tttgaagata
     3841 tgagacaatt tgttccatat cacgaaaaat ttaaataaaa taataaaaaa ggagaactta
     3901 tcatgaaaaa aatgaaagtt tggtctactg tacttgcaac gggagttgct cttactacac
     3961 ttgctgcttg ctctggaggt tcaaattcta cgactgcttc ttcatctgaa gaaaaagctg
     4021 ataaaagtca agaactagta atctattcga actcagtctc aaatggtcgt ggtgattggt
     4081 taactgctaa agcaaaagaa gctggtttta atatcaaaat ggttgatatc cctggcgctc
     4141 aattagcaga ccgtgttatt gctgagaaga ataatgcagt tgcagatatg gtatttggaa
     4201 ttggtgctgt tgattcaaat aaaattagag atcaaaaatt actagtacag tacaagccta
     4261 aatggttaga taaaattgat caatctttat cagataaaga taattattat aatcctgtga
     4321 ttgttcaacc attagtttta attggggcgc ctgatgtaaa agaaatgcct aaagattgga
     4381 ctgaattagg tagtaagtat aaaggtaaat attcaatttc tggtcttcaa ggaggtacag
     4441 gacgggcaat tctagcaagt atcttagttc gataccttga tgataaaggt gaattaggtg
     4501 tttccgaaaa aggttgggaa gtagcaaaag aatatttgaa aaatgcatac actcttcaaa
     4561 agggagaaag ttcaattgtt aagatgttag acaaagaaga tccaatacaa tatggaatga
     4621 tgtggggttc tggtgcatta gttggacaaa aagaacaaaa tgttgttttc aaagttatga
     4681 ctcctgagat tggtgtacca tttgtaactg aacaaactat ggttttaagc actagtaaaa
     4741 aacaagcgtt agctaaagaa tttattgatt ggtttggtca atcagaaatt caagtagaat
     4801 atagtaagaa ctttggatct attcctgcaa ataaagatgc cctcaaagat ctaccagaag
     4861 atactaagaa atttgttgat caagtgaaac cacaaaatat tgactgggaa gctgttggaa
     4921 agcatttgga tgaatgggta gaaaaagctg aattagaata cgtacaataa gaaataagag
     4981 ttaattttta tgtccaagtc tctctattat ttctaaattt atggaagaaa taaagagagg
     5041 cttttggttt agtaatagag ggagttagtg agatgattaa atttgataat attcaaatta
     5101 aatatggtga ttttgttgca attgataatc tgaatttaga tatacatgaa ggggaatttt
     5161 ttacatttct tgggccttca ggatgtggta aatcaactac tttgagagca ttggtaggtt
     5221 ttctagatcc atcatcagga agtattgaag ttaatggaac agatgtcact catttggaac
     5281 ctgaaaagcg tggaattggt attgtatttc aatcttatgc gctatttcca actatgactg
     5341 tttttgataa tattgcattt ggtttaaaag ttaagaaggt agctccagat gttattaaag
     5401 ctaaagtatc agcagtggca gcaaaaatta agatctctga tcaacagtta cagcgtaatg
     5461 tatcagaatt atctgggggt caacaacaaa gggtagcatt ggctcgtgct ctggttcttg
     5521 aacctaaaat tctttgtcta gatgaaccat tgtcaaacct tgacgcaaaa ttacgtgtag
     5581 atttgagaaa agagttgaaa agacttcaaa aagagttagg tattactact ttatatgtta
     5641 ctcatgatca agaggaagcc ttgactttat ctgatagaat tgcagtcttt aacaatggat
     5701 acatcgaaca ggtcggtaca ccagtagaga tttatcataa ttctcaaact gaatttgtat
     5761 gtgattttat tggagatatt aatgttttga ccgatgaaac agtccacgaa gtattattga
     5821 aaaatacaag cgttttctta gaggataaaa aaggatacat tcgattagag aaagttcgat
     5881 tcaatcgtga aactgaacaa gattttattc taaaagggac aattattgat gttgagtttt
     5941 ctggagttac aattcactat acaataaaag tttctgaaag tcagattctt aatgtaacaa
     6001 gtattgatag tcaggctgct attagatctg tcggagaaag tgtggaatta tttatcacac
     6061 catcagacgt tctgcaattt taaggaggtg cagtatgcgt cataaattaa atttaaaaga
     6121 ttggcttatt cgtttagggt taatctggtt cttagtaaca tttattattt atccaaactt
     6181 tgatctagta gtgaatgtat ttgtaaaagg aggagaattt tcccttgatg ctgtacatcg
     6241 tgttctaaaa tctcagaggg cacttcagag tattatgaac agttttaagt tagcattttc
     6301 actcattatt acagttaatg tcgtaggtat tctttgtgtt ctatttacag agtactttga
     6361 tattaaaggt gctaaaattt taaaattagg ttatatgacc tctttaattt atggaggagt
     6421 ggttttagcg actggatata aatttgtcta tggtccttat ggattgatta caaaattttt
     6481 acaaaatgtt atcccttctt tagaccctaa ctggtttatt gggtatggtg cagtcttatt
     6541 cattatgaca ttttcaggaa ctgctaatca tacattgttt ttaacaaata caattcgaag
     6601 cgttgactat cacactattg aggctgctcg aaatatggga gcaaaaccat ttactgtttt
     6661 ccgaaaagta gtgttaccaa ccttaattcc aactctattt gcacttacta ttatggtttt
     6721 tcttagtggt ttatctgcag tagcagcacc catgattgtt ggtggtaaag aatttcaaac
     6781 tataaatcca atgattatta catttgcagg gatggggaat tctcgtgatt tagctgcctt
     6841 acttgcaatt attttaggta ttgcaactac aattttgctt actatcatga ataagataga
     6901 aaaaggtgga aattatattt ctatctctaa gactaaagcg cctcttaaaa aacaaaaaat
     6961 tgcgtctaag ccttggaata tcattgctca cattgtagca tatggattgt tcacagtttt
     7021 catgcttcca ctaattttta tagtattata ctcatttaca gatccagttg caattcaaac
     7081 aggtaactta acattatcaa actttacttt agaaaattat cgcttattct ttagtaatag
     7141 tgcggcattc tctccattct tggtcagctt tatttattct attattgctg cgacaacagc
     7201 aacaattctc gcagttgtat ttgctcgtgt tgtcagaaaa cataaatctc gttttgattt
     7261 cttgtttgaa tatggtgctc tacttccttg gttactacca agtacacttt tagcagtaag
     7321 tttattattt acttttaatc agccacaatt tcttgtcttg aatcagattt tggtaggtag
     7381 tttggtaatt ctacttattg catatatagt tgtaaaaatc ccattttctt atagaatggt
     7441 acgtgctatt ttatttagtg ttgatgatga gatggaagat gcagcaagaa gtatgggtgc
     7501 ttcacctttt tatactatga tgaaggttat cattccattt attttaccgg ttgttctctc
     7561 tgttattgct ttaaacttta actctttatt aactgacttc gacttatctg tattccttta
     7621 ccatccccta gctcaaccat taggtattac gattcgatct gcaggtgatg aaacagcaac
     7681 atctaatgca caagctctgg tatttgttta tacaattgtt ctgatgatta tttctggaac
     7741 ggtattatac ttcacacaaa gaccggggcg taaagtaagg aaataatcat gacagccact
     7801 agtcttgggt tatcaaatat tgaaatagtt gtcaggattg ttttatcagt agtcattggt
     7861 agtataattg gtttagagag agggagcaaa tcccagcctg caggcatccg aacttatagt
     7921 attgtttgtc tagctgcatg tttgattatg atgacgaatg aatacgtatc ttataaattt
     7981 gggacaggag atcctacacg attaggagct caagttatat caggtgtggg ttttctaggc
     8041 gctggaacga ttcttattac agataaaaag aaaattacag gtctgacaac tgcagcaggc
     8101 atttgggctt cggcaggaat tggattagct attggagtag gtttttatga gggagctctt
     8161 ttagtagcta tttctgtttg gggtgtgata tccatgttcc aaccactaaa aaaatatctg
     8221 caaaatcgtt ctaaaatgat tgaattgtat atagtggtta aatctacaga agcctataat
     8281 cgtgtattgg tttattgtgc agaaaatggt atcagaatga ccgattcaag aacagcgttt
     8341 ggagatgtta attctgacag aattgaatac tttgatgttc cggacaaaaa aatagcgtca
     8401 tttattactc tggaattatc aggtagattt gaacatcttc gactgatgga agaaattgct
     8461 aatattgtag gtgttattta tgtcgaagaa attagctgaa atttagtaaa caaagggaca
     8521 gatgtttttg tccttttgct ttttagatgt tttaatcatc taatttttga gtagattttt
     8581 gctcagaggt ttttatgaaa gattataaaa tcaattttga tttaggtaaa atagaatatt
     8641 ttgataataa ttgtttgata caagtataca aatttatttc tttttatgat atttgtgaaa
     8701 tggtgtttgc ctttcattta cctcctgatg ag
//
LOCUS       DAVKJD010000073         8619 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKJD010000073 DAVKJD010000000
VERSION     DAVKJD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.073"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            249..1172
                     /locus_tag="VW157_001674"
     CDS             249..1172
                     /locus_tag="VW157_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9552943.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPDKKQASTNFKPAGQSIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRK
                     SRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLSNVFSEEHIYYAHP
                     YASWERGTNENHNRFIRRWLPKGTKKMTPKEVAFIEKWINNYPKKCLNYKSPREDFLM
                     ANLNLKFSVRNKSRNRFKFCRSFKYPACGCS"
     gene            1150..1794
                     /locus_tag="VW157_001675"
     CDS             1150..1794
                     /locus_tag="VW157_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9552944.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATSNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAVVSREKLLLDQDRELAKKSLDAAYIQNEEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(1836..3824)
                     /locus_tag="VW157_001676"
     CDS             complement(1836..3824)
                     /locus_tag="VW157_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9552945.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMNVVIAVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKRGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSDIYNQFYGGMNVSISEEEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSVLFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3826..4584)
                     /locus_tag="VW157_001677"
     CDS             complement(3826..4584)
                     /locus_tag="VW157_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9552946.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVMTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            complement(5555..5827)
                     /locus_tag="VW157_001678"
     CDS             complement(5555..5827)
                     /locus_tag="VW157_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9552947.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5886..6335)
                     /locus_tag="VW157_001679"
     CDS             complement(5886..6335)
                     /locus_tag="VW157_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEU9552948.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            complement(6322..8451)
                     /locus_tag="VW157_001680"
     CDS             complement(6322..8451)
                     /locus_tag="VW157_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9552949.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
BASE COUNT         2622 a         1809 c         1667 g         2521 t
ORIGIN      
        1 attgaaacta gaatagtaca cctctgcttc taaaacattg ttagaaatag attttactgt
       61 cctgatcgat ttgttctgtt cttgtttcat tttactatat gttcgtaaat gaaagttcta
      121 taatgaagtt agccacccag ccctcctaaa aattagatgg ttcaactagc cgatagctct
      181 tgtaggcata agaaaacacc tctgtgttat acttgttgtt caaccacaaa cacaagaaag
      241 gcacagagat gcaagacaat tatactacaa aagccaaaca tttgacaatc gatagccgtc
      301 gcttaatcga aagatggaaa aaagaaggga aatcaaatag agaaattgcc tctctacttg
      361 gaaaagctcc tcaaactatc cacactgaaa tcaagcgtgg gacagtccga caatgtcttg
      421 gaaaagggcg cttcaaagag gtttattctg ccgactatgc tcaacagtct tatgaaaaca
      481 atcgcaagcg ctcggtcaag aaatcaagct tgaccaagga actaaaggaa aagattctcc
      541 actatcataa ccaaaaattt tcgcctgata agaaacaggc tagcaccaac tttaaacctg
      601 ctggtcaatc catcgaacag cggtctgaag ctatcaatct tcgcttggag aatgggcatt
      661 atgagattga tacggttcta cttacgagag cgaaaaacta ctgcttgctt gtcttgacgg
      721 atcgaaagag tagacatcag atcatccgat tgattccaaa taaaagtgct gaggtggtca
      781 atcaggctct aaaactcatc ttaaaacaac acaagattct ttccatcacg gcagataatg
      841 gaacggaatt caatcgcttg tctaatgtat tttctgagga gcacatctat tatgcgcacc
      901 cctatgcctc ttgggaaagg ggaactaatg agaatcacaa caggttcatt cgtagatggt
      961 tacctaaggg aaccaagaaa atgactccca aagaagtcgc attcatcgaa aagtggatta
     1021 acaactatcc taaaaaatgc ttgaactaca agtcacccag agaagacttc ttgatggcta
     1081 acttgaactt gaaatttagc gtcagaaaca aatcacgaaa tcgattcaaa ttttgcaggt
     1141 cgtttaaata tcctgcgtgc gggtgttctt gatgctaacg atggaattat ttccattgct
     1201 ggtgtggtta tcggagttgc cagtgccacg agcaatatct ggattatctt tttatcaggt
     1261 tttgcggcta tcttagctgg tgccttttca atggctggtg gagaatatgt atccgtttca
     1321 actccaaaag ataccgagga agctgtcgtt tcgcgagaaa aactcttgct agaccaagat
     1381 agggaactag ccaaaaaatc cctcgatgct gcttatatcc aaaatgaaga attcaaaact
     1441 tctgcccaac tcttgactaa taagatcttt cttaaaaatc cactcaaggc tctggtagag
     1501 gaaaaatatg ggattgagta tgaagaattt accaatcctt ggcacgctgc catttctagc
     1561 ttcgttgcct ttttccttag aagtttgcct ccaatgctgt cagtgaccat ttttccaagt
     1621 gattaccgca tccctgctac cgtccttatt gtcggtgtgg cccttcttct cactggttac
     1681 acaagtgcta gacttggaaa agccccaacc aaaacagcta tgattcggaa ccttgctatt
     1741 ggtctcttga ccatgggagt taccttcctg ctcgaacaac ttttcagcat ttagaataca
     1801 agaaatacct cgattttgaa gtcgaggtat cttttttaca tttgcacaat cttgcgataa
     1861 cttcttgaag taatcatgaa aatcagcaca taggcgatga ggaagatagc gcagatagac
     1921 aaggtcacaa tcaacatcat agtcgtatcc agtacaccaa tcacttttaa aatcaggcta
     1981 agcatatggt aggcaaaggc gagatgtatg aaggcaaaga gcaaaggaag gaagaaaaca
     2041 gttaaaacct gtttgttgat ggtttgcttg atttgctttt gatctaaacc gactttctgc
     2101 aagataataa agcgttcacg gtcttcatag ccttcagaaa tttgtttgta gtagatgacc
     2161 agaacggttc cgaccataaa gataatggat aggaaaatac caataaagaa gacaccgcca
     2221 aagaggacac tcatttgagc actagcatct gctagagtgc taccatacac atagttacct
     2281 tcagtgttta attgagcatt aaacttttgt aagtattttt catattcttc agcgaccttg
     2341 agttgctctt cttcactgat acttacattc ataccaccgt aaaactgatt atagatatcc
     2401 gaatctggga attgatccaa aaaggcttgt aaatcaggaa caacaaggta attgtaatca
     2461 gtagtcaaga tattaaactt atttggaaca tggttcacaa tgaaatcttt attaaattct
     2521 tctttgacag aaaattgatg atcatttaga gttagagctt tctgtccttt cagtccgtca
     2581 tttttggcaa agagaccgac ctcatttcct gatagagaca gtttttgacc agtcatattt
     2641 tcataatctt tttggtcaaa taccatgaaa actgttttgg gttggacacg gttttgtcct
     2701 ttttcaaaaa tagttaactt ggttccttct tgatttgcaa taccaaagtt actgtaacga
     2761 agtacttctt tctctttgac actataacct ttgtcacttg caaactggct caagagtttg
     2821 tccaaatctt ctttttcaac attttgccct gaaaccccaa aatcatgagg atttagaact
     2881 tttttaaagc tttctgcgga attgaaaatg cttgtcgctg ctgacatggt taccaaaacc
     2941 attgttgaca aaatagcgat ggttgctagt ccaaccgcat ttttcttcat acggaaaatc
     3001 aagttggaaa cagagatgag gttattaggt tggtaatagt atttcttgtt tttctttaag
     3061 atttgtagga agactgtaat ccctgcatta aacaatagat aagtaccaaa gataaccagc
     3121 aaaacagcta ggaagaaagt tgttagggct gtaagaggat cggttaccgt aagggcaaga
     3181 taatagccaa tccctaaact tatggaacca agaatcgttt ggagaggtag gaaacgacct
     3241 cttttctctc cgcttgcttt ctcacgcgag agctggaggg cattcatacg ggcgattcga
     3301 agggcattca ggaacatgag gcctaggaaa atcaatccaa agacaagaag tactgcaatg
     3361 acaacattca tttggaaggt agcaaccagc tcaaccttca atttcattag tttgagcagg
     3421 aaagcgaaaa ttaacttgtc aaacaaggct ccaataccga tacccgctcc aacagttaga
     3481 atcccaaata ccactaactc cttaaaggtc atactgataa gatgacgctt ctccaagccc
     3541 aacatgccat aaattcctag ttccttggaa cggttcttca tgacaaaact attggcatag
     3601 agaacgataa tggctgacgc aagggtgacg acaaacatac caaatccaag tgtagcctga
     3661 atggttgttc ctccacggat ttcagcaatc ttgggattga aggttaggga gtaaaagaga
     3721 taggtgacag tgactgccaa gagaacagcc agcgcaaaag gatagtagag tttgcggttt
     3781 ttaatcaagt tcgataccgc taacttattg gttaatcgaa acatactaat tcacctcgct
     3841 tgccatgaca gtcaaggtat cagagatttc ttggaacatc tgacgttctg tcttctctcc
     3901 acggtagatt tgattgtaaa gaatgccgtc tttgataaag agaacacgct tggccctgct
     3961 agcagctgct gttgagtggg ttaccatgag gatggtttgc ccacgctcat tgatttcatc
     4021 aaagacatca agtaaggctg cagatgactt ggaatcaagg gctcctgttg gctcgtccgc
     4081 aaggagaatt tcaggttctg tgatgatggc gcgggctact gctacacgct gtttctgacc
     4141 accagaaatc tcgtaagggt acttctcttg caattggttg atacccagat tttcagctgt
     4201 catcaccaat ttcttcatca tctccgttat aggtcttctt gacaagacaa gcggaagcaa
     4261 gatattgtcc ttaacagaca gagtatctag caagttaaag tcttggaaga caaatcctag
     4321 cttttcacgc cggaaactag aagcctgtga atttttaata gttgctgtat cagttccgtt
     4381 taggtaaacc tgcccacgac ttggtttatc cagcatagct aggatattga gcagagtgga
     4441 tttaccagaa ccagactcac ccatgatagc aacgtagtca cccttttcta cggtgaagtg
     4501 aatatccttg agggcctcta cttggttgcc ctgaaaacga gttttataaa ttttttgaac
     4561 gtgttttaca tctaaaagtg tcatgagaat ctcctttctt aatatcccat caaatgaaat
     4621 gtagcttgca tcatagtgca tcccagctga atgcgctcaa tttctttgtg acttggtttt
     4681 ctagttttct tatgtaagat ttgtttcatg atgttactcc tttgttcttt atgagtctag
     4741 tttacatcaa aaaaagaccg cttgccataa cctaacctta caaaagagac tttaatctta
     4801 catttttgta agattggggg aaatataagc aaaattactt agaaaatgat ttgatttcct
     4861 atttttaaag ttgtaaaata taagtatgaa aaggcttact ttaaaaagga ggaagcggga
     4921 actaacattt tagtaaggtt acctagataa catgccattc aatttaacac ttgcaagtcg
     4981 ttcaacctat aaggaaagaa ggtgcgcttt aatgaaacat aaagaacata tactcatcgg
     5041 atttctctat ctgctctctc cattcatcgg tcagctctca gttgaacaca cactttatcg
     5101 gtacagaatt taccggcact gcttatgtta tttgctggtt atcagttgtt atcagcatcc
     5161 accatttttc caaaaatgta ttgtctcagc agcaaaaata gattaaaaaa ctgcgtttaa
     5221 attactgctt ttatagtaaa atgaaataag aacaggacaa attgatcagg acaatcaaat
     5281 cgatttctaa caatttttta gaagtagagg tgtactattc tagttccaat ccactatagt
     5341 ttgtggatga agctggcgaa atattattaa tactattcaa aaatcaaagt gcaaactata
     5401 tcaacttcac attgccgact aaattacagc ctgcggctgg cttcctagtt tgcactttga
     5461 tttttattga gtataaaagt aaaaaagctt taaatcaagc agtttagact attgatttaa
     5521 agctttttgc gtattccaaa tcttttgtaa aacctcacca aaaccagcct tcattatcgt
     5581 caatggcttg agcttctttg cgtttacgtg ggcctgttcc gtacatttct gcttcgattt
     5641 cttccttggt tggcatgata gaagctagga tgatatagat aatcacgcca aggccaaagt
     5701 ttgcgactgt aaaaatggca aacagaaaac gtacaagggt aacatcaaag ttccacttgt
     5761 ctgacagacc tgctaaaact cctgaaatca tgcgatttcg tctcatttta taaaacttac
     5821 tgttcatgat atttcctctt tctgctaggt tagacatagt atatcacgga taggctcggc
     5881 tttcatcagt cctcaggctg atttactaat agcaactttc ctcgacaaag tccacagcga
     5941 tagcgtttgg tatcaatcct acgcttacgc tgataagttt gctggcagga ttggcaacga
     6001 tagagcttga ttggcttgga gttactattg ggcaaggatg gtacaaaccg taatccatcc
     6061 actgctttca acagttcctt aaaatcccga tccttgtgtt gatagccttt cccttgaaaa
     6121 tagaggtgat aatgacagag ttcatgtcgg acaattttcc taaaaacgtc caaccccagt
     6181 tcctgataaa ccttgggatt aaaatccaaa tgcccatctt tggggaaaaa tcgcccacct
     6241 gtcgaacgta gacgcctatt ccactggaca tgatggataa aaggtctgcc gaagtcttct
     6301 agtgaaacct gcttgacgta atcagtcagt ttcatttgga gctaggagag acagattaac
     6361 tttttcacgt tcagtatcaa ttttcttaac ccaaacgctc accaaatctc caactgccac
     6421 cacttgacta gggtgtttga taaacttgcg actcatatgg gaaatatgga tgagaccgtc
     6481 ctcatgaatt ccgatatcaa caaaagcacc gaaatcaaca acgttacgca ccactccttc
     6541 tagcttttgt ccaaccacta agtccttgat atctaggaca tcttgacgga gaactggagc
     6601 atcaaaggaa tcacggaaat ctcgacctgg tttgagaaga tctgcaatga tatctttaag
     6661 agtttctgga ccaagatcta actcttgcgc catttccttg actgaaagcg acttgagttt
     6721 gctttgggcc tcttcattca gatccttaat gtccaagcat ttgaagagtt ccttaacggc
     6781 agcgtaattt tctgggtgaa ctcctgtatt atcaaggata ttgctacttt cagggatacg
     6841 gaggaaacca gcagcctgtt caaaagcctt ggcccccaaa cgaggaactt tcttgatttg
     6901 ggagcgtgaa gtgatttttc cctcttcctc acggtatttg acgatatttt cagaaatggt
     6961 tttattgagt ccagctacgt gtgaaagaag agctgggcta gctgtattga cattgacacc
     7021 aacttggtta accactgtat cgacaacaaa gtccagactc tcagatagtt tcttctgact
     7081 gacatcgtgt tggtattgac cgacaccaat tgacttggga tcgattttga ccaattccgc
     7141 aagaggatct tgcaaacgac gggcgataga aatggcagag cgtttttcaa cggtcaagtc
     7201 tggaaactcc tgacgagcaa gttcgctggc agaatagaca gaagcaccac tttcattaac
     7261 gataacatag ctgacttcag ggaaatcttt cagaacttcc gctacaaaag cttcactttc
     7321 acgactggcc gttccatttc caatggcaat aatctctaca ccgtattgac caattaaatc
     7381 tgctaaatct ttcttggctt cttcgatttg acgagctgat gctggtttaa caggataaat
     7441 aacctgagtt gtcagcattt ttcctgttgc atccacgaca gctagcttgg cacctgtacg
     7501 aaaggctggg tcaaacccaa gaaccacgcg ccctttcagt ggagcaacca agaggagatt
     7561 gcgcagattg tcagaaaaaa gttggatagc tccctcttca gctttctcag ttaattctgt
     7621 ccgaatacga cgctcaatag caggcaagac ctttttctta acggattgct gaacaacttc
     7681 atcaatataa gcatttttca ccttgaaacg agtagcaaag aaggcaagaa tacggtccgt
     7741 cgcatgttca aaaccgatct tcaagacacc aagtttctcc ccacgattga gagccaaggt
     7801 acgatagcct tgcatagttc caactgtctc tgaaaaatca taataaatct gaaaaacctg
     7861 cttttcatca agactttcat ccttggcttg agaagtgagt ttagagtgtc tcagcacttc
     7921 ctgataagtc atagaacgca aggtcacatc ttccgataag gcttcgacca aaatatcaac
     7981 tgcaccggtc aaggcttcct tgccagtcgc aaatccttca cagacgaact tttcagcttc
     8041 tttctctaag tcaactatat tctgcaaaat caagcgagca agaggaaaga gtccagcttc
     8101 acgggcaatg gttgccttgg tacgacgctt ttccttataa ggaagataga gttcttcaac
     8161 gtctgctaat ttttcggcaa ctaagatagc ttcttccaat tccttggtca acttaccttg
     8221 ttcttgaatc ttagctaaga cagcttcctt acggtcattg agatttgtca gacttttatc
     8281 caaatcaata atagccttaa tcgccacctc atccagacta ccagtcatgt ctttgcgata
     8341 acgcgcgata aagggaatag tcgccccttc agctgtcaaa cttaaaacgg tatcaatttg
     8401 ctttaacgtc actcccaaat cctgagagat tttttcatat tttttatcca taaatctatt
     8461 ataccacaag ctggaaggct ttctccaatc cttttcatac tcaataaaaa tcaaaaagca
     8521 aactaggaag ctagccgtag gctactcaaa atactgtttt gaggttgtgg ataagactga
     8581 cgaagtcagc tcaaaacact gttttgaggt tgtggataa
//
LOCUS       DAVKJD010000074         8448 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKJD010000074 DAVKJD010000000
VERSION     DAVKJD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.074"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..146
                     /locus_tag="VW157_001681"
     CDS             <1..146
                     /locus_tag="VW157_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9552950.1"
                     /translation="SIIRQALKNTEYKDQISSTVLPDIKIKLPIDSRGTPDWNYMERY
                     RDR"
     gene            112..426
                     /locus_tag="VW157_001682"
     CDS             112..426
                     /locus_tag="VW157_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552951.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            1110..1271
                     /locus_tag="VW157_001683"
     CDS             1110..1271
                     /locus_tag="VW157_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552952.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1363..3042
                     /locus_tag="VW157_001684"
     CDS             1363..3042
                     /locus_tag="VW157_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9552953.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRSQKAMEGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGYKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYRVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
     gene            3310..3876
                     /locus_tag="VW157_001685"
     CDS             3310..3876
                     /locus_tag="VW157_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552954.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            4198..4386
                     /locus_tag="VW157_001686"
     CDS             4198..4386
                     /locus_tag="VW157_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9552955.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            4448..5173
                     /locus_tag="VW157_001687"
     CDS             4448..5173
                     /locus_tag="VW157_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9552956.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKK"
     gene            5265..6119
                     /locus_tag="VW157_001688"
     CDS             5265..6119
                     /locus_tag="VW157_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9552957.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            6231..7115
                     /locus_tag="VW157_001689"
     CDS             6231..7115
                     /locus_tag="VW157_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9552958.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            7278..7982
                     /locus_tag="VW157_001690"
     CDS             7278..7982
                     /locus_tag="VW157_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEU9552959.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMNYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDSVAWVRQGIF"
     gene            8132..8413
                     /locus_tag="VW157_001691"
     CDS             8132..8413
                     /locus_tag="VW157_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9552960.1"
                     /translation="MWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
BASE COUNT         3064 a         1141 c         1753 g         2490 t
ORIGIN      
        1 cttcaattat taggcaagct ttaaaaaata ctgaatataa agatcaaata tctagcacag
       61 tattgccaga tatcaaaatt aaattaccga tagacagtag aggtactcca gattggaatt
      121 acatggaaag atatagagat agataaagag agagagggag agatagataa aaagtccacc
      181 cccatttttt atggagaatt caaaaatgta aggttaagca aagaagaata taaaaatcta
      241 aaagaaaaat taaactcaca cacagatata atgataaata aactatccag atacatggaa
      301 agcagtggta agacctatca aaaccactat gtgacaatct taaaatggta tgaagaagat
      361 aaagacaaac taagacagaa aggtttaaat aaaaaaatga attatgatgt aggagaatct
      421 ttatagataa aaagaggtgg aaaggcacta ttaaagactt taagtctaaa aaggtatcaa
      481 agtatgccag agataaaaaa atataaatat gacactttaa aggcgattag aaaattaaag
      541 aaatgctaga agacttttag atgacagtta ccaagagggt ctaattttca ctgctactta
      601 tgcagtggga atttgacctt tttatataag caaattctat ttcaaaattg gttaccaaga
      661 aagacttctt ttcactgtta agtataagag ggtaatttat agaattttgt tacattaaga
      721 taaatatttt ctgatggtca aattttaaaa agattaataa ctctcgataa attttgaacc
      781 ttaacaattg aatagaccaa gacaagacgt taattatttt tggagcgtaa taacctatcc
      841 gtcaagatct ttcggctgaa taattcggca aaaaaagttc tgctaagtaa aaagaaagtg
      901 agcgataagt aagagtttga atatagggag ggatacccta tccgctatga agaaaatgag
      961 gataatgata cttctaaggt tgaagccggc tcatccggaa tagaggcgga ggtgagattc
     1021 ctatgttgct aatccaaagc acttgtcaat gtcaaaaaac ttaattaaac aaataattat
     1081 tagatataaa actttagagg aggtatatat tggacaatga ctggaatgga ttggctgatt
     1141 taatagcaaa tctgattgca aaatatgctg gtgctttaga tttagataat ctacccgatc
     1201 caacgccagc aaaaaatcaa gagatgaaaa atagttttga catggcaaaa acacaaattg
     1261 agacggacta ataaaagtgt tataatatac ttgaaatgat agtccaaact taaattaaaa
     1321 aaattatata tgatatagaa aaattatatt gaggttaata ctatgtcaaa agaaaaaata
     1381 aaagtatacc tctatacacg agtatctacg tcaatacaga tagaggggta ttcgttagag
     1441 gcacaaaaat cacgaatgaa agcttttgct atttacaacg attatgaaat tgttggagag
     1501 tatgaagatg ccgggaagtc tggcaagtct attgaaggta gaatacagtt caatcgaatg
     1561 atggaagata taaaatctgg aaaagatgga gtatcttttg ttcttgtgtt taagttatca
     1621 agatttgcaa gaaatgccgc tgatgttctt tctacacttc agataatgca ggattatgga
     1681 gttaatctta tctgcgttga agatggcatt gattcatcta aagatgctgg gaaactaatg
     1741 atttctgttt tatcagctgt ggctgaaata gaaagagaaa acatccgtat tcaaacaatg
     1801 gaaggtcgca gtcaaaaagc aatggaagga aaatggaatg gtggctttgc tccgtatgga
     1861 tataaacttg aagacggcaa gctgtttata aatgaggaag aggcagttgc aataagaacg
     1921 attttcgacc agtatgtaaa tactacgata ggagccaatg ggatatctaa atacttagag
     1981 aatcatggaa ttagaaaaat cccaagacag aatggtaaga atcctttgtt tgatgcaggt
     2041 cttataagaa agatattaaa gaatcctgta tataatggga aaatagcatt tggaagaaga
     2101 actttagaaa aagttcatgg tacaagaaat gaatataagc aggttgaaca agatgaatat
     2161 ctaatatctg aagggataca tgaagctata gtttccgatg aagtttggca agctgctcag
     2221 gttaagctaa aatctcaagc aaagaaatat gagcatgtga ataaaggaaa agatacacgc
     2281 acacacttgc tttctggaat tgtaaaatgc ccgatatgtg gagtgggaat gtttgggaac
     2341 aagtgtatca agaaaaagaa agatggcaca aagtataaag atttttatta ctatggttat
     2401 aaacataggc agatgataag aggtcataag tgtacattca gtaagcaaat tagagaagaa
     2461 ttgttagatg atgctgttgc agaggtgatt gtcaagatag taagtaatcc gaaatttgct
     2521 tctatgatgc aagaaaaaat caacatgaag gtggatacct ctgaaataga aaaagaaata
     2581 gataattacc aaaaggaatt gagaaagagt cattctacaa agtttaagct aattgaggaa
     2641 atagataatt tagatgttga agataagcac tataagagaa ggaaacagga tttagatgat
     2701 agactatatc gtatgtatga caaaatagat gaattagaat catcactaat tgatgcgaaa
     2761 gcaaagaaac agactattga agctgaaaaa cttacaggag ataacatata tagggttctg
     2821 atctattttg ataagctcta taaagtcatg aatgatgtag agcgtaggca gttaatttca
     2881 gctttgattt ctgaaattca agtttatgaa gaaaaacaat ctaacggaca atggctaaaa
     2941 tccattactt ttaagctacc aattatagaa gaaaatctca acatagattt ggacaatgat
     3001 gaacaagttg aatgtgtaag tttattagag aaacgtagtt aatcctcaaa aggttcccca
     3061 aactttttga gtcccactga cgcatcacgc tgaggcggta tcactgcttg tacgagttga
     3121 agcatcagcg aagtaaaagt agatgttcag ccaatgcgac gaggtgagac cgaaggtcga
     3181 taaggtgtgt tctgggtata ggactcgtaa agtaagtagg aaatatgatg agatgataca
     3241 gagagcctga attggtcagg tgacgtgctt atttacatta gcctgataat gaaaatggag
     3301 agattatcga tggttatatt ggaattgtac caaaatgatt attctaaaga tttagtagca
     3361 tttgattcca tagaagatgg gaaagccttt gtagctcaaa tcccgggata cactctggaa
     3421 acagaagatg gttttgaagt ggagtatttt aatcctaaaa acataccgga ttatatggaa
     3481 attattttta acggaaatat tgttccttta tctaaattta tgttcgatcc tgaggaaaat
     3541 gtaaacatta tttggaaaga gatttcaaat ttatccctca aaaatgatag agtgatcgaa
     3601 ggatattcaa aaattgatgc atatgtcgtt aacaaccatg aagttaaagc ctatgtcgaa
     3661 acaagagaaa cgaactatcg taaagcaaaa gacttcttag aaagccgcgg atatgaaatc
     3721 gacagaagct ttttcggaag tgaagacggt gaagcgattc tttacagaaa aaaagggatc
     3781 gaagtttggc atttcttgtg tcacttagac ccaatgtttg tagagattga agatgtagaa
     3841 ggatatgtta aagaagaagt tggagagatt caatagtcaa ccaaagttcc ttgtgagtat
     3901 atacctttgc tagtaaaaga gtcaaaacgt tataacgaag ggggggaaga aaacccttga
     3961 gtgtaagtct ttttcttgtt tagagggaaa gtattagagt agccgctcat aatttatact
     4021 agatttgata actgatttga gacagatttt atttctacaa atattgtgaa aattttttaa
     4081 taccaaagat ttaaagttac tgtgttaaaa atcctctcca cttttaaact aatgtattat
     4141 aaaagccagt tgacacaagt gttacacctg tgatagaatt aacttgaggt gatgaaaatg
     4201 gcaaccgata aaaatagaat aatgattagc ttggatgata aaaatctaga gaaacttgaa
     4261 aatttagtag aagatgctag agatagaagg ggaatgcgac taacaaagtc ccaggttatt
     4321 gaattactcc taaataccgt tgattatttc gatgatatta tgggggctat ttactcaaaa
     4381 aaataagagc aaaattgcta ttatttttta caaattggtg taacactagt gatataggag
     4441 tgatgaaatg gttgtttata ttagacaaag taaattacca agtgaggttt caattaataa
     4501 atacaatgct caagtaggtg cctacttaca aggagaagaa gttattttat atcaatcttt
     4561 ttctgaaata aaagagttaa caagtgaaga tatagttgta gattatataa tggagactag
     4621 agcattacta aaaatgatgg gcttaaacgt tccggttcat gactatccta ttgagcttaa
     4681 agagttttat ggtcgaaaga tttatgctgg tattttagga gagattgtga atatacctga
     4741 taattgggga aaatttatta agcctaaagc tggttcaaaa gtctttactg gaagagttgt
     4801 taatggaacc catgatttaa taggtattgg tctacctttc gactatccta tatggattag
     4861 tgaggttgta gaattcatag ctgaatggcg ttgttttgtg ttagatggtc gcgtattaga
     4921 tgttcgaccc tatacaggtg attatcatgc acaatttgat gcaagtgtaa ttgatgaagc
     4981 gatatcatgt tggaaagatg cgccaatagc ttatggacta gatatcggtg ttactcgcga
     5041 tggcagaaca cttgttgttg aagtaaatga tggttatgca ttgggaaatt atggcctatc
     5101 tcctttaaaa tcaatcaatt ttcatagagc taggtggaaa gaaatggtaa aaccctattt
     5161 tgaaaaaaaa tgaaattttt aaaatccaac aggatgttgt tttctaaaat aaatatggta
     5221 tataaccaat ataaaaagca tcaaaatgaa caaggaggaa tctgatgctt ggagcaatta
     5281 ttggagatat tgtcggttct gtttacgaat ggaacaatat caaaacaaag gactttcctt
     5341 tatttcggaa ggactgcttt tttacagatg acacggttat gacctgtgct gttgcagaag
     5401 caattatgaa tggtggacaa aaagacgatt tcattgacgc gatgaagaaa tatggcagaa
     5461 tgtatccgaa tgctgattac ggtgctcggt ttaatgcatg gctaaacagc gataaccgtg
     5521 agccttataa tagctttggg aatggatcag ctatgcgtat ttctccatgt gcttggatca
     5581 tggactgtgg tttttatgcg aaaactggta tgtggccatc atctagagga cttacgagtc
     5641 tttctgcaga ggtaactcat aatcatccag aaggtgtcaa aggcgctatg gctacagctg
     5701 atgctatctt tctatgtcgt ttttactttg gtggttattg tagagagtac gagcaatcaa
     5761 ttaacgacaa tcctacagag tgtaaaagac gaattaagga ttatatagaa aaagaatacg
     5821 attacaatct atctcaaact ttagatgaaa tccgtcctaa ctatcgtttt aacgaaacat
     5881 gtcaggaaac tgtccctcaa gccattatcg cctttcttga aagtagagac ttcgaagatg
     5941 cgataagaaa tgccatctca cttggtggcg acagtgatac tctcgcagca atcacttgta
     6001 gcatagcaga ggcagcttac ggtattcctg attggatcaa ggataaggcc tattcttact
     6061 tggatgaacc cttaaaggat gtagttaggc gatgggaaaa tagaataaaa gcgtattaat
     6121 ctccagtacg tcagatttct cttttctgta ttttcattgt ataataggat tacatttact
     6181 tgcatacgac gaaatgtaaa catttctgtt taaaattaag gataaaatca atgaaaaaag
     6241 caatggtaat tatcaaccct acttctggtg gcgagaaggc tttggattac aaagaaaagc
     6301 tggaaaataa agcaaaagaa tactttgaat atgttgaaac caaaattacc gaaaaagcgc
     6361 tggatgcaac acattttgct gaagaagctt ctcgtgagca gtatgatgca gtggttgtgt
     6421 ttggtggaga tgggactgtc aatgaagtca tttcaggtat tgatgagaga gactacattc
     6481 ctaagttagg gattatccca ggcggtacgg gtaacctcat tacaaaactt ttggaaatca
     6541 atcaagacat cgatggcgca attgacgaac tggattttga tttaaccaat aagattgata
     6601 tcggtaaagc aaatgacaac tattttggtt atatctttag tatcggttct ctgcctgagg
     6661 cgattcacaa tgttgaaata gaggacaaaa caaaattcgg tattttaacc tatgctgtaa
     6721 ataccatgaa gtctgtcatg acagatcagg tctttaacat taaggttgag acagaaaatg
     6781 gaaattatgt tggtgaagct agccatgttt tggtcctttt gacaaattac ttcgctgata
     6841 agaaaatctt tgaagaaaac aaggacggct atgccaacat tttgattctg aaagatgcct
     6901 ctatattctc caaattatcc gtcattcctg atttattaaa aggggatgtt gtcgcaaatg
     6961 ataatatcga gtatatcaaa gcgcgtaata ttaaaatctc ttcagatagt gaattggagt
     7021 cagatgttga cggagataaa tcagataacc tacctgtaga aatcaaagtc ctagctcagc
     7081 gagtagaagt attttcaaaa ccgaaagagg attagtatat agagaaagcc ttttttaagg
     7141 ctttttgtat actttaaaag atagttcctt taacaacgga cattccttgc aaatagttta
     7201 caaaaatagt atactggatt tattgagttt gaaaacgttt gcgtaaaatt tgaatgaata
     7261 ctttaggaga caaattgatg gaattgagtg ctatttacca taggcctgag tcggagtatg
     7321 cctatcttta taaggataag aaactccata ttcgaattcg aactaagaaa ggggacattg
     7381 aaagcatcaa cttgcactat ggggaccctt ttatctttat ggaggagttt tatcaggata
     7441 caaaagaaat ggtcaagata acttctggta ccttatttga ccattggcag gttgaagtgt
     7501 cagttgactt tgcacgtatc cagtatctct ttgagctcag agatacagaa ggtcaaaata
     7561 ttttgtatgg cgataaaggg tgtgtggaaa attctctaga aaatcttcat gcaattggga
     7621 atggatttaa gttgccttat cttcatgaga ttgatgcctg caaagttcct gactgggttt
     7681 caaatacggt atggtatcag atatttcctg aaagatttgc caatggcaat gctctattaa
     7741 accctgaagg gactttagac tgggattcat ctgtcacacc taagagcgat gatttctttg
     7801 gtggtgattt acaggggatt attgatcata tgaattactt gcaagacttg ggtattactg
     7861 gactatatct ttgtcccatc tttgaatcta caagcaatca caagtacaat acgacagatt
     7921 actttgaaat tgaccgtcat tttggagaca gtgttgcatg ggtgcggcaa gggatttttt
     7981 agaactaatt aatcacaaag ggctacctct ttttaatccg cttgtaggtg attctcatgt
     8041 aaataataga caagaatatg acaatacagg gagtggaaat ttataacctg aaaagtggaa
     8101 tgaaactgca aagcagcttt ataatgctat aatgtggttg attattttat ggaatgctaa
     8161 gccggataca cctttattta attttaaaga cgaagtaatt aagtataaaa catatgagcc
     8221 ttttgaaagc agtataaaaa gagtaaatac tactataaag aatggtagta aagggaaaac
     8281 tctgactgag atgattaatg gctacagagc ggataacgat attagagatg aaatttgtaa
     8341 ctttaatatt ctgaaaaata aaattcgtga tatgaaagac caacaaggaa atacaatgga
     8401 atcttacttt tagttattgt tgaattttgg gtattctata aaatatcc
//
LOCUS       DAVKJD010000075         8359 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKJD010000075 DAVKJD010000000
VERSION     DAVKJD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.075"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            96..329
                     /locus_tag="VW157_001692"
     CDS             96..329
                     /locus_tag="VW157_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9552961.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            329..1663
                     /locus_tag="VW157_001693"
     CDS             329..1663
                     /locus_tag="VW157_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9552962.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKDDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            1673..1927
                     /locus_tag="VW157_001694"
     CDS             1673..1927
                     /locus_tag="VW157_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9552963.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            2023..2418
                     /locus_tag="VW157_001695"
     CDS             2023..2418
                     /locus_tag="VW157_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9552964.1"
                     /translation="MSKKLNRKKQLRNGLRCSGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            2831..3280
                     /locus_tag="VW157_001696"
     CDS             2831..3280
                     /locus_tag="VW157_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU9552965.1"
                     /translation="MIGVVARENAAEQIKQYQKFPVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVVPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            3267..3827
                     /locus_tag="VW157_001697"
     CDS             3267..3827
                     /locus_tag="VW157_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9552966.1"
                     /translation="MARTELPDKIETECLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRYGDDVLELGY
                     TLHSDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            3827..4405
                     /locus_tag="VW157_001698"
     CDS             3827..4405
                     /locus_tag="VW157_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9552967.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQHQPNELNH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            4398..7049
                     /locus_tag="VW157_001699"
     CDS             4398..7049
                     /locus_tag="VW157_001699"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9552968.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPVFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELSRLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ECDKQADYQAKYDATVARIDEMKKLVK"
     gene            7130..7294
                     /locus_tag="VW157_001700"
     CDS             7130..7294
                     /locus_tag="VW157_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9552969.1"
                     /translation="MGKIVAIDLFSGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDME"
     gene            7345..>8359
                     /locus_tag="VW157_001701"
     CDS             7345..>8359
                     /locus_tag="VW157_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9552970.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNIKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKISTQRSKQDFIRLRSKPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEII
                     SEIMDESYTDIQILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVND
                     IVNAQSNSLILIDEPEISLHPSAIYKFKEFLLQECLNKKHQIIITTHSTQLIKDFPRE
                     AVKLLVKNGEKVDVIENIDYQDAFFELGDVYHSRKMIYVEDRLAKYILEFVITHSGSE
                     NLKQ"
BASE COUNT         2551 a         1588 c         1885 g         2335 t
ORIGIN      
        1 acaacctcaa agcactgttt tgagcaacct gcggctagct tcctagtttg ctctttgatt
       61 ttcattgagt attagaacat acaatggagg tcgtcatgga caatatcatc gatgtgtcaa
      121 ttcctgttgc agaagtggtg gacaagcatc cagaagtctt ggaaattcta gtggagttgg
      181 gttttaaacc ccttgccaat cccttaatgc gcaatacagt tggtcgtaaa gtatcactta
      241 aacagggttc taagctagca ggaactccta tggacaagat tgtacgcaca ctggaagcga
      301 atggctacga agtgattgga ttagactaat gacagatgaa cggattcata tcctacggga
      361 tattttgtta gaattgcaca atggcgcctc tcctgagtcg gttcaagatc gctttgatgc
      421 gacctttacg ggcgtgtcag ccatcgagat ttcccttatg gagcacgagc tgatgaactc
      481 ggattcgggc gtcacttttg aagatgttat ggaactctgt gatgtccatg ccaatctttt
      541 taaaaatgct atcaaaggtg tcgaagtttc agatactgag catccaggtc acccagttcg
      601 tgtcttcaaa gaagaaaatc tggctctccg tgcggccttg attcgcattc gtagattgtt
      661 agatacctat gagtctatgg aagacgagga aatgctggcg gagatgcgta agggtttggt
      721 gcgtcagatg ggacttgtgg gtcaatttga catccattac caacgtaagg aagaactctt
      781 ctttcctatc atggagcgct atggacacga ttcacctccc aaagttatgt ggggagtgga
      841 tgatcagatt agggaactct ttcaaacagc tctaacgaca gccaagtcac taccagaagt
      901 gtcaattagc agtgtaaagg aagcttttga agcttttgcg acagagtttg aaagtatgat
      961 tttcaaggaa gagtccatcc tcctcatgat tctccttgag tcttttactc aggatgactg
     1021 gcttcagatt gcggaggaga gcgatgccta tggctatgcc atcatccgtc cgtcagagaa
     1081 atgggtgcca gaacgacaga gctttattga ggaaaagatt gcagaggagc ctgtacagct
     1141 agatacggca gaaggtcaag ttcaacaagt catagatacg ccagaaggcc attttaccat
     1201 tacctttacc cctaaggaaa aggaagctgt gctggaccgc catagtcaac aggcttttgg
     1261 taatggctat ctttcagtcg agcaggccaa tctcatcctc aatcacctcc ctatggagat
     1321 tacctttgtc aataaagatg atattttcca gtattacaat gacaatacgc cagctgatga
     1381 gatgattttc aaacggacgc cgtctcaagt cgggcgcaat gtcgaactct gccatccgcc
     1441 taagtacttg gacaaggtca aaactatcat gaaggggctt cgtgagggga ccaaggacaa
     1501 gtatgaaatg tggttcaagt ctgagtcgcg aggtaagttt gtccacatca cctatgctgc
     1561 agtacacgat gaagacggag aattccaagg agtgttggag tatgttcagg atatccagcc
     1621 ctaccgtgag attgatacgg actattttcg tggattagaa taaggagaaa aaatgagtta
     1681 cgaacaagaa tttatgaagg aatttgaagc ttgggtcaat acccaaatca tgattaacga
     1741 catggcgcac aaggaaagtc aaaaagttta cgaagaagac caggacgagc gtgccaaaga
     1801 tgccatgatt cgctacgaga gtcgcttgga tgcttatcag ttcttgcttg gtaagtttga
     1861 aaacttcaaa gtaggcaagg gattccatga tttgccagaa ggcttgtttg gtgagcgaaa
     1921 ttattaaacg agaaagattc ttgatttttc actaaaatct tgatagaatg tttatgttaa
     1981 atccttgtca gagcagggat tttttattga aaggatttta tcatgtcaaa gaaactcaat
     2041 cgtaaaaaac aattacgaaa tggcctccgt tgctcaggtg ccttttcaag tactgtgact
     2101 aaggttgtag atgagacaaa aaaagtcgtg aagcgtgcag aacagtcagc aagcgcagct
     2161 ggtaaggctg tttctaaaaa agttgaacaa gcagtagaag ctaccaaaga gcaagctcaa
     2221 aaagtagcta attctgtaga agattttgca gcaaacttgg gtggacttcc acttgatcgt
     2281 gccaagactt tctatgatga aggaatcaag tctgcttcag atttcaaaaa ctggactgaa
     2341 aaagaactcc ttgacttgaa aggaatcggc ccagctacca tcaagaaatt gaaagaaaat
     2401 ggcatcaagt tcaagtaatt tttcttgagc cttgcatttc cgagaaaaac ttgctacaat
     2461 agagccatta gaggtgtttt gaatcccaca ttttacagaa agtggcggcg ctgagaagtc
     2521 cacaaatgtg tcaaaactgg ttgctaatgg atgaaaaatt gaaataaaag tgtctttttg
     2581 ctttaaagac gagagttgcg ggcaactgcc cgaaattggg tggtaccgcg gataaacaca
     2641 ttcgtccctg tcatgtagat ggcagggctt ttcttttacg tcttagtcaa agggggttgt
     2701 tatgaagcaa tcttttaaca caagtaaact atattatggt tttcctgttt ttattttagg
     2761 atatcaagat tagaattttg gctataatat cacgacctgt agttcctctt atagtctagg
     2821 agattgggtt gtgattggag tcgttgcgag agagaatgcc gcagagcaga tcaaacagta
     2881 tcaaaaattt cctgtgaata tttctgatga aacttctatg cttgcgatgg agcaggctgg
     2941 ttttatcagt catcaggaga aattggaacg tttgggagtg cattatgaaa tttctgaacg
     3001 aactcagact cctattttag acgcctgtcc acttgtttta gattgtcggg tagataggat
     3061 tgttgaggaa gacggtattt gccacatctt tgccaagatt cttgagcgac ttgttgttcc
     3121 agaactcctg gatgaaaagg gacattttaa aaatcaactg tttgccccaa cctatttcat
     3181 gggagatgga tatcagcgcg tttatcgcta tctggacaag cgtgtagata tgaagggcag
     3241 tttcatcaaa aaagcgagga agaaggatgg caagaactga gctaccagat aaaatcgaaa
     3301 cagaatgtct cgttttacga gtccgtactg tggcggatgc tgaggatatc tttgactatg
     3361 ctagtttgcc agaggtcgcc tatccagcag gttttcctcc agtcaagacc ttggaagatg
     3421 agatttatta tctggagcac attcttccgg agcgtaatca aaaggaaaat ctcccagcag
     3481 gctacgggat tgtcgtcaaa ggaacggata agatcgttgg ctctgtcgat ttcaaccacc
     3541 gctatgggga cgatgtgcta gagcttggct ataccttaca ctcagactac tggggtcgag
     3601 gttatgttcc agaagctgcg cgtgccttga ttgacttagc ctttaaagat ttgggtcttc
     3661 acaagattga actaacttgc tttggatata accttcaaag taaacgagtc gcggaaaagc
     3721 ttggctttac cctcgaagct cgcataagag accgcaaaga tgctcaaggc aactgctgtg
     3781 atgatttaag atatgccttg ctgaagagtg aatgggaggt gatttgatgc atcttttcat
     3841 cattggtgct ccagcatcag gaaaaatgac cattggtcaa gaattatctc gactgacgga
     3901 tgctacccta ttttataacc atcaagccat cgattttgca ctagaaatct atcaggacta
     3961 tacagaggaa atgtgggaat ttgttcgtgg aatcactttt tcattccttg gagcgagtgc
     4021 aagaaatcag cgatcagtaa ttttaacaga tgtaattgat ttttcaaatc agtatcatct
     4081 gatatattta aagcaaattc agagtttgtt ggatgattat catcaagaga ttctttttgt
     4141 tgagttggaa acaagtcttg aagagtgcat acgtcgaaat cgaacggaga atcgattaaa
     4201 gtacaaaccc ttaaaacgac atattgaggt atctgaaaga gaaattatag agaccgctga
     4261 aacacttcaa ttaaattccc aacatcaacc gaatgagttg aaccactact ttaaaataaa
     4321 taatacgaat ttgtctgcag aagaagctgc taagcagatt caaaataaaa taaacaaaat
     4381 agagaaagga cacacacatg tctaaagaac tttcacctaa atacaatcca gccgaggttg
     4441 aggctggtcg ttaccaaaaa tggcttgatg ccgatgtttt caagccttca ggcgatcaaa
     4501 aggctaagcc ttattcaatc gtgattccac caccaaacgt aactgggaaa ctccaccttg
     4561 gtcacgcttg ggatacaact ttgcaagata ttatcatccg tcaaaaacgc atgcaaggtt
     4621 ttgataccct ttggcttcct gggatggacc acgcagggat tgccactcag gctaaggttg
     4681 aggagcgctt gcgtggtgag ggcatttccc gctatgacct tggtcgtgag tctttcttga
     4741 cgaaagtctg ggaatggaaa gacgaatatg ccactaccat caaggaacaa tggggcaaga
     4801 tggggctctc tgtagactat tctcgtgagc gtttcactct tgacgaaggt ttgtcaaaag
     4861 ctgttcgtaa ggtctttgtg aacctttaca agaaaggctg gatctaccgt ggtgagttta
     4921 tcatcaactg ggacccagca gctcgcacag ccctttctga tattgaggtg attcacaagg
     4981 atgtggaagg tgccttctac cacatgaatt acatgctgga agatggttca cgcgcccttg
     5041 aagttgctac aactcgtcct gagactatgt ttggggacgt tgcggttgcg gttaatccag
     5101 aagacccgcg ctacaaggac ttgattggta aaaacgtcat ccttccaatc gctaataaac
     5161 tcatcccaat cgttggagat gagcacgcag atcctgagtt tggtactggt gtcgtgaaaa
     5221 tcacacctgc ccacgatcca aatgacttct tggttggcca acgtcataac ttgccacaag
     5281 tcaacgtcat gaacgacgac ggaactatga atgagcttgc ctttgaattt tcaggcatgg
     5341 atcgttttga agctcgtaag gcagtcgttg ctaagttgga agaaatcggt gcccttgtca
     5401 aaatcgaaaa acgtgtccac agtgttggtc actcagaacg tacaggtgtc gtagttgagc
     5461 cacgcttgtc tactcaatgg ttcgtcaaga tggaccaatt ggctaagaac gccattgcca
     5521 accaagacac agaggacaag gtcgaattct acccacctcg tttcaacgat accttccttc
     5581 aatggatgga aaatgtccac gactgggtta tctctcgtca gctttggtgg ggtcaccaaa
     5641 tccctgcctg gtacaatgct gatggtgaaa tgtatgtcgg cgaagaagct ccagaaggtg
     5701 acggatggac tcaggacgaa gacgtcttgg atacttggtt cagttctgcc ctctggccat
     5761 tttcaaccat gggctggcct gaagtcgact cagaagactt taaacgttat ttcccaactt
     5821 caaccttggt aacaggttac gacatcatct tcttctgggt gtctcgtatg atcttccagt
     5881 cattggaatt cacaggccgt caaccattcc aaaacgtcct tatccacggt cttattcgtg
     5941 acgagcaagg acgcaagatg tctaagtctc tcggtaacgg gattgaccca atggatgtta
     6001 tcgagaaata cggtgccgat gcccttcgat ggttcctttc aaacggttct gcgccaggac
     6061 aagacgtgcg tttctcttat gagaaaatgg atgcttcatg gaactttatt aacaagattt
     6121 ggaacatttc tcgctatatc ctcatgaaca atggaggttt gacgctggat gtggcgcatg
     6181 acaatgtcac aaaagttgca acaggtgagg ctggtaatgt gacggaccgc tggattctcc
     6241 acaatctcaa cgaaaccatt gcaaaagtta ctgaaaactt tgataagttc gagtttggtg
     6301 tggctggtca tatcctttac aacttcattt gggaagaatt tgccaactgg tatgttgagt
     6361 tgaccaagga agttctttac agcgacaatg aagacgataa ggtcattact cgatctgttc
     6421 tcctctatac gctggataaa atccttcgtc tccttcactc aataatgccg ttcgtgacag
     6481 aagaaatctt tggtcagtac gctgaaggct ctatcgtgac agcagcatac ccaactgtca
     6541 acccagtctt tgaggacctt gctgcccaca ctggtgtgga aagtctcaaa gacttgatcc
     6601 gtgccgttcg taatgcgcgt gcggaagtaa atgtagcacc aagcaaacct atcacaatcc
     6661 ttgttaagac tagcgatagt gacttggaag ccttctttaa cagcaatgtc aactacatca
     6721 aacgctttac aaatccagaa catttggaaa tcgcatcaac catccctgca cctgaactgg
     6781 ctatgtcaag tgtcatcaca ggagcagaaa tctacctgcc actggccgat ctcctcaatg
     6841 ttgaagaaga actgtcccgt ctcgacaagg aacttgctaa atggcaaaaa gaactggaca
     6901 tggttggtaa gaaactctct aacgaacgct tcgtagccaa cgccaaacca gaagtcgtcc
     6961 aaaaagaatg cgacaaacaa gctgactacc aagctaaata tgacgcgacc gtcgcacgta
     7021 ttgatgagat gaagaagttg gtgaaatgat gttaaaaacc taaccctctc gggttgggtt
     7081 ttacatttga taatgttata ataaaaagga gaaactttag gagaatacta tggggaaaat
     7141 tgttgctatt gatttattta gcggtgcagg tggcactaca agtggcctta aaaaatcagg
     7201 catagatgtt caagttgctg ttgaaattga ttccgtagct gttaaaactt ataagttaaa
     7261 taatcctgag gtgtctgtaa tagatatgga gtaaataaaa cttcaatttt acaagcttta
     7321 tatggttctc cagccaataa aagtgtgtct gaatattggt ttagtacaaa tgttgatcaa
     7381 atagatgaag tggatgggaa acaatgttta atctattcat attataacat taaagcttct
     7441 agaaatgtag aagttcttaa aggccgttct ggaacaaaaa aaggattaga ttattgggaa
     7501 ccatatgctc ctcaaaaaca atatgagatg gaaagattac ccaaaaataa atatattgga
     7561 agttcttcga cagacagatg ggatggcatt gaaaaaaatg ttgtcttttg tgattgtaaa
     7621 gaatatgttt ctgcttttga tttatttttc tatcactata attttaaaaa aatttctact
     7681 caacgtagta aacaagattt tataagatta cgatctaaac cagttgctga tattttaaag
     7741 aataatacaa gtagctatac gcgttataaa aaggaaatgg ttatagataa tgttaaagtt
     7801 gatgataaag tttgtgaaat tattagtgaa ataatggatg aatcttatac agacattcaa
     7861 attttaacac ataagttgta ttcaaaaggt gatgatataa aagcttccaa aactatttgg
     7921 atgaaaaaat ctggaaaaga atattccgag gcttttgcag gaacaggtga ggctagaatt
     7981 attttgttag taaatgatat agtaaatgcg caatcaaatt cattaatatt aatagatgaa
     8041 cctgaaatct cacttcatcc gagtgcaatc tataaattta aagagttttt acttcaagag
     8101 tgtttaaata aaaaacatca aattattatc actacacatt ctacacaact tataaaagat
     8161 tttcctagag aagccgtgaa acttttagtg aaaaacggag aaaaggtaga tgttattgaa
     8221 aatattgatt atcaggatgc attttttgaa ttaggtgatg tgtatcattc taggaagatg
     8281 atttatgttg aagatagact agctaaatat attctagagt ttgttatcac tcattcaggt
     8341 agtgagaatc ttaaacaga
//
LOCUS       DAVKJD010000076         7809 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKJD010000076 DAVKJD010000000
VERSION     DAVKJD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.076"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            81..770
                     /gene="coaB"
                     /locus_tag="VW157_001702"
     CDS             81..770
                     /gene="coaB"
                     /locus_tag="VW157_001702"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9552971.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            782..1333
                     /gene="coaC"
                     /locus_tag="VW157_001703"
     CDS             782..1333
                     /gene="coaC"
                     /locus_tag="VW157_001703"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9552972.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            1317..1880
                     /locus_tag="VW157_001704"
     CDS             1317..1880
                     /locus_tag="VW157_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9552973.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            2162..2689
                     /locus_tag="VW157_001705"
     CDS             2162..2689
                     /locus_tag="VW157_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9552974.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAIFVILASSFGFLMAGYPMVIVFRAFSHIFFGTLGALYLQKFPDTL
                     DKPKASWIFNFVLALVHALAEVLACVVFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            2701..3216
                     /locus_tag="VW157_001706"
     CDS             2701..3216
                     /locus_tag="VW157_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9552975.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYVYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGRVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFKPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(3213..3680)
                     /locus_tag="VW157_001707"
     CDS             complement(3213..3680)
                     /locus_tag="VW157_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9552976.1"
                     /translation="MSRAQLTILTNICLIEDLEAQRVVMQYRAPETNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEASDKSEFFYPRRTEDDWEKKIF"
     gene            complement(3753..4241)
                     /locus_tag="VW157_001708"
     CDS             complement(3753..4241)
                     /locus_tag="VW157_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9552977.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKTALTALNRFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKMLELGKPIKEKRMVFY
                     SK"
     gene            complement(4207..4770)
                     /locus_tag="VW157_001709"
     CDS             complement(4207..4770)
                     /locus_tag="VW157_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9552978.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(4780..6768)
                     /gene="uvrB"
                     /locus_tag="VW157_001710"
     CDS             complement(4780..6768)
                     /gene="uvrB"
                     /locus_tag="VW157_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9552979.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(6845..7798)
                     /locus_tag="VW157_001711"
     CDS             complement(6845..7798)
                     /locus_tag="VW157_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9552980.1"
                     /translation="MKNKRIFKDFQASKMNLNIYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
BASE COUNT         2339 a         1745 c         1372 g         2353 t
ORIGIN      
        1 aactataata aattttcgtt tttaccaaaa atttatcact ttcattttac ttaccgctta
       61 tttttgtgta caatagtgct atgaaaattt tagttacatc gggcggtacc agtgaagcta
      121 tcgatagcgt ccgctctatc actaaccatt ctacaggtca cttggggaaa attatcacag
      181 agactttgct ttttgcaggg catgaagttt gtttaattac gacaaaacga gctctgaagc
      241 cagagcctca tcctaaccta agtattcgag aaattaccaa taccaaggac cttctaatag
      301 aaatgcaaga acgtgttcag gattatcagg tcttgatcca ctcaatggct gtttctgact
      361 acactcctgt ttatatgaca gggcttgagg aagttcaggc tagctccaat ctaaaagaat
      421 ttttaagcaa gcagaatcat caggccaaga tttcttcaac tgatgaggtt caggttttgt
      481 tccttaaaaa gacacccaaa atcatatccc tagtcaagga atggaatcct actattcatc
      541 tgattggttt caaactgctg gttgatgtta ccgaagatca tctggttgac attgcacgaa
      601 aaagtcttat caagaatcaa gcagatttaa tcatcgcgaa tgacctgact caaatttcag
      661 caggtcagca ccgagctata tttgttgaga aaaatcagct tcaaacagtc cagactaaag
      721 aagaaattgc agaactcctc cttgaaaaaa ttcaagccta tcattcttag aaaggaaaac
      781 tatggcaaac attctcttgg ctgtaacggg ttcaatcgcc tcttataagt cggcagattt
      841 agtcagttct ctaaaaaaac aaggccatca agtcactgtc ttaatgactc aggctgctac
      901 agagtttatc caacctttga cactacaggt actctcacag aatcctgtcc acttggatgt
      961 catgaaagaa ccctatcctg atcaggtcaa tcatatcgaa cttggaaaaa aagcagattt
     1021 atttatcgtg gtacctgcaa ctgctaacac tattgcaaaa ctagctcacg gatttgcgga
     1081 caacatggta accagtacag ctctagccct accaagtcat attcccaaac taatagctcc
     1141 tgctatgaat acaaaaatgt atgaccatcc agtaactcag aataatctga aaacattaga
     1201 aacctacggc tatcagctga ttgctcctaa ggaatcccta ctagcttgtg gagaccacgg
     1261 acgaggagct ttagctgacc tcacaattat tttagaaaga ataaaggaaa ctatcgatga
     1321 aaaaacgctc taatattgca cccattgcta tcttttttgc taccatgctc gtgatacact
     1381 ttctgagctc acttatcttt aacctttttc catttccaat caaaccgacc attgttcata
     1441 ttcctgtcat tattgccagc attatttatg gtccacgagt tggggttaca cttggatttt
     1501 tgatgggatt acttagcttg acggttaaca cgattacgat tctaccgaca agctacctct
     1561 tctctccctt cgtaccaaac ggaaacatct actcagctat cattgccatt gtcccacgta
     1621 ttttgattgg tttaactcct tacttagtct ataaactgat gaaaaacaag actggtctga
     1681 ttttagctgg ggctcttggt tccttgacaa atactatctt tgtccttgga ggaatcttct
     1741 tcctatttgg aaatgtttat aatggaaata tccaacttct tctggcaacc gttatctcaa
     1801 caaattcaat tgctgaattg gtcatttctg caattctaac cctagccatt gttccacgac
     1861 tacaaacctt gaaaaaataa aaacaatctc cgctttcaaa cctgaaggtg gagattttgc
     1921 tctatacagt ttctttttaa tgaaatcttt actatagtaa aacgaaataa gaacaggata
     1981 aatcgatcag gacagccaaa tcgatttcta acaatgtttt agaagtagag gtggactatt
     2041 ctagtttcaa tctactataa cttttaacta aaaaatgatc tataaaccca agtaaattct
     2101 tgctttattg tctggtttat tgtaccatac tagtgtatat acagttaaaa aggagattct
     2161 tatgaacaca cggaaaaaga cacaatttat gacaatgaca gccctcttaa cggctattgc
     2221 gattttgatt ccaattgtta tgccctttaa gattgtcatt cctcctgctt cttacacttt
     2281 ggggagccac atcgctattt ttatcgccat gttcttgtca cccttgatgg caatttttgt
     2341 catcctagcc tctagttttg gatttttgat ggctggctat cccatggtta tagtatttcg
     2401 agctttttcc catatctttt ttgggacttt gggggctctt tacctacaaa aattccccga
     2461 taccttagat aaaccaaaag cctcctggat tttcaacttt gtgttagcgc ttgtccatgc
     2521 ccttgctgaa gtattggcct gtgtcgtttt ttacgcaact tctggtacca atgtagaaaa
     2581 tatgttttat gttctatttg tgctagttgg atttggcaca attatccata gtatggtaga
     2641 ctatacatta gcactagctg tctataaagt gcttcgaaaa cgccgttaaa aggaaaagct
     2701 atgacaaaag atcgcaaaca agctctgctc caactcttga aagaagctcc taaagccctt
     2761 aatggccaaa gtttggcgga acattttcat gtcacacgtc aggtcattgt gcaggacatt
     2821 gcaattttga gagccgatgg cgctcctatc ctatccacca atcgtggcta cgtctataag
     2881 caaattgata ccaatcctta tgtccataaa cttttcaagg tgaaacatga agttgaagaa
     2941 atcggtcaag aactccttgc tatcgttgat aatggtggac gtgttcaaaa taccttgatt
     3001 gaccatcccg tttatggaga aatcgaaacc ttgctgaaac tgtcttgtcg ccgagacgtg
     3061 caacattttc tagaacaagt cgagcattct gattttaaac ctttatctga attgacagat
     3121 ggtgtccatt accacctagt cgaagccgaa acacaacaag acctccacta tatcgaggag
     3181 gccttggatc agctaggtta tttagtaaag gattagaaaa ttttcttttc ccaatcgtct
     3241 tctgtacggc gagggtagaa aaattcagat ttgtccgatg cttccatcat ctccatcaag
     3301 ggtagcatat cataggccag atccaaattt ggaatctggt ctttttgcac ccaagaaact
     3361 tctccttcat ctgaagagcg aagggtacca gagaactcag ttgccttata acaaacgaca
     3421 atatagcgcc cacctgtatc tagtggccaa tttttaatgc cgacaagttg aggattttgg
     3481 atagtcaacc ctgtttcttc atagatttca cgaatgacag actctgcgaa agattcgcca
     3541 ttttctacat ggcctcctgg aaaggcataa ccggaccagc gattagtttc aggagcgcga
     3601 tactgcatca ccacgcgctg agcttcgaga tcttcaatca gacaaatatt tgttaaaatc
     3661 gttaattgtg ctcgagacat aaatttactc actttctaat ttattaaatt tttagacttg
     3721 gtaccagtac cattagcttc tactttttaa aattatttag aatagaatac catccgtttt
     3781 tcctttatgg gctttccaag ttcaagcatt ttttcttgac catcaaaagt aaaacccatt
     3841 ttttgataga aagcaatggc tcgcttgtta tctttcaata cccataagaa aatttcagaa
     3901 aaacgattca gagcagtcaa agctgttttc attaactttt gtgctatgcc ttttccataa
     3961 tagtctttta aaacatataa agcaataatt tcaccagctt gaatagtctc atcacgaaag
     4021 ttaccataac tgataaaacc aactaccttc agatcaccta tcgcaatcaa tgtattttct
     4081 ggatactttt gactaaagaa tcgacatctt tctaatgtca ttgtctcctg aaattctgca
     4141 ggcaaaaggt catcataagc ctctctccac gtttgccagt gaacgaggga tttaccttct
     4201 atctcttcag gagtttccat tgatttgata ataaccgtca tttattttct cccagtcttc
     4261 tctcaaaata ccatatttaa tactatcaaa atatttacct tgataataac gaacttttgg
     4321 aatatgagct tcttttttca ttcttaattt ttcagcaagt ttcatcatac caagatttcc
     4381 tgaccaagtt gtcaaaccca gatgctccaa ctccaagtaa tcctgaaacg tcctatctat
     4441 ccactgcaac atagcaactt tcccaatacc agtgttccag aattttttat cataaatacc
     4501 aattcccaat tccatccatc ttgtttcttt acatacccaa taacgcgaaa cagtcccaac
     4561 tagtttatca tcaacaaaaa taccaaggcg atttgagttg cttaaaatgg attctgattt
     4621 ttgtagttcg aattctttaa aatttgaaaa atactgataa tcatcataat agggagcatc
     4681 atactgtttc caaactggat taagctgtga ataagccatt tcccataaag acatcaaatc
     4741 ttctttcttt aactttctta aataaatcat actattcccc taatccaagg ccttgacttc
     4801 caacatcata tcacggatct gagccgccag ttcaaagtca agcacttcga cggcttcttg
     4861 catttgtttt tctagtttct tgacgagttc tttgcgctct tgtttgttga ggctattgat
     4921 gtcgacttcc ttgtcctctt ccttagcaac tgccttggtc acggcaatca ggtcacggat
     4981 ttctttcttg attgtctgtg gtacgatacc atgctcttca ttataggcca tctgaatttt
     5041 ccgacggcgg gcagtttcat cgatagcacg ttgcatagac tgggtaaccg tatctgcata
     5101 catgatgaca tgaccttcac tgttacgggc ggcacgccca atggtctgga tgagtccacg
     5161 ctcgttacga aggaaacctt ccttgtcagc atcgagaatg gcaaccaagc taacctcagg
     5221 aacgtcaatc ccttcacgaa ggagattgat tccaaccaag acatcaaaga cacccaagcg
     5281 gagatcacgg ataatctccg tccgttccaa ggtcttgata tccgagtgca tgtacttgac
     5341 cttgatgccc atttccttga aatagtcggt caagtcctct gccattttct tggtcaaggt
     5401 ggtgataaag gttcgttcat tcttttcaac acgggcattg atttcaccta agagatcatc
     5461 aatctgtccc atagtcggac ggacttccac ctcaggatcc aaaagccctg tcggtcgaat
     5521 gatttgctca atcaccgtct cagtctgttc attttcatag tcaccaggtg tcgctgatac
     5581 gtaaacaatc tgatgcacat ggctttcaaa ctcctcacga cggagaggac gattgtccaa
     5641 ggcagacggt aaacggaaac cataatttac tagcatttcc ttacgcgaac ggtctccatt
     5701 gtacatgcct tttatttgtc ccatagtcat atgactctcg tcaatcataa tcaaaaaatc
     5761 atctgggaaa aagtcgagaa gcgtataagg aggctcacct tcactacgtc catccatgtg
     5821 gcgagaataa ttttcaaccc catttgtata gcccatctcg cgcagcattt cgatatcgta
     5881 ctctgtccgt tgtttcaagc gctgggcttc aagcaattta ccttccttct caaagacagc
     5941 taactgctct tctaattctg cttgaatctt ggcaacggca acttccatat ggtcgtcatt
     6001 ggtcacaaag tgagtagctg ggaaaatcgc caaatgatcc acctctccca atacctgacc
     6061 tgtcagagcc tcaacctcac ggatacggtc aatttcgtct ccaaaaaatt ctactcgaaa
     6121 ggcatgttca tctcgggaag ctgggaaaat ctctaccaca tccccacgaa cgcgaaatct
     6181 tccgcgttgg aaatcaatat cattacgttc aaactgaata tcgaccaagt cattcaagag
     6241 tttatcacga gaaatctcta gaccaggacg gagactaacg acactatcag cgtattcctt
     6301 gggcgaaccc aaaccataga tacaagagac tgaggccacg acaataacat cattacgctc
     6361 caaaagggct gaggtagctg agtggcgaag cttgtcaatc tcgtcattga cagaactatc
     6421 cttctcaata taggtatcgc tagaagggac ataggcctct ggctggtaat aatcatagta
     6481 ggatacgaaa tactcaactg cattttcagg gaaaaattcc ttaaactccc catagagctg
     6541 accagccaga gttttattgt gggcaataac cagagttggt ttattgactt tagaaatgac
     6601 ctgactcata gtataggtct tccctgttcc agtcgccccc atcagaatct gagctttttc
     6661 tcccccctca atgttatcca ccaactgctc gatagcttgg ggttgatctc ctgatggttg
     6721 atattttgat actagtttaa attgattatc tgtaatgtga ttgatcataa gaatcctctc
     6781 tccatgtgct attcctattt tagcatactt atagtatttt cacgaagaaa aggacggacc
     6841 gaagtcacat cctttcattt tctttcttta atttattagc aagatagaca atcagcagta
     6901 gtaataccag acttgtcata atggaacctt ctatgccaaa agaaccacct gatagccaat
     6961 cttgattgcc ttgtggtaaa aaggtcatca gagacgttcc tgacggttga ccactaacta
     7021 aaatcccaaa gagattaccc tgagcaaaat tccaagcacc atgaatacct gcaacacccc
     7081 aaactgtatc agttttgaga aggtaaagag ccatggcaac tccgaataaa aagagattta
     7141 ctagagatag aggggtgagg ccagaattgc ccatatgaag cagggtaaag aacaggctag
     7201 atataagaat agctagtttt agattggttc ttgaggccaa ttgaggaagt agccaagcac
     7261 gggccaccac ttcttctgct gtcccctgta aaatccaaaa tgggatagta aagacgacaa
     7321 aggcaagaga ataaggattc aagtgaatgg attccaaacg atattgacct aagaccacta
     7381 aacctaacaa ggtcagaaga aaaagtgcca ggcctagact aaatcctttc agaagattgc
     7441 tgaggaaatt ctctctataa aatcccaagg ttcgaatagg tcttttctca acttttcttg
     7501 tccatctgaa cacagtgtta agaataaaac caaaggccag taatcctaaa attaaacgaa
     7561 agtcacttgt tttatccgtc aagctctgat gcaaggtctg caagtagctt gcaagatttt
     7621 gaccagcctc tccaaaattt ctagcaagtc cgatgagagc taacaaacta ataccatata
     7681 aagtataagc cacaaactct cctatgaaga caaaaacaaa ggctaacaag gggcttgtgt
     7741 aaatgtttaa attcattttt gaagcttgga agtctttaaa tattcttttg ttcttcatat
     7801 ccccgtcct
//
LOCUS       DAVKJD010000077         7744 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKJD010000077 DAVKJD010000000
VERSION     DAVKJD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.077"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            106..1776
                     /locus_tag="VW157_001712"
     CDS             106..1776
                     /locus_tag="VW157_001712"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9552981.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2105..3280
                     /gene="mutY"
                     /locus_tag="VW157_001713"
     CDS             2105..3280
                     /gene="mutY"
                     /locus_tag="VW157_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9552982.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            3335..4039
                     /locus_tag="VW157_001714"
     CDS             3335..4039
                     /locus_tag="VW157_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9552983.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            4032..5381
                     /gene="vicK"
                     /locus_tag="VW157_001715"
     CDS             4032..5381
                     /gene="vicK"
                     /locus_tag="VW157_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9552984.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            5383..6192
                     /locus_tag="VW157_001716"
     CDS             5383..6192
                     /locus_tag="VW157_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9552985.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVEVDFKVYDTSPDTATPLTEI"
     gene            6283..6525
                     /locus_tag="VW157_001717"
     CDS             6283..6525
                     /locus_tag="VW157_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9552986.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            6527..6781
                     /locus_tag="VW157_001718"
     CDS             6527..6781
                     /locus_tag="VW157_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9552987.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQINVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            6828..7523
                     /locus_tag="VW157_001719"
     CDS             6828..7523
                     /locus_tag="VW157_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEU9552988.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLVKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIILVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLLGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSS"
BASE COUNT         2368 a         1332 c         1727 g         2317 t
ORIGIN      
        1 tcttaaaaac agagatattt acgaacgttt agagagtttt aggatgttaa aaatcttgtt
       61 ttgtgatata atgaattatc atataagagg ttagaggaat tttgaatgaa aacagatatt
      121 gaaatcgcac agagtattga gttgaagcca attgttgatg ttgtagagaa acttggtatt
      181 tcttacgacg atttggagtt gtacggaaag tacaaggcta aactcagctt tgataaaatt
      241 cgtgcagttg agagcaatcc agtcggtaaa ttgatcttgg ttactgccat caacccaaca
      301 cctgcaggtg aaggaaagtc gacgcttacc attggtcttg cggatgcctt gaacaagatt
      361 ggcaagaaaa ccatgattgc tatccgcgaa ccgtctcttg gtccagtcat ggggatcaag
      421 ggtggtgctg ctggtggtgg gtatgcacaa gttctgccaa tggaagacat caacctccac
      481 tttactggag atatgcatgc tattacaact gccaacaatg ccctttctgc cttgattgac
      541 aaccacttgc accaagggaa tgagctggga attgatcaac gtcgtatcct ctggaaacgc
      601 gttgtggact tgaacgaccg tgcgctccgc catgtgactg ttggtcttgg tggtcctcta
      661 aacggtattc cacgtgagga tggttttgat attacagttg cttcagaaat catggcaatt
      721 ctttgcttgg caacggacat cgaggacttg aaacgtcgtt tggcgaatat cgttattggt
      781 tatcgctatg accgtacgcc tgtttctgta ggtgatttgc aggttgaggg tgccttggct
      841 ttgattttga aggatgctat taagccaaac ttggttcaga caatttacgg tacacctgcc
      901 tttgtacacg gtggtccatt tgccaatatc gctcatggct gtaactctgt tttggcgacg
      961 acaacagccc ttcacttggc tgattacact gttactgaag ctggttttgg tgcggatctt
     1021 ggtgctgaga aattccttga tatcaagaca ccaaacttgc caacatctcc agatgcagtt
     1081 gttattgtcg caacccttcg tgcccttaag atgaatggtg gtgtggctaa agacgctctg
     1141 actgaagaaa atgtagaggc agttcgtgca ggttttgcta acttgaaacg ccacgttgaa
     1201 aatatccgta agttcggtat tccagcagtt gtagctatta acgaatttgt atctgataca
     1261 gaagctgaaa ttgcagtctt aaaagaactc tgtgcctcaa tcgatgtacc agttgaattg
     1321 gctagtgtct gggctgatgg agcagaaggt ggagtagcac ttgccgaaac agttgttaag
     1381 acaattgctg aaaatccagc taactataaa cgtttgtacg ataatgacct ttctgtccaa
     1441 gaaaagattg aaaaaattgt cactgaaatc tatcgtggta gcaaagtgaa ctttgagaag
     1501 aaagctcaaa cacaaattgc tcaaatcgtt caaaacggtt gggacaaatt gccaatttgt
     1561 atggctaaaa ctcaatacag tttctcagac aatccaaatg cgcttggagc accagaaaac
     1621 tttgaaatta ccattcgtga attggtacca aaattaggtg caggcttcat cgttgcctta
     1681 actggtgatg ttatgaccat gccaggtctt ccaaaacgtc cagcagctct caacatggat
     1741 gttgaaagcg atggaactgt gctaggcttg ttctagtgga ttgaaattga ctttaagtga
     1801 gtttggaaat aatatccaag ctcatttttt ctcagactgc aagcagttca tcaataagat
     1861 ttatactctt cgaaaatctt ttcaaactac gtcagcttca ccttgccgta ggtaatatga
     1921 tactgactct gtcagttcta tccacaacct caaaacagtg ttttgagcaa cctgcggcta
     1981 gcttcctagt ttgctctttg attttcattg agtataagta gatagaaaag ttatatacta
     2041 tctattgaaa atattataaa tcagatacaa agacaggtga aaccttctgt gttatactag
     2101 agatatgtta gatttgaaag aatacggtat cgtcatgtgg ccggaggaga aagtcatttc
     2161 tttccgtgag aaacttctcg cttggtatga tgaaaacaaa agagatttgc cttggaggag
     2221 aagtaaaaat ccttatcaca tctgggtatc tgaaatcatg cttcagcaga ccagggtgga
     2281 tacagttatc ccttactacg aaagattttt ggactggttt ccaactgtcg aaagtctggc
     2341 aactgcgcct gaggagagtt tactgaaagc ttgggagggc ttgggttatt attctcgagt
     2401 tcgcaatatg caggctgcag cccagcagat tatgactgac tttggtggcc aatttccaaa
     2461 tacctatgaa ggaatttcca gcttgaaagg gattggacct tacacagcag gagccatttc
     2521 cagtattgct tttaacttgc ctgagccagc tgtagatggt aatgtcatgc gggtcttggc
     2581 gcgtctgttt gaagtcaacc acgatattgg gattccaagt aatcgaaaaa tttttcaggc
     2641 aatgatggag atcttgatta atccagatcg tccgggtgat tttaatcaag ccttgatgga
     2701 cttagggtct gatattgagt ctcctgtaaa tcccagacct gaagaaagcc cagtcaagga
     2761 ctttagtgcg gcatatcaga atggaaccat ggaccgttat ccaattaaat ctcctaagaa
     2821 aaagccggtt cctatttatc ttaaagcctt agttgtgaaa aattctcaag gacaattttt
     2881 acttgaaaaa aatgaaagcg aaaagctctt ggcaggtttt tggcatttcc cctttataga
     2941 agttgataac ttttcgcaag aagagcagtt tgacctcttt catcaggttg cggaagaaag
     3001 tgtgaactct ggccccagtc cagaagagag tttccagcag gactatgacc tagatgttga
     3061 ttggcttgat gtttgttttg atacagtcca gcatgttttt agtcatcgaa aatggcatgt
     3121 acagattgta gcaggtcagg tgagtgactt ccatgatttt tcagataggg aagttcgttg
     3181 gctttcacca gaagaattca agaattaccc acttgccaaa ccccaacaaa aaatctggca
     3241 ggcttatgca caagccaact tagacagtag ccaagactag ctattgtgtc ttctgactat
     3301 tttttggtat aatagtagag aaaaaggtga acatatgaaa aaaatactaa ttgtagatga
     3361 tgagaaacca atctcggata ttatcaagtt taatatgacc aaggaaggtt acgaagttgt
     3421 aactgctttt aatggtcgtg aagcgctaga gcaatttgaa gcagagcaac cagatattat
     3481 tattctggat ttgatgcttc cagaaattga tggtttagaa gttgctaaga ccattcgtaa
     3541 gacaagcagt gtgcccattc ttatgctttc agccaaagat agtgaatttg ataaggttat
     3601 cggtttggaa cttggggcag atgactatgt aacaaaaccc ttctccaatc gtgagttgca
     3661 ggcgcgtgtt aaagctcttc tgcgtcgttc tcaacctatg ccagtagatg gtcaggaagc
     3721 agatagtaaa cctcaaccta tccaaattgg ggatttagaa attgttccag acgcctacgt
     3781 ggctaaaaaa tatggcgaag aactagactt aacccatcgt gaatttgagc ttttgtatca
     3841 tttagcatcg catacaggtc aagtcatcac gcgcgaacac ttgcttgaga ctgtctgggg
     3901 ttatgactat tttggtgatg tccgtacagt tgatgtgact gtacgacgtc tgcgtgagaa
     3961 gattgaagat acgcccagcc gaccagagta tatcttgacg cgccgtggtg tagggtatta
     4021 catgagaaat aatgcttgat ttactgaaac aaaccatttt taccagagat tttatcttta
     4081 tcctgatttt gttaggtttc atccttgttg tgaccctctt attactggaa aatagacgtg
     4141 ataatattca gttgaagcaa atcaatcaaa aggttaaaga tttgattgca ggagattatt
     4201 ccaaggttct tgatatgcaa ggtgggtctg aaatcaccaa tattaccaat aatttgaatg
     4261 acttgtcgga ggttattcgt ctcactcagg aaaatctaga acaagagagt aagaggctaa
     4321 atagtattct gttttatatg acagatgggg ttcttgcgac caaccgtcgg ggtcagatta
     4381 tcatgattaa cgatacagcc aagaagcaac tggggttggt taaggaagat gttctgaata
     4441 gaagcatttt ggaattgctc aagatagaag aaaactatga attgcgtgat ttgattaccc
     4501 aaagtccaga attgttgcta gattcccaag atatcaatgg cgaatatttg aaccttcgag
     4561 ttcgctttgc cttgatacgt cgagagtctg gctttatttc aggtttggtg gctgttttgc
     4621 atgatacgac ggagcaggag aaggaagaac gcgaacgaag actctttgtt tccaatgtta
     4681 gccatgagtt acggactcct ctgactagcg taaaatccta tcttgaagcc ttggatgagg
     4741 gggctttgtg tgaaactgta gcaccagact ttatcaaggt ttctcttgat gagaccaacc
     4801 gtatgatgcg catggtgacg gatctcctcc atctttcacg tattgataat gctaccagtc
     4861 acctagatgt ggaactgatt aacttcactg cttttattac ctttatcctc aatcgttttg
     4921 acaagatgaa aggacaggaa aaggagaaaa aatatgagtt ggtgagagat tatcccatca
     4981 attctatctg gatggaaatt gatacagata agatgacgca ggttgtcgac aatattttaa
     5041 ataatgctat taagtattcg ccagatgggg gtaaaatcac tgtcagaatg aagacaactg
     5101 aagaccagat gattttatcc atttctgacc acggtttggg gattcctaag caggatttac
     5161 cacgtatctt tgaccgtttc tatcgtgtgg atcgtgctag aagtcgtgca caaggtggta
     5221 caggtctagg actgtctatc gctaaagaaa ttatcaaaca acataagggc tttatttggg
     5281 ccaagagtga atacggcaag ggttcaacct ttaccattgt gctcccttat gataaggatg
     5341 cagtgaaaga agaagtatgg gaggatgaag tagaagacta gaatgagtga aataggcttt
     5401 aaatacagta ttttagcgtc gggttccagt ggaaattctt tttatctgga aacctcaaaa
     5461 aagaagcttt tagtagatgc aggcttgtct ggcaagaaaa ttaccagtct gctagctgaa
     5521 attaaccgta agccagaaga cctggatgcc atcttgatta cccatgagca ttcagatcat
     5581 atccatggag taggcgtttt ggctcgcaag tatggtatgg atctttatgc caatgaaaag
     5641 acctggcaag ctatggaaaa tagtaaatat cttggcaagg tggattcttc gcaaaagcat
     5701 atttttgaaa tgggtaaaac caaaaccttt ggagatatcg acatcgagag ttttggtgta
     5761 agccatgatg cagtcgcacc gcagttctat cgctttatga aggatgataa gagttttgtc
     5821 cttttgacag atacaggtta tgtcagtgac cgtatggcgg gcattgtcga aaatgcggat
     5881 ggctatctta tcgaggccaa ccatgatgta gagattttgc gatcaggttc ttacgcttgg
     5941 cgactcaaac aacgaatcct atctgacctt ggtcaccttt ctaacgagga cggtgctgaa
     6001 gctatgattc ggacgctagg aaatcgtact aagaagattt accttgggca tttatctaag
     6061 gaaaacaata tcaaggaact ggctcatatg accatggtca atcagctggc tcaagctgat
     6121 ctgggagtcg aagtagactt taaggtttat gatacctcac cagataccgc aacaccattg
     6181 acagagatat agaaagaacg ctgagaaggt gttcttttta tattgactga acacctaaaa
     6241 agtaatacaa tggtgttacc attaaaaaag ggagcacaaa agatgactac tattacatta
     6301 aaagtttctg aagctgataa aacatttatg aaagcaatgg ctaagtttga aggagtttcc
     6361 ctttcggaac ttattcgcac caaaactctt gaagccctag aagatgaata cgatgctcgt
     6421 gtggcagatt tagcctatca agagtattta gaagacttgg aaaaaggagt tgaacccatt
     6481 acttgggaag aaatgatgca tgatttaggc ttgaaggatg aataatttgt ataaattagt
     6541 tccaacaaga cgtttcatca agcaattgaa aaaattggac cgttatacgc agaagctaat
     6601 tacaaactat ttacaaatca atgttttgga agacccaaga cgacacggaa aggctttggt
     6661 tggtaatcgc gttggtcaat ggcgctatag aattggtaat tatcgagtta tcgtacaaat
     6721 tgtagatgat gaattagttg ttgctactct agaagttggt catcggagag atatttattg
     6781 aattaaagct gagtaataac aatatagaaa gtaaggagag taaaaatttg aagaaaaaat
     6841 ggttttttgc agattattat gatacaacaa ttattttact tgctttaatc tctgttattc
     6901 tggtattact tggatttgct gagatgattg atttggacaa tccgccttat agtattattg
     6961 atttagtaat ctggggtgtt tttgtaattg attatagttg gcgctttttt attactaaaa
     7021 gaaaatggcg ctttattctt gaaaatattt ttgatttatt ggctattctc cctttaaatg
     7081 ctattttcac agtttttcga ttagggcgta tctttcgttt agttaaacta acaaaattac
     7141 tgaaactgac tcgtttactt agaataattg gacttacagg taaattagaa agaaaaatta
     7201 gtagattctt acggacaaat ggcctgattt atatcttata tgttaatatc tttattattc
     7261 tagtaggaag ttcaatttta tctgtagttg aagaaaaatc cttctcagat agtctttggt
     7321 gggctcttgt gacagtaact actgttggtt atggtgatat tgttccagtt tcccttttgg
     7381 ggaaatggct agcagtttta ctgatgcttg tcggtattgg aacaataggg atgctaacaa
     7441 gtgctttgac gaactttttt gtaaaggata atccagatga acagataaaa cttgataaac
     7501 tcaaagacga attatcgagc taaagaatat taatagagaa acaatctgaa aaaatcgaag
     7561 aactacattg aatgataaaa gacttagttg aaaaaatatc ataaaggtga gacaaaataa
     7621 aacttgattc gaggagaaat caagtaagtc ttgacacaat agaacgcata ttatcaggta
     7681 taaaaacttg atactatgcg ttttatagag aaataaaata aaatctgaac aaatcaatta
     7741 ggaa
//
LOCUS       DAVKJD010000078         7325 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKJD010000078 DAVKJD010000000
VERSION     DAVKJD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.078"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            511..733
                     /locus_tag="VW157_001720"
                     /pseudo
     CDS             511..733
                     /locus_tag="VW157_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(1449..2954)
                     /gene="pyk"
                     /locus_tag="VW157_001721"
     CDS             complement(1449..2954)
                     /gene="pyk"
                     /locus_tag="VW157_001721"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9552989.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(3013..4020)
                     /gene="pfkA"
                     /locus_tag="VW157_001722"
     CDS             complement(3013..4020)
                     /gene="pfkA"
                     /locus_tag="VW157_001722"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9552990.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            complement(4103..7204)
                     /locus_tag="VW157_001723"
     CDS             complement(4103..7204)
                     /locus_tag="VW157_001723"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9552991.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLEQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFDIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
BASE COUNT         2231 a         1612 c         1276 g         2206 t
ORIGIN      
        1 attaatttct agaaatgttt tagcagctac agcgtactat tccaaactca atcaactata
       61 aaacaatata gactagtaat ggttggactc aataagaatt gggtactaag ataggtataa
      121 gtaaaaacac cattagaaat tatgaaaaaa gggttaggtc tactaagaaa aatactatat
      181 ttgacttagt taaagtattc agtagcttga tcgatgccct ctttcctcca gttcaaaagg
      241 attctcctag cgatatccaa tctatctacg accaacggga accacccaga caaggtaaag
      301 ttctgaccta tgccgagagg caactgtatg atcagaaaaa cgaagtatcg taaaaatcat
      361 cagactggac gactacagac agactactta ctctcgtgtt aggtagttta tctctgcagg
      421 tagccgctcg atgcaggaca acgatttaga tatggagttt ccattatccc agacgactac
      481 gacccaatcg cttatgtcgt cagcaagtcc atggagccaa agataagaaa cagcgactac
      541 ctatttatca agaatacccc acagattgac tataatacca tcggcatctt ccaagtagac
      601 ggcactaact atgtcaagaa actccgccaa ggctacctca aaaaaactca atcccaaata
      661 ccccgacatc cacctagacg aaagcaacca tattcgtacc atcgggaaag ttgtcaggct
      721 atatagggag aatggcgata taaaacaaac agattttaaa agattcgaaa aactttgaaa
      781 aagtttattc tacatttcaa aaaaagaata agtgacctaa aatagattag attttgttaa
      841 acaatagtga tatgtaaatt tagagaaagg catttaagat cactttgcaa aaggcaaata
      901 ctaaactaga gaaaccgata aaccagcccc ttagttccca tattttcagg cacacactcc
      961 ttagtacact agctgagaag aatgctataa tggttagagt agaacacaaa aacgctaaaa
     1021 cagtaaataa catctagact cacgtaggta aaagaatgga gcaagctact ttggaggttt
     1081 taaataccat atcattaaat cgaaaatata tcagatcgaa tctagacaag tagattgcga
     1141 ttgctaaaac atttgtagaa attcatttga ctttctaatc aatttatgca tatcttattt
     1201 catactcaat gaaaatcaaa gagcaaacta ggaagctagc cgcaggttgc tcaaagcact
     1261 gctttgaggt tgtagataga actgacgaag tcagtaacat atatacggta aggcgacgct
     1321 gacgtggttt gaagagattt tcgaagagta tcaatccatt atgaaagttt tgcctatttc
     1381 ttaccctcta tacaaaaagc ctatcacaca agctctaaag ctggatatga taggttttta
     1441 tatttttctt aacgtactgt gcggatacgc attgtgtttg tgcgaacagc ttctcctact
     1501 ggcacaccag caacgataac gatatcatcg cctgactcaa cgagacctgc ttctaccgct
     1561 ttacgttcag cgatttcgaa catatcgtca gttgaagatg gagcatctgt caacattggg
     1621 ataacacccc agttcaacat caagccacgt tctgtcaatt cgtcaaatgt caatgctaag
     1681 atgtcagcat ttggacggta tttagaaatc aaacgtgcag tatgacctgt cttagtaaga
     1741 gttacaacca atttgatatc cattgagcta gtagcatctt taacagcaga agccattact
     1801 tctgtcttag agttacgctc aaatgaatct gaatcaagac gtccgtattc attaagaaga
     1861 gcttgagcgt tcttgtcgat tgtagccatt gtagttactg actcgagtgg gtatttaccg
     1921 tttgcagact cgcctgacag cattgtagcg tcagttccgt cgataacagc gttgaatacg
     1981 tctgatactt ctgaacgagt tgcacgtggt ttttcagtca ttgtttcaag catgtttgtt
     2041 gcagtgataa caactttacc tgcagcattg actttcttga taatcatttt ttgataaact
     2101 ggaaccattt cgaacggtac ttcgataccc atatcaccac gagcaatcat aataccatca
     2161 gctgcttcga tgatttcgtc taagttatcg ataccttgtt ggttttcgat tttagcgaac
     2221 aattgaacat gtccgtttcc agtttcttca cagattgcac gaacttcgtt cacatctttt
     2281 gcagtacgta cgaatgaaat tgcgatgaag ttgatacctt gttcaagacc gaaacggata
     2341 tcgtcgttat cgcgttcagc aagagctggg aaaggaattt tagtgttagg gatgttcaca
     2401 cctttttgtt tagcgatgat accatcgttt tcaacttcaa cttcaaattc acgagttgca
     2461 tcatctttag caaccacacg aagaccaagt ttaccatcgt caaccaaaac ttgacgacca
     2521 acttcaacat catcatagat atcaagagca ccagcaacgt tcaacgcaat cacttcacga
     2581 gttgatttga ttccttgttt agttgcaaca cgaatttttt caccagtttt gtatgaatat
     2641 tctttagctt caccttcgaa caattctgta cggatttcag gtccttttgt atcaagaagg
     2701 aaaccaactt ttttacctgc aattttttcc gcaagtttaa cagttgccat acgctcacct
     2761 tgttcttggt ggtcgccgtg tgagaagttg aatcggaatg tgttagcacc agcttcaatc
     2821 aatttagcaa tgtttttagc tgaagcttca acatcaagtt tttcacccca gtatccgtcc
     2881 tcaccgaatt ttttaccacc acggatttct accgcaggac ccaaagttgc aacgatcttt
     2941 acacgtttgt tcatgatttt tgtgactcct ttatatatat gacctttttg gtcttattaa
     3001 ctaaattgta aattatgaca agctcttatt caagctagat agctcaatat cggctttgtg
     3061 cgggttgtta accacaatct taccttctgc agtaaggcta aacaatgccc cttcttctgc
     3121 agtaccaaga attggatttt caaccatttt ttcgttacga ataccaaccg caacaccacc
     3181 gataccttct ttaagaagtt taacagcatg tgcacccata cgtgacgcca aaacacggtc
     3241 acgcgcagtt ggagaaccac cacgttgaat atgtccaagt tctgttacac gaaggtcgct
     3301 tgtatctcca gcttctttaa gtttttgacc aaattcagcc gctgacatca caccttcagc
     3361 taagacgatg atattgtgtt ttttaccaca ttcataacca gctttgatgc ttgctacgat
     3421 atcttccatc ttgaagcctg cttcagggat gatgatttca tcagcaccag ttgcaatacc
     3481 agcccaaaga gcgatatcac cagcgttacg tcccataact tcgattacaa aagtacgacg
     3541 gtgacttgat gatgtatcac gaatcttatc gatagcgtcc atggcagtag taaccgctgt
     3601 gtcaaaaccg attgtaaagt cagtaccaac gatatcgtta tcgattgtac ctggaagacc
     3661 aatagctggg aagccatgtt cagtcaaacg catagcgccg tggtaagatc cgtcaccacc
     3721 gataacaact acaccttcaa ttccgtgttt tttcaattgc tcaatccctt taagttgccc
     3781 ttcaagttga gcgaactctg gataacgagc tgagtgaaga aaagtaccac cacgagaaat
     3841 gatgtcccct actgaagctg catctagggg atgaatttca ccggcaacca taccagcata
     3901 tccgtcatag ataccaaaca cttccattcc ttctgaaatt gcttgacgaa caactgcacg
     3961 gatggcagcg ttcataccag gtgcgtctcc gccactagtc aaaacagcaa tacgtttcat
     4021 attggtttat gctccttttt cttttaacat tcttcttgat tatatcacat ttgattttaa
     4081 aattcttcta ttttccgtat ttttagcgat aaatcgtttt cataacgatt tcattcaatt
     4141 tctcctctaa ttcattggat ttagctacaa aatgatgggg agaaacgatg gttttctgtt
     4201 cctcttcata ccggatgatg actgggattg ggcctttaaa ttgttctaaa atgcgtgaaa
     4261 tttcttgatc cgattcatga tttttcacct gtatccaaaa gcgttcagca actgcttctc
     4321 ttatttcttg tgcaatcatt tgcagacggc catcacgtga ttgtattttt ccttttacat
     4381 agtagaaggc tccctctttt atttcctgtc caacctgacg atataagtct gaaaagagag
     4441 tgacatccaa ttttttctta ctatcatctg cctgtaagaa ggccatattt tcaccctttt
     4501 tggtacgaat cacttttatt ttctgaactt caaccaagat aatagcatag ctattttctg
     4561 acaaatttcc gattggggta atcgggtaaa tagccttact tgcaatagct tgtagtggat
     4621 gtttgctgac acctatccct aaaagctctt gttccatata aaatttttct tgttccgtcc
     4681 aatcttccga ttcctgccaa ctataaatag catctccaaa caaacttccc aactctttca
     4741 caaattcaaa tagattagct aaattattaa atactttttg acgatttttt tcaaatgaat
     4801 cgaaaagacc aacttttacc aaaggttcta gcagaggaag tttcagataa ttctcaggta
     4861 atttagctat aaaatcttca atgttagaat aaggtctatt ttcaataatc cagagagcta
     4921 aatcattact gactccttta atggatttca aacctagata gatggccttg ttggcaattt
     4981 tatcgtgata gggaatggtg ttgatggata gagaggctac ttcaaaacct gcttcaagtg
     5041 catctattaa gtaatcactg ttggaagaat ttaacatgac ctgataaaaa atggctggat
     5101 aatgtgtttt aaaataagcc aactggaagg ccaaggctga gtaggcatag gcgtgtgacc
     5161 tgttaaaacc ataacctgca aacttctcca taacatcaaa gacctgctct gctttttcca
     5221 cagtatgacc agcttctaat gaaccttgaa taaaggaagc cctcatctca tgcatggcag
     5281 aggcatcctt tttccccata gcccgacgca aaatatcggc tttcccaaga ctaaatccgg
     5341 caaatcgctg ggcaacctgc ataacctgct cctgatagag cattatgccg taggttggag
     5401 ccaaaatatc ctccagtact ggatccagaa cagtcacttc ttcctgccca tgctttcttg
     5461 ccacaaaatt attgatatag tcactagcac ccggtcgatt tagagaagta gtcgcgacga
     5521 catcttcaaa acagactggt tgcacacgct taagcagacg aatggcacct ggttgctcaa
     5581 attgaaagat accttttgta tttccagagg caaataaagc taacgtttct ttgtcttcca
     5641 aatctatttc ttcaattttc agatgaatac cttctgtttc agcaagcaac tcttgcatct
     5701 tctggacaaa ggtcaaattt cgtagtccca gaaagtccat cttcaaaagt ccgctagcct
     5761 caactccatg agcatcatac tgagtcagtg gaatttcatc accatacttt agaggaatgt
     5821 agttggttaa atcttggtca ctaattacaa caccagccgc atggacagag gtttgccttg
     5881 gatagccctc tatcttgcaa gcaatatcaa aagctttttg gtattctaac ttactattga
     5941 tttgctgacg aaactggaga ttgccctcat aggccgactt aagattgtca cgaaaactga
     6001 ttttcttagt aattgcagat aattcatact ctggcacacc aaagcgtttc aagacatctc
     6061 gaagagcttg cttggctcca aaggttgaaa aagtaacgat ttgtgccgca tgtttactac
     6121 catatttatt accaacatat ctgataaaat ctggacgata aatatctggg atatcaatat
     6181 caatatcagg catggtatag cgttcacgat taagaaagcg ttcaaaaatc agatttttct
     6241 ctactgggtc aatccccgtg atgtctaagg cataagaaac caaactgcct actgcagaac
     6301 cccttcccat tcccatataa tagccattcg atcgtccaaa acgcaacaaa tcccaaacaa
     6361 ccaagaaata atcatcaaag cccatatcat gaataacaga caattcttgt tctagtctat
     6421 cttgatattc tttactagtc aaccccttct gaacaagccc cagttcagca cgctctctca
     6481 actcctctac tgctggtcta gctggattaa aacgaggcag tttcagacta gtatccaagt
     6541 cgtaagaaat gcctgaaata agcttttcta aattgtccaa agcttgcgga aaacgctctt
     6601 ggaatagttt ctctaaagaa cttgctgata taaagacatc ttgtctcgaa cgcaagggaa
     6661 cttctctgag cggtagattt tctttaatcg ctgttaaaac ttgaagaact tctctatccc
     6721 tgctttcaaa agcgttgacc cgataaagag gtaagatagg atgatgaaat tcgcttgcta
     6781 gtgtttctgg ataaacccct atatagtaat cacagcctag ttctaacgac tcaactctat
     6841 caaaataagg cacaatgacc gcgatatcct ccaggtactg ggacaggact gaccaagttt
     6901 tctccccctg catcttggct gtcgaaagct tcatcaactg ctgatagccc acactagata
     6961 gagctaaaaa gcgcaaattc acttcctgat catctacaaa cactgtcatt tcaagcccta
     7021 gcaaaggatg aatgccgtat ttttttgtaa tctctagaaa gtcgaaagcg ccataaagat
     7081 tgtcaatatc catcatagcc aaatgagtgt agccgtattc tttagctgct ctcacatact
     7141 tttcgatcga aatgacgctt tccataaaac tatagactgt ttttgtatct agttgtgcga
     7201 tcaatttaca cttctcctct atccttctca ctatattata ccattttcac ctataaatgg
     7261 cttctcttga gaaaaatttc atcagcttac taatttattt tctattcatt tgtattttcg
     7321 tcatc
//
LOCUS       DAVKJD010000079         7206 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKJD010000079 DAVKJD010000000
VERSION     DAVKJD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.079"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..121
                     /locus_tag="VW157_001724"
     CDS             <1..121
                     /locus_tag="VW157_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEU9552992.1"
                     /translation="SIYLTHALFGVCQTWIAHGKKESSQEITDFLMKMLGDTN"
     gene            complement(162..235)
                     /locus_tag="VW157_001725"
     tRNA            complement(162..235)
                     /locus_tag="VW157_001725"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(199..201),aa:Asn,seq:gtt)
     gene            complement(241..312)
                     /locus_tag="VW157_001726"
     tRNA            complement(241..312)
                     /locus_tag="VW157_001726"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(277..279),aa:Glu,seq:ttc)
     gene            complement(364..1116)
                     /locus_tag="VW157_001727"
     CDS             complement(364..1116)
                     /locus_tag="VW157_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9552993.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1113..2438)
                     /locus_tag="VW157_001728"
     CDS             complement(1113..2438)
                     /locus_tag="VW157_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9552994.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(2459..2584)
                     /gene="comC"
                     /locus_tag="VW157_001729"
     CDS             complement(2459..2584)
                     /gene="comC"
                     /locus_tag="VW157_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9552995.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            complement(2749..2822)
                     /locus_tag="VW157_001730"
     tRNA            complement(2749..2822)
                     /locus_tag="VW157_001730"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2786..2788),aa:Arg,seq:tct)
     gene            complement(2866..3345)
                     /gene="rlmH"
                     /locus_tag="VW157_001731"
     CDS             complement(2866..3345)
                     /gene="rlmH"
                     /locus_tag="VW157_001731"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9552996.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            3528..4709
                     /locus_tag="VW157_001732"
     CDS             3528..4709
                     /locus_tag="VW157_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9552997.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            4767..5525
                     /locus_tag="VW157_001733"
     CDS             4767..5525
                     /locus_tag="VW157_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9552998.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            5738..7099
                     /gene="dnaA"
                     /locus_tag="VW157_001734"
     CDS             5738..7099
                     /gene="dnaA"
                     /locus_tag="VW157_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9552999.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         2484 a         1307 c         1144 g         2271 t
ORIGIN      
        1 tagcatctat ctaactcatg cactttttgg tgtctgccaa acttggattg cacatggaaa
       61 aaaagaaagt tctcaagaaa taacagactt ccttatgaaa atgcttggtg atacaaattg
      121 atacaaaaag aggaacacag ctgtgttccc ttttatctat actccgccag taggactcga
      181 acctacgaca tcatgattaa cagtcatgcg ctactaccaa ctgagctatg gcggataaaa
      241 tagtccgtac gggattcgaa cccgtgttac cgccgtgaaa aggcggtgtc ttaacccctt
      301 gaccaacgga ccttctatct gtagcagata taaccattat atcaatttct tgctaattgt
      361 caatcacttt tgagattttt tctctaaaat atcttttaat tttctaattt ttaatcttga
      421 aataggacaa cgatggtctt catagaaaac aatttctaag ttttttcgat caatttctct
      481 gatattgcct atatttacca aaaatgactt gtgaggagaa taaaatcgct gagtatgttt
      541 gtccttttcc tgaatatctg tcatggtacc ataaaactct tttgcaaaat tcttaccaat
      601 aatgcgcaat ttatgagaga cccctgttgt ttcaatatac aaaatatcat ggtaaggaat
      661 ttttaaatca tttcctttgt aattgtagtc gaaataatct acaacatctt cattttcaag
      721 taacatactc ttcgtgtaga agatattttg ctcaattctc ttcttaaaca tctcatcatt
      781 gatatcctta tcaacaaaat ctagggctga tacctggtat ttataggtta gagtcgcaaa
      841 ctctgatcga ctagtgataa agacgataat agcgtaagga ttgtaatgac gaatgagctg
      901 agccacttca aatccctttt tctcaattcc atgaatatcg atatctagga aataaagttg
      961 atttacttca tcattttcaa tgtattcttc aaattcacgg acttttcccg ttgtcttgta
     1021 tgatattgga atattcgatt ctttcgaaat ttcatccaat attctctcta gtctcacttg
     1081 atgttcaata acatcttcta aaattaaaac tttcattcaa attccctctt aaatctaatg
     1141 atttgtctaa atgtactgcc ttccatctct gtttctaaaa taatattgtt gtacttatct
     1201 agtagttctt tcacattatt taatccgact ccgcgatttc ttcccttagt ggagaatcct
     1261 aaggcaaata gatctcctga aggagtcatc gtcattttac atgaattctg aatcacaata
     1321 actgtttcag tttccatctt aataactgct acttccatct gctttttata gctatcagcc
     1381 gatccttcga cagcattatt caataaaacg ctcatgatac gaaccaaatc caatagttca
     1441 attggaagct tggtaatcgt atcttttact tccagtgtaa actctacacc attatttcga
     1501 gcatagacaa ttgactgagc aaccaaactt cgtaaagctg agtcttctat gttgttcaaa
     1561 tcaaagtaag tgtacttatc tgaacgcaat ttatgatttg ctttgactaa aacttcattg
     1621 taaattctgt caatttcctg taaattacca ctgtcaattg ccatctgcat gctgacaagc
     1681 attccagcat aatcatgtcg aaaaccacgg atttcattat acagaccaac aatttcatct
     1741 gtgtaattct gtaaatgttt ctgttcaaat ttcttctgct tcaaagcaat ctctttctcc
     1801 atttgaactt tatgagaatt cattgcaaag aaggtcaaaa ggagagagat aaagacaata
     1861 gatgacaaaa tacttccaaa actattcaaa tgtttaatcg tacttaccat atctgaaacg
     1921 aaagatacaa tatgtagcaa tagtaaagca aaaaatactt ttttcaagaa aggataaagg
     1981 tagtcctcgt caaaataggc tagttccaaa tggaaatagt aaatgatttt taatgtaaca
     2041 aaataggtta acaccgtcac aacgaaaaag aatgggaaat gatattgtaa aacaaaatta
     2101 tctcctgtta tagaggagaa agttacggac agaaagttat gagtgctctc atataaaaga
     2161 gatagtagta aacttaggaa tagtcctcta tccctctcat actgtttcat ccatcgaaaa
     2221 taggaatata atcccaaagg aaataaaaat ctttcaatcc ctaacccatc taaatataga
     2281 atatataagg gaaaatcaaa tactatttct gttagtaatg tataagcacc aaaaacgaat
     2341 aattcttttc tatttatttg acctttacaa ataaaatggt aactgtgact aataattaaa
     2401 aaatgaacaa taaccgtccc aaatccaaat aaatccatta ctctttcccc ttatttcatt
     2461 acttttttct ttgtaaaata aaatcacgga agaattttga caacctcatc tccccacctt
     2521 taatcttttg taagtctttt tccttcaaag ctacaaactg ttccaattta actgtgtttt
     2581 tcataataaa atctcctaaa atgttttttc ttgtaagcta acttacaaaa accattatac
     2641 aaaatggaat ttcgttttag ataaaattct ctcaactgtc atttttttct cccaaagtgt
     2701 acttttttaa gaaaaaagcc gggaaaattc ccagctttgc tattatattg atcccagcag
     2761 gattcgaacc tgcgaccgtt cgcttagaag gcgaatgctc tatccagctg agctatgaga
     2821 cctaatacaa ttattctacc aaaaattcaa ttaaaagtca attttctatt tatggtaggg
     2881 gaatccctgc tgaatcgtaa aagcgcgata gatttgttca acaagaacta gtctcattaa
     2941 ctgatggggt aaggttaggc gaccaaaact gacagaaaga ttggctctat tttttacaga
     3001 tgatgacaat cctaaacttc ccccaataat aaaagtaaga gtagaaaatc cttttataga
     3061 agtttcttct aactgcttac taaattcttc tgagaagaaa gttttccctt caatggctaa
     3121 cacaataacg aaatcacggt cagcaatttt tgataaaatt ctctgacctt ctatttctaa
     3181 aatcttttga ttttctgatt cactggcctt atctggtgtt ttttcatctg ataactcaat
     3241 catttcaaac ttagcaaatc tagaaattcg ttttgaatac tctgcgatac catcttttaa
     3301 atacttttct ttcagtttcc caactgttac aactttaatt ttcatgactc tattctaaca
     3361 tattctctat tttttcacat cttattcaca aaataaaaaa tagatttcaa ttaagaaaat
     3421 cacagtttca aaagagttat ccacagtttg tgtaaaactt ttgtgtttaa gttataatta
     3481 agctagtcag tttatacttt cagtaattca aacatatgga ggcaaatatg aaacatctaa
     3541 aaacatttta caaaaaatgg tttcaattat tagtcgttat cgtcattagc ttttttagtg
     3601 gagccttggg tagtttttca ataactcaac taactcaaaa aagtagtgta aacaactcta
     3661 acaacaatag tactattaca caaactgcct ataagaacga aaattcaaca acacaggctg
     3721 ttaacaaagt aaaagatgct gttgtttctg ttattactta ttcggcaaac agacaaaata
     3781 gcgtatttgg caatgatgat actgacacag attctcagcg aatctctagt gaaggatctg
     3841 gagttattta taaaaagaat gataaagaag cttacatcgt caccaacaat cacgttataa
     3901 atggcgccag caaagtagat attcgattgt cagatgggac taaagtacct ggagaaattg
     3961 tcggagctga cactttctct gatattgctg tcgtcaaaat ctcttcagaa aaagtgacaa
     4021 cagtagctga gtttggtgat tctagtaagt taactgtagg agaaactgct attgccatcg
     4081 gtagcccgtt aggttctgaa tatgcaaata ctgtcactca aggtatcgta tccagtctca
     4141 atagaaatgt atccttaaga tcggaagatg gacaagctat ttctacaaaa gccatccaaa
     4201 ctgatactgc tattaaccca ggtaactctg gcggcccact gatcaatatt caagggcagg
     4261 ttatcggaat tacctcaagt aaaattgcta caaatggagg aacatctgta gaaggtcttg
     4321 gtttcgcaat tcctgcaaat gatgctatca atattattga acagttagaa aaaaacggaa
     4381 aagtgacgcg tccagctttg ggaatccaga tggttaattt atctaatgtg agtacaagcg
     4441 acatcagaag actcaatatt ccaagtaatg ttacatctgg tgtagttgtt cgttcggtac
     4501 aaagtaatat gcctgccaat ggtcaccttg aaaaatacga tgtaattaca aaagtagatg
     4561 acaaagagat tgcttcatca acagatttac aaagtgctct ttacaaccat tctatcggag
     4621 acaccattaa gataacctac tatcgtaacg ggaaagaaga aactacctct atcaaactta
     4681 acaagagttc aggtgattta gaatcttaat tgacatctat gtaaagaaag ctttacataa
     4741 gagaaaagat gtgttagtgt agaatcatgg aaaaatttga aatgatttct atcacagata
     4801 tacaaaaaaa tccctatcaa ccccgaaaag aatttgatag agaaaaacta gatgaactag
     4861 cacagtctat caaagaaaat ggggtcattc aaccgattat tgttcgtcaa tctcctgtta
     4921 ttggttatga aatccttgca ggagagagac gctatcgggc ttcactttta gctggtctac
     4981 ggtctatccc agctgttgtt aaacagattt cagaccaaga gatgatggtc cagtccatta
     5041 ttgaaaattt acagagagaa aatttaaacc caatagaaga agcacgcgcc tatgaatctc
     5101 tcgtagagaa aggattcacc catgctgaaa ttgcagataa gatgggcaag tctcgtccat
     5161 atatcagcaa ctccattcgt ttactttcct tgccagaaca gattctctca gaagtagaaa
     5221 atggcaaact atcacaagcc catgcgcgtt ccctagttgg gttaaataag gaacaacaag
     5281 actatttctt tcaacggatt atagaagaag atatttctgt aaggaagtta gaagctcttc
     5341 tgacagagaa aaaacaaaag aaacagcaaa aaactaatca tttcatacaa aatgaagaaa
     5401 aacagttaag aaaactactc ggattagatg tagaaattaa actatctaaa aaagacagtg
     5461 gaaaaatcat tatttctttt tcaaatcaag aagaatatag tagaattatc aacagcctga
     5521 aataaggctg ttcttttatt tttttatctc acaaggttat ccactatgtt tttcgataaa
     5581 aagcttaata aatcaataat ttcttctttt atccccaacc tgtggataaa gtttagtaac
     5641 attgtggatt atttttcaca gcttgtggaa aattcttgct atctatggta aaatatctct
     5701 agtattaaac ttttaaatag taaaggagga gaaaggattg aaagaaaaac aattttggaa
     5761 tcgtatatta gaatttgcac aagaaagact gactcgatcc atgtatgatt tctatgctat
     5821 tcaagctgaa ctcatcaagg tagaggaaaa tgttgccact atatttctac ctcgctctga
     5881 aatggaaatg gtctgggaaa aacaactaaa agatattatt gtagtagctg gttttgaaat
     5941 ttatgacgct gaaataactc cccactatat tttcaccaaa cctcaagata cgactagctc
     6001 acaagttgaa gaagctacaa atttaactct ttatgactat agtccaaagt tagtatctat
     6061 tccttattca gatacgggat taaaagaaaa gtataccttt gataacttta ttcaagggga
     6121 tggaaatgtt tgggctgtat cagccgcttt agctgtctct gaagatttgg ctctgaccta
     6181 taaccctctt tttatctatg gaggaccagg ccttggtaag actcacttat taaacgctat
     6241 tggaaatgaa attctaaaaa atattcctaa tgcgcgtgtt aaatatatcc ctgccgaaag
     6301 ctttattaat gactttcttg atcacctaag acttggggaa atggaaaagt ttaaaaagac
     6361 ctatcgtagt cttgatcttt tgttaatcga tgatatacag tcactcagcg gaaaaaaagt
     6421 cgcaactcag gaagaatttt tcaatacctt taacgccctt catgacaagc aaaaacagat
     6481 tgtcctaacg agtgatcgta gtccaaaaca tctagaaggg ctcgaggaga ggcttgtcac
     6541 gcgttttagt tggggattga cacaaactat cacaccccct gactttgaaa cacgtattgc
     6601 cattttacaa agtaagacgg aacatttagg ctacaatttc caaagtgata ctctagaata
     6661 cctagctggg caatttgatt caaatgttcg agatcttgag ggagccatca acgacatcac
     6721 tttaattgcc agagtaaaaa aaatcaagga tatcactatt gatattgctg cagaagctat
     6781 tagagcccgc aaacaagatg ttagccaaat gctcgtcatc ccaattgata aaatccaaac
     6841 tgaagttggt aacttttatg gtgttagtat caaagaaatg aagggaagta gacgccttca
     6901 aaatattgtt ttggcccgtc aagtagccat gtatttatct agagaactaa cagataatag
     6961 tcttccaaaa attgggaagg aatttggggg aaaagatcat accacagtca ttcatgccca
     7021 tgccaaaata aaatctttga ttgatcaaga cgataattta cgtttagaaa ttgaatcaat
     7081 caaaaagaaa atcaaataat ttgtggataa cttttagttt tttatctttt ttatccacat
     7141 tttttaaaca agctaaaaaa cttgatatga cttgtttaaa ggctgttttc cacagatttc
     7201 acagac
//
LOCUS       DAVKJD010000080         6966 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKJD010000080 DAVKJD010000000
VERSION     DAVKJD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.080"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            251..703
                     /locus_tag="VW157_001735"
     CDS             251..703
                     /locus_tag="VW157_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553000.1"
                     /translation="MIYVLSNFLYAISDSIIYALNGIVLLVIVSKLGIPGDLGLDFIV
                     AIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTMMMNTSNDLFI
                     KAIELLPFYSLPVFVASNTMSISQYLVLITTIILLYFFTLKKSKKYSF"
     gene            1089..1298
                     /locus_tag="VW157_001736"
     CDS             1089..1298
                     /locus_tag="VW157_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9553001.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            1288..1776
                     /locus_tag="VW157_001737"
     CDS             1288..1776
                     /locus_tag="VW157_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553002.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            1892..3352
                     /locus_tag="VW157_001738"
     CDS             1892..3352
                     /locus_tag="VW157_001738"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9553003.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            3349..4173
                     /gene="nadE"
                     /locus_tag="VW157_001739"
     CDS             3349..4173
                     /gene="nadE"
                     /locus_tag="VW157_001739"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9553004.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAIEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGFHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            4523..5074
                     /locus_tag="VW157_001740"
     CDS             4523..5074
                     /locus_tag="VW157_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9553005.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLIYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            5789..6481
                     /locus_tag="VW157_001741"
     CDS             5789..6481
                     /locus_tag="VW157_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9553006.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
     gene            6535..6924
                     /locus_tag="VW157_001742"
     CDS             6535..6924
                     /locus_tag="VW157_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9553007.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
BASE COUNT         2184 a         1183 c         1510 g         2089 t
ORIGIN      
        1 taaggaaaat ataagaaatt tatattcatc aggcgtaata tatctcagtt tgcttatctc
       61 gtttataccg ccgatattgc ttacattctt tattctaaag acttaaggga catcgcttgg
      121 tattaagcat atttcaaact tttatgctat gctcggtatg ttaatggctg ttatacatgc
      181 taatcgagtc attagtagag atttttccca caatacggta agtttgtttt ataatcaaca
      241 gaagaatcgg atgatttacg tcttgtctaa ttttctatat gccatctcag attccattat
      301 ttatgctttg aatggcattg tgttactagt catagtaagt aaattgggta ttccaggtga
      361 tttaggatta gattttatag tagctattgt agtcaataca attttgttag tcctgtttta
      421 ttttctatta tcttacattt tctatttata caaattgaaa agtggcttgg tatttggtat
      481 tttagtagct ttactactct ttatccctaa tatattaaat acgatgatga tgaatactag
      541 taatgatttg tttatcaaag caattgaact tcttcctttt tattctttac ctgtatttgt
      601 ggcttcaaat acgatgtcta ttagtcagta tcttgtgcta atcactacaa tcattttatt
      661 gtactttttc actctcaaga aaagcaagaa gtattcattt tagtttcatt tagtattatt
      721 ttgcatacct aaaatacagt aaaaaaatca gtcatcttgg tatgctcctg ctttcactat
      781 tcaacacgtt tttgacttat actaggctca tttccaaaag cattatataa tagtgatatg
      841 aaaccaacta aactaaacaa gaaatataag caataaaaac tcgtttaaaa gattctacta
      901 aagctaatac taaataaaaa taaaagagta aactaggaag tttatttcaa acaacctaaa
      961 atactgattt tcggctgaag ataatactgg agtgcaaatt aatggggtta taataaatag
     1021 ctgatagctt gtgttggttt tggatttttt aagagtagat gagtattaaa actataagga
     1081 ggacgaaggt ggctaaaaat ttaaaattaa aattagctcg ggtagagcgt gatttaacac
     1141 aaggtcaact ggcagaggct gtcggggtga cacgccagac tattggtttg atagaggcgg
     1201 ggaaatacaa tcccagtctc tcgctctgcc agtccatttg cagatgttta gggaaaaccc
     1261 tagaccaact attttgggag gaagaagatg aaaaatagat tttattatta tcaattacta
     1321 gacgaaagag aagaacaact gttcaataaa gcgggctctg aaagtttcta tatctgcatt
     1381 gctttgtcgc tcctatctta tatcatttca gtattagcac caagcctttt taattctaat
     1441 atgctgctaa tcgttatcat catagggaca ttttactttt tcaatcgtgc ccgttatctg
     1501 ggagtgacct actatagtcg ttttcatttt acgattttgg gttgtttttt cctaaccttg
     1561 gctattacgg ctcttttgat gttgcagaat tatcaattca acatagaaat ttatcagcac
     1621 aatcctttga attttaaata cctgtctgct tgggtcatta cttatgtcat ttaccttccg
     1681 tgggtcttta ttggcaatct tggtcttaag agctatggcg aatgggctca gaaaaaattt
     1741 gaacaagata tggatgaatt ggagagtgga gaatagcttg ttactctttt ctcaatccag
     1801 ctaaaatgtg atataatagt actaatttat tggaatacat gaaagttctt gaaaattttc
     1861 atgggtttct agctaaggaa gtaggaaaag tatgtatcca gatgatagtt tgacattgca
     1921 cacggacttg taccagatca acatgatgca ggtttacttt gaccaaggga ttcacaataa
     1981 gaaggcggtc tttgaggtgt atttccgcca acagcctttt aagaacggct atgcggtttt
     2041 tgcaggtttg gaaagaattg tgaactatct tgaagacttg cgtttttcag atagtgatat
     2101 agcctatttg gagtcgcttg gttatcatgg ggcgttcttg gattaccttc gcaatttcaa
     2161 gttggagttg accgttcgtt ctgcccaaga aggggatttg gtttttgcta atgaaccgat
     2221 tgtgcaggtg gaaggacctc tagcccaatg tcagttggtc gaaacggctc ttttgaacat
     2281 cgtcaactac cagaccttgg tggcgacgaa ggcagctcgt attcgttcgg ttatcgaaga
     2341 tgaacccttg atggagtttg ggacacgtcg ggctcaagaa atggatgcgg ccatctgggg
     2401 aacacgcgca gcggtgattg gtggcgccaa tggaaccagc aacgtgcgtg cgggtaagct
     2461 ctttgacatt cctgttttgg gaacccatgc ccatgccttg gtacaggttt atggaaatga
     2521 ctatgaagct ttcaaggctt acgctgcgac ccacaaaaat tgtgtctttc ttgtggatac
     2581 ctatgacacc cttcgcatcg gtgtaccagc tgccattcag gtggcgcgtg agctgggtga
     2641 tcagattaac tttatgggtg tgcggattga ctctggggat attgcctaca tttctaagaa
     2701 agtccgtcag caactggacg aggctggatt tacagaggct aagatttatg cttctaatga
     2761 tttggacgaa aatactatcc tcaatctcaa gatgcaaaag gccaagattg atgtctgggg
     2821 cgtgggtacc aagctgatta cagcttatga ccagccagct cttggtgcag tttacaagat
     2881 tgttgcaatc gaagatgaaa ctggtcagat gcgcaatacg attaagctgt ctaataatgc
     2941 tgaaaaagtt tctacgccag gtaagaagca ggtgtggcgc attaccagtc gtgaaaaagg
     3001 caagtcagaa ggcgactata tcacttatga tggtgtggat attagcgaca tgacagaaat
     3061 caagatgttc catccgacct atacatacat caagaagacg gttcgtaatt ttgatgccgt
     3121 tcctctcttg gtggatatct tcaaagaagg aatattagtt tacaacttgc ctagtttgac
     3181 tgacattcag gattatgccc gtaaggaatt tgacaagttg tgggatgagt ataagcgtgt
     3241 gctcaatccg cagcactatc cagtggattt ggcacgtgat gtatggcaag ataagatgga
     3301 cttgattgat aagatgcgca aggaagccct tggtgaagga gaagaagaat gagtttgcaa
     3361 gaaacgatta tccaagagct gggtgtcaaa ccagtgattg atgcccagga agaaatccgt
     3421 cgttctattg atttcttaaa aagatatctg aaaaaacatc ccttcctaaa aacctttgta
     3481 ctagggattt ctgggggaca agactcaacc ttggcaggac gtttggcgca attagctata
     3541 gaagaactgc gagctgaaac gggagacgac agctacaaat ttatcgctgt ccgcctgcca
     3601 tacggagtgc aagctgatga agcagatgct caaaaagccc tagccttcat ccagccagat
     3661 gtcagcttgg ttgtgaatat caaggagtca gctgatgcta tgacagctgc agttgaagcg
     3721 acaggtagtc ctgtttcaga cttcaacaag gggaatatca aggcacgttg ccgtatgatt
     3781 gctcagtatg cccttgctgg tttccatagc ggagcggtca ttggaacaga ccacgccgcg
     3841 gaaaatatca caggtttctt taccaagttt ggtgacggcg gtgcggatat tctccctctt
     3901 taccgcctca ataaacgcca aggaaaacag ctcttgcaga aacttggcgc agagccagcc
     3961 ctttatgaaa aaatcccaac agcagaccta gaagaagata aaccaggcct agctgacgaa
     4021 gtcgcacttg gagtcaccta cgcagagatt gacgactacc tagaaggcaa aacaatcagc
     4081 ccagaagctc aagcgaccat tgaaaactgg tggcacaaag gccaacacaa acgccactta
     4141 cccatcaccg tatttgatga cttttgggag taaaaaggtc cgggggacct ttttagcttc
     4201 ttgccctgaa attaaaaagc aagaaaaacc tctattggag gttttcagcc tctcatcttg
     4261 aaataagaaa gtgagagaag gtctggggga tcttgaaccc cgagtttaga aataagaaaa
     4321 tgaggcagat tcggtaactc gaagagttcg atttcatcgt cttacccctg caacgatgac
     4381 taggtttgaa aaagcttgct agagcgcatt tcaaaccagg cagcgactgc gtcaagaaat
     4441 tagaagacaa actcgttttc tagctgttac tgagttgagc ctttttacta cgagcataga
     4501 aataaggaag tgaggtagca tcatgaaatc tatcggtacg caaatattac agacagaacg
     4561 tttgatttta agaagatttg tggagagtga tgcagaagcc atgtttcaaa attgggcttc
     4621 atccgctgag aatctgatct atgttacctg ggatccccat cctgatgtcg aaatcactcg
     4681 aaactcgatt cgcaattggg ttgcttccta tactaatctc aactattaca aatgggccat
     4741 ttgtctaaaa gaaaacccag agcaagtgat aggagatatc agcatcgtta agatagacga
     4801 ggctgattta agctgtgaaa tcggctatgt gctaggcaag gcttactggg gacatggtat
     4861 gatgacagag gccttgaaag ctgtcttgga cttttgtttt actcaagcag gttttcaaaa
     4921 ggtcagagca cgttatgcca gtctcaaccc agcttcaggt cgtgtcatgg aaaaggctgg
     4981 aatgtcctat ctacaaacca ttgttaatgg tgtagagaga aaaggctatc ttgcggatct
     5041 tatttattat ggtataagta gggaagaatg ttgaattcta ttttctgttt ctatcgaagt
     5101 caactattta ttgtaaatat aataattagc attccaagtt tatttgaaac tttaaaatag
     5161 catattgatt agtacaagac agatgttcta gttccttctt taatctggtt tagtattagt
     5221 taaaaaatcg ctttaagctt gtaactaaga gggagctaat cgactagatt ctccagccga
     5281 acaggtggta atgtactttt tatagtgtaa tcctagctgt tgttaaattt aaaatagaat
     5341 cctctatcga gttagggaat taaattcaac caattttatt catgtttttt ctatcaaatt
     5401 atctaatatt aaaatagtct cattctgatg agaaaactat tctatttata ctgcatagtt
     5461 gtataaggaa tgatgaaaga tgagtgaaag ttatcaagtt ggaatgtttg tatctaaata
     5521 tattagcatg tatttagata agatgtctgc aatcctttat atatgagatt gattacgata
     5581 tagttaaagt gttagttcaa tagtaaatat atcattctaa gtctatttta tatataaagt
     5641 atattgttta acgtttgata gaaaagatgc tatactagaa gtaggatgta atggcaaaat
     5701 ctaatactaa taagcgctat gcaatacgta aactcggaaa gatttttggt tctgcggtaa
     5761 tcgggctaac tttattttta ggaggaatct gaatacaagt tttgttcatg ctgctgatgg
     5821 gattcaatat gtcagagatg atactagaga taaagaagag ggaatagagt atgatgacgc
     5881 tgacaatggg gatattattg taaaagtagc gactaaacct aaggtagtaa ccaagaaaat
     5941 ttcaagtacg cgaattcgtt atgaaaaaga tgaaacaaaa gaccgtagtg aaaatcctgt
     6001 tacaattgat ggagaggatg gctatgtaac tacgacaagg acctacgatg ttaatccaga
     6061 gactggttat gttaccgaac aggttactgt tgatagaaaa gaagccacgg atacagttat
     6121 caaagttcca gctaaaagca aggttgaaga agttcttgtt ccatttgcta ctaaatatga
     6181 agcagacaat gacctttctg caggacagga gcaagagatt actctaggaa agaatgggaa
     6241 aacagttaca acgataactt atgatgtaga tggaaagagt ggacaagtaa ctgagagtac
     6301 tttaagtcaa aaagaagact ctcaaacaag agttgttaaa aaaggaacca agccccaagt
     6361 tcttgtccaa gaaattccaa tcgaaacaga atatctcgat ggaccaactc ttgataaaag
     6421 tcaagaagta gaagaagtag gagaaattgg taaattactc ttactacaat ctatattgta
     6481 gatgaacgtg atggaacaat tgaagaaact acttctcgtc aaattactaa agagatggta
     6541 aaaagacgta taaggagagg gacgagagaa cctgaaaaag ttgttgttcc tgagcaatca
     6601 tctattcctt cgtatcctgt atctgttaca tctaaccaag gaacggatgt agcagtagaa
     6661 ccagctaaag cagttgctcc aacaacaggc tggaaacaag aaaatggtat gtggtatttt
     6721 tataatactg atggttccat ggcaacaggt tgggtacaag ttaatggttc atggtactac
     6781 ctcaacagca acggttctat gaaagtcaat caatggttcc aagttggtgg taaatggtat
     6841 tatgtaaata catcgggtga gttagcggtc aatacaagta tagatggcta tagagtcaat
     6901 gataatggtg aatgggtgcg ttaacagatt cctccctgcg acaaaattga taatagtatg
     6961 tctgaa
//
LOCUS       DAVKJD010000081         6867 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKJD010000081 DAVKJD010000000
VERSION     DAVKJD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.081"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            274..1117
                     /locus_tag="VW157_001743"
                     /pseudo
     CDS             274..1117
                     /locus_tag="VW157_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001857688.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            1294..2301
                     /gene="gap"
                     /locus_tag="VW157_001744"
     CDS             1294..2301
                     /gene="gap"
                     /locus_tag="VW157_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9553008.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2369..3205)
                     /locus_tag="VW157_001745"
     CDS             complement(2369..3205)
                     /locus_tag="VW157_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9553009.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3331..5526
                     /locus_tag="VW157_001746"
     CDS             3331..5526
                     /locus_tag="VW157_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9553010.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTSAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIANHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5579..5731
                     /gene="rpmG"
                     /locus_tag="VW157_001747"
     CDS             5579..5731
                     /gene="rpmG"
                     /locus_tag="VW157_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9553011.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5741..5917
                     /gene="secE"
                     /locus_tag="VW157_001748"
     CDS             5741..5917
                     /gene="secE"
                     /locus_tag="VW157_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9553012.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5972..6508
                     /gene="nusG"
                     /locus_tag="VW157_001749"
     CDS             5972..6508
                     /gene="nusG"
                     /locus_tag="VW157_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9553013.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
     gene            6630..>6867
                     /locus_tag="VW157_001750"
     CDS             6630..>6867
                     /locus_tag="VW157_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9553014.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKR"
BASE COUNT         2151 a         1179 c         1494 g         2043 t
ORIGIN      
        1 acctcaaaaa ggtattacca agttgcaata ccttttatga ggctcttttg tcctactctt
       61 gtttcaattg actatagttc acggttacag ttgggaaaga agctattaaa tatttggaga
      121 gtccttttgg gatgaacatg tcaaatatta ctatagatgg gcaagaaaag atgggcaaga
      181 aatggatctg agtcaaacga tgcttcttat gctcaagtag tgagtcttta tgatgataca
      241 agtatctctg ttagtaataa tgagacggac aaagtgttgg ctggatccct ctatactgat
      301 acaaatgaac agggattaac gattccaagc agtttactaa aaaattggaa tgaacagaca
      361 ggaaaaaatt tgacagctaa tgatcttatt ggcaaatcag tctcagccag cattgtagaa
      421 agtgctgccg aaactagtaa gattgctcaa tttcaaacga agattgtacg tgtaatcaat
      481 gatgaagatg acatggagga cagcaacagt ttcatgctgt ctcatcaaat ggaaacgatt
      541 ttgaaagagg ctggatttac gaaagctgta tcttatttta tcttatttta tcttggaact
      601 caaagatcca tcacagacaa aagtagtaac agaagaatta cagaaaaata agaagtatac
      661 tgtgctttct caacagagag ttcttgatat tgtgattacc tttattcgtg ttattcaggg
      721 attattgatt gtgctttcat cacaagctat tgtggtagca gcggttatga ttggtatcat
      781 tatttacatc aatatcatgc aacgttccaa ggaaataggt gtcatgaaag cagttggtta
      841 tcagaatcgt gatgtcaaag gaatttttat ttacgaggct atctggattg taggcatcgc
      901 cttgctgctg gcatttttgg ttgcacaagg ggtgggaagt ttggcgaatg cgattgtaag
      961 tcacttttac ccatccatca ctaaggtttt tgaattaaat cttttatctg ttttaggaac
     1021 tctagttttc gctctattac ttggttatgt ctcagcctac ttcccggcgc gtaagattag
     1081 taaaatggat cctgtagaat cgttacgcta tgaataataa gggattcctt gatttacagt
     1141 cttaaaaaga aactcacata gaaaaacaat ggccatactt gcaattcaac tcaaatagtt
     1201 gtataatagg tttgttgaaa ggtgatttgt agtgattcaa gttactcttt tcacaataaa
     1261 aatttcatga ttttcataag gaggaaatca ctaatggtag ttaaagttgg tattaacggt
     1321 ttcggacgta tcggtcgtct tgctttccgt cgtatccaaa acgtagaagg tgttgaagtt
     1381 acacgcatca acgaccttac agatccagtt atgcttgcac acttgttgaa atacgacaca
     1441 actcaaggtc gtttcgacgg tactgttgaa gttaaagaag gtggatttga agttaacggt
     1501 aaattcatca aagtttctgc tgaacgtgat ccagaacaaa tcgactgggc tactgacggt
     1561 gtagaaatcg ttcttgaagc tactggtttc tttgctaaga aagaagcagc tgaaaaacac
     1621 cttaaaggtg gagctaaaaa agttgttatc actgctcctg gtggaaacga cgttaaaaca
     1681 gttgtattca acactaacca cgacgttctt gacggtactg aaacagttat ctcaggtgct
     1741 tcatgtacta caaactgctt ggctccaatg gctaaagctc ttcaagacaa ctttggtgtt
     1801 gttgaaggat tgatgactac tatccacgct tacactggtg accaaatgat ccttgacgga
     1861 ccacaccgtg gtggtgacct tcgccgtgct cgcgctggtg ctgcaaacat cgttcctaac
     1921 tcaactggtg ctgcaaaagc tatcggtctt gtaatcccag aattgaatgg taaacttgat
     1981 ggatctgcac aacgcgttcc aactccaact ggatcagtta ctgaattggt agcagttctt
     2041 gaaaagaacg ttactgttga tgaagtgaac gcagctatga aagcagcttc aaacgaatca
     2101 tacggttaca cagaagatcc aatcgtatct tcagatatcg taggtatgtc ttacggttca
     2161 ttgtttgacg caactcaaac taaagttctt gacgttgacg gtaaacaatt ggttaaagtt
     2221 gtatcatggt acgacaacga aatgtcatac actgcacaac ttgttcgtac tcttgaatac
     2281 ttcgcaaaga ttgctaaata attcttgagt tgatagaaag caaggctttg tggtcttgct
     2341 tttttatatg gaaaaatgga tgacacgatc atccattctt ttttaattct ttttcaaatg
     2401 tatttgaaag ggtagtgaaa gttagcctct ctaaagtaag tgggtgggta aaggaaagtc
     2461 ggaaggcatg aagcataagc cggcttgtct ttgatttact attatagaga gggtctccca
     2521 ggataggaag attatgatgc aaaaggtgca cacgaatctg atgggttcgc cctgtcttta
     2581 gcttgcaatg agccaaggaa gtcttgtttg agaattgctt taatctgctt acatgcgttt
     2641 cagcatattg cccatttttt gcatcaacta ttctttttct acgatcatgg cgatcacgtc
     2701 caattttgtc tctgaaaaca agttcttttc tgttgatatt tccatcaact agagcccaat
     2761 attctctaga aatctctttt ttctccaata agcgattgag aatgggcagg ataaaaggat
     2821 ttttggcaaa gagaactaag ccactggttt ccatgtccag acgatgaacg acatagcagg
     2881 tttggccaac ataggtacta acatggttaa gaagggcaat ttcgtttggt tgattaccat
     2941 gcgttttcat accctctggt ttgtttacaa taatcaagtg ttgatcttga taaacttcct
     3001 gcactaagtc tgggttgccc caagggatcg tcttttgggg ataatcttcc tcgtcaaaag
     3061 tcaactggca aacatctcca ggatttacga tttcgttcca gtggacttct tcttgattta
     3121 tcaaaatatg tttcttgatt ctcaaaaaat gacggatttt tctagggatg aggagttgtt
     3181 cctcaagtaa ttgctttacc gtcatttgag gtagagaggc gggtaatgta aatgtgaatt
     3241 gcatacagat attgtaacaa aaaaagccct atttggatag gaaatagcta aattcttgtc
     3301 ttcctatgat gaagatgata aaataaacgc atgaaattag ataaattatt tgagaaattt
     3361 ctttctcttt ttaaaaaaga aacaagtgaa ctagaggact ctgattctac tatcttacgt
     3421 cgctctcgta gtgatcgaaa aaaattagcc caagtaggtc cgattcgaaa attctggcgt
     3481 cgttatcatc taacaaagat tatccttata ctaggtttga gtgcaggctt gctagttgga
     3541 atctatttgt ttgctgtagc caagtcgacc aatgtcaatg atttgcaaaa tgccttgaaa
     3601 actcggactc ttatttttga tcgtgaagaa aaagaggctg gtgccttgtc tggtcaaaag
     3661 ggaacctatg ttgagctgac tgatatcagt aaaaacttgc agaatgctgt tattgcgaca
     3721 gaagaccgtt ctttctataa aaatgacggg attaactatg gccgtttctt cttggctatt
     3781 gtcactgctg gacgttcagg tggtggctct accattaccc aacagctggc taaaaacgcc
     3841 tatttatcac aagatcaaac tgttgagaga aaagcgaaag aatttttcct tgccttagaa
     3901 ttaagcaaaa aatatagtaa ggagcaaatt ctaaccatgt accttaacaa cgcttatttt
     3961 ggaaatggtg tgtggggtgt agaagatgcg agtaagaaat actttggagt ttctgcatca
     4021 gaagtgagtc tggatcaagc tgcgactctg gcagggatgc tcaaggggcc ggaactgtat
     4081 aatcccttga attccgtaga agattctact aatcggcgcg atactgtctt gcagaatatg
     4141 gttgcagcag gatatattga taaaaaccaa gaaaccgaag ctgctgaagt tgatatgact
     4201 tcgcaattgc acgataagta tgaaggaaaa atctcagatt accgttaccc ctcttatttt
     4261 gatgcggtgg ttaatgaagc tgtttccaag tataatctaa cagaggaaga gattgtcaat
     4321 aatggctacc gcatttacac agagctggac caaaactacc aagcaaatat gcagattgtt
     4381 tatgaaaaca catcgctatt tccgagggca gaggatggaa cgtctgctca atcaggaagt
     4441 gtagctctcg aaccgaaaac agggggagtt cgtggagttg tcggtcaagt tgctgacaat
     4501 gataaaactg gattccggaa tttcaactat gcaacccaat caaagcgtag tcctggttct
     4561 acaattaagc ctttagttgt ttatacgcca gcagttgaag caggctgggc tttgaataag
     4621 cagttggata accataccat gcagtatgac agctataagg ttgataacta tgcagggatc
     4681 aaaacgagtc gagaagttcc tatgtatcaa gccttggcag aatcgcttaa tctacctgct
     4741 gttgccactg ttaatgattt gggtgtcgac aaggcttttg aggcaggcga aaaattcgga
     4801 ctcaacatgg aaaaggtcga ccgtgttctt ggtgtcgcct tgggaagcgg tgttgaaacc
     4861 aaccctcttc aaatggctca agcatacgct gcctttgcaa atgaaggttt aatgcctgaa
     4921 gctcatttta ttagtagaat tgaaaatgct agtggacaag ttattgcgaa tcataaaaat
     4981 tcacaaaaac gggtgattga taagtctgta gctgacaaga tgaccagtat gatgttgggg
     5041 actttcacca acggtaccgg tattagttca tcgcctgcag actatgtcat ggcagggaaa
     5101 actggaacaa ctgaagcagt tttcaatccg gagtacacaa gtgaccagtg ggtaattggt
     5161 tatactccgg atgtagtgat tagccactgg cttggctttc cgaccactga tgaaaatcac
     5221 tatctagctg gctctacttc aaacggtgca gctcatgtct ttagaaacat tgccaatacc
     5281 attttacctt atacgccagg aagtaccttt acggttgaaa atgcttataa gcaaaatgga
     5341 attgcaccag ccaatacaaa aagacaagta caaaccaatg ataatagcca gacagatgat
     5401 aatttgtctg atattcgagg gcgtgcgcaa agtctagtag atgaggctag ccgggctatc
     5461 tcagatgcga agattaagga aaaggctcaa acaatatggg attcgatagt caatctattt
     5521 cgctaagatg cttgtcaaag cctagctttc ttgttataat ggataagatg gaggcgttat
     5581 ggcactaaaa aaagcaagcc tagcttgtgc ggtttgtggt tcgagaaact attcaatcaa
     5641 gatcagcgga aaccccaagc ctacacgact agaagtaaat aaattttgta agcattgtgg
     5701 caagtacact acacacagag aaacgagata ggagagagcg atgcgtttta ttggagatat
     5761 ttttagactt cttaaagaca caacatggcc aactcgcaag gaaagctgga gagattttcg
     5821 ttctatcatg gaatacacag ctttctttgt agtaattatt tacatttttg accagttgat
     5881 tgtttcaggt ttgattcgat ttattaacat tttttagaag attagtggag ttaattacac
     5941 tagaaatctt ctatttatga aaggaaatat catggatagt tttgataaag gatggtttgt
     6001 tttacaaact tattctggtt atgaaaataa ggtgaaagaa aatctattac aacgtgcaca
     6061 aacctacaat atgttggata atattctacg cgttgaaatt ccaacacaaa cagtgcaagt
     6121 tgaaaaaaat ggaaagagaa aagaagtaga agaaaatcgc tttccaggtt atgttcttgt
     6181 agaaatggtc atgacagatg aagcttggtt tgttgttcga aacacaccaa atgttacagg
     6241 atttgtcgga tcacacggga acagatcaaa accaactcca ttattggaac aagaaattcg
     6301 tgacattttg gtatctatgg gacaaactgt tcaagaattt gatttcgatg ttgagattgg
     6361 tcaaactgta cgtattattg atggtgcttt tgcagactac actggtaaga ttacagaaat
     6421 tgataataac aaagtgaaaa tgattatctc tatgtttggt aatgacacag ttgcagaagt
     6481 aaacctaaac caaatcgcag aattataatc ctgagagagg ctggagcctc tcttttttgt
     6541 gcagtttagg agctaaaggg aacagaatgg agaaaatgga acaaatgtgt tttctaatct
     6601 gttagactgt atctagaaag gggaaaatta tgattaaaga attgtatgaa gaagtccaag
     6661 ggactgttta taagtgtaga aatgaatatt accttcattt atgggaattg tcggattggg
     6721 accaagaagg catgctctgc ttacatgaat tgattagtag agaagaagga ctggtagacg
     6781 atattccacg tttaaggaaa tatttcaaaa ccaagtttcg aaatcgaatt ttagactata
     6841 tccgtaagca ggaaagtcag aagcgta
//
LOCUS       DAVKJD010000082         6831 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKJD010000082 DAVKJD010000000
VERSION     DAVKJD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.082"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            3..6401
                     /locus_tag="VW157_001751"
     CDS             3..6401
                     /locus_tag="VW157_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9553015.1"
                     /translation="MSLTTAAVVLAAYVPNEVVLADTSSSEDALTISDTEKVVVDKEA
                     ENKEKHENIHNDIETSKDTEEKKTTVIEEKEVVSKESVIDNKTSNEEATIKEDSNQSQ
                     EDKANSSASKDPESPKKEDKLVYIAEFKDKESGEKAIKELSNLKDTNVLYTYNTIFNG
                     VSIETTPDNLDKIKLIEGISSVERSQKLQPMMNHARKEIGVEEAIDYLKSINAPFGKN
                     FDGRGMVISNIDTGTDYRHKAMRIDDDAKGSMRFKKEDLKGTDKNFWLSDKIPHAFNY
                     YNGGKITVEKADDGSDYFDPHGMHIAGILAGNDTEKDIKNFNGIDGIAPNAQIFSYKM
                     YSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAGIP
                     MVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFDKV
                     KIGGKNFKYRNIGAFFDKNKITTNEDGSKAPDKLKFVYIGKGQDQELIGLNLKGKIAV
                     MDRIYTKDLKNAFKKATDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEATKSQVFS
                     ISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKEID
                     FKFAPDTDRELYKEDIIVPAGSTSWGPRVDLLLKPDVSAPGKNIKSTLNVINGKSTYG
                     YMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLEGDDKIDLTSLTKIALQNTARPM
                     MDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNKDSKGLVNSYGSISLKEI
                     KGEEKYFTVKLHNTSDRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVPEI
                     HPEKIKGANITFEHDTFTIGANSSFDLNAVINVGEAKDKNKFVESFIHFESVEEMEAL
                     NSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSKSKTMEGYDDDGKPKIPGT
                     LNKGIGGEHGIDKFNPAAIIQNRKDKNITSLDQDPDLFAFNNQGIHAESTSGSNVARV
                     YPLDSKGEPQSVSLERGLTPSPLVLRSAEEGLISIVNINKEGENQRDLKVISREHFIR
                     GILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATRIRGQFE
                     PIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPRIISIDFSNPDKIKLIAKDTYHK
                     VKDEYKHETLFARDQKEHPEKFEEVANEVWYAGAALVDKYGDVEKNLDVTYAGEGEGR
                     NRKLDKDGNTIYEISGAGDLRGKIIEVIALDGASNFTKIHRIKFADKADKNGMISYYL
                     VDPDKDASSYKKLGEISEDKLKNAQSPEASNNVEEKKETVEEKPIEGPSTLELDKEIS
                     TVRNFENKDLKKLIKKKYKEEEDFVNGGKRKVELDYRYDTKGNITAYVDGSALEYETE
                     KLDDVKSKLGGVLSPSKDGHFEILGKVSNVSKNAKAYYGNGFKLIEIKASKYDPQTKT
                     LTFDLFANTNDVVDGLSFTGDMNILVKDKGKTKAKTKIRMPGKNRETKTEYPYASSYG
                     NVIELGEGDLSKNKPNNLTEMESGKIYSDSEKQQYLLKDNIILRKGYALKVTTYNPGK
                     TDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLMSALDGFN
                     IIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEAIKEDGSM
                     LFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVNESVVDNY
                     LIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYLQTGYSDL
                     NAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFDGEIQEQH
                     IYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSELKPHRVT
                     VTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYVINLSKDT
                     FIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREEHSQKSDST
                     KDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGLKKKNQD
                     "
BASE COUNT         2660 a          968 c         1359 g         1844 t
ORIGIN      
        1 tattgtcatt aaccacagct gcagttgttt tagcagcata tgtccctaat gaggtagtct
       61 tagcagacac atctagctct gaagatgctt taactatctc tgatacagaa aaagtagtag
      121 tagataagga agcagaaaat aaagaaaaac atgaaaatat tcataatgat atagaaactt
      181 ccaaggatac tgaagagaag aaaacaacgg ttattgagga aaaagaagtt gttagtaaag
      241 aatctgtgat agataacaaa actagcaatg aagaagcaac aatcaaagaa gattccaatc
      301 aatcccaaga agataaagct aactcgtctg caagtaaaga cccagaaagt cccaaaaaag
      361 aggataaact tgtctatatt gctgaattta aagataaaga atctggagaa aaagcaatca
      421 aggaattatc caatcttaag gatacaaacg ttttatatac ctataatacg atttttaatg
      481 gtgtttcaat agaaacaact ccagataatc tggataaaat taaactaata gaaggtattt
      541 catcggttga acggtcacaa aaactccaac ccatgatgaa tcatgcccga aaggaaattg
      601 gagttgagga ggctatcgat tacctaaagt ctatcaatgc tccatttggt aaaaattttg
      661 atggtagagg tatggtcatt tcaaatatcg ataccgggac agattatagg cataaggcca
      721 tgaggattga tgatgatgct aaaggctcaa tgagatttaa aaaagaagac ttaaaaggta
      781 ctgataaaaa tttctggttg agtgataaga ttcctcatgc tttcaattat tataatggtg
      841 gtaaaataac tgtagaaaaa gctgatgatg ggagcgatta ttttgatcca catgggatgc
      901 atattgcagg gattcttgcg gggaatgata ctgaaaaaga catcaagaac tttaatggaa
      961 tagatggaat tgctcctaat gcacagatat tctcttataa aatgtactct gacgcaggat
     1021 ctggcttcgc aggagatgaa acaatgtttc atgctattga agattcgatc aaacacaatg
     1081 tcgatgttgt ttcggtatca tctggcttta caggaacagg tcttgtaggt gagaaatatt
     1141 ggcaagctat tagagcatta agaaaagcag gcattccaat ggttgtagct acgggtaact
     1201 atgcgacttc tgcttcaagc tcttcatggg atttagtggc aaataatcat ctgaaaatga
     1261 cagatactgg aaatgtaacg cgaactgcag cacatgaaga tgcgatagcg gtcgcttctg
     1321 ctaaaaatca gacagttgag tttgataaag ttaagattgg aggaaaaaat tttaaataca
     1381 gaaatatagg ggcctttttc gataagaata aaatcacaac aaatgaagat ggttcaaaag
     1441 ctcctgataa attgaaattt gtatatatag gcaagggcca agaccaagag ttgataggtt
     1501 tgaatcttaa gggcaaaatt gcagtaatgg atagaattta tacaaaggat ttaaaaaatg
     1561 cttttaaaaa agctacggat aagggcgcac gcgccattat ggttgtaaat actgtcaatt
     1621 actacaatag agataattgg acagagcttc cagctatggg atatgaagcg gatgaagcta
     1681 ctaaaagtca agtgttttca atttcaggag atgatggtgt aaagctatgg aacatgatta
     1741 atcctgataa aaaaactgaa gtcaaaagaa ataataaaga agattttaaa gataagttgg
     1801 agcaatacta tccaattgat atggaaagtt ttaattccaa caaaccgaat gtaggtgacg
     1861 aaaaagagat tgactttaag tttgcacctg acacagacag agaactctat aaagaagata
     1921 tcatcgttcc agcaggatcc acatcttggg ggccaagagt agatttactt ttaaaacccg
     1981 atgtttcagc acctggtaaa aacattaaat ctactctcaa tgtcattaat gggaaatcaa
     2041 cttatggcta tatgtcagga actagtatgg caactccaat cgtagcagct tctactgttt
     2101 tgattagacc gaagttaaag gaaatgcttg aaagacctgt attgaaaaat cttgagggag
     2161 atgacaaaat agaccttaca agtcttacaa aaattgcttt acaaaatact gcgcgaccta
     2221 tgatggacgc aacgtcttgg aaagaaaaaa gccaatactt tgcatcacct agacaacagg
     2281 gagcagggct aattaatgtt gccaatgctt tgagaaatga agttgtagcg actttcaaaa
     2341 acaaggattc taaaggtttg gtaaactctt atggttctat ttctcttaaa gaaataaaag
     2401 gagaagaaaa atattttaca gttaagcttc acaatacatc agacagacct ttaaccttta
     2461 aagtttcagc atcagcgata actacagatt ctctaactga cagattaaaa ctggatgaaa
     2521 catataaaga tgaaaaatct ccagatggga agcaaattgt tccagaaatc catccagaaa
     2581 aaatcaaagg agcaaatatc acatttgagc atgatacttt cactataggc gcaaattcta
     2641 gctttgattt gaatgcggtt ataaatgttg gagaggctaa agacaaaaat aaatttgtag
     2701 aatcatttat tcattttgag tcagtggaag aaatggaagc tctaaactct aatgggaaga
     2761 aaataaactt ccaaccttct ttgtcgatgc ctctaatggg atttgctggg aattggaacc
     2821 acgaaccaat ccttgataaa tgggcttggg aagaaggatc aaaatcaaaa acaatggaag
     2881 gttatgatga tgatggtaaa ccgaaaattc ctggaacctt gaacaagggt attggtggag
     2941 aacatggtat agataaattt aatccagcag ctattattca aaacagaaaa gataaaaata
     3001 ttacatcact agaccaagac ccagacttat ttgcctttaa caaccaaggt attcatgcag
     3061 aatccactag tggctctaat gttgctaggg tatatccact tgactcaaag ggagaaccac
     3121 aaagtgtttc actagaaaga ggattaacac cttctccact tgtattaaga agtgcagaag
     3181 aaggattgat ttcaatagta aatataaata aagagggaga aaatcaaaga gacttaaaag
     3241 tcatttcgag agaacacttt attagaggga ttttaaattc taaaagcaat gatgcaaagg
     3301 gaatcaaatc atctaaacta aaagtttggg gtgacttgaa gtgggatgga ctcatctata
     3361 atcctagagg tagagaagaa aatgcaccag aaagtaagga taatcaagat cctgctacta
     3421 ggataagagg tcaatttgaa ccgattgcgg aaggtcaata tttctataaa ttcaaatata
     3481 gattaaccaa ggattatcca tggcaggttt cttatatacc agtaaaaatt gataatacag
     3541 ctccaagaat tatttcaatt gatttttcta atccagataa aattaaattg attgcaaaag
     3601 atacctacca caaggtaaag gatgagtaca aacacgaaac cttgtttgct agagaccaaa
     3661 aagaacatcc tgagaaattt gaagaagtag ccaacgaagt atggtatgca ggtgcagctc
     3721 ttgtcgataa gtatggagat gttgagaaaa atcttgatgt aacttacgcc ggagagggtg
     3781 agggaagaaa taggaaactt gacaaagacg gaaataccat ctacgaaatt agcggagcag
     3841 gagatttaag aggaaaaatc atcgaagtaa tagccctaga tggcgcaagc aactttacca
     3901 aaatccatag aattaaattt gctgataagg ctgacaaaaa tggaatgatt tcttattacc
     3961 tagtagatcc tgacaaagat gcttcttctt acaaaaaact aggggaaatt tctgaagata
     4021 aacttaaaaa tgcccaaagt ccagaagcct caaataacgt agaagaaaag aaagagactg
     4081 ttgaagaaaa acctatagag ggaccttcaa ctctagagtt agacaaagaa atctctactg
     4141 taagaaactt tgaaaataaa gacctaaaga aactcattaa aaagaaatat aaggaagaag
     4201 aagactttgt aaatggcgga aaaagaaaag tagagcttga ttatcgatat gatactaagg
     4261 gaaatataac tgcctatgtg gatggaagtg ctctagaata tgagactgag aaacttgatg
     4321 atgttaaatc aaagcttggt ggtgtattaa gcccatcaaa ggatggacat tttgaaattc
     4381 ttggaaaagt aagtaatgta tccaaaaatg ctaaggctta ctatggcaat ggcttcaaat
     4441 taatagaaat taaggcaagt aaatacgacc cacaaactaa aactctaacc tttgacttat
     4501 ttgcaaatac aaatgatgta gtcgatggtc tatcctttac gggtgatatg aatatccttg
     4561 taaaagataa gggtaaaaca aaagctaaaa ctaaaattag aatgcctgga aaaaataggg
     4621 aaactaaaac agaatatccc tatgcatcaa gttatgggaa tgtaatagaa ttaggagaag
     4681 gtgatctttc aaaaaacaaa ccaaacaatt taactgaaat ggaatctggt aaaatctatt
     4741 ctgattcaga aaaacaacaa tatctattaa aggataatat cattctgaga aaaggctatg
     4801 cactaaaagt gactacctat aatcctggaa aaacggatat gttagaagga aatggagtct
     4861 atagcaagga agatatagca aaaatacaaa aggccaatcc taatctaaga gtcctttcag
     4921 aaacaacaat ttatgctgat agtagaaatg ttgaagatgg aagaagtacc caagctgtat
     4981 taatgtcggc tttggacggc tttaacatta taaggtatca agtgtttaca tttaaaatga
     5041 acgataaagg ggaagctatc gataaagacg gaaatcttgt gacagattct tctaaacttg
     5101 tattatttgg taaggatgat aaagaataca ctggagagga taagtccaat gtagaagcta
     5161 taaaagaaga tggctccatg ttatttattg ataccaaacc agtaaacctt tcaatggata
     5221 agaactactt taatccatct aaatctaata aaatttatgt acgaaatcca gaattttatt
     5281 taagaggtaa gatttctgat aagggtggtt ttaactggga attgagagtt aatgaatcgg
     5341 ttgtagataa ttatttaatc tacggagatt tacacattga taacactaga gattttaata
     5401 ttaagctgaa tgttaaagac ggtgacatca tggactgggg aatgaaagac tataaagcaa
     5461 acggatttcc agataaggta acagatatgg atggaaatgt ttatcttcaa actggctata
     5521 gcgatttgaa tgctaaagca gttggagtcc actatcagtt tttatatgat aatgttaaac
     5581 ccgaagtaaa cattgatcct aagggaaata ctagtatcga atatgctgat ggaaaatctg
     5641 tagtctttaa catcaatgat aaaagaaata atggattcga tggtgagatt caagaacaac
     5701 atatttatgt aaatggaaaa gaatatacat catttgatga tattaaacaa ataacagaca
     5761 agacactaaa cattaagatt gttgtaaaag attttgcaag aaatacaacc gtaaaagaat
     5821 tcattttaaa taaagatacg ggagaggtaa gtgaattaaa acctcatagg gtaactgtga
     5881 ccattcaaaa tggaaaagaa atgagttcaa cgatagtgtc ggaagaagat tttattttac
     5941 ctgtttataa gggtgaatta gaaaaaggat accaatttga tggttgggaa atttctggtt
     6001 tcgaaggtaa aaaagacgct ggctatgtta ttaatctatc aaaagatacc tttataaaac
     6061 ctgtattcaa gaaaatagag gagaaaaagg aggaagaaaa taaacctact tttgatgtat
     6121 cgaaaaagaa agataaccca caagtaaacc atagtcaatt aaatgaaagt cacagaaaag
     6181 aggatttaca aagagaagag cattcacaaa aatctgattc aactaaggat gttacagcta
     6241 cagttcttga taaaaacaat atcagtagta aatcaactac taacaatcct aataagttgc
     6301 caaaaactgg aacagcaagc ggagcccaga cactattagc tgccggaata atgtttatag
     6361 taggaatttt tcttggattg aagaaaaaaa atcaagatta agataaaagc tatagaaaaa
     6421 aatggtttat gtactgagat tagatagtga ggtgatgaca tagttttgtg aaaatagcca
     6481 tttataactc aattatttag tttactttac tattgatact atttggagtt attaatggac
     6541 ttagtttaga taactaatga attgattgaa agggttagta ttgacaatat tggtcatatt
     6601 gattagagaa tagagtctat caaaatttaa aggctaatag aggtgatgag acaatttcaa
     6661 ggaattaagt agcgtttggt tgcttaattc ctttttattt ggaaggaagt ttttcgtaac
     6721 cgcccaataa cgaagtctat tgggcggtta cgatatgatc actgacttcg tcagtcttat
     6781 ctacaacctc aaaacagtgt tttgagcaac ctgcggctag cttcctagct t
//
LOCUS       DAVKJD010000083         6529 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKJD010000083 DAVKJD010000000
VERSION     DAVKJD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.083"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(117..1541)
                     /locus_tag="VW157_001752"
     CDS             complement(117..1541)
                     /locus_tag="VW157_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9553016.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VW157_001753"
     CDS             complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VW157_001753"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9553017.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLLTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VW157_001754"
     CDS             complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VW157_001754"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9553018.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
BASE COUNT         1896 a         1449 c         1195 g         1989 t
ORIGIN      
        1 tgtactattc tagtttcaat atactataat accaaaaagt tcgtattttt tctataatta
       61 ttattatttt atagcaaaaa aataaaacca ggaaaacaat ttcctgattt tacacgttat
      121 ttcacatcaa aaacaatgga tttgacattt gtcatcgctt caatgctata tttaatccct
      181 tgcactccag ctccagaacc tttgacacca aggaacggga aattatctgg accacgctgg
      241 gttttattat taatgtggac tgtacctact tcaagttttt cagcaatttc aaaggctttt
      301 ttgaaatcat ttgtaaagac tgatgattga aggccgaatt cagattcgtt ggcaaaggca
      361 atagcttcct ctacactagc cacacgaatg attggtaaaa caggaccaaa tggctcttcc
      421 catgccactt tcatatcttt tgtaacttgg tcaaaaagca ctggccagag aagattgccc
      481 tcacgtttga ttggtgtaag agcctgagcc tctttttctt gtgcatcctc aatcaagccc
      541 caaatgaagt cggctgaagc attgtcaata acaggtgtaa tatcagcatt gtcaaatgga
      601 tcaccaactg ttaatttaga aacttcttcc tgaagcaaag tagctaattt atctgctaca
      661 ctttcgagaa caatgacacg tttaatggcc gtgcaacgtt gtcctgagta gctaaaggct
      721 cctgcaacaa tttgcttggc agcatgttcc aaatctgcat cttctagtac aagagctgca
      781 tctttcccac caagttccaa catgatagga cgcataccag ctaaacgacc aatacgttct
      841 ccaatagggg ttgaacctgt aaagttgatg aagttgactt ctttgtgctc aatgatataa
      901 tccccaattt ctgaaccacg acctgtaatg gtgttgaaaa cacctgccgg aatccctgct
      961 tcttcaaatg ctttagccaa caagagtcca gaaatggaac cttgtgttgg tggcttaaac
     1021 atgaccacat tccctgcaat caaggcaggt gcaattttag aagcagataa attaactgga
     1081 taattaaagg gagcaatcgc tagcacgata ccaactggtt cacgacggac aacagccagt
     1141 ttgtttttac ttgctgcctc aaaaccacca ccttccattg cttgtccagt gatacggaga
     1201 ccttcctcag cagcataacg aatcaagtct gctgtacgca ctacttctcc aattgctgct
     1261 ttaatccctt ttgctacttc tttggcaagg atagtaccaa ttttttcctt atcgcgttct
     1321 aaaatagctg ctgttttatg caaataagcc gcacgttcaa ttgctgataa agctcgccat
     1381 gctggcaggg ctgcacgcgc agcttgcata gcctcatcag cttcagtctg agtcatggct
     1441 ggaactgtac ccaattcttc ttgattgatt ggtgaataaa tcgtaatttc ttgttcagat
     1501 gatttccatt ttccatttac taaattctga tatcttgtca aattcttgtc ctcctgaaaa
     1561 tgattaagat atattttacc aaattttcag aaaatcacaa ccctttttgc aaaatttttg
     1621 agattatttt cacaagcttg atttttcaaa gtatactcaa taaaaataaa aaaaatccac
     1681 tacatcaagg cgagactaat gtggtttgaa gaaattttcg aagagcgtga atgagtatca
     1741 tctatagtaa aataaaaaaa ctgaacaatt tggttgggga cagtcaaacc aatttctcac
     1801 aatgttttag aaacaagggt gtgctattcc aatttcaggc tactataact gtcatagatt
     1861 gctgaaacaa agtctaggta aaagtcttca taataaaaag accccctatc aagtgttcaa
     1921 aaactttgat aggaggtctt gttttgtgaa aatatttatc aaattttcta tacaagtgag
     1981 ctgttagcca ggttctttct attctttcaa tttcaatgaa tggatttttt accaatactc
     2041 ataactggga atttgtctgt gtaaaaatag cgagatagat ggtatttata aaacactcaa
     2101 gacagctaga ctaatatcat ttaaaacatt atcttctttt gagcgactgt tggttagcaa
     2161 catagctaaa tttcctgcat tttcaaattg atagggttct gatttagcat tcacaactac
     2221 caagaggtgt tctttgccgt gaacttcata gataaggcag ccgctatgtt caatcgcaga
     2281 atgcacaaag acatgatggt aaatttcatc atagctagag taagaaaagg caccagtttt
     2341 tgtcttcaat cggatgactt gacggataaa ctcaatactg tcttgacgct cattaatcaa
     2401 gttccagttc acttggttca cactgtcagg agcattatag ctattcatcg cacgctctct
     2461 atcatcatgg gtcaactcac cattttcacc agtcgcaacc agtttggtac gaccaaattc
     2521 ttgaccgatt tccataaagg ccatcccctg catgagcaga ttcatggctg tggcagtttc
     2581 gaccttgcgc atgatttgct ctgaactttg gtctggatga agggttgcca ataaatcgtg
     2641 aagattgtaa ttgtcatggg cttctacata gttaagcacc tgatttggat gtgtatagct
     2701 tcctaattca cgacttccta ggattgcttt agctagaatt ggctctgtcg cagcaccact
     2761 gacaaaacct gacttgatag caccataaac ttctccccct ttgacagcat cgcgctgatt
     2821 gtcattaaag aaaccaatat ttggcatctg gtaggcattg tccttcttgg ccttatcata
     2881 aggggcaaga cctgttccca tatcccatcc ttctccatag aggataatgt tggagtcgat
     2941 ttcatccaag ctttgacgaa tcatctgcat ggtcttgaca tcatgaatcc ccatcaagtc
     3001 aaaacggaag ccgtcaatat tatattcctg cacccagtat agaagagaat caatcatata
     3061 cttgcgaaac atttcgtgtt cactggctgt ttcatttcca acacccgttc cattctggaa
     3121 ggtaccatct ggattcatac gataatagta atcagggact gttgtttgga atggtgcatc
     3181 aacaactgag aaggtatggt tatagactac atccataatg actccaatac ccgcatcgtg
     3241 ataagcttga accatcacct tcaaatcacg aatgacctga gctggatcat ctggattagt
     3301 tgaaaaacta gtttctggcg cgttatagtt ttgtggatca taaccccagt tgtaggttac
     3361 atttccatcc tcatcgtatt ctttatgacg gtctgcaatt ggttgcaatt gaacataatt
     3421 gtagcccagc ttcttgatgt aatcaaaagc agttgactgg ccgtattggt taactgttcc
     3481 agcctgagca gcacccaaga aagttcctcg aagatgttca tctacacccg atgtaggtga
     3541 tttagtcaaa tcacgaatgt gcatttcaca gataactgcc ttacatggat tttccaagcg
     3601 ccaagtagcc tccgaaccgt gcttaacctc gaagttttca acttgctttt ctacatggct
     3661 cagaatagct gaacgtttgc catcagggct ggtcgcgatt gtataaggat cacgtgtcag
     3721 tgtttggtga tgagggaatt ggacttgata ctgataagtc ttacctacca aatcttcttc
     3781 aacatccaaa ctccagacac cgattgtatt gtccttatga ttataagagt agctattgcc
     3841 tcttttcatc tcaaaagtct tccaaacggg tgcatcatta gcagctgatt cataaacgac
     3901 aacttgcact tctgtcgctg taggtgacca gagggaaaaa tgagcctgat tgtcctctac
     3961 acggcaaccc aattctcctt ggtaacccca atgatgatca aaactagcac tgttaatggc
     4021 cttatcaaag gcaaaaggat tttgattttt atagaaagga ctggcaatag caggattttc
     4081 agagtaataa atcctatcat cgccttccaa aatccagacc tctgttaata ggggatagtg
     4141 attaaaacgg atagaatatt ctttactagt ttgacctgta tgaaccacaa aattcaagct
     4201 ttctataaca tgtgaacttg ggtgttcaaa gctaaataaa gctccaaaat aatcttcttt
     4261 gtaggttagc aaatcaattc gttgatcctg actttttaca aaggagcaag tgtcatattc
     4321 tccattctta cgatggtaat gaatgcgcat agggtagtta tacatttttt atttttcctt
     4381 tttactttgt ttctatttca ctaataaatt tttgtcaatc tcgtctcaat taacagacat
     4441 agtcatattc tctaaactct gtttttaaac gatccattac aaactttcta gccatgcctc
     4501 atctctgacc tggataccaa gttcttgtgc tttttgcagt ttacttccag cgtctgcacc
     4561 taccacgacg aggtcggtct ttttagaaat actacctgtc actttggcac ccagactttc
     4621 gagtttactt ttagcttctg agcgcttgag tcgttccaat tttcctgtca ataccacggt
     4681 caaacctgac aaggccgcat ccgctactac cgtctgtcct ttatagtcca gattgacccc
     4741 agtttctttc aattctctga gcagaatttc agagccttct gtcgcaaaat aagtctgaag
     4801 acttttggca atcacgccac ctagactttc aatactagcc acttcctctg aatctgcctg
     4861 agacagattt tcaattgaat ggaaatattg aagtaaaagc tgactaacct tgcttccgac
     4921 atgacgaatt cccaaaccaa ataagagctt ctcggcagaa ttttcctttg atgcttggat
     4981 agcctgatac agtttagcag cggacttttc cttaaccccc tctaaaagga ggaaatcctc
     5041 ttcttgcaaa cgataaatat ccgccacatc cttgactaaa ttagcagcaa aaagcttctc
     5101 aacaatagat ggaccaaggc ctgtaatatt catagcatca cgagaagcaa agtgaatcaa
     5161 gccttccatg atttgagcag ggcaacgcgg attgatacaa cgtagggcca cttcatcttc
     5221 aaagtgcaac aagtcagagt tacaacttgg acagtttgta gggatatcta gtttttcttc
     5281 agaaacccgt ttggactcta ccacacgtaa aacggcaggg atgatgtcac cagccttata
     5341 tacaatgacc gtatcgtctt ttcggatatc tttttcagca atataatcta cattgtgcag
     5401 ggtcgcacgg ctaacagtcg taccggcaag ttgtactggt gttagattag cagttggagt
     5461 tacaacaccg gtacggccaa ctgtccagtc aactgataag agttgagctt ctttttcttc
     5521 agcagggaac ttgtaggcta ctgcccactt tggagcctta actgtaaaac caagttcttc
     5581 ttgacttgct aggtcgttga ccttgattac cactccatca atatcgtaag gcagattttc
     5641 ccgttcctgt cctacttctt ggataaaatt ccagatttca tctatgtttt cagccaagat
     5701 tcgcttagga ttgaccacaa aacctagttg ttctaggtac ttcaaaccct tttcttggct
     5761 atcacgagtt gaagggctgg cttcttgata gagaaacgtt gcaagattac gcttggcaac
     5821 tactgctgta tccaactgac gcagagttcc tgctgccgca ttacgaggat tagcaaattc
     5881 aggctctcca ttttcttggc gcgcttggtt aacttggtca aaggaagcgc gtggcatgta
     5941 acattcccca cgaactgtga tatctagttc ttctggcaaa gtcaaaggga tgtccttaac
     6001 acgcttgagg ttttctgtga tattttcacc aattgaacca tctccacgtg ttaccccagc
     6061 aaccaaaatc cccttttcat aagtcagcga gatagataag ccatcgattt tcaactcaca
     6121 aatataggtc ggatgagcca cttccttacg aacacgcgca tcaaaagcat ctagctcctc
     6181 acgtgaaaaa gcatcctgca aactataaag aggatactga tgactgtatt tttcaaaacc
     6241 atctaaaacc ttgccaccaa cacgatgagt cggactgtct gctagcactt gctctggata
     6301 agcagtttct aactcgacca actcacggta aaggcggtca tactcactgt ctgaaaccga
     6361 gggattatcg ctggtatagt actcagtcgc atagcgattg agcaaagcga ctaactcatt
     6421 cattctttta ttcataagac cattttacca taaaacaagc cctcctcaca aacgagaagg
     6481 gcggaaaaaa cacttagttt gaaattattt ttgaaactca agcaacctt
//
LOCUS       DAVKJD010000084         6507 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKJD010000084 DAVKJD010000000
VERSION     DAVKJD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.084"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            1..611
                     /locus_tag="VW157_001755"
                     /pseudo
     CDS             1..611
                     /locus_tag="VW157_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            complement(845..1141)
                     /locus_tag="VW157_001756"
     CDS             complement(845..1141)
                     /locus_tag="VW157_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553019.1"
                     /translation="MLQSPYAIDTINLKKDFLEKPIDIEKFKAFLEKEEIPLAIAWQG
                     DSLHFYTKDRSILDNHLDHLLEKMVNDPEKLSDFSMDKSLDDAIDEVKSQITFK"
     gene            complement(1154..1297)
                     /locus_tag="VW157_001757"
     CDS             complement(1154..1297)
                     /locus_tag="VW157_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553020.1"
                     /translation="MSSEQQERMAVQYAEPSLLFTVRSLLKMLEWSRRQALEQDSAYK
                     IGV"
     gene            complement(1299..1586)
                     /locus_tag="VW157_001758"
     CDS             complement(1299..1586)
                     /locus_tag="VW157_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553021.1"
                     /translation="MKVVNLYDLKQMGNKGGCTIQLIHHFPFGMGLGHLKKDYIEFKR
                     VGIFDGKAVEVTLREPYSRDLLQVVKSIKQRQKLIAYRYKEGKLLFVKKEV"
     gene            complement(2181..2291)
                     /locus_tag="VW157_001759"
     CDS             complement(2181..2291)
                     /locus_tag="VW157_001759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9553022.1"
                     /translation="MKLLLDMKNENRLIIIATHNPDIWNQADEVINLNQL"
     gene            complement(2388..2591)
                     /locus_tag="VW157_001760"
     CDS             complement(2388..2591)
                     /locus_tag="VW157_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9553023.1"
                     /translation="MTVSGYLFQNFGLLDNENVSTDLDLGLISRKCNFKEKDKLKKDV
                     LARVGLTHIHLEQKIYELSGGEA"
     gene            complement(2667..3278)
                     /locus_tag="VW157_001761"
     CDS             complement(2667..3278)
                     /locus_tag="VW157_001761"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEU9553024.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKAFAVAKSLKNSLMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIDKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
     gene            complement(3301..4686)
                     /locus_tag="VW157_001762"
     CDS             complement(3301..4686)
                     /locus_tag="VW157_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEU9553025.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVKFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            complement(4687..6462)
                     /locus_tag="VW157_001763"
     CDS             complement(4687..6462)
                     /locus_tag="VW157_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEU9553026.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTISIGQKVSTGDILGTVKETEVVNHKIMVPYGISGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQALGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDDKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSENERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIIEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIQLILETGGL"
BASE COUNT         2037 a         1339 c         1020 g         2111 t
ORIGIN      
        1 tatagaactt ttagactgta atcaaacaac gatttggcga aatgtaaaaa aatatgagga
       61 gttcggactc gactccctcc ttcaagaaac acgtggtggt cgtaaccatg catatatgac
      121 agttgaggaa gagaaagtct ttcttgcccg ccatttgaag gctgcagagg caggagaatt
      181 tgttacaatt gatgccttat ttcaggctta taaaaaggag ttaggtcgtt cctacacacg
      241 tgatgccttc tatcaactgt tgaagcgcca tggttggcga aatattacgc cacgtccaga
      301 acatcctatc aagaagaaaa gaaagcgctt taaaaccagt agatgttttc ataaggttcg
      361 cttgatgtac caagatgaag ctggtttcgg tagaatcagt aaactgggat cttgttgggc
      421 tccaatagga gtaggtccac atgtccatag tcactatata cgagaatttc gctattgtta
      481 tggagctgtt gatgcccata ttggaagatg tcatgaatca actccaagat gtcatacaag
      541 gactggagaa ggaggtgata aagtccatcg ttaatcggag atggactaga atgctttttg
      601 aaaacagatg agtattagaa ataaatgaaa agtcaatatc aaaacaatca gaatcttgat
      661 gatgctaaat tcaaaaaaac tacataataa ttttaataag ggtaagtcgt accaagaaac
      721 cccaccaaga taaaaactta gtggggtttg gcggggaata taattattct cccaattatt
      781 atcgccttta tattatctca actctcatta aacaagagtt ccgcaatcaa attgcgatac
      841 tctattattt aaaggtaatt tgggatttaa cctcatcaat tgcatcgtct aatgacttat
      901 ccatggaaaa atcagataat ttctcaggat cattcaccat tttttctaac agatggtcta
      961 aatgattgtc tagaatcgaa cggtctttcg tgtagaaatg cagggaatcc ccttgccaag
     1021 cgatggctaa aggaatctct tctttttcta aaaaagcttt aaatttctct atatcaattg
     1081 gtttttctaa aaaatctttt ttcagattaa tcgtatcaat cgcataggga gattgtagca
     1141 attcttctaa tttctacacc cctatcttat aggcggaatc ctgttctaaa gcctgacgtc
     1201 tagaccattc tagcatcttt aagagacttc ttacagtaaa taaaagacta ggctctgcat
     1261 attgaactgc cattcgttcc tgttgttcag aggacatctc agacctcctt cttaacaaat
     1321 agcagctttc cttctttata acgataagct atcaatttct gacgttgctt aatcgacttg
     1381 acaacctgca gtagatctct cgaataaggt tctcgaagag taacttctac tgctttccca
     1441 tcaaagatac caacacgttt aaattcaatg tagtcttttt tgagatgtcc taagcccata
     1501 ccaaagggaa agtgatgaat caattggatg gtacaaccac ctttatttcc catttgtttc
     1561 aaatcataca agtttactac cttcatgatt aactccttta tctagtttgt cgcatcgttt
     1621 gagtctggaa acgataaact ccgtgtctgt tagaaaattc tcacacacgt cttgtgccag
     1681 ttgcccttca cagggaaata ctctcagtcc ctacttacac aggcacgcta atcaagacgg
     1741 agtgaattca attttcaaag aacaggtagc tttattatag ataagagtag ttgaaatttt
     1801 tatcacatct ttgaggttat tggaataatc aggtaagtgc taatttctag ctcttctcac
     1861 aacaccgtac ttacctttta tgttttatga acagtagcta tcatttacat tttcaaacta
     1921 ccattctatc agactaaaag tcaaaataga cgcttctact tcgagatata ctcatttaat
     1981 ggttcagcaa ttcgctctac ttctgttaca gaaatttcat cactttgact tctcatgatt
     2041 tgttattagt tcactttgag ggcttgctca tgaagccaaa cagcgtaagt cgtaaaagaa
     2101 aaaagcagta caagattgaa ctgcttttac ggattcagat aaaataactt taaaatatag
     2161 aaaatatata ttggctgtaa ctataattga tttaaattta taacttcatc tgcttgattc
     2221 caaatatcgg gattatgagt tgcaatgatg attagacgat tttcattttt catatctaat
     2281 aataatttca taattccttg tgaagtcgtc ggatcgagtg atgcagtcag ctcatccgcg
     2341 agtattaatg gtggattttt aagaatgatt ttcgctaaag caacacgtta tgcttcaccg
     2401 ccagacaatt catatatctt ttgttctaga tgaatatgag ttagaccaac tcttgctaat
     2461 acatcttttt ttaacttatc tttttcttta aaattacatt tacgacttat taatcctaaa
     2521 tctaaatcgg tagaaacatt ttcattatct aacaaaccaa aattttgaaa aagatatcct
     2581 gatactgtca ataaaaaagc ttagaaaggc ttaccgcctt atccaagctg atattggttc
     2641 atctaattaa aatctaaata aaggatctac ttcactttca taatccgaac taaattggct
     2701 ctttctgcct cctctagttt caattctata taatgaatag tttctgacaa gtttggaata
     2761 atcgaatatt ctaatccatt tacacgtcta cgtgtttttt ctatttcatc agccattaac
     2821 tgacacgttt tttcaacttc tgccagtctt agtaatttat caattaaact attcattgta
     2881 gcaaatacat catccatttc actattagaa gataaatagc tgtattcact gttctcattt
     2941 tgagaagtaa tattcatatg cattctagga actgttacac tcatgatatt ttctttctca
     3001 acaaataatt caatttcttt cgatggaatt gaaaataatt cctccaccat tagagagttc
     3061 tttaatgatt tagcaactgc aaaggctttt agattatcaa ttagataact ttctacttct
     3121 ttccgaagtt gattattctc acgaatcaaa gaaataaatc gcctcatcaa ttcatctctt
     3181 ttatccttta ataacttatg tccacgttca gctgttttca aacgttcctt taagttattc
     3241 aattccatac gagttggttt tacattcaaa cgcaccatag ataatcactc catttctgga
     3301 ttaaacttct accaaaggta agtatttatc aagcaaatca tctttgatac gttttaactc
     3361 tgttctagga agaattgata gtaattccca cccaagattc aacgtatctt caatatttcg
     3421 atttttataa aatccttggt tgatgtactc ttcttcaaaa cgctttgtaa acttcacata
     3481 caatttatct acatcagata aagccgattc tcctaatact actgctaact cttcaacctt
     3541 tttcccttgg gcataggctg caaacagttg attcatagtt ggagcatgat ctccacgagt
     3601 tttaccttct ccagatccct tatcttttaa tcgagagaga gaaggtaaaa cattgattgg
     3661 tggacgataa ccttgattat acaactcatg cgacaaaata atttgccctt cagtaatgta
     3721 tccagttaaa tcaggaattg gatgtgttat gtcatcttct ggcattgtta aaataggaat
     3781 ctgtgtcact gaaccttttt taccaactaa gcgaccagcc ctttcgtata gagttgataa
     3841 atttgtatat aaatatcccg gatagcctcg tctacctgga acttcacggc gagctgccga
     3901 gacttcacgt aacgcttcac aatagttagt catatccgtc atgataacta gaacatgcat
     3961 atctttttca aaagctagat actctgccgc agttaaagca atgcggggag ttgcaatacg
     4021 ctcaattgca ggatcatttg ccaagttcat aaataagact gaacgatcga tcgctcctgt
     4081 ttttctgagt tcttccataa agaactcagc ttcttcaaaa gtaataccca ttgctgcaaa
     4141 tacaaccgca aaattttcat cagaatttaa aacagtcgct tgtcttgcta tctgagctgc
     4201 taattcatta tgaggtaagc ccgaacctga aaatactggt aatttttgac cacgtacaag
     4261 agtattcaaa tgatcaatag aggagatccc tgtctgaata aattcatctg gataatctct
     4321 agatacagga ttaatagctt gaccatcaat atctaaatat ttctctggaa ttaaatctgg
     4381 tccaccatca attggttttc ccatcccatt aaaaattcta ccaaccatat cctcagatac
     4441 agccaattct agtgcatgac cagcaaaacg aattttagac ttttctaaat ttattccact
     4501 agatccttca aaaagctgaa ccattgcttt atcttcgtgg atctctaaaa cttgtccacg
     4561 acgaatttct ccattatgaa gttgaatttc aactaactca ttgtaagaca cattattaac
     4621 ttgttcaaca atcataagag gcccaacaac ttcactagca gttctatatt cttttataac
     4681 actcatttat agacctcctg tttctaatat caattgaatt tgatgtgtaa tctcatttga
     4741 aataattttg atttcatcta atctatcttc tgaaacatat ttacttctcg ccatacggtc
     4801 tcgaactgcc acggtacctt ctataatctc tgtaaagtaa gaacccaact ctaaagcatg
     4861 atttgcctga tcagcaaaag ttaggatatt acttaacatt gcttcttgtt ttgcaaacga
     4921 agtgaatgta tctaccgaat caaaagcgtt ctgttgcaaa taatcttctc taatttgttt
     4981 agcaatttcc atcgttagtc gttcattttc agacagagaa tcaattccaa caagacgaac
     5041 aatttcctct aaactagatt cccgttgtaa gtagttcatc gcacgagtta ttttactatt
     5101 ccaatctgtc ttctctttat catctatata agttcccaca ctgtctttat agagtgaata
     5161 agaggtaagc cagttaattg caggaaaatg acgtcgctgt gccaacggag catcaagccc
     5221 ccaaaaaact ttcacaatcc gtaaagtgtt ttgagtaact ggttctgaaa tatctccacc
     5281 gggtggcgat acagctccaa tagcagtaat cgttccttca cgttctggaa gccctagagc
     5341 ctgagaacgt cccgctcttt cataatattc agcgatacga cttccaagat aagcaggata
     5401 accctcatca ccaggcattt cttctagacg tcctgacatt tcacgtagcg cttctgccca
     5461 acgtgaagtc gaatcagcca taatggcgac agagtagccc atatcacgaa aatactcagc
     5521 catggtaatt cctgtataaa ttgaagcctc acgagcagca acaggcatat ttgaagtatt
     5581 agcaatcaga actgtccgtt gcataattga ttgtcctgta ttagggtcaa tcaactcagg
     5641 aaactcattc agtacatccg tcatttcatt tccacgttct ccacaaccga cataaataac
     5701 aatatcaaca ttggcaaatt tagctacttg gtgttgtaca actgtctttc ctgctccaaa
     5761 cggtccagga acagctgctg ctcccccttt ggttactgga aagaatgtat caataactcg
     5821 ttgacctgtg attaatggtt cttctgggat taaacgttta gaaacaggac gcgccttgcg
     5881 gacaggccat ttttgcataa gtgttccttt atagaaacta ccgtccaatt tttttatttc
     5941 atatacaact tcatcaattg taaaatcgcc agaggcaata gaaacgactt cgccagatat
     6001 tccataagga accataattt tatgattaac tacttcggtt tccttgacag ttccaagaat
     6061 atcacccgta ctcacttttt gaccaattga tatagtggaa tcaaaatgcc acttaatatc
     6121 tctatccaaa cttggaactt ctaccccacg aactagaaaa tcattatgag tagccaattt
     6181 aaatcgatct aatgggcgtt gtatgccatc aaacatttga gaaatcaatc caggccctaa
     6241 ttcaaccgag agaggttctc cagttgtaac aacaggttct cccggaccaa gaccagatgt
     6301 ttcttcatag acttggatag atgcctgatc tcttctcatt tcaataattt caccgattaa
     6361 ccctagctta cctacacggc aaatatcttg aatattagcc tcctgcatac ctgatgcaat
     6421 aactagaggt cccgatactt ttataatctt cccttgagtc aaagttattc tccttactaa
     6481 gtccaaaaat tatagaataa tattaca
//
LOCUS       DAVKJD010000085         6114 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKJD010000085 DAVKJD010000000
VERSION     DAVKJD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.085"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..380
                     /locus_tag="VW157_001764"
     CDS             <1..380
                     /locus_tag="VW157_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9553027.1"
                     /translation="NTNLTDLVDGKIDIDGENVFANCMTYLADGVPGDIFETHKKYLD
                     IHIVVENTEKMAVTSPVRAQSRVPFSEEKDIAFYDSKDYQIVELLPGNMLVTFEEDLH
                     QPKIHCNDETVKKLVIKVLNEEK"
     gene            382..2604
                     /locus_tag="VW157_001765"
     CDS             382..2604
                     /locus_tag="VW157_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU9553028.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPV
                     APTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     KELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            2644..3753
                     /locus_tag="VW157_001766"
     CDS             2644..3753
                     /locus_tag="VW157_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9553029.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            3910..4800
                     /locus_tag="VW157_001767"
     CDS             3910..4800
                     /locus_tag="VW157_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9553030.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            4825..5148
                     /locus_tag="VW157_001768"
     CDS             4825..5148
                     /locus_tag="VW157_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553031.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENDANIQYISSSRRGEFVEKIIGRVV
                     EKYGH"
     gene            5138..>6114
                     /locus_tag="VW157_001769"
     CDS             5138..>6114
                     /locus_tag="VW157_001769"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEU9553032.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYNSLLENNIEVQEIFSNDREYGVVVFY
                     QSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKYLID
                     LQWQIDYILSIYARQISKNNFLCTPHLVALEGWIEETRILYFIKVMDEH"
BASE COUNT         2169 a          858 c         1227 g         1860 t
ORIGIN      
        1 aaaatacaaa tttgacagat ttagttgatg gaaaaattga tattgacggg gaaaatgttt
       61 ttgcaaattg tatgacttat ctagctgatg gagttccagg agatattttt gaaactcata
      121 agaaatattt agatattcat attgttgttg aaaatactga aaaaatggca gtaacttccc
      181 cagttcgtgc ccagtcacgt gtacctttta gcgaagagaa ggatattgct ttttacgata
      241 gcaaagacta tcaaattgtt gaattgcttc cagggaatat gttggtgact tttgaagaag
      301 atcttcacca accaaagatt cattgcaatg atgaaactgt taaaaaactt gttatcaaag
      361 ttttaaacga ggaaaaataa aatgaaaaaa aatattaaac aatatgtaac cttaggtact
      421 gtagtggtat tatcagcatt tgttgctaac tcagttgcag ctcaggagac tgaaacttct
      481 gaagtatcaa caccaaagtt ggtgcaacct gttgcaccaa cgactccgat ttcggaagta
      541 caacctacat cggataactc ttcggaagtt actgtacaac ctcgaacagt tgaaactact
      601 gttaaggatc catcttctac agcggaagaa actcctgtct tagaaaaaaa taatgttact
      661 ttaacagggg gcggagaaaa tgttactaaa gagttaaagg ataaatttac tagcggtgac
      721 tttactgtag tgattaagta caatcagtca agtgagaaag gcttacaagc tctgtttgga
      781 atatctaatt ccaaacccgg tcaacaaaat agttatgtag atgtgttcct tagagacaat
      841 ggtgagttgg ggatggaagc gcgtgatact tcttccaata aaaataacct agtatccaga
      901 cctgcttcag tttggggtaa gtacaaacaa gaggctgtga ctaacactgt tgcagtagta
      961 gcagattcag tcaaaaaaac atattcttta tacgcaaatg gtacaaaagt agtagaaaag
     1021 aaagtggata atttcctaaa catcaaggat attaaaggta ttgattacta tatgcttggg
     1081 ggagtgaaac gtgcaggaaa aacggcgttt ggttttaacg gaacactaga aaatatcaaa
     1141 ttctttaata gtgcattgga tgaagaaact gttaaaaaga tgacaacaaa cgctgttact
     1201 ggacatttaa tttatacggc taatgataca acaggttcta actatttccg tattccagtt
     1261 ctgtatactt ttagcaatgg tcgggtattt tcaagcattg acgctcgtta cggtggaact
     1321 catgatttct tgaataaaat taatattgct acaagttata gtgatgataa tggtaagaca
     1381 tggactaaac caaaattaac attggcattc gatgattttg cgccagtacc attagaatgg
     1441 cctcgtgaag ttggtggacg tgacttacaa atcagcggtg gtgcaaccta tattgactct
     1501 gttattgttg aaaaaaagaa caaacaagta ctcatgtttg ctgatgtgat gcctgctgga
     1561 gtaagtttta gagaagcaac tagaaaagat tcaggttata aacaaattga tggtaattat
     1621 taccttaaat taaggaaaca aggtgatact gattacaatt atactattcg tgagaatggt
     1681 actgtatacg acgatcgtac caacagacca actgaatttt cagtagataa aaatttcggt
     1741 attaaacaaa atggtaatta tttgacggta gagcaatatt cggtttcatt tgaaaataat
     1801 aaaaagactg aatatcgtaa tgggactaaa gtccatatga atatcttcta caaagatgca
     1861 ttgtttaaag tagtgccaac gaactatatt gcatatattt ctagcaatga tcatggcgaa
     1921 tcttggtctg caccaacttt attacctcct ataatgggac ttaatcggaa tgcgccatat
     1981 ttaggtcctg gacgtggaat cattgaaagc tcaactggac gtattcttat tccgtcttac
     2041 actggtaaag agtctgcgtt catttatagt gacgataatg gagcatcttg gaaagttaaa
     2101 gtagtgccac ttccttctag ttggtcagca gaagcacaat ttgtagaatt gagtccagga
     2161 gtaattcaag catatatgcg tacaaataat ggtaaaattg catatttaac aagtaaagac
     2221 gcaggtacta cttggagtgc accggaatat ttgaaatttg tttcaaatcc aagttatgga
     2281 acacaattat caatcatcaa ttatagccaa ttgattgatg gtaaaaaggc tgtcatttta
     2341 agtactccaa actccacaaa tggtcgtaaa cacggacaaa tttggattgg tctaattaat
     2401 gatgataata caattgattg gcgttatcat cacgacgttg attatagtaa ctatggatac
     2461 tcatattcaa cattgacaga gttaccaaat catgaaattg gattgatgtt tgaaaaattt
     2521 gattcatggt ctcgtaatga acttcatatg aaaaatgttg taccatatat aacatttaag
     2581 attgaagatc tgaaaaagaa ttaaagctga aatttgaaaa tatataaaaa gaggataaaa
     2641 attatggtaa attacggtat tgttggagct ggatattttg gagctgattt agctcgctca
     2701 atgaacaaaa ttgaagatgc aaaagtggtt gcggtatttg acccaaatca tggagaagaa
     2761 gttgctcaag agttgggatc agatgtttgt gcaagtttag atgaacttgt agcacgtgaa
     2821 gatattgatt gtgtgatcgt agcttcacct agctaccttc accgtgaacc agttgtgaaa
     2881 gctgctcaac atggcaaaca cgtattttgt gaaaagccaa ttgcattgtc ttatgaagat
     2941 tgtaaagcca tggttgacgc atgtaaagaa aataatgtca tctttatggc tggtcacatc
     3001 atgaacttct ttaacggtgt acaccatgct aaagaattga ttactcaagg taaaatcggt
     3061 aaagttcttt attgccatgc tgctcgtaca ggttgggaag aacaacaacc aactgtatca
     3121 tggaagaaac ttcgttctca atctggagga catttgtacc accatattca tgaattagat
     3181 tgcattcagt ttatcatggg aggacttcct gaaaaagcga caatggtagg aggcaatgta
     3241 tatcataaag gtgaaaactt tggtgatgaa gatgatatgc tcattgtaaa cttagaatac
     3301 tctgatgatc gttatgctgt tttggaatat ggtaatgctt tccgttgggg tgaacactac
     3361 gtcttgattc aaggaactga aggagctatc aaacttgact tgttcaatac tggcggtact
     3421 cttcgtgtta aaggtgaagg agaatcacac ttcttagttc atgaaactca agaggaagat
     3481 gatgatcgta cagctatcta taccggtcgt ggtatggatg gagcaattgc gtacggtaaa
     3541 ccaggagtac gttgcccatt atggttgcaa acatgtattg ataaagaaat ggaatatcta
     3601 catgacatca ttaaaggtgg agaaattaca gaagaatttg aaaaacttct caatggtgta
     3661 gctgctttag aatcaatcgc taccgctgat gcatgtactt tatcagttaa agaagatcga
     3721 aaagtaagtc tttcagaaat cacaaatgct taacttttgt aaaacagaat agtaaattct
     3781 tgtcattata taatttctaa agttctgtga tacaactcat tgaataaaga aatagagatg
     3841 ggactgggat aatgcccagt cccatttttt atcaaaaagt aatgagatca aaaatgtggg
     3901 agtgttgaaa tgaagattat aggtatcgat attggcggaa caacaattaa ggcagattta
     3961 tacgatgagt ttggaacgag tttgaatcat ttcaaagaga tagaaacaat tattgactat
     4021 gatttgggaa cgaatcagat attaaatcag gtctgtgatt taattggtga gtatacttta
     4081 aatcattcaa ttgatggtgt tgggatttcc actgctggag ttgttaatgc taatactgga
     4141 gaaatcatct atgcaggcta tacaatacca gggtatatcg gagtaaactt tactgccgaa
     4201 atagaaaaac gttttgggtt gtatactttt gttgaaaatg atgttaattg tgctgcatta
     4261 ggtgaattgt ggaagggaca agccaaagat aagaaaaatg tagtaatggt tactattgga
     4321 acaggtatag gaggcagtat tattgtcaac ggacaaattg ttaacggatt taactatact
     4381 gctggtgaag taggttatat tcctgtaggt aattcggatt ggcaaagtaa agcctcaaca
     4441 accgcattga ttcatttata tcaaaaaaag agcttgaaaa ctaatcaaac tggacgtact
     4501 ttcttcactg atttaagatc tggagataaa gttgctgaag aaacttttga aatttttgta
     4561 gaaaatctaa caaaaggttt attaacgatt tcttatctac ttaatccaga aattctcata
     4621 ttaggaggtg ggattctgga tagtaaggat attttgttac ctgaaattca aagttcttta
     4681 gctaaaaatg caatggataa taggttttta cctaaaaatc ttgtggcagc tacattagga
     4741 aatgaagctg gtcgtatagg agctgtaaaa aatttcttag atagaatttc taataaatag
     4801 tatgtaagat aaggaggtgt cacaatgact aactctgtat tttcgacaat gcaagatatt
     4861 gagaatgttg caaccgatat tataaaatca tatgataatg agatttatac ttataaagct
     4921 gtttcccaag aagaattgga aaaactagaa aaaagttatg atgaaaaaag tcacgaagaa
     4981 ttagtttcaa tagaaagcaa tttagaaatg aaacaacaga accttattga tgaggttaat
     5041 aaaacaatca aggaaaatga tgcaaatatt cagtatattt catcaagtag gagaggagaa
     5101 tttgtagaaa aaattattgg tagggtggta gaaaaatatg gccattagtc agatgaaaag
     5161 aatctctcta ctattttcta aaagtagtct tgatgatgtt ttaaaaacta ttcaagaact
     5221 agagtcagtg cagttccgtg atttaaaggt tcaggataac tggtcagaag ctctagaaaa
     5281 agatgaagtt gtatttccaa ctattcaaat ttttcatact tctaattcca atcatggggt
     5341 tattgaggga aatgatgcct tgacttattt gatgaatcaa caacaacatt tagaagcaac
     5401 tgtagagaaa ttacaagaat acctaccgaa agaaaacacg tttaaattat tgcagcaacc
     5461 tccgataact acctcttatg aagaattaga gaaatttggt aaagctaatg ttgctgaggg
     5521 tgttcttaaa aaagtgaatc atcaaattaa cagagttcat gaattagaaa gacacattca
     5581 aagtaataat gaggaaatag agcgattaat aaagtgggaa aaattagaaa ttgttcctgc
     5641 gaatttagaa caattttctt tctgtaaagg aaaagtcgga acaattccaa ggactgaaga
     5701 taatcgctta tacaatagtc ttttagaaaa caatattgaa gttcaagaaa tattttctaa
     5761 tgatagagag tacggtgttg ttgttttcta tcagtctagt tactctatag attttgatga
     5821 atacttattt gaaccatttg attattctag aaaggaatta ccgaagcagc gagtagtaga
     5881 tttagatcaa gaaaacatgc agttaataac tgaaaaagag aatattatcg catcgttgca
     5941 agattcaaag aaatatttga tagatttaca atggcaaata gactatattt tatctatcta
     6001 tgctcgtcaa atctctaaga ataacttttt gtgcactccg catctagttg cattagaagg
     6061 atggatagaa gaaactcgta ttttatattt tataaaagtt atggatgagc attt
//
LOCUS       DAVKJD010000086         5564 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKJD010000086 DAVKJD010000000
VERSION     DAVKJD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.086"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            966..2225
                     /locus_tag="VW157_001770"
     CDS             966..2225
                     /locus_tag="VW157_001770"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9553033.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADVRLVED"
     gene            2241..2792
                     /locus_tag="VW157_001771"
     CDS             2241..2792
                     /locus_tag="VW157_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9553034.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKTPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            2785..4062
                     /locus_tag="VW157_001772"
     CDS             2785..4062
                     /locus_tag="VW157_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9553035.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            4078..4938
                     /locus_tag="VW157_001773"
     CDS             4078..4938
                     /locus_tag="VW157_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9553036.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTSDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            5319..>5564
                     /locus_tag="VW157_001774"
     CDS             5319..>5564
                     /locus_tag="VW157_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEU9553037.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLHYVREYRTYEEIAADFGIHESNL"
BASE COUNT         1692 a          906 c         1262 g         1704 t
ORIGIN      
        1 tctagtttca atcaactata ttgttataaa ttgatttgaa tttcaaaatt aaattgtttg
       61 attcttattt caatttgtta tagtatatct gatgtcaaag ttctcggcga gtcaaatagc
      121 gattcccaag cctgactatc gtgaggtagt ggattaaaac ggtctgggga tagaccgttt
      181 taagtctgac gctggaaata agaatcgtca gaggaaggga tagcgaaatc gtggctttac
      241 gaacaggaac gtgatagtaa ggcgtatata gcggataagg gggtctcaaa ctctaaagtc
      301 caaaaaggta gtcgtaacct atatgcgtaa atcacgagag taattgaatt cgtactaaga
      361 ttttctattt tcactgtaac cttttaacgt ccttatatct tgtatacacg aggaaagatg
      421 tacgacttat cccgtgaggt ctatcactat aaagagaaaa cgacagatag aagtgatcct
      481 gagtcacggt tatctttaaa atagattgtc tgataggacg gtatgtataa aacgcttctg
      541 tgaactgaga gaaggggaga agttcttgct aaaatttagt tgaacagccg tattccgata
      601 cttagataag agatctagtc ttagatccta ctcagtttta ggggataaaa aaggggcaat
      661 agcgattcga gaaagattat actcttcgaa aatctcttca aatcacgtca atatcgcctt
      721 gccgtatatg taggatactg actacgtcag ttccatctac aacctcaaaa cagtgttttg
      781 agcaacctgc ggctagtttc ctagtttgat ctttgatttt cattgagtat tagtaattca
      841 gttactaact cgtcaactct gatttatcca ataaaattga aaaggatgga aaaaaggata
      901 aatttatgat atactttatt ttgaagacct tattagaaat cttgaaagag tattgaaaac
      961 ttagaatgag aaaaattgtt atcaatggtg gattaccact gcaaggtgaa atcactatta
     1021 gtggtgctaa aaatagtgtc gttgccttaa ttccagctat tatcttggct gatgatgtgg
     1081 tgactttgga ttgcgttcca gatatttcgg atgtagccag tcttgtcgaa atcatggaat
     1141 tgatgggagc tactgttaag cgttatgacg atgtattgga gattgaccca agaggtgttc
     1201 aaaatattcc aatgccttat ggtaaaatta acagtcttcg tgcatcttac tatttttatg
     1261 ggagcctctt aggccgtttt ggtgaagcga cagttggtct accgggagga tgtgatcttg
     1321 gtcctcgtcc gattgactta caccttaagg cgtttgaagc tatgggtgcc actgctagct
     1381 acgagggaga taacatgaag ttatctgcta aagatacagg acttcatggt gcaagtattt
     1441 acatggatac ggttagtgtg ggagcaacga ttaatacgat gattgctgcg gttaaagcaa
     1501 atggtcgtac tattattgaa aatgcagccc gtgaacctga gattattgat gtagctactc
     1561 tcttgaataa tatgggtgcc catatccgtg gggcaggaac taatatcatc attattgatg
     1621 gtgttgaaag attacatggg acacgtcatc aggtgattcc agaccgcatt gaagctggaa
     1681 catatatatc tttagctgct gcagttggta aaggaattcg tataaataat gttctttacg
     1741 aacacctgga agggtttatt gctaagttgg aagaaatggg agtgagaatg actgtatctg
     1801 aagacagcat ttttgtcgag gaacagtcta atttgaaagc aatcaatatt aagacagctc
     1861 cttacccagg ctttgcaact gatttgcaac aaccgcttac ccctctttta ctaagagcga
     1921 atggtcgtgg tacaattgtc gatacgattt acgaaaaacg tgtaaatcat gtttttgaac
     1981 tagcaaagat ggatgcggat atttcgacaa caaatggtca tattttgtac acgggtggac
     2041 gtgatttacg tggggccagt gttaaagcga ccgacttaag agctggggct gcactagtca
     2101 ttgctgggct tatggctgaa ggtaaaactg aaattaccaa tatcgagttt atcttacgtg
     2161 gttattctga tattatcgaa aaattacgta atttaggagc ggatgttaga cttgttgagg
     2221 attaaaccgt agaggtgttt atgaatattt ggaccaaatt agcaatgttt tctttttttg
     2281 aaacggatcg cttgtatttg cgtcctttct tttttagtga tagtcaggac ttccgcgaga
     2341 tagcttcaaa tccagaaaat cttcaattta ttttcccaac gcaggcaagt ctggaagaaa
     2401 gtcaatatgc actggccaat tactttatga agaccccttt gggagtgtgg gcaatttgtg
     2461 accagaaaaa tcaacaaatg attggttcta ttaaatttga gaagttagat gaaatcaaaa
     2521 aagaagctga gcttggctat tttttgagaa aagatgcttg gtcgcaagga tttatgacag
     2581 aggttgttag aaaaatttgt cagctttctt ttgaggaatt tggcttaaaa caattatcta
     2641 tcattaccca ccttgaaaat gaagctagcc aaagagttgc tcttaagtct ggatttagtt
     2701 tgttccgtca gtttaaggga agtgatcgtt acacaagaaa aatgcgggat tatcttgaat
     2761 ttcggtatgt aaaaggagag ttcaatgagt aagcatcagg aaattctaag ctatttggag
     2821 gaattgccag taggtaaaag ggtcagtgtt cgtagcattt cgaatcatct aggagttagt
     2881 gatggaacag cctatcgggc tattaaagaa gctgaaaacc gtggaattgt ggagacccgt
     2941 cctagaagtg gaacaattcg tgttaaatcc cagaaagttg ctatagagag attaacgttt
     3001 gctgaaattg cagaagtgac ttcttctgag gttttggctg ggcaagaagg tttagagaga
     3061 gagtttagta agttttcaat tggtgccatg actgaacaaa atatcttgtc ttaccttcat
     3121 gatggggggc tcttgattgt cggagaccga acccgtattc agttgctagc cttggaaaat
     3181 gaaaatgcag ttctggttac agggggattt caggttcatg atgatgtgct taaactggcc
     3241 aatcaaaaag ggattcctgt tctaagaagt aagcatgata cctttaccgt cgcgaccatg
     3301 atcaataaag ccttgtcaaa tgtccaaatc aagactgata ttctgacagt tgagaaactt
     3361 tatcgcccta gtcatgagta tggttttctg agagagacag atacagttaa agattatttg
     3421 gacttggttc gtaagaatcg tagcagccgt ttccctgtta tcaatcaaca tcaggtcgtt
     3481 gttggcgttg taaccatgag agacgctggt gataaatcac caagcacgac aattgataag
     3541 gttatgtctc gtagtctatt tttggttggg ttatcgacaa atattgccaa tgtgagtcaa
     3601 cggatgatcg cagaagactt tgaaatggta ccagttgttc gaagcaatca aactttgctt
     3661 ggcgttgtga cgcgacgaga tgtcatggag aagatgagcc gttcccaagt ttcggctcta
     3721 ccaacttttt ctgagcagat tggacaaaag ctctcttatc accatgatga agtagtcatt
     3781 acagtggaac cctttatgct agaaaaaaat ggagttttgg ctaatggtgt attggcagaa
     3841 attctgaccc acatgaccca agatttagtt gttaatagtg gtcgcaatct cattatcgag
     3901 cagatgctga tctacttttt gcaggctgtt cagatagatg atatattgcg cattcaggca
     3961 cggattattc atcatacgag acggtcagct ataattgatt acgatattta tcatggtcac
     4021 cagattgttt caaaagcaaa tgtgactgtt aaaattaatt agaaactagg agaaaagatg
     4081 ataacattaa aatcagctcg tgaaatcgaa gctatggaca aggctggtga ttttctagca
     4141 agtattcata taggcttacg tgatttgatt aagccaggcg tagatatgtg ggaagttgaa
     4201 gaatatgtcc gccgtcgttg taaagaagaa aatttccttc cacttcagat tggggttgac
     4261 ggtgccatga tggactatcc ttatgctacc tgttgctctc ttaacgatga agtggctcac
     4321 gctttccctc gtcattatat cttgaaagat ggtgatttgc tcaaagttga tatggttttg
     4381 ggaggtccca ttgctaaatc tgacctgaat gtctcaaaat taaacttcaa caatgttgaa
     4441 caaatgaaaa aatacactca gagctattct ggtggtttag cagactcatg ttgggcttat
     4501 gctgttggta caccgtccga agaagtcaaa aacttgatgg atgtaaccaa agaagctatg
     4561 tacaagggta ttgagcaagc tgttgttgga aatcgtatcg gtgatatcgg tgcggctatt
     4621 caagaatacg ctgaaagtcg tggttacggt gtagtgcgtg atttggttgg tcatggtgtt
     4681 ggcccaacta tgcacgaaga accaatggtt cctaactatg gtattgcagg tcgtggactc
     4741 cgtcttcgtg aaggaatggt cttaaccatt gaaccaatga tcaatacaag cgattgggaa
     4801 attgatacag atatgaaaac tggttgggcg cataagacca ttgacggtgg attgtcatgt
     4861 cagtatgaac accaatttgt cattacgaaa gatggacctg ttatcttgac tagccaaggt
     4921 gaagaaggaa cttattaata aaaagtgaaa agactactgg aagtttattt tgataaaaaa
     4981 tccagtagat cttttcataa taaaacgcat tgtatcaagt gttaggagct gatatcatgc
     5041 gtttttctgc ttttaagatt ttttccaact ctgtttgtaa gcgcatcata acaaagggtc
     5101 taggattcag ggctctcctc ctatatacta ttagtaaagt aaaactaagg gaggatattt
     5161 tagtgtcgca gtctattgtt cctgtagaga ttccacaata ttgtcgtttt gattctaaaa
     5221 agagaaatgg aattctgttt aacgttcgta ttgccaatct taaatttacc ttttttagat
     5281 tagactccct gcgaaacaaa atatggtata gtagttctat gaatgatgaa gcaagtaaac
     5341 aactaactga tgcacgattt aagcgtcttg ttggtgttca gcgcacgact tttgaagaga
     5401 tattagctgt attaaaaaca gcttatcaac ttaaacacgc aaaaggtgga cgaaaaccta
     5461 aattaagcct agaagacctt cttatggcca ctcttcacta tgtgcgagaa tatcgaactt
     5521 atgaagaaat tgcggctgat tttggtatcc acgaaagcaa ctta
//
LOCUS       DAVKJD010000087         5400 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKJD010000087 DAVKJD010000000
VERSION     DAVKJD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.087"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            103..423
                     /locus_tag="VW157_001775"
     CDS             103..423
                     /locus_tag="VW157_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9553038.1"
                     /translation="MQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICRDNAKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(926..1306)
                     /locus_tag="VW157_001776"
     CDS             complement(926..1306)
                     /locus_tag="VW157_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HEU9553039.1"
                     /translation="MELVLPNNYVDLEQEEMMYLDGGGVGRNWWNSRGSFATVLDVGL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALNVALTVTG
                     FSLGGAIAYGADWADGSLDGYIFA"
     gene            complement(1452..2804)
                     /locus_tag="VW157_001777"
     CDS             complement(1452..2804)
                     /locus_tag="VW157_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU9553040.1"
                     /translation="MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAY
                     IPKNYIVKANGNSVITGTEFLSAISSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQA
                     SSLNKQLVKLRAKEAIFQKFEQSLNEKYNRMSNSGEEQEYYGKVEYYLSQLNSENYNN
                     GTQYSKIQDEYTKLNKITAERNQLDADLQTLQNELIQLQQQGDSPSLSDTTSADDKAK
                     LETKILEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNDPTSQAYNIYAQLVSEL
                     GTARSNNNKSITELEANLGVATGQDKAHSILAPNEGTLHYLVPLKQGMSIQQGQTIAE
                     VSGKEKGYYVEAFVLASDISRVSKGAKVDVAITGVNSQKYGTLKGQVRQIDSGTISQE
                     TKEGNISLYKVMIELETLTLKHGSETVVLQKDMPVEVRIVYDKETYLDWILEMLSFKQ
                     "
     gene            complement(2801..3268)
                     /locus_tag="VW157_001778"
     CDS             complement(2801..3268)
                     /locus_tag="VW157_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEU9553041.1"
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLL
                     KAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVR
                     FEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFIPIVSL"
     gene            complement(3261..>5400)
                     /locus_tag="VW157_001779"
     CDS             complement(3261..>5400)
                     /locus_tag="VW157_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEU9553042.1"
                     /translation="YKKELSIMKIREIIGTDMYGTTVSGIVSGLNKLNFTVKAVRVAL
                     EDLTPKLTFPAILQVKNDLGQNHFVVLHSIKRNSKFYVADPASGIRKMSSDELGEIYQ
                     GITLFMVPNSDFERGKLKGKGLLDLFGRLIFNQKGLISTVILASFVLSIIGILSSLFS
                     KVIMDEVIPYALKNSLYMFLIVFGIVSFLQTLLSAFRQHVLLFLSRKIDIPVLMGYYD
                     HIIHLPYSFFGSRRVGDVLTRFQDAMTIKNVFTSVSISLVMDITLSVISAVVLWTINQ
                     SLFLILVFMVIVNIILIYCFKKPYKKINHEQMEANGLLNSQLIESIRNIDTIKSQHDE
                     EQRLNKIEEKFVHTLEIGYKEGVLQNIQSTISSMTSTMGGLLFMGVGALFIIDGKMTI
                     GDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQVAVSRLQELMEVDREDIALDYSIRDFT
                     LCDDIEFKDVTFAYGSRPPVIKDFNLRIKQGEKIAFVGESGAGKSTLVRLLLRFINPS
                     EGKIRIGENDLSDLDYGKLRKKISYIPQTIELFTGTIIDNLKIGNPSVTYEDMVRVCR
                     IVGIHDTIQRLQNRYGSFVEEDGQNFSGGEKQRLAIARALLSKADLYIFDEATSNLDS
                     FSEQIIQDLIFNKIMDKTTIVVAHRLSTILRCDKICFLENGTIVEYGTHEELMAKNGK
                     YARMVGLQSVQVNQQIQSQAVLDTEEVTYG"
BASE COUNT         1774 a         1070 c          755 g         1801 t
ORIGIN      
        1 tttataaaaa aattacaagc caaatttgta aagtttacac taagccgcta gggcaatcgt
       61 ctatcagaat gtccgtttat ttgtcaataa tccgagaaaa tcttgcaacg cttagaagtc
      121 tataaaaact atcaacattt atatgacttg cgaatgacaa ttctattaaa cctttccaca
      181 ctctatctat acaatcaaga taaaaacatg tgtaagcaaa tctgctacac tttactggag
      241 gacgccaaga ataagaaaag ctacgatagg ctggctatct gctatgtccg cattgggatt
      301 tgtagggata atgcgaaact tatccaaaaa gggttctcac tcctagagct gaccgaggaa
      361 acttctatgc tgtctcatct caaaaaagaa gtagagatct attaccaagc gaaggaaaga
      421 taaaaaagcc gagggatttc ctcgactctt tacatattat ttgttcgtta gttcttaata
      481 ccagccattg ttaagccaaa agtttttagc tctgccaagc cacatagtgt gtttactact
      541 atgtgtccat gtcaccatga atcgccgaac gatttaacga gaaagtatga cttttacgtt
      601 tatcccaact caattatgac atttttttca aaagtcaata tatctcactt tttcaacgac
      661 aagaaagagg ctgataatct accaacctct tgtttttaaa tacatttcaa gtccaaacta
      721 ttctttatag aaaaaagtcc tataaattcg agcaatagtt aaaatacaaa caaatataat
      781 tcccaaaata gataacacta ttttcacaaa attcatttca gaacctatta tatatacgtg
      841 aaaaaaaggg gctattccta cacatatata caataaataa cttacaaagt ttatttcacc
      901 aattcctgca aaaaaatgtt tcattttaag caaaaatata accatctaag ctaccgtcag
      961 cccaatcagc cccatatgcg attgctccac ctagtgaaaa tccagtaaca gttagtgcaa
     1021 cgtttagtgc agtattgact aaatggccag ctgcactacc tacatgttta attattaaac
     1081 tacgtaatgt tctcgtaata gcccctctat tagctgagat agcttttttt atcgcataag
     1141 cagaataaat tgttgcacca ccactataga tggccaaacc tacatccaga actgttgcaa
     1201 aactacctct actattccac cagttacgac caacaccacc cccatcaaga tacatcatct
     1261 cttcttgctc aagatcaaca taattatttg gtaatacgag ttccattaaa attctccttt
     1321 tttattaagc atggttagga tagtttccta acaagtgatc gttgactttg tttatagcac
     1381 ttagttacct agataagata tctttccttt atcttacttt attttatagg tgacttaatg
     1441 tttaaaacca attattgctt gaaacttaac atttccaaaa tccaatcaag ataggtttct
     1501 ttatcataga caatccgcac ttcaactggc atatcctttt ggaggacgac tgtctcactt
     1561 ccatgtttta gagttaaggt ttctaattct atcatgactt tatagaggct aatattcccc
     1621 tctttcgttt cttgggaaat tgttcctgaa tcaatctgtc tgacttgtcc ctttagtgtt
     1681 ccatattttt gactatttac accagtaata gcaacatcaa cttttgctcc ttttgagaca
     1741 cgagaaatat cactcgcaag tacaaaagct tctacatagt aacctttttc tttccctgaa
     1801 acttctgcta tcgtttgccc ctgctgaata gacattcctt gtttcaaagg taccaaataa
     1861 tgcagagtac cttcatttgg cgctaatata ctatgagctt tatcttgacc tgttgccact
     1921 ccaagattag cctcaagctc tgtaatactt ttattgttgt ttgaacgagc agtacctaac
     1981 tcactaacta attgagcata aatattataa gcctgagaag ttggatcatt ataggtacgg
     2041 ttcatatctt taatattgct ttgttgacta tcaatctcgc tattcttaga agtaatattt
     2101 gtttttaatg cttcgatttt tgttgttatt tctaatatct tagtttctaa cttagcttta
     2161 tcatcagctg atgtcgtatc tgataaggaa ggggagtctc cttgctgttg aagttgaatc
     2221 aattcatttt gtagagtttg caagtcggca tctaactgat ttctttcagc tgttatttta
     2281 ttcaacttcg tatattcatc ctgaatcttt gaatactggg taccattatt ataattttcc
     2341 gaatttaact gagataggta gtattcaact ttcccataat attcctgttc ttcaccagaa
     2401 ttagacatac ggttgtattt ctcatttaat gattgttcaa atttttgaaa gatagcttct
     2461 tttgcacgta acttcaccaa ttgtttattt aaagagctcg cttgtaaacc ttcttgtcca
     2521 cttgataacg aaataatgac atctccagca tttaccatat ctccttctga cttatgtaaa
     2581 gtaactacct tccctgaact aattgctgat aggaactctg tacctgttat gactgaattt
     2641 ccattcgctt ttacaatata gtttttgggt atataagctg cgccaaccaa tgcaccgctt
     2701 aagataatag cagttgaaat aatgagaata aacgcaaaag ctggtggtct cttatcaaag
     2761 aaaatgcgag aataacgtaa ttctgattta ttatataatt tcataggctt acaattggta
     2821 taaaaatatc tactaccatt ttttcaggag aagaattaac ataaactgta tagacaatcc
     2881 catccgtttg aatatcattt tcatagacat atagatccaa tttagaatac gcatactgta
     2941 gatactctgg actgtcttca aaacgaacat ataaacaata tggaacagag atagaatcct
     3001 gtacatcata aatgttactg tactgttgag cattatgagc ttgaatataa aactcaaaat
     3061 cagtcgttat taatccatca tcatgaatag tagtaccaca actttttaca attaatggac
     3121 caaaaatttg tgcttttaac aactgcaaat gttgatgaaa tttattaatt tcctgatcta
     3181 catcttctac ttttgtatca tgtaactttt tacagataac tgactttagt accagttttt
     3241 tattatcttt tacctctaac ttagccataa gtaacctcct ctgtatctaa cacagcctgt
     3301 gactgaattt gttgattcac ttgaacgctc tgcaaaccaa ccattctagc atactttcca
     3361 tttttcgcca ttagttcttc atgagtccca tattccacaa tagtcccatt ttctaaaaag
     3421 catatcttat cacatcgaag aattgtagac agcctgtggg ctactacaat tgttgtctta
     3481 tccattattt tattaaagat taaatcctga ataatctgtt cactaaatga atctaagtta
     3541 gaggttgcct catcaaatat atacaaatca gctttactca gtagtgctct tgcaatagcc
     3601 aatcgttgtt tctctccacc cgagaaattt tgaccgtctt cttctacaaa ggagccataa
     3661 cgattttgaa gacgttgaat cgtatcatga ataccaacaa tacgacaaac tctcaccata
     3721 tcctcatatg taacagaagg attaccaatt tttagattat caataattgt ccctgtaaag
     3781 agttctatgg tctgaggaat gtaggaaatt ttcttacgta gtttaccata gtcaagatct
     3841 gataagtcat tttctcctat acgaattttc ccctcactcg gatttataaa tcttaataac
     3901 aaacgaacta gcgtactttt tcctgctccg ctttctccaa caaaagctat tttttctcct
     3961 tgtttaattc gcaaattaaa gtccttgata acaggagggc gtgaaccata ggcaaatgtt
     4021 acatctttaa attcaatgtc atcacataag gtgaagtctc taatactata atccaaggca
     4081 atgtcttcac gatctacttc catcaattct tgaagccgac taaccgcaac ctgtacctct
     4141 tgaaaggtta actgcaagcc aactaaattc tgaataggtt ctgtaaaata ttgacttaat
     4201 gtctgaaaaa ccaagaggtc cccaatagtc atttttccat caatgataaa taaggcgccc
     4261 actcccataa ataataaccc acccatggtt gaggtcattg aagatattgt agattgaata
     4321 ttctgcaaaa caccctcttt ataaccaatt tctaaagtat gaacaaattt ttcttcaatt
     4381 ttgtttaaac gttgttcctc atcatgctga gacttaatgg tatcaatgtt acgaatagat
     4441 tcaattaact gtgaattaag aagtccattt gcttccattt gttcatgatt aattttttta
     4501 taaggctttt taaaacagta tataagaatg atgttaacaa taaccataaa aactaatatc
     4561 agaaataaag attgattaat agtccaaagg acaaccgcac taataactga caaggtgata
     4621 tccatgacta atgaaataga gactgacgta aacacattct ttatcgtcat cgcatcttga
     4681 aatcttgtta aaacatcacc aactctacga cttccaaaaa aactataagg aagatgaata
     4741 atatggtcat aatatcccat taaaaccggt atatcaattt ttcgagatag aaacaaaaga
     4801 acgtgctgac gaaatgcaga tagaagagtc tgtaagaacg acactattcc aaatactatc
     4861 aaaaacatat acagactatt ttttaaagca tatggaatca cctcgtccat gataactttt
     4921 gaaaagagac tcgataaaat accaatgata cttaaaacaa aggaagctaa tatgactgta
     4981 ctaatcaatc ctttttgatt aaaaattaac cttccaaata aatcaagaag tccttttccc
     5041 tttagtttcc ctctttcaaa atccgaattt ggaaccataa aaagagtaat accttgataa
     5101 atttctccca gttcgtcact tgacatcttg cgtatgccac tagcagggtc tgctacgtag
     5161 aacttactat tccttttgat actatgtaat accacaaaat gattttgacc taaatcattc
     5221 ttaacttgaa gaatcgcagg aaatgttaat tttggagtca aatcttccag tgctactcga
     5281 acagctttta ctgtaaaatt caacttattc agacctgaaa caataccact gacagtcgtt
     5341 ccatacatgt ctgtaccaat aatttcccta atcttcatga ttgatagttc ctttttgtaa
//
LOCUS       DAVKJD010000088         5220 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKJD010000088 DAVKJD010000000
VERSION     DAVKJD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.088"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(366..1094)
                     /locus_tag="VW157_001780"
     CDS             complement(366..1094)
                     /locus_tag="VW157_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9553043.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1252..2661
                     /locus_tag="VW157_001781"
     CDS             1252..2661
                     /locus_tag="VW157_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9553044.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWLERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2679..3974
                     /locus_tag="VW157_001782"
     CDS             2679..3974
                     /locus_tag="VW157_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9553045.1"
                     /translation="MKNSKFIDQFATFASKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3979..4287
                     /locus_tag="VW157_001783"
     CDS             3979..4287
                     /locus_tag="VW157_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9553046.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4284..4592
                     /locus_tag="VW157_001784"
     CDS             4284..4592
                     /locus_tag="VW157_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9553047.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIELYQNK
                     "
BASE COUNT         1760 a          881 c          948 g         1631 t
ORIGIN      
        1 ataaattggg agattgaaat tcatttctag caatttttta gaagtcacag tctactattc
       61 taacctcagt atactatatg tgttacaaga aaagactcaa aaacttctcg tcagtgtaag
      121 aatgtttgaa acactattta aaactaaata actataagaa gtagatcagg tgaacaattt
      181 cagtacctac tctataagct atacgaagat atttttcaac atgatggata aaacgatcac
      241 tcttttaaag ccatcttatt tagaaaagag aaaccattta agcatagatg ggaccttctt
      301 gccctgtttt aaatttttct taaactagag tctgtaaaag attggcttca ttttcacatt
      361 ttctcctagg aggaatgtcg taatgcaaaa gaattaaatt caaataaatc atagcgatga
      421 cgactaatcg agtactcaaa cggagttccg ttatccaaat aagccacttg ttctacttca
      481 aataccggtt ccgttggaag gagctgcaag taatgttgct cattttctga actagaagaa
      541 gctcgcaaga tttttgtagc actggcaata tgcaatccta actttccttc aatgtaattg
      601 taaatcgatt tttgtaaaat atcttcagta atacctggaa taacatcggt actcatataa
      661 gtttgttcca gaacataagg tttaccgtca ataatacgca gacggtaaat attataaact
      721 ggactctgca aagcgacctg tagtttttct gctaaaaatt catttgcaaa ttctaatttg
      781 aagtgaataa tcctcgagtc aattgtagaa tgagcatctt cagatatttt tgtcagtccc
      841 cggatatctc tttctggaac gattaatttt ctttttgagc tgccacgaga gagaacaaag
      901 gttccctgcc cacgttttct aaaaattaag ccctcagaaa ctaacaagtc taaagctttt
      961 ttgatcgtca ttcgactaca ggcaaactgt ttagctaatg tcatttcatc aggcaattgt
     1021 tcgttcatgg tatatttatt acaaagaatc ttttcacgaa tctcattgta aataaataaa
     1081 tattttgtac tcatttaatc tggttctctc tataatattc taatagtagt ataacaaagt
     1141 tcaagaaatg aggcaaacac aagatatgta tcaacaattt ttaaaaatgt ctagacaaat
     1201 tttaaaatta tgctatactt tatgtataga ataatagaaa ggaggttgag catgaaaaaa
     1261 aagttagtat ttcctaatct gttttggtgg ggagctgctt ctagcggacc tcagacagaa
     1321 ggtcaatatg gaaaagtaca tgaaaatgtg atggactact ggttcaaaac gcatccagaa
     1381 gattttttcg ataatgtcgg acctcttgta gccagtaact tttttcatac ttacaccgaa
     1441 gatttccact tgatgaagga aattggagtt aattctttcc gcacttccat ccaatggagt
     1501 cgactcatca agaatttaga gacaggtgag cctgatccaa aaggtattgc tttctacaat
     1561 gccatcattg aagaagctaa aaagaaccag atggatcttg tgatgaattt acatcatttt
     1621 gatttaccag tggaacttct tcaaaaatac ggtggttggg aaagcaaaca tgtagtggag
     1681 ttattcgtga agtttgccaa gactgctttc acatgctttg gagataaggt tcattactgg
     1741 acaactttca atgagccaat ggtcattcca gaagcaggat acttatatgc tttccattat
     1801 ccaaatctaa aaggaaaggg aaaagaggcc gtacaagtca tctataatct aaaccttgct
     1861 agtgcaaaag tgattcaact atatcgctca ttagaacttg atggaaagat tgggattatt
     1921 ttaaacttga cacctgctta tccaagaagt aattctccag aagacttaga agcaagtcga
     1981 tttacagatg acttctttaa caaagtcttc ttgaatccag ctgttaaagg aactttccca
     2041 gaaagattgg taaaacagct agagagagat ggcgtgttat ggagtcatac cgaaaaagag
     2101 cttcaactga tgaaatcaaa tacggttgat tttcttggag taaactacta ccatccaaaa
     2161 cgtgttcaag cacaagcaaa tcctgaggaa tatcagacgc cctggatgcc agaccaatac
     2221 ttcaaagagt atgaatggct ggagcgtcgc atgaatccat atcgtggttg ggaaattttt
     2281 ccgaaagcca tttatgatat tgctatgatt gtgaaggaag aatatggtaa tatcccatgg
     2341 tttatcagtg aaaacggaat gggtgttgaa aacgaagcac ggtttatcga tgaaaatgga
     2401 gttatcgatg acgtgtatcg tattgaattt tatgaagaac atttaagatg gctacataaa
     2461 gccattgaag agggaagtca ctgttttgga taccacgctt ggaccgcatt tgattgctgg
     2521 tcttggaata atgcatataa gaatcgttac ggatttatct ccgttgattt agaaacgcaa
     2581 aagagaacca tcaagagctc aggaagatgg tatcgcaaag taagtgacaa taacggtttt
     2641 gaagtagaaa ttgaggagta aagagaggag atattcatat gaaaaactca aaatttatag
     2701 accaatttgc cacctttgct agtaaactag ggaaccaaat tcatttaaaa accctaagag
     2761 atgcattcgt aacagtaatg ccattatata ttttggcagg tttaatcgtt cttttgaaca
     2821 acacggtatt taagtggatt ttccaagggg atacattaac aagattccaa tattggggaa
     2881 taacaattgc aaacggtact ttaagtattt caggtatgat tattgctgta atggttggtt
     2941 atttcttagc taaaaacaga gatttcgaaa acccgttagc agcatcaatg ctatcattag
     3001 tttctttaat tgtgatgatg ccaaatacag tttctgtagt tcctgacgga gcaaaagatg
     3061 cggtaaacat ttcaggtgtt ctttcattca acaacacagg tacaggcgca atgttcgccg
     3121 gggttatcgt agcgattatt gcaacagaat tattcattga attatcaaac gttaaagctt
     3181 tacaaatgaa ccttggtgaa aatattccac cagctgttag tagatcattt agcgtattac
     3241 ttccagtcat gaccgtcatc tccttatttg gggttgtttc agcattatta ttcaatataa
     3301 ctggaatgaa cttaatctca atcattacaa tctttattca agaaccaatt cgtcatattg
     3361 gtacaagctt aatcggggtc attattattt actctttagg aaatatgtta tggctatttg
     3421 gtattcacca agcagttatt tacagtgcca tcctagaacc attactatta attaacatta
     3481 ctgaaaacat cactgcagca aataatggac aagccattcc acacatcatc aacctatcac
     3541 aaatacaaac attcgcttta atgggtggta gtggatctac attatgttta ttaatagcaa
     3601 cattcttagt gagtcgcaat gctgtctcta aaaacgtggc taaattatct tttggacctg
     3661 gtatcttcaa tatcaatgaa ccagtattat tcggttaccc aatcgtttat aacatttcat
     3721 tagctattcc atttatcaca gttccagtcc ttggtatttt aatcagctac ttagcaacag
     3781 ttacaggatt catgagtcct gcatttatac aagttccttg gactacacca gtattcttaa
     3841 atgcatggtt agcaacagca ggggacgtga gagcagttct agttcaattc atcatctttg
     3901 cacttggagt ccttctatac attccattta tcaaagttaa tgacaaagtt gttgaacaag
     3961 aaatggaagg ttaagattat gaaacaaatt ttattagtat gtaatgcggg aatgtcgaca
     4021 agtatgcttg ttaaaaaaat gcaacaaagt gcgactgaac gtgggattga aatctctatc
     4081 caagcaaaat caatgacaga agctaaaaag aatatccatg aagcagatgt cattctaatt
     4141 gggccacaaa ttcgttatga gttacttgct gtgaaagaaa ttgcaggaaa tatccctgta
     4201 gataccattg atatgcggga ttacggaatg atgaatggag caaaagttct ggaacaagca
     4261 cttgcatgga taggagaaat aagatgacag aagaaatgga atatatctgt tttcagctca
     4321 ttgccaatag tggagcagcc aaatcatcct ttattgaagc catccaacta gctaaggcag
     4381 gaaatttaaa agaagcgaaa attaaagttg aagaagcgga agactcctta gtagaagcgc
     4441 acaagattca ctctaatctc atacaaaaag aagctactgg agaaaaaata ggattctcct
     4501 tgctatttat gcatgcagaa gaccaaatgg cttcaacaga gattattcag ttgctatcta
     4561 aagaattcat cgaactctat caaaataaat aggaacactg tcattcccat ctgtctttat
     4621 taatgacgac tacattgtag gaacctccaa aagttggaca aaatgattga aaggattgga
     4681 tcctgcatta cacaagatcc aatccttttc ttttactcta gtttttataa tatgttgaat
     4741 ctagaatagt ccactgtagt ttctaaaaaa ttgctggaaa tggatgttaa gctccaattt
     4801 atttgtttat atcttatttc agtttactat actagcttat tattctagtg aaaaattgaa
     4861 tgagccacta tttatttaat atgtatgatg agggaatgac tcaaaaactt cttgtcgata
     4921 taagaatatc tgagatagta ttttaaaacg aaaggactat aaaagctcat ttccttcgca
     4981 cttattttat agagatctat cattatactc tttagaaatc aaaaagcaac acaccttgtc
     5041 atcgttttat cgtaaatctg ttaatgaggg ggctagtctt atctgcaatc tcaaatcagt
     5101 gttttgagct gaatggcact cgttttctag tttgctcttt gattttaatt gggccttagt
     5161 aaatactact cttaaattaa actatagtga attgaaataa gatgtgaaca actctatcag
//
LOCUS       DAVKJD010000089         5218 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKJD010000089 DAVKJD010000000
VERSION     DAVKJD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.089"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..553)
                     /locus_tag="VW157_001785"
     CDS             complement(<1..553)
                     /locus_tag="VW157_001785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553048.1"
                     /translation="MSRVKNSFFNILAGIVGTIISSVLAFIVRTVFIRVLGETYLGFN
                     GLYTNILTVLSLAELGIGSSIAYLMYKPLAEKDGDKLAQVVQFYKKIYRVIGIIILIL
                     GLCLVPFLPVIVNLKNAEDLNYTALYLLYLADTVSTYLFFAYKRGVLVADQKVYVANI
                     FDIIITTILSIFQIITLMIFKDFY"
     gene            complement(550..1737)
                     /locus_tag="VW157_001786"
     CDS             complement(550..1737)
                     /locus_tag="VW157_001786"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9553049.1"
                     /translation="MKICIVSVGVGGLPIPAVKGGAVENLIDNYLSYNEKYNHDEITV
                     VSCDHKEAREASKKYKYAQFVYIDIHSLKYKINKTIRYAINKYSPFFVGNAYISQLPD
                     LSGFDTVLIENRPEYGYYIRKKFKGNLVLHLHNDLLMDNEYSVDYSVYDKIITISDYI
                     RDRSEVVMSGVPIQTVYNGIDTELFLQNFSEIDLSDLKNQLEILSDDFVIVFFGRINK
                     NKGIKELLEAFLLLPKNLNIKLLAVGSSIFGQTELDTFTTELRQLAKQASDKVVFTGY
                     VNYKDIPKYHHIADCIVVPSIWEEPAGLTVCEALISGKYVITTDAGGIPEIVAGSEAL
                     VVANDECIVEHLKTALLSVYQKGKCSSVITSNRDRGAYFSIEKYGRDLRKELTQNERI
                     INI"
     gene            complement(1779..2957)
                     /locus_tag="VW157_001787"
     CDS             complement(1779..2957)
                     /locus_tag="VW157_001787"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9553050.1"
                     /translation="MKIVHVEDFFHPDAGYQINVLPKYLAKFGHEQVIITSEMDKIPD
                     NLTQFFGRENIEERDRSYEEKHHVKIIRLPLHGFVSGRAIFSGKLISAIKGLSPEVLY
                     IHGNDTLTGIRLLLARKKLNCRIVTDSHMLEMASRNPFNNYFRRFYKTLITPILIKEQ
                     IPIIRTQDDNYVEKHLGIPLSQAPWISYGSDTAFFHADSQIKEDFRSQYQIASDALIC
                     VYAGKLDEHKGGMFLAESLSKKLNTEQPIVFIIVGNTNGEYGEAVEKSFSQSENQILR
                     FPTQKYQKLAQFFQVADFALFPKQCSLSFYDAQACGLPVLLEDNNINLDRTSHYNGWT
                     FKANSKLSLRDSLKMIASLDKEKRQEYSQNALHYILEHYNYEDKAREYEKILIGERTK
                     "
     gene            complement(2959..4035)
                     /locus_tag="VW157_001788"
     CDS             complement(2959..4035)
                     /locus_tag="VW157_001788"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9553051.1"
                     /translation="MKLAILLSDISKIGGVQRVTTALVNELCQNIDVTIISIFSENEL
                     PAFGLSDRVQVSYLLKEHVNLKKNFFKVSGALRKVLKKQQFDVVLSSGTGLVSFLWFA
                     SLGIPTRLLAWEHQCFYFGRSFGLEWLGRKLACRFAESIVVLTKKDEGFYKENRPKAH
                     IEQIYNILDMDSQTAKCQLSSKKIISVGSLVRQKGFDLALEVASQIQLSYPDWQWDIY
                     GDGPDREKLEEKVREYQLEGFINLKGLVQNVRELYPDYSIYALTSRYEGLSMVLLEAK
                     NCQMPAISFDCNCGPSDIISDGRNGFLIDCFDVNIYAKTLSKLMTSLELREQVAKSSV
                     IPAEELSTTYILDKWNNLLKIERR"
     gene            complement(4010..>5218)
                     /locus_tag="VW157_001789"
     CDS             complement(4010..>5218)
                     /locus_tag="VW157_001789"
                     /inference="COORDINATES: protein motif:HMM:NF016800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9553052.1"
                     /translation="MKRIYYHLLAIWAWTLPNSYAFIDSLKVFFPNISLQIAGSLLAV
                     VSIGIFITRIYYTRYEVFISLLVCISILIFYSTRFFYSTNVELYQSFFNSFLIRPVPA
                     ILVAMLLAKNNHIKGFIKWSEPMMLFYTLTSFLAALSPRNSIITYQSLSYYAMTAYMI
                     NVFNIIYKEKILEEQLTYFRFSAWNFIRYFLLLIQAFNALSGGGRGAFILLIIFTGIL
                     LIRTAMKFKFLFSFIGGLSAIALVVFFTKGLDLTWLLNMDGGERLLNFFGRPEHISTD
                     NRLLIYDTVWSAIQEKPFLGWGVGSTFLKFNGYSHNIVLDLLHDMGSFGLLIILSAFF
                     ASSVILYKLRKIDWKVNLFILMFLEVYVHMSFSGSYLADGRLWFLVIFTYCYYRWKVQ
                     EKDEVSNFII"
BASE COUNT         1814 a         1016 c          738 g         1650 t
ORIGIN      
        1 gataaaaatc tttaaaaatc atcaaagtga taatttggaa aatagataaa attgtcgtta
       61 taattatatc aaagatattc gcaacataga ccttttgatc agcaaccaat acccctcttt
      121 tatatgcaaa gaatagataa gtagaaactg tatctgctaa ataaagcaaa tacaatgctg
      181 tatagttcaa atcttctgca ttttttaaat ttacaatgac tggtaaaaaa ggaacgagac
      241 acaaacctaa aataagaata attataccaa ttactcgata tattttttta tagaattgaa
      301 ctacttgagc taatttgtcc ccatcttttt ctgctaaagg tttgtacatc aaataagcaa
      361 ttgatgatcc gatccctaat tcagctaggg ataaaactgt taagatattg gtgtatagac
      421 cattgaaacc tagataagtc tctcccaaaa ctcggataaa cactgttcta actatgaaag
      481 ctaaaacact cgatatgata gtaccaacta ttccagctaa aatattaaaa aaactatttt
      541 ttacacgact catatgttaa tgattctttc attttgtgtg agctctttcc gtaagtctct
      601 tccatatttt tcaatagaaa aataggctcc tctatctcta ttagaagtaa taaccgaaga
      661 gcatttccct ttttggtaga cactcaatag ggctgttttg aggtgttcaa ctatacactc
      721 atcatttgct accacaaggg cttcactgcc agctacaatc tctggtatcc ctccagcatc
      781 tgttgtaata acatatttcc ctgatatcaa agcttcacag accgtcaaac ccgctggttc
      841 ctcccaaata gatggaacaa caatgcaatc tgcaatatga tgatacttag gaatgtcttt
      901 gtaattaaca tagcctgtaa aaacaacttt atctgatgct tgttttgcca attgccgtag
      961 ctctgttgtg aaagtatcca actccgtctg accaaatata gaactaccaa cagctagtaa
     1021 ttttatattc aaattctttg gtagcaacaa gaaggcttca agcagttctt tgataccttt
     1081 atttttgttg atacgtccaa aaaaaacaat cacaaaatca tccgacaaaa tttccaactg
     1141 atttttaaga tctgataaat caatttcaga gaaattctgc aaaaaaagtt ctgtgtcaat
     1201 accattatag acagtctgta tcggaacacc tgacattact acttctgacc tatccctaat
     1261 atagtcagat attgtgataa ttttatcata aacgctataa tctacagaat actcattgtc
     1321 cattaataaa tcattgtgaa gatgtaaaac aagatttcct ttaaactttt tacgaatata
     1381 ataaccgtat tcagggcgat tttcaatcaa aactgtatca aaaccagaca agtctggcaa
     1441 ttgactgata taagcgttcc ctacgaagaa aggtgaatat ttattaatgg cataacgtat
     1501 tgttttgttt attttatatt taagtgaatg aatatctata tagacaaatt gggcatactt
     1561 gtattttttt gaagcctcac gcgcctcctt atggtcacaa ctaacgaccg taatttcatc
     1621 atgattatat ttctcattgt aacttaaata attatcaatg agattttcta ctgctccccc
     1681 ttttacagct ggaatcggta gtccgcccac tccgactgaa acaatgcaaa ttttcattaa
     1741 atccaccttt cctataaaat aacgaaatat taactatctc atttcgtacg ttcccctata
     1801 agaatctttt catattctct tgctttatct tcataattat aatgttcaag gatatagtgc
     1861 agtgcattct gagagtactc ttgtctcttc tctttatcta gactcgcaat catttttaat
     1921 gaatctctca aacttaattt gctgttagcc ttgaacgtcc aaccattata atgagaagtt
     1981 ctatcgaggt tgatattatt atcttccaat aaaactggta aaccacaggc ctgtgcatca
     2041 taaaaactta aactacattg tttaggaaat aaagcaaaat ctgctacctg aaaaaactgt
     2101 gctaattttt gatatttttg agtcggaaag cgcaaaattt ggttttcaga ttgggagaaa
     2161 cttttctcga cagcttctcc atactctcca tttgtattac ccactataat gaaaacaatt
     2221 ggttgctccg tatttaactt tttacttaga gactctgcta aaaacatccc acccttatgc
     2281 tcatctaact ttcctgcgta aacacaaata agagcatctg aagcgatttg atactgagaa
     2341 cgaaaatctt ccttaatctg gctatctgcg tgaaagaacg ctgtatccga cccataagaa
     2401 atccatggag cttgcgataa aggaattccc aagtgttttt caacataatt atcatcttgc
     2461 gtccgaataa ttggtatctg ttcttttatc aaaatgggag taatcaaggt tttataaaat
     2521 ctcctaaagt agttgttgaa aggatttctt gatgccattt caagcatatg actatcggtg
     2581 acgattctgc aattaagttt ctttcgagct aaaaggagac gaatccctgt taaagtgtca
     2641 ttcccatgaa tatacaatac ctctggagaa agacccttaa tagccgaaat aagctttcca
     2701 gaaaaaatcg cacgtccgct tacaaatcca tgaagaggca agcgaataat cttcacatga
     2761 tgcttctctt cgtaacttct atctctctct tctatatttt ctcgtccaaa aaattgggta
     2821 agattatcgg ggattttatc catttctgaa gtgataatca cttgttcatg accaaacttt
     2881 gctagatatt ttggtaaaac attaatctgg taacctgcat ctggatgaaa aaaatcttct
     2941 acatggacta ttttcatatt atcttctttc tatctttaat agattgttcc acttatctag
     3001 gatataagtt gttgacaatt cttctgctgg aataacactc gattttgcaa cttgttctcg
     3061 caactctaaa ctagtcatta atttagataa agtttttgca tagatattta catcaaaaca
     3121 atcaatcaaa aaaccatttc ttccatctga aataatatca gagggtccac agttacaatc
     3181 aaatgaaata gcaggcatct ggcagttttt agcttctaat aataccattg ataatccttc
     3241 atatcgtgat gtcaaggcgt agatagagta gtcaggatag agctcccgaa cattctgaac
     3301 aagccctttc aaatttataa agccttctaa ttggtattcc ctgactttct cctctaattt
     3361 ttctctatcg gggccatcac cataaatgtc ccactgccaa tctggataac ttaattgtat
     3421 ttgactagct acctcaagag ccagatcaaa acctttttgt cgtactaaag atccaacact
     3481 gataattttt ttagaagaga gctgacactt agctgtttgt gaatccatgt ctaggatatt
     3541 atagatttgt tcaatatgag ccttaggacg attctctttg taaaatcctt catctttttt
     3601 agtcaagaca acaatactct cagcaaaacg acaggctagc tttcgcccca accactctag
     3661 accaaacgat ctcccaaagt aaaaacactg atgttcccat gccaaaagtc tggtaggaat
     3721 tcccaaacta gcaaaccaaa gaaaggatac tagaccagtt cctgacgata aaacaacatc
     3781 aaactgctgt tttttcaaca cttttcgcaa agctccacta actttaaaaa aattcttctt
     3841 caaattaaca tgttctttca aaagatagct cacctgaact ctatctgaaa gtccaaaagc
     3901 tggtagctca ttttccgaaa agatgctgat aattgtaacg tctatatttt gacaaagttc
     3961 attaacaaga gctgtcgtga cacgctgcac tcctcctatt tttgaaatat cagataataa
     4021 aattgctaac ttcatccttt tcctgcactt tccacctgta gtaacaataa gtaaagatca
     4081 ccaaaaacca tagtctacca tctgcaagat agcttccact gaaactcatg tgaacatata
     4141 cttctagaaa catcagaata aataaattca ctttccaatc tattttacgt aatttgtaca
     4201 atattactga agaagcaaag aaagccgaaa gaataattaa taaaccaaaa cttcccatat
     4261 catgcaataa atccaataca atattatgtg aatatccatt aaattttaaa aaagttgaac
     4321 caactcccca tcctaaaaag ggtttttcct gtattgcaga ccaaacagta tcataaatca
     4381 atagtctatt atctgtagat atatgttcag gtctgccgaa gaagttcaac aatcgctctc
     4441 caccgtccat attcaagagc catgtcaaat ccaaaccttt tgtaaaaaaa acaactagag
     4501 ctatagcaga caggcctcca ataaatgaga aaaggaactt aaatttcata gcagttctta
     4561 ttaataatat tcctgtaaaa ataattaata aaataaaggc acctctccca cctcctgaga
     4621 gtgcattaaa agcttgaatc aaaagtaaaa aatatctaat aaaattccac gctgagaatc
     4681 tgaaatacgt cagttgctcc tccaatattt tctctttgta tataatatta aacacattaa
     4741 tcatataagc tgtcatggca taataagata aagattggta agtaataatg ctgtttctag
     4801 gagatagggc tgctaaaaat gatgtcaatg tatagaaaag catcataggt tctgaccatt
     4861 ttatgaaacc ttttatatga ttattcttag ctaacagcat cgctactaat atcgcaggaa
     4921 ctggacgaat cagaaaggaa ttgaagaaag actgatacaa ctctacgtta gtcgaataaa
     4981 aaaagcgagt tgaataaaaa attaaaatag aaatacaaac caatagagat ataaaaactt
     5041 catatcttgt ataatatatt ctagtgataa aaattcctat tgagacaaca gccaaaagag
     5101 aacctgcaat ttgtaagcta atattcggaa aaaacacctt taacgaatca ataaaagcat
     5161 aagaatttgg aagagtccaa gcccaaatag ccaaaaggtg ataataaatt cttttcat
//
LOCUS       DAVKJD010000090         5171 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKJD010000090 DAVKJD010000000
VERSION     DAVKJD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.090"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            77..1066
                     /gene="manX"
                     /locus_tag="VW157_001790"
     CDS             77..1066
                     /gene="manX"
                     /locus_tag="VW157_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9553053.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            1094..1897
                     /locus_tag="VW157_001791"
     CDS             1094..1897
                     /locus_tag="VW157_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9553054.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            1921..2832
                     /locus_tag="VW157_001792"
     CDS             1921..2832
                     /locus_tag="VW157_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9553055.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2888..4222)
                     /gene="pepC"
                     /locus_tag="VW157_001793"
     CDS             complement(2888..4222)
                     /gene="pepC"
                     /locus_tag="VW157_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9553056.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(4334..5056)
                     /locus_tag="VW157_001794"
     CDS             complement(4334..5056)
                     /locus_tag="VW157_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9553057.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILLASIA"
BASE COUNT         1407 a         1150 c         1062 g         1552 t
ORIGIN      
        1 tgaaaacggt ttatacttaa acagtcttaa agttttctta aatgaaaact aaaacaaaaa
       61 aggaggaatg acaataatga gtatcggaat cattattgcg agccacggcg aatttgctgc
      121 gggtattcat cagtcaggat ctatgatctt tggtgaacaa gaaaaggttc aagttgtgac
      181 ctttatgcca aatgaaggtc ctgatgatct atacgctaag tttaataacg ctgttgctgc
      241 atttgacgca gaagatgagg ttctagtttt ggctgacctt tggagtggtt ctccatttaa
      301 ccaagctagt cgcgtgatgg gagaaaatcc tgagcgtaag tttgccatca tcacaggact
      361 taacttaccg atgttgattc aagcctacac agagcgcctc atggacgctg ctgcaggtgt
      421 agaaaaagtc gctgctaata tcattaaaga agccaaagat ggcatcaaag ctcttccaga
      481 agagctaaat ccagttgaag aagttgcaag cgctgcagct gctccagttg cccaaactgc
      541 tatcccagaa ggaactgtta tcggagacgg taaattgaaa atcaatcttg cccgtcttga
      601 cacacgtcta cttcacggtc aggttgcaac tgcttggact ccagattcaa aagcaaatcg
      661 tatcatcgtt gcttcagata acgtggctaa agacgacctt cgtaaagaat tgattaaaca
      721 agcagctcca ggtaatgtca aggctaatgt ggttccaatt caaaaactga ttgagatttc
      781 aaaagaccca cgttttggag aaacacatgc ccttatcttg tttgaaacac ctcaagatgc
      841 ccttcgtgcc atcgaaggcg gcgtgccaat caagactctt aacgttggtt ctatggctca
      901 ctcaacaggt aaaacattgg tcaataccgt tttgtctatg gacaaagaag atgttgctac
      961 atttgaaaaa atgcgtgact tgggtgttga atttgatgtc cgtaaagtac caaatgattc
     1021 taaaaaagat ttgtttgact tgattaacaa agccaatgtc aaataagcca ttatttatga
     1081 aaggatttta aacatgtcta ttatttctat ggttttagta gtcgttgtag ccttctttgc
     1141 aggtcttgaa ggcatcctcg accagttcca atttcaccaa ccacttgtag cctgtaccct
     1201 tattgggctt gtaacaggtc acttggaagc agggattatc ctcggtggat cgcttcaaat
     1261 gattgccctt ggttggtcaa atatcggtgc tgctatcgct cctgatgctg cacttgcttc
     1321 tgtcgctgct gccattatca tggttcttgg tggtgacttt accaagactg gtatcggtgt
     1381 tgcccaagcg gttgctatcc ctcttgccgt agctggactt ttcttgacaa tgattgttcg
     1441 tacaatttca gttggtttgg ttcatactgc agatgctgcc gctaaaaaag gtgacttcgg
     1501 cgctgtggag cgtgcgcatt tcattgcgct acttttccaa ggacttcgta tcgcgcttcc
     1561 tgcagctctt ctccttatgg taccaactga aactgtacaa agtatcctta gtgccatgcc
     1621 agactggctc aaagatggta tggctatcgg tggtggtatg gtcgttgccg ttggttacgc
     1681 catggttatc aacatgatgg caactcgtga agtatggcca ttcttcgctc ttggtttcgt
     1741 tctcgctgct gtgtcagata ttactctaat cggattcggt gctatcggtg ttgctatcgc
     1801 tcttatctac cttcaccttt ctaaaactgg tggaaatggt ggcggaggag ccgcaacttc
     1861 taacgaccca atcggcgata tcctagaaga ctactaagat aagaaaggac tgaaaacatc
     1921 atgactgaaa aacttcaatt aactaaatca gatcgtaaaa aagtttggtg gcgttcaacc
     1981 ttcttacaag ggtcttggaa ctttgaacgg atgcaaaact tgggctgggc ttatacactc
     2041 attccagcta tcaaaaaact ctatactaaa aaagaagatc aaatcgctgc tcttgagcgt
     2101 caccttgagt tcttcaacac tcatccatac gtagctgctc cagtcatggg ggttactctt
     2161 gcgcttgaag aagaacgtgc taacggtgtg gaaatcgatg acgctgctat ccaaggggtt
     2221 aaaatcggta tgatgggacc tcttgctggt atcggtgacc cagtattctg gtttacagtg
     2281 cgcccaatcc ttggatctct cggtgcttca cttgccctta ctggcaatat cttgggtcca
     2341 ctcctcttct ttgttgcatg gaacttgatt cgtatgtcat tcttgtggta tgttcaagag
     2401 attggataca aggctggatc agaaatcact aaagatatgt ctggtggtat ccttcaagat
     2461 atcactaaag gagcttctat ccttgggatg ttcattcttg ctgtccttgt tcaacgctgg
     2521 gtaaatatta aatttgcttt cgatgtttct aaagttcaac tagatgaaaa ggcttatatc
     2581 cattgggata aattgccaga agggtctaaa ggtatccaag aagcattcgc acaagtagga
     2641 caaggattgt ctcaaactcc tgaaaaagtt actactttcc aacaaaactt ggatatgttg
     2701 attcctggat tatcaggact actccttact ttactttgca tgtacttact taagaaaaaa
     2761 gtatctccaa tcactattat ccttgccctc ttcgcagtgg gtattgtggc acatgttctt
     2821 cacatcatgt aatcaagcaa ctaaaaagga accaggttct aaaatctgat tccttttttc
     2881 tatgctttta ttcagccaag gctcccattg gatcccatgg tgcaagtacg attggttctg
     2941 ctccataggc agcttgttct tctgctgtca gcaattcctt acgaacaacg atttggtatg
     3001 tgtattcgtc catccaagcg tctgaggcaa caaagtaacc atctgtaccg accttgtctc
     3061 cccatgagtt ttcaaccttc cacttggttg atttaccatt ttcgtccaag tcaacacctg
     3121 tcaagaccat ggcgtgggtc atcaagcttt cgctatagtc caaacgtcca gccttgtctt
     3181 gagtaagttt aatgtccatg cttgattcaa agtcataaac atctgtcgca aggatgccag
     3241 ccttacggtt gctgagctgg ccgacatcag aaccaaacca aacagtctca cctgcttgca
     3301 tttgggcaat cgccaattct ttcaagcgct ccattggaac gttgatgtag cgaactgcac
     3361 ggctaccaac cacattcccc aacatctcaa ctgtgtaaga ttttccgtaa ggtttatcag
     3421 cagttggagc attgataaca gaaacgtagt cttctaaagg aagattgaca tatttcttgt
     3481 aaaactcttg tggtgtgatt cctttttcac ttttatagtt gttatcttta tcgcgataag
     3541 caaagtcaaa cttgcgtggt ggaagtccta atgacatagc aagaaagtta aagatttctt
     3601 gcaagaggtc ttctttctta gcttgaacag tcgcttgatc tgcaccagaa acaagcaaat
     3661 cacgcaagat ttgagcatct tgacgaagca atttattaag gatcgcattt agctcacgac
     3721 tgctgctaga tgaaacagac tcaggataaa ctgacttagg cacgacaccg tatttttcaa
     3781 agagggaaac gaccatatcc cattgaccgc catcttgttg aggtgtttgg agtaagaagc
     3841 taaccttgcg gctagtcaat tcttggtctg aagtcgcaat gacttgctcc aagaaccagt
     3901 ttgatttctc atacttatcc cagaagaaag tgtgggcttg tgacaactca aagttctcca
     3961 atttgtattg cgagatgagt ttgtggcgga aggtgttgag agccgcaaac atccagcaac
     4021 gaccagacgc tttctggtta gtgaccttgt ccttggttaa atccaatgag aaaacaggtg
     4081 tgttgtctac atggctttgg cgacattcca gagctgcaaa aattccgttg tggctggcag
     4141 cattttcaat cgcttggtat tttacatttg cttcatagtt ggcaaatagt ttatcagtaa
     4201 atgattcttg aatcgcgttc atagattcct ccttttattc tacagtctat tataactctt
     4261 ttcacaaagg aagcaactaa aaaacataaa aggtaaggac attctccctc acctttttcg
     4321 aatataaatc aaattaagca atactagcaa ggagaatttc taattcttcc ttggtcaagc
     4381 gacgccattc ccctcgttcc aggttctcat ctaatactaa agttcccata gtcaatcgtt
     4441 gcaagtccac cacttccttg ccacagtagc ccaccatacg cttgatctga tgaaacttcc
     4501 cttctgcaat ggtcacacgg atttggcttt gattcttttc tgtatctatg gatacaagct
     4561 ccagtatagc gggttgacag gtaaagtctt tgagaggaat accctcagca aatgtctcca
     4621 catcttcttg ggtcatgatt cccttgactt gtgccagata agtcttgtcc acatgacgct
     4681 tgggcgaaag aagaacatga gccagctgac catcattggt caagagcaaa agaccatgcg
     4741 tgtcaatatc caagcgtcct actgggaaaa cttccttact ccgcgccaag tcatccaaca
     4801 agtccagaac ggttctgtgc ttgggatcct cagtcgctga gataactcct ttgggcttgt
     4861 tcatcatgta gtagacaaac tcttcatact ccaacacttg cccatcaaag cgaatctcat
     4921 ctattttttc atcaatctgc aatttagctg atttttcttt tttaccattt acagtcacgc
     4981 gcccagcctt gagcaagttt ttgacctcag tccgacttcc caccgcacag gcaactaaaa
     5041 atttatctaa tctcatagaa ctattatatc atatcaaaag gaggctggta caatgaccaa
     5101 cctccttttc gtttcatact cttcaaaaat ctcttcaaac cgcgtcaacg tcgccttgcc
     5161 gtatatatgt t
//
LOCUS       DAVKJD010000091         5085 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKJD010000091 DAVKJD010000000
VERSION     DAVKJD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.091"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(136..1698)
                     /gene="guaA"
                     /locus_tag="VW157_001795"
     CDS             complement(136..1698)
                     /gene="guaA"
                     /locus_tag="VW157_001795"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9553058.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFVLNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            1840..2538
                     /locus_tag="VW157_001796"
     CDS             1840..2538
                     /locus_tag="VW157_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9553059.1"
                     /translation="MLPAYMKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPERFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2581..3477)
                     /locus_tag="VW157_001797"
     CDS             complement(2581..3477)
                     /locus_tag="VW157_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9553060.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGTLMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLIIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYTIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3486..4421)
                     /locus_tag="VW157_001798"
     CDS             complement(3486..4421)
                     /locus_tag="VW157_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9553061.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4418..>5085)
                     /locus_tag="VW157_001799"
     CDS             complement(4418..>5085)
                     /locus_tag="VW157_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proteinase"
                     /protein_id="HEU9553062.1"
                     /translation="NETFYIETLGMKALLEESAFLSLGDQTGLEKLVLEEAPSMRTRK
                     VEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQNGYAFEIFSPEDDLILIHAED
                     DRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDIESFLESSEIGASLDFIPAQG
                     QDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYFIPKSEKFFLGKDRNNVELWF
                     EEV"
BASE COUNT         1573 a         1114 c          946 g         1452 t
ORIGIN      
        1 tttttgtcca aatgtaaacc acttggaact attattcaat ttattctttt tagtaaatgt
       61 tcccgtttta atcccagtta cagtttttaa tcctctgtaa ccgttgattt tacaaagctt
      121 ttaatttttt tgcgattact cccactcaac tgttgcaggt ggtttacttg taatatcgta
      181 gacgatacgg ttaacatgat ccacttcatt tacgatacgt actgagattt tttgaagtac
      241 ttcccatgga attttggcaa aatcagcagt cataccatcg atagaagtga tagcacggat
      301 tgcaatcgtg tagtcatacg tacgaccgtc acccataaca ccgactgaac gaacgcctgt
      361 gttaacagtg aagtattgcc aaatatcgcg gtcaagtcca gctttagcga tttcttcacg
      421 aagaatagcg tctgattcac gaacggtttc aagtttctct tcagtgattt cacccatgac
      481 acggatagca agtcctggtc ctgggaatgg ttggcgccat acgatatggt ctggcatacc
      541 aagctctgta ccaagagcac gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa
      601 ttcaaactgc atatcttctg gaagaccacc cacgttgtgg tgtgacttga tagtttgagc
      661 tgtatccgta ccagactcga taacatctgt atataaagta ccttgagcaa ggaatttcac
      721 atctttgagc ttgcttgctt cgtcatcgaa tacatagaca aactcgttac cgatgatttt
      781 acgtttttgt tcagggtcag aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc
      841 tgctttgacg atattcaaac caaacttacc accgagcatg tccataactt gatcagcttc
      901 gcctttacga agaagaccgt ggtctacgaa gatacagatc aattgatcgc caatcgcttt
      961 ttggagaaga accccaacga cagatgagtc aacaccacct gatagaccaa gaaggacacg
     1021 tttatcaccg acggtttcac gaattttttt gatctgcatg tcaatgaaat tatccattga
     1081 ccagtcacct ttagccttac aaatgttaag gacaaagtta cgaaggatat catttccgta
     1141 tacagaatga cgaacttctg ggtggaattg gataccgtaa atgtgtttat ctgggttttc
     1201 gatggctgcg tatgggcagt cagctgatgt acctgtacga acaaagtcag caggaatctc
     1261 agtaaccgca tcaccatggc tcatcaaaac agtctgttca tcaggtgttg attcaaaaag
     1321 cgctgatggt gtgtgagtta gggttgattg accgtattca cgatttccag catcacctgc
     1381 aggaacaact tttcctccaa gtttatgggt caataactgc ataccataac agattcccaa
     1441 aattggaatt ccgagttcga agatttctgg gtcaatatca aatgaaccat cttcatatac
     1501 agaatttgga ccacctgata gaataattcc tacaggattg acttcacgaa cttcagcagc
     1561 tgaaatttta tggcttttta gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat
     1621 cagctggttg tactggctac catagtccaa tacgatgatt ttttctacat cttgcaaatc
     1681 agttgaaatg ttgctcatct ttttcctttc tcaaaagaaa tatcttttcc caatattcta
     1741 tcacaaataa gggcgaatta ccaactaaac aaggcaaagt ttgacttttt ctagtttatt
     1801 ggggtacaat agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc ttatatgaaa
     1861 atccatgatc agataaaaaa ggatattgac gagtaccgtt gggctattgg tgagaggctt
     1921 ccaagtgaaa gagatttagc tgagcagttt gcggtcagtc gtatgaccct ccgccaagcc
     1981 gtatctctat tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg cacctttgtt
     2041 tccagtactc gagtacaaga aaagatgcga gggacaacca gttttactga aattgtcaaa
     2101 tcccaaggta aagttccctc tagccagctc atttcctaca aaaaaaccat tcccaatgag
     2161 caagaagttg ccaagctagg aatttttcca acggacaata ttatccgaat ggagcgggtc
     2221 cgctatgccg accaagttcc cctagtttat gaagttgctt ctattcctga aagattcatt
     2281 aaggacttta aaaaagaaga aatcaccagt catttcttcc aaaccttgca aaaacatggc
     2341 tatcgtatcg gcaaatctca acagaccatc tatgctcgcc ttgctaaaga aaagattgcc
     2401 cactatttgg aagttgaaaa aggacatgct attcttggat tgacccaggt ttcctaccta
     2461 gaagatggta ctgcttttga atacgtaaaa agtcaatatg taggcgaacg ctttgaattt
     2521 tatcttgaaa ataattagat taccacgcaa aaactctctg tcacggacaa agagtctttt
     2581 ttatttttct aagaataaat ctttcaaaga aatcaaagtt acagcaaaca atatcattgc
     2641 cataccaaga aaatcaatgg tataaaattg ttcattcatt attaagaagg caaagaaaac
     2701 cgccgatatt ggctcaattg aagccaacaa gcttgacttg accggtccta tcagactggc
     2761 tcctttaagg aaagctgtat aggcaaagac agtcccgata aggataatgc ctgcaaacgc
     2821 aaggagaaaa tcaagactag tcgggatatc ggcctgtaga acccctgtaa aaggaagggc
     2881 gaccaaacct gctatgacca ttcccacacc aatgatcaag ctgctccccc actttttaat
     2941 caaggctatg ggtaaaatga tatacagagc ataagtcaag gcagaaaaga gaccccagaa
     3001 caggccagca ggtgtcatgg ataactggtc caactgccca tgtgttgcga tcaggaaggt
     3061 tcctccgatg gcgaatatga tggaaactat ctctcccagt gtcggtgcca ccctatcctt
     3121 gatacagcta taaattaaaa ttccgacagg acaaacatac tgaagcaccg tcgctgttcc
     3181 cgcattggtc tcctgaatag cagacagata ggcgaattgg ttgagaaaaa gaccaatcag
     3241 agcaaaaata agaagagaca gcaaactctt tctatccttt aaaaagacca gtattttatc
     3301 ctttgcagta gcataagcca agagcatgag agttccacca gcgattaaaa gacgcaagtt
     3361 ggtcaagacc agagccgaaa ttccgtgtgc catcaggtat tggccactcg ttcctgacaa
     3421 cccccaagca ataccagcca caactgttag taaagtccct tttaaactat ttgacatgtc
     3481 tctccttact cttctttgcg aatcaagttc atcacttgat tgcgatctaa tatccattga
     3541 gctctctcgt ccttttcata agtacgattc gataggaaaa tagtggcttc ttgcttctga
     3601 cgattccaca tgataaaggt acctgtatag cccgtatggt ctagccaatc tccttccaaa
     3661 ttccatgcta aagaacgttc cttgtcatcc aaaggagaaa aattttgatt taagtctctt
     3721 gcaaaatcat ctgctaaata gtgttctaaa aagatttgta aatcctttat agtcgaaaat
     3781 aaaccagcac tcccagcatg tctacccagg agacgagcct tgggatcatg cactatgcct
     3841 gcctctacac ctctaactgt tggaacagca agctcaactg gcccaaactt agtttccgtc
     3901 attccccaag gtttccagac ttgatccttt aaaatcacat ccaaatcttg attaaaaatt
     3961 ctttccaaaa taaagcccaa cagcaaaaaa tggacatccg aataaagaaa ggctggctga
     4021 cttcgtctgt tgagatgaaa catcgcttcc tttaattcag gggctgttaa aagatcacga
     4081 ttaggaataa aaggatcaag gtctgttgca tgagtcaaga gctggcgaat agtgatgtct
     4141 ggataatcac tctcaggtaa aaaatctatt accagtctat caatatctaa ttgacctatt
     4201 tcccacaaga aggtacaaac tgtgccaacc ccaacaacct tgctgacact agctaggtca
     4261 taaactagtc ctgcctcagt ctgcaagcca tgctctgggt cactctggcc taaatagaac
     4321 tctgtccatt gattgtcctt aaaatacgca aaagaggctc cgggataaat tcctgcctcg
     4381 atttgttctt ctattttttt aataatcttg gtccacttca tacttcttca aaccacaatt
     4441 caacattatt tctatcttta ccaaggaaga atttttcaga cttaggaata aaatattcag
     4501 tagactcaaa cttctggcga agacttgcta tgtctaattc attgaccaag aacttgagca
     4561 tagataagtc ccaggtaacc gtattgtcca cagtcaaatc ctgcccctga gctggaataa
     4621 aatcaaggga tgccccaatt tcagatgatt ccaagaaact ttcgatatca gttgggagat
     4681 gtaattccat agaaatctca aatttactta aagaaattga tgccaaatct gtttgaaatt
     4741 caggcttttc tcctacttct actagacttg ctctgtcatc ttccgcatga atcaaaatca
     4801 aatcatcttc tggtgagaaa atttcaaaag cgtagccatt ttgaccttta tataatcgat
     4861 gaatcgaatc tgttttagat aagattcctt caatttctaa gggattttcc accttgacaa
     4921 tcaatctagc tagttttttt cttccctcta ccttacgagt acgcatactg ggagcttctt
     4981 ctaaaaccag cttttcaaga cccgtttggt cacctagtga cagaaaggcc gattcttcta
     5041 acaaggcctt cattccaagg gtttcaatat aaaatgtttc attta
//
LOCUS       DAVKJD010000092         5069 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKJD010000092 DAVKJD010000000
VERSION     DAVKJD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.092"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            42..1796
                     /locus_tag="VW157_001800"
     CDS             42..1796
                     /locus_tag="VW157_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9553063.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     TLLALTIIGGLILGAMMGEQEFMKIYQEALSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNEGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1780..3501
                     /locus_tag="VW157_001801"
     CDS             1780..3501
                     /locus_tag="VW157_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9553064.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            3512..4096
                     /locus_tag="VW157_001802"
     CDS             3512..4096
                     /locus_tag="VW157_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9553065.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            4096..4791
                     /locus_tag="VW157_001803"
     CDS             4096..4791
                     /locus_tag="VW157_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9553066.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            4779..>5069
                     /locus_tag="VW157_001804"
     CDS             4779..>5069
                     /locus_tag="VW157_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9553067.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVN"
BASE COUNT         1804 a          672 c          952 g         1641 t
ORIGIN      
        1 atttatatct aaaagggata ttaaagaaag gagatatgct tatgaagatt tacaaaaaac
       61 tatttgctta tgtccaagat aagaaatatc ttggggtttt ggccataatt ttttctgcta
      121 tatctgctct acttacagta tatggatatt atttaatcta caaatttcta gataagttaa
      181 taattaattc aaatttatcc ggtgcagaga gcatagcatt aaaatctgtt attacactaa
      241 caagtggagc gatattttat tttgtctcag gaatgttttc acatatcttg ggatttaggc
      301 ttgaaacaaa tttaagaaaa aggggaatcg atggtctgga aaaagcaagt tttaggttct
      361 ttgacttaaa tccatctggt caaataagaa agattataga tgacaatgct gcacaaactc
      421 atcaggtggt agcacacatg attcccgata gttctcaggc aataatcaca cccgtacttg
      481 tacttgcact tggctttata gtaagtataa gggttggtat aactttgctt gctcttacta
      541 taattggcgg cttaatttta ggggcgatga tgggcgagca agaatttatg aagatatacc
      601 aagaagctct atctaaacta agtgctgaaa ctgttgagta cgtgagagga atgcaagttg
      661 taaaaatatt taaagcaaat gtagagtctt ttaaaagctt ttataaggcg ataaaagatt
      721 actcaaagta tgcttatgat tattccctat cttgtaaaag gccttatgtt ttgtatcaat
      781 ggttattttt tggactgatt gcaattttaa ttattcctat agtttatttt atgactagct
      841 tagctagcgc aaaggtgatt ttacttgagc ttatcatgat tttattttta tcaggagttc
      901 tctttgtttc attcatgaga atgatgtggt actccatgta tatttctcaa ggaaattatg
      961 cagtagatac tttagaggcg ctttacgaag atatgcaaaa agacaaatta gtgcatggta
     1021 atgtcaataa ttttaaaaac tataatatag aatttgagaa tgttagcttt gcttataatg
     1081 ataaagctgt cattgaaaat ttatccttta atttagaaga aggaaagtcc tacgcacttg
     1141 tcggttcatc tggatcaggc aaatcaacag tagcaaaact tatatcaggt ttttacaatg
     1201 ttaatgaagg aagcataaag ataggcggga tagcaataag tgaatattct gacgaagcct
     1261 taattaaagc catttccttt gtttttcaag attcaaaatt attcaagaag agcatttatg
     1321 ataatgtagc tttagctaat aaagatgcga cgaaagatga cgttatgaga gccttaaaat
     1381 tagcaggatg cgatttaata ttagacaaat tcccagaaag agaaaataca atcataggct
     1441 caaaaggtgt ttatttatcc ggtggagaaa aacaaagaat tgcaattgct agagcaattt
     1501 taaaggattc caaaattatt attatggatg aagcatcagc atctattgac ccagataacg
     1561 agtttgaatt gcaaaaagct tttaaaaatc ttatgaagga taaaacagtt atcatgattg
     1621 cacacaggct atctacaatt aaagaccttg atgaaattat tgtcatggat agtggaaaaa
     1681 ttatagaaag agggtctgac aaagaattaa tgtcaaaaga tacaaggtat aagagcctgc
     1741 aagagatgtt taacagtgcg aatgaatgga gggtttcaaa tgaaagagtt ttataaaaaa
     1801 agatttgctc ttacagatgg aggagcaaga aatttaagta aagcaacact ggcttcattt
     1861 ttcgtttatt gtataaacat gcttcctgcc atattactta tgatttttgc tcaggaagtt
     1921 ttggaaaata tgggcaaaag caatggcttt tatatagtat tctcagtttt gactttgata
     1981 gcaatgtata ttttgctttc tatcgaatac gataaattat ataacacaac ctatcaagaa
     2041 agtgcagatt taagaataag gacagcggag aatttatcaa aattacctct atcttacttt
     2101 tctaaacatg acatttccga catttcacaa acaatcatgg ctgatattga aggcatagag
     2161 catgcaatga gccactcaat accaaaggtg ggcggcatgg tactgttttt cccattaata
     2221 tctgtaatga tgctagcggg caatgtcaag atgggtttag ctgtaattat tccatctatt
     2281 ttaagcttta tatttatacc tttatctaaa aaatatcagg ttaatggaca gaatagatat
     2341 tatgatgtct taagaaaaaa ctcagaaagc tttcaagaaa atatcgaaat gcaaatggag
     2401 attaaagcat ataatttatc gaaggatatt aaagatgact tatataaaaa aatggaagat
     2461 agtgagagag tacacttaaa ggcggaagta actacaattt taactttgtc tatatcttca
     2521 atatttagct ttatatctct tgctgttgtg atatttgtcg gcgtaaatct aattattaat
     2581 aaagagataa attctctcta ccttatagga tatttactag ctgctatgaa gataaaagac
     2641 tctttagatg catctaaaga gggcttgatg gaaatatttt atttatcgcc caaaatagaa
     2701 agattaaaag aaattcaaaa tcaagattta caagaaggcg atgactatag cttaaaaaaa
     2761 tttgatattg atctaaaaga tgttgagttt gcctacaata aagacgcaaa agttttaaat
     2821 ggtgtaagtt ttaaagctaa gcagggagag gtcactgctt tggtaggtgc aagtggctgc
     2881 ggtaaaacaa ctatcttgaa acttatatca agactttatg attatgacaa gggacaaatc
     2941 ttaatcgatg gcaaagatat aaaagaaata tcaacagaat ctctttttga taaggtctct
     3001 atagttttcc aagatgtggt tctctttaat caaagcatta tggaaaatat tagaatcgga
     3061 aagcaagatg caagtgacga agaggttaaa agagcagcaa aacttgcaaa ttgcacagat
     3121 tttatagaaa aaatggataa aggtttcgat acagttattg gtgagaacgg agctgagcta
     3181 tcaggaggag aaagacaaag attatcaata gccagagcct tcttaaaaga tgcgccgata
     3241 ttgatcttag atgagataac agcaagcctt gatgttaaca acgagaaaaa gattcaagag
     3301 tctttaaata atttagttaa agataaaact gttgtaatca tttcacatag aatgaaatcc
     3361 atagaaaatg cagacaagat agtagttctt caaaacggaa gagtagaaag cgaaggtaag
     3421 catgaagagc ttttacaaaa atcaaaaatt tacaaaaatt taatagaaaa gacaaaaatg
     3481 gcagaagaat ttatttatta ggaggactac aatggataat aaaaaattaa aagtaaaaga
     3541 tttagtaagt atcggtgttt ttggcgtaat ttattttgcc ttcatgtttg gagttggtat
     3601 gatgggcttg attccaatat tgttcttaat atacccgaca gtattagcca tagttgcagg
     3661 aactgttgtt atgttattta tggctaaggt tcaaaagcca tgggcactat ttatatttgg
     3721 tatgatatca ccacttgtga tgtttgcagc tggtcatacc tacgtagttg tggttttatc
     3781 acttatagta atgataatag cagaattaat tagaaagatt ggtaattata attcatttaa
     3841 atacaatatg ctttcttatg caatcttcag cacatggata tgtagctctt taatgcaaat
     3901 gcttttagca aaagaaaaat atatggagtg gtctttgatg acgatgggaa aagattatgt
     3961 tgatgtatta gaaaagttaa taacttatcc tcacatggct ttagtagcct taggtgcttt
     4021 cttaggagga attcttggag catatatagg caaggctcta ttgaaaaaac actttgaaaa
     4081 agcaggcatt gtataatgcc tactttttct tcaaatgctc cctttaatcc caatccgatc
     4141 tctaagatat tagtcgtatt tatcacaggc ttaactgtta tgcatagcat aaacatccgt
     4201 tttgagctgg caattgtttg tcttattggt attttgcttt atttgaatgg atacaaaaaa
     4261 acacttttca aatggattat attgtgtggg atactttact ccttgcctaa ttttatggtg
     4321 ttatctgaat taaaccctat agttaagatg tttttgagta tacctattgt tattagaatg
     4381 tttattttac catttatggc agcaagcttt atgataaaga cctcggatgt aggcgcaata
     4441 atttcatcga tggataagct taagatttca aagaatgtat ccatacctat tgcggttatg
     4501 tttagattct tcccatcttt taaggaggag aagaaaaaca tcaaaatggc tatgagagta
     4561 agagggataa gttttaaaaa cccagtcaaa tatcttgaat atgtttctat gccactactc
     4621 attatatcat ctaatatatc agatgacatt gcaaaagcgg cagaaacaaa ggcaatagaa
     4681 aatccaattg ccaagaccag atacattcgc gtaaagatac agctaattga ttttgtttat
     4741 gttttagcgg ttgctggact tattgtggga ggcttaatat ggttgaaata aaaaatttaa
     4801 gtcttgatta tggtgaagag catatattag atgatatatc actatccata gccgagggag
     4861 agtgcgtgct atttacagga aaaagtggaa atggtaagtc atctttaata aattcaatca
     4921 atggactagc tgtaaggtat gataacgcaa agacaaaggg cgaaataatt attgatggta
     4981 agaatataaa aaatttggaa ctttatcaaa tctcaatgct tgtttcaact gtttttcaaa
     5041 atcctaagac atattttttt aatgtcaat
//
LOCUS       DAVKJD010000093         5052 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKJD010000093 DAVKJD010000000
VERSION     DAVKJD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.093"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            185..1730
                     /locus_tag="VW157_001805"
     rRNA            185..1730
                     /locus_tag="VW157_001805"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1782..1854
                     /locus_tag="VW157_001806"
     tRNA            1782..1854
                     /locus_tag="VW157_001806"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1815..1817,aa:Ala,seq:tgc)
     gene            1979..4880
                     /locus_tag="VW157_001807"
     rRNA            1979..4880
                     /locus_tag="VW157_001807"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4957..>5052
                     /gene="rrf"
                     /locus_tag="VW157_001808"
     rRNA            4957..>5052
                     /gene="rrf"
                     /locus_tag="VW157_001808"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1420 a         1027 c         1489 g         1116 t
ORIGIN      
        1 gttgacaagt gaaagtggct gtgatatact aatatagttg tcgcttgaga gaagcaagtg
       61 acaaagacct ttgaaaactg aacaagacga accaatgtgc agggcgctac aacataagtt
      121 gtagtactga acaatgaaaa aacaataaat ctgtcagtga cagaaatgag tgagaactca
      181 aacttttaat gagagtttga tcctggctca ggacgaacgc tggcggcgtg cctaatacat
      241 gcaagtagaa cgctgaagga ggagcttgct tctctggatg agttgcgaac gggtgagtaa
      301 cgcgtaggta acctgcctgg tagcggggga taactattgg aaacgatagc taataccgca
      361 taagagtaga tgttgcatga catttgctta aaaggtgcac ttgcatcact accagatgga
      421 cctgcgttgt attagctagt tggtggggta acggctcacc aaggcgacga tacatagccg
      481 acctgagagg gtgatcggcc acactgggac tgagacacgg cccagactcc tacgggaggc
      541 agcagtaggg aatcttcggc aatggacgga agtctgaccg agcaacgccg cgtgagtgaa
      601 gaaggttttc ggatcgtaaa gctctgttgt aagagaagaa cgagtgtgag agtggaaagt
      661 tcacactgtg acggtatctt accagaaagg gacggctaac tacgtgccag cagccgcggt
      721 aatacgtagg tcccgagcgt tgtccggatt tattgggcgt aaagcgagcg caggcggtta
      781 gataagtctg aagttaaagg ctgtggctta accatagtag gctttggaaa ctgtttaact
      841 tgagtgcaag aggggagagt ggaattccat gtgtagcggt gaaatgcgta gatatatgga
      901 ggaacaccgg tggcgaaagc ggctctctgg cttgtaactg acgctgaggc tcgaaagcgt
      961 ggggagcaaa caggattaga taccctggta gtccacgctg taaacgatga gtgctaggtg
     1021 ttagaccctt tccggggttt agtgccgtag ctaacgcatt aagcactccg cctggggagt
     1081 acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg gtggagcatg
     1141 tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatccct ctgaccgctc
     1201 tagagataga gttttccttc gggacagagg tgacaggtgg tgcatggttg tcgtcagctc
     1261 gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccctattgt tagttgccat
     1321 catttagttg ggcactctag cgagactgcc ggtaataaac cggaggaagg tggggatgac
     1381 gtcaaatcat catgcccctt atgacctggg ctacacacgt gctacaatgg ctggtacaac
     1441 gagtcgcaag ccggtgacgg caagctaatc tcttaaagcc agtctcagtt cggattgtag
     1501 gctgcaactc gcctacatga agtcggaatc gctagtaatc gcggatcagc acgccgcggt
     1561 gaatacgttc ccgggccttg tacacaccgc ccgtcacacc acgagagttt gtaacacccg
     1621 aagtcggtga ggtaaccgta aggagccagc cgcctaaggt gggatagatg attggggtga
     1681 agtcgtaaca aggtagccgt atcggaaggt gcggctggat cacctccttt ctaaggataa
     1741 ggaactgcgc attggtcttg tttagtcttg agaggtcttg tggggcctta gctcagctgg
     1801 gagagcgcct gctttgcacg caggaggtca gcggttcgat cccgctaggc tccattggtg
     1861 agagatcacc aagtaatgca cattgaaaat tgaatatcta tatcaaatag taacaagaaa
     1921 ataaaccgaa aacgctgtag tattaataag agtttatgac tgaaaggtca gaaaataagg
     1981 ttaagttaat aagggcgcac ggtggatgcc ttggcactag gagccgacga aggacgtgac
     2041 aaacgacgat atgccttggg tagctgtaag taagcgatga tccagggatt tccgaatggg
     2101 ggaacccaac aggtagtacc tgttacccac atctgttaag gatgtgagga ggaagacgca
     2161 gtgaactgaa acatctaagt agctgcagga agagaaagca aaagcgattg ccttagtagc
     2221 ggcgagcgaa acggcaggag ggcaaaccga agagtttact cttcggggtt gtaggactgc
     2281 aatgtggact caaagattat agaagaatga tttgggaaga tcagccaaag agagtaatag
     2341 cctcgtattt aaaatagtct ttgtacctag cagtatcctg agtacggcgg gacacgtgaa
     2401 atcccgtcgg aatctgggag gaccatctcc caaccctaaa tactccctag tgaccgatag
     2461 tgaaccagta ccgtgaggga aaggtgaaaa gcaccccggg aggggagtga aatagaacct
     2521 gaaaccgtgt gcctacaaca agttcgagcc cgttaatggg tgagagcgtg ccttttgtag
     2581 aatgaaccgg cgagttacgt tatgatgcga ggttaagttg aagagacgga gccgtaggga
     2641 aaccgagtct gaatagggcg ccttagtatc atgacgtaga cccgaaacca tgtgacctac
     2701 ccatgagcag gttgaaggtg cggtaagacg cactggagga ccgaaccagg gcacgttgaa
     2761 aagtgcttgg atgacttgtg ggtagcggag aaattccaaa cgaacttgga gatagctggt
     2821 tctctccgaa atagctttag ggctagcgtc gacattagag attcttggag gtagagcact
     2881 gtttgggtga ggggtccatc ccggattacc aatctcagat aaactccgaa tgccaatgaa
     2941 ttatggtcgg cagtcagact gcgagtgcta agatccgtag tcgaaaggga aacagcccag
     3001 accaccagct aaggtcccaa aataattgtt aagtggaaaa ggatgtgggg ttgcacagac
     3061 aactaggatg ttagcttaga agcagctatt cattcaaaga gtgcgtaata gctcactagt
     3121 cgagtgaccc tgcgccgaaa atgtaccggg gctaaaacaa tttaccgaag ctgtggatac
     3181 ctttataggt atggtaggag agcgttctat gtgtgatgaa ggtataccgt gaggagtgct
     3241 ggaacgcata gaagtgagaa tgccggtatg agtagcgaaa gacaggtgag aatcctgtcc
     3301 accgtaagac taaggtttcc aggggaaggc tcgtccgccc tgggttagtc gggacctaag
     3361 gagagaccga aaggtgtatc cgatggacaa caggttgata ttcctgtact agagtatgta
     3421 gtgatggagg gacgcagtag gctaactaaa gcagacgatt ggaagagtct gtctaagcag
     3481 tgaggtgtga attgagtcaa atgcttaatt ctataacatt gagctgtgat ggggagcgaa
     3541 gtttagtagc gaagttagtg acgtcacact gccaagaaaa gcttctagcg tttaaacata
     3601 ctctacccgt accgcaaacc gacacaggta gtcgaggcga gtagcctcag gtgagcgaga
     3661 gaactctcgt taaggaactc ggcaaaatga ccccgtaact tcgggagaag gggtgctgac
     3721 ttaaagtcag ccgcagtgaa taggcccaag caactgttta tcaaaaacac agctctctgc
     3781 taaatcgtaa gatgatgtat agggggtgac gcctgcccgg tgctggaagg ttaagaggag
     3841 tgcttagcgt aagcgaaggt atgaattgaa gccccagtaa acggcggccg taactataac
     3901 ggtcctaagg tagcgaaatt ccttgtcggg taagttccga cccgcacgaa aggcgtaatg
     3961 atttgggcac tgtctcaacg agagactcgg tgaaatttta gtacctgtga agatgcaggt
     4021 tacccgcgac aggacggaaa gaccccatgg agctttactg cagtttgata ttgagtgtct
     4081 gtaccacatg tacaggatag gtaggagtct aagagatcgg gacgccagtt tcgaaggaga
     4141 cgctgttggg atactaccct tgtgttatgg ccactctaac ccagataggt gatccctatc
     4201 ggagacagtg tctgacgggc agtttgactg gggcggtcgc ctcctaaaag gtaacggagg
     4261 cgcccaaagg ttccctcaga atggttggaa atcattcgca gagtgtaaag gtataaggga
     4321 gcttgactgc gagagctaca actcgagcag ggacgaaagt cgggcttagt gatccggtgg
     4381 ttccgtatgg aagggccatc gctcaacgga taaaagctac cctggggata acaggcttat
     4441 ctcccccaag agttcacatc gacggggagg tttggcacct cgatgtcggc tcgtcgcatc
     4501 ctggggctgt agtcggtccc aagggttggg ctgttcgccc attaaagcgg cacgcgagct
     4561 gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc gcgggcgtag gaaatttgag
     4621 aggatctgct cctagtacga gaggaccaga gtggacttac cgctggtgta ccagttgtct
     4681 tgccaaaggc atcgctgggt agctatgtag ggaagggata aacgctgaaa gcatctaagt
     4741 gtgaaaccca cctcaagatg agatttccca tgattatata tcagtaagag ccctgagaga
     4801 tgatcaggta gataggttag aagtggaagt gtggcgacac atgtagcgga ctaatactaa
     4861 tagctcgagg acttatccaa agtaactgag aatatgaaag cgaacggttt tcttgaattg
     4921 aatagatatt caattttgag taggtattac tcagagttaa gtgacgatag cctaggagat
     4981 acacctgtac ccatgccgaa cacagaagtt aagccctaga acgccggaag tagttggggg
     5041 gtgccccctg tg
//
LOCUS       DAVKJD010000094         4965 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKJD010000094 DAVKJD010000000
VERSION     DAVKJD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.094"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..400)
                     /locus_tag="VW157_001809"
     CDS             complement(<1..400)
                     /locus_tag="VW157_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9553068.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIP"
     gene            complement(397..1254)
                     /locus_tag="VW157_001810"
     CDS             complement(397..1254)
                     /locus_tag="VW157_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9553069.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRIKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(1258..2604)
                     /locus_tag="VW157_001811"
     CDS             complement(1258..2604)
                     /locus_tag="VW157_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9553070.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATALIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(2617..2901)
                     /locus_tag="VW157_001812"
     CDS             complement(2617..2901)
                     /locus_tag="VW157_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9553071.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(2905..4935)
                     /locus_tag="VW157_001813"
     CDS             complement(2905..4935)
                     /locus_tag="VW157_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9553072.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQISSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
BASE COUNT         1679 a         1050 c          684 g         1552 t
ORIGIN      
        1 taggaattaa acgtaacaat ccaatttctt caaatggcat atgtgtccca ccattcattt
       61 ctgcagttac tcctgcatct gatccaatca cagtggcatc caattgtgcg tatccaagag
      121 aaataaataa ttgatcaaat actcttcgtg aagcaaaagg accaaatgta tgaagataag
      181 gtctaaaccc ctgaatagac aagcctgctg caaggccgac catttctgct tccataatcc
      241 caacattcac ataacggtct ccaaagtcct tttcaagatt atgagtagcc atcgaacttg
      301 acagatcggc ttctaagact actatatcag aataactttg attagcctct aaaaggaagt
      361 ctctatatac atgccgtaat tctttcgtac ttctcatcat tctgtttcct ccaattcctg
      421 acttaatctt tctacaactg aagttaacat ttgtctctcc tctacagtag ggcgaagatg
      481 atgattggat ttcatttctt ccagctcttg aaccccttga cctttaatag tatctaatac
      541 aatgcactta ggtgatgaat tatttgactg ttttaattgg acaatccctt cataaatttc
      601 tctaatatct gaacccttga tcctaatgga ttcaaatcca aatgctgaaa atttttctac
      661 gaaatcacct ggattacaaa tatcctttgt aaaaccatct aattgttttt tgttatcatc
      721 aacaaataca attaagttgg ataactgttg atgagaagca aactgtatag cctcccaaca
      781 ttgtccctca tttaactcac catctccaac aatagcataa gtataaaagg gactctttct
      841 tattctctga ccatatgcaa gtccagttgc aacactaatt ccttgtccta aagagcccgt
      901 tgtcatatct atgcctggcg ttagatttct atcaggatga gacggtaatt tggttccatt
      961 tgtatttaaa gaatataaga attctttgtc aaagaaacca ttcaaataga gtgtactgta
     1021 tagagctggt cctccgtgac cttttgataa tatgaaataa tctctatctc gtgctgcaaa
     1081 tatttctgga gtcattggca ttatttcacc ataaagcact gctaaaactt ctacgataga
     1141 cagacttcct ccgtaatgtc cgaatccaag atgattcaat gttctaagag tatttaatcg
     1201 gatgttagtc gcaaattttc ttaactcatc ttccctattt ttacttaaaa tcatccctta
     1261 ttcctccttt gcagatggct ttttaataaa ggatactcca aacataactg ctagaataag
     1321 aacaagacca atcacaatgc ctgcttgtga gccaaattga tttaacattc ctaaaataat
     1381 tcctgataga ccaaaatctg catctgagaa agttgatcct tggaaaccaa gtcctcccaa
     1441 aactggcatt aaaaagactg gaagaaaact gattaaaata ccttgtaaaa atgctccaat
     1501 agtggctcca cgaacaccac cagatgcatt cccaatgaca cctgcagtcg ctccacagaa
     1561 gaaatgaggc acaacacctg gtaagataac aaccgttcct gaagcaatca taattgccat
     1621 acttactaaa ccaccaacaa aactagagat aaatccaatt agaactgcat tgggtgcata
     1681 agtataaaca atcggacaat ccaaagcagg ttttgaatta ggtacaagac gctctgaaat
     1741 acctttaaag gctggaacaa tttcgcccaa aataaggcga acacctgcta aaataacaaa
     1801 tacccctgct gcaaattgac ctgctaattg taaagcataa actagaccac ttgtaccact
     1861 actgatttct ttttctatat attctgaccc tgcaaagata gctacaataa tgtaaataac
     1921 tgccatggat aaagtgatac taacagtact atcacgtaaa aaagctaaac tctttggaaa
     1981 tttaatgtcc tctgttgatt ttgatttgtc accgataagg ctaccagtaa aaccactcaa
     2041 ccaatatccc aaagaactga aatgacctaa agccaccttg tcatttccag ttaattgaac
     2101 catatatttt tgaacaaatg ctggggaaat actcataata ataccgagtg ctaatcctcc
     2161 tagtaagata agaggcaagc tagtaaagcc agcaactgat aaaatgaccg caatcataca
     2221 tgccatatat agagtgtggt gccctgttaa aaaaatatat ttaaatcgag taaaacgagc
     2281 gattaagata ttgaacacca tgcctgcaaa cataatcagt gcagtagctg agccatatgt
     2341 tgttaaagct acagctacaa ttgcttcatt attcggcaca acgccagata aatgaaaagc
     2401 atgctcaaac atggtaccaa atggatttaa agaattttgt acaattcctg caccaccaga
     2461 tacaactaag aaaccaacaa aggtcttaat tccaccttta ataatatcag gtaatttctt
     2521 cttctgaaga actaatccta aaattgcaat taaagctact aaaatagctg gtgtactaac
     2581 aatatccaat atgaacttca tcataacgct agcctcctat ataagtcctt tttcttcaca
     2641 aagtttagta attaattctc gtagttcatc catatcaata atactattta agatacgaac
     2701 atctccaaga tgactagctg aatcagctag atcacgacca acaatccaaa tatcagctgc
     2761 atttggatct gctccaccta aatcataatg ttcaacttct acatccgaaa cattcaaatc
     2821 actcaataca gattcaatat tcatctgtac cataaaactt gaacctaatc ctgaaccaca
     2881 agctgtacca atttttaaca ttatctaatc ctcctgttta attatcattt taatgtcatc
     2941 atagtttttt gatgatatta aagtttgaac atgattttta tctcttaaaa ttgttgttaa
     3001 atgtgacaaa gcctttaaat gactctcatt atcaatggct gcaatacaaa tcaacaatct
     3061 tacctcttgt tctggattat ccaataaata aatcggttct tccaaaacta acattgacat
     3121 tcctatttca ttcacacctt catctggccg agcgtgagga attgctactc ccttccctaa
     3181 attaataaaa ggcccaaact cttctacttt ttgaatcatt gcctcagggt agttctcagt
     3241 tatcttatct tgatccaaaa gcggtttagc tgctaaacga atcgcctcct tccatcctaa
     3301 tttttgcgaa ctaatctgat aggtttcttt ggtaatgagt tgttctagca ctggtacaat
     3361 ttcctttcta tcattttttt ggtaaagata attctttaac gccaatctta attccaattc
     3421 ttgtgtaata attccatatc ttttgacaat attcaggatt tgttcaatct caaaatcccc
     3481 atactctaaa ttcggaaaat cttttaacac tagttctact agttgtattg cttgctcttc
     3541 agtcatcata accgaaacta gataatttgg cttttctgtc tccaccttta tggtagaaaa
     3601 aaccatatca tagtcactac tagctttcac ctgtaaatca tcaatctttg aggttcctat
     3661 aaactcaatt tgaggaaata atgctaatag attctctttt aacatcaatg aagaactaac
     3721 accattagga caaatgattg ctgctttata gcatttttga ggcaaagtat ctgctttctt
     3781 taaataacct ccgaaatgga taacaaaata tgctgtttca ctatcaggta tgggattgtc
     3841 aatagcgtcc atcaagggca tcaaagaatc tttgactaat tcaaataaat caggataatg
     3901 ttctttaaca tgcaatacat attcatttga actaggtagg ccgaacttta atctatagta
     3961 agccggtata aggtggcggc gaagattttc tctcaatcct tccctttgtt taaaatgtaa
     4021 caaagaaata tcttccattc tacttataat agcctctgtt aattgattaa agtaaaccgg
     4081 agcaacatct acttcacctt caaagcaact tgataataaa acggtgatat agcgataatc
     4141 atcctcagaa aacaccgtat ctataattcc caaatcaacc actgtatcca ataaaatagt
     4201 cgttatatct tgaataacag gagatactaa tgtctctgaa agacatactc tttcaacatc
     4261 cctttgatac ctacacagaa tgaatactaa accgaaaagg caaactttta attgattaac
     4321 aataggtact agctgtagct tctcataata atctttaact acctgattta tcaaatcata
     4381 agttaatgaa tacccccaac tggataaaac ataatccaaa ccccaaatcc ctatggagga
     4441 ttccagcaac tcactaacca tttgaaaagc taagcggtgc ttattccact ctgaaccgtg
     4501 taaagtataa ccttttgctc tactgtaccc tagctccaaa tcattatcta acataatctt
     4561 tcttaatgat tgaatatcag ataaggttgt attcttactt actttcaaaa agtcttggta
     4621 atgattattc gatataaaat ctaatcggca aaaagtgtaa agatagatta aagctaagcg
     4681 agtcgacttt ggtaaaacca attcatccga cttaataata tctgtcaaag actgcttcgt
     4741 acgatttgat aaactatagc gaccttgctt tttatccagc actatccctt tattagctag
     4801 ataaggcact aaataatcta ttccttcttt gacttccttt ataggtaagc tcaccttaac
     4861 agataattca tataatgata gctcacaatg atccatcaaa gtcatcaaaa taactagtgc
     4921 tctataatca aacatttctc ttcctttcta actacaatta tagtg
//
LOCUS       DAVKJD010000095         4957 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKJD010000095 DAVKJD010000000
VERSION     DAVKJD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.095"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(173..1588)
                     /locus_tag="VW157_001814"
     CDS             complement(173..1588)
                     /locus_tag="VW157_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9553073.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDITLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1601..3439)
                     /locus_tag="VW157_001815"
     CDS             complement(1601..3439)
                     /locus_tag="VW157_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9553074.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQIY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIAWPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3457..4296)
                     /locus_tag="VW157_001816"
     CDS             complement(3457..4296)
                     /locus_tag="VW157_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9553075.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(4596..4778)
                     /locus_tag="VW157_001817"
     CDS             complement(4596..4778)
                     /locus_tag="VW157_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9553076.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLELASIVSELVAGLR"
     gene            4838..>4957
                     /locus_tag="VW157_001818"
     CDS             4838..>4957
                     /locus_tag="VW157_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9553077.1"
                     /translation="MKLSCDDKVQIYELRKQGYSLEKLSNKFGINNSNLRYMIK"
BASE COUNT         1672 a          953 c          653 g         1679 t
ORIGIN      
        1 accaatctat cctaagttaa ctatttataa tcggctctct gtaacaaaat taaattaatt
       61 aatcccccta taaaataaag aggtttagaa tttgatgtct tccaaacctc tttatttcat
      121 atttaatgaa atgccacctt aaccgtgata atagctagtc atcaataaaa aactatttga
      181 ataaggattc tccatttgat tcaatcactc ctttatacca agtaaaggac attttcttat
      241 atcgattgaa tgtgccattt ccatcatcat ttcgatcaac ataaatgaga ccgtacctct
      301 tagaaagctg tgcagtggac atagaaacac agtcaataca tccccaagac gtatagccca
      361 taatttcaac accatcctgt agagcttcag caacttgcaa taaatgttct ttcatatact
      421 gaattctata atcatcttgg acggttaagt tattaagttc atcttttatt agttgatctt
      481 tagcacctaa tccattttct actataaata atgggatttg ataacggtca taatatctat
      541 ttaaaattat acgtagtcca attggatcaa tttgccatcc ccactcagaa gactctaaat
      601 aaggatttac taaaccacca ataatattcc cttctcctga attatactgt gttggaagag
      661 cagattgagt cacactcatg taatagctaa aggataaaaa atctacagta taatttttta
      721 ataactctgc atcttcagct gcaaactcta tgttaatgtc atgttcctta aaatatcttt
      781 ttgcataatt cggataataa cctctaacat gcacatctga aaatagataa tttagattct
      841 catactcatg agtcgcccat acatcttttg gatttggagt cattggataa gctggcatag
      901 ctaataccat acaccccacc ttaaactctg aattaatctc acgagcaatt tttgtaacca
      961 aactcgaggc gactaattca tgatgtatag cttgatataa ttcttgtttc gaaagattct
     1021 ccttaggtat atctattcct ccactagtaa acggtaattc caaaacagag tttacttcgt
     1081 taaatgtaag ccaatattta actttatctt tatacctttc taaaactgtt cgagcaaatt
     1141 tttcataaaa atgaatcatt ctcctatcaa tccatccatg atattttctt gctaaatata
     1201 atggagtctc atagtgtgaa agagttacaa gtggttctat cccgtgagca tgtagttcat
     1261 caaacaattc atcataatat ttcaacccag cttcgttagg ttcttcctca tctccttttg
     1321 gaaaaattct actccatgca atagaagtac gaaaaacatt aaagcccatt tcagaaaaca
     1381 aggttatatc ttccttatat ttatgataaa aatcaatacc tatcaatttt aagttatctt
     1441 ctgtaggatt ttctgttgct tctcctaatc cacctttggg taacacatcc tgaactgata
     1501 agcccttacc atcttcatta tatgctccct ctacttgatt agctgcaaca gctccacccc
     1561 aaagaaaatc atctggaaaa atggtcataa ctttcctcca ttataatatt accagtaatt
     1621 ccttagaatg ctcgattgtc tgattattag gtaatactaa tacatctaga aaatcattgg
     1681 tattcgttac aattactggt gtaactgttt cgtagccttt agtcttgatt aaattcaagt
     1741 ccatttcaaa aatcaactga tttttgaaaa ctctgtctcc ttcttctaca tgactaataa
     1801 aaccttgacc ttttagctca acagtatcta atccaatatg aattagtaac tcaacaccct
     1861 catcactctt caatccaatt gcgtgcttag tcggaaaaat atttgtaatt ttcccatcaa
     1921 atggtgcata aaccttacct tcacttggga taatcgctac cccgtctcca attagtttat
     1981 ctgaaaatgt tttatcctgg acatcgctta acggaatgat ttctcctgat ataggagaaa
     2041 atatcatttt tttatttgaa actccagctt taacttctaa attgctagaa ctctcttctt
     2101 catcgattcc aaatatataa gctaatacaa aggtaataac aaccgaaata accgccacaa
     2161 ttaaagcatt tacaatattt gatggtacat cagaataaat aaattgaggc cacgctatca
     2221 aagatgggac agcaaataga tacgctttaa cactagtaag acctgcaaat aatcccgcta
     2281 atccaccacc aatcatagct gcataaagcg gttttttata ttttaaagtc acaccatata
     2341 atgcaggttc ggtaatccct gcaagtaagg ctgagaaacc tgctgcaaaa gcaatttgtt
     2401 ttgtattatt atttttactc tttaatgcaa cagccatcga agcagcccct tgagctaagt
     2461 ttgaccctaa cattgctgga agaattaata cgtctggagt agcaatagat gccgccaaaa
     2521 aaataggtgc aaaagcccaa tgcattccag tcataacaat aaatggcata atagcaccaa
     2581 gaatagctaa tgtaagccat ccagctacac catatatttg cccaactaga tttgataatc
     2641 cttcaccaac aattactcca ataggtccga ctacaactaa ggcaatacag cttgatacta
     2701 ataatactag cgtaggttgc aaaaaactct tagtaatagc tggtgttaat ttagcaatta
     2761 ttttttcaat atatttcatc aaccaaacca taataagaat tggaacgact gatgaaccat
     2821 aactagctgg tgtcacaggt gcaccaaata aactaagagg attccctgat tgcaccattt
     2881 gaacaaaatt tggatggaga agtacacctg ctacagacat agctaatgta gatgttactt
     2941 ttaatttttg tgatgcagaa taagctaata acagcggtaa gaaataatat ggagcatccc
     3001 caaaaaatgt caaaaaagca atagtctgag aatctgattg caatatacca agcattggta
     3061 aaatgattac caagactttc aacatacctc cccctaacat tgctggaatg attggagtca
     3121 tggaaccagc gatatactca atgattcttt ctaaaatatt ccctttgtgc ccttgaacaa
     3181 ctgaatcgga ttcaaaattg ccaagtttaa cgaattcttt ataataatta gctacatcat
     3241 taccaagtat aatttgatat tgtccattct ttttcataat acctattaca cctggtatct
     3301 tcttcacatc atcatcattg actaaatttt catcttttaa ttctaatctt aaacgtgtta
     3361 cacaatgggt aactctattg acattttttt cacctccaat tacatcgagg attttttgta
     3421 ccgtatcttt ataactcatg gtattctcct attctattaa tctaaatttt ttgttaagcg
     3481 acgaatatga gccatcaaat aaactaattc actagaagtc agcaaataat tgtactcagt
     3541 ttgtataaac attgccacct gttcaccaca ttcatattct ctaggatatt tatttttcat
     3601 taatgctaac aagtcttcat catcatcgtc acaataagtt gtattttcaa ccaagcgatg
     3661 ggcaaataat tttatatgag ttatgaatct ataataatca atagaatctt ggttaaactc
     3721 agtacaatag aaatcttgaa tgattttctc aattcccata attatttcag tgattttata
     3781 cgactcttga aaattatttt ctaaattagc attaacaaaa tgtagcgcta tgaatgcagc
     3841 ttcgtcaatt ggaagttcaa tacctaactc tttttttatt atttgtaaag cccttttccc
     3901 aagctcaaat tcttctttat aatatttcga gatttcccag cgtaggggat tcgaaataat
     3961 aactccttct ttataacgtt ctatactcga agaaatatga tccgttagat ttatatagat
     4021 aatctcatca aaattattac ctaatgttat tttacctaga ttaattattt tttcggtgat
     4081 gtacaccaac tccaaaggaa cgttaataaa catctctgta aaacgggtca tattatctct
     4141 atttttgaga aaaaaacttt tctcaatatc aattggattt actttatctc caatttttct
     4201 tccaaaagct attccccttc caaaaagaag aatatcgata tcttttttac tttgcgcaat
     4261 tacggcattg tggtttagta ttcgttttat gatcataaaa tttccctttt aaaataaaag
     4321 gaacctactt aaaaacatca agaaacttct tctgatgtga ttaaataggt tcctgctcac
     4381 ttgtattagt gataacatcc ttttatttca atttttaatg gtgacatgaa gcttaaataa
     4441 ttaataactc tgtaaccatt ttcattttaa gatatttcaa tctatttgtc aagtacttgt
     4501 agaaattttt ttattttgtg tgattcgaat gtgataatgg caacagtctc actaaagaaa
     4561 tgaaaatcat acatagaaat ttttaaaaat tttctctatc gtaaacctgc cactagttca
     4621 gaaactatac tagctaattc caaacgtaat atggtcatct catctacctt ccctaataat
     4681 ggcattgcac cataccaaac aatagtctta ttcaccaaaa gaataggcgt tactattttt
     4741 gattgagtaa aagacacttt aattttctgg ctcttcaact gtgaactgca ccccaaaagt
     4801 tagacagaat aaatctaact tttggggtgt ttttattatg aaattgagtt gtgatgataa
     4861 agttcagatc tatgaactta gaaaacaagg atatagctta gagaagcttt caaataaatt
     4921 tgggataaac aattctaatc ttaggtacat gattaaa
//
LOCUS       DAVKJD010000096         4929 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKJD010000096 DAVKJD010000000
VERSION     DAVKJD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.096"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            105..701
                     /locus_tag="VW157_001819"
                     /pseudo
     CDS             105..701
                     /locus_tag="VW157_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            777..1226
                     /locus_tag="VW157_001820"
     CDS             777..1226
                     /locus_tag="VW157_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9553078.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVGLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            1239..2330
                     /locus_tag="VW157_001821"
     CDS             1239..2330
                     /locus_tag="VW157_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9553079.1"
                     /translation="MSEIEIINAKKIYHDVSVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNLSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKGNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            2320..3965
                     /locus_tag="VW157_001822"
                     /pseudo
     CDS             2320..3965
                     /locus_tag="VW157_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4068..4760)
                     /locus_tag="VW157_001823"
     CDS             complement(4068..4760)
                     /locus_tag="VW157_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9553080.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1544 a         1039 c          857 g         1489 t
ORIGIN      
        1 gtgtactgtt atagattcaa tacactatag aatagaattg taggttttaa aaacgcttac
       61 attgttttaa aataaactta aataacacaa cggaggtatc catcatgaaa aagaaatgga
      121 tgtattatgc tgcttgttct tctaatgaat ctgccgatga cagttcatct gataaaggag
      181 acggtggttc gctagtcgtt tattgaccaa actcagaagg cttaattgga gcaactattc
      241 ctgcctttga agaaaaatat ggtatcaaaa tagaactgat tcaagctggt actggagaac
      301 ttttcaaaaa actagagtca gaaaaagaag ttcctgtagc tgatgttatc tttggtggtt
      361 cttatacaca atatgctacc cacggagaac tctttgaaaa ctatatttca aaagaaaatg
      421 ataatgttat caaagaatat caaaacacaa ctggcttctc tactccttat acactagatg
      481 gtagtgtttt aatcgtccac cctgatttaa ctaaaggcat gaacatcgaa ggatatagcg
      541 accttctcaa acctgaacta aaaggaaaaa tcgcaactgc tgacccagca aactcttcta
      601 gcgcctttgc tcaattaaca aatatgctac aagctcaagg tggttacaaa gatgataagg
      661 cttggtctta tgtaaaagat cttttcacac ttattgatgg tatagtaaaa tgaaataaga
      721 acaggacaaa ttaatcagga cagtcaaatc gatttctaac aatgttttag aagtagatgt
      781 gtactattct agtttcaata tactataaaa tcggtttaag ttcatctggt gtctataaag
      841 cagtcgctga tggagaaatg actgttggtc tctcttatga agatccagca gttaaactct
      901 taaatgacgg agctaacatt aaggtagtct atccaaaaga aggaaccgtc ttcctacctg
      961 ctagtgctgc tatcgttaaa aaatctaaaa atatggaaaa tgccaagaaa tttatcgatt
     1021 ttattatctc tcaagaagta caagatacac ttggtacaac cactactaac cgtcctgttc
     1081 gtaaaaatgc taaaacaagc gaaaacatga aaccaattga caaaatcaaa acactcactg
     1141 aagattatga ttatgtcatc aagaataaat cagatatcgt taagaaatac aacgaagtct
     1201 ttacagatat ccaatctaaa cagtaaaaga ggttcactat gagtgagatc gaaattatta
     1261 acgccaaaaa aatctaccac gatgtctctg ttattgagaa tttgaacatt acaattccaa
     1321 aaggaagtct ctttaccctt cttggagctt caggatgtgg gaaaacgacc cttcttcgta
     1381 tgattgcagg tttcaacagt atcaaagatg gagaatttta cttcgatgat acaaaaatca
     1441 ataatatgga acccagcaaa cgcaatatcg ggatggtttt ccaaaactac gctattttcc
     1501 cacatttgac tgtccgagac aacgttgctt ttggtcttat gcaaaagaag gttccaaaag
     1561 aagaattgat tcaacagacc aacaagtatc ttgaactcat gcaaattgct caatatgcgg
     1621 atcgaaagcc cgataaactc agtggtggac aacaacaacg tgtcaccttg gcatgcgcct
     1681 tagcggttaa tctaagtgtt ctcctcatgg acgagccact tagtaatctg gaggccaaac
     1741 ttcgcttgga tatgcgtcaa gccatccgag aaatccaaca cgaagtggga attacaactg
     1801 tttatgtaac ccacgaccaa gaagaagcca tggctatttc agaccaaatt gctgttatga
     1861 aagatggggt gatccaacaa atcggccgac caaaagaact ctatcataaa ccagctaatg
     1921 agtttgtggc aacctttatc ggacgcacaa atattatccc tgccaatctt gaaaaacgga
     1981 gcgacggcgc ttatatcgtc ttttcagatg gctatgccct tcgaatgcta gctcttgatc
     2041 aggttgagga gcaagctatt catgtaagca ttcgtcccga agagtttatc aaagatgaat
     2101 ctggagatat tgaaggaact attagcgata gcgtctatct tggactaaat acggagtatt
     2161 tcattgagac aggttttgcc tcaaaaattc aagttagtga agaatcaact tttgaagaag
     2221 atctacaaaa aggcaatcgt attcgtctac gaatcaatac gcaaaaatta aacatctttt
     2281 ctgcagatgg ttcccaaaac ctgataaaag gagtcaacca tggaacgtaa aaaactaaat
     2341 atttggacag cctcctcttt cttcatcttt cttacctatc ttgtctttct cgtttatcct
     2401 atcgttaccg tgctcaagta agcacttata catgaaggac aattctcact agctaatttt
     2461 gtcactttct ttagtaaagt ctactactct gagacactag tcaacagttt caaggtttcc
     2521 attaccgcta ctgtcacttc cttagttgta ggaaccctat tagcttatct cttctctatg
     2581 tatgacttca agggggagaa atttctacaa atattgatta tcattgcttc catgtcagct
     2641 cctttcgtag gagcctactc ctgggttctc ttgctaggac gaaatgaggt catcactaaa
     2701 tttttgacaa atgcccttta tcttccagct atcgatattt atggattcaa aggaattgta
     2761 cttgtcttta cactgcaact attcccactg gtatttctat atgttgctgg aacaatgaac
     2821 agtattgaca attctctact tgaagcagct gaaagcatgg ggtccttcgg atttaagcgt
     2881 atcgtaacgg ttgttttacc tctcctagtt ccaaccttac tagcagctcc ttgcttgtat
     2941 ttatgagagc attctcagac tttggaacgc ctatgttgat tggcgaagga tatcggactt
     3001 tccctgtcct gatttatacc caatttatta gcgaggttgg aggaaattct gcttttgcaa
     3061 ttatggcgat tatcattgcc ttggcaattt tccttatcca aaaacacatt gcaaaccgct
     3121 acagtttcag catgaatctg ctccatccaa ttgagcctaa aaaaactaca aaaggaaaaa
     3181 tggctgccat ttatgcaaca gtctacggaa ttatctttat ctctgtttta cctcaaatct
     3241 acttaattta tacctctttc ctaaaaacat caggtatggt atttgttgaa ggttattctc
     3301 caaacagtta caaggtagct ttcaatcgta tgggatctgc tattttcaat accattcgta
     3361 tccctttgat tgccttagtt ctagttgttc tatttgcgac atttatctcc tacctagccg
     3421 ttagaaaacg gaatttgttt acaaacttaa ttgacagcct cagtatggta ccttatattg
     3481 taccaggaac cgttctaggg attgccttca tttcttcctt caatactggt ctatttggaa
     3541 gtggatttct tatgattaca gggactgctt tcatcttgat tatgtctcta tctgtcagaa
     3601 gattacctta tactattcgc tcatctgttg ctagcttaca acaaatagca ccaagtattg
     3661 aagaagctgc tgaaagctta ggaagtagtc gtctcaatac ctttgctaag attacaactc
     3721 caatgatgct atctggtatc atttctggag ccatcttatc ttgggtcaca atgatttcaa
     3781 aactctctac ttctatcctc ctctacaatg tcaaaacaag aacaatgact gtagctattt
     3841 atacagaggt tctcagagga aattacggtg tagccgcagc cttgtcaact atcctgactg
     3901 ttctaacagt aggttccttg ctcttgttta tgaaaatctc taaaagcaat agcattacac
     3961 tttagttttc cacatcaaaa acagccgaaa ggagtaccct cggctgtttt ttcttatctt
     4021 gatatcctta tcaaatccct agctcatggc aagcaagtct agactgatta aatagagcct
     4081 tcttccatct tctcacgtcc tagctctcgg taactacggt caccgacaac ttcaacccta
     4141 aactgaccgt cctcatattc aagaatcgtc acgctaccat tatctagacc atgcggatgc
     4201 atgccattaa tcagataaac aatggttcca atagtcattc catggctgac aacaagggcg
     4261 ttacctccac cttgatcttc catttctttt gcaattattt caaagccttc cttgattcgg
     4321 ccactgagtt tttcccagcc ttcagcccaa ccagctgtat cgacctctac caagccctca
     4381 gccagttcag cataagacaa ttggtgaacg tggtccacat taaagatacg aggaataatg
     4441 cccatgaaaa gatcgccatc ataggctcca tcaaaactac cgaaacacca ttctctgata
     4501 cgcttgtcca tgcgataagg gattttcccc tgcaagccaa gttcttcaag tataattccc
     4561 atggtctgaa tggtacgacc agaatcactc gaataagcgc gctcaaactg tagatcagat
     4621 tctcgcaaac cgattcctaa ctcttgaatc cctcgttcac cttcagcagt taagggagta
     4681 tcgctccaac cttgcgcgcg accaatcgtg ttaaacatgg tcttgccatg acgtaccaaa
     4741 tacaatcgta cttttaccat tttccgtcct ccgtttgctt ctattatatc atgaatgata
     4801 aaataaaagc cacccataca ggcgactttt gaaggagatt attatgaaaa agtttaggag
     4861 attattatga aaaagtttag gagttcatta aataaagata ttagatgaaa atcaaattca
     4921 aactaattc
//
LOCUS       DAVKJD010000097         4917 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKJD010000097 DAVKJD010000000
VERSION     DAVKJD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.097"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            179..442
                     /locus_tag="VW157_001824"
     CDS             179..442
                     /locus_tag="VW157_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9553081.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            448..2181
                     /gene="ptsP"
                     /locus_tag="VW157_001825"
     CDS             448..2181
                     /gene="ptsP"
                     /locus_tag="VW157_001825"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9553082.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKAHLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            2436..4886
                     /gene="phtA"
                     /locus_tag="VW157_001826"
     CDS             2436..4886
                     /gene="phtA"
                     /locus_tag="VW157_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEU9553083.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSHMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
BASE COUNT         1656 a          934 c         1040 g         1287 t
ORIGIN      
        1 aaactatatc ctgtgttttg ttatctagga tagaagattt tcagcaagtt atctgaaagg
       61 aaattgaagg aaaatccaga aaatgcttga aaaaaatcct agaagatggt ataatactaa
      121 attgtaaggg ttatcacata taactcaaaa aaagaaagaa caaaaggaga gtcaaactat
      181 ggcttctaaa gatttccacg tagtggcaga aacaggtatt cacgcacgtc cagcaacatt
      241 gttggtacaa actgctagca aatttgcttc agatatcact cttgagtaca aaggtaaatc
      301 agttaacctt aaatcaatta tgggtgttat gagtcttggt gttggtcaag gtgctgacgt
      361 aactatctca gctgaaggtg cagatgcaga tgacgctatc gctgcaatct cagaaacaat
      421 ggaaaaagaa ggattggcat aagggaaatg acagaaatgc ttaaaggaat cgcagcatct
      481 gacggtgttg cagttgcaaa agcatatcta ctcgttcagc cggatttgtc atttgagact
      541 attacagtcg aagatacaaa cgcagaagaa gctcgccttg atgccgctct acaggcatca
      601 caagacgagc tttctgttat tcgcgagaaa gcagtaggta cgctcggtga agaagcagct
      661 caagtttttg atgctcactt aatggttctt gctgacccag aaatgatcag ccaaatcaag
      721 gaaactatcc gtgcgaagaa agtgaatgca gaagcaggtc tgaaagaagt tacagatatg
      781 tttatcacta tctttgaagg catggaagac aacccataca tgcaagaacg cgcagcggat
      841 atccgcgacg tgacaaaacg tgtattggca aaccttcttg gtaaaaaatt gccaaaccca
      901 gcttctatca atgaagaagt gattgtgatt gcgcatgact tgactccttc agatacagct
      961 caattggaca aaaactttgt aaaagctttt gtaaccaaca ttggtggacg tacaagccac
     1021 tcagctatca tggcacgtac acttgaaatt gctgctgtat taggtacaaa caacatcact
     1081 gaaatcgtta aagacggtga catccttgct gttaacggga tcactggaga agtgattatc
     1141 aacccaacag atgaacaagc ggcagaattt aaagcagctg gtgaagccta tgcgaaacaa
     1201 aaagctgaat gggcactttt gaaagatgct caaacagtga ctgctgacgg taaacacttc
     1261 gagttggctg ccaatattgg tactccaaaa gacgttgaag gtgttaacaa caacggtgca
     1321 gaagctgttg gactttaccg tacagagttc ttgtacatgg attctcaaga cttcccaact
     1381 gaagatgagc agtatgaagc atacaaggct gttcttgaag gaatgaacgg taaacctgtt
     1441 gtcgttcgta caatggatat cggtggagat aaggaacttc cttacttcga tatgcctcac
     1501 gaaatgaacc cattccttgg attccgtgct cttcgtatct ctatctctga gactggagat
     1561 gctatgttcc gcacacaaat ccgtgctctt cttcgtgcgt ctgttcacgg tcaattgcgt
     1621 atcatgttcc caatggttgc gctcttgaaa gaattccgtg cagcgaaagc agtctttgat
     1681 gaagaaaaag cacaccttct tgctgaaggt gttgcagttg cggataacat tcaagttggt
     1741 atcatgatcg agattcctgc agcggctatg cttgcagacc aatttgctaa agaagttgac
     1801 ttcttctcaa ttggtacaaa tgacttgatc caatatacaa tggcagcaga ccgtatgaac
     1861 gaacaagttt cataccttta ccaaccatac aacccatcaa tcctacgctt gattaacaat
     1921 gtgatcaaag cagctcacgc tgaaggtaaa tgggctggta tgtgtggtga gatggctggt
     1981 gaccaacaag ctgttccact tcttgtcgga atgggcttgg atgagttctc tatgtcagca
     2041 acatctgtac ttcgtacacg cagcttgatg aagaaactcg acacagctaa gatggaagag
     2101 tacgcaaacc gtgcccttac agaatgctca acaatggaag aagttcttga acttcaaaaa
     2161 gaatacgtta attttgatta atcgaaaagt ccctgcaact cagttacagg gacttttttt
     2221 gatattttaa aaagaatttt caagaaaatc tttcttataa aaagtccaac cttgaaaaag
     2281 tagtggtcag aacaaaaaat acttaaatgg ttcataaaat tcttgacaag ttggagattt
     2341 aggagtaaac tattaaccag ttaagtaata gagaggagtt tctgcaattt agaaatgaat
     2401 tgcaactaga aatatcaaat agaaagagag tttcgatgaa aattaataag aaataccttg
     2461 ttggttctgc ggcagctttg attttaagtg tttgttctta cgagttggga ctgtatcaag
     2521 ctagaacggt taaggaaaat aatcgtgttt cctatataga tggaaaacaa gcgacgcaaa
     2581 aaacggagaa tttgactcct gatgaggtta gcaagcgtga aggaatcaat gctgagcaaa
     2641 tcgtcatcaa gataacagac caaggctatg tcacttcaca tggcgaccac tatcattatt
     2701 acaatggtaa ggttccttat gatgcgattt tcagtgaaga attactcatg aaagatccaa
     2761 actataagct aaaagatgag gatattgtta atgaggtcaa gggtggatat gttatcaagg
     2821 tagatggaaa atactatgtt taccttaagg atgctgccca cgcggataac gtccgtacaa
     2881 aagaggaaat caatcgacaa aaacaagaac atagtcaaca tcgtgaaggt gggactccaa
     2941 gaaacgatgg tgctgttgcc ttggcacgtt cgcaaggacg ctatactaca gatgatggtt
     3001 atatctttaa tgcttctgat atcatagagg atactggtga tgcttatatc gttcctcatg
     3061 gagatcatta ccattacatt cctaagaatg agttatcagc tagcgagttg gctgctgcag
     3121 aagccttcct atctggtcgg ggaaatctgt caaattcaag aacctatcgc cgacaaaata
     3181 gcgataacac ttcaagaaca aactgggtac cttctgtaag caatccagga actacaaata
     3241 ctaacacaag caacaacagc aacactaaca gtcaagcaag tcaaagtaat gacattgata
     3301 gtctcttgaa acagctctac aaactgcctt tgagtcaacg ccatgtagaa tctgatggcc
     3361 ttgttttcga cccagcgcaa atcacaagtc gaacagctag aggtgttgca gtgccacacg
     3421 gagatcatta ccacttcatc ccttactctc acatgtctga attggaagaa cgaatcgctc
     3481 gtattattcc ccttcgttat cgttcaaacc attgggtacc agattcaagg ccagaacaac
     3541 caagtccaca accgactccg gaacctagtc caggcccgca acctgcacca aatcttaaaa
     3601 tagactcaaa ttcttctttg gttagtcagc tggtacgaaa agttggggaa ggatatgtat
     3661 tcgaagaaaa gggcatctct cgttatgtct ttgcgaaaga tttaccatct gaaactgtta
     3721 aaaatcttga aagtaagtta tcaaaacaag agagtgtttc acacacttta actgctaaaa
     3781 aagaaaatgt tgctcctcgt gaccaagaat tttatgataa agcatataat ctgttaactg
     3841 aggctcataa agccttgttt gaaaataagg gtcgtaattc tgatttccaa gccttagaca
     3901 aattattaga acgcttgaat gatgaatcga ctaataaaga aaaattggta gatgatttat
     3961 tggcattcct agcaccaatt acccatccag agcgacttgg caagccaaat tctcaaattg
     4021 agtatactga agacgaagtt cgtattgctc aattagctga taagtataca acgtcagatg
     4081 gttacatttt tgatgaacat gatataatca gtgatgaagg agatgcatat gtaacgcctc
     4141 atatgggcca tagtcactgg attggaaaag atagcctttc tgataaggaa aaagttgcag
     4201 ctcaagccta tactaaagaa aaaggtatcc tacctccatc tccagacgca gatgttaaag
     4261 caaatccaac tggagatagt gcagcagcta tttacaatcg tgtgaaaggg gaaaaacgaa
     4321 ttccactcgt tcgacttcca tatatggttg agcatacagt tgaggttaaa aacggtaatt
     4381 tgattattcc tcataaggat cattaccata atattaaatt tgcttggttt gatgatcaca
     4441 catacaaagc tccaaatggc tataccttgg aagatttgtt tgcgacgatt aagtactacg
     4501 tagaacaccc tgacgaacgt ccacattcta atgatggatg gggcaatgcc agtgagcatg
     4561 tgttaggcaa gaaagaccac agtgaagatc caaataagaa cttcaaagcg gatgaagagc
     4621 cagtagagga aacacctgct gagccagaag tccctcaagt agagactgaa aaagtagaag
     4681 cccaactcaa agaagcagaa gttttgcttg cgaaagtaac ggattctagt ctgaaagcca
     4741 atgcaacaga aactctagct ggtttacgaa ataatttgac tcttcaaatt atggataaca
     4801 atagtatcat ggcagaagca gaaaaattac ttgcgttgtt aaaaggaagt aatccttcat
     4861 ctgtaagtaa ggaaaaaata aactaatgaa aaatgaaagt ctcgataaag aggcttt
//
LOCUS       DAVKJD010000098         4873 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKJD010000098 DAVKJD010000000
VERSION     DAVKJD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.098"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..761
                     /locus_tag="VW157_001827"
     CDS             <1..761
                     /locus_tag="VW157_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9553084.1"
                     /translation="KSELIPERVSQDIKFDKKIELKDIWYKYGLFDDYVLKGINVTIK
                     KGETVAIVGESGSGKSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLYRKIFGAVLQN
                     STLSYGTLRENLTFGHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIAEEGNNFSGGQ
                     QQMILLARCLLSKPSVVVLDEATSSLDNLSQQITTSYLSEIGTTKILIAHRLDTIKSA
                     DKILVMHNGEIVEIGTHRELLELGGIYKQLYSNN"
     gene            785..2523
                     /locus_tag="VW157_001828"
                     /pseudo
     CDS             785..2523
                     /locus_tag="VW157_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
     gene            2574..3341
                     /locus_tag="VW157_001829"
     CDS             2574..3341
                     /locus_tag="VW157_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEU9553085.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            3343..3975
                     /locus_tag="VW157_001830"
     CDS             3343..3975
                     /locus_tag="VW157_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9553086.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLNLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            4116..4394
                     /locus_tag="VW157_001831"
     CDS             4116..4394
                     /locus_tag="VW157_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HEU9553087.1"
                     /translation="MKKFDNYIIEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
BASE COUNT         1727 a          680 c          929 g         1537 t
ORIGIN      
        1 ctaaatccga attaattcca gaaagagtca gtcaagatat aaaatttgat aaaaaaatag
       61 aattaaaaga tatttggtat aaatatggat tatttgatga ttatgttttg aaaggaataa
      121 atgttactat taaaaaagga gaaactgttg ctattgttgg agaatcaggt tcaggtaaga
      181 gtacattagc taaaatttta ttaggtttat tagaacctaa tattggttca atagaagttg
      241 atggagtaga aaaagaagaa attggtcaaa cattgtatag aaagattttt ggagcagtgt
      301 tacaaaattc aaccctaagt tatggtacct taagagagaa tttgacattt ggacactttg
      361 tttcagatga agaattaatg acaaatctaa attcaattgg tcttagcaat gtagttaaat
      421 ctttacctct tggattagag acaatcatcg ctgaagaagg taataacttt tctggagggc
      481 agcagcaaat gatactttta gctcgttgtc ttttgtcgaa accttcggta gttgttttgg
      541 acgaagcaac aagtagttta gataatttat ctcaacaaat tacaacttct tacttaagtg
      601 aaatcggtac cactaagatt ttaattgccc atcgactaga tactatcaag tctgcagata
      661 agatcttagt aatgcataat ggtgaaattg tagagattgg gacccataga gaacttcttg
      721 aactaggagg catttataag caattgtatt caaataatta gtttttgatt aaaagggtaa
      781 atttatgaag attatgaaaa aaaaatattg gactttagcg atattattct tttgtttgtt
      841 caataattct gttactgctc aagaaatacc taaaaatctt gatggcaata taactcacac
      901 tcagactagc gaaagttttt ctgaatctga tgaaaaacag gttgactatt ctaataaaaa
      961 tcaagaagaa gtagaccaaa ataaatttcg tattcaaatc gataagacag aattatttgt
     1021 aacaacagat aaacatttag aaaaaaactg ttgtaaattg gaacttgaac cacaaataaa
     1081 taacgatatt gttaactctg aaagtaataa tttactaggc gaagataatt tagataataa
     1141 aattaaggaa aatgtttctc atctagataa tagaggagga aatatagagc atgacaaaga
     1201 taacttagaa tcgtcgattg taagaaaata tgaatgggat atagataaag ttactggtgg
     1261 aggcgaaagt tataaattat attctaaaag taattctaaa gtttcaattg ctattttaga
     1321 ttcaggagtc gatttacaaa atactggatt actgaaaaat ctttcaaatc actcaaaaaa
     1381 ctatgtcccc aataaaggat atttaggaaa agaggaggga gaggaaggaa taatatcaga
     1441 tattcaagat agattaggtc atggtacggc tgttgtagct caaattgtag gggatgacaa
     1501 tattaatgga gtaaatcctc acgttaatat taacgtctat agaatatttg gtaagtcgtc
     1561 agctagtcca tattggattg taaaagcaat ttttgatgct gtagatgatg gcaatgatat
     1621 tatcaatctt agtactggac aatatttaat gattgatgga gaatatgagg acggaacaaa
     1681 tgattttgaa acatttttga agtataaaaa ggctattgat tacgcgaatc aaaaaggagt
     1741 aattatagta gctgcattag ggaatgactc cctaaatgta tcaaatcagt cagatttatt
     1801 gaaacttatt agttcacgca aaaaagtaag aaaaccagga ttagtagttg atgttccaag
     1861 ttatttctca tctacaattt cggtcggagg catagatcgc ttaggtaatt tatcagattt
     1921 tagcaataaa ggggattctg atgcaatata tgcgcctgca ggctcaacat tatctctttc
     1981 agaattagga cttaataact ttattaatgc agaaaaatat aaagaagatt ggattttttc
     2041 ggcaacacta ggaggatata cgtatcttta tggaaactca tttgctgctc ctaaagtttc
     2101 tggtgcgatt gcaatgatta ttgataaata caaattaaaa gatcagccct ataattatat
     2161 gtttgtaaaa aaattctgga agaaacatta ccagtaaaaa atggtataaa agtgttaaat
     2221 ataccaaacg tattgagata tgatttgaat atgttacaat tagaatataa aaatgaacaa
     2281 agttgggata gtttcataga taatgttaat ttaattgagt tggaagagag aattcaaact
     2341 actattggaa ttaaacaaat aaacacacac aatattatta ctattgcccg agaagggtac
     2401 tctcaaaatt atttacctaa cacttcagaa aatacatata attcattaca agtcagttta
     2461 gttggagtat tactactttt tataagtatg gtaaatattt tatgggctaa aaaaagtaaa
     2521 tgaaaataaa atttggagcc ctctgaaaaa gtaagtccta cagttcaact aaaatgagtc
     2581 aaaagatgaa tcaccttgat gtaggggagt ttgtcttatt gctgcctgaa cacctccgtt
     2641 cagaggaaga acattataaa tctgtttttg aagacgactt aaccagtcgc atatctagtc
     2701 aagatgaacg acagcaaatg actgctacgg taggttattt agaatcaggt caggatcgtt
     2761 ttgtgtataa tacgacccct atttcttacc agcagttttt gaaagatcca atcatcattg
     2821 ttataacacc ccaatcaact ggtccacagt ccattttgtt ttggatagac gcagtacaga
     2881 actacgttct ctttaatcaa ttgtctgatg cccaggagct tatccagaga caaggcattg
     2941 aaaattgggt ctcagaaatg caaacaggtt accacaacta catcacatta ttggataata
     3001 tccagaggga acgttgggta atgctagcag gagctgtgct tgggattgca acttcaatct
     3061 tgttgtttaa cactatgaat aggctctact ttgaagaatt tagacgtgcc atttttatca
     3121 aacgcattgc aggtctcagg ttcttagaaa tccatcgcac ttatctcttt gctcaactgg
     3181 gtgtgttttt actgggattt gttgcgagtg tatttcttca ggtagagata ggagttgctt
     3241 tcttagtctt gttactcttt actggtctat ctcttttaca gttacatgtc caaatgcaga
     3301 aagaaaacaa gatgtccatt cttgttttga agggaggtta atatgattga acttaaacag
     3361 gtgagtaaat cttttggaga acgagagtta ttttcgaatc tttcaatgac atttgaggct
     3421 ggaaaagtct atgccttaat tggttcaagt ggtagcggaa aaacaacctt gatgaacatg
     3481 attgggaaat tagaacctta tgatgggacg attttttacc gaggtaaaga cttggccaat
     3541 tataaatcga gtgatttttt ccgtcacgaa ttgggctacc tcttccagaa ctttggctta
     3601 attgaaaacc aaagtattga agaaaacctt aagctaggtc tcattggtca aaagttgagt
     3661 cggtcggaac agcggttgag gcagaagcag gctttagaac aggtcggcct ggcttatctt
     3721 aacctagata agcgcatctt tgagttatcg ggcggagaat cgcaacgggt tgccttggca
     3781 aaagttatct taaagaatcc accctttatt ctggcagatg agccaacagc ttcaatagac
     3841 ccagcaactt ctcagttgat tatggagatt ttgctatctc ttcgagatga taataggcta
     3901 atcattatcg caacacataa tccggcaatt tgggagatgg ctgatgaagt gttcacgatg
     3961 gatcgtctga aataaaaatc cttgttttta attgcacgat gagttactga aatattatca
     4021 tgaatcaaga attggagtta atttagaatt gtactttatt aatattgagg taactttttc
     4081 ttgataaagg aagaaatagt agagaggaag ttagaatgaa aaaattcgac aattatatta
     4141 ttgagaagcc ttgcgattct aattcagata aactgcaaaa aatcttaata attgaaaatt
     4201 tggtagatga tattttgcaa tttcctctca gaatcaataa tagtgtagga gagattttcc
     4261 tcctacaacc gtttcaaaag aaaactatct ttattccatg ttattttgag gaagatattg
     4321 tgaaagtcaa agatgatgat aaagttgagt ggaatttgtt agaatttcaa aaatttagag
     4381 catttttggc ttagtaatct gtgttgaagg ctcaaaacct atggtaaaaa agtcgctttg
     4441 aaaacgtatt gcctccaaag atttagttaa ataatgattt aacacaaaaa gaaattattg
     4501 aagttctgga aagatgttgt ttcagtattg agaaaaggtg ggaaaaactt gcgattttca
     4561 cagagaaagg aagaaaaagt atagaaatat agtcaattga aacaagaaca ggataaaaga
     4621 accttttgtg ccatattttt ctcctttcgc tttacaattg gattgaacac ctttattgta
     4681 tcgcgtttgg agtttttttg gtataacctt cgacgcgcac ccgcatagcg ggtgtttttt
     4741 ttgtctcgca cctaacggag cgagacaaac taatagtcac ttaatcaaaa aacgcaccat
     4801 atcaaaaact aaaaagtttg atatcatgcg tcatgtctta aactaattga ctatactttc
     4861 tattcaaatg agc
//
LOCUS       DAVKJD010000099         4741 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKJD010000099 DAVKJD010000000
VERSION     DAVKJD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.099"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            40..255
                     /locus_tag="VW157_001832"
     CDS             40..255
                     /locus_tag="VW157_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553088.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(364..1104)
                     /locus_tag="VW157_001833"
     CDS             complement(364..1104)
                     /locus_tag="VW157_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9553089.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(1104..2669)
                     /locus_tag="VW157_001834"
     CDS             complement(1104..2669)
                     /locus_tag="VW157_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9553090.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKVIYS
                     LQKLVGWILNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMCKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            2807..4696
                     /locus_tag="VW157_001835"
     CDS             2807..4696
                     /locus_tag="VW157_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9553091.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
BASE COUNT         1315 a          866 c         1100 g         1460 t
ORIGIN      
        1 tacaattaaa tattgtaaag aagaaaatag gagaaaaaca tggctaagtc aaactttgaa
       61 aaagtagaat cagttgttgg ctgggttcgt gataagaaaa tcacaggcta ccgtatctct
      121 aaagaaacga atgcgcgtga aatgtctatc attgctctgg cgcagggtcg tgcaaaagta
      181 aaaaatattt catttgaaac agccctaggc ctaattgatt tctatgaaaa aaattatgaa
      241 aaatttgaag attaatcttt ggataacggc ggattcttga ccttcaagta gtagagatag
      301 agaatctgcc ttttcatttt gaggacagca aaaagactgc acggttgatg cagccttttc
      361 tttttatttg agatagcgtt gaaggaactc ttttgttcgg tcttctttag gattggtgaa
      421 gaggtcttct ggtttacctt cttcagcgat cacgccctta tccataaaga taacacggtg
      481 agagacatca cgggcaaatt ccatttcatg ggttacgaca atcatggtca agccttcctg
      541 agccaggtcc tgcatgattt tgaggacttc tccaaccatt tctggatcga gagctgatgt
      601 tggttcatca aagagaatag cgtccggatt catggagagg gcacgagcga tggccacacg
      661 ttgtttttga ccacctgaga gttgttttgg tttggcttgc cagtagcgtt ctcccatgcc
      721 gaccttttcc aggttttctt tggcaatctt ttcagcttct gtgcgttcgc gttttaggac
      781 agttgtctga gcgacgattg tgttttcaag aacattgaga ttttcaaaga ggttaaagga
      841 ttggaaaacc atccccaact tttcacggta ttgcgtgagg tcatagcctt tttcgaggac
      901 gttttgtcca tgataaagga tttgtccatc agttggtgtt tcaagtaggt taatggagcg
      961 taggaaggtc gattttccgc ttccagagct tccgatgata gagatgacct ctcccttgtg
     1021 gacagtgagt gaaatgtctt ttagcacttc gttttgtcca taggattttt tgaggtgttt
     1081 aatttcaagg attgcttgtg tcattatttc aaatcctccg tttgcatttg gttagcacct
     1141 gtagtgtagg tatccatgtc cattctgcgc tcgataaagc gtaggatacg tgttacggtg
     1201 aaggtgagga caaagtaaat cacggcgatg attgtaaatg tctggaagta ttgataggtt
     1261 tgtgttgcca cggtatttcc tgagaaataa agctcgacaa cagatataac gttcaataca
     1321 gatgtatctt tgatattgat gacaaattca ttaccagttg ctggtaggat attgcggaca
     1381 acctgaggta ggacaatctt acacatggtt tggttatgag tcataccaag agcagtcgca
     1441 gcttcaaatt gtcccttgtc aactgccagg ataccaccac ggacgatttc agtcatgtag
     1501 gcaccggtat tgattgaaac gatgaagata gcagccagtg tacggtcaag gttgataccg
     1561 aaagcttggg cagttccata gtagataacc atcgattgaa caatcattgg cgtaccacgg
     1621 aaaatttcaa tgtagacgtt gaggatccag ccgactagtt tttgcaggct gtaaatgact
     1681 ttgttttcag agagaggagc agtacggaag acaccaatgg caagtccaat aatgagacct
     1741 atgatggttc cgacgataga gattaaaaga gtgataccag caccacgcaa gagttgttgc
     1801 cagttttcag aaagaatttt agcgacttgg ctaaagaaac tactgctagt ctcttcagtt
     1861 gttgtagctt cggcaggttg ttccttgatc atacgatcca tcaaggcaac ttggtcatct
     1921 tttgaaatgg tttcaatgct ggcattgatt tggctaatac gattgtcatt tttacgaagc
     1981 ccgatagcga tagctgtatc ttcttcccca gttttgaaac caggttctac ttgaatcatc
     2041 ttgaacttag agttcgcagc ttcagcagtc agtgcttctg gacgttcaga aacataagca
     2101 tcaatgacac cagcctcaag agcttggcgc atttgagcga agtctcccat ggctgtttct
     2161 tttttagcac ctgggatttg tgcaatcaag tcgtagaggt agacaccttg ttgagaagtg
     2221 attttcgcac cattaaagtc atccaaagat ttagcacttg cgtaggcaga atcttttttg
     2281 acaagcaaaa ctggttcgct agtgtagtaa ctgctcgaaa aggcaatttc ttgtttgcgt
     2341 tctgcagttg gactcatacc tgcgataatc atgtcaatct taccagaagt aagggcaggg
     2401 actagacctt cccacttggt tttaacaacc aaaggttctt tacctaagtc cttagcgatt
     2461 ttcttggcga tttgaacatc gtatccgttg gcatactgat tggtcccatc gattttgaca
     2521 gctccgttgc tatcatcatc ctgggtccag ttaaagggag catatgctgc ttccatcccg
     2581 atgcgtaaat attcatcggc ttgagcaaca ttgacaagtc ctagcatcag caagagactt
     2641 gtgaaaatag ataggtatat ttttctcatg atttctccta ttctgatcta ttaaaaaata
     2701 actgtctcct attttatcga aaaatgcgta atttttcaac ataagtaagc ctttacttac
     2761 gaaaagatgc tataatgata agaaagataa aaagggggct tagttgatga aaaaaacttt
     2821 tttcttactg gtgttaggct tgttttgcct tcttccactc tctgtttttg ccattgattt
     2881 caagataaac tcttatcaag gggatttgta tattcatgca gataatacgg cagagtttag
     2941 acagaagata gtttaccagt ttgaggagga ctttaagggc caaatcgtgg gacttggacg
     3001 tgctggtaag atgcctagcg ggtttgacat tgaccctcat ccaaaggttc aggctgcgaa
     3061 aaacggtgca gaactagcag atgtgactag cgaagtaaca gaagaagcgg atggttatac
     3121 tgtgagagtc tataatccag gtcaggaggg cgacatagtt gaagttgacc tcgtctggaa
     3181 cttaaaaaat ttacttttcc tttatgatga tatcgctgaa ttaaattggc aacctctgac
     3241 agatagttca gagtctattg aaaagtttga atttcatgta aggggagaca agggggctga
     3301 aaaactcttt ttccatacag ggaaactttt tagagaggga acgattgaaa agagtaacct
     3361 tgattatact atccgtttag acaatcttcc ggctaagcgt ggagttgagt tgcatgccta
     3421 ttggtctcgg accgattttg ctagcgctag ggatcagggc ttgaaaggga atcgtttaga
     3481 agagtttaat aagatagaag actcgattgt tagagaaaaa gatcagagta aacaactcgt
     3541 tacttgggtc ctcccttcga tactttctat ctccttgtta ttgagtgtct gcttctattt
     3601 tatttataga agaaagacca ctccttcagt caaatatgcc aaaaatcatc gtctctatga
     3661 accaccaatg gaattagagc ctatggtttt atcagaagca gtctactcga cctccttgga
     3721 ggaagtgagt cccttggtca agggggctgg caaattcact tttgaccaac ttattcaagc
     3781 taccttgcta gatgtgatag accgtgggaa tgtctctatt atttcagaag gagatgcagt
     3841 tggcttgaga ttggtgaaag aagatggttt gtcaagattt gagagagact gtctaaatct
     3901 agccttttca ggcaaaaaag aagccactct ttccaatttg tttgcggatt acaaggtatc
     3961 tgatagtctt tatcgtagag ccaaagtttc tgatgaaaaa cggattcaag caagagggct
     4021 tcaactcaaa tcttcttttg aagaggtatt gaaccagatg caagaaggag tgagaaaacg
     4081 agtttccttc tgggggctcc cagattacta tcgtccttta actggtttgg aaaagacttt
     4141 gcaagtgggt atgggtgtct tgactatctt gcccctattt atcggatttg gtttgttctt
     4201 gtacagttta gacgttcatg gctatcttta cctccctttg ccaatacttg gttttctagg
     4261 gttagttttg tctgttttct attattggaa gcttcgacta gataatcgtg atggtgttct
     4321 aaatgaagcg ggagctgagg tctactatct ctggaccagt tttgaaaata tgttacgtga
     4381 gattgcacga ctggataagg ctgaattgga aagtattgtt gtttggaatc gtctcttggt
     4441 ctatgcaacc ttatttggct atgcggacaa ggttagtcat ttgatgaagg ttcatcagat
     4501 tcaagttgaa aatccagata tcaatctcta tgtagcttat ggctggcaca gtatgtttta
     4561 tcattcaagc gcgcaaatga gccattatgc tagtgtcgca aatacagcaa gtacctactc
     4621 cgtatcttct ggaagtggaa gttctggcgg tggcttctct ggaggcggag gtggcggcag
     4681 tatcggagcc ttttaaagag agctaccata gactgaaaaa gtatgatata atggaagata
     4741 g
//
LOCUS       DAVKJD010000100         4695 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKJD010000100 DAVKJD010000000
VERSION     DAVKJD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.100"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            4..708
                     /locus_tag="VW157_001836"
     CDS             4..708
                     /locus_tag="VW157_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9553092.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     misc_feature    767..854
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            890..1204
                     /gene="rplU"
                     /locus_tag="VW157_001837"
     CDS             890..1204
                     /gene="rplU"
                     /locus_tag="VW157_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9553093.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            1220..1564
                     /locus_tag="VW157_001838"
     CDS             1220..1564
                     /locus_tag="VW157_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9553094.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            1581..1874
                     /gene="rpmA"
                     /locus_tag="VW157_001839"
     CDS             1581..1874
                     /gene="rpmA"
                     /locus_tag="VW157_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9553095.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(2185..2390)
                     /locus_tag="VW157_001840"
                     /pseudo
     CDS             complement(2185..2390)
                     /locus_tag="VW157_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            2828..3745
                     /locus_tag="VW157_001841"
     CDS             2828..3745
                     /locus_tag="VW157_001841"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9553096.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPIEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            complement(3838..4686)
                     /locus_tag="VW157_001842"
     CDS             complement(3838..4686)
                     /locus_tag="VW157_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9553097.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
BASE COUNT         1483 a          814 c          958 g         1440 t
ORIGIN      
        1 acaatgggag gtaaaatgag gttattacct ataagaaaaa tatcacgtca gtctaaaagg
       61 ttagcacttt ttttgacgtt ttgtgctgga tatgtggatg cttacacttt tattgttcgc
      121 gggaataccc ttgtagctgg acaaactgga aatgttgtct ttctttcagt agaattaatt
      181 aaaaataatg tttcggatgt tagggacaag gttctcacct tgctagcgtt tatgatggga
      241 gtctttttat taacgattta taaggaaaaa ttgagaattg tgaaaaaacc tattctgtcc
      301 ttgattccct tggcaatctt atcaatcatt attgcttttg tgccgcaaac tgtggataat
      361 atctatctag tgccgccctt ggccttctgt atgggactgg tgacaactgc ttttggtgag
      421 gtttcgggta ttgcctacaa taacgctttt atgacaggga atatcaaacg gaccatgctg
      481 gcttttggag attatttccg aaccaagcac actccttttt tgcgtgaagg attcatattt
      541 gttagcctgc ttagtagttt tgtccttggc gttgtctttt cagcctattt gacgattttc
      601 tatcatgaaa agaccattct tggtgttcct attatgatga gcgtttttta cctcagcatg
      661 ctttttgcct cttggcagaa aaaagtaaaa gaaaaagctt cattttaggt gttattactt
      721 gtaagaaaat aggagatatg ctatacttga agagttgact atgcacgatc ctgtgcaacc
      781 gcacgaacat cgttgcttga ttattcaagt ttaagtggtt cgtcccacga tgctaggcga
      841 gtcttcacaa aaaatcgggg aaacccataa aaatcatagg aggtgcataa tgagcacata
      901 cgcaattatc aaaactggcg gaaaacaagt taaagttgaa gttggtcaag cagtttacgt
      961 tgaaaaattg aacgttgaag ctggtcaaga agttactttt aacgaagttg ttcttgttgg
     1021 tggtgaaaac actgttgtcg gaactccact tgttgctgga gctactgtag ttggaactgt
     1081 tgaaaaacaa ggaaaacaaa agaaagtggt tacttacaag tacaaaccta aaaaaggtag
     1141 ccaccgtaaa caaggtcacc gtcaaccata tacaaaagtt gtcatcaacg caatcaacgc
     1201 ttaattttaa ggagaacaca tgatacaggc agtctttgag agagccgaag atggcgagct
     1261 gaggagtgcg gaaattactg gacacgccga gagtggcgaa tacggcttag atgtcgtgtg
     1321 tgcatcggtt tctacgcttg ccattaactt tatcaattct attgagaaat ttgcaggcta
     1381 tgaaccaatc ctagaattaa acgaagatga aggtggctat ctgatggttg aaataccaaa
     1441 agatcttcct tcacaccaga gagaaatgac ccagttattc tttgaatcat ttttcttagg
     1501 tatggcaaac ttatcggaga actcttctga gttcgtccaa accagagtta tcacagaaaa
     1561 ctaacacgga ggaaaacatt atgttaaaaa tgactcttaa caacttgcaa cttttcgccc
     1621 acaaaaaagg tggaggttct acatcaaacg gacgtgattc acaagcaaaa cgtcttggag
     1681 ctaaagcagc tgacggacaa actgtaacag gtggatcaat cctttaccgt caacgtggta
     1741 cacacatcta tccaggtgta aacgttggtc gtggtggaga cgatactttg ttcgctaaag
     1801 ttgaaggcgt agtacgcttt gaacgtaaag gacgcgataa aaaacaagtt tctgtttacc
     1861 caatcgcaaa ataaaaaggt tcattgaacc ttttatcccg aaccttgaaa tgtaaaggtg
     1921 aggaagctag aaacagctta aaataaggct ttccgagttt tcggagagcc tgttttttta
     1981 ttttgatacc catacttttt aatatgtata gtggattgat actcttcgaa aatcaaattc
     2041 aaaccacgtc aatttcacct tgccgtactc aagtacagcc tgcggctaac ttcctagttt
     2101 gcactttaat tttcattgag tatgaatctg gaatagaaca ctgttaaaaa ttgatatgag
     2161 tttcctactc gatttcttca gattttattt caattgacta taagaactgc tatctggttt
     2221 caaatattgg cttgtcgtcc cttcacttgt ctgaatggtt tgagccgttc cttctgataa
     2281 gggaaaagat attctttagc tgaaactgtc tgagaatcca agctaacaga tggagagaaa
     2341 aatagcccag tctgaagcac actagatggg agttgttgga aagcagtcat ctagtttttc
     2401 tttgctgttt tggctttttt ctttgtcatc agggaaagtg acttgcttca aatcagggag
     2461 tttaggaaat tcttcaaaca tcttgcccaa gttgtctcat tttgtaaatt ttacagccat
     2521 aggtgtacct cgattctaaa ttctaccttt tcggcaccaa ttagtaattt aaagttctca
     2581 ggagttagtt gcattgcttt ttcatacata agcatagtat gacactttat ggttaccgaa
     2641 caagtctaat cactccttgc aaaattttga agatgtccta caagtttttg agaagaatat
     2701 tgcaaagttc atagttaagc aacgatagaa taaagtgctt tttgaaaaca ggtacacttc
     2761 atgtgaaaaa aacaacattt cagattttct gttccagaaa tagctatcgc tatgatataa
     2821 ttattttatg attattacta ttcctataaa aaaccaaaaa gatattggca caccatctga
     2881 ttcagtcgtt gttctcggct attttgatgg catacataag gggcatcaag aattatttcg
     2941 tgttgccaac aaggctgcga gaaaggattt attacctatc gtcgttatga cctttaatga
     3001 atctccaaag atcgctttag agccttatca tccagatttg tttttgcata ttttgaaccc
     3061 tgctgaacgt gaaagaaaat taaagcgcga aggtgtagaa gaattatatc tccttgattt
     3121 tagtagtcaa ttcgctagtc tcactgcaca agaattcttt gcaacttata tcaaggctat
     3181 gaatgccaaa attattgttg caggttttga ttatacattt ggttctgaca aaaaaacagc
     3241 agaagattta aaggattact ttgatggaga agttatcatt gttccaccta tagaagatga
     3301 gaaaggaaag attagttcaa ctcgtatccg tcaagctatt ttagatggaa atgtgaaaga
     3361 agcaggaaaa cttttggggg caccgcttcc atcaagaggt atggtggttc atggtaatgc
     3421 tcgtggtcgt acaattggtt atccgacagc gaatttagtg cttttagacc gtacttatat
     3481 gccagcagat ggcgtttatg tcgttgatgt tgagattcaa agacagaagt atcgtgctat
     3541 ggctagtgtc gggaaaaatg tgacctttga tggagaagaa gcacgttttg aagtcaatat
     3601 ttttgatttt aatcaagata tttatgggga aaccgtcatg gtttattggc ttgatcgcat
     3661 tcgtgatatg accaaatttg actcagttga ccaattagtg gatcagttaa aggctgatga
     3721 agaagtaact cggaattggt cttaagagct tgagtaaata aaataaaaaa gaggttgtct
     3781 gtaacccaaa agatagatga tttagtctaa cttttgaggt cacgacatta cctcttttta
     3841 ttctttttca aaggtgaagc cttctcctag gatttcatgg gcttctgtaa tagttataaa
     3901 ggcttgagga tcgattcgat gaatcatttc cttcgttttc acaatttcat ttcttccgac
     3961 aatacagtag atgattttca aattttcttt actatagtag ccttgaccag aaataaaagt
     4021 aacacctctt ccgaggtcat cattaatcgc cttagcaagt tggtcaggac gttttgtgat
     4081 aatcataaag cctttgccgg catatcctcc ttcaccaatc aaatcaataa cacgagaaac
     4141 aataaaatca aacaaaagcg tgtaggaaac caatctcaaa tccttgaaga ttaggagaat
     4201 caacatgaga atacaaaaat ctaagataaa gagcagtttt cctatggata tatgagtgta
     4261 tttgttgaga atacgagcta gaatatcagt tccgccagtt gtacctccag cattaaaaat
     4321 aattccaagg ccaattccca ataggattcc cgctataagg gctgtgatta gtaaatcacc
     4381 ttgaagatca atatgaaggg gaatatgctc aaaaaaagct aaccagccgg acaaagctaa
     4441 ggttcctagt aaactagaat agagggattt ggctccaaaa atcttccaag ctaggatgaa
     4501 aaggggaata ttaatcagca ggttcatgag ggaaacaggg attttaaaaa gataaaaggt
     4561 gatgagggta atgcctgtcg cccctccttc aaagagatga tggggaacta caaaataagt
     4621 cagtccaaaa gcataaatag cagcacctag taaaatggta aaaatggggt aaattttttt
     4681 aatcatagga cacct
//
LOCUS       DAVKJD010000101         4595 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKJD010000101 DAVKJD010000000
VERSION     DAVKJD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.101"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(121..1107)
                     /locus_tag="VW157_001843"
     CDS             complement(121..1107)
                     /locus_tag="VW157_001843"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9553098.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            1301..3769
                     /gene="gyrA"
                     /locus_tag="VW157_001844"
     CDS             1301..3769
                     /gene="gyrA"
                     /locus_tag="VW157_001844"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9553099.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLVITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            3769..4515
                     /gene="srtA"
                     /locus_tag="VW157_001845"
     CDS             3769..4515
                     /gene="srtA"
                     /locus_tag="VW157_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9553100.1"
                     /translation="MSQKKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIV
                     WNTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIP
                     ELEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDN
                     AKNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERII
                     VKGNLKETKDYSQTSNEILTAFNQPYKQFY"
BASE COUNT         1414 a          810 c         1024 g         1347 t
ORIGIN      
        1 cattttataa caacgtttta gagtaatggg gtgctatttc aacttcaacc tactataata
       61 cagaaaaaaa tacagaaaaa aacaactccc tgatgattca aggagttgtc tatagttaaa
      121 ttagttttta gaagcttctt ggaattctgg atttttccat gcttcgtcaa taatagcttg
      181 caattcttta gcagatgctt gcattttttg agtttctgca tcgttcaatg gaatgtttac
      241 tggacgaacg ataccatgtg caccaacaac agctggttga ccgataaaga cattctcaac
      301 tccgtattga ccttcttgga atactgaaag tggaagtact gcgttttcat cgtcaaggat
      361 tgctttagtg atacgagcaa gggctactgc gataccgtag tatgttgcac cttttttgtt
      421 gatgattgtg taggctgcat cacgaacacc ttcgaacaat tcaatcaatt cagcttcttg
      481 aacattttga gtgtctttaa ggaattcttc aaggtttaca ccagcgatgt tagcgtgtga
      541 ccaaacagcg aactcagagt caccgtgttc acccatgatg taggcgtgca ctgaacgagc
      601 atccacatcc aatttttcag caagtgcttg acggaaacga gctgagtcaa gtgaagtacc
      661 tgaaccgata acgcgttctt tagggaaacc agagaatttc caagttgagt aagtcaaaac
      721 gtcaactggg ttagcagcaa caaggaagat acctttgaaa ccagattcaa caacttgagt
      781 tacgattgat ttgttgatag caaggttttt acctacaagg tcaagacgag tttcacctgg
      841 tttttgaggt gcacctgcag tgatcacaac aaggtcagcg tctgcacagt cagagtattg
      901 agctgcatag atttttttag gtgaagtgaa ggcaagggcg tgactaaggt caagcgcatc
      961 accaacagct ttttcatgca attgtggaat ttcgataatt ccaagctctt gtgcaattcc
     1021 ttggttaaca agtgcaaaag cgtaagatga acctacagca ccatcaccga caaggataac
     1081 ttttttgtgt tgtttagttg aagtcattgt tttaaacatc tccttaattt tattagggga
     1141 ttttccctag acaacttcat tctatcactt ttaaaaaact ttgtcacgaa tatgccttat
     1201 agttctcgat gtaaacgttt tagtggttta gaggctgaaa tagatgggaa tttatggtat
     1261 aatgttgtta cttactaatt gtgaaatgag gcatttatta atgcaggata aaaatttagt
     1321 gaatgtcaat ctgacaaagg agatgaaggc aagttttatc gactacgcca tgagtgttat
     1381 cgtagcgcga gctcttcctg atgttcgaga tggcttaaaa cctgttcacc gtcgcattct
     1441 ctacggaatg aatgaattgg gtgtgacccc agacaaaccc cataaaaaat ctgctcgtat
     1501 tacaggggat gtcatgggta aatatcaccc acacggggat tcctctattt atgaagccat
     1561 ggtccgtatg gctcaatggt ggagctaccg ttacatgctt gtagatggtc atgggaattt
     1621 tggttccatg gatggagata gtgctgccgc tcaacgttat accgaggcac gtatgagcaa
     1681 gattgctctg gaaatgcttc gtgatatcaa caaaaataca gttgatttcg ttgataacta
     1741 tgatgccaat gaacgggaac ccttggtctt gccagcgcgt tttccaaacc ttttggttaa
     1801 tggagcaact ggtatcgcgg ttgggatggc aaccaatatt ccacctcata atctgggtga
     1861 aaccattgat gcagtgaagt tggtcatgga taatcctgaa gtgactacca aggacttgat
     1921 ggaagtcttg cctggaccag attttccaac tggtgctctt gtcatgggga aatcaggtat
     1981 ccataaggct tatgaaacag gtaaaggttc gattgtccta cgttctcgta cagagattga
     2041 aacgactaag actggtcgtg agcgcatcgt tgtaacagaa tttccttaca tggtcaataa
     2101 aaccaaggtg catgagcata ttgttcgctt ggttcaggaa aaacgcattg agggtatcac
     2161 agcagtacgt gatgagtcaa accgtgaagg tgttcgattt gttattgaag tcaagcgcga
     2221 cgcctcagcc aatgttattc tcaataacct cttcaaaatg acccaaatgc aaaccaattt
     2281 tggtttcaat atgctcgcta tccaaaatgg tataccgaaa attttgtctc ttcgtcagat
     2341 tttggatgct tatatcgagc accaaaaaga agtggttgtt cgtcgtacac gttttgataa
     2401 ggaaaaagcg gaagcgcgcg ctcatatctt agaaggtctc ttgattgcgc tagaccatat
     2461 cgacgaagtg attcgtatca tccgtgctag tgaaacggat gcggaagctc aagctgagtt
     2521 gatgagcaag tttaagcttt ctgaacgtca aagtcaagct atccttgata tgcgtcttcg
     2581 tcgtttgaca ggtttggaac gcgataagat tcaatctgag tatgatgacc tcttggctct
     2641 gattgcggat ttagcagata ttcttgctaa gcctgaacgt gtttctcaaa ttatcaaaga
     2701 cgaattggat gaagttaaac gtaaattttc tgataaacgc cgtacagagt tgatggttgg
     2761 acaggtcttg agtctcgagg atgaggactt gattgaagaa tcggatgtct tgattaccct
     2821 ttctaacaga ggctacatta agcgtttgga tcaggacgag ttcactgctc aaaaacgtgg
     2881 gggtcgtggt gtccaaggaa cgggagtgaa agatgatgac tttgttcgtg agttagtgtc
     2941 aactagcacc catgatcatc tgctcttctt cacaaacaag ggacgtgtct atcgtcttaa
     3001 aggttatgaa attcctgagt atggtcggac tgccaaaggg ctaccagtag tcaatctctt
     3061 gaaattggat gaagacgaaa gtattcagac ggttatcaat gttgagtctg atcgcagtga
     3121 tgatgcttat ctcttcttta caacccgtca cggtattgtg aagagaacca gtgttaagga
     3181 gtttgccaat attcgtcaaa atggtctcaa agcgctgaat ttaaaggatg aagatgagtt
     3241 aatcaatgtc ttgttggcag aaggagatat ggatattatc attggtacca agtttggtta
     3301 tgcagttcgc tttaatcaat cagccgttcg tggtatgagc cgtatcgcca ctggtgtgaa
     3361 aggtgttaac cttcgtgaag gagacacagt tgttggtgcc agcttgatta ctgatcaaga
     3421 tgaggttctt gttatcacag aaaaaggata tggtaagcgt acagtcgcta ctgaataccc
     3481 aacaaaaggt cgtggtggta agggaatgca gacagctaaa attaccgaaa aaaatggctt
     3541 gctggccggt cttatgactg ttcaagggga tgaggatttg atgattatca ctgatacagg
     3601 tgtcatgatt cgaaccaatc ttgccaatat ttcacaaaca ggacgtgcaa ctatgggagt
     3661 taaagtaatg cgcctggatc aagatgctca gatagtgact ttcacaacgg ttgcggtggc
     3721 agaaaaagaa gaagttggga cagaaaacga aacagaaggt gaagcataat gtctcaaaaa
     3781 aaaaataata aaaagaaaaa caagcgaaaa aatttgctga caaatatcct agcaggattt
     3841 ctgatattac tgtcactggc tttgattttt aatactcaaa ttcgaaatat tttcatagtc
     3901 tggaatacca ataagtatca agttagccag gtatcaaaag aaaaattaga agaaaatcag
     3961 gatacagaag gcaattttga ctttgattct gtcaaagcta tctcttcgga agctgttcta
     4021 acttctcaat gggatgctca aaaattacca gttattgggg gaattgcaat tcctgaattg
     4081 gaaatgaatt tgccgatttt taaaggactt gataatgtta atctcttcta cggagctggt
     4141 acaatgaaac gcgagcaagt aatgggagaa ggaaattata gtctagctag tcaccatatc
     4201 tttggtgttg ataatgctaa taaaatgtta ttttctcctt tagataatgc taaaaatggc
     4261 atgaagattt atctaaccga taaaaataaa gtttatactt atgaaatacg tgaagtcaaa
     4321 cgtgtgacac cggatcgtgt tgatgaagtt gatgatagag atggggtcaa tgaaatcaca
     4381 ttagtaacct gtgaagacct tgctgctaca gaacgtatta ttgtcaaagg taatttgaaa
     4441 gaaacaaaag attattcaca aacatctaat gaaatcctaa cagctttcaa tcaaccatat
     4501 aaacaatttt attaatacaa atcagtgaaa tcatggattt cactgatttt ttaagatttt
     4561 cataaaaata tagtaaaatg aaataagaat aggac
//
LOCUS       DAVKJD010000102         4536 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKJD010000102 DAVKJD010000000
VERSION     DAVKJD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.102"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(144..215)
                     /locus_tag="VW157_001846"
     tRNA            complement(144..215)
                     /locus_tag="VW157_001846"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Arg,seq:cct)
     gene            complement(258..605)
                     /gene="rplS"
                     /locus_tag="VW157_001847"
     CDS             complement(258..605)
                     /gene="rplS"
                     /locus_tag="VW157_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9553101.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(724..1053)
                     /gene="crcB"
                     /locus_tag="VW157_001848"
     CDS             complement(724..1053)
                     /gene="crcB"
                     /locus_tag="VW157_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9553102.1"
                     /translation="MVIVYLAIACGFGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLILTYLGGLVA
                     IFLGILL"
     gene            complement(1047..1421)
                     /gene="crcB"
                     /locus_tag="VW157_001849"
     CDS             complement(1047..1421)
                     /gene="crcB"
                     /locus_tag="VW157_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9553103.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(1418..1684)
                     /locus_tag="VW157_001850"
     CDS             complement(1418..1684)
                     /locus_tag="VW157_001850"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9553104.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(1800..2243)
                     /locus_tag="VW157_001851"
     CDS             complement(1800..2243)
                     /locus_tag="VW157_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9553105.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2347..3282
                     /locus_tag="VW157_001852"
     CDS             2347..3282
                     /locus_tag="VW157_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9553106.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3336..3620
                     /locus_tag="VW157_001853"
     CDS             3336..3620
                     /locus_tag="VW157_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9553107.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3677..3895)
                     /locus_tag="VW157_001854"
     CDS             complement(3677..3895)
                     /locus_tag="VW157_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553108.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRYNS
                     IAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(3892..4466)
                     /locus_tag="VW157_001855"
                     /pseudo
     CDS             complement(3892..4466)
                     /locus_tag="VW157_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1414 a          994 c          808 g         1320 t
ORIGIN      
        1 atatagtctc aattttatct gatgattgcc caaatttttc aataataaaa cgcataatat
       61 tatggagaca cacaaaatca cctatattat gcgttctttt aatatcaaag actttttgac
      121 aaacttcttt gatgtctaat tactgccccc tgcaggaatc gaacctgcaa ctactcctta
      181 ggagggagtt gttatatcca ttgaactaag ggagctagat aaaaactctg ctaaatgagc
      241 agagtttttt agtcgaatta acgacggatt tctttgatac gagctgcttt accttgaaga
      301 gcacgcaagt agtacaattt cgcacgacgt actttaccgt aacgaacaac ttcgattttt
      361 tcaacacgtg gagtgtggat tgggaagata cgctcaacac ctacaccgtt agagatttta
      421 cgaactgtgt agttttctga gattccagca cctttacgtg cgataacaac accttcaaaa
      481 atctggatac gttcacggtt accttcgaca actttcgcgt gtacacgaac agtatcacca
      541 ggacggaatg atgggatatc tgtacgaagt tgaccttcag tcaagctttg gattaatgga
      601 ttcattttat tctcctatct ttgtcaatct tgaggaatct tcctcagcgg ataaactgta
      661 tttttgtgcg tccattacac acaagatata gtttaccaaa tttctaaaaa aaagtaaaga
      721 aatttatagc aaaattccta aaaaaatcgc aaccaaacca cctaggtagg tcaaaatcag
      781 gtaagaataa aataccttct tatcgcttag caacctttga agttcgtcat tcaaggtcga
      841 aaaagttgtt aaaccgccac aaaatcctgt tgctagaata gcatatactt ccttagactc
      901 tacatgattg tagaatactc caattaaaaa acaacctaaa agattagcta tgagcgttcc
      961 gagtggcaat ttagaagctt gattatagcg agaaaagaaa taccgtacga gtgctccgaa
     1021 accacaagca attgcaagat agacgattac catttcttcc tccctagaaa ataagctaag
     1081 agtaggcctc caccgatgct caaaagcaaa tacaggacta aactaaaata acgccctgta
     1141 tcaagcagct tcacagtatc aagcattagg ctggaaaaag ttgttaaacc tccgcaaaaa
     1201 cccgtcccca gtgctaaaat caagccctta ctagtcccct tatagaccag atagcccttg
     1261 acaagaaaaa tcaagcagaa aattcccaga tagttgacaa agagagtgcc ccaaggaaag
     1321 tctggactgg ctggtaacca ggtggaaact agatatcgga caagtccgcc caacatagca
     1381 gctagaaaaa ttcctagcgg ataaaattgt tcttttttca tttgatattt tgatcctgat
     1441 aatcacgcga acgtttgagt atgtccgaaa aagtcgctac aatagtctcc tgatagcgct
     1501 tgtcctctac acgactccta accttttcaa aaataacttc ttctctctta gtatctaaaa
     1561 tcggtttacc tgaagctttc ttataagcaa ccaccccctc aactaaatgc attcgttctt
     1621 ctaacagttt gacaatttgg tcgtcgattt gatcgatttc ttgacgaata atatctaaat
     1681 ccatacagtc tcctccttta tttgaattat tgtatcaaaa agccatcaat taggctagta
     1741 aaatcccaac tctcgataag aaaaatgcac tgattgatta catcagcgca cttttatgtt
     1801 tatcctactt tagcagccaa ttcttctgcg aattgttcca agcgttcaat atcttcttcc
     1861 tcggcagaaa gatcaacttt aacacactct gaaccttttt ctgctcctgt tgacacaaag
     1921 acacggtcaa agtcatcaac agccttacag aattcatcgt agaaggtatc tcctgagccg
     1981 accactccgt agattttgcc attcaagttg agatctgcta ggtcttcgta gaagtccatc
     2041 atctcatctg gcaattcacc atcaccataa gtataggtcg caacgatagc gatgtctgct
     2101 tccaagaagt ctgaagcgtc aacagttgta cattcatcaa catcaacatc caagcccaag
     2161 tcacgtaatt tgtctgctac aatatctgca atttcttcgg tattaccggt catactggca
     2221 aatacaattt ttgctaatgc catatcgtcc tcctcaattt atctttttcc attatatcac
     2281 atctttttca ttttaaaaat aaaaattctt gtgctacaat gaaatgagaa agaattgagg
     2341 ttatttatgg agatttgcca acaaatttta gaaaaaatta aagaatacga caccattatc
     2401 attcatcgtc atatgaaacc agaccctgat gccttgggaa gtcaggtggg attgaaagcc
     2461 ttgctggaac atcatttccc agaaaaaacc atcaaagccg tcggttttga tgaaccaact
     2521 cttacttgga tggctgagat ggatcttgtt gaagatagag cctaccaagg cgcacttgtc
     2581 atcgtctgtg atacagctaa tactgctcgt atcgatgata agcgctatag tcaaggtgat
     2641 tttctcatta agattgacca ccatccaaat gatgatgtat acggtgacct gtcttgggtc
     2701 gatactagtt caagtagcgc tagcgagatg attaccctat ttgcccaaac aacccaacta
     2761 gccttggcag atcgcgatgc tgagttgctc tttgcaggaa ttgtcggtga tacaggtcgc
     2821 ttcctctacc cttctaccac tgcacggact cttcgcctgg ctgcttattt gagagaacat
     2881 aactttgact ttgcggctct cactcgcaaa atggacacta tgagctacaa aattgctaaa
     2941 ctgcaaggct acatctacga ccatctggaa gtggatgaaa atggtgctgc tcgcgttatc
     3001 ctgagtcaag aagtcttgaa acaatacaat gtaaccgatg ctgaaactgc agccattgtc
     3061 ggtgcacctg gacgcattga cagagtgagt ctctggggaa tttttgtcga acaggctgat
     3121 ggccactacc gagttcgctt acgcagtaaa atccatccta tcaatgaaat cgccaaggag
     3181 catgatggtg gaggccaccc tctagcaagt ggtgctaatt cctatagcct agaagaaaac
     3241 gaaatcatct accaaaagtt aaaaaacttg cttaaaaact gataaaatac ttgccaaact
     3301 tttcagaatc tgatagacta gtatggtaac aatctatggc tcgcaaagag accatggcag
     3361 aaaggaaata ttgcaaaatg aaaaaagata tccatccaga atatcgccca gttgtcttca
     3421 tggacacaac tactggttac caattcctta gcggttcaac aaaacgctct aacgaaacag
     3481 ttgagttcga aggcgaaact tacccattga tccgtgtgga aatttcatca gactcacacc
     3541 cattctacac tggacgtcaa aagttcactc aagcagatgg acgcgtggat cgtttcaaca
     3601 aaaaatacgg tctcaaataa tgataagaga acagtttcgg ctgttctttt ttgtttcttg
     3661 aaatcaactg ctgttttcat gttccagact catctgtagg ttcggtttcc atgctactag
     3721 gcagaaagga aatagctgtt tcaacacgtc cataatgagc tatactattg taacgaacca
     3781 cactttcatt gatggtccaa gtggaattca ttttcttaaa agcttctcgg actttttcca
     3841 aatctttgga ggcaatggcc tgctctaagg tttcaaaacg aggacttata ctcatctgct
     3901 ttcacaaagc attctagtcc atctccgatt aacgatggac tttatcacct ccttctccag
     3961 tccttgtatg acatcttgaa gttgattcat gatatcttcc aaagttcgaa aggctttatt
     4021 cttaaatcca cgtttacgaa tctctttcca cacttgttca atgggttcat ctctggtgtg
     4081 tatggaggaa taaaggtaaa atcaatatta gtcggaatat ttaaggtact tgatttatgc
     4141 catatagcat tgtccataac gagtaaaagg ataagcttgt gaaagctctt ctaaaaaggc
     4201 gttcatccac actccttttt agaagcctga aataaggcat caattgtaac aaattctcct
     4261 gcctctgcag ccttcaaatg gcgggcaaga aagactttct cttcctcaac tgtcatatat
     4321 gcatggttac gaccaccacg tgtttcttga aggagagagt cgagtccgaa ctcctcatat
     4381 ttttttacat ttcgccaaat cgttgtttga ttacagtcta aaagctctat aatctcttta
     4441 taagatttgc ccatcagacg aaatatagta gattgaaact agaatagtac acctctactt
     4501 ctaaaacatt gttagaaatc gatttgactg tcctga
//
LOCUS       DAVKJD010000103         4450 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKJD010000103 DAVKJD010000000
VERSION     DAVKJD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.103"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(56..1066)
                     /locus_tag="VW157_001856"
     CDS             complement(56..1066)
                     /locus_tag="VW157_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU9553109.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1215..2384
                     /locus_tag="VW157_001857"
     CDS             1215..2384
                     /locus_tag="VW157_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9553110.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2381..3151
                     /gene="recO"
                     /locus_tag="VW157_001858"
     CDS             2381..3151
                     /gene="recO"
                     /locus_tag="VW157_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9553111.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3148..4140
                     /gene="plsX"
                     /locus_tag="VW157_001859"
     CDS             3148..4140
                     /gene="plsX"
                     /locus_tag="VW157_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9553112.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4146..4379
                     /locus_tag="VW157_001860"
     CDS             4146..4379
                     /locus_tag="VW157_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9553113.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
BASE COUNT         1299 a          849 c         1139 g         1163 t
ORIGIN      
        1 aacatatata cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtattaga
       61 aaatgccgat aagggtctgc ataccaagag tggtgaggat gatggcaatc cagcagatgg
      121 ctccaagaag aatggatttt ccactggatt tgaccatagc gaccagattg gttttgagac
      181 cgatagcact catggccatg acaatgagga atttagagag ttgtttgaga ggggtaaaga
      241 aactactaga cacaccgaga gaggtcagaa gggtggttag gagcgatgca aggataaagt
      301 aaaggataaa aagtgggaag acttttttca gttgtaggct ttgcttgttt tcttgttggc
      361 gactttgcca gtaggataga aagagcgtga tagggataat ggccaaagta cgtgtgagtt
      421 taacaatggt tgcagactcg agggtattgc tttggtaaag actgtcccaa gcgctggcgg
      481 cagccgttac agaggaagtg tcgttgaccg cagtccctgc aaagagggcg aagccttcat
      541 tggatagatg aagccaggtg ccgagggttg gaaagatgag cgcagccaag acattgaaga
      601 aaaagataac ggaaatggct tgggctactt ccttttcctt agcatcaata acgggcgctg
      661 tcgctgcaat ggcagaaccc ccacagatag aagaacctac tccaaccaag gtagccagtt
      721 ttgtatccag tgcaaagaaa cgctggaaga ggtaggcaat aatcagagct attgagatag
      781 tggacaggat gacagggagt gaagattggc caactgcgaa gacctgcgag atattgagac
      841 caaaaccaag caagacaacg gcatattgga gcaacttctt ggaactaaag gtcaaaccag
      901 catccagttg tttatatgac gagagaaagg gatgtaggag catgcctatg aaaatggcaa
      961 aaacgggcgc gccaatcaca gggaagaatc ctcctaagta ccaagatacg atagaaatga
     1021 gaaggcaggc caagatacct gctccatttt ttgatagaaa tgacataaaa acctccaaat
     1081 aaaatctatt accattatag acctataaac aggaaaagta aaatagaaag tggaaagcta
     1141 ttctctaatg tatttttgcg gtcggggggc ttttgtagta taatagagat acgttttgaa
     1201 agtaggaggt atctatggac ttaactaagc gctttaataa acagttagat aaaattcaag
     1261 tttcgttgat tcgtcagttt gaccaggcta tttcggagat tcctggggtc ttgcgtttga
     1321 ccttggggga acctgatttt acaacgccag accatgtcaa ggaggcggcc aagcgagcga
     1381 ttgatcagaa ccaatcctac tatacaggga tgagtggtct gctgactcta cgtcaggcag
     1441 ccagtgactt tgttaaggaa aagtaccaac tggactatgc tcctgaaaat gaaatcttgg
     1501 ttacaattgg ggcgacagag gctttatctg cgactttgac ggctattttg gaagagggag
     1561 acaaggtact tttgccagct cctgcttatc caggctatga accgattgtt aacttagttg
     1621 gggcagaaat tgttgagatt gatacgactg aaaatggttt tgtcttgact cctgagatgt
     1681 tggagaaggc cattttggag cagggtgata agctcaaggc ggttattctc aactatccag
     1741 ccaatccgac aggaattacc tacagtcgag agcagttaga ggccttggca gctgttttac
     1801 gcaagtacga aatttttgtt gtctgtgatg aggtttactc agaattgacc tacacaggcg
     1861 aagcccatgt gtctctagga acgatgttga gagaccaggc tattattatc aatggtttgt
     1921 ctaaatcgca tgccatgaca ggttggcgtt tggggctgat tttcgctcct gcggccttca
     1981 cagcccagtt aatcaagagt caccaatact tggtcactgc cgcaaatacc atggcgcaac
     2041 atgctgcggt agaagccttg acggctggta aaaacgatgc ggagcccatg aagaaggaat
     2101 atatccaacg tcgggactat atcatcgaaa aaatgactgc tcttggtttt gagattatca
     2161 aaccagacgg tgccttctat atttttgcta aaattccagc gggctacaat caagactcct
     2221 ttgcttttct gaaggatttt gctcagaaga aggccgttgc ctttatccct ggtgcagcct
     2281 ttggacgtta cggggaaggc tacgtccgcc tatcttatgc agccagcatg gagactatca
     2341 aagaagccat gaaacgactt gaggagtaca tgagagaagc atgattcagt ctatcacgag
     2401 tcaaggcttg gtgctttaca atcgcaattt tcgtgaggat gacaagctcg tcaaaatttt
     2461 tacagagcag gttggcaaac gcatgttttt tgtcaaacac gctggtcagt ctaagctggc
     2521 gcctgttatt cagcccttgg tgctggcacg atttctcttg cgaatcaatg atgacggact
     2581 cagttacatc gaagactatc atgaggtcat gacttttccc aagattaata gtgacctctt
     2641 tgtcatggcc tatgcgacct atgtggcagc tcttgcagat gctagtttgc aggacaatca
     2701 gcaggatgct cccttgtttg cttttttgca aaagactttg gagttgatgg aagcaggctt
     2761 ggattatcag gttttgacca atatttttga aattcaaatt ttgactcgat ttggaatcag
     2821 cctcaatttt aatgagtgtg tcttctgcca tcgggttggt caggcttttg acttttcttt
     2881 caaatatgga gcctgcctct gtccagagca ttatcatgag gataagagac gttgtcatct
     2941 caatcccaat atcccctatc tgctcaatca atttcaagct attgattttg agactttgga
     3001 gaccatttcg ctcaagcctg gaatcaagca agagctacgc caatttatgg atcaattata
     3061 tgaagagtac gttgggattc acctaaaatc aaagaaattt attgattccc tagcagactg
     3121 gggacaatta ctaaaagagg aaaagaaatg aaaaaaatcg cagtagatgc catggggggc
     3181 gattacgcac ctcaggccat tgttgagggt gtcaatcaag ccctatctga cttttcagat
     3241 atcgaggttc aactttacgg agatgaagct aaaatcaagc aatatctgac agcgacagag
     3301 cgcgtcagca ttatccatac ggatgagaag attgattcgg atgatgaacc tacgagagct
     3361 attaggaata agaaaaatgc cagtatggta ttggcagcca aggctgtcaa agatggtgaa
     3421 gcagacgctg tcctttcggc tgggaataca ggtgccttgt tggcagcagg attcttcatc
     3481 gtgggtcgta tcaagaatat cgaccgtcct ggactcatgt ctaccttgcc taccgttgat
     3541 ggaaaaggtt ttgacatgct agaccttggt gccaatgcag aaaatacagc ccagcacctc
     3601 catcaatatg cggttctagg ttccttctat gctaaaaatg tccgtggcat tgcgcaacca
     3661 cgcgttggtt tgctcaacaa cggaacagag agtagcaagg gcgacccgct tcgtaaggaa
     3721 acttatgaat tactggcggc tgatgaaagt ttgaacttta tcggaaacgt ggaagcgcgt
     3781 gatttgatga atggcgttgc agatgttgtt gtggcagatg gtttcacggg aaacgctgtg
     3841 ctcaaatcca tcgaagggac agctatggga atcatgggct tgctcaagac agctattaca
     3901 ggtggtggtc ttcgagcgaa actaggtgcc ctccttctca aggacagcct cagtggtttg
     3961 aaaaaacagc tcaattattc agatgttggt ggagcggtct tgtttggtgt taaggcacct
     4021 gttgtcaaga ctcatggatc aagcgatgcc aaggctgttt atagtacgat tcgtcagatc
     4081 cgtaccatgc tagaaacaga cgtggttgcc cagactgcgc gtgaattttc aggagaataa
     4141 aagagatgac agaaaaagaa atttttgacc gtattgtgac cattatccaa gagcgacagg
     4201 gagaggactt tgtcgtgaca gaatccttga gtctgaaaga cgatttggat gcggattctg
     4261 ttgacttgat ggagtttatc ttgactctgg aagatgaatt tagtatcgaa atcagcgatg
     4321 aagaaattga ccaactccaa aacgtaggag atgtggttaa aatcattcaa ggaaaatagc
     4381 aatcggagtt ccaagtcaac ggaagtagat ggtttttaga aatgagaaat atcggacaag
     4441 ctggtaaaat
//
LOCUS       DAVKJD010000104         4422 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKJD010000104 DAVKJD010000000
VERSION     DAVKJD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.104"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..609)
                     /locus_tag="VW157_001861"
     CDS             complement(<1..609)
                     /locus_tag="VW157_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9553114.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNK"
     gene            complement(791..1753)
                     /gene="nrdF"
                     /locus_tag="VW157_001862"
     CDS             complement(791..1753)
                     /gene="nrdF"
                     /locus_tag="VW157_001862"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9553115.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(1941..4100)
                     /gene="nrdE"
                     /locus_tag="VW157_001863"
     CDS             complement(1941..4100)
                     /gene="nrdE"
                     /locus_tag="VW157_001863"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9553116.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKDLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(4181..4399)
                     /gene="nrdH"
                     /locus_tag="VW157_001864"
     CDS             complement(4181..4399)
                     /gene="nrdH"
                     /locus_tag="VW157_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9553117.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
BASE COUNT         1274 a          828 c          913 g         1407 t
ORIGIN      
        1 tttattagaa ttatttaaag cgatgcgttg agcctctccc tcttcctcgc taaaagtagc
       61 tagagctccg ttttgaatac cattacagct aacgaaagct ttagaaaatt ggagattaga
      121 gagattttgt agggtcaatg tgccaacaaa agcacctgta atatcgcgat aatttccacc
      181 tattaaaatc aaatctgtta attttcgttc gcttaaaatc agaaaaacag gtagactgtt
      241 ggttacgacg cggatattgt caataggcaa ctcacgcgca aaaaactcta atgttgttcc
      301 tggtccaatg aaaatagttt ctctttcttc tactagactg cctgcaaaat gggctatttc
      361 ttgtttttct gccgtttgga gggcttgttt ttcaatattt gatcgctcat tagtcaaaag
      421 ggagttggtt cgaagttttt cagctccacc atgcacacga atcagcaaat ctttatcagc
      481 taattcctgt aaatagcgcc ttgcagtcat atctgaaacg gctatttcgt ccataatctg
      541 tttaaccgtt atggttcctt tgctatttac tatctctaaa attttggtta atttttcttg
      601 tttgagcata ttatcacctc gtttcctact actatcttac cataaacaaa ctcatcattc
      661 aaatacaaaa acaacaaaat gaaacaaaaa caaaaatatc gaagtttgtt ttcaaaactt
      721 tcgatatttt tgttgggtta taactttgat gtttctagtt tactttttga tgattgagag
      781 tggtggagaa ttagtctaaa ccgtagttat agtcatcgtc ttgcatggct tcaacttcgc
      841 caagaaggta accatttccg acttgagaga agaagtcgtg gttggaagtt cctgttgaaa
      901 taccgttcat aacgattggg ttgacatctt cagctgaatc tgggaaaagt ggatcttgtc
      961 ccatgttcat gagagcttta ttggcattgt agcgaaggaa ggttttaacc tcttcagtcc
     1021 aaccaacacc gtcataaaga ctctctgtgt agccttcttc attttcataa agagtataga
     1081 gtaggtcgta catccattct ttgagttttt cttgctcttc ttcaggtaat tcattgaaac
     1141 caagttggaa tttgtaacca atgtaagttc cgtgaacaga ctcgtcacga ataatcaatt
     1201 taatgatttc tgcaacgttg gctagtttgt tgttaccgag atagtagagg ggagtgaaga
     1261 aaccagagta gaagaggaag gtttcgagga agacgctggc aactttcttt tcaagtgggc
     1321 tgccgtttag gtagatttcg ttgacaatct cagccttctt ttgtaggtaa ggattggtat
     1381 tggtccattc gaaaatttct tcaatctcag ccttagtatt caaggtagaa aagattgatg
     1441 agtaagattt agcgtggaca gattccataa attggatgtt attgaaaaca gcttcctcat
     1501 gtggtgtacg gatgtctgcg cgaagggctt gaaccccagt ttcagattgc atagtgtcaa
     1561 gaagggttaa accaccaaaa acttttccga ccaagtcttt ctctttgtta gatagctttc
     1621 tccagtcatc caagtcgttt gataagggaa tacgtgtatc gagccaaaat tgctccgtca
     1681 gtttttccca agttgatttg tcgatgacat cttcgatggc attccagtta atggctttgt
     1741 agtaagtttc catttaaaat ctctttctgt gtttagtatt gcgaactcac aattatttct
     1801 actttaccat aattctatag gagtatcgca caaaaagtcg gaagtccgac ttttaaaatg
     1861 ttacataaat tatgttatga catagtagat ttgattttat cagtgctgct tagggaaaaa
     1921 taatgtttct atgctggaaa ctaaatcaca cagctttcac attggttggc gccgacttct
     1981 ccaccgtcat ctgtaaaggt acggacgtag tagatagact tgattccctt gttaaaggca
     2041 tagttacgaa ggatggacaa atcacgtgtc gtttgtttat tttccctctt ccattcgtaa
     2101 aggtcttttg gaatgtcact gcgcatgaag agggtgagtg aaagtccttg atccacgtgt
     2161 tcagtcgcag cagcgtaaac atcgatgact ttacgcatat ccatatcgta ggcagaagtg
     2221 tagtaaggaa tggtttctgt agacaagcca gcagcagggt agtagatttt accaattttc
     2281 ttctcttggc gttcttcgat acgttgcgta atcgggtgga tagaagcaga aacgtcgttg
     2341 atatagctga tagaaccatt tggcgctaca gcaaggcgat tttggtggta aagaccatct
     2401 tcttgaacct tgtcgcgaag ttcagcccaa tcagcaacac cagggataaa aacatttttg
     2461 aagagttctt taacacggtc tgatgttgga acaaattcac cagttacata cttgtcaaag
     2521 taacttccgt tagcatagtc tgatttttca aagttgtgga aggtaatacc acgttcacgc
     2581 gcaatattgt ttgactctac caaggtccag tagttcataa gcataaagta gatgcttgta
     2641 aattcaacag actcaggtga accatattca atgagttgtt gggcaaggta gctgtgcagt
     2701 cccatggcac cgagaccaaa ggtgtgggct tggctatttc catggtcaat cgttggtaca
     2761 gctacgatat gtgaactatc tgtaacgaaa gtaagggcac gaaccatagc acggatagaa
     2821 cgaccaaaat caggtgaagt catcatgtta acgacgttag ttgaacccag gttacatgaa
     2881 acatctgttc ccatttgaag gaattcttga gcatcgttga tcaagcttgg ttcttgaact
     2941 tgaagaatct cagaacacaa gttactcatg ataatctttc catcaacagg atttgcacgg
     3001 ttagccgtat cgatgttgac tacataagga tagccagact cttgttgcaa tttagagatt
     3061 tcagtttcca aatcccgcgc cttgattttt gtcttgcgaa tatttggatt tgcgaccaat
     3121 tcatcgtatt tttcagtaat gtcgatgtaa ttgaatggca caccgtattc tttttctaca
     3181 gagtaagggc tgaagaggta catttcttca tttttacgag ccaattcgta gaatttatca
     3241 ggtactacaa caccaagtga tagagtcttg acacgtactt tttcatcagc gttttctttc
     3301 ttagttgaaa ggaaagcgat gatatctggg tgaaagacgt tgaggtagac aacaccagca
     3361 ccttgacgtt gccccaattg gttggagtaa gagaagctgt cttcaaaaag cttcataaca
     3421 ggaacgacac ctgaagcagc tccttcatag cctttgatag gtgcaccagc ttcacgaagg
     3481 ttgctgaggg taattcccac accaccacca atacgtgaaa gttgaagagc tgagttgata
     3541 gaacgtccga tagagttcat atcatccgtc acttggatta ggaaacaaga taccaactcc
     3601 ccacggcgag cacgtccagc attcaagaag gaaggagtag caggttggta gcgttggtgg
     3661 atgatttcat tggcaatatc gattgcaaca gcttcattcc catcagcgaa ataaagggcg
     3721 ttgaagaaga cacggtcttc catattttca agatagtatt caccgtcatt agtctttaag
     3781 gcatattgat tgtaaaattt ataagctgcc atgaatgact tgaattggaa gttttggtct
     3841 ttgataaatt gagccaattc ttccaagaac tctggacggt atttcttgat aaaggctgtt
     3901 tcgatgtagt tgtgttcaat gaggtaattg attttgtctt tgattgaatc aaaaaccata
     3961 gtgtttggaa ctacattttc tttaaagaaa gcatccaagg cttccttgtc tttatgaagc
     4021 atgatttgtc cattaacagg acggttaatt tcgttattaa gacggaagta agtcacgtct
     4081 tcaagatgtt ttaatcccat aaaatttccc ttatctaatt acaaaagaaa ggcttctaag
     4141 ttagccctaa aagcagtttc ttctggatga tgtactaaga ttatgctaat tgtttcagtt
     4201 ttcctggttg gaaacctgaa aagacttcag ttggtgtttg gataacagga gctgcgctaa
     4261 aaccgagctc tttaacttga tcgacgtact caggttgctc atcaagattg atttcacgat
     4321 aagagacatt attactgtcc aagaaacgct tggtcatttt acattggaca caattgtttt
     4381 tagaataaac ggttaccatt gtgtaactcc tcttcaaaat tt
//
LOCUS       DAVKJD010000105         4351 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKJD010000105 DAVKJD010000000
VERSION     DAVKJD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.105"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            319..2820
                     /locus_tag="VW157_001865"
     CDS             319..2820
                     /locus_tag="VW157_001865"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9553118.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWPESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVANYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVAEAAYTEDGLHVNSDFLDGLNKEEA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTRADGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKSVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFVQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKIMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIVVPNKLVNIVVK"
     gene            3014..3709
                     /locus_tag="VW157_001866"
     CDS             3014..3709
                     /locus_tag="VW157_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9553119.1"
                     /translation="MPVNEYGQMIGESMEAYTPGELPSFDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVANLEELVTLLNQMLARKDRFYYAIIDKATGKALG
                     TLSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFDELNYRRYEWKCDAL
                     NLPSRRAAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDKDWPKVKSRLEIWLRPENF
                     DKNGQQYKSLREF"
     gene            3721..4137
                     /locus_tag="VW157_001867"
     CDS             3721..4137
                     /locus_tag="VW157_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9553120.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPQMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPSYQRQGIGSSLMKQALEDFKEAYQVQ
                     LATEQTEKNVGFYRSMGFETLSTYDCTGMIWVNKEK"
BASE COUNT         1286 a          830 c         1005 g         1230 t
ORIGIN      
        1 agattcagtc tactatacgt tcttcataaa tcaaaaagcg acataggtag tcggctattt
       61 attgtgaata cattaattag cattccagtt ttatcttcgg tctaaaataa gtattttgtg
      121 ctatacgaga taagcttctt gacttactcc ttgatttact ccttgattta ctgcataaca
      181 atgggataaa aagtgggaga tagagcaatt catagtcatc aaaattaatg agatacagta
      241 tacagttttt cctttaaaca catttcaaat tccctcaaaa atggtataat agtaacatca
      301 caaaattgga gagagaccat gagtttttac aatcataaag aaattgagcc taagtggcag
      361 ggctactggg cagaacatca tacatttaag acaggaacag atgcatcaaa acctaagttt
      421 tatgcgcttg atatgttccc ttatccgtct ggagctggtc tgcacgtagg acacccagaa
      481 ggttatactg caaccgatat cctcagtcgt tacaaacgtg cgcaaggcta caatgtcctt
      541 cacccaatgg gttgggatgc ttttggtttg cctgcagagc aatacgctat ggatactggt
      601 aatgacccag cagaatttac agcggaaaac attgccaact tcaaacgtca gatcaatgcg
      661 cttggatttt cttatgactg ggatcgtgaa gtcaacacaa cagatccaaa ctactacaag
      721 tggactcaat ggattttcac caagctttac gaaaaaggct tggcctatga agctgaagtg
      781 ccagtaaact gggttgagga attgggaact gccattgcca atgaagaagt gcttcctgac
      841 ggaacttctg agcgtggagg ctatccagtt gtccgcaaac caatgcgcca atggatgctc
      901 aaaatcacgg cttacgcaga gcgcttactc aatgacttag atgaacttga ttggccagag
      961 tctatcaagg acatgcaacg caactggatt ggtaaatcaa ctggtgctaa tgtaaccttt
     1021 aaagttaagg gaacggacaa ggaattcaca gtctttacca ctcgtcctga tactcttttc
     1081 ggtgcgactt tcactgtctt ggctcctgaa catgaattag tagacgctat cacaagttca
     1141 gagcaagcag aggctgtagc aaactataaa catcaagcta gcctcaaatc tgacttggct
     1201 cgtacagacc ttgccaaaga aaaaactggt gtttggactg gtgcttatgc catcaaccct
     1261 gtcaatggta aggaaattcc aatctggatt gccgactatg ttcttgctag ttacggaaca
     1321 ggtgccgtta tggccgttcc agctcacgac caacgtgact gggaatttgc caaacaattt
     1381 gaccttccaa tcgtcgaagt gcttgaaggt ggaaatgtag cagaagctgc ctatacagag
     1441 gatggtcttc atgtcaattc agacttccta gatggactca acaaagaaga agctattgcc
     1501 aagattgtgg cttggttgga agaaaaaggc tgtggacaag agaaggttac ctaccgtctc
     1561 cgcgactggc tctttagccg tcaacgttac tggggtgaac caattcctat cattcattgg
     1621 gaagatggga cttcaacagc tgttcctgaa agtgaattgc cacttgtctt gcctgtaacc
     1681 aaggatatcc gcccttcagg tacaggggaa agtccattgg ctaacttgac agattggctt
     1741 gaagtgactc gtgcagatgg tgtcaaaggt cgtcgtgaaa ccaacactat gccacaatgg
     1801 gcaggttcaa gctggtacta cctccgctat attgacccac acaatactga gaaattggct
     1861 gatgaggatc ttctcaaaca atggttgcca gtagatatct acgtgggtgg tgcagagcat
     1921 gctgttcttc acttgctcta cgctcgtttc tggcataaat tcctctatga cctcggtgtt
     1981 gttccaacta aggaaccatt ccaaaaactc tttaaccaag gaatgatttt gggaacaagc
     2041 taccgtgacc accgtggggc tcttgtggca accgataagg ttgaaaaacg tgacggttct
     2101 ttcttccatg tggaaacagg agaggagttg gagcaagcgc cagccaagat gtctaaatct
     2161 cttaagaatg ttgtcaaccc agacgatgtg gtggagcaat acggtgccga tacccttcgt
     2221 gtctatgaaa tgtttatggg accacttgat gcttcgattg cttggtcaga agaaggtctg
     2281 gaaggaagcc gtaaattcct tgaccgtgtt taccgtttga ttacaagtaa agaaatcctt
     2341 gcggaaaaca atggcgctct tgataaggtt tacaatgaaa cagtcaaatc tgtcacagag
     2401 caaattgagt ccctcaaatt caacaccgcc attgcccaac ttatggtctt tgtcaatgcg
     2461 gctaacaagg aagacaagct ctatgttgac tacgccaaag gcttcgttca gttgattgca
     2521 ccatttgcac ctcacttggc agaagagctc tggcaaacag ttgcggcaac aggtgagtca
     2581 atctcttacg tgacttggcc aacatgggat gaaagcaaat tggttgaaga cgaaatcgaa
     2641 atcgtcgtcc aaatcaaagg taaagttcgt gccaaaatca tggttgctaa agacctatca
     2701 cgcgaagaat tgcaagaaat tgctctagct gatgaaaaag tcaaagcaga aattgatggt
     2761 aaggaaatcg tgaaagtgat tgtggtaccg aataaattgg ttaatattgt tgtgaaataa
     2821 ccaatttatt tgctggtttc aaatctcgga cagtttccta aactgtttga gccagttaaa
     2881 ctcgttaata ttgttttgaa ataagatagg agtccttcag agtagaatct gggggatttt
     2941 ttgaatcttc ttatgaaagt atgatatact atgggcaact ataaagtttg aaaagtgaaa
     3001 taaggagaat aagatgccag taaatgaata tggtcaaatg attggggagt caatggaagc
     3061 ttatactcca ggtgaattgc cttcttttga tttcttagaa gggcgttatg ctcgaataga
     3121 agctctttca gtggaaaagc acgcggagga tttattagct gtttatggcc ctgatactcc
     3181 tcgggagatg tggacctacc tctttcagga gccagtggca aatctggagg agctggttac
     3241 tcttttaaat cagatgttgg ctcgtaagga ccgtttttac tatgcaatta tagacaaggc
     3301 aactggtaag gctttgggaa ctttgtctct catgcgtatt gaccagaata accgagtaat
     3361 agaagtggga gctgtcactt tttctccaga gctcaggggg acacggatag gaacagaagc
     3421 tcagtatctt ttggctcgct atgtctttga tgagcttaac tatcgtcgct atgagtggaa
     3481 atgcgatgct cttaacctgc catccagacg agctgcggag cgtttgggat ttgtctacga
     3541 aggaaccttt cgtcaggcag tggtttataa ggggcgtacg agggatacgg attggttgtc
     3601 tatgattgat aaggactggc ctaaagtcaa atctcgtttg gaaatatggt tgcgtcctga
     3661 aaactttgat aaaaatggac agcagtacaa gagcttgaga gaattctaag aggtgttgag
     3721 atgattacta ttaaaaagca agaaattgtc aagctagagg atgttttgca tctctatcag
     3781 gctgtcggtt ggacaaatta tacccatcaa cctcagatgc tggagcaggc cttgtctcat
     3841 tcattagtaa tttatctggc gcttgatggc gatgctgtgg tgggcttgat tcgtttggtt
     3901 ggagatggtt tttcatcagt ttttgtacag gatttgattg ttttacctag ctatcagcgc
     3961 caagggattg gtagctcctt gatgaaacag gctttagagg attttaaaga ggcttatcaa
     4021 gtccagctgg cgacagaaca gacagaaaaa aatgtaggat tttatcgttc tatgggtttt
     4081 gaaactttat ccacctatga ttgtacagga atgatttggg taaacaaaga aaaataacaa
     4141 aacttgtttt ttcttaagca aagtttaagg atggtctagt attatatagt cattaaataa
     4201 agacctccta actttattta ataaaatcct aaactttttt catcacaatc tcctaatgaa
     4261 gccacccaat caggtggctt ttttgcgtgg tgaggtgctg gtgatagaaa ttttttgcaa
     4321 aatagtaaaa ttttagaaaa gttaagctag t
//
LOCUS       DAVKJD010000106         4226 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKJD010000106 DAVKJD010000000
VERSION     DAVKJD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.106"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            635..862
                     /locus_tag="VW157_001868"
     CDS             635..862
                     /locus_tag="VW157_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553121.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            1150..4047
                     /gene="nanA"
                     /locus_tag="VW157_001869"
     CDS             1150..4047
                     /gene="nanA"
                     /locus_tag="VW157_001869"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEU9553122.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRIIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1421 a          706 c          920 g         1179 t
ORIGIN      
        1 atgtgtacta ttctagtttc aatctattat atttatagaa ttttttgttg ctagatttgt
       61 caaattgctt aaaataattt ttttcagaaa gcaaaagccg atacctatcg agtagggtag
      121 ttcttgctat cgtcaggctt gtctgtaggt gttaatactt ttcaaaaatc tctttaaacc
      181 acgtcagctt cgccttgccg tatatatgtt actgacttgg tcagttctat ctgccacctc
      241 aaaacggtgt tttgagctga ctttgtcagt cttatctaca acctcaaaac agtgttttga
      301 gcatcatgcg gctagcttct tagtttgctc tttgattttc attgagtata aaaacagatg
      361 agtttctgtt ttctttttat ggactataaa tgttcagctg aaactacttt caaggacatt
      421 attatataaa agaatttttt gaaactaaaa tctactatat tacaccatat tgaaagcgtt
      481 ttaaaaatga ggtataataa atttactaac acttataaaa agtgatagaa tctatcttta
      541 tgtatattta aagatagatt gctgtaaaaa tagtagtagc tatgcgaaat aacagataga
      601 gagaagggat tgaagcttag aaaaggggaa taatatgata tttaaggcat tcaagacaaa
      661 aaagcagaga aaaagacaag ttgaactact tttgacagtt tttttcgaca gttttctgat
      721 tgatttattt cttcacttat ttgggattgt cccctttaag ctggataaga ttctgattgt
      781 gagcttgatt atatttccca ttatttctac aagtatttat gcttatgaaa agctatttga
      841 aaaagtgttc gataaggatt gagcaggaag tatggtgtaa atagcataag ctgatgtcca
      901 tcatttgctt ataaagagat attttagttt aattgcagtg gtgtcctggt agataaacta
      961 gattggcagg agtctgattg gagaaaggag aggggaaaat tggcaccaat ttgagatagt
     1021 ttgtttagtt catttttgtc atttaaatga actgtagtaa aagaaagtta ataaaagaca
     1081 aactaagtgc attttctgga gtaaatgtct tatttcagaa atcgggatat agatatagag
     1141 aggatcagta tgaatcggag tgttcaagaa cgtaagtgtc gttatagcat taggaaacta
     1201 tcggtaggag cggtttctat gattgtagga gcagtggtat ttggaacgtc tcctgtttta
     1261 gctcaagaag gggcaagtga gcaacctctg gcaaatgaaa ctcaactttc gggggagagc
     1321 tcaaccctaa ctgatacaga aaagagccag ccttcttcag agactgaact ttctggcaat
     1381 aagcaagaac aagaaaggaa agataagcaa gaagaaaaaa ttccaagaga ttactatgca
     1441 cgagatttgg aaaatgtcga aacagtgata gaaaaagaag atgttgaaac caatgcttca
     1501 aatggtcaga gagttgattt atcaagtgaa ctagataaac taaagaaact tgaaaacgca
     1561 acagttcaca tggagtttaa gccagatgcc aaggccccag cattctataa tctcttttct
     1621 gtgtcaagtg ctactaaaaa agatgagtac ttcactatgg cagtttacaa taatactgct
     1681 actctagagg ggcgtggttc ggatgggaaa cagttttaca ataattacaa cgatgcaccc
     1741 ttaaaagtta aaccaggtca gtggaattct gtgactttca cagttgaaaa accgacagca
     1801 gaactaccta aaggccgagt gcgcctctac gtaaacgggg tattatctcg aacaagtctg
     1861 aaatctggca atttcattaa agatatgcca gatgtaacgc atgtacaaat cggagcaacc
     1921 aagcgtgcca acaatacggt ttgggggtca aatctacaga ttcggaatct cactgtgtat
     1981 aatcgtgctt taacaccaga agaggtacaa aaacgtagtc aactttttaa acgctcagat
     2041 ttagaaaaaa aactacctga aggagcggct ttaacagaga aaacggacat attcgaaagc
     2101 gggcgtaacg gtaaaccaaa taaagatgga atcaagagtt atcgtattcc agcacttctc
     2161 aagacagata aaggaacttt gatcgcaggt gcagatgaac gccgtctcca ttcgagtgac
     2221 tggggtgata tcggtatggt catcagacgt agtgaagata atggtaaaac ttggggtgac
     2281 cgagtaacca ttaccaactt acgtgacaat ccaaaagctt ctgacccatc gatcggttca
     2341 ccagtgaata tcgatatggt gttggttcaa gatcctgaaa ccaaacgaat cttttctatc
     2401 tatgacatgt tcccagaagg gaagggaatc tttggaatgt cttcacaaaa agaagaagcc
     2461 tacaaaaaaa tcgatggaaa aacctatcaa atcctctatc gtgaaggaga aaagggagct
     2521 tataccattc gagaaaatgg tactgtctat acaccagatg gtaaggcgac agactatcgc
     2581 gttgttgtag atcctgttaa accagcctat agcgacaagg gtgatctata caagggtaac
     2641 caattactag gcaatatcta cttcacaaca aacaaaactt ctccatttag aattgccaag
     2701 gatagctatc tatggatgtc ctacagtgat gacgacggga agacatggtc agcgcctcaa
     2761 gatattactc cgatggtcaa agccgattgg atgaaattct tgggtgtagg tcctggaaca
     2821 ggaattgtac ttcggaatgg gcctcacaag ggacggattt tgataccggt ttatacgact
     2881 aataatgtat ctcacttaaa tggctcgcaa tcttctcgta tcatctattc agatgatcat
     2941 ggaaaaactt ggcatgctgg agaagcggtc aacgataacc gtcaggtaga cggtcaaaag
     3001 atccactctt ctacgatgaa caatagacgt gcgcaaaata cagaatcaac ggtggtacaa
     3061 ctaaacaatg gagatgttaa actctttatg cgtggtttga ctggagatct tcaggttgct
     3121 acaagtaaag acggaggagt gacttgggag aaggatatca aacgttatcc acaggttaaa
     3181 gatgtctatg ttcaaatgtc tgctatccat acgatgcata atggaaaaga atacattatc
     3241 ctcagtaatg caggtggacc gaatcgtgaa aatgggatgg tccacttggc acgtgtcgaa
     3301 gaaaatggtg agttgacttg gctcaaacac aatccaattc aaaaaggaga gtttgcctat
     3361 aattcgctcc aagaattagg aaatggggag tatggcatct tgtatgaaca tactgaaaaa
     3421 ggacaaaatg cctataccct atcatttaga aaatttaatt gggacttttt gagcaaagat
     3481 ctgatttctc ctaccgaagc gaaagtgaag cgaactagag agatgggcaa aggagagatg
     3541 ggcaaaggag ttattggctt ggagttcgac tcagaagtat tggtcaacaa ggctccaacc
     3601 cttcaattgg caaatggtaa aacagcgact ttcctaactc agtatgatag caagaccttg
     3661 ttgtttgcag tagataagga agatatcgga caggaaatta ttggtatagc taaaggaagc
     3721 atcgaaagta tgcataatct tcctgtaaat ctagcaggtg ccagagttcc tggcggagta
     3781 aatggtagca aagcagcggt gcatgaagtt ccagaattta cagggggagt taatggtaca
     3841 gagccagctg ttcatgaaat cgcagagtat aagggatctg attcgcttgt aactcttact
     3901 acaaaagaag attatactta caaagctcct cttgctcagc aggcacttcc tgaaacaggg
     3961 aacaaggaga gtgacctcct agcttcacta ggactaacag ctttcttcct tggtctgttt
     4021 acgctaggga aaaagagaga acaataagag aagaattcta aacatttgat tttgtaaaaa
     4081 tagaaggaga tagcaggttt tcaagcctgc tatctttttt tgatgacatt caggctgata
     4141 cgaaatcata agaggtctga aactactttc agagtagtct gttctataaa atatagtaga
     4201 ttgaaataag atgtgaacaa ctctat
//
LOCUS       DAVKJD010000107         4098 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKJD010000107 DAVKJD010000000
VERSION     DAVKJD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.107"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            45..854
                     /locus_tag="VW157_001870"
     CDS             45..854
                     /locus_tag="VW157_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9553123.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            859..1560
                     /locus_tag="VW157_001871"
     CDS             859..1560
                     /locus_tag="VW157_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9553124.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1585..2502
                     /locus_tag="VW157_001872"
     CDS             1585..2502
                     /locus_tag="VW157_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEU9553125.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2530..4071
                     /locus_tag="VW157_001873"
     CDS             2530..4071
                     /locus_tag="VW157_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU9553126.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
BASE COUNT         1335 a          628 c          796 g         1339 t
ORIGIN      
        1 ttaaaataaa gaatgtaaac gtttacttca actaaggagc tcatatgtta ctgcaaaaag
       61 aactaattcc aatgatagaa gctaacttac caaatatggc atatgctgaa aaagacattg
      121 ctaaattctt cttaaaacag caacctctga atgattattc atctaaagca ttgtgcgaat
      181 accttaatgt atccaaagca acattgactc gatttgcgaa aaaatgtggt tttaaaggtt
      241 ttagacaatt cattttcaaa taccaagaga tgattcatga gaaagaaaag ttggcattat
      301 atacagaggc aacagaaaaa gttttatccg actatgagga aatgttgaga aaaacttaca
      361 cggttcttga tgaagttcaa cttgagcgta ttgctgagat gatagaaact gctgagcgtg
      421 tatatctcta cggtaaagga agttctgttc ttgctttaca agaaatgaag atgagattta
      481 tgcgtctcgg agtgattggt gaagtattat cagacgagga tatgattttg tggagtagct
      541 tactacttaa tgaaaattgc cttgtcattg gagcatccat ttcaggtcaa actgatattg
      601 tactagaagg tctacaaaaa gctgcagata aaggcgctaa aacagtttta atgactacaa
      661 gaaaatttga tgaagaagat tgtttctttg atgaactatt gttattagct tcgaccgatc
      721 atctctcgta tggcaatcgc atatcacctc agtttccaat acttttaatt acagactgct
      781 tattctctaa ttatctggaa agtccagaga gacaatatta ttacaatcaa actattatcc
      841 ataaggagga ataataaaat gagccaaatt tggactaaag aaaaatttat aagccaagtt
      901 caaggtggag tcattgtttc ttgtcaagct ttacctggtg aagcccttta taatgaagaa
      961 tttagcttga tgccttttat ggctaaagca gctttagagg caggagcagt gggcattcgc
     1021 gcaaattctg tgcgtgatat taaagcaatt cagaaagtag tagatttacc aataattgga
     1081 attatcaaaa gggattatcc acctcaagaa ccatatatta ctgctacgat gaaagaagta
     1141 gatgaacttg tagaatgcgg aacaacagtc attgcatttg atgcaacttt aagaccaaga
     1201 tatgatggct tagttgtcag tgaatttatc aaaaaaataa aagaaaaata tccgaatcaa
     1261 ttgctgatgg cggatgtaag taatttagat gaaggtctct atgcatttaa atcaggcgtt
     1321 gattttgttg gtacaacatt atcaggttac acaagtacaa gtgtacaatc agatgagcct
     1381 gattttgaac taatgaaaaa attggctgat tttaatattc cggtaattgc cgaaggaaaa
     1441 attcattatc cagaacaatt aaaaaaagct tatagtttag gtgttaccag tgtagtcatt
     1501 ggtggagcga ttacacgtcc aaaagaaatt gctcagcgat ttattaatgt catcaaataa
     1561 aaaacaatat aaaggagaaa tctgatgaaa gatttaacta aatacaaagg cgttatccct
     1621 gcattttatg cttgctatga tgaaaatggt gaaattagcc aagatcgtgt aaaatctctg
     1681 gtacaatatt tcattgacaa aggtgtaaaa ggtatctatg taaatggttc ttcaggtgaa
     1741 tgtatttacc aaagtgtaga agatcgtaaa caaattattg aagctgttat ggaagttgct
     1801 aaaggtaaat taacagttat caaccatatt gcatgtaata acacgaaaga tagtatcgaa
     1861 ttggcaaaac attcagaaag tgttggagtc gatgctattg cagctatccc acctatttat
     1921 ttcaaattgc cagagtattc aatcgcagca tattggaatg caatgagtga agctgcgtca
     1981 aatacagatt ttattatcta taatattcca caattggcag gggttgcgtt gactggtagt
     2041 ttgtatgcaa caatgcgtca aaatcctcgt gtgattggag ttaaaaattc ttctatgcct
     2101 gtacaagata ttcaaatgtt tgtagctgca ggtggagaag attacattgt attcaatggt
     2161 ccagatgaac aatatcttgg tggtcgcttg atgggagcag aagctggtat tggtggtact
     2221 tatggcgtta tgccagattt gttcttgaaa ttggaaagtt tgattcaaga acgagattta
     2281 gatacagcta aaaaacttca atatgctatc aatgaagtta tctataagat gatatcaggt
     2341 aaggcaaata tgtatgctgt agcaaaagaa gttttgcgtc taaatgaaaa acttgattta
     2401 ggttctgttc gtcaaccttt agaagcattg gcagaaggtg acttggaagt tgcaaaacaa
     2461 gcagcagaac ttattcaaca agcacgaaaa gaatttttat aataaataaa atcaatatgg
     2521 agatatgtta tgggcacaac aggatttaca ataattgact taattatctt gattgtttat
     2581 ttacttgcgg tgttggttgc aggtatctat ttctctaaaa aagagatgaa aggaaaagag
     2641 ttctttaaag gagatggttc ggttccttgg tatgttactt cggtatccat ttttgccaca
     2701 atgctcagtc cgatttcctt cttgggactc gctggtagct cttatgcagg tagctggatt
     2761 ttatggtttg ctcaattagg gatggtagta gctattccac tgacaattcg ttttatctta
     2821 cctatctttg cacggataga catcgatacg gcatatgatt acttggataa acgttttaat
     2881 tctaaagcac ttcgtattat ttcagcactc ttgtttatta tttatcaatt gggacgtatg
     2941 tctatcatta tgtacctccc atcagctggt ttatcagtat tgacaggaat tgacatcaat
     3001 attttgatta ttttgatggg tgtagttgca attgtttatt cttatactgg tggtctaaaa
     3061 tccgtattat ggacagactt tattcaaggt gtgattctga ttagtggtgt cgttttagct
     3121 ttatttgtac tgattgctaa tattaaaggt ggctttggtg cagtagcaga aacattagca
     3181 aacgggaaat tccttgctgc aaatgaaaaa cttttcgatc ctaacttgct ttcaaactcc
     3241 atctttttaa ttgtgatggg ttcaggcttt acaatcttgt cttcctatgc ttcatctcaa
     3301 gatttggttc aacgttttac tacaacacaa aatattaaga aacttaataa gatgttgttc
     3361 acaaacggtg ttttgtcact tgcaactgca acagtctttt acttgattgg tacaggcttg
     3421 tacgtattct atcaagtaca aaatgcagat agtgcagcta gcaatatccc tcaagaccaa
     3481 atctttatgt actttattgc ataccagtta ccagtaggta tcacaggttt gatcttggca
     3541 gcgatttatg cagcatctca atcaactatt tcaacaggtt tgaactctgt tgcaacttca
     3601 tggacattgg atattcaaga tgtcatttct aaaaatatgt cagacaatcg tcgtacgaaa
     3661 attgcacaat tcgtatctct agcagtaggt ttattctcaa ttggtgtttc cattgtcatg
     3721 gctcactcag atattaaatc tgcatacgaa tggttcaata gtttcatggg acttgtactt
     3781 ggtctacttg gtggtgtatt tattcttgga tttgtttcta aaaaagcaaa taaacaaggt
     3841 gcttatgcag cgctgattgt atcaaccatc gtcatggtat ttattaaata cttccttcct
     3901 ccaacagctg ttagctactg ggcatattca ttgatttcaa tctctgtatc agtagtttca
     3961 ggttatattg tatctgttct tactggaaat aaagtatctg cacctaaata tacaacgatt
     4021 catgatatta cagaaattaa agcggattca agttgggaag ttcgtcacta atagaaaaga
     4081 gaaaataata tgattatt
//
LOCUS       DAVKJD010000108         4094 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKJD010000108 DAVKJD010000000
VERSION     DAVKJD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.108"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(369..566)
                     /locus_tag="VW157_001874"
     CDS             complement(369..566)
                     /locus_tag="VW157_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9553127.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEASNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNPQNPCQILIKKLE"
     gene            complement(551..769)
                     /locus_tag="VW157_001875"
     CDS             complement(551..769)
                     /locus_tag="VW157_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9553128.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            complement(867..1652)
                     /locus_tag="VW157_001876"
     CDS             complement(867..1652)
                     /locus_tag="VW157_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9553129.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            complement(1654..2472)
                     /locus_tag="VW157_001877"
     CDS             complement(1654..2472)
                     /locus_tag="VW157_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9553130.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            complement(2474..3466)
                     /locus_tag="VW157_001878"
     CDS             complement(2474..3466)
                     /locus_tag="VW157_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9553131.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQNNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
BASE COUNT         1366 a          898 c          707 g         1123 t
ORIGIN      
        1 aaccatacgc acggcaaggc gacgttgacg tggtttgaag agattttcga agagtataag
       61 tccaaaactt tcgttaaccg ggatacatta taagcacatt cataccatca aaaatagtag
      121 caataaactt gaacaactac cagaaacacg aaaacaggca taattgatga aatatcagcg
      181 ctattaaaca atataaataa ccgctaagaa taaggcttaa cggttatctt ctacctatct
      241 attttttcaa ctcctcagtc gtttatctac ctataaccta ggctttacga gtgccttgct
      301 ctaccaactg agctaaatcg gcgattacac ttacagtatg gcactctgag aatagatgtc
      361 aagtaatatt attcaagctt tttaatcaaa atctgacaag gattttgcgg attccatagc
      421 tgagaaaaaa tctctaactt tttaaagcca agacctcgat aaaagtcatt tgttcgatca
      481 taatctttat tagaagcttc tgccactgtt ttgacctgca gataaccaac ttttttacga
      541 gcttcactct ctaaagtagc aagcaattgg ctcccaattt ttctaccttg ataagctttt
      601 ttacgccgag acaatcaatc tctgcacaat cttcactcga ataggataac cttacaaatc
      661 cagtcaaatc actctcctga taggcggtcc aaacttgcag tgtcgtggtt ccttctatat
      721 aggcttgtgt gctttctggg attccaaacc attctggcaa atccttcaaa acctcagcga
      781 caactgcctt ttttgatttt catccttgac ttcttcaatt acaaacatca taagttcctt
      841 tcttgatttt agtcttactt tagcttttac gaacccgcac tttcgtagga atctaagccc
      901 ttatcccaaa gtttaaggga aataacaaag aaaaccagag aaatcaacat caaacctcct
      961 acgttaaaga acacatcctt ttcctgtaag aaatagctag ctggatagta ggctgtaaag
     1021 gcgaaaggca cgataaagct aatcaaccaa cgaagaagag aattgtaaat agaaatcgga
     1081 tacttagcaa agtcattgaa catatagaag atgtaaatca tggcgcctga ctgcttagtc
     1141 caaaaggcga tactggctgt tgcgatttta agagaagtat aaatcaaggt cgcaaaagga
     1201 atacaaacta ggaaaagcag gaattttgga agagtccaaa caatgctggt cactgttgtt
     1261 cccaataaaa taccaccgac taagagttca cccaaggcat caatctgaaa ggtttcaact
     1321 aggatgtgaa agagaggatt gatgggacga gtcaaatact tgtcaaactc cccttttcgg
     1381 actaggcgtt gccctagtgc ccagagattg tcaaaaaaga gatggtccat tcccttggga
     1441 atcaaggaaa atccataaat gaaagctatc tcttgaaagg tccagccttc taggaatgga
     1501 atatgttgaa agatgacatt gagaaacaag agattcaagc cttgagccag aaagactccc
     1561 aagacaccaa ccacaaaatc caccttatat tccatgattt gtttgatgta ttgtctgata
     1621 aaaatcagat gcattcgttg atattttttc atactaacct ccttgaatgg tgataaagga
     1681 ctggacccgt ttccaaatca actgagacaa tcccaccatc actaagagcc agaagaactg
     1741 caaaaggagt gcctgaagaa tctgactggc atcgtatttt ccaacaatga tcataactgg
     1801 agtataaatc aaggatgaaa aaggcaaaaa ggaaagaata tctgaaacaa cctttggaaa
     1861 gaatgccaag ggaatcaaac tccccgacat aaaagccact atggaagtct taagtaggtt
     1921 ggaaccccaa agatttttaa acacaaaggc tgaaaatcca aagcaaatat taaagaaaaa
     1981 gttaatcaga taggcgagcg ttaagctaaa aagataaagg acagttaatc ctagcacctc
     2041 tacaatacct tgacccgata tgattttcat caagacaatg acacttaaaa atggaaggcc
     2101 aacgctgata aaaatcaacc acttggaacc aagctcggtg aaaagatagg aggccgcaaa
     2161 atgcactggt cgcaacaaac gcatgataat ggagccatcc ttgacctcct ccccaatcat
     2221 aaaggacgaa tcggatctag tcagaagatt ggtcacaaaa ctcatgatga tgtagagggt
     2281 gatatccgcc atactgaagc cctgaatcaa agactcttgc gaagaatcaa agacagcctt
     2341 ccagagataa aaggccacaa aagcccccat gacatcgcca atccgataga gaataaagtt
     2401 gactcggtaa gtaatcaact cctgaacccc tgcattgata aagggtttat aacgtctcca
     2461 caatttgatc atcctagagc tcctttcggt agaagcgacg gataatatcc tcaatatccg
     2521 tatccaccat cttcaaatcg cggatttcaa aatcagacag ggtttgcttg ataatgtcag
     2581 ctgactggta gcgagaacta tcaaattcaa tgttgaggct gtttccttgt ctatcaatgg
     2641 tcatatcaga cagaccttca tagtgagaga cgagatgact ttgacctggt agcagttcaa
     2701 aagagagagt cttcatctta ccaaaggtct ccttgagttg gctcaccgtt ccatcaaaaa
     2761 tctcttgccc cttgtcaatc atgaaaatcc gatcacaaag ttgctcaata tcactcaaat
     2821 cgtgagtggt caaaagaatg gtagtttctt cctcttgatt gatctgagta attgcccgac
     2881 gaatattatc cttaaccgaa acgtccaaac caatggtcgg ctcatctaaa aaaagaacct
     2941 tgggattgtg gagcaaggag gccgcaatat ccgcccgcat ccgttgtccc agtgaaagag
     3001 tccgcacggg atccttgata aagtccttca aatccaagac ttcattcaaa aagtccatac
     3061 gcttatgaaa gagcgagtct ggcacatcat aaatctcttt taagacagtg taggtctctt
     3121 gcagagccaa atcccaccat agctgggtgc gttgtccaaa gactacgcca atatctttga
     3181 cataatcttg ccgattgttc tggggaatct tgccgttaat ccgacaaaaa ccagatgttg
     3241 gtttcaaaat tcctgtcagc attttaatgg ttgtcgactt cccagcacca tttgccccga
     3301 taaatcctaa aatctgccct tttggaactt caaaggtcaa atccttgacc gcttcaaagg
     3361 tctgcttttc aggatgaata aaggagcgca aagccccctt caagcccggt tccttaacag
     3421 tcttcacaaa atttttctga agatgttcca cttctatcat tgccatatct atctccctat
     3481 cataaggaat agcaacattg tgtttttaac tacaaatatt ctaaatcctt actttctaca
     3541 ccttctacat tttattacta cagaagactt tataccaacc tattatagtc caataaaaaa
     3601 cagaatgcaa gcgtttgcct atggattgtg aaattaggat ttctctcatg aaataatact
     3661 tttaatcaaa aattctgttt aatagtttaa attaccaacc tactcatctt ctcaccagag
     3721 ggcggtatta actttgatca taaaatcgga tactccctct atccagaaaa tatgctcaga
     3781 atgattgtct ctaaacaata cctgctcttt tacctcattc atgaaaaaga agtccatacc
     3841 ctgagaatta tcaactcacg caccgactac ctaaaccaac tcgaccacct ctttcgtaca
     3901 tgtaggaaac tattttcctc acaaataatc tcattataaa atccaataaa ttttttagct
     3961 cacttgaata ttttctttgt atcgttttat cagcttcaat ctgatctaga cttgttacct
     4021 tggaacgact tgaaataacc tttataaaat aacacttaag gctttcatca tctcgtataa
     4081 atttttcaat agtg
//
LOCUS       DAVKJD010000109         4061 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKJD010000109 DAVKJD010000000
VERSION     DAVKJD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.109"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            43..1944
                     /locus_tag="VW157_001879"
     CDS             43..1944
                     /locus_tag="VW157_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9553132.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLGTNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
     gene            1947..2810
                     /locus_tag="VW157_001880"
     CDS             1947..2810
                     /locus_tag="VW157_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9553133.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKILVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            2890..>4061
                     /locus_tag="VW157_001881"
     CDS             2890..>4061
                     /locus_tag="VW157_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9553134.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYWSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEK"
BASE COUNT         1182 a          705 c          890 g         1284 t
ORIGIN      
        1 gattatcttg aatgccttag ggaaacatgc tatactactc ttatgattat tttacaagct
       61 aataaaattg aacgttcttt tgcaggagag gttcttttcg ataatatcaa cctgcaggtt
      121 gatgaacgag atcggattgc ccttgttggg aaaaatggtg caggtaagtc tactcttttg
      181 aagattttag ttggagaaga ggagccaact agcggagaaa tcaataagaa aaaagatatt
      241 tctctgtctt acctagccca agatagccgt tttgagtctg aaaataccat ctacgatgaa
      301 atgcttcatg tctttaataa tttgcgtcgg acggagagac aactgcgtca gatggagctg
      361 gagatgggtg aaaagtctgg tgaggatttg gataaactga tgtcagatta tgaccgctta
      421 tctgagaatt ttcgccaagc aggtggcttt acctatgaag ctgatattcg agcgattttg
      481 aatggattca agtttgacga gtctatgtgg cagatgaaaa ttgctgagct ttctggtggt
      541 caaaatactc gtttggcact tgccaaaatg ctccttgaaa agcccaatct cttggtcttg
      601 gacgagccaa ctaaccactt ggatattgaa accatcgcct ggctagagaa ttacttggta
      661 aactatagcg gtgccctcat tatcgtcagc cacgaccgtt atttcttgga caaggttgcg
      721 acaattacgc tagatttgac caagcattcc ttggatcgct atgtggggaa ttactctcgt
      781 tttgtcgaat tgaaggagca aaagctagtt actgaggcaa aaaactatga aaagcaacag
      841 aaggaaatcg ctgctctgga agactttgtc aatcgcaatc tagttcgtgc ttcaacgact
      901 aaacgtgctc aatctcgccg taaacaacta gaaaaaatgg agcgtttgga caagcctgaa
      961 gctggcaaga aagcagccaa catgaccttc cagtctgaaa aaacgtcggg caatgttgtt
     1021 ttgactgttg aaaatactgc tattggttat gacggggaag tcttgtcaca acctatcaac
     1081 ctagatcttc gtaagatgaa tgctgtcgct atcgttggtc caaatggtat cggcaagtca
     1141 acctttatca agtctattgt ggaccagatt ccttttatta agggagaaaa gcgctttggc
     1201 gctaatgttg aggttggtta ctatgaccaa acccaaagca agctgacacc aagtaatacg
     1261 gtgctggatg aactctggaa tgatttcaaa ctgacaccgg aagttgaaat ccgcaaccgt
     1321 cttggagcct tccttttctc aggagatgat gttaaaaaat cagtcggcat gctatctggt
     1381 ggcgaaaaag ctcgtttgct tttagctaaa ttgtctatgg aaaacaataa ctttttgatt
     1441 ctggatgagc cgaccaacca cttggatatt gatagtaagg aagtgctaga aaatgccttg
     1501 attgactttg atggtacctt gctttttgtc agtcatgatc gttactttat caatcgtgtg
     1561 gcaactcatg ttttggaatt gtctgagaat ggttcaactc tctaccttgg agattacgac
     1621 tactatgttg agaagaaagc aacagcagaa atgagtcaga ctgaggaagc ttcaactagc
     1681 aatcaagcaa aggaagcaag tccagtcaat gactatcagg cccagaaaga aagtcaaaaa
     1741 gaagttcgca aactcatgcg acaaatcgaa agtctagaag ctgaaattga agagctagaa
     1801 agtcaaagcc aagccatttc tgaacaaatg ttgggaacaa acgatgccga caaactcatg
     1861 gaattacagg ctgagctgga caaaatcagc catcgtcagg aagaagctat gcttgagtgg
     1921 gaagaattat cagagcaggt gtaaaaatgg aacatcttgg aaaagtattt cgtgaatttc
     1981 gaacaagtgg aaattattct ttaaaggaag cagcaggcga atcctgctct acctctcagt
     2041 tatctcgctt tgagcttggg gagtctgacc tggcagtctc ccgtttcttt gagattttgg
     2101 ataacattca tgtaacaatc gaaaatttca tggataaggc aaggaatttt cataatcatg
     2161 aacatgtgtc tatgatggca cagattatcc cactttacta ttcaaacgat attgcaggtt
     2221 ttcaaaagct tcaaagagaa caacttgaaa agtctaagag ttcgacgact cccctttatt
     2281 ttgagctgaa ctggattttg ctacaaggtc tgatttgtca aagagatgcg acttatgata
     2341 tgaagcagga tgatttggat aaggtagcag attatctctt caaaacagaa gaatggacca
     2401 tgtatgagtt gattcttttc ggtaacctct atagtttcta cgatgtagac tatgtcactc
     2461 ggattggtag agaagttatg gagagggagg aattttacca agagattagt cgccataaga
     2521 tattagtgtt gattttggcc ctcaattgtt accagcattg tttagagcat tcttcttttt
     2581 ataatgccaa ctattttgag gcttatacag agaagattat tgacaaaggt attaagcttt
     2641 atgagcgtaa tgttttccat tatttaaaag gttttgcctt atatcaaaaa ggacagtgta
     2701 aagaaggctg taagcagatg caagaggcca tgcatatttt tgatgtgtta ggtcttccag
     2761 agcaagtagc ctattatcag gaacactacg aaaaatttgt caaaagttaa ttttcccaaa
     2821 taagggaaaa aataaaaagc tcctttcggt tttgatacaa tagtttcaaa atttgagagg
     2881 agctttaata tgaatcgcta tgcagtgcag ttgattagcc gtggggctat caataaaatg
     2941 ggaaatatgc tctatgatta tggaaatagt gtctggttgg cttctatggg gactatagga
     3001 cagacagttt taggaatgta tcagatttct gagctcgtca catctattct cgtcaatccc
     3061 tttggcggag ttatttcaga ccgtttttct cgtcgtaaga ttttaatgac ggcagatctt
     3121 gtttgtggga ttctttgtct ggctatttct ttcataagga atgatagctg gatgattggc
     3181 gctttgattg ttgctaacat tgtgcaggct attgcttttg ccttttctcg cacagccaat
     3241 aaagctatca taactgaagt ggtggagaaa gatgagattg tgatctataa ttctcgctta
     3301 gagctggttt tgcaggttgt aggtgttagc tctcctgttc tttccttcct tgttttacag
     3361 tttgcaagtc tccatatgac gctactgcta gactcgctga cttttttcat tgcttttgtt
     3421 ctagtggctt tccttccaaa agaggaagca aaagttcaag agaaaaaggc ttttactggg
     3481 agagatattt ttgtagatat caaggatggg ttacactata tctggcatca gcaagaaatt
     3541 ttcttccttt tgctggtagc ttccagcgtt aatttctttt ttgcagcttt tgaatttcta
     3601 cttccctttt cgaatcagct ttactggtca gaaggagcct atgcaagtat tttaactatg
     3661 ggggctattg gttccatcat tggggctctt ctagctagta aaattaaagc taatatttat
     3721 aatcttttga ttttactggc tttgacaggt gtcggagttt ttatgatggg attaccactt
     3781 ccaacttttc tttccttttc tggaaattta gtttgtgaat tgtttatgac gatttttaat
     3841 attcactttt ttactcaagt acaaaccaag gttgagagcg aatttcttgg aagagtactg
     3901 agtacaattt ttaccttagc tattctattt atgcctattg caaaaggatt tatgacagtc
     3961 ttgccaagtg tacatctctc ttctttcctg ataattggaa gtggtgttat catcttgtct
     4021 tgtatatcgt ttatttatgt tcgaactcat tttgaaaaat t
//
LOCUS       DAVKJD010000110         3878 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKJD010000110 DAVKJD010000000
VERSION     DAVKJD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.110"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            16..>3878
                     /locus_tag="VW157_001882"
     CDS             16..>3878
                     /locus_tag="VW157_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9553135.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKKKTGVNTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTK
                     KAQVIKEQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKG
                     VPEVQPALPEAVMTDKGDPEQVEPLPEYTGVQAGAIVEPEKVEPEYAGVQAGAIVEPE
                     QVAPLPEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVE
                     PEKVEPSKEYTGVQAGAIVEPEQIAPLPEYTGVQAGAIVEPEKVEALKEYTGKIEQPS
                     AEDTKPNNENTNTPEEMSIQKKSSALINMNFITDSSKVTGVGSATFIAPNVLLTVAHN
                     FINNSTDNTTGEFRGDKSKNVYEWVTPDGQKGTFTANNIHFYNKKDYPKGFIYDLAVI
                     KLPETTGREHVELVKNYSKVNLNDKLNVHGYPAGKYTHLKDATVEMEQEYANNTYGVQ
                     YQGGNPGMSGGGIFNANGEVIGVHQNGAQNRSGGLILSPTQLAWIKSIIAGNEIPPVY
                     DELYRHKDEKKDDAKDEKEVIKKLELRNISSVELYSKDGNKYRHVTSLASLPSNAENY
                     FMKVKSENFKDVMLPVTSITNDTKDNRDVYKIVASANSLIQHENNNVLENYTYYLPKT
                     QQSETGVYTSFKNLVDAMNSNPNGTFRLGATMDAREVELPDGQESYVNNVFHGILVGT
                     NNEKYYAIYNLKKPLFGELNGATVEKLSLKDVNISAKDDTATLAKEANNNTHIDNVHA
                     DGAIAGERSIGGLVSQVNNSTISNSSYTGRITNTYKTVASYQIGGLVGKLSGPRGLID
                     KSFASIDLSSNATQGDQSIGGIVGAVENSALISNSYAEGNLNNVQRFANVGGVVGNLW
                     DPVGGLEKSGRLSNVLSDVNVTNGNAIAGYNFNGIKANGTYSNKNNKVVNVVQEDDEI
                     LTKDSTVQRGEVLEDAQIKEKKATFV"
BASE COUNT         1473 a          585 c          807 g         1013 t
ORIGIN      
        1 tttcaaggag gaataatgga aaagtatttt ggtgaaaaac aagagcgttt ttcatttaga
       61 aaattatcag taggacttgt atctgcaacg atttcaagtt tattttttat gtctgtatta
      121 gctagttcat ctgtggatgc tcaagaaact gcgggagttc actataaata tgtggcagat
      181 tcagagctat catcagaaga aaagaagcag cttgtctatg atattccgac atacgtggag
      241 aatgatgatg aaacttatta tcttgtttat aagttaaatt ctcaaaatca actggcggaa
      301 ttaccaaata ctggaagcaa gaatgagagg caagccctag ttgctggtgc tagcttagct
      361 gctctgggaa ttttaatttt tgctgtttcc aagaaaaagg ttaagaataa aacggtatta
      421 catttagtat tggttgcggg aatgggaaat ggtgtcttag tttcagtcca tgctttagaa
      481 aatcatcttt tgctaaatta caatacggac tatgaattga cctctggaga aaaattacct
      541 cttcctaaag agatttcagg ttacacttat attggatata tcaaagaggg aaaaacgact
      601 tctgattttg aagtaagtaa tcaagaaaaa tcagcagcca ctcctacaaa acaacaaaag
      661 gtggattata atgttacacc aaattttgta gaccatccat caacagtaca agctattcag
      721 gaacaaacac ctgtttcttc aactaagccg acagaagttc aagtagttga aaaacctttc
      781 tctactgaat taatcaatcc aagaaaagaa gagaaacaat cttcagattc tcaagaacaa
      841 ttagccgaac ataagaatct agaaacgaag aaagaggaga agatttctcc aaagaaaaag
      901 actggggtaa atacattaaa tccacaggat gaagtgctat caggtcagtt gaacaaaccg
      961 gaactcttat atcgtgacga aactatagaa acaaaaatag attttcaaga agaaattcaa
     1021 gaaaatcctg atttagcaga aggaactgta agagtaaaac aagaaggtgc attaggtaag
     1081 aaagttgaaa tcgtcagaat attttctgta aacaatgaag aagtttcacg agaaattatt
     1141 tcaacttcaa cgactgcgcc tgtttcaaga atagtcgaaa aaggtactaa aaaagctcaa
     1201 gttataaagg aacaagctga gactggtgca gaacataagg aagtacagtc tggagctatt
     1261 gttgaacccg caattcagcc tgaactacca gcagctgtat taaccgacaa aggtgagtca
     1321 gcagtccaac cagagttgcc agaagctgta gtaagtgata aaggcgtacc agaagttcaa
     1381 ccagcgttac cagaggcagt catgactgac aagggtgatc cagagcaggt agaaccgcta
     1441 ccagaatata caggagtaca agctggagct attgttgaac ctgaaaaagt tgaaccggaa
     1501 tatgctggag tacaagctgg cgctattgtt gaaccagagc aggtagcacc actaccagaa
     1561 tacacagggg tgcaagccgg agcgatagtc gagccagaga aagtagaagc accgaaggaa
     1621 tacacaggag tgcaggctgg agcgatagtc gaaccagaga aggtagaagc accgaaggaa
     1681 tacacaggag tgcaagccgg agcaatagta gaaccagaaa aagtagaacc atcaaaagaa
     1741 tacacagggg tgcaggctgg cgctattgtt gaaccagagc agatagcacc actaccagaa
     1801 tacacagggg tgcaagccgg agcgatagtc gagccagaga aagtagaagc actgaaagaa
     1861 tatactggta aaatcgaaca acctagtgca gaagatacta aaccaaataa tgaaaatact
     1921 aatacaccag aagaaatgag tattcagaaa aaatctagtg cattaattaa tatgaacttt
     1981 attacagatt cgtcgaaggt tactggagta ggatcagcta cttttattgc accaaatgta
     2041 cttttaacag tagcacataa tttcattaat aattctactg ataatactac tggcgaattt
     2101 agaggtgata aatctaaaaa cgtatatgag tgggtgactc ctgatggaca aaaaggaaca
     2161 tttactgcta acaatattca tttttataat aaaaaagatt atccaaaagg atttatctat
     2221 gatttagcag tgattaagct acctgaaaca actggcagag aacatgtaga attagttaaa
     2281 aattattcga aagtaaatct taatgacaaa ctaaatgttc atggatatcc agctgggaaa
     2341 tatacgcact taaaagatgc tacggtagaa atggaacaag aatatgcgaa taacacctac
     2401 ggtgttcaat atcaaggtgg aaatcctggt atgagtggtg gaggaatatt taatgctaat
     2461 ggtgaagtta ttggtgtgca tcaaaatgga gcacaaaatc gctcaggcgg attgatttta
     2521 tctcctacac aattagcttg gattaagagt attatagcag gtaatgaaat tccaccagta
     2581 tacgatgaac tttatcgtca taaagatgag aaaaaagatg acgctaaaga tgaaaaagaa
     2641 gttattaaga aattagaact tagaaatata tcttctgtag aactatattc aaaagatggt
     2701 aataagtatc gacatgtaac ttcacttgct tctttaccaa gtaatgcaga aaattacttt
     2761 atgaaagtta aatcagagaa ctttaaagat gtgatgctac cagttacaag cattactaat
     2821 gatactaaag ataatagaga tgtttataag atagtagcaa gtgcaaatag tctgatacaa
     2881 catgaaaata ataatgtact agaaaattat acatactatt taccaaaaac tcagcaaagt
     2941 gaaactgggg tatatacatc atttaaaaat ttagtagatg caatgaatag caatcctaat
     3001 ggcacattcc gtttaggagc aactatggat gctcgtgaag ttgaacttcc agatggtcaa
     3061 gaaagttatg tgaataatgt attccatgga atattagtag gaacaaataa tgagaaatat
     3121 tatgctattt ataatttgaa aaaaccatta tttggggaat taaatggtgc tacagtagaa
     3181 aaactttcac taaaagatgt taatatttcc gcaaaggacg atactgcaac acttgcgaaa
     3241 gaagcaaata acaatactca tattgataac gttcatgctg atggagcgat agctggggaa
     3301 cgtagcatag gaggattagt atcacaagtt aataattcta ctatttctaa cagtagctat
     3361 acaggtagaa taactaatac atataaaaca gttgctagtt atcaaatcgg aggtttagta
     3421 ggcaaacttt ctggtccgag gggattaata gataagtcgt tcgcatcaat cgatttatct
     3481 tcaaatgcaa cacaaggaga ccagtctatt ggtggaatag ttggtgcagt agaaaatagt
     3541 gcgcttatta gtaatagtta tgctgaagga aatctaaata atgttcaacg ttttgcaaac
     3601 gttggtggag tagttggtaa tctttgggat ccggttggag gattagaaaa atccggtcga
     3661 ctttctaatg tactaagcga tgttaatgta acgaatggta atgctattgc aggttataat
     3721 tttaatggta taaaagcaaa tggcacatac agtaataaaa ataataaggt tgtgaacgta
     3781 gtacaagaag atgatgaaat cttaactaag gattctactg ttcaaagagg agaagtatta
     3841 gaagatgcac aaataaaaga gaaaaaagct acttttgt
//
LOCUS       DAVKJD010000111         3647 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKJD010000111 DAVKJD010000000
VERSION     DAVKJD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.111"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(82..723)
                     /locus_tag="VW157_001883"
     CDS             complement(82..723)
                     /locus_tag="VW157_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9553136.1"
                     /translation="MIELENITKTIGGKVILDRLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLSKKDKLKKVEEILERVGLSATLHQKVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            complement(720..2828)
                     /locus_tag="VW157_001884"
     CDS             complement(720..2828)
                     /locus_tag="VW157_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9553137.1"
                     /translation="MKKISHLCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVITFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSLPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     ALASRMEKRSLVELLKGGIL"
     gene            complement(2883..3176)
                     /locus_tag="VW157_001885"
     CDS             complement(2883..3176)
                     /locus_tag="VW157_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEU9553138.1"
                     /translation="MIGEINHEKIGVASLMVCSILASTSVLAVWVDGGQWNYGVGWSG
                     NFGYSDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
     gene            3558..3647
                     /locus_tag="VW157_001886"
                     /pseudo
     CDS             3558..3647
                     /locus_tag="VW157_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         1183 a          796 c          585 g         1083 t
ORIGIN      
        1 aaatgtcatt cctaggcgta tttatgctat tcctctctgg aaaatatgag tattcagtca
       61 gtcaaaagaa gctgaacgaa ctcattttcc ctcgcccaat tcaataatcc gatgacattg
      121 ttgggctaca taagcatcgt gggtcacaat aatgactgtt ttcccttctc gattcatctc
      181 taagagaaac ttcaagacca aatctctatt ttcaggatcc agcgaacctg taggttcatc
      241 ggctaaaatc agctggctgg gttttaagat ggctctagca actgcaattc gttgttgttc
      301 gccaccagac aactcggaga ccttttgatg caaagtagct gacaaaccta ctctctctaa
      361 aatctcttcc acctttttga gcttatcttt cttagacaat tttacatatt tcagcgccag
      421 cataagattg tactcgaccg tttcatcatc aatcagggca aaattttgaa acagataaga
      481 gatatgttca cggattattg tttgcgactt agcagaattg accgctagat ttgtctgacc
      541 aaaaatctca taccgtccgc tataatcacc atctatcaaa cccaataaat ttaacaaggt
      601 cgacttccca ctaccactct taccaacaat ggcaaccaaa tccccctgat caatcctgag
      661 agacaagcga tccaaaatca ctttcccccc aatggttttg gtaatatttt ccaactcaat
      721 cataagatgc cccctttcaa taactctact agacttcttt tctccatcct agaagccaag
      781 gctagcacaa atagtatatc caaacatgta aagcctgcaa acagcaggag tggcaagaac
      841 gcatgggcaa agaaaatcaa gactagaaga gggagactat agcccagcaa gagcagaacg
      901 aggagaggac ggtagcgatc gaccagtttc caccccataa acttcttggt aataatatcc
      961 ctgcgcttca ataagaaagt tattactagt aagaagtagg aaatcatcat gctaaggaga
     1021 ccaaacaaag caaagagtag gttaaaattc cgaacagcat ctcgataaga atccactttc
     1081 tcttgttgaa tggcttgaat agatgaaaat tttaaataat ttccatctga caatttctca
     1141 actaactctg taatctcttt ttgatgttga accgtatttt caattttaat cggattattt
     1201 aagccagttg ttgacaggga ggctttctca tcccacatca tatcagaatc attgaccaag
     1261 ctaataattg gattggagag attttccttt cgcttatcac tatatgggaa aaatgaccaa
     1321 tctccttcat aataggcaat ctcgacatcc atctcctcta tcgttcgttt ttgctgctct
     1381 tcatacttca tcgaatgaaa ggcaattaac ttccccaaga gctgattttt atcttcttca
     1441 cctttcgtac ttgctggcat caaaataact tttttaatac cggtatctgg tagcttgaat
     1501 cccttgctct ttagaaaatt gcgattggca tagtaaacat ccaccgtatc tgttaactga
     1561 tattgctgaa tctgttctga ttggacaaaa ttttttacag gaagactgct actctgcaca
     1621 tagcccgcct gcgttttttc taccaaatcc tgataaaatc gatagaaata atctgtagat
     1681 ttccctgacc ctgctagctc ttcttgccac agattatcat tgagtttgaa ggtttctaag
     1741 gtcaggtaat taccttgact tacccactgt tgctgataag caagttcttt gttttcttgt
     1801 tctaaacttc tgcccacccc aatcagtaag gccgtcagta aaatagttgt ccctattttc
     1861 atcacataat tgaagataag accaaatttg aaagatgaaa aacctttcag cagagagctg
     1921 attgtcattt tttggattaa gaggtaagtc aaccaactga taaagagata aagctgcaac
     1981 agcaaaaaat gagacaacca cagcatagga aacaaatctt ttggcttata atcaagcaag
     2041 aaaaacacgc ctagattgat cacaagagcc ccacctagga ggaggtaaag gttgcctttt
     2101 acaacatcag ctaaaacagc cctatcttga aaaccaagta atttttgtac cccaactctt
     2161 ttcatctcca tcatcggttg atacactgtc actaacacaa gaagcaaaat agccaagaca
     2221 aaaacaatgg cagataaaag caaatctcga tttatgactt ccactgcact tttgtaggtc
     2281 ggctctagca aggtagcctg gtctatcttg aaaaaatcgc tccatttctg tacaatccta
     2341 tccttgtcca tctcttgtgt agaagttatc gtatagcgac catttaaact acgagatgta
     2401 tccttgatat aggtttgaaa agtcataagc tgaataggtt tggcttttag aaaggtcgga
     2461 atcgtaccaa gtttattgga aatttcttta ttactataga ctccttcacc atctgtggta
     2521 aaatcaagag aagaaatccc aaactcttgg taggggaagg tatctttatc aaaaacacca
     2581 gacttgacca cctcatcacc actgtctgtt ttgatgatgg agactttata ctcctttgat
     2641 acatcctcaa aaaatcgaag aacagacgct gcaggttcgt taatatcttt caaatacaaa
     2701 tccaaagaat ctacagtctt tcccatttct tgaatccgaa ccacttctcg tgtatagttt
     2761 acattaaaaa caaaaaaagt ggtacacaga agcaggagta acatacaaag atgactgatt
     2821 tttttcatag agatacctcc cgtgaagaaa ctccctctcc aaaaggggag ggagaatttt
     2881 aattaaaatc catagaaata ttccattccc gttggtggga atttgaagat tgaagcccga
     2941 gcccatgcct cagcttctgc acgatcctta gagaatttat ttccatcttt aacagttgct
     3001 gtatgagagc gagtagaatg taaataatca gaataaccaa aatttcctga ccatcctact
     3061 ccatagttcc attgaccacc atcaacccat actgccaaaa cacttgtaga agcaagtata
     3121 ctacatacca ttaaagatgc aacaccaatt ttctcatgat ttatttctcc tattatcgag
     3181 agctattccg tagaaacagc tatagtaatt tgtcaacaag tatcgtagtc cattggactc
     3241 accttccttc tatctcattt catttttaaa caacgaactt actcttaact agaagaatag
     3301 ttctcttctg tatcaggtct ttgaacaacc agagtagtat taatcacact ctaactatta
     3361 ctcaagaatc agcccaataa acaatatgga tagcgctttc aaatttttaa actcttatcc
     3421 catcctttta tctatataat aagtgaaaat ataataactg tcaagtaatc gaagtgaatt
     3481 ttataaaaaa aattacaagc caaatttgca aagtttacaa taagccgcta gggcaatcgt
     3541 ctatcagaat gtccgtttat ttgtcaataa tccgagaaaa tcttgcaacg cttagaagtc
     3601 tataaaaact atcaacattt atatgacttg cgaatagcaa tcctgct
//
LOCUS       DAVKJD010000112         3633 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKJD010000112 DAVKJD010000000
VERSION     DAVKJD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.112"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..101
                     /locus_tag="VW157_001887"
     CDS             <1..101
                     /locus_tag="VW157_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9553139.1"
                     /translation="TYDLEPMAQQVIPKAAFGYIASGAGDTFTSFQ"
     gene            229..1719
                     /gene="lysS"
                     /locus_tag="VW157_001888"
     CDS             229..1719
                     /gene="lysS"
                     /locus_tag="VW157_001888"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9553140.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            2344..3480
                     /gene="lctO"
                     /locus_tag="VW157_001889"
     CDS             2344..3480
                     /gene="lctO"
                     /locus_tag="VW157_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9553141.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
BASE COUNT         1105 a          713 c          777 g         1038 t
ORIGIN      
        1 atacctatga tttggagcca atggcgcaac aagttattcc taaagcagca tttggctata
       61 tcgctagtgg ggcgggagat actttcactt ctttccagtg attttagcgt caggttcttt
      121 ttagttttta aagattatcc gtgaatttct tgcttattta tgataaaatg ggagtatcgc
      181 aaaaaatgac tcatcgtatt caattttgag taaaactagg aggatcccat gtctacagaa
      241 catatggaag aactaaatga ccagcagatc gttcgccgtg aaaaaatggc tgcgctccgc
      301 gaacaaggaa tcgatccttt cggaaaacgt tttgaacgta ctgcaaattc acaagaatta
      361 aaagataaat atgccaacct cgataaagaa caattacacg ataaaaacga aacagctact
      421 atcgcaggac gcttgataac caaacgtggt aaaggaaaag ttggttttgc ccaccttcaa
      481 gaccgcgaag gccagattca gatctacgtt cgtaaggatg ctgtcggtga agaaaactac
      541 gaaatcttca aaaaagcaga tcttggtgac ttccttggtg tcgaaggtga agtgatgcgt
      601 acggatatgg gagaactctc tatcaaggca acccacatca cacacttgtc taaggctctt
      661 cgtcctcttc ctgagaaatt ccatggtttg acagacgttg aaacaattta ccgtaaacgt
      721 taccttgact tgatttctaa tcgtgaaagc tttgaacgtt ttgtcactcg ttcaaaaatc
      781 atctctgaaa tccgtcgtta ccttgaccaa aaaggattcc ttgaagtgga aacacctgtt
      841 cttcacaacg aagctggtgg tgctgctgcc cgtccattta tcacccacca caatgcccaa
      901 aacattgaca tggtgcttcg tatcgcgact gaacttcact taaaacgcct tatcgtgggt
      961 ggtatggaac gtgtctatga aattggccgt atcttccgta acgaaggaat ggacgctact
     1021 cataaccctg agtttacttc tatcgaagtt taccaagctt atgcagactt ccaagacatc
     1081 atggacttga cggaaggcat tatccaacac gctgctaaat cagttaaggg cgatggccca
     1141 gtcaactacc aaggtactga aatcaagatt aacgaaccat ttaagcgtgt tcatatggtg
     1201 gatgctatca gagaaattac tggtgtcgat ttctggcaag acatgacttt agaagaagct
     1261 aaagctatcg ctgctgagaa gaaagttcca gttgagaaac actacactga ggttggtcac
     1321 atcatcaatg ccttctttga agagtttgtt gaagaaactt taatccaacc aacctttgtc
     1381 tatggacatc cagtagctgt atctccactc gctaagaaaa atcctgaaga ccaacgcttt
     1441 actgaccgtt tcgagctctt tatcatgact aaggagtacg gtaatgcctt tactgaactc
     1501 aacgacccaa tcgaccaact tagccgtttt gaagcccaag ctaaagccaa agaacttggt
     1561 gatgatgaag cgacaggaat cgactatgac tacattgaag ctcttgaata cggtatgcca
     1621 ccaacaggtg gtttgggaat cggtatcgac cgtctctgca tgctcctcac tgatacaaca
     1681 actatccgcg atgtattgct cttcccaaca atgaaataaa ttcttatcct ctggctctta
     1741 ccagaggatt ttttgattca aaaagagact gaatttaagg agaaaatgaa gtgtagtata
     1801 ttgaagttga aatagtacac tttgatttct aagacattgt tagaaattgg tttaaattcc
     1861 ctaatcaatt tgtgcatgtt ttatttcatt ttacgatagt acgctgaaac tgttcaaaaa
     1921 gtactagaaa ttgacttgga ttccccaatt gatttgttca gattcactat aaataaaaaa
     1981 ttaataagtg ggataggaag ttagcgtcaa ctaggatagt atcttgctta aacagtatat
     2041 atgggactga tataagtcca taggtcctat tagaggatgt tctggtgtct tattcacttg
     2101 cttttttata gtattagtag atagaatcag caaataaaaa agtagttcaa atgcctggca
     2161 gcaaaaaatc tcaacgattc acttaatata gactaaagtt tagagtttaa ggaaagtagg
     2221 gctagtatat tgtatgaaaa aataagatgg tttttaagca aaactcttga aaatgattga
     2281 aaaaagagtt attatagttg tgtagtaaaa agcaaacgct tacttaatca aggagggcat
     2341 tttatgtcat acaaaacaag caatgcagaa ggtcatgtag acttcatcaa tacctatgat
     2401 ttggagccaa tggcgcaaca agttattcct aaagcagcat ttggctatat cgctagtggg
     2461 gcggaagata ctttcacttt aagagagaat atccgcgcct ttaaccacaa actcatcgtt
     2521 cctcatacac tttgcgatgt tgaaaatcca agtacagaga ttgaatttgc aggtgaaaaa
     2581 ctatcttcac caatcattat ggcgcctgtt gcggctcata aattggcaaa tgaacagggg
     2641 gaagtggcga ctgcgcgtgg tgtgcatgag tttggttctc tttatacaac cagctcttac
     2701 tctactgttg accttccaga gatttctgaa gcccttcaag ggacacccca ttggttccaa
     2761 ttttacttta gtaaggatga cggtatcaac cgccacatca tggaccgtgt gaaggctgaa
     2821 ggttataaag cgattgtctt gacggcagat gctactgtag gggggaatcg tgaagtggat
     2881 aagcgtaatg gttttgtctt cccagttggc atgccgattg ttgaagaata cctgccagaa
     2941 ggtgctggta aatcaatgga ctttgtttac aaatcagcta aacaacgctt gtctccacgc
     3001 gatgtagaat ttatcgctga atactctggt cttcctgtgt atgtcaaggg accacaatgc
     3061 cgtgaggacg ttgaacgttc acttgctgca ggagcttctg gtatctgggt aaccaaccac
     3121 ggtggtcgtc aaatcgacgg tggaccagct gcctttgact cgcttcaaga agtggcagaa
     3181 gcagttgata gacgtgtgcc gattgtcttt gactctggtg ttcgtcgtgg tcaacacgtc
     3241 tttaaagcct tggcttcagg agcagacttg gtagctattg gtcgccctgt tatttatggc
     3301 ttggctctcg gtggtagtgt cggtgtgcgt caagtctttg aacacttgaa tgcggaattg
     3361 aagacagtta tgcaattgtc tggagctcag accattgaag atgtcaaaca ctttaagctc
     3421 cgtcacaatc catacaaccc aactttccca gttgaccctc gtgacttaaa attgtattga
     3481 taaaacagat tgcctccact gaatgtggag gtttttcttc gtgtattgga agaataaatt
     3541 gctatcgaaa actatagtaa aatgaaataa gaataggaca aatcaatcag gacagtcaaa
     3601 tcgatttcta acaatgtttt agaagtagag gtg
//
LOCUS       DAVKJD010000113         3477 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKJD010000113 DAVKJD010000000
VERSION     DAVKJD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.113"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..172
                     /locus_tag="VW157_001890"
     CDS             <1..172
                     /locus_tag="VW157_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9553142.1"
                     /translation="RTVSFAISSKGEKMKVENISYRVDHRILFDNISFATSSSGVTLI
                     TGKNGTGKSTLL"
     gene            complement(523..1833)
                     /locus_tag="VW157_001891"
     CDS             complement(523..1833)
                     /locus_tag="VW157_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9553143.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2070..2942
                     /locus_tag="VW157_001892"
     CDS             2070..2942
                     /locus_tag="VW157_001892"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9553144.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3073..3469
                     /locus_tag="VW157_001893"
                     /pseudo
     CDS             3073..3469
                     /locus_tag="VW157_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1095 a          592 c          772 g         1018 t
ORIGIN      
        1 cagaactgtt tcttttgcta ttagttcaaa aggagaaaaa atgaaagtag aaaatatttc
       61 gtatagggtg gatcatcgta tattgtttga taatatttct tttgctactt cgagttcagg
      121 cgtgacatta attactggta aaaatggtac aggaaagtca actttactat agtagattga
      181 aactagaata gtacacctct acttctaaaa tgttgttaga aatcgatttg actgtcctgt
      241 tcttatttca ttttactaca tctcaaattg agtatgacga agtgcgctcc catgtcatgg
      301 gaacgcactt cgtcatattt ttcatattct tgaatccatc gataaagact attgggatga
      361 atttttaaag ttgaactaat catttttaca ggatgaggtt tacagcagag agtttgaagg
      421 ctttatcaaa ggtttttctt ggcataatga cttttcctcg tttccactta attttgtgtc
      481 tactttatta taccaagtcc acccttaagt tagataataa atctaactta aggaagctag
      541 aaggatgaga atccaggtgg tcaagagtcc caaacttaag ctgatgggga cacccagaat
      601 aatttgcttt ttgaaggcaa ggccacgttc ctctatattg ggaagtgaga gttgaatgag
      661 agaaccagct gatgaaaagg gtgagatatt agtagataga gcgccaataa cggtggctgt
      721 tgtgagtaag tgaatatcaa tctgaggatt ttgagcactg atgatagcaa tgatgggaaa
      781 gagggctgga gctacaacgg atagggtgga actaaagagt gacatcactc cggctatcac
      841 acaaaagaac agaggtaacc agaaatgagg aatggttgtt gtcatgaggt gccctatcag
      901 tgtgactaaa cctgacttga ccgctagaga cattagtaag ctcatgccgc agagcatgat
      961 aattgtagcc cagggaacct tagccaaaat ggcttcttgc ttccctaatt tgagccttaa
     1021 ggcgaggcag accatgagta ttgagacaaa gccaatatca aatgtttttc gataagtagc
     1081 tatccaggcg atgtttggga aaatgagatg caacaaggga aaaagccaga ccaaaaccat
     1141 gctgctgatc atgagcaagg tggtttgtct ttgaaccttg ctgaggagtg gtggttggtc
     1201 aatagtcaag gatgagtttg ttcttccctt actatagtgg ctgtaacagg ataataaaag
     1261 caagacgatg agtgggtaga taatgctgac gataaagata tgattgccaa gtgaaaaagc
     1321 ttgctcttcc catcccattt gcttaaacag gccttgaaag acaatgcctg agctactggt
     1381 tatcaaatta gcccctcctg aagctcccca attgacggct tgagctccaa tcaaagggtg
     1441 tttgtccgct ttttgacaga gggtaatcgc tagaggacag caaacggcca tagtagtgaa
     1501 aaatccagca cctaaagcag acaaaagggt tgccatcagg tataaaatca tgtagagggc
     1561 gttagggtgg gtgcgtgtgc ggtagagaat gtgttgagcc aaaacatcaa gagtaccgtt
     1621 agttgttgca acgttataaa agagagagac gctaaaaatg gtaaaaaaga gtgaggttgg
     1681 ccaaaaatga agaagttctt tggggcttaa tcccatgaga gtggttgcga tgaggtaaga
     1741 aaaagcaata gccagcaggc caatattgat tttggtgcgg taaccaattc caatggctag
     1801 agcaatggcg ctaatcatta ttaaatgaat cattgaattg tcctttcagt tagaatatgg
     1861 aaatagattg gcaattttgg cgctcaaaag accacctgtt tcctattggt acttgactat
     1921 tatacacaaa aaaaatacaa ttgtcttgac aattgtattg acacttgtta gaattaaaaa
     1981 tgaggtattc aggcttgtga tttcctatgg aagttaatag taattgcctc tagtgcttac
     2041 aagtgatatt aaaaatagag gacttagtga tgtcaatcat ttcaactgat ttaacccctt
     2101 ttcaaataga tgatacattg aaagcagcct tgcgagaaga tgttcattcc gaagattaca
     2161 gtaccaatgc catttttgat catcatggcc aagccaaggt gtcgcttttt gccaaggaag
     2221 ctggtgtttt agcgggacta accgtttttc aaagggtttt taccctattt gatgccgagg
     2281 tgaccttcca gaatcctcat caatttaagg atggggatcg tttgactagt ggcgatttgg
     2341 ttttagaaat cataggctcg gtgagaagtc tcttaacatg tgaacgcgtt gccttgaatt
     2401 ttttacaaca tttatcaggg attgcttcga tgacagctgc ttatgtagaa gccttaggcg
     2461 atgattgcat taaggtattt gatactcgaa aaactactcc taatttacgt ctttttgaga
     2521 aatatgccgt gagagttggc ggtggctata atcatcgctt taatttatca gatgctatca
     2581 tgctaaaaga caatcacatt gcggcagtag gtagtgttca aaaggcaatt gctcaagcgc
     2641 gtgcctatgc cccttttgtg aaaatggtcg aggtggaagt ggaaagcctt gctgctgccg
     2701 aagaagctgc ggcggcgggt gctgatatta tcatgttgga taatatgtca ttggaacaga
     2761 ttgaacaggc tattacccta attgcaggac gttctcggat tgaatgttct ggaaatattg
     2821 atatgaccac tattagccgt tttcgtggtt tagcgattga ttacgtctcc agtggtagtt
     2881 taacccatag tgctaagagt cttgattttt ccatgaaggg gttaacctac cttgatgtct
     2941 aagttgtaaa ataaactaac tttttaaagg atgtttttcc tctagaacga gttttatgtc
     3001 agatagttta aacgcctctt caaatatagt aaaatgaacc aaaaatagta cacaatgtgg
     3061 tataatcttt ttatggcata ttcaatagat tttcgtaaaa aagttctctc ttattgtgag
     3121 caaacaggta gtgtaacaga agcataacac gttttccaaa tctcacgtaa taccatttat
     3181 ggctggttaa agctaaaaga gaaaacagga gagctaaacc accaagtaaa aggaacaaaa
     3241 ccaagaaaag ttgatagaga tagacttaaa aactatctta ctgacaatcc agatgcttat
     3301 ttgattgaaa tagcttctga atttggctgt catccaacta ccatccacta tgcgctcaaa
     3361 gctatgggct acactcgaaa aaaagaacca cacctactat gaacaagagc cagaaaaagt
     3421 agccttattt cttaagaatt ttaatagttt aaagcaccta gcacctgttt agattga
//
LOCUS       DAVKJD010000114         3317 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKJD010000114 DAVKJD010000000
VERSION     DAVKJD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.114"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(136..747)
                     /gene="rpsD"
                     /locus_tag="VW157_001894"
     CDS             complement(136..747)
                     /gene="rpsD"
                     /locus_tag="VW157_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9553145.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(942..1994)
                     /locus_tag="VW157_001895"
     CDS             complement(942..1994)
                     /locus_tag="VW157_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9553146.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(1991..2689)
                     /locus_tag="VW157_001896"
     CDS             complement(1991..2689)
                     /locus_tag="VW157_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9553147.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2856..>3317)
                     /locus_tag="VW157_001897"
     CDS             complement(2856..>3317)
                     /locus_tag="VW157_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEU9553148.1"
                     /translation="QALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKI
                     NVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASA
                     NKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT          915 a          748 c          647 g         1007 t
ORIGIN      
        1 actttggact atatcggtct agttatgtct aaacgattat agctggatac taagagaaat
       61 caaaaaagca ctaagggaga acgtcccaaa gtgcttaata tcaaggcctc aaagcctatc
      121 ttattcaata aaatattaca acattttgtt gtagaattca acgacaagtg cttcgttgat
      181 ttctgggttg atttcgtcgc gttctggcaa gcgagtcaat gaaccttcca atttttcagc
      241 gtcgaatgat acgaatgctg gacgtccaag agtagcttct actgcttcaa ggattgctgg
      301 aactttcaat gatttttcac gaactgagat cacttgacct ggagttacgc ggtatgatgg
      361 gatatcaacg cgtttcccgt caacaaggat gtgaccgtgg tttacgaatt gacgagcttg
      421 acgacgagta gtcgcgagac caagacggta aacaacgtta tccaaacgac gttccaaaag
      481 aagcataaag ttgaaaccta ggattccgcc tttgattttt gtagcttgta cgaacaagtt
      541 acggaattgt ttttcaccta caccgtaagt gaaacgaagt ttttgttttt cagccaattg
      601 caaaccgtat tctgacaatt tagaacggtt gtttggtccg tgttgtcctg gtacgtagtt
      661 acgacgtgcc aattctttac ctgtacctgt aagtgaaagg ccaaggcgac gagcttgttt
      721 ccaagatggt cctgtataac gtgacatatg aatgtcctcc tgatataaat aatatttcgg
      781 tggaaatagt cacttagaaa gccctgattc gtgcagatgc ccttcgccta aacagccaag
      841 gttacttatc ataagacacc tgttgacgag cttcatgctt tcctgctgat atttcacaca
      901 aggtccattt taccatgaat agctggattt gtaaaggggt tttaggcttt attttcacta
      961 ccagagaggt tgagaacgag ggtaaaggta cttcctagac cgtactggct gctgactgtg
     1021 atttccccac ccaattgatg ggccaattca cgcgcaatcg caagtcctaa tccatgacca
     1081 cctgtcttca tgttacgcga agtttcgaca cgataaaggc gtttgaaaat attttccaaa
     1141 tcctctgggg caatgccctg cccttcatcg gtcacactga ttgaaagctg gcccttctcc
     1201 agcttagcca ccacttccag cttggttcct ggagcagaat atttaaaagc gttatcgacc
     1261 agattcacca agatacgaga aagcttagca taatctccct caatccgggc agactctggg
     1321 attacctgca agtggacatc tcttctctcc tgctcaatca aaaactgaaa ttcactcatg
     1381 cactcaatta agagcttgtc cagaaaaata ctgtctttac tggtagtttc cacctgattt
     1441 ctagctgtgt ttagggtcaa aaaattcaac tcctcaacca gtttattgag cctctccgtc
     1501 tggcgtccaa tggttgctag ataatgagct tgctccgact ccttgataat cccatccaaa
     1561 atcccttcta ccgtcgcttg gatcgaagtg ataggagtct taatatcatg cgacaactgg
     1621 gcaatcatca agcccttttc tcgttcgctt tcttccaagg aatcaaagct tacctgcaaa
     1681 tcatgggaca tctcattaaa agtttgccct aattgctgaa attctacagg accttgaacc
     1741 tccaaatttg aaggaaaatc cttggccgct acccgcttgg catgctcctt gagtttgccc
     1801 aacgacgtaa agactggcaa taggagaaag agactaatcc cagcaccgac aaggctggca
     1861 acgatggtca tcccaagcaa aaagtaaatc tcgcctttcg caatcagcat tttttgaaca
     1921 gcccaaaaaa caaccaaaat ggttaagagg gttgaaataa tatatccaac caaaatataa
     1981 ctttttagtt tcatgtttgt cctctcggtt tctctatctt atatcccaac ccccaaactg
     2041 tcttaatagt gggcgtttgg tcactactat attttgccag ctcctgtcga agagcatgga
     2101 tatgcacatt caaggtattg gtgtcatcca cgtagtcttc tttccagatc ttttcataga
     2161 ggtctgtctt ggagaaaact cgctctggat tgctagctaa aatccacagc aattcaaatg
     2221 atttaacagt taaatccagc atttcttctc ctatttgaac ttcatgacta ctatgattca
     2281 tttttagatt gccaagggaa atcagctctg tttcgccccc acgatgaagg cggcgcaaaa
     2341 tattgtggac acgcaaaacc agctcacgtg ggctaaaagg cttagcaata aaatcatctg
     2401 ctcccaagct caggccgtaa atcttgtcct gttcactggt cttagcagta ataaatagga
     2461 aaggctgctc tggtgataag tattgaacct cactgattaa atcataacca tccatccgag
     2521 gcatcatgac atctgtgata atcaaatcaa tcggtttttt cttgaacagc tctagcgctt
     2581 ccagtccatc atgggctacc aagacctgat aacctgcctg aattaagtat ctctgatgaa
     2641 tatctgtgat ttctacctcg tcgtcaacga gtaaaattgt ctttcccatc tgtctctcct
     2701 ttgataaaaa cagtgctata ctgctttaag tataacacta tttttattgt tttacgatga
     2761 ttttaatctg ctattgaaag aaagtcctga ttgctgtttg agttgttaaa ctaatcttag
     2821 tcagcccact atccatcatt tatttcctta gctgattagt tttcttcttt tttgtttttc
     2881 aaacctagac caccgagtaa acctgcaagc gcaagcccaa ggaaaccaat acttgccatt
     2941 gatgtttgag tctcaccagt atttggtagc atagctttat cctctgacat catcgtatca
     3001 gacatcttgt tagcagaagc agccatgttt tcacctgcca tcgtgttggt agaacttgtc
     3061 atggtgtcag caggcatgct atctgtagta cctgtagcat gattgtgatt catcggagtc
     3121 acgccagaac cagagttaga aggtgataat gaaccatttg ctgtgtctga tgtttcttta
     3181 acatttatct taatagtgac ttttttagtt gctacgatgt tgtccaaatc tggtttaccg
     3241 tctttgttac cataaacatt gactgtagcg ctgtaagttt gagtaccgtt tgctcggaac
     3301 tggtcaatga gcgcttg
//
LOCUS       DAVKJD010000115         3282 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKJD010000115 DAVKJD010000000
VERSION     DAVKJD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.115"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            32..3151
                     /gene="phtE"
                     /locus_tag="VW157_001898"
     CDS             32..3151
                     /gene="phtE"
                     /locus_tag="VW157_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU9553149.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQFGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENRKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1222 a          546 c          634 g          880 t
ORIGIN      
        1 aaacttcatc atttattgaa atgagggatt tatgaaattc agtaaaaaat atatagcagc
       61 tggatcagct gttatcgtat ccttgagtct atgtgcctat gcactaaacc agcatcgttc
      121 gcaggaaaat aaggacaata atcgtgtctc ttatgtagat ggcagccagt caagtcagaa
      181 aagtgaaaac ttgacaccag accaggttag ccagaaagaa ggaattcagg ccgagcaaat
      241 tgtaatcaaa attacagatc agggctatgt aacgtcacac ggtgaccact atcattacta
      301 taatgggaaa gttccttatg atgccctctt tagtgaagaa ctcttgatga aggatccaaa
      361 ctatcaactt aaagacgctg atattgtcaa tgaagtcaag ggtggttata tcatcaaggt
      421 cgatggaaaa tattatgtct acctgaaaga tgcagctcat gctgataatg ttcgaactaa
      481 agatgaaatc aatcgtcaaa aacaagaaca tgtcaaagat aatgagaagg ttaactctaa
      541 tgttgctgta gcaaggtctc agggacgata tacgacaaat gatggttatg tctttaatcc
      601 agctgatatt atcgaagata cgggtaatgc ttatatcgtt cctcatggag gtcactatca
      661 ctacattccc aaaagcgatt tatctgctag tgaattagca gcagctaaag cacatctggc
      721 tggaaaaaat atgcaaccga gtcagttaag ctattcttca acagctagtg acaataacac
      781 gcaatctgta gcaaaaggat caactagcaa gccagcaaat aaatctgaaa atctccagag
      841 tcttttgaag gaactctatg attcacctag cgcccaacgt tacagtgaat cagatggcct
      901 ggtctttgac cctgctaaga ttatcagtcg tacaccaaat ggagttgcga ttccgcatgg
      961 cgaccattac cactttattc cttacagcaa gctttctgcc ttagaagaaa agattgccag
     1021 aatggtgcct atcagtggaa ctggttctac agtttctaca aatgcaaaac ctaatgaagt
     1081 agtgtctagt ctaggcagtc tttcaagcaa tccttcttct ttaacgacaa gtaaggagct
     1141 ctcttcagca tctgatggtt atatttttaa tccaaaagat atcgttgaag aaacggctac
     1201 agcttatatt gtaagacatg gtgatcattt ccattacatt ccaaaatcaa atcaatttgg
     1261 gcaaccgact cttccaaaca atagtctagc aacaccttct ccatctcttc caatcaatcc
     1321 aggaacttca catgagaaac atgaagaaga tggatacgga tttgatgcta atcgtattat
     1381 cgctgaagat gaatcaggtt ttgtcatgag tcacggagac cacaatcatt atttcttcaa
     1441 gaaggacttg acagaagagc aaattaaggc tgcgcaaaaa catttagagg aagttaaaac
     1501 tagtcataat ggattagatt ctttgtcatc tcatgaacag gattatccaa gtaatgccaa
     1561 agaaatgaaa gatttagata aaaaaatcga agaaaaaatt gctggcatta tgaaacaata
     1621 tggtgtcaaa cgtgaaagta ttgtcgtgaa taaagaaaaa aatgcgatta tttatccgca
     1681 tggagatcac catcatgcag atccgattga tgaacataaa ccggttggaa ttggtcattc
     1741 tcacagtaac tatgaactgt ttaaacccga agaaggagtt gctaaaaaag aagggaataa
     1801 agtttatact ggagaagaat taacgaatgt tgttaatttg ttaaaaaata gtacgtttaa
     1861 taatcaaaac tttactctag ccaatggtca aaaacgcgtt tcttttagtt ttccgcctga
     1921 attggagaaa aaattaggta tcaatatgct agtaaaatta ataacaccag atggaaaagt
     1981 attggagaaa gtatctggta aagtatttgg agaaggagta gggaatattg caaactttga
     2041 attagatcaa ccttatttac caggacaaac atttaagtat actatcgctt caaaagatta
     2101 tccagaagta agttatgatg gtacatttac agttccaacc tctttagctt acaaaatggc
     2161 cagtcaaacg attttctatc ctttccatgc aggggatact tatttaagag tgaaccctca
     2221 atttgcagtg cctaaaggaa ctgatgcttt agtcagagtg tttgatgaat ttcatggaaa
     2281 tgcttattta gaaaataact ataaagttgg tgaaatcaaa ttaccgattc cgaaattaaa
     2341 ccaaggaaca accagaacgg ccggaaataa aattcctgta accttcatgg caaatgctta
     2401 tttggacaat caatcgactt atattgtgga agtacctatc ttggaaaaag aaaatcaaac
     2461 tgataaacca agtattctac cacaatttaa aaggaataaa gcacaagaaa actcaaaact
     2521 tgatgaaaag gtagaagaac caaagactag tgagaaggta gaaaaagaaa aactttctga
     2581 aactgggaat agtactagta attcaacgtt agaagaagtt cctacagtgg atcctgtaca
     2641 agaaaaagta gcaaaatttg ctgaaagtta tgggatgaag ctagaaaatg tcttgtttaa
     2701 tatggacgga acaattgaat tatatttacc atcgggagaa gtcattaaaa agaatatggc
     2761 agattttaca ggagaagcac ctcaaggaaa tggtgaaaat aaaccatctg aaaatagaaa
     2821 agtatctact ggaacagttg agaaccaacc aacagaaaat aaaccagcag attctttacc
     2881 agaggcacca aacgaaaaac ctgtaaaacc agaaaactca acggataatg gaatgttgaa
     2941 tccagaaggg aatgtgggga gtgaccctat gttagatcca gcattagagg aagctccagc
     3001 agtagatcct gtacaagaaa aattagaaaa atttacagct agttacggat taggcttaga
     3061 tagtgttata ttcaatatgg atggaacgat tgaattaaga ttgccaagtg gagaagtgat
     3121 aaaaaagaat ttatctgatc tcatagcgta aggaataaca gtagaaaaag tctgaatcaa
     3181 aaatgaagtt ctctcaaaag ttagaaataa aactctgact ttgggagaat ttcattttat
     3241 tattaatata taaaatttct tgacatacaa cttaaaaaga gg
//
LOCUS       DAVKJD010000116         3246 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKJD010000116 DAVKJD010000000
VERSION     DAVKJD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.116"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            115..426
                     /locus_tag="VW157_001899"
     CDS             115..426
                     /locus_tag="VW157_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553150.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNVSDKETAEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            430..1128
                     /locus_tag="VW157_001900"
     CDS             430..1128
                     /locus_tag="VW157_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9553151.1"
                     /translation="MKKYFIGGLGSNAYHSKDFFQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYLASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVLDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALFDTIFLENFGHELYTQAPK
                     EIASLMNEWLAYFL"
     gene            complement(1257..2072)
                     /locus_tag="VW157_001901"
     CDS             complement(1257..2072)
                     /locus_tag="VW157_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9553152.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(2069..2974)
                     /locus_tag="VW157_001902"
     CDS             complement(2069..2974)
                     /locus_tag="VW157_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9553153.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
BASE COUNT         1083 a          555 c          680 g          928 t
ORIGIN      
        1 aggaagtcta atgctaaaat cttatcagtg agaagatttc ttaaagttta ttctttgtag
       61 tttcatctgt attagtacat aatttaactt agagaaaata ggaggactaa tcgaatggaa
      121 ttaaaagatt ttacagaaaa agaacaggaa atgattaaga aaaggcttac aatgtctaat
      181 gttagtgaca aagaaactgc tgagaagatt ctggcgctag taccacaaga cttgattaag
      241 cgaatcccgt tttttgtcag aaaacatgct acaacacgta cgattaaacg catttcaatt
      301 gaacaccctg aactctacgc tgtagctcaa acaagtggtg agattccaga aaaagaacgc
      361 gaagaattgc gtcagattat cacaactatc tttgaacaaa agatgaataa gcatagtatt
      421 aagtagagaa tgaaaaaata ttttattggc ggtttgggaa gcaatgccta tcatagcaag
      481 gatttttttc aagaactaga ttcgcaggtc tattttctaa atccatatga aaagcatctt
      541 cgagatgaaa cagaattgaa atcatggttt aaaaatgaga ttgtagagga agaatctatc
      601 tgtctgatag gccattctct tggaggagat ttagctcgtt atctcgcatc ggaatttgaa
      661 gaagtaaaaa aactgattct tttggatggt ggctatctag atttagataa gattttacct
      721 ttggatacag agttagagga aactaaaaat tatatcaaat ctcaaattgt tttggactta
      781 gctcttctta tttctaaaga aaaatctgaa gcaaagcatt ggtcagaaaa tatggagaaa
      841 gctgtaagac agtcttatca ctggaatgtt gagtataata gatatgagtt ggctataaat
      901 tatgaaaata tagaagcgat actccgccta cggagaaaaa tacaagcttt taagagagaa
      961 gtgggagata ccttgtttat tagtcctcgc tatcctaatg aagctacatg gagagaggaa
     1021 gccctatttg atactatttt tttagaaaac tttggccatg agctttatac tcaagcacct
     1081 aaagaaatcg ctagtctaat gaatgagtgg ctcgcttatt ttctatgagc tacttgtatt
     1141 gctggaaatg gtataagatg aaaatctaca agtcactaat tttcatataa caaaaaacga
     1201 acaggctctt tttctgttat tcagaagaga acttgttcgt ttttatcttg attttcttaa
     1261 aaagctctat atacataggg attttctggt ttatcgactt tggtaaagct gtcgatttcc
     1321 aaggttggaa ggttttgttt gagttcttta tggtagtgat tgaccagttt tcctttggct
     1381 gttatccaag tgttgttttc atactgccgg gtattgccct tggtcagcaa tccatagaca
     1441 ccagaatctg cgatacagtg gataatgccg aagcggaaca gaaattggct attggcatga
     1501 ctggggtcgt tatagacaaa gcctgtaaac tggattgtct tgccctcaaa ctcatctgga
     1561 tagtcgtaga tagcctccat gacttccata tagttttcat tagtgatctg aatactatct
     1621 tgggataagt atttatccgc cgccgttcgc atttcctttt cataggctga ttttgaaaaa
     1681 taagaactgg tatctggttt caaatattgg cttgtcgtcc cttcgcttgt ctgaatggct
     1741 agatccgttc cttctgatag ggggaaatga taacctttag cagaaacggt ctgagaatcc
     1801 aagctaacag ttgggaaagt taagccgatg acaagtggaa tagccagaag agaaatactc
     1861 gtcatcttgg ctaatcggct gttcagatga ctgtgggttt tgacttgctt catccagata
     1921 tacaattgaa caatagccaa gataaaggaa agcaccatgg aaatataggc cagataggaa
     1981 tagtgcatgt tgatgtactg gtttagtttg cccgacagat ggaggtaaat agtcagttca
     2041 aaatagccag ctaaaactaa aaatcgaatc ataggatgac tccaatcaag agagaatagg
     2101 ctaaaacgac cagagtcaca atcgtaatga agtgactaat aaatcgtgct ttcaagtaat
     2161 ttttcatcat gagaatattt ttgatatcca gcattgggcc aatgacgaga aaggccagaa
     2221 ctggagccaa accgaaactc gagagtagag aagcacctat aaaggcatcc gcctcactac
     2281 agagcgaaag aagaaaggcc aaaagcatca agagcaggat ggcaaaaaga ggagtcgcac
     2341 tgatagaggt cagaatccga gtcggaacgt agacctgtat tatagaagca aagaggcagc
     2401 caaataccaa ataacgcccc gtatcaaaaa attcatcaat ggcctgcaca aagacttgaa
     2461 aaactttttt tgcagaactc aagtaagaaa aatcatgctc atgacaagcc agacgatttt
     2521 ctttctgaat cggttcttgc cagaaaaatc ctagaaaaat tcctagtatt acagccacaa
     2581 gaatggaacc cagagctcgt aatagggcga catggaagga gttgccaaag gcagaatagg
     2641 tcgcaaaaag aacaatggga ttgataacag gtgctgtcac aagaaaagga acggccgtgt
     2701 aacttggaac ctttttttcc agaaaacgat tgatgatggg gacgattcca cattcacaag
     2761 aagggaaaag tataccgaca aaggtcccaa aaaagattct cccccaacga tttcgaggga
     2821 gaaaatgata aaccttgtca ggtgtgatat aaacttcaat cagccctgag acaatgcttc
     2881 ctatcagaac gaagggaagg gcttcaatga taatggagag aaaaatggct ccagtttgaa
     2941 gtacactaga tgggagttgt tgaaaagcag tcatctagtt tttcttttct gctttgactt
     3001 ttttctcttt gtcatcaggg aaagtaactt gtttcaaatc agggagttta gcaaactctt
     3061 caaacatctt gtccaagtcg tctcgttttg taaatttgac agccataggt gtacctcgat
     3121 tctaaattct acctctatta tactattatt ttgcggaaaa gggagttttt taatttgttt
     3181 tcgatagttg atacacttcg aaaatctctt caaaccacgt cagcttcgcc ttaccgtagt
     3241 acggtt
//
LOCUS       DAVKJD010000117         3020 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKJD010000117 DAVKJD010000000
VERSION     DAVKJD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.117"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            59..511
                     /locus_tag="VW157_001903"
     CDS             59..511
                     /locus_tag="VW157_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9553154.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRLLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLERGKDSFKHKMSIYETALSE"
     gene            1343..1702
                     /locus_tag="VW157_001904"
     CDS             1343..1702
                     /locus_tag="VW157_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553155.1"
                     /translation="MGQEIKLIRKQFRITRQEEKQIKEMMREQKVDSFSEFLRQNLLK
                     KNYQDRIFESWFSLWQSQKFEQISRDVYEVLVVARENHQVTQEHVSILLTCVQELIAE
                     VNQVQPLSREFREKYMG"
     gene            1712..2041
                     /locus_tag="VW157_001905"
     CDS             1712..2041
                     /locus_tag="VW157_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEU9553156.1"
                     /translation="MVYRYRTNLKKVFLTDPELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSHLISQDQLQELSKGVGELIKE
                     VDKEFQV"
     gene            2064..3020
                     /locus_tag="VW157_001906"
                     /pseudo
     CDS             2064..3020
                     /locus_tag="VW157_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261819.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
BASE COUNT         1078 a          457 c          600 g          885 t
ORIGIN      
        1 ttctcactta ttaggtgcat ataaacgaaa aattttttct aaagaaaata cacgattttt
       61 ggagtttaat attaactcga aagaatcttc gcaacaactt gatattctct ataaagaagt
      121 tggtgtatta agtttgggac aaaaagttgt agccatgctt gattttttat tagcatatag
      181 tgattattct aaagacttca gactattgat tattgatcag cctgaagaca atctagacaa
      241 tcgttatatt tacaggcatt tagttcagca gtttagagat gtgaaagctc aacgtcaaat
      301 tattttagca acacataatg ctacaattgt aacaaattct atgacagatc aagttgttat
      361 tatggagtca gatggagtta acggatggat tgaatcacag ggatatgtta gtgaaaaata
      421 tataaaaaat catatcatca atcaattaga gagaggaaaa gattccttca agcataaaat
      481 gtctatatat gagacggctt tatcagagta gagtcagaaa aagtaggtta gaaatttagc
      541 ctactttttt ctttgtccga caggcatagt gtacatctga ggtccaagtc ctctgtggat
      601 gtttgctgca gatgaaacca atagcgactc ctaagcctga atatcgtgag gtagggggga
      661 taggaaggaa ttagcgaaat caaggttcta caaacagaat cgtgacttga agccatatat
      721 agcggatgag gaactctaaa atccaaatag gtgtcgtaac ctatatacgt aaattacgag
      781 agtaaactag gaaagatgta cggcttattc cgtgagcgtt taggacgtag tacaacgaat
      841 catgggagtc agctgaacac atagtattga agaaatttct gtaatggaaa tggagcgaag
      901 aagtgaacaa ttaaatgaat acctctctaa ttaaatttgt caattctaat tcctggtatg
      961 aaaagacagt gacctaaaaa tgtaaacgat gggagctgat cataaatata ggacggtaca
     1021 tgcagtggtg ttagagatta gtccctactt gatttgtgat aacttcccca aatttcttct
     1081 gctatacttt tctcaacttt taaaaatcca actaagaatt ttacctggga gtttgggggc
     1141 gtagccacca agttatctta tggttagctg tcaaaactgg aaggttttgg ttagctggcg
     1201 atatgatttt ttgggtattg tgaccacaat gcctgagctc gcaaagaccg aacaaggaga
     1261 gactgaggtc tttaggaatc gtactgacaa tgtgaggcag ccttacattg tcagacaagt
     1321 ataatgttga aagaatggta agatgggaca agaaattaag ttaatccgta agcaatttag
     1381 aatcacgaga caagaagaaa aacagataaa agaaatgatg agggaacaaa aagtggatag
     1441 tttctcagaa tttcttcgtc aaaatttatt gaaaaagaat tatcaggata gaatttttga
     1501 aagttggttc tccctttggc agtctcaaaa gtttgaacag attagtcgag atgtgtatga
     1561 agttctggtt gtcgcaagag aaaatcatca agtgactcaa gagcacgtct caatcttatt
     1621 gacttgcgtt caagaattga ttgcagaagt gaatcaagta cagccactca gtcgtgagtt
     1681 tcgtgaaaaa tatatgggtt aggaggatgt aatggtttat cgctatcgta ccaatctcaa
     1741 aaaagtattt ctaactgatc cagaattaca ccaattgaat gaacggattg ctaagagcaa
     1801 ttgtcaaaat ttttcagtct atgccagaaa agttttgctt aatcccaata tgtcatttgt
     1861 cacgattaac actgatactt atgaccagtt agtttttgaa ttgagacgta ttggaaataa
     1921 catcaatcaa attgcgcgtg cgattaatca aagccatctg atttctcagg accaattaca
     1981 agaattgagt aaaggagtcg gagagttaat taaggaagtg gacaaagaat ttcaagtgta
     2041 ggtgaaaaga ctgaaggagt ttcatggtag ttactaaaca ctttgcgacg cacggaaaaa
     2101 aatatcgtag gcgtttgatt aaatatatcc tcaatccaga taaaacagat aatctaaaat
     2161 tagtatctga ttttggcatg agtaattact tagattttcc tagccataca gaaatggtag
     2221 aaatgtacaa tgttaacttt actaataacg acaagttgta cgagtccaga aatgaccgac
     2281 aagaaaaaca ccaacaaact attcatgccc atcatctcat ccaatcattt tctcctgaag
     2341 ataatctgac acctgaagaa attaaccgca ttggttatga gaccatgatg gaattgacag
     2401 gaggtcgttt taagtttatc gtagcaactc acacagacaa agatcatgtt cataatcaca
     2461 tcctaatcaa cgctattgac cgcaattcag ataaaaagtt gatatggaac tatgccttgg
     2521 aacgaaacct ccgtatgatt tcagaccgta tttctaaaat ggctggtgca aaaattattg
     2581 agaaacgtta ttcttatcga ggttataaaa aatataggga atctagtcat aagtttgaat
     2641 tgaaacaacg tctttatttt ttgatgcagc aatctaaata gtttgatgat tttttagaga
     2701 aagcaaagca gttgcatgtt cagattgatt ttagtcaaaa gcatagtcga ttcatgatga
     2761 cggatcgagc aatgacaaaa cctattcgag gacgccaact cagcaaacga gatctatatg
     2821 atgaagaatt ttttagaacg cattttgcta agcaagagat tgaaagtcgt ttagaatttt
     2881 tgttgaaccg tgtcaattct ttagaagagt taataacaaa agcaaaagaa ttgaatctaa
     2941 ccattgactt aaaacaaaaa aatgtaactt ttatcctgaa agaaaataat caaaagataa
     3001 gtttgggtca tcaaaaaata
//
LOCUS       DAVKJD010000118         3012 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKJD010000118 DAVKJD010000000
VERSION     DAVKJD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.118"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            166..1176
                     /locus_tag="VW157_001907"
     CDS             166..1176
                     /locus_tag="VW157_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001834714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin formation protein"
                     /protein_id="HEU9553157.1"
                     /translation="MVINNIYDEYPIWSIPVNFKGSKWCLEQTGNDMYRGRAGILLFM
                     YYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGLTGDIGYFIVLSIIEDYDTNTLA
                     CLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINTLVRYYRRGIEKERVLRLVLSLG
                     DTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKVTRLKKYKELILQLVPREKIKIH
                     SLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGSMAIINFYIDYMKEMDTSMELES
                     LLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIAYTFLRYIALNQNDKKIVIPSLL
                     EI"
     gene            1526..2230
                     /locus_tag="VW157_001908"
     CDS             1526..2230
                     /locus_tag="VW157_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEU9553158.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWYYVENIK"
     gene            2211..>3012
                     /locus_tag="VW157_001909"
     CDS             2211..>3012
                     /locus_tag="VW157_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEU9553159.1"
                     /translation="MLKILNNSVIYLCLIPLLFLLLIIFPNDSLIYYFRLILISLISI
                     TLHELGHFLVGRCLSYKLEMLATPFFFYFRKKIYFKFPVLLAFGYCQMSNRNITNEKN
                     SDRNLVFYFFGGGGANLIVAILALLGFVPYASEFFILNIILFLVTVCLPIDGTDGNAI
                     REIVLYSKDSKTYQRFFANSLYNNPYITIDDFAKLTSKEKSFFSKFEKCLINLYFEVK
                     GEGSAFTIANHEVFDSNIQENIIHEYYKLLHKSSDWIIPQNLSLEEVVF"
BASE COUNT         1078 a          362 c          516 g         1056 t
ORIGIN      
        1 aatttggaaa atttagaaaa gcaacttgat atcattgaaa tttattttcc aagctttgat
       61 cagcgaaaaa aaggatataa attaaactta tctaatagaa aaaaactccc aaatgaaagt
      121 ttagttacta ttctgcataa tgaagtagaa aatattgcag actctttggt tattaataat
      181 atttatgacg aatatccaat ttggagtata cctgttaatt ttaaaggttc taaatggtgt
      241 cttgaacaaa cgggtaatga tatgtatagg ggtagagctg gaatattact gtttatgtac
      301 tatgtaaaat tattgaaaga taaatcatca tatactcaac tgtacaataa aatgttatct
      361 gcaattccgc catctgtttc tttatctaag accacatttg gtttaactgg ggatatagga
      421 tatttcattg ttctatccat tatagaggat tacgatacaa atactctcgc atgcttaaat
      481 tatataaaga gtgttttaac tgagcttgaa aagactgatt ttgcaagtgt ttcaaacaag
      541 tctgactata ttaatggtct tctaccttta attaatacac tggtaagata ttataggaga
      601 ggaatagaga aggaacgtgt tctaaggcta gtattaagtt taggtgatac gctatataat
      661 gatgtaagcg agagagataa tagtaatttt ggatatagtt ttggccatgg actatcaggt
      721 gtaattttta cattaaaaaa tatttataaa gtgacaagac ttaaaaaata taaagaatta
      781 attttacaac tagttcctag ggagaaaatc aaaattcatt ctttgaagtg gtgtagtgga
      841 gaatttggga cattagttaa tcatccccaa agtgttccag agggaattgt agctagttta
      901 gataatgata cattttgcca tggaagtatg gccataataa atttttacat cgactacatg
      961 aaagaaatgg atactagtat ggaattggag tctttattgt gtagtgttgt aatgaataaa
     1021 tcaaaagaag gagagtatcg tatcgtccaa acaagcaatt tcccagattt ttctttgtat
     1081 acaggtgtga ctggtattgc ttatactttt ttaaggtata tcgcactgaa tcagaatgac
     1141 aaaaagatag ttatcccctc attacttgaa atctagctta atttatagtt ttgtttctaa
     1201 aatagaggtg tccactcatt aagcaatata aaaataccaa gattgactag tttaatatgt
     1261 aattctataa aatattttta atagtgtagc ttttcgttga gataataata taataaagaa
     1321 aggttctatt ctaattgcta aacggcttga ggtatatgct actgttatag aaatgatttt
     1381 aaactaaaat caaaattact ccaaaaatag tattgatttt ggtttaatac aaaacatatg
     1441 taataatgtg agtcaacaaa agtctgtttt tgtaccatac tatcttgaaa aatctactaa
     1501 attcaaattg agaggatgaa ttaaaatgaa taaaatattt atatatgcag gagtgcgaaa
     1561 tcataactct aagactttag agtatactaa gagactttca agtatcattt ctagtagaaa
     1621 taatgttgat atatctttca ggacaccttt caattcagag ctagaaatat ctaattctga
     1681 ttcagaagag ttgtttaaga aaggaattga taggcaaagt aatgctgatg atggaggtgt
     1741 tataaaaaaa gagttattag agtctgatat tataattatc tcttctcctg tatatttaca
     1801 aaacgtatct gtggacacaa aaaactttat tgaaaggatt ggggggtgga gccatctttt
     1861 tcgattagct ggaaagtttg ttgtaacatt agatgttgct gagagtaacg gctctgataa
     1921 tgtgtcagaa tatcttagag atatattttc atatatggga ggacagatat tacaccaagt
     1981 ttcaattacc aattcactca aagatattgc agaggctcag ttgatggagg caacttataa
     2041 aattgaagat gtacttgaag ggaaaattaa gtacaaaaca acggattatc aagaaagagc
     2101 ttaccagaca ttaaaattaa ttttagaaaa ttatgattcc gaacattttg agaagatgta
     2161 ttgggagaaa aaacgccttt ttgaagctaa tagtttagag gaatggtatt atgttgaaaa
     2221 tattaaataa ctctgtcata tatttatgtt tgattccgtt attgttccta ttattgataa
     2281 tttttccaaa tgattcatta atctattatt ttcgattgat tttaatttcg ttaatatcaa
     2341 ttacattaca tgaactaggt cattttttag tgggaagatg tttatcttat aaactagaga
     2401 tgcttgctac tccctttttc ttctatttca gaaaaaaaat atatttcaaa tttccagtgt
     2461 tattagcatt cggttattgt caaatgagta atagaaacat tactaatgaa aaaaatagtg
     2521 ataggaattt ggtgttctac ttctttgggg gaggtggagc taacctaatt gtcgcaatat
     2581 tagcactttt gggatttgtg ccatacgcat cagaattttt tattttgaat ataattcttt
     2641 ttctagttac ggtatgttta ccaattgatg gaacagatgg caatgctatt agagagatag
     2701 ttttgtattc aaaagattca aaaacatatc agagattctt tgcgaacagt ctctataata
     2761 atccttatat aacaatcgac gattttgcaa aattaacctc taaagaaaaa tctttttttt
     2821 caaaatttga aaaatgtttg ataaaccttt actttgaagt gaaaggtgaa gggtcagcat
     2881 ttacaattgc taatcatgaa gtttttgatt caaatattca ggaaaatatt atccatgagt
     2941 actataaatt actacataaa tcttctgatt ggattattcc acagaactta tctttagaag
     3001 aagttgtttt ta
//
LOCUS       DAVKJD010000119         2960 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKJD010000119 DAVKJD010000000
VERSION     DAVKJD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.119"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="VW157_001910"
     CDS             complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="VW157_001910"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9553160.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRSKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1420..2196
                     /gene="recX"
                     /locus_tag="VW157_001911"
     CDS             1420..2196
                     /gene="recX"
                     /locus_tag="VW157_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9553161.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2285..2818
                     /locus_tag="VW157_001912"
     CDS             2285..2818
                     /locus_tag="VW157_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9553162.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT          953 a          538 c          531 g          938 t
ORIGIN      
        1 cttttcaaac tttgtcaact acttttttaa acttttttta ttaattttac aacagcttca
       61 gttcgagctg tatgtgggaa catatcgacc gactggatat aatgaagatc atagacttct
      121 actaagcgta ccaaatcacg agccaaggtc gaaacattac aagaaatata aaccattttt
      181 tctggtacat aagtaagaat agtatctaat aacttatcat ccagacctgt acgtggtggg
      241 tcaacaatca aagcatctgc tcggtagcct tccttgtacc aacgaggaat aatctcttct
      301 gccgttccag cttcataatg agtattgtca aatcccattc ttttagcatt tcgcttggca
      361 tcttcaatag cttctggaat aatatccata cctctgagtg tttttacttt ctttgcaaag
      421 gcaaatccaa tcgttccaac tccacaataa gcgtcaatca aatggtcttc tttatcaaca
      481 tccagcgctt ttactgcttc gctatagagg acttctgttt gctcaggatt tagttgataa
      541 aaagctcgag gggatagtga aaattcataa ttgagtacac cttcttgaat actctcttgc
      601 ccccagataa tctctgtctt ttcaccatat atctcactgg ttttagctgt atttgtatta
      661 acagctactg tcacaacttc tgggaaatct ttaaccaact cttttaccaa ttgagttaaa
      721 ttaagctggc ggtttgtaac aataataatc tgaacctgtc cggtctttct cgcgcgtcgg
      781 accataatag tacggacacc tagaactttt ctctcatccg tgattggaat ctggtgataa
      841 gtaagtaatt ctgctaagcg attagcaatc acttgggttt ccttatcttg taccaggcag
      901 tctttcaact ctactaaata gtgagagttt tgtgcatata agcccgcctt gacctgattt
      961 ttaaattttc gagtctgaaa ttgtaactta gatctgtaat attttggttc ctgcattcca
     1021 atagttggac gaatttcata attttcatat cctgcaggag caaatttttt cagcgcttga
     1081 tgaagtaagt ccgtcttgaa ctccagctgc ttatcataat gcaggtgcat gatttggcag
     1141 cctccgcatt cattataaat agtacaagat ggcacaattc gaaatttaga cttcttgttg
     1201 accttcagta attttgcttc aacaaagttg cgtctaatag aagtaatctg acaatagata
     1261 tcttcgcctt tgagagctcc tggtacaaag actaatgttt tttggtaaaa accgattccc
     1321 tcaccgttaa ttcccatgcg cttgattttt aatggtattt tttgtttcac tttcagattc
     1381 atacccctat cttatcacat tttgagttat aatagagcta tgaaaatcac aaaacttgaa
     1441 aagaaaaaaa gactctatct gatggagctt gataatggcg acaaatgcta tatcaccgaa
     1501 gatacaattg ttcgttttat gttatcgaga gataaggtga taagcgaaga ggaattgaaa
     1561 gagattcaag actttgctca attttcttat ggtaagaatc tggccctcta ccacttatcc
     1621 tttaaagcac gcactgaaaa agaagtcaga gaatatctga aaaaatacga tattgataaa
     1681 aacatcgttt ctcaagttat tgctaatctt aaagaagata agtggattaa tgatggtcag
     1741 tacgcttatg ctatcatcaa taccaatcaa ctttcaggag acaagggtcc ttatgtgctg
     1801 actcagaaac tagctcaaaa agggatttca aaatctacta tagaagagaa cttgaaagaa
     1861 tttgattttt ctgaagttgc tcaacgtgta gctaataaac tattgaaaaa atatgaggga
     1921 aaacttccag ctcgtgcctt gcaagataag attatccaga acttgactaa caagggcttc
     1981 tcttattctg atgctaaaat tgcctttgac gacttagata gccaagttga ccaagaaacg
     2041 actcaagaac tcatcttcaa ggaacttgat aagcaatata ctaagtatgc tcgaaagtat
     2101 gaaggatacg aactgaagca gcgtttaact caagttttag cacgaaaggg ctacgatttt
     2161 tcggatatag caagcgctct tagagaatat ctttaatatt tccaagtgaa attcacagat
     2221 tttaggtaat tttatggtac aatagtaaat gataaactta taaattgtag aaagttggtt
     2281 agttatgaag cttccaaaag aaggcgactt tattacaatt caaagttata agcatgatgg
     2341 gagtctccac cgaacttggc gggacaccat ggtactaaaa acaacagaaa acgccattat
     2401 tggtgtcaac gatcatacac ttgttaccga aagtgatggt cgtcgttggg tcactcgaga
     2461 accggctatt gtttactttc acaagaaata ttggtttaat atcattgcca tgattcgtga
     2521 taatggaact tcttactatt gcaatatggc tagcccctac tatctggatg aagaagcact
     2581 gaagtatatt gattacgatt tggatgttaa aatttttaca gatggggaaa aacgtctctt
     2641 ggacgttgaa gagtacgagc gtcacaaacg caaaatgaat tattctgatg acttggacta
     2701 tattttaaaa gaacatgtca aaattcttgt tgattggatt aacaatggac gtggtccttt
     2761 ctcagaagcc tatgtaaaca tttggtacaa gcgctatgta gaactaaaga atcggtaaag
     2821 ttgtcaaagg tcaggaacaa aatcctgacc tttttttggt agtataaaat aaattgtgta
     2881 aacacaaaaa ggaataaatc tattatagca gagttacaac acattactag aaagagattt
     2941 atgcaaaatt cgtaaaatat
//
LOCUS       DAVKJD010000120         2955 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKJD010000120 DAVKJD010000000
VERSION     DAVKJD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.120"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            125..931
                     /locus_tag="VW157_001913"
     CDS             125..931
                     /locus_tag="VW157_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9553163.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            897..1367
                     /locus_tag="VW157_001914"
     CDS             897..1367
                     /locus_tag="VW157_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU9553164.1"
                     /translation="MRMPLKHPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVAHQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQIGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(1554..2417)
                     /locus_tag="VW157_001915"
     CDS             complement(1554..2417)
                     /locus_tag="VW157_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9553165.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVETHYQPKKL"
BASE COUNT          952 a          447 c          635 g          921 t
ORIGIN      
        1 tatgtaattt tcctaggttt ttctgtttta aattgaaaaa aattacaatt taggcttgac
       61 aaaggatgaa gataggtata ttatttatac aaacaaaagg aataaacata aaggaggctt
      121 tgttatgaat aagatgaaga aggtgttgat gacgatgttt ggtttagtga tgctccccct
      181 actatttgct tgtagtaaca atcaatcggc tggaattgaa gccatcaagt ccaaaggaaa
      241 attggttgta gccctcaatc cagattttgc tccatttgaa taccaaaaag tggttgatgg
      301 gaaaaatcag attgtgggtt cagatatcga attagccaag gctatcgcaa cagaactagg
      361 tgtcgaattg gaactatctc ccatgagttt tgataatgta ctggctagtg ttcaatcagg
      421 aaaagccgac cttgccatat caggtgtttc taagacagat gaacggagca aggtgtttga
      481 cttttccatt ccctactata ctgcaaaaaa taaactcatt gtcaaaaaat ctgacttgac
      541 tacttatcag tctgtaaacg acttggcgca gaaaaaggtt ggagcgcaga aaggttcgat
      601 tcaagagacg atggcgaaag atttgctaca aaattcttcc ctcgtatctc tgcctaaaaa
      661 tgggaattta atcacagatt taaaatcagg acaagtggat gccgttatct ttgaagaacc
      721 tgtttccaag ggatttgtgg aaaataatcc tgatttagca atcgcagacc tcaattttga
      781 aaaagagcaa gatgattcct acgcggtagc catgaaaaaa gatagcaagg aattgaaaga
      841 ggcagtcgat aaaaccattc aaaagttgaa ggagtctggg gaattagaca aactcattga
      901 ggatgccttt aaagcatcca ttgaaaaata gaaagaagga aaagaacatg agtaaagaaa
      961 aagtaatttt ggcctattct ggcggtttgg acacatcagt tgctattacc tggttaaaga
     1021 aagactatga tgttgtttca gtttgtatgg atgtgggtga agggaaagac ttagatttca
     1081 tccatgataa ggctcttaag gttggggcag tagaatctta tgtcattgat gttaaggacg
     1141 aatttgctac agattatgtg ctagtggctc atcagtcaca tgcctattat gaacaaaagt
     1201 atcccttggt atctgccttg agccgccctc tgatttctaa aaaattggtt gaaatagcgc
     1261 atcagatagg agcgactaca attgctcatg gttgtaccgg taaggggaat gaccaagtcg
     1321 aataccagat tgcagtcgct aagaaagcga atgaagctaa aaagtgatgg agtgatgggt
     1381 tcagaataag aggttggtca attggcagcc tctttcttgt cgttgaaaaa gtgagatata
     1441 ttgacttttg aaaaaaatgt cataattgag ttgggataaa cgtaaaagtc atactttctc
     1501 gttaaatcgt tcggcgattc tatgaaaagg tcgaggagct ccctcgactt tttttataat
     1561 ttctttggtt gataatgggt ctctacttct tttttgagat gagacagcat agaagtttcc
     1621 tcggttagct ccagaaggga gaaccctttt tggataagtt tagaatcgtc tgtacaaatc
     1681 ccaatacgga catagcagat agccagccta tcgtagcttt tcttattctt ggcgtcctct
     1741 agtaaagtgt agcagatttg cttacacatg tttttatctt gattgtatag atagagtgtg
     1801 gaaaggttta atagaattgt cattcgcaag tcatataaat gttgatagtt tttatagact
     1861 tctaggcgtt gcaatatttt tccagtgatg agatggaggt attcaatggg aaaactgaaa
     1921 agaatggtat tgaggatttt tagatcactc tcataccatg tatcttgttt ttcaattttt
     1981 ttccaaagtt ttttgacaac ctgttctgcg tgttttgata gttcccctgc cccgtgttga
     2041 cggagataaa taacaacttc aagcatatct ctgatttctt ctatagggag atcgtggtgg
     2101 gtcttgaggt agtcttggca tttttgaaat aaattgacaa gatcgctggt cccgataatg
     2161 gaacgcatat tgagataggt ttgcataatc tctgtgcgtt gacttggttg ataaagttga
     2221 cagatatatt caaactcttc aaaactcata ttgatttgac ggagaagaaa ttccatattt
     2281 tcatattttg gggttgtctt gccactttcg atttttgata agctggttct tgagagaaca
     2341 cccccacaga cctcttcttg ggtcagcccc tttgactcac gtatttcttt atagaccttt
     2401 ccgaaatcat aacgcatgat tttctccttt tcgtgaaaaa agttaacaaa caataaaatt
     2461 ttgttaaaaa tattgtatca tagcaatgta aaaagtaaaa aagaaagtcg aaataaattt
     2521 gtgaaaaaag ttaacgtaat tttaaaatga gcttaataag aggtactatg taggtagaaa
     2581 ataaagatag gagaaaaagt atgaataaaa aaatattttg tattctagta tgtattatat
     2641 tattgattag tctggctata atctttccat ggggctggcc gatataatgt aagggagttt
     2701 gagttgttaa aggaggctca gtccttgggc aaaaatctgc tgtactacaa tgcttggaaa
     2761 gatatcaaga acaaagaatg atacggttga aaataaatta attcaagaac tactgggcag
     2821 tgattgaggt ttgggtgaca tcaaagaaaa gttagattaa ttctaaagaa ttgagtattt
     2881 ctatgttaaa aaaatatcct tgtacgatgc aacatgacca gtcagattgt gctgcggcag
     2941 ttgtttcaac agttc
//
LOCUS       DAVKJD010000121         2868 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKJD010000121 DAVKJD010000000
VERSION     DAVKJD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.121"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            38..718
                     /locus_tag="VW157_001916"
     CDS             38..718
                     /locus_tag="VW157_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9553166.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLETISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
     gene            678..1058
                     /locus_tag="VW157_001917"
     CDS             678..1058
                     /locus_tag="VW157_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU9553167.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESG"
     gene            1068..1355
                     /locus_tag="VW157_001918"
     CDS             1068..1355
                     /locus_tag="VW157_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9553168.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            1356..2114
                     /locus_tag="VW157_001919"
     CDS             1356..2114
                     /locus_tag="VW157_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9553169.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDILLDGQSIVENKKDFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKAQGRDKKEVTEEALQLLERVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAITDRIIFLDQGKI
                     AEEGTAQAFFTNLQTKRAQEFLNVFDFSQFGSYL"
     gene            2126..2868
                     /locus_tag="VW157_001920"
                     /pseudo
     CDS             2126..2868
                     /locus_tag="VW157_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
BASE COUNT          798 a          653 c          578 g          839 t
ORIGIN      
        1 caataactgt attatttcta gatgggaggt tctatttttg gattggtcca ttgttgaaca
       61 atatctacca ctatatcaaa aggcattctt tctgaccttg catattgcag tttggggaat
      121 tttgggatcc tttctgctcg gtttaatcgt tagtatcatc cgacattatc gaatccctgt
      181 tttggcgcaa gtagcgacag cctacattga attgtcacgt aatacgcccc ttttgattca
      241 actcttcttt ctctacttcg gtcttccccg aatcggcatt gtcctatctt cagaagtctg
      301 tgcaacgctt gggcttgtct ttttaggagg ctcctatatg gcagaatctt tccgaagtgg
      361 gctggaaacc atcagtcaaa cccagcagga gattggcctc gctattggtc tgacacctct
      421 acaggtcttt cgctatgtgg ttcttccgca agcaacagcg gtggcactcc cctcctttag
      481 tgccaatgtc attttcctta tcaaagaaac ctctgttttc tcagcagtgg ctttggccga
      541 cctcatgtac gtcgccaagg atttgattgg tctctactat gagacagaca ttgcgctagc
      601 tatgttggta gttgcttatc tgatcatgct gctacccatc tcactggtct ttagctggat
      661 agaaagaagg acccgccatg caggattcgg gaatccaagt actctttcaa ggaaataatc
      721 tcctgagaat cttacagggg ttgggcgtta cgattgggat atccatcctg tctgtcctct
      781 tatccatgat gttcggaaca gtcatgggaa tcatcatgac ctcccattct agaatcatac
      841 gatttttaac acgattgtat ctggacttta tccgtatcat gccccagctg gtgctactct
      901 tcatcgttta ctttggcttg gctcgaaact ttaatatcaa tatctcaggt gagacttcag
      961 ctattatcat ttttaccctc tggggaacag ctgaaatggg agacttggta cgtggagcta
     1021 tcacttctct ccctaaacat cagtttgaaa gtggataggc actcggcttg actaatgttc
     1081 aactttacta ccacatcatc atcccacaag tcttaagaag actgctaccg caggctatca
     1141 atcttgtcac tcggatgatt aaaaccactt cattagttgt tttgattggg gttgtggaag
     1201 tgaccaaagt tggacaacaa atcatcgata gcaatcgcct gaccatccca actgcttcat
     1261 tttggattta tggaaccatt ctagtcttat atttcgcagt ttgctaccct atttccaaac
     1321 tatccactca cttagaaaaa cattggagaa actaaatgtc tgaaactatc ttagaaatca
     1381 aggaactaaa aaaatccttc ggagacaatc ccatcctcca aggactttct ctagaaatca
     1441 aaaaagggga agttgttgtc atcctggggc catctggttg tgggaaaagt accctccttc
     1501 gttgcctcaa cggcttagaa agtattcaag gtggagatat tcttctggat ggtcagtcta
     1561 tcgttgaaaa taaaaaagat tttcacctag ttcgccaaaa gattggcatg gtctttcaaa
     1621 gttatgaact ctttccccat ctggatgtct tacaaaacct catcctaggc cctatcaaag
     1681 ctcaaggaag ggacaagaaa gaagtaacgg aagaagcttt gcaattacta gagcgtgtcg
     1741 gtttgctgga taaacaacat agctttgccc gtcaattatc tggtggacag aagcaacgtg
     1801 ttgcaattgt ccgtgccctc ctaatgcatc cagaaatcat cctttttgac gaggtgactg
     1861 cttcgctgga tccagaaatg gtgcgtgagg tgctggaact tatcaatgat ttggcccaag
     1921 aaggccgtac catgatttta gtaacccacg aaatgcagtt tgcccaagcc attactgacc
     1981 ggattatctt cctcgaccaa gggaaaatcg ctgaagaagg aacagctcaa gccttcttta
     2041 ccaatctgca aaccaaacga gcccaggaat ttttaaacgt ctttgacttt agccaattcg
     2101 gctcatatct atagaggaga ttcttatgaa actattcaaa ccactcttaa ctgttttagc
     2161 acttgccttt gcccttatct ttatcactgc ttgtagctca ggtggaaacg ctggttcatc
     2221 ctctggaaaa acaactgcca aagctcgcac tatcgatgaa atcaaaaaaa gcggtgaact
     2281 gcgaatcgcc gtgtttggag ataaaaaacc gtttggctac gttgacaatg atggttctta
     2341 ccaaggctac gctacgatat tgaactaggg aaccaactag ctcaagacct tggtgtcaag
     2401 gttaaataca tttcagtcga tgccgccaac cgtgcggaat acttgatttc aaacaaggta
     2461 gatattactc ttgctaactt tacagtaact gacgaacgta agaaacaagt tgattttgcc
     2521 cttccatata tgaaagtttc tctgggtgtc gtatcaccta agactggtct cattacagac
     2581 gtcaaacaac ttgaaggtaa aaccttaatt gtcacaaaag gaacgactgc cgagacttat
     2641 tttgaaaaga atcatccaga aatcaaactc caaaaatacg accaatacag tgactcttac
     2701 caagctcttc ttgacggacg tggagatgcc ttttcaactg acaatacgga agttctagct
     2761 tgggcgcttg aaaataaagg atttgaagta ggaattactt ccctcggtga tcccgatacc
     2821 attgcggcag cagttcaaaa agacaaccaa gaattgctag acttcatc
//
LOCUS       DAVKJD010000122         2825 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKJD010000122 DAVKJD010000000
VERSION     DAVKJD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.122"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..502)
                     /locus_tag="VW157_001921"
     CDS             complement(<1..502)
                     /locus_tag="VW157_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553170.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLN
                     MNRNGCMGDFPHDFFDIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSN
                     FDDFLEK"
     gene            complement(516..1229)
                     /locus_tag="VW157_001922"
     CDS             complement(516..1229)
                     /locus_tag="VW157_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9553171.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1719..1826)
                     /locus_tag="VW157_001923"
                     /pseudo
     CDS             complement(1719..1826)
                     /locus_tag="VW157_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2248..>2825)
                     /locus_tag="VW157_001924"
     CDS             complement(2248..>2825)
                     /locus_tag="VW157_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9553172.1"
                     /translation="TVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLAT
                     VKYYVEHPNERPHSDNGFGNASDHVQRNKNGQADTNQTEKPQTEKPEEETPREEKPQS
                     EKPESPKPTEEPEEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLT
                     GLKNNLLFGTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          874 a          534 c          450 g          967 t
ORIGIN      
        1 ttttttctaa aaagtcgtca aaattagaaa aaaatcgaaa atacaatgca ttctcttggt
       61 gtaaaatcgc tctttttaaa ggataatact ctttaatatt attaactttt tgttcgtagg
      121 catattttgc tacatgatct aggtatatat caaaaaagtc atgaggaaaa tctcccatac
      181 agccattacg attcatattg aggctatatt ttcgattagt aagataattc ggaaatatat
      241 ttcctattac tctaagatta tctacttgtg aatcccttct cccaatataa tccccttctt
      301 tgactaatga tggagatttc caagtaaaaa ttaaatcgcc agagaaattt ttcttatcaa
      361 aaaacttaaa aagagattga taaatactat ttgctaattg acaagtgtca gatgtaaatt
      421 cataattaac agcttcatta aaatcaactt cagacatttc aatgtactct tcaatagtaa
      481 tattagcttt ttttgtagtc attagctaat ctcctttaaa atgtgatctt ttcaagaata
      541 tttttatcta aacaatccag caagtcttgg taagaataga cttcgtaagt cggctgggct
      601 tgtgtgtgat tttcgaggtg atgaggatta taccagatag tgtcaatccc cgcattattg
      661 ccaccttgaa tgtcggcggt tagagaatct ccaatcatca gcgtcttttc tttactaaat
      721 ccagcaattt gctggccaat cttttcataa aagagagcat ccggcttttg agtttgcaac
      781 tgttctgaga taaagacttg attgaaataa ggtactagac cagattgagc caaacgtcct
      841 gtctgaatgg cagtaatgcc atttgtcgca gcatacaagt tataatcacg ctcaatgagg
      901 ctgtccaaga gatcatgagc gcccgatagt gtttgtccct gctgagcgag ataaaattgg
      961 taacgctggg caagaaaact accgtctttt tcctgtccaa aatgagaaaa taaacgagaa
     1021 aagcgcgtgt taaccagctc ttgtttactg attttcttca gctccaagtc tttccagaga
     1081 gccttgttca taggaacgta ataatcttta taagcctgaa tatccgcaac tccttcttct
     1141 tttagaagtt gggtcaaagc cacatcctca gcagcatcaa aatcaagaag agtgtggtcg
     1201 aggtcgaaga gtagaaattt gtagaacaat ttgaggtttt cctttctgaa aattcattaa
     1261 gaacattata tcataaagca cctcatacaa ttaactaatt taatcactta aaaaaaattc
     1321 gaacactttc tatacaactg acagctcaaa tctttcagaa tagaacaata ctaactatcg
     1381 agcaccccgt cttcataaat atatgtgtaa ttctagacct agaattccta taaactaaat
     1441 gctttcatac tcttccaagt aattgattgc cttaaatttt aatttttgaa ggattctaaa
     1501 gctagaatag ccccatcaca atcagttttg attgattcac aatttagaaa cactatagtt
     1561 tcactcctgt taaaataaaa aggaactgca taaagcaatc cctttctgat tttgaaatca
     1621 tttacttaac attttatagt tgagataatc aatagcttac ctataaaaag agttatagta
     1681 aaattcctta tttattgatt tcaagctccg ctaactgtat aagtaatcgg aacatcttta
     1741 gctactttac taatcagttc aacaacacct tctgcaatgt gtttggaatg tgaaacgata
     1801 acaagaccaa taccaatact ttccatcaaa ccactccagt ttctaaaata gcagtaaaga
     1861 gtaatcctga tgagaatgat ccaggatcaa tatgtccaag aaaccacatg ctcctaagac
     1921 aagagctaac agactggcca tcaataatag tattgttctt tttttcatca ttactcctta
     1981 actagtgttt aactgattaa ttagccagta aatagtttat ctttatttac gctatctgtt
     2041 aagatatagt aaaatgaaat aagaacagga cagtcaaatc gatttctaac aatgttttag
     2101 aagtagaggt atactattct agtttcaatc tactatattt tgcacatttt catgaaaaaa
     2161 atgagaacta gaactcacat tctgctctca tttttcgttt tcccgttctc ctatcctgtt
     2221 tttaggagtt agaaaatgct gctaccttta cttactctcc tttaataaag ccaagagttt
     2281 ttcagcttct gccataatag tattgttgtc ctgggtgcca aatagtaaat tattttttaa
     2341 tcctgtgaga gtctctttgg cattggactt gataattgga tcctggattt ttccaagtaa
     2401 atcttcagcc tctctcagtt tttcttcaac cttttcagtc tcgacctgag gttcttctga
     2461 ttcctctggt gattcttctt ctggttcctc tgttggtttt ggagactctg gtttctcgct
     2521 ttgcggtttc tcttctcgag gggtttcttc ctcaggtttt tctgtctgag gtttctccgt
     2581 ttgattggta tcagcttgac catttttgtt tctttgaaca tggtcgctag cgttaccaaa
     2641 accattatct gaatgcggac gttcgtttgg atgttcgaca tagtacttga cagtcgccaa
     2701 aagatcctca agagtatacc ccttaggtgc ctcataaagg ccttcgtcaa accactcaaa
     2761 tttgatgtta tggtaatggt cataatgagg tatgattaaa ctaccgtttt tgacttctac
     2821 agtat
//
LOCUS       DAVKJD010000123         2750 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKJD010000123 DAVKJD010000000
VERSION     DAVKJD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.123"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            332..679
                     /gene="ssrA"
                     /locus_tag="VW157_001925"
     tmRNA           332..679
                     /gene="ssrA"
                     /locus_tag="VW157_001925"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(923..1699)
                     /locus_tag="VW157_001926"
     CDS             complement(923..1699)
                     /locus_tag="VW157_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HEU9553173.1"
                     /translation="MNATDIKNTYLNYIKENAVFNDVTDTHTEVITPFIDPLGEAIGF
                     SIKSNGKHLTVTDDGYTIWNLSINNIDVTKKGRRQDIFNSLLHFNGFDLHDGAIERTT
                     GKEHLGQVIHDMTQLLMNVYDFIQLTPNNIKSQFLDDVKSYFMKNEHYTVFPAFSIAG
                     KSRLEHRFNFVFMSKGISKIARVHNNITKQQVDTILASWLDTSEYRRKEYGDTEQLYI
                     IVSDEGYNNIKDDHQIALQEYGINILNFSDKEQLEIQLGK"
     gene            complement(1726..2196)
                     /locus_tag="VW157_001927"
     CDS             complement(1726..2196)
                     /locus_tag="VW157_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553174.1"
                     /translation="MKELLLTDSEAKALIKIIKAIANKHNRTLTNRSSGTINLVGKHD
                     TRFILNYFYSTTSKVFHLRETQHNYTLLRINLNNKFHKNANGEKVWGNRVNIFSEEEY
                     YQKGDETTHYKAYPLPYEDILNTDDFLDMLDNLLDYTNVNNPENIAINIQEDLL"
     gene            complement(2251..2715)
                     /locus_tag="VW157_001928"
     CDS             complement(2251..2715)
                     /locus_tag="VW157_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEU9553175.1"
                     /translation="MSKVVKVTGAEVVISHNEEYLKVNPSELNFVPKLGDEVEVHKVD
                     GEIIVIKVKDKKDDKININIVNENNAMQNQSQVVHTQEIATGVHYVNKWVYVILALFL
                     DGLGIHHFYAGYNGKGFLFLILSLTGIPAIIALFQGIIALFKKPDVYGRIAV"
BASE COUNT          816 a          446 c          472 g         1016 t
ORIGIN      
        1 gtgcaataag tgaaaagtag tccgaataat ataaggattg attttatagt ttttaaactc
       61 aaatgaatta aaataaagag agtacgaaaa ttctcatctg aaagtatttt agaataattc
      121 tcttcgtgaa tttcttcaaa acagatagct tcatcttagg tatgtgttaa ttggatttcg
      181 taagctctat ttacaatcgt aaagttatgc actgagaaag ttgtagttag tcttccagtt
      241 tgctaaattt ctcgaatagt atctttttta ggtatgtgat ttctttttgc atttttgagt
      301 tagataaggt ataatggttt tattgtcttt tggggtcgtt acggattcga caggcattat
      361 gaggcatatt ttgcgactcg tgtggcgacg taaacgctca gttaaatata actgcaaaaa
      421 ataacacttc ttacgctcta gctgcctaaa aaccagcagg cgtgacccga tttggattgc
      481 tcgtgttcaa tgacaggtct tattattagc gagatacgat taagccttgt ctagcggctt
      541 gataagagat tgatagactc gcagtttcta gacttgagtt atgtgtcgag gggctgttaa
      601 aataatacat aacctatggt tgtagacaaa tatgttggca ggtgtttgga cgtgggttcg
      661 actcccaccg gctccattat tcctttgcat tcttttgcat tccttggtaa aacgttgtta
      721 aatcaacgtt ttttattttt atctttggta ttcctttgca ttcttttgct aaaaagggag
      781 tcacaaacag accctatttt aaaaaaagga tagaaaaaag gatacaacat ttgtcgcatc
      841 ctagaaatct atgtacttcg taaagcgttc tccgattcga gtattcctct gcatgcttta
      901 gtaaatatag gattttttgt agttacttcc ctaattggat ttctaattgt tctttatctg
      961 agaaattcaa aatgttaata ccatactctt gtagagcgat ttgatggtca tctttgatat
     1021 tattataccc ttcatcgctt acaatgatat acaactgttc tgtatctcca tattcttttc
     1081 tacggtattc agatgtatct aaccaactag ctaagatagt atcaacttgt tgtttggtta
     1141 tgttattgtg aactctggct atttttgaaa tacctttact cataaacaca aaattaaagc
     1201 ggtgttctaa acgagattta cctgcaattg agaaagctgg aaagactgtg taatgctcat
     1261 ttttcatgaa gtaagattta acatcgtcta aaaattgaga tttaatattg ttaggtgtta
     1321 gctgaataaa gtcataaaca ttcatcagca attgtgtcat atcatgtatg acttgtccta
     1381 gatgttcttt accagtcgtc cgttcgattg ctccatcgtg taaatcaaat ccattaaagt
     1441 ggagaagtga gttaaagatg tcttgacgtc gccctttttt tgtaacatca atattattaa
     1501 tggataaatt ccagatagta taaccatcat ctgtaactgt caaatgtttt ccattgcttt
     1561 ttattgagaa accaatagct tcacctaatg ggtctataaa aggagtaatc acttcagtat
     1621 gagtatcagt cacatcgtta aaaacagcgt tttcttttat gtaatttaga taggtatttt
     1681 tgatatcagt agcattcatg atattctcct ttctttatag ggtgattata acaaatcctc
     1741 ttgtatatta attgctatat tttcaggatt gttcacattg gtatagtcaa gcaaattatc
     1801 aagcatatcc aggaagtcat cagtgttaag aatatcttca taaggtagtg gataggcttt
     1861 gtaatgagtt gtttcatccc ctttttgata atactcttct tcagagaaga tgttaactcg
     1921 attgccccaa actttctcgc cgtttgcgtt cttatggaat ttattattta gattgattcg
     1981 taagagagta tagttgtgtt gagtttccct tagatgaaaa actttgcttg tagtgctgta
     2041 gaagtagttc aaaataaatc tagtatcatg cttgccaaca aggttaatag tacctgaact
     2101 gcgattcgta agagttcgat tgtgtttatt agcaattgct ttaattattt ttatgagagc
     2161 tttagcttca ctatctgtca gaagtaactc tttcatatct ttcctttcta ctttatctac
     2221 attctagaga gtggaaatct ggaatgtaga tcagacagcg atccttccgt atacatctgg
     2281 tttcttaaag agagctatga tgccttggaa aagtgcaata atagcaggta ttcctgttaa
     2341 agataaaatc agaaaaagga aaccttttcc gttatatcct gcgtaaaaat gatgtatacc
     2401 caatccatct aaaaataaag caagaataac ataaacccac ttattaacat aatgtacacc
     2461 tgtagctatt tcttgagtgt gaacgacttg tgactgattt tgcatagcgt tgttttcatt
     2521 gacaatgtta atattaattt tgtcgtcttt tttatcttta acttttatga cgataatctc
     2581 accatctact ttatgaactt cgacttcgtc acctaatttt ggaacaaaat tcaattcaga
     2641 tggatttact tttaaatact cttcattatg ggagattaca acctctgctc ctgtaacttt
     2701 aacaactttt gacattttgt ttttcctttc attttaccat ctatagattt
//
LOCUS       DAVKJD010000124         2508 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKJD010000124 DAVKJD010000000
VERSION     DAVKJD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.124"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(114..437)
                     /gene="tnpB"
                     /locus_tag="VW157_001929"
     CDS             complement(114..437)
                     /gene="tnpB"
                     /locus_tag="VW157_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9553176.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
     gene            complement(698..997)
                     /locus_tag="VW157_001930"
     CDS             complement(698..997)
                     /locus_tag="VW157_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9553177.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1162..1569)
                     /locus_tag="VW157_001931"
     CDS             complement(1162..1569)
                     /locus_tag="VW157_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9553178.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATPFKEVQKPLENFN
                     HIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            1695..2508
                     /locus_tag="VW157_001932"
                     /pseudo
     CDS             1695..2508
                     /locus_tag="VW157_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          780 a          445 c          478 g          805 t
ORIGIN      
        1 tcaaatcgtt ctatagtaaa atgaaacaag aacaggacaa attgatcagg acagtcaaat
       61 cgatttctaa caatgtttta gaagtagggg tgtactattc tagtttaaat ccactatatt
      121 tggggagtga tagaaaagcc cttcataagc cagtctactt gttcaggtgc gagagctttg
      181 acatcctttt ctgtactggg ccaagtcagt tttccgttct caaagcgttt atatagtatc
      241 caaaatcctt gaccatccca gtaaagggct ttaaagcggt ctttacgtcc accacaaaag
      301 agaaagactt gaccggagaa aggatccaat tcaaagtggg ttttaacgag ataagccagt
      361 gaatcgattc cttgtatcat atctgtcttg ccacaaacaa ggtgaacttg acctaaatca
      421 cttagttgaa tcatcatagt acaatacttt tcctccgata attatttttt atctagtata
      481 ctggaagttg gggaattagg atagatacct tgttatgacg cgcttacgta acttgtaact
      541 agctgcctag tttgatcttt gcttcttcat tgattagcag cagatttcaa aatgataaaa
      601 acgtataata gtgggtgtag aaactcttgc tactatgcgt tttattatgg aaagacttat
      661 tggactttct ctcaaatcga gtttttactc aattttctta cttgattggg attgaaattc
      721 caattaattt ctctgagtag agtgtcttga tattggcttc atcaacagag gccttatcaa
      781 ttttacgttt caagaaaaat tcttgaatgg tttcgatttc aggctcacga atagcacggt
      841 gtttgtttga gatgaggatt tcatagtgaa gcggagcttg ggtaaaaata acatctgtat
      901 tccctgcaga ataaacctca acaagggttg catcggtact ttctagctga ctttttacaa
      961 gttgcgagtg tgagtttgtc gtattgataa gcttcataat atttcctccg attttctaat
     1021 tctattatag cactttttga ataaagtcgc ttgatttata ctcaatgaaa ataaaagagc
     1081 aaactaggaa gctagccgca ggctatactt gagtatggta aggcgacgct gatgtggttt
     1141 gaattttatt ttcgaagagt attagtcaat cttatgctgt tttttccaag attcaatggc
     1201 ccatttatgg ctaccacgtt taaggttttt gatagcctcg tcaataggga accaggcaat
     1261 atgattaaag ttttctagtg gcttttgtac ttctttgaaa ggagttgctt catagaggta
     1321 ggcaggattg tagtagtagg tatcacgatg acgagaatag aaatattcgt cagcttgtcc
     1381 gtaataggta ccaatttctg ctgtgaaacc aagctcttca atcaactcac gctttagggc
     1441 ttcctgatga ttttcacctg cttcaatttc tccacatggt aggaaccaag caccatttgg
     1501 ttcttgaaca agaacaattt gtttttgttc aggattaggg ataactgcat atacgccata
     1561 gcgagcaata tagtctgtat tcgctttttt tctccgaaag ttgggtttgc cattgcattt
     1621 tcctcattat ctagtatcgt tattattata gtgaaatgaa ccaaaaatag tacacaatgt
     1681 ggtataatct tcttatggca tattcaatag attttcgtaa aaaagttctc tcttattgtg
     1741 agcgaacagg tagtataaca gaagcatcac acgttttcca aatctcacgt aataccattt
     1801 atggctggtt aaagctaaaa gagaaaacag gagagctaaa ccaccaagta aaaggaacaa
     1861 aaccaagaaa agttgataga gatagactta aaaactatct tactgacaat ccagacgctt
     1921 atttgactga aatagcttct gaatttggct gtcatctaac taccatccac tatgcgctca
     1981 aagctatggg ttacactcga aaaaaagaac cacacctact atgaacaaga cccagaaaaa
     2041 gtagccttat ttcttaagaa ttttaatagt ttaaagcacc tagcacctgt ttagattgac
     2101 gaaacaggat tcgatactta tttttatcga gaatatggtc gctcattaaa aggtcagtta
     2161 ataagaggca aagtatctgg aagaagatat cagagaattt ctttggttgc aggtctaaca
     2221 aatggtgagt taatcgctcc aatgacttac gaagagacga tgacgagcga cttttttgaa
     2281 gcttggtttc agaagtttct cttaccaaca ttaaccacac catcggttat tattatggat
     2341 aatgcaagat tccatagaat gggtaagtta gaactcttgt gtgaagagtt tgggtataac
     2401 tttttacctc ttcctcccta ctcacctgag tacaatccta ttgagaaaac atgggctcat
     2461 atcaaaaagc acctcaaaaa ggtattacca agttgcaata ccttttat
//
LOCUS       DAVKJD010000125         2438 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKJD010000125 DAVKJD010000000
VERSION     DAVKJD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.125"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            134..1141
                     /locus_tag="VW157_001933"
     CDS             134..1141
                     /locus_tag="VW157_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9553179.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            1144..1344
                     /locus_tag="VW157_001934"
     CDS             1144..1344
                     /locus_tag="VW157_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9553180.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            1496..1678
                     /gene="rpmF"
                     /locus_tag="VW157_001935"
     CDS             1496..1678
                     /gene="rpmF"
                     /locus_tag="VW157_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9553181.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            1694..1843
                     /gene="rpmG"
                     /locus_tag="VW157_001936"
     CDS             1694..1843
                     /gene="rpmG"
                     /locus_tag="VW157_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9553182.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
BASE COUNT          812 a          462 c          447 g          717 t
ORIGIN      
        1 gagcacaata taatctcacc ttttaaaaga aattattggg ctgaaagttt ttagtgatta
       61 tttttcaact ttctattgac tttataaatt aaagtgatat catttagata tcaaataaca
      121 ggaggtcatt cttatgactg aaaaactaat caattcaaaa ccaaatggtg tattcgcatt
      181 gattctcatt gagttgacaa tcgtacttgg tatctttata tttataatgg gggttggttc
      241 ggaaaacatt tttggaatta ttatcggacc tttactaatc gtaattgcag gtctagctca
      301 tgctggttta aaagttgtca aacctcagga agctctggtt ctgacactat ttggtaatta
      361 tacaggtacc atcaaagaac ctggttttta ctttgtcaat cccttcagcg tagcagtcaa
      421 ccctgcaaac cacactcgac ttggacaaag tggtgatgtt agcacaaaat ctcctttttt
      481 aggagctaaa tcatcaaatg acaatgatgt aaatcttgaa attggcaaga aacacatttc
      541 cctcaaagtc atgaccttga gcaattctcg tcaaaaaatc aatgattgct taggaaaccc
      601 tgtagaaatc ggtatcgcgg taacttggag agttgtcgat accgctaagg cagtcttcaa
      661 cgttgataac tacaaagaat atctttcatt gcaatgtgat agcgccctcc gtaatattgt
      721 ccgcatctat ccttacgatg tgtctcctaa tgtggatact acgggtgacg ggcaagcaga
      781 tgaaggtagt ctccgtggct ctagcgaaat tgttgctaac cgtattcgtg aagaaatcca
      841 aagtcgtgtt gaggatgctg gcttggaaat ccttgaagca cgtatcactt acctagctta
      901 tgctccagaa attgctgccg ttatgcttca acgccaacaa gcatctgcca ttattgatgc
      961 acgtaagatg attgtagatg gtgctgtagg aatggttgaa atggcactag aacggctcaa
     1021 tgaaggggaa ttggtagaac ttgacgaaga acgaaaagct gccatggttt caaatctcct
     1081 tgtcgttctt tgtggcaatc atgatgcaca accaattgtc aacacaggaa gtctttacta
     1141 aagatggctg aagctaaaaa aaagcagatc ccccttcgac tctcaacaaa gttatacgct
     1201 gcactcgcat catgggcaga agacgacttt cgttcagtca atgggcaaat agaatatctc
     1261 ctcacagagt gtgtcaagca acggaaaaaa gacggaaaat acgtatcaga aaccattgac
     1321 gaaccatttg agattgatat ttaacacctt ctcctgtctg ccgtgtcgga taggagaatt
     1381 ttttcatctt tttttgtcaa gtaactttac tttacaaaaa aaatgtgtta tcctagtatg
     1441 gttgatgaaa atcagtagat tgaatcgaat ataaatacct aaaggagaaa tcaaaatggc
     1501 agtacctgca cgtcgcactt caaaagcgaa gaaaaacaaa cgtcgtacac actacaaagt
     1561 aacagctcca tctgtaaact ttgacgaaac tactggagat tactcacgtt ctcaccgtgt
     1621 atcacttaaa ggatactaca aaggacgtaa aatcgctaaa gctgcatcag ctgaataata
     1681 gaagggagat actatgcgcg taaatattac acttgaacac aaagaatctg gtgaacgctt
     1741 gtaccttact tctaaaaaca aacgtaacac tccagaccgc cttcaattga agaaatactc
     1801 accaaaactt cgcaaacacg ttgtgtttac agaagtgaag taagcattca atacgaatca
     1861 atacagaaga aaaacgcttg atcagcgttt tttctttttg ttaatttcac tatattgcaa
     1921 gggaaatcat tcgaatgact acattattga aataagaata cggattcgga caattaaaag
     1981 caagaggaac tcaaatgtaa gttggggttc cttttttgta tatttttcaa ataacgcctc
     2041 tacactattt gtagcaaatt caccaactac agttgtatct tagttaaaat aagttagaat
     2101 atgtaagtga gtaccagata taccaagaca tcgtcaccat ctaaggtata ttcaaaatac
     2161 aaaagttgac caactagatt tctgaatatc cttatatatc cattcttaaa attggtttaa
     2221 atagcgtagt cttttaaact agttttgaga atccaaaaaa tcttcctaca tatgtaagaa
     2281 gattttttag ttcagaatga ttagatttag ctaatggata cctatcctac ccactcaccg
     2341 ttagcattga ctcgatagcc atcgggtgta gtcgtgttta ctaataaatc tcctgaatta
     2401 taggtatagt accattgacc agaaactgta aaccaacc
//
LOCUS       DAVKJD010000126         2424 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKJD010000126 DAVKJD010000000
VERSION     DAVKJD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.126"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..347)
                     /locus_tag="VW157_001937"
     CDS             complement(<1..347)
                     /locus_tag="VW157_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553183.1"
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFNPIISNTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IRKDFDIKLTNEY"
     gene            complement(344..697)
                     /locus_tag="VW157_001938"
     CDS             complement(344..697)
                     /locus_tag="VW157_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9553184.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGAYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLAGAAGRIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
     gene            complement(753..1655)
                     /locus_tag="VW157_001939"
     CDS             complement(753..1655)
                     /locus_tag="VW157_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553185.1"
                     /translation="MIRKPIIFLLMLPIWGLWIELHLLVSNLQLNLEIPFDFVVSTSL
                     TFFVLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTNTLKDKIQKSELSYSDLMSLQLYDAMNQSTFLTDSELFEDILSKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            complement(1863..2237)
                     /locus_tag="VW157_001940"
     CDS             complement(1863..2237)
                     /locus_tag="VW157_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU9553186.1"
                     /translation="MELVLPNNYVALEQEEMMYFDGGFSIPRWPVATAINIAFNDVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          852 a          455 c          319 g          798 t
ORIGIN      
        1 ttatattcgt tagtcaattt aatatcaaaa tctttcctaa ttaaatgaaa gattgctaac
       61 tctaggataa aaacacttat taatacggta taagtcaaaa aactttctgg gaaaaactta
      121 ttgattaaaa tggggaaact cattaacaga atcgaattta tggctagaca gatagatact
      181 attttatttt tcacagaata tagaggtagt gatactattg agtagataaa agctaaaaaa
      241 ataagcaaaa aatactccgt gttactgatt atcggattaa atgataaaaa tatatacatc
      301 caacatacga taatgtgtat ttttcgaatc ttatctacat tactcatctt actgtcgcag
      361 atatgaatcc atcccaagga taagggttcc cgctaatatc acaacctcta ttaagagcac
      421 catatccaat acagtaagct attcttcctg ccgccccagc caatacacct gctgctgcac
      481 caataagcca acctccaagg cctccaactt taatatagtt tattagtttt ttggttaaaa
      541 ctgcagctcc agctaatgct ataaaagttc ctggactcat agctaaagct gcgcaaattc
      601 cttgacaagc acgcttgctt aaataagctc ccccatcaag atacatcatc tcttcttgct
      661 caagagcaac ataattattt ggtaatacga gttccatttg ttttctcctc cttagttgaa
      721 attgtttcat ttgttttaca aataggacga acttaaacta tctttttatt actcagtaat
      781 aatttccgag atttcatttt atgatactct ctattcatac agaaatcaaa attttgcctt
      841 aaccaacttg tataatagaa aaaatctaat tgatttattc tgatgagctg tattctaatt
      901 ttatcaacct tttcttgcca gttgttattg ttactaatag aaatctcata cagcagtaac
      961 tcgacaagac ctgtacacag aagggtgtta tgagaatctt caattttact cagaatatcc
     1021 tcaaagagtt cactatccgt taaaaaggtg gattggttca tcgcatcata cagctgaaga
     1081 gacattaagt cagaatagct taattctgat ttttgtatct tatcctttaa cgtatttgtc
     1141 aaatcacttt caaacgaatc ttctttttcc catttatttt gattgaaaag aaattccgtc
     1201 ttattaaaaa cgagatgatt gatatccatt atagtccaga aaaatattgt agtaagcatg
     1261 atgattgcaa aaggaatttt taaaataaat actaggataa tagcaatttc aagaataaaa
     1321 ctccatataa tcctagatct taaatcatca ggcgacaaac ttttacgatg gaatgaaaaa
     1381 tagggcgaga acctcacatt tactttcttc cttcctataa aaataaaggg aaaaatcgta
     1441 ataagagctt ctttcttgta caaatctttc aatgcgtata gtatgtctaa aactattttt
     1501 gataatatca aaacaaaaaa agtaagactc gtactaacca caaaatcaaa gggaatttct
     1561 agattcaatt gcaagttact cactaacaaa tgcaactcta tccacaagcc ccaaataggg
     1621 agcattaata aaaaaatgat tggtttacgt atcataattt tacagtgtcc ttatcatttt
     1681 cttaattttg gataaattat cattgctatg attaaaatca aaaaaacgac attcatccaa
     1741 ataatcaact tttttatttc ttcgctaaca aaatcatagg ctaaaaaagt tatggatgat
     1801 ataaatagac atacaaaaaa aataataaca aaaatttttt ccattttaat acccatatct
     1861 agttaccaca aggcgtttaa atttgggtct ctcctattta catcattgtt ggcccagatt
     1921 gttgtaatag catcgccaat tgacatccaa gctgcaaatc cattaatagc agacagtgca
     1981 aatcctgcca ctctatttgc aactcgtacc ccaacatatc ttgcagctgc tccagcaatt
     2041 gcgcttgtaa ctcgccgcaa accataatta cgaatataat ttctaactag actgattgct
     2101 cctccaccta agacatcatt aaaagctata ttaatggctg ttgcaacagg ccatctcgga
     2161 atagaaaatc ccccatcaaa atacatcatc tcttcttgct caagagcaac ataattattt
     2221 ggtaatacga gttccattta ttttctcctt ttttattaag catagttagg atggtttcct
     2281 aacaagtgat tgtcgactgt gtttatagta cttagttacc taaataagat atcctttctc
     2341 taccttattt gtttttatag gttacctaag gtttaaaacc aattattgct tgaaacttaa
     2401 cagttctaaa ccactagcaa taaa
//
LOCUS       DAVKJD010000127         2289 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKJD010000127 DAVKJD010000000
VERSION     DAVKJD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.127"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..652)
                     /locus_tag="VW157_001941"
     CDS             complement(<1..652)
                     /locus_tag="VW157_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9553187.1"
                     /translation="MGQLHFITKLLDIKDTNIQIIDVVNRDSHKEIIAKLDYDAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDEYA
                     FTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPYY"
     gene            complement(1266..2258)
                     /locus_tag="VW157_001942"
     CDS             complement(1266..2258)
                     /locus_tag="VW157_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9553188.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIISANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
BASE COUNT          748 a          383 c          347 g          811 t
ORIGIN      
        1 catagtaagg actaaacata tccatcgtaa tgattttcac ttgacaacga acggctctat
       61 cgtagcgaag aaagtgattt cggatgacag cttgtgttct gccttcaaga acagtgataa
      121 tattaagatt atcaaaatct tgcgcaatga aactcatctt tcccttagtg aaggcatact
      181 catcccaaga cataatcttt ggaagccgag aaaaatcatg ctcaaagtga aagtcattga
      241 gcttgcgaat gacagttgaa gttgaaatgg ccagctgatg ggcaatatca gtcatagaaa
      301 ttttttcaat taacttttga gcaatttttt ggttgatgat acgagggatt tggtgatttt
      361 tctttaccag gggagtctca gcaaccatca tttttgaaca gtgatagcac ttgaaacggc
      421 gttttctaag gagaattcta gaaggcatac cagttgtttc gaggtaaggg atcttagacg
      481 gtttttgaaa gtcatatttc ttcattagac ttccacaatc agggcaagat ggggcgtcgt
      541 agtccagttt ggcgatgatt tctttgtgtg aatccctatt gacgacatct ataatttgga
      601 tatttgtgtc tttgatatcg agtagttttg tgataaaatg taattgtccc atatgaatct
      661 ttctaatgag ttgtttgatc gcttttcatt atagatcata tgggactttt tttctacaat
      721 aaaataggct ccataatatc tataggggat ttacccacta caaatattat agagcccaaa
      781 aatgctctga aacactgatt tcaaagcatt ttttgttagt ttaaaattac tcccattctt
      841 cttttccaaa cgtacaatat atccaaaacc attcaaaata ctagattcta ttttttataa
      901 tatcactaaa tccacctaat tataggacgt tttcagattt ttagtcccgt cccagtccca
      961 gtaccggaga aatattgttt taatataata tctctttttg tcttctaagc tcttaaaagc
     1021 aaaagaacaa gtaaagagtc aagacaagga taaaaagtcc atattagggc aaataaaaag
     1081 ctttaagaca gatgacaaat ctaagtcaaa taagaaagac catagcaaag gtgcagagag
     1141 ataaatattg gcggtcttcg gactgccttt atttttttat ccatttttca aatcaaattt
     1201 attcagacta tatatgcaca tatacactta aattcatata aaaacatggc ttgtaaaaaa
     1261 ttactttaat cacaataatc gcatttaaaa ttgtgatgtt tgcaagctaa attacggact
     1321 tcacttggaa gttttccctt gtatctttta taatagatag aaaatttgct ggcagatgaa
     1381 tatccaacag attctgctat ctcttttata ggtagttcag tgtttaaaag aagagtttca
     1441 gctacattca ttctttttct ttgagtgtac tctgtaatgc tttgacaata tttttcctta
     1501 aataaatttt ttaatttagt accactcatt ttagatattt tttcaagcgt gccttgattt
     1561 acattcgttg caaaatgatc atctaagaat cttgctacat cttcaagtgc tttatcatca
     1621 tcaatttcaa ttttatattt ttttctattt aagtatgtgt caattactat acttatccat
     1681 tcatttgcct ttgctttaaa gaaaaaatca gcggcaggag cgtccatctt acaatttaat
     1741 atttccattg ccactctttc taaggccttt gtaagtatta tttgattcgg ttgaagcaag
     1801 gttgaataaa aagattctgg attaatgtta atagatgcta aatgtttttc tattagctct
     1861 tttttaaaac caatggaaac agcaagataa caacaattct cgtgtaataa aaaaacaaaa
     1921 tcatctttta tattatcaaa atcaaaagta cataaagagt ttgcggtaat agtttgatac
     1981 ggattaaact tttctccgtt tgcactgata atgtaacttg aataaattga aacatagtct
     2041 gacatactat aagtgctatt ttgaactact tcctctttga tataaaaatc atgtatatcg
     2101 ataatgaagc tgcctccttc ataaaaccaa tataggcctt ctgcataagt tgaattctct
     2161 tttttccaac aaaatgtatg acctgcactt gaatacttct cattgttttt acattcatct
     2221 actttcatat aatcttttac aaaatcataa tatgacataa cacactatcc cttttagaca
     2281 atattccaa
//
LOCUS       DAVKJD010000128         2285 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKJD010000128 DAVKJD010000000
VERSION     DAVKJD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.128"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            191..529
                     /locus_tag="VW157_001943"
     CDS             191..529
                     /locus_tag="VW157_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553189.1"
                     /translation="MKKRINKKASVFLVASVFSFSFATTASVVNAVTAHPAPSPIIDV
                     WEYGVNDNYGYSNYFVSSPSNIGSKSSVTNFWGTVKAQDKKDYGWAYSSATKSWNDVR
                     LNAYWDYYRF"
     gene            595..>2285
                     /locus_tag="VW157_001944"
     CDS             595..>2285
                     /locus_tag="VW157_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9553190.1"
                     /translation="MKLKLCIIGFFFCLIATIGLVTISDTEIPIPLPIDGAFSIKGKS
                     NLSNNEIYEMVRDLSKTEKVTIYKPIVQSSGQLKYVNFDDVNNEQLKSAPIVGMYYTL
                     GKMDVDSLKPLTMTGLQTVYMAYPWYIGGILQFTGTLRILLMGSIYLTLLVVLFVVRT
                     RQIKEGVIRRSLGLPIYDLRREYGISLIFELIMMALLMISYSSFLGNGFFTYSSKLFF
                     SLLLTNFILFQIIDLITFVLFWLTIQIEKPIEIIKNKAKNKLIFVVWLAIISIIILVS
                     GVFLQETKSSQSSINIQIQNLVPWDTVKDWRRIEFLGIESNSTKNREVNDSDGQYLQI
                     VAALKNLDFLYIERSSAYVPDFMKTSHVIENFSKQLENDGITNPEINKELIYINQTGA
                     NLQNKVNGTNYHLLDNKIATIYIPEKWKENQKSIENTVVAEQFIGTNYTREQLAVQII
                     PDGEKIFYFNEDADNNLKMKDILPLANVADSKDNIVVVLDTDKMMENNKFSLASNILY
                     KSLFSPEAVKKINEMTVLLNFSMNPVDVYQIVKLKIQSLEHQILLSQILQKIIYS"
BASE COUNT          816 a          304 c          404 g          761 t
ORIGIN      
        1 gtaaacggat acatttagat gtgttcaatt ttaaaaggag gggtagttat gagatatttt
       61 aacgactcta gataccataa cggtgattaa aaacctaaat ataatcaatt catattaaag
      121 taatatggag ggactaaaaa gagactatta tttaatcttc atcaaatatt taaattcaag
      181 gagaagtaaa atgaaaaaaa gaattaataa gaaagcatct gtttttctag ttgcaagtgt
      241 tttcagtttt agctttgcta ctacagctag tgtcgttaac gcagtaacgg cacaccctgc
      301 tccaagtccc attattgatg tatgggaata tggtgtaaat gataactatg gttattcgaa
      361 ttactttgtt agttctccaa gtaatattgg atcaaaatct tcagtaacga atttttgggg
      421 aacagttaaa gctcaggata aaaaagatta tggatgggca tattcaagtg caacaaaatc
      481 ttggaacgat gttcgcttaa atgcatattg ggattattat agattttaaa aataatttat
      541 gaaaacaaga gacccaatta taagattggg tctcttgtaa tatggtaatt gcttatgaaa
      601 ttaaaattat gtatcattgg attcttcttt tgtttgattg ctacaattgg tctggtcact
      661 attagtgata ccgaaatccc cataccttta ccaattgatg gagctttttc tatcaaagga
      721 aaaagtaatc tttctaataa cgaaatatac gaaatggttc gtgacttatc aaaaacggaa
      781 aaggttacca tttacaagcc aatcgttcag agttcgggtc agttgaagta tgtaaatttt
      841 gatgatgtaa ataatgaaca attaaagtca gcaccaatag tagggatgta ttacacactt
      901 gggaaaatgg atgtagacag tttgaaacca cttacgatga cgggactaca aacagtctat
      961 atggcttatc cttggtatat aggaggaata ttacaattca ctggaacttt aagaatactg
     1021 ttaatgggtt caatttattt aactttatta gttgttttat ttgttgttag gacgaggcag
     1081 atcaaagaag gagtgatacg acgttctttg ggcttgccta tatacgactt aagaagagag
     1141 tatggcattt ctcttatttt tgaactgatt atgatggctt tattgatgat ttcttacagt
     1201 tcttttttgg gtaatggttt cttcacctat agttctaagt tatttttctc tctgctttta
     1261 acgaatttta tactatttca aatcattgat cttattactt ttgttttatt ttggttgacg
     1321 attcagattg aaaagcctat tgaaattatt aaaaacaagg caaaaaacaa gcttattttt
     1381 gtcgtatggc ttgctattat ttcgattatc atccttgtct caggagtttt tttacaagaa
     1441 acaaagagta gtcaatctag tattaacata cagatacaga atttagtacc atgggataca
     1501 gtaaaagact ggagaaggat tgagttcctt ggaattgaaa gtaactctac taaaaataga
     1561 gaagttaatg attcagatgg tcagtatcta cagatagttg ctgccttaaa aaacttagat
     1621 ttcttatata tagaacggtc ttcagcatat gttccagatt tcatgaaaac ttcacatgtt
     1681 atagaaaatt tttctaaaca attagaaaat gacgggataa cgaatccaga aataaataaa
     1741 gagttaattt atatcaacca aacaggtgct aacctacaaa ataaagtgaa tggaacaaac
     1801 tatcatcttt tagataataa gatagcaacc atctatattc ctgagaagtg gaaagaaaac
     1861 cagaaatcta ttgagaatac agttgtagca gaacaattta ttggaacaaa ctatacaaga
     1921 gaacagcttg cagttcaaat aattcctgat ggggaaaaaa ttttttattt taatgaggat
     1981 gctgataata atcttaaaat gaaagatatt ttaccgctgg caaatgtagc agatagcaaa
     2041 gataatatag ttgttgtgtt agatacggac aaaatgatgg aaaataataa gttttctttg
     2101 gctagtaaca tactatataa atctcttttt agtcctgaag cggtaaaaaa aataaatgag
     2161 atgactgtcc tgttgaattt ctcaatgaat ccagtagatg tttaccaaat tgtgaaatta
     2221 aagattcaat ccttagagca ccaaatttta ctttcacaaa tcttgcagaa gataatttac
     2281 agtat
//
LOCUS       DAVKJD010000129         2257 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKJD010000129 DAVKJD010000000
VERSION     DAVKJD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.129"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            89..538
                     /locus_tag="VW157_001945"
     CDS             89..538
                     /locus_tag="VW157_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9553191.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQKNKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LEILQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYESVLPVRQIFD"
     gene            695..1105
                     /locus_tag="VW157_001946"
     CDS             695..1105
                     /locus_tag="VW157_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9553192.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKRIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            1221..>2257
                     /gene="pavB"
                     /locus_tag="VW157_001947"
     CDS             1221..>2257
                     /gene="pavB"
                     /locus_tag="VW157_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9553193.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDG"
BASE COUNT          694 a          436 c          501 g          626 t
ORIGIN      
        1 aacatctata cggcaaggcg acgttgacgc ggtttgaaga gattttcgaa gagtataaga
       61 aaaaatcagt cccctaaagg agcagattat gaagttattg tctatcgcca tttctagcta
      121 taatgcagca gcctatcttc attactgtgt ggagtcgcta gtgattggtg gtgagcaagt
      181 tgggattttg attatcaatg acgggtctca ggatcagact caggaaatcg ctgagtgttt
      241 agctagcaag tatcctaata tcgttagagc catctatcag aaaaataaat gccatggcgg
      301 tgcggtcaat cgtggcttgg cagaggcttc tgggcgctat tttaaagtag ttgacagtga
      361 tgactgggtg gatcctcgtg cctacttgaa aattcttgaa atcttgcagg aacttgagag
      421 caagggtcaa gaggtggatg tctttgtgac caattttgtc tatgaaaagg aaggtcagtc
      481 tcgtaagaag agtatgagtt acgagtcagt cttgcctgtt cggcagattt ttgactagga
      541 ccaggtcgga aatttctcca aaggccagta tatcatgata cactcgctga tttatcggac
      601 agatttgttg cgtgctagcc agttctaact gcctgaacat actttttatg tcgatgatct
      661 ctttgtcttt acgccccttc agcaggtcaa gaccatgtac tatctgcctg tcgatttcta
      721 tcgttatttg attgggcgtg aggaccagtc tgtcaatgag caagtgatga ttaagcgcat
      781 tgaccagcaa ctcaaggtca atcgactctt ggtagaccag cttgatttgt cccaagtgag
      841 tcatcccaaa atgcgagaat atctgctgaa tcatattgaa atcacgacgg tgatttccag
      901 taccctgctc aaccgatctg aaacagcgga gcatctggca aaaaaacgcc aattgtggac
      961 ctatattcag cagaaaaatc cagaagtctt tcaggctatt cgtaagacca tgttgagccg
     1021 tttgaccaaa cattctgtct tgccagatcg caaactgtcc aatgtcgtct atcaaatcac
     1081 caaatctgtt tatggattta attaatataa gtgttttata agagggattt aagaaaaatt
     1141 ttaacttttt cttagtcctt tttaatttca ggagattata ctagagtcat caaataaaga
     1201 aagactctaa ggagaatcct atgaaattca atccaaatca aagatatact cgttggtcta
     1261 ttcgccgtct cagtgtcggt gttgcctcag ttgttgtggc tagtggcttc tttgtcctag
     1321 ttggtcagcc aagttctgta cgtgccgatg tggtcaatcc gaccccaggt caagtcttac
     1381 ctgaagagac atcgggaacg aaagagggtg acttatcaga aaaaccagga gacaccgttc
     1441 tcactcaagc gaaacctgag ggcgttactg gaaatacgaa ttcacttccg acacctacag
     1501 aaagaactga agtgagcgag gaaacaaact cttctagtct ggatacactt tttgaaaaag
     1561 atgaagaagc tcaaaaaaat ccagagctaa cagatgtctt aaaagaaact gtagatacag
     1621 ctgatgtgga tgggacacaa gcaagtccag cagaaactac tcctgaacaa gtaaaaggtg
     1681 gagtgaaaga aaatacaaaa gacagcatcg atgttcctgc tgcttatctt gaaaaagctg
     1741 aagggaaagg tcctttcact gccggtgtaa accaagtaat tccttatgaa ctattcgctg
     1801 gtgatggtat gttaactcgt ctattactaa aagcttcgga taatgctcct tggtctgaca
     1861 atggtactgc taaaaatcct gctttacctc ctcttgaagg attaacaaaa gggaaatact
     1921 tctatgaagt agacttaaat ggcaatactg ttggtaaaca aggtcaagct ttaattgatc
     1981 aacttcgcgc taatggtact caaacttata aagctactgt taaagtttac ggaaataaag
     2041 acggtaaagc tgacttgact aatctagttg ctactaaaaa tgtagacatc aacatcaatg
     2101 gattagttgc taaagaaact gtcgaaaaag ctgttaaaga taacgttaaa gacagtatcg
     2161 atgttccagc agcctactta gaaaaagcca agggtgaagg tccattcaca gcaggcgtca
     2221 accatgtgat tccatacgaa ctcttcgcag gtgatgg
//
LOCUS       DAVKJD010000130         2053 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKJD010000130 DAVKJD010000000
VERSION     DAVKJD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.130"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            22..939
                     /gene="adcAII"
                     /locus_tag="VW157_001948"
     CDS             22..939
                     /gene="adcAII"
                     /locus_tag="VW157_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9553194.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            947..>2053
                     /gene="phtD"
                     /locus_tag="VW157_001949"
     CDS             947..>2053
                     /gene="phtD"
                     /locus_tag="VW157_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEU9553195.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEERIA
                     RIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPSPQP"
BASE COUNT          677 a          399 c          434 g          543 t
ORIGIN      
        1 taaatggtta aggagttgtt catgaagaaa caaaatttat ttttagtcct gttaagtgtc
       61 tttcttttgt gcttgggggc ttgtggtcaa aaggaaagtc agacaggaaa ggggatgaaa
      121 attgtgacca gtttttatcc tatctacgct atggttaagg aagtatctgg tgacttgaat
      181 gatgttcgga tgattcagtc aagtagtggg attcactcct ttgaaccttc ggcaaatgat
      241 atcgcagcca tctatgatgc agatgtcttt gtttaccatt ctcatacact cgaatcttgg
      301 gcaggaagtc tggatccaaa tctaaaaaaa tccaaagtga aggtcttaga ggcttctgag
      361 ggaatgacct tggaacgtgt ccctggacta gaggatgtgg aagcagggga tggagttgat
      421 gaaaaaacgc tctatgaccc tcacacatgg ctagatcctg aaaaagctgg agaagaagcc
      481 caaattatcg ctgataaact ttcagaggtg gatagtgagc ataaagagac ttatcaaaaa
      541 aatgcgcaag cctttatcaa aaaagctcag gaattgacta agaaattcca accaaaattt
      601 gaaaaagcga ctcagaaaac atttgtaaca caacatacag ccttttctta tctagcgaag
      661 agatttgggc ttaatcaact tggtattgca ggtatctctc ctgaacaaga accaagtcca
      721 cgacaactaa cagaaattca ggaatttgtt aagacctata aggttaaaac gatttttaca
      781 gaaagtaacg cttcttcaaa agtagctgaa actcttgtca aatcaacagg tgtgggtctt
      841 aaaactctga atcctttaga gtcagaccca caaaatgaca agacctattt agaaaatctt
      901 gaagaaaata tgagtattct agcagaagaa ttaaagtgag gaaagaatga aaatcaataa
      961 aaaatatcta gcaggttcag tggcagtcct tgccctaagt gtttgttcct atgaacttgg
     1021 tcgtcaccaa gctggtcagg ttaagaaaga gtctaatcga gtttcttata tagatggtga
     1081 tcaggctggt caaaaggcag aaaatttgac accagatgaa gtcagtaaga gagaggggat
     1141 caacgccgaa caaattgtta tcaagattac ggatcaaggt tatgtgacct ctcatggaga
     1201 ccattatcat tactataatg gcaaggttcc ttatgatgcc atcatcagtg aagaacttct
     1261 catgaaagat ccgaattatc agttgaagga ttcagacatt gtcaatgaaa tcaagggtgg
     1321 ctatgtcatt aaggtagacg gaaaatacta tgtttacctt aaagatgcgg cccatgcgga
     1381 caatattcgg acaaaagaag agattaaacg tcagaagcag gaacgcagtc ataatcacaa
     1441 ctcaagagca gataatgctg ttgctgcagc cagagcccaa ggacgttata caacggatga
     1501 tgggtatatc ttcaatgctt ctgatatcat tgaagatact ggtgatgctt atatcgttcc
     1561 tcacggcgac cattaccatt acattcctaa gagtgatttg tctgccagtg aattagctgc
     1621 tgcccaagcc tattggaatg ggaagcaggg atctcgtcct tcttcaagtt ctagtcataa
     1681 tgcaaatcca gctcaaccaa gattgtcaga gaaccacaat ctgactgtca ctccaactta
     1741 tcatcaaaat caaggggaaa acatttcaag ccttttacgt gaattgtatg ctaaaccctt
     1801 atcagaacgc catgtagaat ctgatggcct tgttttcgac ccagcgcaaa tcacaagtcg
     1861 aacagccaga ggtgtagctg tccctcatgg taaccattac cactttatcc cttatgaaca
     1921 aatgtctgaa ttggaagaac gaattgctcg tattattccc cttcgttatc gttcaaacca
     1981 ttgggtacca gattcaagac cagaacaacc aagtccacaa ccgactccgg aacctagtcc
     2041 aagtccgcaa cct
//
LOCUS       DAVKJD010000131         2013 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKJD010000131 DAVKJD010000000
VERSION     DAVKJD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.131"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..1022
                     /locus_tag="VW157_001950"
     CDS             <1..1022
                     /locus_tag="VW157_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9553196.1"
                     /translation="NKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAF
                     GQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELN
                     GKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLW
                     RDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTS
                     KFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGD
                     DKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNA
                     CKNAK"
     gene            1012..>2013
                     /locus_tag="VW157_001951"
     CDS             1012..>2013
                     /locus_tag="VW157_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9553197.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     "
BASE COUNT          705 a          288 c          361 g          659 t
ORIGIN      
        1 aaaacaaatt ttttctaata gctattttag ctatgtgtat agtttttagc gcttgttctt
       61 ctaattctgt taaaaatgaa gaaaatactt ctaaagagca tgcgcctgat aaaatagttt
      121 tagatcatgc tttcggtcaa actatattag ataaaaaacc tgaaagagtt gcaactattg
      181 cttggggaaa tcatgatgta gcattagctt taggaatagt tcctgttgga ttttcaaaag
      241 caaattacgg tgtaagtgct gataaaggag ttttaccatg gacagaagaa aaaatcaaag
      301 aactaaatgg taaagctaac ctatttgacg atttggatgg acttaacttt gaagcaatat
      361 caaattctaa accagatgtt atcttagcag gttattctgg tataactaaa gaagattatg
      421 acactctatc aaaaattgct cctgtagcag catacaaatc taaaccttgg caaactttat
      481 ggagagatat gattaaaatt gattcaaaag ccttaggtat ggaaaaagaa ggtgatgagt
      541 taatcaaaaa tactgaagct cgtatatcca aagaattaga aaaacatcca gaaatcaaag
      601 gaaaaatcaa aggaaaaaaa gtattattta ctatgattaa tgctgcagat acatcaaaat
      661 tctggattta tactagcaaa gatccaagag caaattattt aacagattta ggtctagttt
      721 tccctgaatc attaaaagaa tttgagagtg aagatagttt tgcaaaggaa atttctgcag
      781 aagaagcaaa taagataaat gatgctgatg taatcataac ttatggtgat gataaaactc
      841 ttgaagcttt acaaaaagat cctcttttag gtaaaataaa tgcaattaaa aatggtgccg
      901 ttgctgtaat tccagataat acaccgttag cagcctcatg cactccaaca ccactttcaa
      961 taaactatac tattgaagaa tacctaaatc ttttaggaaa tgcatgcaaa aatgcgaaat
     1021 aaaaaacaaa taaacctagg cataattttt gtaatctgcc taggtcttct tattacaata
     1081 tttttgtcat taaagcttgg aacaaaagaa attaatatca gagatttttt agcagctttt
     1141 ggaatgggca atacaaatga tgattttatt aaatcaatta tatataaaag aatacctaga
     1201 actatttttg caattttagc aggttctagt cttgccataa gcggtgtatt gatgcaatca
     1261 gttactagaa acccaatagc tgatccaggt atactcggta taaacacagg agcaagtctt
     1321 agtgtagtaa ttggtctttc ttttttagga atttcatcaa gcataagcca tataagtttt
     1381 gcaatcattg gtggcttagt aagtgcaatt tttgtatacg cgattgctgt aagcggaaaa
     1441 gcaggtctta cccctataaa acttgcctta tcaggaactt gtgttagtat ggctttaagc
     1501 agttttgtaa gttttttaat tttaccgaat aataacgtct tagacaaatt tagattttgg
     1561 caaataggta gccttggagc agctacatta tcttctatat ctacactact accttttata
     1621 attttaggtc acttgatagc tatatttatt tcatcagatt taaacgcttt agctatgggt
     1681 gatgaaatgg ctgttggtct tggagttaat gttaatagga taagatcact tgcaataatt
     1741 gcaagtgtgc ttttatgttc aagtattact gcaattggtg gacctattgg cttcgtaggt
     1801 cttatagttc ctcacttttg tggcttattt ataagcaaag atatacgcac aatgaccatt
     1861 tcttcatctt ttataggtgc agagctcttg cttatatgtg atataatcgg ccgtatgtta
     1921 ggtaaaccag gtgaaattga agtagggata attactgcaa taatcggggg tccagtactt
     1981 atttatgtaa ctatgaaaaa tagaggggtt aat
//
LOCUS       DAVKJD010000132         1954 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKJD010000132 DAVKJD010000000
VERSION     DAVKJD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.132"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..992
                     /locus_tag="VW157_001952"
     CDS             <1..992
                     /locus_tag="VW157_001952"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEU9553198.1"
                     /translation="SETLTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPV
                     LAPFYFTFFGMMVADLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAIWGG
                     IYGSFFGYELPFHLISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYN
                     SGFAWCVILLGLLFIAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSGIGA
                     GLFNLYNISSYIGDLVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVLFIL
                     LHAINIFLSLLSGYVHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            1008..1484
                     /locus_tag="VW157_001953"
     CDS             1008..1484
                     /locus_tag="VW157_001953"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEU9553199.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            1518..>1954
                     /locus_tag="VW157_001954"
     CDS             1518..>1954
                     /locus_tag="VW157_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553200.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNY"
BASE COUNT          621 a          265 c          392 g          676 t
ORIGIN      
        1 aatcggaaac attgacggat aatcaagatg aaatacctat caaattaacg aatcattctt
       61 taattgaacc atttgaatta ttgacagaaa tgtatgctct gcccaaatat tatgagaaag
      121 atcctacacc tgtattagca ccattttact ttacattttt tggaatgatg gttgctgatt
      181 taggctatgg tttactattg tttttaggaa caatgttagc attaaaaatt tttcatctac
      241 cttcagcaac taagagattt ttaaaattct ttaatatatt aggggtagcc gttgcaattt
      301 ggggtggaat ctatggctca ttttttggat atgagttgcc atttcatctg atatctacaa
      361 cctctgatgt catgactata ttagtagtgt cagttgtgtt tgggtttatt acagtatttg
      421 caggtttgtt agcttcagga ctacaaaaag taagaatgaa gaaatatgca gaagcatata
      481 attcaggatt tgcgtggtgt gttattctgc ttggcttgtt atttattgct gttggaatgt
      541 tgatgcctga tatgagaccg ttatttgtat tagggaaatg ggtatctatt tttaatgctg
      601 tggggatttt gattgtttct attattcaaa ccaaaagctt gtcaggtatt ggagcaggat
      661 tgtttaatct atataacatt tcatcttata taggtgattt agttagtttc actcgattga
      721 tggcattagg attatctgga gcaagtatag catcagcttt caatttaatt gttggtttgt
      781 ttccgggaat attggctaaa ctgacaattg gattagtatt attcattctt ttacatgcga
      841 tcaatatttt tctatcgtta ctatcaggat atgttcatgg agcacgtctg atatttgttg
      901 aattttttgg taagttttat gagggtggag gaaaaccatt tcaacctttg aaggcttctg
      961 agaaatatat taaggttatt acaaagaatt aatggaggat atatataatg gaacatttag
     1021 caacttattt ttcaacctat ggaggagctt tcttcgctgc attgggaatt gtattggcgg
     1081 ttggattaag cggtatgggg tctgcttatg gagttggtaa ggctgggcaa tctgccgcag
     1141 ctttactgaa agaacagcct gaaaagtttg cctcagcttt gatattgcaa ttattgcccg
     1201 gaacacaagg attatatggt tttgttattg gaattttaat ttggttgcaa ttaactccag
     1261 aacttccttt agaaaaaggc gttgcttatt tctttgtagc tcttccaatt gctattgtag
     1321 gatacttttc agctaagcat caaggaaatg tagcagtagc gggaatgcaa atcttggcta
     1381 aaagaccaaa agaattcatg aagggagcaa ttttagctgc catggtagaa acctatgcaa
     1441 ttcttgcttt tgtcgtatca ttcattttga cccttcgtgt ataagaaata aatttgcaat
     1501 tcaaaggagg tgtctaaatg agcaatttag aaaacttacg agagtctgtt attgaacaag
     1561 ctcatgaaaa agggcgtatg aaattattgg attccaaaaa gaagattgat gatgaatttg
     1621 aaatgcaaaa gtcgctcatt ataaagaaaa aagaagctga acatgaacga aagttaaaag
     1681 aattgcaaca gaaatatcaa ataatttttc aacaattaaa aaataaggaa cgccaatcaa
     1741 cgttagtatc aaaacagaaa atattaaaag aactttttca atctgcttta ctagaaatgg
     1801 aatcttggag tgcagataaa gaaatggagt tcatctatcg aattctggaa cgatattcac
     1861 aacaagaggt catagtaacc tttggggaac ggactttagc taaattcaat ttggaacaat
     1921 tagagaaatt gaaattctct tttccaaatt attt
//
LOCUS       DAVKJD010000133         1819 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKJD010000133 DAVKJD010000000
VERSION     DAVKJD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.133"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..259
                     /locus_tag="VW157_001955"
     CDS             <1..259
                     /locus_tag="VW157_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553201.1"
                     /translation="FLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIG
                     LLPIFQYMVNSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            269..967
                     /locus_tag="VW157_001956"
     CDS             269..967
                     /locus_tag="VW157_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553202.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAIVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            964..1611
                     /locus_tag="VW157_001957"
     CDS             964..1611
                     /locus_tag="VW157_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9553203.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(1698..>1819)
                     /locus_tag="VW157_001958"
     CDS             complement(1698..>1819)
                     /locus_tag="VW157_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9553204.1"
                     /translation="IEKLGKENFFHKAYEKTLHPTYGDAAKADDLVVEGGKVD"
BASE COUNT          558 a          220 c          360 g          681 t
ORIGIN      
        1 ctttttatcc ttacttacta ttagcagttt ttcttggttt aaaaaatcaa acatgagctt
       61 agtatttctg tttacttttt tatttgtaga atccttattc tggatttatc agttggacaa
      121 tgggataatt ggattattgc caatttttca gtatatggta aattccaatc cgtatgcatt
      181 gatttattgg cttacattac tatctatcat aattccattg actgtatttt ctgttcatag
      241 aaactggagg agagtgtaaa agttggaaat gggaaagtta agtagtcaca tgtggaggtt
      301 gaatcagata atctatacca agtacttttg gggttatgtt cttttttgga tattgatttg
      361 tttaggatta tggtattggt tagaaggaaa tgatagactt gttatagaaa ttttaaaagg
      421 gcctaatctg agtcaaaact cttttttagt cttatctata tggttgcttc attggtttat
      481 tattcataca ttttttctag caattgtata tcgtagaaga gcatccgatt tctttatgga
      541 agtgattcga ttttcttcta ttaagctctg gattaggtat cagatttgga cctgttttct
      601 ttatggactc attttaatca tggtaaaagt tctagtgatt caatttatgt tacagttacc
      661 aaactgggat ataggagttt tgtttatagt tgattctttg aatgcttgtg tgttagtctt
      721 gttttgcttt atgttatacg cactaggagc gaatgtacaa atgaactttg cttgcgttag
      781 tttcttttta ctcatgattg tgtttggtgg tctatttgta ggaaatcgaa ctaattatct
      841 attttatatt ttgaatcgag ggaatggaga tataggacga gatttatttc ttcagttgct
      901 atttttagtc tttctttttc aaagcatttt ctatttcact cgtcaaaaga ggaggtttat
      961 agaatgaatg aaataattat attaaaaaat attgagttga aattaaaaaa aacatgtgtt
     1021 tttcaaaacc ttaactttag ttgtaaacag ggggaaatta taggaattac tggtgcgaat
     1081 ggctcaggga aaagtgtatt gtttaaatta atagctggtt tatatagtcc gtcttatgga
     1141 gaagtgttaa tcaatgggga aaatattgtt cctgagagaa aaattccagc taatttggga
     1201 gctttgattg aagaacctgg ttttataaat tattatagtg gctttaagaa tttacaatat
     1261 ttggcaagca tacgaggagt agttggtaat caggaaatca atgatacact gaaaatagtt
     1321 ggtctatatg agcaaaaaga ccagaaagtt aaaacttatt cgctaggtat gaggaaaaag
     1381 ctagggattg ctcaagcaat tatggagaat ccctctattc ttttactaga tgaacctatg
     1441 aatgccttgg ataaatcaag tgtagaaaat atgagaacat tgtttagaaa gctctctagt
     1501 gaaaaaggga caacaatttt gattgctagt catagtgaag aggatattcg tatcttatgt
     1561 gataaagtat atgcaataga agataaagta tgtacactgt gttcagattg aacatcttag
     1621 atagactcag gatgttatag gtaaaatctt aaaaagggat tggagcttaa aatccagttc
     1681 cttttaagag ttaatgacta atcaactttt ccaccttcaa caaccaggtc atctgcttta
     1741 gcagcgtcac cgtaggttgg gtgaagtgtt ttttcatagg ccttgtggaa gaagttttcc
     1801 ttgcctaatt tttcaatat
//
LOCUS       DAVKJD010000134         1790 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKJD010000134 DAVKJD010000000
VERSION     DAVKJD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.134"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            365..841
                     /locus_tag="VW157_001959"
     CDS             365..841
                     /locus_tag="VW157_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9553205.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            841..1602
                     /locus_tag="VW157_001960"
     CDS             841..1602
                     /locus_tag="VW157_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU9553206.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGEMVESLV
                     TKLSSLRYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT          654 a          244 c          348 g          544 t
ORIGIN      
        1 agtaaaaaca acactttatt gaggtattgg atacgctttg ctaatagcct aataatcaca
       61 tgtggagtgt tgctacaacg aaaaaggtga taatccttga tttcaagcta ttttataagc
      121 attttgtctt tgtagataaa ggcaattttg acaataaaaa tcctaaaagg tgaatcgtta
      181 tagatgtatt tgtagatatc gtttgcgcat cgaaaaaatt aatacaagaa taaatattta
      241 tggctcttta ggtgactttt atagaagtaa agtttaggat agaaaaacaa gaaataacgc
      301 accatttttg gtgcgttatg cttttttatg ctataatgga tttataaaaa taaaggagtt
      361 tgctatgatt ggaaagaaca taaaatcctt acgtaaaaca catgacttaa cacaacccga
      421 atttgcacga attataggaa tttctcgaaa tagcttgagt cgttatgaaa atggaactag
      481 ttcagtctct acggaattaa ttgacatcat ttgtcagaag tttaatgtat cttatgtcga
      541 tattgtagga gaagataaaa tgctcaatcc tgttgaagat tatgaattga ctttgaaaat
      601 tgaaattgtg aaagaaagag gtgctaatct attatctcga ctctatcgct atcaagatag
      661 tcagggaatt agcattgatg atgaatctaa tccttggatt ttaatgagtg atgatctttc
      721 tgatttgatt catacgaata tctatttagt agaaactttt gatgaaatag agagatatag
      781 tggctatttg gatggaattg aacgtatgtt agagatatct gaaaagcgga tggtagctta
      841 atggaaatcc aagattatac tgatagtgaa ttcaaacatg ctttagcaag gaatcttcgt
      901 tcactgacaa gaggaaaaaa gtccagtaag caacctatag cgattttgct tggaggtcaa
      961 agtggtgccg gtaagactac aattcatcgt attaaacaga aagaatttca aggaaatatt
     1021 gttatcatag atggtgatag ttttcgttct cagcatccac actatttaga actgcagcaa
     1081 gaatatggca aagacagtgt agaatatacc aaagattttg caggagaaat ggtagagtct
     1141 ttagtaacaa aattgagtag tttgagatac aatcttttga tagagggaac tttacgaact
     1201 attgatgttc caaagaaaac agcacaacta ttaaaaaata aggggtatga ggtacaatta
     1261 gccttaattg caaccaagcc cgaattgtcc tatctgagca cccttatccg atacgaagaa
     1321 ctgtatgcta tcaatccaaa tcaagcacgc gcaactccaa aagaacatca tgatttcatt
     1381 gtaaatcatc tagttgataa tacacgacaa ttggaagaac tggctatctt tgaaagcatt
     1441 caaatttacc aacgagatag aagttgtgta tatgattcaa aagaaaatac aacttcagca
     1501 gcagatgttc ttcaagagtt actctttggg gagtggagtc aggtagagaa ggagatgttg
     1561 caggtggggg aaaagagact taatgaatta cttgaaaaat aaacaattga tatttttagg
     1621 agaatagaaa tgagagggtt taataacaag ataaagtctg tttatcaaga actaacaaat
     1681 tccaaagaga aattcggtag ctttcacaag actttaattc atttgcatac acctgtttct
     1741 tatgattaca agctattttc taattggact gcaacgaaat atagaaaaat
//
LOCUS       DAVKJD010000135         1665 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKJD010000135 DAVKJD010000000
VERSION     DAVKJD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.135"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..273
                     /locus_tag="VW157_001961"
     CDS             <1..273
                     /locus_tag="VW157_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553207.1"
                     /translation="YLLYKVGFLGIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQD
                     AVASKQIVVINEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSSIKG"
     gene            588..1565
                     /locus_tag="VW157_001962"
     CDS             588..1565
                     /locus_tag="VW157_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553208.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKRDCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
BASE COUNT          562 a          222 c          280 g          601 t
ORIGIN      
        1 tatttactat ataaggttgg atttttggga atcatttacg ataatgaagc attaagagat
       61 ttgtcaaaac aatatttaaa caaaaatatt tcggaattac aagatggttc tttcgagggc
      121 ggtatacaag atgctgttgc ctctaaacaa atagtagtta taaatgaatt tatagcttgt
      181 ttaaattcca gatgcgtgcc atcccaatat gatagatttt tttataaaga tagaccttat
      241 attttttcca gaaagagttc tattaagggg tgaaaatttg ttatatttgg attaacaagt
      301 gtcctaggga ttagaaataa ctctagtata gctagatatt tttgagtaac tagaaatatc
      361 attaggctac ccatatgatt gccagaaaag atggctgatg aatcttattt atactgttgc
      421 agtaggcatt gtctaggata aataagctat cttgatgggg gaaattggaa taattttagt
      481 agaaacatta aaggattatg gacaagtagc gcaggattag gtgatgcaat gtgctaagtt
      541 ggcttggtgt tagcagtagt attatttaac aatggaaagt agactaaatg aaaaataagc
      601 gttatttttt tgacacaatt ttaatgattt tactgcttat aagcacgata ttttgtgtca
      661 gcccagtatt tataaaattg gacatcttag gaaccccctc tcatgccatc ttaacctttg
      721 tattggcgat accgttattc tatattttgt ctcaatgctt acatactttg ctattactag
      781 tttcctcaat cttttgtaaa ttaagaccaa tttattttta ttttatattt gtgatcatta
      841 taggagctag gaaatactat cgaattctct ttcaccaact aatgggattt agtccaggaa
      901 tagcagtgtt ttataaggag agtcaaacaa ctaagaatct gtttaaattt tactacttct
      961 tgtactttac aacactaatt tcatactatt ttttctttac ttttgtgtat gacaaaccgt
     1021 tgcttttgcc tcttattcca ttttctatta tcatagcatt agtacaaaaa ctttatagaa
     1081 ttgagaatca acaattattt ctgttaaaat caaaggtact aacaatactg gaatctaaga
     1141 gggattgtga gtttaatctt caggattatc atgaaatttg gaaattgcaa tcaaaatctg
     1201 agttgccatg cgtggcttta tcttatatca gtttgatcaa gccatatctt tcagagagcg
     1261 tgcgtgaaca aattgattta ttagaagtta agcgatttaa aaaaataaat catccaatat
     1321 cactttatgg tatgttggat gttataaaat taaatctata tttacggcat tataatgaaa
     1381 aaaataaata tgaatcaatg ttaaagaaaa ttttagaggt aagaccagac tttgttttaa
     1441 tcgagcagaa tattgatgat agtttaaata gctcgcaacc attatcttta tctttagcca
     1501 tttcagaaat ccaacttttg cttgaagtgt atatggggat caaacatgtt tcaattagga
     1561 gataaaacta gaataacatt tgctattata ggggcaagtt gcatattagt cagtatgtat
     1621 cttatacctc tcattaacac ggataataaa ttttgggaaa tactt
//
LOCUS       DAVKJD010000136         1619 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKJD010000136 DAVKJD010000000
VERSION     DAVKJD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.136"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..346
                     /locus_tag="VW157_001963"
     CDS             <1..346
                     /locus_tag="VW157_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9553209.1"
                     /translation="KFNVSGNKGQKNNIELIAKQRSFIDYWAKYVSYLPFPTPEFFLA
                     NLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITSEDIFQEQRRAVSKIQPESSM
                     FQCIKEKLEALF"
     gene            726..1529
                     /locus_tag="VW157_001964"
     CDS             726..1529
                     /locus_tag="VW157_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9553210.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT          533 a          258 c          304 g          524 t
ORIGIN      
        1 taaatttaat gtttcaggta ataaagggca aaaaaataat attgaattaa ttgcgaaaca
       61 aagaagcttt atagattatt gggctaaata cgtttcttat cttcctttcc caaccccaga
      121 gtttttttta gctaatttgt gtaacagcgt agatagggaa ggttatgatt tttctaaaga
      181 tggcaacgga aaagaatatt ttagaaaaaa aactcaagta gcgttaggaa tagagaatat
      241 aacttctgaa gatattttcc aggagcaaag aagagctgtt tcaaaaatac agccagaatc
      301 atcaatgttt cagtgtatta aagaaaaatt agaggcattg ttctaattcc ttagattagg
      361 tactacataa caaatttttt aaattcaaac ccgcaccaaa aacaacattg caactttctt
      421 ctcaggtaat gtgttgggat ttgaacagac caacgagttc atagtggtgt atgccaatga
      481 ctttgataag tctacgcgtc agacttatta gttcatttat ccagatatat ctcgataatc
      541 atattattta gtcaaagaac atcccatatg agggcatgaa atgctaagtt tagcttgctc
      601 actattaaga tgaagtatat atatttcata gaacctattg tgaggacttt ttatagttta
      661 agccagtttc caaccaattt cgtgatttac tggtaatgaa gaaaaacaat tgaaaggata
      721 tttctatgca atcaacttac aacattgaca atccaaactt gtcttatgaa gcgaagcgtg
      781 atttatggcg gataggtttt ggtctgcaga aagttgacaa tctagtgcca tcagcgtata
      841 tggaatcttt ggctgagaaa cagtcccggg gagaactgac ttatgagcag gtttatgagg
      901 atgcaacggc ttatcaccat actattgatg caagtacgga agaggcagac ttggtttctc
      961 tacgtattgt agaactattg tctcgaagag gctttagctt tagtcctgcg accttacttg
     1021 ctattcataa ggagttgttt caagatatat ttgaaccctc tattccggtg gggcaatttc
     1081 gtcagaccaa tatcacaaag aatgaacctg ttttgaatgg tgaaagtgtt gtgtactctg
     1141 attactccat gattcaaatg accttagatt atgattttaa tcaggaaaaa caagttgcat
     1201 atgcgacact aactcaggcg gatgtggtta aacaaatcca gcattttatt tcaggaatct
     1261 ggcagattca tccatttcgc gaaggaaaca ctcggacagt aacggtcttt ttgattcagt
     1321 atcttcgtga gtttggtttt gatattgata atacaccatt tcagcaacat tccaaatatt
     1381 ttcgtgatgc cttggtatta gataatgcaa agattttaca gcgacgttct gagtttttaa
     1441 cagctttttt tgaaaatctc ttgctcggtg gtcaaaatga tttgtcttca gaaaaaatgt
     1501 atctagattt agacctcgat ttttcataat ccaaatactg agtaaacatt gaattttagg
     1561 caaaaatgaa gtaaatattc tcacaagaaa acgtatatca tcaaagtttt aggtgtact
//
LOCUS       DAVKJD010000137         1602 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKJD010000137 DAVKJD010000000
VERSION     DAVKJD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.137"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(294..851)
                     /gene="sdbB"
                     /locus_tag="VW157_001965"
     CDS             complement(294..851)
                     /gene="sdbB"
                     /locus_tag="VW157_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9553211.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIS
                     VGKDVPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            complement(862..1569)
                     /gene="ccdA2"
                     /locus_tag="VW157_001966"
     CDS             complement(862..1569)
                     /gene="ccdA2"
                     /locus_tag="VW157_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9553212.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQERAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
BASE COUNT          539 a          329 c          260 g          474 t
ORIGIN      
        1 aaaaaatctg actaccgtaa cttctcaaag taacttccat ctctctccca aaactggaag
       61 ttactctaag acgactctgt tttagggcta tttgtcgtgc actttttctg agtcttttcg
      121 cttttgaacc cttatatatc aagaaaaaga aaaccagtgg aagttagtct aagacggatt
      181 tccactgatg tcacgcgttt ttctcatact aaaagctagg ttgtgggaaa ctggaagtta
      241 gttttagaag ttaccaaaat ctgactacat cttgtaatca gattttatcg attctagttc
      301 atttctttaa atgctgcttc tgcatccgca ttactgatag caccaaattg aatctttcca
      361 atctttcctt ggctatcaat taaatattct gtaggaatgc ttcgaatttg ataagcttgg
      421 aaggtggttg ctttggtatc ataaagaact gggatatcct tatatccttg ttcttggaac
      481 cattgtggga attgctcaac agttttttca ccttgaattc ctggtgcaat gacagtaaga
      541 atttcgaaat cacgatctgg tttcgccgct agttccatca actctggcat acttttcttg
      601 catggaccac accatgaagc ccaaaacttc aagtaaacct ttttaccctt aaaatcagat
      661 aacttaactt ctttgccatc catggattgc aatgtaaagt ctggaacatc ttttccaaca
      721 gaaatttgtt gtacagtcgt ttgttgtttt ggctgttgtg ctgcttgagt ctttttagtt
      781 tcttcctcac cacaggccat caatacaact aatgacaaga gacttaagcc agcaaacatt
      841 acttttttca tttgtcctcc tttattcaaa aattccagct agaacattta cttgtcctaa
      901 tagtaacaaa attcccatta aaacaatgag gaaaccacca attttcttta gtagcatcat
      961 atgacgcttg attttactaa aatatggcat gactagacct gaagctagtg ccaagaccaa
     1021 gaaaggaagg gccatgccca gagtgtaaat gagagtataa atcgctcctt gccaagcacc
     1081 attgcctcca gaagccgcaa gtgctaaaac agaacttaaa actggaccaa tacaaggtgt
     1141 ccaaccaaag ctaaaggtaa taccaagtaa aaaagctgac caataacgat tagaatctga
     1201 ttttttaaag gtaaaacttt tttgaacttc taatttcttc aaatgaaaaa tttccatctg
     1261 gtgaagaccc aaaatgataa taatagctcc catgacatat cgaaaccaat ttgcatagag
     1321 aatatgacca aagtaaccag caccaaagcc tagaataaag aaaatgagag agataccagc
     1381 gataaagcaa agtgttcgaa tcaagcctga ccagagaacc tttctcccaa acaaagaaaa
     1441 gctttttgcc ctttcttgat catccaataa aatcccagca tagactggca gaagaggaaa
     1501 aatacaagga gaaaaaaagg ataaaacacc tgctagaaaa acagagatta aaaatactat
     1561 cgtttccaat aaagaaccaa ctttcttaat aattctaatc ct
//
LOCUS       DAVKJD010000138         1550 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKJD010000138 DAVKJD010000000
VERSION     DAVKJD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.138"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            295..1167
                     /locus_tag="VW157_001967"
     CDS             295..1167
                     /locus_tag="VW157_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9553213.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(1313..1550)
                     /locus_tag="VW157_001968"
                     /pseudo
     CDS             complement(1313..1550)
                     /locus_tag="VW157_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          509 a          273 c          305 g          463 t
ORIGIN      
        1 tatgtttttc acctctaaca atcgcaatct cttctttatc catgaatgaa atcgctttct
       61 atttttgtaa gtaaagcata acacgaaatc cacgaaaatg aaaacctttg ttgtgttttc
      121 gtaaaaaatt tgttgacaga gcacgaaacg cctactataa ttaaatcaag cttaaggaaa
      181 taacaaaaaa acaaatcgga gggaaagacg atgaacacat taaatgagaa agtaatcaat
      241 atctgtaaag caatagttaa agaaacttta atccaagaca tttaggagaa agaaatgaag
      301 aaatcaagaa aattagccac attaggaatc tgttcagcct tgtttttagg cttggctgct
      361 tgtcaacaac aacatgctac ttctgagggg acgaatcaaa ggcaaagcag ttcagcgaaa
      421 gttccatgga aagcttcata caccaaccta aacaaccagg taagtacaga agaggtcaaa
      481 tctctcttat cagctcactt ggatccaaat agtgttgatg cattttttaa tctcgttaat
      541 gactataata ccattgtcgg ctcaactggc ttatcaggag atttcacttc ctttactcac
      601 accgaatacg atgttgagaa aatcagtcat ctctggaatc aaaagaaggg cgatttcgtt
      661 gggaccaact gccgtatcaa tagttattgt cttttgaaaa attcagtcac cattccaaag
      721 cttgaaaaga atgaccaatt gcttttccta gataatgatg cgattgataa aggaaaggtc
      781 tttgattcac aagataagga agagtttgat attctatttt cgagagttcc aactgaggca
      841 actacagatg tcaaggttca cgctgaaaag atggaaacat tcttctcaca atttcaattc
      901 aatgaaaaag ctcgaatgct gtctgtagtc ttgcacgaca atttggatgg cgagtatctg
      961 tttgtaggcc acgttggggt cttagtacct gctgatgacg gtttcttatt tgtagagaaa
     1021 ttgactttcg aagagcccta ccaagcgatt aaatttgcta gtaaggaaga ttgctacaag
     1081 tatttgggca ccaagtatgc ggattataca ggcgagggac tggctaagcc ttttatcatg
     1141 gataatgata agtgggttaa actttaatag tgttatgata tgacagtaat cttaggattt
     1201 ttatttctat ttttgatgag ttggtgggtt atttcaatcg ttaatagcca gaaaccacgc
     1261 aaaaatgaaa catttattgg ctatatccaa agatacgata ttgatgggaa tggctatcaa
     1321 tcattaccgt gtcctcagaa ctgagaggag ttcttgaaac cgtaaaaccg ctttgaacaa
     1381 gagttacttc aacccattgg ctccgacgga gtaagttgct ttcgtgaata ccaaaatcag
     1441 ccgcaatttc ttcataagtt cgatattctc gcacatattg aagagtggcc ataagaaggt
     1501 cttctaggct taatttaggt tttcgtccac cttttgcgtg tttaagttga
//
LOCUS       DAVKJD010000139         1523 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKJD010000139 DAVKJD010000000
VERSION     DAVKJD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.139"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            27..1373
                     /gene="gdhA"
                     /locus_tag="VW157_001969"
     CDS             27..1373
                     /gene="gdhA"
                     /locus_tag="VW157_001969"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9553214.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHGGSIWTYAGNYDIALPCATQNEINGEAAKR
                     LVDQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          460 a          292 c          327 g          444 t
ORIGIN      
        1 aaatatgaca ataaaaggag aacgatatga catctgctaa agaatatatc caaagcgtgt
       61 ttgaaactgt gaaagctcgt aacgggcacg aggctgaatt cctccaagct gttgaagaat
      121 ttttcaacac tttagaacct gtatttgaaa aacaccctga gtatatcgaa gaaaatatct
      181 tggcacgtat tactgagcct gagcgcgtgg tttctttccg tgttccttgg gttgaccgtg
      241 atggaaaaat tcaagtaaac cgtggttacc gtgttcaatt caactcagct gttggaccat
      301 acaaaggtgg acttcgtttc cacccaactg taaaccaagg gatcttgaaa ttcctcggat
      361 ttgaacaaat ctttaaaaat gtcttgactg gacttcctat cggtggaggt aaaggtggat
      421 cagacttcga tcctaaaggt aaaacagatg ctgaagtgat gcgcttctgc caaagcttca
      481 tgactgaatt gcaaaaacac atcggaccat cacttgacgt acctgctggt gatatcggtg
      541 ttggtggacg tgaaattggt tacctttacg gtcaatacaa acgtcttaac caatttgatg
      601 ctggtgtctt gactggtaaa cctcttggat ttggtggtag cttgattcgt ccagaagcaa
      661 ctggttacgg tttggtttac tatactgaag aaatgctcaa agctaacggt aacagctttg
      721 ctggtaagaa agtcgttatt tcaggttctg gtaacgttgc tcaatatgct cttcaaaaag
      781 caactgaact cggtgcaact gttatttctg tttctgactc aaatggttat gtcatcgatg
      841 aaaatggtat cgacttcgat cttttggttg atgttaaaga aaaacgtcgt gctcgtttga
      901 ctgagtatgc agctgaaaaa gcaactgcta cttatcatgg aggttctata tggacttacg
      961 ctggtaacta tgatatcgct cttccatgtg cgactcaaaa cgaaatcaac ggtgaagcag
     1021 ctaaacgttt ggttgatcaa ggcgttatct gtgtatctga aggtgccaac atgccaagcg
     1081 accttgatgc catcaaagtt tacaaagaaa atggtatctt ctacggacct gccaaagctg
     1141 ccaacgctgg tggtgtagcc gtttcagccc ttgaaatgag ccaaaatagt cttcgcctct
     1201 catggactcg tgaagaagtt gatggccgtc tcaaagacat catgacaaac atcttcaaca
     1261 cagctaaaac gacttcagaa acatatggtc ttgataaaga ctaccttgca ggagcaaaca
     1321 ttgctgcctt tgaaaatgta gcaaacgcta tgattgccca aggtattgtt taagatttat
     1381 ttgcttaatc ctcaatcact acgattgggg atttttagtt gagttagaca gttgggaaat
     1441 tctaattaaa tgaaataaaa actgaacaaa ttgattgcgg aattcaaacc aatttctaac
     1501 atatttctag aaattaattt gac
//
LOCUS       DAVKJD010000140         1520 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKJD010000140 DAVKJD010000000
VERSION     DAVKJD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.140"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            37..1428
                     /locus_tag="VW157_001970"
     CDS             37..1428
                     /locus_tag="VW157_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9553215.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
BASE COUNT          382 a          277 c          325 g          536 t
ORIGIN      
        1 gtaaaataga attatcagtt tattttggag tcaaagatga atatatttag aacaaagaat
       61 gttagtttag ataaaacaga gatgcatagg catttgaagt tatgggattt gattttgctg
      121 ggtatcggag ccatggtagg gacaggcgtc tttacaatca caggtactgc agctgcaaca
      181 cttgctggcc cagccctagt gatttcaatc gttatttctg ccttgtgtgt gggattatca
      241 gccctctttt ttgcagaatt tgcctcgcga gtacccgcta caggaggtgc ctatagttac
      301 ctctatgcta tcttaggaga attccctgcc tggttggctg gttggttaac catgatggag
      361 ttcatgacag ccatatcagg cgtagcttcg ggttgggcag cttattttaa aggcttgctc
      421 tctcaatacg ggatagcctt tcctcaggct ttaaatggta cctttaatcc tcaagcaggg
      481 acatttgttg atttattgcc tattcttgtc ttggtcttgg tgacttcgct tgttttacta
      541 aatgccaagg cagccttacg ctttaattcg attctagtta ttttgaaatt ttccgcttta
      601 gctctctttg ttttagtagg aatttggaat attaagtttg ataattggag caattttgct
      661 ccttacggtt ttggacaaat ctatggagct agtactggta ttatggctgg tgcatccttg
      721 atgttcttcg gttttttggg atttgaatcc atctctatgg ctgtagatga agtcaagact
      781 cctcaaaaaa atattcctag agggattgtc ttatcgcttt ctatcgtaac cattctctat
      841 gccttggtga cccttgtttt gactggtgtg gttcactata gtcatttaaa tgtcgatgat
      901 gccgttgcct ttgcccttcg tagtgttggg attagttggg cagccaacta tgtgtcatta
      961 gtggctatct tgaccttgat tacagtttgt atttcgatga cctatgccct atcgcgtatg
     1021 atttacagtt tagcacgtga cggcttagtg cctgctgcct ttaaggaact gacgaagact
     1081 agcaagatac caaagaatgc tactatcttg acaggtctag cttcagcagt agcagcagga
     1141 atgttcccac tagccagtat tgcagccttt ttaaatattt gtaccttagc ctacttgatc
     1201 atgttggctt atggtctgat tcgcttacgg aaagaaaaag gaatgcccaa agctggggaa
     1261 tttaaaacac cattggtacc tctgttacca attttatcga tcatcatttg tttatccttc
     1321 atgttacagt ataatatgaa tacctggatt gctttcctag ttgcgttgtt agtaggaagt
     1381 attatttact tcacttatgg ctataagcat tctaccatag aagaataaag tgaaagtcta
     1441 ttgagttgtt gacactcagc agatttttat atgtgaaaga aatttcaaat tttttctaaa
     1501 aatatcttga cagattaaat
//
LOCUS       DAVKJD010000141         1464 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVKJD010000141 DAVKJD010000000
VERSION     DAVKJD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.141"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            78..>1464
                     /gene="pcpA"
                     /locus_tag="VW157_001971"
     CDS             78..>1464
                     /gene="pcpA"
                     /locus_tag="VW157_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU9553216.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDIEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNRGV"
BASE COUNT          579 a          201 c          230 g          454 t
ORIGIN      
        1 ataaaacttt ttataacaac ggctagcaaa gttataattt tatatctatt agaaaataaa
       61 tatgtaagga aatttaaatg aaaaaaacta caatattatc attaactaca gctgcggtta
      121 ttttagcagc atatgtccct aatgaaccaa tcctagcaga tactcctagt tcggaagtaa
      181 tcaaagagac taaagttgga agtattattc aacaaaataa tatcaaatat aaggttctaa
      241 ctgtagaagg taacatagga actgttcaag tgggtaatgg agttactcct gtagagtttg
      301 aagctggtca agatggaaaa ccattcacga ttcctacaaa aatcacagta ggtgataaag
      361 tatttaccgt tactgaagta gctagtcaag cttttagtta ttatccagat gaaacaggta
      421 gaattgtcta ctatcctagc tctattacta tcccatcaag cataaaaaaa atacaaaaaa
      481 aaggcttcca tggaagtaaa gctaaaacta ttatttttga caaaggcagt cagctggaga
      541 aaattgaaga tagagctttt gatttttctg aattagaaga gattgaattg cctgcatctc
      601 tagaatatat tggaacaagt gcattttctt ttagtcaaaa attgaaaaag ctaacctttt
      661 cctcaagttc aaaattagaa ttaatatcac atgaggcttt tgctaattta tcaaatttag
      721 agaaactaac attaccaaaa tcggttaaaa cattaggaag taatctattt agactcacta
      781 ctagcttaaa acatgttgat attgaagaag gaaatgaatc gtttgcctca gttgatggtg
      841 ttttgttttc aaaagataaa actcaattaa tttattatcc aagtcaaaaa aatgacgaaa
      901 gttataaaac gcctaaagag acaaaagaac ttgcatcata ttcgtttaat aaaaattctt
      961 acttgaaaaa actcgaattg aatgaaggtt tagaaaaaat cggtactttt gcatttgcgg
     1021 atgcgattaa acttgaagaa attagcttac caaatagttt agaaactatt gaacgtttag
     1081 ccttttacgg taatttagaa ttaaaagaac ttatattacc agataatgtt aaaaattttg
     1141 gtaaacacgt tatgaacggt ttaccaaaat taaaaagttt aacaattggt aataatatca
     1201 actcattgcc gtccttcttc ctaagtggcg tcttagattc attaaaggaa attcatatta
     1261 agaataaaag tacagagttt tctgtgaaaa aagatacatt tgcaattcct gaaactgtta
     1321 agttctatgt aacatcagaa catataaaag atgttcttaa atcaaattta tctactagta
     1381 atgatatcat tgttgaaaaa gtagataata taaaacaaga aactgatgta gctaaaccta
     1441 aaaagaattc taatcgggga gtag
//
LOCUS       DAVKJD010000142         1426 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVKJD010000142 DAVKJD010000000
VERSION     DAVKJD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.142"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(114..389)
                     /locus_tag="VW157_001972"
     CDS             complement(114..389)
                     /locus_tag="VW157_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9553217.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(495..1334)
                     /locus_tag="VW157_001973"
     CDS             complement(495..1334)
                     /locus_tag="VW157_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9553218.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGHILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRAVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
BASE COUNT          437 a          298 c          218 g          473 t
ORIGIN      
        1 acaatatttt cggaacattc aactttttaa ctctatttat tactagattt cataattaaa
       61 aagcctactg accaagctag aaagcttgat acaataggct ttttaaagac tgattattta
      121 acagcgtctt taagagcttt accagctttg aatgctggta ctttagaagc tgcaattgtc
      181 atttctttac cagtttgtgg gttgcgacct ttacgttctg cacgctcacg aacttcaaag
      241 ttaccaaaac cgatcaattg aactttttca ccagctgcaa gatagtcagc tactgctgca
      301 aatacagctt caactgctgc tgctgagtct ttcttagtca attctgtagc ttctgctact
      361 ttagcgatca aatcttgttt gtttgccatg ttaatgattc ctccagataa tttctaatta
      421 acaaatataa tcatatccta aaatcccttt taggtcaagt taaaaacgct atttcttcca
      481 attttattta ttttttagga gtggtaacgt atcaaaatag cccaagcgtt ctcacccgtg
      541 tgagtttgaa taatggaacc cgtttccaaa acagaaattg gcttttcaac ataagcttgt
      601 aagctttctt tcatctcttt tgcccaatca tcactaccag aatatgaaat tccaatctct
      661 gctacagcac gttcagaaag cgatgttatc aactcatcta accatttttt aaatgtttta
      721 gttccacgac ctttaaccat tggctgcaat tcatggtctt tcatttgcat gacagcacgg
      781 atattgagaa gagagctcaa caagccagtt acacggctaa ttcgtccacc tttgacaaga
      841 ttttccaaag ttgaaacacc aatataaagc tctgtatggt ttttaacctc ttctacatga
      901 gataaaattg cctccatatc tttaccttct tgagctaact tcgcagcctc aacaacttgg
      961 aatttcaggg cttggtcagt gaaggaacta tcaacaacag tcacatctgc agtagatagg
     1021 ctagcacctt ggcgtgctgc ttctaccgta cccgaaagag catgggacat atgaatagca
     1081 agaatatggc caccatcttt gcataggtct tcaaaaatct cagcaaagac acctacaggt
     1141 ggctgacttg ttttcggaag attcttactt tcttgcatca actgaagaaa tttaccttct
     1201 tctttcaaat ccgcatcaga ataaacaaca ttatcaatca ttacagataa tggaacaatt
     1261 gtaatatcta attgctttac tagttcaggt tcaatagtaa cagatgaatc ggttacaatc
     1321 ttaatttttg tcatagtatc aatctttcta ttttaggatt cagattggtt tccttacttc
     1381 taattatatc aaaaaaaaga ttaaaaatcc taatggagtc aatcaa
//
LOCUS       DAVKJD010000143         1420 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVKJD010000143 DAVKJD010000000
VERSION     DAVKJD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.143"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            1..421
                     /locus_tag="VW157_001974"
                     /pseudo
     CDS             1..421
                     /locus_tag="VW157_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            588..788
                     /locus_tag="VW157_001975"
     CDS             588..788
                     /locus_tag="VW157_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553219.1"
                     /translation="MVVSASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            813..1230
                     /locus_tag="VW157_001976"
                     /pseudo
     CDS             813..1230
                     /locus_tag="VW157_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          487 a          288 c          293 g          352 t
ORIGIN      
        1 gcttatttga ctgaaatagc ttctgaattt ggctgtcatc caactaccat ccactatgcg
       61 ctcaaagcta tgggctacac tcgaaaaaaa gaaccacacc tactatgaac aaaacccaga
      121 aaaagtagcc ttatttctta agaattttaa tagtttaaag cacctagcac ctgtttagat
      181 tgatgaaaca ggatttgata cttattttta tcgagaatat ggtcgctcat taaaaggtca
      241 gttaagaaga ggcaaagtat ctggaagaag atatcagagg atttctttgg ttgcaggtct
      301 aacaaatggt gaattaatcg ctccaatgac ttacgaagag acgatgacga gcgacttttt
      361 tgaagtatgg tttcagaaat ttctcttacc aacattaacc acaccatcgg ttattatagt
      421 aaaatggaat aagaacagga caaatcgatc aggacaatca aatcaatttc tagcaatgtt
      481 ttagaagtcc atatgtacta ttctagtttc aatataccat aactgctcct agtcaacctg
      541 caagtcctgt cccagctcca agctcagctc cgactcaaga tcctagagtg gtagtaagcg
      601 cttcggctag ctcaacgagc acacaagctc aagagcaagt tgacaagtct gaacttcgtg
      661 ccttgagtca agagttagac caacgcttga aagctttggc aacagtatct gatccgaaaa
      721 tcgatgcaac caaggctgtc ctcctagatg ctcaaaaagc tccggaagat agtgccttga
      781 cagagtaagg tctgcatact gcagtagagt ctgtgaagac tgccttggac tctctcaaag
      841 atgtgatagc aaatgctagc gacagcaagc ctacacagga caagaaggat gcaaaacaag
      901 gaacggaaga tagtaaggat tcagataaga tgactgaaac aaactcagtt ccagcaggag
      961 tgattgtggt cagtctactt gccctcctag gcgtgattgc cttctggcta attcgccgta
     1021 agaaagagtc agaaatccag caattaagca cggaattgac caaggttcta ggacagctag
     1081 atgcagaaaa aagcggataa aaaagtcctt gccaaagccc aaaaccttct ccaagaaacc
     1141 cttgatttcg tgaaagaaga aaatggctca gcagagacag aaactaaact agtagaggag
     1201 cttaaagcaa tccttgacaa actcaagtaa aacatgattt atgaaatgtc agacctaaaa
     1261 gacattttcg ttcacaaaag cagcgacgag taatccttgt cgttgctttt tggctatttt
     1321 gggggataag atccgccttg actagcaaaa gaaaagattg gtccaatagt gcccgatacg
     1381 attttgcgta gcagaaagct tacaaataaa attgaatata
//
LOCUS       DAVKJD010000144         1365 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVKJD010000144 DAVKJD010000000
VERSION     DAVKJD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.144"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..>1365
                     /locus_tag="VW157_001977"
     CDS             <1..>1365
                     /locus_tag="VW157_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9553220.1"
                     /translation="FVPDKTNNEWFKEQTKAYIVEEKSTIKEVSDKQSIAGSPYSIGV
                     YDRLTSPSWKYPSMVLPLLTLPEKSVFIIANISTIGFGAYDRYRSKEHPAGTDLNDYV
                     EKKAKEAAVRFRDHYDYWYRILDDKNKEKLYRSVLVYDAFRFGTDEKEDKDTYQATFE
                     TNHPAIKHFFGPAGNNVVHNSNGAYATGDAFYYMAYRMLDKDGAVTYTHEMTHNSDRE
                     IYLGGYGRRNGLGPEFYAKGLLQAPDHPNDPTVTINSILKYDQSEESTRLQVADPTQR
                     FGSVDDLNKYMHNMFDVIYMLEILEGKAVAKLDTNQKYDLLRKIENEYKPDPDGNSVY
                     ATNVVRRLKPEELTKLTTFNSLIEHDIITRRGYVDEATYKRNGYYTINLFSPIYSALS
                     SKIGTPGDLMGRRIAFELLAAKGYKDGMVPYISNQYEKEAKAQGKVITSYGKQIGLVT
                     DEIV"
BASE COUNT          529 a          185 c          256 g          395 t
ORIGIN      
        1 catttgtacc agataaaaca aataatgagt ggtttaaaga acaaactaaa gcatatattg
       61 tagaagaaaa atctactatt aaagaggtaa gtgataagca atcaatagct ggtagtccat
      121 attcaattgg agtatatgat agattaacta gcccatcttg gaaatatcca agtatggtgt
      181 tgccgctatt aacattacct gaaaaatcgg tgtttattat agccaacatt tcgactatag
      241 gtttcggtgc ttatgataga tatagaagta aagaacatcc agcaggaact gatttaaatg
      301 actatgttga gaaaaaagct aaagaagcgg ctgttagatt tagagatcac tatgattatt
      361 ggtatagaat actagatgat aaaaataaag aaaaattata tagaagtgtt ctagtctatg
      421 atgcatttag atttggtaca gatgaaaaag aagataaaga tacatatcaa gcaacttttg
      481 aaactaatca tccagctata aaacatttct tcggtccagc gggaaataat gttgttcata
      541 attctaatgg agcatatgct acaggggatg cattctatta tatggcatat cgcatgcttg
      601 ataaagatgg tgcggtaact tatacacacg aaatgacaca taactcagat agagaaattt
      661 atcttggagg ctatggaaga cgaaatggac ttggaccaga gttttatgct aaaggactgc
      721 tacaagctcc tgatcatcct aatgatccaa cagtcactat taattccata ttaaaatatg
      781 atcaatcaga agaatcaaca aggcttcaag tagcagatcc aactcaacga tttggaagtg
      841 ttgatgatct aaataagtat atgcataata tgtttgatgt aatatacatg cttgaaatac
      901 tagaaggtaa agctgtagct aaattagata caaatcaaaa atatgattta ttaagaaaaa
      961 tagaaaatga atataaacct gatcctgatg gaaattctgt ttatgcaacg aatgttgtac
     1021 gtagattaaa accagaagaa cttactaaat taactacatt taacagtttg atagaacatg
     1081 atattataac aagaagaggt tatgtagatg aagcaactta taagcggaat ggctactata
     1141 caattaatct attctcacca atttattcag cattgagcag taaaatagga acaccaggag
     1201 atttaatggg acgacgtatt gcatttgagt tattggcagc caaggggtac aaagacggta
     1261 tggttccata tatttctaat cagtatgaaa aagaagcgaa agctcaagga aaagttatta
     1321 catcatatgg taaacaaata ggtttagtta ctgatgaaat tgttc
//
LOCUS       DAVKJD010000145         1358 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVKJD010000145 DAVKJD010000000
VERSION     DAVKJD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.145"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(294..476)
                     /locus_tag="VW157_001978"
     CDS             complement(294..476)
                     /locus_tag="VW157_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553221.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKADHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(478..1257)
                     /locus_tag="VW157_001979"
     CDS             complement(478..1257)
                     /locus_tag="VW157_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553222.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELDPKDIPTL
                     LEQGTTTLLSQEILDRVANACIRDNVLLASGTSVLAGLAGGLAMAITIPADVAQFYTF
                     SLKLAQELGYIYGYEDLWASREELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGIGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
BASE COUNT          445 a          293 c          203 g          417 t
ORIGIN      
        1 tatatactgc aaggtgaagc tgacgtggtt tgaagagatt ttcgaagagt ataaaatgat
       61 tttactgctt atattatacc atatggaaag agaataaaaa actagcagtg gagttcctgc
      121 tagttctctg caacttggat taccatctag ataagtttaa agagccaatc caatatctta
      181 aacactatct tgtaaaacag caattggact gcaaaagaga tgatcatcca aaagaatttc
      241 acaatcccaa taatcggttt gataaaaaga atagcaagaa gaccaaaaaa atttcattct
      301 ctctcctctg gcttaatgag ccaccgagcc gtatccatta acttgtcggc aataaaaagt
      361 ttccccagac caacaacgac atggtcagct ttctttatcg ttttcatcac ttttacacct
      421 tgcacggtca aaaagtaatt accataaatt ttagcaaaag ctttgatagg attcatatta
      481 ctctcctttg atgatttcag cctcttttcg gattgtttca acatcttctt gatattgaac
      541 ttcactatag ttgactagct tggataattc tttctgcaag ctttccccca ttggtttcat
      601 agttgcaaag gttaaaccac ctgaaatgat accacccaag ataggaataa atttccctat
      661 tcctttggcc agccctccct tggtaagatt cacaccaaat attcttaaga cttttttcaa
      721 aatagggtac caaagcgtct ttgttaaagc tttattaggc actgttttca ttacctgttt
      781 ggcaattgtt ataccaccag cacgtagcaa agcagcggtt ccattcaccc ccaacatgac
      841 gcctagataa agcaagaggg tattttgagc atcttcactc aactcctcgc gtgaagccca
      901 aagatcctca taaccataaa tataacctaa ttcttgagcc aattttagag agaaagtata
      961 aaattgagcc acatcagctg gaatggtaat tgccatagca agccctccag ctaatcctgc
     1021 caaaacagaa gtcccactcg ctaataatac attgtcccga atacaagcat tagctacacg
     1081 atctaatatt tcttgagata gaagagtcgt tgtaccttgt tccaataagg taggaatatc
     1141 ttttggatcc aattctttgg aaaacttatc cactaaaaat ttcgaacgat caaccttaac
     1201 aacaggtagt ttcaccactt gttgcaatac ttgcatagct aagtcttgtt cagccatata
     1261 caaactcctt atttcttttt tacaccttat catattatat ttcctacaaa aaaagcattt
     1321 tctaattaga taacattaaa aaatacagaa cccctttt
//
LOCUS       DAVKJD010000146         1298 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVKJD010000146 DAVKJD010000000
VERSION     DAVKJD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.146"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            246..869
                     /locus_tag="VW157_001980"
     CDS             246..869
                     /locus_tag="VW157_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9553223.1"
                     /translation="MTIDLLNVSKSFGSKKIFTDLNLIFESGKSYALIGGSGSGKSTL
                     LNIIGRLEKIDSGNVLVDKQDIWKIKERTFFKNTVGYVFQNYSLIDNKTVYDNLSLIT
                     KDKKTITDVLEKVGLSSDYLHQKIYELSGGQAQRVAIARMLMKPRKIILADEPTGALD
                     GEIGKEIIRLLLNETAEDKYVIIATHDPAVYNEVDVIIDMKDIGYKV"
     gene            866..>1298
                     /locus_tag="VW157_001981"
     CDS             866..>1298
                     /locus_tag="VW157_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553224.1"
                     /translation="MKKKIYIALIFVTGVLAIFFFGKQMITKENINKPTVELTIYTLS
                     SSDTEKWNKVRQVETEEAIYFITVKEVSSSEEVFSNIIANGAATGFGVREEEVKKFNN
                     GLGDTIEDSKHNKLIEIEFFTFSDDGAGFVVANFDYGKEELN"
BASE COUNT          488 a          162 c          250 g          398 t
ORIGIN      
        1 ctaaaacaga atgaatatgt gaaaaaaatc attttgggtc tgtcaaaaac atatatagca
       61 atttcaagtc tcaaatattt tatgatgact attacaatgg ttattctatt tacatttctt
      121 atgacagggc aaatagagtt gctatatatt ggagcggctt ctctgctagt tttgatgttg
      181 tcaatcataa tgagttttag aaaattatct gaaagctaca ctaaaatttt aaaaggagat
      241 gaatcatgac aattgacctt ttgaacgttt caaaaagttt cggctcaaag aagatcttta
      301 cagacttaaa tttaatattt gaatctggaa aaagctatgc attgattgga ggttctggct
      361 ccggtaaaag tacccttctt aatattatag gaagattaga aaaaattgac agtgggaatg
      421 ttttagttga taaacaagat atttggaaga taaaagaaag aacttttttt aaaaatactg
      481 ttggatatgt atttcagaat tattctttaa tagataacaa aacagtgtat gataatctga
      541 gccttatcac taaagacaaa aaaacaataa ctgacgtact tgaaaaagta ggactgtcaa
      601 gtgactattt acaccaaaaa atatatgaat tgtctggagg gcaggctcaa cgtgtagcta
      661 ttgccagaat gttaatgaaa ccacgtaaaa ttatcttagc agatgaacct acaggagctt
      721 tagatggtga gattggaaaa gaaattattc gtttactatt gaatgaaaca gctgaagata
      781 aatatgtcat tatagcgaca catgatccag ctgtctataa cgaagttgat gtgataattg
      841 acatgaaaga tatagggtat aaggtatgaa aaagaaaatt tatattgctt tgatctttgt
      901 aactggggta ctcgccattt tcttttttgg aaaacaaatg attacaaaag aaaatatcaa
      961 taagccaacg gtagagttaa caatctatac gctttcttct agtgatacag aaaaatggaa
     1021 taaagtgaga caagtagaaa cggaagaagc tatatacttc ataactgtga aagaagtatc
     1081 atcttcagaa gaagtatttt caaacatcat tgcaaatgga gctgctacgg ggttcggagt
     1141 tcgtgaagaa gaagtgaaga aattcaataa tggtttagga gacaccatag aggactctaa
     1201 gcataataaa ctaattgaaa tagaattttt tactttttct gatgatggcg cgggatttgt
     1261 ggtagcaaac tttgactacg gtaaagaaga gttgaatt
//
LOCUS       DAVKJD010000147         1270 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVKJD010000147 DAVKJD010000000
VERSION     DAVKJD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.147"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..359
                     /locus_tag="VW157_001982"
     CDS             <1..359
                     /locus_tag="VW157_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9553225.1"
                     /translation="NKRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITG
                     PIACISFLAGPIALNIGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLG
                     SPYLIYLLIKMNRRNI"
     gene            359..1153
                     /locus_tag="VW157_001983"
     CDS             359..1153
                     /locus_tag="VW157_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9553226.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHINS"
BASE COUNT          462 a          209 c          211 g          388 t
ORIGIN      
        1 ttaataaaag actagaaatt attgaacttg gtgaagaaat agcaatcgga cttggagcaa
       61 atcccgagct ttcaaggctt gttttaattt tttgcgctgt atctttaact gctttttcta
      121 cttcaattac aggaccaata gcttgtatat cttttttagc tggtcccata gccttaaata
      181 ttggcaagaa aagaagccca atattagctg gattggttgg aattttacta gttttgttat
      241 cagacatatt ctctcaaaat attttaccag ctagatatcc agtaggtgtt gtaactggct
      301 tgttaggttc accatactta atatacttac taataaaaat gaacaggagg aatatataat
      361 gaaaggtctg tggtcaaata atcttacctg cggttatgat gaaaaaataa tcttggaaaa
      421 tataaatata aaaatacctg aagaaaaaat atcagttatt attgggtcaa atggttgtgg
      481 gaaatcaaca ctcattaaaa ccttgtctcg acttataaag ccattagagg gagaagtatt
      541 gcttgataat aaatcaatta attcttataa agaaaaagat ttagcaaaac acatagctat
      601 attacctcaa tctccaataa tccctgaatc aataacagta gctgatcttg taagccgtgg
      661 tcgtttcccc tacagaaagc cttttaagag tcttggaaaa gatgaccttg aaataataaa
      721 cagatcaatg gttaaggcca atgttgaaga tctagcaaat aacctagttg aagaactttc
      781 tgggggtcaa aggcaaagag tatggatagc tctagcccta gcccaagata caagtatcct
      841 acttttagat gagccaacta cttacttgga tatctcatat caaatagaac tattagacct
      901 cttgactgat ctaaaccaaa aatataagac aaccatttgc atgattttgc acgatataaa
      961 tctaacagca agatacgctg attacctatt tgcaattaaa gaaggtaaac ttgttgcaga
     1021 gggaaagcct gaagatatac taaatgataa actagttaaa gatatcttta atcttgaagc
     1081 aaaaattata cgtgacccta tttccaattc gcctctaatg attcctattg gcaagcacca
     1141 tattaactct taataattga atattggaat tcatttaaaa gtcgcaaatt ttgaactgac
     1201 cctcaaaagt taggccaaaa aactaactaa aggaggtcag ttttttaatg gcaaatacag
     1261 cacagaattt
//
LOCUS       DAVKJD010000148         1265 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVKJD010000148 DAVKJD010000000
VERSION     DAVKJD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.148"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..1095
                     /locus_tag="VW157_001984"
     CDS             <1..1095
                     /locus_tag="VW157_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEU9553227.1"
                     /translation="KELSADAYFPKLLEGGQLASQPTLSRFLSRTDEETVHSLRCLNL
                     ELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEGVAYNAHYRAHGYHPLYAFEGKTGYCF
                     NAQLRPGNRYCSEEADSFITPVLERFNQLLFRMDSGFATPKLYDLIEKTGQYYLIKLK
                     KNTVLSRLGDLSLPCPQDEDLTILPHSAYSETLYQAGSWSHKRRVCQFSERKEGNLFY
                     DVISLVTNMTSGTSQDQFQLYRGRGQAENFIKEMKEGFFGDKTDSSTLIKNEVRMMMS
                     CIAYNLYLFLKHLAGGDFQTLTIKRFRHLFLHVVGKCVRTGRKQLLKLSSLYAYSELF
                     SALYSRIRKVNLNLPVPYEPPRRKASLMVH"
BASE COUNT          369 a          277 c          261 g          358 t
ORIGIN      
        1 aaagaattgt cagctgatgc ctactttcca aaattgttgg aaggagggca gcttgcttca
       61 cagccaacct tatcccgttt tctttccaga actgacgagg aaacagtcca tagtttgcga
      121 tgcctcaacc ttgaattggt cgaattcttt ttacagtttc accagctaaa ccaactcatt
      181 gtagatatcg attctaccca tttcacaact tatggcaagc aagaaggtgt tgcttataac
      241 gcccactatc gtgctcatgg ctatcatcct ctttatgctt tcgaggggaa gacaggttat
      301 tgtttcaatg cccagcttcg tcctggtaat cgttattgtt ctgaagaggc agacagcttt
      361 atcacacctg ttttagaacg gtttaatcaa cttctctttc gaatggatag tggctttgcg
      421 accccaaaat tatacgattt aattgaaaaa acagggcaat actacctcat aaaactcaag
      481 aaaaatactg ttctgagccg tcttggagac ctttccctcc cttgcccaca ggatgaggac
      541 ttaaccatct tgccccactc cgcctactca gaaactctct atcaagcagg atcttggtcg
      601 cacaagcgtc gtgtctgcca gttctctgaa cgaaaagaag gaaacttgtt ctacgatgtt
      661 atttctctcg ttacaaatat gacgagtgga acaagccaag accagtttca gctttatcgt
      721 ggacgtggtc aagccgagaa tttcatcaag gagatgaagg agggattttt tggcgataaa
      781 acggatagtt caaccttaat caaaaacgaa gttcgtatga tgatgagctg tatcgcctac
      841 aatctctatc tttttctcaa acatctagct ggaggtgact tccaaacttt aacaatcaaa
      901 cgcttccgcc atctttttct tcacgtggta ggaaaatgtg ttcgaacagg acgcaagcag
      961 ctcctcaaat tgtctagtct ctatgcctat tccgaattgt tttcagcact ttattctagg
     1021 attagaaaag tcaacctgaa tcttcctgtt ccttatgaac cacctagaag aaaagcgtcg
     1081 ttaatggtgc attaaagaac agtcgagatg aaaaaatcgt gtgacgaacc aagggaggag
     1141 tctgcccttt tgaggaaatc tagcgaggaa aaacgatact ggaacagcag aaagtaaaac
     1201 tgacctcatg aggaggaaga aagtggctca tgaggtcagg ggttttgtaa gttacatcta
     1261 gttga
//
LOCUS       DAVKJD010000149         1250 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVKJD010000149 DAVKJD010000000
VERSION     DAVKJD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.149"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            186..455
                     /gene="blpJ"
                     /locus_tag="VW157_001985"
     CDS             186..455
                     /gene="blpJ"
                     /locus_tag="VW157_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEU9553228.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            524..772
                     /gene="blpK"
                     /locus_tag="VW157_001986"
     CDS             524..772
                     /gene="blpK"
                     /locus_tag="VW157_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HEU9553229.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            1016..1165
                     /gene="blpO"
                     /locus_tag="VW157_001987"
     CDS             1016..1165
                     /gene="blpO"
                     /locus_tag="VW157_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9553230.1"
                     /translation="MNTKMMSQFSVIDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
BASE COUNT          391 a          150 c          290 g          419 t
ORIGIN      
        1 ttaagatatt ttagatactc aagattattg aaatcttaga ctaaaaaata ggtactgcta
       61 cctttaagaa gatagtagac gtatatactt ttttaagaaa atcaaaaaga tactaaaaaa
      121 aatctatttg tttattgaat ttaagacttt ggtaacaaat tgaaaataaa aaggaggtat
      181 tcatcatgaa tacaaaaatg ttgtcacagt tagaggttat ggatactgaa atgcttgcga
      241 aagttgaagg ggggtatagc tccactgatt gtcaaaatgc actgattaca ggagtcacta
      301 cgggaattat aactggtgga actggagcag gtttggccac tttaggagta gctggacttg
      361 ctggtgcatt tgttggagca cacattggtg caattggggg tggattaaca tgcttaggag
      421 gtatggttgg tgataaattg ggactaagtt ggtaagactt atagtaaaat tatgaggatt
      481 ctattctgat ttattttagt tttttaaaaa aggagtattt gtcatggata caaaaatgat
      541 gtcacaattt tctgttatgg atactgaaat gcttgcttgc gttgaaggtg gcggatgcaa
      601 ttggggagat tttgccaaag caggtgttgg aggagcagct gtagttgctg ctttaggttg
      661 tgcagcgggt ggagtgaaat atgggaaaat tctaggacca tggggcgctg caataggagg
      721 aattggagga gcagtggttt gtggatattt agcctatacc gctacatcat aaaaatgaaa
      781 gctaaatttt atgaaggcta tattctttat tattctcttc gcttttcaaa cctatcttat
      841 ttatctatcc ataagtatct ctgataaaaa acaaaagact attgaattaa catcattgaa
      901 ttgttttgtc atattatttc taatctatga taaattaatt tttctcttta ttgcctatgt
      961 ttttttgata atttttatat taaacttgtt ccgtaactga aaagtgacat actatatgaa
     1021 tacaaaaatg atgtcacaat tttctgttat agataatgaa atgcttgctt gcgttgaagg
     1081 tggagatatt gattggggaa gaaaaattag ttgtgcagca ggggttgcat atggcgcaat
     1141 tgatgggtgt gcaacaacgg tttgacattt ctattgggac catttgctat aggaataggt
     1201 gtaactggtg ctgcaggtgg agctatactt ggaggtgtag cctatgcagc
//
LOCUS       DAVKJD010000150         1162 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVKJD010000150 DAVKJD010000000
VERSION     DAVKJD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.150"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            55..495
                     /locus_tag="VW157_001988"
     CDS             55..495
                     /locus_tag="VW157_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9553231.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            492..950
                     /locus_tag="VW157_001989"
     CDS             492..950
                     /locus_tag="VW157_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9553232.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
BASE COUNT          364 a          266 c          204 g          328 t
ORIGIN      
        1 aaggatatac aggaagtgat cacaaccttg tataactgaa aaggagagaa aactatgaaa
       61 gtagaactac agattagtga gacttacgag gaggaaaagc tgattgtcca agcacctcaa
      121 gaaaccgaaa aagtccagaa agtcatcgag ttcgcagaaa atctggacca aagagaaaaa
      181 atcaaaggaa aaatcgatga tcaggtctat ctagttgaga ttggcaagat tcagcgcttc
      241 tatatcgaga atcggaaggt tctagcagaa accgcgagtc agacctacag cattgatttg
      301 cgactctatc aggttcttaa actcttgcca accaatttta tccaaatttc ccaatcagaa
      361 atcatcaata tcgattccat ctctcacctc aagctcacgc caaacggtct ggtagaaatt
      421 ttcttgaaaa acgaaagctt cacctactct tcacgccgtt atctaaaaac catcaaggag
      481 aaattagaac tatgaaaaaa caagtatttc acgatgcagc taccggtgtt cttatcggcc
      541 tcatcctctc tatcctcttt tcactcattt atgcaccaaa tacctacgca ccactaaatc
      601 cctactctct catcggccaa gtgatggatc agcatcaggt tcacggtgcc ctggtcttgc
      661 tctactgcac acttatctgg gcaaccatcg gtatgctctt caactttggc aaccgcttat
      721 ttagccgtga ctggagcatg cttcgtgcca ctctgactca tttcttcctt atgctggctg
      781 gctttgtccc actagcaact cttgctggtt ggttcccttt ccactggatt ttctacctcc
      841 agctcattat cgagtttgcg attgtctatc tcatcatctg ggctattctc tataaaagag
      901 aggctaaaaa agtagatcac atcaatcaac tcttggagca tagaaaatag gaacctcata
      961 ataagcaaat ccgagaaaga aacttctcgg tttttcttta tcctgctgca ccttactata
     1021 gtgaaatgaa ataagaacag gataaatcga tcaggacagt caaatagatt tctaacaatg
     1081 ttttagaagc aaaggtgtac tattctagtt tcaatccgct atatcataat agttatttca
     1141 gatcttcttt gtaaattttc ta
//
LOCUS       DAVKJD010000151         1141 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVKJD010000151 DAVKJD010000000
VERSION     DAVKJD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.151"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..923)
                     /locus_tag="VW157_001990"
     CDS             complement(<1..923)
                     /locus_tag="VW157_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9553233.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMKELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     KGEILEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFY"
BASE COUNT          338 a          273 c          201 g          329 t
ORIGIN      
        1 tagtagaaac ctgttgccaa ttcacgttgg gcaaccttcc acatctcgtc caccaagtct
       61 tgtttgatag cttcaaactt ttcaggactt tcaagatagg catccacagc cgccttgtag
      121 cagttggtta ctgttgatac gtagtgaata gacttcatac gtccttcgat ttttagactg
      181 tccacaccat tttcaatcat atctggaatg tggtcaatca tagacatatc aacggctgac
      241 attgaaaatt cttctagaat ctcacccttc aaactcttac gttctttccc aaatggcata
      301 tcgtaaaggt cgtatttcca acggcatgac tgagaacacc caccacggtt ggcatcacgc
      361 atactcatgt ggtttgaaag agtacaacgt ccagagtatg aaatacacat agctccatgg
      421 acaaaggctt caatttcaac atctgtacgt ttgcggattt cagctaattc tttcattgaa
      481 acctcacgtg ctaaaacgac acgagtcaag cctagctctt tccagaactc aagggtttca
      541 tagttagtgg cactggcttg ggtagaaagg tggatttcaa ggcctggtgc ttcagtcgct
      601 gcaatcataa tcaaggctgg gtcagatacg ataactgctg caatcccgat atcacgcagt
      661 ttacggaacc actcaccagc accagcttca tttccttcgt gcataaccat attagccgct
      721 acatagacct tggcaccata cttggccgca aactgcacgc cttcttccat ctgttcgaaa
      781 gtaaagtttc ccgcacggct acgaagacca taggcctgac caccgataaa gacagcatct
      841 gctccatact gaacagctac ctttagcttc tctaaagtcc ctgcaggtga taaaacctca
      901 ggacgtttta atgtttttgt catgatttct cctatttgca atataaaagt ttgacatttt
      961 tccttctcat tttatagtaa ataaaggata aaatcaagcc tagacggatt gttttgacaa
     1021 ttctaatctt ttagactttg cataataact attaaacagt cgaaaattac cccagtttta
     1081 acaagtagtc aaaaagtcta aaaaagtaaa aaccagtatc ccaagatact gactcgtaaa
     1141 a
//
LOCUS       DAVKJD010000152         1071 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVKJD010000152 DAVKJD010000000
VERSION     DAVKJD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.152"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..>1071
                     /locus_tag="VW157_001991"
     CDS             <1..>1071
                     /locus_tag="VW157_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9553234.1"
                     /translation="HQAQTVKENNRVSYIDGKQATQKTENLTPDEVSKREGINAEQIV
                     IKITDQGYVTSHGDHYHYYNGKVPYDAIISEELLMKDPNYQLKDEDIISEIKGGYVIK
                     VDGKYYVYLKDAAHADNVRTKEEINRQKQEHSQHREGGTSANDGAVAFARSQGRYTTD
                     DGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNELSASELAAAEAFLSGRKNLSNLRTY
                     RRQNSDNTPRTNWVPSVSNPGTTNANTSNNSNTNSQASQSNDIDSLLKQLYKLPLSQR
                     HVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEERIARIIPLRYRSNHW
                     VPDSRPEQPSPQPTPEPSPSPQP"
BASE COUNT          364 a          238 c          215 g          254 t
ORIGIN      
        1 catcaagctc aaactgtaaa agaaaataat cgtgtttcct atatagatgg aaaacaagcg
       61 acgcaaaaaa cggagaattt gactcctgat gaggttagca agcgtgaagg aatcaatgcc
      121 gaacaaatcg tcatcaagat aacagaccaa ggctatgtca cttcacatgg ggaccactat
      181 cattattaca atgggaaggt tccttatgac gctatcatca gtgaagaatt gctgatgaag
      241 gatccaaact accagctaaa ggacgaggat attatcagtg aaatcaaggg tggttatgtt
      301 atcaaggtag atggaaaata ctatgtttac cttaaggatg ctgcccacgc ggataatgtc
      361 cgtacaaaag aagaaatcaa tcggcaaaaa caagaacata gtcagcatcg tgaaggaggg
      421 acttcagcaa acgatggtgc ggtagccttt gcacgttcac agggacgcta caccacagat
      481 gatggttata tctttaatgc ttctgatatc atagaggata ctggtgatgc ttatatcgtt
      541 cctcatggag atcattacca ttacattcct aagaatgagt tatcagctag cgagttggct
      601 gctgcagaag ccttcctatc tggtcggaaa aatctgtcaa atttaagaac ctatcgccga
      661 caaaatagcg ataacactcc aagaacaaac tgggtacctt ctgtaagcaa tccaggaact
      721 acaaatgcta acacaagcaa caacagcaac actaacagtc aagcaagtca aagtaatgac
      781 attgatagtc tcttgaaaca gctctacaaa ctgcctttga gtcaacgcca tgtagaatct
      841 gatggcctta ttttcgaccc agcgcaaatc acaagtcgaa ccgccagagg tgtagctgtc
      901 cctcatggta accattacca ctttatccct tatgaacaaa tgtctgaatt ggaagaacga
      961 attgctcgta taattcccct tcgttatcgt tcaaaccatt gggtaccaga ttcaagacca
     1021 gaacaaccaa gtccacaacc gactccagaa cctagtccaa gtccgcaacc t
//
LOCUS       DAVKJD010000153         1018 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVKJD010000153 DAVKJD010000000
VERSION     DAVKJD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.153"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(233..397)
                     /locus_tag="VW157_001992"
     CDS             complement(233..397)
                     /locus_tag="VW157_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEU9553235.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQVQEKCK"
     gene            complement(468..914)
                     /locus_tag="VW157_001993"
                     /pseudo
     CDS             complement(468..914)
                     /locus_tag="VW157_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          301 a          211 c          182 g          324 t
ORIGIN      
        1 cggactgcct ttattttttt acttaattta atgcagtaaa tcatacgatt gaaaggaatc
       61 tgcgttatat gatataatta ggtagttaat ctatacattt agaaaggtaa aatatagaat
      121 tggagaatga tatgggattt tcatataata aattgtaacc gcccaataac gaagtatatt
      181 gaaaaatctc cagactagag aactcatata tagttcctaa tctggagatt tcttatttgc
      241 acttttcttg tacttgtaca actttagtcc acggtaaata ggcctctaaa acctctttgt
      301 ttacgagagt ttcctcgttt ggaagacatt ctagaagata ggatagatat ttctcactat
      361 ttagttgatg acgtttagct gtttccaata agctcataat aatagctctt gctttagctc
      421 cttcaaaact ttgagaaaac aaccaccctc gcttttttaa actgagctta ggctaaaaga
      481 gtccactgga ctcttttact ccgtcccata accaatgatt taatggcgcg ttcagctaga
      541 ttattggaaa ggaccagatg tccgtctttt aaaatggtct taaaggtttc ttcatacttg
      601 aggctgtatt caattgccct tcctagtttt gaacccgata aaactgactg acgacggcac
      661 caagcaaaga agtcttccag taggggttgg agatgttctt gacgtttctg tagtcgttca
      721 tcagctggca aagcctccca gtctctttcc aaggaaaata actgatcaca ataagctaaa
      781 cctttagctc ctaaggatga tttatctgct tgcttggggg tcgcttcaaa aaacttcctt
      841 ctcacatgcg cccaacatcc aacaagtgca gcttcttcca gttgacgata agcttctagc
      901 tgttcgcagt ttaccaagct tagcgtcgcc ttactcctaa actttggact gctatcgcat
      961 aggcagaact agaggactaa agtcctaagt tactgccgca acatatcaca atgcacat
//
LOCUS       DAVKJD010000154          977 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVKJD010000154 DAVKJD010000000
VERSION     DAVKJD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.154"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..200)
                     /locus_tag="VW157_001994"
     CDS             complement(<1..200)
                     /locus_tag="VW157_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553236.1"
                     /translation="MLVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKK"
     gene            complement(208..579)
                     /locus_tag="VW157_001995"
     CDS             complement(208..579)
                     /locus_tag="VW157_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEU9553237.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
BASE COUNT          346 a          206 c          115 g          310 t
ORIGIN      
        1 aattttttta aaaaacttaa tagtaaattt gctccactga ttgctatcaa tatttcaatt
       61 ggtaaaaaat agtaattcca aataaaatga gaaatcagca atgatagaaa aataacaata
      121 tcaattttcc tgttgttgac atactctgta attttatgat acaggaatgc aacaataact
      181 gaaattaaaa caaccaacat aagattatta acctattctc aattgattat cacgtcgccc
      241 atcccagcca tcaataatat tggtaaaaat ccaatcagct ccagcaccaa ttccagcaga
      301 tgcaatactt ctaataacag tatttgcaaa agcatttcca atatgaattc ctaatactcc
      361 taaaccttta acaatcccag ctaccagtgc ggagcgtaat ccataagatg caagaactag
      421 ttgaaggcct ccacctcctg ctgctgctaa tgctgcatat gctgcaccag taatggctcc
      481 tgtaattgcc cacctgggta tatatatagc ccccccatca aggtacatca tctcttcttc
      541 atcaatcaca acataattat ttggtaatac aagttccatt tattttctcc ttttttatta
      601 agcatgctta ggatggtttc ctaacaagtg atcgcaggct ttgtttagtt acctaaatta
      661 gacttccttc ctttatctta ttttaaatat ttaaaaccag ttactgcttg aagcttaaca
      721 gtttacttaa ttcaaataac agctaatcac tgattccctg attttatcaa atatactaat
      781 aacaacaatt gtgatacgat catcattata agaacaacat ttaacgaatc aaagtttttc
      841 cgagataata ataaagcaac agcaagcagt attattacta aagattgaat aactactatt
      901 actcttaaca ttttatcctc cactaacaac tcaagctccc ttacattata tcgaccagcc
      961 ccatggaaag attatag
//
LOCUS       DAVKJD010000155          977 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVKJD010000155 DAVKJD010000000
VERSION     DAVKJD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.155"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(1..365)
                     /locus_tag="VW157_001996"
                     /pseudo
     CDS             complement(1..365)
                     /locus_tag="VW157_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(611..922)
                     /locus_tag="VW157_001997"
     CDS             complement(611..922)
                     /locus_tag="VW157_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553238.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
BASE COUNT          308 a          198 c          154 g          317 t
ORIGIN      
        1 tagtaaaatc ctgtagccag ttcacgttgg gcaaccttcc acatctcgtc caccaagtct
       61 tgtttgatag cttcaaactt ttcaggactt tcaagatagg catccacagc cgccttgtag
      121 cagttggtta ctgttaatac atagtgaata gactccatac gtccttcgat ttttagactg
      181 tccacaccat tttcaatcat atctggaatg tggtcaatca tagacatgtc aacggctgac
      241 attgaaaatt cttctggaat ctcaccctgc aaactcttac gttctttccc aaatggcata
      301 tcgtaaaggt cgtacttcca acggcataac tgagaacatc caccacggtt ggcatcacgc
      361 atactctaga cggattgttt tgacaattct aatcttttag actttgcata ataactgtta
      421 aacagttgaa aattaaccca gttttaacaa gtagtcaaaa taccagataa aggaaaaaag
      481 tctttaaaat cttaaaaacg catagtatca ggtattgaac aatcttgata ttatgcgttt
      541 tattgtggga agatttactt cattttctct tgaaattgaa ttttctccta cttcaaaact
      601 agatgatttg ctatttaaaa tagcgttcct tatgattttg gcaacctgga ttaaagcgag
      661 aaaaacaaaa agggcaactt aaactttctt tatattgctt atatgttagt agttttagac
      721 aaacaccaca taaaataggc ttatcctgta tcaaagataa gggatacggc tcaaaagggt
      781 gattttctaa tgaatcatga caccgataac aagcataata ctttttacaa tcatagcatt
      841 gtaaagcaat aatatccagc ttgctatggt aatgaatgca tcttgtttgc ttatccacta
      901 agaaaccctg tgcctctatc atttcctttt cctttcttaa gcacgaactg tgacgctggt
      961 tccatcttcc ttataaa
//
LOCUS       DAVKJD010000156          924 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVKJD010000156 DAVKJD010000000
VERSION     DAVKJD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.156"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..786)
                     /locus_tag="VW157_001998"
     CDS             complement(<1..786)
                     /locus_tag="VW157_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9553239.1"
                     /translation="MNFALNKGEIVGLVGRNGVGKSTLMKILVQNNQPTSGNIISSDN
                     VGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQEIFRSLIQELDLTQSINKKVKTYSL
                     GTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKNLALHENVGILISSHKL
                     EDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGDIVQEE
                     GFRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRS"
BASE COUNT          305 a          168 c          103 g          348 t
ORIGIN      
        1 tgaacgattt aggtaaatat ctttaagcgt ctctttttta gtttcaaaat caactacttt
       61 aatagagttt tcgttaaaaa atttaaaaag ctcactactt tgaatattcc cagacatagt
      121 aattctaaat ccctcttcct gaacaatatc cccaaattct tgtttggtaa tgaaaatgtc
      181 tctatctgta gctgatgaaa aagctatctc aaacaagaaa ttatgactat cttttcctac
      241 tttttgaaat gtcaaaagcc cgttctccaa gaaaagaact ctctcacaaa tttcttcaat
      301 gtcttctaat ttatgactcg atattaaaat tcccacattt tcatgtaaag ctaaattttt
      361 tagaaccgct aaaactattt gtgatgattc aatatctaaa ccattagtcg gttcatctaa
      421 aatcaatata tcaggttccg taacgagagt tagaagcaaa gctaattttt gttttgtacc
      481 caaagaatag gtctttactt ttttattaat agactgagtc aaatctaact cttggatcaa
      541 acttctaaat atttcttgat tgtagtcaac accatataaa tttgacaaat attttaaatt
      601 ctctaaacct gttttagata aaaataattt tggttcttcg attaaatacc caacattatc
      661 actgcttata atattacctg aagtcggttg attattctga acaagaattt tcatcaacgt
      721 actcttacca actccatttc tccctactag accaacaatt tcacccttgt taagtgcaaa
      781 atttatgtct ttgagaatga ttgaatgacc ataatgctta cttacattta taacttacat
      841 tttttattcc tccattaata ttcatggttt gctctttgat tcttattgag tttaaaaagt
      901 tgcgcctctt tttaacaaca ttat
//
LOCUS       DAVKJD010000157          911 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVKJD010000157 DAVKJD010000000
VERSION     DAVKJD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.157"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            81..878
                     /locus_tag="VW157_001999"
     CDS             81..878
                     /locus_tag="VW157_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9553240.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
BASE COUNT          238 a          154 c          201 g          318 t
ORIGIN      
        1 ttttatgaaa tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa
       61 aaaagattta ggagtttatt atggtttctt cagaatttat ttcaaagatt gaatttgctt
      121 gcaataagaa agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt
      181 tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta
      241 ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg
      301 cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg
      361 gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct
      421 tgttcaacct tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg
      481 cgaatctgac acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa
      541 atgaattggt tttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt
      601 catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca
      661 tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga
      721 aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact
      781 ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg
      841 ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg
      901 tgcttttttc a
//
LOCUS       DAVKJD010000158          869 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVKJD010000158 DAVKJD010000000
VERSION     DAVKJD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.158"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            1..869
                     /locus_tag="VW157_002000"
                     /pseudo
     CDS             1..869
                     /locus_tag="VW157_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic (srt) production protein"
BASE COUNT          353 a          118 c          160 g          238 t
ORIGIN      
        1 aattttttca agattatttt aaaaataagg aagtcattgc ttcagaagga ttagagaatt
       61 tacaggaaca gtaccatgct ttccaagaag aacgagataa ggataaagta tccactgaag
      121 agattaagga agcctttaag acatttaaga agcctttaag acatttaagg aaaaatgaga
      181 tactgttcgt gaatttgaag tggaacttgc agagaaccaa atagagaagc tagttgataa
      241 gggcgtctat atcaaggtgt ctttcggtgt caaatagagc ggtcttattt tcattcccaa
      301 ctatcaattg gatattatgg aagaagaaaa tcataaaaaa tataaagtct acattcgaga
      361 aacgtcttct tactttgtct ataataaaga aaacatggat aataattgtt tcatcaaggg
      421 taggacctta atcagacaat tgagcaacga tagccaaaaa ctcccctata gacgaccaac
      481 tttaaaaagt cttcaagaga agatttctga aattaatctt atgattgaac tatctaacac
      541 gaacaaacaa taccaagaaa tcaaagatga gcttgtatta gaaattgcag agattgatat
      601 gaagctagaa gaaacccaag aaaaaatcgc caccttaaac aagatggcgg aagtccttat
      661 caatttgaag agtgaagatg agatcggtag aaaattagct aagtatgatt ttgaccaaat
      721 gaacatgacg gaatcaataa tgttagatcg actcaataca gatattttaa aattacaaca
      781 ggaacttagt aatgaaatta ataaatatga ggagatagct agaagattgg atttgtttgt
      841 gaaaattatt aataccaata aatttacag
//
LOCUS       DAVKJD010000159          863 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVKJD010000159 DAVKJD010000000
VERSION     DAVKJD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.159"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            364..588
                     /gene="pneA1"
                     /locus_tag="VW157_002001"
     CDS             364..588
                     /gene="pneA1"
                     /locus_tag="VW157_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEU9553241.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKICISAAVSGIGGLVSYNNDCLG"
     gene            595..783
                     /gene="pneA2"
                     /locus_tag="VW157_002002"
     CDS             595..783
                     /gene="pneA2"
                     /locus_tag="VW157_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEU9553242.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
BASE COUNT          317 a          105 c          162 g          279 t
ORIGIN      
        1 aataaacaaa aaaaaaagtt aaaaattaca taaaaatact tgactaaata aaatatatct
       61 gttagaataa aaagaaaggg gtttcattgc atgagaaaaa aacgtggaat taaaaaatta
      121 gttacatttg cattgctagg tgtttttatg tttagtaata caattcctta ccaacagttt
      181 attcagaaga ataaacaatt ggagattcga gtgcaatcgc aaaagaagtc caatggtctt
      241 gatgttggga aggcggatta aaacccgata ttttgcacga gaatgagaga attataattt
      301 atctgaaaaa acgaaaaata tagagcaact tatttaactt tttataagga ggagttatta
      361 cttatgacca actttaattc aaacgaaaaa ttttgtggaa aaagtttaaa atcattgtct
      421 gctgatgaaa tgagtttaat ttatggtgca tctgatggtg cagagccacg ttggacgcct
      481 actcctatta ttttgaaatc agctgcagct agctcgaaaa tatgcattag tgcagctgtt
      541 tctggtattg gggggctagt tagctacaat aatgattgtt tggggtaaat taaaatgaaa
      601 aatgattttg taatcggaaa gagtttgaaa gaattatctc ttgaagaaat gcaattggtt
      661 tatggtggca cagatggtgc tgaccctaga tcaacaatta tatgttctgc tacattaagt
      721 tttattgcat catatttggg gtcagctcag actcgttgtg gcaaagacaa caagaaaaaa
      781 taaaatattc aattttaaaa ataaaagttg ctctttattt tcctctctcg taactaataa
      841 ttttgagaga ggtttttttt taa
//
LOCUS       DAVKJD010000160          851 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVKJD010000160 DAVKJD010000000
VERSION     DAVKJD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.160"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(160..240)
                     /locus_tag="VW157_002003"
                     /pseudo
     CDS             complement(160..240)
                     /locus_tag="VW157_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            248..610
                     /locus_tag="VW157_002004"
     CDS             248..610
                     /locus_tag="VW157_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU9553243.1"
                     /translation="MLEVGLNSLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYPYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT          270 a          132 c          156 g          293 t
ORIGIN      
        1 cgaaatctag tggaatgaat ctggaatagt ccatcgagct ttctaatact ctttgaaaat
       61 ctcttcaaac cacgtcaacg tcgccttgcc gtgcgtatgg ttactgactt cgtcagttct
      121 atccacaacc tcaaaacagt gttttgagct gactacgtca gttccatcta caacctcaaa
      181 acagtgtttt gagcaacctg cggctagttt cctagtctgc tctttggttt tcattgagta
      241 taacacattg ttagaagttg gtttaaattc cctaatcagt ttgttcacat ttaccttcga
      301 tatattatat cccatagtta aggttggtca tacagatgat tatagtcatg gagccgtaaa
      361 acttagtgtt tctttagttg acaaagatgc catgaaaaaa atatctgtaa ctgtaatagg
      421 atattttgaa ataaatatag atgaaaatat caccgatatt ctatacgtaa atggtactgc
      481 tattctttat ccttatttac gttctattgt ttcaatagtt tcggcaattg atagcagtga
      541 agcaatgttg ctacctacca ttaatgtttt agagttacta gataaatctc aaccttttga
      601 agaagaataa tttattagct cactaaattg agggtaagga aaagtaaaat cagtaagaaa
      661 aatgtcttgc attatacagc aaccttttgg gaatgagtag atggattgaa taaaatttga
      721 ttaagagtgg atgatttatc tgtagattat tattggacag ttagtcttga agtagtctaa
      781 gaattaggtt ataatcagta gaagccttgc taataatgag gaggttagtt tatgtatagt
      841 agactgaatc t
//
LOCUS       DAVKJD010000161          811 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVKJD010000161 DAVKJD010000000
VERSION     DAVKJD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.161"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
BASE COUNT          304 a          154 c           91 g          262 t
ORIGIN      
        1 tctgtaagtc aatatttgat aaagcaaagc tttaacccct tccccagacg ttagcttttc
       61 ttcaacccac tacagttgac aaggagccca aattattgat aaaaacttta gtcatcatcg
      121 tctctataaa aatattttct atacactctt gataatacca aaattaatgc caatatcata
      181 ccaagagcta taataataga atttattaga tttattttag tatatccaat cactagtcca
      241 ataattggac tagcagacat gcttagatat aataaaaata acaaaaaatg aatttcacca
      301 attcctgcaa aaaaatgttt catagctata gttttacgcc cataaatacc catcaaattt
      361 accgtctacg atatctaaac catatgcaat ggctcctcct aatgagaaga cttcagcaac
      421 agtagccgca atattaagag cattagtaat agttgccata ctaactccac caatatatct
      481 tgttatagca cttagttacc tagataagat atcctttagc ttgcttccaa aatttaagtc
      541 tgcacctata atatgagttc atattagtac tgctaaatcc tatctatagt aatgtcaaac
      601 acctaaataa agtcatctct ccaattcatt caagaaatga agaaaggcaa aaaatcttta
      661 aaataagaaa agtgcatatt atcaggtgtt caaaaatctt gataatatgc gttatataat
      721 agaaaaattt actcaatttc attttcaaat tgagtttttt cccagcctca tttttaattt
      781 atatcttcaa tagaatctaa tgaccgagct a
//
LOCUS       DAVKJD010000162          797 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVKJD010000162 DAVKJD010000000
VERSION     DAVKJD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.162"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            322..621
                     /locus_tag="VW157_002005"
     CDS             322..621
                     /locus_tag="VW157_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9553244.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          308 a          145 c          131 g          213 t
ORIGIN      
        1 aaataataat agacaactat ttatccaaaa atagatagat gtaacatgtt tgcaaacaaa
       61 gcatacgagc ctttagtaaa atcatttcca tgaaactaga atagagccct cttagcaaaa
      121 atcattattt taatttattt ctaatcactc cttgacataa ataactctca ccaataaaag
      181 actatgtctt aaaaaaatgg tataataaaa tcaatacttg ggcttgatgg ctatgctact
      241 aataacaatt aggagagaaa atcaggcact tgttaacaac aaggattatc cccttgagat
      301 gaaaggaact ttagaaatct tatgatgaac atgcaaaaca tgatgcgtca agcacaaaaa
      361 cttcaaaaac aaatggaaca aagccaagct gaacttgctg ctatgcaatt tgttggcaaa
      421 tctgctcaag atcttgtcca agcgacctta actggcgata agaaagttgt cagcattgat
      481 ttcaatccag ctgtcgttga cccagaggac cttgagactc tttctgatat gaccgttcaa
      541 gccatcaact ctgctcttga acaaatcgat gaaactacca agaaaaaact gggtgctttc
      601 gctgggaaat tacctttcta aaaacaagga gctagaacaa tgcttgtcga taacaaaggc
      661 taagaaaggt gcaaaaatga ctctataata tttgtagtgg gtaaatccac tatagatatt
      721 atggagccta ttttattgta gaaaaaaagt cccatatgac ctataatgaa aagcgaccaa
      781 acaactcatt agaaaga
//
LOCUS       DAVKJD010000163          782 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVKJD010000163 DAVKJD010000000
VERSION     DAVKJD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.163"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
BASE COUNT          291 a          134 c          107 g          250 t
ORIGIN      
        1 ttgattgaat ctaactaaag gatcctattc aattactaga actatcacat actcaaggtc
       61 agctcacaga tgagcaacta ttttggttac aatgtctact aaatttaagt caaacaaata
      121 atttagtcaa aattaaaaaa atagaggaac ataaatatga ttacaaaaca gaatgtaata
      181 gtgttctacg atttttacta gataaaactg taaattctga aggaaggatc acttcttcaa
      241 cagaatttgg aaatttcgta agtaatttat cattccaaca cggaatagct ggactactgt
      301 ttcctctaaa taaattgtac cccccagaac tggattctaa aatactctct atcatcaaga
      361 aggcagtgac aattagaacg acacacacat atgaatatca atactcactg ctatttggtg
      421 atgcaggcta tctatagtta ctcctacatt tattttctat cagtaaaaat caatactatc
      481 tacaattagc aaacgtcacc gctaagaaat taatagagaa ttatgatact ctagaggaaa
      541 tagactttgc attgggaaaa tctggtgtcc tattatcatt aataaaatac tatcatttta
      601 ccaatgacaa tactcttaaa actttcatcc acaatagtat atggggaaat ttatcattat
      661 ttcctacaaa gagatacagc caaagaaagc attttagact atagctttgc tcatggatat
      721 tgtggaattg catatgcttt atttgcctat tctaaagtct tagaaccttc tatgttctat
      781 aa
//
LOCUS       DAVKJD010000164          765 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVKJD010000164 DAVKJD010000000
VERSION     DAVKJD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.164"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(137..>765)
                     /locus_tag="VW157_002006"
     CDS             complement(137..>765)
                     /locus_tag="VW157_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9553245.1"
                     /translation="TVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLAT
                     VKYYVEHPNERPHSDNGFGNASDHVRKNKADQDSKPDEDKEHDEVSEPTHPESDEKEN
                     HAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTD
                     SSIRQNAVETLTGLKSSLLLGTKDNNTISAEVDSLLALLKESQPTPIQ"
BASE COUNT          206 a          157 c          126 g          276 t
ORIGIN      
        1 gttatttaac catttaatta accagtaaat agtttactat ttaaaattac ttttgtcaag
       61 tttctgttac gtttttctaa aaaagagaag taacacgaaa ggttacttct ccataaaata
      121 tgctccattc attttactac tgtataggag tcggttgact ttcttttaac aaagccaaga
      181 gactatctac ttctgctgaa atagtgttat tatctttcgt tccgagaaga agactacttt
      241 ttagaccagt caatgtctcc acagcatttt gtctaatact agaatctgtt actttttcta
      301 gcaaggcctc cgcctctgct atcttagcgt taataacaga atgctctact tgaggaattt
      361 cagcctcatc tgtagtatct tcagcttctt cctctgtctc ttccgtatca gtgcttggtt
      421 tataaagatt atctgctgaa ggatttaaac cagcgtgatt ctctttttca tcagattcag
      481 ggtgagttgg ctcacttact tcatcatgtt ccttatcttc atcaggttta ctatcttggt
      541 ctgccttatt tttacgaaca tggtcgctag cgttaccaaa accattatct gaatgcggac
      601 gttcgtttgg atgttcgaca tagtacttga cagtcgccaa aagatcctca agagtatacc
      661 ccttaggtgc ctcataaagg ccttcgtcaa accactcaaa tttgatgtta tggtaatggt
      721 cataatgagg tatgattaaa ctaccgtttt tgacttccac agtat
//
LOCUS       DAVKJD010000165          676 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVKJD010000165 DAVKJD010000000
VERSION     DAVKJD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.165"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            4..183
                     /locus_tag="VW157_002007"
     CDS             4..183
                     /locus_tag="VW157_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9553246.1"
                     /translation="MTSEELKSLGKWYVSTGKEWICHSDDELEEFKNLFLNFINPEEW
                     DTISFDSDFMPFQQS"
     gene            complement(331..663)
                     /locus_tag="VW157_002008"
     CDS             complement(331..663)
                     /locus_tag="VW157_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553247.1"
                     /translation="MSNTSYTNYPSHVRSSVEKMNQVFDTPAKRKFVANKARLYFSDK
                     TKSDVYSEAYAYLLNSLGVNTNIQDEFTQKLVAFYFEGQKRYCNGTSMLDDFIKYGKL
                     PHHFSSYK"
BASE COUNT          215 a          104 c          111 g          246 t
ORIGIN      
        1 tgtttgacga gtgaagaatt gaaaagtctt ggaaaatggt atgtctcgac tggtaaagaa
       61 tggatttgtc attcagatga tgagctggaa gaatttaaaa atctattttt aaattttatc
      121 aatcctgaag aatgggatac tatctccttt gattcagatt ttatgccgtt tcaacaatcg
      181 taaccaattt ctcaaaaaag ttaaatctta tatttagtac tctgtaaaac tcttatctaa
      241 tcacgttgct tatactcaga agtaatattg cttattcatt atatgtctcc ttttttatta
      301 ataaacggga gggaagtcct ccccatgaaa ttatttatag ctactaaaat gatgaggtaa
      361 tttcccatac tttatgaaat catctaacat agaagtaccg ttacagtatc tcttttggcc
      421 ttcaaaatag aaggcgacta atttttgtgt aaattcatct tgaatatttg tgtttacacc
      481 taatgagttt agcaaatagg cgtatgcttc actataaaca tcagatttag tcttatctga
      541 gaagtaaaga cgtgctttat ttgcaacaaa tttgcgtttt gcaggggtat caaagacttg
      601 attcattttc tcaacggaac tccgaacatg actaggatag tttgtataac ttgtatttga
      661 catagtattt ctcctt
//
LOCUS       DAVKJD010000166          675 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVKJD010000166 DAVKJD010000000
VERSION     DAVKJD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.166"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..313)
                     /locus_tag="VW157_002009"
     CDS             complement(<1..313)
                     /locus_tag="VW157_002009"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEU9553248.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HN"
     gene            complement(303..>675)
                     /locus_tag="VW157_002010"
     CDS             complement(303..>675)
                     /locus_tag="VW157_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEU9553249.1"
                     /translation="DILQLLSDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTY
                     ELKMQDATISSSELEFLCDLLLYKTLDQGRYNVEGPLVLARYLLGCEFEVKNLRMIIS
                     ALQNTIPFESIKERIRPHYGS"
BASE COUNT          223 a          117 c           92 g          243 t
ORIGIN      
        1 tattgtgtcc tactgctttc tctacattat cctctatacg ttttaatcct aaatttaaac
       61 cttgtttatg agtcggtaat aaaataatag caggcacaac ttgggaatca taatggcgaa
      121 ttgtatctaa tatcattgaa gcaatgtctt cagtgatata aatgacacca taatcagatt
      181 gagctaattt tcttagtgta tttatagctt cttgttcttg gtaggcagga aatatatcaa
      241 acccaatcat gctaaatggt aaaataatat cacggctacc aattatgcca attttatact
      301 tattagcttc cataatgtgg gcgtatcctt tcttttattg attcaaaggg aattgtattt
      361 tgaagagctg atatgatcat tctgagattc tttacttcaa actcacatcc caataaatat
      421 ctagcaagaa ctaacggccc ctctacattg taccttcctt gatctaaagt tttatacaat
      481 agtaaatcac ataaaaattc taactcagaa gatgaaattg ttgcgtcctg catcttcaat
      541 tcataagttg aaaagattga atctaagctt ggatttattt tattgaacca cacaaatatt
      601 tcttgatttt ctacaatgta tataaattct ttagcagaaa tacttccttc atctgaaagt
      661 aattgtaaaa tatcc
//
LOCUS       DAVKJD010000167          639 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVKJD010000167 DAVKJD010000000
VERSION     DAVKJD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.167"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..279
                     /locus_tag="VW157_002011"
     CDS             <1..279
                     /locus_tag="VW157_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9553250.1"
                     /translation="IHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQ
                     LLPFHQFGENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            354..551
                     /locus_tag="VW157_002012"
     CDS             354..551
                     /locus_tag="VW157_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9553251.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKHLHISPKELKLLGRIVK"
BASE COUNT          225 a          114 c           85 g          215 t
ORIGIN      
        1 attcattatg ctttttctca aaataaaact atcgttttaa gaatcccagt tattcctaat
       61 tttaacaata gtttagagga tgcagaaaaa ttcgctactc tatttaactc attaaatatc
      121 gaccaagttc aactactccc ttttcatcaa tttggtgaaa acaaatatcg tttattaaat
      181 cggaaatatg aaatggatgg agtcaacgca cttcatcctg aagatcttat tgattatcaa
      241 aaggtatttc tgaaccacca tattaattgt tatttctagt tcatttcctt gaaacgctct
      301 agctatttgc agataacaag catctatata aggttatcaa agaagacttc cccatgactc
      361 taaatgaata tatcttacaa tatcgcctga aacaagctat agataagatg gctgaatctc
      421 ccaactcccc tttaagcgct atctctgacc aagttggatt ttcagactat aaatattttg
      481 ccaaagtatt taaaaagcat ctccatattt ccccaaagga attgaaatta ctcggaagaa
      541 tagtaaaata ggctattctt ctttttgtag agaattttac tctaatatag tgaattgaaa
      601 taagatgtga acatctctat caggaaagtc aaattaatt
//
LOCUS       DAVKJD010000168          633 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVKJD010000168 DAVKJD010000000
VERSION     DAVKJD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.168"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            8..633
                     /locus_tag="VW157_002013"
                     /pseudo
     CDS             8..633
                     /locus_tag="VW157_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          217 a          117 c          133 g          166 t
ORIGIN      
        1 tagttctatg aatgatgaag caagtaaaca actaactgat gcacgattta agcgtcttgt
       61 tggtgttcaa cgcacgactt ttgaagagat gttagctgta ttaaaaacag cttatcaact
      121 taaacacgca aaaggtggac gaaaacctaa attaagccta gaagaccttc ttatggccac
      181 tcttcaatat gtgcgagaat atcgaactta tgaagaaatt gcggctgatc ttggtattca
      241 cgaaagcaac ttaatccgtc ggagccaatg ggttgaagta actcttgttc aaagtggttt
      301 tacgatttca agaactcctc tcagttctga ggacacggta atgattgatg cgacggaagt
      361 acaaatcaat cgccctaaaa aaagaattag cgaatgattc tggtaaaaag aaatgccacg
      421 ctatgaaggc tcaagcgatt gtcacaagtc aagggagaat tgtttctttg gatatcactg
      481 tgaactatag tcatgatatg aagttgttca aaatgagttg cagaaatatc ggacaagctg
      541 gtaaaatctt ggccgacagt ggttatcaag ggctcatgaa gatatatcct caagcacaaa
      601 ctccacgtaa atccagcaaa ctcaagcaaa ctc
//
LOCUS       DAVKJD010000169          625 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVKJD010000169 DAVKJD010000000
VERSION     DAVKJD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.169"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..608
                     /locus_tag="VW157_002014"
     CDS             <1..608
                     /locus_tag="VW157_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9553252.1"
                     /translation="EGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNGRGKS
                     TLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVK
                     NFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFVFFDEPSSGMD
                     YSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADEIFEL"
BASE COUNT          243 a           72 c          121 g          189 t
ORIGIN      
        1 aagaaggtgg agagtatcag ataaaaaatc ttagctacaa atttactgat gatgagtgtt
       61 taagcttaaa agatatttcg ttcaagcttg ggaaaattta tggcataata ggatccaacg
      121 gacgaggaaa atcaacgctt ttaagatgtt taataggtct tgagaaaaaa tcaaaagaag
      181 aaatttattt taagggagag aagctatcta aaaaagaaag actcaaaaac tcttcacttg
      241 ttatgcaaga tgtaaatcat caattattca cagatgaagt attcaacgag cttagattag
      301 gagtaaagaa ttttgatgaa gaaaaggcga aaatcatctt aaaagattta ggcctggacg
      361 aatttattga aaggcatccg atgagtttat caggagggca aaagcaaagg cttgcaatag
      421 catctgttat gtgcaagaat tctccatttg tcttttttga cgaaccttca agtggtatgg
      481 attattccaa tatgataaaa atatctgaac tgattaataa gtataaaacc atggataaaa
      541 taatttttat tgtttcccat gatatagaat ttttaaatga agtggcagat gaaatttttg
      601 aattgtaaaa tagagtctgt taaac
//
LOCUS       DAVKJD010000170          624 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVKJD010000170 DAVKJD010000000
VERSION     DAVKJD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.170"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            165..>624
                     /locus_tag="VW157_002015"
     CDS             165..>624
                     /locus_tag="VW157_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9553253.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYI"
BASE COUNT          229 a           92 c          110 g          193 t
ORIGIN      
        1 aaaaatgctt ggagtatttg tttgtgtgtt tatttttata taacaaacta taaacaaaac
       61 aaaaatataa aaaaagagac aaaaaagaac agaaagtaat tgacaacggt ttcatatagt
      121 gttatactta tggcataaag ttaatggaaa gaagggaaaa acttatggta ttagattatt
      181 tctttgacaa aaaccttgtg ttttgcttag aagcggataa tcaagaacat ctctttgatc
      241 aggttgcaac tttattggaa gaacgagaaa ttgtaactcc aacttatcgt gaagccttga
      301 tcacgcgtga aaagtcattt ccaactggtt tagatatgga atttctagga aaggacttgc
      361 caaatgtagc gattcctcat acagatattg ttcataatct agctgagaaa gtggtggttg
      421 ttcgattaga aaaaccagta acttttcaca atatgatagc tcctgataag gaagtagaag
      481 tatccctact cttctttatc attaacaatt caagttcaag tcaaacaaat attctggctc
      541 agttgatgga ctttttcaca ggaaatggac atcttgaaga cctatcaaaa atttccgaac
      601 cagaaaaact ttatgcttac attg
//
LOCUS       DAVKJD010000171          599 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVKJD010000171 DAVKJD010000000
VERSION     DAVKJD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.171"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            175..381
                     /locus_tag="VW157_002016"
     CDS             175..381
                     /locus_tag="VW157_002016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9553254.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KKTLTPTKESIDFLRNKIMKQVNN"
     gene            315..>599
                     /locus_tag="VW157_002017"
     CDS             315..>599
                     /locus_tag="VW157_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEU9553255.1"
                     /translation="MDPYQGVNRLPAKQNYEASKQLTDARFKRLVGVQRTTFEEILAV
                     LKTAYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNL"
BASE COUNT          241 a          103 c          104 g          151 t
ORIGIN      
        1 aagtcaagga aaattaaccc actttaattt cttgtttaac ccgggttaaa aaagaaatta
       61 aagtgggtta aaaaagaaat taactgagta aaaactctcg aaattcaatt cgctatatta
      121 tcactcaact tgaacaaata ccagaaccac aaaaaacaga tgtaaaggaa aaatatggac
      181 ttaaaatttg aaggggttga tttagaatac aaaaaagcta aaaacaatct tccagagtct
      241 ttctgggaaa cctactcagc atttgcaaat acaaatggcg ggaaaataat tctcgggata
      301 gatgaaaaaa aaacattgac ccctaccaag gagtcaatag acttcctgcg aaacaaaatt
      361 atgaagcaag taaacaacta actgatgcac gatttaagcg tcttgttggt gttcagcgca
      421 cgacttttga agagatatta gctgtattaa aaacagctta tcaacttaaa cacgcaaaag
      481 gtggacgaaa acctaaatta agcctagaag accttcttat ggccactctt caatatgtgc
      541 gagaatatcg aacttatgaa gaaattgcgg ctgattttgg tatccacgaa agcaactta
//
LOCUS       DAVKJD010000172          529 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVKJD010000172 DAVKJD010000000
VERSION     DAVKJD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.172"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            1..529
                     /locus_tag="VW157_002018"
                     /pseudo
     CDS             1..529
                     /locus_tag="VW157_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          161 a          111 c           89 g          168 t
ORIGIN      
        1 tcaattgaag aaatatgact ttcaaaaacc ttctaaaatc ccttacctcg aaacaactgg
       61 tatgccttct agaattctcc ttagaaagcg tcgattcaag tgctatcact gttcaaaaat
      121 gatggtcgct gaaacttcta tcgtcaagaa aaatcaccaa atccctcgta tcatcaacca
      181 aaagattgct caaaagttaa ttgaaaagat ttctatgact gatattgccc atcagctttt
      241 catctcaact tcaactgtta ttcgtaagct caatgatttt cactttaaac atgatttttc
      301 ttgtcttcct gagattatgt cttgggacgt tgaaacagtc cgggtagtga ctgtttcaat
      361 cgggagatgg agatgagctt tattgcgcaa gattttgaca agctcaatat catcactgtt
      421 cttgagggaa gaacacaagc tatcattcga aatcactttc ttcgctacga tagagccgtc
      481 cgatgtcgcg tcaaaatcat tactatggat atgtttagcc cttactatg
//
LOCUS       DAVKJD010000173          524 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVKJD010000173 DAVKJD010000000
VERSION     DAVKJD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.173"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(<1..469)
                     /locus_tag="VW157_002019"
     CDS             complement(<1..469)
                     /locus_tag="VW157_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553256.1"
                     /translation="MVRDVIPINLVQEYCQGQPYGLLKKMLKRIRLSREIALLLATVI
                     VYFFTHRILPLSVFTFMFSYILLFAQSYLGSNTAWIGNRRLIIDDEFEKILLSKSYIK
                     EISSARYSEYLTCEYKNLTPIILIAIFENLLDSYLLQNQSEVDLDIFYKVLPLL"
BASE COUNT          186 a           85 c           84 g          169 t
ORIGIN      
        1 aaagcaaagg taaaacttta taaaaaatat ctaagtcaac ctcactctga ttctgaagca
       61 agtaggagtc aagcaaattt tcaaaaatag ctatcaatat gattggtgta aggtttttgt
      121 attcacatgt caagtattct gagtatcgtg cagatgatat ttcttttata taactttttg
      181 aaagcaagat tttttcaaat tcatcatcta ttattaatct tctatttcca atccaagcgg
      241 tattgctacc caaataagat tgtgcaaata gcaatatgta agaaaacatg aacgtaaata
      301 ctgaaagagg tagaattcta tgcgtaaaaa aatagacgat aacggtagcc agaagtaaag
      361 ctatctctcg tgacaatcga attcttttca gcatcttttt taacagtccg taaggttgtc
      421 cttggcaata ttcttgaact aaattaatcg gaatgacatc tctcaccatt tctggaaagt
      481 agagtaagtt tataggttga aatctccagc tcccatcata agta
//
LOCUS       DAVKJD010000174          481 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVKJD010000174 DAVKJD010000000
VERSION     DAVKJD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.174"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..>481
                     /locus_tag="VW157_002020"
     CDS             <1..>481
                     /locus_tag="VW157_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9553257.1"
                     /translation="QGQFEGSKTMEKCYTDVTEFAIPASTQKLYLPPVLDGFNSEIIA
                     FNLSCSPNLEQVQTMLEQAFKEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSR
                     KGNSPDNGMMESFFGILKSEMFYGYEKSFQSFKQLEQAIIDYIDYYNNKRIKVKLKGL
                     "
BASE COUNT          167 a           86 c           94 g          134 t
ORIGIN      
        1 caaggccaat ttgaaggctc taaaacaatg gaaaagtgct acacagatgt gacagaattt
       61 gccattccag caagtactca aaagctttac ttaccaccag ttttagatgg ctttaacagt
      121 gaaattattg cttttaatct ttcttgttcg cctaatttag aacaagtaca aacaatgttg
      181 gaacaggcat tcaaagagaa gcactacgag aatacgattc tccatagtga ccaaggctgg
      241 caatatcaac acgattctta tcatcggttc ctagagagta agggaattca agcatccatg
      301 tcacgtaagg gtaacagccc agacaatggt atgatggagt ccttctttgg gattctgaaa
      361 tcggaaatgt tttacggtta tgagaagtcg tttcagtcgt ttaagcaatt ggaacaagct
      421 attatagact atattgatta ctacaacaat aaacgaatta aggtaaaact aaaaggactt
      481 a
//
LOCUS       DAVKJD010000175          454 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVKJD010000175 DAVKJD010000000
VERSION     DAVKJD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.175"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..>454
                     /locus_tag="VW157_002021"
     CDS             <1..>454
                     /locus_tag="VW157_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HEU9553258.1"
                     /translation="ISYGDMPVIIDFETMLQQPLFDDKTGQSLLDTLFHRVTRTLLLP
                     TEGVKREDGLDVEMSALTGNFKKDAFNGQVLINLNTDKVKFDIGKIDFEGGKNLPVRD
                     GDIEFDKYIKDFKKGFRDFYLIFEELNKTEEFKMLLKANLYGLKTRVL"
BASE COUNT          157 a           47 c          100 g          150 t
ORIGIN      
        1 taatttccta tggagatatg cctgtaataa tagactttga aacaatgtta cagcaacctc
       61 tatttgatga taaaactggt cagagtttgc tggataccct gtttcacagg gtgactcgaa
      121 ctttgcttct tccaacagag ggagtaaaaa gggaggatgg tcttgatgtt gagatgagtg
      181 ctcttacagg aaattttaag aaggatgctt ttaatggtca agttttaatt aatttaaata
      241 ctgataaggt aaagtttgat attgggaaaa ttgattttga aggtgggaaa aacctgcctg
      301 ttcgggatgg tgatatagaa tttgataagt atataaaaga tttcaaaaaa ggttttaggg
      361 atttttatct gatatttgaa gagttaaata aaacagaaga atttaaaatg ctattaaaag
      421 caaacttgta tggtttaaag acaagggtac tttt
//
LOCUS       DAVKJD010000176          439 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVKJD010000176 DAVKJD010000000
VERSION     DAVKJD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.176"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
BASE COUNT          158 a           68 c           66 g          147 t
ORIGIN      
        1 gtttcagatt ctaaactaag tgattcaatc ctttttaagc tatctaaaca cattaaagtc
       61 atcaagcctt aataaactga aagagcctga gtaacaactc aatttcagaa gaaaaaaagt
      121 aaatcttccc acaataaaac gcatagtatc aaggttttta aacacctgat attatgcgtt
      181 tttctgtatc aaagactttt tgcccaattg ctttatctgc cacctcaaag ctatgcattg
      241 agaaatctgc gactcgcttc ctagtttaaa gtggctcttt gattttcatt gagtgaagaa
      301 gatgttgttt taagggtatg taatgatatt tgaaaacctt gatttgatag cctttctacc
      361 taaaataaaa taaatggtaa atagagaaat atgaatttat tgaaatttaa tcagttagga
      421 atcagttacg ataaattaa
//
LOCUS       DAVKJD010000177          413 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVKJD010000177 DAVKJD010000000
VERSION     DAVKJD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.177"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..220
                     /locus_tag="VW157_002022"
     CDS             <1..220
                     /locus_tag="VW157_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9553259.1"
                     /translation="EVGEISHRISEGGLWLDDYYLFHETLRDYRNKQSKEKQEELERV
                     LSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            314..385
                     /locus_tag="VW157_002023"
     tRNA            314..385
                     /locus_tag="VW157_002023"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:347..349,aa:Glu,seq:ttc)
BASE COUNT          149 a           63 c           99 g          102 t
ORIGIN      
        1 agaagtgggt gagatcagtc atcgtataag tgaggggggt ctctggctag atgattatta
       61 tctctttcat gaaacactaa gagattatag aaacaaacaa agtaaagaga aacaagaaga
      121 actagaacgc gtcttaagca atgaacgatt tcgagggcgt caaagagtat taagagactt
      181 acgcattgtg tttaaggagt ttactatccg tacccattaa taagtcatgc aaaaaaaatg
      241 aaaaaaatta gaaaaagtag ttgacaaagt ttgaaaaggc tgtataatag taagagttga
      301 aaataacaac tcaggtccgt tggtcaaggg gttaagacac cgccttttca cggcggtaac
      361 acgggttcga atcccgtacg gactatggta tgttgcgtca ggaccacttg atg
//
LOCUS       DAVKJD010000178          400 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVKJD010000178 DAVKJD010000000
VERSION     DAVKJD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.178"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            186..>400
                     /locus_tag="VW157_002024"
     CDS             186..>400
                     /locus_tag="VW157_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEU9553260.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAA"
BASE COUNT          149 a           48 c           97 g          106 t
ORIGIN      
        1 tagtaaaaaa gtgattagaa aacatctttt ttaaaaatag agatgatttt gaaacaaaaa
       61 agctaattca agacgtttcg atgccaattc aagatttgga tgaaaaaaat taatagatac
      121 tgttatacta aacttgtcaa gtttgtaaca agacaaatat taaaaataaa aaagaggtat
      181 tcgttatgaa tacaaaaaag atgtcacaat ttgaaattat ggatactgag atgcttgctt
      241 gcgttgaagg tggcggatgc aattggggag attttgccaa agcaggtgtt ggaggaggag
      301 cagtacgagg tcttcagcta ggaattaaaa caagaacatg gcaaggtgca gcaactggtg
      361 ctgcgggagg agctatactt ggaggtgtgg cctatgcagc
//
LOCUS       DAVKJD010000179          397 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVKJD010000179 DAVKJD010000000
VERSION     DAVKJD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.179"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            1..397
                     /locus_tag="VW157_002025"
                     /pseudo
     CDS             1..397
                     /locus_tag="VW157_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
BASE COUNT          125 a           74 c           78 g          120 t
ORIGIN      
        1 taactctttg atcaggtagc aagcttgttg gaagaacgag aaattgtcac tcttacttat
       61 cgtgaagcct tgatcacgcg tgaaaagtca tttccaactg gtttagatat ggaatttcta
      121 ggaaaggact tgccaaatgt agcgattcct cacacagata ttgttcacaa tctggctgaa
      181 aaagttgtgg tcgttcgatt agagaaaccg gtgactttcc ataatatgat tgcgccaggt
      241 aaggaagtag aagtatcctt gctcttcttt atcatcaata actcaagttc aagtcaaaca
      301 aatattctgg ctcagttgat ggactttttc acaggaaatg gacatcttga agacctatca
      361 aaaatttccg aaccagaaaa actttatgct tacattg
//
LOCUS       DAVKJD010000180          371 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVKJD010000180 DAVKJD010000000
VERSION     DAVKJD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.180"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            complement(1..326)
                     /locus_tag="VW157_002026"
                     /pseudo
     CDS             complement(1..326)
                     /locus_tag="VW157_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          109 a           70 c           52 g          140 t
ORIGIN      
        1 cgttgacaat ctatgagcta taattatttg agtacaaccc tgactttgta tatactttgt
       61 tattctttcc tcattaatag tgtctaatgc actagttgct tcatctaaaa ttacaatact
      121 aggattattt attaatgcac gtgccagtgc tatccgttgc ctttgcccac ctgaaatatt
      181 tgaccccatc tctgagatga tagtattaaa tttcatcggc atagccatga tttcatcata
      241 gatttgaact gctttacaaa cttcctctat cttttgtgaa gtaactagtg ctttaaagtt
      301 atgttatcaa gaatacttcg gttcaataaa aatgaatcct gtggaactac tcctaaattt
      361 tgacttaata t
//
LOCUS       DAVKJD010000181          353 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVKJD010000181 DAVKJD010000000
VERSION     DAVKJD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.181"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..>353
                     /locus_tag="VW157_002027"
     CDS             <1..>353
                     /locus_tag="VW157_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9553261.1"
                     /translation="SNPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGI
                     DSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDLLDNKGRQVDFEALDN
                     LLERLKDVSSDKVKL"
BASE COUNT          120 a           60 c           80 g           93 t
ORIGIN      
        1 caagcaatcc aattgatgag aaattggtca aagaagctgt tcgaaaagta ggcgatggtt
       61 atgtctttga ggagaatgga gtttctcgtt atatcccagc caaggatctt tcagcagaaa
      121 cagcagcagg cattgatagc aaactggcca agcaggaaag tttatctcat aagctaggag
      181 ctaagaaaac tgacctccca tctagtgatc gagaatttta caataaggct tatgacttac
      241 tagcaagaat tcaccaagat ttacttgata ataaaggtcg acaagttgat tttgaggctt
      301 tggataacct gttggaacga ctcaaggatg tctcaagtga taaagtcaag tta
//
LOCUS       DAVKJD010000182          353 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVKJD010000182 DAVKJD010000000
VERSION     DAVKJD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.182"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            145..>353
                     /locus_tag="VW157_002028"
     CDS             145..>353
                     /locus_tag="VW157_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9553262.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQ"
BASE COUNT          110 a           72 c           57 g          114 t
ORIGIN      
        1 ctaaaaacca tccagaatcc ttgccttagc ttagatcctg gatggtttct tttttcaccc
       61 aatgggtgtt ttttactaga caaaaaagag tttccccttt atggtataag tgtagaaaaa
      121 aacacaaaaa gaaaggaaac tcacatgaac agtttaccaa atcatcactt ccaaaacaag
      181 tctttttacc aactatcttt cgatggaggt catttaaccc agtatggtgg tcttatcttt
      241 tttcaggaac ttttttccca gttgaaacta aaagagcgga tttctaagta tttagtaacg
      301 aatgaccaac gccgctactg tcgttattcg gattcagata tccttgtcca gtt
//
LOCUS       DAVKJD010000183          341 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVKJD010000183 DAVKJD010000000
VERSION     DAVKJD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.183"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..>341
                     /locus_tag="VW157_002029"
     CDS             <1..>341
                     /locus_tag="VW157_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9553263.1"
                     /translation="RLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGD
                     AYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYN
                     RVKAAKKVPLD"
BASE COUNT          116 a           71 c           82 g           72 t
ORIGIN      
        1 aacgtttagg aaaaccaaat gcgcaaatta cctacactga tgatgagatt caagtagcca
       61 agttggcagg caagtacaca acagaagacg gttatatctt tgatcctcgt gatataacca
      121 gtgatgaggg ggatgcctat gtaactccac atatgaccca tagccactgg attaaaaaag
      181 atagtttgtc tgaagctgag agagcggcag cccaggctta tgctaaagag aaaggtttga
      241 cccctccttc gacagaccat caggattcag gaaatactga ggcaaaagga gcagaagcta
      301 tctacaaccg cgtgaaagca gctaagaagg tgccacttga t
//
LOCUS       DAVKJD010000184          295 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVKJD010000184 DAVKJD010000000
VERSION     DAVKJD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.184"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
BASE COUNT          106 a           36 c           52 g          101 t
ORIGIN      
        1 tttataagga agatggaacc agcgtcacag ttcgtgctta atgtagttgt ttagttttaa
       61 aaaattatgc aaagctccat atacaacact taaacgagat taaagaatgg cgaaatccct
      121 tgatgcgcaa gagattagct gtcctttttt attttttaag tgataaagtc ggagcttagg
      181 tatcaaagcc tatcaaatta aacaaagaag tgatgtctta gatattttga aaaaaattaa
      241 taagcagaaa actctctatt attttgttgt agagagtttt ttgttaataa aattt
//
LOCUS       DAVKJD010000185          293 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00073014
            SAMN33606118-rid17941313.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVKJD010000185 DAVKJD010000000
VERSION     DAVKJD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606118
            Sequence Read Archive: SRR23727437
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722479.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722479.1
            Genome Coverage       :: 300x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 23:55:34
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,030
            CDSs (total)                      :: 1,983
            Genes (coding)                    :: 1,868
            CDSs (with protein)               :: 1,868
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 115
            CDSs (without protein)            :: 115
            Pseudo Genes (ambiguous residues) :: 0 of 115
            Pseudo Genes (frameshifted)       :: 54 of 115
            Pseudo Genes (incomplete)         :: 58 of 115
            Pseudo Genes (internal stop)      :: 36 of 115
            Pseudo Genes (multiple problems)  :: 28 of 115
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606118-rid17941313.denovo.185"
                     /strain="AUSMDU00073014"
                     /serotype="12F"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST218"
     gene            <1..>293
                     /locus_tag="VW157_002030"
     CDS             <1..>293
                     /locus_tag="VW157_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEU9553264.1"
                     /translation="QALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTINI
                     NGLISKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVNHVIPYELFAGDG"
BASE COUNT          104 a           64 c           59 g           66 t
ORIGIN      
        1 caagcgctca ttgaccagtt ccgagcaaat ggtactcaaa cttacagcgc tacagtcaat
       61 gtctatggta acaaagacgg taaaccagac ttggacaaca tcgtagcaac taaaaaagtc
      121 actattaaca taaacggttt aatttctaaa gaaacagttc aaaaagccgt tgcagataac
      181 gttaaagata gtatcgatgt tccagcagcc tacctagaaa aagccaaggg tgaaggtcca
      241 ttcacagcag gtgtcaacca tgtgattcca tacgaactct tcgcaggtga tgg
//
